JPS5653460A - Analyzing method for constituent of low molecular weight - Google Patents

Analyzing method for constituent of low molecular weight

Info

Publication number
JPS5653460A
JPS5653460A JP12944779A JP12944779A JPS5653460A JP S5653460 A JPS5653460 A JP S5653460A JP 12944779 A JP12944779 A JP 12944779A JP 12944779 A JP12944779 A JP 12944779A JP S5653460 A JPS5653460 A JP S5653460A
Authority
JP
Japan
Prior art keywords
constituent
molecular weight
low molecular
sample
column
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12944779A
Other languages
Japanese (ja)
Other versions
JPH02666B2 (en
Inventor
Isao Kaiho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Resonac Holdings Corp
Original Assignee
Showa Denko KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Showa Denko KK filed Critical Showa Denko KK
Priority to JP12944779A priority Critical patent/JPS5653460A/en
Publication of JPS5653460A publication Critical patent/JPS5653460A/en
Publication of JPH02666B2 publication Critical patent/JPH02666B2/ja
Granted legal-status Critical Current

Links

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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE: To conduct the liquid chromatography of the constituent of low molecular weight without deteriorating the performance of a separating column by a method wherein the constituent of high molecular weight is removed beforehand by using a water-size separating column and the constituent of low molecular weight alone is introduced into the main column for separation.
CONSTITUTION: First, a sample for measuring the flow-out velocity is injected in the eluent and is introduced into the water-size separating column, and then the time from injection of the sample to the point of time when the flow-out of the constituent of high molecular weight in the sample from the column is completed and the constituent of low molecular weight starts to flow out is measured beforehand. Next, the sample to be measured is injected into the eluent and then passed through the water-size separating column together with the eluent, and there the constituent which flows out by the time measured beforehand is removed. And, the constituent which flows out after the lapse of a prescribed time alone is introduced into the main column for sepatation and thus the analysis of the constituent of low molecular weight is performed with a liquid chromatography.
COPYRIGHT: (C)1981,JPO&Japio
JP12944779A 1979-10-09 1979-10-09 Analyzing method for constituent of low molecular weight Granted JPS5653460A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP12944779A JPS5653460A (en) 1979-10-09 1979-10-09 Analyzing method for constituent of low molecular weight

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12944779A JPS5653460A (en) 1979-10-09 1979-10-09 Analyzing method for constituent of low molecular weight

Publications (2)

Publication Number Publication Date
JPS5653460A true JPS5653460A (en) 1981-05-13
JPH02666B2 JPH02666B2 (en) 1990-01-09

Family

ID=15009695

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12944779A Granted JPS5653460A (en) 1979-10-09 1979-10-09 Analyzing method for constituent of low molecular weight

Country Status (1)

Country Link
JP (1) JPS5653460A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60159375U (en) * 1984-03-30 1985-10-23 株式会社島津製作所 liquid chromatograph
JPH0269657A (en) * 1988-07-27 1990-03-08 Bio Rad Lab Inc Wide-area spectral medicine detection system

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5338958A (en) * 1976-09-22 1978-04-10 Nippon Gakki Seizo Kk Monostable multi-vibrator circuit
JPS53149092A (en) * 1977-05-31 1978-12-26 Hitachi Ltd Analysis of amino acid

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5338958A (en) * 1976-09-22 1978-04-10 Nippon Gakki Seizo Kk Monostable multi-vibrator circuit
JPS53149092A (en) * 1977-05-31 1978-12-26 Hitachi Ltd Analysis of amino acid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60159375U (en) * 1984-03-30 1985-10-23 株式会社島津製作所 liquid chromatograph
JPH0269657A (en) * 1988-07-27 1990-03-08 Bio Rad Lab Inc Wide-area spectral medicine detection system

Also Published As

Publication number Publication date
JPH02666B2 (en) 1990-01-09

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