JPS6238657B2 - - Google Patents

Info

Publication number: JPS6238657B2
Authority: JP; Japan
Prior art keywords: creatinine; bilirubin; rate; picrate; biological sample
Prior art date: 1978-08-09
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP9616978A

Other languages

English (en)

Japanese (ja)

Other versions

JPS5523445A (en

Inventor

Toshuki Sagusa

Yasushi Nomura

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Hitachi Ltd

Original Assignee

Hitachi Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-08-09

Filing date

1978-08-09

Publication date

1987-08-19

1978-08-09 Application filed by Hitachi Ltd filed Critical Hitachi Ltd

1978-08-09 Priority to JP9616978A priority Critical patent/JPS5523445A/ja

1980-02-19 Publication of JPS5523445A publication Critical patent/JPS5523445A/ja

1987-08-19 Publication of JPS6238657B2 publication Critical patent/JPS6238657B2/ja

Status Granted legal-status Critical Current

Links

DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 claims description 146
BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 claims description 82
229940109239 creatinine Drugs 0.000 claims description 73
238000000034 method Methods 0.000 claims description 42
238000006243 chemical reaction Methods 0.000 claims description 26
229940075930 picrate Drugs 0.000 claims description 18
OXNIZHLAWKMVMX-UHFFFAOYSA-M picrate anion Chemical compound [O-]C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-M 0.000 claims description 18
239000003153 chemical reaction reagent Substances 0.000 claims description 17
OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims description 14
239000012472 biological sample Substances 0.000 claims description 13
238000004519 manufacturing process Methods 0.000 claims description 12
QDHHCQZDFGDHMP-UHFFFAOYSA-N Chloramine Chemical compound ClN QDHHCQZDFGDHMP-UHFFFAOYSA-N 0.000 claims description 9
VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 claims description 4
CEJLBZWIKQJOAT-UHFFFAOYSA-N dichloroisocyanuric acid Chemical compound ClN1C(=O)NC(=O)N(Cl)C1=O CEJLBZWIKQJOAT-UHFFFAOYSA-N 0.000 claims description 4
239000005708 Sodium hypochlorite Substances 0.000 claims description 3
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
238000010521 absorption reaction Methods 0.000 description 17
238000005259 measurement Methods 0.000 description 14
210000002966 serum Anatomy 0.000 description 14
238000001228 spectrum Methods 0.000 description 12
239000000126 substance Substances 0.000 description 12
239000007800 oxidant agent Substances 0.000 description 11
230000003647 oxidation Effects 0.000 description 9
238000007254 oxidation reaction Methods 0.000 description 9
238000012360 testing method Methods 0.000 description 9
102000004169 proteins and genes Human genes 0.000 description 8
108090000623 proteins and genes Proteins 0.000 description 8
239000012085 test solution Substances 0.000 description 6
238000010876 biochemical test Methods 0.000 description 5
238000004737 colorimetric analysis Methods 0.000 description 5
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
102000004190 Enzymes Human genes 0.000 description 4
108090000790 Enzymes Proteins 0.000 description 4
238000002835 absorbance Methods 0.000 description 4
238000010586 diagram Methods 0.000 description 4
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
239000003638 chemical reducing agent Substances 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
230000000694 effects Effects 0.000 description 3
238000006911 enzymatic reaction Methods 0.000 description 3
230000002452 interceptive effect Effects 0.000 description 3
238000011533 pre-incubation Methods 0.000 description 3
239000003223 protective agent Substances 0.000 description 3
239000000523 sample Substances 0.000 description 3
239000000243 solution Substances 0.000 description 3
239000012086 standard solution Substances 0.000 description 3
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
GWZYPXHJIZCRAJ-UHFFFAOYSA-N Biliverdin Natural products CC1=C(C=C)C(=C/C2=NC(=Cc3[nH]c(C=C/4NC(=O)C(=C4C)C=C)c(C)c3CCC(=O)O)C(=C2C)CCC(=O)O)NC1=O GWZYPXHJIZCRAJ-UHFFFAOYSA-N 0.000 description 2
