JPS5771400A - Analytical method of minor constituent in specimen and apparatus - Google Patents
Analytical method of minor constituent in specimen and apparatusInfo
- Publication number
- JPS5771400A JPS5771400A JP14635580A JP14635580A JPS5771400A JP S5771400 A JPS5771400 A JP S5771400A JP 14635580 A JP14635580 A JP 14635580A JP 14635580 A JP14635580 A JP 14635580A JP S5771400 A JPS5771400 A JP S5771400A
- Authority
- JP
- Japan
- Prior art keywords
- specimen
- light
- peroxidase
- luminous
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000470 constituent Substances 0.000 title abstract 2
- 238000004458 analytical method Methods 0.000 title 1
- 102000003992 Peroxidases Human genes 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract 2
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To analyze minor constituents in a specimen in a short time with a high accuracy, by adding a luminous reagent capable of emitting light on reaction with a peroxidase to the specimen to emit the light.
CONSTITUTION: A luminous (light emitting) reagent consisting of luminol and hydrogen peroxide is added to a specimen containing a peroxidase to emit light, and the peroxidase is determined by the quantity of the emitted light. A vial 14 containing a sample prepared by adding the luminous (light emitting) reagent to the specimen is accommodated in a dark box 10, and a shutter 18 is closed to open a shutter 26. The light emitted in the vial 14 passes through a transparent plate 20 and enters a photomultiplier tube 24, and the resultant electric signals are counted by an amplifying and counting circuit 28 for a time set by a timer 32 and indicated in a display part 30.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14635580A JPS594999B2 (en) | 1980-10-21 | 1980-10-21 | Method and device for analyzing trace components in samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14635580A JPS594999B2 (en) | 1980-10-21 | 1980-10-21 | Method and device for analyzing trace components in samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5771400A true JPS5771400A (en) | 1982-05-04 |
| JPS594999B2 JPS594999B2 (en) | 1984-02-02 |
Family
ID=15405834
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14635580A Expired JPS594999B2 (en) | 1980-10-21 | 1980-10-21 | Method and device for analyzing trace components in samples |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS594999B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62220865A (en) * | 1986-03-24 | 1987-09-29 | Yatoron:Kk | Immunological measurement mthod for homogeneous system enzyme |
| JP2012198242A (en) * | 2012-06-21 | 2012-10-18 | Hitachi Ltd | Chemiluminescence measuring device |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9027131D0 (en) * | 1990-12-14 | 1991-02-06 | Rice Evans Catherine | Diagnostic test |
| JPH0564700U (en) * | 1992-02-04 | 1993-08-27 | 大同特殊鋼株式会社 | Slag flow monitoring device at the outlet |
-
1980
- 1980-10-21 JP JP14635580A patent/JPS594999B2/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62220865A (en) * | 1986-03-24 | 1987-09-29 | Yatoron:Kk | Immunological measurement mthod for homogeneous system enzyme |
| JP2012198242A (en) * | 2012-06-21 | 2012-10-18 | Hitachi Ltd | Chemiluminescence measuring device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS594999B2 (en) | 1984-02-02 |
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