JPS56102787A - Preparation of human placenta superoxide dismutase - Google Patents
Preparation of human placenta superoxide dismutaseInfo
- Publication number
- JPS56102787A JPS56102787A JP348280A JP348280A JPS56102787A JP S56102787 A JPS56102787 A JP S56102787A JP 348280 A JP348280 A JP 348280A JP 348280 A JP348280 A JP 348280A JP S56102787 A JPS56102787 A JP S56102787A
- Authority
- JP
- Japan
- Prior art keywords
- superoxide
- dismutase
- human placenta
- precipitate
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PURPOSE: To separate superoxide.dismutase from the supernatant liquid obtained by disintegrating the tissue of human placenta with an aqeous solution of potassium chloride or sodium chloride.
CONSTITUTION: The sufficiently blooded fresh human placenta obtained through normal delivery, or its frozen product is added with 5 times weigth of 1.15% potassium chloride solution or 0.9% sodium chloride solution, and maintained at 0W4°C to effect complete disintegration of the tissue. The precipitate is removed therfrom by centrifugal separation, and the supernatant liquid is heated at about 60W75°C for about 5min. The produced precipitate is removed by filtration, and the filtrate is mixed with ammonium solfate solution of 50W90% saturation. The precipitate is dissolved and dialyzed, and the dialyzate is subjected to ion exchange Sephadex chromatography or treated with a carrier bonded with superoxide.dismutase antibody, to obtain mitochondrian.superoxide.dismutase and soluble fraction of superoxide.dismutase.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP348280A JPS56102787A (en) | 1980-01-18 | 1980-01-18 | Preparation of human placenta superoxide dismutase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP348280A JPS56102787A (en) | 1980-01-18 | 1980-01-18 | Preparation of human placenta superoxide dismutase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS56102787A true JPS56102787A (en) | 1981-08-17 |
Family
ID=11558548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP348280A Pending JPS56102787A (en) | 1980-01-18 | 1980-01-18 | Preparation of human placenta superoxide dismutase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56102787A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5925682A (en) * | 1982-08-04 | 1984-02-09 | Toyo Soda Mfg Co Ltd | Separation and purification of cu, zn-superoxide dismutase |
| JPS62289187A (en) * | 1985-11-22 | 1987-12-16 | バイオ−テクノロジ−・ジエネラル・コ−ポレイシヨン | Human manganese superoxide dismutase cdna, it development inbacteria and recovery of enzyme active human manganese superoxide dismutase |
| US5130245A (en) * | 1985-09-03 | 1992-07-14 | Stefan Marklund | Superoxide dismutase |
| US5248603A (en) * | 1985-09-03 | 1993-09-28 | Symbicom Aktiebolag | Superoxide dismutase |
-
1980
- 1980-01-18 JP JP348280A patent/JPS56102787A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5925682A (en) * | 1982-08-04 | 1984-02-09 | Toyo Soda Mfg Co Ltd | Separation and purification of cu, zn-superoxide dismutase |
| US5130245A (en) * | 1985-09-03 | 1992-07-14 | Stefan Marklund | Superoxide dismutase |
| US5248603A (en) * | 1985-09-03 | 1993-09-28 | Symbicom Aktiebolag | Superoxide dismutase |
| US5472691A (en) * | 1985-09-03 | 1995-12-05 | Symbicom Aktiebolag | Superoxide dismutase |
| US5788961A (en) * | 1985-09-03 | 1998-08-04 | Symbicom Aktiebolag | Superoxide dismutase |
| JPS62289187A (en) * | 1985-11-22 | 1987-12-16 | バイオ−テクノロジ−・ジエネラル・コ−ポレイシヨン | Human manganese superoxide dismutase cdna, it development inbacteria and recovery of enzyme active human manganese superoxide dismutase |
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