JPS5570741A - Substrate analysis method - Google Patents
Substrate analysis methodInfo
- Publication number
- JPS5570741A JPS5570741A JP14456778A JP14456778A JPS5570741A JP S5570741 A JPS5570741 A JP S5570741A JP 14456778 A JP14456778 A JP 14456778A JP 14456778 A JP14456778 A JP 14456778A JP S5570741 A JPS5570741 A JP S5570741A
- Authority
- JP
- Japan
- Prior art keywords
- dialysis
- buffer solution
- chamber
- specimen
- flow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004458 analytical method Methods 0.000 title abstract 3
- 239000000758 substrate Substances 0.000 title abstract 3
- 238000000502 dialysis Methods 0.000 abstract 10
- 239000007853 buffer solution Substances 0.000 abstract 7
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To extend the range possible for analysis, by leading the buffer solution in two flows into a pair of dialysis chamber of the dialysis unit sectioned with dialysis film, and making greater the flow of the buffer solution of the side passing through the fixed enzyme column.
CONSTITUTION: The buffer solution 2 is fed to the dialysis chamber 23 of the dialysis unit 20 and the inlet of specimen 4 with the delivery pumps 3a and 3b. The buffer solution 2 through the inlet of specimen 4 is discharged through the dialysis chamber 22. The buffer solution 2 including the substrate and through the dialysis chamber B23 passes through the fixed enzyme column 7 and after it is detected at the engyme detector 10, it is discharged as the discharge liquid 11b. The reduction in the engyme detected analyzes the substrate in the specimen. The dialysis chamber 20 is sectioned with the dialysis film 21 and the flow of the buffer solution of the dialysis chamber B23 is taken greater than that of the chamber A22. Thus, the flow of the buffer solution in the column is increased to extend the range of possible analysis.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14456778A JPS5570741A (en) | 1978-11-21 | 1978-11-21 | Substrate analysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14456778A JPS5570741A (en) | 1978-11-21 | 1978-11-21 | Substrate analysis method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5570741A true JPS5570741A (en) | 1980-05-28 |
| JPS6120279B2 JPS6120279B2 (en) | 1986-05-21 |
Family
ID=15365221
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14456778A Granted JPS5570741A (en) | 1978-11-21 | 1978-11-21 | Substrate analysis method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5570741A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59130196A (en) * | 1983-01-11 | 1984-07-26 | Sekisui Chem Co Ltd | Determination of biocomponent using immobilized enzyme |
| JPH01156664A (en) * | 1987-12-15 | 1989-06-20 | New Japan Radio Co Ltd | Instrument for measuring freshness of meat |
| JPH01178861A (en) * | 1988-01-08 | 1989-07-17 | Taiyo Fishery Co Ltd | Sensor for measuring freshness |
-
1978
- 1978-11-21 JP JP14456778A patent/JPS5570741A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59130196A (en) * | 1983-01-11 | 1984-07-26 | Sekisui Chem Co Ltd | Determination of biocomponent using immobilized enzyme |
| JPH01156664A (en) * | 1987-12-15 | 1989-06-20 | New Japan Radio Co Ltd | Instrument for measuring freshness of meat |
| JPH01178861A (en) * | 1988-01-08 | 1989-07-17 | Taiyo Fishery Co Ltd | Sensor for measuring freshness |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6120279B2 (en) | 1986-05-21 |
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