JPS6125497A - ス−パ−オキシドジスムタ−ゼ活性測定法 - Google Patents

ス−パ−オキシドジスムタ−ゼ活性測定法

Info

Publication number: JPS6125497A
Authority: JP; Japan
Prior art keywords: reagent; solution; serum; superoxide dismutase; added
Prior art date: 1984-07-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

JP14829384A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0569520B2 (de

Inventor

Toshiro Hanada

寿郎花田

Kazuhiko Yamanishi

山西　一彦

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Fujifilm Wako Pure Chemical Corp

Original Assignee

Wako Pure Chemical Industries Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-07-16

Filing date

1984-07-16

Publication date

1986-02-04

1984-07-16 Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd

1984-07-16 Priority to JP14829384A priority Critical patent/JPS6125497A/ja

1986-02-04 Publication of JPS6125497A publication Critical patent/JPS6125497A/ja

1993-10-01 Publication of JPH0569520B2 publication Critical patent/JPH0569520B2/ja

Status Granted legal-status Critical Current

Links

230000000694 effects Effects 0.000 title claims abstract description 26
102000019197 Superoxide Dismutase Human genes 0.000 title claims abstract 11
108010012715 Superoxide dismutase Proteins 0.000 title claims abstract 11
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims abstract description 24
LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 claims abstract description 20
229920000858 Cyclodextrin Polymers 0.000 claims abstract description 13
108010093894 Xanthine oxidase Proteins 0.000 claims abstract description 11
102100033220 Xanthine oxidase Human genes 0.000 claims abstract description 11
125000003831 tetrazolyl group Chemical group 0.000 claims abstract description 11
229940075420 xanthine Drugs 0.000 claims abstract description 10
OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 claims abstract description 6
238000000034 method Methods 0.000 claims description 50
238000006243 chemical reaction Methods 0.000 claims description 16
HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 8
VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 claims description 6
230000001603 reducing effect Effects 0.000 claims description 6
BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 claims description 6
150000002460 imidazoles Chemical class 0.000 claims description 5
-1 superoxide anions Chemical class 0.000 claims description 3
108010075031 Cytochromes c Proteins 0.000 claims 1
230000000087 stabilizing effect Effects 0.000 claims 1
238000006911 enzymatic reaction Methods 0.000 abstract description 38
210000002966 serum Anatomy 0.000 abstract description 37
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 abstract description 26
229960002885 histidine Drugs 0.000 abstract description 14
WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 abstract description 10
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239000003153 chemical reaction reagent Substances 0.000 description 83
239000000243 solution Substances 0.000 description 36
238000002835 absorbance Methods 0.000 description 29
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239000012089 stop solution Substances 0.000 description 20
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 18
230000005764 inhibitory process Effects 0.000 description 18
238000000691 measurement method Methods 0.000 description 15
230000000052 comparative effect Effects 0.000 description 13
239000007864 aqueous solution Substances 0.000 description 10
238000005259 measurement Methods 0.000 description 10
229960005070 ascorbic acid Drugs 0.000 description 9
235000010323 ascorbic acid Nutrition 0.000 description 9
239000011668 ascorbic acid Substances 0.000 description 9
150000003839 salts Chemical class 0.000 description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
239000008363 phosphate buffer Substances 0.000 description 7
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 7
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
150000001875 compounds Chemical class 0.000 description 6
108010010803 Gelatin Proteins 0.000 description 5
HNDVDQJCIGZPNO-UHFFFAOYSA-N Histidine Chemical compound OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 5
230000002378 acidificating effect Effects 0.000 description 5
229920000159 gelatin Polymers 0.000 description 5
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235000019322 gelatine Nutrition 0.000 description 5
235000011852 gelatine desserts Nutrition 0.000 description 5
239000000344 soap Substances 0.000 description 5
239000000126 substance Substances 0.000 description 5
PKDBCJSWQUOKDO-UHFFFAOYSA-M 2,3,5-triphenyltetrazolium chloride Chemical compound [Cl-].C1=CC=CC=C1C(N=[N+]1C=2C=CC=CC=2)=NN1C1=CC=CC=C1 PKDBCJSWQUOKDO-UHFFFAOYSA-M 0.000 description 4
239000002253 acid Substances 0.000 description 4
239000011734 sodium Substances 0.000 description 4
229910052708 sodium Inorganic materials 0.000 description 4
235000019333 sodium laurylsulphate Nutrition 0.000 description 4
238000010186 staining Methods 0.000 description 4
239000004094 surface-active agent Substances 0.000 description 4
108010052832 Cytochromes Proteins 0.000 description 3
102000018832 Cytochromes Human genes 0.000 description 3
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
239000013522 chelant Substances 0.000 description 3
239000003795 chemical substances by application Substances 0.000 description 3
238000007323 disproportionation reaction Methods 0.000 description 3
MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 3
239000007788 liquid Substances 0.000 description 3
239000002736 nonionic surfactant Substances 0.000 description 3
239000001301 oxygen Substances 0.000 description 3
229910052760 oxygen Inorganic materials 0.000 description 3
239000012085 test solution Substances 0.000 description 3
DEPDDPLQZYCHOH-UHFFFAOYSA-N 1h-imidazol-2-amine Chemical compound NC1=NC=CN1 DEPDDPLQZYCHOH-UHFFFAOYSA-N 0.000 description 2
102000004506 Blood Proteins Human genes 0.000 description 2
108010017384 Blood Proteins Proteins 0.000 description 2
BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
BRMWTNUJHUMWMS-LURJTMIESA-N N(tele)-methyl-L-histidine Chemical compound CN1C=NC(C[C@H](N)C(O)=O)=C1 BRMWTNUJHUMWMS-LURJTMIESA-N 0.000 description 2
239000013504 Triton X-100 Substances 0.000 description 2
229920004890 Triton X-100 Polymers 0.000 description 2
150000007513 acids Chemical class 0.000 description 2
LPTWEDZIPSKWDG-UHFFFAOYSA-N benzenesulfonic acid;dodecane Chemical compound OS(=O)(=O)C1=CC=CC=C1.CCCCCCCCCCCC LPTWEDZIPSKWDG-UHFFFAOYSA-N 0.000 description 2
210000004369 blood Anatomy 0.000 description 2
239000008280 blood Substances 0.000 description 2
239000000872 buffer Substances 0.000 description 2
238000001514 detection method Methods 0.000 description 2
229910001882 dioxygen Inorganic materials 0.000 description 2
GDSRMADSINPKSL-HSEONFRVSA-N gamma-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO GDSRMADSINPKSL-HSEONFRVSA-N 0.000 description 2
229940080345 gamma-cyclodextrin Drugs 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
229910021645 metal ion Inorganic materials 0.000 description 2
102000039446 nucleic acids Human genes 0.000 description 2
108020004707 nucleic acids Proteins 0.000 description 2
150000007523 nucleic acids Chemical class 0.000 description 2
238000012360 testing method Methods 0.000 description 2
KKKDZZRICRFGSD-UHFFFAOYSA-N 1-benzylimidazole Chemical compound C1=CN=CN1CC1=CC=CC=C1 KKKDZZRICRFGSD-UHFFFAOYSA-N 0.000 description 1
MCTWTZJPVLRJOU-UHFFFAOYSA-N 1-methyl-1H-imidazole Chemical compound CN1C=CN=C1 MCTWTZJPVLRJOU-UHFFFAOYSA-N 0.000 description 1
SEULWJSKCVACTH-UHFFFAOYSA-N 1-phenylimidazole Chemical compound C1=NC=CN1C1=CC=CC=C1 SEULWJSKCVACTH-UHFFFAOYSA-N 0.000 description 1
NMIZONYLXCOHEF-UHFFFAOYSA-N 1h-imidazole-2-carboxamide Chemical class NC(=O)C1=NC=CN1 NMIZONYLXCOHEF-UHFFFAOYSA-N 0.000 description 1
KYWMCFOWDYFYLV-UHFFFAOYSA-N 1h-imidazole-2-carboxylic acid Chemical compound OC(=O)C1=NC=CN1 KYWMCFOWDYFYLV-UHFFFAOYSA-N 0.000 description 1
AXHRGVJWDJDYPO-UHFFFAOYSA-N 2-bromo-1h-imidazole Chemical compound BrC1=NC=CN1 AXHRGVJWDJDYPO-UHFFFAOYSA-N 0.000 description 1
LXBGSDVWAMZHDD-UHFFFAOYSA-N 2-methyl-1h-imidazole Chemical compound CC1=NC=CN1 LXBGSDVWAMZHDD-UHFFFAOYSA-N 0.000 description 1
AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 1
