JPS6410175A - Analysis element - Google Patents

Abstract

PURPOSE:To improve detection sensitivity and reproducibility by providing a timing layer consisting of a water soluble binder or gel between two porous reaction layers 2 and 4. CONSTITUTION:This analysis element consists of a transparent base 1 consisting of glass, etc., the porous reaction layer 2 fixed with a material B which modulates the labeling signal of a labeling material W by conjugating specifically with said material, the timing layer 3, and the porous reaction layer 4 fixed with either of a biologically active material A1 which does not conjugate with the specific component X in a fluid sample or a material A2 which can conjugate specifically to the material A1. A material Y which is conjugated with the material A1 or A2 unfixed to the layer 4 and reacts specifically with the specific component X and a labeled body Z formed by conjugating the labeling material W to the specific component X are added to a prescribed amt. of the sample and the sample is dropped onto the analysis element. The layer 3 dissolves and the unconjugated labeled body Z conjugates with the layer 2 after the sufficient progression of the competitive reaction of the materials X, 4 and the material Y and the conjugation reaction of the material Y and the layer 4 and, therefore, the detection sensitivity and reproducibility of the concn. of the component X are improved to a greater extent.