JPS6182756A - Skin piercing tube - Google Patents
Skin piercing tubeInfo
- Publication number
- JPS6182756A JPS6182756A JP59205215A JP20521584A JPS6182756A JP S6182756 A JPS6182756 A JP S6182756A JP 59205215 A JP59205215 A JP 59205215A JP 20521584 A JP20521584 A JP 20521584A JP S6182756 A JPS6182756 A JP S6182756A
- Authority
- JP
- Japan
- Prior art keywords
- skin
- glutamate
- amino acid
- tube
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001577 copolymer Polymers 0.000 claims description 16
- DHQUQYYPAWHGAR-UHFFFAOYSA-N dibenzyl 2-aminopentanedioate Chemical compound C=1C=CC=CC=1COC(=O)C(N)CCC(=O)OCC1=CC=CC=C1 DHQUQYYPAWHGAR-UHFFFAOYSA-N 0.000 claims description 13
- 235000001014 amino acid Nutrition 0.000 description 13
- 210000004027 cell Anatomy 0.000 description 10
- 238000000465 moulding Methods 0.000 description 5
- 230000021164 cell adhesion Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000000149 penetrating effect Effects 0.000 description 3
- HFZKKJHBHCZXTQ-JTQLQIEISA-N (4s)-4-azaniumyl-5-oxo-5-phenylmethoxypentanoate Chemical compound OC(=O)CC[C@H](N)C(=O)OCC1=CC=CC=C1 HFZKKJHBHCZXTQ-JTQLQIEISA-N 0.000 description 2
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical compound ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- SEWIYICDCVPBEW-BYPYZUCNSA-N L-glutamate methyl ester Chemical compound COC(=O)[C@@H](N)CCC(O)=O SEWIYICDCVPBEW-BYPYZUCNSA-N 0.000 description 2
- YEJSPQZHMWGIGP-UHFFFAOYSA-N dl-glutamic acid dimethyl ester Natural products COC(=O)CCC(N)C(=O)OC YEJSPQZHMWGIGP-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- SEWIYICDCVPBEW-UHFFFAOYSA-N methyl glutamate Chemical compound COC(=O)C(N)CCC(O)=O SEWIYICDCVPBEW-UHFFFAOYSA-N 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- 239000004945 silicone rubber Substances 0.000 description 2
- OJTJKAUNOLVMDX-LBPRGKRZSA-N (2s)-6-amino-2-(phenylmethoxycarbonylamino)hexanoic acid Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)OCC1=CC=CC=C1 OJTJKAUNOLVMDX-LBPRGKRZSA-N 0.000 description 1
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical class NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 229930195714 L-glutamate Natural products 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 238000000944 Soxhlet extraction Methods 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 150000001509 aspartic acid derivatives Chemical class 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000000959 ear middle Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 150000002306 glutamic acid derivatives Chemical class 0.000 description 1
- 238000001631 haemodialysis Methods 0.000 description 1
- 230000000322 hemodialysis Effects 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 150000002668 lysine derivatives Chemical class 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
（ａ）発明の技術分野
本発明は、グルタミン酸ベンジルを含むアミノ酸共重合
体を外表面に有することにより、細胞が付着しやすいこ
とを特徴とする皮膚貫通管に関するものである。Detailed Description of the Invention (a) Technical Field of the Invention The present invention relates to a skin-penetrating tube that is characterized by having an amino acid copolymer containing benzyl glutamate on its outer surface so that cells can easily attach thereto. be.
皮Ｆｉ貫通菅とは、血液、輸液、透析液、電気などを体
外へ導出あるいは体内へ導入するために用いられる管状
物で、長期間にわたり皮膚を貫通して使用するｂのであ
る。例えば、血液透析における外シャント、カテーテル
、腹膜透析用チューブ、人工中耳のための電気コードな
どを包含するものである。A skin-penetrating tube is a tubular object used to lead blood, infusions, dialysate, electricity, etc. out of the body or into the body, and is used by penetrating the skin for a long period of time. Examples include external shunts for hemodialysis, catheters, tubes for peritoneal dialysis, electrical cords for middle ear implants, and the like.
（ｂ）従来技術の説明
従来、皮膚貫通管はシリコーンゴム、ポリウレタン、ポ
リ塩化ビニル等の合成高分子材料を用いて作製されてい
るが、これらの材料を用いた皮膚貫通管では、その材料
表面に細胞が付着しにくく、すなわち管の外表面と皮膚
の上皮組織とが密着せず、この部分で菌感染が起こる。(b) Description of the prior art Conventionally, skin-penetrating tubes have been made using synthetic polymer materials such as silicone rubber, polyurethane, and polyvinyl chloride. It is difficult for cells to adhere to the tube, which means that the outer surface of the tube and the epithelial tissue of the skin do not come into close contact, and bacterial infection occurs in this area.
したがって、皮膚貫通管においては細胞が付着する材料
が求められている。Therefore, materials to which cells can adhere are required for skin-penetrating tubes.
（Ｃ）発明の目的
本発明は上記の問題を、グルタミン酸ベンジルを含むア
ミノ酸共重合体を用いることにより、■胞付着性の多い
皮膚貫通管を提供することを目的とする。(C) Object of the Invention The object of the present invention is to solve the above-mentioned problems by using an amino acid copolymer containing benzyl glutamate, and thereby provide a skin-penetrating tube with high cell adhesion.
（ｄ）発明の構成
本発明者は細胞の付着しやすい性質を有する材料につい
て種々研究を重ねたところ、グルタミン酸ベンジルを含
むアミノ酸共重合体は、細胞を多量に付着させる性質を
有しており、皮膚貫通管として好適であることを見い出
し、本発明を完成りるに至った。(d) Structure of the Invention The present inventor has conducted various studies on materials that have properties that allow cells to easily adhere to them, and has found that an amino acid copolymer containing benzyl glutamate has the property of allowing a large amount of cells to adhere to them. The present inventors have discovered that it is suitable as a skin-penetrating tube, and have completed the present invention.
即ら、本発明の皮円量通管は、グルタミン酸ベンジルを
含むアミノ酸共重合体を目的とする管状に成型して得る
か、あるいはあらかじめ他の高分子材料で管状に成型し
た後、その外表面にグルタミン酸ベンジルを含むアミノ
酸共重合体を塗布して得る。That is, the tubular tube of the present invention can be obtained by molding an amino acid copolymer containing benzyl glutamate into a desired tubular shape, or it can be obtained by molding an amino acid copolymer containing benzyl glutamate into a tubular shape in advance, or by molding it into a tubular shape from another polymeric material and then molding the outer surface of the tube. It is obtained by applying an amino acid copolymer containing benzyl glutamate to
本発明のアミノ酸共重合体構成素材としてのグルタミン
酸ベンジル及びその共重合成分としてのアミノ酸は、０
体、Ｌ体、セラミ体でもよく、他のアミノ酸として、ア
ラニン、グリシン、メチオニン、バリン、グルタミン酸
誘導体、アスパラギン酸誘導体、リジン誘導体、オルニ
チン誘り体などが、用いられる。アミノ酸共重合体の分
子量はその皮膜が形成される程度であればよく、また共
重合体中のグルタミン酸ベンジルの含量はアミノ酸の種
類によって変わるが、１０モル％以上が好ましい。The benzyl glutamate as the constituent material of the amino acid copolymer of the present invention and the amino acid as its copolymer component are 0
Other amino acids that can be used include alanine, glycine, methionine, valine, glutamic acid derivatives, aspartic acid derivatives, lysine derivatives, ornithine derivatives, and the like. The molecular weight of the amino acid copolymer is sufficient as long as it forms a film, and the content of benzyl glutamate in the copolymer varies depending on the type of amino acid, but is preferably 10 mol % or more.
（ｅ）発明の実施例 次に本発明を実施例によりさらに詳細に説明する。(e) Examples of the invention Next, the present invention will be explained in more detail with reference to Examples.
実施例１
Ｌ−グルタミン酸メチルとＬ−グルタミン酸ベンジルと
の共重合体（グルタミン酸メチルとグルタミン酸ベンジ
ルとのモル比−１＝１）の１．２−ジクロルエタン溶液
をガラス板上に流延し、風乾して皮Ｖ（膜厚的０．０５
ｍｍ＞を得た。この皮膜をエタノールでソックスレー抽
出を行った後、乾燥した。Example 1 A 1,2-dichloroethane solution of a copolymer of methyl L-glutamate and benzyl L-glutamate (molar ratio of methyl glutamate and benzyl glutamate -1 = 1) was cast onto a glass plate and air-dried. skin V (film thickness 0.05
mm> was obtained. This film was subjected to Soxhlet extraction with ethanol and then dried.
この皮膜上で、人由来の上皮性細胞を含む培養液（約１
０万個／ｍｌ ＞と接触ざゼたまま、炭酸ガス濃度５％
、湿度１００％、３７℃の部屋に静置した。１７時間の
ち、皮膜をリン酸緩衝液でかるく洗浄し、皮膜上に付着
している細胞の量を核染色法により定量した。比較のた
め、グルタミン酸メチル単独重合体皮膜及び皮ＦＦｉ貫
通管に使用されているシリコーンゴム、標準試料として
市販の細胞培養シートを用いて、同様の細胞付着実験を
行った。皮膜に付着した細胞の儂を、標準試料に付着し
た細胞の螢で割ることにより、細胞付着率を求めた。そ
の結果を第１表に示す。On this film, a culture solution containing human-derived epithelial cells (approximately 1
00,000 pieces/ml > remains in contact with carbon dioxide concentration 5%
It was left standing in a room with 100% humidity and 37°C. After 17 hours, the film was gently washed with phosphate buffer, and the amount of cells adhering to the film was quantified by nuclear staining. For comparison, a similar cell adhesion experiment was conducted using a methyl glutamate homopolymer film, the silicone rubber used in the skin FFi penetrating tube, and a commercially available cell culture sheet as a standard sample. The cell attachment rate was determined by dividing the number of cells attached to the film by the number of cells attached to the standard sample. The results are shown in Table 1.
第 １ 表
実施例２
Ｌ−グルタミン酸メチルとＬ−グルタミン酸ペンシルと
の共重合体の１．２−ジクロルエタン溶液のかわりに、
カルボベンン゛キシーＬ−リジンとＬ−グルタミン酸ベ
ンジルとの共重合体くカルボベンゾキシリジンとグルタ
ミン酸ベンジルとのモル比−７：３）のジオキサン溶液
を用いた以外は、実施例１と同様にして、皮膜を成型し
、細胞付着実験を行った。結果を第２表に示す。Table 1 Example 2 Instead of a 1,2-dichloroethane solution of a copolymer of methyl L-glutamate and pencil L-glutamate,
A copolymer of carbobenzoxy L-lysine and benzyl L-glutamate was prepared in the same manner as in Example 1, except that a dioxane solution with a molar ratio of carbobenzoxylidine and benzyl glutamate of -7:3 was used. A film was molded and a cell adhesion experiment was conducted. The results are shown in Table 2.
第２表
実施例３
ガラス管の外表面にグルタミン酸ベンジル含有アミノ酸
共重合体を塗布し、乾燥後ガラス管からはずし、管状物
を得た。Table 2 Example 3 An amino acid copolymer containing benzyl glutamate was coated on the outer surface of a glass tube, and after drying, it was removed from the glass tube to obtain a tubular product.
（ｆ）発明の効果
本発明は以上説明したように、細胞付着性の多いことを
必要とする皮膚貫通管において、グルタミン酸ベンジル
を含むアミノ酸共重合体を外表面に成型することにより
細胞の付着を増し、がっ、この皮ＦＲ貫通管を任意の形
状で（ｑることが可能である。(f) Effects of the Invention As explained above, the present invention improves the adhesion of cells by molding an amino acid copolymer containing benzyl glutamate on the outer surface of a skin-penetrating tube that requires high cell adhesion. It is possible to make this skin FR penetrating tube into any shape.
指定代理人 工業技術院製品科学研究所長 高橋孜司designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology Keiji Takahashi
Claims (1)
外表面に有する皮膚貫通管(1) Skin-penetrating tube with an amino acid copolymer containing benzyl glutamate on the outer surface
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59205215A JPS6182756A (en) | 1984-09-28 | 1984-09-28 | Skin piercing tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59205215A JPS6182756A (en) | 1984-09-28 | 1984-09-28 | Skin piercing tube |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6182756A true JPS6182756A (en) | 1986-04-26 |
| JPS644471B2 JPS644471B2 (en) | 1989-01-25 |
Family
ID=16503308
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59205215A Granted JPS6182756A (en) | 1984-09-28 | 1984-09-28 | Skin piercing tube |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6182756A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0428681U (en) * | 1990-06-29 | 1992-03-06 |
-
1984
- 1984-09-28 JP JP59205215A patent/JPS6182756A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS644471B2 (en) | 1989-01-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |