JPS57166559A - Latex reagent and measuring method for latex coagulation reaction - Google Patents

Latex reagent and measuring method for latex coagulation reaction

Info

Publication number
JPS57166559A
JPS57166559A JP5277981A JP5277981A JPS57166559A JP S57166559 A JPS57166559 A JP S57166559A JP 5277981 A JP5277981 A JP 5277981A JP 5277981 A JP5277981 A JP 5277981A JP S57166559 A JPS57166559 A JP S57166559A
Authority
JP
Japan
Prior art keywords
latex
styrene
antiserum
reagent
sensitized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP5277981A
Other languages
Japanese (ja)
Other versions
JPS6352708B2 (en
Inventor
Toichi Yamada
Michio Kuge
Satoshi Obana
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Priority to JP5277981A priority Critical patent/JPS57166559A/en
Publication of JPS57166559A publication Critical patent/JPS57166559A/en
Publication of JPS6352708B2 publication Critical patent/JPS6352708B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

PURPOSE:To decrease unpeculiar coagulation, by polymerizing styrene and a compound shown by a certain general formula and also, styrene sulfonate under the existence of ultra fine silicic anhydride without an emulsifier, and sensitizing latex. CONSTITUTION:A 91g styrene, 0.66g sodium styreme sulfonate, 4.5g a compound represented formula A and 0.20g colloidal solution dispersed 0.06g ultra fine silicic anhydride in water, 0.05g potassium persulfate and 450g ion exchanged water, are polymerized at 70 deg.C for 24hr. Thus obtained latex is sensitized after incubation and a phosphoric acid buffer solution added an antiserum of a guinea pig already absorbed an antimanprotein antibody from the antiserum of immune guinea pig by a HBs antigen is added and sensitized latex is dispersed. False positivity appears very little when the intensity of cohesion for a man blood serum containing the HBs antigen by said reagent is measured.
JP5277981A 1981-04-07 1981-04-07 Latex reagent and measuring method for latex coagulation reaction Granted JPS57166559A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5277981A JPS57166559A (en) 1981-04-07 1981-04-07 Latex reagent and measuring method for latex coagulation reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5277981A JPS57166559A (en) 1981-04-07 1981-04-07 Latex reagent and measuring method for latex coagulation reaction

Publications (2)

Publication Number Publication Date
JPS57166559A true JPS57166559A (en) 1982-10-14
JPS6352708B2 JPS6352708B2 (en) 1988-10-19

Family

ID=12924333

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5277981A Granted JPS57166559A (en) 1981-04-07 1981-04-07 Latex reagent and measuring method for latex coagulation reaction

Country Status (1)

Country Link
JP (1) JPS57166559A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200315A (en) * 1990-07-25 1993-04-06 Eastman Kodak Company Particulate biologically active reagent containing polyoxyalkylene side chains, analytical element and methods for use of the reagent

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200315A (en) * 1990-07-25 1993-04-06 Eastman Kodak Company Particulate biologically active reagent containing polyoxyalkylene side chains, analytical element and methods for use of the reagent
US5330891A (en) * 1990-07-25 1994-07-19 Eastman Kodak Company Method for detection of a nucleic acid using particulate reagent having polyoxyalkylene side chains

Also Published As

Publication number Publication date
JPS6352708B2 (en) 1988-10-19

Similar Documents

Publication Publication Date Title
US4480042A (en) Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays
Slaght et al. An enzyme-linked immunosorbent assay (ELISA) for detecting chicken anti-reovirus antibody at high sensitivity
EP0302715B1 (en) Avidin- and biotin-immobilized reagents, analytical elements and methods of use
JPS5592693A (en) Determination of ligand
FR2370972A1 (en) Photometric assay of antibody and antigen - by measuring rate of extinction change on reaction with immunological partner bonded to polymer carrier particles
GB1358828A (en) Process for detecting the presence of human carcinoembryonic antigen material or component a or b thereof in blood
ES480964A1 (en) Process for detecting and quantifying an antigen or antibody.
CN107894509A (en) A kind of method for improving latex immunoturbidimetry antigen excess and the range of linearity
CN109633170A (en) A kind of Epstein-Barr virus shell antigen I gA antibody assay kit
Litchfield et al. Novel shell/core particles for automated turbidimetric immunoassays.
JP2892990B2 (en) Aqueous dispersion of biologically active polymer
GB1036591A (en) Immunological reagent for gonadotropins
JP2736143B2 (en) Immunochemical test agent containing carboxyl group-containing polymer
JPS57166559A (en) Latex reagent and measuring method for latex coagulation reaction
GB1497327A (en) Direct radioimmunoassay for hepatitis associated antigens and their antibodies
NO781473L (en) POLYMER MATRIX BASED ON ACRYLAMIDE.
SE7901166L (en) REAGENTS FOR USE IN IMMUNO ANALYSIS
JPS5759167A (en) Production of latex reagent
Sarjadi et al. A multiparametric approach to tumor markers detectable in serum in patients with carcinoma of the ovary or uterine cervix
JPS5523402A (en) Manufacture of reagent for indirect red corpuscle agglutination which absorbs simultaneously heterogenetic antibody
JPH0157688B2 (en)
CN105866439B (en) IgG kit based on latex immunoturbidimetry and preparation method thereof
Leek et al. Particle counting immunoassay (PACIA) V-its application to the determination of human placental lactogen
JPS5714610A (en) Production of latex for serodiagnotic reagent
Prince et al. Kinetic studies on the direct solid phase radioimmunoassay for hepatitis B antigen