JPS5592355A - Enzyme-adsorbing substance and purification of enzyme using the same - Google Patents
Enzyme-adsorbing substance and purification of enzyme using the sameInfo
- Publication number
- JPS5592355A JPS5592355A JP15754278A JP15754278A JPS5592355A JP S5592355 A JPS5592355 A JP S5592355A JP 15754278 A JP15754278 A JP 15754278A JP 15754278 A JP15754278 A JP 15754278A JP S5592355 A JPS5592355 A JP S5592355A
- Authority
- JP
- Japan
- Prior art keywords
- compound
- enzyme
- formula
- adsorbing substance
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 239000000126 substance Substances 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 6
- 229940088598 enzyme Drugs 0.000 abstract 2
- YNMSLFTYGNUVKT-UHFFFAOYSA-N 2-(4-aminophenoxy)ethanimidamide hydrochloride Chemical compound Cl.NC(=N)COC1=CC=C(N)C=C1 YNMSLFTYGNUVKT-UHFFFAOYSA-N 0.000 abstract 1
- 229920000936 Agarose Polymers 0.000 abstract 1
- 102000001399 Kallikrein Human genes 0.000 abstract 1
- 108060005987 Kallikrein Proteins 0.000 abstract 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 abstract 1
- 102000004142 Trypsin Human genes 0.000 abstract 1
- 108090000631 Trypsin Proteins 0.000 abstract 1
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 abstract 1
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 abstract 1
- 125000000217 alkyl group Chemical group 0.000 abstract 1
- 125000002947 alkylene group Chemical group 0.000 abstract 1
- 150000001409 amidines Chemical class 0.000 abstract 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N anhydrous guanidine Natural products NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 abstract 1
- 239000011230 binding agent Substances 0.000 abstract 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 abstract 1
- -1 guanidine compound Chemical class 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 125000000896 monocarboxylic acid group Chemical group 0.000 abstract 1
- 125000000843 phenylene group Chemical group C1(=C(C=CC=C1)*)* 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 239000012588 trypsin Substances 0.000 abstract 1
- 229960005356 urokinase Drugs 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
NEW MATERIAL:Amidine or guanidine compound of formula I [X is NH2 or COOH; A is 1W4C alkylene or phenylene; R is a group of formula II or formula III (γ1Wγ3 are H or lower alkyl], and a water-insoluble carrier bonded with the above compound directly or through a binder.
EXAMPLE: 2-(4-Aminophenoxy)acetamidine hydrochloride.
USE: Enzyme-adsorbing substance and its raw material, having a specific affinity with enzymes, e.g. urokinase, trypsin, kallikrein, etc. Useful for the purification of the above enzymes.
PROCESS: For example, a compound of formula I such as a compound of formula V can be obtained from a compound of formula IV. The compound obtained is then bonded with a water-insoluble carrier (preferably agarose) directly or through a bonding compound to given an enzyme-adsorbing substance.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15754278A JPS5592355A (en) | 1978-12-22 | 1978-12-22 | Enzyme-adsorbing substance and purification of enzyme using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15754278A JPS5592355A (en) | 1978-12-22 | 1978-12-22 | Enzyme-adsorbing substance and purification of enzyme using the same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5592355A true JPS5592355A (en) | 1980-07-12 |
Family
ID=15651947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15754278A Pending JPS5592355A (en) | 1978-12-22 | 1978-12-22 | Enzyme-adsorbing substance and purification of enzyme using the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5592355A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59109172A (en) * | 1982-12-13 | 1984-06-23 | Ss Pharmaceut Co Ltd | Purification of kallikrein |
| JPS6485077A (en) * | 1987-09-25 | 1989-03-30 | Takara Shuzo Co | Purification of arginine residue-specific endopeptidase |
-
1978
- 1978-12-22 JP JP15754278A patent/JPS5592355A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59109172A (en) * | 1982-12-13 | 1984-06-23 | Ss Pharmaceut Co Ltd | Purification of kallikrein |
| JPS6485077A (en) * | 1987-09-25 | 1989-03-30 | Takara Shuzo Co | Purification of arginine residue-specific endopeptidase |
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