JPS6128865A - Method and apparatus for monitoring precipitant in purified plasma - Google Patents

Abstract

PURPOSE:To treat a large amount of plasma continuously in safety by a method wherein a part of purified plasma is extracted continuously to react with a precipitant detection liquid and changes in the transparency thereof is monitored to take a safety measure immediately if any change is found. CONSTITUTION:A part of treated plasma cleared of a precipitant with a plasma component adjustor 8 is extracted in branch with a sampling pump 10 and converged with a precipitant detection solution to react with a reactor 13. Based on the drop in the transparency of the plasma caused by any precipitant left therein, changes in the transparency thereof are detected with a detector comprising a light emitting section 14 and a light receiving section 14' to be outputted to a control section 15. When the precipitate is left so much as to impair a human body, an alarm 16 is actuated to take a safety measure immediately. This enables the monitoring of purified plasma with a relatively handy device thereby treating a large amount of plasma continuously and in safety.

Description

Detailed Description of the Invention 10 Background of the Invention (1) Technical Field The present invention relates to a method and device for monitoring precipitants in purified plasma, and in particular to a method for selectively precipitating specific components in plasma using sulfate. The present invention relates to a precipitant monitoring method and apparatus for continuously monitoring whether a desalination system is functioning normally after removal.

The precipitant monitoring method and device of the present invention are used to remove pathogenic substances from a large amount of self-collected plasma and to ensure safety during re-injection.

Further, the precipitant monitoring method and apparatus of the present invention are used to remove pathogenic substances from a large amount of raw plasma to ensure safety when purifying the plasma.

(2) Prior art and its problems Specific components in plasma, especially immunoglobulins, immune complexes,
Various immune and metabolic B-related disorders are thought to be caused by 21Y, which accumulates complement and other soluble polymer substances.
Plasmapheresis therapy has been attempted in recent years to treat patients' plasma, but fresh frozen plasma as a replacement fluid is expensive and in short supply, and side effects are frequent. Many studies have been conducted on plasma purification methods that selectively remove substances (especially high-molecular protein components) that are considered to be harmful to human blood and convert autologous plasma. Currently, membrane separation methods and adsorption separation methods are being widely studied as plasma purification methods, but the former has a large separation amount but poor selectivity, and the latter has excellent selectivity but a small separation amount, making both of them practical. It's not complete.

On the other hand, it is known that the above substances are precipitated by metal salts, organic solvents, acids, etc., and these precipitants are added to patient plasma, the ionic strength and pH are adjusted, and the precipitate formed is separated, followed by dehydration. One possible method would be to remove the precipitant and return it to the Tang party. Organic solvents and acids as precipitants have the risk of causing protein denaturation and require careful handling and are somewhat complicated, but metal salts can be said to be an extremely safe and useful method if desalted sufficiently. However, there is a danger if the desalination process works incompletely, and unless continuous monitoring is possible, the only way to confirm safety is to use a batch method, which makes it difficult to return plasma through continuous processing. there were.

■1 Purpose of the Invention When specific components (particularly albumin and other plasma proteins) are to be removed by precipitation from plasma, sulfates, especially ammonium sulfate, sodium sulfate, etc., are easily used as good and stable substances.

The purpose of the present invention is to monitor the residual sulfate ions in the continuous pipe after the desalination process using a simple method when such a precipitant is used, and to issue an alarm if the sulfur salt concentration exceeds a specified amount. Another object of the present invention is to provide a precipitant monitoring device in purified plasma that can take safety measures such as stopping the purifying device and the device.

In order to achieve the above object, the present invention consists of the following configuration.

(1) After selectively precipitating and removing specific components in plasma using a precipitant C agent, the amount of precipitant in purified plasma that has been further treated with a precipitant is continuously monitored. In the method, a step of continuously extracting a part of the purified plasma, and feeding the extracted purified plasma at a predetermined flow rate and a precipitant detection solution at a constant ratio to the predetermined flow rate. a step of introducing the purified plasma and the precipitant detection solution into a mixer and reacting the precipitant in the purified plasma with the detection agent in the precipitant detection solution; 1. A method for monitoring a precipitant in purified plasma, comprising a step of constantly monitoring changes in transparency and performing a predetermined treatment for safety when it is detected that the transparency is below a predetermined value.

(2) A precipitant monitor in purified plasma that continuously monitors the amount of precipitant in purified plasma that has been further treated with a descipitant after selectively removing specific components in plasma using a precipitant. The apparatus includes a plasma pump that continuously extracts and delivers a portion of the purified plasma, and a sedimentation pump that detects a precipitant in the purified plasma.

