JPS5574796A - Separation of acidic polysaccharides - Google Patents
Separation of acidic polysaccharidesInfo
- Publication number
- JPS5574796A JPS5574796A JP14650878A JP14650878A JPS5574796A JP S5574796 A JPS5574796 A JP S5574796A JP 14650878 A JP14650878 A JP 14650878A JP 14650878 A JP14650878 A JP 14650878A JP S5574796 A JPS5574796 A JP S5574796A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- hyaluronic acid
- chondroitin sulfate
- treated
- animal tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001284 acidic polysaccharide Polymers 0.000 title 1
- 150000004805 acidic polysaccharides Chemical class 0.000 title 1
- 238000000926 separation method Methods 0.000 title 1
- 239000000243 solution Substances 0.000 abstract 6
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 abstract 4
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 abstract 4
- 229920001287 Chondroitin sulfate Polymers 0.000 abstract 4
- 229940059329 chondroitin sulfate Drugs 0.000 abstract 4
- 229920002674 hyaluronan Polymers 0.000 abstract 4
- 229960003160 hyaluronic acid Drugs 0.000 abstract 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 108091005804 Peptidases Proteins 0.000 abstract 2
- 239000004365 Protease Substances 0.000 abstract 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 239000000706 filtrate Substances 0.000 abstract 2
- 241000561734 Celosia cristata Species 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 210000001520 comb Anatomy 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 125000001453 quaternary ammonium group Chemical group 0.000 abstract 1
- 150000003242 quaternary ammonium salts Chemical class 0.000 abstract 1
- 239000012266 salt solution Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
PURPOSE: To separate hyaluronic acid and chondroitin sulfate from animal tissue, easily, in high yield, by heating the animal tissue in a solution of a quaternary ammonium salt, and treating with protease to separate hyaluronic acid, and then treating the residue with a salt solution to obtain chondroitin sulfate.
CONSTITUTION: Animal tissue such as cockscombs, naval cords, skin, etc. is heated in an aqueous solution of a quaternary ammonium slat, cut to pieces, added with water, and treated with protease. The treated solution is filtered to concentrate hyaluronic acid in the filtrate (solution A). The residue is treated with a solution of a salt such as NaCl or of an alkali such as NaOH, to collect chondroitin sulfate in the filtrate (solution B). The hyaluronic acid and the chondroitin sulfate are precipitated by adding an alcohol to the solutions A and B.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14650878A JPS5574796A (en) | 1978-11-29 | 1978-11-29 | Separation of acidic polysaccharides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14650878A JPS5574796A (en) | 1978-11-29 | 1978-11-29 | Separation of acidic polysaccharides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5574796A true JPS5574796A (en) | 1980-06-05 |
| JPS6121241B2 JPS6121241B2 (en) | 1986-05-26 |
Family
ID=15409207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14650878A Granted JPS5574796A (en) | 1978-11-29 | 1978-11-29 | Separation of acidic polysaccharides |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5574796A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000035959A1 (en) * | 1998-12-17 | 2000-06-22 | Fujii Suisan Co., Ltd. | Method for processing fish heads and apparatus for separating processed fish heads |
| JP2010124811A (en) * | 2008-12-01 | 2010-06-10 | Daimatsu:Kk | Volume reduction extraction method of glycosaminoglycan and generation method of proteoglycan-containing precipitation |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6371829U (en) * | 1986-10-30 | 1988-05-13 |
-
1978
- 1978-11-29 JP JP14650878A patent/JPS5574796A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000035959A1 (en) * | 1998-12-17 | 2000-06-22 | Fujii Suisan Co., Ltd. | Method for processing fish heads and apparatus for separating processed fish heads |
| US6347986B1 (en) | 1998-12-17 | 2002-02-19 | Fujii Suisan Co., Ltd. | Method for processing fish heads and apparatus for separating processed fish heads |
| JP2010124811A (en) * | 2008-12-01 | 2010-06-10 | Daimatsu:Kk | Volume reduction extraction method of glycosaminoglycan and generation method of proteoglycan-containing precipitation |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6121241B2 (en) | 1986-05-26 |
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