JPS557005A - Protein adsorbent and purification of urokinase with it - Google Patents
Protein adsorbent and purification of urokinase with itInfo
- Publication number
- JPS557005A JPS557005A JP7743878A JP7743878A JPS557005A JP S557005 A JPS557005 A JP S557005A JP 7743878 A JP7743878 A JP 7743878A JP 7743878 A JP7743878 A JP 7743878A JP S557005 A JPS557005 A JP S557005A
- Authority
- JP
- Japan
- Prior art keywords
- urokinase
- adsorbent
- bonded
- purified
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 title abstract 4
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title abstract 4
- 239000003463 adsorbent Substances 0.000 title abstract 4
- 229960005356 urokinase Drugs 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 125000002490 anilino group Chemical group [H]N(*)C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 125000003282 alkyl amino group Chemical group 0.000 abstract 1
- KUDPGZONDFORKU-UHFFFAOYSA-N n-chloroaniline Chemical group ClNC1=CC=CC=C1 KUDPGZONDFORKU-UHFFFAOYSA-N 0.000 abstract 1
- 125000001453 quaternary ammonium group Chemical group 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
PURPOSE: To obtain purified urokinase in high yield and purity, by adsorbing a crude urokinase solution with an adsorbent comprising a carrier bonded to an aniline derivative.
CONSTITUTION: Crude urokinase (U) is adsorbed to a packed bed of a protein adsorbent, wherein an aniline derivative of formula I: (X is alkylamino or alkyl- substituted quaternary ammonium) is bonded to a water-insoluble carrier directly or via a mediator, and then eluted with a saline solution to give purified U. The solution containing the purified U is passed through an adsorbent bonded to chloroaniline for further advantageous purification.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7743878A JPS5834179B2 (en) | 1978-06-28 | 1978-06-28 | Protein adsorbent and method for purifying urokinase using the protein adsorbent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7743878A JPS5834179B2 (en) | 1978-06-28 | 1978-06-28 | Protein adsorbent and method for purifying urokinase using the protein adsorbent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS557005A true JPS557005A (en) | 1980-01-18 |
| JPS5834179B2 JPS5834179B2 (en) | 1983-07-25 |
Family
ID=13634016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7743878A Expired JPS5834179B2 (en) | 1978-06-28 | 1978-06-28 | Protein adsorbent and method for purifying urokinase using the protein adsorbent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5834179B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57197925U (en) * | 1981-06-12 | 1982-12-15 |
-
1978
- 1978-06-28 JP JP7743878A patent/JPS5834179B2/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57197925U (en) * | 1981-06-12 | 1982-12-15 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5834179B2 (en) | 1983-07-25 |
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