JJMQRJKPLUACSO-UHFFFAOYSA-N 3-(4-iodophenyl)-2-(4-nitrophenyl)-5-phenyl-1,3-dihydrotetrazol-3-ium;chloride Chemical compound [Cl-].C1=CC([N+](=O)[O-])=CC=C1N1N(C=2C=CC(I)=CC=2)[NH2+]C(C=2C=CC=CC=2)=N1 JJMQRJKPLUACSO-UHFFFAOYSA-N 0.000 description 1
NYDGOZPYEABERA-UHFFFAOYSA-N 5-chloro-1-methylimidazole Chemical compound CN1C=NC=C1Cl NYDGOZPYEABERA-UHFFFAOYSA-N 0.000 description 1
VYDWQPKRHOGLPA-UHFFFAOYSA-N 5-nitroimidazole Chemical compound [O-][N+](=O)C1=CN=CN1 VYDWQPKRHOGLPA-UHFFFAOYSA-N 0.000 description 1
XHLKOHSAWQPOFO-UHFFFAOYSA-N 5-phenyl-1h-imidazole Chemical compound N1C=NC=C1C1=CC=CC=C1 XHLKOHSAWQPOFO-UHFFFAOYSA-N 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
YIOMLQGQTLNUFX-UHFFFAOYSA-N CCCCCCCC(CC)OS(O)(=O)=O Chemical compound CCCCCCCC(CC)OS(O)(=O)=O YIOMLQGQTLNUFX-UHFFFAOYSA-N 0.000 description 1
208000005623 Carcinogenesis Diseases 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical class [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
108010022613 Cytochrome d Group Proteins 0.000 description 1
HNDVDQJCIGZPNO-RXMQYKEDSA-N D-histidine Chemical compound OC(=O)[C@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-RXMQYKEDSA-N 0.000 description 1
229930195721 D-histidine Natural products 0.000 description 1
241000196324 Embryophyta Species 0.000 description 1
BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 description 1
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ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
102000010750 Metalloproteins Human genes 0.000 description 1
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UEZVMMHDMIWARA-UHFFFAOYSA-N Metaphosphoric acid Chemical compound OP(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-N 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
JDHILDINMRGULE-LURJTMIESA-N N(pros)-methyl-L-histidine Chemical compound CN1C=NC=C1C[C@H](N)C(O)=O JDHILDINMRGULE-LURJTMIESA-N 0.000 description 1
229920001744 Polyaldehyde Polymers 0.000 description 1
PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
230000002730 additional effect Effects 0.000 description 1
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BTBJBAZGXNKLQC-UHFFFAOYSA-N ammonium lauryl sulfate Chemical compound [NH4+].CCCCCCCCCCCCOS([O-])(=O)=O BTBJBAZGXNKLQC-UHFFFAOYSA-N 0.000 description 1
229940063953 ammonium lauryl sulfate Drugs 0.000 description 1
150000001450 anions Chemical class 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
PLUHAVSIMCXBEX-UHFFFAOYSA-N azane;dodecyl benzenesulfonate Chemical compound N.CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 PLUHAVSIMCXBEX-UHFFFAOYSA-N 0.000 description 1
210000001124 body fluid Anatomy 0.000 description 1
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230000007797 corrosion Effects 0.000 description 1
238000005260 corrosion Methods 0.000 description 1
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125000002704 decyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
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PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 1
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FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 description 1
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230000002829 reductive effect Effects 0.000 description 1
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239000012266 salt solution Substances 0.000 description 1
229940083542 sodium Drugs 0.000 description 1
238000005063 solubilization Methods 0.000 description 1
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238000010561 standard procedure Methods 0.000 description 1
238000003860 storage Methods 0.000 description 1
BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
230000008719 thickening Effects 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
LOIYMIARKYCTBW-OWOJBTEDSA-N trans-urocanic acid Chemical compound OC(=O)\C=C\C1=CNC=N1 LOIYMIARKYCTBW-OWOJBTEDSA-N 0.000 description 1
LOIYMIARKYCTBW-UHFFFAOYSA-N trans-urocanic acid Natural products OC(=O)C=CC1=CNC=N1 LOIYMIARKYCTBW-UHFFFAOYSA-N 0.000 description 1
229940116269 uric acid Drugs 0.000 description 1
210000002700 urine Anatomy 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

JP14829384A 1984-07-16 1984-07-16 ス−パ−オキシドジスムタ−ゼ活性測定法 Granted JPS6125497A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP14829384A JPS6125497A (ja)	1984-07-16	1984-07-16	ス−パ−オキシドジスムタ−ゼ活性測定法

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP14829384A JPS6125497A (ja)	1984-07-16	1984-07-16	ス−パ−オキシドジスムタ−ゼ活性測定法

Publications (2)

Publication Number	Publication Date
JPS6125497A true JPS6125497A (ja)	1986-02-04
JPH0569520B2 JPH0569520B2 (de)	1993-10-01

Family

ID=15449532

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP14829384A Granted JPS6125497A (ja)	1984-07-16	1984-07-16	ス−パ−オキシドジスムタ−ゼ活性測定法

Country Status (1)

Country	Link
JP (1)	JPS6125497A (de)

1984
- 1984-07-16 JP JP14829384A patent/JPS6125497A/ja active Granted

Also Published As

Publication number	Publication date
JPH0569520B2 (de)	1993-10-01

Legal Events

Date	Code	Title	Description
2004-07-16	EXPY	Cancellation because of completion of term

Publication	Publication Date	Title
Drummond et al.	1995	Determination of phosphorus in aqueous solution via formation of the phosphoantimonylmolybdenum blue complex. Re-examination of optimum conditions for the analysis of phosphate
Matsubara et al.	1985	Spectrophotometric determination of hydrogen peroxide with titanium 2-((5-bromopyridyl) azo)-5-(N-propyl-N-sulfopropylamino) phenol reagent and its application to the determination of serum glucose using glucose oxidase
JPS6134799B2 (de)	1986-08-09
JPS60184400A (ja)	1985-09-19	新規な発色試薬
US4622296A (en)	1986-11-11	Process for measuring activity of dehydrogenase employing a reaction stopper
Duijndam et al.	1975	The interaction of apurinic acid aldehyde groups with pararosaniline in the Feulgen-Schiff and related staining procedures
White-Stevens et al.	1982	Interference by ascorbic acid in test systems involving peroxidase. II. Redox-coupled indicator systems.
Ohlsson et al.	1983	The reduction potential of lactoperoxidase
US5151370A (en)	1992-09-29	Reagent and method for serum iron assay
JPS6125497A (ja)	1986-02-04	ス−パ−オキシドジスムタ−ゼ活性測定法
KR100379646B1 (ko)	2003-07-12	시약 조성물, 시험편 및 측정 키트
EP0989189B1 (de)	2006-06-07	Reagentienzusammensetzung zum bestimmen von elektrolyten
Gebicki et al.	1996	The limitations of an iodometric aerobic assay for peroxides
CN119061112B (zh)	2025-10-03	一种α-L-岩藻糖苷酶检测试剂或试剂盒及其制备方法
JPS62190099A (ja)	1987-08-20	ス−パ−オキシドジスムタ−ゼ活性測定法
JP3541677B2 (ja)	2004-07-14	直接型ビリルビンの測定方法および測定用試薬
Buchanan et al.	1976	The effects of pH and temperature on the assay of superoxide dismutase
JPS62210988A (ja)	1987-09-17	キサンチンオキシダ−ゼの安定化方法
JPS5822200B2 (ja)	1983-05-07	血清成分の測定方法
JPS6188153A (ja)	1986-05-06	生体微量成分の定量法
CA1204048A (en)	1986-05-06	Method for the detection and removal of substitution labile transition metal ions
CA2170110A1 (en)	1996-08-24	Decreased interference redox detection system
US4207203A (en)	1980-06-10	Uric acid standard solutions
Gaokar et al.	1986	Rapid spectrophotometric determination of cerium (III) with 4-(2-thiazolylazo) resorcinol and 1-(2-thiazolylazo)-2-naphthol
JPH0455192B2 (de)	1992-09-02