JPS6170979A - Cultivation of marine chlorella - Google Patents
Cultivation of marine chlorellaInfo
- Publication number
- JPS6170979A JPS6170979A JP59190468A JP19046884A JPS6170979A JP S6170979 A JPS6170979 A JP S6170979A JP 59190468 A JP59190468 A JP 59190468A JP 19046884 A JP19046884 A JP 19046884A JP S6170979 A JPS6170979 A JP S6170979A
- Authority
- JP
- Japan
- Prior art keywords
- chlorella
- marine chlorella
- marine
- salt concentration
- culture solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〈産業上の利用分野〉
本発明は海産クロレラを開放条件下で培養しても他の生
物等による汚染の少ない培養法に係る。DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to a method for culturing marine chlorella under open conditions with less contamination by other organisms.
〈従来の技術〉
海産クロレラは古くから養魚飼料用に利用されてきたが
、屋外池での培養であるためラン環、ケイ環等の淡水性
植物プランクトン、原生動物である動物プランクトン、
バクテリア等による汚染が避けられず、今日まで、これ
を解決する有効な手段は見出されていない。培養を密閉
条件下で行えば、これらの汚染が避けられる可能性はあ
るが、かかる条件を満足させるには装置、機材、に高コ
ストを要するという難点がある。<Conventional technology> Marine chlorella has been used for fish feed for a long time, but because it is cultivated in outdoor ponds, it is not used for freshwater phytoplankton such as orchid ring and keel ring, zooplankton which is a protozoan,
Contamination by bacteria and the like is unavoidable, and to date, no effective means to solve this problem has been found. If the culture is carried out under closed conditions, it is possible to avoid these contaminations, but there is a drawback in that high costs are required for equipment and equipment to satisfy such conditions.
〈発明が解決しようと゛する問題点〉
本□発明の目的はコストの安い開放条件下での培養であ
っても上記のよ□うな汚染の少ない海産クロレラの培養
法を提供することにある。<Problems to be Solved by the Invention> The purpose of the present invention is to provide a method for cultivating marine chlorella that is inexpensive and causes less contamination as described above even when cultured under open conditions.
く問題点を解決するための手段〉
本発萌者らは鋭意研究の結果、培養液をある特定の条件
に設定すると、上記の目的が達成されることを見い出し
本発明に到達した。Means for Solving the Problems As a result of intensive research, the present inventors have discovered that the above object can be achieved by setting the culture solution under certain specific conditions, and have arrived at the present invention.
即ち本発明は海産クロレラを開放条件十で培養するに際
し、炭素源としてＮａ、Ｃｏう又はＮａｕｃｏｓを用い
、培養液のｐ Ｈを８．０〜１０．０とし、培養液中の
食塩濃度を０．８〜３．０％とすることを特徴とする海
産クロレラの培養方法である。That is, in the present invention, when culturing marine chlorella under open conditions, Na, Co, or Naucos is used as a carbon source, the pH of the culture solution is set to 8.0 to 10.0, and the salt concentration in the culture solution is set to 0. .8 to 3.0%.
本発明でいう海産クロレラとは、特に種属などを限定す
るものではないが、通常クロレラ・ミニュティシマ（Ｃ
ｈｌｏｒｅｌｌａ ｎ＋ｉｕｎｔｉｓｓｉｍａ ） 、
クロレラ・ブルガリスＣＣ，ｖｕｌｇａｒｉｓ）等に分
類されているものである。The marine chlorella referred to in the present invention is not particularly limited to species or genus, but is usually Chlorella minutisima (C
hlorella n+untissima),
It is classified as Chlorella vulgaris CC, vulgaris), etc.
海産クロレラは炭素源として酢酸、グルコースを用いて
も生育が可能であるが、バクテリ゛アもこれらを資化で
き、しかもクロレラより生育速度がはやいため、上記の
物質を炭素源として使用すると、バクテリアが大量に繁
殖し、クロレラが汚染される。これに対しＮ ａ、 Ｇ
Ｏ’ｚ 、ＮａＨＣ０３はバクテリアおよび淡水クロ
レラが資化できないため、炭素源としてこれらを用いれ
ばバクテリアおよび淡水クロレラの汚染が避けられる。Marine chlorella can grow using acetic acid and glucose as carbon sources, but bacteria can also assimilate these, and their growth rate is faster than chlorella. Proliferates in large numbers and contaminates chlorella. On the other hand, N a, G
Since O'z and NaHC03 cannot be assimilated by bacteria and freshwater chlorella, contamination of bacteria and freshwater chlorella can be avoided by using them as carbon sources.
培養液が弱アルカリ性であると淡水プランクトンの生育
が抑制されるが、ｐ Ｈが１０．０を超えると海産クロ
レラが凝集を起こし沈降して生育が阻害される。かかる
知見に基づいて本発明では培゛養液のｐ Ｈを８．０〜
１Ｏ００とするものであり、この条件下であれば、淡水
プランクトンの汚染を効果的に防止しつつ海産クロレラ
の生育が効果的に行われる。If the culture solution is slightly alkaline, the growth of freshwater plankton will be suppressed, but if the pH exceeds 10.0, marine chlorella will coagulate and settle, inhibiting growth. Based on this knowledge, in the present invention, the pH of the culture solution is set to 8.0-8.0.
