JPS6084227A - Stable aqueous extract of herb plant and its preparation - Google Patents

Abstract

PURPOSE:The titled extract containing an extract obtained by extracting a herb plant or a substance having a useful ingredient of it with an aqueous solution containing acetic acid as an active ingredient. CONSTITUTION:A stable aqueous extract of herb plant showing healthy effect, medicinal effect, nourishing effect, tonic effect, etc., similar to plants of Chinese orthodox medicine, of westernized Chinese traditional medicine, of folk medicine, of traditional medicine passed from generation to generation, etc., having solved troubles of occurrence of dregs, obtained by extracting a herb plant (e.g., ginseng, Ophiopogonis Tuber, Linderae Radix, Licorice, Rehmanniae Radix, Ledebouriellae Radix, etc.) or a useful ingredient of it with an aqueous solution containing acetic acid (e.g., 0.02-1W/V% acetic acid concentration), collecting the liquid phase. Acetic acid which is inexpensive, easily obtainable, and free from trouble with respect to safety attains stabilizing effect, and the stabilizing effect can be favorably kept even if the aqueous extract contains a component for drink or drinking agent. The acetic acid concentration is low, the aqueous extract has no bad influence at all on taste adaptability of drinking agent, etc. and it has merits to improve taste.

Description

[Detailed Description of the Invention] This research is based on, for example, Chinese medicinal plants, Nishijin Chinese medicinal plants,
Health effects, easy burial effects, such as folk medicines, traditional medicines, etc.
A maxim of useful ingredients that exhibits nourishing and tonic effects: fc'lk <
The present invention relates to an inexpensive aqueous herb plant extract that overcomes the troubles occurring in the first month, and a method for producing such an extract.

More particularly, the present invention relates to a stable aqueous harp plant extract which is characterized in that it contains as an active ingredient an extract of the harp plant or its useful constituents in an acetic acid-containing aqueous solution.

The present invention also relates to a method for producing the extract, and in particular, extracts a herb plant or its useful ingredient-containing substance with an acetic acid-containing aqueous solution with a low acetic acid concentration, collects the extract, and obtains an extract. The present invention also relates to a method for producing a stable aqueous herp plant extract, which comprises sterilizing the extract, or the extract obtained by adding ingredients for beverages or drink preparations to the extract.

Traditionally, herbal plants, such as the mulberry plant, have been used as ingredients for Western medicine since ancient times. In order to avoid the hassle of having to extract the herbal plants with an aqueous medium each time they are used, the herbal plants are extracted with an aqueous medium in advance.
If you try to preserve and use the aqueous extract containing useful components of the herb plant obtained by removing and separating the solid content, sludge will form over time, resulting in inconveniences such as deterioration of appearance and loss of palatability, making it difficult to consume. It is well known that there are problems that seriously impair the value of a product, such as making customers feel concerned about quality. Such troubles occur when the aqueous extract is treated with a clarifying agent, flocculant, etc. Even in extracts that have been further subjected to treatments such as chemical treatment and heat treatment to further remove the precipitated solid content, it is still difficult to avoid this problem, and this has become a bottleneck in the use of aqueous plant extracts. .

Herp plant aqueous extract contains a complex balance of a wide variety of natural ingredients, and as mentioned above, even if it is made into a clear extract, the balance of the complex natural ingredients will change over time. The appearance is earthy, and the balance of the clear aqueous extract cannot be maintained stably for a long time.

In this way, the actual state of potential number-forming components that may still cause image formation even in the aqueous extract of No. The details of the image formation mechanism in multi-component systems containing habitual precipitate-forming components are not known at all;
In such a complex natural source multi-component system, interactions and reactions between homogeneous components and/or different components, as well as colloidal chemical interactions, are intricately involved. To elucidate it is to make it unnecessary. Therefore, the guidelines for stabilizing the aqueous goo exudate of Nobu plants have not been known to date, and although numerous attempts have been made based on experience, no effective results have been found. Unfortunately, there is no specific countermeasure.

