JPS6485077A - Purification of arginine residue-specific endopeptidase - Google Patents
Purification of arginine residue-specific endopeptidaseInfo
- Publication number
- JPS6485077A JPS6485077A JP24021287A JP24021287A JPS6485077A JP S6485077 A JPS6485077 A JP S6485077A JP 24021287 A JP24021287 A JP 24021287A JP 24021287 A JP24021287 A JP 24021287A JP S6485077 A JPS6485077 A JP S6485077A
- Authority
- JP
- Japan
- Prior art keywords
- arginine residue
- specific endopeptidase
- buffer solution
- purification
- endopeptidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000005593 Endopeptidases Human genes 0.000 title abstract 6
- 108010059378 Endopeptidases Proteins 0.000 title abstract 6
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 title abstract 6
- 238000000746 purification Methods 0.000 title 1
- 239000007853 buffer solution Substances 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE:To purify an arginine residue-specific endopeptidase in high yield, by adsorbing an arginine residue-specific endopeptidase to a benzamidine- immobilized carrier to remove foreign matters and eluting the adsorbed enzyme. CONSTITUTION:An arginine residue-specific endopeptidase is made to contact with a benzamidine-immobilized carrier in a 0.01-0.1M phosphoric acid buffer solution of pH7-8 to effect the adsorption of the arginine residue-specific endopeptidase and remove foreign matters. The adsorbed enzyme is eluted with a buffer solution produced by adding about 1M of sodium chloride to the above buffer solution and then eluted with an acid solution of pH2-3, e.g. about 1mM of hydrochloric acid. The purified arginine residue-specific endopeptidase exhibits single band in polyacrylamide electrophoresis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24021287A JPS6485077A (en) | 1987-09-25 | 1987-09-25 | Purification of arginine residue-specific endopeptidase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24021287A JPS6485077A (en) | 1987-09-25 | 1987-09-25 | Purification of arginine residue-specific endopeptidase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6485077A true JPS6485077A (en) | 1989-03-30 |
Family
ID=17056120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24021287A Pending JPS6485077A (en) | 1987-09-25 | 1987-09-25 | Purification of arginine residue-specific endopeptidase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6485077A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5395190A (en) * | 1977-01-31 | 1978-08-19 | Nippon Soda Co Ltd | Production of urokinase adsorbent and high purity urokinase |
| JPS5517364A (en) * | 1978-07-25 | 1980-02-06 | Toho Yakuhin Kogyo Kk | Purification of pancreatic kallikrein |
| JPS5592355A (en) * | 1978-12-22 | 1980-07-12 | Nippon Soda Co Ltd | Enzyme-adsorbing substance and purification of enzyme using the same |
-
1987
- 1987-09-25 JP JP24021287A patent/JPS6485077A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5395190A (en) * | 1977-01-31 | 1978-08-19 | Nippon Soda Co Ltd | Production of urokinase adsorbent and high purity urokinase |
| JPS5517364A (en) * | 1978-07-25 | 1980-02-06 | Toho Yakuhin Kogyo Kk | Purification of pancreatic kallikrein |
| JPS5592355A (en) * | 1978-12-22 | 1980-07-12 | Nippon Soda Co Ltd | Enzyme-adsorbing substance and purification of enzyme using the same |
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