JPS61190858U - - Google Patents
Info
- Publication number
- JPS61190858U JPS61190858U JP7537185U JP7537185U JPS61190858U JP S61190858 U JPS61190858 U JP S61190858U JP 7537185 U JP7537185 U JP 7537185U JP 7537185 U JP7537185 U JP 7537185U JP S61190858 U JPS61190858 U JP S61190858U
- Authority
- JP
- Japan
- Prior art keywords
- anolyte
- storage section
- insulating members
- dimensional
- dimensional electrophoresis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000539 two dimensional gel electrophoresis Methods 0.000 claims description 4
- 238000001125 extrusion Methods 0.000 claims description 3
- 125000006850 spacer group Chemical group 0.000 claims description 3
- 239000003566 sealing material Substances 0.000 claims description 2
- 238000001962 electrophoresis Methods 0.000 claims 1
- 239000011810 insulating material Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000005012 migration Effects 0.000 claims 1
- 238000013508 migration Methods 0.000 claims 1
Landscapes
- Joining Of Glass To Other Materials (AREA)
- Peptides Or Proteins (AREA)
Description
第1図はこの考案に係る二次元電気泳動装置の
一実施例を示す説明断面図、第2図a,bはこの
一方の絶縁部材とスペーサの正面図及び平面図、
第3図はこの他方の絶縁部材の正面図、第4図a
,bはこの考案に用いられる一次元目のゲルと二
次元目のゲルの正面図及び側面図である。
1……二次元電気泳動装置、2……スペーサ、
3,4……絶縁部材、5……押圧手段、6……陽
電極、7……陰電極、8……電圧印加手段、9…
…押出手段、10……シール材、11……陰極液
収納部、12……二次元目のゲル収納部、13…
…陽極液収納部、14……陽極液供給路、15…
…開閉弁。
FIG. 1 is an explanatory cross-sectional view showing one embodiment of a two-dimensional electrophoresis device according to this invention, FIGS. 2a and 2b are a front view and a plan view of one insulating member and a spacer,
Figure 3 is a front view of this other insulating member, Figure 4a
, b are a front view and a side view of a first-dimensional gel and a second-dimensional gel used in this invention. 1... Two-dimensional electrophoresis device, 2... Spacer,
3, 4... Insulating member, 5... Pressing means, 6... Positive electrode, 7... Negative electrode, 8... Voltage applying means, 9...
...Extrusion means, 10...Sealing material, 11...Catholyte storage section, 12...Second-dimensional gel storage section, 13...
...Anolyte storage section, 14...Anolyte supply path, 15...
...Open/close valve.
Claims (1)
れた絶縁材からなるスペーサと、このスペーサを
介して対向し上部が開放した箱状を形成しこの箱
内にその上部から下方に向かつて順に陰極液収納
部、二次元目のゲル収納部及び陽極液収納部を形
成する2つの平板状の絶縁部材と、この一方の絶
縁部材の陽極液収納部に対応する部位に開口し外
部に延出された開閉弁付の陽極液供給路と、前記
両絶縁部材を対向する方向に押圧する押圧手段と
、前記陽極液収納部内及び陰極液収納部内に設け
られた陽電極及び陰電極と、この両電極間に泳動
電圧を印加する電圧印加手段と、二次元目の電気
泳動終了後に陽極液収納部内を加圧して二次元目
のゲルを両絶縁部材の間から外部に押し出す押出
手段とを備えてなる二次元電気泳動装置。 2 押出手段が、陽極液供給路から陽極液収納部
内に気体又は液体を圧送する圧送手段からなる請
求の範囲第1項記載の二次元電気泳動装置。[Claims for Utility Model Registration] 1. A spacer made of an insulating material that is approximately U-shaped and has a sealing material attached to its outer periphery; From the top to the bottom, there are two flat insulating members forming, in order, a catholyte storage section, a second-dimensional gel storage section, and an anolyte storage section, and one of the insulating members corresponds to the anolyte storage section. an anolyte supply channel with an on-off valve that opens at a portion where the anolyte is to be disposed and extends to the outside; a pressing means that presses both the insulating members in opposing directions; An anode and a cathode, a voltage application means for applying a migration voltage between the two electrodes, and after the second-dimensional electrophoresis is completed, the inside of the anolyte storage part is pressurized to spread the second-dimensional gel from between the two insulating members. A two-dimensional electrophoresis device comprising extrusion means for extruding to the outside. 2. The two-dimensional electrophoresis device according to claim 1, wherein the extrusion means comprises a pressure-feeding means for force-feeding gas or liquid from the anolyte supply path into the anolyte storage section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7537185U JPH0353163Y2 (en) | 1985-05-20 | 1985-05-20 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7537185U JPH0353163Y2 (en) | 1985-05-20 | 1985-05-20 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61190858U true JPS61190858U (en) | 1986-11-27 |
| JPH0353163Y2 JPH0353163Y2 (en) | 1991-11-20 |
Family
ID=30616532
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7537185U Expired JPH0353163Y2 (en) | 1985-05-20 | 1985-05-20 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0353163Y2 (en) |
-
1985
- 1985-05-20 JP JP7537185U patent/JPH0353163Y2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0353163Y2 (en) | 1991-11-20 |