JPS585185A - Medium for cultivating fruit body of basidiomycetes - Google Patents

Abstract

PURPOSE:A medium for cultivating fruit bodies of Basidiomycetes capable of producing fruit bodies in high field, by using crushed pods of peanuts. CONSTITUTION:A medium comprising powdered, crushed, or thinly cut woody raw material, rice bran, and/or maize bran is uniformly blended with 5-28wt%, preferably 7-12wt% calculated as dry weight based on total medium amount of pods of peanuts which are crushed into preferably 4-14 meshes, preferably 8-12 meshes.

Description

[Detailed description of the invention] The present invention relates to a medium for cultivating basidiomycete fruiting bodies.

Traditionally, edible mushrooms such as nameko, enokitake, and oyster mushrooms have been obtained by inoculating and culturing the inoculum of these edible mushrooms in a medium containing sawdust as the main ingredient and, if necessary, an appropriate amount of nutrients such as rice bran. .

However, due to the increasing demand for edible mushrooms, there is a shortage of high-quality sawdust (hardwoods are especially suitable among hardwoods and coniferous trees) that can be used as a raw material for the above-mentioned culture medium, and the price of sawdust is soaring. The reality is that it is extremely difficult to obtain.

On the other hand, while peanut seeds are edible, the reality is that the pods have no useful use and are incinerated.

Therefore, as a result of various studies in view of the above-mentioned circumstances, the present inventors inoculated and cultured basidiomycetes such as nameko and shiitake mushrooms into a medium containing sawdust and rice bran and/or corn bran mixed with crushed peanuts. The present invention was completed based on the findings that fruit bodies can be obtained in high yield and the amount of sawdust added can be significantly reduced.

That is, the present invention is a crushed ground peanut and a crushed and split culture medium.

The present invention will be explained in detail below.

First, the raw material used in the present invention may be any variety of fallen flowers.

In addition, the peanuts may be used as they are (unheated and roasted) or may be subjected to any treatment such as heated and roasted in a conventional manner.

Then, the fallen flower is separated into pods and seeds by a conventional method to obtain the pods.

The pods are then crushed.

The degree of crushing of the pods may be of any particle size, for example, j~
/≠ mesh, preferably t~/2 mesh.

In addition, when the degree of pulverization exceeds ≠ mesh and/
If it is less than ≠Metsu, the formation of basidiomycete fruiting bodies takes an extremely long time, which is not preferable.

Any means may be used as the crushing means, and examples thereof include methods using an evaporator, hammer mill, cutter mill, rotary crusher, mill, and the like.

Next, the ground peanut manure obtained in this way can be homogeneously mixed with wood raw materials that have been ground, split, or sliced, and rice bran and/or corn bran, and used as a medium for cultivating basidiomycete fruiting bodies. Furthermore, if necessary,
Water: Nitrogen sources such as cornstap liquor, wrinkles, amino acids, soybean meal, soy sauce lees; Carbon sources such as glucose and maltose; Minerals such as potassium phosphate, lime carbonate, magnesium sulfate; Vitamins, etc. It is also possible to use a combination of seven or more types.

The wood material used in the present invention may be of any tree species as long as it is normally used for cultivating basidiomycetes, such as beech, Quercus serrata, Quercus oak, Japanese birch, Japanese oak, Japanese oak, Japanese oak, Japanese oak, poplar, and alder. , oak, chinquapin, tab, teak, and other woody materials.

The wood raw material is crushed or crushed using a conventional crusher or crusher, or it is made into flakes using a plane, a slicer, or the like. At this time, the particle size when crushed or crushed is approximately ≠ mesh, which is the preferred size? Preferably, the particles are finer than mesh, and the flakes have a thickness of about 3 tnm or less, preferably under the shell plate.

In addition, the blending ratio of crushed peanut excrement may be any violet, for example, the proportion of groundnut excrement dry weight is j~ per total medium weight.
, 2? It is desirable to mix the amount so that the amount is 7 to 2, preferably 7 to 2.

Furthermore, the blending ratio of rice bran to corn bran may be any ratio.

Next, the above-mentioned basidiomycete fruiting body cultivation medium is placed in a suitable container, subjected to a normal sterilization treatment, and then inoculated with a basidiomycete inoculum, and then cultured according to a normal culture method.
Obtain basidiomycete fruiting bodies.