C agent detection solution, and a detection solution pump that is linked to the plasma pump and delivers the precipitant detection solution at a constant delivery ratio;
A mixer that mixes the purified plasma and the precipitant detection solution that has been sent and reacts the detection agent in the precipitant detection solution with the precipitant in the purified plasma, and a mixer that reacts with the precipitant in the purified plasma, and a change in the transparency of the purified plasma due to the reaction. a detection unit that constantly monitors and outputs a detection signal when it detects that the transparency is below a predetermined value; and a detection unit that controls the drive of the plasma pump and the detection solution pump and receives the detection signal of the detection unit. A precipitant monitoring device in purified plasma, comprising a control unit that outputs a predetermined signal of a safety reservoir.

(3) The apparatus for monitoring precipitants in purified plasma according to item 2, wherein the precipitant solution is an inorganic sulfate solution and the precipitant detection solution is a saturated aqueous solution of barium salts excluding barium sulfate.

(4) The precipitant monitoring device in purified plasma according to item 2 or 3, wherein the control unit outputs a signal for stopping supply of purified plasma and an alarm signal.

(9) Detailed Description and Function of the Invention The present invention will be specifically explained below with reference to embodiments shown in the drawings.

The figure is a configuration diagram of a blood purification device including an embodiment of the present invention. In the figure, 1 is a blood pump that introduces dehydrated blood into, for example, a vinyl chloride acid wood tube 101 and sends the liquid; 2;
3 is a centrifugal or deformed plasma separator that separates the introduced blood into plasma and blood cell components; 3 is a centrifugal or deformed plasma separator for discharging the separated plasma into a vinyl chloride side pipe 102; a double-roller type plasma pump that sends and returns the same amount of processed plasma as the liquid plasma; 4 is a container storing a precipitant solution consisting of, for example, a sulfate-saturated aqueous solution; 5;
6 is a precipitant solution pump that is linked to the plasma pump 3 and feeds the precipitant solution in the container 4 at a constant liquid feeding ratio; 6 is a mixer that mixes the pumped plasma and the precipitant solution; 7 is a mixing device; Vessel 6
8 is a plasma component regulator that removes the precipitant from the filtered plasma and adjusts the water content and electrolytes; 9 is the processed plasma after adjustment; In the blood purification system equipped with the sixth embodiment, which is a plasma blood mixer that mixes blood into concentrated blood consisting of blood cell components, the precipitant is removed in the plasma component regulator 8, and the water content and electrolytes are adjusted. A small portion of the processed plasma is diverted to side v103 in order to further monitor the suitability of the desalination process.

In the figure, IO is a sampling pump that branches a part of the plasma treated with a descipitating agent to a side pipe 103 made of vinyl chloride, and 11 is a precipitating agent detection solution preferably made of a saturated aqueous solution of barium salt. The container housing the container 2 is a detection solution pump that is linked to the sampling pump 10 and feeds the precipitant detection solution in the container 11 at a constant liquid feeding ratio.

13 is a reactor that mixes the pumped plasma and precipitant detection solution and reacts the precipitant remaining in the processed plasma with the precipitant and detection agent; 14 is a reactor that uses light such as a laser, etc. 13 is the light emitting part that irradiates the reactor 13, and 14' is the reactor 13.
a light receiving section that receives scattered light or transmitted light through the
Reference numeral 15 indicates a control unit that constantly monitors the detection signal of the light receiving unit 14' and controls the drive of each of the above-mentioned infusion pumps 3.5, 10.12, etc., and 16 indicates a control unit that is used when the control unit 15 detects an abnormality in the desalination process. This is an alarm to notify the outside of an abnormality.

The above configuration ensures safe operation of the sitting liquid purification system provided with this embodiment. That is, the patient's liquid, which has been drained by the blood pump 1, is led to the plasma separator 2, where it is separated into blood cells and plasma components. The separated plasma is sent to plasma pump 3.
At the same time, the precipitant solution is delivered to the side pipe 102 by the precipitant solution pump 5, which rotates at a constant ratio in conjunction with the plasma pump 3, and mixed in the mixer 6. As a result, specific components (especially globulin fraction) in the plasma that have been selectively precipitated are filtered by a plasma filtration filter 7, and the filtered plasma is led to a plasma component regulator 8 where precipitant-dissolved water and precipitate are removed. The amount of water removed is automatically adjusted by the double-roller plasma pump 3, which sends and returns the same amount of processed plasma as the plasma sent to the side pipe 102.In this way, When the plasma component regulator 8 is functioning normally and the precipitant in the processed disintegration is below the specified concentration, the purified plasma flowing out from the plasma component regulator 8 is mixed with plasma and blood by the plasma pump 3. The blood flows into the vessel 9, joins with blood cell components concentrated in the plasma separator 2, and is returned to the human body.

However, if the precipitant in the treated plasma exceeds a specified concentration, safety measures such as immediately stopping the blood purification system are taken. That is, in order to monitor whether the desalting process described above is being carried out normally, a part of the treated plasma from which the precipitant has been removed by the plasma component regulator 8 is branched and extracted by the sampling pump IO, and sampling pump 1o
The reactor 13 is fed with the precipitant detection solution sent by the detection agent solution pump 12 which rotates at a constant feed rate in conjunction with the
be merged at At this time, if there is no precipitant (especially sulfate) remaining in the treated plasma at a concentration higher than the specified concentration, no significant decrease in transparency will be observed in the mixture of the plasma and the precipitant detection solution. The mixed liquid is discharged as is. However, if the precipitant remains in the processed plasma at a concentration higher than the specified concentration, the purified plasma will become cloudy due to insoluble substances generated by reaction with the detection agent (especially barium salt) in the reactor 13. Transparency is significantly reduced. This change in transparency is detected as a change in the amount of transmitted light by a detector consisting of a light emitting section 14 and a light receiving section 14', and a signal to that effect is output to a control section 15. The control unit 15 learns from this signal that the treated plasma contains a precipitant at a concentration higher than the specified concentration that is undesirable for the human body, activates the alarm 16, and activates the pumps 3, 5, 10, 12 and pump 1. Activate safety devices such as stopping the

■Specific Effects of the Invention As described above, according to the present invention, the concentration of precipitants, especially sulfur salts, in purified plasma can be continuously monitored, so a large amount of plasma can be processed continuously and safely. patient safety can be ensured.

Furthermore, according to the present invention, the concentration of the precipitant in purified plasma is detected based on the change in transparency caused by the reaction between the precipitant and the detection agent, so this can be achieved without the need for an expensive analyzer. can.

[Brief explanation of the drawing]

The figure is a configuration diagram of a blood purification device including an embodiment of the present invention. Explanation of symbols of main parts

Claims (4)

[Claims] (1) A method for monitoring precipitants in purified plasma, which involves selectively removing precipitants from specific components in plasma using a precipitant, and then continuously monitoring the amount of precipitants in purified plasma that has been further treated with a precipitant. , a step of continuously extracting a part of the purified plasma, and a step of feeding the extracted purified plasma at a predetermined flow rate and a step of feeding a precipitant detection solution at a constant ratio to the predetermined flow rate. a step of introducing the purified plasma and the precipitant detection solution into a mixer and reacting the precipitant in the purified plasma with the detection agent in the precipitant detection solution; 1. A method for monitoring a precipitant in purified plasma, comprising a step of constantly monitoring changes in transparency and performing a predetermined treatment for safety when it is detected that the transparency is below a predetermined value. (2) A precipitant monitoring device for purified plasma that selectively removes specific components in plasma using a precipitant and then continuously monitors the amount of precipitant in purified plasma that has been further treated with a precipitant. a plasma pump that continuously extracts and delivers a portion of the purified plasma; a precipitant detection solution that detects a precipitant in the purified plasma; a detection solution pump that delivers the precipitant detection solution at a ratio; and a detection solution pump that mixes the purified plasma and the precipitant detection solution that have been delivered, and mixes the precipitant in the purified plasma with the detection agent in the precipitant detection solution. a mixer for reacting, a detection unit that constantly monitors changes in the transparency of the purified plasma due to the reaction and outputs a detection signal when the transparency is detected to be below a predetermined value, the plasma pump and the detection solution pump. 1. A precipitant monitoring device for purified plasma, comprising a control section that controls the drive of the precipitant and outputs a predetermined signal for safety when receiving a detection signal from the detection section. (3) A precipitant monitoring device in purified plasma according to claim 2, wherein the precipitant solution is an inorganic sulfate solution and the precipitant detection solution is a saturated aqueous solution of barium salts excluding barium sulfate. . (4) The precipitant monitoring device in purified plasma according to claim 2 or 3, wherein the control section outputs a signal for stopping supply of purified plasma and an alarm signal.