JPS60201257A - 特定のポリヌクレオチド配列順序の検出方法 - Google Patents

特定のポリヌクレオチド配列順序の検出方法

Info

Publication number: JPS60201257A
Authority: JP; Japan
Prior art keywords: sequence; dna; antibody; probe; rna
Prior art date: 1983-12-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP26009984A

Other languages

English (en)

Japanese (ja)

Inventor

ロバート・ジエイ・キヤリコ

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bayer Corp

Original Assignee

Miles Laboratories Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-12-12

Filing date

1984-12-11

Publication date

1985-10-11

1984-12-11 Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc

1985-10-11 Publication of JPS60201257A publication Critical patent/JPS60201257A/ja

Status Pending legal-status Critical Current

Links

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Landscapes

Investigating Or Analysing Biological Materials (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

JP26009984A 1983-12-12 1984-12-11 特定のポリヌクレオチド配列順序の検出方法 Pending JPS60201257A (ja)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date
US56042983A	1983-12-12	1983-12-12
US560429		1983-12-12
US668256		1984-11-07

Publications (1)

Publication Number	Publication Date
JPS60201257A true JPS60201257A (ja)	1985-10-11

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ID=24237794

Family Applications (4)

Application Number	Title	Priority Date	Filing Date
JP26009984A Pending JPS60201257A (ja)	1983-12-12	1984-12-11	特定のポリヌクレオチド配列順序の検出方法
JP26009784A Granted JPS60151559A (ja)	1983-12-12	1984-12-11	挿入複合体に対する抗体を使用する核酸ハイブリダイゼーシヨン分析
JP26009884A Pending JPS60201256A (ja)	1983-12-12	1984-12-11	特定のポリヌクレオチド配列の検出方法、検出用試薬系及び試験キツト
JP26010084A Pending JPS60179657A (ja)	1983-12-12	1984-12-11	標識プロ−ブ及び抗−ハイブリツドを用いるハイブリツド形成分析法

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Application Number	Title	Priority Date	Filing Date
JP26009784A Granted JPS60151559A (ja)	1983-12-12	1984-12-11	挿入複合体に対する抗体を使用する核酸ハイブリダイゼーシヨン分析
JP26009884A Pending JPS60201256A (ja)	1983-12-12	1984-12-11	特定のポリヌクレオチド配列の検出方法、検出用試薬系及び試験キツト
JP26010084A Pending JPS60179657A (ja)	1983-12-12	1984-12-11	標識プロ−ブ及び抗−ハイブリツドを用いるハイブリツド形成分析法

Country Status (2)

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JP (4)	JPS60201257A (da)
ZA (4)	ZA849595B (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4820630A (en) *	1984-11-23	1989-04-11	Digene Diagnostics, Incorporated	Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
FI72146C (fi) *	1985-01-02	1987-04-13	Orion Yhtymae Oy	Foerfarande foer identifiering av nukleinsyror.
JPS6446650A (en) *	1987-08-18	1989-02-21	Tosoh Corp	Nucleic acid hybridization assay method
JPH03130098A (ja) *	1989-06-30	1991-06-03	Sanyo Chem Ind Ltd	核酸の検出法及びび検出試薬
CN101467045A (zh) *	2006-06-15	2009-06-24	皇家飞利浦电子股份有限公司	通过测定结合和未结合的标记增加分析物检测的专一性

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4233402A (en) *	1978-04-05	1980-11-11	Syva Company	Reagents and method employing channeling
AU531777B2 (en) *	1978-04-05	1983-09-08	Syva Co.	Label/solid conjugate immunoassay system
US4299916A (en) *	1979-12-26	1981-11-10	Syva Company	Preferential signal production on a surface in immunoassays

1984
- 1984-12-10 ZA ZA849595A patent/ZA849595B/xx unknown
- 1984-12-10 ZA ZA849593A patent/ZA849593B/xx unknown
- 1984-12-10 ZA ZA849596A patent/ZA849596B/xx unknown
- 1984-12-10 ZA ZA849594A patent/ZA849594B/xx unknown
- 1984-12-11 JP JP26009984A patent/JPS60201257A/ja active Pending
- 1984-12-11 JP JP26009784A patent/JPS60151559A/ja active Granted
- 1984-12-11 JP JP26009884A patent/JPS60201256A/ja active Pending
- 1984-12-11 JP JP26010084A patent/JPS60179657A/ja active Pending

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIOCHEM.BIOPHYS.RES.COMMUN=1969 *
BIOCHEMISTRY=1978 *
BIOCHEMISTRY=1979 *
NUCLEIC ACID RESEACH=1982 *
PROC.NATL.ACAD.SCI.USA=1982 *

Also Published As

Publication number	Publication date
ZA849593B (en)	1985-07-31
JPS60151559A (ja)	1985-08-09
ZA849595B (en)	1985-07-31
JPS60201256A (ja)	1985-10-11
ZA849594B (en)	1985-08-28
ZA849596B (en)	1985-07-31
JPS60179657A (ja)	1985-09-13
JPH0531108B2 (da)	1993-05-11

Publication	Publication Date	Title
AU599013B2 (en)	1990-07-12	Solution-phase dual hybridization assay for detecting polynucleotide sequences
JP2801051B2 (ja)	1998-09-21	核酸塩基配列を検出するための方法及び試薬
US4743535A (en)	1988-05-10	Hybridization assay employing labeled probe and anti-hybrid
EP0163220B1 (en)	1990-06-20	Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
EP0144914A2 (en)	1985-06-19	Hybridization assay employing labeled pairs of hybrid binding reagents
US5985548A (en)	1999-11-16	Amplification of assay reporters by nucleic acid replication
EP1563100B1 (en)	2013-04-24	Displacement sandwich immuno-pcr
JP2577881B2 (ja)	1997-02-05	ポリヌクレオチド連鎖を利用する検定方法
US5200313A (en)	1993-04-06	Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4563417A (en)	1986-01-07	Nucleic acid hybridization assay employing antibodies to intercalation complexes
EP0146039A2 (en)	1985-06-26	Hybridization assay with immobilization of hybrids by antihybrid binding
JP2012120534A (ja)	2012-06-28	同種の分析物検出
JPS60262055A (ja)	1985-12-25	核酸のハイブリツド形成分析法およびそれに用いる試薬系
JPS62240864A (ja)	1987-10-21	エネルギ−伝達による分析物検出
JPS60144662A (ja)	1985-07-31	核酸プローブ、ポリヌクレオチド配列およびその抗体を検出するための試験方法および試薬系
WO1993015229A2 (en)	1993-08-05	Amplification of assay reporters by nucleic acid replication
EP0144913A2 (en)	1985-06-19	Hybridization assay employing labeled probe and anti-hybrid
EP0146815A2 (en)	1985-07-03	Nucleic acid hybridization assay employing antibodies to intercalation complexes
EP0237833A2 (en)	1987-09-23	Solution-phase hybridization assay for detecting polynucleotide sequences
JPS60201257A (ja)	1985-10-11	特定のポリヌクレオチド配列順序の検出方法
EP0209702A1 (en)	1987-01-28	Solid-phase hybridization assay using anti-hybrid antibodies
CA1250232A (en)	1989-02-21	Hybridization assay with immobilization of hybrids by anti-hybrid binding
CA1239580A (en)	1988-07-26	Hybridization assay employing labeled pairs of hybrid binding reagents
HK1003719B (en)	2000-06-09	Amplification of assay reporters by nucleic acid replication