JPS6140786A - Method for cultivating basidiomycete - Google Patents
Method for cultivating basidiomyceteInfo
- Publication number
- JPS6140786A JPS6140786A JP16169384A JP16169384A JPS6140786A JP S6140786 A JPS6140786 A JP S6140786A JP 16169384 A JP16169384 A JP 16169384A JP 16169384 A JP16169384 A JP 16169384A JP S6140786 A JPS6140786 A JP S6140786A
- Authority
- JP
- Japan
- Prior art keywords
- basidiomycetes
- culture medium
- medium
- soybean broth
- mycelia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000221198 Basidiomycota Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 11
- 244000068988 Glycine max Species 0.000 claims abstract description 16
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 abstract description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 150000003839 salts Chemical class 0.000 abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 2
- 229940041514 candida albicans extract Drugs 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 239000008103 glucose Substances 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 239000012138 yeast extract Substances 0.000 abstract description 2
- 241000654305 Daedaleopsis styracina Species 0.000 abstract 1
- 241000222635 Lenzites betulinus Species 0.000 abstract 1
- 239000001888 Peptone Substances 0.000 abstract 1
- 108010080698 Peptones Proteins 0.000 abstract 1
- 241000222355 Trametes versicolor Species 0.000 abstract 1
- 229910010272 inorganic material Inorganic materials 0.000 abstract 1
- 239000011147 inorganic material Substances 0.000 abstract 1
- 235000019319 peptone Nutrition 0.000 abstract 1
- 230000004526 pharmaceutical effect Effects 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 17
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 5
- 235000013402 health food Nutrition 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 240000008397 Ganoderma lucidum Species 0.000 description 3
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 3
- 238000012136 culture method Methods 0.000 description 3
- 239000003925 fat Substances 0.000 description 3
- 235000019197 fats Nutrition 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 235000002639 sodium chloride Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 240000000599 Lentinula edodes Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 241000121220 Tricholoma matsutake Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 238000007664 blowing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
- 235000000023 Auricularia auricula Nutrition 0.000 description 1
- 240000005710 Auricularia polytricha Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 240000006499 Flammulina velutipes Species 0.000 description 1
- 235000016640 Flammulina velutipes Nutrition 0.000 description 1
- 240000001080 Grifola frondosa Species 0.000 description 1
- 235000007710 Grifola frondosa Nutrition 0.000 description 1
- 235000001715 Lentinula edodes Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 229940099112 cornstarch Drugs 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229940075427 peptone,dried Drugs 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000012265 solid product Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は種々の医薬的効果を有する担子菌類の菌糸を効
率よく増殖させる担子菌類の培養分法に関するものであ
る。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for culturing basidiomycetes that efficiently propagates hyphae of basidiomycetes that have various medicinal effects.
椎茸、松茸などの担子菌類は古くから食品として用いら
れており、またサルノコシカケなどは漢方薬として服用
されていた。更に近時サルノコシカケなどの担子菌類の
成分が抗腫瘍性を有することが知られ、これらの担子菌
類を培養し、それから有効成分を抽出する方法が多く開
示されている。Basidiomycetes such as shiitake mushrooms and matsutake mushrooms have been used as food since ancient times, and basidiomycetes such as mushrooms have been taken as herbal medicine. Furthermore, it has recently been known that components of basidiomycetes such as Basidiomycetes have antitumor properties, and many methods have been disclosed for culturing these basidiomycetes and extracting active ingredients from them.
また、担子菌類のこのような食品、医薬的有効性に鑑み
、担子菌類を培地に接種増殖させ、その有効成分を抽出
して保健食品とする方法も開示されている。例えば特開
昭５４−１１２５０ 、５４−４６８５９゜５４−４６
８６０ 、５４−１３５２５７号公報。In addition, in view of the food and medicinal efficacy of basidiomycetes, a method has also been disclosed in which basidiomycetes are inoculated into a culture medium, grown, and their active ingredients are extracted to produce health foods. For example, JP-A-54-11250, 54-46859゜54-46
860, No. 54-135257.
上述の如く、担子菌類の培養物が医薬、保健食品の供給
源として重要な価値を有して来ているが、現在その培養
法としては、従来一般に用いられていた培地を用いてい
るに過ぎず、需要の増大して来た担子菌類の培養には更
に菌糸の増殖のよい効率の向上した培養法が要望されて
いる。As mentioned above, cultures of Basidiomycetes have been of great value as a source of medicines and health foods, but the current culture methods are limited to those that use conventionally commonly used media. First, for the cultivation of basidiomycetes, which are in increasing demand, there is a need for a culture method with improved efficiency that allows for better mycelial growth.
本発明は前記問題点を解決する目的で、担子菌類の増殖
を増進する培地につき研究したところ、培地中に大豆煮
汁を加えて、該培地に担子菌種を接種し培養した結果、
接種担子菌が顕著に増殖した。In order to solve the above-mentioned problems, the present invention conducted research on a medium that promotes the growth of basidiomycetes, and found that by adding soybean broth to the medium and inoculating and culturing the basidiomycete species into the medium,
The inoculated basidiomycetes grew significantly.
本発明は大豆煮汁を含む培地に担子菌を接種し、菌糸を
増殖させることにより担子菌を効率よく培養する方法で
ある。The present invention is a method for efficiently culturing basidiomycetes by inoculating them into a medium containing soybean broth and growing mycelia.
本発明に用いる担子菌として、マツタケ、シイタケ、エ
ノキタケ、ナメコクケ、マンネンタケ。Examples of basidiomycetes used in the present invention include Matsutake, Shiitake, Enokitake, Namekoke, and Cinnamon.
カワラクケ、マイタケ、サルノコシカケ、キクラゲ、−
シロキクラゲなどがあげられる。好適にはサルノコシカ
ケ、カワラタケなどである。Kawarakake, Maitake, Sarunokoshikake, Wood ear mushroom, −
Examples include white fungus. Suitable mushrooms include Sarunokoshikake and Kawaratake.
本発明における担子菌を接種、繁殖させる培地は一般の
微生物の培養に使用する培地が用いられる。例えば炭素
源としてグルコース、グリセリン。As the medium for inoculating and propagating the basidiomycete in the present invention, a medium used for culturing general microorganisms is used. For example, glucose and glycerin as carbon sources.
澱粉、デキストリン、オートミル、 ＄Ｊ！ｉ蜜、油脂
。Starch, dextrin, oatmil, $J! i Honey, fats and oils.
脂肪など、窒素源として、綿実粕、ペプトン、乾燥酵母
、コーンステイープリカー、酵母エキス。Nitrogen sources such as fats, cottonseed meal, peptone, dried yeast, cornstarch liquor, and yeast extract.
カゼインなどの有機物、硫酸アンモニウム、硝酸アンモ
ニウムなどの無機物が使用できる。また必要に応じて、
炭酸カルシウム、食塩、塩化カリウム、りん酸塩、硫酸
マグネシウム、その他微量金属塩などの無機塩類を添加
するほか、担子菌の生育を助けるアミノ酸、ビタミンな
どの有機物及び無機物を適宜添加する。本発明において
は、このような一般の組成の培地に、大豆煮汁５〜５０
％（重量）、好適には１０％加えたものである。Organic substances such as casein and inorganic substances such as ammonium sulfate and ammonium nitrate can be used. Also, if necessary,
In addition to adding inorganic salts such as calcium carbonate, common salt, potassium chloride, phosphate, magnesium sulfate, and other trace metal salts, organic and inorganic substances such as amino acids and vitamins that help the growth of basidiomycetes are added as appropriate. In the present invention, soybean broth of 5 to 50% is added to a medium having such a general composition.
% (by weight), preferably 10%.
本発明の培養法は、上述の培地に担子菌を接種し、約１
５〜３５℃、好ましくは約２０〜３０℃、ｐＨ５〜９、
好ましくば７前後で、５〜１５日間空気を吹込みながら
培養すると担子菌が増殖する。In the culture method of the present invention, basidiomycetes are inoculated into the above-mentioned medium, and approximately
5-35°C, preferably about 20-30°C, pH 5-9,
Preferably around 7, basidiomycetes will proliferate if cultured for 5 to 15 days while blowing air.
本発明方法により得られた担子菌菌糸が増殖した培地は
培養終了ｆ＆７０〜９０℃で５〜２０分加熱処理して、
そのままホモミキサーなどの破砕機により菌糸体を破砕
し、凍結乾燥又は減圧乾個して固形物とするか、破砕後
乾燥することなく、破砕菌糸の懸濁溶液として保健食品
とする。The medium in which the basidiomycete hyphae obtained by the method of the present invention have grown is heat-treated at 70 to 90°C for 5 to 20 minutes at the end of culture.
The mycelia are crushed as is using a crusher such as a homomixer, and the mycelium is freeze-dried or dried under reduced pressure to obtain a solid product, or the crushed mycelium is used as a suspension solution of the crushed mycelium without drying, which is used as a health food.
また、前記加熱処理した担子菌培養物を連続遠心分離し
、分離液をそのまま、又は分離液を凍結乾燥或いは減圧
乾個し保健食品などとする。このようにして得られた保
健食品は担子菌の有する医薬、栄養的効果と併せて、培
地に添加された大豆煮汁中の大豆サポニンを含有し極め
て有用である。Further, the heat-treated basidiomycete culture is subjected to continuous centrifugation, and the separated liquid is used as it is, or the separated liquid is freeze-dried or dried under reduced pressure to be used as a health food. The health food thus obtained is extremely useful as it contains the medicinal and nutritional effects of the basidiomycete as well as the soybean saponin in the soybean broth added to the culture medium.
例１
ブＶ’ｙｔｊＭ３０ｇ、 ヘア” ） ７２ ｇ、 酵
ＲＩ−！−ス２ ｇ、 ＫＨ２ＰＯ４１ｇ、Ｍ
ｇＳＯ４・７Ｈ２０Ｑ、５ｇ、 ＣａＣＯ３０，１ｇ、
ＣａＣｌ２ ０．１ｇを蒸溜水で全量を１ ｋｇとし
、これに大豆煮汁（蛋白ｆｉ：１９．５１％、脂質９．
４５％、炭水化物１４．９７％、水分５３％）２５ｇを
加えた培地を１２０℃、２０分間加熱滅菌し、これに予
め純粋培養した霊芝を接種し、除菌した空気を吹込みな
がら、２８’Ｃで８日間ｐＨ７で培養した。Example 1 Bu V'ytjM 30g, hair'') 72g, fermented RI-!-su 2g, KH2PO41g, M
gSO4・7H20Q, 5g, CaCO30, 1g,
Add 0.1 g of CaCl2 to 1 kg with distilled water, and add soybean broth (protein fi: 19.51%, fat 9.5%) to this.
45%, carbohydrates 14.97%, moisture 53%) was added to the medium, heat sterilized at 120°C for 20 minutes, inoculated with pre-purely cultured Ganoderma lucidum, and while blowing sterilized air, the medium was heated for 28 hours. 'C at pH 7 for 8 days.
本発明方法によると担子菌の糸菌の増殖が極めて促進さ
れる。According to the method of the present invention, the growth of basidiomycete filamentous fungi is extremely promoted.
次に実施、例１の培地組成において、大豆煮汁１０％添
加した培地２５ ｋｇを用い、霊芝を接種し、温度２８
°Ｃで２０１！、／分通気しながら８日間培養し、仕込
み時、ｌ ２．３，４．５．６，７．８日目の時間毎
の多糖及び菌糸体の量を測定した。Next, using the culture medium composition of Example 1, 25 kg of the culture medium to which 10% soybean broth was added was inoculated with Ganoderma lucidum, and the temperature was 28.
201 at °C! The cells were cultured for 8 days while aerating the cells for 2.3, 4.5.6, and 7.8 days.
なお、対象として、大豆汁を添加しない他は上記と同一
培地、同一条件で霊芝を同様に培養し、仕込み時、１，
２，３，４，５，６，７．８日の時間毎の多糖及び菌糸
体の量を測定した。その結果を第１図で示す。As a subject, Reishi mushroom was cultivated in the same manner as above in the same medium and under the same conditions except that soybean juice was not added, and at the time of preparation, 1,
The amounts of polysaccharide and mycelium were measured every 2, 3, 4, 5, 6, and 7.8 days. The results are shown in FIG.
この試験で明らかな通り、担子菌の培地に大豆煮汁を添
加して培養する本発明の方法において、ＩＩ子菌の増殖
が顕著であることが示される。As is clear from this test, in the method of the present invention in which soybean broth is added to the basidiomycete medium and cultured, the growth of II ascomycetes is remarkable.
第１図は本発明の方法により担子菌が顕著に増殖するこ
とを示す図面である。
図中、・−・は大豆煮汁を培地に１０％（重量）添加し
た場合の多糖の生成ｆｆｉ （ｇ／β）を示す。
・−・は大豆煮汁を培地に添加しない場合の多糖の生成
ｉｉ（ｇ／／２）を示す。
○−○は大豆煮汁を培地に１０％（重■）添加′した場
合の菌糸体の生成ｆｆｉ （ｇ／ｆ）を示す。
Ｏ・−・Ｏは大豆煮汁を培地に添加しない場合の菌糸体
の生成ｉｔ （ｇ／ｊ）を示す。FIG. 1 is a drawing showing that basidiomycetes significantly proliferate by the method of the present invention. In the figure, ... indicates the polysaccharide production ffi (g/β) when 10% (weight) of soybean broth is added to the medium. .--. indicates polysaccharide production ii (g//2) when soybean broth is not added to the medium. ○ to ○ indicate mycelium production ffi (g/f) when 10% (heavy ■) of soybean broth was added to the medium. O.-.O indicates the mycelium production (g/j) when soybean broth is not added to the medium.
Claims (1)
させることを特徴とする担子菌の培養方法。1. A method for culturing basidiomycetes, which comprises inoculating basidiomycetes into a medium containing soybean broth and growing mycelia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16169384A JPS6140786A (en) | 1984-07-31 | 1984-07-31 | Method for cultivating basidiomycete |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16169384A JPS6140786A (en) | 1984-07-31 | 1984-07-31 | Method for cultivating basidiomycete |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6140786A true JPS6140786A (en) | 1986-02-27 |
Family
ID=15740064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16169384A Pending JPS6140786A (en) | 1984-07-31 | 1984-07-31 | Method for cultivating basidiomycete |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6140786A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1154022A4 (en) * | 1999-12-15 | 2004-06-09 | Amino Up Chemical Co Ltd | NOVEL SUBSTANCE FROM THE CULTURE OF A BASIDIOMYCETE, PROCESS FOR THE PRODUCTION AND USE OF THE SAME |
| EP1533371A4 (en) * | 2002-08-29 | 2005-11-23 | Mandom Corp | Culture having phenol oxidase-like activity |
| JP2019050744A (en) * | 2017-09-13 | 2019-04-04 | 有限会社プレステックス | Ganoderma mycelium culture method in soybean medium and Ganoderma mycelium health food containing soybean |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52117754A (en) * | 1976-03-31 | 1977-10-03 | Ishikawa Prefecture | Production of edible mushroom by using smothered bean soup |
| JPS5561794A (en) * | 1978-10-31 | 1980-05-09 | Fujijin Shoyu Kk | Culture of mushrooms |
-
1984
- 1984-07-31 JP JP16169384A patent/JPS6140786A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52117754A (en) * | 1976-03-31 | 1977-10-03 | Ishikawa Prefecture | Production of edible mushroom by using smothered bean soup |
| JPS5561794A (en) * | 1978-10-31 | 1980-05-09 | Fujijin Shoyu Kk | Culture of mushrooms |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1154022A4 (en) * | 1999-12-15 | 2004-06-09 | Amino Up Chemical Co Ltd | NOVEL SUBSTANCE FROM THE CULTURE OF A BASIDIOMYCETE, PROCESS FOR THE PRODUCTION AND USE OF THE SAME |
| JP4763202B2 (en) * | 1999-12-15 | 2011-08-31 | 株式会社アミノアップ化学 | Novel substance derived from basidiomycete culture, production method and use thereof |
| EP1533371A4 (en) * | 2002-08-29 | 2005-11-23 | Mandom Corp | Culture having phenol oxidase-like activity |
| US7135184B2 (en) | 2002-08-29 | 2006-11-14 | Mandom Corporation | Culture having phenol oxidase-like activity |
| CN100448988C (en) * | 2002-08-29 | 2009-01-07 | 株式会社漫丹 | Cultures with phenoloxidase-like activity |
| JP2019050744A (en) * | 2017-09-13 | 2019-04-04 | 有限会社プレステックス | Ganoderma mycelium culture method in soybean medium and Ganoderma mycelium health food containing soybean |
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