1000, and under these conditions, marine chlorella can grow effectively while effectively preventing freshwater plankton contamination.
培養液中の食塩濃度が０．８％を超えると、淡水性植物
プランクトン（ラン藻など）および動物コ°う′クト７
（”帖ゲＪｓ’ｉ・ツリ”ネ”シなど）が死滅する。−
万全塩濃度が３．０％を超えると海産クロレラの生育が
阻害される。以上から本発明では培養液中の食塩濃度を
０．８〜３．０％としたものである。If the salt concentration in the culture solution exceeds 0.8%, freshwater phytoplankton (such as blue-green algae) and animal insects
("choge Js'i, tsuri"ne"shi, etc.) will die.-
If the total salt concentration exceeds 3.0%, the growth of marine chlorella will be inhibited. From the above, in the present invention, the salt concentration in the culture solution is set to 0.8 to 3.0%.
実施例１
開放攪拌方式で炭素源としてＮａＨＣＯ３を３００ｇ／
を用い、他に窒素、リン酸、カリウムを含りｐＨ＝８．
５の一養液中で食塩濃度Ｏ，２％の条件下に海産クロレ
ラの培養を行った。海産クロレラがＰ ＣＶ （ｐａｃ
ｋｅｄ ｃｅｌｌ ｖｏｌｕｍｅ湿細胞濃度）＝２．０
まで生育した段階でカラヒゲムシが大量に発生し、顕微
鏡で観察したところ、海産クロレラを捕食し、６時間後
にはＰＣＶ＝０．１まで減少した。そこで食塩濃度を増
加させたところ０．７５％ではカラヒゲムシが運動性を
存し捕食活動が認められたが、食塩濃度０．８％の条件
下では運動性を失ない、球形の細胞形態となり増殖しな
かった。この結果、海産クロレラは順調に生育し、他の
生物による汚染は認められなかった。Example 1 300g/NaHCO3 was added as a carbon source using an open stirring method.
Also contains nitrogen, phosphoric acid, and potassium, and has a pH of 8.
Marine chlorella was cultured in a nutrient solution of No. 5 under conditions of a salt concentration of O and 2%. Marine chlorella has a P CV (pac
ked cell volume (wet cell concentration) = 2.0
When the chlorella reached the stage of growth, a large number of brown beetles appeared, and when observed under a microscope, they preyed on marine chlorella, and the PCV decreased to 0.1 after 6 hours. When the salt concentration was increased, it was found that at 0.75%, the beetles remained motile and exhibited predation activity, but at a salt concentration of 0.8%, they remained motile and took on a spherical cell shape and proliferated. I didn't. As a result, marine chlorella grew smoothly and no contamination by other organisms was observed.
実施例２
開放攪拌方式で炭素、源としてＮ、ａＨＣＯ３を３００
ｇ／を用い、他に窒素、す：ン酸、カリウム、を、含む
ｐＨ＝７．０の培養液中で食塩濃度０．２％の条件下に
海産り０レラの培養を行−た・海産りＯｌｚ、ｊ、カフ
ｐＣＶ＝２．．０まで生育した段階でミ、ジンコが発生
〜した。そこで水酸化ナトリウ十を坤えてｐｎ＝ ９．
５としたところミジンコの運動性が低下し、さらに。Example 2 Carbon, N as a source, aHCO3 at 300% by open stirring method
The sea-breeding Orera was cultured using a culture solution with a pH of 7.0 that also contained nitrogen, sulfuric acid, and potassium at a salt concentration of 0.2%. Marine Olz, j, cuff pCV=2. ．． At the stage when the seedlings grew to 0, worms and jinko appeared. So I added 100% sodium hydroxide and pn=9.
When it was set to 5, the motility of Daphnia decreased.
食塩濃度を１．０％とし−たところ、運動性を失った。When the salt concentration was set to 1.0%, motility was lost.
この結果、海産クロレラが正常に増殖した。As a result, marine chlorella grew normally.
なお培養液のｐ Ｈを１０．５としたとこ、ろ海産クロ
レラの凝集が始まり、生育が停止した。Note that when the pH of the culture solution was set to 10.5, aggregation of Chlorella from the sea began and growth stopped.
実施例３ −
開放攪拌法式て炭素源とシで酢酸を３００ ｇ／を用い
、他に窒素、リン酸、カリウムを含むｐＨ＝９．０の培
養液中で食塩濃度Ｏ１，２％の条件下に海産クロレラの
培養を行った。、海産クロレラがＰＣＶ＝１．５まで生
育した段階でバクテリアが大量に発生した。Example 3 - Using an open stirring method with 300 g of acetic acid as a carbon source and a culture solution with a pH of 9.0 containing nitrogen, phosphoric acid, and potassium at a salt concentration of 1.2%. We cultivated marine chlorella. When marine chlorella grew to a PCV of 1.5, a large amount of bacteria appeared.
これに対し炭素源としてＮａ）Ｃｏｔを３００ｇ／を用
い、他に窒素、リン酸、カリウムを含むｐＨ＝９．０の
培養液中で食塩濃度２．０％の条件下に海産クロレラの
培養を行ったところ、他の生物による汚染もなく順調に
生育した。On the other hand, marine chlorella was cultured using 300 g of Na) Cot as a carbon source and a salt concentration of 2.0% in a culture solution with a pH of 9.0 that also contained nitrogen, phosphoric acid, and potassium. When we conducted the experiment, we found that the plants were growing smoothly and were not contaminated by other organisms.
〈発明９効果〉
本発明によれば、開放条件下でも汚染の少ない海産クロ
レラや培養法が提供され、低コストで、効、率よく海産
クロレラを生産することができる。<Effects of the Invention 9> According to the present invention, marine chlorella and a cultivation method that are less contaminated even under open conditions are provided, and marine chlorella can be efficiently and efficiently produced at low cost.
特許出願人 日清製油株式会社
手続補正書（自発）
昭和ｎｍ１０月７８日
特許庁長官 ′志 賀 学 殿
１、事件の表示
昭和５９年特許願第１９０４６８号
２、発明の名称
海産クロレラの培養方法
３、補正をする者
事件との関係 特許出願人
住 所 ■２２１神奈川県横浜市神奈川区千若町１
−５名 称 日清製油株式会社 研究断電 話
０４５ （４６１）０１８１７で：＼
４、補正の対象
明細書の特許請求の範囲の欄
５、補正の内容
（１） 特許請求の範囲を別紙のとおり訂正する。Patent applicant: Nisshin Oil Co., Ltd. Procedural amendment (voluntary) Showa nm October 78, 1989 Director-General of the Patent Office Manabu Shiga 1, Indication of the case 1982 Patent Application No. 190468 2, Name of the invention Method for culturing marine chlorella 3. Relationship with the case of the person making the amendment Patent applicant address ■221 1 Chiwaka-cho, Kanagawa-ku, Yokohama-shi, Kanagawa Prefecture
-5 name Nisshin Oil Co., Ltd. Research call outage
045 (461) At 01817:\ 4. Claims column 5 of the specification to be amended, Contents of the amendment (1) The claims are corrected as shown in the attached sheet.
特許請求の範囲
海産クロレラを開放条件下で培養するに際し、炭素源と
してｐＪ ａ ｚ ＣＯ３又はＮ ａ ＨＣＯ３を用い
、培養液のｐＨを８．０〜１０．０とし、培養液中の食
塩濃度を０．８〜３．０％とすることを特徴とする海産
クロレラの培養方法。Claims: When culturing marine chlorella under open conditions, pJ az CO3 or Na HCO3 is used as a carbon source, the pH of the culture solution is set to 8.0 to 10.0, and the salt concentration in the culture solution is A method for culturing marine chlorella, characterized in that the concentration is 0.8 to 3.0%.
Claims (1)
してＮａ＿２ＣＯ＿３又はＮａＨＣＯ＿３を用い、培養
液のｐＨを８．０〜１０．０とし、培養液中の食塩濃度
を８．０〜３．０％とすることを特徴とする海産クロレ
ラの培養方法。When culturing marine chlorella under open conditions, Na_2CO_3 or NaHCO_3 is used as a carbon source, the pH of the culture solution is set to 8.0 to 10.0, and the salt concentration in the culture solution is set to 8.0 to 3.0%. A method for culturing marine chlorella, characterized by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59190468A JPS6170979A (en) | 1984-09-13 | 1984-09-13 | Cultivation of marine chlorella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59190468A JPS6170979A (en) | 1984-09-13 | 1984-09-13 | Cultivation of marine chlorella |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6170979A true JPS6170979A (en) | 1986-04-11 |
| JPS6324672B2 JPS6324672B2 (en) | 1988-05-21 |
Family
ID=16258613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59190468A Granted JPS6170979A (en) | 1984-09-13 | 1984-09-13 | Cultivation of marine chlorella |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6170979A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009117743A3 (en) * | 2008-03-21 | 2009-12-23 | University Of Washington | Novel chrysochromulina species, methods and media therefor, and products derived therefrom |
| JP2010215264A (en) * | 2009-03-17 | 2010-09-30 | Ishimura Yoshinosuke | Packaging container |
| JP2020110086A (en) * | 2019-01-11 | 2020-07-27 | 三菱重工機械システム株式会社 | Algae culture system |
-
1984
- 1984-09-13 JP JP59190468A patent/JPS6170979A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009117743A3 (en) * | 2008-03-21 | 2009-12-23 | University Of Washington | Novel chrysochromulina species, methods and media therefor, and products derived therefrom |
| JP2010215264A (en) * | 2009-03-17 | 2010-09-30 | Ishimura Yoshinosuke | Packaging container |
| JP2020110086A (en) * | 2019-01-11 | 2020-07-27 | 三菱重工機械システム株式会社 | Algae culture system |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6324672B2 (en) | 1988-05-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |