JPS5897341A - Blood sampling pipe - Google Patents
Blood sampling pipeInfo
- Publication number
- JPS5897341A JPS5897341A JP56196307A JP19630781A JPS5897341A JP S5897341 A JPS5897341 A JP S5897341A JP 56196307 A JP56196307 A JP 56196307A JP 19630781 A JP19630781 A JP 19630781A JP S5897341 A JPS5897341 A JP S5897341A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- coating
- blood collection
- water
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- External Artificial Organs (AREA)
- Materials For Medical Uses (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
■６発明の背景
技術分野
本発明は、血液を採取し、遠心分離によシ血液を血清相
と血餅相とに分離するために使用される改良された採血
管に関する。Detailed Description of the Invention ■6 Background of the Invention Technical Field The present invention relates to an improved blood collection tube used for collecting blood and separating the blood into a serum phase and a blood clot phase by centrifugation. Regarding.
先行技術
従来、血液検査を目的とする血液の採取器具として、採
血管が一般的に使用されている。この採血管は、通常ゴ
ム栓体によって密閉され内部が一定真空度に保たれたガ
ラス製試験管であシ、管内の真空度によシ採血針を通し
て所定量の血液を定量的に採取できるものである。また
、検査目的に応じて抗凝固剤、解糖阻止剤等を予じめ入
れて置くこともできる為、検査前の煩雑な準備操作が不
要であると共に採血後、検査までの間密封状態下にある
ので検体の汚染の恐れもない。したがって１採血後その
まま検査に供する試験管として有用な採血管である。BACKGROUND OF THE INVENTION Conventionally, blood collection tubes have been commonly used as instruments for collecting blood for the purpose of blood tests. This blood collection tube is usually a glass test tube that is sealed with a rubber stopper and kept at a certain degree of vacuum inside, and can quantitatively collect a predetermined amount of blood through a blood collection needle depending on the degree of vacuum inside the tube. It is. In addition, anticoagulants, glycolytic inhibitors, etc. can be added in advance depending on the purpose of the test, so there is no need for complicated preparations before the test, and the blood is kept in a sealed state after blood collection until the test. , so there is no risk of sample contamination. Therefore, this blood collection tube is useful as a test tube that can be used directly for testing after blood collection.
上述の採血管の中でも、血清学的検査及び生化学検査等
の血清を検体とした検査を目的としたものは検体となる
血清を血餅と明確に分離することが必要であることから
、血清帯域における採血管内面への血餅の付着を防ぐた
めガラス製試験管の内面を清浄にしておかねばならない
。しかし、ガラス製試験管内面は極めて清浄にしておい
ても、ゴム栓体に含まれる微量の水分によってガラス面
が経時的に侵蝕されたシイム栓体からの微量の揮発物が
ガラス面に吸着さたシ、また血清分離用の分離剤を管底
に収容する採血管にあっては該分離剤からの揮発物がガ
ラス面に吸着されることがある為、それら不純物を核と
してフィブリン又は血餅が付着し、遠心分離後にそれら
が血清帯域に残ることがあった。Among the above-mentioned blood collection tubes, those intended for tests using serum as a sample such as serological tests and biochemical tests require that the serum sample be clearly separated from blood clots. The inner surface of the glass test tube must be kept clean to prevent blood clots from adhering to the inner surface of the blood collection tube in the zone. However, even if the inner surface of a glass test tube is kept extremely clean, trace amounts of volatile matter from the shim stopper, whose glass surface has been eroded over time by trace amounts of moisture contained in the rubber stopper, will be adsorbed onto the glass surface. In addition, in blood collection tubes that contain a separating agent for serum separation at the bottom of the tube, volatile substances from the separating agent may be adsorbed to the glass surface, so these impurities may form fibrin or blood clots. were attached and they could remain in the serum zone after centrifugation.
そこで、ガラス面にシリコーン被膜を施し、ガラス面へ
の直接の揮発性不純物の付着を防止して揮発性不純物を
核とするフィブリン又は血餅の付着を防止するようにし
たものがある。ところが、このシリコーンは血液の凝固
を遅延させる働きがｂす、ｒ線による滅菌及び経時゛変
化によりシリコーンが徐々に分解・配向を進めて表面状
態を悪化させる為、ますます凝固が遅延することになる
。Therefore, there are devices in which a silicone coating is applied to the glass surface to prevent volatile impurities from directly adhering to the glass surface, thereby preventing fibrin or blood clots containing volatile impurities from adhering to the glass surface. However, this silicone has the effect of delaying blood coagulation, and due to R-ray sterilization and changes over time, silicone gradually decomposes and orients itself, worsening the surface condition, which further delays coagulation. Become.
この為、遠心分離後に血餅相の上方にフィブリン血清の
採取量は減少することになる。また血清内に浮遊混入し
たフィブリンを血餅相へ移動させる為に遠心力を増大す
ると血球破壊を招来するので有効かつ明確な血清と血餅
の分離は期待できない。For this reason, the amount of fibrin serum collected above the blood clot phase after centrifugation will decrease. Furthermore, increasing the centrifugal force to move fibrin suspended in the serum to the clot phase will lead to destruction of blood cells, so effective and clear separation of the serum and clot cannot be expected.
ま几、血液凝固の遅延を防止するため、ガラス粉末を混
入した水溶性物質の被膜をガラス管内面に形成し、ガラ
ス管内へ血液を導入する際に、当該水溶性物質被膜の溶
解によシガラス粉末を血液中に分散°させるようにした
ものが知られている（米国特許第４１５３７３９号）。In order to prevent delays in blood coagulation, a coating of water-soluble substance mixed with glass powder is formed on the inner surface of the glass tube, and when blood is introduced into the glass tube, the coating of water-soluble substance is dissolved. A device in which the powder is dispersed in blood is known (US Pat. No. 4,153,739).
しかし、ガラス粉末の混入によシ前記被膜をゴム栓の嵌
合部にまで形成すれば、ゴム栓体の嵌着が困難となり、
また密封性を損なう虞があるのでガラス管内面の全域を
被うことはできない。したがって１ゴム栓体の嵌合部に
は前記被膜が形成されず、ガラス面が露出しておシ、そ
の為ゴム栓体を嵌着してもゴム栓体の端部と前記被膜端
部との間にガラス面が露出するのを避けることができな
い。よってその部分において血餅の付着等の不都合が生
じる。また、ゴム栓体の表面は微視的には平滑ではなく
多くの凹凸がある為、この部分にもフィブリン）血餅等
が付着し遠心分離後も付着したままとなシ、脱枠時の栓
体と採血管壁との摩擦等にょシ剥離し血清相中に混入し
検査値に悪影響を及ぼすことがあったＯ
■０発明の目的
本発明は、試験管内での血液の凝固が速やかでかつ管内
壁及びゴム栓体表面へのフィブリンや血餅等の付着がな
く、安定かつ確実な血清と血餅の分離が期待できる採血
管を提供し、血清学的検査及び生化学検査の信頼性を向
上させることを目的とするものである。However, if the coating is formed on the fitting part of the rubber stopper due to the contamination of glass powder, it becomes difficult to fit the rubber stopper body.
