JPS6443138A - Method for regenerating plant body from protoplast of arboreous plant - Google Patents
Method for regenerating plant body from protoplast of arboreous plantInfo
- Publication number
- JPS6443138A JPS6443138A JP62199147A JP19914787A JPS6443138A JP S6443138 A JPS6443138 A JP S6443138A JP 62199147 A JP62199147 A JP 62199147A JP 19914787 A JP19914787 A JP 19914787A JP S6443138 A JPS6443138 A JP S6443138A
- Authority
- JP
- Japan
- Prior art keywords
- arboreous
- plants
- protoplasts
- plant
- calluses
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE:To readily obtain plant bodies of arboreous plants, by cultivating protoplasts of the arboreous plants together with protoplasts of herbaceous plants and differentiating the cultivated protoplasts. CONSTITUTION:Calluses or shoots or arboreous and herbaceous plants are preferably independently subjected to enzymic treatment to isolate protoplasts and both are mixed (preferably at 1:3-3:1 ratio of the arboreous plants to the herbaceous plants). The resultant mixture is then cultivated in an artificial liquid culture medium containing a composition of inorganic salts, support agent and plant hormones at 20-30 deg.C to form calluses. Only the calluses of the arboreous plants are transferred to a redifferentiation culture medium to regenerate shoots, roots and regrow the plant bodies.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62199147A JPS6443138A (en) | 1987-08-11 | 1987-08-11 | Method for regenerating plant body from protoplast of arboreous plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62199147A JPS6443138A (en) | 1987-08-11 | 1987-08-11 | Method for regenerating plant body from protoplast of arboreous plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6443138A true JPS6443138A (en) | 1989-02-15 |
| JPH0430810B2 JPH0430810B2 (en) | 1992-05-22 |
Family
ID=16402930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62199147A Granted JPS6443138A (en) | 1987-08-11 | 1987-08-11 | Method for regenerating plant body from protoplast of arboreous plant |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6443138A (en) |
-
1987
- 1987-08-11 JP JP62199147A patent/JPS6443138A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0430810B2 (en) | 1992-05-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |