JPS5991817A - Cylindrical container for culturing seed strain - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a cylindrical container for culture of seed bacteria. The purpose of the present invention is to eliminate the disadvantages of the conventional seed culture container, which is robust and heat resistant and consists of a container body and a lid, and to improve the work of taking out the seed and growing the mushroom culture medium for the seed. The object of the present invention is to provide a cylindrical container for culture of seed bacteria that solves the difficulty of aseptic inoculation work.

The seed culture is generally cultivated by sterilizing a seed culture medium made of sawdust, rice bran, etc. filled in a container, then inoculating the seed mycelia into the seed medium, and then controlling it for a period of time. Conventionally, containers made of glass or plastic, which are durable and heat resistant, have been widely used as containers for seed culture. This is because the robust container retains its shape, making it easy to handle, and because the seed culture medium inside the container is stable against external shocks, unevenness in the medium and ventilation is unlikely to occur. However, when a container with such robustness is used as a seed culture container, there are the following drawbacks in the subsequent processing steps. That is, the seed fungus that has been formed by spreading the seed hyphae in the seed culture medium must be removed from the container, but since it is stuck to the inner wall of the container, the task of removing it is extremely difficult. In addition, the inoculum needs to be handled aseptically through the removal operation, the subsequent granulation operation, and the inoculation operation into the mushroom culture medium, but when using the conventional inoculum culture container, the Since removal work is essential, it is impossible to completely prevent bacterial contamination.

Furthermore, since containers are relatively expensive, they can be reused, but when using the conventional seed culture containers, there is a problem in that the containers must be cleaned and dried before reuse.

As a result of various studies to solve the above-mentioned drawbacks, the present inventor has developed a seed culture device that can achieve the intended purpose, in which a heat-resistant bag C can be fully inserted into the container body A, and This is a cylindrical container for seed culture, characterized in that the opening periphery 1 of the bag C is folded back to the outside of the opening 2 of the container body A.

The container body A and the lid B used in the present invention have robustness and heat resistance, and are made of, for example, glass or plastics such as polypropylene. Robustness is a requirement to give containers shape retention and impact resistance, as well as heat resistance.

The quality is a necessary requirement for sterilizing the seed culture medium in the container.

The bag C used in the present invention may be heat-resistant, such as plastic such as polypropylene or polyethylene (manufactured by Showa Denko Co., Ltd.), paper, or cloth.

Next, one embodiment of the present invention will be described based on the drawings. Figure 1 shows a front sectional view of a cylindrical container for seed culture with the simplest structure. Figure 2 is a front sectional view of a cylindrical container for seed culture with another structure. In figure 0 showing the plan view of the figure,
A indicates the container body, B indicates the lid, and C indicates the bag. Reference numeral 1 indicates the opening periphery of the bag C, which is folded back to the outside of the opening 2 of the container body A.

3 indicates the inoculation port, 4 indicates the cotton plug, and 5 indicates the seed culture medium. Reference numeral 6 indicates a vent provided on the top surface of the lid body B, 7 indicates a filter for purifying the air passing through the vent 6, and 8 indicates a cursor that can slide on an arm 9 provided on the lid B. .

Next, a method of using the cylindrical container for seed culture according to the present invention will be explained. Inserting the bag C into the container body A,
Fold the opening periphery 1 to the outside of the opening 2 of the container body A L, and fill the bag C with a starter culture medium 5 made of sawdust and rice bran L
After covering the container body A with the lid B (in the embodiments of FIGS. 2 and 3, the lid equipped with a filter), sterilization treatment is performed, and then seed mycelia are inoculated through the inoculation port 3. Then the first
In the embodiment shown in the figure, a cotton plug is attached to the inoculation port 3, and in the embodiment shown in Figs. 2 and 3, Kurtz/I/8
0 seed culture medium 5 to cover the inoculation port 3 and culture for a certain period of time.
After the seed mycelia have spread to C, the lid is removed and bag C is removed.
Gather the opening periphery 1 of the bag and tie it together [7. Alternatively, take out the bag C along with the contents from the container body A as it is, then crush the contents in the bag C, that is, the inoculum, and inoculate this into a mushroom culture made of logs. good.

Since the present invention has the above configuration, it has the following effects. That is, since the cylindrical container for seed culture according to the present invention consists of a robust container body and a static body, it has all the toilets of this type of conventional product. 1. Moreover, in the cylindrical container for seed culture according to the present invention, the bag is inserted into the container body, and the area around the opening of the bag is folded back to the outside of the opening of the container body, making it extremely easy to remove the seed bacteria. It is easy to carry out, and the work of inoculating the mushroom culture with this inoculum can be carried out aseptically.

Furthermore, in the present invention, there is no need to clean the container body, and since the bag is relatively inexpensive, it can be made disposable if necessary.

[Brief explanation of drawings]

FIG. 1 shows a front sectional view of a cylindrical container for seed culture according to the present invention, FIG. 2 is a front sectional view of a cylindrical container for seed culture according to another embodiment of the invention, and FIG. The top view of Figure 2 is shown. In the diagram, A...container body, B...lid, C...
... Bag 1... Around the opening, 2... Opening. Patent applicant Sachi Mushroom Garden Co., Ltd.

Claims (1)

[Claims] In a cylindrical container for seed culture having robustness and heat resistance and consisting of a container body A and a lid B, a heat resistant bag C is inserted into the container body A, and the opening periphery 1 of the bag C is inserted. A cylindrical container for seed culture, characterized in that the container main body A is folded back to the outside of the opening.