RCNSAJSGRJSBKK-NSQVQWHSSA-N Biliverdin IX Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(\C=C/2C(=C(C)C(=C/C=3C(=C(C=C)C(=O)N=3)C)/N\2)CCC(O)=O)N1 RCNSAJSGRJSBKK-NSQVQWHSSA-N 0.000 description 2
ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
206010023126 Jaundice Diseases 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
QBUVFDKTZJNUPP-UHFFFAOYSA-N biliverdin-IXalpha Natural products N1C(=O)C(C)=C(C=C)C1=CC1=C(C)C(CCC(O)=O)=C(C=C2C(=C(C)C(C=C3C(=C(C=C)C(=O)N3)C)=N2)CCC(O)=O)N1 QBUVFDKTZJNUPP-UHFFFAOYSA-N 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
239000000460 chlorine Substances 0.000 description 2
229910052801 chlorine Inorganic materials 0.000 description 2
230000007423 decrease Effects 0.000 description 2
238000007865 diluting Methods 0.000 description 2
238000011534 incubation Methods 0.000 description 2
238000000691 measurement method Methods 0.000 description 2
230000001590 oxidative effect Effects 0.000 description 2
230000001737 promoting effect Effects 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
235000000346 sugar Nutrition 0.000 description 2
150000008163 sugars Chemical class 0.000 description 2
239000004094 surface-active agent Substances 0.000 description 2
230000002123 temporal effect Effects 0.000 description 2
210000002700 urine Anatomy 0.000 description 2
102000035195 Peptidases Human genes 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
230000001133 acceleration Effects 0.000 description 1
239000002253 acid Substances 0.000 description 1
239000003513 alkali Substances 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
235000010323 ascorbic acid Nutrition 0.000 description 1
229960005070 ascorbic acid Drugs 0.000 description 1
239000011668 ascorbic acid Substances 0.000 description 1
229910021538 borax Inorganic materials 0.000 description 1
239000013043 chemical agent Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000011161 development Methods 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
229910000397 disodium phosphate Inorganic materials 0.000 description 1
235000019800 disodium phosphate Nutrition 0.000 description 1
238000009585 enzyme analysis Methods 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000001914 filtration Methods 0.000 description 1
ICYOLCFDSJJLAC-UHFFFAOYSA-N gramine Chemical compound C1=CC=C[C]2C(CN(C)C)=CN=C21 ICYOLCFDSJJLAC-UHFFFAOYSA-N 0.000 description 1
238000011835 investigation Methods 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
239000002207 metabolite Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
210000003205 muscle Anatomy 0.000 description 1
-1 permanganate Chemical compound 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
235000019833 protease Nutrition 0.000 description 1
230000008085 renal dysfunction Effects 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
235000019333 sodium laurylsulphate Nutrition 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
229940035637 spectrum-4 Drugs 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

JP9616978A 1978-08-09 1978-08-09 Measuring method for reaction rate of creatinine Granted JPS5523445A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP9616978A JPS5523445A (en)	1978-08-09	1978-08-09	Measuring method for reaction rate of creatinine

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP9616978A JPS5523445A (en)	1978-08-09	1978-08-09	Measuring method for reaction rate of creatinine

Publications (2)

Publication Number	Publication Date
JPS5523445A JPS5523445A (en)	1980-02-19
JPS6238657B2 true JPS6238657B2 (cs)	1987-08-19

Family

ID=14157822

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP9616978A Granted JPS5523445A (en)	1978-08-09	1978-08-09	Measuring method for reaction rate of creatinine

Country Status (1)

Country	Link
JP (1)	JPS5523445A (cs)

1978
- 1978-08-09 JP JP9616978A patent/JPS5523445A/ja active Granted

Also Published As

Publication number	Publication date
JPS5523445A (en)	1980-02-19

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