Furthermore, the entire inner surface of the glass tube cannot be covered because there is a risk of impairing the sealing performance. Therefore, the coating is not formed on the fitting part of the rubber stopper 1, and the glass surface is exposed. Therefore, even when the rubber stopper is fitted, the end of the rubber stopper and the end of the coating are exposed. It is unavoidable that the glass surface will be exposed during this period. Therefore, problems such as adhesion of blood clots occur in that area. In addition, the surface of the rubber stopper is not microscopically smooth and has many irregularities, so fibrin, blood clots, etc. may adhere to this area and remain attached even after centrifugation. Due to friction between the stopper and the wall of the blood collection tube, the stopper may peel off and be mixed into the serum phase, adversely affecting test values.O Purpose of the InventionThe present invention is designed to rapidly coagulate blood in a test tube. We also provide blood collection tubes that do not have fibrin or blood clots attached to the inner wall of the tube or the surface of the rubber stopper, and can be expected to perform stable and reliable separation of serum and blood clots, thereby increasing the reliability of serological and biochemical tests. The purpose is to improve the
上記目的はつぎのような本発明による採血管によって達
成される。すなわち、この採血管は管状容器と、該容器
開口部に着脱可能でかつ密封可能に嵌着された弾性を有
するゴム栓体とを有する採血管において、該栓体の該容
器側端面および該容仝内面との密着面に水溶性シリコー
ンの被膜を形成するとともに、前記容器内面全域には水
溶性高分子の被膜を形成したことを特徴とする採血管で
ある。The above object is achieved by the blood collection tube according to the present invention as follows. That is, this blood collection tube has a tubular container and an elastic rubber stopper that is removably and sealably fitted into the opening of the container, and the end surface of the stopper on the side of the container and the container are connected to each other. This blood collection tube is characterized in that a water-soluble silicone coating is formed on the surface that is in close contact with the inner surface of the container, and a water-soluble polymer coating is formed on the entire inner surface of the container.
さらに本発明は上記水溶性高分子をポリビニルピロリド
ンとしたものである。ポリビニルピロリドンは水溶性に
富み、血漿増量剤としても使用されているものであシ、
血液に対し不活性であシ経時変化も少ない。さらに血液
に接触したときの溶解性に優れ特に好ましい。また、上
記ポリビニルビｏ！ＪトンｔＤ被膜ヲ１． Ｏｘ １０
−３１ｎ９／ＣＷＬ２以上とすルコとによシ採血管内面
にばらつきのない均一な被膜を形成することができる。Furthermore, the present invention uses polyvinylpyrrolidone as the water-soluble polymer. Polyvinylpyrrolidone is highly water-soluble and is also used as a plasma expander.
It is inert to blood and changes little over time. Furthermore, it is particularly preferable because it has excellent solubility when it comes into contact with blood. In addition, the above polyvinyl vinyl o! JtontD coating 1. Ox10
-31n9/CWL2 or more makes it possible to form a uniform coating on the inner surface of the blood collection tube.
■０発明の詳細な説明
以下本発明の構成を図面に示す実施例に基づいて詳細に
説明する。(2) Detailed Description of the Invention The configuration of the present invention will be described in detail below based on embodiments shown in the drawings.
水溶性高分子の被膜は、管状容器すなわちガラス管１の
内面全域に形成されている。該被膜２の形成は十分に清
浄なガラス管１に水溶性高分子水溶液を噴霧し、あるい
は、ガラス管１を水溶性高分子水溶液中に浸漬し、次い
で乾燥すること等によシ行う。水溶性高分子としては、
ガラス管１の内面を被覆する膜を形成でき、かつ、該ガ
ラス管内への血液の導入の際に容易に血液中に溶解する
ものであれば使用可能であるが、毒性、検査値への影響
等を考慮すると、ポリビニルピロリドン（ＰＶＰ）、メ
チルセルロース（ＭＣ） 、カルがキシメチルセルロー
ス（ＣＭＣ） 、ヒドロキシプロピルメチルセルロース
（ＨＰＭＣ）、ポリビニルアルコール（ｐＶＡ）、デキ
ストランおよびポリエチレングリコール等が望ましい。A water-soluble polymer coating is formed over the entire inner surface of the tubular container, that is, the glass tube 1. The coating 2 is formed by spraying an aqueous solution of a water-soluble polymer onto a sufficiently clean glass tube 1, or by immersing the glass tube 1 in an aqueous solution of a water-soluble polymer and then drying. As a water-soluble polymer,
It can be used as long as it can form a film that coats the inner surface of the glass tube 1 and is easily dissolved in blood when blood is introduced into the glass tube, but it may have an impact on toxicity and test values. Considering these factors, polyvinylpyrrolidone (PVP), methylcellulose (MC), carboxymethylcellulose (CMC), hydroxypropylmethylcellulose (HPMC), polyvinyl alcohol (pVA), dextran, polyethylene glycol, etc. are desirable.
特にＰｖＰは水溶性に富み血漿増量剤としても使用され
ているものであり血液に対して不活性であシ、経時変化
も少ない。さらに採血管内に流入した血液と接触したと
きの溶解性に優れ、清浄なガラス管内面を早く露出させ
る為、親水性のガラス表面が有する血液凝固作用を妨げ
ることがない。該ＰｖＰの被膜は” ０×” ０−’
”９７ａｍ２以上で形成することによシ、よシ均一な被
膜とすることができる。該水溶性高分子の被膜２が施さ
れたガラス管１には、弾性を有するゴム栓体３が嵌着さ
れ、管内の減圧状態を保ち得るように設けられている。In particular, PvP is highly water-soluble and is also used as a plasma expander, is inert to blood, and changes little over time. Furthermore, it has excellent solubility when it comes into contact with blood flowing into the blood collection tube, and because the clean inner surface of the glass tube is quickly exposed, it does not interfere with the blood coagulation effect of the hydrophilic glass surface. The PvP coating is "0x"0-'
A more uniform coating can be obtained by forming the coating with a thickness of 97 am2 or more.An elastic rubber stopper 3 is fitted into the glass tube 1 coated with the water-soluble polymer coating 2. It is installed to maintain a reduced pressure inside the pipe.
ゴム栓体の材質としては、天然ゴムまたは各種の合成ゴ
ム例えばプチルデム等が使用される。As the material for the rubber stopper, natural rubber or various synthetic rubbers such as butyldem are used.
また前記被膜２をガラス管１内面とゴム栓体３との密着
面すなわち嵌合部にまで形成しても該栓体３のガラス管
１への液密な嵌着を可能とし、かつ該栓体３表面に血餅
等が付着しない様にする為に該栓体３のガラス管１側端
面および該ガラス管−１との密着面には水溶性シリコー
ンの被膜４が均一に形成されている。Furthermore, even if the coating 2 is formed on the contact surface between the inner surface of the glass tube 1 and the rubber stopper 3, that is, on the fitting part, it is possible to fit the stopper 3 into the glass tube 1 in a liquid-tight manner, and the stopper In order to prevent blood clots from adhering to the surface of the body 3, a water-soluble silicone coating 4 is uniformly formed on the end surface of the stopper 3 on the glass tube 1 side and on the surface in close contact with the glass tube 1. .
該水溶性シリコーン被膜４は前述の様な噴霧又は浸漬等
の方法により形成することができる。The water-soluble silicone coating 4 can be formed by the above-mentioned methods such as spraying or dipping.
試験例
採血管内面にｐｖｐの被膜を形成した本発明に係る採血
管とシリコーン被膜を施した採血管の採血遠心分離後の
血餅及びフィブリンの付着度合い並びに血液凝固所要時
間についての比較結果を表１に示す。Test Example Comparison results are shown regarding the degree of adhesion of blood clots and fibrin and the time required for blood coagulation after blood collection centrifugation between blood collection tubes according to the present invention with a PVP coating formed on the inner surface of the blood collection tube and a blood collection tube with a silicone coating. Shown in 1.
シリコーンの被膜を形成した採血管については血餅及び
フィブリンの付着が見られたが、ＰｖＰの被膜を形成し
たものについては付着はほとんど見うレず、特Ｋ ＰＶ
Ｐの被膜が１． ＯＸ １０−’ Ｉｎ９／ＣＩｒ１２
以上で形成されているものについては全く見られなかっ
た。またＰｖＰの被膜を形成した採血管はシリコーンの
被膜を形成した採血管に比し、凝固時間が短かいという
結果が得られた。なお、シリコーンはその被膜量を多く
することによシ血餅やフィブリンの付着を減少させるこ
とが可能であるが逆に凝固時間が長くなる為、実用に適
さなくなる０さらに他の試験において、ゴム栓体に水溶
性シリコーン被膜を形成した本発明に係る採血管は被膜
を形成しないものに比し、遠心分離後にゴム栓体への血
餅、フィブリン等の付着が見られなかった。なお、本試
験においてゴム栓体に使用した水溶性シリコーンの溶液
は濃度が約１チのものをｉ用した。Adhesion of blood clots and fibrin was observed on the blood collection tubes coated with silicone, but there was almost no adhesion observed on the tubes coated with PvP.
The P coating is 1. OX 10-' In9/CIr12
Nothing formed above was observed. Furthermore, the results showed that the blood collection tubes coated with PvP had a shorter coagulation time than those tubes coated with silicone. Although it is possible to reduce the adhesion of blood clots and fibrin by increasing the amount of silicone coating, it conversely prolongs the coagulation time, making it unsuitable for practical use.Furthermore, in other tests, silicone Compared to blood collection tubes according to the present invention in which a water-soluble silicone coating was formed on the stopper, no blood clots, fibrin, etc. were observed to adhere to the rubber stopper after centrifugation, compared to those without a coating. In addition, in this test, the water-soluble silicone solution used for the rubber stopper had a concentration of about 1 inch.
Ｗ９発明の具体的作用効果
以上のように、本発明の採血管は、水溶性高分子の被膜
２によシガラス管１の表面が覆われている為、ゴム栓体
３からの微量水分及び微量揮発物から隔離され、さらに
血清分離用の分離剤を管底に収容した場合においても該
分離剤からの揮発物から隔離されている為、ガラス管内
表面の清浄性が損われることがない。したがって、ガラ
ス管内の減圧状態を利用して所定量の血液を採血すれば
、水溶性高分子の被膜２が血液に溶解してあらかじめ洗
浄してあった清浄なガラス表面が露出し血液と接する。W9 Specific effects of the invention As described above, in the blood collection tube of the present invention, since the surface of the glass tube 1 is covered with the water-soluble polymer coating 2, trace amounts of moisture and traces from the rubber stopper 3 are removed. It is isolated from volatile substances, and even when a separating agent for serum separation is housed at the bottom of the tube, it is isolated from volatile substances from the separating agent, so the cleanliness of the inner surface of the glass tube is not impaired. Therefore, when a predetermined amount of blood is collected using the reduced pressure inside the glass tube, the water-soluble polymer coating 2 dissolves in the blood and the clean glass surface, which has been previously washed, is exposed and comes into contact with the blood.
清浄なガラス表面は、親水性であり、血液に対して非常
に°濡れ易く血液の凝固を促進すると共にフィブリン又
、は血餅の付着がない。他方、水溶性高分子波腹２に付
着していた揮発物は、当該被膜２の溶解と同時に血液中
に混入する。A clean glass surface is hydrophilic, highly wettable by blood, promotes blood coagulation, and is free of fibrin or blood clots. On the other hand, the volatile matter adhering to the water-soluble polymer antinode 2 mixes into the blood at the same time as the coating 2 dissolves.
また、ゴム栓体３のガラス管１側端面および該ガラス管
１内面との密着面には水溶性シリコーンの被膜４が形成
されている為、採血時に血液がガラス管１内に流入して
ゴム栓体と接触してもゴム栓体３の表面に血液が直接接
触することはない。In addition, since a water-soluble silicone coating 4 is formed on the end surface of the rubber stopper 3 on the side of the glass tube 1 and the surface in close contact with the inner surface of the glass tube 1, blood flows into the glass tube 1 during blood collection and the rubber Blood does not come into direct contact with the surface of the rubber stopper 3 even when it comes into contact with the stopper.
その為、ゴム栓体３の表面の微細な凹凸部分にはフィブ
リンや血餅等は付着せず、前記被膜４の表面に付着した
フィシリンや血餅等は遠心分離の際に剥離する。↓つて
遠心分離後のゴム栓体は清浄性が保たれフィブリンや血
餅等が付着していない為、ゴム栓体に水溶性シリコーン
の被膜を形成していない採血管の様に脱枠時に管壁との
摩擦によυフィブリンや血餅等が剥離して血清相中に混
入することもない。Therefore, fibrin, blood clots, etc. do not adhere to the fine irregularities on the surface of the rubber stopper 3, and ficillin, blood clots, etc. adhering to the surface of the coating 4 are peeled off during centrifugation. ↓After centrifugation, the rubber stopper remains clean and does not have fibrin or blood clots attached to it, so the rubber stopper is not coated with water-soluble silicone. Fibrin, blood clots, etc. do not come off due to friction with the wall and become mixed into the serum phase.
前記被膜４には前記水溶性シリコーンの他にグリセリン
を使用することも考えられるがグリセリンは検査値に影
響を与える場合があること、採血時に完全に溶解してし
まいゴム栓体３の表面が露出してしまうこと等を鑑みる
と水溶性シリコーンは遠心分離時にフィブリンや血餅等
が容易に剥離しない為、適さない。It is possible to use glycerin in addition to the water-soluble silicone for the coating 4, but glycerin may affect test values, and it completely dissolves during blood collection, exposing the surface of the rubber stopper 3. Water-soluble silicone is not suitable because fibrin, blood clots, etc. are not easily peeled off during centrifugation.
さらに被膜４はゴム栓体３からの揮発物等の放出を抑え
るという効果も有する。Furthermore, the coating 4 also has the effect of suppressing the release of volatile substances and the like from the rubber stopper 3.
また上記水溶性高分子が特にポリビニルピロリドンであ
る場合は水溶性に富み血漿増量剤としても使用されてい
るものであシ、血液に対して不活性であシ経時変化も少
ない。さらに採血管内に流入した血液と接触したときの
溶解性に優れ凝固を遅延させることがない。In addition, when the water-soluble polymer is particularly polyvinylpyrrolidone, it is highly water-soluble and is used as a plasma expander, is inactive with respect to blood, and has little change over time. Furthermore, it has excellent solubility when it comes into contact with blood flowing into the blood collection tube, and does not delay coagulation.
さらに上記ポリビニルピロリドンの被膜を採血管容器内
面に１．０Ｘ１０−−ν賞２以上で形成した場合はよシ
均一なばらつきのない被膜とすることができ、フィブリ
ンや血餅等の付着をより一層防止することができる。Furthermore, if the above-mentioned polyvinylpyrrolidone coating is formed on the inner surface of the blood collection tube container with a thickness of 1.0 x 10--ν 2 or more, it is possible to obtain a more uniform coating with no variation, which further prevents the adhesion of fibrin, blood clots, etc. It can be prevented.
以上述べた如く、本発明に係る採血管は採血管容器内面
に水溶性高分子の被膜が形成されている為、ゴム栓体か
ら生じる微量の水分及び微量揮発物から該容器内面が保
護され表面の清浄性が保た昨る。また、血清分離用の分
離剤を管底に収容した場合においても該分離剤から生ず
る揮発物から該容器内面を保護することができる。この
結果、採血時には水溶性高分子の被膜は血液に溶解して
清浄な容器内面が露出する為、遠心分離後に採血管内面
の血清帯域にフィブリンや血餅等が付着していることが
なく血清相を汚染することがない。As described above, since the blood collection tube according to the present invention has a water-soluble polymer coating formed on the inner surface of the blood collection tube container, the inner surface of the container is protected from trace amounts of moisture and trace amounts of volatile matter generated from the rubber stopper. Maintains cleanliness. Furthermore, even when a separating agent for serum separation is housed in the bottom of the tube, the inner surface of the container can be protected from volatile substances generated from the separating agent. As a result, when blood is collected, the water-soluble polymer coating dissolves in the blood and the clean inner surface of the container is exposed, so that after centrifugation, there is no fibrin or blood clots attached to the serum zone on the inner surface of the blood collection tube. Does not contaminate the phase.
なお、本発明に係る採血管は管内部を減圧した採血管に
限るものではなく他の採血管に適用しても同様な効果が
得られる。Note that the blood collection tube according to the present invention is not limited to a blood collection tube with a reduced pressure inside the tube, and similar effects can be obtained even when applied to other blood collection tubes.
また、採血管容器は遠心分離に適した管状容器であれば
良く材質は特に限定するものではないが、洗浄されたガ
ラス管の表面は親水性に優れ血液の凝固を遅延させるこ
とがない為、該容器をガラス管とするのが好ましい。In addition, the material of the blood collection tube container is not particularly limited as long as it is a tubular container suitable for centrifugation, but the surface of the cleaned glass tube is highly hydrophilic and does not delay blood coagulation. Preferably, the container is a glass tube.
また、前記水溶性高分子は採血管容器の内面全域に被膜
が形成され採血管内面のゴム栓体との密着面すなわち嵌
合部にも塗付することができる為、該密着面付近におけ
るフィシリンや血餅等の付着も防止することができる。In addition, the water-soluble polymer forms a coating over the entire inner surface of the blood collection tube container, and can also be applied to the surface of the blood collection tube that is in close contact with the rubber stopper, that is, the fitting portion, so that the ficilrin in the vicinity of the surface of the blood collection tube is in close contact with the rubber stopper. It can also prevent the adhesion of blood clots, etc.
さらに１、管状容器に嵌着されるゴム栓体は該栓体の該
容器側端面及び該容器内面との密着面に水溶性シリコー
ンの被膜が形成されている為、採血管内の全面に前記水
溶性高分子の被膜が形成されていても容易に着脱可能か
つ密封可能に嵌着することができる。Furthermore, 1. Since the rubber stopper fitted into the tubular container has a coating of water-soluble silicone formed on the end surface of the stopper on the side of the container and the surface in close contact with the inner surface of the container, the entire surface inside the blood collection tube is coated with water-soluble silicone. Even if a polymer film is formed, it can be easily attached and detached and hermetically fitted.
また、採血時に血液が採血管内に流入してゴム栓体と接
触した時ゴム栓体の表面は前記被膜により隔離されてい
る為、直接血液が接触することはない。よってゴム栓体
の表面の微細な凹凸部分にフィブリンや血餅等は付着せ
ず、前記被膜の表面に付着することになシ、付着したフ
ィブリンや血餅等は該被膜が水溶性シリコーンによ多形
成されている為、遠心分離時に剥離する、よって遠心分
離後のゴム栓体は清浄性が保たれフィブリンや血餅等が
付着していない。よって遠心分離後に脱枠する際にもフ
ィブリンや血餅等が血清相中に混入して検査値に影響を
与えることもない。Furthermore, when blood flows into the blood collection tube and comes into contact with the rubber stopper during blood collection, the surface of the rubber stopper is isolated by the coating, so the blood does not come into direct contact with it. Therefore, fibrin, blood clots, etc. do not adhere to the fine irregularities on the surface of the rubber stopper, and do not adhere to the surface of the coating. Since it is multiformed, it peels off during centrifugation, so the rubber stopper remains clean after centrifugation and is free of fibrin, blood clots, etc. Therefore, even when unframed after centrifugation, fibrin, blood clots, etc. do not get mixed into the serum phase and affect test values.
なおゴム栓体に形成された水溶性シリコーンの被膜は血
液流入時及び遠心分離時に溶解するが、ゴム栓体の表面
が露出することはなく水溶性シリコーンの薄膜が残る為
にゴム栓体表面は採血時及び遠心分離時を通じて常に保
護されている。Note that the water-soluble silicone coating formed on the rubber stopper dissolves during blood inflow and centrifugation, but the surface of the rubber stopper is not exposed and a thin film of water-soluble silicone remains. Protected at all times during blood collection and centrifugation.
さらに前記水溶性高分子がポリビニルピロリドンである
場合は水溶性に富み血液に対し不活性であシ経時変化も
少ない。さらに血液と接触したときの溶解性にも優れ、
血液凝固を遅延させない。Further, when the water-soluble polymer is polyvinylpyrrolidone, it is highly water-soluble, inert to blood, and changes little over time. Furthermore, it has excellent solubility when it comes into contact with blood,
Does not delay blood clotting.
また前記ポリピロリドンの被膜が１． Ｏｘ １０−５
ｍ９／ａｍ２以上で形成した場合はよシ均一なばらつき
のない被膜を形成することができる。Further, the polypyrrolidone coating 1. Ox 10-5
When formed with a ratio of m9/am2 or more, a more uniform coating without variation can be formed.
第１図は本発明の真空採血管を示す中央縦断面図である
。
図面中、１はガラス管、２は水溶性高分子の被膜、３は
ゴム栓、４はシリコーンの被膜である。FIG. 1 is a central vertical sectional view showing the vacuum blood collection tube of the present invention. In the drawings, 1 is a glass tube, 2 is a water-soluble polymer coating, 3 is a rubber stopper, and 4 is a silicone coating.
Claims (3)
可能に嵌着された弾性を有するゴム栓体とを有する採血
管において、 該栓体の該容器側端面および該容器内面との密着面に水
溶性シリコーンの被膜を形成するとともに、前記容器内
面全域には水溶性高分子の被膜を均一に形成したことを
特徴とする採血管。(1) In a blood collection tube having a tubular container and an elastic rubber stopper that is removably and sealably fitted into the opening of the container, the end surface of the stopper on the side of the container and the inner surface of the container A blood collection tube characterized in that a water-soluble silicone coating is formed on the contact surface, and a water-soluble polymer coating is uniformly formed over the entire inner surface of the container.
る特許請求の範囲第１項記載の採血管。(2) The blood collection tube according to claim 1, wherein the water-soluble polymer is &'J vinylpyrrolidone.
−３１ｖ／ｃＷＬ２以上で形成されている特許請求の範
囲第２項記載の採血管。(3) The above polyvinylpyrrolidone coating is 1.0×1O
The blood collection tube according to claim 2, which is formed of -31v/cWL2 or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56196307A JPS5897341A (en) | 1981-12-08 | 1981-12-08 | Blood sampling pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56196307A JPS5897341A (en) | 1981-12-08 | 1981-12-08 | Blood sampling pipe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5897341A true JPS5897341A (en) | 1983-06-09 |
| JPH0241331B2 JPH0241331B2 (en) | 1990-09-17 |
Family
ID=16355624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56196307A Granted JPS5897341A (en) | 1981-12-08 | 1981-12-08 | Blood sampling pipe |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5897341A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006161020A (en) * | 2004-11-15 | 2006-06-22 | Jsr Corp | Thermoplastic elastomer molded article, surface treatment method for thermoplastic elastomer molded article, medical tube, and infusion set |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0392313U (en) * | 1990-01-10 | 1991-09-19 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56104644A (en) * | 1980-01-28 | 1981-08-20 | Sekisui Chemical Co Ltd | Blooddcollecting tube for separating serum |
-
1981
- 1981-12-08 JP JP56196307A patent/JPS5897341A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56104644A (en) * | 1980-01-28 | 1981-08-20 | Sekisui Chemical Co Ltd | Blooddcollecting tube for separating serum |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006161020A (en) * | 2004-11-15 | 2006-06-22 | Jsr Corp | Thermoplastic elastomer molded article, surface treatment method for thermoplastic elastomer molded article, medical tube, and infusion set |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0241331B2 (en) | 1990-09-17 |
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