JPS6192583A - Production of propionic acid - Google Patents
Production of propionic acidInfo
- Publication number
- JPS6192583A JPS6192583A JP21424984A JP21424984A JPS6192583A JP S6192583 A JPS6192583 A JP S6192583A JP 21424984 A JP21424984 A JP 21424984A JP 21424984 A JP21424984 A JP 21424984A JP S6192583 A JPS6192583 A JP S6192583A
- Authority
- JP
- Japan
- Prior art keywords
- propionic acid
- carbon source
- medium
- candida
- ch3oh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 235000019260 propionic acid Nutrition 0.000 title claims abstract description 13
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 title claims abstract description 13
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 26
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims abstract description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 9
- 244000005700 microbiome Species 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 241000222124 [Candida] boidinii Species 0.000 abstract description 3
- 150000003863 ammonium salts Chemical class 0.000 abstract description 3
- 229910019142 PO4 Inorganic materials 0.000 abstract description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 235000021317 phosphate Nutrition 0.000 abstract description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Natural products C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 abstract 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 150000002894 organic compounds Chemical class 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 2
- 230000034303 cell budding Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 210000003127 knee Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 241001028048 Nicola Species 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】 （産業上の利用分野） 本発明はプロピオン酸の製造方法に関する。[Detailed description of the invention] (Industrial application field) The present invention relates to a method for producing propionic acid.
（発明の目的）
本発明者らは、メタノールを炭素源とするプロピオン酸
の製造方法について種々検討した結果、カンデイダＩＡ
Ｋ属する微生物がメタノールをプロピオン酸に変換する
能力を有することを見出し、本発明に到達した。(Object of the Invention) As a result of various studies on the production method of propionic acid using methanol as a carbon source, the present inventors discovered that Candida IA
The present invention was achieved based on the discovery that microorganisms belonging to the K family have the ability to convert methanol to propionic acid.
すなわち、本発明の要旨は、カンデイダ属に属し、プロ
ピオン酸を生産する能力を有する微生物を、炭素源とし
てメタノールを使用して培養し、培養物からプロピオン
酸を得ることを特徴とするプロピオン酸の製造方法にあ
る。That is, the gist of the present invention is to obtain propionic acid by culturing a microorganism belonging to the genus Candida and having the ability to produce propionic acid using methanol as a carbon source, and obtaining propionic acid from the culture. It's in the manufacturing method.
（発明の構成） 以下、本発明の詳細な説明する。(Structure of the invention) The present invention will be explained in detail below.
本発明において使用される微生物はカンデイダ（０ａｎ
ｄ１ｄａ ）属に属し、プロピオン酸を生産する能力を
有するものであり、たとえば、カンデイダ ボイデイニ
イ（Ｃａｎｄｉｄａ ｂｏｉｄｉｎｉｉ ）Ａ−／ ？
／ ７−．７ （微工研菌寄第２ｇ！３号）が挙げら
れる。The microorganism used in the present invention is Candida (0an
d1da) and has the ability to produce propionic acid, such as Candida boidinii (Candida boidinii) A-/?
/ 7-. 7 (Feikoken Bakuyori No. 2g! No. 3) is mentioned.
この菌株は、土壌より分離されたものであり、その菌学
的性状は次の通りである。This strain was isolated from soil, and its mycological properties are as follows.
■ 各培地における生育状態 ／）ＹＭ液体培地、ＪＯ′Ｇ、３日間の生育状態。■ Growth status in each medium /) YM liquid medium, JO'G, growth condition for 3 days.
生育は良好で培養液面に環状の皮膜が形成される。培養
液底部に沈澱を生ずる。Growth is good and a ring-shaped film is formed on the surface of the culture solution. A precipitate forms at the bottom of the culture solution.
リ ＹＭ平板寒天上の性質 ３０℃、３日間の培養で、生育は良好。Properties on YM flat plate agar Growth was good after culturing at 30°C for 3 days.
コロニーは、バター質、白色を呈する。Colonies are buttery and white in color.
コロニー表面は平滑；周縁は金縁〜やや波状、隆起状態
は台状；輝光状の光沢を呈する。細胞形態は、卵形〜楕
円形、大きさＪ−１０，ＪＸ／Ｊ −、？、、？ （平
均ＪＪ Ｘｊ、ｔ ）μｍ１増殖様式は多極出芽、偽菌
糸を形成する。The colony surface is smooth; the periphery is golden to slightly wavy, and the ridges are table-like; exhibiting a luminous luster. Cell morphology is oval to oval, size J-10, JX/J-, ? ,,? (Average JJ Xj,t) μm1 growth mode forms multipolar budding, pseudohyphae.
