JPS6168048A - Blood bag - Google Patents
Blood bagInfo
- Publication number
- JPS6168048A JPS6168048A JP59189198A JP18919884A JPS6168048A JP S6168048 A JPS6168048 A JP S6168048A JP 59189198 A JP59189198 A JP 59189198A JP 18919884 A JP18919884 A JP 18919884A JP S6168048 A JPS6168048 A JP S6168048A
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- blood bag
- blood
- cells
- acid polymer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000008280 blood Substances 0.000 title claims description 16
- 210000004369 blood Anatomy 0.000 title claims description 16
- 150000001413 amino acids Chemical class 0.000 claims description 13
- 229920000642 polymer Polymers 0.000 claims description 11
- 235000001014 amino acid Nutrition 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 9
- 239000000463 material Substances 0.000 description 7
- 230000021164 cell adhesion Effects 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 229920001519 homopolymer Polymers 0.000 description 3
- 238000000465 moulding Methods 0.000 description 3
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- DHQUQYYPAWHGAR-UHFFFAOYSA-N dibenzyl 2-aminopentanedioate Chemical compound C=1C=CC=CC=1COC(=O)C(N)CCC(=O)OCC1=CC=CC=C1 DHQUQYYPAWHGAR-UHFFFAOYSA-N 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical class NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 238000000944 Soxhlet extraction Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 150000001509 aspartic acid derivatives Chemical class 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- YEJSPQZHMWGIGP-UHFFFAOYSA-N dl-glutamic acid dimethyl ester Natural products COC(=O)CCC(N)C(=O)OC YEJSPQZHMWGIGP-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 150000002668 lysine derivatives Chemical class 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- SEWIYICDCVPBEW-UHFFFAOYSA-N methyl glutamate Chemical compound COC(=O)C(N)CCC(O)=O SEWIYICDCVPBEW-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- -1 mybalin Chemical compound 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- UQDJGEHQDNVPGU-UHFFFAOYSA-N serine phosphoethanolamine Chemical compound [NH3+]CCOP([O-])(=O)OCC([NH3+])C([O-])=O UQDJGEHQDNVPGU-UHFFFAOYSA-N 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
Landscapes
- Medical Preparation Storing Or Oral Administration Devices (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
（ａ＞発明の技術分野
本発明は、アミノ酸重合体を内表面に有することにより
、細胞が付着しにくいことを特徴とする血液バッグに関
するものである。Detailed Description of the Invention (a> Technical Field of the Invention The present invention relates to a blood bag that is characterized by having an amino acid polymer on its inner surface, thereby making it difficult for cells to adhere to it.
血液バッグとは輸血用の血液を保存する袋状容器である
。A blood bag is a bag-like container that stores blood for transfusion.
（ｂ）従来技術の説明
従来、血液バッグは、シリコーンゴム、ポリウレタン、
ポリ塩化ビニル等の合成高分子材料を用いて作製されて
いるが、これらの材料を用いた血液バッグでは、その材
料表面に細胞が付着し、血液の組成が変化する。したが
って、血液バッグにおいては細胞の付着しない材料が求
められている。(b) Description of the prior art Conventionally, blood bags are made of silicone rubber, polyurethane,
Blood bags are manufactured using synthetic polymeric materials such as polyvinyl chloride, but in blood bags made of these materials, cells adhere to the surface of the material and the composition of the blood changes. Therefore, in blood bags, there is a need for materials to which cells do not adhere.
（Ｃ）発明の目的
本発明は上記の問題を、アミノ酸重合体を用いることに
より、細胞付着の少ない血液バッグを提供することを目
的とする。(C) Object of the Invention The object of the present invention is to solve the above problem by using an amino acid polymer to provide a blood bag with less cell adhesion.
（ｄ）発明の構成
本発明者は細胞の付着しにくい性質を有する材料につい
て種々研究を重ねたところ、アミノ酸重合体は、細胞を
著しく付着させない性質を有しており、血液バッグとし
て好適であることを見い出し、本発明を完成するに到っ
た。(d) Structure of the Invention The present inventor has conducted various studies on materials that have properties that prevent cells from adhering to them, and has found that amino acid polymers have properties that do not allow cells to adhere to a large extent and are suitable for use as blood bags. This discovery led to the completion of the present invention.
即ち、本発明の血液バッグは、アミノ酸重合体を目的と
する形状に成型して得るか、あるいはあらかじめ他の高
分子材料で目的とする形状に成型した後、その内表面に
アミノ酸重合体を塗布して得る。That is, the blood bag of the present invention can be obtained by molding an amino acid polymer into a desired shape, or by molding an amino acid polymer into a desired shape in advance using another polymeric material, and then coating the inner surface of the bag with an amino acid polymer. and get it.
本発明のアミノ酸重合体構成素材としてのアミノ酸は、
０体、Ｌ体、ラセミ体でもよく、アミノ酸として、アラ
ニン、グリシン、メチオニン、＃≠社ミバリン、グルタ
ミンＭＭＡ導体、アスパラギン酸誘導体、リジン誘導体
、オルニチン誘導体などが用いられる。アミノ酸重合体
の分子量はその皮膜が形成される程度であればよい。Amino acids as constituent materials of the amino acid polymer of the present invention are:
It may be in the 0-form, L-form, or racemic form, and the amino acids used include alanine, glycine, methionine, mybalin, glutamine MMA conductor, aspartic acid derivative, lysine derivative, ornithine derivative, and the like. The molecular weight of the amino acid polymer may be such that it forms a film.
（ｅ）発明の実施例 次に本発明を実施例によりさらに詳細に説明する。(e) Examples of the invention Next, the present invention will be explained in more detail with reference to Examples.
実施例１
グルタミン酸ベンジル単独重合体のジオキサン溶液をガ
ラス板上に流延し、風乾して皮膜（膜厚的０．０５ ｍ
１ｌｌ）を得た。この皮膜をエタノールでソックスレー
抽出を行った後、乾燥した。Example 1 A dioxane solution of benzyl glutamate homopolymer was cast onto a glass plate and air-dried to form a film (0.05 m thick).
1 liter) was obtained. This film was subjected to Soxhlet extraction with ethanol and then dried.
実施例２
グルタミン酸ベンジル単独重合体のジオキサン溶液のか
わりに、グルタミン酸メチル単独重合体のジクロルエタ
ン溶液を用いた以外は実施例１と同様にして皮膜を得た
。Example 2 A film was obtained in the same manner as in Example 1, except that a dichloroethane solution of methyl glutamate homopolymer was used instead of the dioxane solution of benzyl glutamate homopolymer.
実施例３
実施例ゴ及び実施例２で得た皮膜上で、人由来の上皮性
細胞を含む培養液（約１０万個／ｍＱ）を接触さゼたま
ま、炭酸ガス濃度５％、湿度１００％、３１℃の部屋に
静置した。１７時間後、皮膜をリン酸緩衝液でかるく洗
浄し、皮膜上に付着している細胞の量を核染色法により
定量した。比較のため、血液バッグに使用されているポ
リ塩化ビニル、標準試料として市販の細胞培養シートを
用いて、同様の細胞付着試験を行った。皮膜に付着した
細胞の量を、標準試料に付着した細胞の量で割ることに
よ一す、細胞付着率を求め、その結果を第１表に示す。Example 3 A culture solution containing human-derived epithelial cells (approximately 100,000 cells/mQ) was kept in contact with the films obtained in Example 3 and Example 2 at a carbon dioxide concentration of 5% and a humidity of 100%. %, and left standing in a room at 31°C. After 17 hours, the film was gently washed with phosphate buffer, and the amount of cells adhering to the film was quantified by nuclear staining. For comparison, a similar cell adhesion test was conducted using polyvinyl chloride used in blood bags and a commercially available cell culture sheet as a standard sample. The cell adhesion rate was determined by dividing the amount of cells attached to the film by the amount of cells attached to the standard sample, and the results are shown in Table 1.
第１表
実施例４
ガラス容器の内表面にアミノ酸重合体を塗布し、乾燥後
ガラス容器からはずし、袋状容器を得た。Table 1 Example 4 An amino acid polymer was coated on the inner surface of a glass container, and after drying, it was removed from the glass container to obtain a bag-shaped container.
（ｆ）発明の効果
本発明は以上説明したように、細胞付着の少ないことを
必要とする血液バッグにおいて、アミノ酸重合体を内表
面に成型することにより細胞の付着を抑え、かつ、この
血液バッグを任意の形状で１ｑることが可能である。(f) Effects of the Invention As explained above, the present invention, in a blood bag that requires less cell adhesion, suppresses cell adhesion by molding an amino acid polymer on the inner surface of the blood bag. It is possible to have 1q in any shape.
指定代理人 工業技術院製品科学研究所長 高橋枚司designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology Takahashi Hirayashi
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59189198A JPS6168048A (en) | 1984-09-10 | 1984-09-10 | Blood bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59189198A JPS6168048A (en) | 1984-09-10 | 1984-09-10 | Blood bag |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6168048A true JPS6168048A (en) | 1986-04-08 |
| JPH028739B2 JPH028739B2 (en) | 1990-02-27 |
Family
ID=16237159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59189198A Granted JPS6168048A (en) | 1984-09-10 | 1984-09-10 | Blood bag |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6168048A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0649341U (en) * | 1992-12-11 | 1994-07-05 | ポーラ化成工業株式会社 | Bag-shaped container |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59189197A (en) * | 1983-04-11 | 1984-10-26 | 味の素株式会社 | Detergent composition |
-
1984
- 1984-09-10 JP JP59189198A patent/JPS6168048A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59189197A (en) * | 1983-04-11 | 1984-10-26 | 味の素株式会社 | Detergent composition |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH028739B2 (en) | 1990-02-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |