JPS6283662A - Method for extracting component by thin layer chromatography - Google Patents
Method for extracting component by thin layer chromatographyInfo
- Publication number
- JPS6283662A JPS6283662A JP22346985A JP22346985A JPS6283662A JP S6283662 A JPS6283662 A JP S6283662A JP 22346985 A JP22346985 A JP 22346985A JP 22346985 A JP22346985 A JP 22346985A JP S6283662 A JPS6283662 A JP S6283662A
- Authority
- JP
- Japan
- Prior art keywords
- plate
- thin layer
- separated component
- kbr
- apex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
【発明の詳細な説明】
〔発明の技術分野〕
この発明は、有機系化合物の混合物の抽出方法、％に７
！ ＋ｔ１クロマトグラフィーによって分離さ７１．る
成分の抽出方法に関する。[Detailed Description of the Invention] [Technical Field of the Invention] This invention relates to a method for extracting a mixture of organic compounds,
! +t1 chromatography separated71. The present invention relates to a method for extracting components.
従来、薄層クロマトグラフィーに↓り薄層板上で分離さ
れた成分を分取する場合、その分離成分ごと薄層担体を
ガラス板から剥離して、ざらに、ビーカー等ＶＣ＃シ、
有機溶媒を注入して２〜３分間超音波洗浄を行ない、薄
層担体から分離成分を抽出したのち、濾過して分取成分
のみを取り出し、有機溶媒を揮散させて濃縮するという
煩雑な操作が必要であった。従って、使用する溶媒着が
多くなるため、その中に含まれる微量不純物量も多くな
るという問題があった。まだ、濾過を確実Ｖこ行なわな
いと分取物の方に′４層担体粉が入り込むため、この点
でも注意が８渋であった。Conventionally, when separating components on a thin layer plate using thin layer chromatography, the thin layer carrier was peeled off from the glass plate along with the separated components, and placed in a VC# container such as a beaker.
The complicated operation of injecting an organic solvent, performing ultrasonic cleaning for 2 to 3 minutes, extracting the separated components from the thin layer carrier, filtering to extract only the fractionated components, and evaporating the organic solvent and concentrating is performed. It was necessary. Therefore, since a large amount of solvent is used, there is a problem that the amount of trace impurities contained therein also increases. If the filtration is not carried out properly, the 4-layer carrier powder will enter the fraction, so care must be taken in this respect as well.
本発明は、上述した従来法の問題点に鑑みなされたもの
であり、その目的とするものは薄層板の薄層担体を剥離
するという煩雑な操作を行なうことなく、しかも少量の
溶媒でその中Ｖこ含有される微せ不純物の影響にｔ＃に
注意を払うことなく薄層板上の各分離成分を分取できる
方法を提供することにある。The present invention was made in view of the problems of the conventional method described above, and its purpose is to do so without the complicated operation of peeling off the thin layer carrier of the thin layer plate, and moreover, with a small amount of solvent. The object of the present invention is to provide a method by which each separated component on a thin layer plate can be fractionated without paying attention to the influence of slight impurities contained in the t#.
本発明は、従来法のようＶＣ分離成分を薄層担体ごと剥
離してから溶媒を用いて分取するという煩雑な方法で７
１なく、溶媒を用いて目的とする分離成分を薄層板上で
移動させ、薄層、板上の一端に固定した三角形状、好１
しくけ二等辺三角形状の臭化カリウム板の頂点付近に毛
細管現象を利用して１縮するものである。友だし、薄層
板上で有機系化合物の混合試料を分離する操作は従来通
り試料を帯状ＶＣ薄層板の一端ＶＣ塗布し、適当な溶媒
を用いて展開することによって行なうが、展開後薄層板
上で分離した成分をそれぞれ分取する操作はこの臭化カ
リウム板を利用して濃縮するわけである。The present invention eliminates the complicated method of peeling off the VC separated component along with the thin layer carrier and then separating it using a solvent, as in the conventional method.
1, the target separated component is moved on a thin layer plate using a solvent, and the thin layer is triangular shaped and fixed at one end of the plate, preferably 1.
A potassium bromide plate in the form of an isosceles triangular structure is contracted by 1 near the apex using capillary phenomenon. The operation of separating a mixed sample of organic compounds on a thin laminate is conventionally carried out by coating the sample with VC on one end of the strip-shaped VC thin laminate and developing it using an appropriate solvent. The potassium bromide plate is used to concentrate the components separated on the layer plate.