子のう胞子は形成されない。Ascospores are not formed.
３） ／、ｊチメタノール含有液体培地、３０℃３日
間の生育状態。3) /, j Thimethanol-containing liquid medium, growth condition at 30°C for 3 days.
生育は良好で培養液面に皮膜は殆ど形成されない。培養
液底部に夕景の沈澱が形成される。Growth is good and almost no film is formed on the surface of the culture solution. A precipitate appears at the bottom of the culture solution.
■ 生理的性質 ■生育の範囲 ｐＨ−〜？ 温度 ｉｏ−％−ＪＯ℃ ３７℃で生育せ ず。■ Physiological properties ■Growth range pH-~? Temperature io-%-JO℃ Grow at 37℃ figure.
■ 硝酸塩の同化 ：め シ
■ ゼラチン液化能：な し
■ Ｎａ０Ｊ 耐性 二を一タチ
■ メタノール資化性：あ シ
■ ペクチン資化性：あ リ
■ ビタミン要求性：ピオチン
パントテ／酸−〇ａ
［相］ 発酵試験 （発酵性あり：＋１発醇性冷しニー
）
■ 炭素源の利用性（あシ：＋、なしニー）■ 属レベ
ルの同定
本菌株（Ａ−／９／７−、？）は、カロチノイド色素を
生産しない、偽菌糸を常に形成する、多極出芽によって
増殖する、子のり胞子を形成しないことからロダーの１
ザ・イースト′（１版・／９７０）に記載されているカ
ンデイダ（０ａｎｄｉｉａ属）に帰属することが判明し
た。■ Assimilation of nitrate: Meshi ■ Gelatin liquefaction ability: None ■ Na0J resistance Two to one ■ Methanol assimilation ability: Yes ■ Pectin assimilation ability: Yes ■ Vitamin requirement: Piotin pantote/acid-○a [phase ] Fermentation test (fermentability: +1 simmering cold knee) ■ Carbon source utilization (reed: +, no knee) ■ Identification at the genus level This strain (A-/9/7-, ?) is , does not produce carotenoid pigments, always forms pseudohyphae, reproduces by multipolar budding, and does not form ascospores;
It was found that it belongs to Candida (genus Oandiia), which is described in The East' (1st edition /970).
■ 種の同定
リー及び駒形（Ｌｅｅ ＆ ＫＯｍａｇａｔａ ） （
／９ＫＯ）のメタノール資化菌の分類学的研究
喋り
’ Ｔａｘｏｎｏｍｉｃａｌ ５ｔｕｄｙ ｏｆ
ｍｓｔｈａｎｏｌａｓｓｉｍｉｌａｔｉｎｇｙｅａｓ
ｔｓ ’によればカンデイダ属の中にメタノール資化菌
桟として３種を認めている。■ Species identification Lee & Komagata (Lee & Komagata)
/9KO)'s taxonomic research on methanol-assimilating bacteria' Taxonomical 5tudy of
msthanolassimilatingyeas
According to TS', three species of methanol-assimilating bacteria are recognized within the genus Candeida.
（Ｇ、ｂｏｌｉｎｌｌ 、 Ｇ、ｃａｒｉｏａｉｌｉｇ
ｎｉｃｏｌａ 。(G, bolinll, G, carioailig
nicola.
０．１１ｐｏｌｙｔｉｃａ 、 Ｏ，５ｕｃｃｉｐｈｉ
ｌａ 、 Ｏ，ｕｔｉｌｉｓ）。0.11polytica, O,5ucciphi
la, O. utilis).
これらの５種は糖類の発酵能パターン及び糖類の資化性
パターンによって主に識別されている。These five types are mainly distinguished by their saccharide fermentability patterns and saccharide assimilation patterns.
本菌株（Ａ−１９１７−３）の糖類の発酵性試験及び上
記の炭素源の資化性パターンはロダー（／デフ０）のザ
・イースト及びり−と駒形（／９１０）によって記載さ
れているカンデイダ・ボイデイニイ（Ｃａｎｄｉｄａ
ｂｏｉｃＬｉｎｉｉ ）の諸性質とよく合致する。The saccharide fermentability test of this strain (A-1917-3) and the carbon source assimilation pattern described above are described by The East and Riito Komagata (/910) of Roder (/def0). Candida Boideinii
boicLinii).
よって本菌株（Ａ−／？／７−Ｊ ）はカンデイダ拳ボ
イデイニイ（○ａｎｄｉｄａ ｂｄｉｄｉｎｉｉ ）と
同定された。また本菌株（Ａ−ｔ ｑ／ ７−、？ ）
とカンデイダ・ボイデイニイ（Ｃａｎｄｉｄａ ｂｏｌ
ｉ−ｎ１１〕のＡｕｔｈｅｎｔｉｃ ５ｔｒａｉｎ （
工ＡＭ ／ココ６デ株）の形態上の特徴、糖類の発酵能
、炭素源の資化性、及び他の生理的性質について比較検
討したところ１本菌株（Ａ−／９／７−Ｊ ）の諸性質
はカンデイダ・ボイデイニイ（工ＡＭ／２２６？）のそ
れらともよく一致した。Therefore, this strain (A-/?/7-J) was identified as Candida bdidinii. Also, this strain (A-t q/ 7-,?)
and Candida bol
i-n11] Authentic 5train (
A comparative study of the morphological characteristics, sugar fermentation ability, carbon source assimilation ability, and other physiological properties of the A-/9/7-J strain was conducted. The properties of Candida boideinii (Eng. AM/226?) agreed well.
本発明において使用される培地としては、主炭素源とし
てメタノールを含むものであれば、特に制限されない。The medium used in the present invention is not particularly limited as long as it contains methanol as a main carbon source.
炭素源としては、メタノール以外に、種々の炭水化物、
有機酸等をさらに添加してもよく、窒素源としては、有
機アンモニウム塩、無機アンモニウム塩、尿素等を用い
ることができる。In addition to methanol, various carbohydrates,
An organic acid or the like may be further added, and organic ammonium salts, inorganic ammonium salts, urea, etc. can be used as the nitrogen source.
また、必要に応じ、無機物として各種リン酸塩、硫酸塩
等を使用することができ、必要に応じ各種有機栄養物を
添加することもできる。Furthermore, various phosphates, sulfates, etc. can be used as inorganic substances, and various organic nutrients can also be added as necessary.
培養は、通常ｌコ時間〜ｌＩＩ日間程度、好気的条件下
に行なわれる。 、１１．。Cultivation is usually carried out under aerobic conditions for about 1 hour to 12 days. , 11. .
（実施例） 以下、実施例により、本発明をさらに説明する。(Example) The present invention will be further explained below with reference to Examples.
なお、実施例における物質の同定はガスクロマトグラフ
−質量分析等により標品と比較して行なった。In addition, the identification of the substances in the examples was carried out by comparison with standard products by gas chromatography-mass spectrometry and the like.
（実施例１）
メタノール１１０１．ＮＨ４０１１ダ＃ 、 ｘ、ａｐ
ｏ４／ 、９ 。(Example 1) Methanol 1101. NH4011 da #, x, ap
o4/, 9.
Claims (1)
オン酸を生産する能力を有する微生物を、炭素源として
メタノールを使用して培養し、培養物からプロピオン酸
を得ることを特徴とするプロピオン酸の製造方法。(1) A method for producing propionic acid, which comprises culturing a microorganism belonging to the genus Candida and having the ability to produce propionic acid using methanol as a carbon source, and obtaining propionic acid from the culture. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21424984A JPS6192583A (en) | 1984-10-15 | 1984-10-15 | Production of propionic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21424984A JPS6192583A (en) | 1984-10-15 | 1984-10-15 | Production of propionic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6192583A true JPS6192583A (en) | 1986-05-10 |
| JPH0424988B2 JPH0424988B2 (en) | 1992-04-28 |
Family
ID=16652636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21424984A Granted JPS6192583A (en) | 1984-10-15 | 1984-10-15 | Production of propionic acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6192583A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100917889B1 (en) | 2006-11-01 | 2009-09-16 | 삼성전자주식회사 | Apparatus and method for wireless communication |
-
1984
- 1984-10-15 JP JP21424984A patent/JPS6192583A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0424988B2 (en) | 1992-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |