JPS5779898A - Preparation of human-type insulin - Google Patents
Preparation of human-type insulinInfo
- Publication number
- JPS5779898A JPS5779898A JP55154620A JP15462080A JPS5779898A JP S5779898 A JPS5779898 A JP S5779898A JP 55154620 A JP55154620 A JP 55154620A JP 15462080 A JP15462080 A JP 15462080A JP S5779898 A JPS5779898 A JP S5779898A
- Authority
- JP
- Japan
- Prior art keywords
- insulin
- desdipeptide
- swine
- enzyme
- type insulin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 11
- 229940125396 insulin Drugs 0.000 title abstract 7
- 102000004877 Insulin Human genes 0.000 title abstract 4
- 108090001061 Insulin Proteins 0.000 title abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 4
- 108090000790 Enzymes Proteins 0.000 abstract 4
- 102000035195 Peptidases Human genes 0.000 abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 2
- 239000012736 aqueous medium Substances 0.000 abstract 2
- 235000019833 protease Nutrition 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000589565 Flavobacterium Species 0.000 abstract 1
- 235000019687 Lamb Nutrition 0.000 abstract 1
- 125000003277 amino group Chemical group 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 239000000376 reactant Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: To prepare the titled substance, easily, in high efficiency, by breaking the peptide bonds of swine-type insulin with an enzyme acting specifically to the bond, and forming peptide bonds using the same enzyme.
CONSTITUTION: An enzyme acting specifically to the Pro-Lys bond in the B- chain of swine-type insulin, e.g. peptidase produced by the bacteria belonging to Flavobacterium genus or peptidase separated from the kidney of a lamb, is added to an aqueous medium. Swine-type insulin is hydrolyzed in the aqueous medium to obtain desdipeptide-B29,B30-insulin. The amino group of the desdipeptide-B29,B30-insulin is protected to obtain X2-desdipeptide-B29,B30-insulin, which is condensed with N -X-Lys-Thr in the presence of the above enzyme. The reaction is carried out by using the latter reactant in excess to the former one, and rapidly removing the reaction product from the reaction system.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55154620A JPS5779898A (en) | 1980-11-05 | 1980-11-05 | Preparation of human-type insulin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55154620A JPS5779898A (en) | 1980-11-05 | 1980-11-05 | Preparation of human-type insulin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5779898A true JPS5779898A (en) | 1982-05-19 |
| JPH0320237B2 JPH0320237B2 (en) | 1991-03-18 |
Family
ID=15588154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55154620A Granted JPS5779898A (en) | 1980-11-05 | 1980-11-05 | Preparation of human-type insulin |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5779898A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0087238A1 (en) * | 1982-02-08 | 1983-08-31 | Biogen N.V. | Am improved method for preparing human insulin from non-human insulin |
| CN110305819A (en) * | 2019-08-16 | 2019-10-08 | 南京工业大学 | A highly efficient feather-degrading bacterial strain and its application |
-
1980
- 1980-11-05 JP JP55154620A patent/JPS5779898A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0087238A1 (en) * | 1982-02-08 | 1983-08-31 | Biogen N.V. | Am improved method for preparing human insulin from non-human insulin |
| CN110305819A (en) * | 2019-08-16 | 2019-10-08 | 南京工业大学 | A highly efficient feather-degrading bacterial strain and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0320237B2 (en) | 1991-03-18 |
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