JPS63287449A - Pet food containing enzymatic hydrolyzate of blood cells - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed Description of the Invention] (Field of Application of Table 1) The present invention is a one-layer curved blood cell! The fermented hydrolyzate mentioned when #1E is applied, or a mixture of two plasmas, is added to pet food and its purpose is #1. The aim is to reduce the amount of xirk excreted by pets by making effective use of bent blood, and by adding it to pet food or partially substituting it.

(Prior Art) Most of the solid content of livestock blood flowing out from slaughterhouses is composed of proteins. In addition, the proteins contained in this blood vessel are related to plasma, which is mostly composed of simple proteins, using centrifugation. Blood cells can be easily separated into two fractions.

Plasma fraction in blood and truck fluid, f') Simple proteins include albumin, globulin, fifirinogen 7, etc., and although they are widely used in IR as *products or medical products, The use of hemoglobin, a protein-negative hemoglobin, has not yet been established and is mostly discarded in the form of lumps.

On the other hand, the food given to dogs and anchors is more or less the same as that of humans.
I was given what was left over from Mr. J.
In recent years, there has been a pet boom and rivet food! Praise is MatyoMacho = t. In addition, the types of pet food are diversifying, and the quality, including palatability and honor value, has significantly improved.

However, due to the increase in the number of pets, the capital is increasing.

In Nunobe, dog and cat defecation has become a kind of pollution hazard, and many cities have enacted ordinances, with pet owners facing negative penalties. It is face down.

Despite the fact that the hemocyte cell hydrolyzate obtained by the warp treatment (which the present invention is trying to solve) is monostoichiometric.

This fluorescein has a low molecular weight, and also contains calcium, fs, and other minerals. In addition, since it does not have the odor of vm, by adding nitrate to pet food or partially substituting it for pet food, it is possible to use 1jII blood in a self-effective manner and reduce the excretion of pets. We are manufacturing a pet product that can reduce the number of fireflies. Furthermore, the present invention is related to the external push of a truck by a m-* trader. It is something that will be resolved.

(Means for solving the problem) The present inventors have repeatedly investigated methods for using pet food as an effective method of utilizing blood cells obtained from N. Aisara Toru. It is a known fact that a white powder product can be obtained as an enzymatic hydrolyzate by treating blood cells with protease Y and drying. (NEW FOODINI) USTRY Vol.
, 26 No. 8 1984) To explain the present invention in more detail, blood cells obtained from #a-hospital blood have a plate curve σJ
There are no particular restrictions on the type of glaze; generally, cow or glaze blood is used.

This collected m liquid! Use the centrifugal fraction *aey to obtain the sedimentation fraction, that is, the plate, and also centrifuge fraction l@! according to your needs! Repeated cutting can increase the yield of blood reeds. The simplest and best method for obtaining blood cells V is to use a centrifuge, but the method is not limited to this method.Add 2 to 5 times the amount of water to the obtained blood cells! In addition, adjust the protein level of the blood cell bath solution@After that, use alkaline Y to adjust the standard pH'
Jk-7~IO prefert L < It P ) i yt
8.2~g, 7t=v14 tatami. Is this yeast AC? Add 0.1-10% for negative blood cell protein, bath temperature 40-70
Hydrolysis is carried out for 7 times at ℃, followed by centrifugation and purification using activated carbon or ion exchange resin. By the way, is there water in the residue after centrifugation? The yield of enzymatic hydrolyzate can be increased by repeating the addition of centrifugation.

Leave the enzymatic hydrolyzate as an autostatic solution after making it.

Alternatively, it is possible to mix it with plasma and use it on a vinegar solution, but from the viewpoint of changes in the obtained protein over time, storage, and transportation, it is possible to use frozen late pulse, vacuum @, travel, and spray drying. It is more preferable to use it in the form of a powder.As for the method of hydrolyzing blood cells, the most preferable method is to use an enzyme; there are two limitations. , or by mixing with blood plasma, it can be mixed with pet 7-doke during production. Addition of dog2 can be expected to be more effective. The foil loading is particularly (but not limited to) 0.1 to the vet food as an enzymatic hydrolyzate or by mixing with plasma.
It is appropriate to add up to 10%, but there is no problem with adding more than this amount.

The pet food to which the vinegar hydrolyzate is added may be dry type, soft dry type, semi-moist type, wet type, milk, retort, jerky, etc., and the shape may be extract band type, It can be added to any type of vet food such as pellet type, granules, cubes, freeze-dried tablets, etc. The fluorescent YT4 composition of this enzymatic hydrolyzate is composed of several glazes of amino acids bonded to peptides, making it a dispersion. The molecular weight distribution of the hydrolyzate determined by chromatography is mostly concentrated in the range of 000 to 2000, but it is widely
Distributed from 00 to 5000.

There is also no strange odor that is typical of blood donation.

Therefore, this pancreatic cord hydrolyzed compound is mixed at the time of manufacturing Kepet 7-d, or it is added to commercially available pet food and given to dogs and cats, and its palatability is observed and excreted g ii O) is measured. As a result, it was reported that even with the addition of this #cumulative hydrolyzate!, there was no change in palatability, and the amount of leaves excreted was reduced.

Examples of the present invention will be shown below.
It is not limited to two.

Example 1゜After adding 25% sodium citrate water bath solution to 1 liter collected from #1 Kumofun, it is centrifuged and separated into plasma and blood cells. Add 5 liters of water,
The PH''Ik was adjusted to 8.5 using the specified Na/OR solution, and 3% protease, which is a hydrolysis enzyme of insect whites, was added to the blood cell bath solution, and the mixture was kept at a temperature of 55°C for about 3 hours.

Next, PHY5. After adjusting the temperature, add activated carbon to the overlying solution (Soiko Yokusou) at 5% to the total bath solution, pass through the suction port for Toyo pJaNo. By drying, 1.1 kg of a white powdery dry product 'Ik- was obtained.

The amount of protein in this dried product was measured by the Kelpool method and was found to contain approximately 85%.

The 'rS fermentation cumulative hydrolyzate obtained in this manner was added to a small amount of commercially available Betfood Don: &J dissolved in warm water, mixed well, and administered to a 6-month-old Shiba Inu at 200f'% per day.
'I gave 1 toruma in 2 times C in 2 parts, and then on 3rd (b), I gave 1R of Tsueiri to both of them.

Added to commercially available PET 7-do at a rate of 1%, 3%, and 5%.
Monoke, each as a test area.

It is set as a commercially available pet food.

Tests were carried out.Subjected to the test: Three Shiba Inu dogs were used in each test section and control section. The results are shown in Table 1.

As is clear from Table 1, Section V, the bulk of Eidai could be reduced by using the # cumulative hydrolyzate in a commercially available pet food after cooling it.

Also, in terms of palatability, there is no difference from commercially available pet food.

Example 2 #Cumulative hydrolysis counterfeit obtained in the same manner as in Example 1 was added to commercially available pet food at a ratio of 1%, 3%, and 5% (σ). , Cats fed only commercially available pet food. 111 set as a control area
After feeding 100 fl' twice a day for one week, measurements were taken by punching cat feces fV31F in each area.

The cats used in the test were cats that were one year old.Three cats were used in each test group and control group. The results are shown in Table 2.

As is clear from the @2 table, enzymatic hydrolysis w1'4r can reduce the bulk of cats by adding it to commercially available pet food.

In terms of bite, there was no difference from commercially available pet food.

Example 3 Approximately 6 tons of plasma obtained by centrifuging 101 forest product Y35000r, p, m for 15 minutes was freeze-dried to obtain a white powder product of approximately 4502. Mix it with the #cumulative hydrolyzate obtained by C2 in the same manner as in 1 at a ratio of 1:l, and add it to Tfi pet food at a ratio of 1%, 3%, and 5% to make L! , 6-month-old Eidai was fed 200v per day divided into 2 doses for 1 week, and then the prize violet was measured 3 times. The Shiba Inu dogs that were fed were set up, and the Shiba Inu dogs that were subjected to the sleeping test sang three songs each in each test group and the control group.The test results are shown in Table V.

Table 3

Claims (2)

[Claims] (1) A pet food characterized by adding a hydrolyzate obtained by enzymatically treating blood cells in slaughtered blood. (2) A pet food characterized by mixing plasma with a hydrolyzate obtained by enzymatically treating blood cells in slaughtered blood, and adding the mixture.