JPS6192584A - Production of methyl formate - Google Patents
Production of methyl formateInfo
- Publication number
- JPS6192584A JPS6192584A JP21424884A JP21424884A JPS6192584A JP S6192584 A JPS6192584 A JP S6192584A JP 21424884 A JP21424884 A JP 21424884A JP 21424884 A JP21424884 A JP 21424884A JP S6192584 A JPS6192584 A JP S6192584A
- Authority
- JP
- Japan
- Prior art keywords
- methyl formate
- medium
- carbon source
- ch3oh
- collecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- TZIHFWKZFHZASV-UHFFFAOYSA-N methyl formate Chemical compound COC=O TZIHFWKZFHZASV-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 238000004519 manufacturing process Methods 0.000 title claims description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 23
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 7
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims abstract description 6
- 244000005700 microbiome Species 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 150000003863 ammonium salts Chemical class 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 238000000746 purification Methods 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 229910019142 PO4 Inorganic materials 0.000 abstract description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 235000021317 phosphate Nutrition 0.000 abstract description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 abstract description 2
- 230000000284 resting effect Effects 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Natural products C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 abstract 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 abstract 1
- 241000222124 [Candida] boidinii Species 0.000 abstract 1
- 150000002894 organic compounds Chemical class 0.000 abstract 1
- 230000034303 cell budding Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 241000761456 Nops Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- 229960004172 pyridoxine hydrochloride Drugs 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】 産業上の利用分野 本発明はギ酸メチルの製造方法に関する。[Detailed description of the invention] Industrial applications The present invention relates to a method for producing methyl formate.
発明の目的
本発明者らは、メタノールを炭素源とするギ酸メチルの
製造方法について種々検討した結果、カンテイダ属に属
する微生物がメタノールをギ酸メチルに変侠する能力を
有することを見出し、本発明に到達した。Purpose of the Invention The present inventors have investigated various methods for producing methyl formate using methanol as a carbon source, and have discovered that microorganisms belonging to the genus Cantida have the ability to convert methanol into methyl formate. Reached.
すなわち、本発明の一次旨は、カンデイダ属に属し、ギ
酸メチルを生産する能力を■する微生物ケ、炭素源とし
てメタノールを使用して増養し、培養物からギ酸メチル
を得ることを特徴とするギ酸メチルの製造方法にある。That is, the primary gist of the present invention is that a microorganism belonging to the genus Candeida and having the ability to produce methyl formate is cultivated using methanol as a carbon source to obtain methyl formate from the culture. In the method for producing methyl formate.
（発明の構成） 以下、本発明を詳細ＶｃＢ！Ｉ？、明する。(Structure of the invention) The present invention will be explained in detail below. I? , reveal.
本発明において便用される微生ｖｌＪはカンデイダ（Ｃ
ａｎｄｉｄａ ）属に属し、ギばメチルを生産する能力
ｔ−ｉするものであり、友とえは、カンデイダ・ボイデ
イニイ（Ｃａｎｄｉｄａ ｂｏｌｄｌｎｉｉ ） Ａ
−／９／７−Ｊ（微工研菌寄第７１ｒｊ号）が挙げられ
る・この臼株は、土壌より分離されたものであり、その
医学的性状は次の通りである。The microorganism vlJ conveniently used in the present invention is Candida (C
It belongs to the genus A. andida and has the ability to produce methyl.
-/9/7-J (Feikoken Bacteria No. 71rj) - This mortar strain was isolated from soil, and its medical properties are as follows.
■ 各培地における生育状態
／）ＹＭｇｌＬ体培地、ＪＯＣ，３日商の生丼状悪Ｏ
生育は良好で、培養液面に環状の皮膜が形成される。培
養液紙部に沈誠を生ずる。■Growth status in each medium/)YMglL body medium, JOC, 3 Nissho's raw bowl-shaped growth O Growth is good, and a ring-shaped film is formed on the surface of the culture solution. Sinking occurs in the paper part of the culture solution.
コ）ＹＭ平板寒天上の性質
３０Ｃ１３日間の培養で、生付は良好、コロニーは、バ
ター質、白色を呈する◎コロニ二表面は平滑：周縁は全
線〜やや波状；隆起状ノぷは台状；輝光状の光沢を呈す
る。j) Properties on YM plate agar 30C After 13 days of culture, the growth is good, and the colonies are buttery and white in color. ◎ Colonial surface is smooth; periphery is entirely lined to slightly wavy; raised nops are plate-like; It exhibits a luminous luster.
細胞形感は、卵形〜悄−円形、大きさ
一一／ ０．Ｊ Ｘ八、？ −Ｊ、Ｊ （平均３．６×
コ、ｓ ）μｍ 増１ｄ昧式ｔま多・ネ出芽、偽閉糸を
形成する。子のう胞子は形成されない。The cell shape is oval to slightly round, and the size is 11/0. JX8,? -J, J (average 3.6×
ko, s) μm increase in size and form budding, forming pseudo-occlusal filaments. Ascospores are not formed.
Ｊ） ／、！ｒ％メタノール含有液体培地、Ｊ ＯＣ
。J) /,! liquid medium containing r% methanol, JOC
.
３日間の生ｄ状態・
生胃は良好で坩誉液圓に反涙は殆ど形成さ１℃ない・培
倭ｅ、社部に少量の沈謙が形成される。After 3 days, the raw stomach is in good condition, and there is almost no anti-lacrimal formation in the bulge liquid, less than 1°C.
川 生理的性質
■ 生首の範囲 ｐＨ１〜？
温度７０〜ＪＯＣ
、？７ｔｌｌ、で生首せず
■ 岨酸温の同化：あり
■ ゼラチン叔化龍：なし
■ デンプン端似物貞の生成：なし
■ ５０％グルコース上での生Ｗ（ＹＭ）：なしくり
ＮｔＣＩＩｌＩＩｆ性：ｇＳ−９％■ メタノール貧化
１土：あり
■ ペクチン簀化社：あり
■ ビタミン要釆註：ビオテン
リ 炭素源の利用性（あり：＋、なし：４ｖ出芽によっ
て増殖する、子のう胞子を形成しない、ことから、ロダ
ーの覧ザ・イースト〃（２版・ｔｑｑｏ ）に記載され
ていクヵンディダ（Ｃａｎｄｉｄａ ） Ａｖ４に帰氏
することが判明した。River Physiological properties ■ Range of severed neck pH 1~? Temperature 70~JOC,? 7tll, no decapitation ■ Assimilation of lactate temperature: Yes ■ Gelatin Shuhualong: None ■ Formation of starch end analogues: None ■ Raw W (YM) on 50% glucose: No NtCIIlIIf property: gS-9% ■ Methanol-poor 1 soil: Yes ■ Pectin Kankasha: Yes ■ Vitamin notes: Biotenry Carbon source availability (Yes: +, No: 4v Proliferates by budding, does not form ascospores) From this, it was revealed that he returned to Candida Av4, which was written in Roder's View of the East (2nd edition, tqqo).
バ 涌の同定
一ル貸化１抽としてｓ ４Ｍを認めている・（Ｃ，ｂｏ
ｉｄｉｎＮ、 Ｃ，ｃａｒｌｏａｉｌｉｇｎｉｃｏｌａ
、 Ｃ６１ｉｐｏｌｙｔｌｃａ。s 4M is recognized as the 1st draw for the identification of Ba Waku (C, bo
idinN, C, carloailignicola
, C61ipolytlca.
Ｃ，５ｕｃｃｉｐｈｊｌａ、 Ｃ，ｕｔｌｌｉａ ）。C, 5ucciphjla, C, utllia).
こ才りらのＳ（虫は、据−の発畔目己パターン及び糖胡
の資化性パターンによって生’ＩＣ１ｆｆｌｔ別されて
いる。Insects are classified according to their budding pattern and assimilation pattern.
本［抹（Ａ−／？／７−Ｊ）ＯＭａｍ（Ｄ発ａ性試躾及
び上記の炭素源の資化性パターンはロダ−（ｌｙ７ｏ）
のザ・イースト及びリーと駒形（／９１０）Ｋよって５
己載されているカンディダ・ボイディニイ（Ｃａｎｄｌ
ｄａ ｂｏｌｄｌｎｉｌ ）の諸性質とよく合致する。This book [Maku(A-/?/7-J)
The East and Lee and Komagata (/910) K by 5
Candida Voidiny (Candl)
da boldlnil).
よって本菌株（Ａ−ｔ ｑ ｔ ７−、？ ） ｆ、ｉ
カンディダーボイディニイ（Ｃａｎｄｌｄａ ｂｏｌｄ
ｌｎｉｌ ）と同定された□また本菌株（Ａ−／ ９
／ ７−Ｊ ）とカンデイダ・ボイディニイ（Ｃａｎｄ
ｌｄａ ｂｏｉｄｎｉｌ ）のＡｕｔｈｅｎｔｌｃ ５
ｔｒａｉｎ、 （Ｉ Ａ Ｍ ／ ２２ ｌｘ ９株）
の形態イダ・ボイディニイ（ＩＡＭ ｌ−２ａ９）のそ
れらともよく一致した。Therefore, this strain (A-t q t 7-,?) f, i
Candlda bold
□Also, this strain (A-/9
/ 7-J) and Candeida Voidiny (Cand
Authentic 5 by lda boidnil)
train, (IAM/22 lx 9 shares)
It also matched well with those of Ida boidinii (IAM l-2a9).
特に制限されない・
炭素源としてｔユ、メタノール以外に、独々の炭水化物
、有機酸各音さらに冷加してもよく、窒素源としては、
有機アンモニウム塩、無機アンモニウム塩、尿素等を用
いることができる６また、必要に応じ、無磯物として各
性リン酸塩、（ｉｉｆＵ塩等を使用することができ、必
要に応じ各１肩４幾栄誉物全添加することもできる。There are no particular restrictions.In addition to tYU and methanol, carbon sources may include individual carbohydrates and organic acids, which may be further cooled.As nitrogen sources,
Organic ammonium salts, inorganic ammonium salts, urea, etc. can be used.6 In addition, various phosphates, (iifU salts, etc.) can be used as non-isolated substances, and if necessary, one shoulder of each can be used. You can also add any number of ingredients.
２＋！ｉ誉は、通常／コ時間〜／４（日間程度、好気的
条住ドに行なわれる。培地のｐＨはダー１０゜温度はユ
Ｏ−ダＯ℃程展から選ばれる。2+! Incubation is usually carried out in an aerobic environment for about 4 hours to 4 days.The pH of the culture medium is selected from 10°C and 10°C.
キ１反メチルの土圧に際しては、増泡菌体、休止１体の
いずれをも用いることができ／）。For the earth pressure of Ki1 anti-methyl, both foamed bacterial cells and resting bacterial cells can be used.
堵Ｊｖ窃からギ１反メチルの採取、精製に除しては、一
般ＶｃＭ峻化合物の採取、棺夷に用いられている方法全
採用することができる。For the collection and purification of G1 anti-methyl from the JV theft, all methods used for the collection and purification of general VcM compounds can be employed.
（夷Ｉ山ｔ２リ ） 以下、実施列により、本発明をさらに説明する。(Yiyama t2li) The present invention will be further explained below with reference to examples.
メタノールｔｌＯｆ、ＮＨ，ＣＬダ？、 Ｋ２ＨＰＯ４
／ ？。Methanol tlOf, NH, CL da? , K2HPO4
/ ? .
Ｎａ１ＨＰＯ４／ デ、Ｍｇ５Ｏ，・りＨ２Ｏ０，３
ｆ、塩酸ピリドキシンｔｎｙ、ビオチンｏ、ｏｌｒｔｕ
；）、塩ばテアミ７／〜、リボフラビン／ｒｎ９、ｆ）
−パントテン眩ナトリウＡ ／ ｔｎ９、來＋ＲＯ，ｏ
ｉ ｔｎり、ｆ’ｅｓＯ４” ７ Ｈ２Ｏ１ｒｎ９、
ＺｎＳＯ４＠？Ｈ１０／ ＩＩＩ！／、ＣｕＳＯ４”ｔ
ｎ２００．／ ｔｎ９、菌した。天然の土壌から分離し
たカンデイダ・ポイデイニイＡ−／９／７−ｊ困のチ＋
面培養（３０℃、３〜６日ｒｉｉＪ ）―の一白金井を
上記コルベンに接種し、ｉｉミコ仮／分の在仮振とう磯
でＪＯＣＪ日間培養を行ない、この培養物！； Ｏｒｒ
ｌよりギ醒メチルコｌｌ１１７を得た。Na1HPO4/ De, Mg5O, ・H2O0,3
f, pyridoxine hydrochloride tny, biotin o, olrtu
;), Shiobateami7/~, Riboflavin/rn9, f)
- Pantoten Maji Natoriu A / tn9, come + RO, o
i tnri, f'esO4” 7 H2O1rn9,
ZnSO4@? H10/III! /,CuSO4”t
n200. /tn9, bacterium. Candida poideinii A-/9/7-j isolated from natural soil
Surface culture (30°C, 3 to 6 days) - Ichirokane was inoculated into the above-mentioned Kolben, cultured for 1 day at JOCJ on a shaking rock of 2 Mikokari/min, and this culture! ;Orr
117 was obtained from the reaction mixture.
Claims (1)
チルを生産する能力を有する微生物を、炭素源としてメ
タノールを使用して培養し、培養物からギ酸メチルを得
ることを特徴とするギ酸メチルの製造方法。(1) A method for producing methyl formate, which comprises culturing a microorganism belonging to the Candida genus and having the ability to produce methyl formate using methanol as a carbon source, and obtaining methyl formate from the culture. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21424884A JPS6192584A (en) | 1984-10-15 | 1984-10-15 | Production of methyl formate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21424884A JPS6192584A (en) | 1984-10-15 | 1984-10-15 | Production of methyl formate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6192584A true JPS6192584A (en) | 1986-05-10 |
| JPH0424989B2 JPH0424989B2 (en) | 1992-04-28 |
Family
ID=16652621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21424884A Granted JPS6192584A (en) | 1984-10-15 | 1984-10-15 | Production of methyl formate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6192584A (en) |
-
1984
- 1984-10-15 JP JP21424884A patent/JPS6192584A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0424989B2 (en) | 1992-04-28 |
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| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |