KR101242573B1 - Preparing method of Gel for treatment an ulcer including a honey and Gel for treatment an ulcer thereby - Google Patents

Abstract

The present invention relates to a method for preparing a gel for treating ulcers containing honey and a gel for treating ulcers prepared according to the present invention. Specifically, a biocompatible polymer, honey and a preservative are dissolved in purified water to prepare an aqueous solution (step 1). ; Pouring the aqueous solution of step 1 into a tray to form a gel sheet (step 2); And it provides a method for producing a gel for treating ulcers comprising honey, characterized in that it comprises a step (step 3) of irradiating the gel state sheet of step 2.
The gel prepared through the above method has the basic characteristics for use in wound treatment, water evaporation reduction, prevention of sensitization from body fluid-absorbing bacteria, adhesion to wounds or skin, transparency, ease of handling, storage and sterilization. It has an effect that can be applied as an antibiotic.

Description

Preparation method of gel for treating ulcers comprising honey and gels for treating ulcers prepared according to the present invention

The present invention relates to a method for preparing a gel for treating ulcers comprising honey and a gel for treating ulcers prepared accordingly.

An ulcer means that the epithelium has fallen out due to inflammation or necrosis, and the surface of the tissue is locally missing or depressed.When the inflammation progresses, tissue is destroyed by lysosomal enzymes from neutrophils and macrophages. Ulcers can occur when there is a problem with the arteries or veins that supply oxygen and nutrients, causing a sudden or slow blood supply. That is, all the causes that can cause inflammation is the cause of the ulcers, and inflammation by the bacterial infection proceeds and the formation of abscesses, part of the normal tissue is destroyed, and further develops ulcers.

Due to this development, ulcers can occur in the skin, as well as in the digestive, respiratory, cardiovascular, and genitourinary organs. In particular, the ulcers due to the lack of oxygen and nutrients in the tissue are typical of diabetic ulcers that occur mainly on the feet or legs, a complication due to diabetes.

Honey has been used as a folk remedy for trauma and stomatitis in the past, but honey has recently been reexamined as an antibiotic. In particular, as the concern about antibiotic resistance increases, research on the bactericidal ability of honey is being conducted.

Honey's ability to control bacteria has been transmitted since BC, and it has been called INHIBINE as an inhibitor of the unknown bacteria that honey has. In recent years, the scientific effects of honey on surgical wounds have been reported by Bromfield (1973) and Keast-Butler (1980) to be effective as a treatment for ulcers, particularly as a treatment for intractable ulcers. (Effem, 1988: McInerly, 1990; Zulma, 1989).

Willix et al. (1992) demonstrated the ability of honey to inhibit seven types of bacteria that are primarily involved in trauma. El-sukhon et al. (1994) found that honey's ability to inhibit bacteria was more effective in negative than in Gram-positive. It was reported that Bacillus cereus inhibits spore germination.

The bacteriostatic ability is related to the osmotic pressure caused by the hypertonic solution according to the high sugar content of honey and the low pH of the honey, but it is estimated to be mainly due to the hydrogen peroxide content (Bogodanov, 1984,1997; Allen et al., 1991; Mollan, 1992)

Therefore, the present inventors use the bactericidal ability of honey, which has been used as a folk remedy, to reduce the amount of evaporation of water than conventional therapeutics, to create an optimal wet environment for a long time, and to improve the adhesion and flexibility by the addition of honey. The gel for treating ulcers was completed.

It is an object of the present invention to provide a method of preparing a gel for treating ulcers comprising honey and a gel for treating ulcers prepared accordingly.

In order to achieve the above object, the present invention comprises the steps of preparing an aqueous solution by dissolving the biocompatible polymer, honey and preservative in purified water (step 1); Pouring the aqueous solution of step 1 into a tray to form a gel sheet (step 2); And it provides a method for producing a gel for treating ulcers comprising honey, characterized in that it comprises a step (step 3) of irradiating the gel state sheet of step 2.

The ulcer treatment gel prepared by the method for preparing the ulcer treatment gel containing honey of the present invention has basic properties for use in wound healing, water evaporation reduction, prevention of sensitization from body fluid absorption bacteria, adhesion to wounds or skin It has the properties of transparency, transparency, ease of handling, storage and sterilization, and can be applied as an antibiotic.

1 is a graph showing the gelation rate of the gel prepared by Examples 1 to 3 and Comparative Example 1 of the present invention;
2 is a graph showing the degree of water evaporation of the gel prepared by Examples 1 to 3 and Comparative Example 1 of the present invention;
3 is a graph showing the compressive strength of the gel prepared by Examples 1 to 3 and Comparative Example 1 of the present invention;
Figure 4 is a graph showing the results of animal experiments on the ulcer treatment effect of the gel prepared by Examples and Comparative Examples of the present invention.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The present invention comprises the steps of dissolving the biocompatible polymer, honey and preservatives in purified water to prepare an aqueous solution (step 1); Pouring the aqueous solution of step 1 into a tray to form a gel sheet (step 2); And it provides a method for producing a gel for treating ulcers comprising honey, characterized in that it comprises a step (step 3) of irradiating the gel state sheet of step 2.

Hereinafter, the present invention will be described in detail step by step.

Step 1 of the preparation method is a step of preparing an aqueous solution by dissolving the biocompatible polymer, honey and preservatives in purified water.

At this time, the biocompatible polymer used in step 1 is a synthetic polymer selected from the group consisting of polyvinyl alcohol, polyvinylpyrrolidone, polyacrylic acid and polyethylene oxide or carrageenan, sodium carboxymethyl cellulose, gelatin, agar, alginate and chitosan It is preferable that it is a natural polymer selected from the group which consists of, and it is more preferable that it is sodium carboxymethyl cellulose. The sodium carboxymethyl cellulose has the advantage of being suitable for use in the gel of the present invention because it has the property to contain water by including a hydrophilic functional group as a three-dimensional network structure.

In addition, the weight ratio of the biocompatible polymer mixed in the aqueous solution in step 1 is preferably 1 to 50% by weight.

If the biocompatible polymer to be mixed is less than 1% by weight, there is a problem in that it cannot maintain the gel strength enough to accommodate the drug to treat the wound, and when it exceeds 50% by weight, an aqueous solution is prepared. There is a problem of difficulty.

The weight ratio of the honey is mixed in the aqueous solution in step 1 is preferably 1 to 20% by weight.

If the weight ratio of the honey is less than 1% by weight can not sufficiently improve the adhesion and flexibility, if it exceeds 20% by weight can not maintain the gel strength enough to accommodate the drug.

In addition, the preservative added in step 1 is used to prevent the deterioration of the gel, and as an antioxidant, it is effective in preventing the aging of the skin as well as acting as a antiseptic and antiseptic. In this case, as the preservative, methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isobutyl paraben, and phenoxy ethanol are preferably used, and the weight ratio of the preservative to the aqueous solution is preferably 0.01 to 0.1% by weight.

If the amount of the preservative added is less than 0.01% by weight, it is difficult to prevent the deterioration of the gel as sterilization and preservatives, and when it is contained in more than 0.1% by weight, it is difficult to expect the improvement of the effect of increasing the content.

Step 2 of the manufacturing method is a step of forming the gel sheet by pouring the aqueous solution prepared in step 1 to the tray.

In the step 2, the aqueous solution obtained in the step 1 is poured into a tray and left to be molded at room temperature in the form of a gel sheet. When the temperature of the aqueous solution is lowered to 40 ° C. or lower, the gel is physically gelled due to the characteristics of the biocompatible polymer and the sheet is removed from the tray. Is formed. The tray in the present invention can be used in a general shape or a variety of sizes, thicknesses and shapes depending on the application, it is not limited thereto.

Step 3 of the manufacturing method is a step of irradiating the gel sheet formed in step 2, through which the polymer is crosslinked and sterilized at the same time.

By crosslinking the polymer in step 3 can be obtained a hydrogel of the desired physical properties, wherein the radiation used may be gamma rays, ultraviolet rays and electron beams.

In this case, the radiation dose is preferably 2 to 200 kGy. If less than 2 kGy can not be expected to form an effective cross-linking between the biocompatible polymers by irradiation, if it exceeds 200 kGy, the flexibility of the hydrogel is reduced due to the increase in the gel strength due to the increase in the amount of crosslinking, Radiation deterioration problem occurs.

The present invention also provides a gel for treating ulcers comprising honey prepared by the above method.

The ulcer gel for treating ulcers of the present invention has basic properties for use in wound healing, reduced water evaporation amount, prevention of infection from body fluid-absorbing bacteria, adhesion to wounds or skin, transparency, ease of handling, storage and sterilization properties. This has the effect of being applied as an antibiotic.

Hereinafter, the present invention will be described in more detail by the following examples.

However, the following examples merely illustrate the present invention, and the contents of the present invention are not limited by the following examples.

≪ Example 1 >

For treating ulcers Hydrated  Manufacturing 1

Step 1: As an biocompatible polymer, 20 g of sodium carboxymethyl cellulose, 10 g of chestnut honey, and 0.02 g of propyl paraben were dissolved in 69.98 g of purified water to prepare an aqueous solution.

Step 2: The aqueous solution prepared in Step 1 was poured into a tray and left at room temperature to form a hydrogel in a sheet form.

Step 3: The gel formed in Step 2 was irradiated with gamma rays to a total dose of 75 kGy to crosslink and sterilize the polymer.

<Example 2>

For treating ulcers Hydrated  Manufacture 2

Except for using honey instead of chestnut honey in step 1 of Example 1 was carried out in the same manner as in Example 1.

<Example 3>

For treating ulcers Hydrated  Manufacturing 3

Except for using acacia honey instead of chestnut honey in step 1 of Example 1 was carried out in the same manner as in Example 1.

<Example 4>

For treating ulcers Hydrated  Manufacturing 4

Except for using Manuka honey instead of chestnut honey in step 1 of Example 1 was carried out in the same manner as in Example 1.

&Lt; Comparative Example 1 &

Except for using propylene glycol instead of chestnut in step 1 of Example 1 was carried out in the same manner as in Example 1.

Comparative Example 2

Commercial Intrasite gel from Smith & Nephew was used.

<Experimental Example 1>

Gelation rate  analysis

In the present invention, the gel for treating ulcers prepared according to Examples 1 to 3 and the gel prepared according to Comparative Example 1 were cut to a size of 1.3 × 1.3 cm, and dried in a vacuum oven until they reached a constant weight. Samples were placed one by one in 100 ml bottles and filled with purified water. The bottles were placed in a shaking water bath and operated at 37 ° C. and 60 rpm for 489 hours, and then the samples were removed from the bottles and placed in a vacuum oven again until they reached a constant weight. The formula for calculating the gelation rate is the same as the following formula 1 and the results are shown in FIG.

&Quot; (1) &quot;

Gelation rate (%) = W _d / W _i × 100

W _d : weight of sample taken and dried

W _i : weight of the first dried sample

As shown in FIG. 1, the gelation rate of the gel prepared in Example 1 prepared using chestnut honey was the highest, and the gelation rate of the gel prepared using honey of the present invention was polyhydric alcohol. The gelation rate was higher than the gel using phosphorus polypropylene. Through this, it was confirmed that the gel for treating ulcers prepared using honey of the present invention has superior properties than gels prepared using conventional polyhydric alcohols.

<Experimental Example 2>

moisture Evaporation rate  Measure

In the present invention, the gel for treating ulcers prepared according to Examples 1 to 3 and the gel prepared according to Comparative Example 1 were placed in a Petri dish and placed in a constant temperature water bath controlled at 37 ° C. to measure the degree of moisture evaporation in the human body. The change in moisture evaporation rate was measured and the results are shown in FIG. 2.

As shown in FIG. 2, the gel prepared according to the embodiments of the present invention was able to confirm that water evaporation proceeded more slowly than the gel using propylene glycol, and in particular, prepared using chestnut honey prepared according to Example 1 of the present invention. The gel was confirmed that the rate of evaporation of the water proceeded most slowly.

Through this, it was found that the gel for treating ulcers containing honey of the present invention has an excellent effect than conventional gels in view of the purpose of treating ulcers.

<Experimental Example 3>

Compressive strength measurement

In the present invention, the gel for treating ulcers prepared according to Examples 1 to 3 and the gel prepared according to Comparative Example 1 were cut to a size of 10 × 10 × 15 mm in width × length × height and 50% shrinkage of the gel with Instron 4443. The compressive strength of the city was measured and the results are shown in FIG. 3.

As shown in FIG. 3, the compressive strength of the gel prepared using the chestnut honey prepared according to Example 1 of the present invention was the highest, and the gel prepared using the honey of the present invention was the polypropylene of Comparative Example 1. It was found to have a higher overall compressive strength value than the gel prepared using.

In addition, the gelation rate and the compressive strength shown in Experimental Example 1 was found to be proportional, and through this, it was confirmed that the mechanical properties of the ulcer gel containing honey of the present invention is excellent.

<Experimental Example 4>

Ulcer Treatment Animal Test Results

After anesthetizing the DB / DB mouse, the hair of the trochanter was cut and recuperated, and the ulcer was applied for 24 hours by applying a pressure of 140 mmHg for 24 hours using a 1 cm diameter stainless steel pressure rod as a pressure device. Caused. After washing the ulcer area with physiological saline, the ulcer treatment effect of the ulcer treatment paste prepared according to Examples 1 to 4 and the compounds of Comparative Examples 1 and 2 and mice undressed was observed. The results are shown in FIG. 3.

Ulcer treatment rate shown in Figure 3 was calculated by the following equation (2).

&Quot; (2) &quot;

As shown in FIG. 3, when the dressing treatment is not performed after the ulcer treatment, the ulcer area may be gradually treated after the ulcer area is increased. However, in the case of the gel prepared by Examples 1 to 4 of the present invention it can be seen that the ulcer area is reduced quickly. In addition, when compared with Comparative Examples 1 and 2 it was found that the ulcer treatment effect is faster, it was confirmed that the time until the ulcer is cured nearly 10 days.

Through this, it was confirmed that the gel for treating ulcers containing honey of the present invention has superior ulcer treatment efficacy than gels and commercial products using propylene glycol.

Claims (9)

Dissolving the biocompatible polymer, honey, and preservative in purified water to prepare an aqueous solution (step 1);
Pouring the aqueous solution of step 1 into a tray to form a gel sheet (step 2); And
Irradiating the gel sheet of step 2 to radiation (step 3), wherein the biocompatible polymer is a synthetic polymer selected from the group consisting of polyvinyl alcohol, polyvinylpyrrolidone, polyacrylic acid and polyethylene oxide or A method of producing a gel for treating ulcers, which is a natural polymer selected from the group consisting of carrageenan, sodium carboxymethyl cellulose, gelatin, agar, alginate and chitosan.
delete The method of claim 1, wherein the weight ratio of the biocompatible polymer is mixed in the step 1 is 1 to 50% by weight based on the total aqueous solution.
The method of claim 1, wherein the weight ratio of the honey is mixed in the step 1 is 1 to 30% by weight based on the total aqueous solution.
The method of claim 1, wherein the preservative of step 1 is selected from the group consisting of methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isobutyl paraben, and phenoxy ethanol.
The method of claim 1, wherein the weight ratio of the preservatives of step 1 is mixed is 0.01 to 0.1% by weight based on the total aqueous solution.
The method of claim 1, wherein the radiation of step 3 is selected from the group consisting of gamma rays, ultraviolet rays, and electron beams.
The method of claim 1, wherein the radiation dose of step 3 is 2 to 200 kGy, the method of producing a gel for treating ulcers.
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