JPS5592696A - Reagent for detection of peroxidase - Google Patents
Reagent for detection of peroxidaseInfo
- Publication number
- JPS5592696A JPS5592696A JP16125378A JP16125378A JPS5592696A JP S5592696 A JPS5592696 A JP S5592696A JP 16125378 A JP16125378 A JP 16125378A JP 16125378 A JP16125378 A JP 16125378A JP S5592696 A JPS5592696 A JP S5592696A
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- naphthol
- buffer solution
- peroxidase
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 3
- 102000003992 Peroxidases Human genes 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title abstract 2
- 108040007629 peroxidase activity proteins Proteins 0.000 title abstract 2
- -1 naphthol compound Chemical class 0.000 abstract 3
- 239000007853 buffer solution Substances 0.000 abstract 2
- HVLJEMXDXOTWLV-UHFFFAOYSA-N 2,4-dichloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=C(Cl)C=C(Cl)C2=C1 HVLJEMXDXOTWLV-UHFFFAOYSA-N 0.000 abstract 1
- 229910006080 SO2X Inorganic materials 0.000 abstract 1
- 229910052783 alkali metal Inorganic materials 0.000 abstract 1
- 150000001340 alkali metals Chemical class 0.000 abstract 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 abstract 1
- 229910052736 halogen Inorganic materials 0.000 abstract 1
- 150000002367 halogens Chemical class 0.000 abstract 1
- 150000007857 hydrazones Chemical class 0.000 abstract 1
- OEZPVSPULCMUQB-VRTOBVRTSA-N hydron;(e)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine;chloride Chemical compound Cl.C1=CC=C2S\C(=N\N)N(C)C2=C1 OEZPVSPULCMUQB-VRTOBVRTSA-N 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To provide a reagent for the detection of peroxidase, having high performance comparable to benzidine, and prepared by dissolving a specific hydrazone and a naphthol compound in a buffer solution.
CONSTITUTION: The title reagent is obtained by dissolving (A) 0.5W5m-mol of 3-methyl-2-benzothiazolinone hydrazone hydrochloride and (B) 3W25m-mol of a naphthol compound of formula (A is COOX, SO2X, halogen; X is H or alkali metal; m is 0, 1, or 2; n is 0 or 1), e.g. 2-amino-5-naphthol-7-sulfonic acid sodium salt, 2,4-dichloro-1-naphthol, etc., in (C) a buffer solution, e.g. a phosphate buffer solution, and adjusting the pH of the mixture to 5W7.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16125378A JPS5592696A (en) | 1978-12-28 | 1978-12-28 | Reagent for detection of peroxidase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16125378A JPS5592696A (en) | 1978-12-28 | 1978-12-28 | Reagent for detection of peroxidase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5592696A true JPS5592696A (en) | 1980-07-14 |
| JPS6176B2 JPS6176B2 (en) | 1986-01-06 |
Family
ID=15731565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16125378A Granted JPS5592696A (en) | 1978-12-28 | 1978-12-28 | Reagent for detection of peroxidase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5592696A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5664798A (en) * | 1979-10-29 | 1981-06-02 | Nippon Koutai Kenkyusho:Kk | Diagnostic method of cancer |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61221681A (en) * | 1985-03-27 | 1986-10-02 | Hitachi Electronics Eng Co Ltd | Ic inspection system |
-
1978
- 1978-12-28 JP JP16125378A patent/JPS5592696A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5664798A (en) * | 1979-10-29 | 1981-06-02 | Nippon Koutai Kenkyusho:Kk | Diagnostic method of cancer |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6176B2 (en) | 1986-01-06 |
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