As a test, / Kotoe contains ingredients that have antioxidant effects such as vitamin L and vitamin C. : Attempts to add, for example, blocking contact with oxidizing gases such as air by filling with a non-oxidizing inert gas such as nitrogen gas, and/or blocking light rays by housing in a light-opaque or transparent spacer. Attempts to add chelating agents, such as ethylenediaminetetraacetate, and emulsion stabilizers, such as surfactants, have included, for example, heating/
Attempts have been made empirically to remove components that may precipitate as much as possible by repeating temperature change treatments such as cooling and freezing/thawing. Extraction *f* It was not possible to maintain the extract stably for a long period of time without the trouble of lees formation.

Historically, many of the latent precipitate-forming components that are responsible for image formation that still occur in aqueous extracts of herb plants, once the solids and solidifying components have been removed, have been found in the extracts from herbal plants. Normally, it is a useful ingredient, and if the above-mentioned densification treatment and its removal treatment are repeated,
Naturally, this results in a substantial loss of the useful components of the herbal plant, and the purpose of utilizing the aqueous herbal extract itself is lost.

The present inventors have proposed a solution to the above-mentioned problem of fatigue caused by Herp Plant 71 (sexual extract) without substantially destroying the useful components of the herbal plant contained in the extract. M research has been carried out to develop a technology that can provide a complete aqueous extract of harp plants that can overcome the safety concerns.

As a result, the Herb Plant 7 extract obtained by extracting the ingredients contained in Herb 411v/J or above with an acetic acid-containing aqueous solution, especially a low acetic acid-concentrated acetic acid-containing aqueous solution, has excellent stability. It was discovered that the aqueous extract of the harp plant exhibits the same properties and overcomes the problem of sludge formation.

Furthermore, although the mechanism of action by which this outstanding stability is achieved is completely unknown, we will discuss later in conjunction with the Examples that it is possible to take it into the body or that it can be taken in the body. sE (e.g. taste, smell, etc.)
We will give comparative examples using other 4'&Ub'r h that can be used in terms of this, and as shown experimentally, only vinegar is unique because it is cheap, easy to obtain, and has no problems in terms of safety. The achievement of the excellent redundant effect of the herbal plant aqueous extracts shown in Figure 1 was a completely unexpected and surprising result.

Furthermore, later on, when comparing the Examples and Comparative Examples, it was revealed that this effeminizing effect was caused by Yumei Moto's harp plant aqueous extract, or the distribution of beverages and drinks. The unexpected and surprising result was that the area could be maintained in good condition even when the area was laid apart. Therefore, according to the present invention, a stable herbal plant useful ingredient-containing beverage free from the trouble of lees formation, which has been desired since then but could not be provided. It turns out that drinks can be provided.

Furthermore, in addition to the fact that the acetic acid concentration of the acetic acid-containing aqueous solution used is low, the acetic acid concentration is low, so the resulting aqueous extract is suitable for use in beverages and drinks. It was found that there were no negative effects, and in fact, there was a benefit of improved singing and annotation.

(Thus, the purpose of the invention is to provide a method for injecting herbs with excellent stability.

Another object of the present invention is to provide a method for producing the herbal citrus water extract, which is used to produce the above-mentioned extract.

Further objects and many other objects and advantages of the invention will become more apparent from the description below.

The object to be extracted in this study is a herb plant or a substance containing useful components thereof. Nope plants, such as Chinese medicinal plants and Western Chinese herbal plants, are used for health, hygiene, pharmacology,
It has been used since ancient times as it contains useful ingredients that have energizing and tonic effects.

The stable aqueous harp plant extract of the present invention can be obtained by extracting the harp plant or its useful ingredient-containing components with an acetic acid-containing aqueous solution and collecting the liquid phase.

Preferably, a low acetic acid concentration, such as from about 0.02 to about 1
It can be obtained by extracting the herp plant or its useful ingredient content with an acetic acid-containing aqueous solution with a low acetic acid concentration, such as % w/v, and collecting the liquid phase. According to a preferred embodiment of the present study, the herp plant or its useful ingredient-containing substance is extracted with an acetic acid-containing aqueous solution containing a low concentration of tonic acid, and the +711 extract is collected.
l) The stable aqueous extract of the herb plant of the present invention can be produced by sterilizing the extract or the extract obtained by cooling the extract.