As the basidiomycete that can be applied to the present invention, any type of basidiomycete may be used as long as it belongs to the genus Sugitake, Shiitake, Enokitake, Oyster mushroom, Maitake, etc. Among them, Nameko, Shiitake, and Enokitake can be used. , ε-ratake, maitake, etc. are particularly suitable.

As described above, the present invention makes it possible to obtain basidiomycete fruiting bodies in high yield, and in addition, the present invention will be described in detail with reference to Examples.

Example 1 Beech sawdust 3109 (dry weight), corn bran t
0? (dry weight), water, gl(W
/W), and then add water to this to give a water content of 63% (
W/W ) crushed peanut manure (pulverized particle size/θ mesh) was added to the mixture at a concentration of 9% (dry weight %).

The hydrate obtained in this way was filled into a cultivation bag and sealed in a conventional manner, and the pressure/Ky/crA (gauge pressure)
After sterilizing the cells by applying saturated steam at temperature/2θ°C for 9θ minutes, the cells were cooled to room temperature to obtain a culture medium (the present invention).

In addition, the control for comparison is beech sawdust 310? (dry weight) and corn bran θf (dry weight), add water to 1% (W/W), put the obtained product in a cultivation bag, and then prepare according to the present invention. It was obtained by heat sterilization in exactly the same manner as the method.

Next, nameko inoculum was inoculated into each of the above-mentioned media, and the temperature was increased to 23°C.
The cells were cultured for 3 months at a temperature of 60 to 70% humidity, and then transferred to a fruiting body generation chamber at a temperature of /j°C and a humidity of -tθ to 9Q.
Table 1 shows the yield obtained by developing fruiting bodies for λ months under the conditions described above.

As is clear from Table 1, the fruiting body yield of the present invention is significantly increased compared to the control.

Example 2 To a mixture of 3/θt (dry weight) of beech sawdust and 10 g (dry weight) of rice bran, water was added to make the water content 3% (W/W). Pulverized peanut pods (pulverized particle size/θ mesh) of W/W) were added at a concentration of 9 g (dry weight %).

The thus obtained hydrate was filled into a cultivation bag, sterilized by applying saturated steam at temperature/2θ° C. for 9θ minutes under pressure), and then cooled to room temperature to obtain a culture medium.

The control medium for comparison was a mixture of beech sawdust 3/θf (dry weight) and rice bran tOf (dry weight), water was added to give a moisture content of 4j% (W/W), and the rest was prepared using the method of the present invention. It was obtained by heat sterilization in exactly the same manner as the culture medium preparation method.

Next, each of the above-mentioned media was inoculated with shiitake seed fungi, and the temperature and
Cultured at 23°C and humidity of 60 to 7θ,
Afterwards, it is transferred to the fruiting body generation chamber, at a temperature of 1J-℃ and humidity? θ〜
Table 2 shows the yield obtained after fruiting bodies were allowed to develop for several months under 90% conditions.

As is clear from Table 2, the fruiting body yield of the present invention is significantly increased compared to the control.

Example 3 Beech sawdust 3101 (dry weight) and corn bran≠2
1 (dry weight) and rice bran/2v (dry weight), water was added to give a moisture content of 3% (W/W), and further water was added to this mixture, <J% (, W/W). ) of ground peanut manure (pulverized particle size lθ mesh) was added to 9% (dry weight %).
) was added.

The thus obtained hydrate lK9 was filled into a cultivation bag, which was sealed in a conventional manner, and the bag was sterilized by applying saturated steam at a temperature of 12θ°C for 90 minutes at a pressure of /Ks+/c1A (gauge pressure). A medium was obtained by cooling to room temperature. (The present invention) The control medium for comparison was prepared by adding 310 ftC of beech sawdust (dry weight) and corn bran to 11% of the method for preparing the medium of the present invention. jr9 (dry weight) and rice bran/2f (dry weight), with a moisture content of j3% (W/W).
It was obtained in exactly the same manner as the method for preparing the culture medium of the present invention, except that water was added as shown below.

Next, nameko inoculum was inoculated into each of the above-mentioned media, and the temperature was increased to 23°C.
℃ for 3 months at a humidity of 60 to 70%, then transferred to a fruiting body generation chamber and incubated at a temperature of 3.
As is clear from the table above, it can be seen that the fruiting body yield of the present invention is significantly increased compared to the control.

Claims (1)

[Claims] A culture medium for cultivating basidiomycete fruiting bodies homogeneously containing crushed ground peanuts, crushed, crushed or thinly sliced wood material, and rice bran and/or corn bran.