KR20160148006A - 글리신 절단 시스템을 사용하는 코리네박테리아에서의 ｌ－아미노산의 생산 방법 - Google Patents

글리신 절단 시스템을 사용하는 코리네박테리아에서의 ｌ－아미노산의 생산 방법 Download PDF

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KR20160148006A
KR20160148006A KR1020167033695A KR20167033695A KR20160148006A KR 20160148006 A KR20160148006 A KR 20160148006A KR 1020167033695 A KR1020167033695 A KR 1020167033695A KR 20167033695 A KR20167033695 A KR 20167033695A KR 20160148006 A KR20160148006 A KR 20160148006A
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이네스 오흐롬벨
브리기테 바테
마를렌 하쎌마이어
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Abstract

코리네박테리움 휴미레듀센스 균주가 매우 효과적인 글리신 분열 시스템을 포함한다는 것이 놀랍게도 발견되었다.

Description

글리신 절단 시스템을 사용하는 코리네박테리아에서의 Ｌ－아미노산의 생산 방법 {METHOD FOR PRODUCING L-AMINO ACIDS IN CORYNEBACTERIA USING A GLYCINE CLEAVAGE SYSTEM}
본 발명은 글리신 절단 시스템을 사용하는 코리네박테리아에서의 L-아미노산의 생산 방법에 관한 것이다.
코리네박테리움 (Corynebacterium) 속의 박테리아를 사용하는 L-아미노산의 생산 방법은 당업자에게 공지되어 있다.
다수의 코리네박테리움 종이 공지되어 있지만, 이러한 방법에서 코리네박테리움 글루타미쿰 (Corynebacterium glutamicum) 종의 박테리아가 일반적으로 이용되는데, 이러한 종이 L-아미노산의 생산에 특히 유리한 것으로 발견되었기 때문이다.
또한, 글리신 절단 시스템 (GCV)을 사용함으로써 L-아미노산의 수율을 더욱 증가시킬 수 있다는 것이 밝혀졌는데, 이는 원하지 않는 L-글리신 부산물의 형성이 글리신 절단 시스템에 의해 크게 방지될 수 있기 때문이다. L-글리신의 생성 억제 또는 잉여 L-글리신의 분해는 특히 L-글리신이 등몰 부산물로서 생성되기 때문에 L-메티오닌의 생산에서 특히 중요하다.
글리신 절단 시스템은 2 개 이상의 서브 유닛, 즉 서브 유닛 GcvP, GcvT 및 GcvH 로 구성된다. 이는 글리신의 이산화탄소, 암모늄 이온 및 N5-10-메틸렌 테트라히드로폴레이트로의 산화적 탈카르복실화 및 탈아민화를 촉매하는 다중-효소 복합체의 형태를 취한다.
글리신 디히드로게나아제 GcvP 는 이산화탄소를 유리시켜 피리독살 인산염에 결합된 아미노 메틸 화합물을 남기는 피리독살 인산-함유 디카르복실라아제이다.
H-단백질 GcvH 는 리포아마이드-함유 아미노메틸트랜스페라아제이다.
효소 GcvT 는 N5-10-메틸렌테트라하이드로폴레이트를 형성하는 테트라하이드로폴레이트에 의한 아미노메틸리포아마이드에 대한 친핵성 공격을 촉매하고 완전히 환원된 리포아마이드가 GcvH 에 결합되어 있는 암모늄 이온을 유리시킨다.
글리신 절단 시스템은 모든 코리네박테리아에 존재하지 않지만, 현재까지 매우 적은 코리네박테리아만이 보고되었다. 예를 들어, 아미노산 생산에 특히 바람직한 생산 균주인 C. 글루타시움은 예를 들어, 고유의 글리신 절단 시스템을 갖지 않는다. C. 글루타미쿰에 대한 글리신 절단 시스템의 이점을 이용하기 위해, 이러한 시스템은 또 다른 코리네박테리움에서 C. 글루타미쿰으로 혼입되어야 하고 이종으로 발현되어야 한다. C. 제이케이움의 글리신 절단 시스템 (WO 2008/101857) 이 이러한 목적을 위해 현재까지 사용되어왔다. 이 경우의 심각한 단점은 C.제이케이움이 병원성 유기체라는 것이다. 추가의 단점은 원하지 않는 글리신 부산물의 분해를 위해 이종 구조체가 제조되어야 한다는 것이다.
따라서, 본 발명의 주요 목적은 새로운 글리신 절단 시스템, 바람직하게는 병원성 유기체에서 유래하지 않는 글리신 절단 시스템을 제공하는 것이며, 이때 글리신 절단 시스템은 바람직하게는 다른 코리네박테리아, 특히, C. 글루타미쿰으로의 혼입에 적합하다.
본 발명의 또 다른 목적은 L-아미노산을 본질적으로 생산할 수 있으며, 또한 C. 글루타미쿰의 경우에서와 같이 이종 구조체의 제조가 필요하지 않도록 하는 고유의 글리신 절단 시스템을 갖는 코리네박테리움을 제공하는 것이다.
놀랍게도, 비병원성인 코리네박테리움 휴미레듀센스 종은 현재까지 기술된 글리신 절단 시스템과 구조적으로 상당히 상이한 고유의 글리신 절단 시스템을 갖는다는 것이 본 발명에 따라 발견되었다.
놀랍게도, 코리네박테리움 휴미레듀센스의 글리신 절단 시스템은 아미노산 합성에서 원하지 않는 부산물인 글리신이 비교적 적은 양으로 세포 내에 축적되는 것과 같이 매우 효과적이며 (야생형 균주에서는 전혀 축적되지 않음), 본 발명에 따른 글리신 분해 시스템의 증폭에 의해, 특히 과발현에 의해, 글리신 부산물 형성의 억제가 상응하게 더 개선될 수 있다. 특히, 글리신 부산물 형성은 또한 다른 미생물, 특히 C. 글루타미쿰에서의 본 발명에 따른 글리신 분해 시스템의 과발현에 의해 효과적으로 억제될 수 있다.
놀랍게도, C. 휴미레듀센스가 특히 숙주 글리신 절단 시스템을 갖는 추가의 생산 균주 개발을 위한 출발점으로 사용될 수 있는 상동 글리신 절단 시스템을 갖는 L-아미노산 생산 균주인 것으로, C. 휴미레듀센스가 본질적으로 L-아미노산, 특히 L-알라닌, L-발린 및 L-글루탐산을 생산할 수 있음이 밝혀졌다.
C. 휴미레듀센스 균주는, Wu 등에 의해 처음으로 기재된다 (International Journal of Systematic and Evolutionary Microbiology (2011), 61, 882-887). 상기 균주는 침착수 DSM 45392 하, DSMZ 에서 침착되었고, 이의 16S rRNA 는 EMBL 에서 침착되었고, 수탁 번호 GQ421281 을 갖는다. 모균주는 내염, 호알칼리성, 휴민산-환원 박테리아이다.
C. 휴미레듀센스에 관한 추가 정보가 하기 출판에서 발견된다: Wu 등. (Microb. Biotechnol. (2013), 6(2), 141-149), Lin 등. (Bioresour. Technol. (2013), 136, 302-308).
따라서, 본 발명의 제 1 목적은 하기로 이루어진 군으로부터 선택되는 글리신 절단 시스템의 효소에 관한 것이다:
a) SEQ ID NO: 40 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvP,
b) SEQ ID NO: 42 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvT,
c) SEQ ID NO: 38 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvH.
따라서, 본 발명은 또한 효소 GcvP, GcvT 및 GcvH 를 포함하는 글리신 절단 시스템에 관한 것으로서, 상기 시스템이 하기 폴리펩티드 중 적어도 하나를 포함하는 것을 특징으로 한다:
a) SEQ ID NO: 40 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvP,
b) SEQ ID NO: 42 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvT,
c) SEQ ID NO: 38 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvH.
이 경우, 본 발명에 따른 글리신 절단 시스템은 바람직하게는 상기 언급한 폴리펩티드 (a) 내지 (c) 중 적어도 2 개, 바람직하게는 3 개 모두를 포함한다.
하기 3 개의 폴리펩티드를 포함하는 글리신 절단 시스템이 특히 바람직하다:
a) SEQ ID NO: 40 에 따른 서열에 대해, 적어도 95 또는 98% 의 서열 동일성을 갖는 효소 GcvP,
b) SEQ ID NO: 42 에 따른 서열에 대해, 적어도 95 또는 98% 의 서열 동일성을 갖는 효소 GcvT,
c) SEQ ID NO: 38 에 따른 서열에 대해, 적어도 95 또는 98% 의 서열 동일성을 갖는 효소 GcvH.
보다 넓은 의미에서, 효소 LpdA, LplA, LipA, LipB 및 GcvH 는 또한 글리신 절단 시스템에 속한다.
디히드로리포아미드 디히드로게나아제 (LpdA) 는 GcvH 에 결합된 리포아미드를 재산화한다.
리포에이트-단백질 리가아제 A (LplA) 는 GcvH 의 리포일화를 촉매한다.
리포산 신타아제 (LipA) 는 리포산의 합성을 촉매한다.
리포일-[아실 캐리어 단백질]-단백질 N-리포일트랜스페라아제 (LipB) 는 GcvH 로의 리포산의 전이를 촉매한다.
GcvL 은 디히드로리포일 디히드로게나아제이다.
따라서, 바람직한 구현예에서, 본 발명에 따른 글리신 절단 시스템은 하기로 이루어진 군으로부터 선택되는 적어도 하나의 추가 효소를 추가로 포함한다:
a) 효소 LipA, 바람직하게는 SEQ ID NO: 48 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LipA,
b) 효소 LipB, 바람직하게는 SEQ ID NO: 50 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LipB,
c) 효소 Lpd, 바람직하게는 SEQ ID NO: 52 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 Lpd,
d) 효소 LplA, 바람직하게는 SEQ ID NO: 94 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LplA,
e) 효소 GcvL, 바람직하게는 SEQ ID NO: 96 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvL.
본 발명에 따른 특히 바람직한 구현예에서, 본 발명에 따른 글리신 절단 시스템은 이 경우 상기 언급한 폴리펩티드 중 적어도 2, 3 또는 4 개, 바람직하게는 5 개 모두를 포함한다.
따라서, 본 발명은 또한 하기로 이루어진 군으로부터 선택되는 효소에 관한 것이다:
a) 효소 LipA, 바람직하게는 SEQ ID NO: 48 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LipA,
b) 효소 LipB, 바람직하게는 SEQ ID NO: 50 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LipB,
c) 효소 Lpd, 바람직하게는 SEQ ID NO: 52 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 Lpd,
d) 효소 LplA, 바람직하게는 SEQ ID NO: 94 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LplA,
e) 효소 GcvL, 바람직하게는 SEQ ID NO: 96 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvL.
리포산 및/또는 리포아미드의 적절한 합성을 보장하는 적절한 효소의 사용에 대한 대안으로서 또는 이에 부가하여, 이들 화합물은 또한 예를 들어 15 mM 이하의 양으로 반응 매질에 첨가될 수 있다.
본 발명은 또한 추가로 본 발명에 따른 효소를 코딩하는 뉴클레오티드 및/또는 본 발명에 따른 글리신 절단 시스템에 관한 것이다. 이러한 폴리뉴클레오티드는 바람직하게는 하기로 이루어진 군으로부터 선택된다:
a) 효소 GcvP 를 코딩하고, SEQ ID NO: 39 에 따른 위치 301 내지 3162 의 서열에 대해, 적어도 70 또는 75%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 39 에 따른 위치 301 내지 3162 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvP);
b) 효소 GcvT 를 코딩하고, SEQ ID NO: 41 에 따른 위치 301 내지 1404 의 서열에 대해, 적어도 70 또는 75%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 41 에 따른 위치 301 내지 1404 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvT);
c) 효소 GcvH 를 코딩하고, SEQ ID NO: 37 에 따른 위치 301 내지 699 의 서열에 대해, 적어도 70 또는 75%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 37 에 따른 위치 301 내지 699 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvH);
d) 효소 LipA 를 코딩하고, SEQ ID NO: 47 에 따른 위치 301 내지 1344 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 47 에 따른 위치 301 내지 1344 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lipA);
e) 효소 LipB 를 코딩하고, SEQ ID NO: 49 에 따른 위치 301 내지 1089 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 49 에 따른 위치 301 내지 1089 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lipB);
f) 효소 Lpd 를 코딩하고, SEQ ID NO: 51 에 따른 위치 301 내지 1710 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 51 에 따른 위치 301 내지 1710 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lpd);
g) 효소 LplA 를 코딩하고, SEQ ID NO: 93 에 따른 위치 301 내지 1089 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 93 에 따른 위치 301 내지 1089 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lplA);
h) 효소 GcvL 를 코딩하고, SEQ ID NO: 95 에 따른 위치 301 내지 1710 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 95 에 따른 위치 301 내지 1710 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvL).
본 발명에 있어서, "엄격한 조건" 은 80℃ 의 온도에서 1 x SSC 및 0.1 중량% SDS 의 염 농도에서 세척하는 것을 의미하는 것으로 이해된다.
마찬가지로, 본 발명은 또한 상기 언급한 코딩 폴리뉴클레오티드에 상보적인 폴리뉴클레오티드에 관한 것이다.
본 발명은 또한 추가로 본 발명에 따른 적어도 하나의 폴리뉴클레오티드를 포함하는 적절한 벡터, 특히 클로닝 및 발현 벡터에 관한 것이다. 이러한 벡터는 미생물, 특히 코리네형 박테리아, 특히 코리네박테리움 또는 엔테로박테리아세아에 (Enterobacteriaceae) 속, 특히 에스케리키아 (Escherichia) 속의 미생물 내로 적절히 혼입될 수 있다.
본 발명에 따른 벡터는 본 발명에 따른 하나 이상의 폴리뉴클레오티드를 포함할 수 있다. 본 발명에 따른 바람직한 벡터는 GcvP, GcvT 및 GcvH 로부터 선택되는 본 발명에 따른 효소를 코딩하는 적어도 하나의 폴리 폴리뉴클레오티드를 포함한다. 특히 바람직한 벡터는 본 발명에 따른 세 가지 효소 GcvP, GcvT 및 GcvH 를 모두 코딩하는 폴리뉴클레오티드를 포함한다.
본 발명에 따른 벡터는 바람직하게는 적합한 프로모터 및/또는 적합한 프로모터들 및 임의로 재조합 박테리움, 바람직하게는 재조합 코리네박테리움에서 본 발명의 폴리뉴클레오티드의 발현을 가능하게 하는 추가의 조절 요소를 갖는다.
뿐만 아니라, 코딩된 유전자의 발현을 위해, 본 발명에 따른 폴리뉴클레오티드는 또한 미생물의 게놈, 특히 코리네형 박테리아의 게놈, 특히 코리네박테리움 속의 게놈, 또는 특히 엔테로박테리아세아에의 게놈, 특히 에스케리키아 속의 게놈 내로 혼입될 수 있다.
또한, 본 발명은 본 발명에 따른 적어도 하나의 효소 및/또는 본 발명에 따른 적어도 하나의 폴리뉴클레오티드 및/또는 본 발명에 따른 적어도 하나의 벡터 및/또는 본 발명에 따른 하나의 글리신 절단 시스템 및/또는 본 발명에 따른 글리신 절단 시스템을 코딩하는 폴리뉴클레오티드를 포함하는, 상응하는 재조합 미생물, 바람직하게는 박테리아, 특히 코리네형 박테리아, 특히 코리네박테리움 속 박테리아, 특히 바람직하게는 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 박테리아, 및 또한 엔테로박테리아세아에, 특히 에스케리키아 속 박테리아에 관한 것이다.
이러한 맥락에서, 바람직한 목적은, 본 발명에 따른 적어도 하나의 효소, 바람직하게는 GcvP, GcvT 및 GcvH 로부터 선택되는 본 발명에 따른 모든 효소 및/또는 상기 효소를 코딩하는 폴리뉴클레오티드 및/또는 상기 폴리뉴클레오티드를 포함하는 적어도 하나의 벡터를 포함하는 재조합 코리네박테리아, 특히 C. 휴미레듀센스 종 및 C. 글루타미쿰 종의 코리네박테리아이다.
본 발명은 또한, 특히, 본 발명에 따른 적어도 하나의 효소 및/또는 본 발명에 따른 적어도 하나의 폴리뉴클레오티드 및/또는 본 발명에 따른 적어도 하나의 벡터 및/또는 본 발명에 따른 하나의 글리신 절단 시스템 및/또는 본 발명에 따른 글리신 절단 시스템을 코딩하는 폴리뉴클레오티드를 포함하는 재조합 미생물, 바람직하게는 박테리아, 특히 코리네형 박테리아, 특히 C. 휴미레듀센스 종을 제외한 코리네박테리움 속 박테리아, 특히 C. 글루타미쿰 종 박테리아에 관한 것이다.
이러한 맥락에서, 바람직한 목적은, 본 발명에 따른 적어도 하나의 효소, 바람직하게는 GcvP, GcvT 및 GcvH 로부터 선택되는 본 발명에 따른 모든 효소 및/또는 상기 효소를 코딩하는 폴리뉴클레오티드 및/또는 상기 폴리뉴클레오티드를 포함하는 적어도 하나의 벡터를 포함하는 C. 휴미레듀센스 종을 제외한, 재조합 코리네박테리아, 특히 C. 글루타미쿰 종의 박테리아이다.
본 발명에 있어서, "재조합 미생물" 또는 "재조합 박테리아" 는 적어도 하나의 유전 공학 조치를 받았던 미생물 또는 박테리아를 의미하는 것으로 이해된다. 이러한 맥락에서, 유전 공학 조치는, 특히 표적화되거나 무작위 돌연변이, 외래 유전자의 혼입 및/또는 숙주 유전자 또는 외래 유전자의 과발현 또는 감쇠일 수 있다. 본 발명에 따른 재조합 미생물 또는 본 발명에 따른 재조합 박테리아는 바람직하게는 적어도 하나의 유전자의 과발현 또는 감쇠에 의해 특징화된다. 특히 바람직한 구현예에서, 본 발명에 따른 재조합 미생물 또는 본 발명에 따른 재조합 박테리아는 본 발명에 따른 적어도 하나의 효소 및/또는 상기 효소를 코딩하는 폴리뉴클레오티드의 및/또는 본 발명에 따른 글리신 절단 시스템 또는 상기 시스템을 코딩하는 폴리뉴클레오티드의 과발현에 의해 특징화된다.
글리신 형성에 관하여, "상대적으로 적은 양" 은 각각의 경우 발효 완료 후 세포 및/또는 발효 배지에 축적된 글리신 함량을 기준으로 0.3 g/l 이하, 바람직하게는 0.2 또는 0.1 g/l 이하, 특히 바람직하게는 0.05 이하 또는 0.03g/l 이하의 양을 의미하는 것으로 이해된다.
코리네박테리움 속에서, 바람직한 것은 하기 종을 기반으로 하는 본 발명에 따른 균주이다: 코리네박테리움 에피시엔스 (efficiens), 예컨대 DSM44549 형 균주, 코리네박테리움 글루타미쿰, 예컨대 ATCC13032 형 균주 또는 R 형 균주, 코리네박테리움 암모니아유전자, 예컨대 ATCC6871 형 균주, 코리네박테리움 휴미레듀센스, 예컨대 DSM 45392 균주, 및 코리네박테리움 페키네즈 (pekinese), 예컨대 CGMCC No. 5361 균주.
특히 바람직한 것은 코리네박테리움 글루타미쿰 및 코리네박테리움 휴미레듀센스 종이다. 본 출원의 맥락에서, 코리네박테리움 휴미레듀센스 균주가 언급되는 경우, 상기 균주는 바람직하게는 DSM 45392 균주 또는 이로부터 유래된 균주이다.
몇몇 대표적인 코리네박테리움 글루타미쿰 종은 또한 다른 이름으로 선행 기술에서 알려져 있다. 이의 예는, 하기를 포함한다: 코리네박테리움 아세토아시도필룸 ATCC13870, 코리네박테리움 릴리움 DSM20137, 코리네박테리움 멜라세콜라 ATCC17965, 브레비박테리아 플라붐 ATCC14067, 브레비박테리아 락토페르멘툼 ATCC13869 및 브레비박테리아 디바리카툼 ATCC14020. 코리네박테리움 글루타미쿰에서의 용어 "미크로코쿠스 글루타미쿠스 (Micrococcus glutamicus)" 가 마찬가지로 사용되었다. 몇몇 대표적인 코리네박테리움 에피시엔스 종은 또한 코리네박테리움 써모아미노유전자 (thermoaminogene) 로서 선행 기술에서 지칭되었다 (예를 들어, FERM BP-1539 균주).
코리네형 박테리아의 군 균주의 분류학적 분류에 대한 정보는, 특히 Seiler (Journal of General Microbiology 129, 1433-1477 (1983)), Kinoshita (1985, Glutamic Acid Bacteria, p 115-142. in: Demain and Solomon (ed), Biology of Industrial Microorganisms. The Benjamin/Cummins Publishing Co., London, UK), Kampfer 및 Kroppenstedt (Canadian Journal of Microbiology 42, 989-1005 (1996)), Liebl 등 (International Journal of Systematic Bacteriology 41, 255-260 (1991)), Fudou 등 (International Journal of Systematic and Evolutionary Microbiology 52, 1127-1131 (2002)) 및 US-A-5,250,434 에서 확인될 수 있다.
명칭 "ATCC" 를 갖는 균주는, American Type Culture Collection (Manassas, VA, USA) 로부터 수득될 수 있다. 명칭 "DSM" 을 갖는 균주는, Deutschen Sammlung von Mikroorganismen und Zellkulturen (German Microorganism and Cell Culture collection) (DSMZ, Braunschweig, Germany 로부터 수득될 수 있다. 명칭 "NRRL" 을 갖는 균주는, Agricultural Research Service Patent Culture Collection (ARS, Peoria, Illinois, US) 로부터 수득될 수 있다. 명칭 "FERM" 을 갖는 균주는, National Institute of Advanced Industrial Science 및 Technology (AIST Tsukuba Central 6, 1-1-1 Higashi, Tsukuba Ibaraki, Japan) 로부터 수득될 수 있다. 명칭 "CGMCC" 를 갖는 균주는, China General Microbiological Culture Collection Center (CGMCC, Beijing, China) 로부터 수득될 수 있다.
본 발명은 또한 추가로 L-아미노산의 과잉생산 방법에 관한 것으로서, 본 발명에 따른 적어도 하나의 효소 및/또는 상기 효소를 코딩하는 폴리뉴클레오티드 및/또는 본 발명에 따른 글리신 절단 시스템 및/또는 상기 시스템을 코딩하는 폴리뉴클레오티드 및/또는 본 발명에 따른 재조합 미생물, 바람직하게는 본 발명에 따른 재조합 박테리아, 특히 본 발명에 따른 재조합 코리네형 박테리아, 특히 바람직하게는 본 발명에 따른 재조합 코리네박테리움, 특히 C. 휴미레듀센스 종 또는 C. 글루타미쿰 종의 코리네박테리움이 상기 방법에서 사용되는 것을 특징으로 한다. 본 발명에 따른 바람직한 구현예에서, 본 발명에 따른 적어도 하나의 폴리뉴클레오티드는 이 경우에 과발현 형태로 사용된다.
이 경우 본 발명은 바람직하게는 효소 GcvP, GcvT 및 GcvH 를 포함하는 글리신 절단 시스템이 상기 방법에서 사용되는 것을 특징으로 하는, L-아미노산의 과잉 생산 방법에 관한 것이며, 글리신 절단 시스템은 본 발명에 따른 효소 GcvP, GcvT 및 GcvH 중 적어도 하나 및/또는 글리신 절단 시스템의 효소 GcvP, GcvT 및 GcvH 를 코딩하는 폴리뉴클레오티드를 포함하고, 상기 폴리뉴클레오티드는 본 발명에 따른 폴리뉴클레오티드 gcvP, gcvT 및 gcvH 중 적어도 하나를 포함하고, 및/또는 GcvP, GcvT 및 GcvH 로부터 선택되는, 본 발명에 따른 적어도 하나의 효소, 바람직하게는 3 개 모두의 효소 및/또는 gcvP, gcvT 및 gcvH 로부터 선택되는, 본 발명에 따른 적어도 하나의 폴리뉴클레오티드, 바람직하게는 본 발명에 따른 3 개 모두의 폴리뉴클레오티드를 포함하는, 재조합 코리네박테리움, 바람직하게는 C. 휴미레듀센스 또는 C. 글루타미쿰의 종의 코리네박테리움이 사용된다.
이 경우 본 발명은 특히 바람직하게는 글리신 절단 시스템이 방법에서 사용되는 것을 특징으로 하는 L-아미노산의 과잉생산 방법에 관한 것이며, 글리신 절단 시스템이 본 발명에 따른 효소 GcvP, GcvT 및 GcvH 및/또는 상기 효소를 코딩하는 폴리뉴클레오티드를 포함하고, 및/또는 상기 방법에서 이러한 글리신 절단 시스템 및/또는 상기 시스템을 코딩하는 폴리뉴클레오티드를 포함하는, 재조합 박테리아, 바람직하게는 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 재조합 박테리아가 사용된다.
본 발명에 따른 "L-아미노산" 은, 특히 단백질생성성 (proteinogenic) L-아미노산을 의미하는 것으로 이해된다.
이러한 경우, L-아미노산은 바람직하게는 L-알라닌, L-발린, 글루타메이트 패밀리의 L-아미노산, 특히 L-글루타메이트, L-글루타민, L-프롤린 및 L-아르기닌, 및 아스파르테이트 패밀리의 L-아미노산, 특히 L-아스파르테이트, L-아스파라긴, L-메티오닌, L-리신, L-이소류신 및 L-트레오닌으로부터 선택되고, 특히 바람직하게는 L-알라닌, L-발린, L-글루타메이트, L-메티오닌, L-리신 및 L-트레오닌으로부터 선택된다. L-메티오닌이 특히 바람직하다.
본 발명에 있어서, L-아미노산의 과잉생산은 바람직하게는 C. 휴미레듀센스 또는 C. 글루타미쿰에서 시행된다.
본 발명에 따른 방법은 바람직하게는 단지 적은 양의 글리신이 부산물로서 발생한다는 것을 특징으로 한다. 본 발명에 따른 방법에서, 글리신은 바람직하게는 0.2 g/l 미만, 특히 0.1 g/l 미만, 특히 바람직하게는 0.05 g/l 미만의 양으로 발생한다.
본 발명에 있어서, L-아미노산에 관해 "과잉생산하다" 또는 "과잉생산" 은, 미생물이 세포 내에서 농축되거나, 이들이 축적되는 주변 영양 배지 내로 분비되는 이의 자체 요건에 따라, L-아미노산을 생산하는 것을 의미하는 것으로 이해된다. 이러한 경우, 미생물은 바람직하게는 세포 또는 영양 배지에서 농축되거나 축적될 수 있다 (L-아미노산에 대해 ≤ (최대) 120 시간, ≤ 96 시간, ≤ 48 시간, ≤ 36 시간, ≤ 24 시간 또는 ≤ 12 시간 중에 ≥(적어도) 0.25 g/l, ≥ 0.5 g/l, ≥ 1.0 g/l, ≥ 1.5 g/l, ≥ 2.0 g/l, ≥ 4 g/l 또는 ≥ 10 g/l).
바람직한 구현예에서, 본 발명에 따른 폴리뉴클레오티드 및/또는 본 발명에 따른 벡터가 혼입된, 본 발명에 따른 재조합 미생물은, 본 발명에 따른 폴리뉴클레오티드 및/또는 벡터의 혼입 전에 이미 L-아미노산을 과잉생산할 수 있다. 바람직하게는, 개시 균주는 돌연변이 유발 및 선별에 의해, 재조합 DNA 기술 또는 두 방법의 조합에 의해 생산된 균주이다.
본 발명에 따른 폴리뉴클레오티드 및/또는 본 발명에 따른 벡터가 존재하거나 혼입된 야생 균주에서, 본 발명에 따른 재조합 미생물이 이에 따라 또한 생산될 수 있고, 적합한 후속의 추가 유전 공학 조치에 의해 L-아미노산이 생산되거나 L-아미노산 생산이 증가되는 것은 명백하고, 추가의 설명을 필요로 하지 않는다.
본 발명은 또한 추가로 C. 휴미레듀센스로부터의 다른 폴리뉴클레오티드 및 또한 상기 폴리뉴클레오티드에 의해 코딩된 폴리펩티드에 관한 것이다. 관련 폴리뉴클레오티드 또는 폴리펩티드의 과발현에 의해, 특정 L-아미노산의 아미노산 생산은 긍정적인 영향을 받을 수 있다.
따라서, 본 발명은 마찬가지로 다음에 관한 것이다:
a) SEQ ID NO: 106 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트레오닌 디히드라타아제 (IlvA, EC 4.3.1.19) 및 이를 코딩하는 폴리뉴클레오티드,
b) SEQ ID NO: 98 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아세토락테이트 신타아제의 서브유닛 (IlvB) 및 이를 코딩하는 폴리뉴클레오티드,
c) SEQ ID NO: 100 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소메로리덕타아제 (IlvC, EC 1.1.1.86) 및 이를 코딩하는 폴리뉴클레오티드,
d) SEQ ID NO: 102 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디히드록시산 디히드라타아제 (IlvD, EC 4.2.1.9) 및 이를 코딩하는 폴리뉴클레오티드,
e) SEQ ID NO: 104 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트랜스아미나아제 (IlvE, EC 2.6.1.42) 및 이를 코딩하는 폴리뉴클레오티드,
f) SEQ ID NO: 122 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아세토락테이트 신타아제 (IlvH, EC 2.2.1.6) 및 이를 코딩하는 폴리뉴클레오티드,
g) SEQ ID NO: 118 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 3-메틸-2-옥소부타노에이트 히드록시메틸트랜스페라아제 (PanB, EC 2.1.2.11) 및 이를 코딩하는 폴리뉴클레오티드,
h) SEQ ID NO: 120 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 판토테네이트 신타아제 (PanC, EC 6.3.2.1) 및 이를 코딩하는 폴리뉴클레오티드,
i) SEQ ID NO: 124 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타메이트 디히드로게나아제 (Gdh) 및 이를 코딩하는 폴리뉴클레오티드,
j) SEQ ID NO: 126 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타민 신테타아제 (글루타민 신테타아제 1) 및 이를 코딩하는 폴리뉴클레오티드,
k) SEQ ID NO: 128 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타민 신테타아제 (글루타민 신테타아제 2) 및 이를 코딩하는 폴리뉴클레오티드,
l) SEQ ID NO: 130 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타메이트 신타아제 및 이를 코딩하는 폴리뉴클레오티드,
m) SEQ ID NO: 132 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소시트레이트 디히드로게나아제 및 이를 코딩하는 폴리뉴클레오티드,
n) SEQ ID NO: 134 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아코니테이트 히드라아제 및 이를 코딩하는 폴리뉴클레오티드,
o) SEQ ID NO: 136 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시트레이트 신타아제 및 이를 코딩하는 폴리뉴클레오티드,
p) SEQ ID NO: 138 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아미노펩티다아제 C (PepC) 및 이를 코딩하는 폴리뉴클레오티드,
q) SEQ ID NO: 140 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 디히드로게나아제 및 이를 코딩하는 폴리뉴클레오티드,
r) SEQ ID NO: 142 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 키나아제 (피루베이트 키나아제 1) 및 이를 코딩하는 폴리뉴클레오티드,
s) SEQ ID NO: 144 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 키나아제 (피루베이트 키나아제 2) 및 이를 코딩하는 폴리뉴클레오티드,
t) SEQ ID NO: 146 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 에놀라아제 및 이를 코딩하는 폴리뉴클레오티드,
u) SEQ ID NO: 148 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 2,3-비스포스포글리세레이트-의존성 포스포글리세레이트 뮤타아제 (GpmA) 및 이를 코딩하는 폴리뉴클레오티드,
v) SEQ ID NO: 150 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포글리세레이트 키나아제 (Pgk) 및 이를 코딩하는 폴리뉴클레오티드,
w) SEQ ID NO: 152 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글리세르알데히드-3-포스페이트 디히드로게나아제 (글리세롤-3-포스페이트 디히드로게나아제 1) 및 이를 코딩하는 폴리뉴클레오티드,
x) SEQ ID NO: 154 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글리세르알데히드-3-포스페이트 디히드로게나아제 (글리세롤-3-포스페이트 디히드로게나아제 2) 및 이를 코딩하는 폴리뉴클레오티드,
y) SEQ ID NO: 156 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트리오스포스페이트 이소메라아제 (TpiA) 및 이를 코딩하는 폴리뉴클레오티드,
z) SEQ ID NO: 158 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 프럭토오스 비스포스페이트 알돌라아제 및 이를 코딩하는 폴리뉴클레오티드,
aa) SEQ ID NO: 160 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 1-포스포프럭토키나아제 및 이를 코딩하는 폴리뉴클레오티드,
bb) SEQ ID NO: 162 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 6-포스포프럭토키나아제 및 이를 코딩하는 폴리뉴클레오티드,
cc) SEQ ID NO: 4 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 키나아제 (ThrB, EC 2.7.1.39) 및 이를 코딩하는 폴리뉴클레오티드,
dd) SEQ ID NO: 22 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스테인 신타아제 (CBS, CysK) 및 이를 코딩하는 폴리뉴클레오티드,
ee) SEQ ID NO: 26 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 베타-리아제 (AecD) 및 이를 코딩하는 폴리뉴클레오티드,
ff) SEQ ID NO: 28 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 세미알데히드 디히드로게나아제 (Asd, EC 1.2.1.11) 및 이를 코딩하는 폴리뉴클레오티드,
gg) SEQ ID NO: 30 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 분지쇄 아미노산에 대한 운반체의 보다 작은 서브유닛 (BrnE) 및 이를 코딩하는 폴리뉴클레오티드,
hh) SEQ ID NO: 32 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 분지쇄 아미노산에 대한 운반체의 보다 큰 서브유닛 (BrnF) 및 이를 코딩하는 폴리뉴클레오티드,
ii) SEQ ID NO: 34 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 세린 아세틸트랜스페라아제 (CysE) 및 이를 코딩하는 폴리뉴클레오티드,
jj) SEQ ID NO: 36 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스테인 신타아제 (CysK) 및 이를 코딩하는 폴리뉴클레오티드,
kk) SEQ ID NO: 44 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 세린 히드록시메틸트랜스페라아제 (GlyA) 및 이를 코딩하는 폴리뉴클레오티드,
ll) SEQ ID NO: 46 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 임의적 피드백-내성 호모세린 디히드로게나아제 (Hom, EC 1.2.1.11) 및 이를 코딩하는 폴리뉴클레오티드,
mm) SEQ ID NO: 54 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 임의적 피드백-내성 아스파르테이트 키나아제 (LysC, EC 2.7.2.4) 및 이를 코딩하는 폴리뉴클레오티드,
nn) SEQ ID NO: 56 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 감마-신타아제 (MetB) 및 이를 코딩하는 폴리뉴클레오티드,
oo) SEQ ID NO: 58 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 5,10-메틸렌테트라히드로폴레이트 리덕타아제 (MetF) 및 이를 코딩하는 폴리뉴클레오티드,
pp) SEQ ID NO: 60 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 O-아세틸트랜스페라아제 (MetX) 및 이를 코딩하는 폴리뉴클레오티드,
qq) SEQ ID NO: 62 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 O-아세틸호모세린 리아제 (MetY) 및 이를 코딩하는 폴리뉴클레오티드,
rr) SEQ ID NO: 64 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 임의적 피드백-내성인 피루베이트 카르복실라아제 (Pyc, EC 6.4.1.1) 및 이를 코딩하는 폴리뉴클레오티드,
ss) SEQ ID NO: 66 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 임의적 피드백-내성 D-3-포스포글리세레이트 디히드로게나아제 (SerA) 및 이를 코딩하는 폴리뉴클레오티드,
tt) SEQ ID NO: 68 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포세린 포스파타아제 (SerB) 및 이를 코딩하는 폴리뉴클레오티드,
uu) SEQ ID NO: 70 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포세린 아미노트랜스페라아제 (SerC) 및 이를 코딩하는 폴리뉴클레오티드,
vv) SEQ ID NO: 74 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술페이트 아데닐릴트랜스페라아제의 서브유닛 (CysD) 및 이를 코딩하는 폴리뉴클레오티드,
ww) SEQ ID NO: 76 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아데노신 포스포술페이트 리덕타아제 (CysH) 및 이를 코딩하는 폴리뉴클레오티드,
xx) SEQ ID NO: 78 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술파이트 리덕타아제 (CysI) 및 이를 코딩하는 폴리뉴클레오티드,
yy) SEQ ID NO: 80 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 NADPH-의존성 글루타메이트 신타아제 베타 사슬 (CysJ) 및 이를 코딩하는 폴리뉴클레오티드,
zz) SEQ ID NO: 82 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술페이트 아데닐릴트랜스페라아제의 큰 서브유닛 (CysN) 및 이를 코딩하는 폴리뉴클레오티드,
aaa) SEQ ID NO: 84 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 베타-신타아제 (CysY) 및 이를 코딩하는 폴리뉴클레오티드,
bbb) SEQ ID NO: 86 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술페이트 운반체 (CysZ) 및 이를 코딩하는 폴리뉴클레오티드,
ccc) SEQ ID NO: 88 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 5-메틸테트라히드로프테로일트리글루타메이트-호모시스테인 메틸트랜스페라아제 (MetE) 및 이를 코딩하는 폴리뉴클레오티드,
ddd) SEQ ID NO: 90 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 펩티딜-tRNA 히드롤라아제 1 (PtH1) 및 이를 코딩하는 폴리뉴클레오티드,
eee) SEQ ID NO: 92 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 펩티딜-tRNA 히드롤라아제 2 (PtH2) 및 이를 코딩하는 폴리뉴클레오티드,
fff) SEQ ID NO: 202 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디아미노피멜레이트 디히드로게나아제 (Ddh, EC 1.4.1.16) 및 이를 코딩하는 폴리뉴클레오티드,
ggg) SEQ ID NO: 164 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디아미노피멜레이트 디카르복실라아제 (LysA, EC 4.1.1.20) 및 이를 코딩하는 폴리뉴클레오티드,
hhh) SEQ ID NO: 166 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 아미노트랜스페라아제 (AaT, EC 2.6.1.1) 및 이를 코딩하는 폴리뉴클레오티드,
iii) SEQ ID NO: 168 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 L-리신 익스포터 (exporter) (LysE, 리신 방출 (efflux) 퍼미아제) 및 이를 코딩하는 폴리뉴클레오티드,
jjj) SEQ ID NO: 170 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디히드로피콜리네이트 리덕타아제 (DapB, EC 1.3.1.26) 및 이를 코딩하는 폴리뉴클레오티드,
kkk) SEQ ID NO: 172 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 디히드로게나아제 (EC 1.1.1.49) 및 이를 코딩하는 폴리뉴클레오티드,
lll) SEQ ID NO: 186 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 디히드로게나아제의 Zwf 서브유닛 (Zwf, EC 1.1.1.49) 및 이를 코딩하는 폴리뉴클레오티드,
mmm) SEQ ID NO: 188 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 디히드로게나아제의 OpcA 서브유닛 (OpcA, EC 1.1.1.49) 및 이를 코딩하는 폴리뉴클레오티드,
nnn) SEQ ID NO: 174 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포글루콘산 디히드로게나아제 (Gnd, EC 1.1.1.44) 및 이를 코딩하는 폴리뉴클레오티드.
본 발명은 또한 추가로 상기 언급된 폴리뉴클레오티드를 포함하는 벡터 및 또한 효소 및/또는 폴리뉴클레오티드 및/또는 상기 언급된 벡터를 포함하는 재조합 미생물에 관한 것이다. 이러한 경우, 바람직한 구현예에서, 관련 폴리펩티드 및/또는 폴리뉴클레오티드는 과발현 형태로 미생물에 존재한다. 이러한 경우, 재조합 미생물은 바람직하게는 코리네형 박테리아, 특히 코리네박테리아, 특히 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아이다.
따라서, 본 발명은 또한 추가로 언급된 폴리뉴클레오티드 중 적어도 1 개, 바람직하게는 적어도 2, 3 또는 4 개가 과발현 형태로 존재하는 L-아미노산, 바람직하게는 L-알라닌, L-발린, 글루타메이트 패밀리의 L-아미노산, 특히 L-글루타메이트, L-글루타민, L-프롤린 및 L-아르기닌, 및 아스파르테이트 패밀리의 L-아미노산, 특히 L-아스파르테이트, L-아스파라긴, L-메티오닌, L-리신, L-이소류신 및 L-트레오닌으로부터 선택되는 L-아미노산, 특히 바람직하게는 L-알라닌, L-발린, L-글루타메이트, L-메티오닌, L-리신 및 L-트레오닌으로부터 선택되는 L-아미노산의 과잉생산 방법, 특히 L-메타오닌의 과잉생산 방법에 관한 것이며, 이때 상기 방법은 바람직하게는 코리네박테리아, 특히 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아에서 수행된다.
본 발명은 또한 추가로 C. 휴미레듀센스로부터의 다른 폴리뉴클레오티드 및 또한 상기 폴리뉴클레오티드에 의해 코딩된 폴리펩티드에 관한 것이다. 관련 폴리뉴클레오티드 또는 폴리펩티드의 불활성화 또는 감쇠에 의해, 특정 L-아미노산의 아미노산 생산은 긍정적인 영향을 받을 수 있다.
따라서, 본 발명은 또한 하기 목록으로부터 선택되는 폴리펩티드에 관한 것이다:
a) SEQ ID NO: 108 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트레오닌 신타아제 (ThrC, EC 4.2.3.1) 및 이를 코딩하는 폴리뉴클레오티드,
b) SEQ ID NO: 110 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소프로필말레이트 신타아제 (LeuA, EC 2.3.3.13) 및 이를 코딩하는 폴리뉴클레오티드,
c) SEQ ID NO: 112 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소프로필말레이트 디히드로게나아제 (LeuB, EC 1.1.1.85) 및 이를 코딩하는 폴리뉴클레오티드,
d) SEQ ID NO: 114 또는 SEQ ID NO: 116 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소프로필말레이트 이소메라아제의 서브유닛 (LeuCD, EC 4.2.1.33) 및 이를 코딩하는 폴리뉴클레오티드,
e) SEQ ID NO: 198 또는 SEQ ID NO: 200 에 따른 서열에 대해 각각, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 숙시닐-CoA 리가아제의 서브유닛 (SucCD, EC 6.2.1.5) 및 이를 코딩하는 폴리뉴클레오티드,
f) SEQ ID NO: 2 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 유형 HTH tetR 의 DNA 결합 도메인 (McbR) 및 이를 코딩하는 폴리뉴클레오티드,
g) SEQ ID NO: 4 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 키나아제 (ThrB, EC 2.7.1.39) 및 이를 코딩하는 폴리뉴클레오티드,
h) SEQ ID NO: 6 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 이소메라아제 (Pgi, EC 5.3.1.9) 및 이를 코딩하는 폴리뉴클레오티드,
i) SEQ ID NO: 8 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포에놀피루베이트 카르복시키나아제 (Pck, EC 4.1.1.32) 및 이를 코딩하는 폴리뉴클레오티드,
j) SEQ ID NO: 10 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 D-메티오닌-결합 지질단백질 (MetQ) 및 이를 코딩하는 폴리뉴클레오티드,
k) SEQ ID NO: 12 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 메티오닌 운반체 (MetP) 및 이를 코딩하는 폴리뉴클레오티드,
l) SEQ ID NO: 14 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 ATP-의존성 메티오닌 운반체 (MetN) 및 이를 코딩하는 폴리뉴클레오티드,
m) SEQ ID NO: 16 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 S-아데노실메티오닌 신타아제 (MetK) 및 이를 코딩하는 폴리뉴클레오티드,
n) SEQ ID NO: 18 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 메티오닌 도입 (import) 시스템 퍼미아제 (MetI) 및 이를 코딩하는 폴리뉴클레오티드,
o) SEQ ID NO: 20 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 4-히드록시-테트라히드로디피콜리네이트 신타아제 (DapA, EC 4.3.3.7) 및 이를 코딩하는 폴리뉴클레오티드,
p) SEQ ID NO: 24 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 카르복실레이트-아민 리가아제 및 이를 코딩하는 폴리뉴클레오티드,
q) SEQ ID NO: 176 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 말레이트:퀴논 옥시도리덕타아제 (Mqo, EC 1.1.99.16) 및 이를 코딩하는 폴리뉴클레오티드,
r) SEQ ID NO: 178 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 디히드로게나아제 복합체의 E1p 서브유닛 (AceE, EC 1.2.4.1) 및 이를 코딩하는 폴리뉴클레오티드,
s) SEQ ID NO: 180 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시트레이트 신타아제 (GltA, EC 4.1.3.7) 및 이를 코딩하는 폴리뉴클레오티드,
t) SEQ ID NO: 182 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 말레이트 디히드로게나아제 (Mdh, EC 1.1.1.37) 및 이를 코딩하는 폴리뉴클레오티드,
u) SEQ ID NO: 184 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 UDP-N-아세틸무라모일알라닐-D-글루타메이트-2,6-디아미노피멜레이트 리가아제 (MurE, EC 6.3.2.13) 및 이를 코딩하는 폴리뉴클레오티드.
본 발명은 또한 추가로 상기 언급된 폴리뉴클레오티드를 포함하는 벡터 및 또한 효소 및/또는 폴리뉴클레오티드 및/또는 상기 언급된 벡터를 포함하는 재조합 미생물에 관한 것이다. 이러한 경우, 바람직한 구현예에서, 관련 폴리펩티드 및/또는 폴리뉴클레오티드는 미생물에서 불활성화 또는 감쇠된 형태로 존재한다. 이러한 경우, 재조합 미생물은 바람직하게는 코리네형 박테리아, 특히 코리네박테리아, 특히 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아, 특히 C. 휴미레듀센스 종의 코리네박테리아이다.
따라서, 본 발명은 또한 추가로 언급한 폴리뉴클레오티드 중 적어도 1 개, 바람직하게는 적어도 2, 3, 또는 4 개가 불활성화 또는 감쇠된 형태로 존재하는 L-아미노산, 바람직하게는 L-알라닌, L-발린, 글루타메이트 패밀리의 L-아미노산, 특히 L-글루타메이트, L-글루타민, L-프롤린 및 L-아르기닌, 및 아스파르테이트 패밀리의 L-아미노산, 특히 L-아스파르테이트, L-아스파라긴, L-메티오닌, L-리신, L-이소류신 및 L-트레오닌으로부터 선택되는 L-아미노산, 특히 바람직하게는 L-알라닌, L-발린, L-글루타메이트, L-메티오닌, L-리신 및 L-트레오닌으로부터 선택되는 L-아미노산의 과잉생산 방법, 특히 L-메티오닌의 과잉 생산 밥법에 관한 것이며, 이때 상기 방법은 바람직하게는 코리네박테리아, 특히 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아에서 수행된다. 바람직한 구현예에서, 상기 상세한 목록에 언급한 폴리뉴클레오티드 중 적어도 1 개, 바람직하게는 적어도 2, 3, 또는 4 개가 동시에 과발현 형태로 존재한다.
본 발명에 따른 추가로 바람직한 구현예에서, 본 발명에 따른 미생물 또는 박테리아, 특히 본 발명에 따른 코리네박테리아, 특히 본 발명에 따른 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아, 특히 본 발명에 따른 L-메티오닌-과잉생산 균주, 본 발명에 추가로, 바람직하게는 과발현된 글리신 절단 시스템 또는 상기 시스템을 코딩하는 폴리뉴클레오티드는 하기 특징 중 적어도 1 개, 바람직하게는 적어도 2 또는 3 개, 특히 바람직하게는 적어도 4 또는 5 개를 갖는다:
a) HTH tetR 형의 DNA 결합 도메인 (McbR), 바람직하게는 SEQ ID NO: 2 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 DNA 결합 도메인를 코딩하는 감쇠된 폴리뉴클레오티드 (mcbR),
b) 호모세린 키나아제 (ThrB, EC 2.7.1.39), 바람직하게는 SEQ ID NO: 4 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 키나아제를 코딩하는 감쇠된 폴리뉴클레오티드 (thrB 유전자),
c) 글루코오스-6-포스페이트 이소메라아제 (Pgi, EC 5.3.1.9), 바람직하게는 SEQ ID NO: 6 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 이소메라아제를 코딩하는 감쇠된 폴리뉴클레오티드 (pgi),
d) 포스포에놀피루베이트 카르복시키나아제 (Pck, EC 4.1.1.32), 바람직하게는 SEQ ID NO: 8 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포에놀피루베이트 카르복시키나아제를 코딩하는 감쇠된 폴리뉴클레오티드 (pck),
e) D-메티오닌-결합 지질단백질 (MetQ), 바람직하게는 SEQ ID NO: 10 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 D-메티오닌-결합 지질단백질을 코딩하는 감쇠된 폴리뉴클레오티드 (metQ),
f) 메티오닌 운반체 (MetP), 바람직하게는 SEQ ID NO: 12 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 메티오닌 운반체를 코딩하는 감쇠된 폴리뉴클레오티드 (metP),
g) ATP-의존성 메티오닌 운반체 (MetN), 바람직하게는 SEQ ID NO: 14 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 ATP-의존성 메티오닌 운반체를 코딩하는 감쇠된 폴리뉴클레오티드 (metN),
h) S-아데노실메티오닌 신타아제 (MetK), 바람직하게는 SEQ ID NO: 16 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 S-아데노실메티오닌 신타아제를 코딩하는 감쇠된 폴리뉴클레오티드 (metK),
i) 메티오닌 도입 시스템 퍼미아제 (MetI), 바람직하게는 SEQ ID NO: 18 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 메티오닌 도입 시스템 퍼미아제를 코딩하는 감쇠된 폴리뉴클레오티드 (metI),
j) 4-히드록시-테트라히드로디피콜리네이트 신타아제 (DapA), 바람직하게는 SEQ ID NO: 20 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 4-히드록시-테트라히드로디피콜리네이트 신타아제를 코딩하는 감쇠된 폴리뉴클레오티드 (dapA),
k) 시스테인 신타아제 (CBS), 바람직하게는 SEQ ID NO: 22 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스테인 신타아제를 코딩하는 과발현 폴리뉴클레오티드 (CBS),
l) cg3031 동족체, 바람직하게는 SEQ ID NO: 24 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 cg3031 동족체를 코딩하는 감쇠된 폴리뉴클레오티드,
m) 시스타티오닌 베타-리아제 (AecD), 바람직하게는 SEQ ID NO: 26 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 베타-리아제를 코딩하는 과발현 폴리뉴클레오티드 (aecD),
n) 아스파르테이트 세미알데히드 디히드로게나아제 (Asd), 바람직하게는 SEQ ID NO: 28 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 세미알데히드 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (asd),
o) 5-메틸테트라히드로폴레이트 호모시스테인 메틸트랜스페라아제 (MetH, EC 2.1.1.13) 를 코딩하는 과발현 폴리뉴클레오티드 (metH),
p) 분지쇄 아미노산에 대한 운반체의 보다 작은 서브유닛 (BrnE), 바람직하게는 SEQ ID NO: 30 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (brnE),
q) 분지쇄 아미노산에 대한 운반체의 보다 큰 서브유닛 (BrnF), 바람직하게는 SEQ ID NO: 32 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (brnF),
r) 세린 아세틸트랜스페라아제 (CysE), 바람직하게는 SEQ ID NO: 34 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 세린 아세틸트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (cysE),
s) 시스테인 신타아제 (CysK), 바람직하게는 SEQ ID NO: 36 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스테인 신타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysK),
t) 세린 히드록시메틸트랜스페라아제 (GlyA), 바람직하게는 SEQ ID NO: 44 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 세린 히드록시메틸트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (glyA),
u) 임의적 피드백-내성 호모세린 디히드로게나아제 (Hom), 바람직하게는 SEQ ID NO: 46 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (hom),
v) 바람직하게는 피드백-내성 아스파르테이트 키나아제 (LysC), 바람직하게는 SEQ ID NO: 54 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 키나아제를 코딩하는 과발현 폴리뉴클레오티드 (lysC),
w) 시스타티오닌 감마-신타아제 (MetB), 바람직하게는 SEQ ID NO: 56 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 감마-신타아제를 코딩하는 과발현 폴리뉴클레오티드 (metB),
x) 5,10-메틸렌테트라히드로폴레이트 리덕타아제 (MetF), 바람직하게는 SEQ ID NO: 58 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 5,10-메틸렌테트라히드로폴레이트 리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (metF),
y) 호모세린 O-아세틸트랜스페라아제 (MetX), 바람직하게는 SEQ ID NO: 60 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 O-아세틸트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (metX),
z) O-아세틸호모세린 리아제 (MetY), 바람직하게는 SEQ ID NO: 62 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 O-아세틸호모세린 리아제를 코딩하는 과발현 폴리뉴클레오티드 (metY),
aa) 피루베이트 카르복실라아제 (Pyc), 바람직하게는 SEQ ID NO: 64 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 카르복실라아제를 코딩하는 과발현 폴리뉴클레오티드 (pyc),
bb) 임의적 피드백-내성 D-3-포스포글리세레이트 디히드로게나아제 (SerA), 바람직하게는 SEQ ID NO: 66 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 D-3-포스포글리세레이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (serA),
cc) 포스포세린 포스파타아제 (SerB), 바람직하게는 SEQ ID NO: 68 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포세린 포스파타아제를 코딩하는 과발현 폴리뉴클레오티드 (serB),
dd) 포스포세린 아미노트랜스페라아제 (SerC), 바람직하게는 SEQ ID NO: 70 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포세린 아미노트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (serC),
ee) 알라닌 디히드로게나아제 (Ald), 바람직하게는 SEQ ID NO: 72 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 알라닌 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (ald),
ff) 술페이트 아데닐릴트랜스페라아제의 서브유닛 (CysD), 바람직하게는 SEQ ID NO: 74 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (cysD),
gg) 아데노신 포스포술페이트 리덕타아제 (CysH), 바람직하게는 SEQ ID NO: 76 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아데노신 포스포술페이트 리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysH),
hh) 술파이트 리덕타아제 (CysI), 바람직하게는 SEQ ID NO: 78 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술파이트 리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysI),
ii) (CysJ), 바람직하게는 SEQ ID NO: 80 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 것을 코딩하는 과발현 폴리뉴클레오티드 (cysJ),
jj) 술페이트 아데닐릴트랜스페라아제의 서브유닛 (CysN), 바람직하게는 SEQ ID NO: 82 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (cysN),
kk) 시스타티오닌 베타-신타아제 (CysY), 바람직하게는 SEQ ID NO: 84 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 베타-신타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysY),
ll) 추정 상의 (putative) 술페이트 운반체 (CysZ), 바람직하게는 SEQ ID NO: 86 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술페이트 운반체를 코딩하는 과발현 폴리뉴클레오티드 (cysZ),
mm) 5-메틸테트라히드로프테로일트리글루타메이트-호모시스테인 메틸트랜스페라아제 (MetE), 바람직하게는 SEQ ID NO: 88 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 단백질을 코딩하는 과발현 폴리뉴클레오티드 (metE),
nn) 펩티딜-tRNA 히드롤라아제 1 (PtH1), 바람직하게는 SEQ ID NO: 90 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 펩티딜-tRNA 히드롤라아제 1 을 코딩하는 과발현 폴리뉴클레오티드 (ptH1),
oo) 펩티딜-tRNA 히드롤라아제 2 (PtH2), 바람직하게는 SEQ ID NO: 92 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 펩티딜-tRNA 히드롤라아제 2 를 코딩하는 과발현 폴리뉴클레오티드 (ptH2).
본 발명은 또한 추가로 이와 같은 미생물 또는 박테리아를 사용하는 L-아미노산, 특히 L-메티오닌의 상응하는 과잉생산 방법에 관한 것이다.
본 발명에 따른 추가로 바람직한 구현예에서, 본 발명에 따른 미생물 또는 박테리아, 특히 본 발명에 따른 코리네박테리아, 특히 본 발명에 따른 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아, 특히 본 발명에 따른 L-발린-과잉생산 균주, 본 발명에 추가로, 바람직하게는 과발현된 글리신 절단 시스템 또는 상기 시스템을 코딩하는 폴리뉴클레오티드는 하기 특징 중 적어도 1 개, 바람직하게는 적어도 2 또는 3 개, 특히 바람직하게는 적어도 4 또는 5 개를 갖는다:
a) 트레오닌 디히드라타아제 (IlvA EC 4.3.1.19), 바람직하게는 SEQ ID NO: 106 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트레오닌 디히드라타아제를 코딩하는 과발현 폴리뉴클레오티드 (ilvA 유전자),
b) 아세토락테이트 신타아제의 서브유닛 (IlvB), 바람직하게는 SEQ ID NO: 98 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아세토락테이트 신타아제의 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (ilvB 유전자),
c) 아세토락테이트 신타아제의 임의적 피드백-내성 서브유닛 (IlvN) 을 코딩하는 과발현 폴리뉴클레오티드 (ilvN 유전자),
d) 이소메로리덕타아제 (IlvC, EC 1.1.1.86), 바람직하게는 SEQ ID NO: 100 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소메로리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (ilvC 유전자),
e) 디히드록시산 디히드라타아제 (IlvD, EC 4.2.1.9), 바람직하게는 SEQ ID NO: 102 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디히드록시산 디히드라타아제를 코딩하는 과발현 폴리뉴클레오티드 (ilvD 유전자),
f) 트랜스아미나아제 (IlvE, EC 2.6.1.42), 바람직하게는 SEQ ID NO: 104 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트랜스아미나아제를 코딩하는 과발현 폴리뉴클레오티드 (ilvE 유전자),
g) 아세토락테이트 신타아제 (IlvH, EC 2.2.1.6), 바람직하게는 SEQ ID NO: 122 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아세토락테이트 신타아제를 코딩하는 과발현 폴리뉴클레오티드 (ilvH 유전자),
h) 호모세린 키나아제 (ThrB, EC 2.7.1.39), 바람직하게는 SEQ ID NO: 4 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 키나아제를 코딩하는 감쇠된 폴리뉴클레오티드 (thrB 유전자),
i) 트레오닌 신타아제 (ThrC, EC 4.2.3.1), 바람직하게는 SEQ ID NO: 108 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트레오닌 신타아제를 코딩하는 감쇠된 폴리뉴클레오티드 (thrC 유전자),
j) 임의적 피드백-내성 호모세린 디히드로게나아제 (Hom, EC 1.2.1.11), 바람직하게는 SEQ ID NO: 46 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (hom 유전자),
k) 임의적 피드백-내성 이소프로필말레이트 신타아제 (LeuA, EC 2.3.3.13), 바람직하게는 SEQ ID NO: 110 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소프로필말레이트 신타아제를 코딩하는 감쇠된 폴리뉴클레오티드 (leuA 유전자),
l) 이소프로필말레이트 디히드로게나아제 (LeuB, EC 1.1.1.85), 바람직하게는 SEQ ID NO: 112 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소프로필말레이트 디히드로게나아제를 코딩하는 감쇠된 폴리뉴클레오티드 (leuB 유전자),
m) 이소프로필말레이트 이소메라아제의 서브유닛 (LeuCD, EC 4.2.1.33), 바람직하게는 SEQ ID NO: 114 및 SEQ ID NO: 116 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소프로필말레이트 이소메라아제의 서브유닛을 코딩하는 감쇠된 폴리뉴클레오티드 (leuCD 유전자),
n) 3-메틸-2-옥소부타노에이트 히드록시메틸트랜스페라아제 (PanB, EC 2.1.2.11), 바람직하게는 SEQ ID NO: 118 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 3-메틸-2-옥소부타노에이트 히드록시메틸트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (panB 유전자),
o) 판토테네이트 신타아제 (PanC, EC 6.3.2.1), 바람직하게는 SEQ ID NO: 120 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 판토테네이트 신타아제를 코딩하는 과발현 폴리뉴클레오티드 (panC 유전자).
따라서, 본 발명은 또한 추가로 이와 같은 미생물 또는 박테리아를 사용하는 L-아미노산, 특히 L-발린의 과잉생산 방법에 관한 것이다.
본 발명에 따른 추가로 바람직한 구현예에서, 본 발명에 따른 미생물 또는 박테리아, 특히 본 발명에 따른 코리네박테리아, 특히 본 발명에 따른 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아, 특히 본 발명에 따른 L-글루타메이트-과잉생산 균주, 본 발명에 추가로, 바람직하게는 과발현 글리신 절단 시스템 또는 상기 시스템을 코딩하는 폴리뉴클레오티드는 하기 특징 중 적어도 1 개, 바람직하게는 적어도 2 또는 3 개, 특히 바람직하게는 적어도 4 또는 5 개를 갖는다:
a) 글루타메이트 디히드로게나아제 (Gdh), 바람직하게는 SEQ ID NO: 124 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타메이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (gdh),
b) 글루타민 신테타아제 (글루타민 신테타아제 1), 바람직하게는 SEQ ID NO: 126 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타민 신테타아제를 코딩하는 과발현 폴리뉴클레오티드,
c) 글루타민 신테타아제 (글루타민 신테타아제 2), 바람직하게는 SEQ ID NO: 128 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타민 신테타아제를 코딩하는 과발현 폴리뉴클레오티드,
d) 글루타메이트 신타아제, 바람직하게는 SEQ ID NO: 130 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루타메이트 신타아제를 코딩하는 과발현 폴리뉴클레오티드,
e) 이소시트레이트 디히드로게나아제, 바람직하게는 SEQ ID NO: 132 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 이소시트레이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드,
f) 아코니테이트 히드라아제, 바람직하게는 SEQ ID NO: 134 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아코니테이트 히드라아제를 코딩하는 과발현 폴리뉴클레오티드,
g) 시트레이트 신타아제, 바람직하게는 SEQ ID NO: 136 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시트레이트 신타아제를 코딩하는 과발현 폴리뉴클레오티드,
h) 아미노펩티다아제 C (PepC), 바람직하게는 SEQ ID NO: 138 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아미노펩티다아제 C 를 코딩하는 과발현 폴리뉴클레오티드 (pepC),
i) 피루베이트 디히드로게나아제, 바람직하게는 SEQ ID NO: 140 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드,
j) 피루베이트 키나아제 (피루베이트 키나아제 1), 바람직하게는 SEQ ID NO: 142 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 키나아제를 코딩하는 과발현 폴리뉴클레오티드,
k) 피루베이트 키나아제 (피루베이트 키나아제 2), 바람직하게는 SEQ ID NO: 144 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 키나아제를 코딩하는 과발현 폴리뉴클레오티드,
l) 에놀라아제, 바람직하게는 SEQ ID NO: 146 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 에놀라아제를 코딩하는 과발현 폴리뉴클레오티드,
m) 2,3-비스포스포글리세레이트-의존성 포스포글리세레이트 뮤타아제 (GpmA), 바람직하게는 SEQ ID NO: 148 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포글리세레이트 뮤타아제를 코딩하는 과발현 폴리뉴클레오티드 (gpmA),
n) 포스포글리세레이트 키나아제 (Pgk), 바람직하게는 SEQ ID NO: 150 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포글리세레이트 키나아제를 코딩하는 과발현 폴리뉴클레오티드 (pgk),
o) 글리세르알데히드-3-포스페이트 디히드로게나아제 (글리세롤-3-포스페이트 디히드로게나아제 1), 바람직하게는 SEQ ID NO: 152 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글리세르알데히드-3-포스페이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드,
p) 글리세르알데히드-3-포스페이트 디히드로게나아제 (글리세롤-3-포스페이트 디히드로게나아제 2), 바람직하게는 SEQ ID NO: 154 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글리세르알데히드-3-포스페이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드,
q) 트리오스포스페이트 이소메라아제 (TpiA), 바람직하게는 SEQ ID NO: 156 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 트리오스포스페이트 이소메라아제를 코딩하는 과발현 폴리뉴클레오티드 (tpiA),
r) 프럭토오스 비스포스페이트 알돌라아제, 바람직하게는 SEQ ID NO: 158 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 프럭토오스 비스포스페이트 알돌라아제를 코딩하는 과발현 폴리뉴클레오티드,
s) 1-포스포프럭토키나아제, 바람직하게는 SEQ ID NO: 160 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 1-포스포프럭토키나아제를 코딩하는 과발현 폴리뉴클레오티드,
t) 6-포스포프럭토키나아제, 바람직하게는 SEQ ID NO: 162 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 6-포스포프럭토키나아제를 코딩하는 과발현 폴리뉴클레오티드,
u) 글루코오스-6-포스페이트 이소메라아제, 바람직하게는 SEQ ID NO: 6 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 이소메라아제를 코딩하는 과발현 폴리뉴클레오티드 (pgi),
v) 숙시닐-CoA 리가아제의 서브유닛 (SucCD, EC 6.2.1.5), 바람직하게는 SEQ ID NO: 198 또는 SEQ ID NO: 200 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 숙시닐-CoA 리가아제의 서브유닛을 코딩하는 감쇠된 폴리뉴클레오티드 (sucCD).
따라서, 본 발명은 또한 추가로 이와 같은 미생물 또는 박테리아를 사용하는, L-아미노산, 특히 L-글루타메이트의 과잉생산 방법에 관한 것이다.
본 발명에 따른 추가로 바람직한 구현예에서, 본 발명에 따른 미생물 또는 박테리아, 특히 본 발명에 따른 코리네박테리아, 특히 본 발명에 따른 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아, 특히 본 발명에 따른 L-알라닌-과잉생산 균주, 본 발명에 추가로, 바람직하게는 과발현 글리신 절단 시스템 또는 상기 시스템을 코딩하는 폴리뉴클레오티드는 하기 특징 중 적어도 1 개, 바람직하게는 적어도 2 또는 3 개, 특히 바람직하게는 적어도 4 또는 5 개를 갖는다:
a) 알라닌 디히드로게나아제 (AlaD), 바람직하게는 코리네박테리아로부터의 알라닌 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (alaD),
b) 글리세르알데히드-3-포스페이트 디히드로게나아제 (GapA), 바람직하게는 코리네박테리아로부터의 글리세르알데히드-3-포스페이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (gapA),
c) L-락테이트 디히드로게나아제 (LdhA), 바람직하게는 코리네박테리아로부터의 L-락테이트 디히드로게나아제를 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (ldhA),
d) 포스포에놀피루베이트 카르복실라아제 (Ppc), 바람직하게는 코리네박테리아로부터의 포스포에놀피루베이트 카르복실라아제를 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (ppc),
e) 알라닌 라세마아제 (Alr), 바람직하게는 코리네박테리아로부터의 알라닌 라세마아제를 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (alr).
따라서, 본 발명은 또한 추가로 이와 같은 미생물 또는 박테리아를 사용하는, L-아미노산, 특히 L-알라닌의 과잉생산 방법에 관한 것이다.
본 발명에 따른 추가로 바람직한 구현예에서, 본 발명에 따른 미생물 또는 박테리아, 특히 본 발명에 따른 코리네박테리아, 특히 본 발명에 따른 C. 휴미레듀센스 또는 C. 글루타미쿰 종의 코리네박테리아, 특히 본 발명에 따른 L-리신-과잉생산 균주, 본 발명에 추가로, 바람직하게는 과발현된 글리신 절단 시스템 또는 상기 시스템을 코딩하는 폴리뉴클레오티드는 하기 특징 중 적어도 1 개, 바람직하게는 적어도 2 또는 3 개, 특히 바람직하게는 적어도 4 또는 5 개를 갖는다:
a) 디히드로디피콜리네이트 신타아제 (DapA, EC 4.2.1.52), 바람직하게는 SEQ ID NO: 20 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디히드로디피콜리네이트 신타아제를 코딩하는 과발현 폴리뉴클레오티드 (dapA 유전자),
b) 바람직하게는 피드백-내성 아스파르테이트 키나아제 (LysC, EC 2.7.2.4), 바람직하게는 SEQ ID NO: 54 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 키나아제를 코딩하는 과발현 폴리뉴클레오티드 (lysC),
c) 디아미노피멜레이트 디히드로게나아제 (Ddh, EC 1.4.1.16), 바람직하게는 SEQ ID NO: 202 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디아미노피멜레이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (ddh),
d) 아스파르테이트 세미알데히드 디히드로게나아제 (Asd, EC 1.2.1.11), 바람직하게는 SEQ ID NO: 28 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 세미알데히드 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (asd 유전자),
e) 디아미노피멜레이트 디카르복실라아제 (LysA, EC 4.1.1.20), 바람직하게는 SEQ ID NO: 164 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디아미노피멜레이트 디카르복실라아제를 코딩하는 과발현 폴리뉴클레오티드 (lysA 유전자),
f) 아스파르테이트 아미노트랜스페라아제 (AaT, EC 2.6.1.1), 바람직하게는 SEQ ID NO: 166 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 아미노트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (aat 유전자),
g) L-리신 익스포터 (LysE, 리신 방출 퍼미아제), 바람직하게는 SEQ ID NO: 168 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 L-리신 익스포터를 코딩하는 과발현 폴리뉴클레오티드 (lysE 유전자),
h) 피루베이트 카르복실라아제 (Pyc, EC 6.4.1.1), 바람직하게는 SEQ ID NO: 64 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 카르복실라아제를 코딩하는 과발현 폴리뉴클레오티드 (pyc 유전자),
i) 디아미노피멜레이트 에피메라아제 (DapF, EC 5.1.1.7) 를 코딩하는 과발현 폴리뉴클레오티드 (dapF 유전자),
j) 디히드로피콜리네이트 리덕타아제 (DapB, EC 1.3.1.26), 바람직하게는 SEQ ID NO: 172 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 디히드로피콜리네이트 리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (dapB 유전자),
k) 글루코오스-6-포스페이트 디히드로게나아제 (EC 1.1.1.49), 바람직하게는 SEQ ID NO: 174 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드,
l) 글루코오스-6-포스페이트 디히드로게나아제의 Zwf 서브유닛 (Zwf, EC 1.1.1.49), 바람직하게는 SEQ ID NO: 188 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 Zwf 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (zwf 유전자),
m) 글루코오스-6-포스페이트 디히드로게나아제의 OpcA 서브유닛 (OpcA, EC 1.1.1.49), 바람직하게는 SEQ ID NO: 190 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 OpcA 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (opcA 유전자),
n) 포스포글루콘산 디히드로게나아제 (Gnd, EC 1.1.1.44), 바람직하게는 SEQ ID NO: 176 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포글루콘산 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (gnd 유전자),
o) 말레이트:퀴논 옥시도리덕타아제 (Mqo, EC 1.1.99.16), 바람직하게는 SEQ ID NO: 178 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 말레이트:퀴논 옥시도리덕타아제를 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (mqo),
p) 피루베이트 디히드로게나아제 복합체의 E1p 서브유닛 (AceE, EC 1.2.4.1), 바람직하게는 SEQ ID NO: 180 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 E1p 서브유닛을 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (aceE),
q) 시트레이트 신타아제 (GltA, EC 4.1.3.7), 바람직하게는 SEQ ID NO: 182 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시트레이트 신타아제를 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (gltA),
r) 말레이트 디히드로게나아제 (Mdh, EC 1.1.1.37), 바람직하게는 SEQ ID NO: 184 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 말레이트 디히드로게나아제를 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (mdh),
s) UDP-N-아세틸무라모일알라닐-D-글루타메이트-2,6-디아미노피멜레이트 리가아제 (MurE, EC 6.3.2.13), 바람직하게는 SEQ ID NO: 186 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 효소를 코딩하는 불활성화 또는 감쇠된 폴리뉴클레오티드 (murE).
따라서, 본 발명은 또한 추가로 이와 같은 미생물 또는 박테리아를 사용하는, L-아미노산, 특히 L-리신의 과잉생산 방법에 관한 것이다.
상기 언급된 본 발명에 따른 방법에서 사용되거나 사용하고자 하는 폴리뉴클레오티드 및 폴리펩티드는, 바람직하게는 코리네박테리아, 특히 C. 글루타미쿰 또는 C. 휴미레듀센스, 특히 바람직하게는 C. 휴미레듀센스에서 유래된다.
본 발명에 따른 "과발현" 은, 개시 균주 (모균주) 또는 야생형 균주에 비해, 일반적으로 미생물 내에서 해당 DNA 에 의해 코딩되는 리보핵산, 단백질 (폴리펩티드) 또는 효소의 세포내 농도 또는 활성의 증가를 의미하는 것으로 이해된다. 개시 균주 (모균주) 는 과발현을 유도하는 조치가 취해진 균주를 의미한다.
농도 또는 활성의 증가는, 예를 들어 염색체 또는 염색체 외로, 적어도 하나의 복제에 의해, 해당 코딩 폴리뉴클레오티드의 복제 수를 증가시킴으로써 달성될 수 있다.
복제 수를 증가시키는 보편적 방법은, 해당 코딩 폴리뉴클레오티드를 미생물, 특히 코리네형 박테리아로부터 복제된 벡터, 바람직하게는 플라스미드 내로 혼입하는 것으로 이루어진다. 또한, 전이인자 (transposon), 삽입 요소 (IS 요소) 또는 파지 (phage) 가 벡터로서 사용될 수 있다. 다수의 적합한 벡터가 선행 기술에 기재되어 있다.
과발현을 달성하기 위한 또 다른 보편적 방법은, 염색체 유전자 증폭 방법이다. 이러한 방법에서, 주목되는 폴리뉴클레오티드의 적어도 하나의 부가적인 복제는 코리네형 박테리아의 염색체 내에 삽입된다. 상기 증폭 방법은, 예를 들어 WO 03/014330 또는 WO 03/040373 에 기재되어 있다.
과발현을 달성하기 위한 추가의 방법은, 프로모터 또는 발현 카세트에 기능적 방식 (조작가능하게 연결됨) 으로 해당 유전자 또는 대립유전자를 연결하는 것으로 이루어진다. 코리네박테리움 글루타미쿰에 대한 적합한 프로모터가, 예를 들어 Patek 등. (Journal of Biotechnology 104(1-3), 311-323 (2003)) 의 문헌의 도 1 및 포괄 문헌, 예컨대 "Handbook of Corynebacterium glutamicum" (Eds.: Lothar Eggeling and Michael Bott, CRC Press, Boca Raton, US (2005)) 또는 서적 "Corynebacteria, Genomics and Molecular Biology" (Ed.: Andreas Burkovski, Caister Academic Press, Norfolk, UK (2008)) 에 기재되어 있다. 동일한 방식으로, 예를 들어 Vasicova 등 (Journal 의 Bacteriology 181, 6188-6191 (1999)) 에 기재되어 있는 dapA 프로모터, 프로모터 A25 의 변주가 사용될 수 있다. 또한, 코리네박테리움 글루타미쿰의 gap 프로모터가 사용될 수 있다 (EP 06007373). 최종적으로, Amann 등. (Gene 69(2), 301-315 (1988)) 및 Amann and Brosius (Gene 40(2-3), 183-190 (1985)) 에 의해 기재된 잘 알려진 프로모터 T3, T7, SP6, M13, lac, tac 및 trc 가 사용될 수 있다. 이와 같은 프로모터는, 예를 들어 관련 유전자의 업스트림, 통상적으로 개시 코돈으로부터 약 1-500 핵염기의 거리에서 삽입될 수 있다.
과발현의 조치는 해당 폴리펩티드의 활성 또는 농도를, 과발현에서 수득되는 조치 전에 균주 중 상기 폴리펩티드의 활성 또는 농도를 기준으로, 바람직하게는 10%, 25%, 50%, 75%, 100%, 150%, 200%, 300%, 400% 또는 500% 이상, 바람직하게는 1000% 또는 2000% 이하로 증가시킨다.
단백질의 농도는 1- 및 2-차원 단백질 겔 분리 및 겔 중 적절한 평가 소프트웨어로의 단백질 농도의 후속 광학적 식별을 통해 측정될 수 있다. 코리네형 박테리아용 단백질 겔의 생산 및 상기 단백질을 식별하는 통상적인 방법은, Hermann 등. (Electrophoresis, 22:1712-23 (2001)) 에 의해 기재된 절차이다. 마찬가지로, 단백질 농도는 검출되는 단백질에 대해 특이적인 항체를 사용한, 웨스턴 블롯 혼성 (Western blot hybridization) (Sambrook 등., Molecular Cloning: a laboratory manual, 2nd Ed. 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989) 및 농도 측정용 해당 소프트웨어를 사용한 후속 광학적 평가 (Lohaus and Meyer (1998) Biospektrum 5:32-39; Lottspeich, Angewandte Chemie 38: 2630-2647 (1999)) 에 의해 측정될 수 있다. 활성은 적합한 효소 검정을 이용하여 측정될 수 있다.
본 발명에 따른 "감쇠" 는, 개시 균주 (모균주) 또는 야생형 균주에 비해, 미생물 중 해당 DNA 에 의해 코딩되는 리보핵산, 단백질 (폴리펩티드) 또는 효소의 세포내 농도 또는 활성이 감소하는 것을 의미한다. 개시 균주 (모균주) 는 감쇠에 대한 조치가 취해진 균주를 의미한다.
감쇠는 폴리펩티드의 발현을 감소시킴으로써, 예를 들어 약한 프로모터를 사용함으로써 또는 보다 낮은 활성을 갖는 폴리펩티드를 코딩하는 대립유전자를 사용함으로써 달성될 수 있고, 임의적으로 이러한 조치는 조합될 수 있다. 감쇠는 또한, 예를 들어 코딩 유전자를 불활성화시킴으로써, 폴리펩티드의 발현을 완전히 방지함으로써 달성될 수 있다.
감쇠의 조치는, 해당 폴리펩티드의 활성 또는 농도를, 감쇠에서 수득되는 조치 전에 균주 중 상기 폴리펩티드의 활성 또는 농도를 기준으로, 바람직하게는 10%, 25%, 50% 또는 75% 이상, 100% 이하로 감소시킨다. 바람직한 구현예에서, 감쇠는 관련 폴리펩티드의 발현을 완전히 불활성화시키는 것으로 이루어진다.
아미노산 생산과 관련된 피드백-내성 효소는, 일반적으로 야생형에 비해 L-아미노산 및/또는 생산된 이의 유사체에 의한 저해에 대해 보다 낮은 민감성을 갖는 효소를 의미하는 것으로 이해된다.
특히, 피드백-내성 아스파르테이트 키나아제 (LysCFBR) 는, 야생형에 비해 리신 및 트레오닌의 혼합물 또는 AEC (아미노에틸시스테인) 및 트레오닌의 혼합물 또는 단독의 리신 또는 단독의 AEC 에 의한 저해에 대해 보다 낮은 민감성을 보이는 아스파르테이트 키나아제를 의미한다. 리신 생산을 위해, 바람직하게는 상기 피드백-내성 또는 탈감작화된 아스파르테이트 키나아제를 포함하는 해당 균주가 사용된다.
예를 들어, C. 글루타미쿰으로부터의 하기 피드백-내성 아스파르테이트 키나아제가 문헌으로부터 알려져 있다: A279T, A279V, S301F, S301Y, T308I, T311I, R320G, G345D, S381F. C. 글루타미쿰으로부터의 피드백-내성 아스파르테이트 키나아제에 관해, 하기 출원이 또한 참조된다: JP1993184366-A, JP1994062866-A, JP1994261766-A, JP1997070291-A, JP1997322774-A, JP1998165180-A, JP1998215883-A, US5688671-A, EP0387527, WO00/63388, US3732144, JP6261766, Jetten 등. (1995; Applied Microbiology Biotechnology 43: 76-82). C. 글루타미쿰으로부터의 피드백-내성 아스파르테이트 키나아제는 하기 수탁 번호로 NCBI GenBank 에 수탁되어 있다: E05108, E06825, E06826, E06827, E08177, E08178, E08179, E08180, E08181, E08182, E12770, E14514, E16352, E16745, E16746, I74588, I74589, I74590, I74591, I74592, I74593, I74594, I74595, I74596, I74597, X57226, L16848, L27125.
본 발명에 따른 C. 휴미레듀센스로부터의 하기 피드백-내성 아스파르테이트 키나아제가 바람직하게는 사용된다: D274Y, A279E, S301Y, T308I, T311I, G359D.
트레오닌 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 호모세린 디히드로게나아제 (HomFBR) 를 포함하는 균주를 사용하는 것이다.
이소류신 생산 및 발린 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 아세토락테이트 신타아제를 포함하는 균주를 사용하는 것이다.
류신 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 이소프로필말레이트 신타아제 (LeuAFBR) 를 포함하는 균주를 사용하는 것이다.
프롤린 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 글루타메이트-5-키나아제 (ProBFBR) 를 포함하는 균주를 사용하는 것이다.
아르기닌 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 오르니틴 카르바모일트랜스페라아제 (ArgFFBR) 를 포함하는 균주를 사용하는 것이다.
세린 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 D-3-포스포글리세레이트 디히드로게나아제 (SerAFBR) 를 포함하는 균주를 사용하는 것이다.
메티오닌 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 D-3-포스포글리세레이트 디히드로게나아제 (SerAFBR) 및/또는 피드백-내성 피루베이트 카르복실라아제 (PycFBR) 를 포함하는 균주를 사용하는 것이다.
트립토판 생산을 위해, 마찬가지로 바람직한 것은 해당 피드백-내성 포스포-2-디히드로-3-디옥시헵토네이트 알돌라아제 (AroGFBR 또는 AroHFBR) 를 포함하는 균주를 사용하는 것이다.
본 발명에 따라 사용되는 L-아미노산 과잉생산 C. 휴미레듀센스 균주의 보다 바람직한 추가 특성에 관해, 상기 인용된 Wu 등. (2011) 의 문헌 및 상기 언급된 기타 문헌이 참조된다.
본 발명에 따른 미생물, 특히 코리네박테리움 속의 박테리아는 L-리신을 생산하기 위한 회분식 조작 (회분식 배양 또는 회분식 방법) 또는 유가 배양 (fed batch) 또는 반복적 유가 배양 조작에서 연속적으로 - 예를 들어, WO 05/021772 에 기재된 바와 같이 - 또는 불연속적으로 배양될 수 있다. 알려진 배양 방법의 일반적인 개괄이, Chmiel (Bioprozesstechnik 1. Einfuhrung in die Bioverfahrenstechnik [Bioprocess Technology 1. Introduction to Bioprocess Technology] (Gustav Fischer Verlag, Stuttgart, 1991)) 에 의한 교과서 또는 Storhas (Bioreaktoren und periphere Einrichtungen [Bioreactors and Peripheral Devices] (Vieweg Verlag, Braunschweig/ Wiesbaden, 1994)) 에 의한 교과서에서 확인가능하다.
사용되는 배양 배지 또는 발효 배지는 적합한 방식으로 특정 균주의 요구를 충족시켜야 한다. 상이한 미생물의 배양 배지가, 미국 세균 학회 (American Society for Bacteriology, (Washington D.C., USA, 1981)) 의 소책자 "Manual of Methods for General Bacteriology" 에 설명되어 있다. 용어 배양 배지 및 발효 배지 또는 배지는 상호 교환가능하다.
사용되는 탄소 공급원은 당 및 탄수화물, 예컨대 글루코오스, 수크로오스, 락토오스, 프럭토오스, 말토오스, 당밀 (molasses), 사탕무 또는 사탕수수 제조로부터의 수크로오스-함유 용액, 녹말, 녹말 가수분해물 및 셀룰로오스, 오일 및 유지, 예컨대 대두 오일, 해바라기 오일, 땅콩 오일 및 코코넛 유지, 지방산, 예컨대 팔미트산, 스테아르산 및 리놀레산, 알코올, 예컨대 글리세롤, 메탄올 및 에탄올 및 유기산, 예컨대 아세트산 또는 락트산일 수 있다.
질소 공급원으로서, 유기 질소-함유 화합물, 예컨대 펩톤, 효모 추출물, 육류 추출물, 맥아 추출물, 옥수수 침지액, 대두분 및 요소, 또는 무기 화합물, 예컨대 암모늄 술페이트, 암모늄 클로라이드, 암모늄 포스페이트, 암모늄 카르보네이트 및 암모늄 니트레이트를 사용할 수 있다. 질소 공급원은 개별적으로 또는 혼합물로서 사용될 수 있다.
사용되는 인 공급원은 인산, 인산이수소칼륨 또는 인산수소칼륨 또는 해당 나트륨-함유 염일 수 있다.
배양 배지는, 성장을 위해 요구되는, 예를 들어 금속, 예컨대 나트륨, 칼륨, 마그네슘, 칼슘 및 철의 클로라이드 또는 술페이트 형태의 염, 예를 들어 마그네슘 술페이트 또는 철 술페이트의 염을 부가적으로 함유해야 한다. 최종적으로, 상기 언급된 물질에 더불어 필수 성장 인자, 예컨대 아미노산, 예를 들어 호모세린 및 비타민, 예를 들어 티아민, 비오틴 또는 판토텐산이 사용될 수 있다.
언급된 공급 원료는 단일 혼합물의 형태로 배양물에 첨가될 수 있거나 적절한 방식으로 배양 중 공급될 수 있다.
배양물의 pH 는 염기성 화합물, 예컨대 나트륨 히드록시드, 칼륨 히드록시드, 암모니아 또는 수성 암모니아, 또는 산성 화합물, 예컨대 인산 또는 황산을 적합한 방식으로 이용함으로써 제어될 수 있다. pH 는, 일반적으로 6.0 내지 9.0, 바람직하게는 6.5 내지 8 의 값으로 조정된다. 발포체의 진전을 제어하기 위해, 소포제, 예를 들어 지방산 폴리글리콜 에스테르를 사용할 수 있다. 플라스미드의 안정성을 유지하기 위해, 예를 들어 항생제와 같은 적합한 선택적 물질을 배지에 첨가할 수 있다. 호기성 조건을 유지하기 위해, 산소 또는 산소 기체 혼합물, 예를 들어 공기가 배양물 내로 도입된다. 마찬가지로, 과산화수소가 풍부한 액체의 사용이 가능하다. 적절한 경우, 발효는 승압, 예를 들어 0.03 내지 0.2 MPa 의 압력에서 실시된다. 배양물의 온도는 통상적으로 20℃ 내지 45℃ 및 바람직하게는 25℃ 내지 40℃ 이다. 회분식 조작에서, 배양은 목적하는 L-아미노산의 최대치가 형성될 때까지 계속된다. 이러한 목적은 통상적으로 10 시간 내지 160 시간 내에 달성된다. 연속적인 조작에서는, 배양 시간이 보다 길 수 있다. 박테리아의 활성은 발효 배지 및/또는 박테리아 세포 중 L-아미노산의 농축 (축적) 을 유도한다.
적합한 발효 배지의 예는, 그 중에서도, 특허 출원 5,770,409, US 5,840,551 및 US 5,990,350 또는 US 5,275,940 에서 확인된다.
발효 중, 하나 이상의 시간(들)에서의 농도를 측정하기 위한 L-아미노산의 분석은, Spackman 등. (Analytical Chemistry 30 : 1190-1206 (1958)) 에 기재된 바와 같이, 닌히드린 (ninhydrin) 을 이용하여 후속 용리후 유도체화 (post-column derivatization) 하면서, 이온 교환 크로마토그래피, 바람직하게는 양이온 교환 크로마토그래피로 L-아미노산을 분리함으로써 수행될 수 있다. 또한, 용리후 유도체화에서 닌히드린보다 오르토-프탈알데히드를 이용할 수 있다. 이온 교환 크로마토그래피에 대한 개요 문헌은, Pickering (LC·GC (Magazine of Chromatographic Science) 7(6), 484-487 (1989)) 에서 확인될 수 있다.
마찬가지로, 예를 들어 오르토-프탈알데히드 또는 페닐 이소티오시아네이트를 사용하는 용리전 유도체화 (pre-column derivatization) 을 수행하고, 역상 크로마토그래피 (RP), 바람직하게는 고압 액체 크로마토그래피 (HPLC) 의 형태로 수득한 아미노산을 분리할 수 있다. 이러한 유형의 방법은, 예를 들어 Lindroth 등. (Analytical Chemistry 51: 1167 - 1174 (1979)) 에 기재되어 있다.
검출은 광도 측정 (흡수, 형광) 으로 수행된다.
아미노산 분석에 관한 문헌이, 그 중에서도 Lottspeich 및 Zorbas (Spektrum Akademischer Verlag, Heidelberg, Germany 1998) 의 교과서 "Bioanalytik" 에서 확인될 수 있다.
따라서, 본 발명은 또한 하기 단계가 수행되는 것을 특징으로 하는 L-아미노산의 생산 방법에 관한 것이다:
a) 적합한 영양 배지에서의, 본 발명에 따른 미생물, 특히 코리네형 박테리아, 바람직하게는 코리네박테리움 속, 특히 바람직하게는 코리네박테리움 글루타미쿰 또는 코리네박테리움 휴미레듀센스 종의 코리네형 박테리아의 발효 단계, 및
b) 영양 배지 및/또는 언급된 박테리아의 세포에서의 L-아미노산의 축적 단계.
이어서, L-아미노산을 함유하는 생성물은 액체 또는 고체 형태로 제공되거나 생산되거나 회수된다.
발효 조치는 관련 L-아미노산을 포함하는 발효액을 수득하게 한다.
발효액은, 특정 시간 동안 및 특정 온도에서 미생물이 배양된 발효 배지 또는 영양 배지를 의미한다. 발효 중 사용된 발효 배지 또는 배지들은, 미생물의 번식 및 목적하는 L-아미노산의 형성을 보장하는 모든 물질 또는 성분을 포함한다.
따라서, 발효가 완료된 경우, 발효액은 다음을 포함한다:
a) 미생물의 바이오매스 (biomass) (세포 덩어리), 상기 바이오매스는 상기 미생물의 세포의 증식으로 인해 생산됨,
b) 발효 중 형성된 L-아미노산,
c) 발효 중 형성된 유기 부산물, 및
d) 이용된 발효 배지 또는 개시 물질의 구성요소, 예를 들어 비오틴과 같은 비타민 또는 마그네슘 술페이트와 같은 염 (이는 발효 중 소비되지 않음).
유기 부산물은 목적하는 L-아미노산에 더불어 발효 중 이용된 미생물에 의해 생성되고, 임의로 분비되는 물질을 포함한다. 이는 또한, 예를 들어 트레할로오스와 같은 당을 포함한다.
발효액은 배양 용기 또는 발효 탱크에서 제거되고, 적절한 곳에 수집되고, 액체 또는 고체 형태로, L-아미노산-함유 생성물을 제공하는데 사용된다. 또한, 표현 "L-아미노산-함유 생성물을 회수함" 이 이에 사용된다. 가장 간단한 경우, L-아미노산-함유 발효액 그 자체가 회수된 생성물을 구성한다.
a) 물의 부분 (> 0% 내지 < 80%) 내지 완전 (100%) 또는 사실상 완전 (≥ 80%, ≥ 90%, ≥ 95%, ≥ 96%, ≥ 97%, ≥ 98%, ≥ 99%) 제거,
b) 바이오매스의 부분 (> 0% 내지 < 80%) 내지 완전 (100%) 또는 사실상 완전 (≥ 80%, ≥ 90%, ≥ 95%, ≥ 96%, ≥ 97%, ≥ 98%, ≥ 99%) 제거, 후자는 제거 전 임의로 불활성화됨,
c) 발효 중 형성된 유기 부산물의 부분 (> 0% 내지 < 80%) 내지 완전 (100%) 또는 사실상 완전 (≥ 80%, ≥ 90%, ≥ 95%, ≥ 96%, ≥ 97%, ≥ 98%, ≥ 99%, ≥ 99.3%, ≥ 99.7%) 제거, 및
d) 이용된 발효 배지 또는 개시 물질의 구성요소 (이는 발효 중 소비되지 않음) 의 부분 (> 0%) 내지 완전 (100%) 또는 사실상 완전 (≥ 80%, ≥ 90%, ≥ 95%, ≥ 96%, ≥ 97%, ≥ 98%, ≥ 99%, ≥ 99.3%, ≥ 99.7%) 제거
로 이루어진 군으로부터 선택되는, 발효액으로부터의 조치 중 하나 이상은 L-아미노산의 농축 또는 정제를 달성한다. 바람직한 L-아미노산 함량을 갖는 생성물은 이러한 방식으로 단리된다.
또한, 물의 부분 (> 0% 내지 < 80%) 내지 완전 (100%) 또는 사실상 완전 (≥ 80% 내지 < 100%) 제거 (조치 a)) 는 건조로서 간주된다.
물, 바이오매스, 유기 부산물 및 이용된 발효 배지의 소비되지 않은 구성요소의 완전 또는 사실상 완전 제거는, 순수 (≥ 80중량%, ≥ 90중량%) 또는 고순도 (≥ 95중량%, ≥ 97중량%, ≥ 99중량%) 생성물 형태의 L-아미노산을 수득하게 한다. 조치 a), b), c) 및 d) 에 대한 다수의 기술 지침이 선행 기술에서 이용가능하다.
작업예
실시예 1: C. 휴미레듀센스에 의한 알라닌 및 발린의 생산
L-알라닌 및 발린의 생산을 평가하기 위해, C. 휴미레듀센스 (DSM 45392) 균주를 진탕 플라스크 배치에서 배양하였다. 이러한 목적을 위해 C. 휴미레듀센스 균주를, 사전배양액으로서 24 시간 동안, 200 rpm, 37℃ 에서, 10 ml 의 BHI 액체 배지 (Brain Heart Infusion; Merck) (37 g/l 의 H2O) 중에서 인큐베이팅하였다. 이어서, 10 ml의 진탕 플라스크 배지를 OD660 이 0.2 이 되도록 접종하고, 48 시간 동안, 200 rpm, 37℃ 에서 배양하였다. 상기 배지를 제조하기 위해, 20 g 의 암모늄 술페이트, 0.4 g 의 MgSO4*7H2O, 0.6 g 의 KH2PO4 및 10 g 의 효모 추출물을 750 ml 의 H2O 중에 용해시켰다. 20% NH4OH 를 이용하여, 용액의 pH 를 7.8 로 조정한 다음, 용액을 오토클레이브 처리하였다. 이어서, 0.25 g/l 의 티아민, 50 mg/l 의 시아노코발라민, 25 mg/l 의 비오틴 및 1.25 g/l 의 피리독신으로 이루어진 4 ml 의 비타민 용액 (NH4OH 를 이용하여 pH 7 임) 을 첨가하였다. 또한, 140 ml 의 멸균 여과된 (sterile-filtered) 50% 글루코오스 용액 및 50 g 의 오토클레이브 처리된 무수 CaCO3 를 첨가하고, 이어서 배지를 1 리터로 만들었다.
배양 후, 4 개의 평행 배양의 상등액을, 각각의 경우 HPLC 로 분석하여 알라닌, 글리신 및 발린 함량을 측정하였다 (검출 한계 ≥0.01 g/l).
48 시간 동안, 37℃, 200 rpm 에서의 진탕 플라스크 스케일에서, 진탕 플라스크 배지 중 배양 후, C. 휴미레듀센스 균주는 주위에 0.81 g/l 의 알라닌 (순 수율: 0.011 g알라닌/g글루코오스) 및 1.6 g/l 의 발린 (순 수율: 0.022 g발린/g글루코오스) (표 1) 을 생산하였다. 글리신은 부산물로서 단지 소량만 생산되었다.
표 1: C. 휴미레듀센스 균주를 이용한 진탕 플라스크 실험으로부터의 분석적 데이터. 세포 및 공 배지로 배양한 후 측정한 값을 제시함.
Figure pct00001
실시예 3: 글루타메이트 수행성 검정
L-글루타메이트 성능 검정을 위해, C. 휴미레듀센스 (DSM 45392) 균주를 진탕 플라스크 배치에서 배양하였다. 이러한 목적을 위해, C. 휴미레듀센스 균주를, 사전배양액으로서 24 시간 동안, 200 rpm, 37℃ 에서, 10 ml 의 BHI 액체 배지 (Brain Heart Infusion; Merck) (37 g/l 의 H2O) 중에서 인큐베이팅하였다. 이어서, 10 ml의 진탕 플라스크 배지를 OD660 이 0.2 가 되도록 접종하고, 48 시간 동안, 200 rpm, 37℃ 에서 배양하였다. 상기 배지를 제조하기 위해, 20 g 의 암모늄 술페이트, 0.4 g 의 MgSO4*7H2O, 0.6 g 의 KH2PO4 및 10 g 의 효모 추출물을 750 ml 의 H2O 중에 용해시켰다. 20% NH4OH 를 이용하여, 용액의 pH 를 7.8 로 조정한 다음, 용액을 오토클레이브 처리하였다. 이어서, 0.25 g/l 의 티아민, 50 mg/l 의 시아노코발라민, 25 mg/l 의 비오틴 및 1.25 g/l 의 피리독신으로 이루어진 4 ml 의 비타민 용액 (NH4OH 를 이용하여 pH 7 임) 을 첨가하였다. 또한, 140 ml 의 멸균 여과된 50% 글루코오스 용액 및 50 g 의 오토클레이브 처리된 무수 CaCO3 를 첨가하였다. 이어서, 5 ml 의 400 mM 멸균 여과된 트레오닌 스톡 용액을 첨가한 다음, 이어서 배지를 1 리터로 만들었다.
배양 후, 4 개의 평행 배양의 상등액을, 각각의 경우 HPLC 로 분석하여 글루타메이트 함량을 측정하였다 (검출 한계 ≥0.01 g/l).
48 시간 동안, 37℃, 200 rpm 에서의 진탕 플라스크 스케일에서, 진탕 플라스크 배지 중 배양 후, C. 휴미레듀센스 균주는 1.8 (+/-0.6) g/l 의 L-글루타메이트를 생산하였다. 배지 중 L-글루타메이트의 초기 농도는 0.78 (+/-0.1) g/l 였다. 글리신은 부산물로서 단지 소량만 생산되었다.
SEQUENCE LISTING <110> Evonik Industries AG <120> Method for producing L-amino acids in Corynebacteria using a glycine cleavage system <130> 201400112 <160> 202 <170> PatentIn version 3.5 <210> 1 <211> 1219 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(918) <223> gene mcbR <400> 1 ctactcggtg gcttcctcct cagcctcctg ggggtcgacg tggccggcgg aggcctgatc 60 ttcagcttcc tcacctgtct gctcggtgcg gtgatcctgc tgaccatcgt caacatggtg 120 accggtcgca gccggagcta gcctgcgggt ccccgcatga acccgcccct cggggcgggt 180 tttttcatgc ccgtatgcag ggcgactgtc aaaagacgta ccggtttgtc tatattgtgt 240 gggagaggta gtcccgaacg atcccggaaa ggaaccggta cccacgtgtc cgcaggaaca 300 gtg agg aag aag acg acg acc gcg cgg cgt cgc aac cga ccg agc ccg 348 Val Arg Lys Lys Thr Thr Thr Ala Arg Arg Arg Asn Arg Pro Ser Pro 1 5 10 15 cgt cag cga ctg ctc gac tcg gcg acc aac ctg ttc acc acg gag ggc 396 Arg Gln Arg Leu Leu Asp Ser Ala Thr Asn Leu Phe Thr Thr Glu Gly 20 25 30 atc cgg gtc atc ggc atc gac cgg atc ctg cgg gag gcg gac gtc gcc 444 Ile Arg Val Ile Gly Ile Asp Arg Ile Leu Arg Glu Ala Asp Val Ala 35 40 45 aag gcg tcc ctg tac tcc ctc ttc ggc tcg aag gac gcc ctg gtc atc 492 Lys Ala Ser Leu Tyr Ser Leu Phe Gly Ser Lys Asp Ala Leu Val Ile 50 55 60 tcc tac ctg gag aac ctc gac gag cag tac cgg acg gcc tgg gag gag 540 Ser Tyr Leu Glu Asn Leu Asp Glu Gln Tyr Arg Thr Ala Trp Glu Glu 65 70 75 80 cgg acg aag ggg atg acg gac gcc gag gac aag atc ctc gcc ttc ttc 588 Arg Thr Lys Gly Met Thr Asp Ala Glu Asp Lys Ile Leu Ala Phe Phe 85 90 95 gac ctc gcc atc gcg gag gag ccg ggc aag gag ttc cgc ggc tcc cac 636 Asp Leu Ala Ile Ala Glu Glu Pro Gly Lys Glu Phe Arg Gly Ser His 100 105 110 ttc cag aac gcc gcc aac gag tac ccg cgc ccg gag acc gag tcc gag 684 Phe Gln Asn Ala Ala Asn Glu Tyr Pro Arg Pro Glu Thr Glu Ser Glu 115 120 125 cac ggc atc gtc gcc gcc tgc cgg gag cac cgc cgc tgg atg cac cag 732 His Gly Ile Val Ala Ala Cys Arg Glu His Arg Arg Trp Met His Gln 130 135 140 acg atg acg gac ctg ctc acc gcg aag aac ggc tac ccc tcc gcc acg 780 Thr Met Thr Asp Leu Leu Thr Ala Lys Asn Gly Tyr Pro Ser Ala Thr 145 150 155 160 cag gcg aac cag ctg ctc atc ttc ctc gac ggc ggc ctg gcg ggc gcg 828 Gln Ala Asn Gln Leu Leu Ile Phe Leu Asp Gly Gly Leu Ala Gly Ala 165 170 175 cgc ttc gcc cgg gag gtc act ccg ctg cag acg gcc cgt gat ctg gcc 876 Arg Phe Ala Arg Glu Val Thr Pro Leu Gln Thr Ala Arg Asp Leu Ala 180 185 190 cgt cag atg ctg tcg gct cct ccg gcg gac tac tcg atc tag 918 Arg Gln Met Leu Ser Ala Pro Pro Ala Asp Tyr Ser Ile 195 200 205 cgttcttccg cgcctccagc ttcgccctcc accgctcgac gcgggcgttc gtctcctccc 978 gctgcagggc gctgcgggcc tgctgacgct ccttctcgag cgccttccgc tccgccttct 1038 ccgccgccag ccgctcgcga cgttcggcct tctcggcctt cagttcgcgg ctgatctcgg 1098 ggatccgcca cggctgcacg gcgcccactg ctttccgacg ccgcgcgtcc cacttcacct 1158 gccgcgcctc acgcgccgcc gcccgggcct tctcccggcc ctgccggtgc agctcgtgga 1218 a 1219 <210> 2 <211> 205 <212> PRT <213> Corynebacterium humireducens <400> 2 Val Arg Lys Lys Thr Thr Thr Ala Arg Arg Arg Asn Arg Pro Ser Pro 1 5 10 15 Arg Gln Arg Leu Leu Asp Ser Ala Thr Asn Leu Phe Thr Thr Glu Gly 20 25 30 Ile Arg Val Ile Gly Ile Asp Arg Ile Leu Arg Glu Ala Asp Val Ala 35 40 45 Lys Ala Ser Leu Tyr Ser Leu Phe Gly Ser Lys Asp Ala Leu Val Ile 50 55 60 Ser Tyr Leu Glu Asn Leu Asp Glu Gln Tyr Arg Thr Ala Trp Glu Glu 65 70 75 80 Arg Thr Lys Gly Met Thr Asp Ala Glu Asp Lys Ile Leu Ala Phe Phe 85 90 95 Asp Leu Ala Ile Ala Glu Glu Pro Gly Lys Glu Phe Arg Gly Ser His 100 105 110 Phe Gln Asn Ala Ala Asn Glu Tyr Pro Arg Pro Glu Thr Glu Ser Glu 115 120 125 His Gly Ile Val Ala Ala Cys Arg Glu His Arg Arg Trp Met His Gln 130 135 140 Thr Met Thr Asp Leu Leu Thr Ala Lys Asn Gly Tyr Pro Ser Ala Thr 145 150 155 160 Gln Ala Asn Gln Leu Leu Ile Phe Leu Asp Gly Gly Leu Ala Gly Ala 165 170 175 Arg Phe Ala Arg Glu Val Thr Pro Leu Gln Thr Ala Arg Asp Leu Ala 180 185 190 Arg Gln Met Leu Ser Ala Pro Pro Ala Asp Tyr Ser Ile 195 200 205 <210> 3 <211> 1527 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1227) <223> gene thrB <400> 3 agaacaccta cgcgaacctg ccgatcgccg acctcggcga cgtgcgcacc cgctaccaca 60 tcgacatgga ggtcgaggac cgcaccggtg tcctcgccga gctgtccgcg atcttcgcca 120 cccacgacat ctccctcaag accgtccgcc aggaggaggc cggcgaccac gcccgcctca 180 tcgtcgtcac gcattccgca cgtgaggccg acctggccgc caccgtcgag gacctgaaga 240 aggccccggc ggtcaagtcc gtcgacagcg tcctgcgact catctaggga gcgttgagct 300 gtg agc atc cag ctt gag gtg ggc acc cgg gcc acc gtc acc gtc ccc 348 Val Ser Ile Gln Leu Glu Val Gly Thr Arg Ala Thr Val Thr Val Pro 1 5 10 15 ggc tcc tcc gcg aac ctg ggc ccc gga ttc gac acc ctc ggc ctg gcc 396 Gly Ser Ser Ala Asn Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala 20 25 30 gtg ggc atc tac gac acc gtc gag gtc gag gtc atc gag tcg ggc ctc 444 Val Gly Ile Tyr Asp Thr Val Glu Val Glu Val Ile Glu Ser Gly Leu 35 40 45 gag gtc gag gtc ttc ggc gag ggc gag gac gac ctg ccc cgc gac ggt 492 Glu Val Glu Val Phe Gly Glu Gly Glu Asp Asp Leu Pro Arg Asp Gly 50 55 60 tcc cac ctg gtg gtc aag gcc atc cgg tcg ggc ctc aac gcc gcc cac 540 Ser His Leu Val Val Lys Ala Ile Arg Ser Gly Leu Asn Ala Ala His 65 70 75 80 gcc gag gcc ccg ggc ctg cgc gtg gtg tgc acc aac gcc atc ccg cag 588 Ala Glu Ala Pro Gly Leu Arg Val Val Cys Thr Asn Ala Ile Pro Gln 85 90 95 tcc cgt ggc ctg ggc tcc tcc gcc gcc gca gcc gtc gcg gga gtt ctg 636 Ser Arg Gly Leu Gly Ser Ser Ala Ala Ala Ala Val Ala Gly Val Leu 100 105 110 gcc gcc aac ggt ctg gcg ggc aac ccg ctg acc acc gac cag gtg gtc 684 Ala Ala Asn Gly Leu Ala Gly Asn Pro Leu Thr Thr Asp Gln Val Val 115 120 125 cag ctg tcc tcc gcc ttc gag ggg cac ccc gac aac gcc gcg gcc tcc 732 Gln Leu Ser Ser Ala Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser 130 135 140 gtc ctc ggt tcc gcc gtg gtc tcc tgg acc acg acc ccg gtc gac ggc 780 Val Leu Gly Ser Ala Val Val Ser Trp Thr Thr Thr Pro Val Asp Gly 145 150 155 160 tcg cag ccg ggg tac cgg gcg gtg gcg gtg gac gtc gac aag cgc atc 828 Ser Gln Pro Gly Tyr Arg Ala Val Ala Val Asp Val Asp Lys Arg Ile 165 170 175 cgg gcg acg gct ctc gtg ccg gac ttc cac gcc tcc acc cag gcg gtg 876 Arg Ala Thr Ala Leu Val Pro Asp Phe His Ala Ser Thr Gln Ala Val 180 185 190 cgt cgc gtg ctg ccg agc cac gtc acc cac acg gac gcg cgt ttc aac 924 Arg Arg Val Leu Pro Ser His Val Thr His Thr Asp Ala Arg Phe Asn 195 200 205 gtc tcg cgc acc gcg gtg atg acc gtg gcg ctg cag aac cac ccg gag 972 Val Ser Arg Thr Ala Val Met Thr Val Ala Leu Gln Asn His Pro Glu 210 215 220 ctg ctg tgg gag ggc acc cgt gac cgc ctg cac cag ccc tac cgc gcc 1020 Leu Leu Trp Glu Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg Ala 225 230 235 240 gac gtg ctg ccg gtg acc gcc gag tgg gtc aac cgt ctg cgc aac cgc 1068 Asp Val Leu Pro Val Thr Ala Glu Trp Val Asn Arg Leu Arg Asn Arg 245 250 255 ggt tac gcc gcg tac ctc tcc ggc gcc ggc ccg acc tgc atg gtc ctg 1116 Gly Tyr Ala Ala Tyr Leu Ser Gly Ala Gly Pro Thr Cys Met Val Leu 260 265 270 tcg gtc gac ccg atc gag gag gcc atc ctc gag gag gcc cgc gcc gcc 1164 Ser Val Asp Pro Ile Glu Glu Ala Ile Leu Glu Glu Ala Arg Ala Ala 275 280 285 ggt ctg cgc gtc ctc gag ctg gag gtc gcc ggt ccg gcg acc gtc gag 1212 Gly Leu Arg Val Leu Glu Leu Glu Val Ala Gly Pro Ala Thr Val Glu 290 295 300 gtc agc cgg gtc tga cctaccggtc gtcggcgggg gtccccctgt cggcgaccac 1267 Val Ser Arg Val 305 cgagcacgcg cccgagggct ggaaggggcg caggctcagc cggacggggg cggagccgac 1327 ggactcctgc aggaaaccct cgtggatggc gcagatcatg agcgagggtc ggtgcccctc 1387 ggcgacgaag gggcacgcct gcagctcgat gtcgaccgac tccgcgtcgg tgcccccgtc 1447 gccctgctcc ctgcgcatgg tcgggtcgaa acccagctca cggagggtgt ggtggagacg 1507 gtcgagcgcc gtgtccatgg 1527 <210> 4 <211> 308 <212> PRT <213> Corynebacterium humireducens <400> 4 Val Ser Ile Gln Leu Glu Val Gly Thr Arg Ala Thr Val Thr Val Pro 1 5 10 15 Gly Ser Ser Ala Asn Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala 20 25 30 Val Gly Ile Tyr Asp Thr Val Glu Val Glu Val Ile Glu Ser Gly Leu 35 40 45 Glu Val Glu Val Phe Gly Glu Gly Glu Asp Asp Leu Pro Arg Asp Gly 50 55 60 Ser His Leu Val Val Lys Ala Ile Arg Ser Gly Leu Asn Ala Ala His 65 70 75 80 Ala Glu Ala Pro Gly Leu Arg Val Val Cys Thr Asn Ala Ile Pro Gln 85 90 95 Ser Arg Gly Leu Gly Ser Ser Ala Ala Ala Ala Val Ala Gly Val Leu 100 105 110 Ala Ala Asn Gly Leu Ala Gly Asn Pro Leu Thr Thr Asp Gln Val Val 115 120 125 Gln Leu Ser Ser Ala Phe Glu Gly His Pro Asp Asn Ala Ala Ala Ser 130 135 140 Val Leu Gly Ser Ala Val Val Ser Trp Thr Thr Thr Pro Val Asp Gly 145 150 155 160 Ser Gln Pro Gly Tyr Arg Ala Val Ala Val Asp Val Asp Lys Arg Ile 165 170 175 Arg Ala Thr Ala Leu Val Pro Asp Phe His Ala Ser Thr Gln Ala Val 180 185 190 Arg Arg Val Leu Pro Ser His Val Thr His Thr Asp Ala Arg Phe Asn 195 200 205 Val Ser Arg Thr Ala Val Met Thr Val Ala Leu Gln Asn His Pro Glu 210 215 220 Leu Leu Trp Glu Gly Thr Arg Asp Arg Leu His Gln Pro Tyr Arg Ala 225 230 235 240 Asp Val Leu Pro Val Thr Ala Glu Trp Val Asn Arg Leu Arg Asn Arg 245 250 255 Gly Tyr Ala Ala Tyr Leu Ser Gly Ala Gly Pro Thr Cys Met Val Leu 260 265 270 Ser Val Asp Pro Ile Glu Glu Ala Ile Leu Glu Glu Ala Arg Ala Ala 275 280 285 Gly Leu Arg Val Leu Glu Leu Glu Val Ala Gly Pro Ala Thr Val Glu 290 295 300 Val Ser Arg Val 305 <210> 5 <211> 2238 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1938) <223> gene pgi <400> 5 caggcgatgt aggactcctc gtcggcccag cgggtgacca cgaagtagcg gttctccccg 60 gccacggggc ggagaagctg gaaaccctcg aagccgggtg cctggtcgac ggcgttcttc 120 cgggcggcga agcgctcctc cagctgcggg ccggcgccct cggggacggt gatggcgttg 180 attttcacga tgctcatgcc ccccattgtg cccaaccggc cgcttccccg gcggacaccc 240 gggaaagtca ctagattcgt gagtgtcccg aacatatccc cgcattgagg agcccgaccc 300 atg gcc gac atc acc acc tcc gct gaa tgg aag aac ctg gag tcc gag 348 Met Ala Asp Ile Thr Thr Ser Ala Glu Trp Lys Asn Leu Glu Ser Glu 1 5 10 15 ttc gag cgc acc cag ggt cag acc ctg cgg gag ctg ttc gcc gcg gat 396 Phe Glu Arg Thr Gln Gly Gln Thr Leu Arg Glu Leu Phe Ala Ala Asp 20 25 30 ccg cag cgt gcg gag aag ctg acc ttc gat gcg gcc ggt ctc cac gtc 444 Pro Gln Arg Ala Glu Lys Leu Thr Phe Asp Ala Ala Gly Leu His Val 35 40 45 gac ctc tcc aag aac ctg ctt gac gac gcc acc gtg gcc tcc ctc gtc 492 Asp Leu Ser Lys Asn Leu Leu Asp Asp Ala Thr Val Ala Ser Leu Val 50 55 60 gcc ctc gcc aag gag gcg cag ctc acc gag gcc atc gag gcg atg ttc 540 Ala Leu Ala Lys Glu Ala Gln Leu Thr Glu Ala Ile Glu Ala Met Phe 65 70 75 80 agc ggc gtg cac atc aac aac acc gag gac cgc gcc gtg ctg cac acc 588 Ser Gly Val His Ile Asn Asn Thr Glu Asp Arg Ala Val Leu His Thr 85 90 95 gcc ctg cgt ctg ccg gtc gag gag gac ctc gag gtc gac ggc cag gac 636 Ala Leu Arg Leu Pro Val Glu Glu Asp Leu Glu Val Asp Gly Gln Asp 100 105 110 gtc gcc gcc gac gtc cac gag gtg ctc ggc cgc atg cgc gac ttc gcc 684 Val Ala Ala Asp Val His Glu Val Leu Gly Arg Met Arg Asp Phe Ala 115 120 125 tcc tcc ctg cgt gcg ggc acc tgg ctc ggc cac acc ggc cgc acc atc 732 Ser Ser Leu Arg Ala Gly Thr Trp Leu Gly His Thr Gly Arg Thr Ile 130 135 140 aag aag gtc gtc aac atc ggc atc ggc ggc tcc gac ctg ggc ccg gcc 780 Lys Lys Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly Pro Ala 145 150 155 160 atg gcc gcc cgg gcg ctg cgc tcc tac gcc acc gcc ggc atc acc gcc 828 Met Ala Ala Arg Ala Leu Arg Ser Tyr Ala Thr Ala Gly Ile Thr Ala 165 170 175 gag ttc gtc tcc aac gtc gac ccg gcc gac atg tcc gcc acc ctc gac 876 Glu Phe Val Ser Asn Val Asp Pro Ala Asp Met Ser Ala Thr Leu Asp 180 185 190 gag ctc gac ccc gag tcg acc ctg ttc atc atc gcc tcc aag acc ttc 924 Glu Leu Asp Pro Glu Ser Thr Leu Phe Ile Ile Ala Ser Lys Thr Phe 195 200 205 acc acc cag gag acc ctc gcc aac gcg cac gcc gcg aag cgc tgg ctg 972 Thr Thr Gln Glu Thr Leu Ala Asn Ala His Ala Ala Lys Arg Trp Leu 210 215 220 ctg cag gcc ttc gac ggg gac gag tcg gcc gtc gcg aag cac ttc gtg 1020 Leu Gln Ala Phe Asp Gly Asp Glu Ser Ala Val Ala Lys His Phe Val 225 230 235 240 gcg gtg tcc acc aac gcc cag aag gtc gcc gag ttc ggc atc gac acc 1068 Ala Val Ser Thr Asn Ala Gln Lys Val Ala Glu Phe Gly Ile Asp Thr 245 250 255 gac aac atg ttc ggc ttc tgg gac tgg gta ggc ggc cgc tac tcc gtc 1116 Asp Asn Met Phe Gly Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Val 260 265 270 gac tcc gcc atc ggc ctg tcc ctc atg gcc gtc atc ggc ccg ctg gac 1164 Asp Ser Ala Ile Gly Leu Ser Leu Met Ala Val Ile Gly Pro Leu Asp 275 280 285 ttc atg cgc ttc ctc gag ggc ttc cgc gcc atg gac gag cac ttc cgc 1212 Phe Met Arg Phe Leu Glu Gly Phe Arg Ala Met Asp Glu His Phe Arg 290 295 300 gcg acc ccc ttc gag aag aac gtg ccc gtc ctc atg gcg ctg ctg ggc 1260 Ala Thr Pro Phe Glu Lys Asn Val Pro Val Leu Met Ala Leu Leu Gly 305 310 315 320 atc tgg tac gtc aac ttc cac ggc ttc gag acc cac gcg gtc ctc ccc 1308 Ile Trp Tyr Val Asn Phe His Gly Phe Glu Thr His Ala Val Leu Pro 325 330 335 tac tct cag gac ctc tcc cgt ttc gcc gcc tac ctg cag cag ctg acc 1356 Tyr Ser Gln Asp Leu Ser Arg Phe Ala Ala Tyr Leu Gln Gln Leu Thr 340 345 350 atg gag tcc aac ggc aag tcc gtc cga cac gac ggc acc gcc gtc gac 1404 Met Glu Ser Asn Gly Lys Ser Val Arg His Asp Gly Thr Ala Val Asp 355 360 365 tac gcc acc ggt gag atc tac tgg ggc gag ccg ggc acc aac ggc cag 1452 Tyr Ala Thr Gly Glu Ile Tyr Trp Gly Glu Pro Gly Thr Asn Gly Gln 370 375 380 cac gcc ttc tac cag ctc atg cac cag ggc acc cgc gtc gtc ccg gcc 1500 His Ala Phe Tyr Gln Leu Met His Gln Gly Thr Arg Val Val Pro Ala 385 390 395 400 gac ttc atc ggc ttc gcc cgc ccg aag aac gac ctg ccg acc gcc acc 1548 Asp Phe Ile Gly Phe Ala Arg Pro Lys Asn Asp Leu Pro Thr Ala Thr 405 410 415 ggc gag ggc tcc atg cac gac ctg ctc atg ggc aac ctc ttc gcc cag 1596 Gly Glu Gly Ser Met His Asp Leu Leu Met Gly Asn Leu Phe Ala Gln 420 425 430 agc aag gtg ctg gcc ttc ggc aag acc gcc gag gag atc gcc gcc gag 1644 Ser Lys Val Leu Ala Phe Gly Lys Thr Ala Glu Glu Ile Ala Ala Glu 435 440 445 gga gtg tcc gcg gac ctc gtc gcc cac aag gtc atg ccg ggc aac cgc 1692 Gly Val Ser Ala Asp Leu Val Ala His Lys Val Met Pro Gly Asn Arg 450 455 460 ccg tcc acg acc atc ctc gcg gag gag ctc acc ccg gcg gtg ctc ggc 1740 Pro Ser Thr Thr Ile Leu Ala Glu Glu Leu Thr Pro Ala Val Leu Gly 465 470 475 480 gcg ctc atc gcc ctc tac gag cac atc acc ttc gtc cag ggc gtc atc 1788 Ala Leu Ile Ala Leu Tyr Glu His Ile Thr Phe Val Gln Gly Val Ile 485 490 495 tgg gac atc aac tcc ttc gac cag tgg ggc gtg gag ctg ggc aag cag 1836 Trp Asp Ile Asn Ser Phe Asp Gln Trp Gly Val Glu Leu Gly Lys Gln 500 505 510 cag gcc aac cag ctg gcc gcc gcc gtc tcc ggc gcc gag gag gtc gac 1884 Gln Ala Asn Gln Leu Ala Ala Ala Val Ser Gly Ala Glu Glu Val Asp 515 520 525 tcc ggc gac tcc tcc acc gac gcg ctc gtg cgc tgg tac cgc gcg aac 1932 Ser Gly Asp Ser Ser Thr Asp Ala Leu Val Arg Trp Tyr Arg Ala Asn 530 535 540 cgc tag ttcggcaggg ccggcatccc gaggggggtg ccggccctct gcttctcgac 1988 Arg 545 gtcgccccgt tccccgtaac tgaaccggaa ctcgtggaac ggcaggttca cggtctccag 2048 gtggtagaag gcgttccggg cgtgcttgga gtagtggtac tcgcgggccc ggagggtggt 2108 caccgacagc aggttgccgt agccgggcag cagcaggtgc gcgtactgcc ggaggaaggg 2168 caccaggtcg aggctccatc cctcccccgt gcgggtcagt tccgccgggt acaggtcgac 2228 ggtggccacg 2238 <210> 6 <211> 545 <212> PRT <213> Corynebacterium humireducens <400> 6 Met Ala Asp Ile Thr Thr Ser Ala Glu Trp Lys Asn Leu Glu Ser Glu 1 5 10 15 Phe Glu Arg Thr Gln Gly Gln Thr Leu Arg Glu Leu Phe Ala Ala Asp 20 25 30 Pro Gln Arg Ala Glu Lys Leu Thr Phe Asp Ala Ala Gly Leu His Val 35 40 45 Asp Leu Ser Lys Asn Leu Leu Asp Asp Ala Thr Val Ala Ser Leu Val 50 55 60 Ala Leu Ala Lys Glu Ala Gln Leu Thr Glu Ala Ile Glu Ala Met Phe 65 70 75 80 Ser Gly Val His Ile Asn Asn Thr Glu Asp Arg Ala Val Leu His Thr 85 90 95 Ala Leu Arg Leu Pro Val Glu Glu Asp Leu Glu Val Asp Gly Gln Asp 100 105 110 Val Ala Ala Asp Val His Glu Val Leu Gly Arg Met Arg Asp Phe Ala 115 120 125 Ser Ser Leu Arg Ala Gly Thr Trp Leu Gly His Thr Gly Arg Thr Ile 130 135 140 Lys Lys Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly Pro Ala 145 150 155 160 Met Ala Ala Arg Ala Leu Arg Ser Tyr Ala Thr Ala Gly Ile Thr Ala 165 170 175 Glu Phe Val Ser Asn Val Asp Pro Ala Asp Met Ser Ala Thr Leu Asp 180 185 190 Glu Leu Asp Pro Glu Ser Thr Leu Phe Ile Ile Ala Ser Lys Thr Phe 195 200 205 Thr Thr Gln Glu Thr Leu Ala Asn Ala His Ala Ala Lys Arg Trp Leu 210 215 220 Leu Gln Ala Phe Asp Gly Asp Glu Ser Ala Val Ala Lys His Phe Val 225 230 235 240 Ala Val Ser Thr Asn Ala Gln Lys Val Ala Glu Phe Gly Ile Asp Thr 245 250 255 Asp Asn Met Phe Gly Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Val 260 265 270 Asp Ser Ala Ile Gly Leu Ser Leu Met Ala Val Ile Gly Pro Leu Asp 275 280 285 Phe Met Arg Phe Leu Glu Gly Phe Arg Ala Met Asp Glu His Phe Arg 290 295 300 Ala Thr Pro Phe Glu Lys Asn Val Pro Val Leu Met Ala Leu Leu Gly 305 310 315 320 Ile Trp Tyr Val Asn Phe His Gly Phe Glu Thr His Ala Val Leu Pro 325 330 335 Tyr Ser Gln Asp Leu Ser Arg Phe Ala Ala Tyr Leu Gln Gln Leu Thr 340 345 350 Met Glu Ser Asn Gly Lys Ser Val Arg His Asp Gly Thr Ala Val Asp 355 360 365 Tyr Ala Thr Gly Glu Ile Tyr Trp Gly Glu Pro Gly Thr Asn Gly Gln 370 375 380 His Ala Phe Tyr Gln Leu Met His Gln Gly Thr Arg Val Val Pro Ala 385 390 395 400 Asp Phe Ile Gly Phe Ala Arg Pro Lys Asn Asp Leu Pro Thr Ala Thr 405 410 415 Gly Glu Gly Ser Met His Asp Leu Leu Met Gly Asn Leu Phe Ala Gln 420 425 430 Ser Lys Val Leu Ala Phe Gly Lys Thr Ala Glu Glu Ile Ala Ala Glu 435 440 445 Gly Val Ser Ala Asp Leu Val Ala His Lys Val Met Pro Gly Asn Arg 450 455 460 Pro Ser Thr Thr Ile Leu Ala Glu Glu Leu Thr Pro Ala Val Leu Gly 465 470 475 480 Ala Leu Ile Ala Leu Tyr Glu His Ile Thr Phe Val Gln Gly Val Ile 485 490 495 Trp Asp Ile Asn Ser Phe Asp Gln Trp Gly Val Glu Leu Gly Lys Gln 500 505 510 Gln Ala Asn Gln Leu Ala Ala Ala Val Ser Gly Ala Glu Glu Val Asp 515 520 525 Ser Gly Asp Ser Ser Thr Asp Ala Leu Val Arg Trp Tyr Arg Ala Asn 530 535 540 Arg 545 <210> 7 <211> 2430 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2130) <223> gene pck <400> 7 cgctatgaca tctggactac agacccgcac cgcgcggcct ggggtctggc ggtcccggaa 60 cctgtgaaac ctcagtcaga ggggcccctg gcgtggggtt tagccggtat ggtcaccccc 120 gaaagtgtgg cctccaacca gggcatttcc ggggaaaagg cacggtggcg gcggatcgac 180 ggcggataaa cccgctggat atgacctgtg acccgtacct catgtcctgt gagaccgtca 240 caaccgcgat ttaatggata ccctgtatag agagaactgc cgcaaacgtc aggagaacac 300 atg acc act gcc atc aag ggc ctc gtc ggc ccc gca ccg acc gat aac 348 Met Thr Thr Ala Ile Lys Gly Leu Val Gly Pro Ala Pro Thr Asp Asn 1 5 10 15 gag aag ctc ctc acc tgg atc gcc gag gca gtg gag ctg atg cag ccg 396 Glu Lys Leu Leu Thr Trp Ile Ala Glu Ala Val Glu Leu Met Gln Pro 20 25 30 gag aag gtc gtc ttc gcc gac ggc tcc cag gag gag tgg gac cgc ctc 444 Glu Lys Val Val Phe Ala Asp Gly Ser Gln Glu Glu Trp Asp Arg Leu 35 40 45 tcc gcc gag ctc gtg gag gca ggc acc ttc atc cgc ctc aac gag gag 492 Ser Ala Glu Leu Val Glu Ala Gly Thr Phe Ile Arg Leu Asn Glu Glu 50 55 60 aag cgc ccg aac agc ttc ctg gcc cgc tcc aac ccg gac gat gtc gcc 540 Lys Arg Pro Asn Ser Phe Leu Ala Arg Ser Asn Pro Asp Asp Val Ala 65 70 75 80 cgc gtc gag tcc cgc acc ttc atc tgc tcc gag aag gag gag gac gcc 588 Arg Val Glu Ser Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp Ala 85 90 95 ggc ccg acc aac aac tgg gcg gac ccg gtc gag atg aag aag gag atg 636 Gly Pro Thr Asn Asn Trp Ala Asp Pro Val Glu Met Lys Lys Glu Met 100 105 110 acc gag gta tac cgc ggc tcc atg aag ggc cgc acc atg tac gtc gtg 684 Thr Glu Val Tyr Arg Gly Ser Met Lys Gly Arg Thr Met Tyr Val Val 115 120 125 ccg ttc tgc atg ggc ccg atc acc gac ccg gag ccg aag ctc ggt gtc 732 Pro Phe Cys Met Gly Pro Ile Thr Asp Pro Glu Pro Lys Leu Gly Val 130 135 140 cag ctc acc gac tcc gcc tac gtc gtc ctc tcc atg cgc atc atg acc 780 Gln Leu Thr Asp Ser Ala Tyr Val Val Leu Ser Met Arg Ile Met Thr 145 150 155 160 cgt atg ggc tcc gag gcg ctc gag aag atc ggc acc gac ggc gat ttc 828 Arg Met Gly Ser Glu Ala Leu Glu Lys Ile Gly Thr Asp Gly Asp Phe 165 170 175 gtc cgc gca ctc cac tcc gtc ggc gcc ccg ctg gag ccg ggc cag gag 876 Val Arg Ala Leu His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln Glu 180 185 190 gac gtc atc tgg ccg tgc aac gac acc aag tac atc acc cag ttc ccg 924 Asp Val Ile Trp Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe Pro 195 200 205 gag acc aag gag atc tgg tcc tac ggc tcc ggt tac ggc ggc aac gcc 972 Glu Thr Lys Glu Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala 210 215 220 atc ctg gcc aag aag tgc tac gcc ctg cgc atc gcc tcc gtc atg gcc 1020 Ile Leu Ala Lys Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met Ala 225 230 235 240 aag gag gag ggc tgg atg gct gag cac atg ctc atc ctg aag ctc aac 1068 Lys Glu Glu Gly Trp Met Ala Glu His Met Leu Ile Leu Lys Leu Asn 245 250 255 tcc ccg gag ggc aag agc tac cac atc gcg gca gct ttc ccg tcc gcc 1116 Ser Pro Glu Gly Lys Ser Tyr His Ile Ala Ala Ala Phe Pro Ser Ala 260 265 270 tgc ggc aag acc aac ctc gcc atg atc acc ccg acc atc gag ggc tgg 1164 Cys Gly Lys Thr Asn Leu Ala Met Ile Thr Pro Thr Ile Glu Gly Trp 275 280 285 acc gcc gag gtc gtc ggc gac gac atc gcc tgg ctc aag ctg cgc gag 1212 Thr Ala Glu Val Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg Glu 290 295 300 gac ggc ctc tac gcc gtc aac ccg gag aac ggc ttc ttc ggc gtc gca 1260 Asp Gly Leu Tyr Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val Ala 305 310 315 320 ccg ggc acc aac tac gcc tcc aac ccg atc gcc atg gag acc atg gag 1308 Pro Gly Thr Asn Tyr Ala Ser Asn Pro Ile Ala Met Glu Thr Met Glu 325 330 335 ccg ggc aac acc ctg ttc acc aac gtc gca ctg acc gac gac ggt gac 1356 Pro Gly Asn Thr Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp 340 345 350 gtc tgg tgg gag ggc atg gac ggc gag aag ccg gag cac ctc atc gac 1404 Val Trp Trp Glu Gly Met Asp Gly Glu Lys Pro Glu His Leu Ile Asp 355 360 365 tgg ctg ggc aac gac tgg acc ccg gag tcc ggc acc aac gcc gca cac 1452 Trp Leu Gly Asn Asp Trp Thr Pro Glu Ser Gly Thr Asn Ala Ala His 370 375 380 ccg aac tcc cgc tac tgc gtg gcc atc gag cag tgc ccg gtc gcc gca 1500 Pro Asn Ser Arg Tyr Cys Val Ala Ile Glu Gln Cys Pro Val Ala Ala 385 390 395 400 ccg gag ttc aac gac ccg aag ggc gtc aag atc gac gcc atc ctc ttc 1548 Pro Glu Phe Asn Asp Pro Lys Gly Val Lys Ile Asp Ala Ile Leu Phe 405 410 415 ggt ggc cgt cgt gcc gac acc gtc ccg ctg gtc acc cag tcc tac gac 1596 Gly Gly Arg Arg Ala Asp Thr Val Pro Leu Val Thr Gln Ser Tyr Asp 420 425 430 tgg gag cac ggc acc ttc atc ggt gcg acc ctg gcc tcc ggc cag acc 1644 Trp Glu His Gly Thr Phe Ile Gly Ala Thr Leu Ala Ser Gly Gln Thr 435 440 445 gcc gca tcc gct gag gcg aag gtc ggc tcc ctg cgc cac gac ccg atg 1692 Ala Ala Ser Ala Glu Ala Lys Val Gly Ser Leu Arg His Asp Pro Met 450 455 460 gcc atg ctg ccg ttc atg ggc tac aac gcc ggt gac tac ctg cag cac 1740 Ala Met Leu Pro Phe Met Gly Tyr Asn Ala Gly Asp Tyr Leu Gln His 465 470 475 480 tgg atc gac atg ggc aag aag ggc ggc gac cgt atg ccg gcc atc ttc 1788 Trp Ile Asp Met Gly Lys Lys Gly Gly Asp Arg Met Pro Ala Ile Phe 485 490 495 ctg gtc aac tgg ttc cgc cgt ggc gag gac ggc cgc ttc ctg tgg ccg 1836 Leu Val Asn Trp Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp Pro 500 505 510 ggc ttc ggt gac aac tcc cgt gtc ctg aag tgg atc att gac cgc atc 1884 Gly Phe Gly Asp Asn Ser Arg Val Leu Lys Trp Ile Ile Asp Arg Ile 515 520 525 gag ggc aag gtc gac gcc gag gag acc gtc gtg ggc cac acc gcc cgc 1932 Glu Gly Lys Val Asp Ala Glu Glu Thr Val Val Gly His Thr Ala Arg 530 535 540 gcc gag gac ctc gac ctg acc ggc ctg gac acc ccg ctc gag gac gtc 1980 Ala Glu Asp Leu Asp Leu Thr Gly Leu Asp Thr Pro Leu Glu Asp Val 545 550 555 560 aag gag gcc ctg act gcg ccg gcc gca gac tgg gag cgc gac gtc gcc 2028 Lys Glu Ala Leu Thr Ala Pro Ala Ala Asp Trp Glu Arg Asp Val Ala 565 570 575 gac aac gag gag tac ctg gca ttc ctc ggc ccg cgt gtc ccg aac gag 2076 Asp Asn Glu Glu Tyr Leu Ala Phe Leu Gly Pro Arg Val Pro Asn Glu 580 585 590 gtc cac gag cag ctg ggc aag ctc aag gag cgc atc gag gcg aac aag 2124 Val His Glu Gln Leu Gly Lys Leu Lys Glu Arg Ile Glu Ala Asn Lys 595 600 605 aac tag ttcgtctccc ctcagaactt ccaccctcca ggttgactca gtccctgggg 2180 Asn ggtgggattt tttctgcccc ggtcgcaccg taagtccgag agaggatgtt cgagaggaga 2240 gtcctctcga acgtgcggga cccccgcggg actcctctcg acgatgtgat ctcgacgtca 2300 gggaagaggg gggaagggga acgccgtcag tgcttccgca gcacgaccac caggttcgac 2360 acgaggaact cccgcagcag cggcacccgc accatccacc acgcccacga ggggtggtag 2420 cgggggaagg 2430 <210> 8 <211> 609 <212> PRT <213> Corynebacterium humireducens <400> 8 Met Thr Thr Ala Ile Lys Gly Leu Val Gly Pro Ala Pro Thr Asp Asn 1 5 10 15 Glu Lys Leu Leu Thr Trp Ile Ala Glu Ala Val Glu Leu Met Gln Pro 20 25 30 Glu Lys Val Val Phe Ala Asp Gly Ser Gln Glu Glu Trp Asp Arg Leu 35 40 45 Ser Ala Glu Leu Val Glu Ala Gly Thr Phe Ile Arg Leu Asn Glu Glu 50 55 60 Lys Arg Pro Asn Ser Phe Leu Ala Arg Ser Asn Pro Asp Asp Val Ala 65 70 75 80 Arg Val Glu Ser Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp Ala 85 90 95 Gly Pro Thr Asn Asn Trp Ala Asp Pro Val Glu Met Lys Lys Glu Met 100 105 110 Thr Glu Val Tyr Arg Gly Ser Met Lys Gly Arg Thr Met Tyr Val Val 115 120 125 Pro Phe Cys Met Gly Pro Ile Thr Asp Pro Glu Pro Lys Leu Gly Val 130 135 140 Gln Leu Thr Asp Ser Ala Tyr Val Val Leu Ser Met Arg Ile Met Thr 145 150 155 160 Arg Met Gly Ser Glu Ala Leu Glu Lys Ile Gly Thr Asp Gly Asp Phe 165 170 175 Val Arg Ala Leu His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln Glu 180 185 190 Asp Val Ile Trp Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe Pro 195 200 205 Glu Thr Lys Glu Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala 210 215 220 Ile Leu Ala Lys Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met Ala 225 230 235 240 Lys Glu Glu Gly Trp Met Ala Glu His Met Leu Ile Leu Lys Leu Asn 245 250 255 Ser Pro Glu Gly Lys Ser Tyr His Ile Ala Ala Ala Phe Pro Ser Ala 260 265 270 Cys Gly Lys Thr Asn Leu Ala Met Ile Thr Pro Thr Ile Glu Gly Trp 275 280 285 Thr Ala Glu Val Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg Glu 290 295 300 Asp Gly Leu Tyr Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val Ala 305 310 315 320 Pro Gly Thr Asn Tyr Ala Ser Asn Pro Ile Ala Met Glu Thr Met Glu 325 330 335 Pro Gly Asn Thr Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp 340 345 350 Val Trp Trp Glu Gly Met Asp Gly Glu Lys Pro Glu His Leu Ile Asp 355 360 365 Trp Leu Gly Asn Asp Trp Thr Pro Glu Ser Gly Thr Asn Ala Ala His 370 375 380 Pro Asn Ser Arg Tyr Cys Val Ala Ile Glu Gln Cys Pro Val Ala Ala 385 390 395 400 Pro Glu Phe Asn Asp Pro Lys Gly Val Lys Ile Asp Ala Ile Leu Phe 405 410 415 Gly Gly Arg Arg Ala Asp Thr Val Pro Leu Val Thr Gln Ser Tyr Asp 420 425 430 Trp Glu His Gly Thr Phe Ile Gly Ala Thr Leu Ala Ser Gly Gln Thr 435 440 445 Ala Ala Ser Ala Glu Ala Lys Val Gly Ser Leu Arg His Asp Pro Met 450 455 460 Ala Met Leu Pro Phe Met Gly Tyr Asn Ala Gly Asp Tyr Leu Gln His 465 470 475 480 Trp Ile Asp Met Gly Lys Lys Gly Gly Asp Arg Met Pro Ala Ile Phe 485 490 495 Leu Val Asn Trp Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp Pro 500 505 510 Gly Phe Gly Asp Asn Ser Arg Val Leu Lys Trp Ile Ile Asp Arg Ile 515 520 525 Glu Gly Lys Val Asp Ala Glu Glu Thr Val Val Gly His Thr Ala Arg 530 535 540 Ala Glu Asp Leu Asp Leu Thr Gly Leu Asp Thr Pro Leu Glu Asp Val 545 550 555 560 Lys Glu Ala Leu Thr Ala Pro Ala Ala Asp Trp Glu Arg Asp Val Ala 565 570 575 Asp Asn Glu Glu Tyr Leu Ala Phe Leu Gly Pro Arg Val Pro Asn Glu 580 585 590 Val His Glu Gln Leu Gly Lys Leu Lys Glu Arg Ile Glu Ala Asn Lys 595 600 605 Asn <210> 9 <211> 1461 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1161) <223> gene metQ <400> 9 aagctgtagg agctcacggc gtggagctcc gcgaagggga gggcggaggg ggcgacgggg 60 cggcgtcggc aagcaccctc cccgagatgg ccgaccggga cgggcgtggg gcccgggcgg 120 ccggagagga tgcgctccag ccgcgaccag ccgaggtgtg ccccaccgtg gaatcccatg 180 ccccgcaatc tacccctgat cgaacagatg tgccacactg cccgcggcaa ggactagaca 240 gagcggtacg cccggtgcta ggtttcagaa ccaacgaacc gaacacgtaa ggacaccacc 300 atg act ttc cgt cgc ctc ctg gcc ggc acc gcc gcc gcc gtc atc gcc 348 Met Thr Phe Arg Arg Leu Leu Ala Gly Thr Ala Ala Ala Val Ile Ala 1 5 10 15 tcc gtg ggc ctc gtc gcc tgc ggc tcc ggc tcc tcc ggg gag acc gtc 396 Ser Val Gly Leu Val Ala Cys Gly Ser Gly Ser Ser Gly Glu Thr Val 20 25 30 cgc atc ggc acc acc gac gcc cag ctc aag gag tgg gac gtc ttc gcc 444 Arg Ile Gly Thr Thr Asp Ala Gln Leu Lys Glu Trp Asp Val Phe Ala 35 40 45 gac atc gtc gag gcg gag ggc atc gac atc gag atc gtg ccg ttc tcc 492 Asp Ile Val Glu Ala Glu Gly Ile Asp Ile Glu Ile Val Pro Phe Ser 50 55 60 gac tac aac acc ccg aac gac gcg ctc gtg cag ggt gag atc gac gtc 540 Asp Tyr Asn Thr Pro Asn Asp Ala Leu Val Gln Gly Glu Ile Asp Val 65 70 75 80 aac aag ttc cag cac ctg gac ttc ctc gcc tcc tac aac aaa ggc gcc 588 Asn Lys Phe Gln His Leu Asp Phe Leu Ala Ser Tyr Asn Lys Gly Ala 85 90 95 aac gcc gac ctc gtg ccg tac gcc gcc acc gag atc aac ccg ctg tcc 636 Asn Ala Asp Leu Val Pro Tyr Ala Ala Thr Glu Ile Asn Pro Leu Ser 100 105 110 ctg ttc tgg aag gac cac gac tcc ctc gac ggc atc gag ggc gag ggc 684 Leu Phe Trp Lys Asp His Asp Ser Leu Asp Gly Ile Glu Gly Glu Gly 115 120 125 gtc gtc atc ccc aac gac gac acc aac cag ggc cgc gcc atc aac gtc 732 Val Val Ile Pro Asn Asp Asp Thr Asn Gln Gly Arg Ala Ile Asn Val 130 135 140 ctc gcc cag gcc ggt ctg gtg acc ctg cgc gac gac agc gtc tac aac 780 Leu Ala Gln Ala Gly Leu Val Thr Leu Arg Asp Asp Ser Val Tyr Asn 145 150 155 160 ccg acc ccg gcc gac gtc gac acc gag aag tcc aag gtc tcc atc gtc 828 Pro Thr Pro Ala Asp Val Asp Thr Glu Lys Ser Lys Val Ser Ile Val 165 170 175 acc gtc gac gcc gcc cag acc acc acc gcc tac cac gag ggt cgc ccg 876 Thr Val Asp Ala Ala Gln Thr Thr Thr Ala Tyr His Glu Gly Arg Pro 180 185 190 gcc atc atc aac aac tcc ttc ctg gag cgc gcc agc atc gac ccg tcc 924 Ala Ile Ile Asn Asn Ser Phe Leu Glu Arg Ala Ser Ile Asp Pro Ser 195 200 205 ctc gcc atc ttc cag gat gac ccg agc acc gag cag gcc gag ccg tac 972 Leu Ala Ile Phe Gln Asp Asp Pro Ser Thr Glu Gln Ala Glu Pro Tyr 210 215 220 atc aac gcc tgg gtg acc acc cgc gac aag gcc gac gac gag acc ctc 1020 Ile Asn Ala Trp Val Thr Thr Arg Asp Lys Ala Asp Asp Glu Thr Leu 225 230 235 240 atc cgt ctc gcc gag ctg tgg aag gac ccg gcc gtc acc gcc gcc gtc 1068 Ile Arg Leu Ala Glu Leu Trp Lys Asp Pro Ala Val Thr Ala Ala Val 245 250 255 ctg gag acc tcc ggc aac acc gcc gtc acc gtc gag cgc tcg ccc gag 1116 Leu Glu Thr Ser Gly Asn Thr Ala Val Thr Val Glu Arg Ser Pro Glu 260 265 270 gag ctc gcc gcg atc atg gag cgc ctc gcg gcc cgc gag cag tag 1161 Glu Leu Ala Ala Ile Met Glu Arg Leu Ala Ala Arg Glu Gln 275 280 285 gccgcactcc gcccacctgc cccgtcgcgt tcccgcggcg gggtttagca tttacctgac 1221 cagtccccct cccagaacgg cccccagaac accgtgaccg aacctgtgac cgcaccaccc 1281 acccggccgc gtaccggtac ccgcatcgag ttccgcgatg tgaccaaggt cttcaccaac 1341 cagcggaaga ccgagacccg cgccgtcgac ggcgtcaccc tgaccgtcga acccggcgag 1401 atcctcggcg tcatcggcta ctccggcgcc ggcaagtcga cgctcgtccg cctcatcaac 1461 <210> 10 <211> 286 <212> PRT <213> Corynebacterium humireducens <400> 10 Met Thr Phe Arg Arg Leu Leu Ala Gly Thr Ala Ala Ala Val Ile Ala 1 5 10 15 Ser Val Gly Leu Val Ala Cys Gly Ser Gly Ser Ser Gly Glu Thr Val 20 25 30 Arg Ile Gly Thr Thr Asp Ala Gln Leu Lys Glu Trp Asp Val Phe Ala 35 40 45 Asp Ile Val Glu Ala Glu Gly Ile Asp Ile Glu Ile Val Pro Phe Ser 50 55 60 Asp Tyr Asn Thr Pro Asn Asp Ala Leu Val Gln Gly Glu Ile Asp Val 65 70 75 80 Asn Lys Phe Gln His Leu Asp Phe Leu Ala Ser Tyr Asn Lys Gly Ala 85 90 95 Asn Ala Asp Leu Val Pro Tyr Ala Ala Thr Glu Ile Asn Pro Leu Ser 100 105 110 Leu Phe Trp Lys Asp His Asp Ser Leu Asp Gly Ile Glu Gly Glu Gly 115 120 125 Val Val Ile Pro Asn Asp Asp Thr Asn Gln Gly Arg Ala Ile Asn Val 130 135 140 Leu Ala Gln Ala Gly Leu Val Thr Leu Arg Asp Asp Ser Val Tyr Asn 145 150 155 160 Pro Thr Pro Ala Asp Val Asp Thr Glu Lys Ser Lys Val Ser Ile Val 165 170 175 Thr Val Asp Ala Ala Gln Thr Thr Thr Ala Tyr His Glu Gly Arg Pro 180 185 190 Ala Ile Ile Asn Asn Ser Phe Leu Glu Arg Ala Ser Ile Asp Pro Ser 195 200 205 Leu Ala Ile Phe Gln Asp Asp Pro Ser Thr Glu Gln Ala Glu Pro Tyr 210 215 220 Ile Asn Ala Trp Val Thr Thr Arg Asp Lys Ala Asp Asp Glu Thr Leu 225 230 235 240 Ile Arg Leu Ala Glu Leu Trp Lys Asp Pro Ala Val Thr Ala Ala Val 245 250 255 Leu Glu Thr Ser Gly Asn Thr Ala Val Thr Val Glu Arg Ser Pro Glu 260 265 270 Glu Leu Ala Ala Ile Met Glu Arg Leu Ala Ala Arg Glu Gln 275 280 285 <210> 11 <211> 2259 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1959) <223> gene metP <400> 11 tcaatcgtcg cgaaggggta gttcgcggcg agaacatcgt ttcgggtcag ggcattgaag 60 agggtggact tgccgacgtt gggcagaccg acgattccaa gagtaaggct cacgggcgta 120 gatcctagca gccagcggtt ttgcctctcc ggtcagcgcc cgtacctgtg ttgcgggatg 180 atacggacgt cattatatga tcaggccact tcaacgataa atacgtcgca ccctggacgt 240 tttcctctcc ctaaccatcg ggagccaact acacatcctc cacctcagac aggtatacac 300 atg tct tcg acc acc ccg cag ccc gcg ccg tcg ggc cag gag cag ggc 348 Met Ser Ser Thr Thr Pro Gln Pro Ala Pro Ser Gly Gln Glu Gln Gly 1 5 10 15 gag cgg cgc gag atc ttc tcg tcc cgt tgg gcg ttc ctg ctg gcc gcc 396 Glu Arg Arg Glu Ile Phe Ser Ser Arg Trp Ala Phe Leu Leu Ala Ala 20 25 30 atc ggc tcc gcc gtc ggc ctg ggc aac atc tgg cgc ttc ccc tac gtc 444 Ile Gly Ser Ala Val Gly Leu Gly Asn Ile Trp Arg Phe Pro Tyr Val 35 40 45 gct tac gac aac ggc ggc ggc gcc ttc ctc atc ccc tac ctc gtg gca 492 Ala Tyr Asp Asn Gly Gly Gly Ala Phe Leu Ile Pro Tyr Leu Val Ala 50 55 60 ctg ctg tcc gct ggt atc ccg ctg ctc ttc ctc gac tac gcc ctg ggc 540 Leu Leu Ser Ala Gly Ile Pro Leu Leu Phe Leu Asp Tyr Ala Leu Gly 65 70 75 80 cac cgc tac cgt ggt tcc gcc ccg ctg gtg ttc cgt cgc atc aag tcc 588 His Arg Tyr Arg Gly Ser Ala Pro Leu Val Phe Arg Arg Ile Lys Ser 85 90 95 tgg gct gag ccg gtc ggc tgg gtc cag gtc ggt gtg tcc ttt ttc atc 636 Trp Ala Glu Pro Val Gly Trp Val Gln Val Gly Val Ser Phe Phe Ile 100 105 110 act atc tac tac gcc gcg atc atc ggc tgg gcc gcg ctc tac gcg gtc 684 Thr Ile Tyr Tyr Ala Ala Ile Ile Gly Trp Ala Ala Leu Tyr Ala Val 115 120 125 aag tcc ttc aac aag gcg tgg ggc acc gac ccg aac agc tac ttc ttc 732 Lys Ser Phe Asn Lys Ala Trp Gly Thr Asp Pro Asn Ser Tyr Phe Phe 130 135 140 ggc gat ttc ctc cag ttc gac gag acc gcc acc ttc tcc acc gac ttc 780 Gly Asp Phe Leu Gln Phe Asp Glu Thr Ala Thr Phe Ser Thr Asp Phe 145 150 155 160 gtc ccg cag atc gcc ctg gcc ctg gcc ggc gtg tgg atc gcc gcg atc 828 Val Pro Gln Ile Ala Leu Ala Leu Ala Gly Val Trp Ile Ala Ala Ile 165 170 175 atc gtg ctg gcg atc ggc gtg gac aag ggc atc ggc aag gtc tcc aag 876 Ile Val Leu Ala Ile Gly Val Asp Lys Gly Ile Gly Lys Val Ser Lys 180 185 190 atc ttc atc ccc ctg ctg gcg gtc ctc ttc ctc atc gtc gta gtc cag 924 Ile Phe Ile Pro Leu Leu Ala Val Leu Phe Leu Ile Val Val Val Gln 195 200 205 gcg ctc atg ctg ccg ggt gcc gcc acg ggt ctc gac gcc ctg ttc acc 972 Ala Leu Met Leu Pro Gly Ala Ala Thr Gly Leu Asp Ala Leu Phe Thr 210 215 220 ccg gac tgg aac gcc ctg acc aac ggc act gtg tgg atc gcc gcc tac 1020 Pro Asp Trp Asn Ala Leu Thr Asn Gly Thr Val Trp Ile Ala Ala Tyr 225 230 235 240 ggc cag atc ttc ttc tcc ctg tcc gtg agc ttc ggc atc atg ctc acc 1068 Gly Gln Ile Phe Phe Ser Leu Ser Val Ser Phe Gly Ile Met Leu Thr 245 250 255 tac tcc tcc tac ctg cgt ccg cgc acc aac ctg acg ggc acc ggc ctg 1116 Tyr Ser Ser Tyr Leu Arg Pro Arg Thr Asn Leu Thr Gly Thr Gly Leu 260 265 270 acc acc gcc ttc gcg aac tcc tcc ttc gag gtc ctc gcc ggc gtc ggt 1164 Thr Thr Ala Phe Ala Asn Ser Ser Phe Glu Val Leu Ala Gly Val Gly 275 280 285 gtc ttc gcc gcc ctg ggc ttc atg gcc gtg cag cag tcg gtc gcc gtc 1212 Val Phe Ala Ala Leu Gly Phe Met Ala Val Gln Gln Ser Val Ala Val 290 295 300 gac gag gtc gcg acc tcg ggc atc ggt ctg gcc ttc gtg gcc ttc ccg 1260 Asp Glu Val Ala Thr Ser Gly Ile Gly Leu Ala Phe Val Ala Phe Pro 305 310 315 320 gcg atc atc aac gag atg gcc ttc ggc ccg atc ttc ggc gtc ctg ttc 1308 Ala Ile Ile Asn Glu Met Ala Phe Gly Pro Ile Phe Gly Val Leu Phe 325 330 335 ttc ggc tcc ctc acc atc gcc ggc ttc acc tcg ctg ttc tcc ctg ctc 1356 Phe Gly Ser Leu Thr Ile Ala Gly Phe Thr Ser Leu Phe Ser Leu Leu 340 345 350 gag gtc gtc gtc tcc gcc gtc aag gac aag ctg aac ctg ccg cgc aag 1404 Glu Val Val Val Ser Ala Val Lys Asp Lys Leu Asn Leu Pro Arg Lys 355 360 365 acc gtc gcc atc ggc atc ggc acc ttc atg gcg ctg atc tcc ctg gcg 1452 Thr Val Ala Ile Gly Ile Gly Thr Phe Met Ala Leu Ile Ser Leu Ala 370 375 380 ctg ttc tcc acg acg tcc ggc ctc atg gcc ctg gac atc atg gac aag 1500 Leu Phe Ser Thr Thr Ser Gly Leu Met Ala Leu Asp Ile Met Asp Lys 385 390 395 400 ttc acc aac aac atc ggc atc gtc gcc atc gcc ctg atc gcg gtc atc 1548 Phe Thr Asn Asn Ile Gly Ile Val Ala Ile Ala Leu Ile Ala Val Ile 405 410 415 atc ctc gat tgg gtc ctg cgc cgg atc gac gag ttc tcg ctc cac atc 1596 Ile Leu Asp Trp Val Leu Arg Arg Ile Asp Glu Phe Ser Leu His Ile 420 425 430 aac gcc gtg tcc tcc ttc aag gtg ggc acg ctg tgg cgc atc ttc gtg 1644 Asn Ala Val Ser Ser Phe Lys Val Gly Thr Leu Trp Arg Ile Phe Val 435 440 445 gtc aac gtc acc acc ctg gtg ctg gga ttc acc ctg gcg cag gag ctc 1692 Val Asn Val Thr Thr Leu Val Leu Gly Phe Thr Leu Ala Gln Glu Leu 450 455 460 atc tcc ctg atc cag gag ccc tac ggc ggc tac acg acg acg cag atc 1740 Ile Ser Leu Ile Gln Glu Pro Tyr Gly Gly Tyr Thr Thr Thr Gln Ile 465 470 475 480 gcc atc tac ggc tgg gcc gtc ctg gcg ctc atc ggt atc aac gcc ctc 1788 Ala Ile Tyr Gly Trp Ala Val Leu Ala Leu Ile Gly Ile Asn Ala Leu 485 490 495 gtc ttc tcc ctg gtg ccg tgg cgc ggc aac ctg ccg ctc aac ggt ccg 1836 Val Phe Ser Leu Val Pro Trp Arg Gly Asn Leu Pro Leu Asn Gly Pro 500 505 510 ccg ggc tcc gac ttc ggt gtt gac ccg gag gtc cac cgc gag ctc cac 1884 Pro Gly Ser Asp Phe Gly Val Asp Pro Glu Val His Arg Glu Leu His 515 520 525 gcg ccg cgc aag tac aac ggt gac cac cgg gtg ggc gtc gac aag cac 1932 Ala Pro Arg Lys Tyr Asn Gly Asp His Arg Val Gly Val Asp Lys His 530 535 540 acc acc gag gaa gga acg atc cga tga gcggtctcgc aatcatgatg 1979 Thr Thr Glu Glu Gly Thr Ile Arg 545 550 atggtcctgt tcatggtcgt catctggggt ggtttcatcg ccagcgccct gcacctgcgg 2039 gcgaaccccg atgacacctc cggcgccctg ggcgtcgcgg accatgcgcg cgacgagcac 2099 ctcgccgcgc aggaactcca ctagtcacac cacaccggga tgtcaggcaa tatggagtct 2159 gtgactgacc agccgacctc ccgcatcccc ggggacgaga ctcccccgac cggctcctcc 2219 ggcgacgccg ggtccaccgc cttcggcagc atggacctgg 2259 <210> 12 <211> 552 <212> PRT <213> Corynebacterium humireducens <400> 12 Met Ser Ser Thr Thr Pro Gln Pro Ala Pro Ser Gly Gln Glu Gln Gly 1 5 10 15 Glu Arg Arg Glu Ile Phe Ser Ser Arg Trp Ala Phe Leu Leu Ala Ala 20 25 30 Ile Gly Ser Ala Val Gly Leu Gly Asn Ile Trp Arg Phe Pro Tyr Val 35 40 45 Ala Tyr Asp Asn Gly Gly Gly Ala Phe Leu Ile Pro Tyr Leu Val Ala 50 55 60 Leu Leu Ser Ala Gly Ile Pro Leu Leu Phe Leu Asp Tyr Ala Leu Gly 65 70 75 80 His Arg Tyr Arg Gly Ser Ala Pro Leu Val Phe Arg Arg Ile Lys Ser 85 90 95 Trp Ala Glu Pro Val Gly Trp Val Gln Val Gly Val Ser Phe Phe Ile 100 105 110 Thr Ile Tyr Tyr Ala Ala Ile Ile Gly Trp Ala Ala Leu Tyr Ala Val 115 120 125 Lys Ser Phe Asn Lys Ala Trp Gly Thr Asp Pro Asn Ser Tyr Phe Phe 130 135 140 Gly Asp Phe Leu Gln Phe Asp Glu Thr Ala Thr Phe Ser Thr Asp Phe 145 150 155 160 Val Pro Gln Ile Ala Leu Ala Leu Ala Gly Val Trp Ile Ala Ala Ile 165 170 175 Ile Val Leu Ala Ile Gly Val Asp Lys Gly Ile Gly Lys Val Ser Lys 180 185 190 Ile Phe Ile Pro Leu Leu Ala Val Leu Phe Leu Ile Val Val Val Gln 195 200 205 Ala Leu Met Leu Pro Gly Ala Ala Thr Gly Leu Asp Ala Leu Phe Thr 210 215 220 Pro Asp Trp Asn Ala Leu Thr Asn Gly Thr Val Trp Ile Ala Ala Tyr 225 230 235 240 Gly Gln Ile Phe Phe Ser Leu Ser Val Ser Phe Gly Ile Met Leu Thr 245 250 255 Tyr Ser Ser Tyr Leu Arg Pro Arg Thr Asn Leu Thr Gly Thr Gly Leu 260 265 270 Thr Thr Ala Phe Ala Asn Ser Ser Phe Glu Val Leu Ala Gly Val Gly 275 280 285 Val Phe Ala Ala Leu Gly Phe Met Ala Val Gln Gln Ser Val Ala Val 290 295 300 Asp Glu Val Ala Thr Ser Gly Ile Gly Leu Ala Phe Val Ala Phe Pro 305 310 315 320 Ala Ile Ile Asn Glu Met Ala Phe Gly Pro Ile Phe Gly Val Leu Phe 325 330 335 Phe Gly Ser Leu Thr Ile Ala Gly Phe Thr Ser Leu Phe Ser Leu Leu 340 345 350 Glu Val Val Val Ser Ala Val Lys Asp Lys Leu Asn Leu Pro Arg Lys 355 360 365 Thr Val Ala Ile Gly Ile Gly Thr Phe Met Ala Leu Ile Ser Leu Ala 370 375 380 Leu Phe Ser Thr Thr Ser Gly Leu Met Ala Leu Asp Ile Met Asp Lys 385 390 395 400 Phe Thr Asn Asn Ile Gly Ile Val Ala Ile Ala Leu Ile Ala Val Ile 405 410 415 Ile Leu Asp Trp Val Leu Arg Arg Ile Asp Glu Phe Ser Leu His Ile 420 425 430 Asn Ala Val Ser Ser Phe Lys Val Gly Thr Leu Trp Arg Ile Phe Val 435 440 445 Val Asn Val Thr Thr Leu Val Leu Gly Phe Thr Leu Ala Gln Glu Leu 450 455 460 Ile Ser Leu Ile Gln Glu Pro Tyr Gly Gly Tyr Thr Thr Thr Gln Ile 465 470 475 480 Ala Ile Tyr Gly Trp Ala Val Leu Ala Leu Ile Gly Ile Asn Ala Leu 485 490 495 Val Phe Ser Leu Val Pro Trp Arg Gly Asn Leu Pro Leu Asn Gly Pro 500 505 510 Pro Gly Ser Asp Phe Gly Val Asp Pro Glu Val His Arg Glu Leu His 515 520 525 Ala Pro Arg Lys Tyr Asn Gly Asp His Arg Val Gly Val Asp Lys His 530 535 540 Thr Thr Glu Glu Gly Thr Ile Arg 545 550 <210> 13 <211> 1650 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1350) <223> gene metN <400> 13 ccgtacatca acgcctgggt gaccacccgc gacaaggccg acgacgagac cctcatccgt 60 ctcgccgagc tgtggaagga cccggccgtc accgccgccg tcctggagac ctccggcaac 120 accgccgtca ccgtcgagcg ctcgcccgag gagctcgccg cgatcatgga gcgcctcgcg 180 gcccgcgagc agtaggccgc actccgccca cctgccccgt cgcgttcccg cggcggggtt 240 tagcatttac ctgaccagtc cccctcccag aacggccccc agaacaccgt gaccgaacct 300 gtg acc gca cca ccc acc cgg ccg cgt acc ggt acc cgc atc gag ttc 348 Val Thr Ala Pro Pro Thr Arg Pro Arg Thr Gly Thr Arg Ile Glu Phe 1 5 10 15 cgc gat gtg acc aag gtc ttc acc aac cag cgg aag acc gag acc cgc 396 Arg Asp Val Thr Lys Val Phe Thr Asn Gln Arg Lys Thr Glu Thr Arg 20 25 30 gcc gtc gac ggc gtc acc ctg acc gtc gaa ccc ggc gag atc ctc ggc 444 Ala Val Asp Gly Val Thr Leu Thr Val Glu Pro Gly Glu Ile Leu Gly 35 40 45 gtc atc ggc tac tcc ggc gcc ggc aag tcg acg ctc gtc cgc ctc atc 492 Val Ile Gly Tyr Ser Gly Ala Gly Lys Ser Thr Leu Val Arg Leu Ile 50 55 60 aac ggg ctg gac tcc aca acc tcc ggc cag ctg ctt ctc gac ggc acc 540 Asn Gly Leu Asp Ser Thr Thr Ser Gly Gln Leu Leu Leu Asp Gly Thr 65 70 75 80 gac atc gtc gga ctc ccc gag tcc aag ctg cgc tcg atc cgc cgg ggc 588 Asp Ile Val Gly Leu Pro Glu Ser Lys Leu Arg Ser Ile Arg Arg Gly 85 90 95 atc ggc atg atc ttc cag cag ttc aac ctc ttc cag tcc cgc acg gcc 636 Ile Gly Met Ile Phe Gln Gln Phe Asn Leu Phe Gln Ser Arg Thr Ala 100 105 110 gcc ggc aac atc gcc tac ccg ctg gag ctg gcc ggc atg ccg cgc gcg 684 Ala Gly Asn Ile Ala Tyr Pro Leu Glu Leu Ala Gly Met Pro Arg Ala 115 120 125 gag cgg cgg cgg cgc gtc gcc gag ctc ctc gac ttc gtc ggc ctg ggc 732 Glu Arg Arg Arg Arg Val Ala Glu Leu Leu Asp Phe Val Gly Leu Gly 130 135 140 gac aag ggc gac aac tac ccg gag cag ctg tcg ggc ggc cag aag cag 780 Asp Lys Gly Asp Asn Tyr Pro Glu Gln Leu Ser Gly Gly Gln Lys Gln 145 150 155 160 cgc gtc ggc atc gcc cgc gcc ctg gcc acc aac ccg cgg ctc ctg ctc 828 Arg Val Gly Ile Ala Arg Ala Leu Ala Thr Asn Pro Arg Leu Leu Leu 165 170 175 gcc gac gag gcc acc tcg gcc ctc gac ccg gag acc acc cag gac gtg 876 Ala Asp Glu Ala Thr Ser Ala Leu Asp Pro Glu Thr Thr Gln Asp Val 180 185 190 ctc gac ctg ctc cgg gag atc aac cgc gag ctg ggc atc acc atc gtc 924 Leu Asp Leu Leu Arg Glu Ile Asn Arg Glu Leu Gly Ile Thr Ile Val 195 200 205 gta atc acc cat gag atg gac gtc gtg cgt tcc atc gcc gac aag gtc 972 Val Ile Thr His Glu Met Asp Val Val Arg Ser Ile Ala Asp Lys Val 210 215 220 gcc gtc atg gag gcc ggc cgg gtg gtc gag cac ggc agc gtc tac gag 1020 Ala Val Met Glu Ala Gly Arg Val Val Glu His Gly Ser Val Tyr Glu 225 230 235 240 gtg ttc tcc cgc ccg cag act gcg gtg gcg cgg cgc ttc gtg tcc acc 1068 Val Phe Ser Arg Pro Gln Thr Ala Val Ala Arg Arg Phe Val Ser Thr 245 250 255 tcg ctg cgc aac acc ccc gac cag gtc gag gcg gag gac ctg ctg gcc 1116 Ser Leu Arg Asn Thr Pro Asp Gln Val Glu Ala Glu Asp Leu Leu Ala 260 265 270 cac gag ggc cgc ctg ttc acc atc acc ctc acg gag gac tcc ggg ttc 1164 His Glu Gly Arg Leu Phe Thr Ile Thr Leu Thr Glu Asp Ser Gly Phe 275 280 285 ttc gcc gcg gcc gcc gcc ggc cgt gag gag ggg atg ctc atc aac atc 1212 Phe Ala Ala Ala Ala Ala Gly Arg Glu Glu Gly Met Leu Ile Asn Ile 290 295 300 gtc cac ggc ggc gtc acc acg ctg cag cgg cag tcc ttc ggc aag atg 1260 Val His Gly Gly Val Thr Thr Leu Gln Arg Gln Ser Phe Gly Lys Met 305 310 315 320 acc gtc cgg gtg acg ggg ccg gac gcg gcc gtc gag aag ttc cgt gac 1308 Thr Val Arg Val Thr Gly Pro Asp Ala Ala Val Glu Lys Phe Arg Asp 325 330 335 acg ctg tcc cgc acc acc gac atc cag gag atc acg cga tga 1350 Thr Leu Ser Arg Thr Thr Asp Ile Gln Glu Ile Thr Arg 340 345 acgagaccct cctcgccgca gactggaacc gtctcggccc gaccttcatc gacgccatcg 1410 gcgacaccct cgtcatggtg tccctcacga tggtgctcgg cggcctcggc ggcctgctca 1470 tcggcctgct gctgtacacg tcccgcgcgg gcggcatcct gcagtcgaag ccgctgtact 1530 ggacgctcaa catcctggtg aacttcgtcc gcccgatccc cttcatcgtg ctcatcgcca 1590 tgctggcgcc ggtgacggtc caggtcgtgg gcaccaccat cggccggtcc gcggcgatct 1650 <210> 14 <211> 349 <212> PRT <213> Corynebacterium humireducens <400> 14 Val Thr Ala Pro Pro Thr Arg Pro Arg Thr Gly Thr Arg Ile Glu Phe 1 5 10 15 Arg Asp Val Thr Lys Val Phe Thr Asn Gln Arg Lys Thr Glu Thr Arg 20 25 30 Ala Val Asp Gly Val Thr Leu Thr Val Glu Pro Gly Glu Ile Leu Gly 35 40 45 Val Ile Gly Tyr Ser Gly Ala Gly Lys Ser Thr Leu Val Arg Leu Ile 50 55 60 Asn Gly Leu Asp Ser Thr Thr Ser Gly Gln Leu Leu Leu Asp Gly Thr 65 70 75 80 Asp Ile Val Gly Leu Pro Glu Ser Lys Leu Arg Ser Ile Arg Arg Gly 85 90 95 Ile Gly Met Ile Phe Gln Gln Phe Asn Leu Phe Gln Ser Arg Thr Ala 100 105 110 Ala Gly Asn Ile Ala Tyr Pro Leu Glu Leu Ala Gly Met Pro Arg Ala 115 120 125 Glu Arg Arg Arg Arg Val Ala Glu Leu Leu Asp Phe Val Gly Leu Gly 130 135 140 Asp Lys Gly Asp Asn Tyr Pro Glu Gln Leu Ser Gly Gly Gln Lys Gln 145 150 155 160 Arg Val Gly Ile Ala Arg Ala Leu Ala Thr Asn Pro Arg Leu Leu Leu 165 170 175 Ala Asp Glu Ala Thr Ser Ala Leu Asp Pro Glu Thr Thr Gln Asp Val 180 185 190 Leu Asp Leu Leu Arg Glu Ile Asn Arg Glu Leu Gly Ile Thr Ile Val 195 200 205 Val Ile Thr His Glu Met Asp Val Val Arg Ser Ile Ala Asp Lys Val 210 215 220 Ala Val Met Glu Ala Gly Arg Val Val Glu His Gly Ser Val Tyr Glu 225 230 235 240 Val Phe Ser Arg Pro Gln Thr Ala Val Ala Arg Arg Phe Val Ser Thr 245 250 255 Ser Leu Arg Asn Thr Pro Asp Gln Val Glu Ala Glu Asp Leu Leu Ala 260 265 270 His Glu Gly Arg Leu Phe Thr Ile Thr Leu Thr Glu Asp Ser Gly Phe 275 280 285 Phe Ala Ala Ala Ala Ala Gly Arg Glu Glu Gly Met Leu Ile Asn Ile 290 295 300 Val His Gly Gly Val Thr Thr Leu Gln Arg Gln Ser Phe Gly Lys Met 305 310 315 320 Thr Val Arg Val Thr Gly Pro Asp Ala Ala Val Glu Lys Phe Arg Asp 325 330 335 Thr Leu Ser Arg Thr Thr Asp Ile Gln Glu Ile Thr Arg 340 345 <210> 15 <211> 1854 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1554) <223> gene metK <400> 15 gacggacaga ccagcgcgct cgagtacgcg caggccaagc tcacccgcaa gggctgcgac 60 ctgctcatgg ccaacgaggt gggggagggc cgggtcttcg gccagacccg caacagcggc 120 tggctgctca ccgccgacgg cgctgtcacg gaggttccgg acggttccaa gcacgtggtg 180 gcggcggcga tcctcagcgc cgtcgacgac ctgcgcaatt gacgctttta gaccgcttgg 240 tctatattgg gcgggacgct cagtcatatc attaatttga cccccgaaaa ggacccgccc 300 gtg tca gag aac ccc gcc acc acc acg gtg gcc ttc gac aag ccc gcc 348 Val Ser Glu Asn Pro Ala Thr Thr Thr Val Ala Phe Asp Lys Pro Ala 1 5 10 15 acg atc cgc ctg ttc acc agc gag tcc gtc acc gag ggc cac ccg gac 396 Thr Ile Arg Leu Phe Thr Ser Glu Ser Val Thr Glu Gly His Pro Asp 20 25 30 aag atc tgc gac gcc atc tcg gac acc atc ctc gac gcc atg ctc gag 444 Lys Ile Cys Asp Ala Ile Ser Asp Thr Ile Leu Asp Ala Met Leu Glu 35 40 45 cag gac ccg acc gcg cgc gtg gcc gtc gag acg ctc gtg acc acc ggc 492 Gln Asp Pro Thr Ala Arg Val Ala Val Glu Thr Leu Val Thr Thr Gly 50 55 60 cag gtc cac gtc gtc ggt gag gtc cgc acc acc ggc tac gtc gag atc 540 Gln Val His Val Val Gly Glu Val Arg Thr Thr Gly Tyr Val Glu Ile 65 70 75 80 ccg cag ctc gtg cgc aag acc ctc gtg gac atc ggc ttc atc tcc tcc 588 Pro Gln Leu Val Arg Lys Thr Leu Val Asp Ile Gly Phe Ile Ser Ser 85 90 95 gag gtc ggc ttc gac ggc acc acc tgc ggc gtc aac atc gcc atc ggc 636 Glu Val Gly Phe Asp Gly Thr Thr Cys Gly Val Asn Ile Ala Ile Gly 100 105 110 gag cag tcc cag gag atc gcc gac ggc gtc gac tcc tcc cac gag gag 684 Glu Gln Ser Gln Glu Ile Ala Asp Gly Val Asp Ser Ser His Glu Glu 115 120 125 cgt tcc ggc gtc gag atc gac gag gac gac cgc gcc ggc gcc ggc gac 732 Arg Ser Gly Val Glu Ile Asp Glu Asp Asp Arg Ala Gly Ala Gly Asp 130 135 140 cag ggc ctc atg ttc ggc tac gcc agc aac gag acc ccc gag tac atg 780 Gln Gly Leu Met Phe Gly Tyr Ala Ser Asn Glu Thr Pro Glu Tyr Met 145 150 155 160 ccg ctg ccc atc gcc ctg gcc cac cgc ctg gcc cgc cgc ctc acc gag 828 Pro Leu Pro Ile Ala Leu Ala His Arg Leu Ala Arg Arg Leu Thr Glu 165 170 175 gtg cgc aac cag ggc atc gtc ccg cac ctg cgt ccc gac ggc aag acc 876 Val Arg Asn Gln Gly Ile Val Pro His Leu Arg Pro Asp Gly Lys Thr 180 185 190 cag gtc acc ttc gcc tac gac gag cag gac cgc ccg gtc cgc ctc gac 924 Gln Val Thr Phe Ala Tyr Asp Glu Gln Asp Arg Pro Val Arg Leu Asp 195 200 205 acc gtg gtc atc tcc acg cag cac gac ccg gag gtc acc cag gac tgg 972 Thr Val Val Ile Ser Thr Gln His Asp Pro Glu Val Thr Gln Asp Trp 210 215 220 ctg cac gag cag ctg cgc gag cac gtc ctc gac tgg gtc atc gcc gac 1020 Leu His Glu Gln Leu Arg Glu His Val Leu Asp Trp Val Ile Ala Asp 225 230 235 240 gcc ggc ctg atc ggc atg gtc gac gag gag ctc acc ctc ctg gtc aac 1068 Ala Gly Leu Ile Gly Met Val Asp Glu Glu Leu Thr Leu Leu Val Asn 245 250 255 ccg tcc ggc tcc ttc atc ctc ggc ggc ccg atg ggc gac gct ggc ctg 1116 Pro Ser Gly Ser Phe Ile Leu Gly Gly Pro Met Gly Asp Ala Gly Leu 260 265 270 acg ggc cgc aag atc atc gtc gac acc tac ggc ggc atg gcc cgc cac 1164 Thr Gly Arg Lys Ile Ile Val Asp Thr Tyr Gly Gly Met Ala Arg His 275 280 285 ggc ggc ggc gcc ttc tcc ggc aag gac ccg tcc aag gtg gac cgt tcc 1212 Gly Gly Gly Ala Phe Ser Gly Lys Asp Pro Ser Lys Val Asp Arg Ser 290 295 300 gcc gcg tac gcc atg cgc tgg gtg gcc aag aac atc gtc gcc gcc ggc 1260 Ala Ala Tyr Ala Met Arg Trp Val Ala Lys Asn Ile Val Ala Ala Gly 305 310 315 320 ctg gcc gag cgc gtc gag gtc cag gtg gcc tac gcc atc ggc cgc gcg 1308 Leu Ala Glu Arg Val Glu Val Gln Val Ala Tyr Ala Ile Gly Arg Ala 325 330 335 aag ccg gtc ggc ctc tac gtg gag tcc ttc ggc acc gcc gcc gcg ggc 1356 Lys Pro Val Gly Leu Tyr Val Glu Ser Phe Gly Thr Ala Ala Ala Gly 340 345 350 ctc agt gac gcg gac atc cag ggc gcc gtc gag aag gtc ttc gac ctg 1404 Leu Ser Asp Ala Asp Ile Gln Gly Ala Val Glu Lys Val Phe Asp Leu 355 360 365 cgg ccc gcc gcc atc atc cgc gag ctc gac ctg ctg cgc ccg atc tac 1452 Arg Pro Ala Ala Ile Ile Arg Glu Leu Asp Leu Leu Arg Pro Ile Tyr 370 375 380 gcg cag acc tcc gcc tac ggc cac ttc ggc cgc ctc gac ctg gat ctg 1500 Ala Gln Thr Ser Ala Tyr Gly His Phe Gly Arg Leu Asp Leu Asp Leu 385 390 395 400 ccc tgg gag cgg ctc aac cgg gtg gac gag ctc aag gcg gct ctg ggc 1548 Pro Trp Glu Arg Leu Asn Arg Val Asp Glu Leu Lys Ala Ala Leu Gly 405 410 415 cgc tag agtattcggc atggcctccc cccgcgtccc cgcagaacac ctgccggtgg 1604 Arg cgcgggttct gcctctcctg ggcctggccc acctcgaccg gcccttcgac taccaggtgc 1664 cgtcgacgct ggatgacgac gcccagcccg gcgtccgcgt ccgcatccgc ttcgccgggc 1724 ggcttgtcga cgccgtcctc ctcgaacgcc gcgccgacac cgagcacgag ggcgggctga 1784 gctggatcga gcgggtgatc tccccgcacg tcgtgtaccc gccgcgcacc gcccggctca 1844 tcgaggcgct 1854 <210> 16 <211> 417 <212> PRT <213> Corynebacterium humireducens <400> 16 Val Ser Glu Asn Pro Ala Thr Thr Thr Val Ala Phe Asp Lys Pro Ala 1 5 10 15 Thr Ile Arg Leu Phe Thr Ser Glu Ser Val Thr Glu Gly His Pro Asp 20 25 30 Lys Ile Cys Asp Ala Ile Ser Asp Thr Ile Leu Asp Ala Met Leu Glu 35 40 45 Gln Asp Pro Thr Ala Arg Val Ala Val Glu Thr Leu Val Thr Thr Gly 50 55 60 Gln Val His Val Val Gly Glu Val Arg Thr Thr Gly Tyr Val Glu Ile 65 70 75 80 Pro Gln Leu Val Arg Lys Thr Leu Val Asp Ile Gly Phe Ile Ser Ser 85 90 95 Glu Val Gly Phe Asp Gly Thr Thr Cys Gly Val Asn Ile Ala Ile Gly 100 105 110 Glu Gln Ser Gln Glu Ile Ala Asp Gly Val Asp Ser Ser His Glu Glu 115 120 125 Arg Ser Gly Val Glu Ile Asp Glu Asp Asp Arg Ala Gly Ala Gly Asp 130 135 140 Gln Gly Leu Met Phe Gly Tyr Ala Ser Asn Glu Thr Pro Glu Tyr Met 145 150 155 160 Pro Leu Pro Ile Ala Leu Ala His Arg Leu Ala Arg Arg Leu Thr Glu 165 170 175 Val Arg Asn Gln Gly Ile Val Pro His Leu Arg Pro Asp Gly Lys Thr 180 185 190 Gln Val Thr Phe Ala Tyr Asp Glu Gln Asp Arg Pro Val Arg Leu Asp 195 200 205 Thr Val Val Ile Ser Thr Gln His Asp Pro Glu Val Thr Gln Asp Trp 210 215 220 Leu His Glu Gln Leu Arg Glu His Val Leu Asp Trp Val Ile Ala Asp 225 230 235 240 Ala Gly Leu Ile Gly Met Val Asp Glu Glu Leu Thr Leu Leu Val Asn 245 250 255 Pro Ser Gly Ser Phe Ile Leu Gly Gly Pro Met Gly Asp Ala Gly Leu 260 265 270 Thr Gly Arg Lys Ile Ile Val Asp Thr Tyr Gly Gly Met Ala Arg His 275 280 285 Gly Gly Gly Ala Phe Ser Gly Lys Asp Pro Ser Lys Val Asp Arg Ser 290 295 300 Ala Ala Tyr Ala Met Arg Trp Val Ala Lys Asn Ile Val Ala Ala Gly 305 310 315 320 Leu Ala Glu Arg Val Glu Val Gln Val Ala Tyr Ala Ile Gly Arg Ala 325 330 335 Lys Pro Val Gly Leu Tyr Val Glu Ser Phe Gly Thr Ala Ala Ala Gly 340 345 350 Leu Ser Asp Ala Asp Ile Gln Gly Ala Val Glu Lys Val Phe Asp Leu 355 360 365 Arg Pro Ala Ala Ile Ile Arg Glu Leu Asp Leu Leu Arg Pro Ile Tyr 370 375 380 Ala Gln Thr Ser Ala Tyr Gly His Phe Gly Arg Leu Asp Leu Asp Leu 385 390 395 400 Pro Trp Glu Arg Leu Asn Arg Val Asp Glu Leu Lys Ala Ala Leu Gly 405 410 415 Arg <210> 17 <211> 1278 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(978) <223> gene metI <400> 17 ggcgcggcgc ttcgtgtcca cctcgctgcg caacaccccc gaccaggtcg aggcggagga 60 cctgctggcc cacgagggcc gcctgttcac catcaccctc acggaggact ccgggttctt 120 cgccgcggcc gccgccggcc gtgaggaggg gatgctcatc aacatcgtcc acggcggcgt 180 caccacgctg cagcggcagt ccttcggcaa gatgaccgtc cgggtgacgg ggccggacgc 240 ggccgtcgag aagttccgtg acacgctgtc ccgcaccacc gacatccagg agatcacgcg 300 atg aac gag acc ctc ctc gcc gca gac tgg aac cgt ctc ggc ccg acc 348 Met Asn Glu Thr Leu Leu Ala Ala Asp Trp Asn Arg Leu Gly Pro Thr 1 5 10 15 ttc atc gac gcc atc ggc gac acc ctc gtc atg gtg tcc ctc acg atg 396 Phe Ile Asp Ala Ile Gly Asp Thr Leu Val Met Val Ser Leu Thr Met 20 25 30 gtg ctc ggc ggc ctc ggc ggc ctg ctc atc ggc ctg ctg ctg tac acg 444 Val Leu Gly Gly Leu Gly Gly Leu Leu Ile Gly Leu Leu Leu Tyr Thr 35 40 45 tcc cgc gcg ggc ggc atc ctg cag tcg aag ccg ctg tac tgg acg ctc 492 Ser Arg Ala Gly Gly Ile Leu Gln Ser Lys Pro Leu Tyr Trp Thr Leu 50 55 60 aac atc ctg gtg aac ttc gtc cgc ccg atc ccc ttc atc gtg ctc atc 540 Asn Ile Leu Val Asn Phe Val Arg Pro Ile Pro Phe Ile Val Leu Ile 65 70 75 80 gcc atg ctg gcg ccg gtg acg gtc cag gtc gtg ggc acc acc atc ggc 588 Ala Met Leu Ala Pro Val Thr Val Gln Val Val Gly Thr Thr Ile Gly 85 90 95 cgg tcc gcg gcg atc ttc gtc atg tgc atc gcc gcc gcc ttc gcc gtg 636 Arg Ser Ala Ala Ile Phe Val Met Cys Ile Ala Ala Ala Phe Ala Val 100 105 110 gcc cgc atc gtc gag cag aac ctc gtg gcc atc gac ccc ggc gtc atc 684 Ala Arg Ile Val Glu Gln Asn Leu Val Ala Ile Asp Pro Gly Val Ile 115 120 125 gag gcc gcc cgc gcc atg ggg gcg ggg ccg tgg cgg atc atc cgc acg 732 Glu Ala Ala Arg Ala Met Gly Ala Gly Pro Trp Arg Ile Ile Arg Thr 130 135 140 gtg atc atc ccg gag gcc ctc ggc ccg ctg gtc ctc ggc tac acg ttc 780 Val Ile Ile Pro Glu Ala Leu Gly Pro Leu Val Leu Gly Tyr Thr Phe 145 150 155 160 atc ttc atc gcg gtg gtc gac atg tcc gcg atg gcc ggc tac atc ggc 828 Ile Phe Ile Ala Val Val Asp Met Ser Ala Met Ala Gly Tyr Ile Gly 165 170 175 ggt ggt ggc ctg ggt gac ttc gcc atc gtc tac ggc tac cgc gcc tac 876 Gly Gly Gly Leu Gly Asp Phe Ala Ile Val Tyr Gly Tyr Arg Ala Tyr 180 185 190 gac ctg gag gtc acg tac gcg gcg gtc atc gtc atc gtc atc atc gtg 924 Asp Leu Glu Val Thr Tyr Ala Ala Val Ile Val Ile Val Ile Ile Val 195 200 205 cag atc gcg cag ctg ctg ggc aac tgg ctc tcg cga aag ctc atg cgg 972 Gln Ile Ala Gln Leu Leu Gly Asn Trp Leu Ser Arg Lys Leu Met Arg 210 215 220 cgt tag cgtcgcccgc cgttcacacc cccgaccccg tgtcgggggt tttgcgtacc 1028 Arg 225 cggagccgaa acgggggctc ccgatcgaga acacgtcggg gcgacgacct gccgtgcgga 1088 agtacccttc tgcccgctcg accgcactgt ttccccagga cacaacctgg cctgcagaca 1148 cagacgggta cctcagaacg cagctagcct ggatgtcatg aagatcgccg ccttcgacat 1208 cgacgacacc ctgctgttcc cccacggcat cgccgaggag gacatccgcg ccatccgcgc 1268 ctggcaggag 1278 <210> 18 <211> 225 <212> PRT <213> Corynebacterium humireducens <400> 18 Met Asn Glu Thr Leu Leu Ala Ala Asp Trp Asn Arg Leu Gly Pro Thr 1 5 10 15 Phe Ile Asp Ala Ile Gly Asp Thr Leu Val Met Val Ser Leu Thr Met 20 25 30 Val Leu Gly Gly Leu Gly Gly Leu Leu Ile Gly Leu Leu Leu Tyr Thr 35 40 45 Ser Arg Ala Gly Gly Ile Leu Gln Ser Lys Pro Leu Tyr Trp Thr Leu 50 55 60 Asn Ile Leu Val Asn Phe Val Arg Pro Ile Pro Phe Ile Val Leu Ile 65 70 75 80 Ala Met Leu Ala Pro Val Thr Val Gln Val Val Gly Thr Thr Ile Gly 85 90 95 Arg Ser Ala Ala Ile Phe Val Met Cys Ile Ala Ala Ala Phe Ala Val 100 105 110 Ala Arg Ile Val Glu Gln Asn Leu Val Ala Ile Asp Pro Gly Val Ile 115 120 125 Glu Ala Ala Arg Ala Met Gly Ala Gly Pro Trp Arg Ile Ile Arg Thr 130 135 140 Val Ile Ile Pro Glu Ala Leu Gly Pro Leu Val Leu Gly Tyr Thr Phe 145 150 155 160 Ile Phe Ile Ala Val Val Asp Met Ser Ala Met Ala Gly Tyr Ile Gly 165 170 175 Gly Gly Gly Leu Gly Asp Phe Ala Ile Val Tyr Gly Tyr Arg Ala Tyr 180 185 190 Asp Leu Glu Val Thr Tyr Ala Ala Val Ile Val Ile Val Ile Ile Val 195 200 205 Gln Ile Ala Gln Leu Leu Gly Asn Trp Leu Ser Arg Lys Leu Met Arg 210 215 220 Arg 225 <210> 19 <211> 1509 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1209) <223> gene dapA <400> 19 ggcaggccgc ccgcgcggtg ctgccgaacg ccacggagtc acgcatcgtc gtcaccggca 60 actaccgggc gtggcgccac ttcatcggcg cccgggccac cgagcacgcg gacgtcgaga 120 tccgggcact ggcggtggcg tgcctccgcc tcctcgcgca gcagtccccg gtgctcttcg 180 ccgatttcca gatctccact ctctccgacg gcacagagat ggccgtcagt ccctacgcca 240 ccgacttctg accagcgagg gattccccag cggaaaggta aacaggtaac cttgaacacc 300 atg agt aca ggt atg act tcg aag acc gga gtc gag gac ttc ggt acc 348 Met Ser Thr Gly Met Thr Ser Lys Thr Gly Val Glu Asp Phe Gly Thr 1 5 10 15 gtc tgc gtc gcc atg gtc acg ccc ttc gac cgg gac ggc gcc ctg gac 396 Val Cys Val Ala Met Val Thr Pro Phe Asp Arg Asp Gly Ala Leu Asp 20 25 30 ctc gag gcc ggt cgc cgc atc gcg gcc cac ctc gtc gac aac ggt ctg 444 Leu Glu Ala Gly Arg Arg Ile Ala Ala His Leu Val Asp Asn Gly Leu 35 40 45 gac gcc ctc gtg ctc gca ggc acg acc ggt gag tcc ccg acc acc acg 492 Asp Ala Leu Val Leu Ala Gly Thr Thr Gly Glu Ser Pro Thr Thr Thr 50 55 60 ctc gac gag aag atc agc ctg ctc aag gcg gtg aag gag gag gtc ggc 540 Leu Asp Glu Lys Ile Ser Leu Leu Lys Ala Val Lys Glu Glu Val Gly 65 70 75 80 gac cgc tgc aag ctc atc gcg ggt gcc ggc acc aac gac acc gcc tcc 588 Asp Arg Cys Lys Leu Ile Ala Gly Ala Gly Thr Asn Asp Thr Ala Ser 85 90 95 tcc atc gag ctc gcc cgc gcc tcc gcc gag gcc ggc gcc gac gcc ctg 636 Ser Ile Glu Leu Ala Arg Ala Ser Ala Glu Ala Gly Ala Asp Ala Leu 100 105 110 ctc gtg gtc acc ccc tac tac tcg aag ccg agc cag gag ggt gtc tac 684 Leu Val Val Thr Pro Tyr Tyr Ser Lys Pro Ser Gln Glu Gly Val Tyr 115 120 125 cag cac ttc aag gcc gtc gcc ggg gcc acc gac ctg ccc atc atg ctc 732 Gln His Phe Lys Ala Val Ala Gly Ala Thr Asp Leu Pro Ile Met Leu 130 135 140 tac gac atc ccg ccg cgc tcc tcg atc ccg atc gag ggg gac acc atc 780 Tyr Asp Ile Pro Pro Arg Ser Ser Ile Pro Ile Glu Gly Asp Thr Ile 145 150 155 160 cgc cga ctc gcg gag ctg ccc acc att cgt gcg gtg aag gat gca aag 828 Arg Arg Leu Ala Glu Leu Pro Thr Ile Arg Ala Val Lys Asp Ala Lys 165 170 175 ggt aac atg gct gag tct gca ccg ctc atg cat gag acc ggt ctc gcc 876 Gly Asn Met Ala Glu Ser Ala Pro Leu Met His Glu Thr Gly Leu Ala 180 185 190 tgg tac tcg ggc gat gat ccg ctc aac gtc ccg tgg ctg tcg ctc ggc 924 Trp Tyr Ser Gly Asp Asp Pro Leu Asn Val Pro Trp Leu Ser Leu Gly 195 200 205 gcc tcg gga ttc gtt tcc gtg gtc ggc cat gcc gca ccc cag gca ctg 972 Ala Ser Gly Phe Val Ser Val Val Gly His Ala Ala Pro Gln Ala Leu 210 215 220 cgt gag ctg tac aca agt ttc gag gaa ggc gac ctc gcc cgt gcg cgg 1020 Arg Glu Leu Tyr Thr Ser Phe Glu Glu Gly Asp Leu Ala Arg Ala Arg 225 230 235 240 gaa atc aac gcc act gtt ctt tca ccg ctg atc gcc gct cag ggt cgc 1068 Glu Ile Asn Ala Thr Val Leu Ser Pro Leu Ile Ala Ala Gln Gly Arg 245 250 255 ctg ggt ggt gtc agc atg gcc aag gct gcc ctg cgt ctg cag ggc att 1116 Leu Gly Gly Val Ser Met Ala Lys Ala Ala Leu Arg Leu Gln Gly Ile 260 265 270 gag gtg ggg gac ccg cgt ctg cct gtc gtc gct ccc gac gag cag gag 1164 Glu Val Gly Asp Pro Arg Leu Pro Val Val Ala Pro Asp Glu Gln Glu 275 280 285 atc gag gcc ctc cgc cgt gac atg gaa aag gct gga gtc ctt taa 1209 Ile Glu Ala Leu Arg Arg Asp Met Glu Lys Ala Gly Val Leu 290 295 300 atatgactga atcccgtagt cgtgcccgga aggtcacccg caaggcgggc ccgccggaga 1269 ccgagagcac tggtgcatcc ccggttttcc aggcaccgac cccggaaacg aacggaaacg 1329 gcgcatccgc cgagaagaag accaccgaaa ggaagtccgc ggacaccgac aacgccccgc 1389 ggggcggcgg caaccgctcc cggaacggtg gcggccgcgc ccgcggcggc cgtggtggcc 1449 gcggcggtca gggtggccag ggccagggcg gtcagggtgg tcagcagggg ggacggggac 1509 <210> 20 <211> 302 <212> PRT <213> Corynebacterium humireducens <400> 20 Met Ser Thr Gly Met Thr Ser Lys Thr Gly Val Glu Asp Phe Gly Thr 1 5 10 15 Val Cys Val Ala Met Val Thr Pro Phe Asp Arg Asp Gly Ala Leu Asp 20 25 30 Leu Glu Ala Gly Arg Arg Ile Ala Ala His Leu Val Asp Asn Gly Leu 35 40 45 Asp Ala Leu Val Leu Ala Gly Thr Thr Gly Glu Ser Pro Thr Thr Thr 50 55 60 Leu Asp Glu Lys Ile Ser Leu Leu Lys Ala Val Lys Glu Glu Val Gly 65 70 75 80 Asp Arg Cys Lys Leu Ile Ala Gly Ala Gly Thr Asn Asp Thr Ala Ser 85 90 95 Ser Ile Glu Leu Ala Arg Ala Ser Ala Glu Ala Gly Ala Asp Ala Leu 100 105 110 Leu Val Val Thr Pro Tyr Tyr Ser Lys Pro Ser Gln Glu Gly Val Tyr 115 120 125 Gln His Phe Lys Ala Val Ala Gly Ala Thr Asp Leu Pro Ile Met Leu 130 135 140 Tyr Asp Ile Pro Pro Arg Ser Ser Ile Pro Ile Glu Gly Asp Thr Ile 145 150 155 160 Arg Arg Leu Ala Glu Leu Pro Thr Ile Arg Ala Val Lys Asp Ala Lys 165 170 175 Gly Asn Met Ala Glu Ser Ala Pro Leu Met His Glu Thr Gly Leu Ala 180 185 190 Trp Tyr Ser Gly Asp Asp Pro Leu Asn Val Pro Trp Leu Ser Leu Gly 195 200 205 Ala Ser Gly Phe Val Ser Val Val Gly His Ala Ala Pro Gln Ala Leu 210 215 220 Arg Glu Leu Tyr Thr Ser Phe Glu Glu Gly Asp Leu Ala Arg Ala Arg 225 230 235 240 Glu Ile Asn Ala Thr Val Leu Ser Pro Leu Ile Ala Ala Gln Gly Arg 245 250 255 Leu Gly Gly Val Ser Met Ala Lys Ala Ala Leu Arg Leu Gln Gly Ile 260 265 270 Glu Val Gly Asp Pro Arg Leu Pro Val Val Ala Pro Asp Glu Gln Glu 275 280 285 Ile Glu Ala Leu Arg Arg Asp Met Glu Lys Ala Gly Val Leu 290 295 300 <210> 21 <211> 1515 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1215) <223> CBS <400> 21 gggcctccgc gacgggagcg ctggaaccac cgcgccaggg gccctgtgcc tcggccacct 60 gagtggcgga cagggtgagg gcgacggcca gggcggccgc gctgatctga cggtgtcggc 120 gggtgaaagc catgttcatc atctcctatg agctctattg gcgtaagggt ggatgatagg 180 gcagaaaatg caggttagat accctgccgg gtatgggata tctcacgggc tgcgacggga 240 acttcgcggt ccggaaaaag ttcccgcctt cccttgactt tgcggcgtaa gatgtcattt 300 atg agt ctc gag ccc ggg aac acc ccg ctc gta cgt ctg gac cgg ctc 348 Met Ser Leu Glu Pro Gly Asn Thr Pro Leu Val Arg Leu Asp Arg Leu 1 5 10 15 ccc ggc cgg ccc ggt gtc acc ctg tgg gcg aag ctg gag ggc cac aac 396 Pro Gly Arg Pro Gly Val Thr Leu Trp Ala Lys Leu Glu Gly His Asn 20 25 30 ccc agc ggc agt gcc aag gac cgg acc gcg cac gcc atc ctc gcg gcg 444 Pro Ser Gly Ser Ala Lys Asp Arg Thr Ala His Ala Ile Leu Ala Ala 35 40 45 gcg gat ctg cgg ccc ggc gcg gtc atc gtc gag tcg agc tcg ggc aac 492 Ala Asp Leu Arg Pro Gly Ala Val Ile Val Glu Ser Ser Ser Gly Asn 50 55 60 ctc ggc gtc gcc ctg gcc cgg gag gcg gtc ctc ggc ggc tgg acc ttc 540 Leu Gly Val Ala Leu Ala Arg Glu Ala Val Leu Gly Gly Trp Thr Phe 65 70 75 80 cac tgt gtc gtc gat ccg aag gcg aat cgc tcg acc atc gcc acc atg 588 His Cys Val Val Asp Pro Lys Ala Asn Arg Ser Thr Ile Ala Thr Met 85 90 95 cgc gcg ttc ggg gcg gtc gtc cac gag gtc acc gca ccc gat ccg gcc 636 Arg Ala Phe Gly Ala Val Val His Glu Val Thr Ala Pro Asp Pro Ala 100 105 110 acc ggt gac tgg ctc gcc gcc cgg cgc gcg cgg gtc gcg gaa ctg ctc 684 Thr Gly Asp Trp Leu Ala Ala Arg Arg Ala Arg Val Ala Glu Leu Leu 115 120 125 acc gag atc ccc ggg gcc gtc tgc ctc gac cag tac tcc aac cgg gcg 732 Thr Glu Ile Pro Gly Ala Val Cys Leu Asp Gln Tyr Ser Asn Arg Ala 130 135 140 gcc ttc acg gcc cac gac gag ggc acg atg gcc gag atc gtc gcg cag 780 Ala Phe Thr Ala His Asp Glu Gly Thr Met Ala Glu Ile Val Ala Gln 145 150 155 160 ctc ggc cac acc ccc gcg cac ctg ttc gtg gcg gtg agc acc acc ggc 828 Leu Gly His Thr Pro Ala His Leu Phe Val Ala Val Ser Thr Thr Gly 165 170 175 acg atc ggc ggc tgc ctg cgc cgc atc gcc cgg gag ggg tgg gac acg 876 Thr Ile Gly Gly Cys Leu Arg Arg Ile Ala Arg Glu Gly Trp Asp Thr 180 185 190 gag gtc acc gcc gtc gac gcc gag ggt tcc gtg ctt ttc gac ggc gcc 924 Glu Val Thr Ala Val Asp Ala Glu Gly Ser Val Leu Phe Asp Gly Ala 195 200 205 cgt ggc cgc cgc cac ctt ccc ggc ttc ggc gcc ggc atc gtc ccg gac 972 Arg Gly Arg Arg His Leu Pro Gly Phe Gly Ala Gly Ile Val Pro Asp 210 215 220 ctg gcc gcc gag gtg acc ccg gac cgc atc gtc cgg gtc ggc gac cgt 1020 Leu Ala Ala Glu Val Thr Pro Asp Arg Ile Val Arg Val Gly Asp Arg 225 230 235 240 gac tcc gtc gcc gcc gcg cgg cag ctg gcc cgc acg gaa ggg gtg ttg 1068 Asp Ser Val Ala Ala Ala Arg Gln Leu Ala Arg Thr Glu Gly Val Leu 245 250 255 tgc ggt gcc tcg ggt ggg gcg gtg gtg gcg gcc gtc ggg aag ctc ctg 1116 Cys Gly Ala Ser Gly Gly Ala Val Val Ala Ala Val Gly Lys Leu Leu 260 265 270 ccg gag ctg cac ggg gac gtc gtc ctc gtg ctc cac gac ggt ggc acc 1164 Pro Glu Leu His Gly Asp Val Val Leu Val Leu His Asp Gly Gly Thr 275 280 285 cac tac ctg gac acc gtg tac aac gac gac tgg gtg gag gag aac ctg 1212 His Tyr Leu Asp Thr Val Tyr Asn Asp Asp Trp Val Glu Glu Asn Leu 290 295 300 tga caaccgtggt gatcatcggc ggcggcccgc gcggactgtg ggcggcggag 1265 gaactgctgg gcctggcacg cgagaggggc gcggccctcg acgtccacgt catcgacgac 1325 ggcggaccgc aggcctacga cccggggcag ccggaggagt ggctcatcaa cgtgcgggcc 1385 tccgtgatcc gcacggggct gggctccttc gacgagtggc gcggggccgc cgaccccttc 1445 ccgccgcgac gcgaggtggc ccgtttcctc gccgcctcct gggaggagct cgcacagcac 1505 gtgtcgccgc 1515 <210> 22 <211> 304 <212> PRT <213> Corynebacterium humireducens <400> 22 Met Ser Leu Glu Pro Gly Asn Thr Pro Leu Val Arg Leu Asp Arg Leu 1 5 10 15 Pro Gly Arg Pro Gly Val Thr Leu Trp Ala Lys Leu Glu Gly His Asn 20 25 30 Pro Ser Gly Ser Ala Lys Asp Arg Thr Ala His Ala Ile Leu Ala Ala 35 40 45 Ala Asp Leu Arg Pro Gly Ala Val Ile Val Glu Ser Ser Ser Gly Asn 50 55 60 Leu Gly Val Ala Leu Ala Arg Glu Ala Val Leu Gly Gly Trp Thr Phe 65 70 75 80 His Cys Val Val Asp Pro Lys Ala Asn Arg Ser Thr Ile Ala Thr Met 85 90 95 Arg Ala Phe Gly Ala Val Val His Glu Val Thr Ala Pro Asp Pro Ala 100 105 110 Thr Gly Asp Trp Leu Ala Ala Arg Arg Ala Arg Val Ala Glu Leu Leu 115 120 125 Thr Glu Ile Pro Gly Ala Val Cys Leu Asp Gln Tyr Ser Asn Arg Ala 130 135 140 Ala Phe Thr Ala His Asp Glu Gly Thr Met Ala Glu Ile Val Ala Gln 145 150 155 160 Leu Gly His Thr Pro Ala His Leu Phe Val Ala Val Ser Thr Thr Gly 165 170 175 Thr Ile Gly Gly Cys Leu Arg Arg Ile Ala Arg Glu Gly Trp Asp Thr 180 185 190 Glu Val Thr Ala Val Asp Ala Glu Gly Ser Val Leu Phe Asp Gly Ala 195 200 205 Arg Gly Arg Arg His Leu Pro Gly Phe Gly Ala Gly Ile Val Pro Asp 210 215 220 Leu Ala Ala Glu Val Thr Pro Asp Arg Ile Val Arg Val Gly Asp Arg 225 230 235 240 Asp Ser Val Ala Ala Ala Arg Gln Leu Ala Arg Thr Glu Gly Val Leu 245 250 255 Cys Gly Ala Ser Gly Gly Ala Val Val Ala Ala Val Gly Lys Leu Leu 260 265 270 Pro Glu Leu His Gly Asp Val Val Leu Val Leu His Asp Gly Gly Thr 275 280 285 His Tyr Leu Asp Thr Val Tyr Asn Asp Asp Trp Val Glu Glu Asn Leu 290 295 300 <210> 23 <211> 1746 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1446) <223> gene cg3031-homolog <400> 23 cccggagccg gagcgcctca acgtgctcaa caactcgacg gtgccgaacc tggccgcgca 60 ggtcgcggac cgcctgcggg aggacggttt cgaggtcggg gaggtcggca acctggcgga 120 ccacatcctc ccggagacca ccgtcttctt ccagcccggc aacgccgccg cggagcagcg 180 ggcgcgggag ctcgccgacc gcctcggcgg cgtcgcccgc gagtacgagg gcttcctccc 240 ggagaacacc gccggtcgca acgacctgac tctggtgctg gtgaaccagg tggcgctctg 300 atg ggc ccg ggg acc ggc atc gag ccg atc ccg ttc cgc cgc tcc ccg 348 Met Gly Pro Gly Thr Gly Ile Glu Pro Ile Pro Phe Arg Arg Ser Pro 1 5 10 15 cgg ccg acg ctc ggc gtc gag tgg gag atc gcg ctc atc gac ccc gag 396 Arg Pro Thr Leu Gly Val Glu Trp Glu Ile Ala Leu Ile Asp Pro Glu 20 25 30 acc cgc gac ctg gcg ccc aag gcc gtc gag gtc atc gac ctc gtc cgg 444 Thr Arg Asp Leu Ala Pro Lys Ala Val Glu Val Ile Asp Leu Val Arg 35 40 45 tcc cgc cac ccg gag gtg cac ctg gag aag gag ttc ctg cag aac acc 492 Ser Arg His Pro Glu Val His Leu Glu Lys Glu Phe Leu Gln Asn Thr 50 55 60 gtc gaa ctg gtc acc ggc atc cac gaa acc gtg ccg gag gcg gtc gcg 540 Val Glu Leu Val Thr Gly Ile His Glu Thr Val Pro Glu Ala Val Ala 65 70 75 80 gaa ctc gcc cgc gac ctg gcc gcc gtg aag gag gcc gcc gcc gac ctc 588 Glu Leu Ala Arg Asp Leu Ala Ala Val Lys Glu Ala Ala Ala Asp Leu 85 90 95 gga ctg tgc ctg tgg tcc tcc ggc tcg cac ccc ttc tcg gac tgg cgg 636 Gly Leu Cys Leu Trp Ser Ser Gly Ser His Pro Phe Ser Asp Trp Arg 100 105 110 aag aac ccg gtc agc gcg aag atg gcc tac gcc gag atc atc gag cgc 684 Lys Asn Pro Val Ser Ala Lys Met Ala Tyr Ala Glu Ile Ile Glu Arg 115 120 125 acc cgc tac tgg ggt tcg cag atg ctc atc tgg ggc atc cac gtg cac 732 Thr Arg Tyr Trp Gly Ser Gln Met Leu Ile Trp Gly Ile His Val His 130 135 140 gtc ggg atc agc cac gag gac cgg gtg tgg ccg atc atc aac gcc ctg 780 Val Gly Ile Ser His Glu Asp Arg Val Trp Pro Ile Ile Asn Ala Leu 145 150 155 160 atg acc cgc tac ccg cac ctg ctg gcg ctg acg gcg tcg tcg ccg ggc 828 Met Thr Arg Tyr Pro His Leu Leu Ala Leu Thr Ala Ser Ser Pro Gly 165 170 175 tgg gac ggc atc gac acg ggc tac gcc tcc aac cgc acg atg ctc tac 876 Trp Asp Gly Ile Asp Thr Gly Tyr Ala Ser Asn Arg Thr Met Leu Tyr 180 185 190 cag cag ctg ccc acc gcc ggc ctg ccc ttc cag ttc cgg aac tgg gcc 924 Gln Gln Leu Pro Thr Ala Gly Leu Pro Phe Gln Phe Arg Asn Trp Ala 195 200 205 gag tgg gag tcc tac atg cac gac cag ggc atc tcg ggc gtg atc aac 972 Glu Trp Glu Ser Tyr Met His Asp Gln Gly Ile Ser Gly Val Ile Asn 210 215 220 cac acc ggg tcg atg cac ttc gac atc cgt ccc gcc ccg aag tgg ggc 1020 His Thr Gly Ser Met His Phe Asp Ile Arg Pro Ala Pro Lys Trp Gly 225 230 235 240 acg gtc gag gtg cgg gtc tcc gac gcc acc tcc aac ctg cgg gaa ctc 1068 Thr Val Glu Val Arg Val Ser Asp Ala Thr Ser Asn Leu Arg Glu Leu 245 250 255 gcc gcc gtc gtg gcg ctc acg cac tgc ctg gtc gtc cac tac gac cgc 1116 Ala Ala Val Val Ala Leu Thr His Cys Leu Val Val His Tyr Asp Arg 260 265 270 atg atc gac cgc ggt gag gaa ctg ccc gtc ctg cag ccc tgg cac gtc 1164 Met Ile Asp Arg Gly Glu Glu Leu Pro Val Leu Gln Pro Trp His Val 275 280 285 gcc gag aac aag tgg cgg gcc gcc cgc tac ggg ctg gag gca ctc gtg 1212 Ala Glu Asn Lys Trp Arg Ala Ala Arg Tyr Gly Leu Glu Ala Leu Val 290 295 300 atc acc agc cgt gac acc gac gag cgc tgg gtc acc cag gag ctc gcg 1260 Ile Thr Ser Arg Asp Thr Asp Glu Arg Trp Val Thr Gln Glu Leu Ala 305 310 315 320 gag ctt gtc gac gaa ctc gcg ccc ctc gcc acc gaa ctc ggc tgc ctc 1308 Glu Leu Val Asp Glu Leu Ala Pro Leu Ala Thr Glu Leu Gly Cys Leu 325 330 335 gcc gaa ctc cag ctg gtc tcc gag atc ctc gaa cgt ggc gcc ggc tac 1356 Ala Glu Leu Gln Leu Val Ser Glu Ile Leu Glu Arg Gly Ala Gly Tyr 340 345 350 cag cgt cag cgt cgc atc cac gac gtc acc ggc agc tgg atc ccc gtc 1404 Gln Arg Gln Arg Arg Ile His Asp Val Thr Gly Ser Trp Ile Pro Val 355 360 365 atc gac gcc gcc tgc cgg gag atg gag gag atg gtc ccg tga 1446 Ile Asp Ala Ala Cys Arg Glu Met Glu Glu Met Val Pro 370 375 380 gcgacgtcgt cctcgtccac tccaccgggc acctgccgga cgtgtggacg gaggtggtgc 1506 gcgccttccc ggccgactgg aagccgtggc tgcccaatct gggcgtgcag tccctcgagc 1566 agtacctgga ccggcaggag ctgcgccggg tggtgctcgt cggtcacggc accggcgccc 1626 tggccgtggc acgcctggcc cgggagcagc cgcagcgggt cacccacgcg gtgctgtccg 1686 tgccggaggc gccccgcctc gtgccacgtt tcctgcggcg ccggcaggag gaggccctgg 1746 <210> 24 <211> 381 <212> PRT <213> Corynebacterium humireducens <400> 24 Met Gly Pro Gly Thr Gly Ile Glu Pro Ile Pro Phe Arg Arg Ser Pro 1 5 10 15 Arg Pro Thr Leu Gly Val Glu Trp Glu Ile Ala Leu Ile Asp Pro Glu 20 25 30 Thr Arg Asp Leu Ala Pro Lys Ala Val Glu Val Ile Asp Leu Val Arg 35 40 45 Ser Arg His Pro Glu Val His Leu Glu Lys Glu Phe Leu Gln Asn Thr 50 55 60 Val Glu Leu Val Thr Gly Ile His Glu Thr Val Pro Glu Ala Val Ala 65 70 75 80 Glu Leu Ala Arg Asp Leu Ala Ala Val Lys Glu Ala Ala Ala Asp Leu 85 90 95 Gly Leu Cys Leu Trp Ser Ser Gly Ser His Pro Phe Ser Asp Trp Arg 100 105 110 Lys Asn Pro Val Ser Ala Lys Met Ala Tyr Ala Glu Ile Ile Glu Arg 115 120 125 Thr Arg Tyr Trp Gly Ser Gln Met Leu Ile Trp Gly Ile His Val His 130 135 140 Val Gly Ile Ser His Glu Asp Arg Val Trp Pro Ile Ile Asn Ala Leu 145 150 155 160 Met Thr Arg Tyr Pro His Leu Leu Ala Leu Thr Ala Ser Ser Pro Gly 165 170 175 Trp Asp Gly Ile Asp Thr Gly Tyr Ala Ser Asn Arg Thr Met Leu Tyr 180 185 190 Gln Gln Leu Pro Thr Ala Gly Leu Pro Phe Gln Phe Arg Asn Trp Ala 195 200 205 Glu Trp Glu Ser Tyr Met His Asp Gln Gly Ile Ser Gly Val Ile Asn 210 215 220 His Thr Gly Ser Met His Phe Asp Ile Arg Pro Ala Pro Lys Trp Gly 225 230 235 240 Thr Val Glu Val Arg Val Ser Asp Ala Thr Ser Asn Leu Arg Glu Leu 245 250 255 Ala Ala Val Val Ala Leu Thr His Cys Leu Val Val His Tyr Asp Arg 260 265 270 Met Ile Asp Arg Gly Glu Glu Leu Pro Val Leu Gln Pro Trp His Val 275 280 285 Ala Glu Asn Lys Trp Arg Ala Ala Arg Tyr Gly Leu Glu Ala Leu Val 290 295 300 Ile Thr Ser Arg Asp Thr Asp Glu Arg Trp Val Thr Gln Glu Leu Ala 305 310 315 320 Glu Leu Val Asp Glu Leu Ala Pro Leu Ala Thr Glu Leu Gly Cys Leu 325 330 335 Ala Glu Leu Gln Leu Val Ser Glu Ile Leu Glu Arg Gly Ala Gly Tyr 340 345 350 Gln Arg Gln Arg Arg Ile His Asp Val Thr Gly Ser Trp Ile Pro Val 355 360 365 Ile Asp Ala Ala Cys Arg Glu Met Glu Glu Met Val Pro 370 375 380 <210> 25 <211> 1731 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1431) <223> gene aecD <400> 25 aggccgaacg cgccgtccag gccggcctgg acgagctgat gaaggaccgc accacgctca 60 tcatcgcgca ccgcctgtcg acgatcgcgg gtgtggacac catcgtcacc ctccgcgacg 120 gcatggtcga cgaaataggt tcaccggacc agctcgccgc ctcgggtggc atctacgccg 180 aactcctgca gctcacggcg tcctcctcgg ccgctgaccg ggcccgtctg aagaggtacg 240 ggctcgtcgt cgacggtcac ggcgacgagg aggacgagga agaggactag tctgtctggc 300 atg cag ttc ccc acc ctc gac cag ctc aag gac cgt cgc acc cgc aag 348 Met Gln Phe Pro Thr Leu Asp Gln Leu Lys Asp Arg Arg Thr Arg Lys 1 5 10 15 tgg acg gtc tac gcc gac gac gtc ctg ccc ctg tgg atc gcg gag agt 396 Trp Thr Val Tyr Ala Asp Asp Val Leu Pro Leu Trp Ile Ala Glu Ser 20 25 30 gac ttc ttc acc tgc ccg ccg gtg aag cag gcg atc gcg gac gcc atc 444 Asp Phe Phe Thr Cys Pro Pro Val Lys Gln Ala Ile Ala Asp Ala Ile 35 40 45 gac cgg gag tcc ttc ggg tac acc ccg gcg acc tcc gac ctg ccg cag 492 Asp Arg Glu Ser Phe Gly Tyr Thr Pro Ala Thr Ser Asp Leu Pro Gln 50 55 60 gcg gtc tcc gac ttc tac gcc gca cgt tac ggc tgg cgc ccg gac ccg 540 Ala Val Ser Asp Phe Tyr Ala Ala Arg Tyr Gly Trp Arg Pro Asp Pro 65 70 75 80 gcg ctc atc gtc gcg gtc ccc gac gtc gtc cgt ggc ctg gcc ctc gcc 588 Ala Leu Ile Val Ala Val Pro Asp Val Val Arg Gly Leu Ala Leu Ala 85 90 95 atc gag tac atg acg cgc ccg gac tcg ggt gtc atc gtg ccg gtg ccg 636 Ile Glu Tyr Met Thr Arg Pro Asp Ser Gly Val Ile Val Pro Val Pro 100 105 110 gcg tac ccg ccg ttc ctg gag ctg ccg gag gcg acc cgc cgt ccg atg 684 Ala Tyr Pro Pro Phe Leu Glu Leu Pro Glu Ala Thr Arg Arg Pro Met 115 120 125 cac cag atc gac gcc tac ggc ggg atc gac ctg ggg gac gtc gag aag 732 His Gln Ile Asp Ala Tyr Gly Gly Ile Asp Leu Gly Asp Val Glu Lys 130 135 140 cag ttc gcg gcc ggc gcc ggc tcg atc ctc ctg tgc gcc ccg aac aac 780 Gln Phe Ala Ala Gly Ala Gly Ser Ile Leu Leu Cys Ala Pro Asn Asn 145 150 155 160 ccc ctg ggg tac acg ttc gac gcg gag ttc ctg cgg gag ctg tgc gac 828 Pro Leu Gly Tyr Thr Phe Asp Ala Glu Phe Leu Arg Glu Leu Cys Asp 165 170 175 ctg gcg gac cgt tac gac gcc cgc gtg ctc gtc gac gag atc cac gcc 876 Leu Ala Asp Arg Tyr Asp Ala Arg Val Leu Val Asp Glu Ile His Ala 180 185 190 ccg ctg gtg ttc gac ggc acc cat gtg tgc gcc gcc gcc gtc agc gag 924 Pro Leu Val Phe Asp Gly Thr His Val Cys Ala Ala Ala Val Ser Glu 195 200 205 acc gcc gcg cgg gtg tgc gtc acc gtc acg gcg acg tcg aag gcg tgg 972 Thr Ala Ala Arg Val Cys Val Thr Val Thr Ala Thr Ser Lys Ala Trp 210 215 220 aac atc gcg ggc ctc aag tgc gcg cag atg atc ttc tcc aac ccg gcg 1020 Asn Ile Ala Gly Leu Lys Cys Ala Gln Met Ile Phe Ser Asn Pro Ala 225 230 235 240 gac atg gac acg tgg cgg tcg atg acc ggc gtg gcc aag gac ggc acc 1068 Asp Met Asp Thr Trp Arg Ser Met Thr Gly Val Ala Lys Asp Gly Thr 245 250 255 tcc acc ctg ggc atc tgg gcg gcg atc gcc tgc tac cgg gag ggc ggc 1116 Ser Thr Leu Gly Ile Trp Ala Ala Ile Ala Cys Tyr Arg Glu Gly Gly 260 265 270 gac tgc ctc gac gag cag gtc gcg tac ctg cgg gag aac cgc gac tgg 1164 Asp Cys Leu Asp Glu Gln Val Ala Tyr Leu Arg Glu Asn Arg Asp Trp 275 280 285 ctg gcc gag gag ctg ccg cgc cgc gtg ccg ggg ctg cag gtg atc agc 1212 Leu Ala Glu Glu Leu Pro Arg Arg Val Pro Gly Leu Gln Val Ile Ser 290 295 300 ccg gcg gcg acc tat ctc atg tgg atg gac ttc tcc ggc acc tcc atc 1260 Pro Ala Ala Thr Tyr Leu Met Trp Met Asp Phe Ser Gly Thr Ser Ile 305 310 315 320 ggg cac ctg gag cgc ccg gcg gtg tgg ctg cgg gag aac gcg aag gtc 1308 Gly His Leu Glu Arg Pro Ala Val Trp Leu Arg Glu Asn Ala Lys Val 325 330 335 gcc ctc aac gac ggc gtc gcc ttc ggc ccg gga ggc agc cac cac gcg 1356 Ala Leu Asn Asp Gly Val Ala Phe Gly Pro Gly Gly Ser His His Ala 340 345 350 cgg ctg aac ttc gcc acg tcc cgg gag atc ctg gag gag gcc tgc gac 1404 Arg Leu Asn Phe Ala Thr Ser Arg Glu Ile Leu Glu Glu Ala Cys Asp 355 360 365 cgg ctg ggg tct gca ttc gcc cgg tga tttccctacc ctggatttca 1451 Arg Leu Gly Ser Ala Phe Ala Arg 370 375 tgactgacga gcgatacgac gacccgtccg ccacgaccgg cccccagggt cccggactgt 1511 ccatgggtcc gctgggggtc ggcgccgaac cggagaagtc cccggtccgc ccccgtaccg 1571 acgccgaggg ctacctggtc gaaccggagt ccgagcagtt cgagaccccg accccggcgc 1631 ccggtgacgc cccctgggag cgccccgagc cggagtggta caaggaggcc gtgttctacg 1691 aggtcctggt gcgcgccttc tacgacccgg agggcaccgg 1731 <210> 26 <211> 376 <212> PRT <213> Corynebacterium humireducens <400> 26 Met Gln Phe Pro Thr Leu Asp Gln Leu Lys Asp Arg Arg Thr Arg Lys 1 5 10 15 Trp Thr Val Tyr Ala Asp Asp Val Leu Pro Leu Trp Ile Ala Glu Ser 20 25 30 Asp Phe Phe Thr Cys Pro Pro Val Lys Gln Ala Ile Ala Asp Ala Ile 35 40 45 Asp Arg Glu Ser Phe Gly Tyr Thr Pro Ala Thr Ser Asp Leu Pro Gln 50 55 60 Ala Val Ser Asp Phe Tyr Ala Ala Arg Tyr Gly Trp Arg Pro Asp Pro 65 70 75 80 Ala Leu Ile Val Ala Val Pro Asp Val Val Arg Gly Leu Ala Leu Ala 85 90 95 Ile Glu Tyr Met Thr Arg Pro Asp Ser Gly Val Ile Val Pro Val Pro 100 105 110 Ala Tyr Pro Pro Phe Leu Glu Leu Pro Glu Ala Thr Arg Arg Pro Met 115 120 125 His Gln Ile Asp Ala Tyr Gly Gly Ile Asp Leu Gly Asp Val Glu Lys 130 135 140 Gln Phe Ala Ala Gly Ala Gly Ser Ile Leu Leu Cys Ala Pro Asn Asn 145 150 155 160 Pro Leu Gly Tyr Thr Phe Asp Ala Glu Phe Leu Arg Glu Leu Cys Asp 165 170 175 Leu Ala Asp Arg Tyr Asp Ala Arg Val Leu Val Asp Glu Ile His Ala 180 185 190 Pro Leu Val Phe Asp Gly Thr His Val Cys Ala Ala Ala Val Ser Glu 195 200 205 Thr Ala Ala Arg Val Cys Val Thr Val Thr Ala Thr Ser Lys Ala Trp 210 215 220 Asn Ile Ala Gly Leu Lys Cys Ala Gln Met Ile Phe Ser Asn Pro Ala 225 230 235 240 Asp Met Asp Thr Trp Arg Ser Met Thr Gly Val Ala Lys Asp Gly Thr 245 250 255 Ser Thr Leu Gly Ile Trp Ala Ala Ile Ala Cys Tyr Arg Glu Gly Gly 260 265 270 Asp Cys Leu Asp Glu Gln Val Ala Tyr Leu Arg Glu Asn Arg Asp Trp 275 280 285 Leu Ala Glu Glu Leu Pro Arg Arg Val Pro Gly Leu Gln Val Ile Ser 290 295 300 Pro Ala Ala Thr Tyr Leu Met Trp Met Asp Phe Ser Gly Thr Ser Ile 305 310 315 320 Gly His Leu Glu Arg Pro Ala Val Trp Leu Arg Glu Asn Ala Lys Val 325 330 335 Ala Leu Asn Asp Gly Val Ala Phe Gly Pro Gly Gly Ser His His Ala 340 345 350 Arg Leu Asn Phe Ala Thr Ser Arg Glu Ile Leu Glu Glu Ala Cys Asp 355 360 365 Arg Leu Gly Ser Ala Phe Ala Arg 370 375 <210> 27 <211> 1632 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1332) <223> gene asd <400> 27 cggctggtcc aacgtgctct acgacgacca ggtgggcaag gtctccctcg tgggtgccgg 60 catgaagtcc cacccgggcg tcaccgccga cttcgccgag gcgctccgcg acgccggcgt 120 gaacatcgag ctaatctcca cctcggagat ccgcatctcg gtgctcaccc gcgaggccga 180 cctggacgcc gccgcccgtg ccctgcacga gaagttcgag ctcggcggcg acgccgaggc 240 caccgtctac gccggcaccg gccgctagac aatcccttcc acagaggagc tttcactctc 300 atg acc acc att gct gtc gtc ggt gcc acc ggc cag gtc ggc cgc gtg 348 Met Thr Thr Ile Ala Val Val Gly Ala Thr Gly Gln Val Gly Arg Val 1 5 10 15 atg cgc tcg atc ctg gag gag cgc aac ttc ccg gcc gac aag gtc cgt 396 Met Arg Ser Ile Leu Glu Glu Arg Asn Phe Pro Ala Asp Lys Val Arg 20 25 30 ttc ttc gcg tcc ccg cgc tcc gcg ggc acc gtc atc gag ttc cgc ggt 444 Phe Phe Ala Ser Pro Arg Ser Ala Gly Thr Val Ile Glu Phe Arg Gly 35 40 45 gag gac atc acc gtc gag gac ctc acc cag atc acc ccg gag tcc gtc 492 Glu Asp Ile Thr Val Glu Asp Leu Thr Gln Ile Thr Pro Glu Ser Val 50 55 60 aag gac gtc gac atc gcc ctc ttc tcc gcc ggt ggt tcc acc tcc cgt 540 Lys Asp Val Asp Ile Ala Leu Phe Ser Ala Gly Gly Ser Thr Ser Arg 65 70 75 80 gag tgg gcc ccg gtc ttc gcc gag gcc ggc gcg acc gtc atc gac aac 588 Glu Trp Ala Pro Val Phe Ala Glu Ala Gly Ala Thr Val Ile Asp Asn 85 90 95 tcc tcc gcc tgg cgc aag gac gac gag gtc ccg ctc gtc gtc gcc gag 636 Ser Ser Ala Trp Arg Lys Asp Asp Glu Val Pro Leu Val Val Ala Glu 100 105 110 gtc aac ggc gag gag gcg aag aac ccg tcc aag ggc atc gtc gcc aac 684 Val Asn Gly Glu Glu Ala Lys Asn Pro Ser Lys Gly Ile Val Ala Asn 115 120 125 ccg aac tgc acc acc atg gcc gcc atg ccg gtg ctc aag ccg ctg cac 732 Pro Asn Cys Thr Thr Met Ala Ala Met Pro Val Leu Lys Pro Leu His 130 135 140 gac gcc gcc ggc ctc atc cgc ctg cat gtc tcc tcc tac cag gcc gtc 780 Asp Ala Ala Gly Leu Ile Arg Leu His Val Ser Ser Tyr Gln Ala Val 145 150 155 160 tcc ggc tcc ggc ctc gcc ggt gtg gag acc ctg gcg aag cag acc gcc 828 Ser Gly Ser Gly Leu Ala Gly Val Glu Thr Leu Ala Lys Gln Thr Ala 165 170 175 gag ctc ggt gag gac aac gtc aag ctg gtc cat gac ggt tcc gtc gag 876 Glu Leu Gly Glu Asp Asn Val Lys Leu Val His Asp Gly Ser Val Glu 180 185 190 gcc ccc gcc gac ctg ggt ccc tac gtc gcc ccc atc gcg ttc aac gcc 924 Ala Pro Ala Asp Leu Gly Pro Tyr Val Ala Pro Ile Ala Phe Asn Ala 195 200 205 ctg ccg ctg gcc ggc gcg ctg gtc gat gac ggc tcc ctg gag acg gat 972 Leu Pro Leu Ala Gly Ala Leu Val Asp Asp Gly Ser Leu Glu Thr Asp 210 215 220 gag gag cag aag ctg cgc aac gag tcc cgc aag atc ctg gac atc ccg 1020 Glu Glu Gln Lys Leu Arg Asn Glu Ser Arg Lys Ile Leu Asp Ile Pro 225 230 235 240 ggt ctg cgc gtc gcc ggc acc tgc gtc cgc gtg ccg gtg ttc acc ggt 1068 Gly Leu Arg Val Ala Gly Thr Cys Val Arg Val Pro Val Phe Thr Gly 245 250 255 cac acc ctg acc atc cac gcc gag ttc gag cgt ccg atc acc gtc gac 1116 His Thr Leu Thr Ile His Ala Glu Phe Glu Arg Pro Ile Thr Val Asp 260 265 270 gag gcc aag gcc ctg ctc gcc gac gcc ccg ggt gtc gag ctt gtc gac 1164 Glu Ala Lys Ala Leu Leu Ala Asp Ala Pro Gly Val Glu Leu Val Asp 275 280 285 gtc ccg acc ccg ctg gag gcc gcc ggc aag gac acc tcc ctg gtc ggc 1212 Val Pro Thr Pro Leu Glu Ala Ala Gly Lys Asp Thr Ser Leu Val Gly 290 295 300 cgt atc cgc cag gac cag tcc gtc gac gac aac aag ggc ctc gtg ctc 1260 Arg Ile Arg Gln Asp Gln Ser Val Asp Asp Asn Lys Gly Leu Val Leu 305 310 315 320 gtc gtc tcc ggt gac aac ctg cgc aag ggc gcg gcg ctg aac acg gtg 1308 Val Val Ser Gly Asp Asn Leu Arg Lys Gly Ala Ala Leu Asn Thr Val 325 330 335 cag atc gcg gag ctg ctg gtg tag tccaccgcct ccactgatat cgggcccctc 1362 Gln Ile Ala Glu Leu Leu Val 340 accatgttgt ggtgaggggc ccgttcttgt cggtggtctg gtaggggagg ccttcattct 1422 gtccagacaa ttcttgtggt gtcctggggc gtcggcatgc ggcgtcgggc gggtatcggc 1482 gttcccgttc ctcgtttggc ggttcactgc cggacaatgg aggattttct caggaacagg 1542 gatccctgct gatctcgtat ttcgccccgg aactccgctg gacgctcagc ttgtccccag 1602 aattggggtc tttccgcagc gaattgtgag 1632 <210> 28 <211> 343 <212> PRT <213> Corynebacterium humireducens <400> 28 Met Thr Thr Ile Ala Val Val Gly Ala Thr Gly Gln Val Gly Arg Val 1 5 10 15 Met Arg Ser Ile Leu Glu Glu Arg Asn Phe Pro Ala Asp Lys Val Arg 20 25 30 Phe Phe Ala Ser Pro Arg Ser Ala Gly Thr Val Ile Glu Phe Arg Gly 35 40 45 Glu Asp Ile Thr Val Glu Asp Leu Thr Gln Ile Thr Pro Glu Ser Val 50 55 60 Lys Asp Val Asp Ile Ala Leu Phe Ser Ala Gly Gly Ser Thr Ser Arg 65 70 75 80 Glu Trp Ala Pro Val Phe Ala Glu Ala Gly Ala Thr Val Ile Asp Asn 85 90 95 Ser Ser Ala Trp Arg Lys Asp Asp Glu Val Pro Leu Val Val Ala Glu 100 105 110 Val Asn Gly Glu Glu Ala Lys Asn Pro Ser Lys Gly Ile Val Ala Asn 115 120 125 Pro Asn Cys Thr Thr Met Ala Ala Met Pro Val Leu Lys Pro Leu His 130 135 140 Asp Ala Ala Gly Leu Ile Arg Leu His Val Ser Ser Tyr Gln Ala Val 145 150 155 160 Ser Gly Ser Gly Leu Ala Gly Val Glu Thr Leu Ala Lys Gln Thr Ala 165 170 175 Glu Leu Gly Glu Asp Asn Val Lys Leu Val His Asp Gly Ser Val Glu 180 185 190 Ala Pro Ala Asp Leu Gly Pro Tyr Val Ala Pro Ile Ala Phe Asn Ala 195 200 205 Leu Pro Leu Ala Gly Ala Leu Val Asp Asp Gly Ser Leu Glu Thr Asp 210 215 220 Glu Glu Gln Lys Leu Arg Asn Glu Ser Arg Lys Ile Leu Asp Ile Pro 225 230 235 240 Gly Leu Arg Val Ala Gly Thr Cys Val Arg Val Pro Val Phe Thr Gly 245 250 255 His Thr Leu Thr Ile His Ala Glu Phe Glu Arg Pro Ile Thr Val Asp 260 265 270 Glu Ala Lys Ala Leu Leu Ala Asp Ala Pro Gly Val Glu Leu Val Asp 275 280 285 Val Pro Thr Pro Leu Glu Ala Ala Gly Lys Asp Thr Ser Leu Val Gly 290 295 300 Arg Ile Arg Gln Asp Gln Ser Val Asp Asp Asn Lys Gly Leu Val Leu 305 310 315 320 Val Val Ser Gly Asp Asn Leu Arg Lys Gly Ala Ala Leu Asn Thr Val 325 330 335 Gln Ile Ala Glu Leu Leu Val 340 <210> 29 <211> 1725 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1425) <223> gene brnE <400> 29 gatctcgttg gccttgcccg tggcaccgac ggcggtgagg gcgtcgagga cctcgcggga 60 ggcggcgtcg tcgagaagga cggaggccgg cagctccggg ccgtcctcac cgcagaggac 120 ggggacgagc agtgcgtcgg gggtcttcgg ggccttcttg gccagctcga cggcgggggt 180 ggtgccgcgg gcgggaaggg tgaaagtggg gttcgccatg gaaagtacct cctgagattg 240 acaagcgatg aacccagctt atagagatca cctctgggtc gtcggtgtag cgtgttcccc 300 atg acg tct ctc aat ttc acc gtg aac cgt acg agc acc ccg acg tcc 348 Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser 1 5 10 15 gat gag gcg cgc gag gag att ctg cag aat ccg cgg ttc ggc aag aac 396 Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn 20 25 30 ttc acc gat cac atg gtc acc atc gag tgg tcc gag gac aag ggc tgg 444 Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp 35 40 45 cac gac gcc cgc gtg cgt ccc tac gag gcc atc ccg atg gat ccc gcg 492 His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala 50 55 60 acc acg gtg ttc cac tac ggc cag gcg atc ttc gag ggc atc aag gcc 540 Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala 65 70 75 80 tac cgt cag ccg gac ggc tcc atc gcc acc ttc cgc ccg gag cgc aac 588 Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn 85 90 95 gcc gag cgc atg cag cgt tcc gcc gag cgc atg gcc atg ccg ccg ctg 636 Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu 100 105 110 ccg acc gag gac ttc ctc gag acg ctc cgc ctg ctt gtc gac gtc gac 684 Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp 115 120 125 cgt gac tgg gtc ccc gcc gcc ggc ggc gag gcc agc ctc tac ctg cgt 732 Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg 130 135 140 ccc ttc atg atc tcc acc gag gtc tcc ctg ggc gtc agc ccg gcc aac 780 Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn 145 150 155 160 cgg tac acc ttc ttc gtc atc gcc tcc ccg gtc ggg gcg tac ttc acc 828 Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr 165 170 175 ggt ggc gtc aag ccg gtc tcc gtg tgg ctg agc gag gac tac gtc cgt 876 Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg 180 185 190 gcc gcc ccg ggt ggc acc ggt gcc gcg aag ttc gcc ggc aac tac gcc 924 Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala 195 200 205 gcc tcc ctg ctc gcg cag gcg cag gcg gag gag aag ggc tgc gac cag 972 Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln 210 215 220 gtc gtg tgg ctc gac gcc atc gag cac acc tac atc gag gag atg ggt 1020 Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly 225 230 235 240 ggc atg aac ctg ttc ttc gtg tac ggc tcg ggt gac gac gtc acc gtg 1068 Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val 245 250 255 gcc acc ccc gag ctc tcc ggc tcc ctc ctg ccg ggc gtc acc cgc gac 1116 Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp 260 265 270 tcc ctc ctg cag gtc gcc cgc gat ctc ggc cac cgc acc gag gag cgg 1164 Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg 275 280 285 agg atc tcc aag ttc gac tgg cgc gac gac gtc gcc tcc ggt gcg atg 1212 Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met 290 295 300 acc gag gcc atg gcc tgc ggc aca gcc gcc gtg atc acc ccg gtc ggc 1260 Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly 305 310 315 320 cgg gtc ctg tcc aac gac ggc gag ttc cag atc aat gac aac cag ccg 1308 Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro 325 330 335 ggt gag gtc acc atg gcc atg cgc gag cgt ctc acc gac atc cag cgc 1356 Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg 340 345 350 ggc ctg tac gag gac acc cac ggc tgg gtg cac acc ctc gtc ccg gct 1404 Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala 355 360 365 gag gac cag ccg gcg gac tag tcggccagca gctcggcggc ggcccgcacc 1455 Glu Asp Gln Pro Ala Asp 370 agcgggagcg ccgctagcgc gccggtgccc tggccggcgt tcatgtccag ggcgacgaga 1515 ggcgtgagtt cgagggcctg cagcgcgatg aggtgcgcgg gctccggggt cagctgccct 1575 gcctggcacc agggggcggt gccgggggag agacgttccg cgaggtaggc ggcgacggtg 1635 acgggggcgc cgtcgagaag cacgggggtg cggcgtgccg cggcctgcgc gatgaaggcc 1695 accagtgcca ccaggtccgg ggaggcgatc 1725 <210> 30 <211> 374 <212> PRT <213> Corynebacterium humireducens <400> 30 Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser 1 5 10 15 Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn 20 25 30 Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp 35 40 45 His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala 50 55 60 Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala 65 70 75 80 Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn 85 90 95 Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu 100 105 110 Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp 115 120 125 Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg 130 135 140 Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn 145 150 155 160 Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr 165 170 175 Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg 180 185 190 Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala 195 200 205 Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln 210 215 220 Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly 225 230 235 240 Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val 245 250 255 Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp 260 265 270 Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg 275 280 285 Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met 290 295 300 Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly 305 310 315 320 Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro 325 330 335 Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg 340 345 350 Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala 355 360 365 Glu Asp Gln Pro Ala Asp 370 <210> 31 <211> 1296 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(996) <223> gen brnF <400> 31 cgacatggag acggccccgc agctcaaccg cctggccaac cgcctcgccc acgtcgacct 60 gcgcgccgat cccggtgaca tcgccgagct cgtcgagggc atgcgtctgt tcgccggtta 120 cgccgagtgg gcgcccggcc agctcgacgg ggagatcgac cgcggcgact ggttcgtcac 180 cccggcgctg cccaccgacg tcatcgtccc gggccgcacc gacctgtggg gcgacgtcat 240 gcgccgccag cagccgccgc tgccgctctt ctcgacgttc cccgccgacc tcatggacaa 300 ttg acc ccc gcg ttc cct gac gtc cgt gcc gga ctg cgg gac gcc tgg 348 Leu Thr Pro Ala Phe Pro Asp Val Arg Ala Gly Leu Arg Asp Ala Trp 1 5 10 15 gcc gtc gcg ctc ggg ctg gtc ccc ctg ggg ctg gcc ttc ggc ctg ctg 396 Ala Val Ala Leu Gly Leu Val Pro Leu Gly Leu Ala Phe Gly Leu Leu 20 25 30 atg acg cag gtg ggt ttc gcc tgg tgg tgg acg ccg gtc ttc tcg gtg 444 Met Thr Gln Val Gly Phe Ala Trp Trp Trp Thr Pro Val Phe Ser Val 35 40 45 ctc atc tac gcc ggg tcc atg gag ttc ctg gcg gtc aac ctg gtg ctc 492 Leu Ile Tyr Ala Gly Ser Met Glu Phe Leu Ala Val Asn Leu Val Leu 50 55 60 gcc ggc gtc ggc ccc ctg tcg acg gcg ctg acc gcc ttc atg gtc aac 540 Ala Gly Val Gly Pro Leu Ser Thr Ala Leu Thr Ala Phe Met Val Asn 65 70 75 80 ttc cgg cac atc ttc tac ggc ctc acc ttc ccc cgg cac ctc gtc cgg 588 Phe Arg His Ile Phe Tyr Gly Leu Thr Phe Pro Arg His Leu Val Arg 85 90 95 ggc cgg gcc ggc cgt gct tac tcc acg tac tcg ctg acc gac gag tcc 636 Gly Arg Ala Gly Arg Ala Tyr Ser Thr Tyr Ser Leu Thr Asp Glu Ser 100 105 110 tac gcc atc gcc tcc gcc cac cct ccg cgc tcc ggt acg cag gtg ctg 684 Tyr Ala Ile Ala Ser Ala His Pro Pro Arg Ser Gly Thr Gln Val Leu 115 120 125 acg atc cag ctc ttc tgc cag gcg atg tgg gtg gtg ccc ggc atc atc 732 Thr Ile Gln Leu Phe Cys Gln Ala Met Trp Val Val Pro Gly Ile Ile 130 135 140 ggg gcg ctc gtc ggt gag gtc atc ccg gcg ggc atc cag ggc atg gag 780 Gly Ala Leu Val Gly Glu Val Ile Pro Ala Gly Ile Gln Gly Met Glu 145 150 155 160 ttc gca ctc gtc gca ctg ttc gtg gtg ctg gcg tgg gag acc ttc cgg 828 Phe Ala Leu Val Ala Leu Phe Val Val Leu Ala Trp Glu Thr Phe Arg 165 170 175 ggc agc cgg gac ctc tcc ctg ccg ctg ctc gcc ggt ggc ctg gca ctc 876 Gly Ser Arg Asp Leu Ser Leu Pro Leu Leu Ala Gly Gly Leu Ala Leu 180 185 190 gtt gcc ggc ctg gtg ctc ccg ggg cag atg ctc atg gtg gcg ctg agc 924 Val Ala Gly Leu Val Leu Pro Gly Gln Met Leu Met Val Ala Leu Ser 195 200 205 gcc tac ttc ctc gtc ctc atc ggc cgg cac ctc tcc ccc gcc ctg gac 972 Ala Tyr Phe Leu Val Leu Ile Gly Arg His Leu Ser Pro Ala Leu Asp 210 215 220 cgt cgc ctg gag tgg agg gtc tga tgtacgggct tcccgagggg gtcaccctca 1026 Arg Arg Leu Glu Trp Arg Val 225 230 cccagacgtt ggcggtcctg ctgcccatcg gggtggtcac ggtggcgctg cgctggctgc 1086 ccttcgcctt tgtcagggcg ctgcgcgaca accagttctt cggtctgctc gcccggatga 1146 tgccggtggg agtgatgacc gtgctggtgg tgtacacgct cctcgggcag cgttcggcgc 1206 ccggtggcct ggcggcggcc ctcatcgggg tcgcggtgac cctggggctg cacgcctggc 1266 ggcgggactc tgggctctcg atcctcggag 1296 <210> 32 <211> 231 <212> PRT <213> Corynebacterium humireducens <400> 32 Leu Thr Pro Ala Phe Pro Asp Val Arg Ala Gly Leu Arg Asp Ala Trp 1 5 10 15 Ala Val Ala Leu Gly Leu Val Pro Leu Gly Leu Ala Phe Gly Leu Leu 20 25 30 Met Thr Gln Val Gly Phe Ala Trp Trp Trp Thr Pro Val Phe Ser Val 35 40 45 Leu Ile Tyr Ala Gly Ser Met Glu Phe Leu Ala Val Asn Leu Val Leu 50 55 60 Ala Gly Val Gly Pro Leu Ser Thr Ala Leu Thr Ala Phe Met Val Asn 65 70 75 80 Phe Arg His Ile Phe Tyr Gly Leu Thr Phe Pro Arg His Leu Val Arg 85 90 95 Gly Arg Ala Gly Arg Ala Tyr Ser Thr Tyr Ser Leu Thr Asp Glu Ser 100 105 110 Tyr Ala Ile Ala Ser Ala His Pro Pro Arg Ser Gly Thr Gln Val Leu 115 120 125 Thr Ile Gln Leu Phe Cys Gln Ala Met Trp Val Val Pro Gly Ile Ile 130 135 140 Gly Ala Leu Val Gly Glu Val Ile Pro Ala Gly Ile Gln Gly Met Glu 145 150 155 160 Phe Ala Leu Val Ala Leu Phe Val Val Leu Ala Trp Glu Thr Phe Arg 165 170 175 Gly Ser Arg Asp Leu Ser Leu Pro Leu Leu Ala Gly Gly Leu Ala Leu 180 185 190 Val Ala Gly Leu Val Leu Pro Gly Gln Met Leu Met Val Ala Leu Ser 195 200 205 Ala Tyr Phe Leu Val Leu Ile Gly Arg His Leu Ser Pro Ala Leu Asp 210 215 220 Arg Arg Leu Glu Trp Arg Val 225 230 <210> 33 <211> 1200 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(900) <223> gene cysE <400> 33 tggaccgcaa ggtcctcgac gacgtcatca ccgtcaccaa cgaggacgcc atcgagacct 60 cccgtcagct ggcggtcaag gacggcatcc tgggcggtat ctcctccggc ggcaacgtct 120 gggccgcgct ggagctggcg aagaaggagg agaacgcggg caagaccatc gtcaccgtcg 180 tcaccgacta cggcgagcgt tacgtctcca ccgtcctgta cgaggacatt cgcgactagg 240 aaacggctta ggcccccgca gcgcccacca ggcgacgggc aagcaagtag actctgggcc 300 atg ctg ctg ttg aac atc gcg aag atg gtc cgg gag gac ctg cgc aac 348 Met Leu Leu Leu Asn Ile Ala Lys Met Val Arg Glu Asp Leu Arg Asn 1 5 10 15 gcc cgc gac cac gat ccc gcc gcc cgc ggc gac atc gag aac gcc gtc 396 Ala Arg Asp His Asp Pro Ala Ala Arg Gly Asp Ile Glu Asn Ala Val 20 25 30 gtc tac tcg ggc ctg cac gcc atc tgg gca cac cgt gtc tcc cac tgg 444 Val Tyr Ser Gly Leu His Ala Ile Trp Ala His Arg Val Ser His Trp 35 40 45 atg tgg gag cgc ggt ctc cgc ggc ccc gcg cga atc ctc gcg cag ctc 492 Met Trp Glu Arg Gly Leu Arg Gly Pro Ala Arg Ile Leu Ala Gln Leu 50 55 60 aac cgg ttc ttc acg ggc atc gag atc cac ccc ggt gcc acc atc ggc 540 Asn Arg Phe Phe Thr Gly Ile Glu Ile His Pro Gly Ala Thr Ile Gly 65 70 75 80 cgc cgt ttc ttc atc gac cac ggc atg ggc atc gtc atc ggc gag acc 588 Arg Arg Phe Phe Ile Asp His Gly Met Gly Ile Val Ile Gly Glu Thr 85 90 95 acc gag atc ggc gac ggc gtc atg ctc tac cac ggc gtc acc ctg ggt 636 Thr Glu Ile Gly Asp Gly Val Met Leu Tyr His Gly Val Thr Leu Gly 100 105 110 ggt cag gtg ctc acg cag acc aag cgc cac ccg acc atc gag gac aac 684 Gly Gln Val Leu Thr Gln Thr Lys Arg His Pro Thr Ile Glu Asp Asn 115 120 125 gtc acc atc ggt gcc ggc gcc aag gtg ctc ggc ccg atc acc atc ggc 732 Val Thr Ile Gly Ala Gly Ala Lys Val Leu Gly Pro Ile Thr Ile Gly 130 135 140 aag ggc tcc gcg atc ggc gcc aac gcc gtg gtg acc aag gac gtc ccg 780 Lys Gly Ser Ala Ile Gly Ala Asn Ala Val Val Thr Lys Asp Val Pro 145 150 155 160 gcg gac cac atc gcc acg ggc atc ccg gcg aag aac cgc ccg cgc cgc 828 Ala Asp His Ile Ala Thr Gly Ile Pro Ala Lys Asn Arg Pro Arg Arg 165 170 175 cag tcc gag cgc atc aag ctc gtc gat ccg gac tac tac acc tcc aac 876 Gln Ser Glu Arg Ile Lys Leu Val Asp Pro Asp Tyr Tyr Thr Ser Asn 180 185 190 gat ccg gtc gac tac agc atc tag acacaaggaa ggccccggtt tcccggggcc 930 Asp Pro Val Asp Tyr Ser Ile 195 tttttgtgct tcacgacgtc ggcgcgtgcc cgacgtcacc cgacgaggtc gcggtactcc 990 tcgttcttgc tgatgaagtg ctcgaccgcg gaacagctcg ggcgcacccg cgccccctcc 1050 tcccgggccc agtcgagggc ctcgcggatg agcggggtgg acagcgcacg tccgcggtag 1110 gcctggtcca ccacggtgtg gtggaagtcc agtaggccac cgtggcggac gtaggaggcg 1170 tagccgacct cccggtcgtc cacggagatg 1200 <210> 34 <211> 199 <212> PRT <213> Corynebacterium humireducens <400> 34 Met Leu Leu Leu Asn Ile Ala Lys Met Val Arg Glu Asp Leu Arg Asn 1 5 10 15 Ala Arg Asp His Asp Pro Ala Ala Arg Gly Asp Ile Glu Asn Ala Val 20 25 30 Val Tyr Ser Gly Leu His Ala Ile Trp Ala His Arg Val Ser His Trp 35 40 45 Met Trp Glu Arg Gly Leu Arg Gly Pro Ala Arg Ile Leu Ala Gln Leu 50 55 60 Asn Arg Phe Phe Thr Gly Ile Glu Ile His Pro Gly Ala Thr Ile Gly 65 70 75 80 Arg Arg Phe Phe Ile Asp His Gly Met Gly Ile Val Ile Gly Glu Thr 85 90 95 Thr Glu Ile Gly Asp Gly Val Met Leu Tyr His Gly Val Thr Leu Gly 100 105 110 Gly Gln Val Leu Thr Gln Thr Lys Arg His Pro Thr Ile Glu Asp Asn 115 120 125 Val Thr Ile Gly Ala Gly Ala Lys Val Leu Gly Pro Ile Thr Ile Gly 130 135 140 Lys Gly Ser Ala Ile Gly Ala Asn Ala Val Val Thr Lys Asp Val Pro 145 150 155 160 Ala Asp His Ile Ala Thr Gly Ile Pro Ala Lys Asn Arg Pro Arg Arg 165 170 175 Gln Ser Glu Arg Ile Lys Leu Val Asp Pro Asp Tyr Tyr Thr Ser Asn 180 185 190 Asp Pro Val Asp Tyr Ser Ile 195 <210> 35 <211> 1536 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1236) <223> gene cysK <400> 35 cctccgggta cacccgtaca ggtgcacccg atgggcacat gaacgggggt aactttcctt 60 tacagatggg cattataacg cgacttcggg ggtaaacgga atcgaatccg ccgtaacttg 120 cgcattcaag accgttctga tcggaacaac ggcacccact gaaggggtga tatcccgcgc 180 cggagaccac ccccgcaacc ttggagaaac catgtggtct gccagggaca taccatcctg 240 tctactcaca tggtgtacgc tgacaccacc aacgtcagac gaagcaagga agcagtcacc 300 atg agc aag atc tac gac aac atc ctc gac acc atc gga aac acc ccg 348 Met Ser Lys Ile Tyr Asp Asn Ile Leu Asp Thr Ile Gly Asn Thr Pro 1 5 10 15 ctg gtg cgc ctc aac ggc ctc acc gag ggc ctc cag gcg gag gtg ctc 396 Leu Val Arg Leu Asn Gly Leu Thr Glu Gly Leu Gln Ala Glu Val Leu 20 25 30 gtg aag gtc gag tcc ttc aac ccg gcg aac tcc gtc aag gac cgc atc 444 Val Lys Val Glu Ser Phe Asn Pro Ala Asn Ser Val Lys Asp Arg Ile 35 40 45 ggc aag gcg atc atc gac gcc gcc gag gcc gac ggg tcc ctc cag ccg 492 Gly Lys Ala Ile Ile Asp Ala Ala Glu Ala Asp Gly Ser Leu Gln Pro 50 55 60 ggc ggc acc atc gtg gag gcc acc tcc ggc aac acc ggc atc gcc ctc 540 Gly Gly Thr Ile Val Glu Ala Thr Ser Gly Asn Thr Gly Ile Ala Leu 65 70 75 80 gcc ctc gtg ggc gcg gcc cgc ggc tac aac gtg atc ctc acc atg ccg 588 Ala Leu Val Gly Ala Ala Arg Gly Tyr Asn Val Ile Leu Thr Met Pro 85 90 95 gag acc atg tcc aac gag cgt cgc gtc atg ctc cgc gcc tac ggc gct 636 Glu Thr Met Ser Asn Glu Arg Arg Val Met Leu Arg Ala Tyr Gly Ala 100 105 110 cag atc gtc ctg acc ccg ggc gcg gcc ggc atg cag ggc gcc gtc gac 684 Gln Ile Val Leu Thr Pro Gly Ala Ala Gly Met Gln Gly Ala Val Asp 115 120 125 aag gcg aac gag atc gtc gcc gcc gag gac aac gcc atc ctc gcg aac 732 Lys Ala Asn Glu Ile Val Ala Ala Glu Asp Asn Ala Ile Leu Ala Asn 130 135 140 cag ttc gcg cac ccg gcg aac ctg cgg atc cac cgc gag acc acc gcc 780 Gln Phe Ala His Pro Ala Asn Leu Arg Ile His Arg Glu Thr Thr Ala 145 150 155 160 gag gag atc tgg aac gac acc gac ggc gag gtc gac atc ttc gtc gcg 828 Glu Glu Ile Trp Asn Asp Thr Asp Gly Glu Val Asp Ile Phe Val Ala 165 170 175 ggc atc ggc acg ggc ggc acc gtc tcc ggc gtc ggc cag gtg ctc aag 876 Gly Ile Gly Thr Gly Gly Thr Val Ser Gly Val Gly Gln Val Leu Lys 180 185 190 gag cgc aag ccg gag gtc cag atc tac gcc gtc gag ccg gcc gcg tcc 924 Glu Arg Lys Pro Glu Val Gln Ile Tyr Ala Val Glu Pro Ala Ala Ser 195 200 205 ccg ctg ctg tcc gcc ggc aag gcg ggc ccg cac aag atc cag ggc atc 972 Pro Leu Leu Ser Ala Gly Lys Ala Gly Pro His Lys Ile Gln Gly Ile 210 215 220 ggc gcc aac ttc atc ccg gag gtc ctg gac cgc aag gtc ctc gac gac 1020 Gly Ala Asn Phe Ile Pro Glu Val Leu Asp Arg Lys Val Leu Asp Asp 225 230 235 240 gtc atc acc gtc acc aac gag gac gcc atc gag acc tcc cgt cag ctg 1068 Val Ile Thr Val Thr Asn Glu Asp Ala Ile Glu Thr Ser Arg Gln Leu 245 250 255 gcg gtc aag gac ggc atc ctg ggc ggt atc tcc tcc ggc ggc aac gtc 1116 Ala Val Lys Asp Gly Ile Leu Gly Gly Ile Ser Ser Gly Gly Asn Val 260 265 270 tgg gcc gcg ctg gag ctg gcg aag aag gag gag aac gcg ggc aag acc 1164 Trp Ala Ala Leu Glu Leu Ala Lys Lys Glu Glu Asn Ala Gly Lys Thr 275 280 285 atc gtc acc gtc gtc acc gac tac ggc gag cgt tac gtc tcc acc gtc 1212 Ile Val Thr Val Val Thr Asp Tyr Gly Glu Arg Tyr Val Ser Thr Val 290 295 300 ctg tac gag gac att cgc gac tag gaaacggctt aggcccccgc agcgcccacc 1266 Leu Tyr Glu Asp Ile Arg Asp 305 310 aggcgacggg caagcaagta gactctgggc catgctgctg ttgaacatcg cgaagatggt 1326 ccgggaggac ctgcgcaacg cccgcgacca cgatcccgcc gcccgcggcg acatcgagaa 1386 cgccgtcgtc tactcgggcc tgcacgccat ctgggcacac cgtgtctccc actggatgtg 1446 ggagcgcggt ctccgcggcc ccgcgcgaat cctcgcgcag ctcaaccggt tcttcacggg 1506 catcgagatc caccccggtg ccaccatcgg 1536 <210> 36 <211> 311 <212> PRT <213> Corynebacterium humireducens <400> 36 Met Ser Lys Ile Tyr Asp Asn Ile Leu Asp Thr Ile Gly Asn Thr Pro 1 5 10 15 Leu Val Arg Leu Asn Gly Leu Thr Glu Gly Leu Gln Ala Glu Val Leu 20 25 30 Val Lys Val Glu Ser Phe Asn Pro Ala Asn Ser Val Lys Asp Arg Ile 35 40 45 Gly Lys Ala Ile Ile Asp Ala Ala Glu Ala Asp Gly Ser Leu Gln Pro 50 55 60 Gly Gly Thr Ile Val Glu Ala Thr Ser Gly Asn Thr Gly Ile Ala Leu 65 70 75 80 Ala Leu Val Gly Ala Ala Arg Gly Tyr Asn Val Ile Leu Thr Met Pro 85 90 95 Glu Thr Met Ser Asn Glu Arg Arg Val Met Leu Arg Ala Tyr Gly Ala 100 105 110 Gln Ile Val Leu Thr Pro Gly Ala Ala Gly Met Gln Gly Ala Val Asp 115 120 125 Lys Ala Asn Glu Ile Val Ala Ala Glu Asp Asn Ala Ile Leu Ala Asn 130 135 140 Gln Phe Ala His Pro Ala Asn Leu Arg Ile His Arg Glu Thr Thr Ala 145 150 155 160 Glu Glu Ile Trp Asn Asp Thr Asp Gly Glu Val Asp Ile Phe Val Ala 165 170 175 Gly Ile Gly Thr Gly Gly Thr Val Ser Gly Val Gly Gln Val Leu Lys 180 185 190 Glu Arg Lys Pro Glu Val Gln Ile Tyr Ala Val Glu Pro Ala Ala Ser 195 200 205 Pro Leu Leu Ser Ala Gly Lys Ala Gly Pro His Lys Ile Gln Gly Ile 210 215 220 Gly Ala Asn Phe Ile Pro Glu Val Leu Asp Arg Lys Val Leu Asp Asp 225 230 235 240 Val Ile Thr Val Thr Asn Glu Asp Ala Ile Glu Thr Ser Arg Gln Leu 245 250 255 Ala Val Lys Asp Gly Ile Leu Gly Gly Ile Ser Ser Gly Gly Asn Val 260 265 270 Trp Ala Ala Leu Glu Leu Ala Lys Lys Glu Glu Asn Ala Gly Lys Thr 275 280 285 Ile Val Thr Val Val Thr Asp Tyr Gly Glu Arg Tyr Val Ser Thr Val 290 295 300 Leu Tyr Glu Asp Ile Arg Asp 305 310 <210> 37 <211> 999 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(699) <223> gene gcvH <400> 37 cagggagccg accagcgcca tcgtcgggct ccggggtctg gggcgccgcg cggcccgcga 60 gggcgccgag gtctacctcg acggcgaaca ggtcggcgtg gtcacctccg ggcagccggc 120 gccggtgctc ggctacccga tcgcactcgc ccgcctgcgc cccgacgtca ccgcggccgg 180 cacggcactg gaggtggaca tccgtgggag gcggcacccc ttcgagatcg tcgacctgcc 240 cttctaccgc cgcgccgcac gataacctga acccaccacc aacggaaagg ctcccccacc 300 atg gcc acc gag cac tcc ctg ccc ggg aac tac tcc tac tcc gag gac 348 Met Ala Thr Glu His Ser Leu Pro Gly Asn Tyr Ser Tyr Ser Glu Asp 1 5 10 15 cac gag tgg atc gac gcg acg ccc gag gac gcc gtc ggc gcg acc gtg 396 His Glu Trp Ile Asp Ala Thr Pro Glu Asp Ala Val Gly Ala Thr Val 20 25 30 aag gtc ggc atc acc tcc gtg gcc acc gaa cgc ctc ggc gag gtc gtc 444 Lys Val Gly Ile Thr Ser Val Ala Thr Glu Arg Leu Gly Glu Val Val 35 40 45 tac gcg gag ctc ccg cag gtc ggt gac gcc gtc acc gcc ggt gaa ccg 492 Tyr Ala Glu Leu Pro Gln Val Gly Asp Ala Val Thr Ala Gly Glu Pro 50 55 60 tgc ggc gag gtc gag tcc acg aag tcg gtc tcc gac ctc tac gcg ccg 540 Cys Gly Glu Val Glu Ser Thr Lys Ser Val Ser Asp Leu Tyr Ala Pro 65 70 75 80 gtc acg ggc acc gtc acc gag gtc aac gac gcg gtc cac gac gac tac 588 Val Thr Gly Thr Val Thr Glu Val Asn Asp Ala Val His Asp Asp Tyr 85 90 95 gcc gtg atc aac gac gac ccc ttc ggc gcg ggc tgg ctc ttc gcc gtc 636 Ala Val Ile Asn Asp Asp Pro Phe Gly Ala Gly Trp Leu Phe Ala Val 100 105 110 gag gtc gac gag gtc gga ccg ctc atg acc gcc gcc gag tac gcg gag 684 Glu Val Asp Glu Val Gly Pro Leu Met Thr Ala Ala Glu Tyr Ala Glu 115 120 125 gcc aac ggc gtg tga ccgcccggga ccggtaccct ggtcccatca tgactgcacc 739 Ala Asn Gly Val 130 ccgtgagccc ttcttccctg cggaccgttc catccgtgcc tccggcgagc ccccggagat 799 ccgttatctc ggcgtcgtcg actaccagga ggcctggaat ctgcaggccg agctggcgtc 859 gcaacgcgcg gccggggaga tcggtgacca gctcctcatc ctcgagcacc cgagcgtgta 919 caccgccggc aagcgcacgc agccggagga ccgccccacc aacggcctgc cggtcatcga 979 cgtcgaccgg ggtggccgca 999 <210> 38 <211> 132 <212> PRT <213> Corynebacterium humireducens <400> 38 Met Ala Thr Glu His Ser Leu Pro Gly Asn Tyr Ser Tyr Ser Glu Asp 1 5 10 15 His Glu Trp Ile Asp Ala Thr Pro Glu Asp Ala Val Gly Ala Thr Val 20 25 30 Lys Val Gly Ile Thr Ser Val Ala Thr Glu Arg Leu Gly Glu Val Val 35 40 45 Tyr Ala Glu Leu Pro Gln Val Gly Asp Ala Val Thr Ala Gly Glu Pro 50 55 60 Cys Gly Glu Val Glu Ser Thr Lys Ser Val Ser Asp Leu Tyr Ala Pro 65 70 75 80 Val Thr Gly Thr Val Thr Glu Val Asn Asp Ala Val His Asp Asp Tyr 85 90 95 Ala Val Ile Asn Asp Asp Pro Phe Gly Ala Gly Trp Leu Phe Ala Val 100 105 110 Glu Val Asp Glu Val Gly Pro Leu Met Thr Ala Ala Glu Tyr Ala Glu 115 120 125 Ala Asn Gly Val 130 <210> 39 <211> 3462 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(3162) <223> gene gcvP <400> 39 ctgggcaccg cggccatcga gaagcgtccg gtcgtggtga ccgatgacgg cgtcgactcg 60 atcgccatcc gccagatgtg ctacctgccg ttcacctacg accaccaggt catcgacggt 120 gccgacgccg gtcgcttcat caccaccatc aaggaccgcc tggagaccgc ggacttccag 180 tccgacctcg gcctctaagc tctcccgctg agagagaccg ccacctgacc ttcccggtcg 240 ggtggcggtt ttcttcttat aatcggaatc atccggtccc ccatcgagga gcgattccgc 300 atg gcc ctg cac ccg atc acc gat tcc gcc tcc tac ctg gcc cga cac 348 Met Ala Leu His Pro Ile Thr Asp Ser Ala Ser Tyr Leu Ala Arg His 1 5 10 15 ctc ggc ccc gac acc gcg gag gag cag gtc atg ctg ggg gcg ctg ggc 396 Leu Gly Pro Asp Thr Ala Glu Glu Gln Val Met Leu Gly Ala Leu Gly 20 25 30 tac ggt tcc ctc gcc gag ctt gtc gac gcc gcc ctg ccc acc gac atc 444 Tyr Gly Ser Leu Ala Glu Leu Val Asp Ala Ala Leu Pro Thr Asp Ile 35 40 45 cgc ctc acc gaa cct ctc ccg cgg cac gcg ccc ctc tcg gag acg gag 492 Arg Leu Thr Glu Pro Leu Pro Arg His Ala Pro Leu Ser Glu Thr Glu 50 55 60 gcg cag gcg cga ctg cgg gag tac gcc tcg cgg aac acg gtg ctc cgt 540 Ala Gln Ala Arg Leu Arg Glu Tyr Ala Ser Arg Asn Thr Val Leu Arg 65 70 75 80 tcc ttt tac ggg cag ggt ttc tcc gac acg gtc act ccc ccg gtg atc 588 Ser Phe Tyr Gly Gln Gly Phe Ser Asp Thr Val Thr Pro Pro Val Ile 85 90 95 cgc cgc ggg gtg ctc gag gcc ccg ggc tgg tac acc gcg tac acg ccg 636 Arg Arg Gly Val Leu Glu Ala Pro Gly Trp Tyr Thr Ala Tyr Thr Pro 100 105 110 tac cag ccg gag atc tcg cag ggc cgc ctc gag gcg ctg ctc aac ttc 684 Tyr Gln Pro Glu Ile Ser Gln Gly Arg Leu Glu Ala Leu Leu Asn Phe 115 120 125 cag acg atg gtc tcc gag ctc acc ggc ctg ccg gtg gcg aac gcc tcg 732 Gln Thr Met Val Ser Glu Leu Thr Gly Leu Pro Val Ala Asn Ala Ser 130 135 140 ctc ctc gac gag gct tcc gcc acc gcc gaa gcc gtc ggc ctc atg tcg 780 Leu Leu Asp Glu Ala Ser Ala Thr Ala Glu Ala Val Gly Leu Met Ser 145 150 155 160 cgg gcg cag agg aag ggg cgg cgg gtg ctt ctc gac gcc cgc ctc cac 828 Arg Ala Gln Arg Lys Gly Arg Arg Val Leu Leu Asp Ala Arg Leu His 165 170 175 ccc cag gtc ctc gcc gtc gct gcg gag cgg gcc cgg gcg atc gac ctg 876 Pro Gln Val Leu Ala Val Ala Ala Glu Arg Ala Arg Ala Ile Asp Leu 180 185 190 gag gtc gcc gtc gag gac ctg tcc ggc ccg ctg gtg ggt gag gat ctc 924 Glu Val Ala Val Glu Asp Leu Ser Gly Pro Leu Val Gly Glu Asp Leu 195 200 205 tgc ggc gtc gtc atc gcc tac ccc ggc acg gag ggc gac atc gcc gat 972 Cys Gly Val Val Ile Ala Tyr Pro Gly Thr Glu Gly Asp Ile Ala Asp 210 215 220 ccc cgc ccg gtc atc gag gcc gtg cac gca cgc ggg ggt ctg gcc acc 1020 Pro Arg Pro Val Ile Glu Ala Val His Ala Arg Gly Gly Leu Ala Thr 225 230 235 240 gtg gtc acc gat ccc ctg tcg ctg ctc ctg ctc gag tcg ccg ggt gag 1068 Val Val Thr Asp Pro Leu Ser Leu Leu Leu Leu Glu Ser Pro Gly Glu 245 250 255 atg ggg gcg gac atc gcg gtg ggc tcc tcg cag cgt ttc ggc gtg ccc 1116 Met Gly Ala Asp Ile Ala Val Gly Ser Ser Gln Arg Phe Gly Val Pro 260 265 270 ctg ttc tac ggt ggc ccg cac gcc gcg tac atg gcc gtc agt gac gcc 1164 Leu Phe Tyr Gly Gly Pro His Ala Ala Tyr Met Ala Val Ser Asp Ala 275 280 285 ctc aag cgc cag atc ccc ggc cgc ctg gtg ggc gtg tcc gtc gac gcc 1212 Leu Lys Arg Gln Ile Pro Gly Arg Leu Val Gly Val Ser Val Asp Ala 290 295 300 gag ggc gcc ccc gcc tac cgg ctc gcg ctg cag acc cgg gag cag cac 1260 Glu Gly Ala Pro Ala Tyr Arg Leu Ala Leu Gln Thr Arg Glu Gln His 305 310 315 320 atc cgt cgc gag cgg gcg acg tcg aac atc tgc acc gcg cag gcg ctg 1308 Ile Arg Arg Glu Arg Ala Thr Ser Asn Ile Cys Thr Ala Gln Ala Leu 325 330 335 ctg gcg gtc acc gcc ggc atg tac gcc gtc tac cac ggt ccc gag ggg 1356 Leu Ala Val Thr Ala Gly Met Tyr Ala Val Tyr His Gly Pro Glu Gly 340 345 350 ttg cgt gcc atc gcg gag cgc gtg cac ggt ctg gcc tgt gac ttc gcg 1404 Leu Arg Ala Ile Ala Glu Arg Val His Gly Leu Ala Cys Asp Phe Ala 355 360 365 gcg gcc gtc cgc gcc gcc ggc ggg acg gtg ctg cac gac gcc ttc ttc 1452 Ala Ala Val Arg Ala Ala Gly Gly Thr Val Leu His Asp Ala Phe Phe 370 375 380 gac acc gtc gcg gtg cgg gtg gag gct gca cga ccg atc gtc ggc acg 1500 Asp Thr Val Ala Val Arg Val Glu Ala Ala Arg Pro Ile Val Gly Thr 385 390 395 400 ctc gcg gag gcg ggc tac ctc gtg cgt gcc gtg gac ggc acg acc gtc 1548 Leu Ala Glu Ala Gly Tyr Leu Val Arg Ala Val Asp Gly Thr Thr Val 405 410 415 ggc gtg agc ttc ggt gag tcg gcg gag gag ggg gac gtc gag aag ctc 1596 Gly Val Ser Phe Gly Glu Ser Ala Glu Glu Gly Asp Val Glu Lys Leu 420 425 430 gcc gcc gtc ttc ggc gcc tcc ctg acg gcg ggc acc cgc tgc atc ccc 1644 Ala Ala Val Phe Gly Ala Ser Leu Thr Ala Gly Thr Arg Cys Ile Pro 435 440 445 gcg tcc gtg gcg cgg cag acg ccg acg ctg acg cac ccg gtc ttc tcc 1692 Ala Ser Val Ala Arg Gln Thr Pro Thr Leu Thr His Pro Val Phe Ser 450 455 460 acg ctc cac tcc gag acg cag atg atg cgc tac ctg cgc acg ctc tcg 1740 Thr Leu His Ser Glu Thr Gln Met Met Arg Tyr Leu Arg Thr Leu Ser 465 470 475 480 gac cgg gat ctc gcg ctc gac cgc acg atg atc ccg ctg ggt tcg tgc 1788 Asp Arg Asp Leu Ala Leu Asp Arg Thr Met Ile Pro Leu Gly Ser Cys 485 490 495 acg atg aag ctc aac ccg acc gcc ggg ctg gag acg atc acg tgg ccg 1836 Thr Met Lys Leu Asn Pro Thr Ala Gly Leu Glu Thr Ile Thr Trp Pro 500 505 510 gag ttc gcc gac atg cac ccc ttc gcc ccc gac gac cag gcc gcg ggc 1884 Glu Phe Ala Asp Met His Pro Phe Ala Pro Asp Asp Gln Ala Ala Gly 515 520 525 tgg ctg gcg ctc atc gcg gag ctg gag ggc tgg ctc gcg gag ctc acc 1932 Trp Leu Ala Leu Ile Ala Glu Leu Glu Gly Trp Leu Ala Glu Leu Thr 530 535 540 gga tac gcg aag gtg tcc gtg cag ccc aac gcg ggt tcc cag ggg gag 1980 Gly Tyr Ala Lys Val Ser Val Gln Pro Asn Ala Gly Ser Gln Gly Glu 545 550 555 560 ctg gcc gga ctg ctg gcc atc cgc cgc tac cac gtg gcc aac ggg gac 2028 Leu Ala Gly Leu Leu Ala Ile Arg Arg Tyr His Val Ala Asn Gly Asp 565 570 575 atg gac cgt gat atc tgc ctg gtc ccg gcc tcg gcg cac ggc acg aac 2076 Met Asp Arg Asp Ile Cys Leu Val Pro Ala Ser Ala His Gly Thr Asn 580 585 590 gcg gcg tcg gcg acg ctg gcg cac ctg cgg gtc gtg gtg gtc ggc acc 2124 Ala Ala Ser Ala Thr Leu Ala His Leu Arg Val Val Val Val Gly Thr 595 600 605 gcc ggg gac ggc tcc atc gac atg gac gac ctc gac gcg aaa ctc gcc 2172 Ala Gly Asp Gly Ser Ile Asp Met Asp Asp Leu Asp Ala Lys Leu Ala 610 615 620 gag cac ggc ccg cag gtg gcc gcg atc atg atc acc tac ccc tcc acg 2220 Glu His Gly Pro Gln Val Ala Ala Ile Met Ile Thr Tyr Pro Ser Thr 625 630 635 640 cac ggc gtc ttc gag gac acc gtg cgc gag gtg tgc gac agg gtg cac 2268 His Gly Val Phe Glu Asp Thr Val Arg Glu Val Cys Asp Arg Val His 645 650 655 gcg gtc ggc ggg cag gtc tac atc gac ggc gcg aac ctc aac gcc ctg 2316 Ala Val Gly Gly Gln Val Tyr Ile Asp Gly Ala Asn Leu Asn Ala Leu 660 665 670 acc ggc ctg gcg ctg ctg ggc cgc atc ggc ggc gac gtc tcc cac ctc 2364 Thr Gly Leu Ala Leu Leu Gly Arg Ile Gly Gly Asp Val Ser His Leu 675 680 685 aac ctg cac aag acg ttc acc atc ccg cac ggc ggc ggt ggc ccg ggc 2412 Asn Leu His Lys Thr Phe Thr Ile Pro His Gly Gly Gly Gly Pro Gly 690 695 700 gtg ggc ccg gtg tgc gtc gcg gag cac ctc gtc ccc ttc ctg ccg gcg 2460 Val Gly Pro Val Cys Val Ala Glu His Leu Val Pro Phe Leu Pro Ala 705 710 715 720 gac ccg acc tcc ctc gac ggc gcc acc ccg gcg gcg gtc ggc acc ggc 2508 Asp Pro Thr Ser Leu Asp Gly Ala Thr Pro Ala Ala Val Gly Thr Gly 725 730 735 gtg ccc gtc tct gcg acg cgc cac ggc tcg gcg ggc gtc ctg cag atc 2556 Val Pro Val Ser Ala Thr Arg His Gly Ser Ala Gly Val Leu Gln Ile 740 745 750 tcc tgg gcg tac atc gcc atg atg ggc acc gac ggc ctc acc cgc gcc 2604 Ser Trp Ala Tyr Ile Ala Met Met Gly Thr Asp Gly Leu Thr Arg Ala 755 760 765 agc gcc gcg gcg atc ctc agc gcg aac tac gtc gcc cac cgc ctg ggc 2652 Ser Ala Ala Ala Ile Leu Ser Ala Asn Tyr Val Ala His Arg Leu Gly 770 775 780 gag gcc ttc ccg gtg ctc tac acc ggc cgc aac ggc ctg gtg gcg cac 2700 Glu Ala Phe Pro Val Leu Tyr Thr Gly Arg Asn Gly Leu Val Ala His 785 790 795 800 gag tgc atc ctc gac ctg cgc gac ctg acg agg cag acc ggc gtc acc 2748 Glu Cys Ile Leu Asp Leu Arg Asp Leu Thr Arg Gln Thr Gly Val Thr 805 810 815 gcc acg gac gtg gcc aaa cgc ctc atc gac ttc ggc ttc cac gcc ccc 2796 Ala Thr Asp Val Ala Lys Arg Leu Ile Asp Phe Gly Phe His Ala Pro 820 825 830 acg ctc tcc ttc ccc gtc gcc ggt acc ctc atg gtc gag ccg acc gag 2844 Thr Leu Ser Phe Pro Val Ala Gly Thr Leu Met Val Glu Pro Thr Glu 835 840 845 tcc gag gac ctc gcc gag ctc gac cgc ttc atc gag gcg atg ctc acc 2892 Ser Glu Asp Leu Ala Glu Leu Asp Arg Phe Ile Glu Ala Met Leu Thr 850 855 860 atc cgc gcg gag atc gac gag atc agc ggc ggc gac atc gcc tac gag 2940 Ile Arg Ala Glu Ile Asp Glu Ile Ser Gly Gly Asp Ile Ala Tyr Glu 865 870 875 880 gac tcc gtc ctc cac cac gcg ccg ttc acc gcg tgg agc gtg tcc cgc 2988 Asp Ser Val Leu His His Ala Pro Phe Thr Ala Trp Ser Val Ser Arg 885 890 895 gac gac tgg gac cac gcc ttc acc cgt gag cag gcg gcc tgg ccg gtc 3036 Asp Asp Trp Asp His Ala Phe Thr Arg Glu Gln Ala Ala Trp Pro Val 900 905 910 ccc ggt ctg cgc cgc acc aag tac ttc ccg ccg gtc cgc cgc ctc aac 3084 Pro Gly Leu Arg Arg Thr Lys Tyr Phe Pro Pro Val Arg Arg Leu Asn 915 920 925 gag gcc tac ggt gac cgc cac ctc gtg tgc gcc tgc ccg ccg ccc gag 3132 Glu Ala Tyr Gly Asp Arg His Leu Val Cys Ala Cys Pro Pro Pro Glu 930 935 940 gcc ttc gac ctt tcc gag gag aac cag tga ccgacctgcg taccagcccc 3182 Ala Phe Asp Leu Ser Glu Glu Asn Gln 945 950 ctccacgagc gtcatgtgga gctcggggcc tccttcaccc ccttcggccc gtggaacatg 3242 cccctgcgct acggcaacga gctcgacgag caccgcgccg tccgttccac cgccgggctc 3302 ttcgacctct cgcacatggg tgagatccgc gtgtggggcg cacaggccgc ggagttcctc 3362 gaccatgcgc tgctctccgc cttctccacg ctccccgtgg gtaaggcgcg gtactccatg 3422 cttgtcgacg ccaccggtgc catcctcgac gacctcctca 3462 <210> 40 <211> 953 <212> PRT <213> Corynebacterium humireducens <400> 40 Met Ala Leu His Pro Ile Thr Asp Ser Ala Ser Tyr Leu Ala Arg His 1 5 10 15 Leu Gly Pro Asp Thr Ala Glu Glu Gln Val Met Leu Gly Ala Leu Gly 20 25 30 Tyr Gly Ser Leu Ala Glu Leu Val Asp Ala Ala Leu Pro Thr Asp Ile 35 40 45 Arg Leu Thr Glu Pro Leu Pro Arg His Ala Pro Leu Ser Glu Thr Glu 50 55 60 Ala Gln Ala Arg Leu Arg Glu Tyr Ala Ser Arg Asn Thr Val Leu Arg 65 70 75 80 Ser Phe Tyr Gly Gln Gly Phe Ser Asp Thr Val Thr Pro Pro Val Ile 85 90 95 Arg Arg Gly Val Leu Glu Ala Pro Gly Trp Tyr Thr Ala Tyr Thr Pro 100 105 110 Tyr Gln Pro Glu Ile Ser Gln Gly Arg Leu Glu Ala Leu Leu Asn Phe 115 120 125 Gln Thr Met Val Ser Glu Leu Thr Gly Leu Pro Val Ala Asn Ala Ser 130 135 140 Leu Leu Asp Glu Ala Ser Ala Thr Ala Glu Ala Val Gly Leu Met Ser 145 150 155 160 Arg Ala Gln Arg Lys Gly Arg Arg Val Leu Leu Asp Ala Arg Leu His 165 170 175 Pro Gln Val Leu Ala Val Ala Ala Glu Arg Ala Arg Ala Ile Asp Leu 180 185 190 Glu Val Ala Val Glu Asp Leu Ser Gly Pro Leu Val Gly Glu Asp Leu 195 200 205 Cys Gly Val Val Ile Ala Tyr Pro Gly Thr Glu Gly Asp Ile Ala Asp 210 215 220 Pro Arg Pro Val Ile Glu Ala Val His Ala Arg Gly Gly Leu Ala Thr 225 230 235 240 Val Val Thr Asp Pro Leu Ser Leu Leu Leu Leu Glu Ser Pro Gly Glu 245 250 255 Met Gly Ala Asp Ile Ala Val Gly Ser Ser Gln Arg Phe Gly Val Pro 260 265 270 Leu Phe Tyr Gly Gly Pro His Ala Ala Tyr Met Ala Val Ser Asp Ala 275 280 285 Leu Lys Arg Gln Ile Pro Gly Arg Leu Val Gly Val Ser Val Asp Ala 290 295 300 Glu Gly Ala Pro Ala Tyr Arg Leu Ala Leu Gln Thr Arg Glu Gln His 305 310 315 320 Ile Arg Arg Glu Arg Ala Thr Ser Asn Ile Cys Thr Ala Gln Ala Leu 325 330 335 Leu Ala Val Thr Ala Gly Met Tyr Ala Val Tyr His Gly Pro Glu Gly 340 345 350 Leu Arg Ala Ile Ala Glu Arg Val His Gly Leu Ala Cys Asp Phe Ala 355 360 365 Ala Ala Val Arg Ala Ala Gly Gly Thr Val Leu His Asp Ala Phe Phe 370 375 380 Asp Thr Val Ala Val Arg Val Glu Ala Ala Arg Pro Ile Val Gly Thr 385 390 395 400 Leu Ala Glu Ala Gly Tyr Leu Val Arg Ala Val Asp Gly Thr Thr Val 405 410 415 Gly Val Ser Phe Gly Glu Ser Ala Glu Glu Gly Asp Val Glu Lys Leu 420 425 430 Ala Ala Val Phe Gly Ala Ser Leu Thr Ala Gly Thr Arg Cys Ile Pro 435 440 445 Ala Ser Val Ala Arg Gln Thr Pro Thr Leu Thr His Pro Val Phe Ser 450 455 460 Thr Leu His Ser Glu Thr Gln Met Met Arg Tyr Leu Arg Thr Leu Ser 465 470 475 480 Asp Arg Asp Leu Ala Leu Asp Arg Thr Met Ile Pro Leu Gly Ser Cys 485 490 495 Thr Met Lys Leu Asn Pro Thr Ala Gly Leu Glu Thr Ile Thr Trp Pro 500 505 510 Glu Phe Ala Asp Met His Pro Phe Ala Pro Asp Asp Gln Ala Ala Gly 515 520 525 Trp Leu Ala Leu Ile Ala Glu Leu Glu Gly Trp Leu Ala Glu Leu Thr 530 535 540 Gly Tyr Ala Lys Val Ser Val Gln Pro Asn Ala Gly Ser Gln Gly Glu 545 550 555 560 Leu Ala Gly Leu Leu Ala Ile Arg Arg Tyr His Val Ala Asn Gly Asp 565 570 575 Met Asp Arg Asp Ile Cys Leu Val Pro Ala Ser Ala His Gly Thr Asn 580 585 590 Ala Ala Ser Ala Thr Leu Ala His Leu Arg Val Val Val Val Gly Thr 595 600 605 Ala Gly Asp Gly Ser Ile Asp Met Asp Asp Leu Asp Ala Lys Leu Ala 610 615 620 Glu His Gly Pro Gln Val Ala Ala Ile Met Ile Thr Tyr Pro Ser Thr 625 630 635 640 His Gly Val Phe Glu Asp Thr Val Arg Glu Val Cys Asp Arg Val His 645 650 655 Ala Val Gly Gly Gln Val Tyr Ile Asp Gly Ala Asn Leu Asn Ala Leu 660 665 670 Thr Gly Leu Ala Leu Leu Gly Arg Ile Gly Gly Asp Val Ser His Leu 675 680 685 Asn Leu His Lys Thr Phe Thr Ile Pro His Gly Gly Gly Gly Pro Gly 690 695 700 Val Gly Pro Val Cys Val Ala Glu His Leu Val Pro Phe Leu Pro Ala 705 710 715 720 Asp Pro Thr Ser Leu Asp Gly Ala Thr Pro Ala Ala Val Gly Thr Gly 725 730 735 Val Pro Val Ser Ala Thr Arg His Gly Ser Ala Gly Val Leu Gln Ile 740 745 750 Ser Trp Ala Tyr Ile Ala Met Met Gly Thr Asp Gly Leu Thr Arg Ala 755 760 765 Ser Ala Ala Ala Ile Leu Ser Ala Asn Tyr Val Ala His Arg Leu Gly 770 775 780 Glu Ala Phe Pro Val Leu Tyr Thr Gly Arg Asn Gly Leu Val Ala His 785 790 795 800 Glu Cys Ile Leu Asp Leu Arg Asp Leu Thr Arg Gln Thr Gly Val Thr 805 810 815 Ala Thr Asp Val Ala Lys Arg Leu Ile Asp Phe Gly Phe His Ala Pro 820 825 830 Thr Leu Ser Phe Pro Val Ala Gly Thr Leu Met Val Glu Pro Thr Glu 835 840 845 Ser Glu Asp Leu Ala Glu Leu Asp Arg Phe Ile Glu Ala Met Leu Thr 850 855 860 Ile Arg Ala Glu Ile Asp Glu Ile Ser Gly Gly Asp Ile Ala Tyr Glu 865 870 875 880 Asp Ser Val Leu His His Ala Pro Phe Thr Ala Trp Ser Val Ser Arg 885 890 895 Asp Asp Trp Asp His Ala Phe Thr Arg Glu Gln Ala Ala Trp Pro Val 900 905 910 Pro Gly Leu Arg Arg Thr Lys Tyr Phe Pro Pro Val Arg Arg Leu Asn 915 920 925 Glu Ala Tyr Gly Asp Arg His Leu Val Cys Ala Cys Pro Pro Pro Glu 930 935 940 Ala Phe Asp Leu Ser Glu Glu Asn Gln 945 950 <210> 41 <211> 1704 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1404) <223> gene gcvT <400> 41 cgagctcgac cgcttcatcg aggcgatgct caccatccgc gcggagatcg acgagatcag 60 cggcggcgac atcgcctacg aggactccgt cctccaccac gcgccgttca ccgcgtggag 120 cgtgtcccgc gacgactggg accacgcctt cacccgtgag caggcggcct ggccggtccc 180 cggtctgcgc cgcaccaagt acttcccgcc ggtccgccgc ctcaacgagg cctacggtga 240 ccgccacctc gtgtgcgcct gcccgccgcc cgaggccttc gacctttccg aggagaacca 300 gtg acc gac ctg cgt acc agc ccc ctc cac gag cgt cat gtg gag ctc 348 Val Thr Asp Leu Arg Thr Ser Pro Leu His Glu Arg His Val Glu Leu 1 5 10 15 ggg gcc tcc ttc acc ccc ttc ggc ccg tgg aac atg ccc ctg cgc tac 396 Gly Ala Ser Phe Thr Pro Phe Gly Pro Trp Asn Met Pro Leu Arg Tyr 20 25 30 ggc aac gag ctc gac gag cac cgc gcc gtc cgt tcc acc gcc ggg ctc 444 Gly Asn Glu Leu Asp Glu His Arg Ala Val Arg Ser Thr Ala Gly Leu 35 40 45 ttc gac ctc tcg cac atg ggt gag atc cgc gtg tgg ggc gca cag gcc 492 Phe Asp Leu Ser His Met Gly Glu Ile Arg Val Trp Gly Ala Gln Ala 50 55 60 gcg gag ttc ctc gac cat gcg ctg ctc tcc gcc ttc tcc acg ctc ccc 540 Ala Glu Phe Leu Asp His Ala Leu Leu Ser Ala Phe Ser Thr Leu Pro 65 70 75 80 gtg ggt aag gcg cgg tac tcc atg ctt gtc gac gcc acc ggt gcc atc 588 Val Gly Lys Ala Arg Tyr Ser Met Leu Val Asp Ala Thr Gly Ala Ile 85 90 95 ctc gac gac ctc ctc acc tac cgc ctc gcc ggg gac gag ttc ctg gtg 636 Leu Asp Asp Leu Leu Thr Tyr Arg Leu Ala Gly Asp Glu Phe Leu Val 100 105 110 gtc ccc aac gcg gcg aac acg gag acc gtg tgg gag gag ttc cgg gcc 684 Val Pro Asn Ala Ala Asn Thr Glu Thr Val Trp Glu Glu Phe Arg Ala 115 120 125 cgc gcc gcc ggc ttc gac gtg gag ctg gcc gac gag tcc gcc gac act 732 Arg Ala Ala Gly Phe Asp Val Glu Leu Ala Asp Glu Ser Ala Asp Thr 130 135 140 gcg ctg ctg gct gtg cag ggc ccc cgc gcc gag cag atc atc gcc gac 780 Ala Leu Leu Ala Val Gln Gly Pro Arg Ala Glu Gln Ile Ile Ala Asp 145 150 155 160 ctc gtc gcc ggg gac gac gag tcg gtc gtg cgc acg atg aag ttc tac 828 Leu Val Ala Gly Asp Asp Glu Ser Val Val Arg Thr Met Lys Phe Tyr 165 170 175 tcg gcg gcc ccg ttg acg gtg gcg ggc gtc gat acg ctg ctc gcc cgc 876 Ser Ala Ala Pro Leu Thr Val Ala Gly Val Asp Thr Leu Leu Ala Arg 180 185 190 acc ggc tac acc ggg gag gac ggc ttc gag ctc tac gtc tcc ggc gcg 924 Thr Gly Tyr Thr Gly Glu Asp Gly Phe Glu Leu Tyr Val Ser Gly Ala 195 200 205 gac gcc gct gaa ctg tgg gac gcg ctg ctc aac gcc ggc gcc gcc tac 972 Asp Ala Ala Glu Leu Trp Asp Ala Leu Leu Asn Ala Gly Ala Ala Tyr 210 215 220 gac atg ctg ccc gcc gga ctg gcg gcg cgg gac tcc ctg cgc ctg gag 1020 Asp Met Leu Pro Ala Gly Leu Ala Ala Arg Asp Ser Leu Arg Leu Glu 225 230 235 240 gcc ggg ctc ccc ctc tac ggg cag gag ctc acc cgc gac atc acg ccg 1068 Ala Gly Leu Pro Leu Tyr Gly Gln Glu Leu Thr Arg Asp Ile Thr Pro 245 250 255 gtg gag gcc ggg atg gcc cgc gcc ttc gcc tcg aag gag ggg gac ttc 1116 Val Glu Ala Gly Met Ala Arg Ala Phe Ala Ser Lys Glu Gly Asp Phe 260 265 270 gtc ggc cgc gac gcc ctc gtc ggc agg gag ccg acc agc gcc atc gtc 1164 Val Gly Arg Asp Ala Leu Val Gly Arg Glu Pro Thr Ser Ala Ile Val 275 280 285 ggg ctc cgg ggt ctg ggg cgc cgc gcg gcc cgc gag ggc gcc gag gtc 1212 Gly Leu Arg Gly Leu Gly Arg Arg Ala Ala Arg Glu Gly Ala Glu Val 290 295 300 tac ctc gac ggc gaa cag gtc ggc gtg gtc acc tcc ggg cag ccg gcg 1260 Tyr Leu Asp Gly Glu Gln Val Gly Val Val Thr Ser Gly Gln Pro Ala 305 310 315 320 ccg gtg ctc ggc tac ccg atc gca ctc gcc cgc ctg cgc ccc gac gtc 1308 Pro Val Leu Gly Tyr Pro Ile Ala Leu Ala Arg Leu Arg Pro Asp Val 325 330 335 acc gcg gcc ggc acg gca ctg gag gtg gac atc cgt ggg agg cgg cac 1356 Thr Ala Ala Gly Thr Ala Leu Glu Val Asp Ile Arg Gly Arg Arg His 340 345 350 ccc ttc gag atc gtc gac ctg ccc ttc tac cgc cgc gcc gca cga taa 1404 Pro Phe Glu Ile Val Asp Leu Pro Phe Tyr Arg Arg Ala Ala Arg 355 360 365 cctgaaccca ccaccaacgg aaaggctccc ccaccatggc caccgagcac tccctgcccg 1464 ggaactactc ctactccgag gaccacgagt ggatcgacgc gacgcccgag gacgccgtcg 1524 gcgcgaccgt gaaggtcggc atcacctccg tggccaccga acgcctcggc gaggtcgtct 1584 acgcggagct cccgcaggtc ggtgacgccg tcaccgccgg tgaaccgtgc ggcgaggtcg 1644 agtccacgaa gtcggtctcc gacctctacg cgccggtcac gggcaccgtc accgaggtca 1704 <210> 42 <211> 367 <212> PRT <213> Corynebacterium humireducens <400> 42 Val Thr Asp Leu Arg Thr Ser Pro Leu His Glu Arg His Val Glu Leu 1 5 10 15 Gly Ala Ser Phe Thr Pro Phe Gly Pro Trp Asn Met Pro Leu Arg Tyr 20 25 30 Gly Asn Glu Leu Asp Glu His Arg Ala Val Arg Ser Thr Ala Gly Leu 35 40 45 Phe Asp Leu Ser His Met Gly Glu Ile Arg Val Trp Gly Ala Gln Ala 50 55 60 Ala Glu Phe Leu Asp His Ala Leu Leu Ser Ala Phe Ser Thr Leu Pro 65 70 75 80 Val Gly Lys Ala Arg Tyr Ser Met Leu Val Asp Ala Thr Gly Ala Ile 85 90 95 Leu Asp Asp Leu Leu Thr Tyr Arg Leu Ala Gly Asp Glu Phe Leu Val 100 105 110 Val Pro Asn Ala Ala Asn Thr Glu Thr Val Trp Glu Glu Phe Arg Ala 115 120 125 Arg Ala Ala Gly Phe Asp Val Glu Leu Ala Asp Glu Ser Ala Asp Thr 130 135 140 Ala Leu Leu Ala Val Gln Gly Pro Arg Ala Glu Gln Ile Ile Ala Asp 145 150 155 160 Leu Val Ala Gly Asp Asp Glu Ser Val Val Arg Thr Met Lys Phe Tyr 165 170 175 Ser Ala Ala Pro Leu Thr Val Ala Gly Val Asp Thr Leu Leu Ala Arg 180 185 190 Thr Gly Tyr Thr Gly Glu Asp Gly Phe Glu Leu Tyr Val Ser Gly Ala 195 200 205 Asp Ala Ala Glu Leu Trp Asp Ala Leu Leu Asn Ala Gly Ala Ala Tyr 210 215 220 Asp Met Leu Pro Ala Gly Leu Ala Ala Arg Asp Ser Leu Arg Leu Glu 225 230 235 240 Ala Gly Leu Pro Leu Tyr Gly Gln Glu Leu Thr Arg Asp Ile Thr Pro 245 250 255 Val Glu Ala Gly Met Ala Arg Ala Phe Ala Ser Lys Glu Gly Asp Phe 260 265 270 Val Gly Arg Asp Ala Leu Val Gly Arg Glu Pro Thr Ser Ala Ile Val 275 280 285 Gly Leu Arg Gly Leu Gly Arg Arg Ala Ala Arg Glu Gly Ala Glu Val 290 295 300 Tyr Leu Asp Gly Glu Gln Val Gly Val Val Thr Ser Gly Gln Pro Ala 305 310 315 320 Pro Val Leu Gly Tyr Pro Ile Ala Leu Ala Arg Leu Arg Pro Asp Val 325 330 335 Thr Ala Ala Gly Thr Ala Leu Glu Val Asp Ile Arg Gly Arg Arg His 340 345 350 Pro Phe Glu Ile Val Asp Leu Pro Phe Tyr Arg Arg Ala Ala Arg 355 360 365 <210> 43 <211> 1899 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1599) <223> gene glyA <400> 43 gacaggggga gatagacctc ggcgacctcc acgaggtcga tcgattcgcc gatgccgcgg 60 agctgctcga cctcctcctc cgtgagcacc tgcggcatcg attgacgcag ggcacgccag 120 gaggagcggt cgaaatccac gtagggggtg gcgtccttgg gccgagacat gcgtcccatt 180 gtgtcatctg ccccgtcaac tgtgtcccac tcacccgggg tgcgggggta tgaggtgccc 240 tgtgggcggt atgctgtcca cgaatgccgt gtacccctag aaaggctggc tgtaacgccc 300 atg acc gct gag tct tct gat ctc cgt tac cag gaa ctg tcc gtc ctc 348 Met Thr Ala Glu Ser Ser Asp Leu Arg Tyr Gln Glu Leu Ser Val Leu 1 5 10 15 gac ccg gag gtc cac gcc gcc atc ggc ggt gag atc gcc cgt cag cgt 396 Asp Pro Glu Val His Ala Ala Ile Gly Gly Glu Ile Ala Arg Gln Arg 20 25 30 gac ttc ctc gag atg atc gcg tcc gag aac ttc gtc ccc cgt gcc gtc 444 Asp Phe Leu Glu Met Ile Ala Ser Glu Asn Phe Val Pro Arg Ala Val 35 40 45 ctg cag gca cag ggt tcg gtg ttc acc aac aag tac gcc gag ggc tac 492 Leu Gln Ala Gln Gly Ser Val Phe Thr Asn Lys Tyr Ala Glu Gly Tyr 50 55 60 ccg ggg cgt cgt tac tac ggt ggc tgc gag aac gcc gac atc gtc gag 540 Pro Gly Arg Arg Tyr Tyr Gly Gly Cys Glu Asn Ala Asp Ile Val Glu 65 70 75 80 gac ctg gcc cgc gac cgc gcc aag gcc ctg ttc ggc gcc gag ttc gcc 588 Asp Leu Ala Arg Asp Arg Ala Lys Ala Leu Phe Gly Ala Glu Phe Ala 85 90 95 aac gtc cag ccg cac tcc ggc gcc cag gcc aac gcc gcc gtc ctg cac 636 Asn Val Gln Pro His Ser Gly Ala Gln Ala Asn Ala Ala Val Leu His 100 105 110 gcg ctg gcc gac gcc ggc gac aag atc atg ggt ctg tcc ctg gcc cac 684 Ala Leu Ala Asp Ala Gly Asp Lys Ile Met Gly Leu Ser Leu Ala His 115 120 125 ggt ggt cac ctg acc cac ggc atg aag ctc aac ttc tcc ggc aag ctc 732 Gly Gly His Leu Thr His Gly Met Lys Leu Asn Phe Ser Gly Lys Leu 130 135 140 tac gac gtc gtc gcc tac gag gtc gac ccg gag acc cac cgc atc gac 780 Tyr Asp Val Val Ala Tyr Glu Val Asp Pro Glu Thr His Arg Ile Asp 145 150 155 160 atg gac cgc gtc cgc gag cag gcc ctg gcc gag cgc ccg aag gtc ctc 828 Met Asp Arg Val Arg Glu Gln Ala Leu Ala Glu Arg Pro Lys Val Leu 165 170 175 atc gcc ggc tgg tcc gcg tac ccg cgc acc ctc gac ttc gag gcg ttc 876 Ile Ala Gly Trp Ser Ala Tyr Pro Arg Thr Leu Asp Phe Glu Ala Phe 180 185 190 cgc tcc atc gcc gac gag gtc ggc gcg tac ctg tgg acc gac atg gcg 924 Arg Ser Ile Ala Asp Glu Val Gly Ala Tyr Leu Trp Thr Asp Met Ala 195 200 205 cac ttc gcc ggc ctc gtc gcc gcc gac ctg cac ccg tcc ccg gtc ccg 972 His Phe Ala Gly Leu Val Ala Ala Asp Leu His Pro Ser Pro Val Pro 210 215 220 cac tcc gac gtc gtc tcc acc acc gtc cac aag acg ctg ggt ggc ccg 1020 His Ser Asp Val Val Ser Thr Thr Val His Lys Thr Leu Gly Gly Pro 225 230 235 240 cgt tcc ggc ctg atc gtc gcg aag cag gag tgg gcc aag aag ctc aac 1068 Arg Ser Gly Leu Ile Val Ala Lys Gln Glu Trp Ala Lys Lys Leu Asn 245 250 255 tcc gcc gtc ttc ccg ggc cag cag ggt ggt ccg ctc atg cac gcc atc 1116 Ser Ala Val Phe Pro Gly Gln Gln Gly Gly Pro Leu Met His Ala Ile 260 265 270 gcc gcc aag gcc gtg gca ctg aag gtc gcc ggc acc gac gag ttc aag 1164 Ala Ala Lys Ala Val Ala Leu Lys Val Ala Gly Thr Asp Glu Phe Lys 275 280 285 gac cgt cag gcc cgc acc atc gag ggt gcc aag atc ctg gcc gag cgt 1212 Asp Arg Gln Ala Arg Thr Ile Glu Gly Ala Lys Ile Leu Ala Glu Arg 290 295 300 ctc acc gcc gct gac gcg aag gcc gcc ggc atc gac gtc ctc acc ggt 1260 Leu Thr Ala Ala Asp Ala Lys Ala Ala Gly Ile Asp Val Leu Thr Gly 305 310 315 320 ggc acc gac gtg cac ctg gtc ctc gct gac ctg cgc aac tcc gag atg 1308 Gly Thr Asp Val His Leu Val Leu Ala Asp Leu Arg Asn Ser Glu Met 325 330 335 gac ggc cag cag gcc gaa gac ctc ctc cac gag gtc ggc atc acc gtc 1356 Asp Gly Gln Gln Ala Glu Asp Leu Leu His Glu Val Gly Ile Thr Val 340 345 350 aac cgc aac gcc gtc ccg aac gac ccg cgc ccg ccg atg gtc acc tcc 1404 Asn Arg Asn Ala Val Pro Asn Asp Pro Arg Pro Pro Met Val Thr Ser 355 360 365 ggc ctg cgc atc ggc acc ccg gcc ctg gcc acc cgt ggt ttc gac gcc 1452 Gly Leu Arg Ile Gly Thr Pro Ala Leu Ala Thr Arg Gly Phe Asp Ala 370 375 380 gcc gcc ttc acc gag gtc gcc gac atc atc ggc acc gcc ctg gtc aac 1500 Ala Ala Phe Thr Glu Val Ala Asp Ile Ile Gly Thr Ala Leu Val Asn 385 390 395 400 ggc aag aac gcc gac atc gag gca ctg cgt gcc cgc gtc gcg aag ctg 1548 Gly Lys Asn Ala Asp Ile Glu Ala Leu Arg Ala Arg Val Ala Lys Leu 405 410 415 acc gac cag tac ccg ctg tac gag ggt ctg gag gag tgg aag atc aac 1596 Thr Asp Gln Tyr Pro Leu Tyr Glu Gly Leu Glu Glu Trp Lys Ile Asn 420 425 430 tag gtcttcccgc cctccccgtg cgcgtcctcg tcatagacaa cctcgattcc 1649 ttcacctgga atctcgtcgc ctacgtcgag gacgtcaccg gtgtcgcccc cgaggtgatc 1709 accaacaacg aacccggatg ggatgtcgac cgcgtcgcca cctacgacgc cgtcatcatc 1769 tccccgggcc ccggccgccc ggagcgtgag aaggacatcg gcatgtgcct cgatgtcctg 1829 cgcgacgggc gtgtcccgat tctgggggtc tgcctcgggc accaggccct cgcccacgcg 1889 tggggcggca 1899 <210> 44 <211> 432 <212> PRT <213> Corynebacterium humireducens <400> 44 Met Thr Ala Glu Ser Ser Asp Leu Arg Tyr Gln Glu Leu Ser Val Leu 1 5 10 15 Asp Pro Glu Val His Ala Ala Ile Gly Gly Glu Ile Ala Arg Gln Arg 20 25 30 Asp Phe Leu Glu Met Ile Ala Ser Glu Asn Phe Val Pro Arg Ala Val 35 40 45 Leu Gln Ala Gln Gly Ser Val Phe Thr Asn Lys Tyr Ala Glu Gly Tyr 50 55 60 Pro Gly Arg Arg Tyr Tyr Gly Gly Cys Glu Asn Ala Asp Ile Val Glu 65 70 75 80 Asp Leu Ala Arg Asp Arg Ala Lys Ala Leu Phe Gly Ala Glu Phe Ala 85 90 95 Asn Val Gln Pro His Ser Gly Ala Gln Ala Asn Ala Ala Val Leu His 100 105 110 Ala Leu Ala Asp Ala Gly Asp Lys Ile Met Gly Leu Ser Leu Ala His 115 120 125 Gly Gly His Leu Thr His Gly Met Lys Leu Asn Phe Ser Gly Lys Leu 130 135 140 Tyr Asp Val Val Ala Tyr Glu Val Asp Pro Glu Thr His Arg Ile Asp 145 150 155 160 Met Asp Arg Val Arg Glu Gln Ala Leu Ala Glu Arg Pro Lys Val Leu 165 170 175 Ile Ala Gly Trp Ser Ala Tyr Pro Arg Thr Leu Asp Phe Glu Ala Phe 180 185 190 Arg Ser Ile Ala Asp Glu Val Gly Ala Tyr Leu Trp Thr Asp Met Ala 195 200 205 His Phe Ala Gly Leu Val Ala Ala Asp Leu His Pro Ser Pro Val Pro 210 215 220 His Ser Asp Val Val Ser Thr Thr Val His Lys Thr Leu Gly Gly Pro 225 230 235 240 Arg Ser Gly Leu Ile Val Ala Lys Gln Glu Trp Ala Lys Lys Leu Asn 245 250 255 Ser Ala Val Phe Pro Gly Gln Gln Gly Gly Pro Leu Met His Ala Ile 260 265 270 Ala Ala Lys Ala Val Ala Leu Lys Val Ala Gly Thr Asp Glu Phe Lys 275 280 285 Asp Arg Gln Ala Arg Thr Ile Glu Gly Ala Lys Ile Leu Ala Glu Arg 290 295 300 Leu Thr Ala Ala Asp Ala Lys Ala Ala Gly Ile Asp Val Leu Thr Gly 305 310 315 320 Gly Thr Asp Val His Leu Val Leu Ala Asp Leu Arg Asn Ser Glu Met 325 330 335 Asp Gly Gln Gln Ala Glu Asp Leu Leu His Glu Val Gly Ile Thr Val 340 345 350 Asn Arg Asn Ala Val Pro Asn Asp Pro Arg Pro Pro Met Val Thr Ser 355 360 365 Gly Leu Arg Ile Gly Thr Pro Ala Leu Ala Thr Arg Gly Phe Asp Ala 370 375 380 Ala Ala Phe Thr Glu Val Ala Asp Ile Ile Gly Thr Ala Leu Val Asn 385 390 395 400 Gly Lys Asn Ala Asp Ile Glu Ala Leu Arg Ala Arg Val Ala Lys Leu 405 410 415 Thr Asp Gln Tyr Pro Leu Tyr Glu Gly Leu Glu Glu Trp Lys Ile Asn 420 425 430 <210> 45 <211> 1932 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1632) <223> gene hom <400> 45 gcggcgtgcg cggacgagat ccccgccagt gggatcacca ggcacgccac gcaggccgtg 60 agtgcacggg tgatccgacg gcgggacatg ctgactcctg tcactggcag aggcatgggg 120 gtggtgagga cagattaatc gtctgaacgc aatatgtcca gagatgtgac ttatcttatg 180 gagtgtcgtg accccggagt ctgaggaagt ggccgaatcc cggtagggtg gtcacggaaa 240 tctgtcctca gtctgcccgt ccccgacggg cacggaccca cgaccatgtg caggagaacc 300 atg acc gtc act cag cag ccc agc ttc aac ccc ggc aag gga gcc ggc 348 Met Thr Val Thr Gln Gln Pro Ser Phe Asn Pro Gly Lys Gly Ala Gly 1 5 10 15 gag ccg gtg ggc gtc gcc att ctc ggc cac ggc acc gtc gga tct gag 396 Glu Pro Val Gly Val Ala Ile Leu Gly His Gly Thr Val Gly Ser Glu 20 25 30 gtg ctg cgc ctg atg ggc gag tac tcc gac gcg ctc gcg cac cgc atc 444 Val Leu Arg Leu Met Gly Glu Tyr Ser Asp Ala Leu Ala His Arg Ile 35 40 45 ggc ggt ccg ctc gag ctc aag ggt gtg gcc gtg tcc gac ctc tcc aag 492 Gly Gly Pro Leu Glu Leu Lys Gly Val Ala Val Ser Asp Leu Ser Lys 50 55 60 ccg cgc gag ggc gtg gac cgt gag ctg ctc acc gat gac gcg ctg agc 540 Pro Arg Glu Gly Val Asp Arg Glu Leu Leu Thr Asp Asp Ala Leu Ser 65 70 75 80 ctc atc aac cgc gac gac gtc gac atc gtc gtc gag gtc atc ggc ggc 588 Leu Ile Asn Arg Asp Asp Val Asp Ile Val Val Glu Val Ile Gly Gly 85 90 95 atc gag tac ccg cgt gag ctg gtc ctg gcc gcc ctg cgc gcc ggc aag 636 Ile Glu Tyr Pro Arg Glu Leu Val Leu Ala Ala Leu Arg Ala Gly Lys 100 105 110 tcc gtc gtc acc gcc aac aag gcc ctc gtc gcc gcg cac gcc gac gag 684 Ser Val Val Thr Ala Asn Lys Ala Leu Val Ala Ala His Ala Asp Glu 115 120 125 ctt gcc gac gcc gcc gac gcc gcc aac gtc gac ctc tac ttc gag gcc 732 Leu Ala Asp Ala Ala Asp Ala Ala Asn Val Asp Leu Tyr Phe Glu Ala 130 135 140 gcc gtc gcc gcc gcc atc ccg gtc gtc ggc atg ctg cgt cgt tcc ctc 780 Ala Val Ala Ala Ala Ile Pro Val Val Gly Met Leu Arg Arg Ser Leu 145 150 155 160 gcc ggc gac aac atc ctg cgt atc tcc ggc atc gtc aac ggc acc acc 828 Ala Gly Asp Asn Ile Leu Arg Ile Ser Gly Ile Val Asn Gly Thr Thr 165 170 175 aac ttc atc ctc gac gcg atg gag tcc acc ggc gcc agc tac gac gac 876 Asn Phe Ile Leu Asp Ala Met Glu Ser Thr Gly Ala Ser Tyr Asp Asp 180 185 190 gcg ctc gcc gag gcc acc cgt ctg ggc tac gcc gag gcc gac ccg acc 924 Ala Leu Ala Glu Ala Thr Arg Leu Gly Tyr Ala Glu Ala Asp Pro Thr 195 200 205 gcc gac gtc gag ggc cac gac gcc gcc tcc aag gcc gcc atc ctc gcc 972 Ala Asp Val Glu Gly His Asp Ala Ala Ser Lys Ala Ala Ile Leu Ala 210 215 220 tcc ctg ggc ttc cac acc cgc gtc aag ttc gac gac gtc agc tgc gag 1020 Ser Leu Gly Phe His Thr Arg Val Lys Phe Asp Asp Val Ser Cys Glu 225 230 235 240 ggc atc tcc aag gtc acc gcc gag gac atc gag gcc gcc cgc gac gcc 1068 Gly Ile Ser Lys Val Thr Ala Glu Asp Ile Glu Ala Ala Arg Asp Ala 245 250 255 ggc tac gtg atc aag ctg ctc gcc atc tgt gag cgc gtc acc gac aag 1116 Gly Tyr Val Ile Lys Leu Leu Ala Ile Cys Glu Arg Val Thr Asp Lys 260 265 270 gac ggc gtg gag tcc gtc gcc agc cgc gtc cac ccg acc ctc gtt ccg 1164 Asp Gly Val Glu Ser Val Ala Ser Arg Val His Pro Thr Leu Val Pro 275 280 285 gtc gac cac ccg ctg gcc tcg gtc aac cag tcc tac aac gcc atc ttc 1212 Val Asp His Pro Leu Ala Ser Val Asn Gln Ser Tyr Asn Ala Ile Phe 290 295 300 gtc gag gcc gag gca gcc ggt cgc ctg atg ttc tac ggc aac ggt gcc 1260 Val Glu Ala Glu Ala Ala Gly Arg Leu Met Phe Tyr Gly Asn Gly Ala 305 310 315 320 ggt ggc gcc ccg acc gcc tcc gcc gtc ctc ggt gac ctc gtc ggc gcc 1308 Gly Gly Ala Pro Thr Ala Ser Ala Val Leu Gly Asp Leu Val Gly Ala 325 330 335 gcc cgc aac aag gtc cac ggt ggc cgc gcc ccg ggc gag aac acc tac 1356 Ala Arg Asn Lys Val His Gly Gly Arg Ala Pro Gly Glu Asn Thr Tyr 340 345 350 gcg aac ctg ccg atc gcc gac ctc ggc gac gtg cgc acc cgc tac cac 1404 Ala Asn Leu Pro Ile Ala Asp Leu Gly Asp Val Arg Thr Arg Tyr His 355 360 365 atc gac atg gag gtc gag gac cgc acc ggt gtc ctc gcc gag ctg tcc 1452 Ile Asp Met Glu Val Glu Asp Arg Thr Gly Val Leu Ala Glu Leu Ser 370 375 380 gcg atc ttc gcc acc cac gac atc tcc ctc aag acc gtc cgc cag gag 1500 Ala Ile Phe Ala Thr His Asp Ile Ser Leu Lys Thr Val Arg Gln Glu 385 390 395 400 gag gcc ggc gac cac gcc cgc ctc atc gtc gtc acg cat tcc gca cgt 1548 Glu Ala Gly Asp His Ala Arg Leu Ile Val Val Thr His Ser Ala Arg 405 410 415 gag gcc gac ctg gcc gcc acc gtc gag gac ctg aag aag gcc ccg gcg 1596 Glu Ala Asp Leu Ala Ala Thr Val Glu Asp Leu Lys Lys Ala Pro Ala 420 425 430 gtc aag tcc gtc gac agc gtc ctg cga ctc atc tag ggagcgttga 1642 Val Lys Ser Val Asp Ser Val Leu Arg Leu Ile 435 440 gctgtgagca tccagcttga ggtgggcacc cgggccaccg tcaccgtccc cggctcctcc 1702 gcgaacctgg gccccggatt cgacaccctc ggcctggccg tgggcatcta cgacaccgtc 1762 gaggtcgagg tcatcgagtc gggcctcgag gtcgaggtct tcggcgaggg cgaggacgac 1822 ctgccccgcg acggttccca cctggtggtc aaggccatcc ggtcgggcct caacgccgcc 1882 cacgccgagg ccccgggcct gcgcgtggtg tgcaccaacg ccatcccgca 1932 <210> 46 <211> 443 <212> PRT <213> Corynebacterium humireducens <400> 46 Met Thr Val Thr Gln Gln Pro Ser Phe Asn Pro Gly Lys Gly Ala Gly 1 5 10 15 Glu Pro Val Gly Val Ala Ile Leu Gly His Gly Thr Val Gly Ser Glu 20 25 30 Val Leu Arg Leu Met Gly Glu Tyr Ser Asp Ala Leu Ala His Arg Ile 35 40 45 Gly Gly Pro Leu Glu Leu Lys Gly Val Ala Val Ser Asp Leu Ser Lys 50 55 60 Pro Arg Glu Gly Val Asp Arg Glu Leu Leu Thr Asp Asp Ala Leu Ser 65 70 75 80 Leu Ile Asn Arg Asp Asp Val Asp Ile Val Val Glu Val Ile Gly Gly 85 90 95 Ile Glu Tyr Pro Arg Glu Leu Val Leu Ala Ala Leu Arg Ala Gly Lys 100 105 110 Ser Val Val Thr Ala Asn Lys Ala Leu Val Ala Ala His Ala Asp Glu 115 120 125 Leu Ala Asp Ala Ala Asp Ala Ala Asn Val Asp Leu Tyr Phe Glu Ala 130 135 140 Ala Val Ala Ala Ala Ile Pro Val Val Gly Met Leu Arg Arg Ser Leu 145 150 155 160 Ala Gly Asp Asn Ile Leu Arg Ile Ser Gly Ile Val Asn Gly Thr Thr 165 170 175 Asn Phe Ile Leu Asp Ala Met Glu Ser Thr Gly Ala Ser Tyr Asp Asp 180 185 190 Ala Leu Ala Glu Ala Thr Arg Leu Gly Tyr Ala Glu Ala Asp Pro Thr 195 200 205 Ala Asp Val Glu Gly His Asp Ala Ala Ser Lys Ala Ala Ile Leu Ala 210 215 220 Ser Leu Gly Phe His Thr Arg Val Lys Phe Asp Asp Val Ser Cys Glu 225 230 235 240 Gly Ile Ser Lys Val Thr Ala Glu Asp Ile Glu Ala Ala Arg Asp Ala 245 250 255 Gly Tyr Val Ile Lys Leu Leu Ala Ile Cys Glu Arg Val Thr Asp Lys 260 265 270 Asp Gly Val Glu Ser Val Ala Ser Arg Val His Pro Thr Leu Val Pro 275 280 285 Val Asp His Pro Leu Ala Ser Val Asn Gln Ser Tyr Asn Ala Ile Phe 290 295 300 Val Glu Ala Glu Ala Ala Gly Arg Leu Met Phe Tyr Gly Asn Gly Ala 305 310 315 320 Gly Gly Ala Pro Thr Ala Ser Ala Val Leu Gly Asp Leu Val Gly Ala 325 330 335 Ala Arg Asn Lys Val His Gly Gly Arg Ala Pro Gly Glu Asn Thr Tyr 340 345 350 Ala Asn Leu Pro Ile Ala Asp Leu Gly Asp Val Arg Thr Arg Tyr His 355 360 365 Ile Asp Met Glu Val Glu Asp Arg Thr Gly Val Leu Ala Glu Leu Ser 370 375 380 Ala Ile Phe Ala Thr His Asp Ile Ser Leu Lys Thr Val Arg Gln Glu 385 390 395 400 Glu Ala Gly Asp His Ala Arg Leu Ile Val Val Thr His Ser Ala Arg 405 410 415 Glu Ala Asp Leu Ala Ala Thr Val Glu Asp Leu Lys Lys Ala Pro Ala 420 425 430 Val Lys Ser Val Asp Ser Val Leu Arg Leu Ile 435 440 <210> 47 <211> 1644 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1344) <223> gene lipA <400> 47 gcggggtgac catgcacggg ctgagcctga actgctcgaa cacgctcgag cactacgggc 60 acatcgtggc gtgcggcatc gacgacgccg acgtcaccac cctcagcatg gaactggggc 120 gcgacgtcag cgtcggggag gtcacggcac cgcttgtcga cgccctcctg gccgccctcg 180 ccggtgaact cgccgtcgcg gaccacacct tcggatcggc gcccgatccg acgaagattc 240 ccgcgaaaac gcgctgacct ctaagtttat ccctatcagt agaaagagta tggtggcgtt 300 gtg act atc gca cct gaa gga cgc aag ctg ctc cgg gtg gag aag cgc 348 Val Thr Ile Ala Pro Glu Gly Arg Lys Leu Leu Arg Val Glu Lys Arg 1 5 10 15 aat gcg cag acc ccc atc gag acg aag ccc cgt tgg atc cgc aac gcg 396 Asn Ala Gln Thr Pro Ile Glu Thr Lys Pro Arg Trp Ile Arg Asn Ala 20 25 30 gtg aag acc ggc ccc gag tac gag gac atg aag aag aag gtg gcg ggc 444 Val Lys Thr Gly Pro Glu Tyr Glu Asp Met Lys Lys Lys Val Ala Gly 35 40 45 gcc tcc ctg cac acc gtc tgt cag gag gcg ggc tgc ccc aac atc cac 492 Ala Ser Leu His Thr Val Cys Gln Glu Ala Gly Cys Pro Asn Ile His 50 55 60 gag tgc tgg gag tcc cgc gag gcg acg ttt ctc atc ggc ggc gcc aac 540 Glu Cys Trp Glu Ser Arg Glu Ala Thr Phe Leu Ile Gly Gly Ala Asn 65 70 75 80 tgc tcc cgc cgc tgc gac ttc tgc cag atc aac tcc gcg aag ccg gag 588 Cys Ser Arg Arg Cys Asp Phe Cys Gln Ile Asn Ser Ala Lys Pro Glu 85 90 95 ccg ctg gac cgc gac gag ccg cgc cgc gtg gcc gag tcc gtc cgc gag 636 Pro Leu Asp Arg Asp Glu Pro Arg Arg Val Ala Glu Ser Val Arg Glu 100 105 110 atg ggc ctg aac tac tcc acc atc acc ggc gtg acc cgc gac gac ctc 684 Met Gly Leu Asn Tyr Ser Thr Ile Thr Gly Val Thr Arg Asp Asp Leu 115 120 125 gag gac gag ggt gcc tgg ctc tac gcg gag gtc gtc cgc cag atc cac 732 Glu Asp Glu Gly Ala Trp Leu Tyr Ala Glu Val Val Arg Gln Ile His 130 135 140 gag ctc aac ccg aac acc ggc gtc gag aac ctc gtg ccc gac ttc tcc 780 Glu Leu Asn Pro Asn Thr Gly Val Glu Asn Leu Val Pro Asp Phe Ser 145 150 155 160 ggc cgt tcc gag ctg ctg gag cag gtc ttc gag tcc cgc ccc gag gtg 828 Gly Arg Ser Glu Leu Leu Glu Gln Val Phe Glu Ser Arg Pro Glu Val 165 170 175 ttc gcc cac aac ctg gag acc gtg ccg cgc atc ttc aag cgc atc cgc 876 Phe Ala His Asn Leu Glu Thr Val Pro Arg Ile Phe Lys Arg Ile Arg 180 185 190 ccg gcc ttc cgc tac gag cgt tcc ctc gac gtg atc cgc cag gcc cac 924 Pro Ala Phe Arg Tyr Glu Arg Ser Leu Asp Val Ile Arg Gln Ala His 195 200 205 gac tac ggg ctg atc acc aag tcg aac ctc atc ctc ggc atg ggc gag 972 Asp Tyr Gly Leu Ile Thr Lys Ser Asn Leu Ile Leu Gly Met Gly Glu 210 215 220 acc ctc gac gag atc cgt gag gcc ctc acc gac ctg cac agc gcc ggc 1020 Thr Leu Asp Glu Ile Arg Glu Ala Leu Thr Asp Leu His Ser Ala Gly 225 230 235 240 tgc gac atc atc acc atc acg cag tac ctg cgc ccc ggc ccg atg tac 1068 Cys Asp Ile Ile Thr Ile Thr Gln Tyr Leu Arg Pro Gly Pro Met Tyr 245 250 255 cac ccg atc gac cgc tgg gtg aag ccg gag gag ttc gtc gag cac aag 1116 His Pro Ile Asp Arg Trp Val Lys Pro Glu Glu Phe Val Glu His Lys 260 265 270 gac ttc gcc gag gag ctc ggc ttc ggt gcc gtc atg tcc ggc ccg ctg 1164 Asp Phe Ala Glu Glu Leu Gly Phe Gly Ala Val Met Ser Gly Pro Leu 275 280 285 gtc cgt tcc tcc tac cgt gcg gga cgt ctc tac tcg cag gcg atg gcc 1212 Val Arg Ser Ser Tyr Arg Ala Gly Arg Leu Tyr Ser Gln Ala Met Ala 290 295 300 gcc cgc ggc cgg gag atc ccc gac cac ctc aag cac ctg gcc gag acc 1260 Ala Arg Gly Arg Glu Ile Pro Asp His Leu Lys His Leu Ala Glu Thr 305 310 315 320 tcg cag ggc agc acc gac cag gag gcc acc acc ctc ctg gcc aag tac 1308 Ser Gln Gly Ser Thr Asp Gln Glu Ala Thr Thr Leu Leu Ala Lys Tyr 325 330 335 ggc ccc tcc cag gac aca ccg gtc acc tca cgt tag gcacccaccg 1354 Gly Pro Ser Gln Asp Thr Pro Val Thr Ser Arg 340 345 gaccctgacc taaagtagag gccatggcag acgacaagca gaaggcggca gcaaaggccg 1414 cgaaggcaga ggcacgggcc gccaagcggg cgaagagcaa ggagacgcgc gcccagctct 1474 ggcaggcgtt caacatccag cgcaagcagg acaagatgct cgtcccgctg atggtgctct 1534 ccgtcgtggg tgtggccgcg ctcctcttcc tcatcggcct ggcctggggc ggccagtggt 1594 tcacgctcat cctgggtatc ttcctgggtg ccgtcctggc gatgtacatc 1644 <210> 48 <211> 347 <212> PRT <213> Corynebacterium humireducens <400> 48 Val Thr Ile Ala Pro Glu Gly Arg Lys Leu Leu Arg Val Glu Lys Arg 1 5 10 15 Asn Ala Gln Thr Pro Ile Glu Thr Lys Pro Arg Trp Ile Arg Asn Ala 20 25 30 Val Lys Thr Gly Pro Glu Tyr Glu Asp Met Lys Lys Lys Val Ala Gly 35 40 45 Ala Ser Leu His Thr Val Cys Gln Glu Ala Gly Cys Pro Asn Ile His 50 55 60 Glu Cys Trp Glu Ser Arg Glu Ala Thr Phe Leu Ile Gly Gly Ala Asn 65 70 75 80 Cys Ser Arg Arg Cys Asp Phe Cys Gln Ile Asn Ser Ala Lys Pro Glu 85 90 95 Pro Leu Asp Arg Asp Glu Pro Arg Arg Val Ala Glu Ser Val Arg Glu 100 105 110 Met Gly Leu Asn Tyr Ser Thr Ile Thr Gly Val Thr Arg Asp Asp Leu 115 120 125 Glu Asp Glu Gly Ala Trp Leu Tyr Ala Glu Val Val Arg Gln Ile His 130 135 140 Glu Leu Asn Pro Asn Thr Gly Val Glu Asn Leu Val Pro Asp Phe Ser 145 150 155 160 Gly Arg Ser Glu Leu Leu Glu Gln Val Phe Glu Ser Arg Pro Glu Val 165 170 175 Phe Ala His Asn Leu Glu Thr Val Pro Arg Ile Phe Lys Arg Ile Arg 180 185 190 Pro Ala Phe Arg Tyr Glu Arg Ser Leu Asp Val Ile Arg Gln Ala His 195 200 205 Asp Tyr Gly Leu Ile Thr Lys Ser Asn Leu Ile Leu Gly Met Gly Glu 210 215 220 Thr Leu Asp Glu Ile Arg Glu Ala Leu Thr Asp Leu His Ser Ala Gly 225 230 235 240 Cys Asp Ile Ile Thr Ile Thr Gln Tyr Leu Arg Pro Gly Pro Met Tyr 245 250 255 His Pro Ile Asp Arg Trp Val Lys Pro Glu Glu Phe Val Glu His Lys 260 265 270 Asp Phe Ala Glu Glu Leu Gly Phe Gly Ala Val Met Ser Gly Pro Leu 275 280 285 Val Arg Ser Ser Tyr Arg Ala Gly Arg Leu Tyr Ser Gln Ala Met Ala 290 295 300 Ala Arg Gly Arg Glu Ile Pro Asp His Leu Lys His Leu Ala Glu Thr 305 310 315 320 Ser Gln Gly Ser Thr Asp Gln Glu Ala Thr Thr Leu Leu Ala Lys Tyr 325 330 335 Gly Pro Ser Gln Asp Thr Pro Val Thr Ser Arg 340 345 <210> 49 <211> 1389 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1089) <223> gene lipB <400> 49 gaaggtcggc atcacctccg tggccaccga acgcctcggc gaggtcgtct acgcggagct 60 cccgcaggtc ggtgacgccg tcaccgccgg tgaaccgtgc ggcgaggtcg agtccacgaa 120 gtcggtctcc gacctctacg cgccggtcac gggcaccgtc accgaggtca acgacgcggt 180 ccacgacgac tacgccgtga tcaacgacga ccccttcggc gcgggctggc tcttcgccgt 240 cgaggtcgac gaggtcggac cgctcatgac cgccgccgag tacgcggagg ccaacggcgt 300 gtg acc gcc cgg gac cgg tac cct ggt ccc atc atg act gca ccc cgt 348 Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg 1 5 10 15 gag ccc ttc ttc cct gcg gac cgt tcc atc cgt gcc tcc ggc gag ccc 396 Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro 20 25 30 ccg gag atc cgt tat ctc ggc gtc gtc gac tac cag gag gcc tgg aat 444 Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn 35 40 45 ctg cag gcc gag ctg gcg tcg caa cgc gcg gcc ggg gag atc ggt gac 492 Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp 50 55 60 cag ctc ctc atc ctc gag cac ccg agc gtg tac acc gcc ggc aag cgc 540 Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg 65 70 75 80 acg cag ccg gag gac cgc ccc acc aac ggc ctg ccg gtc atc gac gtc 588 Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val 85 90 95 gac cgg ggt ggc cgc atc acc tgg cac ggc gag gga cag ctg gtg gtc 636 Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val 100 105 110 tac ccg atc atc aaa ctg gcc gac ccg gtg gac gtc gtg gac tat gtc 684 Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val 115 120 125 cgc cgc ctc gag gag gcg gtg atc gcg acg gtg cgg gag gtg ggc gtc 732 Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val 130 135 140 ggc aat gcg ggt cgc atc gac ggc cgc tcc gga gtc tgg gtc ccc gcc 780 Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala 145 150 155 160 gac ggg acc cga cgc gac cgg aag gtg gcg gcg ctc ggc atc cgt gtc 828 Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val 165 170 175 acc cgc ggg gtg acc atg cac ggg ctg agc ctg aac tgc tcg aac acg 876 Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr 180 185 190 ctc gag cac tac ggg cac atc gtg gcg tgc ggc atc gac gac gcc gac 924 Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp 195 200 205 gtc acc acc ctc agc atg gaa ctg ggg cgc gac gtc agc gtc ggg gag 972 Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu 210 215 220 gtc acg gca ccg ctt gtc gac gcc ctc ctg gcc gcc ctc gcc ggt gaa 1020 Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu 225 230 235 240 ctc gcc gtc gcg gac cac acc ttc gga tcg gcg ccc gat ccg acg aag 1068 Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys 245 250 255 att ccc gcg aaa acg cgc tga cctctaagtt tatccctatc agtagaaaga 1119 Ile Pro Ala Lys Thr Arg 260 gtatggtggc gttgtgacta tcgcacctga aggacgcaag ctgctccggg tggagaagcg 1179 caatgcgcag acccccatcg agacgaagcc ccgttggatc cgcaacgcgg tgaagaccgg 1239 ccccgagtac gaggacatga agaagaaggt ggcgggcgcc tccctgcaca ccgtctgtca 1299 ggaggcgggc tgccccaaca tccacgagtg ctgggagtcc cgcgaggcga cgtttctcat 1359 cggcggcgcc aactgctccc gccgctgcga 1389 <210> 50 <211> 262 <212> PRT <213> Corynebacterium humireducens <400> 50 Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg 1 5 10 15 Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro 20 25 30 Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn 35 40 45 Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp 50 55 60 Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg 65 70 75 80 Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val 85 90 95 Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val 100 105 110 Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val 115 120 125 Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val 130 135 140 Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala 145 150 155 160 Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val 165 170 175 Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr 180 185 190 Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp 195 200 205 Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu 210 215 220 Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu 225 230 235 240 Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys 245 250 255 Ile Pro Ala Lys Thr Arg 260 <210> 51 <211> 2010 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1710) <223> gene lpd <400> 51 caactgacat cacagagaga aggcacacat gtccatcttc gagcagatca tcgtcgccat 60 caacaacttc gtcggcccgg gcatccacgc cctgtcctcc gcttcctcca acgccgcact 120 ggccatcggc ctcttctagg tcgaccccgg aagcatctgc ttatcgacgc cccggccccc 180 ggtcggggcg ttcgtcgttg cggcaccaca gtcccgccac cgtcccaccg cccccacggg 240 ggcgggatcc ttttgtgggg ggcggtgaca cgggggaaac ggtcacctat ccttggtggc 300 gtg act gaa cat tat gac gtt gtt gta ctc ggt gcg ggc ccc ggt ggc 348 Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly 1 5 10 15 tac gtc gcc gcc atc cgc gca gcc cag ctc ggc aag aag gtt gct gtc 396 Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val 20 25 30 gtc gag aag cag tac tgg ggc gga gtc tgc ctc aac gtg ggc tgc atc 444 Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile 35 40 45 ccc tcg aag gcg ctg atc aag aac gcc gag atc gcc cac atc ttc acg 492 Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr 50 55 60 cac gag aag aag acg ttc ggc atc aac ggc gac gtc tcc ttc aac tac 540 His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr 65 70 75 80 gag gac gcc cac aag cgc tcc cgt cag gtg tcg gag aag atc gtc ggc 588 Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly 85 90 95 ggc gtc cac tac ctg atg aag aag aac aag atc acc gag atc gac ggc 636 Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly 100 105 110 ttc gga acc ttc agg gac gcc aag acc atc gag gtc agc gag ggc aag 684 Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys 115 120 125 gac gcc ggc aag gtc gtc acc ttc gac gac tgc atc atc gcc acc ggc 732 Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly 130 135 140 tcc gtg gtc aac agc ctc cgt ggc gtc gag ttc tcc gag aac gtc gtc 780 Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val 145 150 155 160 tcc tac gag gag cag atc ctc aac ccg gtc gcc ccg gag aag atg gtc 828 Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val 165 170 175 atc gtc ggt gcc ggc gcc atc ggc atg gag ttc gcc tac gtg ctc tcc 876 Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser 180 185 190 aac tac ggc gtc gac gtc acc gtc atc gag tac atg gac cgt gtc ctg 924 Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu 195 200 205 ccg aac gag gac gag gac gtc tcc aag gtc atc gcc aag gcc tac aag 972 Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys 210 215 220 aag atg ggc gtc acg ctc ctc ccg ggc cac gcc acc acc gcc gtg cgc 1020 Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg 225 230 235 240 gac aac ggc gac tcc gtc gag gtc gac tac cag aag aag ggc tcg gac 1068 Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp 245 250 255 aag acc gag acc atc gtc gcc gac cgc gtc ctc gtc tcc gtc ggc ttc 1116 Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe 260 265 270 cgc ccg cgt gtc gag ggc ttc ggc ctg gag aac acc ggc gtc gag ctc 1164 Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu 275 280 285 acc gag cgc ggt gcc atc gcc atc gac gac ttc atg cgc acc aac gtc 1212 Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val 290 295 300 gag cac atc tac gcc atc ggt gac gtc acc gcc aag ctg cag ctg gcc 1260 Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala 305 310 315 320 cac gtc gcc gag gcg cag ggc atc gtc gcc gcc gag acc atc gcc ggc 1308 His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly 325 330 335 gcc gag acc cac gag ctg ggc gac tac atg atg atg ccg cgc gcc acc 1356 Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr 340 345 350 ttc tgt aac ccg cag gtc gcg tcc ttc ggc tac acc gag gcg cag gcc 1404 Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala 355 360 365 aag gag aag tgg ccg gac cgc gag atc aag gtc gcg tcc ttc ccg ttc 1452 Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe 370 375 380 tcc gcg aac ggc aag gcc gtc ggc ctg gcc gag acc gag ggc ttc gcc 1500 Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala 385 390 395 400 aag atc gtc gcc gac gcc gag tac ggc gag ctg ctg ggc ggc cac ctg 1548 Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu 405 410 415 gtc ggc gcc aac gcg tcc gag ctg ctc aac gag ctg gtc ctc gcg cag 1596 Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln 420 425 430 aac tac gat ctc acc acc gag gag atc agc cgc gcg gtc cac atc cac 1644 Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His 435 440 445 ccg acc ctg tcg gag gcc gtc aag gag gcc gca cac ggc atc gac ggc 1692 Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly 450 455 460 cac atg atc aac ttc tag tcccccggaa aaaggggcct cccggctggg 1740 His Met Ile Asn Phe 465 aggccccttt tctcatgctt tccgacgccc ccggggcgtc gccaagcgcc tagttgtagg 1800 gcgcgaccga cgaccggtgg gcgtcgatgg tgatctcctc gtggatcggc gggaacgcgc 1860 gctgcgcgca gttctcccgc gggcacacgc ggcagccggc gccgatcggg gtggcgctgg 1920 acaggtcggc gaggttgagt ccctgcgcgt agaccgtgcg gtcggcgtgg cgggcctcgc 1980 agcccaggcc gatggcgaag agcttgccgg 2010 <210> 52 <211> 469 <212> PRT <213> Corynebacterium humireducens <400> 52 Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly 1 5 10 15 Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val 20 25 30 Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile 35 40 45 Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr 50 55 60 His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr 65 70 75 80 Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly 85 90 95 Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly 100 105 110 Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys 115 120 125 Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly 130 135 140 Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val 145 150 155 160 Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val 165 170 175 Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser 180 185 190 Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu 195 200 205 Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys 210 215 220 Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg 225 230 235 240 Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp 245 250 255 Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe 260 265 270 Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu 275 280 285 Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val 290 295 300 Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala 305 310 315 320 His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly 325 330 335 Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr 340 345 350 Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala 355 360 365 Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe 370 375 380 Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala 385 390 395 400 Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu 405 410 415 Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln 420 425 430 Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His 435 440 445 Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly 450 455 460 His Met Ile Asn Phe 465 <210> 53 <211> 1866 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1566) <223> gene lysC <400> 53 gccggtgccc gcccaccaca ggggacggct gatcgcgttc cagaaggggg aacccttgag 60 ggagccgtcg gcgggggcct cctccgcgat gcggtggcgc accacggttc cccacgcgat 120 ggtgagggcg gaggccagtg cgaacagcac ggccagcaga ttgctgtgca cctcatccac 180 cttagtgaac gggggccttt ccgcagggcc gggacccgcc cactcgacta ctcggacgac 240 cccggctccg ggtacgatga agcagaactt tggcacccca gacgaaaggc ggcctacacg 300 gtg gct ctg atc gtt cag aaa tat gga ggc tcc tcc ctg gag acc gcg 348 Val Ala Leu Ile Val Gln Lys Tyr Gly Gly Ser Ser Leu Glu Thr Ala 1 5 10 15 gag cgg atc cgc gct gtc gcc gaa cgg atc gtc gcc acg aaa aag gcc 396 Glu Arg Ile Arg Ala Val Ala Glu Arg Ile Val Ala Thr Lys Lys Ala 20 25 30 ggc aac gac gtc gtc gtg gtc tgc tcc gcg atg ggc gac acc acg gac 444 Gly Asn Asp Val Val Val Val Cys Ser Ala Met Gly Asp Thr Thr Asp 35 40 45 gaa ctc ctg gac ctc gcc gcc cag gtg aac ccg gtt ccg ccg gcc cgt 492 Glu Leu Leu Asp Leu Ala Ala Gln Val Asn Pro Val Pro Pro Ala Arg 50 55 60 gag atg gac atg ctg ctc acc gcg ggt gag cgc atc tcc aac gcg ctg 540 Glu Met Asp Met Leu Leu Thr Ala Gly Glu Arg Ile Ser Asn Ala Leu 65 70 75 80 gtc gcg atg gcg atc tcc tcg ctc ggc gcg aag gcg cag tcc ttc acc 588 Val Ala Met Ala Ile Ser Ser Leu Gly Ala Lys Ala Gln Ser Phe Thr 85 90 95 ggc tcg cag gcc ggt gtg ctc acc acc gag cgt cac ggc aac gcc cgc 636 Gly Ser Gln Ala Gly Val Leu Thr Thr Glu Arg His Gly Asn Ala Arg 100 105 110 atc gtc gac gtc acc ccg gac cgc gtc cgc gag gcg ctg gac gag ggc 684 Ile Val Asp Val Thr Pro Asp Arg Val Arg Glu Ala Leu Asp Glu Gly 115 120 125 aac atc tgc atc gtc gcc ggt ttc cag ggc atc aac aag gac acc cgc 732 Asn Ile Cys Ile Val Ala Gly Phe Gln Gly Ile Asn Lys Asp Thr Arg 130 135 140 gac gtc acc acc ctg ggt cgt ggc ggt tcc gac acc aca gcg gtg gcc 780 Asp Val Thr Thr Leu Gly Arg Gly Gly Ser Asp Thr Thr Ala Val Ala 145 150 155 160 ctg gcg gcg gcg ctc aac gcc gac gtg tgc gag atc tac tcc gac gtc 828 Leu Ala Ala Ala Leu Asn Ala Asp Val Cys Glu Ile Tyr Ser Asp Val 165 170 175 gac ggc gtc tac acc gcc gat ccg cgc atc gtc ccg aac gcg cag aag 876 Asp Gly Val Tyr Thr Ala Asp Pro Arg Ile Val Pro Asn Ala Gln Lys 180 185 190 ctc gag aag ctc tcc ttc gag gag atg ctc gag ctg gcg gcc gtc ggt 924 Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu Leu Ala Ala Val Gly 195 200 205 tcg aag atc ctg gtg ctg cgt gcc gtc gag tac gca cgt gcc ttc aat 972 Ser Lys Ile Leu Val Leu Arg Ala Val Glu Tyr Ala Arg Ala Phe Asn 210 215 220 gtt ccc ctg cga gtt cgc tcg tct tac agc aat gac acc ggc acc ctg 1020 Val Pro Leu Arg Val Arg Ser Ser Tyr Ser Asn Asp Thr Gly Thr Leu 225 230 235 240 atc gcc ggt tcg atg gag gat atc ccc gtg gaa gaa gca gtt ctc act 1068 Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr 245 250 255 ggc gtt gcc acc gac aag tcc gag gcc aag gtc acc atc ctg ggt att 1116 Gly Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Ile Leu Gly Ile 260 265 270 ccc gac aag ccg ggc gag gcg gcc acc gtc ttc cgc gcc gtc gcg gac 1164 Pro Asp Lys Pro Gly Glu Ala Ala Thr Val Phe Arg Ala Val Ala Asp 275 280 285 gcg gag atc aac atc gac atg gtc ctg cag aac gtc tcc tcc ctg gag 1212 Ala Glu Ile Asn Ile Asp Met Val Leu Gln Asn Val Ser Ser Leu Glu 290 295 300 gac ggc acg acc gac atc acc ttc acc tgc ccg cgt gcc gac ggc ccc 1260 Asp Gly Thr Thr Asp Ile Thr Phe Thr Cys Pro Arg Ala Asp Gly Pro 305 310 315 320 cgc gcc atc gag ctg ctc aag aag atg cag gcg aag agc ggc tgg tcc 1308 Arg Ala Ile Glu Leu Leu Lys Lys Met Gln Ala Lys Ser Gly Trp Ser 325 330 335 aac gtg ctc tac gac gac cag gtg ggc aag gtc tcc ctc gtg ggt gcc 1356 Asn Val Leu Tyr Asp Asp Gln Val Gly Lys Val Ser Leu Val Gly Ala 340 345 350 ggc atg aag tcc cac ccg ggc gtc acc gcc gac ttc gcc gag gcg ctc 1404 Gly Met Lys Ser His Pro Gly Val Thr Ala Asp Phe Ala Glu Ala Leu 355 360 365 cgc gac gcc ggc gtg aac atc gag cta atc tcc acc tcg gag atc cgc 1452 Arg Asp Ala Gly Val Asn Ile Glu Leu Ile Ser Thr Ser Glu Ile Arg 370 375 380 atc tcg gtg ctc acc cgc gag gcc gac ctg gac gcc gcc gcc cgt gcc 1500 Ile Ser Val Leu Thr Arg Glu Ala Asp Leu Asp Ala Ala Ala Arg Ala 385 390 395 400 ctg cac gag aag ttc gag ctc ggc ggc gac gcc gag gcc acc gtc tac 1548 Leu His Glu Lys Phe Glu Leu Gly Gly Asp Ala Glu Ala Thr Val Tyr 405 410 415 gcc ggc acc ggc cgc tag acaatccctt ccacagagga gctttcactc 1596 Ala Gly Thr Gly Arg 420 tcatgaccac cattgctgtc gtcggtgcca ccggccaggt cggccgcgtg atgcgctcga 1656 tcctggagga gcgcaacttc ccggccgaca aggtccgttt cttcgcgtcc ccgcgctccg 1716 cgggcaccgt catcgagttc cgcggtgagg acatcaccgt cgaggacctc acccagatca 1776 ccccggagtc cgtcaaggac gtcgacatcg ccctcttctc cgccggtggt tccacctccc 1836 gtgagtgggc cccggtcttc gccgaggccg 1866 <210> 54 <211> 421 <212> PRT <213> Corynebacterium humireducens <400> 54 Val Ala Leu Ile Val Gln Lys Tyr Gly Gly Ser Ser Leu Glu Thr Ala 1 5 10 15 Glu Arg Ile Arg Ala Val Ala Glu Arg Ile Val Ala Thr Lys Lys Ala 20 25 30 Gly Asn Asp Val Val Val Val Cys Ser Ala Met Gly Asp Thr Thr Asp 35 40 45 Glu Leu Leu Asp Leu Ala Ala Gln Val Asn Pro Val Pro Pro Ala Arg 50 55 60 Glu Met Asp Met Leu Leu Thr Ala Gly Glu Arg Ile Ser Asn Ala Leu 65 70 75 80 Val Ala Met Ala Ile Ser Ser Leu Gly Ala Lys Ala Gln Ser Phe Thr 85 90 95 Gly Ser Gln Ala Gly Val Leu Thr Thr Glu Arg His Gly Asn Ala Arg 100 105 110 Ile Val Asp Val Thr Pro Asp Arg Val Arg Glu Ala Leu Asp Glu Gly 115 120 125 Asn Ile Cys Ile Val Ala Gly Phe Gln Gly Ile Asn Lys Asp Thr Arg 130 135 140 Asp Val Thr Thr Leu Gly Arg Gly Gly Ser Asp Thr Thr Ala Val Ala 145 150 155 160 Leu Ala Ala Ala Leu Asn Ala Asp Val Cys Glu Ile Tyr Ser Asp Val 165 170 175 Asp Gly Val Tyr Thr Ala Asp Pro Arg Ile Val Pro Asn Ala Gln Lys 180 185 190 Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu Leu Ala Ala Val Gly 195 200 205 Ser Lys Ile Leu Val Leu Arg Ala Val Glu Tyr Ala Arg Ala Phe Asn 210 215 220 Val Pro Leu Arg Val Arg Ser Ser Tyr Ser Asn Asp Thr Gly Thr Leu 225 230 235 240 Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr 245 250 255 Gly Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Ile Leu Gly Ile 260 265 270 Pro Asp Lys Pro Gly Glu Ala Ala Thr Val Phe Arg Ala Val Ala Asp 275 280 285 Ala Glu Ile Asn Ile Asp Met Val Leu Gln Asn Val Ser Ser Leu Glu 290 295 300 Asp Gly Thr Thr Asp Ile Thr Phe Thr Cys Pro Arg Ala Asp Gly Pro 305 310 315 320 Arg Ala Ile Glu Leu Leu Lys Lys Met Gln Ala Lys Ser Gly Trp Ser 325 330 335 Asn Val Leu Tyr Asp Asp Gln Val Gly Lys Val Ser Leu Val Gly Ala 340 345 350 Gly Met Lys Ser His Pro Gly Val Thr Ala Asp Phe Ala Glu Ala Leu 355 360 365 Arg Asp Ala Gly Val Asn Ile Glu Leu Ile Ser Thr Ser Glu Ile Arg 370 375 380 Ile Ser Val Leu Thr Arg Glu Ala Asp Leu Asp Ala Ala Ala Arg Ala 385 390 395 400 Leu His Glu Lys Phe Glu Leu Gly Gly Asp Ala Glu Ala Thr Val Tyr 405 410 415 Ala Gly Thr Gly Arg 420 <210> 55 <211> 1794 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1494) <223> gene metB <400> 55 acctccccgg acgggagaga ctgctcggtg tgatcgaccg gctccgcgcg gcgcagcccg 60 gcctgacgac ggtgctggtg acgcatcacg tggaggagat cgcggcgtcg acaagcgatg 120 tcctgctgct caaggagggc cgcgtcctgg cctccggacc cgtcgacgag acgctgacct 180 cggggaacct gtcggcactc tacgacctgc cggtgtcgtt ggagaaggtc cacggacgct 240 ggttcgcgct ctgcatataa ttcccggatg ctgccgggaa agtgaaccgc ttggtctata 300 gtg gcc gac atg acc cac gga ctt tct ggt ttc tcg tct cgt tcc atc 348 Val Ala Asp Met Thr His Gly Leu Ser Gly Phe Ser Ser Arg Ser Ile 1 5 10 15 cat gcc gga tac gag cct gac agc ctc tac ggc ccg atc aac acg ccg 396 His Ala Gly Tyr Glu Pro Asp Ser Leu Tyr Gly Pro Ile Asn Thr Pro 20 25 30 atc tac gcg tcg acc acc ttc gcg cag gac ggc ctc aac gag ctg cgc 444 Ile Tyr Ala Ser Thr Thr Phe Ala Gln Asp Gly Leu Asn Glu Leu Arg 35 40 45 ggt ggc ttc gag tac acc cgc tgc ggc aac ccc acg atc gac gcc ctc 492 Gly Gly Phe Glu Tyr Thr Arg Cys Gly Asn Pro Thr Ile Asp Ala Leu 50 55 60 gaa aag acc gtc gca gcc ctg gaa ggc gca cag tac ggc cgc gcg ttc 540 Glu Lys Thr Val Ala Ala Leu Glu Gly Ala Gln Tyr Gly Arg Ala Phe 65 70 75 80 tcc tcc ggc atg gcc gcc acc gac gtc atc ctg cgt atc ctc ctg cgc 588 Ser Ser Gly Met Ala Ala Thr Asp Val Ile Leu Arg Ile Leu Leu Arg 85 90 95 ccc ggt gac cac ctc atc ctc ggc cac gac gcc tac ggc ggc acc tgg 636 Pro Gly Asp His Leu Ile Leu Gly His Asp Ala Tyr Gly Gly Thr Trp 100 105 110 cgc ctc atc gac cag gcc ttc ggc cag tgg ggc gtc gag tac acc gtc 684 Arg Leu Ile Asp Gln Ala Phe Gly Gln Trp Gly Val Glu Tyr Thr Val 115 120 125 gtc gac acc acc gac gtt gcc gcc gtc gag gcc gcc ctc cgc ccg aac 732 Val Asp Thr Thr Asp Val Ala Ala Val Glu Ala Ala Leu Arg Pro Asn 130 135 140 acc aag ctc atc tgg ctg gag acc ccg acc aac ccg gcc ctg tcc atc 780 Thr Lys Leu Ile Trp Leu Glu Thr Pro Thr Asn Pro Ala Leu Ser Ile 145 150 155 160 acc gac atc gcc gcc atc gcc gcg atc aag cag cag gcc gcc ctc gtc 828 Thr Asp Ile Ala Ala Ile Ala Ala Ile Lys Gln Gln Ala Ala Leu Val 165 170 175 gtg gac aac acc ttc gcg tcc ccg tac ctg cag cag ccg ctc gcc ctc 876 Val Asp Asn Thr Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Ala Leu 180 185 190 ggc gcg gac cac gtc ctg cac tcc gcc acc aag tac ctc ggc ggt cac 924 Gly Ala Asp His Val Leu His Ser Ala Thr Lys Tyr Leu Gly Gly His 195 200 205 tcc gac gtc gtc ggc ggc ctc gtg gtg acc aac tgc gcc gag ttc gac 972 Ser Asp Val Val Gly Gly Leu Val Val Thr Asn Cys Ala Glu Phe Asp 210 215 220 gag cag ctc ctc tgg ttc cag ggc ggc gtc ggc ccc atc ccg tcc ccc 1020 Glu Gln Leu Leu Trp Phe Gln Gly Gly Val Gly Pro Ile Pro Ser Pro 225 230 235 240 ttc gac gcc tac ctc acc gcc cgc ggc atc aag acc ctg gcc gtg cgc 1068 Phe Asp Ala Tyr Leu Thr Ala Arg Gly Ile Lys Thr Leu Ala Val Arg 245 250 255 atg gag cgc cac tgc gac aac gcc gag gcc atc gcc gcg cac ctc gag 1116 Met Glu Arg His Cys Asp Asn Ala Glu Ala Ile Ala Ala His Leu Glu 260 265 270 gcc tcc gac cag gtc gcg acc gtg ctc tac ccc ggc ctc gag tcc cac 1164 Ala Ser Asp Gln Val Ala Thr Val Leu Tyr Pro Gly Leu Glu Ser His 275 280 285 ccg ggc cac gag gtg gcc aag cgc cag atg aag cgc ttc ggc ggc atg 1212 Pro Gly His Glu Val Ala Lys Arg Gln Met Lys Arg Phe Gly Gly Met 290 295 300 atc tcc gtg cgc ttc cgc tcc gag gag gcc gcc cgc gtc ttc tgc ctg 1260 Ile Ser Val Arg Phe Arg Ser Glu Glu Ala Ala Arg Val Phe Cys Leu 305 310 315 320 aac acc cga ctg atc tgt ctg gcc gag tct ctc ggc ggc gtc gag tcg 1308 Asn Thr Arg Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val Glu Ser 325 330 335 ctc gtc gag cac ccg gcc acc atg acc cac cag tcc gcc acc ggc tcc 1356 Leu Val Glu His Pro Ala Thr Met Thr His Gln Ser Ala Thr Gly Ser 340 345 350 cag ctg gag gtc ccg cgt gac ctg gtg cgc atc tcc atc ggc atc gag 1404 Gln Leu Glu Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly Ile Glu 355 360 365 gac gtc gag gac ctc atc gcg gac atc gac gtc gcc ctc cag gcc gtc 1452 Asp Val Glu Asp Leu Ile Ala Asp Ile Asp Val Ala Leu Gln Ala Val 370 375 380 gcg gcc gcg gag gcg gcc gct gag gcc tcc gtc gac gcc tga 1494 Ala Ala Ala Glu Ala Ala Ala Glu Ala Ser Val Asp Ala 385 390 395 gtgatgaccg ccccgcacac ggtcgagagg acatgtgcgg gacggctcct cccgggcggc 1554 gcagtgtcga gacgggtact cctcccgaac atgtgagccc gcccctggct ccggccccct 1614 cagggcagcc ggatcgccgc cacaccgacc accatgagcg ccaggcccac cgcctggacc 1674 agggtggtcg gtttcctctt cgcccccagc agcccgaagt ggtcgatgag gaggctgccg 1734 accatcatgc cgagcaggat cgtcatgacg gtcagacccg tgccgatgac gggggagagg 1794 <210> 56 <211> 397 <212> PRT <213> Corynebacterium humireducens <400> 56 Val Ala Asp Met Thr His Gly Leu Ser Gly Phe Ser Ser Arg Ser Ile 1 5 10 15 His Ala Gly Tyr Glu Pro Asp Ser Leu Tyr Gly Pro Ile Asn Thr Pro 20 25 30 Ile Tyr Ala Ser Thr Thr Phe Ala Gln Asp Gly Leu Asn Glu Leu Arg 35 40 45 Gly Gly Phe Glu Tyr Thr Arg Cys Gly Asn Pro Thr Ile Asp Ala Leu 50 55 60 Glu Lys Thr Val Ala Ala Leu Glu Gly Ala Gln Tyr Gly Arg Ala Phe 65 70 75 80 Ser Ser Gly Met Ala Ala Thr Asp Val Ile Leu Arg Ile Leu Leu Arg 85 90 95 Pro Gly Asp His Leu Ile Leu Gly His Asp Ala Tyr Gly Gly Thr Trp 100 105 110 Arg Leu Ile Asp Gln Ala Phe Gly Gln Trp Gly Val Glu Tyr Thr Val 115 120 125 Val Asp Thr Thr Asp Val Ala Ala Val Glu Ala Ala Leu Arg Pro Asn 130 135 140 Thr Lys Leu Ile Trp Leu Glu Thr Pro Thr Asn Pro Ala Leu Ser Ile 145 150 155 160 Thr Asp Ile Ala Ala Ile Ala Ala Ile Lys Gln Gln Ala Ala Leu Val 165 170 175 Val Asp Asn Thr Phe Ala Ser Pro Tyr Leu Gln Gln Pro Leu Ala Leu 180 185 190 Gly Ala Asp His Val Leu His Ser Ala Thr Lys Tyr Leu Gly Gly His 195 200 205 Ser Asp Val Val Gly Gly Leu Val Val Thr Asn Cys Ala Glu Phe Asp 210 215 220 Glu Gln Leu Leu Trp Phe Gln Gly Gly Val Gly Pro Ile Pro Ser Pro 225 230 235 240 Phe Asp Ala Tyr Leu Thr Ala Arg Gly Ile Lys Thr Leu Ala Val Arg 245 250 255 Met Glu Arg His Cys Asp Asn Ala Glu Ala Ile Ala Ala His Leu Glu 260 265 270 Ala Ser Asp Gln Val Ala Thr Val Leu Tyr Pro Gly Leu Glu Ser His 275 280 285 Pro Gly His Glu Val Ala Lys Arg Gln Met Lys Arg Phe Gly Gly Met 290 295 300 Ile Ser Val Arg Phe Arg Ser Glu Glu Ala Ala Arg Val Phe Cys Leu 305 310 315 320 Asn Thr Arg Leu Ile Cys Leu Ala Glu Ser Leu Gly Gly Val Glu Ser 325 330 335 Leu Val Glu His Pro Ala Thr Met Thr His Gln Ser Ala Thr Gly Ser 340 345 350 Gln Leu Glu Val Pro Arg Asp Leu Val Arg Ile Ser Ile Gly Ile Glu 355 360 365 Asp Val Glu Asp Leu Ile Ala Asp Ile Asp Val Ala Leu Gln Ala Val 370 375 380 Ala Ala Ala Glu Ala Ala Ala Glu Ala Ser Val Asp Ala 385 390 395 <210> 57 <211> 1581 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1281) <223> gene metF <400> 57 ggttcacggt gtgctccttg ttcagtgggt cattctccct cccagactag cggcgaagcc 60 tcgatcaggg cgggtttccg gatgtgtgga acctggggaa atgaaactga accgcttggt 120 ccatttttgt ccgaaaatcg gccctcgggg gtgaattata ttcatgctag ggtggggtta 180 tcgccgcagg tcacgcggca aaccggaaat agcaccttta ggaaaattac ccgccgcaaa 240 ccgttggctt gctacattca cagccacttg cccaacggga tgtctgaagg aggaaaacac 300 atg aac gca aac gcg ccg cag tac agc gcc aag cgt cga ttc tcc gcc 348 Met Asn Ala Asn Ala Pro Gln Tyr Ser Ala Lys Arg Arg Phe Ser Ala 1 5 10 15 tcc gcc ccg ttg gac acc ttc gat ccc gag gaa tgg cgc cag gcc ccc 396 Ser Ala Pro Leu Asp Thr Phe Asp Pro Glu Glu Trp Arg Gln Ala Pro 20 25 30 gtg ccg gag cgc acc tcc ctc tcc ttc gag gtc atg ccc ccg cgc cac 444 Val Pro Glu Arg Thr Ser Leu Ser Phe Glu Val Met Pro Pro Arg His 35 40 45 gac gcg gac gag gcc aag atc gac gaa ctg ctg gac acg ctg cag tcc 492 Asp Ala Asp Glu Ala Lys Ile Asp Glu Leu Leu Asp Thr Leu Gln Ser 50 55 60 tac aac ccg gac tac atc tcc gtc acc agc tcc cgg aac tcc ggc tgg 540 Tyr Asn Pro Asp Tyr Ile Ser Val Thr Ser Ser Arg Asn Ser Gly Trp 65 70 75 80 ctc gag ggc acc gcg aag ttc atc gcg aag atc agc gcc cag acc cgc 588 Leu Glu Gly Thr Ala Lys Phe Ile Ala Lys Ile Ser Ala Gln Thr Arg 85 90 95 atc ccg acg gtc gcc cac ctg gcc tgc acc gcc ggc acc cgc gag gag 636 Ile Pro Thr Val Ala His Leu Ala Cys Thr Ala Gly Thr Arg Glu Glu 100 105 110 ctc atc ggc tgg atc aac cgg ctc atg gac tcc ggc gtc cgc ggc ctg 684 Leu Ile Gly Trp Ile Asn Arg Leu Met Asp Ser Gly Val Arg Gly Leu 115 120 125 ctc gcc ctc cgc ggc gac ctc gca gaa gga cag acc ggt atg cct gag 732 Leu Ala Leu Arg Gly Asp Leu Ala Glu Gly Gln Thr Gly Met Pro Glu 130 135 140 ggt tac ctc aag cac gcg acc gac ctc atc aac ctc atc cag gag atc 780 Gly Tyr Leu Lys His Ala Thr Asp Leu Ile Asn Leu Ile Gln Glu Ile 145 150 155 160 gag gag gag cag gtc gcc cgc ttc ggc gcc ggc cgc ctc gcc atc ggc 828 Glu Glu Glu Gln Val Ala Arg Phe Gly Ala Gly Arg Leu Ala Ile Gly 165 170 175 gtc gcc gcc tac ccc agc ggc cac gag gag tcg acc agc cgc gac gag 876 Val Ala Ala Tyr Pro Ser Gly His Glu Glu Ser Thr Ser Arg Asp Glu 180 185 190 gac atc gac gtc ctc ctg gcc aag cag cgc ctg ggc gcc gac ttc gcc 924 Asp Ile Asp Val Leu Leu Ala Lys Gln Arg Leu Gly Ala Asp Phe Ala 195 200 205 atc acc cag ctc ttc ttc gac gcc gag gac tac ctc cgc ttc ctg gag 972 Ile Thr Gln Leu Phe Phe Asp Ala Glu Asp Tyr Leu Arg Phe Leu Glu 210 215 220 cgc gcc cgc ctg gca ggc gtc cgc atc ccg ctc atc ccc ggc atc atg 1020 Arg Ala Arg Leu Ala Gly Val Arg Ile Pro Leu Ile Pro Gly Ile Met 225 230 235 240 ccg atg acg agc gtc gcg aga cta cgt cga atg gga cag ctc tcc ggc 1068 Pro Met Thr Ser Val Ala Arg Leu Arg Arg Met Gly Gln Leu Ser Gly 245 250 255 ctt acc gtc ccc gac cgg ctg atc agc cga ctg gag gcg gca ggc ccg 1116 Leu Thr Val Pro Asp Arg Leu Ile Ser Arg Leu Glu Ala Ala Gly Pro 260 265 270 gag agc gag tac cag acg ggc ctc gac ctc acg gcc gag ctc gcg cag 1164 Glu Ser Glu Tyr Gln Thr Gly Leu Asp Leu Thr Ala Glu Leu Ala Gln 275 280 285 acc atc ctg gac gcc ggt gcc ggg ggg atg cac gtg tac acg ttc aac 1212 Thr Ile Leu Asp Ala Gly Ala Gly Gly Met His Val Tyr Thr Phe Asn 290 295 300 cgg ccg gac acc acc acc gaa ctg ctg gac cgc atc ggc atc acc ccc 1260 Arg Pro Asp Thr Thr Thr Glu Leu Leu Asp Arg Ile Gly Ile Thr Pro 305 310 315 320 gac cct cgg gtt agg gag tga ggcgggagac cgccgccgct gcatctcatg 1311 Asp Pro Arg Val Arg Glu 325 ttgatagcga cctgctgcac gcaccgggag cccccgaagc agaccaggtt gtacacccaa 1371 gattttccga aaggcctccc ttttcatgtc ttccaccgct ttccccacct tcaccatcga 1431 gggctacccg cgcgtcggcg ccaaccgcga gctcaagaag gccctcgagt ccttctgggc 1491 cggccgcatc gacgaggaga ccttcatctc ctccgcgcac tccatccgcc tggagaacta 1551 cgcccgtctg cgtgacctcg gcctggacca 1581 <210> 58 <211> 326 <212> PRT <213> Corynebacterium humireducens <400> 58 Met Asn Ala Asn Ala Pro Gln Tyr Ser Ala Lys Arg Arg Phe Ser Ala 1 5 10 15 Ser Ala Pro Leu Asp Thr Phe Asp Pro Glu Glu Trp Arg Gln Ala Pro 20 25 30 Val Pro Glu Arg Thr Ser Leu Ser Phe Glu Val Met Pro Pro Arg His 35 40 45 Asp Ala Asp Glu Ala Lys Ile Asp Glu Leu Leu Asp Thr Leu Gln Ser 50 55 60 Tyr Asn Pro Asp Tyr Ile Ser Val Thr Ser Ser Arg Asn Ser Gly Trp 65 70 75 80 Leu Glu Gly Thr Ala Lys Phe Ile Ala Lys Ile Ser Ala Gln Thr Arg 85 90 95 Ile Pro Thr Val Ala His Leu Ala Cys Thr Ala Gly Thr Arg Glu Glu 100 105 110 Leu Ile Gly Trp Ile Asn Arg Leu Met Asp Ser Gly Val Arg Gly Leu 115 120 125 Leu Ala Leu Arg Gly Asp Leu Ala Glu Gly Gln Thr Gly Met Pro Glu 130 135 140 Gly Tyr Leu Lys His Ala Thr Asp Leu Ile Asn Leu Ile Gln Glu Ile 145 150 155 160 Glu Glu Glu Gln Val Ala Arg Phe Gly Ala Gly Arg Leu Ala Ile Gly 165 170 175 Val Ala Ala Tyr Pro Ser Gly His Glu Glu Ser Thr Ser Arg Asp Glu 180 185 190 Asp Ile Asp Val Leu Leu Ala Lys Gln Arg Leu Gly Ala Asp Phe Ala 195 200 205 Ile Thr Gln Leu Phe Phe Asp Ala Glu Asp Tyr Leu Arg Phe Leu Glu 210 215 220 Arg Ala Arg Leu Ala Gly Val Arg Ile Pro Leu Ile Pro Gly Ile Met 225 230 235 240 Pro Met Thr Ser Val Ala Arg Leu Arg Arg Met Gly Gln Leu Ser Gly 245 250 255 Leu Thr Val Pro Asp Arg Leu Ile Ser Arg Leu Glu Ala Ala Gly Pro 260 265 270 Glu Ser Glu Tyr Gln Thr Gly Leu Asp Leu Thr Ala Glu Leu Ala Gln 275 280 285 Thr Ile Leu Asp Ala Gly Ala Gly Gly Met His Val Tyr Thr Phe Asn 290 295 300 Arg Pro Asp Thr Thr Thr Glu Leu Leu Asp Arg Ile Gly Ile Thr Pro 305 310 315 320 Asp Pro Arg Val Arg Glu 325 <210> 59 <211> 1695 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1395) <223> gene metX <400> 59 tccgcctgtc ggtgggcatc gagaagatca ccgacatcat cgccgacctg gagaacgccc 60 tgaacgccgt ctaagagaca gcttggtcca catacattca cgtcccccac ctggggagac 120 gtggatgtcg gcgacccttc tgtttgcgtg gtcacgcgcg gaaacctacc ctttcaggag 180 accgtgccga atgtaggcac cgaggacgac aaactgtccc ccgggacccc caccagacca 240 ctaggacggt gagtttccat ctctaccctc aacctggccc cccagggcga actcgcgcag 300 gtg cgc atc ggt gat ctc cac acg gag gcc ggt ggc gtc atc aac gac 348 Val Arg Ile Gly Asp Leu His Thr Glu Ala Gly Gly Val Ile Asn Asp 1 5 10 15 gtc acc atc gcc tac cgc cgc tgg ggc gag ttc cgg gga gac ccg gac 396 Val Thr Ile Ala Tyr Arg Arg Trp Gly Glu Phe Arg Gly Asp Pro Asp 20 25 30 ggg atg aac aac ctc gtg ctc atc gaa cac gcc ctc acc ggc gac acc 444 Gly Met Asn Asn Leu Val Leu Ile Glu His Ala Leu Thr Gly Asp Thr 35 40 45 aac gtc gcc gac tgg tgg tgt gac ctg ctc ggc ccc ggc cgg gcg ctc 492 Asn Val Ala Asp Trp Trp Cys Asp Leu Leu Gly Pro Gly Arg Ala Leu 50 55 60 gac acc gac gcg tgg tgc atc gtg tgc acc aac gtc atc ggc ggc tgc 540 Asp Thr Asp Ala Trp Cys Ile Val Cys Thr Asn Val Ile Gly Gly Cys 65 70 75 80 cag ggc tcg acc ggc ccc gcc tcc gaa cac ccc gac ggc ggc cgc tgg 588 Gln Gly Ser Thr Gly Pro Ala Ser Glu His Pro Asp Gly Gly Arg Trp 85 90 95 gga tcc cgc ttc ccg gcg atc tcc atc cgc gac cag gtc acc gcg gag 636 Gly Ser Arg Phe Pro Ala Ile Ser Ile Arg Asp Gln Val Thr Ala Glu 100 105 110 aag cag ttc ctc gac gcc ctc ggc gtc tcc cgc atc cac gcc gtc ctc 684 Lys Gln Phe Leu Asp Ala Leu Gly Val Ser Arg Ile His Ala Val Leu 115 120 125 ggc ggt tcc atg ggc ggt gcc cgc acc ctc gag tgg gcg atg atg tac 732 Gly Gly Ser Met Gly Gly Ala Arg Thr Leu Glu Trp Ala Met Met Tyr 130 135 140 ccg gac aag gtc aac gcc gtg tgc gtc atc gcc gtc tcc gcc cgg gcc 780 Pro Asp Lys Val Asn Ala Val Cys Val Ile Ala Val Ser Ala Arg Ala 145 150 155 160 agc gcc tgg cag atc ggc atc cag tcc gcg cag atc tcc gcc atc gag 828 Ser Ala Trp Gln Ile Gly Ile Gln Ser Ala Gln Ile Ser Ala Ile Glu 165 170 175 cgt gac ccc gcc tgg cag ggc ggc gac ttc tac gac acc ggt gcc tcc 876 Arg Asp Pro Ala Trp Gln Gly Gly Asp Phe Tyr Asp Thr Gly Ala Ser 180 185 190 ccc gac gcc ggc ctc gcc gcc gcc cgc cgc atc gcc cac ctc acc tac 924 Pro Asp Ala Gly Leu Ala Ala Ala Arg Arg Ile Ala His Leu Thr Tyr 195 200 205 cgc ggt gag cag gag ctc gac gag cgt ttc ggc gcc gcc cgc cag gac 972 Arg Gly Glu Gln Glu Leu Asp Glu Arg Phe Gly Ala Ala Arg Gln Asp 210 215 220 ggg gag aac ccg ctg ggc acc ttc cgg gac ccc gcc cag cgt ttc gcc 1020 Gly Glu Asn Pro Leu Gly Thr Phe Arg Asp Pro Ala Gln Arg Phe Ala 225 230 235 240 gtc gag agc tac ctg cag tac cac ggc cgc aag ctg gtc gag cgt ttc 1068 Val Glu Ser Tyr Leu Gln Tyr His Gly Arg Lys Leu Val Glu Arg Phe 245 250 255 gac gcc ggc agc tac gtc acc ctc acc gag gcc ctc aac cgg cac aac 1116 Asp Ala Gly Ser Tyr Val Thr Leu Thr Glu Ala Leu Asn Arg His Asn 260 265 270 atc ggc cgg ggc cgc ggg ggc ctg aac aag gcg ctg gcg gcc tgc acg 1164 Ile Gly Arg Gly Arg Gly Gly Leu Asn Lys Ala Leu Ala Ala Cys Thr 275 280 285 gtg ccg acg atg atc gtc ggc gtc gac acg gac atc ctg tac ccg tac 1212 Val Pro Thr Met Ile Val Gly Val Asp Thr Asp Ile Leu Tyr Pro Tyr 290 295 300 cac cag cag gag cac ctc tcc cgc aac gtc ggt gag ctg atg ggc atc 1260 His Gln Gln Glu His Leu Ser Arg Asn Val Gly Glu Leu Met Gly Ile 305 310 315 320 gcc cgg atc tcc tcc cct gtc ggc cac gac gcc ttc ctg gcg gag ttc 1308 Ala Arg Ile Ser Ser Pro Val Gly His Asp Ala Phe Leu Ala Glu Phe 325 330 335 cgg cag atg gac cgc atc ctc cgg cgc ttc ttc gtc ctc agc gtg ctg 1356 Arg Gln Met Asp Arg Ile Leu Arg Arg Phe Phe Val Leu Ser Val Leu 340 345 350 gac gag gac gcc cag gag gac gac ggc tac ctc atc tag caacccgaac 1405 Asp Glu Asp Ala Gln Glu Asp Asp Gly Tyr Leu Ile 355 360 gtgccgcgag ggggctccca gccatcggtt gggagccccc tcgcggcacg tttcgcgggg 1465 tcacgagccc agcgcgtcca ccgaggccgc caggaacgcc atcgtccccc cgaggacggt 1525 gcagaaggcg gcgtcgaaac gccgggagcg cacggcgaac accccgacga tgcgggagtc 1585 gcacgcgtag cgcaccacgg ccagccacat gagtgagacg ccgagcacga acgtcgcgcg 1645 gcgccagtgc tcggtgagcg cgaagacgct cgccacgacc acgccgagca 1695 <210> 60 <211> 364 <212> PRT <213> Corynebacterium humireducens <400> 60 Val Arg Ile Gly Asp Leu His Thr Glu Ala Gly Gly Val Ile Asn Asp 1 5 10 15 Val Thr Ile Ala Tyr Arg Arg Trp Gly Glu Phe Arg Gly Asp Pro Asp 20 25 30 Gly Met Asn Asn Leu Val Leu Ile Glu His Ala Leu Thr Gly Asp Thr 35 40 45 Asn Val Ala Asp Trp Trp Cys Asp Leu Leu Gly Pro Gly Arg Ala Leu 50 55 60 Asp Thr Asp Ala Trp Cys Ile Val Cys Thr Asn Val Ile Gly Gly Cys 65 70 75 80 Gln Gly Ser Thr Gly Pro Ala Ser Glu His Pro Asp Gly Gly Arg Trp 85 90 95 Gly Ser Arg Phe Pro Ala Ile Ser Ile Arg Asp Gln Val Thr Ala Glu 100 105 110 Lys Gln Phe Leu Asp Ala Leu Gly Val Ser Arg Ile His Ala Val Leu 115 120 125 Gly Gly Ser Met Gly Gly Ala Arg Thr Leu Glu Trp Ala Met Met Tyr 130 135 140 Pro Asp Lys Val Asn Ala Val Cys Val Ile Ala Val Ser Ala Arg Ala 145 150 155 160 Ser Ala Trp Gln Ile Gly Ile Gln Ser Ala Gln Ile Ser Ala Ile Glu 165 170 175 Arg Asp Pro Ala Trp Gln Gly Gly Asp Phe Tyr Asp Thr Gly Ala Ser 180 185 190 Pro Asp Ala Gly Leu Ala Ala Ala Arg Arg Ile Ala His Leu Thr Tyr 195 200 205 Arg Gly Glu Gln Glu Leu Asp Glu Arg Phe Gly Ala Ala Arg Gln Asp 210 215 220 Gly Glu Asn Pro Leu Gly Thr Phe Arg Asp Pro Ala Gln Arg Phe Ala 225 230 235 240 Val Glu Ser Tyr Leu Gln Tyr His Gly Arg Lys Leu Val Glu Arg Phe 245 250 255 Asp Ala Gly Ser Tyr Val Thr Leu Thr Glu Ala Leu Asn Arg His Asn 260 265 270 Ile Gly Arg Gly Arg Gly Gly Leu Asn Lys Ala Leu Ala Ala Cys Thr 275 280 285 Val Pro Thr Met Ile Val Gly Val Asp Thr Asp Ile Leu Tyr Pro Tyr 290 295 300 His Gln Gln Glu His Leu Ser Arg Asn Val Gly Glu Leu Met Gly Ile 305 310 315 320 Ala Arg Ile Ser Ser Pro Val Gly His Asp Ala Phe Leu Ala Glu Phe 325 330 335 Arg Gln Met Asp Arg Ile Leu Arg Arg Phe Phe Val Leu Ser Val Leu 340 345 350 Asp Glu Asp Ala Gln Glu Asp Asp Gly Tyr Leu Ile 355 360 <210> 61 <211> 1872 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1572) <223> gene metY <400> 61 ccaagttgga gcggcgcggc atcaagttcg cgatggcgcg cgtgaagcag gacctctacc 60 gacgcctcga gcccaccggc ttcatcgacc acgtcggccg ggactacatt ttcgagacgc 120 tgcccaccgg ggtgcgggcc tacgccaacc gctaccaggc ccggcacgga cgctggccgg 180 acggggtgcc gcgcgaggtg gtcgagccct gacgcggacg gggtggaccc cgagctcatg 240 cccgcggcgg gtacatttgc attcaggtat tccgtcgccc cgaaaggtgt ctgcatcacc 300 atg cac aac cag acc aag ctc atc cac tcc ggc tat gtc ccg ggc aac 348 Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn 1 5 10 15 aag gac ccc cga cag gtg ccg atc gtg cag tcc acg acc tac acc ttc 396 Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe 20 25 30 gac tcc tcc gag gac atc gcg gcg gtg ttc gat gag ccg acc cac gcg 444 Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala 35 40 45 ctg atc tac tcc cgc ttc gcc aac ccg acc gtc atg gcg gtg gag acg 492 Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr 50 55 60 aag atc gcc gag ctg gag ggc ggt gcc gcg gcg atg gcg acc tcc tcc 540 Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser 65 70 75 80 ggc cag gcc gcg acc gcg ctg tcg atc atg aac gtc tgc tcg gcg ggt 588 Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly 85 90 95 gac tcc ttc gtg gcc tcc tcc gag atc tac ggc ggc acc ctc aac ctc 636 Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu 100 105 110 ttc cac acg acg ctc aag cgt ttc ggc atc gag gtc atc tac gtc gac 684 Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp 115 120 125 cag gac gcc tcc gag gag gag atc gcc gcc gcc ttc aag ccg aac acg 732 Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr 130 135 140 aag gca ctg ttc ggt gag acc atc ggc aac ccg gcg atg agc gtg ctg 780 Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu 145 150 155 160 gac atc gag aag ttc gcc cgc atc gcc cac ggt cag ggc gtg ccg ctc 828 Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu 165 170 175 atc gtc gac tcc acc ttc gcc acc ccg atg ctg tgc cgc ccc atc gag 876 Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu 180 185 190 cac ggc gcc gac gtg gtc atc cac tcc acc tcc aag tac atg gac ggc 924 His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly 195 200 205 cac gcg ctg cag gtc ggc ggc atg atc gtc gac gcc ggc acc ttc gac 972 His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp 210 215 220 tac acc aac ggc aag ttc ccg ggc ttc acc gag ccg gac gag tcc tac 1020 Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr 225 230 235 240 cac ggc gtc gtg tac acc aag gac tac gcc gtt gcc ccc ttc atc atc 1068 His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile 245 250 255 aag gcc cgc atg cag ctg cag cgt gat ttc ggc gcc tac ccg tcc gcg 1116 Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala 260 265 270 cac tcc gcc ttc atg ctc aac cac tcc ctc gag agc ctc gac gtg cgc 1164 His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg 275 280 285 atg cag cgc cac tgc gag aac gca cag agg gtc gcc gag tac ctc gag 1212 Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu 290 295 300 ggc cgc aag gac gtg ctt gtc gac gtc cgc tac ccc gga ctc gac tcc 1260 Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser 305 310 315 320 tcc ccg tac cgc gag ctg gcg aag aag tac ctc agc ggc aac tcc ggc 1308 Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly 325 330 335 gtg ctg tgc atc gac atc aag ggg ggc cgc gag gcc ggc gtg aag ttc 1356 Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe 340 345 350 atg gac gcc ctc gag ctg gtc acc cgc cag gtg cac gtc gcc gac gcc 1404 Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala 355 360 365 cgt tcc tgc gtg ctg cac ccg gcg tcg acc acg cac cgc cag gtg ccg 1452 Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro 370 375 380 gac gag cag ctg ccg tcc gtc ggc atc acc ccg ggt ctg gtg cgc atc 1500 Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile 385 390 395 400 tcc gtc ggc ctg gag aac gtc gac gac atc atc gcc gac atc gag cag 1548 Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln 405 410 415 gcc ctg gag aag tcc cag gcc taa gttcgcttat cgacgccgcc ccggtccctc 1602 Ala Leu Glu Lys Ser Gln Ala 420 atgtgggacc ggggcggtgg ctttcggggg atggctgcac tttcaccccg actgggtgcg 1662 tgtcgacaac ggggtttggg ggacagcgat gagggtataa gtgcagacac ggagaaggcc 1722 cggacactgt gggaggtgtc cgggccttgg gggcgtcgat aagcggtgtc tctagaactc 1782 cggcagcggc agaacagtga gggagaacat cgcgacgatg gccatgacca gcgggatggc 1842 gatcatgatc ggggcggcga tagccgggtt 1872 <210> 62 <211> 423 <212> PRT <213> Corynebacterium humireducens <400> 62 Met His Asn Gln Thr Lys Leu Ile His Ser Gly Tyr Val Pro Gly Asn 1 5 10 15 Lys Asp Pro Arg Gln Val Pro Ile Val Gln Ser Thr Thr Tyr Thr Phe 20 25 30 Asp Ser Ser Glu Asp Ile Ala Ala Val Phe Asp Glu Pro Thr His Ala 35 40 45 Leu Ile Tyr Ser Arg Phe Ala Asn Pro Thr Val Met Ala Val Glu Thr 50 55 60 Lys Ile Ala Glu Leu Glu Gly Gly Ala Ala Ala Met Ala Thr Ser Ser 65 70 75 80 Gly Gln Ala Ala Thr Ala Leu Ser Ile Met Asn Val Cys Ser Ala Gly 85 90 95 Asp Ser Phe Val Ala Ser Ser Glu Ile Tyr Gly Gly Thr Leu Asn Leu 100 105 110 Phe His Thr Thr Leu Lys Arg Phe Gly Ile Glu Val Ile Tyr Val Asp 115 120 125 Gln Asp Ala Ser Glu Glu Glu Ile Ala Ala Ala Phe Lys Pro Asn Thr 130 135 140 Lys Ala Leu Phe Gly Glu Thr Ile Gly Asn Pro Ala Met Ser Val Leu 145 150 155 160 Asp Ile Glu Lys Phe Ala Arg Ile Ala His Gly Gln Gly Val Pro Leu 165 170 175 Ile Val Asp Ser Thr Phe Ala Thr Pro Met Leu Cys Arg Pro Ile Glu 180 185 190 His Gly Ala Asp Val Val Ile His Ser Thr Ser Lys Tyr Met Asp Gly 195 200 205 His Ala Leu Gln Val Gly Gly Met Ile Val Asp Ala Gly Thr Phe Asp 210 215 220 Tyr Thr Asn Gly Lys Phe Pro Gly Phe Thr Glu Pro Asp Glu Ser Tyr 225 230 235 240 His Gly Val Val Tyr Thr Lys Asp Tyr Ala Val Ala Pro Phe Ile Ile 245 250 255 Lys Ala Arg Met Gln Leu Gln Arg Asp Phe Gly Ala Tyr Pro Ser Ala 260 265 270 His Ser Ala Phe Met Leu Asn His Ser Leu Glu Ser Leu Asp Val Arg 275 280 285 Met Gln Arg His Cys Glu Asn Ala Gln Arg Val Ala Glu Tyr Leu Glu 290 295 300 Gly Arg Lys Asp Val Leu Val Asp Val Arg Tyr Pro Gly Leu Asp Ser 305 310 315 320 Ser Pro Tyr Arg Glu Leu Ala Lys Lys Tyr Leu Ser Gly Asn Ser Gly 325 330 335 Val Leu Cys Ile Asp Ile Lys Gly Gly Arg Glu Ala Gly Val Lys Phe 340 345 350 Met Asp Ala Leu Glu Leu Val Thr Arg Gln Val His Val Ala Asp Ala 355 360 365 Arg Ser Cys Val Leu His Pro Ala Ser Thr Thr His Arg Gln Val Pro 370 375 380 Asp Glu Gln Leu Pro Ser Val Gly Ile Thr Pro Gly Leu Val Arg Ile 385 390 395 400 Ser Val Gly Leu Glu Asn Val Asp Asp Ile Ile Ala Asp Ile Glu Gln 405 410 415 Ala Leu Glu Lys Ser Gln Ala 420 <210> 63 <211> 4014 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(3714) <223> gene pyc <400> 63 cggcaccgga ccaccgcgac gcccacggca ctgcccacac attggggtgg tgcgcgtggg 60 tttcgctgtt cggaacacct gtcggcccat tccgcagggc gggacagaaa cggcctttcg 120 ggcgcccgtc ccgcggagtg cggaaatgga ccgcaccggg tccaccacct gcagataccc 180 cctgtgacta ggggatactc ccccgaagac aaccctcttt cttgcgatac tgtggtgtgc 240 cagagagctc acccgctctc agacacaccc ccgacatgac gttcggaaag gaaccccacc 300 gtg gcc aca acc act ctg cca gcc ttc aca aag gtg ctg gtc gcc aac 348 Val Ala Thr Thr Thr Leu Pro Ala Phe Thr Lys Val Leu Val Ala Asn 1 5 10 15 cgc ggc gag atc gcc gtc cgc gcc ttc cgt gcc gcc ttc gag acc ggt 396 Arg Gly Glu Ile Ala Val Arg Ala Phe Arg Ala Ala Phe Glu Thr Gly 20 25 30 gcc tcc acc gtc gcg gtc tac ccg aag gag gac cgc aac tcg cac cac 444 Ala Ser Thr Val Ala Val Tyr Pro Lys Glu Asp Arg Asn Ser His His 35 40 45 cgc tcc ttc gcc tcc gag gcc gtg cgc atc ggc acc gag gga tcg ccg 492 Arg Ser Phe Ala Ser Glu Ala Val Arg Ile Gly Thr Glu Gly Ser Pro 50 55 60 gtc aag gcg tac ctc gac atc gac gag atc atc cgc gcc gcc aag aag 540 Val Lys Ala Tyr Leu Asp Ile Asp Glu Ile Ile Arg Ala Ala Lys Lys 65 70 75 80 acc cac gcc gac gcc gtc tac ccc ggc tac ggt ttc ctc tcc gag aac 588 Thr His Ala Asp Ala Val Tyr Pro Gly Tyr Gly Phe Leu Ser Glu Asn 85 90 95 gcc cag ctc gcc cgc gag tgc gcc gag aac ggc ctc acc ttc atc ggc 636 Ala Gln Leu Ala Arg Glu Cys Ala Glu Asn Gly Leu Thr Phe Ile Gly 100 105 110 ccg ccg ccg gag gtc ctc gac ctg acc ggc gac aag tcc gcc gcc gtc 684 Pro Pro Pro Glu Val Leu Asp Leu Thr Gly Asp Lys Ser Ala Ala Val 115 120 125 gag gcc gcc cgg aag gcc gga ctc ccg gtc ctg cag gac tcc gag ccg 732 Glu Ala Ala Arg Lys Ala Gly Leu Pro Val Leu Gln Asp Ser Glu Pro 130 135 140 tcc acc gac atc gac gag ctc gtc gag atg tcg aag gac ttc acc ttc 780 Ser Thr Asp Ile Asp Glu Leu Val Glu Met Ser Lys Asp Phe Thr Phe 145 150 155 160 ccg atc ttc gtc aag gcc gtc gcc ggt ggc ggt gga cgt ggc atg cgc 828 Pro Ile Phe Val Lys Ala Val Ala Gly Gly Gly Gly Arg Gly Met Arg 165 170 175 ttc atc gag aag ccc gag gac gtc gcc cag ctg gcc acc gag gcc tcc 876 Phe Ile Glu Lys Pro Glu Asp Val Ala Gln Leu Ala Thr Glu Ala Ser 180 185 190 cgc gag gcg gag gcc gcc ttc ggt gac ggt cat gtc tac ctc gag cgt 924 Arg Glu Ala Glu Ala Ala Phe Gly Asp Gly His Val Tyr Leu Glu Arg 195 200 205 gcg gtg ctc aac ccg cag cac atc gag gtc cag atc ctc gcc gac cac 972 Ala Val Leu Asn Pro Gln His Ile Glu Val Gln Ile Leu Ala Asp His 210 215 220 acc ggc gac gtc atc cac ctc tac gag cgt gac tgc tcc ctg cag cgc 1020 Thr Gly Asp Val Ile His Leu Tyr Glu Arg Asp Cys Ser Leu Gln Arg 225 230 235 240 cgc cac cag aag gtc gtg gag atc gcc ccg gcc cag cac ctc gac cca 1068 Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Gln His Leu Asp Pro 245 250 255 gcc ctg cgc gac cag atc tgc gcc gac gcc gtg aag ttc tgc cgc tcc 1116 Ala Leu Arg Asp Gln Ile Cys Ala Asp Ala Val Lys Phe Cys Arg Ser 260 265 270 atc ggc tac gtg tgc gcc ggc acc gtc gag ttc ctc gtc gac gag aac 1164 Ile Gly Tyr Val Cys Ala Gly Thr Val Glu Phe Leu Val Asp Glu Asn 275 280 285 ggc aac cac gtc ttc atc gag atg aac ccg cgc atc cag gtc gag cac 1212 Gly Asn His Val Phe Ile Glu Met Asn Pro Arg Ile Gln Val Glu His 290 295 300 acc atc acc gag gag gtc acc tcc gtc gac ctg gtc aag gcg cag atg 1260 Thr Ile Thr Glu Glu Val Thr Ser Val Asp Leu Val Lys Ala Gln Met 305 310 315 320 aac atc gcc gcc ggc gcg acc ctc aag gac ctc ggc ctc acg cag gag 1308 Asn Ile Ala Ala Gly Ala Thr Leu Lys Asp Leu Gly Leu Thr Gln Glu 325 330 335 tcc atc aag ctc cac ggc gcg gcc ctg cag tgc cgc atc acc acc gag 1356 Ser Ile Lys Leu His Gly Ala Ala Leu Gln Cys Arg Ile Thr Thr Glu 340 345 350 gac ccg gcc aac aac ttc cgc ccg gac acc ggc acc atc acc gcg tac 1404 Asp Pro Ala Asn Asn Phe Arg Pro Asp Thr Gly Thr Ile Thr Ala Tyr 355 360 365 cgc tcc ccg ggt ggc gcg ggc gtg cgt ctc gac ggc gcc gcg tcc ctc 1452 Arg Ser Pro Gly Gly Ala Gly Val Arg Leu Asp Gly Ala Ala Ser Leu 370 375 380 ggc ggc gag atc tcc ccg aac ttc gac tcc ctg ctg gtc aag atg acc 1500 Gly Gly Glu Ile Ser Pro Asn Phe Asp Ser Leu Leu Val Lys Met Thr 385 390 395 400 tgc cgc ggc gcc gac ttc gcc acc gcc gtc gcc cgt gcc cag cgc gcg 1548 Cys Arg Gly Ala Asp Phe Ala Thr Ala Val Ala Arg Ala Gln Arg Ala 405 410 415 ctc aac gag ttc acc atc tcg ggc gtg gcc acc aac atc ggc ttc ctc 1596 Leu Asn Glu Phe Thr Ile Ser Gly Val Ala Thr Asn Ile Gly Phe Leu 420 425 430 cgc gcc ctg ctg cgc gag gag gac ttc cag acc cgc cgc atc tcc acc 1644 Arg Ala Leu Leu Arg Glu Glu Asp Phe Gln Thr Arg Arg Ile Ser Thr 435 440 445 aac ttc atc ggc gag cac ctc cac ctg ctg tcc gcc ccg ccg gcc gac 1692 Asn Phe Ile Gly Glu His Leu His Leu Leu Ser Ala Pro Pro Ala Asp 450 455 460 gac gag gcc ggc cgc atc ctc aac tac ctc gcg gac gtc acc gtc aac 1740 Asp Glu Ala Gly Arg Ile Leu Asn Tyr Leu Ala Asp Val Thr Val Asn 465 470 475 480 cgt ccg cac ggc aag cgc ccc acc gag atc cgt ccg gtg acc aag ctg 1788 Arg Pro His Gly Lys Arg Pro Thr Glu Ile Arg Pro Val Thr Lys Leu 485 490 495 ccg gcc cgc gac gac agc ccg ctg ccg cgc ggt tcc cgc gac gtc ctg 1836 Pro Ala Arg Asp Asp Ser Pro Leu Pro Arg Gly Ser Arg Asp Val Leu 500 505 510 cgt gac ctg ggc ccg cag aag ttc gcc gag cag ctg cgc aac cag gac 1884 Arg Asp Leu Gly Pro Gln Lys Phe Ala Glu Gln Leu Arg Asn Gln Asp 515 520 525 gcc ctg gcc gtc acc gac acc acg ttc cgt gac gcg cac cag tcg ctg 1932 Ala Leu Ala Val Thr Asp Thr Thr Phe Arg Asp Ala His Gln Ser Leu 530 535 540 ctg gcc acc cgc atc cgt tcc tcc gcg ctc gtc gcc gcc gcc gag cac 1980 Leu Ala Thr Arg Ile Arg Ser Ser Ala Leu Val Ala Ala Ala Glu His 545 550 555 560 gtc ggc cgc ctg acc ccg gag ctg ctc tcc gtc gag gcg tgg ggc ggt 2028 Val Gly Arg Leu Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly Gly 565 570 575 gcg acc tac gac gtc gcg atg cgt ttc ctc cac gag gac ccg tgg gag 2076 Ala Thr Tyr Asp Val Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu 580 585 590 cgt ctc gac gac ctg cgc cgc gcc atg ccg aac gtc aac atc cag atg 2124 Arg Leu Asp Asp Leu Arg Arg Ala Met Pro Asn Val Asn Ile Gln Met 595 600 605 ctg ctg cgc ggc cgc aac acc gtc ggc tac acc tcc tac ccg gac agc 2172 Leu Leu Arg Gly Arg Asn Thr Val Gly Tyr Thr Ser Tyr Pro Asp Ser 610 615 620 gtc ggc cag gcc ttc gtc aag gag gcc gcc gac tcc ggc gtc gac atc 2220 Val Gly Gln Ala Phe Val Lys Glu Ala Ala Asp Ser Gly Val Asp Ile 625 630 635 640 ttc cgc atc ttc gac gcc ctc aac gac gtg gac cag atg cgc ccg gcc 2268 Phe Arg Ile Phe Asp Ala Leu Asn Asp Val Asp Gln Met Arg Pro Ala 645 650 655 atc gat gcc gtc ctg gag acc ggc acc acc gtc gcc gag gtc gcc atg 2316 Ile Asp Ala Val Leu Glu Thr Gly Thr Thr Val Ala Glu Val Ala Met 660 665 670 gcc tac tcc ggc aac ctg ctg gac ccg aac gag aag ctc tac acg ctc 2364 Ala Tyr Ser Gly Asn Leu Leu Asp Pro Asn Glu Lys Leu Tyr Thr Leu 675 680 685 gac tac tac ctc cgt ctg gcc gag gag atc gtc gag tcc ggt gcc cac 2412 Asp Tyr Tyr Leu Arg Leu Ala Glu Glu Ile Val Glu Ser Gly Ala His 690 695 700 atc ctg gcc atc aag gac atg gcc ggt ctg ctc cgc ccg acc gcg gcc 2460 Ile Leu Ala Ile Lys Asp Met Ala Gly Leu Leu Arg Pro Thr Ala Ala 705 710 715 720 tcc cgc ctg gtg aag gcg ctg cgc agc aac ttc gac ctg ccg gtg cac 2508 Ser Arg Leu Val Lys Ala Leu Arg Ser Asn Phe Asp Leu Pro Val His 725 730 735 gtg cac acc cac gac acc gcc ggc ggt cag ctg gcg acc tac ctg gcc 2556 Val His Thr His Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Leu Ala 740 745 750 gcc gcg cag gcg ggt gcc gac gcc gtc gac ggc gcc tcc gcc ccg ctg 2604 Ala Ala Gln Ala Gly Ala Asp Ala Val Asp Gly Ala Ser Ala Pro Leu 755 760 765 gcc ggc acg acc tcg cag ccc tcg ctg tcc gcg atc gtc gcg gcc ttc 2652 Ala Gly Thr Thr Ser Gln Pro Ser Leu Ser Ala Ile Val Ala Ala Phe 770 775 780 gcc gac acc cac cgc gac acc ggc ctg tcc ctg cag gcc gtg tcc gac 2700 Ala Asp Thr His Arg Asp Thr Gly Leu Ser Leu Gln Ala Val Ser Asp 785 790 795 800 ctc gag ccc tac tgg gag gcc gtg cgc cag ctc tac gcc ccc ttc gag 2748 Leu Glu Pro Tyr Trp Glu Ala Val Arg Gln Leu Tyr Ala Pro Phe Glu 805 810 815 gcg ggc gtg ccc ggc ccg acg ggc cgc gtg tac aag cac gag atc ccg 2796 Ala Gly Val Pro Gly Pro Thr Gly Arg Val Tyr Lys His Glu Ile Pro 820 825 830 ggt ggt cag ctg tcg aac ctg cgc acg cag gcc aag gcc ctg ggt ctg 2844 Gly Gly Gln Leu Ser Asn Leu Arg Thr Gln Ala Lys Ala Leu Gly Leu 835 840 845 gag gac cgc ttc gag ctc atc gag gac tac tac gcc gcc gtc aac gag 2892 Glu Asp Arg Phe Glu Leu Ile Glu Asp Tyr Tyr Ala Ala Val Asn Glu 850 855 860 atg ctg ggc cgc ccc acc aag gtc acc ccg tcc tcc aag gtc gtc ggc 2940 Met Leu Gly Arg Pro Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly 865 870 875 880 gac ctg gcg ctc cac ctc gtc ggc gcg ggc gtc gac ccg cac gac ttc 2988 Asp Leu Ala Leu His Leu Val Gly Ala Gly Val Asp Pro His Asp Phe 885 890 895 gcc aat gac ccg cag aag tac gac atc ccg gac tcc gtg atc gac ttc 3036 Ala Asn Asp Pro Gln Lys Tyr Asp Ile Pro Asp Ser Val Ile Asp Phe 900 905 910 ctc cgc ggt gac ctc ggc aac ccg ccc ggt ggc tgg ccg atg ctg cgg 3084 Leu Arg Gly Asp Leu Gly Asn Pro Pro Gly Gly Trp Pro Met Leu Arg 915 920 925 gag aag gcc ctg gca ggt cgt tcc tcc acg cag tcc aag ctt gtc gac 3132 Glu Lys Ala Leu Ala Gly Arg Ser Ser Thr Gln Ser Lys Leu Val Asp 930 935 940 gtc gcc ccg gag gac gag gag gcc ctg cag agc gcg gac cgc cag acc 3180 Val Ala Pro Glu Asp Glu Glu Ala Leu Gln Ser Ala Asp Arg Gln Thr 945 950 955 960 cgc cgc tcg acc ctg gac agg ctg ctg ttc ccg aag cag gcc gcc gag 3228 Arg Arg Ser Thr Leu Asp Arg Leu Leu Phe Pro Lys Gln Ala Ala Glu 965 970 975 ttc gcg gag cac cgc cgc cag tac ggc aac acc gcc gcc ctc gag gac 3276 Phe Ala Glu His Arg Arg Gln Tyr Gly Asn Thr Ala Ala Leu Glu Asp 980 985 990 cgc gtg ttc ttc tac ggc ctc aag gag ggc gag gag aac gtc atc cgc 3324 Arg Val Phe Phe Tyr Gly Leu Lys Glu Gly Glu Glu Asn Val Ile Arg 995 1000 1005 ctc atc ggg gag cac ggt cag ccg ccg atg gtc gtg cgt ctc gac 3369 Leu Ile Gly Glu His Gly Gln Pro Pro Met Val Val Arg Leu Asp 1010 1015 1020 gcc gtc ggt gag ccc gac gag aag ggc atg cgc cag gtg gtg acc 3414 Ala Val Gly Glu Pro Asp Glu Lys Gly Met Arg Gln Val Val Thr 1025 1030 1035 aac gtc aac ggc cag atc cgt ccg atg aag gtc cgt gac cgc tcc 3459 Asn Val Asn Gly Gln Ile Arg Pro Met Lys Val Arg Asp Arg Ser 1040 1045 1050 gtc gag tcg gtc acc gcc tcc gcg gag aag gcc gac acc acc aac 3504 Val Glu Ser Val Thr Ala Ser Ala Glu Lys Ala Asp Thr Thr Asn 1055 1060 1065 ccg ggc cac gtc gcc gcg ccg ttc gcg ggt gtc gtc atg gtc acc 3549 Pro Gly His Val Ala Ala Pro Phe Ala Gly Val Val Met Val Thr 1070 1075 1080 gcg aag gtg ggc gac gag gtc aag gcc ggc gac ccg gta gcc gtg 3594 Ala Lys Val Gly Asp Glu Val Lys Ala Gly Asp Pro Val Ala Val 1085 1090 1095 atc gag gcc atg aag atg gag gcc acc atc acc gcc tcc aag gac 3639 Ile Glu Ala Met Lys Met Glu Ala Thr Ile Thr Ala Ser Lys Asp 1100 1105 1110 ggc gtg gtc gac cgc gtc gcc ctg gcc cag gcc acg aag gtg gag 3684 Gly Val Val Asp Arg Val Ala Leu Ala Gln Ala Thr Lys Val Glu 1115 1120 1125 ggc ggc gac ctc atc gtc gtc atc gcc tag ccgcacaacg cgaaagcccc 3734 Gly Gly Asp Leu Ile Val Val Ile Ala 1130 1135 gccactcctt ccgggagtgg cggggcttct ctgtgcgggt gggccgctac ttgatctcga 3794 gcaggacctg gcccttggtc acgccggcac cggcctcgac ggacaggccc tcgaccttgc 3854 ccgacttgtg ggccttgacc gggttctcca tcttcatggc ctcgaggacc acgacgacgt 3914 cgccctcggc gacctcctgg ccgtcctcga cgttgacctt gatgacggtg ccctgcatcg 3974 gggccgccac agcgtcaccg gaggcacctg cggcaccgcc 4014 <210> 64 <211> 1137 <212> PRT <213> Corynebacterium humireducens <400> 64 Val Ala Thr Thr Thr Leu Pro Ala Phe Thr Lys Val Leu Val Ala Asn 1 5 10 15 Arg Gly Glu Ile Ala Val Arg Ala Phe Arg Ala Ala Phe Glu Thr Gly 20 25 30 Ala Ser Thr Val Ala Val Tyr Pro Lys Glu Asp Arg Asn Ser His His 35 40 45 Arg Ser Phe Ala Ser Glu Ala Val Arg Ile Gly Thr Glu Gly Ser Pro 50 55 60 Val Lys Ala Tyr Leu Asp Ile Asp Glu Ile Ile Arg Ala Ala Lys Lys 65 70 75 80 Thr His Ala Asp Ala Val Tyr Pro Gly Tyr Gly Phe Leu Ser Glu Asn 85 90 95 Ala Gln Leu Ala Arg Glu Cys Ala Glu Asn Gly Leu Thr Phe Ile Gly 100 105 110 Pro Pro Pro Glu Val Leu Asp Leu Thr Gly Asp Lys Ser Ala Ala Val 115 120 125 Glu Ala Ala Arg Lys Ala Gly Leu Pro Val Leu Gln Asp Ser Glu Pro 130 135 140 Ser Thr Asp Ile Asp Glu Leu Val Glu Met Ser Lys Asp Phe Thr Phe 145 150 155 160 Pro Ile Phe Val Lys Ala Val Ala Gly Gly Gly Gly Arg Gly Met Arg 165 170 175 Phe Ile Glu Lys Pro Glu Asp Val Ala Gln Leu Ala Thr Glu Ala Ser 180 185 190 Arg Glu Ala Glu Ala Ala Phe Gly Asp Gly His Val Tyr Leu Glu Arg 195 200 205 Ala Val Leu Asn Pro Gln His Ile Glu Val Gln Ile Leu Ala Asp His 210 215 220 Thr Gly Asp Val Ile His Leu Tyr Glu Arg Asp Cys Ser Leu Gln Arg 225 230 235 240 Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Gln His Leu Asp Pro 245 250 255 Ala Leu Arg Asp Gln Ile Cys Ala Asp Ala Val Lys Phe Cys Arg Ser 260 265 270 Ile Gly Tyr Val Cys Ala Gly Thr Val Glu Phe Leu Val Asp Glu Asn 275 280 285 Gly Asn His Val Phe Ile Glu Met Asn Pro Arg Ile Gln Val Glu His 290 295 300 Thr Ile Thr Glu Glu Val Thr Ser Val Asp Leu Val Lys Ala Gln Met 305 310 315 320 Asn Ile Ala Ala Gly Ala Thr Leu Lys Asp Leu Gly Leu Thr Gln Glu 325 330 335 Ser Ile Lys Leu His Gly Ala Ala Leu Gln Cys Arg Ile Thr Thr Glu 340 345 350 Asp Pro Ala Asn Asn Phe Arg Pro Asp Thr Gly Thr Ile Thr Ala Tyr 355 360 365 Arg Ser Pro Gly Gly Ala Gly Val Arg Leu Asp Gly Ala Ala Ser Leu 370 375 380 Gly Gly Glu Ile Ser Pro Asn Phe Asp Ser Leu Leu Val Lys Met Thr 385 390 395 400 Cys Arg Gly Ala Asp Phe Ala Thr Ala Val Ala Arg Ala Gln Arg Ala 405 410 415 Leu Asn Glu Phe Thr Ile Ser Gly Val Ala Thr Asn Ile Gly Phe Leu 420 425 430 Arg Ala Leu Leu Arg Glu Glu Asp Phe Gln Thr Arg Arg Ile Ser Thr 435 440 445 Asn Phe Ile Gly Glu His Leu His Leu Leu Ser Ala Pro Pro Ala Asp 450 455 460 Asp Glu Ala Gly Arg Ile Leu Asn Tyr Leu Ala Asp Val Thr Val Asn 465 470 475 480 Arg Pro His Gly Lys Arg Pro Thr Glu Ile Arg Pro Val Thr Lys Leu 485 490 495 Pro Ala Arg Asp Asp Ser Pro Leu Pro Arg Gly Ser Arg Asp Val Leu 500 505 510 Arg Asp Leu Gly Pro Gln Lys Phe Ala Glu Gln Leu Arg Asn Gln Asp 515 520 525 Ala Leu Ala Val Thr Asp Thr Thr Phe Arg Asp Ala His Gln Ser Leu 530 535 540 Leu Ala Thr Arg Ile Arg Ser Ser Ala Leu Val Ala Ala Ala Glu His 545 550 555 560 Val Gly Arg Leu Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly Gly 565 570 575 Ala Thr Tyr Asp Val Ala Met Arg Phe Leu His Glu Asp Pro Trp Glu 580 585 590 Arg Leu Asp Asp Leu Arg Arg Ala Met Pro Asn Val Asn Ile Gln Met 595 600 605 Leu Leu Arg Gly Arg Asn Thr Val Gly Tyr Thr Ser Tyr Pro Asp Ser 610 615 620 Val Gly Gln Ala Phe Val Lys Glu Ala Ala Asp Ser Gly Val Asp Ile 625 630 635 640 Phe Arg Ile Phe Asp Ala Leu Asn Asp Val Asp Gln Met Arg Pro Ala 645 650 655 Ile Asp Ala Val Leu Glu Thr Gly Thr Thr Val Ala Glu Val Ala Met 660 665 670 Ala Tyr Ser Gly Asn Leu Leu Asp Pro Asn Glu Lys Leu Tyr Thr Leu 675 680 685 Asp Tyr Tyr Leu Arg Leu Ala Glu Glu Ile Val Glu Ser Gly Ala His 690 695 700 Ile Leu Ala Ile Lys Asp Met Ala Gly Leu Leu Arg Pro Thr Ala Ala 705 710 715 720 Ser Arg Leu Val Lys Ala Leu Arg Ser Asn Phe Asp Leu Pro Val His 725 730 735 Val His Thr His Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Leu Ala 740 745 750 Ala Ala Gln Ala Gly Ala Asp Ala Val Asp Gly Ala Ser Ala Pro Leu 755 760 765 Ala Gly Thr Thr Ser Gln Pro Ser Leu Ser Ala Ile Val Ala Ala Phe 770 775 780 Ala Asp Thr His Arg Asp Thr Gly Leu Ser Leu Gln Ala Val Ser Asp 785 790 795 800 Leu Glu Pro Tyr Trp Glu Ala Val Arg Gln Leu Tyr Ala Pro Phe Glu 805 810 815 Ala Gly Val Pro Gly Pro Thr Gly Arg Val Tyr Lys His Glu Ile Pro 820 825 830 Gly Gly Gln Leu Ser Asn Leu Arg Thr Gln Ala Lys Ala Leu Gly Leu 835 840 845 Glu Asp Arg Phe Glu Leu Ile Glu Asp Tyr Tyr Ala Ala Val Asn Glu 850 855 860 Met Leu Gly Arg Pro Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly 865 870 875 880 Asp Leu Ala Leu His Leu Val Gly Ala Gly Val Asp Pro His Asp Phe 885 890 895 Ala Asn Asp Pro Gln Lys Tyr Asp Ile Pro Asp Ser Val Ile Asp Phe 900 905 910 Leu Arg Gly Asp Leu Gly Asn Pro Pro Gly Gly Trp Pro Met Leu Arg 915 920 925 Glu Lys Ala Leu Ala Gly Arg Ser Ser Thr Gln Ser Lys Leu Val Asp 930 935 940 Val Ala Pro Glu Asp Glu Glu Ala Leu Gln Ser Ala Asp Arg Gln Thr 945 950 955 960 Arg Arg Ser Thr Leu Asp Arg Leu Leu Phe Pro Lys Gln Ala Ala Glu 965 970 975 Phe Ala Glu His Arg Arg Gln Tyr Gly Asn Thr Ala Ala Leu Glu Asp 980 985 990 Arg Val Phe Phe Tyr Gly Leu Lys Glu Gly Glu Glu Asn Val Ile Arg 995 1000 1005 Leu Ile Gly Glu His Gly Gln Pro Pro Met Val Val Arg Leu Asp 1010 1015 1020 Ala Val Gly Glu Pro Asp Glu Lys Gly Met Arg Gln Val Val Thr 1025 1030 1035 Asn Val Asn Gly Gln Ile Arg Pro Met Lys Val Arg Asp Arg Ser 1040 1045 1050 Val Glu Ser Val Thr Ala Ser Ala Glu Lys Ala Asp Thr Thr Asn 1055 1060 1065 Pro Gly His Val Ala Ala Pro Phe Ala Gly Val Val Met Val Thr 1070 1075 1080 Ala Lys Val Gly Asp Glu Val Lys Ala Gly Asp Pro Val Ala Val 1085 1090 1095 Ile Glu Ala Met Lys Met Glu Ala Thr Ile Thr Ala Ser Lys Asp 1100 1105 1110 Gly Val Val Asp Arg Val Ala Leu Ala Gln Ala Thr Lys Val Glu 1115 1120 1125 Gly Gly Asp Leu Ile Val Val Ile Ala 1130 1135 <210> 65 <211> 2190 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1890) <223> gene serA <400> 65 cgacttcgcc aacttcctcc gcgaccgccg ttcccgctac gtcggcatgg tcgagtacgc 60 gatggacaag cccgtggtcc gcgacgtcga cgacagtgac tacacggggg gcgaggaggg 120 cccgaacgcg ttcactgact gaacgggggt gaccggtgga gtggtgtgcc tcatatcggg 180 gcagggtatg ggacaaggcg caaccccaca gactaaagtg ttatccgttc ctcgttcctg 240 gttcggtcgg gtgcgctgac cggccccggg gcctcatttt caaatcagga gatatcacac 300 gtg agc aag ccc gtc gtc ctc atc gcc gac aaa ctg gca cct tcc acc 348 Val Ser Lys Pro Val Val Leu Ile Ala Asp Lys Leu Ala Pro Ser Thr 1 5 10 15 gta gat gcg ctc gga gac gcc gtg gag gtg cgc tgg gtc gac ggc ccg 396 Val Asp Ala Leu Gly Asp Ala Val Glu Val Arg Trp Val Asp Gly Pro 20 25 30 aac acc ccg gag ctg ctc gcc gcc gtc ccg gag gca gac gca ctg ctg 444 Asn Thr Pro Glu Leu Leu Ala Ala Val Pro Glu Ala Asp Ala Leu Leu 35 40 45 gtc cgt tcc gcg acc acc gtc acc cgt gag gtc ctc gag gcc gca ccg 492 Val Arg Ser Ala Thr Thr Val Thr Arg Glu Val Leu Glu Ala Ala Pro 50 55 60 aac ctg aag atc gtc ggc cgc gcc ggc gtc ggc ctg gac aac gtc gac 540 Asn Leu Lys Ile Val Gly Arg Ala Gly Val Gly Leu Asp Asn Val Asp 65 70 75 80 atc gac gcc gcc acc gag cgt ggc gtc atg gtc gtc aac gcc ccg acc 588 Ile Asp Ala Ala Thr Glu Arg Gly Val Met Val Val Asn Ala Pro Thr 85 90 95 tcc aac atc cac tcc gcc tgc gag cac gcc atc gcc ctg ctc atg gcc 636 Ser Asn Ile His Ser Ala Cys Glu His Ala Ile Ala Leu Leu Met Ala 100 105 110 acc gtg cgc cag atc ccg gcc gcc gac gcc acc ctg cgt gag ggc gag 684 Thr Val Arg Gln Ile Pro Ala Ala Asp Ala Thr Leu Arg Glu Gly Glu 115 120 125 tgg aag cgt tcc gcc ttc aag ggc gtg gag atc ttc ggc aag acc atc 732 Trp Lys Arg Ser Ala Phe Lys Gly Val Glu Ile Phe Gly Lys Thr Ile 130 135 140 ggc atc gtc ggc ttc ggc cac atc ggc cag ctc ttc gcc cag cgt ctg 780 Gly Ile Val Gly Phe Gly His Ile Gly Gln Leu Phe Ala Gln Arg Leu 145 150 155 160 tcc gcc ttc gag acc gag atc atc gcc tac gac ccg tac gcg aac ccg 828 Ser Ala Phe Glu Thr Glu Ile Ile Ala Tyr Asp Pro Tyr Ala Asn Pro 165 170 175 acc cgc gcc gcc cag ctc ggc gtc gag ctg gtc gag ctg gag gag ctg 876 Thr Arg Ala Ala Gln Leu Gly Val Glu Leu Val Glu Leu Glu Glu Leu 180 185 190 gtc tcc cgc gcc gac atc gtc acc atc cac ctt ccg aag acc aag gag 924 Val Ser Arg Ala Asp Ile Val Thr Ile His Leu Pro Lys Thr Lys Glu 195 200 205 acg gcc ggc atg ttc aac gcc gag ctg ctc gcc aag tcc aag aag ggc 972 Thr Ala Gly Met Phe Asn Ala Glu Leu Leu Ala Lys Ser Lys Lys Gly 210 215 220 cag atc atc atc aac gcc gcc cgc ggt ggc ctc gtc gat gag cag gca 1020 Gln Ile Ile Ile Asn Ala Ala Arg Gly Gly Leu Val Asp Glu Gln Ala 225 230 235 240 ctg gct gac gcc atc aag tcc ggc cac atc tgg ggc gcc ggc ttc gac 1068 Leu Ala Asp Ala Ile Lys Ser Gly His Ile Trp Gly Ala Gly Phe Asp 245 250 255 gtc ttc tcc acc gag ccg tgc acc gac tcc ccg ctg ttc ggc ctg ccg 1116 Val Phe Ser Thr Glu Pro Cys Thr Asp Ser Pro Leu Phe Gly Leu Pro 260 265 270 gag gtc gtc gtg acc ccg cac ctg ggt gcc tcc acc gaa gag gcc cag 1164 Glu Val Val Val Thr Pro His Leu Gly Ala Ser Thr Glu Glu Ala Gln 275 280 285 gac cgt gcc ggc acc gac gtc gcc gcc tcc gtc ctc aag gcc ctg gcc 1212 Asp Arg Ala Gly Thr Asp Val Ala Ala Ser Val Leu Lys Ala Leu Ala 290 295 300 ggc gag ttc gtc ccg gac gcc gtg aac gtc tcc ggc ggc cgc gtc ggc 1260 Gly Glu Phe Val Pro Asp Ala Val Asn Val Ser Gly Gly Arg Val Gly 305 310 315 320 gag gag gtc gcc ctg tgg ttg gac ctg acc cgc aag ctg ggt ctg ctg 1308 Glu Glu Val Ala Leu Trp Leu Asp Leu Thr Arg Lys Leu Gly Leu Leu 325 330 335 gcc ggt cag ctg ctg gac tcc gcc ccg gtc gac ctc gag gtc gag gcc 1356 Ala Gly Gln Leu Leu Asp Ser Ala Pro Val Asp Leu Glu Val Glu Ala 340 345 350 cgc ggc gag ctc tcc acc gag gac gac ctg tcc gtg ctc ggc ctg tcc 1404 Arg Gly Glu Leu Ser Thr Glu Asp Asp Leu Ser Val Leu Gly Leu Ser 355 360 365 gcc gtc cgt ggc ctg ttc tcc gcc acc acc gac gag ccg gtc acc ttc 1452 Ala Val Arg Gly Leu Phe Ser Ala Thr Thr Asp Glu Pro Val Thr Phe 370 375 380 gtc aac gcc ccg cag atc gcg gag agc cgt ggc ctg gac tac aag gtc 1500 Val Asn Ala Pro Gln Ile Ala Glu Ser Arg Gly Leu Asp Tyr Lys Val 385 390 395 400 gac acc gcg acc gag tcc acc acc cac cgt tcc tcc gtc gag gtc aag 1548 Asp Thr Ala Thr Glu Ser Thr Thr His Arg Ser Ser Val Glu Val Lys 405 410 415 gtc atc ggt gcg gac ggc tcc tcc cgc tcc gtc gtc ggt gcc ctg acc 1596 Val Ile Gly Ala Asp Gly Ser Ser Arg Ser Val Val Gly Ala Leu Thr 420 425 430 ggc ctg gag cgc gtc gag aag atc gtg cgc atc gac gag cgc ggc ctg 1644 Gly Leu Glu Arg Val Glu Lys Ile Val Arg Ile Asp Glu Arg Gly Leu 435 440 445 gac atg cgc gcc gag ggt cgc aac ctc ttc ctc gag tac acc gac gcc 1692 Asp Met Arg Ala Glu Gly Arg Asn Leu Phe Leu Glu Tyr Thr Asp Ala 450 455 460 ccg ggc gcc ctg ggc aag gtg ggc tcc cag ctg ggc gac gcc ggc atc 1740 Pro Gly Ala Leu Gly Lys Val Gly Ser Gln Leu Gly Asp Ala Gly Ile 465 470 475 480 aac atc gag gcc gcc gcc ctc acc cag gcc acc aag ggt gac ggc gcc 1788 Asn Ile Glu Ala Ala Ala Leu Thr Gln Ala Thr Lys Gly Asp Gly Ala 485 490 495 atc ctg atc ctg cgt gtc gac gca gag atc ccg gag gca ctg cag aag 1836 Ile Leu Ile Leu Arg Val Asp Ala Glu Ile Pro Glu Ala Leu Gln Lys 500 505 510 cag atc gcc gag tcc ctg ggt gcc acc aac gat atc cag ctc aac ctg 1884 Gln Ile Ala Glu Ser Leu Gly Ala Thr Asn Asp Ile Gln Leu Asn Leu 515 520 525 gac tag tctccggtcg ccccgtcgac ggcgtgtcgc gtcggtccct cgcggatcgc 1940 Asp gcggcacgcc gttcgcgttt ctgcgggagg cagtttcatt catgccgcct gaccgggaaa 2000 tgtgcaggta gacagtatgg ttcattccag gcgcgccgtt aatagaccga acggtatgga 2060 ctaactagac ggggaagtgt tgaatgggtc aggcaacgaa ccgactgatc caggagtacc 2120 ccgtgtccga cctcaccccg aaccacaccg ccggcgaacc gctcgagccc atcgacgcgc 2180 agaagctcgc 2190 <210> 66 <211> 529 <212> PRT <213> Corynebacterium humireducens <400> 66 Val Ser Lys Pro Val Val Leu Ile Ala Asp Lys Leu Ala Pro Ser Thr 1 5 10 15 Val Asp Ala Leu Gly Asp Ala Val Glu Val Arg Trp Val Asp Gly Pro 20 25 30 Asn Thr Pro Glu Leu Leu Ala Ala Val Pro Glu Ala Asp Ala Leu Leu 35 40 45 Val Arg Ser Ala Thr Thr Val Thr Arg Glu Val Leu Glu Ala Ala Pro 50 55 60 Asn Leu Lys Ile Val Gly Arg Ala Gly Val Gly Leu Asp Asn Val Asp 65 70 75 80 Ile Asp Ala Ala Thr Glu Arg Gly Val Met Val Val Asn Ala Pro Thr 85 90 95 Ser Asn Ile His Ser Ala Cys Glu His Ala Ile Ala Leu Leu Met Ala 100 105 110 Thr Val Arg Gln Ile Pro Ala Ala Asp Ala Thr Leu Arg Glu Gly Glu 115 120 125 Trp Lys Arg Ser Ala Phe Lys Gly Val Glu Ile Phe Gly Lys Thr Ile 130 135 140 Gly Ile Val Gly Phe Gly His Ile Gly Gln Leu Phe Ala Gln Arg Leu 145 150 155 160 Ser Ala Phe Glu Thr Glu Ile Ile Ala Tyr Asp Pro Tyr Ala Asn Pro 165 170 175 Thr Arg Ala Ala Gln Leu Gly Val Glu Leu Val Glu Leu Glu Glu Leu 180 185 190 Val Ser Arg Ala Asp Ile Val Thr Ile His Leu Pro Lys Thr Lys Glu 195 200 205 Thr Ala Gly Met Phe Asn Ala Glu Leu Leu Ala Lys Ser Lys Lys Gly 210 215 220 Gln Ile Ile Ile Asn Ala Ala Arg Gly Gly Leu Val Asp Glu Gln Ala 225 230 235 240 Leu Ala Asp Ala Ile Lys Ser Gly His Ile Trp Gly Ala Gly Phe Asp 245 250 255 Val Phe Ser Thr Glu Pro Cys Thr Asp Ser Pro Leu Phe Gly Leu Pro 260 265 270 Glu Val Val Val Thr Pro His Leu Gly Ala Ser Thr Glu Glu Ala Gln 275 280 285 Asp Arg Ala Gly Thr Asp Val Ala Ala Ser Val Leu Lys Ala Leu Ala 290 295 300 Gly Glu Phe Val Pro Asp Ala Val Asn Val Ser Gly Gly Arg Val Gly 305 310 315 320 Glu Glu Val Ala Leu Trp Leu Asp Leu Thr Arg Lys Leu Gly Leu Leu 325 330 335 Ala Gly Gln Leu Leu Asp Ser Ala Pro Val Asp Leu Glu Val Glu Ala 340 345 350 Arg Gly Glu Leu Ser Thr Glu Asp Asp Leu Ser Val Leu Gly Leu Ser 355 360 365 Ala Val Arg Gly Leu Phe Ser Ala Thr Thr Asp Glu Pro Val Thr Phe 370 375 380 Val Asn Ala Pro Gln Ile Ala Glu Ser Arg Gly Leu Asp Tyr Lys Val 385 390 395 400 Asp Thr Ala Thr Glu Ser Thr Thr His Arg Ser Ser Val Glu Val Lys 405 410 415 Val Ile Gly Ala Asp Gly Ser Ser Arg Ser Val Val Gly Ala Leu Thr 420 425 430 Gly Leu Glu Arg Val Glu Lys Ile Val Arg Ile Asp Glu Arg Gly Leu 435 440 445 Asp Met Arg Ala Glu Gly Arg Asn Leu Phe Leu Glu Tyr Thr Asp Ala 450 455 460 Pro Gly Ala Leu Gly Lys Val Gly Ser Gln Leu Gly Asp Ala Gly Ile 465 470 475 480 Asn Ile Glu Ala Ala Ala Leu Thr Gln Ala Thr Lys Gly Asp Gly Ala 485 490 495 Ile Leu Ile Leu Arg Val Asp Ala Glu Ile Pro Glu Ala Leu Gln Lys 500 505 510 Gln Ile Ala Glu Ser Leu Gly Ala Thr Asn Asp Ile Gln Leu Asn Leu 515 520 525 Asp <210> 67 <211> 1848 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1548) <223> gene serB <400> 67 cgtatccgct ccgagcgccc ggcgttcgag ctgcactacc cgcacatggt tgagcgcatg 60 cgtcgcgagg cacacaccgg tgcccacgac gagcgcgcgg agatcaccca gactccggct 120 ccggccgagg tccgctaaaa gggtttgaca tagacccctc cgactgacac gacccccggt 180 agatccctct accgggggtt gcggcatgtt cgaaaccccc tcccatgaaa gaatgatccc 240 gtggcagatc aagctcagaa ccccgatacc gccgacaccg aagcacagca gcagctgcag 300 gtg gcg ctc cag ccc gga ctg gag tcc gca gtc atc acc ctc acc ggc 348 Val Ala Leu Gln Pro Gly Leu Glu Ser Ala Val Ile Thr Leu Thr Gly 1 5 10 15 cgt gac cgg ccg ggc gtg acg gcc gcc ttc ttc cgc gtc ctg tcc gct 396 Arg Asp Arg Pro Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala 20 25 30 cac gac gtc cag ctt ctc gac gtc gag cag tcc cag ttc cgt gga tac 444 His Asp Val Gln Leu Leu Asp Val Glu Gln Ser Gln Phe Arg Gly Tyr 35 40 45 ctc tcc ctc gcc gcc tac gtg ggc gtc gac ccg gag cgc gtc gac cgt 492 Leu Ser Leu Ala Ala Tyr Val Gly Val Asp Pro Glu Arg Val Asp Arg 50 55 60 ctc cgc gag ggc ctg acc gag acc ctc aag ggc cac ggg cag ctc gcc 540 Leu Arg Glu Gly Leu Thr Glu Thr Leu Lys Gly His Gly Gln Leu Ala 65 70 75 80 acc gtc gag atg gtc ccg gag ggc cag cac tcc agc ccg cgg tcc acg 588 Thr Val Glu Met Val Pro Glu Gly Gln His Ser Ser Pro Arg Ser Thr 85 90 95 cac gtg gcc gtc gtc ctg ggc aac ccg gtg acc gcc acc gac gtg tcg 636 His Val Ala Val Val Leu Gly Asn Pro Val Thr Ala Thr Asp Val Ser 100 105 110 gcc atc ggt cag acg ctg gcc aac tac ggc gcg aac atc gac cgt atc 684 Ala Ile Gly Gln Thr Leu Ala Asn Tyr Gly Ala Asn Ile Asp Arg Ile 115 120 125 cgc ggc atc gcg gac tac ccg atc acc ggc ctg gag atc atg atc acg 732 Arg Gly Ile Ala Asp Tyr Pro Ile Thr Gly Leu Glu Ile Met Ile Thr 130 135 140 atc gcc aac ccg gag ccg ggc ggc ggc aag gcc atg cgt aag gca ctg 780 Ile Ala Asn Pro Glu Pro Gly Gly Gly Lys Ala Met Arg Lys Ala Leu 145 150 155 160 gcg gag ctg acc acc gag ctg ggc gtc gac atc gcc atc gag cgt gcc 828 Ala Glu Leu Thr Thr Glu Leu Gly Val Asp Ile Ala Ile Glu Arg Ala 165 170 175 ggc ctg ctg cgc cgc tcc aag cgt ctg gtc atc ttc gac tgc gac tcc 876 Gly Leu Leu Arg Arg Ser Lys Arg Leu Val Ile Phe Asp Cys Asp Ser 180 185 190 acc ctc atc acc ggt gag gtc atc gag atg ctg gcg gcc cac gcc ggc 924 Thr Leu Ile Thr Gly Glu Val Ile Glu Met Leu Ala Ala His Ala Gly 195 200 205 aag gag gcc gag gtc gcc gag gtc acc gag cgg gcc atg cgc ggc gag 972 Lys Glu Ala Glu Val Ala Glu Val Thr Glu Arg Ala Met Arg Gly Glu 210 215 220 ctc gac ttc gag gag tcc ctc cgt gag cgc gtc gcc acc ctc gag ggt 1020 Leu Asp Phe Glu Glu Ser Leu Arg Glu Arg Val Ala Thr Leu Glu Gly 225 230 235 240 ctc gac gcc tcc gtc atc gac gag gtc gcc cgc gac atc gtc ctg acc 1068 Leu Asp Ala Ser Val Ile Asp Glu Val Ala Arg Asp Ile Val Leu Thr 245 250 255 ccg ggc gcc cgc acg acc atc cgc acc ctg aag aag atg ggc tac cgc 1116 Pro Gly Ala Arg Thr Thr Ile Arg Thr Leu Lys Lys Met Gly Tyr Arg 260 265 270 acg gcc gtc gtc tcc ggt ggc ttc atc cag gtc ctc gag gac ctg gcc 1164 Thr Ala Val Val Ser Gly Gly Phe Ile Gln Val Leu Glu Asp Leu Ala 275 280 285 gag gac ctc cag ctc gac tac gtc cgc gcg aac acc ctg gag atc gtc 1212 Glu Asp Leu Gln Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile Val 290 295 300 gac ggc aag ctc acc ggc cgc gtg acc ggt gac atc gtc gac cgt gcc 1260 Asp Gly Lys Leu Thr Gly Arg Val Thr Gly Asp Ile Val Asp Arg Ala 305 310 315 320 cgc aag gcg gag ctg ctg cgc gag ttc gcc gag gac tcc ggc ctg cag 1308 Arg Lys Ala Glu Leu Leu Arg Glu Phe Ala Glu Asp Ser Gly Leu Gln 325 330 335 atg tac cag acc gtg gcc gtg ggt gac ggt gcc aac gac atc gac atg 1356 Met Tyr Gln Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met 340 345 350 ctc tcc gcg gcg ggc ctg ggc atc gcg ttc aac gcc aag ccg gcg ctg 1404 Leu Ser Ala Ala Gly Leu Gly Ile Ala Phe Asn Ala Lys Pro Ala Leu 355 360 365 cgc gag atc gcc gac acc tcc gtc aac cac ccc ttc ctc gac gag gtg 1452 Arg Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu Val 370 375 380 ctc cac atc ctg ggc gtc ccg cgc gag gag atc gac gag tcc gac ctg 1500 Leu His Ile Leu Gly Val Pro Arg Glu Glu Ile Asp Glu Ser Asp Leu 385 390 395 400 gag gac ggc acc ttc cgc cgg gtc ccg ctc gag gtc tcc gca cgg tag 1548 Glu Asp Gly Thr Phe Arg Arg Val Pro Leu Glu Val Ser Ala Arg 405 410 415 tggaggagac cgatccgctc gccctcgcgc acgccctcct gagcgcgcgg gtgagcgaca 1608 accggcacga ccgctccgag aacccggagc agcaggagac cgtgcaggcg atgcagatcg 1668 ccctgcacgt gccgaagaca caaccgcccc gccgtaccga cctcctcgag gccgcggcgc 1728 gggcggttgt cgccgtctgc ctctccgaca gggtcgcggg ggacccgtcc tggcgggcgg 1788 ggctggaggg ctggtacgac cacctcatcc gcaaggtgac ccgccgggcc cgcaacaagg 1848 <210> 68 <211> 415 <212> PRT <213> Corynebacterium humireducens <400> 68 Val Ala Leu Gln Pro Gly Leu Glu Ser Ala Val Ile Thr Leu Thr Gly 1 5 10 15 Arg Asp Arg Pro Gly Val Thr Ala Ala Phe Phe Arg Val Leu Ser Ala 20 25 30 His Asp Val Gln Leu Leu Asp Val Glu Gln Ser Gln Phe Arg Gly Tyr 35 40 45 Leu Ser Leu Ala Ala Tyr Val Gly Val Asp Pro Glu Arg Val Asp Arg 50 55 60 Leu Arg Glu Gly Leu Thr Glu Thr Leu Lys Gly His Gly Gln Leu Ala 65 70 75 80 Thr Val Glu Met Val Pro Glu Gly Gln His Ser Ser Pro Arg Ser Thr 85 90 95 His Val Ala Val Val Leu Gly Asn Pro Val Thr Ala Thr Asp Val Ser 100 105 110 Ala Ile Gly Gln Thr Leu Ala Asn Tyr Gly Ala Asn Ile Asp Arg Ile 115 120 125 Arg Gly Ile Ala Asp Tyr Pro Ile Thr Gly Leu Glu Ile Met Ile Thr 130 135 140 Ile Ala Asn Pro Glu Pro Gly Gly Gly Lys Ala Met Arg Lys Ala Leu 145 150 155 160 Ala Glu Leu Thr Thr Glu Leu Gly Val Asp Ile Ala Ile Glu Arg Ala 165 170 175 Gly Leu Leu Arg Arg Ser Lys Arg Leu Val Ile Phe Asp Cys Asp Ser 180 185 190 Thr Leu Ile Thr Gly Glu Val Ile Glu Met Leu Ala Ala His Ala Gly 195 200 205 Lys Glu Ala Glu Val Ala Glu Val Thr Glu Arg Ala Met Arg Gly Glu 210 215 220 Leu Asp Phe Glu Glu Ser Leu Arg Glu Arg Val Ala Thr Leu Glu Gly 225 230 235 240 Leu Asp Ala Ser Val Ile Asp Glu Val Ala Arg Asp Ile Val Leu Thr 245 250 255 Pro Gly Ala Arg Thr Thr Ile Arg Thr Leu Lys Lys Met Gly Tyr Arg 260 265 270 Thr Ala Val Val Ser Gly Gly Phe Ile Gln Val Leu Glu Asp Leu Ala 275 280 285 Glu Asp Leu Gln Leu Asp Tyr Val Arg Ala Asn Thr Leu Glu Ile Val 290 295 300 Asp Gly Lys Leu Thr Gly Arg Val Thr Gly Asp Ile Val Asp Arg Ala 305 310 315 320 Arg Lys Ala Glu Leu Leu Arg Glu Phe Ala Glu Asp Ser Gly Leu Gln 325 330 335 Met Tyr Gln Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met 340 345 350 Leu Ser Ala Ala Gly Leu Gly Ile Ala Phe Asn Ala Lys Pro Ala Leu 355 360 365 Arg Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Glu Val 370 375 380 Leu His Ile Leu Gly Val Pro Arg Glu Glu Ile Asp Glu Ser Asp Leu 385 390 395 400 Glu Asp Gly Thr Phe Arg Arg Val Pro Leu Glu Val Ser Ala Arg 405 410 415 <210> 69 <211> 1731 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1431) <223> gene serC <400> 69 ctgtgcagtt gtgctgccac cgcacggtgt ttgcgctgct cccttggggg gagggccgcc 60 gtccatgagg tggaacaggg cacagtttag caaatgatgt gatggaggca acagatttca 120 gaaaagcagg tcacccccat tgaatttgcg aggataagcc cctttgccta ccccagcaac 180 tacccccgtt gtcggtgtca accctaacac ccgggttccg gtcaacgggt gggaggggag 240 atactaaggt tgttctcgat tggacagcac ttcaccgacc acgaaaggga cgtcatctcc 300 atg acc gag ttc ccc acc ctc ccc gca gat ctc ctc ccg gca gac ggt 348 Met Thr Glu Phe Pro Thr Leu Pro Ala Asp Leu Leu Pro Ala Asp Gly 1 5 10 15 cgt ttc ggc tgc ggc ccc tcc aag gtc cgc ccg gag cag atc cag gcc 396 Arg Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu Gln Ile Gln Ala 20 25 30 atc gtc gac ggc ggc cgc gac atc atc ggc acc tcg cac cgc cag ccg 444 Ile Val Asp Gly Gly Arg Asp Ile Ile Gly Thr Ser His Arg Gln Pro 35 40 45 gcc gtg aag aat gtc gtc ggt tcg gtc cgt gag ggc ctc tcc gac ctg 492 Ala Val Lys Asn Val Val Gly Ser Val Arg Glu Gly Leu Ser Asp Leu 50 55 60 ttc tcc ctg ccg gag ggc tac gag atc atc ctg tcc ctc ggt ggc gcc 540 Phe Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser Leu Gly Gly Ala 65 70 75 80 acc gca ttc tgg gac gcc gcc acc ttc ggc ctc atc gag aag aag tcc 588 Thr Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile Glu Lys Lys Ser 85 90 95 ggt cac ctg tcc tac ggt gag ttc tcc tcc aag ttc gcc aag gcc tcc 636 Gly His Leu Ser Tyr Gly Glu Phe Ser Ser Lys Phe Ala Lys Ala Ser 100 105 110 cag cag gca ccg tgg ctg gat gag ccg acc atc atc gag gcc ccg gcc 684 Gln Gln Ala Pro Trp Leu Asp Glu Pro Thr Ile Ile Glu Ala Pro Ala 115 120 125 ggc gac gcc ccg gag ccg cag gcc atg gag ggc acc gac gtc atc ggc 732 Gly Asp Ala Pro Glu Pro Gln Ala Met Glu Gly Thr Asp Val Ile Gly 130 135 140 tgg gcc cac aac gag acc tcc acc ggc gcc atg gtg ccg atc gtc cgc 780 Trp Ala His Asn Glu Thr Ser Thr Gly Ala Met Val Pro Ile Val Arg 145 150 155 160 ccg gag ggc tcc gac ggc tcc ctc atc gtc atc gac gcc acc tcc ggc 828 Pro Glu Gly Ser Asp Gly Ser Leu Ile Val Ile Asp Ala Thr Ser Gly 165 170 175 gcc ggc ggc ctc ccg gtg aac atc gcc gac acc gac gtc tac tac ttc 876 Ala Gly Gly Leu Pro Val Asn Ile Ala Asp Thr Asp Val Tyr Tyr Phe 180 185 190 tcc ccg cag aag tgc ttc gcc tcc gac ggt ggc ctg tgg ctc gcc gcc 924 Ser Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu Trp Leu Ala Ala 195 200 205 atg tcc ccg gcc gcc atg gag cgc atc gcg aag atc aag gag tcc ggc 972 Met Ser Pro Ala Ala Met Glu Arg Ile Ala Lys Ile Lys Glu Ser Gly 210 215 220 cgc cac atc ccg gcg ttc ctg gac ctg cag acc gcc gtc gag aac tcc 1020 Arg His Ile Pro Ala Phe Leu Asp Leu Gln Thr Ala Val Glu Asn Ser 225 230 235 240 ctg aag aac cag acc tac aac acc ccg gcc gtg ggc acc ctg ctc atg 1068 Leu Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Gly Thr Leu Leu Met 245 250 255 ctg gac aac cag gtc aag tgg atg aac gag aac ggt ggc ctg gac ggc 1116 Leu Asp Asn Gln Val Lys Trp Met Asn Glu Asn Gly Gly Leu Asp Gly 260 265 270 atg gtc gcc cgc acc acc gcc tcc tcc gac gcc ctg tac aac tgg gcc 1164 Met Val Ala Arg Thr Thr Ala Ser Ser Asp Ala Leu Tyr Asn Trp Ala 275 280 285 gag gcc cgc gag gag acc acc ccg tac gtc acc gac aag gcg aag cgc 1212 Glu Ala Arg Glu Glu Thr Thr Pro Tyr Val Thr Asp Lys Ala Lys Arg 290 295 300 tcc ctc gtc gtc ggc acc atc gac ttc gac gac tcc gtc gac gcg gcg 1260 Ser Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser Val Asp Ala Ala 305 310 315 320 gtc gtc gcc aag gtc ctg cgt gcc aac ggc atc ctc gac gtc gag ccc 1308 Val Val Ala Lys Val Leu Arg Ala Asn Gly Ile Leu Asp Val Glu Pro 325 330 335 tac cgc aag ctg ggc cgc aac cag ctg cgc atc ggc atg ttc ccg gcg 1356 Tyr Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly Met Phe Pro Ala 340 345 350 atc gac tcc cag gac atc gtg acc ctc acc aag gcg atc gac cac gtc 1404 Ile Asp Ser Gln Asp Ile Val Thr Leu Thr Lys Ala Ile Asp His Val 355 360 365 ctc gac tcg ggc gtc gcc gcc aag tag tcgccaggta gtcgcgggca 1451 Leu Asp Ser Gly Val Ala Ala Lys 370 375 cccgcacacc acccaacatg cgtcggacct cacacggtcc ggcgcatgtc tgcgttaggg 1511 tggtattcat cacgagagaa ggagaacgca tgcgcgagtt gttcctcgtc cctgaagagt 1571 cctcggccac ttccctggtg ttccggaccg aggacggcgg acagttcttc ctcgacgtca 1631 cttcactgga cgtggcctcc acccgtgcgc tcctgcttgc cgacgtcccg gaggacccct 1691 cccccacgga gtccggcacc gccgccgtca ccgccgtcga 1731 <210> 70 <211> 376 <212> PRT <213> Corynebacterium humireducens <400> 70 Met Thr Glu Phe Pro Thr Leu Pro Ala Asp Leu Leu Pro Ala Asp Gly 1 5 10 15 Arg Phe Gly Cys Gly Pro Ser Lys Val Arg Pro Glu Gln Ile Gln Ala 20 25 30 Ile Val Asp Gly Gly Arg Asp Ile Ile Gly Thr Ser His Arg Gln Pro 35 40 45 Ala Val Lys Asn Val Val Gly Ser Val Arg Glu Gly Leu Ser Asp Leu 50 55 60 Phe Ser Leu Pro Glu Gly Tyr Glu Ile Ile Leu Ser Leu Gly Gly Ala 65 70 75 80 Thr Ala Phe Trp Asp Ala Ala Thr Phe Gly Leu Ile Glu Lys Lys Ser 85 90 95 Gly His Leu Ser Tyr Gly Glu Phe Ser Ser Lys Phe Ala Lys Ala Ser 100 105 110 Gln Gln Ala Pro Trp Leu Asp Glu Pro Thr Ile Ile Glu Ala Pro Ala 115 120 125 Gly Asp Ala Pro Glu Pro Gln Ala Met Glu Gly Thr Asp Val Ile Gly 130 135 140 Trp Ala His Asn Glu Thr Ser Thr Gly Ala Met Val Pro Ile Val Arg 145 150 155 160 Pro Glu Gly Ser Asp Gly Ser Leu Ile Val Ile Asp Ala Thr Ser Gly 165 170 175 Ala Gly Gly Leu Pro Val Asn Ile Ala Asp Thr Asp Val Tyr Tyr Phe 180 185 190 Ser Pro Gln Lys Cys Phe Ala Ser Asp Gly Gly Leu Trp Leu Ala Ala 195 200 205 Met Ser Pro Ala Ala Met Glu Arg Ile Ala Lys Ile Lys Glu Ser Gly 210 215 220 Arg His Ile Pro Ala Phe Leu Asp Leu Gln Thr Ala Val Glu Asn Ser 225 230 235 240 Leu Lys Asn Gln Thr Tyr Asn Thr Pro Ala Val Gly Thr Leu Leu Met 245 250 255 Leu Asp Asn Gln Val Lys Trp Met Asn Glu Asn Gly Gly Leu Asp Gly 260 265 270 Met Val Ala Arg Thr Thr Ala Ser Ser Asp Ala Leu Tyr Asn Trp Ala 275 280 285 Glu Ala Arg Glu Glu Thr Thr Pro Tyr Val Thr Asp Lys Ala Lys Arg 290 295 300 Ser Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser Val Asp Ala Ala 305 310 315 320 Val Val Ala Lys Val Leu Arg Ala Asn Gly Ile Leu Asp Val Glu Pro 325 330 335 Tyr Arg Lys Leu Gly Arg Asn Gln Leu Arg Ile Gly Met Phe Pro Ala 340 345 350 Ile Asp Ser Gln Asp Ile Val Thr Leu Thr Lys Ala Ile Asp His Val 355 360 365 Leu Asp Ser Gly Val Ala Ala Lys 370 375 <210> 71 <211> 1665 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1365) <223> gene Alanine-Dehydrogenase <400> 71 acctctcgct cagccacctg cgccgcgcga cgaactcctc gcggaagtag ccgaggtggg 60 cggtcggccc gccgaaggcg gtgacgccca gcagcccgaa gctgcggatg acctctcctg 120 tggtgccgtt gttcttgtct gcggattcct tcacgctcgt catggtaggg cggggcgtcg 180 agaagtgcgc gtcgagcgac agtaaccggg attacatcat taaccagccg gttccgacga 240 gcgtccggaa ggcgcctagt agagtcagtg gtcatgcgta tcggaatccc gaaggaaatc 300 atg aac atg gag ggc cgc gtg gcc ctc agc ccg ctc ggg gca ggc acc 348 Met Asn Met Glu Gly Arg Val Ala Leu Ser Pro Leu Gly Ala Gly Thr 1 5 10 15 ctc gta gcc gac ggc cat gag gtg att gtt cag gcc ggg gcg ggc gag 396 Leu Val Ala Asp Gly His Glu Val Ile Val Gln Ala Gly Ala Gly Glu 20 25 30 aac tcc tcg ttc acc gat gcc gag tac gag gcg acg ggc gcg cgc gtc 444 Asn Ser Ser Phe Thr Asp Ala Glu Tyr Glu Ala Thr Gly Ala Arg Val 35 40 45 gtc gcc tcc gcg gag gag gcc tgg ggc gcc gag atg gtg ctc aag gtc 492 Val Ala Ser Ala Glu Glu Ala Trp Gly Ala Glu Met Val Leu Lys Val 50 55 60 aag gag ccg ctg ccg gag gag tac ggc ttc ctc cgc ccg gac ctc atc 540 Lys Glu Pro Leu Pro Glu Glu Tyr Gly Phe Leu Arg Pro Asp Leu Ile 65 70 75 80 ctg ttc acc tac ctg cac ctg gcg tcc tcg cgt gag tgc acg aag gcg 588 Leu Phe Thr Tyr Leu His Leu Ala Ser Ser Arg Glu Cys Thr Lys Ala 85 90 95 ctg ctc gac gcc ggc acc ctg tcc ctg gcg tac gag acg gtc ctc gac 636 Leu Leu Asp Ala Gly Thr Leu Ser Leu Ala Tyr Glu Thr Val Leu Asp 100 105 110 gcc cgc ggc ggc ctc ccg ctg ctc acc ccg atg tcc cag gtc gcg ggc 684 Ala Arg Gly Gly Leu Pro Leu Leu Thr Pro Met Ser Gln Val Ala Gly 115 120 125 cgc ctc gcc gtg cag gag ggc gcg cac cac ctc atg agc tcc aag ggg 732 Arg Leu Ala Val Gln Glu Gly Ala His His Leu Met Ser Ser Lys Gly 130 135 140 ggc cgc ggc gtg ctg ctc ggt ggc gtg ccc ggc acc gcc ccg gcg aag 780 Gly Arg Gly Val Leu Leu Gly Gly Val Pro Gly Thr Ala Pro Ala Lys 145 150 155 160 gtc gtc gtc ctc ggc ggc ggt cag gtc ggc gcc tcg gcc gtc gcg atc 828 Val Val Val Leu Gly Gly Gly Gln Val Gly Ala Ser Ala Val Ala Ile 165 170 175 gcg cag ggt ctg cgc gcc agc gtc acg gtc ctc gac ctc gac ccg cag 876 Ala Gln Gly Leu Arg Ala Ser Val Thr Val Leu Asp Leu Asp Pro Gln 180 185 190 gtg ctc gcg cgt ttc gac gac ctc tac cag ggc aac gtc cgc acc ctg 924 Val Leu Ala Arg Phe Asp Asp Leu Tyr Gln Gly Asn Val Arg Thr Leu 195 200 205 ctg tcc gac ccg atg acc atc gag acc gag ctt ctc gac gcc gac ctc 972 Leu Ser Asp Pro Met Thr Ile Glu Thr Glu Leu Leu Asp Ala Asp Leu 210 215 220 gtc atc ggc gcc gtc ctc gtc gcc ggc gcg aag gcc ccg acg ctg gtc 1020 Val Ile Gly Ala Val Leu Val Ala Gly Ala Lys Ala Pro Thr Leu Val 225 230 235 240 agc gac gac ctg gtc gcc cgc atg aag cag ggc gcc gtg ctc gtc gac 1068 Ser Asp Asp Leu Val Ala Arg Met Lys Gln Gly Ala Val Leu Val Asp 245 250 255 gtc gcc atc gac cag ggc ggc tgc ttc gag ccc tcc cgc aag acc tcc 1116 Val Ala Ile Asp Gln Gly Gly Cys Phe Glu Pro Ser Arg Lys Thr Ser 260 265 270 cac gag gac ccg acc tac gag gtc cac ggc acc cgc ttc tac tgc gtg 1164 His Glu Asp Pro Thr Tyr Glu Val His Gly Thr Arg Phe Tyr Cys Val 275 280 285 ccc aac atg ccc ggc gcg gtg gcc aac acc tcg acc cgg gcg ctc acc 1212 Pro Asn Met Pro Gly Ala Val Ala Asn Thr Ser Thr Arg Ala Leu Thr 290 295 300 tcg gcg acc ctg ccc tac gtc cgc gcg atc gcg gcg ggc ggc gtg gac 1260 Ser Ala Thr Leu Pro Tyr Val Arg Ala Ile Ala Ala Gly Gly Val Asp 305 310 315 320 cgg gcg gtg gac aac cac ccg ggt ctg gag ccg ggc atc atg acc tgc 1308 Arg Ala Val Asp Asn His Pro Gly Leu Glu Pro Gly Ile Met Thr Cys 325 330 335 gac ggc cgc ctg atc agt gag cca gtg aag gag gcg ttc ccg gat ctt 1356 Asp Gly Arg Leu Ile Ser Glu Pro Val Lys Glu Ala Phe Pro Asp Leu 340 345 350 ctc ggt tga tgagcatcgc cgccacccgc tccatgtgat cccagatctg 1405 Leu Gly gtggcggtac ccggcgggga tggtctcctc gtcgatgctg tcgagtgaca ccttcatcag 1465 ctccagccag cggatcgcag ccgcgcggtc cacggggaac tcgtggtggc ggcggcgcag 1525 catcggggcg ccgcgcttct cgttgaaggt ggccggtccg ccccagtact gggcgaggaa 1585 ccacttgagg cggttctcgg cgccctccca gtcgtcctcg gggtacatgg gccccaggat 1645 gtcgtccgtc ttcacctgcg 1665 <210> 72 <211> 354 <212> PRT <213> Corynebacterium humireducens <400> 72 Met Asn Met Glu Gly Arg Val Ala Leu Ser Pro Leu Gly Ala Gly Thr 1 5 10 15 Leu Val Ala Asp Gly His Glu Val Ile Val Gln Ala Gly Ala Gly Glu 20 25 30 Asn Ser Ser Phe Thr Asp Ala Glu Tyr Glu Ala Thr Gly Ala Arg Val 35 40 45 Val Ala Ser Ala Glu Glu Ala Trp Gly Ala Glu Met Val Leu Lys Val 50 55 60 Lys Glu Pro Leu Pro Glu Glu Tyr Gly Phe Leu Arg Pro Asp Leu Ile 65 70 75 80 Leu Phe Thr Tyr Leu His Leu Ala Ser Ser Arg Glu Cys Thr Lys Ala 85 90 95 Leu Leu Asp Ala Gly Thr Leu Ser Leu Ala Tyr Glu Thr Val Leu Asp 100 105 110 Ala Arg Gly Gly Leu Pro Leu Leu Thr Pro Met Ser Gln Val Ala Gly 115 120 125 Arg Leu Ala Val Gln Glu Gly Ala His His Leu Met Ser Ser Lys Gly 130 135 140 Gly Arg Gly Val Leu Leu Gly Gly Val Pro Gly Thr Ala Pro Ala Lys 145 150 155 160 Val Val Val Leu Gly Gly Gly Gln Val Gly Ala Ser Ala Val Ala Ile 165 170 175 Ala Gln Gly Leu Arg Ala Ser Val Thr Val Leu Asp Leu Asp Pro Gln 180 185 190 Val Leu Ala Arg Phe Asp Asp Leu Tyr Gln Gly Asn Val Arg Thr Leu 195 200 205 Leu Ser Asp Pro Met Thr Ile Glu Thr Glu Leu Leu Asp Ala Asp Leu 210 215 220 Val Ile Gly Ala Val Leu Val Ala Gly Ala Lys Ala Pro Thr Leu Val 225 230 235 240 Ser Asp Asp Leu Val Ala Arg Met Lys Gln Gly Ala Val Leu Val Asp 245 250 255 Val Ala Ile Asp Gln Gly Gly Cys Phe Glu Pro Ser Arg Lys Thr Ser 260 265 270 His Glu Asp Pro Thr Tyr Glu Val His Gly Thr Arg Phe Tyr Cys Val 275 280 285 Pro Asn Met Pro Gly Ala Val Ala Asn Thr Ser Thr Arg Ala Leu Thr 290 295 300 Ser Ala Thr Leu Pro Tyr Val Arg Ala Ile Ala Ala Gly Gly Val Asp 305 310 315 320 Arg Ala Val Asp Asn His Pro Gly Leu Glu Pro Gly Ile Met Thr Cys 325 330 335 Asp Gly Arg Leu Ile Ser Glu Pro Val Lys Glu Ala Phe Pro Asp Leu 340 345 350 Leu Gly <210> 73 <211> 1515 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1215) <223> gene cysD <400> 73 cctgaccccg tacgcggggt gggtcaccgg cctgcgccgc gctgacggac cgacccgggc 60 gcacgccccg gcgctcagcc ttgacgcgac ggggcggctg aagatctcgc cgctggtcac 120 gtggtccctc gtggacacgg aggcgttcat cgcggaccac gacctcatcc gacaccccct 180 gaccacgcag ggctacccgt ccatcgggtg cgccacctgc accctccccg tcgccgacgg 240 ggaggatccc cgggccggcc gctgggccgg acagaacaag acagaatgcg ggctccactc 300 atg acc acc acc acc ctc acc aga ctc tcc ccc cat ctg cag gac ctc 348 Met Thr Thr Thr Thr Leu Thr Arg Leu Ser Pro His Leu Gln Asp Leu 1 5 10 15 gag aac gag tcc atc cac atc ctc cgg gag gtg gcc ggg cag ttc gac 396 Glu Asn Glu Ser Ile His Ile Leu Arg Glu Val Ala Gly Gln Phe Asp 20 25 30 cgg gtc ggg ctg ctg ttc tcc ggc ggc aag gac tcc gtc gtc gtg ctc 444 Arg Val Gly Leu Leu Phe Ser Gly Gly Lys Asp Ser Val Val Val Leu 35 40 45 gag ctg gcg cgc cgg gcc ttc gct ccg gcg gcc gtg ccc ttc gag ctg 492 Glu Leu Ala Arg Arg Ala Phe Ala Pro Ala Ala Val Pro Phe Glu Leu 50 55 60 ctc cac gtg gac acc ggc cac aac ttc ccc gag gtc atc gag ttc cgt 540 Leu His Val Asp Thr Gly His Asn Phe Pro Glu Val Ile Glu Phe Arg 65 70 75 80 gac cga gtc gtc gcc gag acc ggc gtg cgg ctg cgc gtc gcg cac gtg 588 Asp Arg Val Val Ala Glu Thr Gly Val Arg Leu Arg Val Ala His Val 85 90 95 cag gac tgg atc gac cgc ggt gac ctc gtc gaa cgt ccc gac ggc acc 636 Gln Asp Trp Ile Asp Arg Gly Asp Leu Val Glu Arg Pro Asp Gly Thr 100 105 110 cgc aat ccc ctg cag acc gtc ccc ctc gtg gag acc atc gcc gag cag 684 Arg Asn Pro Leu Gln Thr Val Pro Leu Val Glu Thr Ile Ala Glu Gln 115 120 125 ggc tac gac gcc gtc ctc ggt ggc gcc cgc cgc gac gag gag cgg gcg 732 Gly Tyr Asp Ala Val Leu Gly Gly Ala Arg Arg Asp Glu Glu Arg Ala 130 135 140 cgc gcc aag gag cgt gtc ttc tcc gtg cgc gac tcc ttc ggc ggc tgg 780 Arg Ala Lys Glu Arg Val Phe Ser Val Arg Asp Ser Phe Gly Gly Trp 145 150 155 160 gat ccg cgc cgc cag cgc ccg gag ctg tgg cac ctc tac aac ggc ggc 828 Asp Pro Arg Arg Gln Arg Pro Glu Leu Trp His Leu Tyr Asn Gly Gly 165 170 175 cac ctg ccg ggc gag aac gtc cgc gtc ttc ccc atc tcc aac tgg acc 876 His Leu Pro Gly Glu Asn Val Arg Val Phe Pro Ile Ser Asn Trp Thr 180 185 190 gag gcc gac atc tgg gag tac atc ggc gcc cgc ggc atc gcg ctg ccg 924 Glu Ala Asp Ile Trp Glu Tyr Ile Gly Ala Arg Gly Ile Ala Leu Pro 195 200 205 gag atc tac ttc gcg cat gag cgg gcc gtc ttc gag cgg gac ggc atg 972 Glu Ile Tyr Phe Ala His Glu Arg Ala Val Phe Glu Arg Asp Gly Met 210 215 220 tgg ctg acc gcc ggc gag tgg ggt ggg ccc cgc gag ggc gag acc ctc 1020 Trp Leu Thr Ala Gly Glu Trp Gly Gly Pro Arg Glu Gly Glu Thr Leu 225 230 235 240 gag gtc cgc cgc gtg cgc tac cgc acc gtc ggc gac atg tcc tgc acc 1068 Glu Val Arg Arg Val Arg Tyr Arg Thr Val Gly Asp Met Ser Cys Thr 245 250 255 ggt gcg gtg ctc tcc gac gcc acc acg gtc gac gac gtc atc gcc gag 1116 Gly Ala Val Leu Ser Asp Ala Thr Thr Val Asp Asp Val Ile Ala Glu 260 265 270 atc gcc acc tcc acc ctc acc gag cgc ggg gcc acc cgc gcc gac gac 1164 Ile Ala Thr Ser Thr Leu Thr Glu Arg Gly Ala Thr Arg Ala Asp Asp 275 280 285 cgc ctc agt gaa tcc gcc atg gaa gac cgc aag aag gaa ggc tac ttc 1212 Arg Leu Ser Glu Ser Ala Met Glu Asp Arg Lys Lys Glu Gly Tyr Phe 290 295 300 tga tgaccaccac gctcctgcgc acccgcgaga cgctgcgcct gtgcaccgcc 1265 ggctccgtcg acgacggcaa gtccaccctc gtgggccgcc tgctccacga cacccgctcc 1325 gtcctcgccg accagctcgc cgccgtcgag cgcacctccg ccgaccgcgg tttccacggc 1385 ggcctggacc tctcactgct cgtcgacggc ctccgcgccg aacgcgagca gggcatcacc 1445 atcgacgtgg cctaccgcta cttcgccacc gaccgccgca ccttcatcct cgccgacacc 1505 cccggccacg 1515 <210> 74 <211> 304 <212> PRT <213> Corynebacterium humireducens <400> 74 Met Thr Thr Thr Thr Leu Thr Arg Leu Ser Pro His Leu Gln Asp Leu 1 5 10 15 Glu Asn Glu Ser Ile His Ile Leu Arg Glu Val Ala Gly Gln Phe Asp 20 25 30 Arg Val Gly Leu Leu Phe Ser Gly Gly Lys Asp Ser Val Val Val Leu 35 40 45 Glu Leu Ala Arg Arg Ala Phe Ala Pro Ala Ala Val Pro Phe Glu Leu 50 55 60 Leu His Val Asp Thr Gly His Asn Phe Pro Glu Val Ile Glu Phe Arg 65 70 75 80 Asp Arg Val Val Ala Glu Thr Gly Val Arg Leu Arg Val Ala His Val 85 90 95 Gln Asp Trp Ile Asp Arg Gly Asp Leu Val Glu Arg Pro Asp Gly Thr 100 105 110 Arg Asn Pro Leu Gln Thr Val Pro Leu Val Glu Thr Ile Ala Glu Gln 115 120 125 Gly Tyr Asp Ala Val Leu Gly Gly Ala Arg Arg Asp Glu Glu Arg Ala 130 135 140 Arg Ala Lys Glu Arg Val Phe Ser Val Arg Asp Ser Phe Gly Gly Trp 145 150 155 160 Asp Pro Arg Arg Gln Arg Pro Glu Leu Trp His Leu Tyr Asn Gly Gly 165 170 175 His Leu Pro Gly Glu Asn Val Arg Val Phe Pro Ile Ser Asn Trp Thr 180 185 190 Glu Ala Asp Ile Trp Glu Tyr Ile Gly Ala Arg Gly Ile Ala Leu Pro 195 200 205 Glu Ile Tyr Phe Ala His Glu Arg Ala Val Phe Glu Arg Asp Gly Met 210 215 220 Trp Leu Thr Ala Gly Glu Trp Gly Gly Pro Arg Glu Gly Glu Thr Leu 225 230 235 240 Glu Val Arg Arg Val Arg Tyr Arg Thr Val Gly Asp Met Ser Cys Thr 245 250 255 Gly Ala Val Leu Ser Asp Ala Thr Thr Val Asp Asp Val Ile Ala Glu 260 265 270 Ile Ala Thr Ser Thr Leu Thr Glu Arg Gly Ala Thr Arg Ala Asp Asp 275 280 285 Arg Leu Ser Glu Ser Ala Met Glu Asp Arg Lys Lys Glu Gly Tyr Phe 290 295 300 <210> 75 <211> 1575 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1275) <223> gene cysH <400> 75 cgtgcgcatc gccgtcaacg gctgccccaa ctcgtgtgcc cgcacgcagg tcgcggacat 60 cggcttcaag ggccagaccg tcaccgacgc cgacggcaac cgggtcgagg gcttccaggt 120 gcacctcggc gggtcgatga gcctcaacgc caacttcggc cgcaagctcc gcggccacaa 180 ggtgctcgcc gacgacatcg gggactacgt cacccgcgtg gtcacccact tccgggacca 240 gcgccgaccg gaggagacct tccaggagtg ggtccagcgc gccgccgagg aggacctcca 300 gtg agt ttc cgt cgc gcc ccc aac ccg aac cgc aac cat ccg ctg cac 348 Val Ser Phe Arg Arg Ala Pro Asn Pro Asn Arg Asn His Pro Leu His 1 5 10 15 tgc ccg tgg tgc gcc ggc gag gat ctg ctg ccc aac acg gag gac gac 396 Cys Pro Trp Cys Ala Gly Glu Asp Leu Leu Pro Asn Thr Glu Asp Asp 20 25 30 ttc gcc tgg ctg tgc cgc gac tgc acc cgg gtg ttc tcc gtc cgc tac 444 Phe Ala Trp Leu Cys Arg Asp Cys Thr Arg Val Phe Ser Val Arg Tyr 35 40 45 cac ggg cag gac gcc ccg gcg cgc cga ccg gcc ccg gca ctc tcc acg 492 His Gly Gln Asp Ala Pro Ala Arg Arg Pro Ala Pro Ala Leu Ser Thr 50 55 60 agt gcg gcg atc cag ctc tcc ctg tcc cgt cgc ggc cac ctc aac gac 540 Ser Ala Ala Ile Gln Leu Ser Leu Ser Arg Arg Gly His Leu Asn Asp 65 70 75 80 ccc gcc gtc agc ccg gca ggc ccc acc gcc acc ggg gac ctg ccg ggg 588 Pro Ala Val Ser Pro Ala Gly Pro Thr Ala Thr Gly Asp Leu Pro Gly 85 90 95 gcg gtg cgg gag gag aac gcg cgg ctc gtc gag aag cac gcg gat gag 636 Ala Val Arg Glu Glu Asn Ala Arg Leu Val Glu Lys His Ala Asp Glu 100 105 110 ctg tac gac gcc ccc gcc gac gtc atc ctg gcg tgg gcc cgg gag cac 684 Leu Tyr Asp Ala Pro Ala Asp Val Ile Leu Ala Trp Ala Arg Glu His 115 120 125 gtg ccg ggt cgc ctc gcg gtg acg ctc tcg atg gag aac acc gtc ctc 732 Val Pro Gly Arg Leu Ala Val Thr Leu Ser Met Glu Asn Thr Val Leu 130 135 140 gcg gag ctg gcc gcc cgg cac acg ccc gac gcc gac ctg ctc ttc ctc 780 Ala Glu Leu Ala Ala Arg His Thr Pro Asp Ala Asp Leu Leu Phe Leu 145 150 155 160 gac acc ggc tac cac ttc gag gag acg ctg gcg gtg gcg cgg gac gtc 828 Asp Thr Gly Tyr His Phe Glu Glu Thr Leu Ala Val Ala Arg Asp Val 165 170 175 gac agg cgg tac ccg cag acg ctg gtg acc gcg acg ccg acg ctc acc 876 Asp Arg Arg Tyr Pro Gln Thr Leu Val Thr Ala Thr Pro Thr Leu Thr 180 185 190 cgg gcg gag cag gac gcc gcc tac ggg ccc ggc ctg tac cgg cgc gac 924 Arg Ala Glu Gln Asp Ala Ala Tyr Gly Pro Gly Leu Tyr Arg Arg Asp 195 200 205 ccg gcc gcg tgc tgc cgc atg cgc aag gtc gag ccg ctg gcc gtg cac 972 Pro Ala Ala Cys Cys Arg Met Arg Lys Val Glu Pro Leu Ala Val His 210 215 220 ctg acc ccg tac gcg ggg tgg gtc acc ggc ctg cgc cgc gct gac gga 1020 Leu Thr Pro Tyr Ala Gly Trp Val Thr Gly Leu Arg Arg Ala Asp Gly 225 230 235 240 ccg acc cgg gcg cac gcc ccg gcg ctc agc ctt gac gcg acg ggg cgg 1068 Pro Thr Arg Ala His Ala Pro Ala Leu Ser Leu Asp Ala Thr Gly Arg 245 250 255 ctg aag atc tcg ccg ctg gtc acg tgg tcc ctc gtg gac acg gag gcg 1116 Leu Lys Ile Ser Pro Leu Val Thr Trp Ser Leu Val Asp Thr Glu Ala 260 265 270 ttc atc gcg gac cac gac ctc atc cga cac ccc ctg acc acg cag ggc 1164 Phe Ile Ala Asp His Asp Leu Ile Arg His Pro Leu Thr Thr Gln Gly 275 280 285 tac ccg tcc atc ggg tgc gcc acc tgc acc ctc ccc gtc gcc gac ggg 1212 Tyr Pro Ser Ile Gly Cys Ala Thr Cys Thr Leu Pro Val Ala Asp Gly 290 295 300 gag gat ccc cgg gcc ggc cgc tgg gcc gga cag aac aag aca gaa tgc 1260 Glu Asp Pro Arg Ala Gly Arg Trp Ala Gly Gln Asn Lys Thr Glu Cys 305 310 315 320 ggg ctc cac tca tga ccaccaccac cctcaccaga ctctcccccc atctgcagga 1315 Gly Leu His Ser cctcgagaac gagtccatcc acatcctccg ggaggtggcc gggcagttcg accgggtcgg 1375 gctgctgttc tccggcggca aggactccgt cgtcgtgctc gagctggcgc gccgggcctt 1435 cgctccggcg gccgtgccct tcgagctgct ccacgtggac accggccaca acttccccga 1495 ggtcatcgag ttccgtgacc gagtcgtcgc cgagaccggc gtgcggctgc gcgtcgcgca 1555 cgtgcaggac tggatcgacc 1575 <210> 76 <211> 324 <212> PRT <213> Corynebacterium humireducens <400> 76 Val Ser Phe Arg Arg Ala Pro Asn Pro Asn Arg Asn His Pro Leu His 1 5 10 15 Cys Pro Trp Cys Ala Gly Glu Asp Leu Leu Pro Asn Thr Glu Asp Asp 20 25 30 Phe Ala Trp Leu Cys Arg Asp Cys Thr Arg Val Phe Ser Val Arg Tyr 35 40 45 His Gly Gln Asp Ala Pro Ala Arg Arg Pro Ala Pro Ala Leu Ser Thr 50 55 60 Ser Ala Ala Ile Gln Leu Ser Leu Ser Arg Arg Gly His Leu Asn Asp 65 70 75 80 Pro Ala Val Ser Pro Ala Gly Pro Thr Ala Thr Gly Asp Leu Pro Gly 85 90 95 Ala Val Arg Glu Glu Asn Ala Arg Leu Val Glu Lys His Ala Asp Glu 100 105 110 Leu Tyr Asp Ala Pro Ala Asp Val Ile Leu Ala Trp Ala Arg Glu His 115 120 125 Val Pro Gly Arg Leu Ala Val Thr Leu Ser Met Glu Asn Thr Val Leu 130 135 140 Ala Glu Leu Ala Ala Arg His Thr Pro Asp Ala Asp Leu Leu Phe Leu 145 150 155 160 Asp Thr Gly Tyr His Phe Glu Glu Thr Leu Ala Val Ala Arg Asp Val 165 170 175 Asp Arg Arg Tyr Pro Gln Thr Leu Val Thr Ala Thr Pro Thr Leu Thr 180 185 190 Arg Ala Glu Gln Asp Ala Ala Tyr Gly Pro Gly Leu Tyr Arg Arg Asp 195 200 205 Pro Ala Ala Cys Cys Arg Met Arg Lys Val Glu Pro Leu Ala Val His 210 215 220 Leu Thr Pro Tyr Ala Gly Trp Val Thr Gly Leu Arg Arg Ala Asp Gly 225 230 235 240 Pro Thr Arg Ala His Ala Pro Ala Leu Ser Leu Asp Ala Thr Gly Arg 245 250 255 Leu Lys Ile Ser Pro Leu Val Thr Trp Ser Leu Val Asp Thr Glu Ala 260 265 270 Phe Ile Ala Asp His Asp Leu Ile Arg His Pro Leu Thr Thr Gln Gly 275 280 285 Tyr Pro Ser Ile Gly Cys Ala Thr Cys Thr Leu Pro Val Ala Asp Gly 290 295 300 Glu Asp Pro Arg Ala Gly Arg Trp Ala Gly Gln Asn Lys Thr Glu Cys 305 310 315 320 Gly Leu His Ser <210> 77 <211> 2295 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1995) <223> gene cysI <400> 77 gaaagtagca cccctgggcg gtaatgcaag cgaaaaggcg acttcaattc agggtccggg 60 catcgctctt cgacgccccc aattcaattc agtgaatgta tgtcggatat aaacactacg 120 tacagccatg atcgggaaag ttgcccaaaa tcatgacgta tctacatctc tccaccattg 180 ccatgaactg atctgtctgc tctaatccac tgcagacgcc ccggcagggg ctccgtggcg 240 gcgcccggcg ccgaatccac gtgaatgaaa tagacagaac agttcagtag ggagatggtc 300 atg gac acg acc acc acg atc gag aag gca ccc cgc cgc gag cgc ccc 348 Met Asp Thr Thr Thr Thr Ile Glu Lys Ala Pro Arg Arg Glu Arg Pro 1 5 10 15 aga cgc gcc cgc aag ccg gag ggc cag tgg cgg atc gac ggc acc acg 396 Arg Arg Ala Arg Lys Pro Glu Gly Gln Trp Arg Ile Asp Gly Thr Thr 20 25 30 ccg ctc aac cac gtg gag gag gtg aag cag gac ggc gac atc ctc gcc 444 Pro Leu Asn His Val Glu Glu Val Lys Gln Asp Gly Asp Ile Leu Ala 35 40 45 gtc tac gac cgg gtg gtc aac atc tac tcg aag cag ggt ttc gac tcc 492 Val Tyr Asp Arg Val Val Asn Ile Tyr Ser Lys Gln Gly Phe Asp Ser 50 55 60 atc ccc gcc gac gac ctc gcc ccg cgc ttc aag tgg ctg ggc ctg tac 540 Ile Pro Ala Asp Asp Leu Ala Pro Arg Phe Lys Trp Leu Gly Leu Tyr 65 70 75 80 acg cag cgc cgc cag gat ctc ggc ggt gag ctc acc ggc cag ctg ccc 588 Thr Gln Arg Arg Gln Asp Leu Gly Gly Glu Leu Thr Gly Gln Leu Pro 85 90 95 gac gcc gag ctg cag gac cgc tac ttc atg atg cgc gtg cgt ttc gag 636 Asp Ala Glu Leu Gln Asp Arg Tyr Phe Met Met Arg Val Arg Phe Glu 100 105 110 ggc ggc cag gta acc ccg gcc cgc ctc cgc gcg gtg ggg gag atc tcc 684 Gly Gly Gln Val Thr Pro Ala Arg Leu Arg Ala Val Gly Glu Ile Ser 115 120 125 cgc gac tac gcc cgc agc acc gcg gac ttc acc gat cgg cag aac atc 732 Arg Asp Tyr Ala Arg Ser Thr Ala Asp Phe Thr Asp Arg Gln Asn Ile 130 135 140 cag ctg cac tgg ctg cgc atc gag gac atc ccg gcg atc tgg gag aag 780 Gln Leu His Trp Leu Arg Ile Glu Asp Ile Pro Ala Ile Trp Glu Lys 145 150 155 160 ctc gac tcc gtg ggg ctc agc ccc ctg ctg ggc tgc ggt gac gtg ccg 828 Leu Asp Ser Val Gly Leu Ser Pro Leu Leu Gly Cys Gly Asp Val Pro 165 170 175 cgc atc atc ctc ggc tcc ccg gtc gcg ggg gtc gcc gcc gag gag atc 876 Arg Ile Ile Leu Gly Ser Pro Val Ala Gly Val Ala Ala Glu Glu Ile 180 185 190 atc gac gcc acc ccg gcg atc gag cgg atc cag cgc gag cac ctg ccg 924 Ile Asp Ala Thr Pro Ala Ile Glu Arg Ile Gln Arg Glu His Leu Pro 195 200 205 aac ccg gag ttc cac aac ctg ccg cgc aag ttc aag tcc gcg atc tcc 972 Asn Pro Glu Phe His Asn Leu Pro Arg Lys Phe Lys Ser Ala Ile Ser 210 215 220 ggc cac tcg cgc cag gac gtc acc cac gag atc cag gac gtc gcc ttc 1020 Gly His Ser Arg Gln Asp Val Thr His Glu Ile Gln Asp Val Ala Phe 225 230 235 240 atc ggt tcc gtc cac ccc gag cac ggc ccg ggt ttc gag tgc ttc gtc 1068 Ile Gly Ser Val His Pro Glu His Gly Pro Gly Phe Glu Cys Phe Val 245 250 255 ggc ggt ggc ctg tcc acc aac ccg atg ctc gcg cag tcg ctc ggc gcg 1116 Gly Gly Gly Leu Ser Thr Asn Pro Met Leu Ala Gln Ser Leu Gly Ala 260 265 270 tgg atc ccg ctg gag gac gtg ccg gac gtg tgg gcc ggc gtc gcc cgg 1164 Trp Ile Pro Leu Glu Asp Val Pro Asp Val Trp Ala Gly Val Ala Arg 275 280 285 atc ttc cgc gac tac ggt tac cgc cgc ctg cgg aat cgt gcc cgt ctc 1212 Ile Phe Arg Asp Tyr Gly Tyr Arg Arg Leu Arg Asn Arg Ala Arg Leu 290 295 300 aag ttc ctc gtg gcg gaa tgg ggg atc gag aag ttc cgg gac gtc ctc 1260 Lys Phe Leu Val Ala Glu Trp Gly Ile Glu Lys Phe Arg Asp Val Leu 305 310 315 320 gag acg gag tac ctc gga cgc ccg ctc atc gac gga ccc cgc cag ccc 1308 Glu Thr Glu Tyr Leu Gly Arg Pro Leu Ile Asp Gly Pro Arg Gln Pro 325 330 335 acc aac ccc ggc tac cgc gac cac atc ggc atc cac ccg cag aag gac 1356 Thr Asn Pro Gly Tyr Arg Asp His Ile Gly Ile His Pro Gln Lys Asp 340 345 350 ggc cgc ttc tac ctg ggc gtg aag ccg acc gtc ggg cac acc acc ggt 1404 Gly Arg Phe Tyr Leu Gly Val Lys Pro Thr Val Gly His Thr Thr Gly 355 360 365 gag cag ctc atc gcc atc gcc gag gtg gcg gag cgt ttc ggc atc gag 1452 Glu Gln Leu Ile Ala Ile Ala Glu Val Ala Glu Arg Phe Gly Ile Glu 370 375 380 cgc atc cgc acc acc tcg gag aag gag ctg ctc ttc ctc gac gtc gag 1500 Arg Ile Arg Thr Thr Ser Glu Lys Glu Leu Leu Phe Leu Asp Val Glu 385 390 395 400 cgc acc gac ctc acc gcc ctg gcc gcc gcc ctc gac gag acc ggc ctg 1548 Arg Thr Asp Leu Thr Ala Leu Ala Ala Ala Leu Asp Glu Thr Gly Leu 405 410 415 tac tcg aag ccg agc gag tgg cgc cgc aac atc atc tcg tgc acc ggc 1596 Tyr Ser Lys Pro Ser Glu Trp Arg Arg Asn Ile Ile Ser Cys Thr Gly 420 425 430 ctg gag ttc tgc aaa ctg gcg cac gcg acg acc aag tcg cgt gcc gtg 1644 Leu Glu Phe Cys Lys Leu Ala His Ala Thr Thr Lys Ser Arg Ala Val 435 440 445 gcc ctg gtc gac gag ctg gag gaa cgc gtc ggc gac atc gac gtg ccc 1692 Ala Leu Val Asp Glu Leu Glu Glu Arg Val Gly Asp Ile Asp Val Pro 450 455 460 gtg cgc atc gcc gtc aac ggc tgc ccc aac tcg tgt gcc cgc acg cag 1740 Val Arg Ile Ala Val Asn Gly Cys Pro Asn Ser Cys Ala Arg Thr Gln 465 470 475 480 gtc gcg gac atc ggc ttc aag ggc cag acc gtc acc gac gcc gac ggc 1788 Val Ala Asp Ile Gly Phe Lys Gly Gln Thr Val Thr Asp Ala Asp Gly 485 490 495 aac cgg gtc gag ggc ttc cag gtg cac ctc ggc ggg tcg atg agc ctc 1836 Asn Arg Val Glu Gly Phe Gln Val His Leu Gly Gly Ser Met Ser Leu 500 505 510 aac gcc aac ttc ggc cgc aag ctc cgc ggc cac aag gtg ctc gcc gac 1884 Asn Ala Asn Phe Gly Arg Lys Leu Arg Gly His Lys Val Leu Ala Asp 515 520 525 gac atc ggg gac tac gtc acc cgc gtg gtc acc cac ttc cgg gac cag 1932 Asp Ile Gly Asp Tyr Val Thr Arg Val Val Thr His Phe Arg Asp Gln 530 535 540 cgc cga ccg gag gag acc ttc cag gag tgg gtc cag cgc gcc gcc gag 1980 Arg Arg Pro Glu Glu Thr Phe Gln Glu Trp Val Gln Arg Ala Ala Glu 545 550 555 560 gag gac ctc cag tga gtttccgtcg cgcccccaac ccgaaccgca accatccgct 2035 Glu Asp Leu Gln gcactgcccg tggtgcgccg gcgaggatct gctgcccaac acggaggacg acttcgcctg 2095 gctgtgccgc gactgcaccc gggtgttctc cgtccgctac cacgggcagg acgccccggc 2155 gcgccgaccg gccccggcac tctccacgag tgcggcgatc cagctctccc tgtcccgtcg 2215 cggccacctc aacgaccccg ccgtcagccc ggcaggcccc accgccaccg gggacctgcc 2275 gggggcggtg cgggaggaga 2295 <210> 78 <211> 564 <212> PRT <213> Corynebacterium humireducens <400> 78 Met Asp Thr Thr Thr Thr Ile Glu Lys Ala Pro Arg Arg Glu Arg Pro 1 5 10 15 Arg Arg Ala Arg Lys Pro Glu Gly Gln Trp Arg Ile Asp Gly Thr Thr 20 25 30 Pro Leu Asn His Val Glu Glu Val Lys Gln Asp Gly Asp Ile Leu Ala 35 40 45 Val Tyr Asp Arg Val Val Asn Ile Tyr Ser Lys Gln Gly Phe Asp Ser 50 55 60 Ile Pro Ala Asp Asp Leu Ala Pro Arg Phe Lys Trp Leu Gly Leu Tyr 65 70 75 80 Thr Gln Arg Arg Gln Asp Leu Gly Gly Glu Leu Thr Gly Gln Leu Pro 85 90 95 Asp Ala Glu Leu Gln Asp Arg Tyr Phe Met Met Arg Val Arg Phe Glu 100 105 110 Gly Gly Gln Val Thr Pro Ala Arg Leu Arg Ala Val Gly Glu Ile Ser 115 120 125 Arg Asp Tyr Ala Arg Ser Thr Ala Asp Phe Thr Asp Arg Gln Asn Ile 130 135 140 Gln Leu His Trp Leu Arg Ile Glu Asp Ile Pro Ala Ile Trp Glu Lys 145 150 155 160 Leu Asp Ser Val Gly Leu Ser Pro Leu Leu Gly Cys Gly Asp Val Pro 165 170 175 Arg Ile Ile Leu Gly Ser Pro Val Ala Gly Val Ala Ala Glu Glu Ile 180 185 190 Ile Asp Ala Thr Pro Ala Ile Glu Arg Ile Gln Arg Glu His Leu Pro 195 200 205 Asn Pro Glu Phe His Asn Leu Pro Arg Lys Phe Lys Ser Ala Ile Ser 210 215 220 Gly His Ser Arg Gln Asp Val Thr His Glu Ile Gln Asp Val Ala Phe 225 230 235 240 Ile Gly Ser Val His Pro Glu His Gly Pro Gly Phe Glu Cys Phe Val 245 250 255 Gly Gly Gly Leu Ser Thr Asn Pro Met Leu Ala Gln Ser Leu Gly Ala 260 265 270 Trp Ile Pro Leu Glu Asp Val Pro Asp Val Trp Ala Gly Val Ala Arg 275 280 285 Ile Phe Arg Asp Tyr Gly Tyr Arg Arg Leu Arg Asn Arg Ala Arg Leu 290 295 300 Lys Phe Leu Val Ala Glu Trp Gly Ile Glu Lys Phe Arg Asp Val Leu 305 310 315 320 Glu Thr Glu Tyr Leu Gly Arg Pro Leu Ile Asp Gly Pro Arg Gln Pro 325 330 335 Thr Asn Pro Gly Tyr Arg Asp His Ile Gly Ile His Pro Gln Lys Asp 340 345 350 Gly Arg Phe Tyr Leu Gly Val Lys Pro Thr Val Gly His Thr Thr Gly 355 360 365 Glu Gln Leu Ile Ala Ile Ala Glu Val Ala Glu Arg Phe Gly Ile Glu 370 375 380 Arg Ile Arg Thr Thr Ser Glu Lys Glu Leu Leu Phe Leu Asp Val Glu 385 390 395 400 Arg Thr Asp Leu Thr Ala Leu Ala Ala Ala Leu Asp Glu Thr Gly Leu 405 410 415 Tyr Ser Lys Pro Ser Glu Trp Arg Arg Asn Ile Ile Ser Cys Thr Gly 420 425 430 Leu Glu Phe Cys Lys Leu Ala His Ala Thr Thr Lys Ser Arg Ala Val 435 440 445 Ala Leu Val Asp Glu Leu Glu Glu Arg Val Gly Asp Ile Asp Val Pro 450 455 460 Val Arg Ile Ala Val Asn Gly Cys Pro Asn Ser Cys Ala Arg Thr Gln 465 470 475 480 Val Ala Asp Ile Gly Phe Lys Gly Gln Thr Val Thr Asp Ala Asp Gly 485 490 495 Asn Arg Val Glu Gly Phe Gln Val His Leu Gly Gly Ser Met Ser Leu 500 505 510 Asn Ala Asn Phe Gly Arg Lys Leu Arg Gly His Lys Val Leu Ala Asp 515 520 525 Asp Ile Gly Asp Tyr Val Thr Arg Val Val Thr His Phe Arg Asp Gln 530 535 540 Arg Arg Pro Glu Glu Thr Phe Gln Glu Trp Val Gln Arg Ala Ala Glu 545 550 555 560 Glu Asp Leu Gln <210> 79 <211> 1989 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1689) <223> gene cysJ <400> 79 acgtggattc ggcgccgggc gccgccacgg agcccctgcc ggggcgtctg cagtggatta 60 gagcagacag atcagttcat ggcaatggtg gagagatgta gatacgtcat gattttgggc 120 aactttcccg atcatggctg tacgtagtgt ttatatccga catacattca ctgaattgaa 180 ttgggggcgt cgaagagcga tgcccggacc ctgaattgaa gtcgcctttt cgcttgcatt 240 accgcccagg ggtgctactt tcacagacca gtcagtctag tgatttcccg aaaggaaccc 300 atg agc cag ccc acc ctc cgc atc gcc gtc atc ggc gcc ggc ccc gcc 348 Met Ser Gln Pro Thr Leu Arg Ile Ala Val Ile Gly Ala Gly Pro Ala 1 5 10 15 ggc atc tac gcc tcc gac ctc ctg gtg aag aac gcc gcc tcc atc ggc 396 Gly Ile Tyr Ala Ser Asp Leu Leu Val Lys Asn Ala Ala Ser Ile Gly 20 25 30 cgt gag gtc cac gtc gac ctc atc gag aag ctg ccc gcc ccc ttc ggg 444 Arg Glu Val His Val Asp Leu Ile Glu Lys Leu Pro Ala Pro Phe Gly 35 40 45 ctc atc cgc tac ggc gtc gcc ccc gac cac ccg cgc atc aag ggc atc 492 Leu Ile Arg Tyr Gly Val Ala Pro Asp His Pro Arg Ile Lys Gly Ile 50 55 60 atc acc tcc ctg cac cgc gtc ctc gac cgc ccc gag atc cgc ctg ctc 540 Ile Thr Ser Leu His Arg Val Leu Asp Arg Pro Glu Ile Arg Leu Leu 65 70 75 80 ggc aac atc gag atc ggc cgc gac gtc acc gtc gag gag ctg cgc gaa 588 Gly Asn Ile Glu Ile Gly Arg Asp Val Thr Val Glu Glu Leu Arg Glu 85 90 95 cac tac gac gcc gtc atc ttc gcc acc ggc gcc atc gcc gac cgc gac 636 His Tyr Asp Ala Val Ile Phe Ala Thr Gly Ala Ile Ala Asp Arg Asp 100 105 110 ctg gac atc ccg ggc gtc gac gcg cag ggc tcc ttc ggc gcc gcc gac 684 Leu Asp Ile Pro Gly Val Asp Ala Gln Gly Ser Phe Gly Ala Ala Asp 115 120 125 ttc gtc ggc ttc tac gac ggc aac ccc gag ttc ccc cgc acc tgg gac 732 Phe Val Gly Phe Tyr Asp Gly Asn Pro Glu Phe Pro Arg Thr Trp Asp 130 135 140 ctg tcc gcg cag tcc gtc gcc gtc atc ggc gtg ggc aac gtg ggc ctc 780 Leu Ser Ala Gln Ser Val Ala Val Ile Gly Val Gly Asn Val Gly Leu 145 150 155 160 gac gtc gcg cgc gtc ctg tcc aag acc gcc gac gaa ctc aac gtc acc 828 Asp Val Ala Arg Val Leu Ser Lys Thr Ala Asp Glu Leu Asn Val Thr 165 170 175 gag atc ccc gac aac gtc tac gag acc ctc cgc acc aac cag gcc acc 876 Glu Ile Pro Asp Asn Val Tyr Glu Thr Leu Arg Thr Asn Gln Ala Thr 180 185 190 gag gtc cac gtc ttc ggc cgc cgc ggc ccc gcg cag gcc aag ttc acc 924 Glu Val His Val Phe Gly Arg Arg Gly Pro Ala Gln Ala Lys Phe Thr 195 200 205 ccg cag gaa ctc aag gaa ctc gac cac tcc ccc acc gtc aac gtc gtc 972 Pro Gln Glu Leu Lys Glu Leu Asp His Ser Pro Thr Val Asn Val Val 210 215 220 gtc gac ccc gag gac atc gac tac gac gac gcc agc atc gag gcc cgc 1020 Val Asp Pro Glu Asp Ile Asp Tyr Asp Asp Ala Ser Ile Glu Ala Arg 225 230 235 240 agc agc tcc aag tcg cag gac ctc gtg tgc cag acc ctc gag ggc tac 1068 Ser Ser Ser Lys Ser Gln Asp Leu Val Cys Gln Thr Leu Glu Gly Tyr 245 250 255 gcc atc cgc gaa ccc aag gac gcc ccg cac acc ctg cag atc cac ctc 1116 Ala Ile Arg Glu Pro Lys Asp Ala Pro His Thr Leu Gln Ile His Leu 260 265 270 ttc gag tct ccc acc gag atc ctc acc cgc gac ggc cgt gtc gtc ggc 1164 Phe Glu Ser Pro Thr Glu Ile Leu Thr Arg Asp Gly Arg Val Val Gly 275 280 285 ctg cgc acc gaa cgc acc gaa ctc gac ggc acc ggc cac gtc cgc ggc 1212 Leu Arg Thr Glu Arg Thr Glu Leu Asp Gly Thr Gly His Val Arg Gly 290 295 300 acc ggc acc ttc acc gac tgg ccc gtc cag gcc gtc tac cgc gcc gtc 1260 Thr Gly Thr Phe Thr Asp Trp Pro Val Gln Ala Val Tyr Arg Ala Val 305 310 315 320 ggc tac cgc tcc gag ccg gtg ccg ggc gtg ccc ttc aac cat gtc gag 1308 Gly Tyr Arg Ser Glu Pro Val Pro Gly Val Pro Phe Asn His Val Glu 325 330 335 cat gtg atc ccg aac gac ggc ggc cac gtc ctc gac ccc gcc ggc acc 1356 His Val Ile Pro Asn Asp Gly Gly His Val Leu Asp Pro Ala Gly Thr 340 345 350 ccc gtc ccc ggc ctc tac gcc acc ggc tgg atc aag cgc ggc ccc gtc 1404 Pro Val Pro Gly Leu Tyr Ala Thr Gly Trp Ile Lys Arg Gly Pro Val 355 360 365 gga ctc atc ggc aac acc aag tcc gac gcc aag gag acc acc gac ctc 1452 Gly Leu Ile Gly Asn Thr Lys Ser Asp Ala Lys Glu Thr Thr Asp Leu 370 375 380 ctg ctc gcc gac gcc gcc gcc gga ctt ctc gac gcc ccc ctc cac ccc 1500 Leu Leu Ala Asp Ala Ala Ala Gly Leu Leu Asp Ala Pro Leu His Pro 385 390 395 400 gcc gac gcc ttc ctc cag ctt ctc gac gcc cgc ggc atc ccc gtc acc 1548 Ala Asp Ala Phe Leu Gln Leu Leu Asp Ala Arg Gly Ile Pro Val Thr 405 410 415 acc tgg gag ggc tgg tac cgg ctc gac gcc gcc gag cgc gcc ctc ggc 1596 Thr Trp Glu Gly Trp Tyr Arg Leu Asp Ala Ala Glu Arg Ala Leu Gly 420 425 430 gag gcc tcc ggc gtg gag ggc aag gag cgc cgg aag atc gtc gaa tgg 1644 Glu Ala Ser Gly Val Glu Gly Lys Glu Arg Arg Lys Ile Val Glu Trp 435 440 445 gag gag atg gtc agc cac gca cac atc acc ccg gcc tat gtg tga 1689 Glu Glu Met Val Ser His Ala His Ile Thr Pro Ala Tyr Val 450 455 460 tttctcaggc tcagggccgg cagctgagac tgggcagcgc tatgccacgg gccatgacac 1749 gccgggccgg ataaaaattc ctgaccctga gtcgatctaa ctgcggtaac ggtccggtag 1809 gcgaattgtt accgatcagc aaccgtccaa attcataccc actaatgtga tcactgttac 1869 tctcaagagt ggaaagtaca actgacaatt cttaaggaga aacatcatgc gcggtagcgg 1929 actcatctgg accatcgtcg gcatcctggc catcatcgcc ctagtcatct ggatcatgtc 1989 <210> 80 <211> 462 <212> PRT <213> Corynebacterium humireducens <400> 80 Met Ser Gln Pro Thr Leu Arg Ile Ala Val Ile Gly Ala Gly Pro Ala 1 5 10 15 Gly Ile Tyr Ala Ser Asp Leu Leu Val Lys Asn Ala Ala Ser Ile Gly 20 25 30 Arg Glu Val His Val Asp Leu Ile Glu Lys Leu Pro Ala Pro Phe Gly 35 40 45 Leu Ile Arg Tyr Gly Val Ala Pro Asp His Pro Arg Ile Lys Gly Ile 50 55 60 Ile Thr Ser Leu His Arg Val Leu Asp Arg Pro Glu Ile Arg Leu Leu 65 70 75 80 Gly Asn Ile Glu Ile Gly Arg Asp Val Thr Val Glu Glu Leu Arg Glu 85 90 95 His Tyr Asp Ala Val Ile Phe Ala Thr Gly Ala Ile Ala Asp Arg Asp 100 105 110 Leu Asp Ile Pro Gly Val Asp Ala Gln Gly Ser Phe Gly Ala Ala Asp 115 120 125 Phe Val Gly Phe Tyr Asp Gly Asn Pro Glu Phe Pro Arg Thr Trp Asp 130 135 140 Leu Ser Ala Gln Ser Val Ala Val Ile Gly Val Gly Asn Val Gly Leu 145 150 155 160 Asp Val Ala Arg Val Leu Ser Lys Thr Ala Asp Glu Leu Asn Val Thr 165 170 175 Glu Ile Pro Asp Asn Val Tyr Glu Thr Leu Arg Thr Asn Gln Ala Thr 180 185 190 Glu Val His Val Phe Gly Arg Arg Gly Pro Ala Gln Ala Lys Phe Thr 195 200 205 Pro Gln Glu Leu Lys Glu Leu Asp His Ser Pro Thr Val Asn Val Val 210 215 220 Val Asp Pro Glu Asp Ile Asp Tyr Asp Asp Ala Ser Ile Glu Ala Arg 225 230 235 240 Ser Ser Ser Lys Ser Gln Asp Leu Val Cys Gln Thr Leu Glu Gly Tyr 245 250 255 Ala Ile Arg Glu Pro Lys Asp Ala Pro His Thr Leu Gln Ile His Leu 260 265 270 Phe Glu Ser Pro Thr Glu Ile Leu Thr Arg Asp Gly Arg Val Val Gly 275 280 285 Leu Arg Thr Glu Arg Thr Glu Leu Asp Gly Thr Gly His Val Arg Gly 290 295 300 Thr Gly Thr Phe Thr Asp Trp Pro Val Gln Ala Val Tyr Arg Ala Val 305 310 315 320 Gly Tyr Arg Ser Glu Pro Val Pro Gly Val Pro Phe Asn His Val Glu 325 330 335 His Val Ile Pro Asn Asp Gly Gly His Val Leu Asp Pro Ala Gly Thr 340 345 350 Pro Val Pro Gly Leu Tyr Ala Thr Gly Trp Ile Lys Arg Gly Pro Val 355 360 365 Gly Leu Ile Gly Asn Thr Lys Ser Asp Ala Lys Glu Thr Thr Asp Leu 370 375 380 Leu Leu Ala Asp Ala Ala Ala Gly Leu Leu Asp Ala Pro Leu His Pro 385 390 395 400 Ala Asp Ala Phe Leu Gln Leu Leu Asp Ala Arg Gly Ile Pro Val Thr 405 410 415 Thr Trp Glu Gly Trp Tyr Arg Leu Asp Ala Ala Glu Arg Ala Leu Gly 420 425 430 Glu Ala Ser Gly Val Glu Gly Lys Glu Arg Arg Lys Ile Val Glu Trp 435 440 445 Glu Glu Met Val Ser His Ala His Ile Thr Pro Ala Tyr Val 450 455 460 <210> 81 <211> 1869 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1569) <223> gene cysN <400> 81 cgcgctgccg gagatctact tcgcgcatga gcgggccgtc ttcgagcggg acggcatgtg 60 gctgaccgcc ggcgagtggg gtgggccccg cgagggcgag accctcgagg tccgccgcgt 120 gcgctaccgc accgtcggcg acatgtcctg caccggtgcg gtgctctccg acgccaccac 180 ggtcgacgac gtcatcgccg agatcgccac ctccaccctc accgagcgcg gggccacccg 240 cgccgacgac cgcctcagtg aatccgccat ggaagaccgc aagaaggaag gctacttctg 300 atg acc acc acg ctc ctg cgc acc cgc gag acg ctg cgc ctg tgc acc 348 Met Thr Thr Thr Leu Leu Arg Thr Arg Glu Thr Leu Arg Leu Cys Thr 1 5 10 15 gcc ggc tcc gtc gac gac ggc aag tcc acc ctc gtg ggc cgc ctg ctc 396 Ala Gly Ser Val Asp Asp Gly Lys Ser Thr Leu Val Gly Arg Leu Leu 20 25 30 cac gac acc cgc tcc gtc ctc gcc gac cag ctc gcc gcc gtc gag cgc 444 His Asp Thr Arg Ser Val Leu Ala Asp Gln Leu Ala Ala Val Glu Arg 35 40 45 acc tcc gcc gac cgc ggt ttc cac ggc ggc ctg gac ctc tca ctg ctc 492 Thr Ser Ala Asp Arg Gly Phe His Gly Gly Leu Asp Leu Ser Leu Leu 50 55 60 gtc gac ggc ctc cgc gcc gaa cgc gag cag ggc atc acc atc gac gtg 540 Val Asp Gly Leu Arg Ala Glu Arg Glu Gln Gly Ile Thr Ile Asp Val 65 70 75 80 gcc tac cgc tac ttc gcc acc gac cgc cgc acc ttc atc ctc gcc gac 588 Ala Tyr Arg Tyr Phe Ala Thr Asp Arg Arg Thr Phe Ile Leu Ala Asp 85 90 95 acc ccc ggc cac gtg cag tac acg cgc aac acc gtc acc ggc gtg tcc 636 Thr Pro Gly His Val Gln Tyr Thr Arg Asn Thr Val Thr Gly Val Ser 100 105 110 acg tcg cag gtg gtc gtc ctg ctt gtc gac gcc cgc cac ggc gtc gtc 684 Thr Ser Gln Val Val Val Leu Leu Val Asp Ala Arg His Gly Val Val 115 120 125 gag cag acc cgc cgc cac ctc acc gtc gcg gcg ctc ctc ggg gtg cgc 732 Glu Gln Thr Arg Arg His Leu Thr Val Ala Ala Leu Leu Gly Val Arg 130 135 140 aca gtg ctc ctc gcg gtg aat aag atc gac ctc gtt gac tgg tcc gag 780 Thr Val Leu Leu Ala Val Asn Lys Ile Asp Leu Val Asp Trp Ser Glu 145 150 155 160 gcc gtc ttc cgg gac atc gag gcg gag ttc acg tcg ctg gcc acc gac 828 Ala Val Phe Arg Asp Ile Glu Ala Glu Phe Thr Ser Leu Ala Thr Asp 165 170 175 gtc ggc gtc gac gac gcg cac gtc atc ccc gtc tcc gcg ctg ctg ggc 876 Val Gly Val Asp Asp Ala His Val Ile Pro Val Ser Ala Leu Leu Gly 180 185 190 gac aac gtc gtc gaa ccc tcg gcg aac ctc ccc tgg tac gag ggg ccg 924 Asp Asn Val Val Glu Pro Ser Ala Asn Leu Pro Trp Tyr Glu Gly Pro 195 200 205 acg gtg ctg gaa ctg ctc gag agc gtc gag gtc cac cgc ggc cgc gcc 972 Thr Val Leu Glu Leu Leu Glu Ser Val Glu Val His Arg Gly Arg Ala 210 215 220 cac gcc ctc ggc ttc cgc ctg ccg atc cag tac gtc atc cgc gag cac 1020 His Ala Leu Gly Phe Arg Leu Pro Ile Gln Tyr Val Ile Arg Glu His 225 230 235 240 tcc tcc gac tac cgc ggc tac gcc ggg cgt gtc gac gcc ggc gtg ctc 1068 Ser Ser Asp Tyr Arg Gly Tyr Ala Gly Arg Val Asp Ala Gly Val Leu 245 250 255 acc gtc ggc gac acc gtc cac ctc ccc gag ggg cgc acc agc acc gtc 1116 Thr Val Gly Asp Thr Val His Leu Pro Glu Gly Arg Thr Ser Thr Val 260 265 270 acc cac atc gac acc gcc gac ggc gag gtc cgc tcc gcc cac gcc ggt 1164 Thr His Ile Asp Thr Ala Asp Gly Glu Val Arg Ser Ala His Ala Gly 275 280 285 gac tcc gtg gtc ctg cgc ctg gcc gac gac atc gac ctc atc cgc ggc 1212 Asp Ser Val Val Leu Arg Leu Ala Asp Asp Ile Asp Leu Ile Arg Gly 290 295 300 gac ctc atc gcc ggt gag gac cgc ccg gag ccg acc cgc tcc ttc gcc 1260 Asp Leu Ile Ala Gly Glu Asp Arg Pro Glu Pro Thr Arg Ser Phe Ala 305 310 315 320 gcc aca ctc gtc gga ctc ggc gac cgc acc ctg cgc ccc ggc tcc ccc 1308 Ala Thr Leu Val Gly Leu Gly Asp Arg Thr Leu Arg Pro Gly Ser Pro 325 330 335 gtg aag gtc cgc tac ggc acc tca ctc gtg cgg gga cgc gtc gcg gcg 1356 Val Lys Val Arg Tyr Gly Thr Ser Leu Val Arg Gly Arg Val Ala Ala 340 345 350 atc gag cgg ctg ctc gac atc gac ggc cgc acc gac cac ctc tcc ccg 1404 Ile Glu Arg Leu Leu Asp Ile Asp Gly Arg Thr Asp His Leu Ser Pro 355 360 365 gac gac ctc ggc ctc aac gac atc gcc cac gtg cgt atc gac gtc gcc 1452 Asp Asp Leu Gly Leu Asn Asp Ile Ala His Val Arg Ile Asp Val Ala 370 375 380 acc gag ctc ccc gtc gag ccc tac gcc gcc cgc ggc gcc gtc ggc tcc 1500 Thr Glu Leu Pro Val Glu Pro Tyr Ala Ala Arg Gly Ala Val Gly Ser 385 390 395 400 ttc ctg ctc atc gac cag gcc agc ggc gac acc ctc gcc gcc ggg ctc 1548 Phe Leu Leu Ile Asp Gln Ala Ser Gly Asp Thr Leu Ala Ala Gly Leu 405 410 415 gtc ggc cac cgg ctc cgc tga cccgtgaccg ccctgctcac cctctcccac 1599 Val Gly His Arg Leu Arg 420 gggtcccgcc acccgcgcgc cgccgccggc gtcgaccgcc tcacccgggc ggccggggag 1659 gtgctgggcg tcaccgcccg cgccgcccac ctcgagttcg acaccccctc cctcatcgac 1719 gccgcccgcg acctcgtcgc ccgcggccac gaccacgccg tggtcgtccc cctgctgttc 1779 accgacgcct tccacgcgcg tcacgacgtc cccgccgccc tcgacgcggc ccgccgcgcc 1839 accggcctgc ggctcacgcc cgcccggggc 1869 <210> 82 <211> 422 <212> PRT <213> Corynebacterium humireducens <400> 82 Met Thr Thr Thr Leu Leu Arg Thr Arg Glu Thr Leu Arg Leu Cys Thr 1 5 10 15 Ala Gly Ser Val Asp Asp Gly Lys Ser Thr Leu Val Gly Arg Leu Leu 20 25 30 His Asp Thr Arg Ser Val Leu Ala Asp Gln Leu Ala Ala Val Glu Arg 35 40 45 Thr Ser Ala Asp Arg Gly Phe His Gly Gly Leu Asp Leu Ser Leu Leu 50 55 60 Val Asp Gly Leu Arg Ala Glu Arg Glu Gln Gly Ile Thr Ile Asp Val 65 70 75 80 Ala Tyr Arg Tyr Phe Ala Thr Asp Arg Arg Thr Phe Ile Leu Ala Asp 85 90 95 Thr Pro Gly His Val Gln Tyr Thr Arg Asn Thr Val Thr Gly Val Ser 100 105 110 Thr Ser Gln Val Val Val Leu Leu Val Asp Ala Arg His Gly Val Val 115 120 125 Glu Gln Thr Arg Arg His Leu Thr Val Ala Ala Leu Leu Gly Val Arg 130 135 140 Thr Val Leu Leu Ala Val Asn Lys Ile Asp Leu Val Asp Trp Ser Glu 145 150 155 160 Ala Val Phe Arg Asp Ile Glu Ala Glu Phe Thr Ser Leu Ala Thr Asp 165 170 175 Val Gly Val Asp Asp Ala His Val Ile Pro Val Ser Ala Leu Leu Gly 180 185 190 Asp Asn Val Val Glu Pro Ser Ala Asn Leu Pro Trp Tyr Glu Gly Pro 195 200 205 Thr Val Leu Glu Leu Leu Glu Ser Val Glu Val His Arg Gly Arg Ala 210 215 220 His Ala Leu Gly Phe Arg Leu Pro Ile Gln Tyr Val Ile Arg Glu His 225 230 235 240 Ser Ser Asp Tyr Arg Gly Tyr Ala Gly Arg Val Asp Ala Gly Val Leu 245 250 255 Thr Val Gly Asp Thr Val His Leu Pro Glu Gly Arg Thr Ser Thr Val 260 265 270 Thr His Ile Asp Thr Ala Asp Gly Glu Val Arg Ser Ala His Ala Gly 275 280 285 Asp Ser Val Val Leu Arg Leu Ala Asp Asp Ile Asp Leu Ile Arg Gly 290 295 300 Asp Leu Ile Ala Gly Glu Asp Arg Pro Glu Pro Thr Arg Ser Phe Ala 305 310 315 320 Ala Thr Leu Val Gly Leu Gly Asp Arg Thr Leu Arg Pro Gly Ser Pro 325 330 335 Val Lys Val Arg Tyr Gly Thr Ser Leu Val Arg Gly Arg Val Ala Ala 340 345 350 Ile Glu Arg Leu Leu Asp Ile Asp Gly Arg Thr Asp His Leu Ser Pro 355 360 365 Asp Asp Leu Gly Leu Asn Asp Ile Ala His Val Arg Ile Asp Val Ala 370 375 380 Thr Glu Leu Pro Val Glu Pro Tyr Ala Ala Arg Gly Ala Val Gly Ser 385 390 395 400 Phe Leu Leu Ile Asp Gln Ala Ser Gly Asp Thr Leu Ala Ala Gly Leu 405 410 415 Val Gly His Arg Leu Arg 420 <210> 83 <211> 1248 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(948) <223> gene cysY <400> 83 ggactcggcg accgcaccct gcgccccggc tcccccgtga aggtccgcta cggcacctca 60 ctcgtgcggg gacgcgtcgc ggcgatcgag cggctgctcg acatcgacgg ccgcaccgac 120 cacctctccc cggacgacct cggcctcaac gacatcgccc acgtgcgtat cgacgtcgcc 180 accgagctcc ccgtcgagcc ctacgccgcc cgcggcgccg tcggctcctt cctgctcatc 240 gaccaggcca gcggcgacac cctcgccgcc gggctcgtcg gccaccggct ccgctgaccc 300 gtg acc gcc ctg ctc acc ctc tcc cac ggg tcc cgc cac ccg cgc gcc 348 Val Thr Ala Leu Leu Thr Leu Ser His Gly Ser Arg His Pro Arg Ala 1 5 10 15 gcc gcc ggc gtc gac cgc ctc acc cgg gcg gcc ggg gag gtg ctg ggc 396 Ala Ala Gly Val Asp Arg Leu Thr Arg Ala Ala Gly Glu Val Leu Gly 20 25 30 gtc acc gcc cgc gcc gcc cac ctc gag ttc gac acc ccc tcc ctc atc 444 Val Thr Ala Arg Ala Ala His Leu Glu Phe Asp Thr Pro Ser Leu Ile 35 40 45 gac gcc gcc cgc gac ctc gtc gcc cgc ggc cac gac cac gcc gtg gtc 492 Asp Ala Ala Arg Asp Leu Val Ala Arg Gly His Asp His Ala Val Val 50 55 60 gtc ccc ctg ctg ttc acc gac gcc ttc cac gcg cgt cac gac gtc ccc 540 Val Pro Leu Leu Phe Thr Asp Ala Phe His Ala Arg His Asp Val Pro 65 70 75 80 gcc gcc ctc gac gcg gcc cgc cgc gcc acc ggc ctg cgg ctc acg ccc 588 Ala Ala Leu Asp Ala Ala Arg Arg Ala Thr Gly Leu Arg Leu Thr Pro 85 90 95 gcc cgg ggc ctc ggc acc ggc ccc gac atc gcc gac gtc ctt gcc cgc 636 Ala Arg Gly Leu Gly Thr Gly Pro Asp Ile Ala Asp Val Leu Ala Arg 100 105 110 cac acc cgc ccc cgc aac ccg gac ctc gtc ctc ggg cac gtc gga tcc 684 His Thr Arg Pro Arg Asn Pro Asp Leu Val Leu Gly His Val Gly Ser 115 120 125 tcc tcc gtc gca gcc gag gcc gcc gtc cgg tcg ctg gcc gac ctc ctc 732 Ser Ser Val Ala Ala Glu Ala Ala Val Arg Ser Leu Ala Asp Leu Leu 130 135 140 gcc gcc cgc ctc ggg cgg gac gtc gcg gtg gtg cgg gcg acg ggg gag 780 Ala Ala Arg Leu Gly Arg Asp Val Ala Val Val Arg Ala Thr Gly Glu 145 150 155 160 aag gcg gcg tcg ata agc agc ggt gcc cac ctc gtc ccc ctc ttc gtc 828 Lys Ala Ala Ser Ile Ser Ser Gly Ala His Leu Val Pro Leu Phe Val 165 170 175 acc gac gcc ctg ctg ctc gac cgc ctg cgc gcc gcc caa ccc cac gcg 876 Thr Asp Ala Leu Leu Leu Asp Arg Leu Arg Ala Ala Gln Pro His Ala 180 185 190 acc tcc gac cgc ccg ctc acc gac gcc ctc cgc gac gtc gtg gcg gcg 924 Thr Ser Asp Arg Pro Leu Thr Asp Ala Leu Arg Asp Val Val Ala Ala 195 200 205 cgg tac cgg gcc gcc ctc acc tag gaacccacca tgaacacact cctgctcatc 978 Arg Tyr Arg Ala Ala Leu Thr 210 215 gccctcgcgg ggctcgccgc ccagctcgtc gacggcggcc tcggcatggg cttcggcgcc 1038 acctccacca cgatcctcat cgtgctcgcc ggactcggcc ccgccacagc cagcgccgtc 1098 gtccacaccg cggaactggg caccaccctc gtctccggcg tctcgcactg gcgtttcgga 1158 aacgtcgact ggcaggtcgt ccgccgcctc gccatccccg gtgccgtggg cgccttcgcc 1218 ggggcaaccg tgctctccca cttatcgacg 1248 <210> 84 <211> 215 <212> PRT <213> Corynebacterium humireducens <400> 84 Val Thr Ala Leu Leu Thr Leu Ser His Gly Ser Arg His Pro Arg Ala 1 5 10 15 Ala Ala Gly Val Asp Arg Leu Thr Arg Ala Ala Gly Glu Val Leu Gly 20 25 30 Val Thr Ala Arg Ala Ala His Leu Glu Phe Asp Thr Pro Ser Leu Ile 35 40 45 Asp Ala Ala Arg Asp Leu Val Ala Arg Gly His Asp His Ala Val Val 50 55 60 Val Pro Leu Leu Phe Thr Asp Ala Phe His Ala Arg His Asp Val Pro 65 70 75 80 Ala Ala Leu Asp Ala Ala Arg Arg Ala Thr Gly Leu Arg Leu Thr Pro 85 90 95 Ala Arg Gly Leu Gly Thr Gly Pro Asp Ile Ala Asp Val Leu Ala Arg 100 105 110 His Thr Arg Pro Arg Asn Pro Asp Leu Val Leu Gly His Val Gly Ser 115 120 125 Ser Ser Val Ala Ala Glu Ala Ala Val Arg Ser Leu Ala Asp Leu Leu 130 135 140 Ala Ala Arg Leu Gly Arg Asp Val Ala Val Val Arg Ala Thr Gly Glu 145 150 155 160 Lys Ala Ala Ser Ile Ser Ser Gly Ala His Leu Val Pro Leu Phe Val 165 170 175 Thr Asp Ala Leu Leu Leu Asp Arg Leu Arg Ala Ala Gln Pro His Ala 180 185 190 Thr Ser Asp Arg Pro Leu Thr Asp Ala Leu Arg Asp Val Val Ala Ala 195 200 205 Arg Tyr Arg Ala Ala Leu Thr 210 215 <210> 85 <211> 1515 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1215) <223> gene cysZ <400> 85 gacctcgtcc tcgggcacgt cggatcctcc tccgtcgcag ccgaggccgc cgtccggtcg 60 ctggccgacc tcctcgccgc ccgcctcggg cgggacgtcg cggtggtgcg ggcgacgggg 120 gagaaggcgg cgtcgataag cagcggtgcc cacctcgtcc ccctcttcgt caccgacgcc 180 ctgctgctcg accgcctgcg cgccgcccaa ccccacgcga cctccgaccg cccgctcacc 240 gacgccctcc gcgacgtcgt ggcggcgcgg taccgggccg ccctcaccta ggaacccacc 300 atg aac aca ctc ctg ctc atc gcc ctc gcg ggg ctc gcc gcc cag ctc 348 Met Asn Thr Leu Leu Leu Ile Ala Leu Ala Gly Leu Ala Ala Gln Leu 1 5 10 15 gtc gac ggc ggc ctc ggc atg ggc ttc ggc gcc acc tcc acc acg atc 396 Val Asp Gly Gly Leu Gly Met Gly Phe Gly Ala Thr Ser Thr Thr Ile 20 25 30 ctc atc gtg ctc gcc gga ctc ggc ccc gcc aca gcc agc gcc gtc gtc 444 Leu Ile Val Leu Ala Gly Leu Gly Pro Ala Thr Ala Ser Ala Val Val 35 40 45 cac acc gcg gaa ctg ggc acc acc ctc gtc tcc ggc gtc tcg cac tgg 492 His Thr Ala Glu Leu Gly Thr Thr Leu Val Ser Gly Val Ser His Trp 50 55 60 cgt ttc gga aac gtc gac tgg cag gtc gtc cgc cgc ctc gcc atc ccc 540 Arg Phe Gly Asn Val Asp Trp Gln Val Val Arg Arg Leu Ala Ile Pro 65 70 75 80 ggt gcc gtg ggc gcc ttc gcc ggg gca acc gtg ctc tcc cac tta tcg 588 Gly Ala Val Gly Ala Phe Ala Gly Ala Thr Val Leu Ser His Leu Ser 85 90 95 acg cag gcc gcc gcc ccc gtc acc gcc acg atc ctc acc ctc atc ggc 636 Thr Gln Ala Ala Ala Pro Val Thr Ala Thr Ile Leu Thr Leu Ile Gly 100 105 110 gcc aac ctc gtg tgg cgc ttc tcc cgc gga cgc atc cac ccg cgc cgc 684 Ala Asn Leu Val Trp Arg Phe Ser Arg Gly Arg Ile His Pro Arg Arg 115 120 125 gcc ggg aag ggg cac tcg cgc ggc ttc ctc ggc ggg ctc ggc ctg ttc 732 Ala Gly Lys Gly His Ser Arg Gly Phe Leu Gly Gly Leu Gly Leu Phe 130 135 140 ggc ggc ttc atc gac gcc tcc gga ggc ggc ggc tgg ggc ccg gtg acc 780 Gly Gly Phe Ile Asp Ala Ser Gly Gly Gly Gly Trp Gly Pro Val Thr 145 150 155 160 acc tcg acc ctg atg acg ctg ggg cgg gcg gaa ccg cgc cgc atc gtc 828 Thr Ser Thr Leu Met Thr Leu Gly Arg Ala Glu Pro Arg Arg Ile Val 165 170 175 ggc acg gtc aac acc gcc gag ttc ctg gtc acc gcc gcg gcg acc gcc 876 Gly Thr Val Asn Thr Ala Glu Phe Leu Val Thr Ala Ala Ala Thr Ala 180 185 190 ggc ttc gtc atc ggc ctg tgg gac gac ctg gtg gcc aac ctc gcg gcc 924 Gly Phe Val Ile Gly Leu Trp Asp Asp Leu Val Ala Asn Leu Ala Ala 195 200 205 gtg ctc gcg ctg ctc atc ggc ggt gtc atc gcc gcg ccg gtc gcc gcg 972 Val Leu Ala Leu Leu Ile Gly Gly Val Ile Ala Ala Pro Val Ala Ala 210 215 220 tgg ctg gtc acc cgc ctc aac ccg gcc gcc ctc ggc ggc gtc gtg ggc 1020 Trp Leu Val Thr Arg Leu Asn Pro Ala Ala Leu Gly Gly Val Val Gly 225 230 235 240 acc ctg ctg gtc gcg ctg aac ctc ccc aag gtg ctg ccc ttc gcc gga 1068 Thr Leu Leu Val Ala Leu Asn Leu Pro Lys Val Leu Pro Phe Ala Gly 245 250 255 gga ccg gtg ctg ctg gcc gtc atc gtc gtc ggc ctg ggc ttc tcg tgg 1116 Gly Pro Val Leu Leu Ala Val Ile Val Val Gly Leu Gly Phe Ser Trp 260 265 270 gtc ggc tgg cga cgc tcc ctc cgt gcc cgc gcc gcc gcc ggg cag ccc 1164 Val Gly Trp Arg Arg Ser Leu Arg Ala Arg Ala Ala Ala Gly Gln Pro 275 280 285 gtc acc gga acg acg agc acc ccc acc gga cag tcg gtg ggg gtg cgg 1212 Val Thr Gly Thr Thr Ser Thr Pro Thr Gly Gln Ser Val Gly Val Arg 290 295 300 tag ggcgggggct acagggcggc gacgtcgccg atgacgtaca cggccgggga 1265 cccgatgcgg ttcccggcca tcgtcgtggc cagggttccc aacgtgcacc ggaaggaacg 1325 ctgcccagtg gtggtgccct cctggatgac ggcggccggg gtgtgggggt cgaggccggc 1385 gtcgacaagc gtggcggtga tggcggcgac gttgcgcaca cccatgatca ccgagatcgt 1445 gccgcccgcc cgcgccagcg cggcccagtc gacgagcgag tccgggtgcc ccggcggcag 1505 atgcccggag 1515 <210> 86 <211> 304 <212> PRT <213> Corynebacterium humireducens <400> 86 Met Asn Thr Leu Leu Leu Ile Ala Leu Ala Gly Leu Ala Ala Gln Leu 1 5 10 15 Val Asp Gly Gly Leu Gly Met Gly Phe Gly Ala Thr Ser Thr Thr Ile 20 25 30 Leu Ile Val Leu Ala Gly Leu Gly Pro Ala Thr Ala Ser Ala Val Val 35 40 45 His Thr Ala Glu Leu Gly Thr Thr Leu Val Ser Gly Val Ser His Trp 50 55 60 Arg Phe Gly Asn Val Asp Trp Gln Val Val Arg Arg Leu Ala Ile Pro 65 70 75 80 Gly Ala Val Gly Ala Phe Ala Gly Ala Thr Val Leu Ser His Leu Ser 85 90 95 Thr Gln Ala Ala Ala Pro Val Thr Ala Thr Ile Leu Thr Leu Ile Gly 100 105 110 Ala Asn Leu Val Trp Arg Phe Ser Arg Gly Arg Ile His Pro Arg Arg 115 120 125 Ala Gly Lys Gly His Ser Arg Gly Phe Leu Gly Gly Leu Gly Leu Phe 130 135 140 Gly Gly Phe Ile Asp Ala Ser Gly Gly Gly Gly Trp Gly Pro Val Thr 145 150 155 160 Thr Ser Thr Leu Met Thr Leu Gly Arg Ala Glu Pro Arg Arg Ile Val 165 170 175 Gly Thr Val Asn Thr Ala Glu Phe Leu Val Thr Ala Ala Ala Thr Ala 180 185 190 Gly Phe Val Ile Gly Leu Trp Asp Asp Leu Val Ala Asn Leu Ala Ala 195 200 205 Val Leu Ala Leu Leu Ile Gly Gly Val Ile Ala Ala Pro Val Ala Ala 210 215 220 Trp Leu Val Thr Arg Leu Asn Pro Ala Ala Leu Gly Gly Val Val Gly 225 230 235 240 Thr Leu Leu Val Ala Leu Asn Leu Pro Lys Val Leu Pro Phe Ala Gly 245 250 255 Gly Pro Val Leu Leu Ala Val Ile Val Val Gly Leu Gly Phe Ser Trp 260 265 270 Val Gly Trp Arg Arg Ser Leu Arg Ala Arg Ala Ala Ala Gly Gln Pro 275 280 285 Val Thr Gly Thr Thr Ser Thr Pro Thr Gly Gln Ser Val Gly Val Arg 290 295 300 <210> 87 <211> 2916 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2616) <223> gene metE <400> 87 gactggaggc ggcaggcccg gagagcgagt accagacggg cctcgacctc acggccgagc 60 tcgcgcagac catcctggac gccggtgccg gggggatgca cgtgtacacg ttcaaccggc 120 cggacaccac caccgaactg ctggaccgca tcggcatcac ccccgaccct cgggttaggg 180 agtgaggcgg gagaccgccg ccgctgcatc tcatgttgat agcgacctgc tgcacgcacc 240 gggagccccc gaagcagacc aggttgtaca cccaagattt tccgaaaggc ctcccttttc 300 atg tct tcc acc gct ttc ccc acc ttc acc atc gag ggc tac ccg cgc 348 Met Ser Ser Thr Ala Phe Pro Thr Phe Thr Ile Glu Gly Tyr Pro Arg 1 5 10 15 gtc ggc gcc aac cgc gag ctc aag aag gcc ctc gag tcc ttc tgg gcc 396 Val Gly Ala Asn Arg Glu Leu Lys Lys Ala Leu Glu Ser Phe Trp Ala 20 25 30 ggc cgc atc gac gag gag acc ttc atc tcc tcc gcg cac tcc atc cgc 444 Gly Arg Ile Asp Glu Glu Thr Phe Ile Ser Ser Ala His Ser Ile Arg 35 40 45 ctg gag aac tac gcc cgt ctg cgt gac ctc ggc ctg gac cag gac tac 492 Leu Glu Asn Tyr Ala Arg Leu Arg Asp Leu Gly Leu Asp Gln Asp Tyr 50 55 60 gcc atc ccg gcc gac gtc gcc ctc tac gac cag gtg ctc gag acc ggc 540 Ala Ile Pro Ala Asp Val Ala Leu Tyr Asp Gln Val Leu Glu Thr Gly 65 70 75 80 gtc acc gtc ggc ctg atc ggt ggc gac gcc gcc gag gtc gac ctc acc 588 Val Thr Val Gly Leu Ile Gly Gly Asp Ala Ala Glu Val Asp Leu Thr 85 90 95 gag tac ttc gcc ctg gcc cgc ggc acc gcc gag cgt tcc ccg ctc gag 636 Glu Tyr Phe Ala Leu Ala Arg Gly Thr Ala Glu Arg Ser Pro Leu Glu 100 105 110 atg acc aag tgg ttc gac acc aac tac cac tac ctc gtc ccg gag gtc 684 Met Thr Lys Trp Phe Asp Thr Asn Tyr His Tyr Leu Val Pro Glu Val 115 120 125 gcc gag gac acc gtc atc acc ccg cgc ccg cag cgc atc ctc gac atc 732 Ala Glu Asp Thr Val Ile Thr Pro Arg Pro Gln Arg Ile Leu Asp Ile 130 135 140 gtc gcc gag gcc aag gag gcc ggc cac acc gtc cgt ccg ttc ctg gtc 780 Val Ala Glu Ala Lys Glu Ala Gly His Thr Val Arg Pro Phe Leu Val 145 150 155 160 ggc ccg gtc acc ctg ctg gcc aag tcc aag gcc gcc gag aac gcc ggc 828 Gly Pro Val Thr Leu Leu Ala Lys Ser Lys Ala Ala Glu Asn Ala Gly 165 170 175 gag ggc ttc aac ccg ctg tcc cgc ctc gag gac ctc acc gcc gcc tac 876 Glu Gly Phe Asn Pro Leu Ser Arg Leu Glu Asp Leu Thr Ala Ala Tyr 180 185 190 gcc cag gtc ctc gcc gcc ctc aag gag gcc ggc gtg gag tgg gtc cag 924 Ala Gln Val Leu Ala Ala Leu Lys Glu Ala Gly Val Glu Trp Val Gln 195 200 205 ctc gcc gag ccg gcc ctg gtc gcc gac ctg acc atc cgt tcc gac gcc 972 Leu Ala Glu Pro Ala Leu Val Ala Asp Leu Thr Ile Arg Ser Asp Ala 210 215 220 gac ctg gcc gcc gac gcg aag gcc acc tac gag gcg ctc ctc ggc tcc 1020 Asp Leu Ala Ala Asp Ala Lys Ala Thr Tyr Glu Ala Leu Leu Gly Ser 225 230 235 240 gag gac cgc ccg cag gtc ctc atc acc acc ccc tac ggt gcc ctg cgc 1068 Glu Asp Arg Pro Gln Val Leu Ile Thr Thr Pro Tyr Gly Ala Leu Arg 245 250 255 aac ggc ctc gac gcc ctc gtc gcc gcc aag ccg gag gcc ctg cag gtc 1116 Asn Gly Leu Asp Ala Leu Val Ala Ala Lys Pro Glu Ala Leu Gln Val 260 265 270 gac ctc gca ccg gtc acc ctc gcc aac gag gag ggc tac gtc gag cgc 1164 Asp Leu Ala Pro Val Thr Leu Ala Asn Glu Glu Gly Tyr Val Glu Arg 275 280 285 gtc gcc aag gca gtc gcc ggc acc ggc atc acc ctg gcc gcc ggc gtc 1212 Val Ala Lys Ala Val Ala Gly Thr Gly Ile Thr Leu Ala Ala Gly Val 290 295 300 atc gac ggc cgc aac atc tgg gcc gcc gac ctg cgc gag cgc ctc tcc 1260 Ile Asp Gly Arg Asn Ile Trp Ala Ala Asp Leu Arg Glu Arg Leu Ser 305 310 315 320 gtc ctg gag gag ctg aag aag ggc ggc gtc gag aag atc gcc gtc acc 1308 Val Leu Glu Glu Leu Lys Lys Gly Gly Val Glu Lys Ile Ala Val Thr 325 330 335 tcc tcc gtc tcc ctg cag cac gtc ccg cac acc gtc tcc gtg gag aag 1356 Ser Ser Val Ser Leu Gln His Val Pro His Thr Val Ser Val Glu Lys 340 345 350 aac ctc ccg gtc gac gtc gcc ggc tgg ctc tcc ttc gcc gac gag aag 1404 Asn Leu Pro Val Asp Val Ala Gly Trp Leu Ser Phe Ala Asp Glu Lys 355 360 365 atc ggc gag gca cag gcc ctg gcc acc gca ctc gag ggc ggc tcc gtc 1452 Ile Gly Glu Ala Gln Ala Leu Ala Thr Ala Leu Glu Gly Gly Ser Val 370 375 380 gcc gcc gca gac gcc ttc gcc cgc tcc gac cgc gcc gtg cgc acc cgt 1500 Ala Ala Ala Asp Ala Phe Ala Arg Ser Asp Arg Ala Val Arg Thr Arg 385 390 395 400 cgt gag tcc gcc cgc acc cac aac cag gcc gtc cag gac cgc gtc gcc 1548 Arg Glu Ser Ala Arg Thr His Asn Gln Ala Val Gln Asp Arg Val Ala 405 410 415 gct ctg ccg gac ggc cag gtc gca cgc gag ccg gag ttc gag ggc cgc 1596 Ala Leu Pro Asp Gly Gln Val Ala Arg Glu Pro Glu Phe Glu Gly Arg 420 425 430 gtc gag gca cag aag gtg ctc aac ctg ccg aag ctg ccg acc acc acg 1644 Val Glu Ala Gln Lys Val Leu Asn Leu Pro Lys Leu Pro Thr Thr Thr 435 440 445 atc ggc tcc ttc ccg cag acc acc gag atc cgc aag gca cgc gcc gac 1692 Ile Gly Ser Phe Pro Gln Thr Thr Glu Ile Arg Lys Ala Arg Ala Asp 450 455 460 cac cgc tcc ggc gcc ctc acc gac gag cag tac acc gac gcc ctc aag 1740 His Arg Ser Gly Ala Leu Thr Asp Glu Gln Tyr Thr Asp Ala Leu Lys 465 470 475 480 gac gag gtc aag tcc gtc atc gag ctg cag gag cgc ctc ggc atc gac 1788 Asp Glu Val Lys Ser Val Ile Glu Leu Gln Glu Arg Leu Gly Ile Asp 485 490 495 gtc ctc gtc cac ggc gag ccc gag cgc aac gac atg gtc cag tac ttc 1836 Val Leu Val His Gly Glu Pro Glu Arg Asn Asp Met Val Gln Tyr Phe 500 505 510 gcc gag ctt ctc gac ggc ttc gtg acc acc gag cac ggc tgg gtc cag 1884 Ala Glu Leu Leu Asp Gly Phe Val Thr Thr Glu His Gly Trp Val Gln 515 520 525 tcc tac ggc tcc cgc tgc acc cgc ccg tcc atc gtc gtc ggc gac gtc 1932 Ser Tyr Gly Ser Arg Cys Thr Arg Pro Ser Ile Val Val Gly Asp Val 530 535 540 tcc cgc ccg aag gcc atg acc gtc gag tgg gcc aag tac gcc cag tcc 1980 Ser Arg Pro Lys Ala Met Thr Val Glu Trp Ala Lys Tyr Ala Gln Ser 545 550 555 560 ctc tcc gac aag cac gtc aag ggc atg ctg acc ggc ccg gtc acc atc 2028 Leu Ser Asp Lys His Val Lys Gly Met Leu Thr Gly Pro Val Thr Ile 565 570 575 ctc gcg tgg tcc ttc acc cgt gac gac gtt ccg aag tcc gtc tcc gcc 2076 Leu Ala Trp Ser Phe Thr Arg Asp Asp Val Pro Lys Ser Val Ser Ala 580 585 590 gac cag atc ggc gtc gcc ctc gcc gac gag gtc gcc gac ctc gag gcc 2124 Asp Gln Ile Gly Val Ala Leu Ala Asp Glu Val Ala Asp Leu Glu Ala 595 600 605 gcc ggc atc aac gtc atc cag atc gac gag ccg gcc ctg cgc gag ctg 2172 Ala Gly Ile Asn Val Ile Gln Ile Asp Glu Pro Ala Leu Arg Glu Leu 610 615 620 ctg ccg ctg cgt gag gag gac cgc ccg gca tac ctg gac tgg gcc gtc 2220 Leu Pro Leu Arg Glu Glu Asp Arg Pro Ala Tyr Leu Asp Trp Ala Val 625 630 635 640 cgc tcc ttc cgc ctc gtc tcc ctg gac gcg aag ccg acc acc cag atc 2268 Arg Ser Phe Arg Leu Val Ser Leu Asp Ala Lys Pro Thr Thr Gln Ile 645 650 655 cac acc cac ctc tgc tac tcg gag ttc ggg cag atc atc gac gcc gtc 2316 His Thr His Leu Cys Tyr Ser Glu Phe Gly Gln Ile Ile Asp Ala Val 660 665 670 gcc gcc ctc gac gcc gac gtc acc tcc atc gag gcc gcc cgc tcc cgc 2364 Ala Ala Leu Asp Ala Asp Val Thr Ser Ile Glu Ala Ala Arg Ser Arg 675 680 685 atg gag ctg ctg ggt gac ctg gac gag tcc ttc cac tcc gag atc ggc 2412 Met Glu Leu Leu Gly Asp Leu Asp Glu Ser Phe His Ser Glu Ile Gly 690 695 700 ccg ggc atc tgg gac atc cac tcc ccg cgc gtc ccg gac gtc gcc gag 2460 Pro Gly Ile Trp Asp Ile His Ser Pro Arg Val Pro Asp Val Ala Glu 705 710 715 720 ctc acc gag ctg atc aag gcc gcc ctg gtc aac gtc ccg acc gag cgc 2508 Leu Thr Glu Leu Ile Lys Ala Ala Leu Val Asn Val Pro Thr Glu Arg 725 730 735 ctc tgg gtc aac ccg gac tgc ggt ctg aag acc cgc ggc tac gcc gag 2556 Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Tyr Ala Glu 740 745 750 acc gag gcg tcc ctg cgc aac atg gtc ctc gcc cgc gac ctc gtg gtc 2604 Thr Glu Ala Ser Leu Arg Asn Met Val Leu Ala Arg Asp Leu Val Val 755 760 765 gag tcc ctg taa cacctgcgta gcaactacgt tgcgggcccg gttccacttc 2656 Glu Ser Leu 770 ggtggggccg ggcccctcgt gattccgggg gcggctagcg ctcgtcgatg acctccgcga 2716 aggcggccac cttccggcgg agatcctcga tgagggcgtc cccgtccctc cgggacttct 2776 cctccgacgc cgggtcatcc cagcctgtct cccagtggtg gtgggaccgc cagtggtcgc 2836 accatgcgta gagccgctgc gtcagcgact cggacaggcc gtagtcccgt ggcgccatca 2896 gataggtgtc cgtgccgttc 2916 <210> 88 <211> 771 <212> PRT <213> Corynebacterium humireducens <400> 88 Met Ser Ser Thr Ala Phe Pro Thr Phe Thr Ile Glu Gly Tyr Pro Arg 1 5 10 15 Val Gly Ala Asn Arg Glu Leu Lys Lys Ala Leu Glu Ser Phe Trp Ala 20 25 30 Gly Arg Ile Asp Glu Glu Thr Phe Ile Ser Ser Ala His Ser Ile Arg 35 40 45 Leu Glu Asn Tyr Ala Arg Leu Arg Asp Leu Gly Leu Asp Gln Asp Tyr 50 55 60 Ala Ile Pro Ala Asp Val Ala Leu Tyr Asp Gln Val Leu Glu Thr Gly 65 70 75 80 Val Thr Val Gly Leu Ile Gly Gly Asp Ala Ala Glu Val Asp Leu Thr 85 90 95 Glu Tyr Phe Ala Leu Ala Arg Gly Thr Ala Glu Arg Ser Pro Leu Glu 100 105 110 Met Thr Lys Trp Phe Asp Thr Asn Tyr His Tyr Leu Val Pro Glu Val 115 120 125 Ala Glu Asp Thr Val Ile Thr Pro Arg Pro Gln Arg Ile Leu Asp Ile 130 135 140 Val Ala Glu Ala Lys Glu Ala Gly His Thr Val Arg Pro Phe Leu Val 145 150 155 160 Gly Pro Val Thr Leu Leu Ala Lys Ser Lys Ala Ala Glu Asn Ala Gly 165 170 175 Glu Gly Phe Asn Pro Leu Ser Arg Leu Glu Asp Leu Thr Ala Ala Tyr 180 185 190 Ala Gln Val Leu Ala Ala Leu Lys Glu Ala Gly Val Glu Trp Val Gln 195 200 205 Leu Ala Glu Pro Ala Leu Val Ala Asp Leu Thr Ile Arg Ser Asp Ala 210 215 220 Asp Leu Ala Ala Asp Ala Lys Ala Thr Tyr Glu Ala Leu Leu Gly Ser 225 230 235 240 Glu Asp Arg Pro Gln Val Leu Ile Thr Thr Pro Tyr Gly Ala Leu Arg 245 250 255 Asn Gly Leu Asp Ala Leu Val Ala Ala Lys Pro Glu Ala Leu Gln Val 260 265 270 Asp Leu Ala Pro Val Thr Leu Ala Asn Glu Glu Gly Tyr Val Glu Arg 275 280 285 Val Ala Lys Ala Val Ala Gly Thr Gly Ile Thr Leu Ala Ala Gly Val 290 295 300 Ile Asp Gly Arg Asn Ile Trp Ala Ala Asp Leu Arg Glu Arg Leu Ser 305 310 315 320 Val Leu Glu Glu Leu Lys Lys Gly Gly Val Glu Lys Ile Ala Val Thr 325 330 335 Ser Ser Val Ser Leu Gln His Val Pro His Thr Val Ser Val Glu Lys 340 345 350 Asn Leu Pro Val Asp Val Ala Gly Trp Leu Ser Phe Ala Asp Glu Lys 355 360 365 Ile Gly Glu Ala Gln Ala Leu Ala Thr Ala Leu Glu Gly Gly Ser Val 370 375 380 Ala Ala Ala Asp Ala Phe Ala Arg Ser Asp Arg Ala Val Arg Thr Arg 385 390 395 400 Arg Glu Ser Ala Arg Thr His Asn Gln Ala Val Gln Asp Arg Val Ala 405 410 415 Ala Leu Pro Asp Gly Gln Val Ala Arg Glu Pro Glu Phe Glu Gly Arg 420 425 430 Val Glu Ala Gln Lys Val Leu Asn Leu Pro Lys Leu Pro Thr Thr Thr 435 440 445 Ile Gly Ser Phe Pro Gln Thr Thr Glu Ile Arg Lys Ala Arg Ala Asp 450 455 460 His Arg Ser Gly Ala Leu Thr Asp Glu Gln Tyr Thr Asp Ala Leu Lys 465 470 475 480 Asp Glu Val Lys Ser Val Ile Glu Leu Gln Glu Arg Leu Gly Ile Asp 485 490 495 Val Leu Val His Gly Glu Pro Glu Arg Asn Asp Met Val Gln Tyr Phe 500 505 510 Ala Glu Leu Leu Asp Gly Phe Val Thr Thr Glu His Gly Trp Val Gln 515 520 525 Ser Tyr Gly Ser Arg Cys Thr Arg Pro Ser Ile Val Val Gly Asp Val 530 535 540 Ser Arg Pro Lys Ala Met Thr Val Glu Trp Ala Lys Tyr Ala Gln Ser 545 550 555 560 Leu Ser Asp Lys His Val Lys Gly Met Leu Thr Gly Pro Val Thr Ile 565 570 575 Leu Ala Trp Ser Phe Thr Arg Asp Asp Val Pro Lys Ser Val Ser Ala 580 585 590 Asp Gln Ile Gly Val Ala Leu Ala Asp Glu Val Ala Asp Leu Glu Ala 595 600 605 Ala Gly Ile Asn Val Ile Gln Ile Asp Glu Pro Ala Leu Arg Glu Leu 610 615 620 Leu Pro Leu Arg Glu Glu Asp Arg Pro Ala Tyr Leu Asp Trp Ala Val 625 630 635 640 Arg Ser Phe Arg Leu Val Ser Leu Asp Ala Lys Pro Thr Thr Gln Ile 645 650 655 His Thr His Leu Cys Tyr Ser Glu Phe Gly Gln Ile Ile Asp Ala Val 660 665 670 Ala Ala Leu Asp Ala Asp Val Thr Ser Ile Glu Ala Ala Arg Ser Arg 675 680 685 Met Glu Leu Leu Gly Asp Leu Asp Glu Ser Phe His Ser Glu Ile Gly 690 695 700 Pro Gly Ile Trp Asp Ile His Ser Pro Arg Val Pro Asp Val Ala Glu 705 710 715 720 Leu Thr Glu Leu Ile Lys Ala Ala Leu Val Asn Val Pro Thr Glu Arg 725 730 735 Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Tyr Ala Glu 740 745 750 Thr Glu Ala Ser Leu Arg Asn Met Val Leu Ala Arg Asp Leu Val Val 755 760 765 Glu Ser Leu 770 <210> 89 <211> 1200 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(900) <223> gene ptH1 <400> 89 gcgaacttgg cgtcgaagtc gttcgacagg tcgacctccg gcagggggcg gtcgccggcg 60 acacggcgga cgtcggcaag cgactcgaag ggcgcctgcc gcgtgaacag attgacggcg 120 tagggccccg tgacctgcgc cagctgctcc tccaggagcg cggcgggcgc gccgcccggc 180 gcgaggaaac cgagggaacc ggcggctgcg gcggcgtcga caagcgcggg cgacgagggg 240 ccgccggcca tgggcgcgac gatgatctgc gggatgtcca tgtgtgagag actatcgggc 300 gtg ttc aag atc ttc gac ctc ttc cgc cgc aag cag acg acc ccg gcg 348 Val Phe Lys Ile Phe Asp Leu Phe Arg Arg Lys Gln Thr Thr Pro Ala 1 5 10 15 ctc agc gcc gac tgg atc atc ctc ggc ctg ggc aac ccg ggg ccg aaa 396 Leu Ser Ala Asp Trp Ile Ile Leu Gly Leu Gly Asn Pro Gly Pro Lys 20 25 30 tac gcc gcc acg cgc cac aac gtg ggc tac atg gcc gtc gac gag ctt 444 Tyr Ala Ala Thr Arg His Asn Val Gly Tyr Met Ala Val Asp Glu Leu 35 40 45 ctc gac gcc acc ccg ctc cag ccc ctc ccc ggt acg aag gca ctg cac 492 Leu Asp Ala Thr Pro Leu Gln Pro Leu Pro Gly Thr Lys Ala Leu His 50 55 60 gcg aag acg atg atc gcg ggg cag gac gtg ctc gtg ctg cgt tcg acg 540 Ala Lys Thr Met Ile Ala Gly Gln Asp Val Leu Val Leu Arg Ser Thr 65 70 75 80 acc tac atg aac ctc tcc ggc gag gct gtc gcc cca ctc gcg gag cag 588 Thr Tyr Met Asn Leu Ser Gly Glu Ala Val Ala Pro Leu Ala Glu Gln 85 90 95 ctc ggg gtg ccg gcg gag cgg atc atc gcc atc cac gac gag ctc gac 636 Leu Gly Val Pro Ala Glu Arg Ile Ile Ala Ile His Asp Glu Leu Asp 100 105 110 ctg ccg gtg ggc acc gtg cgg gtg aag aag ggc ggc aac gag aac ggc 684 Leu Pro Val Gly Thr Val Arg Val Lys Lys Gly Gly Asn Glu Asn Gly 115 120 125 cac aac ggc ctg aag tcc atg acc gag cac ctg ggc acc cgc gac tac 732 His Asn Gly Leu Lys Ser Met Thr Glu His Leu Gly Thr Arg Asp Tyr 130 135 140 ctg cgg gtg cgc atc ggc atc gcc cgc ccg ccg cag ggc gtg tcg gtg 780 Leu Arg Val Arg Ile Gly Ile Ala Arg Pro Pro Gln Gly Val Ser Val 145 150 155 160 acc gac cat gtc ctc ggc ccc atc gac ggc ggc gag aac atc cgc ctg 828 Thr Asp His Val Leu Gly Pro Ile Asp Gly Gly Glu Asn Ile Arg Leu 165 170 175 gcc gcc gag gcg gcg cgg ctc atc gtc gag cag ggg ctg ggc agg gcg 876 Ala Ala Glu Ala Ala Arg Leu Ile Val Glu Gln Gly Leu Gly Arg Ala 180 185 190 cag aac gtc atc cac gcg cgg tga tcgcccccgg gcgctgctgc accaaagggg 930 Gln Asn Val Ile His Ala Arg 195 gtattcgagt cggggcattg atcgggtgac gcccacgcgc tacccgatca atgcctcagt 990 tcgaataccc cctcggcccc ggcggccccg caggatctac ttcgccatct gcgcgacgag 1050 cttctccagg tcgccgagca tcgtctccag ctcgccgagg cgacgctcct gcggggagaa 1110 cacgccctcc tccgcgtcgt tgaaggtcga caggtacacc tggttgcgca cgtcgtactg 1170 ctggaagttg ccgacgatct gccgccagct 1200 <210> 90 <211> 199 <212> PRT <213> Corynebacterium humireducens <400> 90 Val Phe Lys Ile Phe Asp Leu Phe Arg Arg Lys Gln Thr Thr Pro Ala 1 5 10 15 Leu Ser Ala Asp Trp Ile Ile Leu Gly Leu Gly Asn Pro Gly Pro Lys 20 25 30 Tyr Ala Ala Thr Arg His Asn Val Gly Tyr Met Ala Val Asp Glu Leu 35 40 45 Leu Asp Ala Thr Pro Leu Gln Pro Leu Pro Gly Thr Lys Ala Leu His 50 55 60 Ala Lys Thr Met Ile Ala Gly Gln Asp Val Leu Val Leu Arg Ser Thr 65 70 75 80 Thr Tyr Met Asn Leu Ser Gly Glu Ala Val Ala Pro Leu Ala Glu Gln 85 90 95 Leu Gly Val Pro Ala Glu Arg Ile Ile Ala Ile His Asp Glu Leu Asp 100 105 110 Leu Pro Val Gly Thr Val Arg Val Lys Lys Gly Gly Asn Glu Asn Gly 115 120 125 His Asn Gly Leu Lys Ser Met Thr Glu His Leu Gly Thr Arg Asp Tyr 130 135 140 Leu Arg Val Arg Ile Gly Ile Ala Arg Pro Pro Gln Gly Val Ser Val 145 150 155 160 Thr Asp His Val Leu Gly Pro Ile Asp Gly Gly Glu Asn Ile Arg Leu 165 170 175 Ala Ala Glu Ala Ala Arg Leu Ile Val Glu Gln Gly Leu Gly Arg Ala 180 185 190 Gln Asn Val Ile His Ala Arg 195 <210> 91 <211> 1146 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(846) <223> gene ptH2 <400> 91 aggagatcgt cgtgtccgtc gagggcatcg aggccggcaa cgtcatcaac gccggtgaca 60 tcaccctgcc ggaggacgtc accctggtcg acgacgccga gctgctcgtc ttcaacatca 120 ccttcgagga ggtcgccgac gttccggagg agggcgagga ggccaccgaa gacgctgtcg 180 gcgaggccgg caaggacgag gctgccgagg gcgaggagta gtcactcccc ttccgccacg 240 tgtgacgccc ctgaccggcc ggtcaggggc gtctcccctt ttctgtgcca gactgatcgg 300 gtg act gat tct cca ctt ctc atc gtc ggc ctg ggt aac ccg ggc gcg 348 Val Thr Asp Ser Pro Leu Leu Ile Val Gly Leu Gly Asn Pro Gly Ala 1 5 10 15 aag tac gcc gac acc cgc cac aac atc ggc gtc atg ctt ctc gac gag 396 Lys Tyr Ala Asp Thr Arg His Asn Ile Gly Val Met Leu Leu Asp Glu 20 25 30 ctc gcc tcc cgc atc acc ccc gtc ccc gcc agc ttc gcc gtg cac aag 444 Leu Ala Ser Arg Ile Thr Pro Val Pro Ala Ser Phe Ala Val His Lys 35 40 45 cgc acg aac acc gag gtc gcc gag gtc cgt cat cgc ggc cgt cgc ctc 492 Arg Thr Asn Thr Glu Val Ala Glu Val Arg His Arg Gly Arg Arg Leu 50 55 60 gtg ctg gcc cgg acc cgc ggc tac atg aac gtc tcc ggt gga ccg gtg 540 Val Leu Ala Arg Thr Arg Gly Tyr Met Asn Val Ser Gly Gly Pro Val 65 70 75 80 aag gca ctg gcg acc ttc ttc agg atc gcc ccc ggc gac atc atc gtc 588 Lys Ala Leu Ala Thr Phe Phe Arg Ile Ala Pro Gly Asp Ile Ile Val 85 90 95 gcc cac gac gaa ctc gag ctg gac ttc ggc gag cac cgc ctc cgc acc 636 Ala His Asp Glu Leu Glu Leu Asp Phe Gly Glu His Arg Leu Arg Thr 100 105 110 ggc ggc ggc gac cac ggc cac aac ggc ctg aag tcc gtg acc aag tcg 684 Gly Gly Gly Asp His Gly His Asn Gly Leu Lys Ser Val Thr Lys Ser 115 120 125 ctg gga acg aag gag tac cag cgc ctg gcg ctc ggc atc ggc cgt ccg 732 Leu Gly Thr Lys Glu Tyr Gln Arg Leu Ala Leu Gly Ile Gly Arg Pro 130 135 140 ccc ggg cgt atg gac ccc gcc gcc ttc gtg ctc aag ccc ttc acc cgc 780 Pro Gly Arg Met Asp Pro Ala Ala Phe Val Leu Lys Pro Phe Thr Arg 145 150 155 160 cag gag gcc ggg gag ctg ccc ttc ctg ctc tcc gac gcc gcc gac gaa 828 Gln Glu Ala Gly Glu Leu Pro Phe Leu Leu Ser Asp Ala Ala Asp Glu 165 170 175 ctc gaa cgc gcc ctc tga cctcccaggg tggaacctgg tcccggaagg 876 Leu Glu Arg Ala Leu 180 ttccggacgc tgcccgggtg tctagatggt gagctcctcc ggggccgcct ccccctcgcg 936 ccgggcgatg atcttgtgga tctggtactc ccactcctgc agctcctccg gcagctcccc 996 cttcgagggg tcacggagat actcgcgacg cccggcgtcg tagccccaca gccagtcgac 1056 gtagcggtac caggacgtgc ggccggcctg gcggaaggtc tcgttgcgga tcaggcggga 1116 gacgccgtcg acgtgccaca gctcccccca 1146 <210> 92 <211> 181 <212> PRT <213> Corynebacterium humireducens <400> 92 Val Thr Asp Ser Pro Leu Leu Ile Val Gly Leu Gly Asn Pro Gly Ala 1 5 10 15 Lys Tyr Ala Asp Thr Arg His Asn Ile Gly Val Met Leu Leu Asp Glu 20 25 30 Leu Ala Ser Arg Ile Thr Pro Val Pro Ala Ser Phe Ala Val His Lys 35 40 45 Arg Thr Asn Thr Glu Val Ala Glu Val Arg His Arg Gly Arg Arg Leu 50 55 60 Val Leu Ala Arg Thr Arg Gly Tyr Met Asn Val Ser Gly Gly Pro Val 65 70 75 80 Lys Ala Leu Ala Thr Phe Phe Arg Ile Ala Pro Gly Asp Ile Ile Val 85 90 95 Ala His Asp Glu Leu Glu Leu Asp Phe Gly Glu His Arg Leu Arg Thr 100 105 110 Gly Gly Gly Asp His Gly His Asn Gly Leu Lys Ser Val Thr Lys Ser 115 120 125 Leu Gly Thr Lys Glu Tyr Gln Arg Leu Ala Leu Gly Ile Gly Arg Pro 130 135 140 Pro Gly Arg Met Asp Pro Ala Ala Phe Val Leu Lys Pro Phe Thr Arg 145 150 155 160 Gln Glu Ala Gly Glu Leu Pro Phe Leu Leu Ser Asp Ala Ala Asp Glu 165 170 175 Leu Glu Arg Ala Leu 180 <210> 93 <211> 1389 <212> DNA <213> Corynbacterium humireducens <220> <221> CDS <222> (301)..(1089) <223> gene lplA <400> 93 gaaggtcggc atcacctccg tggccaccga acgcctcggc gaggtcgtct acgcggagct 60 cccgcaggtc ggtgacgccg tcaccgccgg tgaaccgtgc ggcgaggtcg agtccacgaa 120 gtcggtctcc gacctctacg cgccggtcac gggcaccgtc accgaggtca acgacgcggt 180 ccacgacgac tacgccgtga tcaacgacga ccccttcggc gcgggctggc tcttcgccgt 240 cgaggtcgac gaggtcggac cgctcatgac cgccgccgag tacgcggagg ccaacggcgt 300 gtg acc gcc cgg gac cgg tac cct ggt ccc atc atg act gca ccc cgt 348 Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg 1 5 10 15 gag ccc ttc ttc cct gcg gac cgt tcc atc cgt gcc tcc ggc gag ccc 396 Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro 20 25 30 ccg gag atc cgt tat ctc ggc gtc gtc gac tac cag gag gcc tgg aat 444 Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn 35 40 45 ctg cag gcc gag ctg gcg tcg caa cgc gcg gcc ggg gag atc ggt gac 492 Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp 50 55 60 cag ctc ctc atc ctc gag cac ccg agc gtg tac acc gcc ggc aag cgc 540 Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg 65 70 75 80 acg cag ccg gag gac cgc ccc acc aac ggc ctg ccg gtc atc gac gtc 588 Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val 85 90 95 gac cgg ggt ggc cgc atc acc tgg cac ggc gag gga cag ctg gtg gtc 636 Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val 100 105 110 tac ccg atc atc aaa ctg gcc gac ccg gtg gac gtc gtg gac tat gtc 684 Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val 115 120 125 cgc cgc ctc gag gag gcg gtg atc gcg acg gtg cgg gag gtg ggc gtc 732 Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val 130 135 140 ggc aat gcg ggt cgc atc gac ggc cgc tcc gga gtc tgg gtc ccc gcc 780 Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala 145 150 155 160 gac ggg acc cga cgc gac cgg aag gtg gcg gcg ctc ggc atc cgt gtc 828 Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val 165 170 175 acc cgc ggg gtg acc atg cac ggg ctg agc ctg aac tgc tcg aac acg 876 Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr 180 185 190 ctc gag cac tac ggg cac atc gtg gcg tgc ggc atc gac gac gcc gac 924 Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp 195 200 205 gtc acc acc ctc agc atg gaa ctg ggg cgc gac gtc agc gtc ggg gag 972 Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu 210 215 220 gtc acg gca ccg ctt gtc gac gcc ctc ctg gcc gcc ctc gcc ggt gaa 1020 Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu 225 230 235 240 ctc gcc gtc gcg gac cac acc ttc gga tcg gcg ccc gat ccg acg aag 1068 Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys 245 250 255 att ccc gcg aaa acg cgc tga cctctaagtt tatccctatc agtagaaaga 1119 Ile Pro Ala Lys Thr Arg 260 gtatggtggc gttgtgacta tcgcacctga aggacgcaag ctgctccggg tggagaagcg 1179 caatgcgcag acccccatcg agacgaagcc ccgttggatc cgcaacgcgg tgaagaccgg 1239 ccccgagtac gaggacatga agaagaaggt ggcgggcgcc tccctgcaca ccgtctgtca 1299 ggaggcgggc tgccccaaca tccacgagtg ctgggagtcc cgcgaggcga cgtttctcat 1359 cggcggcgcc aactgctccc gccgctgcga 1389 <210> 94 <211> 262 <212> PRT <213> Corynbacterium humireducens <400> 94 Val Thr Ala Arg Asp Arg Tyr Pro Gly Pro Ile Met Thr Ala Pro Arg 1 5 10 15 Glu Pro Phe Phe Pro Ala Asp Arg Ser Ile Arg Ala Ser Gly Glu Pro 20 25 30 Pro Glu Ile Arg Tyr Leu Gly Val Val Asp Tyr Gln Glu Ala Trp Asn 35 40 45 Leu Gln Ala Glu Leu Ala Ser Gln Arg Ala Ala Gly Glu Ile Gly Asp 50 55 60 Gln Leu Leu Ile Leu Glu His Pro Ser Val Tyr Thr Ala Gly Lys Arg 65 70 75 80 Thr Gln Pro Glu Asp Arg Pro Thr Asn Gly Leu Pro Val Ile Asp Val 85 90 95 Asp Arg Gly Gly Arg Ile Thr Trp His Gly Glu Gly Gln Leu Val Val 100 105 110 Tyr Pro Ile Ile Lys Leu Ala Asp Pro Val Asp Val Val Asp Tyr Val 115 120 125 Arg Arg Leu Glu Glu Ala Val Ile Ala Thr Val Arg Glu Val Gly Val 130 135 140 Gly Asn Ala Gly Arg Ile Asp Gly Arg Ser Gly Val Trp Val Pro Ala 145 150 155 160 Asp Gly Thr Arg Arg Asp Arg Lys Val Ala Ala Leu Gly Ile Arg Val 165 170 175 Thr Arg Gly Val Thr Met His Gly Leu Ser Leu Asn Cys Ser Asn Thr 180 185 190 Leu Glu His Tyr Gly His Ile Val Ala Cys Gly Ile Asp Asp Ala Asp 195 200 205 Val Thr Thr Leu Ser Met Glu Leu Gly Arg Asp Val Ser Val Gly Glu 210 215 220 Val Thr Ala Pro Leu Val Asp Ala Leu Leu Ala Ala Leu Ala Gly Glu 225 230 235 240 Leu Ala Val Ala Asp His Thr Phe Gly Ser Ala Pro Asp Pro Thr Lys 245 250 255 Ile Pro Ala Lys Thr Arg 260 <210> 95 <211> 2010 <212> DNA <213> Corynbacterium humireducens <220> <221> CDS <222> (301)..