JPS641962A - Solid phase antibody for immunological analysis - Google Patents
Solid phase antibody for immunological analysisInfo
- Publication number
- JPS641962A JPS641962A JP15559487A JP15559487A JPS641962A JP S641962 A JPS641962 A JP S641962A JP 15559487 A JP15559487 A JP 15559487A JP 15559487 A JP15559487 A JP 15559487A JP S641962 A JPS641962 A JP S641962A
- Authority
- JP
- Japan
- Prior art keywords
- solid phase
- antibody
- liquid
- immunological analysis
- bio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PURPOSE: To expand the detection limit of an antigen in an immunological analysis on the side of a low density, by using a bioglass ball as solid phase for holding an antibody.
CONSTITUTION: A solid phase antibody for immunological analysis has an antibody adsorbed on the surface of a solid phase. A bioglass ball (BIO.B) is used as solid, an anti AFP-IgG as solid phase antibody and 125I-AFP as labelled antibody (AFT is abrr. for α-fetoprotein). The solid phase antibody covered with the anti AFP-IgG herein used is produced as follows: 6.5mmϕ of the BIO.B is immersed into a [liquid B] {0.1mg/mlIgG.0.1mol./l (pH 7.5) phosphoric acid buffer} of 4°C for one night, the BIO.B is washed three times by a liquid and again three times by [liquid C] {0.01mol/lNaCl, 0.1% BSA and 0.1% NaN3 mixed liquid} and, then, immersed into the liquid C of 4°C to preserve.
COPYRIGHT: (C)1989,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62-155594A JPH011962A (en) | 1987-06-24 | Solid phase antibody for immunoanalysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62-155594A JPH011962A (en) | 1987-06-24 | Solid phase antibody for immunoanalysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS641962A true JPS641962A (en) | 1989-01-06 |
| JPH011962A JPH011962A (en) | 1989-01-06 |
Family
ID=
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5921687A (en) * | 1991-05-24 | 1999-07-13 | Mitsubishi Denki Kabushiki Kaisha | Printing apparatus |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5921687A (en) * | 1991-05-24 | 1999-07-13 | Mitsubishi Denki Kabushiki Kaisha | Printing apparatus |
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