臭化カリウム板は、通常治具を用いて薄層仮に固定され
るが、第一に臭化カリウム板が固定された位置からｆ
ｆ’Ｌ ｌいこと、第二に薄層担体と密着して隙間があ
かないこと、第三Ｖ′ｃ薄層板と臭化カリウム板とのな
す角度を９０変だけでなく任意にとれること、などの条
件をｉ４Ａだせばどのようｆｒ、固定具であってもか壕
わない。The potassium bromide plate is usually temporarily fixed in a thin layer using a jig, but first the f
f'L is small; second, it is in close contact with the thin layer carrier with no gaps; third, the angle between the V'c thin layer plate and the potassium bromide plate can be set not only at 90 degrees but also at any angle. If the conditions such as , etc. are set as i4A, no matter how fr or fixings are used, it will not be affected.
本発明によれば、従来法のように薄層板上で分離された
成分を分取する際、煩雑な操作を必要としないのでこの
ときに混入する不純物がなくなり、しかも操作時間を大
幅に短縮できる。さらに、従来法のように多畦の抽出溶
媒を必要としないので、この溶媒中に溶存している微量
の不純物が濃縮され、目的Ｑ分取物中に混入するか否か
に注意を払う必要がない。また、操作時間が短縮される
ばかりでなく、臭化カリウム板を装着してから濃縮が完
了するまでの間は濃縮状態を観察する程度でよいため煩
わしさがない。According to the present invention, there is no need for complicated operations when fractionating the components separated on a thin layer plate as in the conventional method, so there are no impurities mixed in at this time, and the operation time is significantly shortened. can. Furthermore, unlike conventional methods, multiple extraction solvents are not required, so it is necessary to pay attention to whether trace amounts of impurities dissolved in this solvent will be concentrated and mixed into the target Q fraction. There is no. Moreover, not only is the operation time shortened, but it is also unnecessary to observe the concentration state from the time the potassium bromide plate is attached until the concentration is completed, so there is no inconvenience.
以下５図面に基づいて本発明の実施例について説明する
。本発明を実施する場合に必要となる装置は、シリカゲ
ル、アルミナ、セルロースなト数〜士数ミクロンの均一
な粒径を有する粒子を一定厚さで、無機系ゝ不溶性螢光
剤とともにガラス板に塗布した薄層板１、その薄層板１
上に璋布した有機化合物の混合物を溶媒を用いて展開（
分＠）するためのガラス製の容器２、薄層板１上で分離
された各成分をそれぞれ濃縮するだめの二等辺三角形（
８ｘ２４ｘ３ｍｍ）の臭化カリウム板３、薄層板１上Ｖ
こ任意の角変で臭化カリウム板３を固定するための固定
具４が必要である。試料となる有機化合物の混合物を第
１図の（ａ）の位置に帯状に塗布し、展開容器２の中で
溶媒を用いて展開する。試料は第２図のようにｉｓ層板
１上で分離する。臭化カリウム板３は、第３図に示した
ように固定具４を用いて固定し、第１図の右で示したよ
うに展開槽２の中で濃縮用の溶媒５を用いて各分離成分
を濃縮する。この際、溶媒５が毛細管現象ｖｃよって薄
ｆ−板ｌを次第に上昇し、臭化カリウム板３の頂点まで
各分離成分を押し上げて濃縮させる。頂点まで濃縮され
た各分離成分は、頂点を切り取ることで分取され、さら
に臭化カリウム錠剤９を作成して赤外分光分析用試料と
して供するとともに定性を行なう。なお、、；Ｂ４図は
従来法と本発明との比較をしたものである。Embodiments of the present invention will be described below based on five drawings. The equipment required to carry out the present invention is to deposit particles of silica gel, alumina, cellulose, etc. having a uniform particle size of a few microns to a few microns on a glass plate together with an inorganic insoluble fluorescent agent. Coated laminate 1, laminate 1
The mixture of organic compounds spread on top is developed using a solvent (
glass container 2 for concentrating each component separated on the thin layer plate 1, and an isosceles triangle (
8x24x3mm) potassium bromide plate 3, thin layer plate 1 V
A fixture 4 is required to secure the potassium bromide plate 3 at any angle. A mixture of organic compounds to be a sample is applied in a strip at the position (a) in FIG. 1, and developed in a developing container 2 using a solvent. The sample is separated on the IS layer plate 1 as shown in FIG. The potassium bromide plate 3 is fixed using a fixture 4 as shown in FIG. Concentrate ingredients. At this time, the solvent 5 gradually rises up the thin F-plate 1 due to capillary action VC, pushing up each separated component to the top of the potassium bromide plate 3 and concentrating it. Each separated component concentrated to the top is separated by cutting off the top, and a potassium bromide tablet 9 is further prepared and used as a sample for infrared spectroscopic analysis, as well as being qualitatively analyzed. Note that Figure B4 shows a comparison between the conventional method and the present invention.
第１図は本発明方法の実施例，態を説明するための薄層
クロマトグラフィーの概略図、第２図は展開した薄層板
の図、第３図は臭化カリウム板の固定法の一例を示した
図、第４図は従来法と本発明方法の比較図である。
ｌ・・・薄層クロマトグラフィー用薄層板２・・・展開
槽 ３・・臭化カリウム版４・・臭化カリウム板の固
定具
５・・・濃縮用溶媒 ６・・・抽出用溶媒７・・・戸
紙
代理人 弁理士 則 近 憲 佑
同 竹 花 喜久男
手 続 補 正 ・ト方式 ）昭和６「計２．
亡４ 日
％許庁長宮殿
１、事件の表示
特願昭６０−２２３４６９号
２ 発明の名称
薄層クロマトグラフィーによる成分抽出方法３、補正を
する者
！μ件との関係 特許出願人
（３０７）株式会社 東芝
１、代理人
〒１０５
東京都港区芝浦−丁目１番１号
昭和６１年１月２８日（発送日）
６、 補正の対象
願書に最初に添付した明細書
７、 補正の内容Figure 1 is a schematic diagram of thin layer chromatography for explaining an embodiment of the method of the present invention, Figure 2 is a diagram of an expanded thin layer plate, and Figure 3 is an example of a method for fixing a potassium bromide plate. FIG. 4 is a comparison diagram of the conventional method and the method of the present invention. l... Thin layer plate for thin layer chromatography 2... Development tank 3... Potassium bromide plate 4... Potassium bromide plate fixture 5... Solvent for concentration 6... Solvent for extraction 7 ...Togami Agent Patent Attorney Rule Chika Ken Yudo Takehana Kikuo Procedure Amendment ・To method) Showa 6 ``Total 2.
4th day of his death 1, Indication of the incident Patent Application No. 1988-223469 2 Name of the invention Method for extracting components by thin layer chromatography 3, person making corrections! Relationship with Matter μ Patent Applicant (307) Toshiba Corporation 1, Agent Address: 1-1 Shibaura-chome, Minato-ku, Tokyo 105 January 28, 1986 (Date of dispatch) 6. First application to be amended Description 7 attached to , Contents of amendment
Claims (1)
厚さで、無機系不溶性螢光剤とともにガラス板に塗布し
た薄層板の一端に、試料となる有機系化合物の混合物を
塗布し、有機溶媒を用いて展開したのち、分離された各
成分を薄層板から分取する成分抽出において、三角形状
の臭化カリウム板を薄層板に固定し、臭化カリウム板の
頂点付近に各分離成分を濃縮することからなることを特
徴とする薄層クロマトグラフィーによる成分抽出方法。A mixture of organic compounds to be used as a sample is applied to one end of a thin glass plate in which a filler with a uniform particle size of several to several tens of microns is applied to a constant thickness along with an inorganic insoluble fluorescent agent. In component extraction, in which each separated component is extracted from a thin layer plate after development using an organic solvent, a triangular potassium bromide plate is fixed to the thin layer plate, and a triangular potassium bromide plate is placed near the apex of the potassium bromide plate. A method for extracting components by thin layer chromatography, characterized by concentrating each separated component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22346985A JPS6283662A (en) | 1985-10-09 | 1985-10-09 | Method for extracting component by thin layer chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22346985A JPS6283662A (en) | 1985-10-09 | 1985-10-09 | Method for extracting component by thin layer chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6283662A true JPS6283662A (en) | 1987-04-17 |
Family
ID=16798625
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22346985A Pending JPS6283662A (en) | 1985-10-09 | 1985-10-09 | Method for extracting component by thin layer chromatography |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6283662A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0283465U (en) * | 1988-12-14 | 1990-06-28 | ||
| JPH0283466U (en) * | 1988-12-14 | 1990-06-28 | ||
| JPH0283467U (en) * | 1988-12-14 | 1990-06-28 |
-
1985
- 1985-10-09 JP JP22346985A patent/JPS6283662A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0283465U (en) * | 1988-12-14 | 1990-06-28 | ||
| JPH0283466U (en) * | 1988-12-14 | 1990-06-28 | ||
| JPH0283467U (en) * | 1988-12-14 | 1990-06-28 |
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