JPS6032602B2 - Treatment for livestock skin mycosis - Google Patents

Description

[Detailed description of the invention] The present invention relates to a therapeutic agent for skin mycosis in livestock.

More specifically, 2-secondary butylphenyl-N-
The present invention relates to a therapeutic agent for external skin mycosis of domestic animals, which contains methyl carbamate (hereinafter abbreviated as BPMC) as an active ingredient. Dermatomycosis of livestock is a skin disease caused by infection with dermatophytes, and it causes lesions on the keratinized tissue of the skin.The lesions spread concentrically and become circular, hence the name ring hawk. , observed in livestock such as pigs, sheep, and goats.

Livestock infected with skin mycosis have poor eating habits due to symptoms such as a dull feeling in the affected area, a feeling of illness, irritation due to burning sensation, insomnia, etc., and malnutrition, resulting in decreased growth, fattening, and milk production. We will see a decline in In addition, there is a risk of secondary infection by other harmful bacteria, which also causes a significant decrease in the commercial value of livestock. In recent years, rationalization of land use has promoted intensive grazing, which has increased contact between individual livestock and further increased the risk of infection between livestock.

Furthermore, since these fungi are highly infectious to the human body, these fungal infections of livestock are also a major problem for those involved in the livestock industry.

The pathogen of dermatomycosis is a skin fungus (filamentous fungus), and the fungal body consists of hyphae and spores. The hyphae are found exclusively within the epidermis and epidermis, and the spores attach to the hair coat and also enter the hair shaft. There are many types of fungi and different classifications, but they can be broadly classified into the following types: a. micro-sporon
b. mchophyPn and c. yellow hawk fungus (
achorion) and usually takes the form of single or mixed infections of these bacterial species.

Treatment methods for skin mycosis in livestock include physical therapy such as ultraviolet rays and etc.), but it has no effect.

In recent years, preparations containing dimethyl 2,2,2-trichlor-1-oxyethylphosphonate (generic name trichlorfon), which is a type of organic compound, have been used as a treatment for skin mycosis in livestock. However, there are still unresolved problems in terms of efficacy, drug stability, toxicity, etc.

As mentioned above, the present inventors have searched for compounds that are more effective in treating skin mycosis in livestock, which causes great economic damage to livestock farmers and is also a major blow to the livestock industry. The use of BPMC achieves the intended purpose,
It was discovered that it has an unexpected effect on skin mycosis in livestock.

Therefore, an object of the present invention is to provide a new and effective therapeutic agent for skin mycosis in livestock. The compound BPMC (chemical name: 2-sec-butyl phenyl-N-methyl carbamate) used in the present invention has the following chemical formula (1
It is a type of carbamate drug represented by ), has a melting point of 3〆○, has a slight aroma, is soluble in acetone, toluene, xylene, etc., and is almost insoluble in water.

Regarding toxicity, BPMC has extremely low toxicity and is highly safe.

That is, the acute toxicity (LD rule oral) for male mice is 34
It has an o/k9 of 0, an o/k9 of 410 in male rats, and a dermal toxicity (LD5o) of 4,200 in mice. The outline and results of the antibacterial test (in vitro) of BPMC against livestock skin fungi are as follows.

Note that trichlorfon was set as a comparative compound. Anti-skin fungal test [1] Test compound BPMC Trichlorfon [2] Test skin fungus Trichoph onVermCOS YamamTrich
ophreonmbetemmMicrospommgyPS
eum Microsporumlangeronll [3]
Test method Official Methods of A
nal$is of AOAC (1973 King) F Saragi c
It was carried out according to the method of idaITesK51.

■ Purely culture the test skin fungus strain on potato dextrose agar slant medium.

After culturing at 25qo for 10 to 20 days (growth rate varies depending on the strain), store at 2 to 5°C.

■ Immediately before use, remove the bacterial strain from the sleeve, make a spore saline solution, and store it in a cool place (2-500℃).

■ Make 1M sonic dilutions of each test compound using distilled water to a maximum concentration of 1%. (Final concentration 1%, 0.1%, 0.
Prepare dilution solutions with four concentrations of 0.01% and 0.001%) ■ Dilution solution 1 of each concentration of the test compound prepared in ■
Add 0.1% of the spore saline solution of each strain prepared in ① to the top of the sensitization, stirring carefully. 15 minutes after the start, 6
After 0 minutes, 2 hours, and 4 hours, remove 1ase from each solution, wash it once or twice with saline, inoculate it on a brain heart infusion agar plate, and culture it at 2500℃ for 15 days. judge.

[4] Results The test results are shown in the table below.

The table shows the sensitization time of 15 minutes, 6 minutes, 2 days and 4 days for each skin fungus strain tested, and the concentration of the test compound is shown vertically.

In determining effectiveness -: Antibacterial effect Soil: Antibacterial effect is observed (bacterial growth is significant or is dying out.

) 10: No antibacterial effect shall be.

{l) Trichophyton VetruC0S
um(ii1 Trichophyton rubr
um(iiD MiCrOSp.

As is clear from the results of this test, BPMC has excellent antibacterial properties against skin fungi of livestock, and its action is stronger than that of trichlorfon. Next, the therapeutic effect of BPMC will be demonstrated by showing an example in which BPMC was used to treat a cow infected with a skin mycosis.

Note that trichlorfon was set as a comparative compound. Treatment of Bovine Skin Mycosis [1] Test Compound BPMC Trichlorfone BPMC was prepared as a liquid paraffin solution with a concentration of 1% and 0.1%.

After dissolving trichlorfon in a small amount of ethanol, liquid paraffin was added to give a concentration of 1% and o. It was made into a 1% solution.

[2] Target Animals Eight male Holstein cows, approximately 3 months old, with relatively severe symptoms, and considered to be in the early stages of pharyngeal disease were selected, and 2 were used for each concentration of the test drug.

[3] The solution of each concentration of the test compound prepared in treatment method [1] was applied to the affected area three times at one-week intervals, and the condition of the affected area was observed one week after the final application.

[4] Results The criteria for evaluation were the shedding of the skin and the state of hair growth on the affected area where the solution was applied.

The judgment criteria are as follows.

- No shedding of skin or hair growth was observed.

(10) Shedding of the skin was observed, but no hair growth was observed.

However, shedding of the skin and hair growth were observed.

Kawa: Shedding of the skin and significant hair growth were observed.

The results are shown in the table below. (iv) Microspo
rum langeroll From this result, BPMC
It has been shown to have excellent therapeutic effects, and its efficacy is superior to that of trichlorfon.

Examples of dosage forms for carrying out the present invention include external preparations such as powders, emulsions, liniments, wipes, ointments, pastes, liniments, lotions, wettable powders, and oils.

In this case, ordinary pharmaceutical techniques can be used for formulation. Next, examples of formulations containing BPMC as an active ingredient will be shown as examples.

Example 1 Emulsion Prescription: An emulsion is prepared by a conventional method.

Example 2 Prescription: A powder is prepared by a conventional method.

Example 3 Wettable powder formulation: Weight % of BPMC 4
White carbon 40 Alkylaryltriethine glycol 6 Sodium dodecylbenzenesulfonate 5 ″Polyvinyl alcohol
2. Prepare a hydrating powder using a conventional method.

Example 4 Oil agent Prescription: Prepare an oil solution using a conventional method.

Claims (1)

[Scope of Claims] 1. A therapeutic agent for skin mycosis of domestic animals, which contains 2-secondary-butylphenyl-N-methylcarbamate as an active ingredient. 2. The therapeutic agent for skin mycosis in domestic animals according to claim 1, which is an external preparation.