JPS5771399A - Analytical method of minor constituent in specimen and apparatus - Google Patents
Analytical method of minor constituent in specimen and apparatusInfo
- Publication number
- JPS5771399A JPS5771399A JP14635480A JP14635480A JPS5771399A JP S5771399 A JPS5771399 A JP S5771399A JP 14635480 A JP14635480 A JP 14635480A JP 14635480 A JP14635480 A JP 14635480A JP S5771399 A JPS5771399 A JP S5771399A
- Authority
- JP
- Japan
- Prior art keywords
- specimen
- light
- luminol
- peroxidase
- shutter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000470 constituent Substances 0.000 title abstract 2
- 238000004458 analytical method Methods 0.000 title 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract 4
- 102000003992 Peroxidases Human genes 0.000 abstract 2
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 abstract 2
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 108010015776 Glucose oxidase Proteins 0.000 abstract 1
- 239000004366 Glucose oxidase Substances 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 229940116332 glucose oxidase Drugs 0.000 abstract 1
- 235000019420 glucose oxidase Nutrition 0.000 abstract 1
- 150000002978 peroxides Chemical class 0.000 abstract 1
- 238000004445 quantitative analysis Methods 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To analyze minor constituents in a specimen in a short time with a high accuracy, by measuring the quantity of light emitted by adding a luminous light emitting reagent to hydrogen peroxide generated due to the action of an enzyme on the specimen.
CONSTITUTION: The quantitative analysis is carried out by sensing the light emitted in the presence of luminol and a peroxidase from hydrgen peroxide generated by the action of an oxidase on a specimen, e.g. in the determination of glucose by a glucose oxidase, with a photomultiplier tube 24, etc. A sample prepared by adding the luminol and peroxidase to the specimen containing hydrogen peroxide is injected into a vial 14 and contained in an accommodating part 12 in a dark box 10, and a shutter 18 is closed. A shutter 26 is then opened, and light passes through a transparnt plate 20 and enters the photomultiplier tube 24. The light is then counted as electric signals by an amplifying and counting circuit 28 and then indicated in a display part 30.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14635480A JPS5937946B2 (en) | 1980-10-21 | 1980-10-21 | Trace component analyzer in specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14635480A JPS5937946B2 (en) | 1980-10-21 | 1980-10-21 | Trace component analyzer in specimen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5771399A true JPS5771399A (en) | 1982-05-04 |
| JPS5937946B2 JPS5937946B2 (en) | 1984-09-12 |
Family
ID=15405812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14635480A Expired JPS5937946B2 (en) | 1980-10-21 | 1980-10-21 | Trace component analyzer in specimen |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5937946B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60135750A (en) * | 1983-12-23 | 1985-07-19 | Agency Of Ind Science & Technol | Chemical emission type analytical device for trace component in liquid |
| JPS6123968A (en) * | 1984-07-11 | 1986-02-01 | Jeol Ltd | Chromatograph detection |
| US5084381A (en) * | 1987-03-09 | 1992-01-28 | Suntory Limited | Assay method for detecting hydrogen peroxide |
| JPH04343217A (en) * | 1991-05-20 | 1992-11-30 | Tokico Ltd | Automatic liquid control device |
| JPH0593692A (en) * | 1991-05-16 | 1993-04-16 | Dam Suigenchi Kankyo Seibi Center | Phosphate ion concentration measuring device |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9707143B2 (en) | 2012-08-11 | 2017-07-18 | Hill-Rom Services, Inc. | Person support apparatus power drive system |
-
1980
- 1980-10-21 JP JP14635480A patent/JPS5937946B2/en not_active Expired
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60135750A (en) * | 1983-12-23 | 1985-07-19 | Agency Of Ind Science & Technol | Chemical emission type analytical device for trace component in liquid |
| JPS6123968A (en) * | 1984-07-11 | 1986-02-01 | Jeol Ltd | Chromatograph detection |
| US5084381A (en) * | 1987-03-09 | 1992-01-28 | Suntory Limited | Assay method for detecting hydrogen peroxide |
| JPH0593692A (en) * | 1991-05-16 | 1993-04-16 | Dam Suigenchi Kankyo Seibi Center | Phosphate ion concentration measuring device |
| JPH04343217A (en) * | 1991-05-20 | 1992-11-30 | Tokico Ltd | Automatic liquid control device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5937946B2 (en) | 1984-09-12 |
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