The half', 11α product or its useful ingredient-containing product can be used in any form), the plant body or its part containing the useful h' component of the 4i & product, its dried product, shredded product, crushed product, Juice, such as water, acids, alkalis, salts, sugars,
Examples include aqueous solutions such as alcohol and acetone, extract liquids obtained by adding alcohol, concentrates thereof, and spindle powders thereof. The parts for “-1・1” of the No-Zo plant are:
You can choose as appropriate depending on the type of plant, but? 8 For example,
Fruits, miko, their shells, stems, leaves, abyss, roots, flowers, flower buds,
Examples include flowers, rhizomes, roots, germs, bark, wood, and mushrooms.

U) Harp 1 As a direct example of fJ, the following harp plants can be shown. Carrot (commonly known as Kadzu root, Toki, Yocalyx, Yamanashi, Masuma, Hange, origin, raw strawberry)
) funnel root, sawara, gokapi, peony bark, thick paper, cinnamon bark, yellow oak, mulberry leaf, loquat leaf, persimmon leaf, sweet tea, young barley leaf, safflower, stock flower, Buddha vine, ephedra , Thin cargo, Innyangrei, Kai Ling, Boar, Reishi mushroom, Raigan, Shiitake mushroom, Kawaratake mushroom, Hiratake mushroom, Enoki mushroom, Uikiyo, Kijitsu, cinnamon bark, Chenpi, apricot fruit, peach fruit. , batomugi, aloe, T Yokobe, Genno Shoko, Senburi, Senna, Senega, Dokudami, Henkeku, Lobelia, Basu, Calapal beans, Set tonsils, Homica, Vanilla, Tamarind, Nomitsure, Saffron, Gentian, Kon Free, pine leaves, mugwort, garlic, chili peppers, chives, springs, horsetail, wheat germ, bean sprouts, etc.

Examples of acetic acid to be used for extraction include synthetic vinegar, fermented acetic acid, brewed vinegar, and sweet and sour vinegar. The amount to be used can be appropriately selected depending on the type, part, shape, etc. of the plant, but it is preferable to use it at a relatively low concentration. Herbal Plants [Kutsuto 1'l'l: Use at low levels of intoxicating acid so that the pH of the mixture of acid-containing aqueous M solution does not vary significantly from the pH of the daffodil extract of the herbal plants. is preferred, for example from about 0.02 to about 1 w/
A preferred example is an acetic acid concentration of v percent.

The immediate temperature of the harp plant can be selected as appropriate, and a bright room can be selected depending on the preparation, location, shape, etc. of the harp plant, but for one terry, the temperature should be around the boiling point at normal pressure, that is, around 100℃, and for cold immersion, the temperature should be at room temperature, e.g. Extraction processing temperatures such as 10° to about 30°C may be exemplified.

After the extraction process, collect the extract, strain it if desired,
Arrow P, sieving, cooling, centrifugation, natural filtration, pressure filtration,
Post-treatments such as pressure filtration and the like can be carried out to obtain a stable aqueous extract of the harp plant. The resulting extract can be heated with or without the addition of excipients such as lactose, starch, dextrose, milk powder, skim milk powder, CMC, casein, monoglycerides, sugar esters, etc. ``1'Q;;; It can be dried by means such as drying, freeze drying, and reduced pressure drying, or it can be dried under normal pressure.
Condensation can also be caused by means such as condensation, condensation, freeze concentration, etc.4-7.

According to one aspect of the invention, the above-mentioned method allows for the production of rotary acid in plants or plants containing useful ingredients (by imitation of sex baths). (take liquid) cunningly;
; + <I extract, or add 1.1X, a minute to 10% Qj to make it stable. ) 1-P plant aqueous extract can be prepared 91.1.

Examples of the necessary ingredients for such drinks or all drinks include the following ingredients. Sake, alcoholic beverages such as 1 pe of grapes, sugars such as citrus sugar, succulent sugar, sucrose, invert sugar, grape frame, fructose, friend sugar, sorbitol, honey, etc. Amino acids such as sodium glutamate, lysine, and aspartic acid, organic acids such as citric acid, tartaric acid, and lactic acid, thickening agents such as alginic acid, CMC, guar gum, gelatin, agar, and pectin, thiamine hydrochloride, riboflavin, vitamin C, and vitamin E, etc. Vitamins, preservatives such as benzoic acid and paraoxybenzoic acid, other fragrances, and pigments.The above-mentioned sterilization treatment includes, for example, low-wash 1 sterilization and high-temperature sterilization in accordance with the denticidal conditions based on the Food Sanitation Act. Examples of treatment methods include high-temperature short-time sterilization and high-temperature instant sterilization.

The stable herp plant aqueous extract of the present invention has a wide range of uses as general beverages, nutritional tonic drinks, health drinks, herbal medicine drinks, Chinese herbal drinks, medicinal drinks, sports drinks, etc., and as a component thereof. .

Hereinafter, several embodiments of the present invention will be explained in more detail using examples as well as comparative examples.

Example 1, Control Examples, and Comparative Examples 1 to 3 Korean ginseng (chopped), Goji leaves (shredded), Sonoka and Hatomugi (skinned)
Mix and distribute 4 herbal medicines, each with 10F each.
Five sets of Of' are prepared and divided into 1 to 5 sections. 1 category was tap water (control example), 2 categories were 0.2% acetic acid aqueous solution (Example 1), and 3 categories were 0.2% tartaric acid aqueous solution (Comparative example 1)
, 4 categories include 0.2% citric acid aqueous solution (Comparative Example 2), 5
600 tnl of 0.2% malic acid water bath solution (Comparative Example 3) (tap water was used) was added to each section, and heated for 90 minutes while continuing to gently boil with a backflow cooler. After this was left overnight, it was suction-filtered repeatedly on P paper covered with silica clay until the P solution became completely clear and transparent to obtain a crude drug extract health and tonic drink stock solution. The properties of the obtained drink concentrate are shown in Table 1.

Divide each stock solution into three equal parts, heat sterilize one at 85°C for 40 minutes and seal it to make a drink stock solution, and add 5 $ of glucose per liquid volume to the other for flavoring and nutritional enrichment. w/v, sugar s% w/τ, quail 7 acid 0
．． 2% w/τ, ascorbic acid 0.05 $ w/
v, thiamine nitrate o, oo1%W/υ, riboflavin 0
．． 001%, filtered, heat sterilized at 85°C for 4'0 minutes, and sealed tightly to create a health tonic containing herbal medicine extract.

Use as a linking agent. Both were stored in the refrigerator and the state of precipitation of the lees was observed. The results are shown in Table 2.

As is clear from Table 2, various organic acid-containing liquids are added to the extraction water to which the aqueous extracts of the combined herbal medicines are extracted, to the extent that the pH of the extraction liquid is not substantially changed. As a result of examining the effectiveness of preventing sludge precipitation during long-term storage of herbal medicine extract products, it was found that only products extracted with acetic acid-added aqueous extraction solution at the end of the machine could produce products that did not show sludge or cloudiness even after long-term storage. It turned out that it was possible. The resulting product had no acetic acid odor at all.

Example 2, Control Examples, and Comparative Examples 4 to 6 Five groups of 100 pieces of mushrooms belonging to the Sarunokoshikake mushroom "Kawaratake" were collected,
Divide into 1 to 5 categories. Water was passed through water in the first category (control example), and brewed rice vinegar was used in the second category with an acetic acid concentration of 0. Vinegar-containing aqueous solution (Example 2) added to IW/υ ratio, 0 in the third category
．． 1 w/v aqueous tartaric acid solution (comparative example 4), 0.1 w/v % citric acid solution in the fourth section (comparative example 5, o, 1 w/v % gluconic acid solution (comparative example 5),
Comparative Example 6) (all using tap water), 1500 tons of each
nl was added, and a backflow condenser was attached for about 90 minutes to perform heating extraction to the extent that boiling did not stop. Each extract was filtered through four layers of gauze F:, and the F solution was repeatedly suctioned onto a paper covered with sand and gravel until the lachrymal fluid was completely clarified and became transparent.
Five sets of N fjl transparent Kawaratake extract liquids were obtained. Each set of extract liquid f: was divided into two equal parts, and one half was heat sterilized at 85°C for 40 minutes and sealed to produce a bamboo shoot extract product. On the other hand, glucose 6 $w/v, lug honey 4% W/V, citric acid o, i% W/υ, and ascorbic acid 0.05% w/v based on the liquid volume for flavoring and nutritional enrichment. ,
Thiamin acid 0.001%w/v, Su? Frapin o
, ooi%W/υ, and a flavoring agent were added, heat sterilized at 85° C. for 40 minutes, and sealed. These were stored in a refrigerator and a 37° C. thermostat, and the precipitation state of # (oli) was observed over time. The properties of the extract are shown in Table 3, and the precipitation status of the drink is shown in Table 4.
Shown in the table.

As is clear from Table 4, in both the undiluted solution and the drink, sedimentation of lees was observed within 3 months even when stored at 38°C under refrigeration except for the rice vinegar extract, but no sedimentation was observed in the rice vinegar-added extract. There wasn't.

LOL Example 3 and Comparative Example 7 Korean #lOgu, Hakusui 101, Purchase 7.52, Return 7.
5t, Chen BL5y, Daiju 5g, Saiko 52, Licorice Z5F,
Weigh and mix a total of 557 of the above-mentioned raw iron, with a total of φ1.22 of reincarnation and 1.32 square meters of raw iron, and mix raw mulberry A and B2 for the Chinese herbal medicine Sohochuekkito.
Prepare the tissue. Tap water for group A is approximately 800 rn, e (
Hiko father'fIJ'r), td O,l W/v to IB
% Vinegar 1 liter water solution approx. 8001ne (Fu〃1 old; Wasu3)
Add 7III to the thighs and boil gently for about 90 minutes.
heat. In case of fever, use four layers of gauze to cover the area. Pg, was repeatedly filtered on a tear paper covered with diatomaceous earth, and the P solution was completely clarified and filtered through reeds, and the amount of ascorbic acid, o s w/v %, and glucose 6% based on the liquid volume was lol
Add 4% w/v of honey and an appropriate amount of fragrance to dissolve, filter, and dispense about 60ml of the slurry into small bottles, tighten the stopper lightly, heat sterilize at 85°C for 30 minutes, and then seal the bottle. Each group.

In total, 10 bottles of Hochuekkito and two Chinese herbal medicine drinks were prepared. Five of the 10 bottles in each set were stored in a refrigerated container, and 5 were stored in a 37° C. incubator, and the contents were observed over time.

The progress is shown in Table 5.

As shown in Table 5, Group B, LC4.1w/v% West''
``Hochuekkito liquid extracted with an acid aqueous solution and a drink using it did not form any lees even after three months.

Procedural amendment September 12, 1981 Patent Office is? 'U Utsugi Otsuo L, Indication of the case, Patent Application No. 143835/1983, Relationship with the Nagisa case to be amended Patent applicant 4, Agent 〒107 (1) Specification No. 1, pages 6 to 7 On the line, after the word ``for example,'' add ``about 0.01 to about 2 cents w/v, more preferably J.

Claims (1)

[Scope of Claims] 1. A stable aqueous extract of a herb plant, characterized in that it contains as an active ingredient a product extracted from the acetic acid-containing water extract 7 of a herb plant or its useful components. 2. The acetic acid content of the acetic acid-containing aqueous bath solution is about 0.02 to about 1 w/v percent, Claim 1
Aqueous extract as described in Section. a The aqueous acetic acid according to claim 1 or 2, wherein the acetic acid is at least one type of vinegar selected from the group consisting of @/A# acid, fermented acetic acid, brewed vinegar, and synthetic vinegar. Extract. 4. The aqueous extract according to any one of claims 1, 2, and 3, wherein the aqueous extract is in the form of a drink or a drink containing the ingredients for the drink or drink. Extract. 5. Harp plants or their useful ingredient-containing substances,
Collecting the extract by soaking it in an acetic acid-containing aqueous bath solution with a low acetic acid concentration, or sterilizing the extract obtained by adding ingredients for beverages or drink preparations to the extract. A method for producing a stable aqueous extract of the Hague plant with sex characteristics.