JPS5742634A - Latex sensitized with group polysaccharide antibody in streptococcus hemolyticus - Google Patents
Latex sensitized with group polysaccharide antibody in streptococcus hemolyticusInfo
- Publication number
- JPS5742634A JPS5742634A JP11703180A JP11703180A JPS5742634A JP S5742634 A JPS5742634 A JP S5742634A JP 11703180 A JP11703180 A JP 11703180A JP 11703180 A JP11703180 A JP 11703180A JP S5742634 A JPS5742634 A JP S5742634A
- Authority
- JP
- Japan
- Prior art keywords
- latex
- streptococcus hemolyticus
- hemolyticus
- group polysaccharide
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004816 latex Substances 0.000 title abstract 8
- 229920000126 latex Polymers 0.000 title abstract 8
- 241000193996 Streptococcus pyogenes Species 0.000 title abstract 6
- 150000004676 glycans Chemical class 0.000 title abstract 6
- 229920001282 polysaccharide Polymers 0.000 title abstract 6
- 239000005017 polysaccharide Substances 0.000 title abstract 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- 238000005345 coagulation Methods 0.000 abstract 2
- 230000015271 coagulation Effects 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 229920002307 Dextran Polymers 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- 239000004793 Polystyrene Substances 0.000 abstract 1
- 239000000654 additive Substances 0.000 abstract 1
- 238000013399 early diagnosis Methods 0.000 abstract 1
- 230000005484 gravity Effects 0.000 abstract 1
- 230000003053 immunization Effects 0.000 abstract 1
- 238000002649 immunization Methods 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 229920002223 polystyrene Polymers 0.000 abstract 1
- 230000001235 sensitizing effect Effects 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000003381 stabilizer Substances 0.000 abstract 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PURPOSE: The titled latex that is made by sensitizing latex with the group polysaccharide antibody against Streptococcus hemolyticus that is prepared by isolating and purifying from the cell antibody of Streptococcus hemolyticus, thus enabling the early diagnosis of infections caused by Streptococcus hemolyticus by the coagulation reaction of reversed passive latex.
CONSTITUTION: The group polysaccharide that is contained in all the strains of Streptococcus hemolyticus is mixed with the cell antibody of ttreptococcus hemolyticus made by immunization to form the precipitant of only the immune complex, which is centrifuged for separation. Then, the precipitant is thoroughly washed to produce pure polysaccharide immune complex. The complex is dissociated to form the group polysaccharide antibody against Streptococcus hemolyticus, which is sensitized to latex particles (preferably polystyrene of 0.01W10μ average particle size and of 0.9W1.4 specifi gravity). The sensitized latex is used to detect the group polysaccharide antibody by the reversed passive latex coagulation reaction. Further addition of glycine, dextran and other additives to the latex is preferred as stabilizers.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11703180A JPS5742634A (en) | 1980-08-27 | 1980-08-27 | Latex sensitized with group polysaccharide antibody in streptococcus hemolyticus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11703180A JPS5742634A (en) | 1980-08-27 | 1980-08-27 | Latex sensitized with group polysaccharide antibody in streptococcus hemolyticus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5742634A true JPS5742634A (en) | 1982-03-10 |
Family
ID=14701728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11703180A Pending JPS5742634A (en) | 1980-08-27 | 1980-08-27 | Latex sensitized with group polysaccharide antibody in streptococcus hemolyticus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5742634A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60164250A (en) * | 1984-01-27 | 1985-08-27 | メリデイアン・ダイアグノステイツクス・インコ−ポレ−テツド | Method of detecting beta hemolytic streptococcus antigen |
| JPS60188847A (en) * | 1984-02-27 | 1985-09-26 | ベクトン・デイツキンソン・アンド・カンパニ− | Method of diagnosing streptococcus a |
| US5196239A (en) * | 1988-12-17 | 1993-03-23 | Bayer Aktiengesellschaft | Process for uniform distribution of a free-flowing reaction mixture onto a continuously transported substrate |
-
1980
- 1980-08-27 JP JP11703180A patent/JPS5742634A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60164250A (en) * | 1984-01-27 | 1985-08-27 | メリデイアン・ダイアグノステイツクス・インコ−ポレ−テツド | Method of detecting beta hemolytic streptococcus antigen |
| JPS60188847A (en) * | 1984-02-27 | 1985-09-26 | ベクトン・デイツキンソン・アンド・カンパニ− | Method of diagnosing streptococcus a |
| US5196239A (en) * | 1988-12-17 | 1993-03-23 | Bayer Aktiengesellschaft | Process for uniform distribution of a free-flowing reaction mixture onto a continuously transported substrate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Burnet et al. | The" T" Antigen of Guinea-Pig and Human Red Cells. | |
| CA982065A (en) | Process and apparatus for the separation of gas mixtures into component fractions according to their molecular or atomic weight | |
| JPS5263178A (en) | Gas separation unit | |
| Wofsy et al. | Cell separation on affinity columns: the preparation of pure populations of anti-hapten specific lymphocytes | |
| Lightsey Jr et al. | Platelet-associated immunoglobulin G in childhood idiopathic thrombocytopenic purpura | |
| Moss | Drug‐induced immune thrombocytopenia | |
| JPS5742634A (en) | Latex sensitized with group polysaccharide antibody in streptococcus hemolyticus | |
| GB8929297D0 (en) | Method of separation | |
| SE8205891L (en) | ALFA-L ANTIBODY AND SET FOR PRODUCING THEREOF | |
| Levine et al. | Carbonyl iron: a new adjuvant for experimental autoimmune diseases | |
| JPS57197471A (en) | Blood coagulation accelerant | |
| Schmidt et al. | Demonstration of rubella complement-fixing antigens of two distinct particle sizes by gel filtration on sephadex G-200. | |
| JPS5653465A (en) | Improved method in measurement of enzyme immunity | |
| JPS52882A (en) | Suspension polymerization of vinyl chloride | |
| JPS524587A (en) | Preparation of vinylidene chloride copolymer | |
| Holtzer | Experimental delayed type allergy without demonstrable antibodies: II. In vitro activity of reticulo-endothelial cells | |
| JPS5257667A (en) | Dust proof hopper | |
| Thomson et al. | Bacterial immunoabsorbent columns | |
| JPS55136230A (en) | Selective specific cell adsorbent | |
| IKEMOTO et al. | Immunogenetical Studies on the AB Type with Anti-A in its Serum. I Immunological and Immunochemical Characterization of the AB (A'Bα) Type Red Cells | |
| Delmas-Marsalet et al. | Study of Two Anti-β-Lipoprotein Isoantibodies Detected in Multitransfused Patients | |
| Popovic et al. | Antigenic similarity between Yersinia enterocolitica and other gram negative bacteria. | |
| JPS52111069A (en) | Dust removing method | |
| JPS52136896A (en) | Zeolite adsorbents | |
| JPS5660605A (en) | Treatment of waste water containing emulsified oil |