JPS6480286A - Method for purifying enzyme - Google Patents
Method for purifying enzymeInfo
- Publication number
- JPS6480286A JPS6480286A JP23635087A JP23635087A JPS6480286A JP S6480286 A JPS6480286 A JP S6480286A JP 23635087 A JP23635087 A JP 23635087A JP 23635087 A JP23635087 A JP 23635087A JP S6480286 A JPS6480286 A JP S6480286A
- Authority
- JP
- Japan
- Prior art keywords
- lipase
- sephadex
- column chromatography
- gel
- cylindraceae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 102000004882 Lipase Human genes 0.000 abstract 6
- 108090001060 Lipase Proteins 0.000 abstract 6
- 239000004367 Lipase Substances 0.000 abstract 6
- 235000019421 lipase Nutrition 0.000 abstract 6
- 229920005654 Sephadex Polymers 0.000 abstract 3
- 239000012507 Sephadex™ Substances 0.000 abstract 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 3
- 238000004440 column chromatography Methods 0.000 abstract 3
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 abstract 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 abstract 2
- 102000005398 Monoacylglycerol Lipase Human genes 0.000 abstract 1
- 108020002334 Monoacylglycerol lipase Proteins 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 abstract 1
- 229910000397 disodium phosphate Inorganic materials 0.000 abstract 1
- 235000019800 disodium phosphate Nutrition 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 238000002523 gelfiltration Methods 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- 239000001488 sodium phosphate Substances 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE:To carry out fractionation into 1,3-diglyceride lipase and 2- monoglyceride lipase, by subjecting a lipase obtained by cultivating Candida.cylindraceae to treatment using column chromatography. CONSTITUTION:An ion exchange gel, such as DEAE-Sephadex(R) or SP- Sephadex(R), or gel for gel filtration, such as Sephadex(R) G-150 or G-100, or both are used as a carrier, treated with MacIlvaine buffer solution (0.01mol. citric acid and 0.02mol. disodium phosphate; pH 3.8), etc., filled in a column having 1-10cm diameter, 50-200cm length, etc., and used for column chromatography. A lipase obtained by cultivating Candida.cylindraceae, such as lipase OF, is treated by the above-mentioned column chromatography and fractionated into 1,3-diglyceride lipase and 2-monoglyceride lipase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23635087A JPS6480286A (en) | 1987-09-22 | 1987-09-22 | Method for purifying enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23635087A JPS6480286A (en) | 1987-09-22 | 1987-09-22 | Method for purifying enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6480286A true JPS6480286A (en) | 1989-03-27 |
Family
ID=16999505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP23635087A Pending JPS6480286A (en) | 1987-09-22 | 1987-09-22 | Method for purifying enzyme |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6480286A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100592871B1 (en) * | 2004-12-23 | 2006-06-26 | 재단법인 포항산업과학연구원 | Position fixing device of steel sheet to be custom welded |
| JP2016037556A (en) * | 2014-08-07 | 2016-03-22 | 横浜ゴム株式会社 | Rubber composition and pneumatic tire |
-
1987
- 1987-09-22 JP JP23635087A patent/JPS6480286A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100592871B1 (en) * | 2004-12-23 | 2006-06-26 | 재단법인 포항산업과학연구원 | Position fixing device of steel sheet to be custom welded |
| JP2016037556A (en) * | 2014-08-07 | 2016-03-22 | 横浜ゴム株式会社 | Rubber composition and pneumatic tire |
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