JPS57166997A - Determination of quantity of microbial cell - Google Patents

Determination of quantity of microbial cell

Info

Publication number
JPS57166997A
JPS57166997A JP5361381A JP5361381A JPS57166997A JP S57166997 A JPS57166997 A JP S57166997A JP 5361381 A JP5361381 A JP 5361381A JP 5361381 A JP5361381 A JP 5361381A JP S57166997 A JPS57166997 A JP S57166997A
Authority
JP
Japan
Prior art keywords
medium
ethanol
concentration
saccharide
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP5361381A
Other languages
Japanese (ja)
Inventor
Wataru Okada
Hideki Fukuda
Hideki Nakatani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanegafuchi Chemical Industry Co Ltd
Original Assignee
Kanegafuchi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanegafuchi Chemical Industry Co Ltd filed Critical Kanegafuchi Chemical Industry Co Ltd
Priority to JP5361381A priority Critical patent/JPS57166997A/en
Priority to GB8209269A priority patent/GB2099453B/en
Priority to DE19823212447 priority patent/DE3212447A1/en
Publication of JPS57166997A publication Critical patent/JPS57166997A/en
Pending legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine the quantity of microbial cells, by culturing the microorganism in a medium containing a saccharide at a specific concentration range under aerobic conditions, and measuring the concentration of ethanol, or by collecting the above cultivation medium, adding said medium to a saccahride- containing medium charged in a separate cultivator, and measuring the produced ethanol.
CONSTITUTION: Microorganism capable of producing ethanol from saccharide is cultured in a cultivator 3, and a saccharide such as glucose, fructose, etc. is added to the cultivation liquid 8 at a concentration of 0.1W10g/l. Ethanol produced by the microorganism is sent to the detector with a carrier gas 6 through the "Teflon " tube 7, and its concentration is measured to determine the quantity of the microbial cells. As an alternative method, a proper amount of the above cultivation medium is added to the medium charged beforehand in a separate cultivator, and the amount of produced ethanol is measured. In this case, the concentration of saccharide in the medium prepared beforehand is adjusted to 0.1W50g/l.
COPYRIGHT: (C)1982,JPO&Japio
JP5361381A 1981-04-03 1981-04-08 Determination of quantity of microbial cell Pending JPS57166997A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP5361381A JPS57166997A (en) 1981-04-08 1981-04-08 Determination of quantity of microbial cell
GB8209269A GB2099453B (en) 1981-04-03 1982-03-30 A method for measuring the concentration of microorganisms
DE19823212447 DE3212447A1 (en) 1981-04-03 1982-04-02 METHOD FOR DETERMINING THE CONCENTRATION OF MICROORGANISMS

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5361381A JPS57166997A (en) 1981-04-08 1981-04-08 Determination of quantity of microbial cell

Publications (1)

Publication Number Publication Date
JPS57166997A true JPS57166997A (en) 1982-10-14

Family

ID=12947746

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5361381A Pending JPS57166997A (en) 1981-04-03 1981-04-08 Determination of quantity of microbial cell

Country Status (1)

Country Link
JP (1) JPS57166997A (en)

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