(1710) <223> gene gcvL <400> 95 caactgacat cacagagaga aggcacacat gtccatcttc gagcagatca tcgtcgccat 60 caacaacttc gtcggcccgg gcatccacgc cctgtcctcc gcttcctcca acgccgcact 120 ggccatcggc ctcttctagg tcgaccccgg aagcatctgc ttatcgacgc cccggccccc 180 ggtcggggcg ttcgtcgttg cggcaccaca gtcccgccac cgtcccaccg cccccacggg 240 ggcgggatcc ttttgtgggg ggcggtgaca cgggggaaac ggtcacctat ccttggtggc 300 gtg act gaa cat tat gac gtt gtt gta ctc ggt gcg ggc ccc ggt ggc 348 Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly 1 5 10 15 tac gtc gcc gcc atc cgc gca gcc cag ctc ggc aag aag gtt gct gtc 396 Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val 20 25 30 gtc gag aag cag tac tgg ggc gga gtc tgc ctc aac gtg ggc tgc atc 444 Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile 35 40 45 ccc tcg aag gcg ctg atc aag aac gcc gag atc gcc cac atc ttc acg 492 Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr 50 55 60 cac gag aag aag acg ttc ggc atc aac ggc gac gtc tcc ttc aac tac 540 His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr 65 70 75 80 gag gac gcc cac aag cgc tcc cgt cag gtg tcg gag aag atc gtc ggc 588 Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly 85 90 95 ggc gtc cac tac ctg atg aag aag aac aag atc acc gag atc gac ggc 636 Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly 100 105 110 ttc gga acc ttc agg gac gcc aag acc atc gag gtc agc gag ggc aag 684 Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys 115 120 125 gac gcc ggc aag gtc gtc acc ttc gac gac tgc atc atc gcc acc ggc 732 Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly 130 135 140 tcc gtg gtc aac agc ctc cgt ggc gtc gag ttc tcc gag aac gtc gtc 780 Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val 145 150 155 160 tcc tac gag gag cag atc ctc aac ccg gtc gcc ccg gag aag atg gtc 828 Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val 165 170 175 atc gtc ggt gcc ggc gcc atc ggc atg gag ttc gcc tac gtg ctc tcc 876 Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser 180 185 190 aac tac ggc gtc gac gtc acc gtc atc gag tac atg gac cgt gtc ctg 924 Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu 195 200 205 ccg aac gag gac gag gac gtc tcc aag gtc atc gcc aag gcc tac aag 972 Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys 210 215 220 aag atg ggc gtc acg ctc ctc ccg ggc cac gcc acc acc gcc gtg cgc 1020 Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg 225 230 235 240 gac aac ggc gac tcc gtc gag gtc gac tac cag aag aag ggc tcg gac 1068 Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp 245 250 255 aag acc gag acc atc gtc gcc gac cgc gtc ctc gtc tcc gtc ggc ttc 1116 Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe 260 265 270 cgc ccg cgt gtc gag ggc ttc ggc ctg gag aac acc ggc gtc gag ctc 1164 Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu 275 280 285 acc gag cgc ggt gcc atc gcc atc gac gac ttc atg cgc acc aac gtc 1212 Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val 290 295 300 gag cac atc tac gcc atc ggt gac gtc acc gcc aag ctg cag ctg gcc 1260 Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala 305 310 315 320 cac gtc gcc gag gcg cag ggc atc gtc gcc gcc gag acc atc gcc ggc 1308 His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly 325 330 335 gcc gag acc cac gag ctg ggc gac tac atg atg atg ccg cgc gcc acc 1356 Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr 340 345 350 ttc tgt aac ccg cag gtc gcg tcc ttc ggc tac acc gag gcg cag gcc 1404 Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala 355 360 365 aag gag aag tgg ccg gac cgc gag atc aag gtc gcg tcc ttc ccg ttc 1452 Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe 370 375 380 tcc gcg aac ggc aag gcc gtc ggc ctg gcc gag acc gag ggc ttc gcc 1500 Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala 385 390 395 400 aag atc gtc gcc gac gcc gag tac ggc gag ctg ctg ggc ggc cac ctg 1548 Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu 405 410 415 gtc ggc gcc aac gcg tcc gag ctg ctc aac gag ctg gtc ctc gcg cag 1596 Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln 420 425 430 aac tac gat ctc acc acc gag gag atc agc cgc gcg gtc cac atc cac 1644 Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His 435 440 445 ccg acc ctg tcg gag gcc gtc aag gag gcc gca cac ggc atc gac ggc 1692 Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly 450 455 460 cac atg atc aac ttc tag tcccccggaa aaaggggcct cccggctggg 1740 His Met Ile Asn Phe 465 aggccccttt tctcatgctt tccgacgccc ccggggcgtc gccaagcgcc tagttgtagg 1800 gcgcgaccga cgaccggtgg gcgtcgatgg tgatctcctc gtggatcggc gggaacgcgc 1860 gctgcgcgca gttctcccgc gggcacacgc ggcagccggc gccgatcggg gtggcgctgg 1920 acaggtcggc gaggttgagt ccctgcgcgt agaccgtgcg gtcggcgtgg cgggcctcgc 1980 agcccaggcc gatggcgaag agcttgccgg 2010 <210> 96 <211> 469 <212> PRT <213> Corynbacterium humireducens <400> 96 Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly 1 5 10 15 Tyr Val Ala Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val 20 25 30 Val Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile 35 40 45 Pro Ser Lys Ala Leu Ile Lys Asn Ala Glu Ile Ala His Ile Phe Thr 50 55 60 His Glu Lys Lys Thr Phe Gly Ile Asn Gly Asp Val Ser Phe Asn Tyr 65 70 75 80 Glu Asp Ala His Lys Arg Ser Arg Gln Val Ser Glu Lys Ile Val Gly 85 90 95 Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Thr Glu Ile Asp Gly 100 105 110 Phe Gly Thr Phe Arg Asp Ala Lys Thr Ile Glu Val Ser Glu Gly Lys 115 120 125 Asp Ala Gly Lys Val Val Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly 130 135 140 Ser Val Val Asn Ser Leu Arg Gly Val Glu Phe Ser Glu Asn Val Val 145 150 155 160 Ser Tyr Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Glu Lys Met Val 165 170 175 Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Ser 180 185 190 Asn Tyr Gly Val Asp Val Thr Val Ile Glu Tyr Met Asp Arg Val Leu 195 200 205 Pro Asn Glu Asp Glu Asp Val Ser Lys Val Ile Ala Lys Ala Tyr Lys 210 215 220 Lys Met Gly Val Thr Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg 225 230 235 240 Asp Asn Gly Asp Ser Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp 245 250 255 Lys Thr Glu Thr Ile Val Ala Asp Arg Val Leu Val Ser Val Gly Phe 260 265 270 Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Glu Leu 275 280 285 Thr Glu Arg Gly Ala Ile Ala Ile Asp Asp Phe Met Arg Thr Asn Val 290 295 300 Glu His Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala 305 310 315 320 His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly 325 330 335 Ala Glu Thr His Glu Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr 340 345 350 Phe Cys Asn Pro Gln Val Ala Ser Phe Gly Tyr Thr Glu Ala Gln Ala 355 360 365 Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe 370 375 380 Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Glu Gly Phe Ala 385 390 395 400 Lys Ile Val Ala Asp Ala Glu Tyr Gly Glu Leu Leu Gly Gly His Leu 405 410 415 Val Gly Ala Asn Ala Ser Glu Leu Leu Asn Glu Leu Val Leu Ala Gln 420 425 430 Asn Tyr Asp Leu Thr Thr Glu Glu Ile Ser Arg Ala Val His Ile His 435 440 445 Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Asp Gly 450 455 460 His Met Ile Asn Phe 465 <210> 97 <211> 2442 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2142) <223> gene ilvB <400> 97 cttgtggtag cagcgcggcg cctgccgtaa cgaccctacc cagtcgtctc gacaagcgcc 60 ctcgacagca cccaccatca gtgctgtacg ggggctttgt ctttagatgg tcgcaggtca 120 cttgtgccga cgacagccac caggccgacc ccttccgcac caagggaaga gtcaccaggt 180 agcgtttcaa ttgcattgtc cttgtgaaat taggagcttg ataacgtggc agcttcgcaa 240 acgcccaccc cggccacggt cgccaagaag tccccgggag ccgagaacgc acccgagcgg 300 atg acc ggc gcc cag gcg atc gtc cgg tcc ctc gag gag ctc ggc acc 348 Met Thr Gly Ala Gln Ala Ile Val Arg Ser Leu Glu Glu Leu Gly Thr 1 5 10 15 gac atc gtc ttc ggc ctg ccc ggc ggt gcc atc ctc ccc ctg tac gac 396 Asp Ile Val Phe Gly Leu Pro Gly Gly Ala Ile Leu Pro Leu Tyr Asp 20 25 30 ccc ctc ttc tcc tcg aag aag ctg cgt cac gtg ctg gtg cgc cac gag 444 Pro Leu Phe Ser Ser Lys Lys Leu Arg His Val Leu Val Arg His Glu 35 40 45 cag ggc tcg ggc cac gct gcc acc ggt tac gca cag gtc acc ggc aag 492 Gln Gly Ser Gly His Ala Ala Thr Gly Tyr Ala Gln Val Thr Gly Lys 50 55 60 gtc ggc gtc tgc atc gcc acc tcc ggc ccg ggt gcc acc aac ctg gtc 540 Val Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val 65 70 75 80 acc ccg ctg gcc gac gcc aac ctg gac tcc gtc ccg atg gtc gcc att 588 Thr Pro Leu Ala Asp Ala Asn Leu Asp Ser Val Pro Met Val Ala Ile 85 90 95 acc ggc cag gtc ggc cgt tcc ctg ctg ggc acc gac gcc ttc cag gag 636 Thr Gly Gln Val Gly Arg Ser Leu Leu Gly Thr Asp Ala Phe Gln Glu 100 105 110 gcc gac atc cgg ggt gtg acc atg ccg atc acg aag cac aac ttc atg 684 Ala Asp Ile Arg Gly Val Thr Met Pro Ile Thr Lys His Asn Phe Met 115 120 125 gtc acc gac ccc aac gac atc gcc cgc acc ctg atg gag gcc ttc cac 732 Val Thr Asp Pro Asn Asp Ile Ala Arg Thr Leu Met Glu Ala Phe His 130 135 140 ctc gct ctg acc ggt cgc ccg ggc ccc gtc ctg gtc gac atc ccg aag 780 Leu Ala Leu Thr Gly Arg Pro Gly Pro Val Leu Val Asp Ile Pro Lys 145 150 155 160 gac atc cag aac gcg gag ttc gac ttc gtc tgg ccg ccg aag atg gat 828 Asp Ile Gln Asn Ala Glu Phe Asp Phe Val Trp Pro Pro Lys Met Asp 165 170 175 ctg ccg ggc tac aag ccg gtc acc acg ccg cac tcc cgc cag atc acc 876 Leu Pro Gly Tyr Lys Pro Val Thr Thr Pro His Ser Arg Gln Ile Thr 180 185 190 cag gcc gtc cag atg atc gcc gag gcc gag cgt ccc tgc ctc tac gtc 924 Gln Ala Val Gln Met Ile Ala Glu Ala Glu Arg Pro Cys Leu Tyr Val 195 200 205 ggc ggc ggc gtc atc aag gcc gac gcc aac aag gag ctg ctg gag ttc 972 Gly Gly Gly Val Ile Lys Ala Asp Ala Asn Lys Glu Leu Leu Glu Phe 210 215 220 gcc gag tac acc ggc gtc ccg gtc gtc acc acc ctc atg gcc ctg ggc 1020 Ala Glu Tyr Thr Gly Val Pro Val Val Thr Thr Leu Met Ala Leu Gly 225 230 235 240 gcg ttc ccg gag tcc cac ccg ctg cac atg ggc atg ccg ggc atg cac 1068 Ala Phe Pro Glu Ser His Pro Leu His Met Gly Met Pro Gly Met His 245 250 255 ggc gcc gtc ccg gcc gtc ggt gcc atg cag ggc gct gac ctg ctc atc 1116 Gly Ala Val Pro Ala Val Gly Ala Met Gln Gly Ala Asp Leu Leu Ile 260 265 270 acc atc ggc gcg cgt ttc gac gac cgc gtc acc ggc aac ctc gac gag 1164 Thr Ile Gly Ala Arg Phe Asp Asp Arg Val Thr Gly Asn Leu Asp Glu 275 280 285 ttc gcc ccg gac gcc cag gtc atc cac gcc gac atc gac ccg gcc gag 1212 Phe Ala Pro Asp Ala Gln Val Ile His Ala Asp Ile Asp Pro Ala Glu 290 295 300 atc ggc aag atc cgc gag gcc gcc gtc ccg atc gtc ggc gac gcc cgc 1260 Ile Gly Lys Ile Arg Glu Ala Ala Val Pro Ile Val Gly Asp Ala Arg 305 310 315 320 gag gtc ctc tcc gca ctc ctg cgc acc tac aag tcc tcc aag aac ctc 1308 Glu Val Leu Ser Ala Leu Leu Arg Thr Tyr Lys Ser Ser Lys Asn Leu 325 330 335 acc ccg ccg aag atc acc gag tgg gtc gag tac ctg acg gac ctc aag 1356 Thr Pro Pro Lys Ile Thr Glu Trp Val Glu Tyr Leu Thr Asp Leu Lys 340 345 350 gag cgc ttc ccg cgc ggc tac gac gag cag tcg gac ggc atg atg agc 1404 Glu Arg Phe Pro Arg Gly Tyr Asp Glu Gln Ser Asp Gly Met Met Ser 355 360 365 ccg cag ttc gtc atc gag acc ctg tcc aag gag gtc ggc ccg gac gcg 1452 Pro Gln Phe Val Ile Glu Thr Leu Ser Lys Glu Val Gly Pro Asp Ala 370 375 380 atc tac gtc gcc ggc gtc ggc cag cac cag atg tgg gcc gcg cag ttc 1500 Ile Tyr Val Ala Gly Val Gly Gln His Gln Met Trp Ala Ala Gln Phe 385 390 395 400 atc gac ttc gag aag ccg cgc acc tgg ctc aac tcc ggt ggt ctg ggc 1548 Ile Asp Phe Glu Lys Pro Arg Thr Trp Leu Asn Ser Gly Gly Leu Gly 405 410 415 acg atg ggc tac tcc atc ccc gcc gcc atg ggc gcc aag gcc ggt ctg 1596 Thr Met Gly Tyr Ser Ile Pro Ala Ala Met Gly Ala Lys Ala Gly Leu 420 425 430 ccg gac gcc gag gtc tgg gcc atc gac ggt gac ggc tgc ttc cag atg 1644 Pro Asp Ala Glu Val Trp Ala Ile Asp Gly Asp Gly Cys Phe Gln Met 435 440 445 acc aac cag gag ctc acc acc gcc gcg gtc gag ggc ttc ccg atc aag 1692 Thr Asn Gln Glu Leu Thr Thr Ala Ala Val Glu Gly Phe Pro Ile Lys 450 455 460 gtc gcc ctg atc aac aac ggc aac ctg ggc atg gtc cgc cag tgg cag 1740 Val Ala Leu Ile Asn Asn Gly Asn Leu Gly Met Val Arg Gln Trp Gln 465 470 475 480 acc ctg ttc tac ggt ggc cgc tac tcg aac acg aag ctg cgt gag cag 1788 Thr Leu Phe Tyr Gly Gly Arg Tyr Ser Asn Thr Lys Leu Arg Glu Gln 485 490 495 gac gag tac gtg ccg gac ttc gtc acg ctg tcc gag gcg ctc ggc tgc 1836 Asp Glu Tyr Val Pro Asp Phe Val Thr Leu Ser Glu Ala Leu Gly Cys 500 505 510 gtc gcc ttc cgc gtc acc cgc gag gag gac gtc ctg ccg acg atc cag 1884 Val Ala Phe Arg Val Thr Arg Glu Glu Asp Val Leu Pro Thr Ile Gln 515 520 525 aag gca cgc gag atc aac gac cgc ccg gtc gtc atc gac ttc atc gtc 1932 Lys Ala Arg Glu Ile Asn Asp Arg Pro Val Val Ile Asp Phe Ile Val 530 535 540 ggt gag gac gcc cag gtc tgg ccg atg gtc gcc gcc ggc gcc tcc aac 1980 Gly Glu Asp Ala Gln Val Trp Pro Met Val Ala Ala Gly Ala Ser Asn 545 550 555 560 tcg gac atc gag tac gca cgt ggt ctg cgc ccc ttc ttc gag ggt gac 2028 Ser Asp Ile Glu Tyr Ala Arg Gly Leu Arg Pro Phe Phe Glu Gly Asp 565 570 575 gag tcc gcg ggt gag tcc ccg gcc gac atc cac ggt gtc atg gag aac 2076 Glu Ser Ala Gly Glu Ser Pro Ala Asp Ile His Gly Val Met Glu Asn 580 585 590 gcc cag gag gag acc gtc gag gaa gtc ctc gag gag cgc gcg aac gac 2124 Ala Gln Glu Glu Thr Val Glu Glu Val Leu Glu Glu Arg Ala Asn Asp 595 600 605 agc gag aag gag aac tag gacaatggcg aacaccgaca tcatccgcaa 2172 Ser Glu Lys Glu Asn 610 caccctcagc gtcctggtcc aggacgtcga cggcatcatc tcccgcgtcg caggcatgtt 2232 cacccgccgt ggctacaacc tggtgtccat cgtctcggcg cacaccgaca ccccgggcat 2292 cagccgcatc accatcgtgg tcgacgccaa cgagctggtc atcgagcagg tcaccaagca 2352 gctcaacaag atcatcccgg tgctcaaggt cgtccgcctc gaggaggaca ccaccatcgc 2412 ccgcgccatc atgctggtga aggtcaacgc 2442 <210> 98 <211> 613 <212> PRT <213> Corynebacterium humireducens <400> 98 Met Thr Gly Ala Gln Ala Ile Val Arg Ser Leu Glu Glu Leu Gly Thr 1 5 10 15 Asp Ile Val Phe Gly Leu Pro Gly Gly Ala Ile Leu Pro Leu Tyr Asp 20 25 30 Pro Leu Phe Ser Ser Lys Lys Leu Arg His Val Leu Val Arg His Glu 35 40 45 Gln Gly Ser Gly His Ala Ala Thr Gly Tyr Ala Gln Val Thr Gly Lys 50 55 60 Val Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val 65 70 75 80 Thr Pro Leu Ala Asp Ala Asn Leu Asp Ser Val Pro Met Val Ala Ile 85 90 95 Thr Gly Gln Val Gly Arg Ser Leu Leu Gly Thr Asp Ala Phe Gln Glu 100 105 110 Ala Asp Ile Arg Gly Val Thr Met Pro Ile Thr Lys His Asn Phe Met 115 120 125 Val Thr Asp Pro Asn Asp Ile Ala Arg Thr Leu Met Glu Ala Phe His 130 135 140 Leu Ala Leu Thr Gly Arg Pro Gly Pro Val Leu Val Asp Ile Pro Lys 145 150 155 160 Asp Ile Gln Asn Ala Glu Phe Asp Phe Val Trp Pro Pro Lys Met Asp 165 170 175 Leu Pro Gly Tyr Lys Pro Val Thr Thr Pro His Ser Arg Gln Ile Thr 180 185 190 Gln Ala Val Gln Met Ile Ala Glu Ala Glu Arg Pro Cys Leu Tyr Val 195 200 205 Gly Gly Gly Val Ile Lys Ala Asp Ala Asn Lys Glu Leu Leu Glu Phe 210 215 220 Ala Glu Tyr Thr Gly Val Pro Val Val Thr Thr Leu Met Ala Leu Gly 225 230 235 240 Ala Phe Pro Glu Ser His Pro Leu His Met Gly Met Pro Gly Met His 245 250 255 Gly Ala Val Pro Ala Val Gly Ala Met Gln Gly Ala Asp Leu Leu Ile 260 265 270 Thr Ile Gly Ala Arg Phe Asp Asp Arg Val Thr Gly Asn Leu Asp Glu 275 280 285 Phe Ala Pro Asp Ala Gln Val Ile His Ala Asp Ile Asp Pro Ala Glu 290 295 300 Ile Gly Lys Ile Arg Glu Ala Ala Val Pro Ile Val Gly Asp Ala Arg 305 310 315 320 Glu Val Leu Ser Ala Leu Leu Arg Thr Tyr Lys Ser Ser Lys Asn Leu 325 330 335 Thr Pro Pro Lys Ile Thr Glu Trp Val Glu Tyr Leu Thr Asp Leu Lys 340 345 350 Glu Arg Phe Pro Arg Gly Tyr Asp Glu Gln Ser Asp Gly Met Met Ser 355 360 365 Pro Gln Phe Val Ile Glu Thr Leu Ser Lys Glu Val Gly Pro Asp Ala 370 375 380 Ile Tyr Val Ala Gly Val Gly Gln His Gln Met Trp Ala Ala Gln Phe 385 390 395 400 Ile Asp Phe Glu Lys Pro Arg Thr Trp Leu Asn Ser Gly Gly Leu Gly 405 410 415 Thr Met Gly Tyr Ser Ile Pro Ala Ala Met Gly Ala Lys Ala Gly Leu 420 425 430 Pro Asp Ala Glu Val Trp Ala Ile Asp Gly Asp Gly Cys Phe Gln Met 435 440 445 Thr Asn Gln Glu Leu Thr Thr Ala Ala Val Glu Gly Phe Pro Ile Lys 450 455 460 Val Ala Leu Ile Asn Asn Gly Asn Leu Gly Met Val Arg Gln Trp Gln 465 470 475 480 Thr Leu Phe Tyr Gly Gly Arg Tyr Ser Asn Thr Lys Leu Arg Glu Gln 485 490 495 Asp Glu Tyr Val Pro Asp Phe Val Thr Leu Ser Glu Ala Leu Gly Cys 500 505 510 Val Ala Phe Arg Val Thr Arg Glu Glu Asp Val Leu Pro Thr Ile Gln 515 520 525 Lys Ala Arg Glu Ile Asn Asp Arg Pro Val Val Ile Asp Phe Ile Val 530 535 540 Gly Glu Asp Ala Gln Val Trp Pro Met Val Ala Ala Gly Ala Ser Asn 545 550 555 560 Ser Asp Ile Glu Tyr Ala Arg Gly Leu Arg Pro Phe Phe Glu Gly Asp 565 570 575 Glu Ser Ala Gly Glu Ser Pro Ala Asp Ile His Gly Val Met Glu Asn 580 585 590 Ala Gln Glu Glu Thr Val Glu Glu Val Leu Glu Glu Arg Ala Asn Asp 595 600 605 Ser Glu Lys Glu Asn 610 <210> 99 <211> 1614 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1314) <223> gene ilvC <400> 99 ccgcgcccgc atcgtcgacg tggcccagga gtccgtggtc atcgaggcca ccggtacccc 60 gggcaagctg cgcgccctgc tggaggtcct cgagcccttc ggtatccgtg agctcatcca 120 gtccggtcag atcgccctca accgcggtcc gaagacgatg gcaccgtcca agtaacaccc 180 cctcatccca cctggtgaga caacacgcat gaaagtccca ccaggtgggg tacccctgtc 240 tcatgttgtg gtacaaacaa caacagcact tgtataagac gaaaggcgag tttttctccc 300 atg gct att gaa ctg ctt tac gac gcc gac gct gac ctc tcc ctc atc 348 Met Ala Ile Glu Leu Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile 1 5 10 15 cag ggc cgc aag gtc gcc atc atc ggc tac ggc tcc cag ggc cac gcc 396 Gln Gly Arg Lys Val Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala 20 25 30 cac gcc cag aac ctg cgt gac tcc ggc gtc gag gtc tgc atc ggc ctg 444 His Ala Gln Asn Leu Arg Asp Ser Gly Val Glu Val Cys Ile Gly Leu 35 40 45 cgc gac ggc tcc aag tcc gcg gag aag gcc aag gag gcc ggc ttc gag 492 Arg Asp Gly Ser Lys Ser Ala Glu Lys Ala Lys Glu Ala Gly Phe Glu 50 55 60 gtc aag tcc aac gcc gac gcc gcc gcc tgg gca gac gtc atc atg ctg 540 Val Lys Ser Asn Ala Asp Ala Ala Ala Trp Ala Asp Val Ile Met Leu 65 70 75 80 ctc gcc ccg gac acc tcc cag aag tcc atc tac gag aac gac atc gag 588 Leu Ala Pro Asp Thr Ser Gln Lys Ser Ile Tyr Glu Asn Asp Ile Glu 85 90 95 ccg aac ctc aac gac ggt gac gcc ctg ctg ttc ggc cac ggc ctg aac 636 Pro Asn Leu Asn Asp Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn 100 105 110 gtc cac ttc ggc ctg atc aag ccg gcc gag aac atc gtc gtc ggc atg 684 Val His Phe Gly Leu Ile Lys Pro Ala Glu Asn Ile Val Val Gly Met 115 120 125 gtc gcc ccg aag ggc ccg ggc cac ctc gtc cgt cgt cag ttc gtc gac 732 Val Ala Pro Lys Gly Pro Gly His Leu Val Arg Arg Gln Phe Val Asp 130 135 140 ggc aag ggc gtc ccc tgc ctc atc gcc gtc gac cag gac ccg aag ggc 780 Gly Lys Gly Val Pro Cys Leu Ile Ala Val Asp Gln Asp Pro Lys Gly 145 150 155 160 aac ggc aag gac ctg ctg ctg tcc tac gcc gcc gcc atc ggt ggt gcc 828 Asn Gly Lys Asp Leu Leu Leu Ser Tyr Ala Ala Ala Ile Gly Gly Ala 165 170 175 cgt gca ggc gtc atc ccg acc acc ttc gag gct gag acc gtc acc gac 876 Arg Ala Gly Val Ile Pro Thr Thr Phe Glu Ala Glu Thr Val Thr Asp 180 185 190 ctg ttc ggt gag cag gcc gtc ctc tgc ggt ggc acc gag gag ctc gtc 924 Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Thr Glu Glu Leu Val 195 200 205 aag acc ggc ttc gag gtt ctg gtc gag gct ggc tac gag ccg gag atg 972 Lys Thr Gly Phe Glu Val Leu Val Glu Ala Gly Tyr Glu Pro Glu Met 210 215 220 gcc tac ttc gag tgc ctc cac gag ctc aag ctg atc gtt gac ctc atg 1020 Ala Tyr Phe Glu Cys Leu His Glu Leu Lys Leu Ile Val Asp Leu Met 225 230 235 240 ttc gag ggc ggc atc gcc aac atg aac tac tcc gtc tcc gac acc gcc 1068 Phe Glu Gly Gly Ile Ala Asn Met Asn Tyr Ser Val Ser Asp Thr Ala 245 250 255 gag ttc ggt ggc tac ctc tcc ggc ccg cgc gtc atc gac gcc ggc acc 1116 Glu Phe Gly Gly Tyr Leu Ser Gly Pro Arg Val Ile Asp Ala Gly Thr 260 265 270 aag gag cgc atg aag gac atc ctg acc gac atc cag gac ggc acc ttc 1164 Lys Glu Arg Met Lys Asp Ile Leu Thr Asp Ile Gln Asp Gly Thr Phe 275 280 285 acc aag cgc ctc atc gcc aac gtc gag aac ggc aac aag gag ctc gag 1212 Thr Lys Arg Leu Ile Ala Asn Val Glu Asn Gly Asn Lys Glu Leu Glu 290 295 300 ggt ctg cgc gag gag tac gcc aag cac cag atc gag gag acc ggc gcc 1260 Gly Leu Arg Glu Glu Tyr Ala Lys His Gln Ile Glu Glu Thr Gly Ala 305 310 315 320 aag ctg cgt gac ctc atg agc tgg gtc aag gtc gag atc acc gag acc 1308 Lys Leu Arg Asp Leu Met Ser Trp Val Lys Val Glu Ile Thr Glu Thr 325 330 335 gcc taa gcccccacgc ttgtcgacgc cccaccggtt cgccggtggg gcgttcgtct 1364 Ala ttgccgggcc agctgagggg cctgcctcag gggtggagca ggtcgagcag gtgccgccgg 1424 agggcggggt cggggtcggc gtcgcgacgc gggcggggga ggtccacggt gatgtcggcg 1484 atgaccgtgg cgtcggggga gggggacatg acgaggatgc ggtcggagag caggatcgcc 1544 tcgtcgacgt cgtgggtgac catgacgacg gtgcggcggt cggactccca cagggagagc 1604 agctccagct 1614 <210> 100 <211> 337 <212> PRT <213> Corynebacterium humireducens <400> 100 Met Ala Ile Glu Leu Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile 1 5 10 15 Gln Gly Arg Lys Val Ala Ile Ile Gly Tyr Gly Ser Gln Gly His Ala 20 25 30 His Ala Gln Asn Leu Arg Asp Ser Gly Val Glu Val Cys Ile Gly Leu 35 40 45 Arg Asp Gly Ser Lys Ser Ala Glu Lys Ala Lys Glu Ala Gly Phe Glu 50 55 60 Val Lys Ser Asn Ala Asp Ala Ala Ala Trp Ala Asp Val Ile Met Leu 65 70 75 80 Leu Ala Pro Asp Thr Ser Gln Lys Ser Ile Tyr Glu Asn Asp Ile Glu 85 90 95 Pro Asn Leu Asn Asp Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn 100 105 110 Val His Phe Gly Leu Ile Lys Pro Ala Glu Asn Ile Val Val Gly Met 115 120 125 Val Ala Pro Lys Gly Pro Gly His Leu Val Arg Arg Gln Phe Val Asp 130 135 140 Gly Lys Gly Val Pro Cys Leu Ile Ala Val Asp Gln Asp Pro Lys Gly 145 150 155 160 Asn Gly Lys Asp Leu Leu Leu Ser Tyr Ala Ala Ala Ile Gly Gly Ala 165 170 175 Arg Ala Gly Val Ile Pro Thr Thr Phe Glu Ala Glu Thr Val Thr Asp 180 185 190 Leu Phe Gly Glu Gln Ala Val Leu Cys Gly Gly Thr Glu Glu Leu Val 195 200 205 Lys Thr Gly Phe Glu Val Leu Val Glu Ala Gly Tyr Glu Pro Glu Met 210 215 220 Ala Tyr Phe Glu Cys Leu His Glu Leu Lys Leu Ile Val Asp Leu Met 225 230 235 240 Phe Glu Gly Gly Ile Ala Asn Met Asn Tyr Ser Val Ser Asp Thr Ala 245 250 255 Glu Phe Gly Gly Tyr Leu Ser Gly Pro Arg Val Ile Asp Ala Gly Thr 260 265 270 Lys Glu Arg Met Lys Asp Ile Leu Thr Asp Ile Gln Asp Gly Thr Phe 275 280 285 Thr Lys Arg Leu Ile Ala Asn Val Glu Asn Gly Asn Lys Glu Leu Glu 290 295 300 Gly Leu Arg Glu Glu Tyr Ala Lys His Gln Ile Glu Glu Thr Gly Ala 305 310 315 320 Lys Leu Arg Asp Leu Met Ser Trp Val Lys Val Glu Ile Thr Glu Thr 325 330 335 Ala <210> 101 <211> 2439 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2139) <223> gene ilvD <400> 101 gaggacgtct ccggcatcgg cttcaagggc tcccgcgccc tgctgaccac ccgcgacggc 60 accgagcacg ccatgcccgg cgtgaccttc aactccctgc cgcagctggc ggaggcctcc 120 cagggccgga tccccgatgt cctcacctcc gcccaggagg cggccgacga caaggtcacc 180 atcatccacc gcgacgggca gcagatcctc atctccaagg aggagtacgc tgctcgacag 240 gccgcgcagc aggccaaccg ctcgtcctct gacaaccctg aaaacctgaa ggagcagtaa 300 gtg ttc ccg ctt cga tcc aaa gtc acc acc gtc gga cgc aac gcc gcc 348 Val Phe Pro Leu Arg Ser Lys Val Thr Thr Val Gly Arg Asn Ala Ala 1 5 10 15 gga gcc cgc gcc ctg tgg cgc gcc acg ggc acc aag gag cac gag ttc 396 Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Thr Lys Glu His Glu Phe 20 25 30 ggt aag ccg atc gtc gcg atc gtg aac tcc tac acc cag ttc gtc ccg 444 Gly Lys Pro Ile Val Ala Ile Val Asn Ser Tyr Thr Gln Phe Val Pro 35 40 45 ggc cac gtc cac ctg aag aac gtg ggc gac atc gtc gcg gac gcc gtc 492 Gly His Val His Leu Lys Asn Val Gly Asp Ile Val Ala Asp Ala Val 50 55 60 cgt gcc gcc ggt ggc gtg ccg aag gag ttc cac acc atc gcc gtc gac 540 Arg Ala Ala Gly Gly Val Pro Lys Glu Phe His Thr Ile Ala Val Asp 65 70 75 80 gac ggc atc gcc atg gga cac ggc ggc atg ctc tac tcg ctg ccc agc 588 Asp Gly Ile Ala Met Gly His Gly Gly Met Leu Tyr Ser Leu Pro Ser 85 90 95 cgt gag atc atc gcc gac tct gtc gag tac atg gtc aac gcc cac acc 636 Arg Glu Ile Ile Ala Asp Ser Val Glu Tyr Met Val Asn Ala His Thr 100 105 110 gcc gac gcg atg gtc tgc atc tcc aac tgc gac aag atc acc ccg ggc 684 Ala Asp Ala Met Val Cys Ile Ser Asn Cys Asp Lys Ile Thr Pro Gly 115 120 125 atg ctc aac gcc gcg ctg cgt ctc aac atc ccg gcc gtg ttc gtc tcc 732 Met Leu Asn Ala Ala Leu Arg Leu Asn Ile Pro Ala Val Phe Val Ser 130 135 140 ggc ggt ccg atg gag gcc ggc aag gcc gtc gtc gtc gac ggc gtc gcc 780 Gly Gly Pro Met Glu Ala Gly Lys Ala Val Val Val Asp Gly Val Ala 145 150 155 160 cag acc ccg acc gac ctc atc acc gcc atc gcc gcc tcc gcc gac gac 828 Gln Thr Pro Thr Asp Leu Ile Thr Ala Ile Ala Ala Ser Ala Asp Asp 165 170 175 aac gtc tcc gat gag ggc ctg gcc acc atc gag gag tcg gcc tgc ccg 876 Asn Val Ser Asp Glu Gly Leu Ala Thr Ile Glu Glu Ser Ala Cys Pro 180 185 190 acc tgt ggc tcc tgc tcc ggc atg ttc acc gcc aac tcg atg aac tgc 924 Thr Cys Gly Ser Cys Ser Gly Met Phe Thr Ala Asn Ser Met Asn Cys 195 200 205 ctc acc gag gcc ctg ggc ctg tcc ctg ccg ggc aac ggc acg acg ctg 972 Leu Thr Glu Ala Leu Gly Leu Ser Leu Pro Gly Asn Gly Thr Thr Leu 210 215 220 gcc acc cac tcc gcc cgc cgt cgt ctc ttc gag cag gcc ggc gag acc 1020 Ala Thr His Ser Ala Arg Arg Arg Leu Phe Glu Gln Ala Gly Glu Thr 225 230 235 240 atc gtc gag ctg tgc agg cgc tac tac ggc gag gag gac gag tcc gtc 1068 Ile Val Glu Leu Cys Arg Arg Tyr Tyr Gly Glu Glu Asp Glu Ser Val 245 250 255 ctg ccg cgc aac atc gcc acc aag gac gcc ttc cgc aac gcg atg gcc 1116 Leu Pro Arg Asn Ile Ala Thr Lys Asp Ala Phe Arg Asn Ala Met Ala 260 265 270 ctc gac atg gcc atg ggc ggc tcc acc aac acg atc ctc cac acg ctg 1164 Leu Asp Met Ala Met Gly Gly Ser Thr Asn Thr Ile Leu His Thr Leu 275 280 285 gcc gcc gcc cag gag ggt gag gtc gac ttc acc ctg gcg gac atc gag 1212 Ala Ala Ala Gln Glu Gly Glu Val Asp Phe Thr Leu Ala Asp Ile Glu 290 295 300 gag atc tcc cac cag gtg ccg tgc atc tcc aag gtc gcc ccg aac ggc 1260 Glu Ile Ser His Gln Val Pro Cys Ile Ser Lys Val Ala Pro Asn Gly 305 310 315 320 atc tac cac atc gag gac gtc cac cgc gcc ggc ggc atc ccg gcc atc 1308 Ile Tyr His Ile Glu Asp Val His Arg Ala Gly Gly Ile Pro Ala Ile 325 330 335 ctc ggc gag ctg cgt cgc gcg aac ctg ctc aac gag gac gtc cac tcc 1356 Leu Gly Glu Leu Arg Arg Ala Asn Leu Leu Asn Glu Asp Val His Ser 340 345 350 atc acc tac gac tcc ctc gac gag tgg ctg ggt gac tgg gac atc cgt 1404 Ile Thr Tyr Asp Ser Leu Asp Glu Trp Leu Gly Asp Trp Asp Ile Arg 355 360 365 ggc ggc cgg gcc atc gac gcc gcg acc gag ctc ttc cac gcc gcc ccg 1452 Gly Gly Arg Ala Ile Asp Ala Ala Thr Glu Leu Phe His Ala Ala Pro 370 375 380 ggt ggc gtg cgc acc acg gag ccc ttc tcc acc gac aac cgc tgg gac 1500 Gly Gly Val Arg Thr Thr Glu Pro Phe Ser Thr Asp Asn Arg Trp Asp 385 390 395 400 agc ctg gac acc gac ccg gtc aac ggc tgc atc cac gac gtc gag cac 1548 Ser Leu Asp Thr Asp Pro Val Asn Gly Cys Ile His Asp Val Glu His 405 410 415 gcc cac tcc gcg gac ggc ggc ctg gtg gtc ctg cgc ggc aac ctg gcg 1596 Ala His Ser Ala Asp Gly Gly Leu Val Val Leu Arg Gly Asn Leu Ala 420 425 430 ccg gac ggc tcc gtc ctc aag acc gcc ggc gtc gag gag gag ctg tgg 1644 Pro Asp Gly Ser Val Leu Lys Thr Ala Gly Val Glu Glu Glu Leu Trp 435 440 445 acc ttc acc ggc ccg gcc cgc gtc gtc gac agc cag gag gag gcc gtc 1692 Thr Phe Thr Gly Pro Ala Arg Val Val Asp Ser Gln Glu Glu Ala Val 450 455 460 tcc atc atc ctc aag cgc gag gtc cag ccg ggt gag gtc gtc gtc atc 1740 Ser Ile Ile Leu Lys Arg Glu Val Gln Pro Gly Glu Val Val Val Ile 465 470 475 480 cgt tac gag ggt cct tcc ggt ggc ccg ggc atg cag gag atg ctg cac 1788 Arg Tyr Glu Gly Pro Ser Gly Gly Pro Gly Met Gln Glu Met Leu His 485 490 495 ccg acg tcc ttc ctc aag ggt gcc ggc ctg ggc aag aag tgc gcc ctc 1836 Pro Thr Ser Phe Leu Lys Gly Ala Gly Leu Gly Lys Lys Cys Ala Leu 500 505 510 atc acc gac ggc cgt ttc tcc ggc ggc acc tcc ggc ttg tcg atc ggc 1884 Ile Thr Asp Gly Arg Phe Ser Gly Gly Thr Ser Gly Leu Ser Ile Gly 515 520 525 cac atc tcc ccg gag gcc gcc cac ggt ggt ctc atc ggc ctg atc cgc 1932 His Ile Ser Pro Glu Ala Ala His Gly Gly Leu Ile Gly Leu Ile Arg 530 535 540 aac ggt gac ccg atc acc atc gac gtc cac acc cgt cgt ctc tcc ctc 1980 Asn Gly Asp Pro Ile Thr Ile Asp Val His Thr Arg Arg Leu Ser Leu 545 550 555 560 gac ctg ccg gac gag gag atc gag gcc cgc cgc gcc gag atg gag gcc 2028 Asp Leu Pro Asp Glu Glu Ile Glu Ala Arg Arg Ala Glu Met Glu Ala 565 570 575 tcc gag aag ccg tgg acc gcg aac cgc gag cgc aag gtc tcc aag gca 2076 Ser Glu Lys Pro Trp Thr Ala Asn Arg Glu Arg Lys Val Ser Lys Ala 580 585 590 ctg cgt gcg tac gcc gcc atg gcc acc tcc gcc gat aag ggc gcc gtc 2124 Leu Arg Ala Tyr Ala Ala Met Ala Thr Ser Ala Asp Lys Gly Ala Val 595 600 605 cgc cag gtg gac taa cattgtccgc gtgaccacga caaacaagcc cgcgatcctg 2179 Arg Gln Val Asp 610 ccccacaccg ccatcctgag gatggtggtg gccctggggc tgatcgcggg cttcggcgtc 2239 accggacgcc agatgatgat caccgacttc cccatcgaca tgatcatcta catggagggc 2299 gtgcgggcct tcctcgacgg cgccgagatg tacgccgtgc cgatgtacgc cggctccctg 2359 gccctgccgt tcatctatcc cccgttcggc gccctcgtca tggtgccgct cacccgttac 2419 gagcatgacc tggccggcga 2439 <210> 102 <211> 612 <212> PRT <213> Corynebacterium humireducens <400> 102 Val Phe Pro Leu Arg Ser Lys Val Thr Thr Val Gly Arg Asn Ala Ala 1 5 10 15 Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Thr Lys Glu His Glu Phe 20 25 30 Gly Lys Pro Ile Val Ala Ile Val Asn Ser Tyr Thr Gln Phe Val Pro 35 40 45 Gly His Val His Leu Lys Asn Val Gly Asp Ile Val Ala Asp Ala Val 50 55 60 Arg Ala Ala Gly Gly Val Pro Lys Glu Phe His Thr Ile Ala Val Asp 65 70 75 80 Asp Gly Ile Ala Met Gly His Gly Gly Met Leu Tyr Ser Leu Pro Ser 85 90 95 Arg Glu Ile Ile Ala Asp Ser Val Glu Tyr Met Val Asn Ala His Thr 100 105 110 Ala Asp Ala Met Val Cys Ile Ser Asn Cys Asp Lys Ile Thr Pro Gly 115 120 125 Met Leu Asn Ala Ala Leu Arg Leu Asn Ile Pro Ala Val Phe Val Ser 130 135 140 Gly Gly Pro Met Glu Ala Gly Lys Ala Val Val Val Asp Gly Val Ala 145 150 155 160 Gln Thr Pro Thr Asp Leu Ile Thr Ala Ile Ala Ala Ser Ala Asp Asp 165 170 175 Asn Val Ser Asp Glu Gly Leu Ala Thr Ile Glu Glu Ser Ala Cys Pro 180 185 190 Thr Cys Gly Ser Cys Ser Gly Met Phe Thr Ala Asn Ser Met Asn Cys 195 200 205 Leu Thr Glu Ala Leu Gly Leu Ser Leu Pro Gly Asn Gly Thr Thr Leu 210 215 220 Ala Thr His Ser Ala Arg Arg Arg Leu Phe Glu Gln Ala Gly Glu Thr 225 230 235 240 Ile Val Glu Leu Cys Arg Arg Tyr Tyr Gly Glu Glu Asp Glu Ser Val 245 250 255 Leu Pro Arg Asn Ile Ala Thr Lys Asp Ala Phe Arg Asn Ala Met Ala 260 265 270 Leu Asp Met Ala Met Gly Gly Ser Thr Asn Thr Ile Leu His Thr Leu 275 280 285 Ala Ala Ala Gln Glu Gly Glu Val Asp Phe Thr Leu Ala Asp Ile Glu 290 295 300 Glu Ile Ser His Gln Val Pro Cys Ile Ser Lys Val Ala Pro Asn Gly 305 310 315 320 Ile Tyr His Ile Glu Asp Val His Arg Ala Gly Gly Ile Pro Ala Ile 325 330 335 Leu Gly Glu Leu Arg Arg Ala Asn Leu Leu Asn Glu Asp Val His Ser 340 345 350 Ile Thr Tyr Asp Ser Leu Asp Glu Trp Leu Gly Asp Trp Asp Ile Arg 355 360 365 Gly Gly Arg Ala Ile Asp Ala Ala Thr Glu Leu Phe His Ala Ala Pro 370 375 380 Gly Gly Val Arg Thr Thr Glu Pro Phe Ser Thr Asp Asn Arg Trp Asp 385 390 395 400 Ser Leu Asp Thr Asp Pro Val Asn Gly Cys Ile His Asp Val Glu His 405 410 415 Ala His Ser Ala Asp Gly Gly Leu Val Val Leu Arg Gly Asn Leu Ala 420 425 430 Pro Asp Gly Ser Val Leu Lys Thr Ala Gly Val Glu Glu Glu Leu Trp 435 440 445 Thr Phe Thr Gly Pro Ala Arg Val Val Asp Ser Gln Glu Glu Ala Val 450 455 460 Ser Ile Ile Leu Lys Arg Glu Val Gln Pro Gly Glu Val Val Val Ile 465 470 475 480 Arg Tyr Glu Gly Pro Ser Gly Gly Pro Gly Met Gln Glu Met Leu His 485 490 495 Pro Thr Ser Phe Leu Lys Gly Ala Gly Leu Gly Lys Lys Cys Ala Leu 500 505 510 Ile Thr Asp Gly Arg Phe Ser Gly Gly Thr Ser Gly Leu Ser Ile Gly 515 520 525 His Ile Ser Pro Glu Ala Ala His Gly Gly Leu Ile Gly Leu Ile Arg 530 535 540 Asn Gly Asp Pro Ile Thr Ile Asp Val His Thr Arg Arg Leu Ser Leu 545 550 555 560 Asp Leu Pro Asp Glu Glu Ile Glu Ala Arg Arg Ala Glu Met Glu Ala 565 570 575 Ser Glu Lys Pro Trp Thr Ala Asn Arg Glu Arg Lys Val Ser Lys Ala 580 585 590 Leu Arg Ala Tyr Ala Ala Met Ala Thr Ser Ala Asp Lys Gly Ala Val 595 600 605 Arg Gln Val Asp 610 <210> 103 <211> 1725 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1425) <223> gene ilvE <400> 103 gatctcgttg gccttgcccg tggcaccgac ggcggtgagg gcgtcgagga cctcgcggga 60 ggcggcgtcg tcgagaagga cggaggccgg cagctccggg ccgtcctcac cgcagaggac 120 ggggacgagc agtgcgtcgg gggtcttcgg ggccttcttg gccagctcga cggcgggggt 180 ggtgccgcgg gcgggaaggg tgaaagtggg gttcgccatg gaaagtacct cctgagattg 240 acaagcgatg aacccagctt atagagatca cctctgggtc gtcggtgtag cgtgttcccc 300 atg acg tct ctc aat ttc acc gtg aac cgt acg agc acc ccg acg tcc 348 Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser 1 5 10 15 gat gag gcg cgc gag gag att ctg cag aat ccg cgg ttc ggc aag aac 396 Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn 20 25 30 ttc acc gat cac atg gtc acc atc gag tgg tcc gag gac aag ggc tgg 444 Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp 35 40 45 cac gac gcc cgc gtg cgt ccc tac gag gcc atc ccg atg gat ccc gcg 492 His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala 50 55 60 acc acg gtg ttc cac tac ggc cag gcg atc ttc gag ggc atc aag gcc 540 Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala 65 70 75 80 tac cgt cag ccg gac ggc tcc atc gcc acc ttc cgc ccg gag cgc aac 588 Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn 85 90 95 gcc gag cgc atg cag cgt tcc gcc gag cgc atg gcc atg ccg ccg ctg 636 Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu 100 105 110 ccg acc gag gac ttc ctc gag acg ctc cgc ctg ctt gtc gac gtc gac 684 Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp 115 120 125 cgt gac tgg gtc ccc gcc gcc ggc ggc gag gcc agc ctc tac ctg cgt 732 Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg 130 135 140 ccc ttc atg atc tcc acc gag gtc tcc ctg ggc gtc agc ccg gcc aac 780 Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn 145 150 155 160 cgg tac acc ttc ttc gtc atc gcc tcc ccg gtc ggg gcg tac ttc acc 828 Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr 165 170 175 ggt ggc gtc aag ccg gtc tcc gtg tgg ctg agc gag gac tac gtc cgt 876 Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg 180 185 190 gcc gcc ccg ggt ggc acc ggt gcc gcg aag ttc gcc ggc aac tac gcc 924 Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala 195 200 205 gcc tcc ctg ctc gcg cag gcg cag gcg gag gag aag ggc tgc gac cag 972 Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln 210 215 220 gtc gtg tgg ctc gac gcc atc gag cac acc tac atc gag gag atg ggt 1020 Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly 225 230 235 240 ggc atg aac ctg ttc ttc gtg tac ggc tcg ggt gac gac gtc acc gtg 1068 Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val 245 250 255 gcc acc ccc gag ctc tcc ggc tcc ctc ctg ccg ggc gtc acc cgc gac 1116 Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp 260 265 270 tcc ctc ctg cag gtc gcc cgc gat ctc ggc cac cgc acc gag gag cgg 1164 Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg 275 280 285 agg atc tcc aag ttc gac tgg cgc gac gac gtc gcc tcc ggt gcg atg 1212 Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met 290 295 300 acc gag gcc atg gcc tgc ggc aca gcc gcc gtg atc acc ccg gtc ggc 1260 Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly 305 310 315 320 cgg gtc ctg tcc aac gac ggc gag ttc cag atc aat gac aac cag ccg 1308 Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro 325 330 335 ggt gag gtc acc atg gcc atg cgc gag cgt ctc acc gac atc cag cgc 1356 Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg 340 345 350 ggc ctg tac gag gac acc cac ggc tgg gtg cac acc ctc gtc ccg gct 1404 Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala 355 360 365 gag gac cag ccg gcg gac tag tcggccagca gctcggcggc ggcccgcacc 1455 Glu Asp Gln Pro Ala Asp 370 agcgggagcg ccgctagcgc gccggtgccc tggccggcgt tcatgtccag ggcgacgaga 1515 ggcgtgagtt cgagggcctg cagcgcgatg aggtgcgcgg gctccggggt cagctgccct 1575 gcctggcacc agggggcggt gccgggggag agacgttccg cgaggtaggc ggcgacggtg 1635 acgggggcgc cgtcgagaag cacgggggtg cggcgtgccg cggcctgcgc gatgaaggcc 1695 accagtgcca ccaggtccgg ggaggcgatc 1725 <210> 104 <211> 374 <212> PRT <213> Corynebacterium humireducens <400> 104 Met Thr Ser Leu Asn Phe Thr Val Asn Arg Thr Ser Thr Pro Thr Ser 1 5 10 15 Asp Glu Ala Arg Glu Glu Ile Leu Gln Asn Pro Arg Phe Gly Lys Asn 20 25 30 Phe Thr Asp His Met Val Thr Ile Glu Trp Ser Glu Asp Lys Gly Trp 35 40 45 His Asp Ala Arg Val Arg Pro Tyr Glu Ala Ile Pro Met Asp Pro Ala 50 55 60 Thr Thr Val Phe His Tyr Gly Gln Ala Ile Phe Glu Gly Ile Lys Ala 65 70 75 80 Tyr Arg Gln Pro Asp Gly Ser Ile Ala Thr Phe Arg Pro Glu Arg Asn 85 90 95 Ala Glu Arg Met Gln Arg Ser Ala Glu Arg Met Ala Met Pro Pro Leu 100 105 110 Pro Thr Glu Asp Phe Leu Glu Thr Leu Arg Leu Leu Val Asp Val Asp 115 120 125 Arg Asp Trp Val Pro Ala Ala Gly Gly Glu Ala Ser Leu Tyr Leu Arg 130 135 140 Pro Phe Met Ile Ser Thr Glu Val Ser Leu Gly Val Ser Pro Ala Asn 145 150 155 160 Arg Tyr Thr Phe Phe Val Ile Ala Ser Pro Val Gly Ala Tyr Phe Thr 165 170 175 Gly Gly Val Lys Pro Val Ser Val Trp Leu Ser Glu Asp Tyr Val Arg 180 185 190 Ala Ala Pro Gly Gly Thr Gly Ala Ala Lys Phe Ala Gly Asn Tyr Ala 195 200 205 Ala Ser Leu Leu Ala Gln Ala Gln Ala Glu Glu Lys Gly Cys Asp Gln 210 215 220 Val Val Trp Leu Asp Ala Ile Glu His Thr Tyr Ile Glu Glu Met Gly 225 230 235 240 Gly Met Asn Leu Phe Phe Val Tyr Gly Ser Gly Asp Asp Val Thr Val 245 250 255 Ala Thr Pro Glu Leu Ser Gly Ser Leu Leu Pro Gly Val Thr Arg Asp 260 265 270 Ser Leu Leu Gln Val Ala Arg Asp Leu Gly His Arg Thr Glu Glu Arg 275 280 285 Arg Ile Ser Lys Phe Asp Trp Arg Asp Asp Val Ala Ser Gly Ala Met 290 295 300 Thr Glu Ala Met Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly 305 310 315 320 Arg Val Leu Ser Asn Asp Gly Glu Phe Gln Ile Asn Asp Asn Gln Pro 325 330 335 Gly Glu Val Thr Met Ala Met Arg Glu Arg Leu Thr Asp Ile Gln Arg 340 345 350 Gly Leu Tyr Glu Asp Thr His Gly Trp Val His Thr Leu Val Pro Ala 355 360 365 Glu Asp Gln Pro Ala Asp 370 <210> 105 <211> 1896 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1596) <223> gene ilvA <400> 105 gccgatcggg gccagcgaga gggccgcacc cggggccgag tggacgacgg ggttgtccac 60 gacagcggcg tgggccgggg cgatgaggga gacactggtg gcgagcgccg cgcacagcgc 120 gacagcggaa cggcgaaggg acacggggag aactcctgtg gtcggtgggg tgcagatcgt 180 gtgacatggt ggaccagtga catgacacca atctcaattc cggaccacct gcaggtgaac 240 agtcggtgaa gtgggctgca acgccgtggc cggtggacag ggggactaca ctgagtcccc 300 atg agt gac acc cgg tct gcc gta acc acc ttc gag cct gtc cgc gcc 348 Met Ser Asp Thr Arg Ser Ala Val Thr Thr Phe Glu Pro Val Arg Ala 1 5 10 15 tcg gac att cag ctg gcg cag gca cgg atc tcc tcc gtc atc gag ccc 396 Ser Asp Ile Gln Leu Ala Gln Ala Arg Ile Ser Ser Val Ile Glu Pro 20 25 30 acc ccg ctc cag tac tgc ccc cgc ctc tcc gag gag acc ggc tgc gag 444 Thr Pro Leu Gln Tyr Cys Pro Arg Leu Ser Glu Glu Thr Gly Cys Glu 35 40 45 gtg tac ctc aaa cgc gag gac ctg cag gac gtg cgc tcc tac aag atc 492 Val Tyr Leu Lys Arg Glu Asp Leu Gln Asp Val Arg Ser Tyr Lys Ile 50 55 60 cgc ggc gcc tac tac gcc atg gcc aac ctc gac gag cag cag cgg gac 540 Arg Gly Ala Tyr Tyr Ala Met Ala Asn Leu Asp Glu Gln Gln Arg Asp 65 70 75 80 gcc ggc atc gtc gcc gcc tcc gcc ggc aac cac gca cag ggt gtc gcc 588 Ala Gly Ile Val Ala Ala Ser Ala Gly Asn His Ala Gln Gly Val Ala 85 90 95 tac gcc tgc cgc acg atg ggc atc cgg ggt cgc atc ttc gtc ccc aac 636 Tyr Ala Cys Arg Thr Met Gly Ile Arg Gly Arg Ile Phe Val Pro Asn 100 105 110 cag acg ccg aag cag aag cgt gac cgc atc atg gtc cac ggc ggt gac 684 Gln Thr Pro Lys Gln Lys Arg Asp Arg Ile Met Val His Gly Gly Asp 115 120 125 atg gtg gag ctg gtc gtc acc ggc aac aac ttc gac gag gcc gcc gag 732 Met Val Glu Leu Val Val Thr Gly Asn Asn Phe Asp Glu Ala Ala Glu 130 135 140 gcc gcg cgt gcc gac gcc gcc gag cgc ggc gcc acc atg atc gag ccc 780 Ala Ala Arg Ala Asp Ala Ala Glu Arg Gly Ala Thr Met Ile Glu Pro 145 150 155 160 ttc gac gcc cgc gac acc gtc atc ggt cag ggg acc atc gcg gcg gag 828 Phe Asp Ala Arg Asp Thr Val Ile Gly Gln Gly Thr Ile Ala Ala Glu 165 170 175 atc ctc acg cag ctc acg tcc atc ggc aag tcc ctc gac acg gtg ctc 876 Ile Leu Thr Gln Leu Thr Ser Ile Gly Lys Ser Leu Asp Thr Val Leu 180 185 190 gtg ccg gtc ggc ggc ggt ggc ctc ctg gcc ggt gtc gcc agc tac ctc 924 Val Pro Val Gly Gly Gly Gly Leu Leu Ala Gly Val Ala Ser Tyr Leu 195 200 205 gcc gac atg gcg ccg cgc acc gcg atc gtc ggc gtc gag ccg ggc ggt 972 Ala Asp Met Ala Pro Arg Thr Ala Ile Val Gly Val Glu Pro Gly Gly 210 215 220 gcc gcc tcc ctc cag gcc gcc ctg cgt gcc gac gcc ccc gtc acc ctc 1020 Ala Ala Ser Leu Gln Ala Ala Leu Arg Ala Asp Ala Pro Val Thr Leu 225 230 235 240 tcc gcg gtc gac ccc ttc gtc gac ggc gcc gcc gtc aag cgc atc ggt 1068 Ser Ala Val Asp Pro Phe Val Asp Gly Ala Ala Val Lys Arg Ile Gly 245 250 255 gac ctg ccc ttc gac atc atc gaa cgc aac aag ggc cgc atc cac gtc 1116 Asp Leu Pro Phe Asp Ile Ile Glu Arg Asn Lys Gly Arg Ile His Val 260 265 270 atg gac gtc tcc gag ggc gcc gtg tgc acc gag atg ctc gac ctg tac 1164 Met Asp Val Ser Glu Gly Ala Val Cys Thr Glu Met Leu Asp Leu Tyr 275 280 285 cag aac gag ggc atc atc acc gag ccc gcc ggt gca ctg tcg gtc acg 1212 Gln Asn Glu Gly Ile Ile Thr Glu Pro Ala Gly Ala Leu Ser Val Thr 290 295 300 ggc ctg aag gag atg aag ctc cag cag gac tcc atc gtg gtg tgc atc 1260 Gly Leu Lys Glu Met Lys Leu Gln Gln Asp Ser Ile Val Val Cys Ile 305 310 315 320 atc tcc ggc ggc aac aac gac gtg ctg cgc tac gcc gag atc atg gag 1308 Ile Ser Gly Gly Asn Asn Asp Val Leu Arg Tyr Ala Glu Ile Met Glu 325 330 335 cgc tcc ctg gtg cac cgc ggc ctc aag cac tac ttc ctg gtg aac ttc 1356 Arg Ser Leu Val His Arg Gly Leu Lys His Tyr Phe Leu Val Asn Phe 340 345 350 ccg cag gag ccg ggc cag ctg cgc cac ttc ctc aac gac atc ctc ggc 1404 Pro Gln Glu Pro Gly Gln Leu Arg His Phe Leu Asn Asp Ile Leu Gly 355 360 365 ccc gac gac gac atc acc ctc ttc gag tac ctc aag cgc aac aac cgg 1452 Pro Asp Asp Asp Ile Thr Leu Phe Glu Tyr Leu Lys Arg Asn Asn Arg 370 375 380 gag acc ggt gcc gcg ctc gtc ggc ctc gag ctc ggc cgg gcc agc gac 1500 Glu Thr Gly Ala Ala Leu Val Gly Leu Glu Leu Gly Arg Ala Ser Asp 385 390 395 400 ctc gac ccg ctg ctc gag cgg atg aac gcc tcc cgc atc gag tgc cag 1548 Leu Asp Pro Leu Leu Glu Arg Met Asn Ala Ser Arg Ile Glu Cys Gln 405 410 415 cac ctc aag ccg aac acc ccc gag tac gag tac ctg gtc aac acc tag 1596 His Leu Lys Pro Asn Thr Pro Glu Tyr Glu Tyr Leu Val Asn Thr 420 425 430 gggtctctac ctgcgcagga gcgcgaactc ccacgggccc aggcgcgtgt cgtcgaggcc 1656 gacctccggt tccgtgaagg agtagatgag ctcgccgccg tagggggcgc ggacctccgc 1716 gtcggagagg ttggccacga ggatcacgtc ctcccagccc atcgccagcc agccgtcccc 1776 ggtctcgacg gtgaggcggg agagatcggc gcgggagagg ccgagctcgc ggcgcagacg 1836 cagcagctcc cggtaggcgg cgtggatctc ctgctgctcc tccgtgaagt cccagtccag 1896 <210> 106 <211> 431 <212> PRT <213> Corynebacterium humireducens <400> 106 Met Ser Asp Thr Arg Ser Ala Val Thr Thr Phe Glu Pro Val Arg Ala 1 5 10 15 Ser Asp Ile Gln Leu Ala Gln Ala Arg Ile Ser Ser Val Ile Glu Pro 20 25 30 Thr Pro Leu Gln Tyr Cys Pro Arg Leu Ser Glu Glu Thr Gly Cys Glu 35 40 45 Val Tyr Leu Lys Arg Glu Asp Leu Gln Asp Val Arg Ser Tyr Lys Ile 50 55 60 Arg Gly Ala Tyr Tyr Ala Met Ala Asn Leu Asp Glu Gln Gln Arg Asp 65 70 75 80 Ala Gly Ile Val Ala Ala Ser Ala Gly Asn His Ala Gln Gly Val Ala 85 90 95 Tyr Ala Cys Arg Thr Met Gly Ile Arg Gly Arg Ile Phe Val Pro Asn 100 105 110 Gln Thr Pro Lys Gln Lys Arg Asp Arg Ile Met Val His Gly Gly Asp 115 120 125 Met Val Glu Leu Val Val Thr Gly Asn Asn Phe Asp Glu Ala Ala Glu 130 135 140 Ala Ala Arg Ala Asp Ala Ala Glu Arg Gly Ala Thr Met Ile Glu Pro 145 150 155 160 Phe Asp Ala Arg Asp Thr Val Ile Gly Gln Gly Thr Ile Ala Ala Glu 165 170 175 Ile Leu Thr Gln Leu Thr Ser Ile Gly Lys Ser Leu Asp Thr Val Leu 180 185 190 Val Pro Val Gly Gly Gly Gly Leu Leu Ala Gly Val Ala Ser Tyr Leu 195 200 205 Ala Asp Met Ala Pro Arg Thr Ala Ile Val Gly Val Glu Pro Gly Gly 210 215 220 Ala Ala Ser Leu Gln Ala Ala Leu Arg Ala Asp Ala Pro Val Thr Leu 225 230 235 240 Ser Ala Val Asp Pro Phe Val Asp Gly Ala Ala Val Lys Arg Ile Gly 245 250 255 Asp Leu Pro Phe Asp Ile Ile Glu Arg Asn Lys Gly Arg Ile His Val 260 265 270 Met Asp Val Ser Glu Gly Ala Val Cys Thr Glu Met Leu Asp Leu Tyr 275 280 285 Gln Asn Glu Gly Ile Ile Thr Glu Pro Ala Gly Ala Leu Ser Val Thr 290 295 300 Gly Leu Lys Glu Met Lys Leu Gln Gln Asp Ser Ile Val Val Cys Ile 305 310 315 320 Ile Ser Gly Gly Asn Asn Asp Val Leu Arg Tyr Ala Glu Ile Met Glu 325 330 335 Arg Ser Leu Val His Arg Gly Leu Lys His Tyr Phe Leu Val Asn Phe 340 345 350 Pro Gln Glu Pro Gly Gln Leu Arg His Phe Leu Asn Asp Ile Leu Gly 355 360 365 Pro Asp Asp Asp Ile Thr Leu Phe Glu Tyr Leu Lys Arg Asn Asn Arg 370 375 380 Glu Thr Gly Ala Ala Leu Val Gly Leu Glu Leu Gly Arg Ala Ser Asp 385 390 395 400 Leu Asp Pro Leu Leu Glu Arg Met Asn Ala Ser Arg Ile Glu Cys Gln 405 410 415 His Leu Lys Pro Asn Thr Pro Glu Tyr Glu Tyr Leu Val Asn Thr 420 425 430 <210> 107 <211> 2043 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1743) <223> gene thrC <400> 107 cggggcagca ccgcgatggc cacgacgagg acgagcagga ccgcggtgac ggtggctacg 60 gtacgtgtgt tacctcggga ggccacgggg cgctcactcc tctgtgcgac tctctgcgac 120 tctgtacgac tctgtgcgac gcggcgtgga caacgccgcc cggcaggggc gtgcggccgc 180 agtaccccgt agcgtactca actgcttttc ccgggaaagg ggaattcacc gggatccccg 240 ccccggcgtg cgggtgggat gttgcgcatg cttccagccc cgccacgtat ggttgtccac 300 gtg gat tac att tcg acg cgc gac tcc tcc cgt act ccc gcc acc ttc 348 Val Asp Tyr Ile Ser Thr Arg Asp Ser Ser Arg Thr Pro Ala Thr Phe 1 5 10 15 acc gac atc ctg ctc ggt gga ctg gcc ccc gac ggc ggc ctc tac ctg 396 Thr Asp Ile Leu Leu Gly Gly Leu Ala Pro Asp Gly Gly Leu Tyr Leu 20 25 30 ccc gcc gag tac ccg cag atc agc gat gag cag ctg acg cag tgg cgg 444 Pro Ala Glu Tyr Pro Gln Ile Ser Asp Glu Gln Leu Thr Gln Trp Arg 35 40 45 tct ctc ctg cag gag aag ggg tac gcc gcc ctg gcg gcg gag atc ctc 492 Ser Leu Leu Gln Glu Lys Gly Tyr Ala Ala Leu Ala Ala Glu Ile Leu 50 55 60 aag ctc ttc gtc gac gac atc ccg gcg gag gat ctc gag ggc atc gcc 540 Lys Leu Phe Val Asp Asp Ile Pro Ala Glu Asp Leu Glu Gly Ile Ala 65 70 75 80 gcc cgc gcc tac acc tcc ccg aag ttc gcg cac gag gag atc gtc ccc 588 Ala Arg Ala Tyr Thr Ser Pro Lys Phe Ala His Glu Glu Ile Val Pro 85 90 95 gtg acc gag ctc gag gac ggc ctg tgg atc ggc cac ctc tcc gag ggc 636 Val Thr Glu Leu Glu Asp Gly Leu Trp Ile Gly His Leu Ser Glu Gly 100 105 110 ccg acc gcc gcg ttc aag gac atg gcg atg cag ctg ctc ggc gag ctc 684 Pro Thr Ala Ala Phe Lys Asp Met Ala Met Gln Leu Leu Gly Glu Leu 115 120 125 ttc gag tac gag ctg gcc cgt cgc ggg gag acc ctc aac atc ctg ggt 732 Phe Glu Tyr Glu Leu Ala Arg Arg Gly Glu Thr Leu Asn Ile Leu Gly 130 135 140 gcg acc tcg ggt gac acg ggt tcc tcc gcc gag tac gcg atg cgc ggc 780 Ala Thr Ser Gly Asp Thr Gly Ser Ser Ala Glu Tyr Ala Met Arg Gly 145 150 155 160 cgt aag ggc atc cgt gtc ttc atg ctg acc ccg gcc ggt cgc atg acc 828 Arg Lys Gly Ile Arg Val Phe Met Leu Thr Pro Ala Gly Arg Met Thr 165 170 175 ccg ttc cag cag gcg cag atg ttc ggc ctg cag gac ccc aac atc ttc 876 Pro Phe Gln Gln Ala Gln Met Phe Gly Leu Gln Asp Pro Asn Ile Phe 180 185 190 aac atc gcc ctg gac ggc gtc ttc gac gac tgc cag gac gtg gtc aag 924 Asn Ile Ala Leu Asp Gly Val Phe Asp Asp Cys Gln Asp Val Val Lys 195 200 205 gcg gtg tcc gcc gac gcc ggg ttc aag gcc gag aac cgt atc ggt gcg 972 Ala Val Ser Ala Asp Ala Gly Phe Lys Ala Glu Asn Arg Ile Gly Ala 210 215 220 gtc aac tcg atc aac tgg gcc cgc ctc atg gcg cag atc gtc tac tac 1020 Val Asn Ser Ile Asn Trp Ala Arg Leu Met Ala Gln Ile Val Tyr Tyr 225 230 235 240 gtc tcc tcc tgg ctg agg atc acc gag agc aac gac cag aag gtc tcc 1068 Val Ser Ser Trp Leu Arg Ile Thr Glu Ser Asn Asp Gln Lys Val Ser 245 250 255 ttc tcc gtg ccg acc ggc aac ttc ggt gac atc tgc gcc ggc cac atc 1116 Phe Ser Val Pro Thr Gly Asn Phe Gly Asp Ile Cys Ala Gly His Ile 260 265 270 gcc cgt cag atg ggt ctg ccg atc gac cgt ctc atc gtc gcc acc aac 1164 Ala Arg Gln Met Gly Leu Pro Ile Asp Arg Leu Ile Val Ala Thr Asn 275 280 285 gag aac gac gtc ctc gac gag ttc ttc cgc acc ggc aac tac cgt ccg 1212 Glu Asn Asp Val Leu Asp Glu Phe Phe Arg Thr Gly Asn Tyr Arg Pro 290 295 300 cgc ccg gcg gag gag acc ctg gag acg tcc tcg ccg tcg atg gac atc 1260 Arg Pro Ala Glu Glu Thr Leu Glu Thr Ser Ser Pro Ser Met Asp Ile 305 310 315 320 tcc cgt gcc tcc aac ttc gag cgt ttc gtc ttc gac ctg ctg ggc cgt 1308 Ser Arg Ala Ser Asn Phe Glu Arg Phe Val Phe Asp Leu Leu Gly Arg 325 330 335 gac gcc gcg cgc gtc gcg cag cac ttc ggc gtc gac gtc cgc gcc gac 1356 Asp Ala Ala Arg Val Ala Gln His Phe Gly Val Asp Val Arg Ala Asp 340 345 350 ggt ttc tcg ctt gcc gac gac ccc gcg ttc ccg gcc gcc gca gag aag 1404 Gly Phe Ser Leu Ala Asp Asp Pro Ala Phe Pro Ala Ala Ala Glu Lys 355 360 365 tac ggc ttc gcc tcc ggt cgt tcc acc cac cag gac cgc ctc gac acg 1452 Tyr Gly Phe Ala Ser Gly Arg Ser Thr His Gln Asp Arg Leu Asp Thr 370 375 380 atc agg gac gtg tgg gag cgc ctg ggc atc atg ctc gac ccg cac acc 1500 Ile Arg Asp Val Trp Glu Arg Leu Gly Ile Met Leu Asp Pro His Thr 385 390 395 400 gcc gac ggc gtg aag gtc gcc cgc gag tgg cgt tcc gag atc gac ggc 1548 Ala Asp Gly Val Lys Val Ala Arg Glu Trp Arg Ser Glu Ile Asp Gly 405 410 415 ccg atc gtg tgc ctg gag acc gcc ctg ccg gtg aag ttc tcc gag acc 1596 Pro Ile Val Cys Leu Glu Thr Ala Leu Pro Val Lys Phe Ser Glu Thr 420 425 430 atc gag gag gcc acc ggc gtg cag ccg gag acc ccg gag cgt ttc gcc 1644 Ile Glu Glu Ala Thr Gly Val Gln Pro Glu Thr Pro Glu Arg Phe Ala 435 440 445 ggc atc ctc gac gcc gag cgc cac gtg acg gac ctg ccg aac gac gcg 1692 Gly Ile Leu Asp Ala Glu Arg His Val Thr Asp Leu Pro Asn Asp Ala 450 455 460 gag acc gtc aag aag ttc atc acc gac tcc atc cgc acc acc gag gtg 1740 Glu Thr Val Lys Lys Phe Ile Thr Asp Ser Ile Arg Thr Thr Glu Val 465 470 475 480 taa ccgtcgtggc catggagttc aagatgggtc aggagtactt ccaggacttc 1793 gacccggaga agttcgccgc gatgatcaag gcgcaggccg aggctgaggc tgacgctgcg 1853 gccggggaag acggcgtcga caagcagtga acatccgcgt ctgggcgggt ctggtcgcac 1913 tgaccaccgc cggcgtgggg tcgctcatcg cctggtccgt cgaggaccag cggcagcacc 1973 ccacgcccat cgccgaggtg atcgcggcgg cgccgacgca ggtcccgacg gagttccgtg 2033 cccctgcggt 2043 <210> 108 <211> 480 <212> PRT <213> Corynebacterium humireducens <400> 108 Val Asp Tyr Ile Ser Thr Arg Asp Ser Ser Arg Thr Pro Ala Thr Phe 1 5 10 15 Thr Asp Ile Leu Leu Gly Gly Leu Ala Pro Asp Gly Gly Leu Tyr Leu 20 25 30 Pro Ala Glu Tyr Pro Gln Ile Ser Asp Glu Gln Leu Thr Gln Trp Arg 35 40 45 Ser Leu Leu Gln Glu Lys Gly Tyr Ala Ala Leu Ala Ala Glu Ile Leu 50 55 60 Lys Leu Phe Val Asp Asp Ile Pro Ala Glu Asp Leu Glu Gly Ile Ala 65 70 75 80 Ala Arg Ala Tyr Thr Ser Pro Lys Phe Ala His Glu Glu Ile Val Pro 85 90 95 Val Thr Glu Leu Glu Asp Gly Leu Trp Ile Gly His Leu Ser Glu Gly 100 105 110 Pro Thr Ala Ala Phe Lys Asp Met Ala Met Gln Leu Leu Gly Glu Leu 115 120 125 Phe Glu Tyr Glu Leu Ala Arg Arg Gly Glu Thr Leu Asn Ile Leu Gly 130 135 140 Ala Thr Ser Gly Asp Thr Gly Ser Ser Ala Glu Tyr Ala Met Arg Gly 145 150 155 160 Arg Lys Gly Ile Arg Val Phe Met Leu Thr Pro Ala Gly Arg Met Thr 165 170 175 Pro Phe Gln Gln Ala Gln Met Phe Gly Leu Gln Asp Pro Asn Ile Phe 180 185 190 Asn Ile Ala Leu Asp Gly Val Phe Asp Asp Cys Gln Asp Val Val Lys 195 200 205 Ala Val Ser Ala Asp Ala Gly Phe Lys Ala Glu Asn Arg Ile Gly Ala 210 215 220 Val Asn Ser Ile Asn Trp Ala Arg Leu Met Ala Gln Ile Val Tyr Tyr 225 230 235 240 Val Ser Ser Trp Leu Arg Ile Thr Glu Ser Asn Asp Gln Lys Val Ser 245 250 255 Phe Ser Val Pro Thr Gly Asn Phe Gly Asp Ile Cys Ala Gly His Ile 260 265 270 Ala Arg Gln Met Gly Leu Pro Ile Asp Arg Leu Ile Val Ala Thr Asn 275 280 285 Glu Asn Asp Val Leu Asp Glu Phe Phe Arg Thr Gly Asn Tyr Arg Pro 290 295 300 Arg Pro Ala Glu Glu Thr Leu Glu Thr Ser Ser Pro Ser Met Asp Ile 305 310 315 320 Ser Arg Ala Ser Asn Phe Glu Arg Phe Val Phe Asp Leu Leu Gly Arg 325 330 335 Asp Ala Ala Arg Val Ala Gln His Phe Gly Val Asp Val Arg Ala Asp 340 345 350 Gly Phe Ser Leu Ala Asp Asp Pro Ala Phe Pro Ala Ala Ala Glu Lys 355 360 365 Tyr Gly Phe Ala Ser Gly Arg Ser Thr His Gln Asp Arg Leu Asp Thr 370 375 380 Ile Arg Asp Val Trp Glu Arg Leu Gly Ile Met Leu Asp Pro His Thr 385 390 395 400 Ala Asp Gly Val Lys Val Ala Arg Glu Trp Arg Ser Glu Ile Asp Gly 405 410 415 Pro Ile Val Cys Leu Glu Thr Ala Leu Pro Val Lys Phe Ser Glu Thr 420 425 430 Ile Glu Glu Ala Thr Gly Val Gln Pro Glu Thr Pro Glu Arg Phe Ala 435 440 445 Gly Ile Leu Asp Ala Glu Arg His Val Thr Asp Leu Pro Asn Asp Ala 450 455 460 Glu Thr Val Lys Lys Phe Ile Thr Asp Ser Ile Arg Thr Thr Glu Val 465 470 475 480 <210> 109 <211> 2448 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2148) <223> gene leuA <400> 109 gggtcgatgc cgtggagcag ggcttttcac gtttcttcac aaaactgcgt tgcgccgggg 60 gagggagtgt gtagacttca cagcatgttc tcccgcgcga acctccttct tcttcgccgc 120 ggcgggtccc aggtccagta acgaagcgac cggcaccccg tcgcggagtt tggtgttgcc 180 ggtcgctgct cccgctgaat agcccattca tcagcggtga gacctcgcag gctactacag 240 tgaaggccgt tgtcggcccc tggtagtccg cagccacaga cagacccgaa aaggaaactc 300 atg tcc ccg aac gac tcc ttc atc tcc gcc ccc tcc gag atc cgc acc 348 Met Ser Pro Asn Asp Ser Phe Ile Ser Ala Pro Ser Glu Ile Arg Thr 1 5 10 15 ccg gac gga ccg cgc cgc gag ggc cag ccc ttc tgg aac aag cag cgc 396 Pro Asp Gly Pro Arg Arg Glu Gly Gln Pro Phe Trp Asn Lys Gln Arg 20 25 30 aac tcc tcc atg ccg gta cac cgc tac cgc ccc ttc gcc gag gcc gtc 444 Asn Ser Ser Met Pro Val His Arg Tyr Arg Pro Phe Ala Glu Ala Val 35 40 45 gag gac atc gtt ctg ccg gac cgc acc tgg ccg gac aag aag atc acc 492 Glu Asp Ile Val Leu Pro Asp Arg Thr Trp Pro Asp Lys Lys Ile Thr 50 55 60 gcc gcc ccg cag tgg tgt gcg gtc gac ctg cgt gac ggc aac cag gcg 540 Ala Ala Pro Gln Trp Cys Ala Val Asp Leu Arg Asp Gly Asn Gln Ala 65 70 75 80 ctc atc gac ccg atg agc ccg gaa cgc aag cgc cgc atg ttc aac ctg 588 Leu Ile Asp Pro Met Ser Pro Glu Arg Lys Arg Arg Met Phe Asn Leu 85 90 95 ctg gtg cag atg ggc tac aag gag atc gag gtt ggt ttc ccg tcc gcc 636 Leu Val Gln Met Gly Tyr Lys Glu Ile Glu Val Gly Phe Pro Ser Ala 100 105 110 tcc cag acc gac ttc gac ttc gtc cgc gag atc atc gag cag gac atg 684 Ser Gln Thr Asp Phe Asp Phe Val Arg Glu Ile Ile Glu Gln Asp Met 115 120 125 atc ccg gag gac gtc acc atc cag gtc ctg gtc cag gcc cgt gag cac 732 Ile Pro Glu Asp Val Thr Ile Gln Val Leu Val Gln Ala Arg Glu His 130 135 140 ctg atc cgc cgc acc ttc gag gcg tgc gag ggc gcg aag aac gtc atc 780 Leu Ile Arg Arg Thr Phe Glu Ala Cys Glu Gly Ala Lys Asn Val Ile 145 150 155 160 gtc cac ttc tac aac tcc acc tcc gag ctg cag cgc cgc gtc gtg ttc 828 Val His Phe Tyr Asn Ser Thr Ser Glu Leu Gln Arg Arg Val Val Phe 165 170 175 cgc aag gat aag ccg gcc atc aag cag ctg gcc gtc gac gcc gcc gag 876 Arg Lys Asp Lys Pro Ala Ile Lys Gln Leu Ala Val Asp Ala Ala Glu 180 185 190 ctg atc cag acc atc gcc cag gac tac ccg ggc acc aac tgg cgt tgg 924 Leu Ile Gln Thr Ile Ala Gln Asp Tyr Pro Gly Thr Asn Trp Arg Trp 195 200 205 cag tac tcc ccg gag tcc ttc acc ggc acc gag ctg gag ttc gcc ctc 972 Gln Tyr Ser Pro Glu Ser Phe Thr Gly Thr Glu Leu Glu Phe Ala Leu 210 215 220 gag gtc tgc aac gcc gtc atc gag gtc atg gcg ccc acc ccg gac aac 1020 Glu Val Cys Asn Ala Val Ile Glu Val Met Ala Pro Thr Pro Asp Asn 225 230 235 240 ccg atc aac atc aac ctg ccg tcc acc gtc gag atg atc acc ccg aac 1068 Pro Ile Asn Ile Asn Leu Pro Ser Thr Val Glu Met Ile Thr Pro Asn 245 250 255 gtc tac gcc gac tcc atc gag tgg atg cac cgc aac ctg gac cgc cgt 1116 Val Tyr Ala Asp Ser Ile Glu Trp Met His Arg Asn Leu Asp Arg Arg 260 265 270 gac tcc gtc atc ctg tcg ctg cac ccc cac aac gac cgt ggt gag ggc 1164 Asp Ser Val Ile Leu Ser Leu His Pro His Asn Asp Arg Gly Glu Gly 275 280 285 gtc gcc gcc gcc gag ctg ggc tac ctg gcc ggc gcc gac cgc atc gag 1212 Val Ala Ala Ala Glu Leu Gly Tyr Leu Ala Gly Ala Asp Arg Ile Glu 290 295 300 ggc tgc ctg ttc ggc aac ggt gag cgc acc ggt aac gtc tgc ctg gtc 1260 Gly Cys Leu Phe Gly Asn Gly Glu Arg Thr Gly Asn Val Cys Leu Val 305 310 315 320 acc ctg ggc ctg aac atg ctc acc cag ggc gtc gac ccg cag atc gac 1308 Thr Leu Gly Leu Asn Met Leu Thr Gln Gly Val Asp Pro Gln Ile Asp 325 330 335 ttc tcc gac atc cgc cag atc cgc agc acc gtc gag tac tgc aac cag 1356 Phe Ser Asp Ile Arg Gln Ile Arg Ser Thr Val Glu Tyr Cys Asn Gln 340 345 350 ctg cga gtc ccg gag cgt cac ccc tac ggc ggc gac ctg gtc ttc acc 1404 Leu Arg Val Pro Glu Arg His Pro Tyr Gly Gly Asp Leu Val Phe Thr 355 360 365 gcc ttc tcc ggc tcc cac cag gac gcc gtc aac aag ggc ctc gac gcc 1452 Ala Phe Ser Gly Ser His Gln Asp Ala Val Asn Lys Gly Leu Asp Ala 370 375 380 atg gcc gcc aag gtc aag ccg ggc gcc aac aac acc gag gtc acc tgg 1500 Met Ala Ala Lys Val Lys Pro Gly Ala Asn Asn Thr Glu Val Thr Trp 385 390 395 400 gag gag ctg ggc aag gcc gtc tgg gag gtt ccc tac ctg ccc atc gac 1548 Glu Glu Leu Gly Lys Ala Val Trp Glu Val Pro Tyr Leu Pro Ile Asp 405 410 415 ccg aag gac gtc ggc cgc gac tac gag gcc gtc atc cgc gtc aac tcc 1596 Pro Lys Asp Val Gly Arg Asp Tyr Glu Ala Val Ile Arg Val Asn Ser 420 425 430 cag tcc ggt aag ggc ggc gtc gcc tac atc atg aag acg gat cac ggc 1644 Gln Ser Gly Lys Gly Gly Val Ala Tyr Ile Met Lys Thr Asp His Gly 435 440 445 atc aac ctg ccg cgt cag atg cag gcc gag ttc tcc tcc gtc gtc cag 1692 Ile Asn Leu Pro Arg Gln Met Gln Ala Glu Phe Ser Ser Val Val Gln 450 455 460 gcg atc acc gac tcc gag ggc gga gag gtc aac tcc aag gag atg tgg 1740 Ala Ile Thr Asp Ser Glu Gly Gly Glu Val Asn Ser Lys Glu Met Trp 465 470 475 480 gac gtc ttc gcc acc gag tac ctg gat gtc acc gag ccg gtg gag gag 1788 Asp Val Phe Ala Thr Glu Tyr Leu Asp Val Thr Glu Pro Val Glu Glu 485 490 495 gtc tcc atc cgc atc gtc aac gcc gag acc gac acc gac gag gcc acc 1836 Val Ser Ile Arg Ile Val Asn Ala Glu Thr Asp Thr Asp Glu Ala Thr 500 505 510 gtc acc gcc acc gtc atc cac aag ggc tcc gag aag gag atc acc ggc 1884 Val Thr Ala Thr Val Ile His Lys Gly Ser Glu Lys Glu Ile Thr Gly 515 520 525 acc ggc aac ggc ccc gtc gcc gcc tac gcc aac gcc ctg gag cag ctg 1932 Thr Gly Asn Gly Pro Val Ala Ala Tyr Ala Asn Ala Leu Glu Gln Leu 530 535 540 ggc atc gac gcg gag gtc cag ttc tac tcc cag cag gcc cgc tcc tcg 1980 Gly Ile Asp Ala Glu Val Gln Phe Tyr Ser Gln Gln Ala Arg Ser Ser 545 550 555 560 ggc gat gac gcc gag gcc gcc tgc tac atc tac gcc acg gtc aac ggc 2028 Gly Asp Asp Ala Glu Ala Ala Cys Tyr Ile Tyr Ala Thr Val Asn Gly 565 570 575 tcc gcc gcc tgg ggc gtc ggt atc gcg ggc tcc atc acc cgc gcc tcc 2076 Ser Ala Ala Trp Gly Val Gly Ile Ala Gly Ser Ile Thr Arg Ala Ser 580 585 590 atc aag gca ctg acc tcc gcg gtc aac cgt gcc atc aag gtc gag gcc 2124 Ile Lys Ala Leu Thr Ser Ala Val Asn Arg Ala Ile Lys Val Glu Ala 595 600 605 tcc gtg ctg gcc ggc ggc gtc tag accgcctgag tcacgggagg ggagtgcgcg 2178 Ser Val Leu Ala Gly Gly Val 610 615 gccgcgcact cccctccttc cgtatctcac cgcccctacc ggcccctacc agcacagtcc 2238 ccgcaggaga ggcactagac tctgaacaat gaccacgacc ccagaccacg gcgattccgc 2298 agctgagacc ccgcctgccg tcgagccggc gttccccttc gtcgctctga cggtccaggc 2358 cacggggatc catccgtcga ccgcccgcct ggtggccgtc gacgtcctga ccttcgacga 2418 ggacggggag atcggcgagg agttccacac 2448 <210> 110 <211> 615 <212> PRT <213> Corynebacterium humireducens <400> 110 Met Ser Pro Asn Asp Ser Phe Ile Ser Ala Pro Ser Glu Ile Arg Thr 1 5 10 15 Pro Asp Gly Pro Arg Arg Glu Gly Gln Pro Phe Trp Asn Lys Gln Arg 20 25 30 Asn Ser Ser Met Pro Val His Arg Tyr Arg Pro Phe Ala Glu Ala Val 35 40 45 Glu Asp Ile Val Leu Pro Asp Arg Thr Trp Pro Asp Lys Lys Ile Thr 50 55 60 Ala Ala Pro Gln Trp Cys Ala Val Asp Leu Arg Asp Gly Asn Gln Ala 65 70 75 80 Leu Ile Asp Pro Met Ser Pro Glu Arg Lys Arg Arg Met Phe Asn Leu 85 90 95 Leu Val Gln Met Gly Tyr Lys Glu Ile Glu Val Gly Phe Pro Ser Ala 100 105 110 Ser Gln Thr Asp Phe Asp Phe Val Arg Glu Ile Ile Glu Gln Asp Met 115 120 125 Ile Pro Glu Asp Val Thr Ile Gln Val Leu Val Gln Ala Arg Glu His 130 135 140 Leu Ile Arg Arg Thr Phe Glu Ala Cys Glu Gly Ala Lys Asn Val Ile 145 150 155 160 Val His Phe Tyr Asn Ser Thr Ser Glu Leu Gln Arg Arg Val Val Phe 165 170 175 Arg Lys Asp Lys Pro Ala Ile Lys Gln Leu Ala Val Asp Ala Ala Glu 180 185 190 Leu Ile Gln Thr Ile Ala Gln Asp Tyr Pro Gly Thr Asn Trp Arg Trp 195 200 205 Gln Tyr Ser Pro Glu Ser Phe Thr Gly Thr Glu Leu Glu Phe Ala Leu 210 215 220 Glu Val Cys Asn Ala Val Ile Glu Val Met Ala Pro Thr Pro Asp Asn 225 230 235 240 Pro Ile Asn Ile Asn Leu Pro Ser Thr Val Glu Met Ile Thr Pro Asn 245 250 255 Val Tyr Ala Asp Ser Ile Glu Trp Met His Arg Asn Leu Asp Arg Arg 260 265 270 Asp Ser Val Ile Leu Ser Leu His Pro His Asn Asp Arg Gly Glu Gly 275 280 285 Val Ala Ala Ala Glu Leu Gly Tyr Leu Ala Gly Ala Asp Arg Ile Glu 290 295 300 Gly Cys Leu Phe Gly Asn Gly Glu Arg Thr Gly Asn Val Cys Leu Val 305 310 315 320 Thr Leu Gly Leu Asn Met Leu Thr Gln Gly Val Asp Pro Gln Ile Asp 325 330 335 Phe Ser Asp Ile Arg Gln Ile Arg Ser Thr Val Glu Tyr Cys Asn Gln 340 345 350 Leu Arg Val Pro Glu Arg His Pro Tyr Gly Gly Asp Leu Val Phe Thr 355 360 365 Ala Phe Ser Gly Ser His Gln Asp Ala Val Asn Lys Gly Leu Asp Ala 370 375 380 Met Ala Ala Lys Val Lys Pro Gly Ala Asn Asn Thr Glu Val Thr Trp 385 390 395 400 Glu Glu Leu Gly Lys Ala Val Trp Glu Val Pro Tyr Leu Pro Ile Asp 405 410 415 Pro Lys Asp Val Gly Arg Asp Tyr Glu Ala Val Ile Arg Val Asn Ser 420 425 430 Gln Ser Gly Lys Gly Gly Val Ala Tyr Ile Met Lys Thr Asp His Gly 435 440 445 Ile Asn Leu Pro Arg Gln Met Gln Ala Glu Phe Ser Ser Val Val Gln 450 455 460 Ala Ile Thr Asp Ser Glu Gly Gly Glu Val Asn Ser Lys Glu Met Trp 465 470 475 480 Asp Val Phe Ala Thr Glu Tyr Leu Asp Val Thr Glu Pro Val Glu Glu 485 490 495 Val Ser Ile Arg Ile Val Asn Ala Glu Thr Asp Thr Asp Glu Ala Thr 500 505 510 Val Thr Ala Thr Val Ile His Lys Gly Ser Glu Lys Glu Ile Thr Gly 515 520 525 Thr Gly Asn Gly Pro Val Ala Ala Tyr Ala Asn Ala Leu Glu Gln Leu 530 535 540 Gly Ile Asp Ala Glu Val Gln Phe Tyr Ser Gln Gln Ala Arg Ser Ser 545 550 555 560 Gly Asp Asp Ala Glu Ala Ala Cys Tyr Ile Tyr Ala Thr Val Asn Gly 565 570 575 Ser Ala Ala Trp Gly Val Gly Ile Ala Gly Ser Ile Thr Arg Ala Ser 580 585 590 Ile Lys Ala Leu Thr Ser Ala Val Asn Arg Ala Ile Lys Val Glu Ala 595 600 605 Ser Val Leu Ala Gly Gly Val 610 615 <210> 111 <211> 1614 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1314) <223> gene leuB <400> 111 ctggtcgtag tactcgtagt catagacgcg gacgtcgtcg ccgagcgcgg ccgccttctc 60 cggggaaccg gtgacggcgg cgacgagctc gacgtcgtcg agactgcgga gcgccgggag 120 gaaggcctgc tgggtgatct ggccgacacc gacgacagcg aaacggacag gggagtcata 180 tgcgtgtgtg ctcatggccc caggctacgg actcgaaacg tgcggcgttg agcgcacgag 240 gggaggggtg ggctgataga atcgccgcca gttcagcagg tgagaaagga atcccacaga 300 atg aaa ctt gca gtc att ggc gga gac ggc atc ggc ccg gag gtc acc 348 Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu Val Thr 1 5 10 15 gaa gag gcg ctc aag gtc ctc cgc acc gtc cgc gac gac atc gag acc 396 Glu Glu Ala Leu Lys Val Leu Arg Thr Val Arg Asp Asp Ile Glu Thr 20 25 30 acc gac tac gat ctc ggc gca cgt cgc tac ctg cgc aac ggt gag ctg 444 Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Arg Asn Gly Glu Leu 35 40 45 ctc acc gac gcc gac ctc gac tcc ctc cgc gag cac gac gcc atc ctc 492 Leu Thr Asp Ala Asp Leu Asp Ser Leu Arg Glu His Asp Ala Ile Leu 50 55 60 ctc ggc gcc atc ggc gcc ccg gac acc gtc gcg ccg ggc gtc ctc gag 540 Leu Gly Ala Ile Gly Ala Pro Asp Thr Val Ala Pro Gly Val Leu Glu 65 70 75 80 cgt ggc ctg ctg ctc aag atg cgt ttc gcc ctc gac cac cac gtc aac 588 Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His Val Asn 85 90 95 ctg cgt ccc tcc aag ctc tac gag ggc gtc gag tcc ccg ctg aag aac 636 Leu Arg Pro Ser Lys Leu Tyr Glu Gly Val Glu Ser Pro Leu Lys Asn 100 105 110 ccg ggc gag atc gac ttc gtc gtc gtc cgc gag ggc acc gag ggc gcc 684 Pro Gly Glu Ile Asp Phe Val Val Val Arg Glu Gly Thr Glu Gly Ala 115 120 125 tac acc ggc aac ggc ggc gcc atc cgc gtg ggc acc ccg cac gag gtc 732 Tyr Thr Gly Asn Gly Gly Ala Ile Arg Val Gly Thr Pro His Glu Val 130 135 140 gcc aac gag acc tcc gtc aac acc cgc tac ggc gcc gag cgc gtc gtc 780 Ala Asn Glu Thr Ser Val Asn Thr Arg Tyr Gly Ala Glu Arg Val Val 145 150 155 160 cgc tac gcc ttc gag ctg gcc atg acc cgc cgc aag cac gtc acc ctg 828 Arg Tyr Ala Phe Glu Leu Ala Met Thr Arg Arg Lys His Val Thr Leu 165 170 175 gtc cac aag acc aac gtg ctc gtc cac ggc ggc ggc atg tgg cag cgc 876 Val His Lys Thr Asn Val Leu Val His Gly Gly Gly Met Trp Gln Arg 180 185 190 acc gtc gac gag gtc gcc acg gag tac cct gag gtc acc gtc gac tac 924 Thr Val Asp Glu Val Ala Thr Glu Tyr Pro Glu Val Thr Val Asp Tyr 195 200 205 gcg cac atc gac gcc gcg acg atc tac atg atc acc gac ccc tcc cgc 972 Ala His Ile Asp Ala Ala Thr Ile Tyr Met Ile Thr Asp Pro Ser Arg 210 215 220 ttc gac gtc atc gtc acc gac aac ctc ttc ggc gac atc atc acc gat 1020 Phe Asp Val Ile Val Thr Asp Asn Leu Phe Gly Asp Ile Ile Thr Asp 225 230 235 240 gag gcc ggt gcc gtc tcc ggt ggc atc ggc ctc gcc gcc tcc ggc aac 1068 Glu Ala Gly Ala Val Ser Gly Gly Ile Gly Leu Ala Ala Ser Gly Asn 245 250 255 atc gac gcc acc cgc acc aac ccg tcc atg ttc gag ccg gtc cac ggc 1116 Ile Asp Ala Thr Arg Thr Asn Pro Ser Met Phe Glu Pro Val His Gly 260 265 270 tcc gca ccg gac atc gcc ggc cag ggc atc gcc gac ccg acc gcc gcc 1164 Ser Ala Pro Asp Ile Ala Gly Gln Gly Ile Ala Asp Pro Thr Ala Ala 275 280 285 atc ctc tcc gcc gcg atg ctg ctg cgt cac ctc ggt gac gag gac aac 1212 Ile Leu Ser Ala Ala Met Leu Leu Arg His Leu Gly Asp Glu Asp Asn 290 295 300 gcc gca cgg atc gag gcc gcg gtg gcc gag gac gtc gcc aag cgt gag 1260 Ala Ala Arg Ile Glu Ala Ala Val Ala Glu Asp Val Ala Lys Arg Glu 305 310 315 320 ggg cag atc cgc acc acc gag gtc ggc gac cgc atc gcc gcc gcc ctc 1308 Gly Gln Ile Arg Thr Thr Glu Val Gly Asp Arg Ile Ala Ala Ala Leu 325 330 335 aag tag tctgtcggca gcagacagac gccatatata taaggagcat tcgtgaggcg 1364 Lys acgtacagca ctactggccc tggccaccac cctggccatg gtcaccggat gctccacctc 1424 ggcggacacc ggtgacgggg acgaccggaa gcccggcacc cccgccctgg agatgacctc 1484 ctccgaggtc atcgccgacg ccgccggcaa cggcgtcgac acctccgccc gcctcttcga 1544 ggaggcggag accgtcgtgg tcgccgccga cacccctgag gaccaggccc ggggcgccca 1604 cctggcgatc 1614 <210> 112 <211> 337 <212> PRT <213> Corynebacterium humireducens <400> 112 Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu Val Thr 1 5 10 15 Glu Glu Ala Leu Lys Val Leu Arg Thr Val Arg Asp Asp Ile Glu Thr 20 25 30 Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Arg Asn Gly Glu Leu 35 40 45 Leu Thr Asp Ala Asp Leu Asp Ser Leu Arg Glu His Asp Ala Ile Leu 50 55 60 Leu Gly Ala Ile Gly Ala Pro Asp Thr Val Ala Pro Gly Val Leu Glu 65 70 75 80 Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His Val Asn 85 90 95 Leu Arg Pro Ser Lys Leu Tyr Glu Gly Val Glu Ser Pro Leu Lys Asn 100 105 110 Pro Gly Glu Ile Asp Phe Val Val Val Arg Glu Gly Thr Glu Gly Ala 115 120 125 Tyr Thr Gly Asn Gly Gly Ala Ile Arg Val Gly Thr Pro His Glu Val 130 135 140 Ala Asn Glu Thr Ser Val Asn Thr Arg Tyr Gly Ala Glu Arg Val Val 145 150 155 160 Arg Tyr Ala Phe Glu Leu Ala Met Thr Arg Arg Lys His Val Thr Leu 165 170 175 Val His Lys Thr Asn Val Leu Val His Gly Gly Gly Met Trp Gln Arg 180 185 190 Thr Val Asp Glu Val Ala Thr Glu Tyr Pro Glu Val Thr Val Asp Tyr 195 200 205 Ala His Ile Asp Ala Ala Thr Ile Tyr Met Ile Thr Asp Pro Ser Arg 210 215 220 Phe Asp Val Ile Val Thr Asp Asn Leu Phe Gly Asp Ile Ile Thr Asp 225 230 235 240 Glu Ala Gly Ala Val Ser Gly Gly Ile Gly Leu Ala Ala Ser Gly Asn 245 250 255 Ile Asp Ala Thr Arg Thr Asn Pro Ser Met Phe Glu Pro Val His Gly 260 265 270 Ser Ala Pro Asp Ile Ala Gly Gln Gly Ile Ala Asp Pro Thr Ala Ala 275 280 285 Ile Leu Ser Ala Ala Met Leu Leu Arg His Leu Gly Asp Glu Asp Asn 290 295 300 Ala Ala Arg Ile Glu Ala Ala Val Ala Glu Asp Val Ala Lys Arg Glu 305 310 315 320 Gly Gln Ile Arg Thr Thr Glu Val Gly Asp Arg Ile Ala Ala Ala Leu 325 330 335 Lys <210> 113 <211> 2019 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1719) <223> gene leuC <400> 113 gctggtcccg gccgccggtg ccgagggcgg tgagtgcggt gccggtggtc cagcggccgt 60 cgtcgccgcg ggcgaggatg cggtgtgtct cgagggcggt ggcgaggcgg tgggcggtgg 120 cgcggggcag gccggtggtc tcgcacagtt cggtgaggga gaggggatgg ggggcgacgg 180 ccatcatgat ggcgacggcc cgatcgagca ccttgatccc tgacactgcg ctatactgtc 240 ccataccacg atactaacat ctcaccttgt gagatttcac agatggaaag gtggacatcc 300 atg gcc gag aag ctc acg ctg gcc gag aag gtg tgg aac gac cat att 348 Met Ala Glu Lys Leu Thr Leu Ala Glu Lys Val Trp Asn Asp His Ile 1 5 10 15 gtc gcg aag ggc gag aac ggc gag ccc gat ctc ctc tac att gac ctg 396 Val Ala Lys Gly Glu Asn Gly Glu Pro Asp Leu Leu Tyr Ile Asp Leu 20 25 30 cag ctc ctg cac gag gtg acc tcc ccg cag gcc ttc gac gga ctg cgc 444 Gln Leu Leu His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg 35 40 45 ctc gcc ggc cgc aag ctc cgc cgc ccg gac ctg cac ctg gcc acc gag 492 Leu Ala Gly Arg Lys Leu Arg Arg Pro Asp Leu His Leu Ala Thr Glu 50 55 60 gac cac aac gtg ccc acc gag ggc atc gtc tcc ggc tcc ctc ctg gag 540 Asp His Asn Val Pro Thr Glu Gly Ile Val Ser Gly Ser Leu Leu Glu 65 70 75 80 atc aag gag gcc acc tcc cgc acg cag gtc gag acc ctg cgc aag aac 588 Ile Lys Glu Ala Thr Ser Arg Thr Gln Val Glu Thr Leu Arg Lys Asn 85 90 95 tgc gag gag ttc ggc atc cgc ctg cac ccg atg ggc gac gtc aag cag 636 Cys Glu Glu Phe Gly Ile Arg Leu His Pro Met Gly Asp Val Lys Gln 100 105 110 ggc atc gtc cac acc gtc ggc ccg cag ctg ggc gcc acc cag ccg ggc 684 Gly Ile Val His Thr Val Gly Pro Gln Leu Gly Ala Thr Gln Pro Gly 115 120 125 atg acc atc gtc tgc ggc gac tcc cac acc tcc acc cac ggt gcc ttc 732 Met Thr Ile Val Cys Gly Asp Ser His Thr Ser Thr His Gly Ala Phe 130 135 140 ggc tcc atc gcc atg ggc atc ggc acc tcc gag gtc gag cac gtc atg 780 Gly Ser Ile Ala Met Gly Ile Gly Thr Ser Glu Val Glu His Val Met 145 150 155 160 gcg acc cag acc ctg ccg ctc aag ccg ttc aag acc atg gcc atc gag 828 Ala Thr Gln Thr Leu Pro Leu Lys Pro Phe Lys Thr Met Ala Ile Glu 165 170 175 gtc tcc ggc gag ctg cag gag ggc gtc acc gcc aag gac ctc atc ttg 876 Val Ser Gly Glu Leu Gln Glu Gly Val Thr Ala Lys Asp Leu Ile Leu 180 185 190 gcg atc atc gcc aag atc ggc acc ggc ggc ggc cag ggc cac atc atc 924 Ala Ile Ile Ala Lys Ile Gly Thr Gly Gly Gly Gln Gly His Ile Ile 195 200 205 gag tac cgc ggc gag gcc atc gag aag ctc tcg atg gag gcc cgg atg 972 Glu Tyr Arg Gly Glu Ala Ile Glu Lys Leu Ser Met Glu Ala Arg Met 210 215 220 acc atc tgc aac atg tcc atc gag gcc ggt gcc cgt gcc ggc atg gtc 1020 Thr Ile Cys Asn Met Ser Ile Glu Ala Gly Ala Arg Ala Gly Met Val 225 230 235 240 gcc ccc gac cag aag acc ttc gac tac gtc cag ggc cgc gag ttc gcc 1068 Ala Pro Asp Gln Lys Thr Phe Asp Tyr Val Gln Gly Arg Glu Phe Ala 245 250 255 ccc acc ggc gag gac tgg gac gcc gcc gtc gcc tac tgg aag acc ctg 1116 Pro Thr Gly Glu Asp Trp Asp Ala Ala Val Ala Tyr Trp Lys Thr Leu 260 265 270 ccg acc gac gag ggc gcc gag ttc gac acc gtc gtc gag atc gac ggc 1164 Pro Thr Asp Glu Gly Ala Glu Phe Asp Thr Val Val Glu Ile Asp Gly 275 280 285 tcc gcg ctg acc ccg ttc gtc acc tgg ggc acc aac ccg ggc cag ggc 1212 Ser Ala Leu Thr Pro Phe Val Thr Trp Gly Thr Asn Pro Gly Gln Gly 290 295 300 ctg ccc ctg tcc gcg aac gtc ccg gac ccg gag gac ttc acc aac gag 1260 Leu Pro Leu Ser Ala Asn Val Pro Asp Pro Glu Asp Phe Thr Asn Glu 305 310 315 320 gcc gac aag gcc gcc gcg gag aag gcc ctg gcc tac atg gac ctc acc 1308 Ala Asp Lys Ala Ala Ala Glu Lys Ala Leu Ala Tyr Met Asp Leu Thr 325 330 335 ccg ggc acc ccg ctg cgg gac atc aag atc gac acc gtc ttc ctc ggc 1356 Pro Gly Thr Pro Leu Arg Asp Ile Lys Ile Asp Thr Val Phe Leu Gly 340 345 350 tcc tgc acc aac gcc cgc atc gag gac ctg cgc gct gcc gcc gag gtc 1404 Ser Cys Thr Asn Ala Arg Ile Glu Asp Leu Arg Ala Ala Ala Glu Val 355 360 365 gtc gag ggc cgc acg atc gcc ccg aac acc cgc atg ctc gtc gtc ccg 1452 Val Glu Gly Arg Thr Ile Ala Pro Asn Thr Arg Met Leu Val Val Pro 370 375 380 tcc tcg acg ctg gtc aag cag cag gcc gag gag gag ggg ctg gac aag 1500 Ser Ser Thr Leu Val Lys Gln Gln Ala Glu Glu Glu Gly Leu Asp Lys 385 390 395 400 atc ttc acc gac ttc ggc gcc gag tgg cgc acc gcc ggc tgc tcc atg 1548 Ile Phe Thr Asp Phe Gly Ala Glu Trp Arg Thr Ala Gly Cys Ser Met 405 410 415 tgc ctg ggc atg aac ccg gac cag ctg gcc ccg ggc gag cgc tcc gcc 1596 Cys Leu Gly Met Asn Pro Asp Gln Leu Ala Pro Gly Glu Arg Ser Ala 420 425 430 tcc acc tcg aac cgt aac ttc gag ggc cgg cag ggc ccg ggc ggc cgc 1644 Ser Thr Ser Asn Arg Asn Phe Glu Gly Arg Gln Gly Pro Gly Gly Arg 435 440 445 acc cac ctg gtg tcc ccg ccg gtc gcc gcc gcc acc gcc gtc ctc ggc 1692 Thr His Leu Val Ser Pro Pro Val Ala Ala Ala Thr Ala Val Leu Gly 450 455 460 cac ctg gct tcc ccc gca gac ctc tga ccacgccgac caccaaggag 1739 His Leu Ala Ser Pro Ala Asp Leu 465 470 ataaaaacag tggagaagtt caccacccac accggcgtcg gcgtcccgct gacccgctcg 1799 aacgtcgaca ccgaccagat catccccgcc gtctacctca agcgcgtcac ccgcacgggc 1859 ttcgatgacg gcctgttcgc cgagtggcgc aaggacgatt ccttcgtcct caaccaggag 1919 ccctaccgca acggctccgt cctggtcgcc ggccccgact tcggcaccgg ctcctcccgt 1979 gagcacgccg tctgggccct catggactac ggcttccgcg 2019 <210> 114 <211> 472 <212> PRT <213> Corynebacterium humireducens <400> 114 Met Ala Glu Lys Leu Thr Leu Ala Glu Lys Val Trp Asn Asp His Ile 1 5 10 15 Val Ala Lys Gly Glu Asn Gly Glu Pro Asp Leu Leu Tyr Ile Asp Leu 20 25 30 Gln Leu Leu His Glu Val Thr Ser Pro Gln Ala Phe Asp Gly Leu Arg 35 40 45 Leu Ala Gly Arg Lys Leu Arg Arg Pro Asp Leu His Leu Ala Thr Glu 50 55 60 Asp His Asn Val Pro Thr Glu Gly Ile Val Ser Gly Ser Leu Leu Glu 65 70 75 80 Ile Lys Glu Ala Thr Ser Arg Thr Gln Val Glu Thr Leu Arg Lys Asn 85 90 95 Cys Glu Glu Phe Gly Ile Arg Leu His Pro Met Gly Asp Val Lys Gln 100 105 110 Gly Ile Val His Thr Val Gly Pro Gln Leu Gly Ala Thr Gln Pro Gly 115 120 125 Met Thr Ile Val Cys Gly Asp Ser His Thr Ser Thr His Gly Ala Phe 130 135 140 Gly Ser Ile Ala Met Gly Ile Gly Thr Ser Glu Val Glu His Val Met 145 150 155 160 Ala Thr Gln Thr Leu Pro Leu Lys Pro Phe Lys Thr Met Ala Ile Glu 165 170 175 Val Ser Gly Glu Leu Gln Glu Gly Val Thr Ala Lys Asp Leu Ile Leu 180 185 190 Ala Ile Ile Ala Lys Ile Gly Thr Gly Gly Gly Gln Gly His Ile Ile 195 200 205 Glu Tyr Arg Gly Glu Ala Ile Glu Lys Leu Ser Met Glu Ala Arg Met 210 215 220 Thr Ile Cys Asn Met Ser Ile Glu Ala Gly Ala Arg Ala Gly Met Val 225 230 235 240 Ala Pro Asp Gln Lys Thr Phe Asp Tyr Val Gln Gly Arg Glu Phe Ala 245 250 255 Pro Thr Gly Glu Asp Trp Asp Ala Ala Val Ala Tyr Trp Lys Thr Leu 260 265 270 Pro Thr Asp Glu Gly Ala Glu Phe Asp Thr Val Val Glu Ile Asp Gly 275 280 285 Ser Ala Leu Thr Pro Phe Val Thr Trp Gly Thr Asn Pro Gly Gln Gly 290 295 300 Leu Pro Leu Ser Ala Asn Val Pro Asp Pro Glu Asp Phe Thr Asn Glu 305 310 315 320 Ala Asp Lys Ala Ala Ala Glu Lys Ala Leu Ala Tyr Met Asp Leu Thr 325 330 335 Pro Gly Thr Pro Leu Arg Asp Ile Lys Ile Asp Thr Val Phe Leu Gly 340 345 350 Ser Cys Thr Asn Ala Arg Ile Glu Asp Leu Arg Ala Ala Ala Glu Val 355 360 365 Val Glu Gly Arg Thr Ile Ala Pro Asn Thr Arg Met Leu Val Val Pro 370 375 380 Ser Ser Thr Leu Val Lys Gln Gln Ala Glu Glu Glu Gly Leu Asp Lys 385 390 395 400 Ile Phe Thr Asp Phe Gly Ala Glu Trp Arg Thr Ala Gly Cys Ser Met 405 410 415 Cys Leu Gly Met Asn Pro Asp Gln Leu Ala Pro Gly Glu Arg Ser Ala 420 425 430 Ser Thr Ser Asn Arg Asn Phe Glu Gly Arg Gln Gly Pro Gly Gly Arg 435 440 445 Thr His Leu Val Ser Pro Pro Val Ala Ala Ala Thr Ala Val Leu Gly 450 455 460 His Leu Ala Ser Pro Ala Asp Leu 465 470 <210> 115 <211> 1197 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(897) <223> gene leuD <400> 115 cccgtcctcg acgctggtca agcagcaggc cgaggaggag gggctggaca agatcttcac 60 cgacttcggc gccgagtggc gcaccgccgg ctgctccatg tgcctgggca tgaacccgga 120 ccagctggcc ccgggcgagc gctccgcctc cacctcgaac cgtaacttcg agggccggca 180 gggcccgggc ggccgcaccc acctggtgtc cccgccggtc gccgccgcca ccgccgtcct 240 cggccacctg gcttcccccg cagacctctg accacgccga ccaccaagga gataaaaaca 300 gtg gag aag ttc acc acc cac acc ggc gtc ggc gtc ccg ctg acc cgc 348 Val Glu Lys Phe Thr Thr His Thr Gly Val Gly Val Pro Leu Thr Arg 1 5 10 15 tcg aac gtc gac acc gac cag atc atc ccc gcc gtc tac ctc aag cgc 396 Ser Asn Val Asp Thr Asp Gln Ile Ile Pro Ala Val Tyr Leu Lys Arg 20 25 30 gtc acc cgc acg ggc ttc gat gac ggc ctg ttc gcc gag tgg cgc aag 444 Val Thr Arg Thr Gly Phe Asp Asp Gly Leu Phe Ala Glu Trp Arg Lys 35 40 45 gac gat tcc ttc gtc ctc aac cag gag ccc tac cgc aac ggc tcc gtc 492 Asp Asp Ser Phe Val Leu Asn Gln Glu Pro Tyr Arg Asn Gly Ser Val 50 55 60 ctg gtc gcc ggc ccc gac ttc ggc acc ggc tcc tcc cgt gag cac gcc 540 Leu Val Ala Gly Pro Asp Phe Gly Thr Gly Ser Ser Arg Glu His Ala 65 70 75 80 gtc tgg gcc ctc atg gac tac ggc ttc cgc gtc gtg ctg tcc tcc cgc 588 Val Trp Ala Leu Met Asp Tyr Gly Phe Arg Val Val Leu Ser Ser Arg 85 90 95 ttc gcc gac atc ttc cgc ggc aac tcc ggc aag gcc ggc ctg ctg gcc 636 Phe Ala Asp Ile Phe Arg Gly Asn Ser Gly Lys Ala Gly Leu Leu Ala 100 105 110 gcc cag atg gag gag gcc gat atc gag ctg ctg tgg aag cag ctc gag 684 Ala Gln Met Glu Glu Ala Asp Ile Glu Leu Leu Trp Lys Gln Leu Glu 115 120 125 gct gag ccg ggc ctg gag atc acc gtc gac ctc gag tcc cgc acc gtc 732 Ala Glu Pro Gly Leu Glu Ile Thr Val Asp Leu Glu Ser Arg Thr Val 130 135 140 acc gcc ggc gag aac acc tac cag ttc tcc gtc gac gac tac acc cgc 780 Thr Ala Gly Glu Asn Thr Tyr Gln Phe Ser Val Asp Asp Tyr Thr Arg 145 150 155 160 tgg cgc ctc atg gag ggc ctc gac gac atc ggc ctg acc ctg cgc gac 828 Trp Arg Leu Met Glu Gly Leu Asp Asp Ile Gly Leu Thr Leu Arg Asp 165 170 175 gag cag gcc atc acc gac tac gag gcg aag cgc ccc gtg tac aag ccg 876 Glu Gln Ala Ile Thr Asp Tyr Glu Ala Lys Arg Pro Val Tyr Lys Pro 180 185 190 acc atc acc gcg gag gtg tga gtcatgtccg acaaccgttt cgacgaggga 927 Thr Ile Thr Ala Glu Val 195 atccgcatcc gccgcgaggt catgggcgac gatttcgtcg acgccgccct gacccgcaac 987 gccggcaccg acggcgagga cctgcagcac cacatcaccg ccaccgtctg gggttcggtg 1047 tggacccgcg agggcctgtc caagcgtgac cgcagcctgc tcaacatcgg catgctcgtc 1107 gccctgcgcg cgaccgagga actgcgcgga catgtccgcg gcggcctgcg taacggcctg 1167 acccgcgagg agatcaccga ggcgatcatc 1197 <210> 116 <211> 198 <212> PRT <213> Corynebacterium humireducens <400> 116 Val Glu Lys Phe Thr Thr His Thr Gly Val Gly Val Pro Leu Thr Arg 1 5 10 15 Ser Asn Val Asp Thr Asp Gln Ile Ile Pro Ala Val Tyr Leu Lys Arg 20 25 30 Val Thr Arg Thr Gly Phe Asp Asp Gly Leu Phe Ala Glu Trp Arg Lys 35 40 45 Asp Asp Ser Phe Val Leu Asn Gln Glu Pro Tyr Arg Asn Gly Ser Val 50 55 60 Leu Val Ala Gly Pro Asp Phe Gly Thr Gly Ser Ser Arg Glu His Ala 65 70 75 80 Val Trp Ala Leu Met Asp Tyr Gly Phe Arg Val Val Leu Ser Ser Arg 85 90 95 Phe Ala Asp Ile Phe Arg Gly Asn Ser Gly Lys Ala Gly Leu Leu Ala 100 105 110 Ala Gln Met Glu Glu Ala Asp Ile Glu Leu Leu Trp Lys Gln Leu Glu 115 120 125 Ala Glu Pro Gly Leu Glu Ile Thr Val Asp Leu Glu Ser Arg Thr Val 130 135 140 Thr Ala Gly Glu Asn Thr Tyr Gln Phe Ser Val Asp Asp Tyr Thr Arg 145 150 155 160 Trp Arg Leu Met Glu Gly Leu Asp Asp Ile Gly Leu Thr Leu Arg Asp 165 170 175 Glu Gln Ala Ile Thr Asp Tyr Glu Ala Lys Arg Pro Val Tyr Lys Pro 180 185 190 Thr Ile Thr Ala Glu Val 195 <210> 117 <211> 1398 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1098) <223> gene panB <400> 117 ccgggcgcaa ggcggacgta ctcgtcattc cgccaacccc ggatgattct ctgacgctct 60 gccccggtgg cacgcgtggt gctgatcacc ggccccccga cctggttcat gtccgcagtc 120 tcccatcagc gcctgcggca cgcacctcca cacgtcggtg gcgttgtgag gcacccgctt 180 gttcgcgagc tccgggagtt ttcccaggtc aggttttcct ggggtgaaca agagggtact 240 tccgcacaca tgccttccgt gggaacaggg gggcatatac tcttgcccat ggtttcccgg 300 atg cgt acc cgc cat ttc gcc cag gcc aag gcc gag ggc cgc aag atc 348 Met Arg Thr Arg His Phe Ala Gln Ala Lys Ala Glu Gly Arg Lys Ile 1 5 10 15 tcc tgc ctg acc gcc tac gac gcg ccc acc gcc gcg atc ttc gat gag 396 Ser Cys Leu Thr Ala Tyr Asp Ala Pro Thr Ala Ala Ile Phe Asp Glu 20 25 30 gcg ggc atc gac ctg ctc ctc gtc ggc gat tcc gcc gcc aac gtc gtc 444 Ala Gly Ile Asp Leu Leu Leu Val Gly Asp Ser Ala Ala Asn Val Val 35 40 45 ctg ggc cag gac tcg acc ctg tcg atc acg gtc gag gag atg atg acc 492 Leu Gly Gln Asp Ser Thr Leu Ser Ile Thr Val Glu Glu Met Met Thr 50 55 60 atg gcg acc gcc gtc gcc cgc ggc acc gaa cgc gcc ttc gtg gtc acc 540 Met Ala Thr Ala Val Ala Arg Gly Thr Glu Arg Ala Phe Val Val Thr 65 70 75 80 gac ctg ccc ttc ggc tcc tac gag gtc tcc ccc gcc cag gcg ctg gag 588 Asp Leu Pro Phe Gly Ser Tyr Glu Val Ser Pro Ala Gln Ala Leu Glu 85 90 95 acg gcc gcc cgg ttc atg aag cag acc ggt gtc gcc gcg gtg aag atc 636 Thr Ala Ala Arg Phe Met Lys Gln Thr Gly Val Ala Ala Val Lys Ile 100 105 110 gag ggc ggc gtc gag atc gcc ccg acg atc cgc acg ctc gtc gac gcc 684 Glu Gly Gly Val Glu Ile Ala Pro Thr Ile Arg Thr Leu Val Asp Ala 115 120 125 ggc atc ccc gtc atg gcc cac atc ggc tac acc ccg cag tcc gag cac 732 Gly Ile Pro Val Met Ala His Ile Gly Tyr Thr Pro Gln Ser Glu His 130 135 140 gcc ctc ggc ggc tac gtc gtc cag ggt cgc ggg gag gcc gcg gag aag 780 Ala Leu Gly Gly Tyr Val Val Gln Gly Arg Gly Glu Ala Ala Glu Lys 145 150 155 160 ctg cgc gcc gac gcc ctc gcc gtc cag gag gcc ggc gcc ttc gcc gtc 828 Leu Arg Ala Asp Ala Leu Ala Val Gln Glu Ala Gly Ala Phe Ala Val 165 170 175 gtc ctc gag atg gtc ccc gcc ggc atc gcc ggg gag atc acc cgc gag 876 Val Leu Glu Met Val Pro Ala Gly Ile Ala Gly Glu Ile Thr Arg Glu 180 185 190 ctc acc atc ccg acg atc ggc atc ggc gcc ggc gcc gac acc gac ggc 924 Leu Thr Ile Pro Thr Ile Gly Ile Gly Ala Gly Ala Asp Thr Asp Gly 195 200 205 cag gtc ctc gtg tgg acc gac gcc ttc ggt ctc acg cgc ggc cgt acg 972 Gln Val Leu Val Trp Thr Asp Ala Phe Gly Leu Thr Arg Gly Arg Thr 210 215 220 ccg cgt ttc gcg cgt cgt tac gcg gag atc ggg gac atg ctc ctc gac 1020 Pro Arg Phe Ala Arg Arg Tyr Ala Glu Ile Gly Asp Met Leu Leu Asp 225 230 235 240 gcc gcc cgt gcc tac cgc acc gac gtc gag tcc ggc tcc ttc ccg gac 1068 Ala Ala Arg Ala Tyr Arg Thr Asp Val Glu Ser Gly Ser Phe Pro Asp 245 250 255 gag tcc gag tcc ttc cgg gac gag cag tga ccgtcgtcgc gcgcactgtc 1118 Glu Ser Glu Ser Phe Arg Asp Glu Gln 260 265 gcgcagctgc gttccgccgt cgccgatctg cagggttccg tcggcctcgt gccgaccatg 1178 ggtgcgctcc acgagggtca cggccgtctg gtgtccctgg ccgccgagga gaactcctcg 1238 gtggtggtga gcatcttcgt caacccgttg cagttcaccg acctcggcga ctgcgacgac 1298 ttccgccact acccgcgtga cctggacaag gacgtcgcct tcctggagaa gctcggcgtc 1358 gacgtcgtgt tcgcgccgac cgacgaggcg atgtacccgg 1398 <210> 118 <211> 265 <212> PRT <213> Corynebacterium humireducens <400> 118 Met Arg Thr Arg His Phe Ala Gln Ala Lys Ala Glu Gly Arg Lys Ile 1 5 10 15 Ser Cys Leu Thr Ala Tyr Asp Ala Pro Thr Ala Ala Ile Phe Asp Glu 20 25 30 Ala Gly Ile Asp Leu Leu Leu Val Gly Asp Ser Ala Ala Asn Val Val 35 40 45 Leu Gly Gln Asp Ser Thr Leu Ser Ile Thr Val Glu Glu Met Met Thr 50 55 60 Met Ala Thr Ala Val Ala Arg Gly Thr Glu Arg Ala Phe Val Val Thr 65 70 75 80 Asp Leu Pro Phe Gly Ser Tyr Glu Val Ser Pro Ala Gln Ala Leu Glu 85 90 95 Thr Ala Ala Arg Phe Met Lys Gln Thr Gly Val Ala Ala Val Lys Ile 100 105 110 Glu Gly Gly Val Glu Ile Ala Pro Thr Ile Arg Thr Leu Val Asp Ala 115 120 125 Gly Ile Pro Val Met Ala His Ile Gly Tyr Thr Pro Gln Ser Glu His 130 135 140 Ala Leu Gly Gly Tyr Val Val Gln Gly Arg Gly Glu Ala Ala Glu Lys 145 150 155 160 Leu Arg Ala Asp Ala Leu Ala Val Gln Glu Ala Gly Ala Phe Ala Val 165 170 175 Val Leu Glu Met Val Pro Ala Gly Ile Ala Gly Glu Ile Thr Arg Glu 180 185 190 Leu Thr Ile Pro Thr Ile Gly Ile Gly Ala Gly Ala Asp Thr Asp Gly 195 200 205 Gln Val Leu Val Trp Thr Asp Ala Phe Gly Leu Thr Arg Gly Arg Thr 210 215 220 Pro Arg Phe Ala Arg Arg Tyr Ala Glu Ile Gly Asp Met Leu Leu Asp 225 230 235 240 Ala Ala Arg Ala Tyr Arg Thr Asp Val Glu Ser Gly Ser Phe Pro Asp 245 250 255 Glu Ser Glu Ser Phe Arg Asp Glu Gln 260 265 <210> 119 <211> 1452 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1152) <223> gene panC <400> 119 cctcgccgtc caggaggccg gcgccttcgc cgtcgtcctc gagatggtcc ccgccggcat 60 cgccggggag atcacccgcg agctcaccat cccgacgatc ggcatcggcg ccggcgccga 120 caccgacggc caggtcctcg tgtggaccga cgccttcggt ctcacgcgcg gccgtacgcc 180 gcgtttcgcg cgtcgttacg cggagatcgg ggacatgctc ctcgacgccg cccgtgccta 240 ccgcaccgac gtcgagtccg gctccttccc ggacgagtcc gagtccttcc gggacgagca 300 gtg acc gtc gtc gcg cgc act gtc gcg cag ctg cgt tcc gcc gtc gcc 348 Val Thr Val Val Ala Arg Thr Val Ala Gln Leu Arg Ser Ala Val Ala 1 5 10 15 gat ctg cag ggt tcc gtc ggc ctc gtg ccg acc atg ggt gcg ctc cac 396 Asp Leu Gln Gly Ser Val Gly Leu Val Pro Thr Met Gly Ala Leu His 20 25 30 gag ggt cac ggc cgt ctg gtg tcc ctg gcc gcc gag gag aac tcc tcg 444 Glu Gly His Gly Arg Leu Val Ser Leu Ala Ala Glu Glu Asn Ser Ser 35 40 45 gtg gtg gtg agc atc ttc gtc aac ccg ttg cag ttc acc gac ctc ggc 492 Val Val Val Ser Ile Phe Val Asn Pro Leu Gln Phe Thr Asp Leu Gly 50 55 60 gac tgc gac gac ttc cgc cac tac ccg cgt gac ctg gac aag gac gtc 540 Asp Cys Asp Asp Phe Arg His Tyr Pro Arg Asp Leu Asp Lys Asp Val 65 70 75 80 gcc ttc ctg gag aag ctc ggc gtc gac gtc gtg ttc gcg ccg acc gac 588 Ala Phe Leu Glu Lys Leu Gly Val Asp Val Val Phe Ala Pro Thr Asp 85 90 95 gag gcg atg tac ccg gac ggc ctg ccc cag gtg tgg gtg cgg gcc ggc 636 Glu Ala Met Tyr Pro Asp Gly Leu Pro Gln Val Trp Val Arg Ala Gly 100 105 110 gag atg ggt gag cgc ctc gag ggc gcc tcc cgt ccc ggc cac ttc gac 684 Glu Met Gly Glu Arg Leu Glu Gly Ala Ser Arg Pro Gly His Phe Asp 115 120 125 ggc gtg acc acc gtg gtg gcc aag ctg ttc cag ctc atc cag ccg gac 732 Gly Val Thr Thr Val Val Ala Lys Leu Phe Gln Leu Ile Gln Pro Asp 130 135 140 cgc gcc tac ttc ggt gag aag gac gcg cag cag ctg gcg atc atc cag 780 Arg Ala Tyr Phe Gly Glu Lys Asp Ala Gln Gln Leu Ala Ile Ile Gln 145 150 155 160 cgc atg gtc atg gac ctg gac ttc ccg ctg cag atc cgc ccg gtg ccg 828 Arg Met Val Met Asp Leu Asp Phe Pro Leu Gln Ile Arg Pro Val Pro 165 170 175 atc atc cgc ggt gcc gac ggc ctg gcc gag tcc tcc cgc aac cag cac 876 Ile Ile Arg Gly Ala Asp Gly Leu Ala Glu Ser Ser Arg Asn Gln His 180 185 190 ctc acc ccc gag gcg cgg gag cag gcg ctc gtc ctg ccg cgc acc ctg 924 Leu Thr Pro Glu Ala Arg Glu Gln Ala Leu Val Leu Pro Arg Thr Leu 195 200 205 gcc gcg ctg cgt gac cgc gcc gcc gcc ggt gac ccc ctc gac gtc gag 972 Ala Ala Leu Arg Asp Arg Ala Ala Ala Gly Asp Pro Leu Asp Val Glu 210 215 220 ggt gcc cgc gag gcg ctg cgc tcc gcc gag ggc gtg gaa ctc gac cac 1020 Gly Ala Arg Glu Ala Leu Arg Ser Ala Glu Gly Val Glu Leu Asp His 225 230 235 240 ctc gag gtc gtc gac gcc gcg acg ctg ctg ccg gtc tcc gag gtc ggc 1068 Leu Glu Val Val Asp Ala Ala Thr Leu Leu Pro Val Ser Glu Val Gly 245 250 255 gcc ggc gcg ctg gcc gtc gcc gcg atc cac gtg ggt ggc acg cgg ctc 1116 Ala Gly Ala Leu Ala Val Ala Ala Ile His Val Gly Gly Thr Arg Leu 260 265 270 atc gac aac gtg cga ctg ctt ccg ccg cgc gct tag cgtaggggcc 1162 Ile Asp Asn Val Arg Leu Leu Pro Pro Arg Ala 275 280 gacgccgacg atcgtcgccg aaccggggcc gagccagtcc ccgtagccca ccaggtgcag 1222 cggcagcccc cggtcgagga cccgccggaa gggacgcagc gccgggcgga agccggtgca 1282 ccagatgagg tgctccgcgt ccacctcgtc gaggctgcgg aacagcggcg tcgcccgcag 1342 gcggccggag tcccgcgcgg ccttcaccgc gggcaggacc acgatgtccc cgagctcgga 1402 gtccgccccc gggtcctcct ccccgcgcag gatcgccagg gcccgcaggc 1452 <210> 120 <211> 283 <212> PRT <213> Corynebacterium humireducens <400> 120 Val Thr Val Val Ala Arg Thr Val Ala Gln Leu Arg Ser Ala Val Ala 1 5 10 15 Asp Leu Gln Gly Ser Val Gly Leu Val Pro Thr Met Gly Ala Leu His 20 25 30 Glu Gly His Gly Arg Leu Val Ser Leu Ala Ala Glu Glu Asn Ser Ser 35 40 45 Val Val Val Ser Ile Phe Val Asn Pro Leu Gln Phe Thr Asp Leu Gly 50 55 60 Asp Cys Asp Asp Phe Arg His Tyr Pro Arg Asp Leu Asp Lys Asp Val 65 70 75 80 Ala Phe Leu Glu Lys Leu Gly Val Asp Val Val Phe Ala Pro Thr Asp 85 90 95 Glu Ala Met Tyr Pro Asp Gly Leu Pro Gln Val Trp Val Arg Ala Gly 100 105 110 Glu Met Gly Glu Arg Leu Glu Gly Ala Ser Arg Pro Gly His Phe Asp 115 120 125 Gly Val Thr Thr Val Val Ala Lys Leu Phe Gln Leu Ile Gln Pro Asp 130 135 140 Arg Ala Tyr Phe Gly Glu Lys Asp Ala Gln Gln Leu Ala Ile Ile Gln 145 150 155 160 Arg Met Val Met Asp Leu Asp Phe Pro Leu Gln Ile Arg Pro Val Pro 165 170 175 Ile Ile Arg Gly Ala Asp Gly Leu Ala Glu Ser Ser Arg Asn Gln His 180 185 190 Leu Thr Pro Glu Ala Arg Glu Gln Ala Leu Val Leu Pro Arg Thr Leu 195 200 205 Ala Ala Leu Arg Asp Arg Ala Ala Ala Gly Asp Pro Leu Asp Val Glu 210 215 220 Gly Ala Arg Glu Ala Leu Arg Ser Ala Glu Gly Val Glu Leu Asp His 225 230 235 240 Leu Glu Val Val Asp Ala Ala Thr Leu Leu Pro Val Ser Glu Val Gly 245 250 255 Ala Gly Ala Leu Ala Val Ala Ala Ile His Val Gly Gly Thr Arg Leu 260 265 270 Ile Asp Asn Val Arg Leu Leu Pro Pro Arg Ala 275 280 <210> 121 <211> 1116 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(816) <223> gene ilvH <400> 121 gcgtcacccg cgaggaggac gtcctgccga cgatccagaa ggcacgcgag atcaacgacc 60 gcccggtcgt catcgacttc atcgtcggtg aggacgccca ggtctggccg atggtcgccg 120 ccggcgcctc caactcggac atcgagtacg cacgtggtct gcgccccttc ttcgagggtg 180 acgagtccgc gggtgagtcc ccggccgaca tccacggtgt catggagaac gcccaggagg 240 agaccgtcga ggaagtcctc gaggagcgcg cgaacgacag cgagaaggag aactaggaca 300 atg gcg aac acc gac atc atc cgc aac acc ctc agc gtc ctg gtc cag 348 Met Ala Asn Thr Asp Ile Ile Arg Asn Thr Leu Ser Val Leu Val Gln 1 5 10 15 gac gtc gac ggc atc atc tcc cgc gtc gca ggc atg ttc acc cgc cgt 396 Asp Val Asp Gly Ile Ile Ser Arg Val Ala Gly Met Phe Thr Arg Arg 20 25 30 ggc tac aac ctg gtg tcc atc gtc tcg gcg cac acc gac acc ccg ggc 444 Gly Tyr Asn Leu Val Ser Ile Val Ser Ala His Thr Asp Thr Pro Gly 35 40 45 atc agc cgc atc acc atc gtg gtc gac gcc aac gag ctg gtc atc gag 492 Ile Ser Arg Ile Thr Ile Val Val Asp Ala Asn Glu Leu Val Ile Glu 50 55 60 cag gtc acc aag cag ctc aac aag atc atc ccg gtg ctc aag gtc gtc 540 Gln Val Thr Lys Gln Leu Asn Lys Ile Ile Pro Val Leu Lys Val Val 65 70 75 80 cgc ctc gag gag gac acc acc atc gcc cgc gcc atc atg ctg gtg aag 588 Arg Leu Glu Glu Asp Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys 85 90 95 gtc aac gcc gac aac acc aac cgc ccg cag gtc gtc gac gcc gcg aac 636 Val Asn Ala Asp Asn Thr Asn Arg Pro Gln Val Val Asp Ala Ala Asn 100 105 110 atc ttc cgc gcc cgc atc gtc gac gtg gcc cag gag tcc gtg gtc atc 684 Ile Phe Arg Ala Arg Ile Val Asp Val Ala Gln Glu Ser Val Val Ile 115 120 125 gag gcc acc ggt acc ccg ggc aag ctg cgc gcc ctg ctg gag gtc ctc 732 Glu Ala Thr Gly Thr Pro Gly Lys Leu Arg Ala Leu Leu Glu Val Leu 130 135 140 gag ccc ttc ggt atc cgt gag ctc atc cag tcc ggt cag atc gcc ctc 780 Glu Pro Phe Gly Ile Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu 145 150 155 160 aac cgc ggt ccg aag acg atg gca ccg tcc aag taa caccccctca 826 Asn Arg Gly Pro Lys Thr Met Ala Pro Ser Lys 165 170 tcccacctgg tgagacaaca cgcatgaaag tcccaccagg tggggtaccc ctgtctcatg 886 ttgtggtaca aacaacaaca gcacttgtat aagacgaaag gcgagttttt ctcccatggc 946 tattgaactg ctttacgacg ccgacgctga cctctccctc atccagggcc gcaaggtcgc 1006 catcatcggc tacggctccc agggccacgc ccacgcccag aacctgcgtg actccggcgt 1066 cgaggtctgc atcggcctgc gcgacggctc caagtccgcg gagaaggcca 1116 <210> 122 <211> 171 <212> PRT <213> Corynebacterium humireducens <400> 122 Met Ala Asn Thr Asp Ile Ile Arg Asn Thr Leu Ser Val Leu Val Gln 1 5 10 15 Asp Val Asp Gly Ile Ile Ser Arg Val Ala Gly Met Phe Thr Arg Arg 20 25 30 Gly Tyr Asn Leu Val Ser Ile Val Ser Ala His Thr Asp Thr Pro Gly 35 40 45 Ile Ser Arg Ile Thr Ile Val Val Asp Ala Asn Glu Leu Val Ile Glu 50 55 60 Gln Val Thr Lys Gln Leu Asn Lys Ile Ile Pro Val Leu Lys Val Val 65 70 75 80 Arg Leu Glu Glu Asp Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys 85 90 95 Val Asn Ala Asp Asn Thr Asn Arg Pro Gln Val Val Asp Ala Ala Asn 100 105 110 Ile Phe Arg Ala Arg Ile Val Asp Val Ala Gln Glu Ser Val Val Ile 115 120 125 Glu Ala Thr Gly Thr Pro Gly Lys Leu Arg Ala Leu Leu Glu Val Leu 130 135 140 Glu Pro Phe Gly Ile Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu 145 150 155 160 Asn Arg Gly Pro Lys Thr Met Ala Pro Ser Lys 165 170 <210> 123 <211> 1944 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1644) <223> gene gdh <400> 123 gaaggtggcg gcggtgccct cacccccgtc ggccatggga gcgagggtga tctcggcgtc 60 ccggatgatc gagcggacac cctcgccgat cagccgtgcg gcctgatcgg cggtggcagt 120 gcccttgaag gagtcggggg cgatgatgat gtggaccatg ggaagcagtc taggggtagt 180 gggagtgaca tattcaactc cggagtgact tgcatgaacc gcgccgcccg tgaaaactat 240 aggccgttag aaaaccccat cccccgggct gcccgcgact gcgggccgga aaggactcgt 300 atg tct att gac gag cag gtc tcc tcc tac tac aac atg ctg ctc aag 348 Met Ser Ile Asp Glu Gln Val Ser Ser Tyr Tyr Asn Met Leu Leu Lys 1 5 10 15 cgg aac gca gga gag ccg gag ttc cac cag gca gtc gca gaa gtt ctc 396 Arg Asn Ala Gly Glu Pro Glu Phe His Gln Ala Val Ala Glu Val Leu 20 25 30 gcc tcc ctc aag atc gtc ctg gag aag gat ccg cac tac gcg gac tac 444 Ala Ser Leu Lys Ile Val Leu Glu Lys Asp Pro His Tyr Ala Asp Tyr 35 40 45 ggc ctc atc cag cgc ctg tgc gag ccg gag cgc cag ctc atc ttc cgt 492 Gly Leu Ile Gln Arg Leu Cys Glu Pro Glu Arg Gln Leu Ile Phe Arg 50 55 60 gtg ccg tgg atc gac gac aac ggc gtg gta cag gtc aac cgt ggt ttc 540 Val Pro Trp Ile Asp Asp Asn Gly Val Val Gln Val Asn Arg Gly Phe 65 70 75 80 cga gta cag ttc aac tcc gcc ctc ggc ccg tac aag ggc ggc cta cgc 588 Arg Val Gln Phe Asn Ser Ala Leu Gly Pro Tyr Lys Gly Gly Leu Arg 85 90 95 ttc cac ccg tcc gtc aac ctg ggc atc atc aag ttc ctc ggc ttc gag 636 Phe His Pro Ser Val Asn Leu Gly Ile Ile Lys Phe Leu Gly Phe Glu 100 105 110 cag atc ttc aag aac tcc ctc acc ggc ctg ccc atc ggc ggc ggc aag 684 Gln Ile Phe Lys Asn Ser Leu Thr Gly Leu Pro Ile Gly Gly Gly Lys 115 120 125 ggc ggc tcc gac ttc gac ccg aag ggc aag tcc gag ctc gag atc atg 732 Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Leu Glu Ile Met 130 135 140 cgt ttc tgc cag tcc ttc atg acc gag ctg cac aag cac atc ggc gag 780 Arg Phe Cys Gln Ser Phe Met Thr Glu Leu His Lys His Ile Gly Glu 145 150 155 160 tac aag gac gtc ccc gcc ggt gac atc ggc gtc ggc ggc cgg gag atc 828 Tyr Lys Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Ile 165 170 175 ggt ttc ctc ttc ggc cag tac cgt cgt ctg gcc ggc cag cac gag tcc 876 Gly Phe Leu Phe Gly Gln Tyr Arg Arg Leu Ala Gly Gln His Glu Ser 180 185 190 ggc gtc ctc acc ggc aag ggc ctc gcc tgg ggt ggc tcc ctc gtg cgc 924 Gly Val Leu Thr Gly Lys Gly Leu Ala Trp Gly Gly Ser Leu Val Arg 195 200 205 acc gag gcc acc ggc tac ggc gcc gtc tac ctc acg cag gag atg atg 972 Thr Glu Ala Thr Gly Tyr Gly Ala Val Tyr Leu Thr Gln Glu Met Met 210 215 220 aag gcc cac ggc gac tcc ctc tcc ggt tcg aag gtc atc gtc tcc ggc 1020 Lys Ala His Gly Asp Ser Leu Ser Gly Ser Lys Val Ile Val Ser Gly 225 230 235 240 tcc ggc aac gtc gcc atc tac gcc atc gag aag gcc cag gag ctc ggc 1068 Ser Gly Asn Val Ala Ile Tyr Ala Ile Glu Lys Ala Gln Glu Leu Gly 245 250 255 gcc acc gtc gtc ggc ttc tcc gac tcc tcc ggc tgg gtc tcc acc ccg 1116 Ala Thr Val Val Gly Phe Ser Asp Ser Ser Gly Trp Val Ser Thr Pro 260 265 270 aac ggc gtc gac gtc gag ctg ctc cac gac gtc aag gag aag cgc cgc 1164 Asn Gly Val Asp Val Glu Leu Leu His Asp Val Lys Glu Lys Arg Arg 275 280 285 gag cgc gtc tcc tgc tac gcc gag gag gcc acc ggc gcg acc ttc cac 1212 Glu Arg Val Ser Cys Tyr Ala Glu Glu Ala Thr Gly Ala Thr Phe His 290 295 300 gcc gag ggc tcc atc tgg gac ctc gcc gcc gac gtc gcc ctc ccg tgc 1260 Ala Glu Gly Ser Ile Trp Asp Leu Ala Ala Asp Val Ala Leu Pro Cys 305 310 315 320 gcc acc cag aac gag ctc aac ggc gac cac gcc cgc acc ctg gcc gac 1308 Ala Thr Gln Asn Glu Leu Asn Gly Asp His Ala Arg Thr Leu Ala Asp 325 330 335 aac ggc atc cgc tac gtc gcc gag ggc gcc aac atg ccc tcc acc ccg 1356 Asn Gly Ile Arg Tyr Val Ala Glu Gly Ala Asn Met Pro Ser Thr Pro 340 345 350 gag gcc atc gag gtc ttc cgt gag cgc ggt atc ttc ttc gcc ccg ggc 1404 Glu Ala Ile Glu Val Phe Arg Glu Arg Gly Ile Phe Phe Ala Pro Gly 355 360 365 aag gcc gcc aac gcc ggt ggc gtg gcc acc tcc gcc ctg gag atg cag 1452 Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Ala Leu Glu Met Gln 370 375 380 cag aac gcc tcc cgt gac tcc tgg tcc ttc gag tac acg gac cag cgc 1500 Gln Asn Ala Ser Arg Asp Ser Trp Ser Phe Glu Tyr Thr Asp Gln Arg 385 390 395 400 ctg cac aag atc atg tcc aag atc ttc cgc acc atg tcc cgc act gcc 1548 Leu His Lys Ile Met Ser Lys Ile Phe Arg Thr Met Ser Arg Thr Ala 405 410 415 gca gag tac ggc cac gag ggc aac tac gtc gtc ggc tcc aac atc gcc 1596 Ala Glu Tyr Gly His Glu Gly Asn Tyr Val Val Gly Ser Asn Ile Ala 420 425 430 ggc ttc aag aag gtc gcc gac gcg atg ctc gcg cag ggc gtc atc taa 1644 Gly Phe Lys Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile 435 440 445 ggctctccac aggattccct gaccccctgg ccctgccgca ccacacattg tgcggcaggg 1704 ccagtaatct ttgagtcatg tatagagtct tcgaagccct ggacgagctc aaccagaccg 1764 tggagcaggc ctacggcgtg cccatgacct ccaactgcat ggtgccgcgc aacgacaccc 1824 tcgcgcttct cgacgatctg cgtaacgccc tgcccgtcga gatcgacgac gcccaggatg 1884 tcctggacca gcgcgacgag atcctccgcg gggccgagga ccgggcccgc gacatgatcg 1944 <210> 124 <211> 447 <212> PRT <213> Corynebacterium humireducens <400> 124 Met Ser Ile Asp Glu Gln Val Ser Ser Tyr Tyr Asn Met Leu Leu Lys 1 5 10 15 Arg Asn Ala Gly Glu Pro Glu Phe His Gln Ala Val Ala Glu Val Leu 20 25 30 Ala Ser Leu Lys Ile Val Leu Glu Lys Asp Pro His Tyr Ala Asp Tyr 35 40 45 Gly Leu Ile Gln Arg Leu Cys Glu Pro Glu Arg Gln Leu Ile Phe Arg 50 55 60 Val Pro Trp Ile Asp Asp Asn Gly Val Val Gln Val Asn Arg Gly Phe 65 70 75 80 Arg Val Gln Phe Asn Ser Ala Leu Gly Pro Tyr Lys Gly Gly Leu Arg 85 90 95 Phe His Pro Ser Val Asn Leu Gly Ile Ile Lys Phe Leu Gly Phe Glu 100 105 110 Gln Ile Phe Lys Asn Ser Leu Thr Gly Leu Pro Ile Gly Gly Gly Lys 115 120 125 Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Leu Glu Ile Met 130 135 140 Arg Phe Cys Gln Ser Phe Met Thr Glu Leu His Lys His Ile Gly Glu 145 150 155 160 Tyr Lys Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Ile 165 170 175 Gly Phe Leu Phe Gly Gln Tyr Arg Arg Leu Ala Gly Gln His Glu Ser 180 185 190 Gly Val Leu Thr Gly Lys Gly Leu Ala Trp Gly Gly Ser Leu Val Arg 195 200 205 Thr Glu Ala Thr Gly Tyr Gly Ala Val Tyr Leu Thr Gln Glu Met Met 210 215 220 Lys Ala His Gly Asp Ser Leu Ser Gly Ser Lys Val Ile Val Ser Gly 225 230 235 240 Ser Gly Asn Val Ala Ile Tyr Ala Ile Glu Lys Ala Gln Glu Leu Gly 245 250 255 Ala Thr Val Val Gly Phe Ser Asp Ser Ser Gly Trp Val Ser Thr Pro 260 265 270 Asn Gly Val Asp Val Glu Leu Leu His Asp Val Lys Glu Lys Arg Arg 275 280 285 Glu Arg Val Ser Cys Tyr Ala Glu Glu Ala Thr Gly Ala Thr Phe His 290 295 300 Ala Glu Gly Ser Ile Trp Asp Leu Ala Ala Asp Val Ala Leu Pro Cys 305 310 315 320 Ala Thr Gln Asn Glu Leu Asn Gly Asp His Ala Arg Thr Leu Ala Asp 325 330 335 Asn Gly Ile Arg Tyr Val Ala Glu Gly Ala Asn Met Pro Ser Thr Pro 340 345 350 Glu Ala Ile Glu Val Phe Arg Glu Arg Gly Ile Phe Phe Ala Pro Gly 355 360 365 Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Ala Leu Glu Met Gln 370 375 380 Gln Asn Ala Ser Arg Asp Ser Trp Ser Phe Glu Tyr Thr Asp Gln Arg 385 390 395 400 Leu His Lys Ile Met Ser Lys Ile Phe Arg Thr Met Ser Arg Thr Ala 405 410 415 Ala Glu Tyr Gly His Glu Gly Asn Tyr Val Val Gly Ser Asn Ile Ala 420 425 430 Gly Phe Lys Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile 435 440 445 <210> 125 <211> 2037 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1737) <223> gene glutaminsynthetase_1 <400> 125 agcgagccgg ggccggtctc ggggaggccg agtttctctc cggggtaccg gccgggtgcg 60 aaggggtcct cgtgttcgcc ggggatctgg gggccgtcga gccagctacg cttggggttc 120 gccataccgt agacattagc cgggggcgtg cgggcagggg aaactcgagt ttcctgcgac 180 tattgatcgg tcaacagata gcagtttaga atgagcgaca ggtaataggg agcggtgccc 240 gcacctgtag actggctgag ataccgcacg acctagagcg tcaacaacca aggagtcatc 300 gtg gcc ttc aac acg atc gaa gaa gtc gtc aag tac atc aag gat gaa 348 Val Ala Phe Asn Thr Ile Glu Glu Val Val Lys Tyr Ile Lys Asp Glu 1 5 10 15 aac gtc gag ttc gtc gac gtc cgc ttc acg gac gtc ccg ggc acc gag 396 Asn Val Glu Phe Val Asp Val Arg Phe Thr Asp Val Pro Gly Thr Glu 20 25 30 cac cac ttc acc atc ccc gcc tcg atg ttc gac gag gag gcg gcg gag 444 His His Phe Thr Ile Pro Ala Ser Met Phe Asp Glu Glu Ala Ala Glu 35 40 45 gaa ggc ctc gcc ttc gac ggc tcc tcc atc cgc ggc ttc acc acg atc 492 Glu Gly Leu Ala Phe Asp Gly Ser Ser Ile Arg Gly Phe Thr Thr Ile 50 55 60 gac gag tcc gac atg aac ctc ctg ccg gac ctc aag acc gcc aag ctc 540 Asp Glu Ser Asp Met Asn Leu Leu Pro Asp Leu Lys Thr Ala Lys Leu 65 70 75 80 gac ccc ttc cgc gag gcc aag acg ctg aac atc aag ttc ttc gtc cac 588 Asp Pro Phe Arg Glu Ala Lys Thr Leu Asn Ile Lys Phe Phe Val His 85 90 95 gac ccc ttc acc cgc gag ccc ttc tcc cgc gac ccc cgc aac gtc gcc 636 Asp Pro Phe Thr Arg Glu Pro Phe Ser Arg Asp Pro Arg Asn Val Ala 100 105 110 cgc aag gcc gag gag tac ctc gcc tcc acc ggc atc gcc gac acc tgc 684 Arg Lys Ala Glu Glu Tyr Leu Ala Ser Thr Gly Ile Ala Asp Thr Cys 115 120 125 ttc ttc ggc gcc gag gcc gag ttc tac ctc ttc gac acc gtc cgg ttc 732 Phe Phe Gly Ala Glu Ala Glu Phe Tyr Leu Phe Asp Thr Val Arg Phe 130 135 140 gag acc tcc acc aac ggc ggc ttc tac gag gtc gac tcc gac gag ggc 780 Glu Thr Ser Thr Asn Gly Gly Phe Tyr Glu Val Asp Ser Asp Glu Gly 145 150 155 160 tgg tgg aac cgc ggc aag gag gag cgt ctc gac ggc cgc cgc aac ctc 828 Trp Trp Asn Arg Gly Lys Glu Glu Arg Leu Asp Gly Arg Arg Asn Leu 165 170 175 ggc tac acc aac cgc gtc aag ggc gga tac ttc ccg gtc gcc ccc tac 876 Gly Tyr Thr Asn Arg Val Lys Gly Gly Tyr Phe Pro Val Ala Pro Tyr 180 185 190 gac cag acc gtg gac atc cgt gac tcc atg gtg cgc cac ctg cag aac 924 Asp Gln Thr Val Asp Ile Arg Asp Ser Met Val Arg His Leu Gln Asn 195 200 205 gcc gac ttc cac atc gag cgc ttc cac aag gag gtg ggc acg ggc ggc 972 Ala Asp Phe His Ile Glu Arg Phe His Lys Glu Val Gly Thr Gly Gly 210 215 220 cag cag gag atc aac tac cgc ttc aac acc ctg ctc cac gcc gcc gat 1020 Gln Gln Glu Ile Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp 225 230 235 240 gat ctg cag acc ttc aag tac atc atc aag aac acc gcc ttc aac gcc 1068 Asp Leu Gln Thr Phe Lys Tyr Ile Ile Lys Asn Thr Ala Phe Asn Ala 245 250 255 ggc aag acg gcc acg ttc atg ccc aag ccc atc gcc ggc gac aac ggt 1116 Gly Lys Thr Ala Thr Phe Met Pro Lys Pro Ile Ala Gly Asp Asn Gly 260 265 270 tcc ggc atg cac gcc cac cag tcg ctg tgg aag gac ggc gag ccg ctc 1164 Ser Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Glu Pro Leu 275 280 285 ttc cac gac gag tcc ggc tac gcc ggc ctg tcc gac atg gcc cgc tac 1212 Phe His Asp Glu Ser Gly Tyr Ala Gly Leu Ser Asp Met Ala Arg Tyr 290 295 300 tac atc ggc ggc atc ctc cac cac gcc ggc gcc gtc ctg gcc ttc acc 1260 Tyr Ile Gly Gly Ile Leu His His Ala Gly Ala Val Leu Ala Phe Thr 305 310 315 320 aac ccg acg ctg aac tcc tac cac cgc ctg gtc aag ggc ttc gag gca 1308 Asn Pro Thr Leu Asn Ser Tyr His Arg Leu Val Lys Gly Phe Glu Ala 325 330 335 ccg atc aac ctg gtg tac tcc cag cgc aac cgc tcc gcc gcg atc cgc 1356 Pro Ile Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg 340 345 350 atc ccg atc acc ggc tcc aac ccg aag gcc aag cgc atc gag ttc cgc 1404 Ile Pro Ile Thr Gly Ser Asn Pro Lys Ala Lys Arg Ile Glu Phe Arg 355 360 365 gcc ccg gac ccg tcc ggc aac ccg tac ctc ggc ttc gcc gcg atg atg 1452 Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Met Met 370 375 380 ctc gcc ggc ctc gac ggc atc aag aac cgc atc gag ccg atg gcc ccg 1500 Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Met Ala Pro 385 390 395 400 gtg gac aag gac ctc tac gag ctc ccg ccg gag gag gcc gcc gcc atc 1548 Val Asp Lys Asp Leu Tyr Glu Leu Pro Pro Glu Glu Ala Ala Ala Ile 405 410 415 ccg cag gcc ccg acc tcc ctc gag gcc tcc ctg gcc gcc ctg cag gag 1596 Pro Gln Ala Pro Thr Ser Leu Glu Ala Ser Leu Ala Ala Leu Gln Glu 420 425 430 gac aac gac ttc ctc acc gag ggt gac gtg ttc acc gag gac ctc atc 1644 Asp Asn Asp Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile 435 440 445 gag gcc tac atc cag ctc aag tac gac aac gag atc acc ccg gcc cgt 1692 Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Thr Pro Ala Arg 450 455 460 ctg cgc ccg acg ccg ctg gag ttc gag ctg tac tac gac tgc tga 1737 Leu Arg Pro Thr Pro Leu Glu Phe Glu Leu Tyr Tyr Asp Cys 465 470 475 ccatgccccc accgcaaccc ccgcgccgga gctttccgac gcgggggttg cgcgcctctc 1797 ccctgtcagc taactgacag taactatact ttccaccgtg aacatatccc tggcaggccc 1857 ccgtcgcctc gcatccctca tcctgtcact gttcctggcg ttgtccctgg cccccgcggc 1917 acacgccgcc accggcagcc cccaggaatt cgtcccctac accgacagcc acggcacaag 1977 ttcgaactac cacttctacc cctccggccg cgagaacgcc ggactcctcg tccacctcga 2037 <210> 126 <211> 478 <212> PRT <213> Corynebacterium humireducens <400> 126 Val Ala Phe Asn Thr Ile Glu Glu Val Val Lys Tyr Ile Lys Asp Glu 1 5 10 15 Asn Val Glu Phe Val Asp Val Arg Phe Thr Asp Val Pro Gly Thr Glu 20 25 30 His His Phe Thr Ile Pro Ala Ser Met Phe Asp Glu Glu Ala Ala Glu 35 40 45 Glu Gly Leu Ala Phe Asp Gly Ser Ser Ile Arg Gly Phe Thr Thr Ile 50 55 60 Asp Glu Ser Asp Met Asn Leu Leu Pro Asp Leu Lys Thr Ala Lys Leu 65 70 75 80 Asp Pro Phe Arg Glu Ala Lys Thr Leu Asn Ile Lys Phe Phe Val His 85 90 95 Asp Pro Phe Thr Arg Glu Pro Phe Ser Arg Asp Pro Arg Asn Val Ala 100 105 110 Arg Lys Ala Glu Glu Tyr Leu Ala Ser Thr Gly Ile Ala Asp Thr Cys 115 120 125 Phe Phe Gly Ala Glu Ala Glu Phe Tyr Leu Phe Asp Thr Val Arg Phe 130 135 140 Glu Thr Ser Thr Asn Gly Gly Phe Tyr Glu Val Asp Ser Asp Glu Gly 145 150 155 160 Trp Trp Asn Arg Gly Lys Glu Glu Arg Leu Asp Gly Arg Arg Asn Leu 165 170 175 Gly Tyr Thr Asn Arg Val Lys Gly Gly Tyr Phe Pro Val Ala Pro Tyr 180 185 190 Asp Gln Thr Val Asp Ile Arg Asp Ser Met Val Arg His Leu Gln Asn 195 200 205 Ala Asp Phe His Ile Glu Arg Phe His Lys Glu Val Gly Thr Gly Gly 210 215 220 Gln Gln Glu Ile Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp 225 230 235 240 Asp Leu Gln Thr Phe Lys Tyr Ile Ile Lys Asn Thr Ala Phe Asn Ala 245 250 255 Gly Lys Thr Ala Thr Phe Met Pro Lys Pro Ile Ala Gly Asp Asn Gly 260 265 270 Ser Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Glu Pro Leu 275 280 285 Phe His Asp Glu Ser Gly Tyr Ala Gly Leu Ser Asp Met Ala Arg Tyr 290 295 300 Tyr Ile Gly Gly Ile Leu His His Ala Gly Ala Val Leu Ala Phe Thr 305 310 315 320 Asn Pro Thr Leu Asn Ser Tyr His Arg Leu Val Lys Gly Phe Glu Ala 325 330 335 Pro Ile Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg 340 345 350 Ile Pro Ile Thr Gly Ser Asn Pro Lys Ala Lys Arg Ile Glu Phe Arg 355 360 365 Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Met Met 370 375 380 Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Met Ala Pro 385 390 395 400 Val Asp Lys Asp Leu Tyr Glu Leu Pro Pro Glu Glu Ala Ala Ala Ile 405 410 415 Pro Gln Ala Pro Thr Ser Leu Glu Ala Ser Leu Ala Ala Leu Gln Glu 420 425 430 Asp Asn Asp Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile 435 440 445 Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Thr Pro Ala Arg 450 455 460 Leu Arg Pro Thr Pro Leu Glu Phe Glu Leu Tyr Tyr Asp Cys 465 470 475 <210> 127 <211> 2037 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1737) <223> gene glutaminsynthetase_2 <400> 127 catccccggc aaccgctggt gggacatgac cgaggagggc atcctgccga aggagcgcct 60 ggtgaacatc ggcgacatcg ccgccggccg tgccctgggc cgcgagaacg acgagcagat 120 cttctgctac tccatcggcg gcatgcccgt ggaggacgtc gcctgggcct gggacgtcta 180 ccacaacgcc ctggagaagg gcatcggcac caggctcaac ctgtgggaca cccccaccct 240 caagtaagcc cctcaagcaa gacacctgaa cacaacaatc taggagccct gacacctgtc 300 gtg tcc ttc cag tcc acc acc gac ctg atc gac ttc atg cgc gat gag 348 Val Ser Phe Gln Ser Thr Thr Asp Leu Ile Asp Phe Met Arg Asp Glu 1 5 10 15 ggg gtg gag tac ctg gac atc cgt ttc acc gat gtc ccc ggc aca gaa 396 Gly Val Glu Tyr Leu Asp Ile Arg Phe Thr Asp Val Pro Gly Thr Glu 20 25 30 cac gcg ctg acg gtt ccc gcc gcg gcg ctg acc gag gag acc gcg gcg 444 His Ala Leu Thr Val Pro Ala Ala Ala Leu Thr Glu Glu Thr Ala Ala 35 40 45 gag ggc ttc gcc ttc gac ggc tcg tcg atc gca ggt ttc acc tcg gtc 492 Glu Gly Phe Ala Phe Asp Gly Ser Ser Ile Ala Gly Phe Thr Ser Val 50 55 60 gac gag tcc gac atg acc ctc atg ccg gac gtc tcg acg gcc tac gtg 540 Asp Glu Ser Asp Met Thr Leu Met Pro Asp Val Ser Thr Ala Tyr Val 65 70 75 80 gac ccc ttc cgt cac cgg aag acg gtg aac atg cag ttc ttc gtc cac 588 Asp Pro Phe Arg His Arg Lys Thr Val Asn Met Gln Phe Phe Val His 85 90 95 gac ccc ttc acc cgc gag gcg ttc tcc cgc gat ccg cgc aac atc gcc 636 Asp Pro Phe Thr Arg Glu Ala Phe Ser Arg Asp Pro Arg Asn Ile Ala 100 105 110 cgc aag gcg gag gag tac atg cgt gcc tcc ggc atc gcc gac gag tgc 684 Arg Lys Ala Glu Glu Tyr Met Arg Ala Ser Gly Ile Ala Asp Glu Cys 115 120 125 tac atc ggc gcc gag gcc gag ttc ttc gtc ttc gac tcg gtc cgc tac 732 Tyr Ile Gly Ala Glu Ala Glu Phe Phe Val Phe Asp Ser Val Arg Tyr 130 135 140 tcc acc gac gtc cac cgt tcc ttc cac cag gtc gac tcc gac gag ggc 780 Ser Thr Asp Val His Arg Ser Phe His Gln Val Asp Ser Asp Glu Gly 145 150 155 160 tgg tgg aac tcc gac gcg gag acc atg atc gac ggt tcc ccg aac ctg 828 Trp Trp Asn Ser Asp Ala Glu Thr Met Ile Asp Gly Ser Pro Asn Leu 165 170 175 ggc aac aag atc cgc gtc aac gac ggc tac ttc ccc gtc gcc ccc tac 876 Gly Asn Lys Ile Arg Val Asn Asp Gly Tyr Phe Pro Val Ala Pro Tyr 180 185 190 gac aag acg atc ccg gtg cgc gac gag atc gcg cac aac ctc tcg cag 924 Asp Lys Thr Ile Pro Val Arg Asp Glu Ile Ala His Asn Leu Ser Gln 195 200 205 gtc ggt ttc gag atc gag cgt ttc cac cac gag gtc tcc acg ggt ggc 972 Val Gly Phe Glu Ile Glu Arg Phe His His Glu Val Ser Thr Gly Gly 210 215 220 atc cag gag gtg aac tac cgc ttc aac acc ctc ctg cac gcg gcg gat 1020 Ile Gln Glu Val Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp 225 230 235 240 gac ctg cag aag ttc aag tac atc gtg aag aac acc gcc gac gcc cac 1068 Asp Leu Gln Lys Phe Lys Tyr Ile Val Lys Asn Thr Ala Asp Ala His 245 250 255 ggc aag gtc gcc acc ttc atg ccg aag ccg ctt ctc gac gac ggc ggc 1116 Gly Lys Val Ala Thr Phe Met Pro Lys Pro Leu Leu Asp Asp Gly Gly 260 265 270 tgc ggc atg cac gcc cac cag tcc ctg tgg aag gac ggc cga ccg ctg 1164 Cys Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Arg Pro Leu 275 280 285 ttc cac gac gag gaa ggt tac gcc ggc ctc tcc gag acc gcc cgc cac 1212 Phe His Asp Glu Glu Gly Tyr Ala Gly Leu Ser Glu Thr Ala Arg His 290 295 300 tac atc ggt ggc ctg ctg gcc cac gcc ccg gcg gtc ctg gcg ttc acc 1260 Tyr Ile Gly Gly Leu Leu Ala His Ala Pro Ala Val Leu Ala Phe Thr 305 310 315 320 aac ccg acg gtc aac tcc tac cgt cgc ctc tac tcc ggt ttc gag gcc 1308 Asn Pro Thr Val Asn Ser Tyr Arg Arg Leu Tyr Ser Gly Phe Glu Ala 325 330 335 ccg gtg aac ctg gtg tac tcc cag cgc aac cgt tcc gcc gcg atc cgt 1356 Pro Val Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg 340 345 350 atc ccg gtg acc ggc ccc tcc gcc gcc gcc aag cgc atc gag ttc cgc 1404 Ile Pro Val Thr Gly Pro Ser Ala Ala Ala Lys Arg Ile Glu Phe Arg 355 360 365 gcc ccg gac ccc tcc ggc aac ccc tac ctc ggt ttc gcg gcc cag ctg 1452 Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Gln Leu 370 375 380 ctg gcc ggt ctg gac ggc atc aag aac cgc atc gag ccg ggc gac ccg 1500 Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Gly Asp Pro 385 390 395 400 gtg gac aag gac ctc tac gag ctg gag ccg gag gag gcc gac aag gtg 1548 Val Asp Lys Asp Leu Tyr Glu Leu Glu Pro Glu Glu Ala Asp Lys Val 405 410 415 ccg cgc gtc ccg cac tcc ctg gag tcc tcg ctg tac gcc ctg gag aac 1596 Pro Arg Val Pro His Ser Leu Glu Ser Ser Leu Tyr Ala Leu Glu Asn 420 425 430 gac cac gag ttc ctc acc gag ggc gac gtg ttc acc gag gac ctc atc 1644 Asp His Glu Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile 435 440 445 gag gcc tac atc cag ctg aag tac gac aac gag atc aac ccg atg cgc 1692 Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Asn Pro Met Arg 450 455 460 caa ggc ccg acg ccg aag gag ttc gag ctc tac ttc aac tgc tag 1737 Gln Gly Pro Thr Pro Lys Glu Phe Glu Leu Tyr Phe Asn Cys 465 470 475 gttcagcagg catgcggcgc agttacgtcc tcggggcgac agagcacacg gacctccggg 1797 gcatccggcg ggtgctcgca cgctaccgct acgacgcgcc gtgggtgctt ctcgacgccc 1857 gccccgtcct cgaggtctcc tggttcgggg agggcgcggt gtcgttctac gccaccactc 1917 ccccgctgcc tcccgacccc gccctggccc gcctcctctt cgacctcggg tcctgcggac 1977 tgctgctcgg ggtgtcgccg gggccgccgg acatcgtcat ctgcggtggt cacagtacgg 2037 <210> 128 <211> 478 <212> PRT <213> Corynebacterium humireducens <400> 128 Val Ser Phe Gln Ser Thr Thr Asp Leu Ile Asp Phe Met Arg Asp Glu 1 5 10 15 Gly Val Glu Tyr Leu Asp Ile Arg Phe Thr Asp Val Pro Gly Thr Glu 20 25 30 His Ala Leu Thr Val Pro Ala Ala Ala Leu Thr Glu Glu Thr Ala Ala 35 40 45 Glu Gly Phe Ala Phe Asp Gly Ser Ser Ile Ala Gly Phe Thr Ser Val 50 55 60 Asp Glu Ser Asp Met Thr Leu Met Pro Asp Val Ser Thr Ala Tyr Val 65 70 75 80 Asp Pro Phe Arg His Arg Lys Thr Val Asn Met Gln Phe Phe Val His 85 90 95 Asp Pro Phe Thr Arg Glu Ala Phe Ser Arg Asp Pro Arg Asn Ile Ala 100 105 110 Arg Lys Ala Glu Glu Tyr Met Arg Ala Ser Gly Ile Ala Asp Glu Cys 115 120 125 Tyr Ile Gly Ala Glu Ala Glu Phe Phe Val Phe Asp Ser Val Arg Tyr 130 135 140 Ser Thr Asp Val His Arg Ser Phe His Gln Val Asp Ser Asp Glu Gly 145 150 155 160 Trp Trp Asn Ser Asp Ala Glu Thr Met Ile Asp Gly Ser Pro Asn Leu 165 170 175 Gly Asn Lys Ile Arg Val Asn Asp Gly Tyr Phe Pro Val Ala Pro Tyr 180 185 190 Asp Lys Thr Ile Pro Val Arg Asp Glu Ile Ala His Asn Leu Ser Gln 195 200 205 Val Gly Phe Glu Ile Glu Arg Phe His His Glu Val Ser Thr Gly Gly 210 215 220 Ile Gln Glu Val Asn Tyr Arg Phe Asn Thr Leu Leu His Ala Ala Asp 225 230 235 240 Asp Leu Gln Lys Phe Lys Tyr Ile Val Lys Asn Thr Ala Asp Ala His 245 250 255 Gly Lys Val Ala Thr Phe Met Pro Lys Pro Leu Leu Asp Asp Gly Gly 260 265 270 Cys Gly Met His Ala His Gln Ser Leu Trp Lys Asp Gly Arg Pro Leu 275 280 285 Phe His Asp Glu Glu Gly Tyr Ala Gly Leu Ser Glu Thr Ala Arg His 290 295 300 Tyr Ile Gly Gly Leu Leu Ala His Ala Pro Ala Val Leu Ala Phe Thr 305 310 315 320 Asn Pro Thr Val Asn Ser Tyr Arg Arg Leu Tyr Ser Gly Phe Glu Ala 325 330 335 Pro Val Asn Leu Val Tyr Ser Gln Arg Asn Arg Ser Ala Ala Ile Arg 340 345 350 Ile Pro Val Thr Gly Pro Ser Ala Ala Ala Lys Arg Ile Glu Phe Arg 355 360 365 Ala Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe Ala Ala Gln Leu 370 375 380 Leu Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Glu Pro Gly Asp Pro 385 390 395 400 Val Asp Lys Asp Leu Tyr Glu Leu Glu Pro Glu Glu Ala Asp Lys Val 405 410 415 Pro Arg Val Pro His Ser Leu Glu Ser Ser Leu Tyr Ala Leu Glu Asn 420 425 430 Asp His Glu Phe Leu Thr Glu Gly Asp Val Phe Thr Glu Asp Leu Ile 435 440 445 Glu Ala Tyr Ile Gln Leu Lys Tyr Asp Asn Glu Ile Asn Pro Met Arg 450 455 460 Gln Gly Pro Thr Pro Lys Glu Phe Glu Leu Tyr Phe Asn Cys 465 470 475 <210> 129 <211> 2241 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1941) <223> gene glutamate synthase <400> 129 ctggggttcc gcgtcgaagg acaccatcaa actggcccgc ctggccaccc agaccggcct 60 gttccccgtg tacgaggcgg agtacggcga gatcacctcg gtgaagaaga tccgccgccc 120 cgccccggtc gaggactacc tccgcctcca gcgccgctac gcgcacctgt tccggaagga 180 cgcggacccg tccgtcatcg agcggctcca ggcgctggcg gaccgcaaca tccgccgcta 240 caacctcatc gacgaagaga ccctcgacga gttcgtcagg ttcgaaggag acgacgcaca 300 gtg agt acc ggt aac cac gtc aac atc ggc atg ccc ttc gcc atc acc 348 Val Ser Thr Gly Asn His Val Asn Ile Gly Met Pro Phe Ala Ile Thr 1 5 10 15 ctg cag acc ggc tcc tcc ctg gcg aac aag acc ggc tcc tgg cgc agc 396 Leu Gln Thr Gly Ser Ser Leu Ala Asn Lys Thr Gly Ser Trp Arg Ser 20 25 30 gag cgg ccc gtg tac gtc gac ctg ctg ccg ccc tgc aac aag gcg tgt 444 Glu Arg Pro Val Tyr Val Asp Leu Leu Pro Pro Cys Asn Lys Ala Cys 35 40 45 ccc gcc ggc ggg aac atc cag ggc tgg ctg tac cac gcc gag gag ggc 492 Pro Ala Gly Gly Asn Ile Gln Gly Trp Leu Tyr His Ala Glu Glu Gly 50 55 60 gac tac gag tcc gcc tgg cgc gag atc atg gtg gac aac ccg ctg ccc 540 Asp Tyr Glu Ser Ala Trp Arg Glu Ile Met Val Asp Asn Pro Leu Pro 65 70 75 80 gcc gtc atg ggg cgt gtc tgc tac cac acc tgc cag acc gcc tgt aac 588 Ala Val Met Gly Arg Val Cys Tyr His Thr Cys Gln Thr Ala Cys Asn 85 90 95 cgc gcg gag gtc gac gag gcc gtc ggc atc aac gcg gtc gag cgc ttc 636 Arg Ala Glu Val Asp Glu Ala Val Gly Ile Asn Ala Val Glu Arg Phe 100 105 110 ctc ggg gac cgt gcc atc gag gag ggg tgg cag gtc gaa ccc gtc aag 684 Leu Gly Asp Arg Ala Ile Glu Glu Gly Trp Gln Val Glu Pro Val Lys 115 120 125 gac ccc tcc ggc cgg agg gtc ctc gtc gtg ggt tcc ggc ccc tcc ggc 732 Asp Pro Ser Gly Arg Arg Val Leu Val Val Gly Ser Gly Pro Ser Gly 130 135 140 ctg tcc gcg gcc tac cac ctc gcc cga ctc ggc cac gag gtg acg gtc 780 Leu Ser Ala Ala Tyr His Leu Ala Arg Leu Gly His Glu Val Thr Val 145 150 155 160 cgc gac cgg ggt gag cgg ccg ggc ggc atg atg cgc tac ggc atc ccc 828 Arg Asp Arg Gly Glu Arg Pro Gly Gly Met Met Arg Tyr Gly Ile Pro 165 170 175 tcc tac cgc ctg ccc cgc cac atc ctc gac gcc gag atc gcg cgc atc 876 Ser Tyr Arg Leu Pro Arg His Ile Leu Asp Ala Glu Ile Ala Arg Ile 180 185 190 gcg gcc ctg ggc gtc acc ttc gag cag ggg gtc acc gtc acc gac gtc 924 Ala Ala Leu Gly Val Thr Phe Glu Gln Gly Val Thr Val Thr Asp Val 195 200 205 gag tcc gaa ctc gag ctt ttc gac gcc gtc ttc acc gcc gtc ggt gcc 972 Glu Ser Glu Leu Glu Leu Phe Asp Ala Val Phe Thr Ala Val Gly Ala 210 215 220 cag ctg ggc cgc aac gtg gag gtg ccc gcc ggg tcg gcg gcg acc atc 1020 Gln Leu Gly Arg Asn Val Glu Val Pro Ala Gly Ser Ala Ala Thr Ile 225 230 235 240 atg aac gcc gtt gac ctg ctg cgt gac acc gag gcc ggg gag gcg ccg 1068 Met Asn Ala Val Asp Leu Leu Arg Asp Thr Glu Ala Gly Glu Ala Pro 245 250 255 ctg ctc ggt cgt cgg gtc gtg gtc tac ggc ggc ggc aac aca gcc gtc 1116 Leu Leu Gly Arg Arg Val Val Val Tyr Gly Gly Gly Asn Thr Ala Val 260 265 270 gac gcc gcc cgc acc gcg cgt cgt ctc ggg gcg acc gag tcg gtc atc 1164 Asp Ala Ala Arg Thr Ala Arg Arg Leu Gly Ala Thr Glu Ser Val Ile 275 280 285 ctc tac cgc cgc acc cgc gac cac atg agc gcc cac gac tcc gag gtc 1212 Leu Tyr Arg Arg Thr Arg Asp His Met Ser Ala His Asp Ser Glu Val 290 295 300 atg gag gcc gag gag gag ggc atc acc atg cgc tgg ctg tcc acc gtc 1260 Met Glu Ala Glu Glu Glu Gly Ile Thr Met Arg Trp Leu Ser Thr Val 305 310 315 320 gac cac gtc gag gag ggc tcc atc agg atc gag aag atg gac atc gac 1308 Asp His Val Glu Glu Gly Ser Ile Arg Ile Glu Lys Met Asp Ile Asp 325 330 335 gcc gac ggc cac ctc acc ccg acc ggt gag tac gag gag ctc gcc gcc 1356 Ala Asp Gly His Leu Thr Pro Thr Gly Glu Tyr Glu Glu Leu Ala Ala 340 345 350 gac tcc ctc gtc atg gcg ctg ggc cag gag tcg gac ctc tcc ctg ctc 1404 Asp Ser Leu Val Met Ala Leu Gly Gln Glu Ser Asp Leu Ser Leu Leu 355 360 365 gag ggc gtg gag ggg gtg gag atc tcc cgc ggc gtc gtc gcc gtc gac 1452 Glu Gly Val Glu Gly Val Glu Ile Ser Arg Gly Val Val Ala Val Asp 370 375 380 gag cag atg atg acc gga cgt gcg ggc gtc ttc gcc ggc ggc gac atg 1500 Glu Gln Met Met Thr Gly Arg Ala Gly Val Phe Ala Gly Gly Asp Met 385 390 395 400 gtg ccg agc gag aag aac gtc acc gtc gcc atc ggc cac ggc aag aag 1548 Val Pro Ser Glu Lys Asn Val Thr Val Ala Ile Gly His Gly Lys Lys 405 410 415 gcg gcc cgg tac atc gac ggt tgg ctc cgt ggc gaa ccc tac gtg ccc 1596 Ala Ala Arg Tyr Ile Asp Gly Trp Leu Arg Gly Glu Pro Tyr Val Pro 420 425 430 gcc gtc aag cct gca gac gcg act ctc gac cgt atg gag acc tgg tac 1644 Ala Val Lys Pro Ala Asp Ala Thr Leu Asp Arg Met Glu Thr Trp Tyr 435 440 445 tac tca gag gcc ccg cac cag gtg cgc gaa cgc ctc gag ggc gcc cgc 1692 Tyr Ser Glu Ala Pro His Gln Val Arg Glu Arg Leu Glu Gly Ala Arg 450 455 460 cgc gcc tcc acc ttt gac gag gtc gtc cgg gga ctc gac gcc gac tcc 1740 Arg Ala Ser Thr Phe Asp Glu Val Val Arg Gly Leu Asp Ala Asp Ser 465 470 475 480 gcc ctg ctc gag gca cgc cgc tgc atg tcg tgc ggc aac tgc ttc ggc 1788 Ala Leu Leu Glu Ala Arg Arg Cys Met Ser Cys Gly Asn Cys Phe Gly 485 490 495 tgc gac aac tgc ttc ggc gtc tgc ccg gac aac gcg gtc acc aaa ttg 1836 Cys Asp Asn Cys Phe Gly Val Cys Pro Asp Asn Ala Val Thr Lys Leu 500 505 510 gcg ccg ggt gag tac gag ttc aag tac gac tac tgc aag ggc tgc ggc 1884 Ala Pro Gly Glu Tyr Glu Phe Lys Tyr Asp Tyr Cys Lys Gly Cys Gly 515 520 525 atc tgc gcg gag gag tgc ccg tgc ggc gcg atc tcc atg att ccc gag 1932 Ile Cys Ala Glu Glu Cys Pro Cys Gly Ala Ile Ser Met Ile Pro Glu 530 535 540 gag aac tga ggagaactga ccgcccgcaa caagttagag tatgggcacc 1981 Glu Asn 545 aacgtcgtcc cctgaccaga aggatcccca tgcgccgcac cctcatcgcc ctcggcaccg 2041 ccaccgccct caccctgggg gcggtcccgg ccgcccacgc ccagcccgcg aacgccgtca 2101 ccgcccagat cgaggagctg ccggccgaca tgcgggcagg ctcctccggc gcggagaagc 2161 tctcctccgc ctccaccgag gccgagaagc aggagggtgc cctgctgctg ggacgcgact 2221 ggctgctcgg tttcgccgcg 2241 <210> 130 <211> 546 <212> PRT <213> Corynebacterium humireducens <400> 130 Val Ser Thr Gly Asn His Val Asn Ile Gly Met Pro Phe Ala Ile Thr 1 5 10 15 Leu Gln Thr Gly Ser Ser Leu Ala Asn Lys Thr Gly Ser Trp Arg Ser 20 25 30 Glu Arg Pro Val Tyr Val Asp Leu Leu Pro Pro Cys Asn Lys Ala Cys 35 40 45 Pro Ala Gly Gly Asn Ile Gln Gly Trp Leu Tyr His Ala Glu Glu Gly 50 55 60 Asp Tyr Glu Ser Ala Trp Arg Glu Ile Met Val Asp Asn Pro Leu Pro 65 70 75 80 Ala Val Met Gly Arg Val Cys Tyr His Thr Cys Gln Thr Ala Cys Asn 85 90 95 Arg Ala Glu Val Asp Glu Ala Val Gly Ile Asn Ala Val Glu Arg Phe 100 105 110 Leu Gly Asp Arg Ala Ile Glu Glu Gly Trp Gln Val Glu Pro Val Lys 115 120 125 Asp Pro Ser Gly Arg Arg Val Leu Val Val Gly Ser Gly Pro Ser Gly 130 135 140 Leu Ser Ala Ala Tyr His Leu Ala Arg Leu Gly His Glu Val Thr Val 145 150 155 160 Arg Asp Arg Gly Glu Arg Pro Gly Gly Met Met Arg Tyr Gly Ile Pro 165 170 175 Ser Tyr Arg Leu Pro Arg His Ile Leu Asp Ala Glu Ile Ala Arg Ile 180 185 190 Ala Ala Leu Gly Val Thr Phe Glu Gln Gly Val Thr Val Thr Asp Val 195 200 205 Glu Ser Glu Leu Glu Leu Phe Asp Ala Val Phe Thr Ala Val Gly Ala 210 215 220 Gln Leu Gly Arg Asn Val Glu Val Pro Ala Gly Ser Ala Ala Thr Ile 225 230 235 240 Met Asn Ala Val Asp Leu Leu Arg Asp Thr Glu Ala Gly Glu Ala Pro 245 250 255 Leu Leu Gly Arg Arg Val Val Val Tyr Gly Gly Gly Asn Thr Ala Val 260 265 270 Asp Ala Ala Arg Thr Ala Arg Arg Leu Gly Ala Thr Glu Ser Val Ile 275 280 285 Leu Tyr Arg Arg Thr Arg Asp His Met Ser Ala His Asp Ser Glu Val 290 295 300 Met Glu Ala Glu Glu Glu Gly Ile Thr Met Arg Trp Leu Ser Thr Val 305 310 315 320 Asp His Val Glu Glu Gly Ser Ile Arg Ile Glu Lys Met Asp Ile Asp 325 330 335 Ala Asp Gly His Leu Thr Pro Thr Gly Glu Tyr Glu Glu Leu Ala Ala 340 345 350 Asp Ser Leu Val Met Ala Leu Gly Gln Glu Ser Asp Leu Ser Leu Leu 355 360 365 Glu Gly Val Glu Gly Val Glu Ile Ser Arg Gly Val Val Ala Val Asp 370 375 380 Glu Gln Met Met Thr Gly Arg Ala Gly Val Phe Ala Gly Gly Asp Met 385 390 395 400 Val Pro Ser Glu Lys Asn Val Thr Val Ala Ile Gly His Gly Lys Lys 405 410 415 Ala Ala Arg Tyr Ile Asp Gly Trp Leu Arg Gly Glu Pro Tyr Val Pro 420 425 430 Ala Val Lys Pro Ala Asp Ala Thr Leu Asp Arg Met Glu Thr Trp Tyr 435 440 445 Tyr Ser Glu Ala Pro His Gln Val Arg Glu Arg Leu Glu Gly Ala Arg 450 455 460 Arg Ala Ser Thr Phe Asp Glu Val Val Arg Gly Leu Asp Ala Asp Ser 465 470 475 480 Ala Leu Leu Glu Ala Arg Arg Cys Met Ser Cys Gly Asn Cys Phe Gly 485 490 495 Cys Asp Asn Cys Phe Gly Val Cys Pro Asp Asn Ala Val Thr Lys Leu 500 505 510 Ala Pro Gly Glu Tyr Glu Phe Lys Tyr Asp Tyr Cys Lys Gly Cys Gly 515 520 525 Ile Cys Ala Glu Glu Cys Pro Cys Gly Ala Ile Ser Met Ile Pro Glu 530 535 540 Glu Asn 545 <210> 131 <211> 2817 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2517) <223> gene isocitrate dehydrogenase <400> 131 cgccgacgta gtgccagccc gcgtcgaggg cgggctgcaa cgccttgacg gactcctcga 60 cggtcgcgcg cacctcctgc aggagcacca cgtcagccgg ggactgctcc agccaggcca 120 gcatgcccgg attgtcctcg ttgcgctgct tgacggcggc gcggattcca ttgacgttga 180 cggaggtgat ggtcagactc ataggaacca ccatatcgcc gttggtggca gggcccctgc 240 cccgccgggt atccttagcc gttgacactg ccgagattga agaaagcgtt agggagttac 300 atg gca aag atc atc tgg acc cgc acc gac gag gcc ccg ctg ctc gcg 348 Met Ala Lys Ile Ile Trp Thr Arg Thr Asp Glu Ala Pro Leu Leu Ala 1 5 10 15 acc tat tcg ttc aag cct gtt gtt gag gcc ttc gcc gcc acc gcg ggt 396 Thr Tyr Ser Phe Lys Pro Val Val Glu Ala Phe Ala Ala Thr Ala Gly 20 25 30 atc gat gtc gag acc cgc gac atc tcc ctg gcc ggc cgc atc ctg gcc 444 Ile Asp Val Glu Thr Arg Asp Ile Ser Leu Ala Gly Arg Ile Leu Ala 35 40 45 cag ttc ccg gac cgc ctc acc gag gag cag cga gtc gct gac gcc ctc 492 Gln Phe Pro Asp Arg Leu Thr Glu Glu Gln Arg Val Ala Asp Ala Leu 50 55 60 acc gag ctc ggc gac ctg gcc aag acc ccg gaa gcc aac atc atc aag 540 Thr Glu Leu Gly Asp Leu Ala Lys Thr Pro Glu Ala Asn Ile Ile Lys 65 70 75 80 ctc ccg aac atc tcg gcg tcc gtc ccg cag ctc aag gca gcc atc aag 588 Leu Pro Asn Ile Ser Ala Ser Val Pro Gln Leu Lys Ala Ala Ile Lys 85 90 95 gag ctg cag gac cag gga tac gac ctc ccc gac tac gtc gac tcc ccg 636 Glu Leu Gln Asp Gln Gly Tyr Asp Leu Pro Asp Tyr Val Asp Ser Pro 100 105 110 gag acc gac gag gag aag gac gcc cgc aag cgc tac gac gta gta aag 684 Glu Thr Asp Glu Glu Lys Asp Ala Arg Lys Arg Tyr Asp Val Val Lys 115 120 125 ggt tcc gct gtg aac ccg gta ctc cgt gag ggc aac tcc gac cgt cgt 732 Gly Ser Ala Val Asn Pro Val Leu Arg Glu Gly Asn Ser Asp Arg Arg 130 135 140 gcc ccg gag gcc gtg aag aac ttc gtc cgc aag aac ccg cac cgc atg 780 Ala Pro Glu Ala Val Lys Asn Phe Val Arg Lys Asn Pro His Arg Met 145 150 155 160 ggt gag tgg tcc gcc gac tcc aag acc aac gtc gcc acc atg gac tcc 828 Gly Glu Trp Ser Ala Asp Ser Lys Thr Asn Val Ala Thr Met Asp Ser 165 170 175 gac gac ttc cgt cac aac gag cag tcc gtc atc ctc ccg gcc gag gac 876 Asp Asp Phe Arg His Asn Glu Gln Ser Val Ile Leu Pro Ala Glu Asp 180 185 190 acc ctc tcc ttc gtc cac gtc gcc acc gac ggc acc gag acc aag ctc 924 Thr Leu Ser Phe Val His Val Ala Thr Asp Gly Thr Glu Thr Lys Leu 195 200 205 cgc gac gac ctc aag gtc ctc gag ggc gag gtc gtc gac ggc acc gtc 972 Arg Asp Asp Leu Lys Val Leu Glu Gly Glu Val Val Asp Gly Thr Val 210 215 220 atg cgc gcc gcc gcc ctg cag gag ttc ctg gcc gag cag gtc aag cgc 1020 Met Arg Ala Ala Ala Leu Gln Glu Phe Leu Ala Glu Gln Val Lys Arg 225 230 235 240 gcc cag gac gag ggc atc ctg ttc tcc gtc cac ctc aag gcc acc atg 1068 Ala Gln Asp Glu Gly Ile Leu Phe Ser Val His Leu Lys Ala Thr Met 245 250 255 atg aag gtc tcc gac ccg atc atc ttc ggc tac gtc gtc cgc gcc ttc 1116 Met Lys Val Ser Asp Pro Ile Ile Phe Gly Tyr Val Val Arg Ala Phe 260 265 270 ttc gcc gac gtc tac gag aag tac ggc gag gag ctg ctg gcc gcc ggt 1164 Phe Ala Asp Val Tyr Glu Lys Tyr Gly Glu Glu Leu Leu Ala Ala Gly 275 280 285 ctc gac ggc gag aac ggc ctc ggc gcc atc ttc tcc gga ctc gag gaa 1212 Leu Asp Gly Glu Asn Gly Leu Gly Ala Ile Phe Ser Gly Leu Glu Glu 290 295 300 ctg gag aac ggc gag gag atc cgc cgt gcc ttc gac gag gcc ctc gag 1260 Leu Glu Asn Gly Glu Glu Ile Arg Arg Ala Phe Asp Glu Ala Leu Glu 305 310 315 320 aac ggc cct gac ctg gcg atg gtg aac tcc gac aag ggc atc acc aac 1308 Asn Gly Pro Asp Leu Ala Met Val Asn Ser Asp Lys Gly Ile Thr Asn 325 330 335 ctg cac gtc ccg tcc gac gtc atc gtc gac gcc tcc atg ccc gcc atg 1356 Leu His Val Pro Ser Asp Val Ile Val Asp Ala Ser Met Pro Ala Met 340 345 350 atc cgc acc tcc ggc cag atg tgg aac aag gac gac aag acc cag gac 1404 Ile Arg Thr Ser Gly Gln Met Trp Asn Lys Asp Asp Lys Thr Gln Asp 355 360 365 acc ctg gcc gtc atc ccg gac tcc tcc tac gcc ggc gtc ttc cag acc 1452 Thr Leu Ala Val Ile Pro Asp Ser Ser Tyr Ala Gly Val Phe Gln Thr 370 375 380 gtc atc gac gac tgc aag gcc aac ggc gcc ttc gac ccg acc acc atg 1500 Val Ile Asp Asp Cys Lys Ala Asn Gly Ala Phe Asp Pro Thr Thr Met 385 390 395 400 ggc acc gtc ccg aac gtc ggc ctc atg gcc cag aag gcc gag gag tac 1548 Gly Thr Val Pro Asn Val Gly Leu Met Ala Gln Lys Ala Glu Glu Tyr 405 410 415 ggc tcc cac gac aag acc ttc cgc atc gcc gcg gac ggc aag gtc gag 1596 Gly Ser His Asp Lys Thr Phe Arg Ile Ala Ala Asp Gly Lys Val Glu 420 425 430 gtc cgc aac tcc gcc ggt gac gtc ctg ctc gag cac gag gtc tcc gag 1644 Val Arg Asn Ser Ala Gly Asp Val Leu Leu Glu His Glu Val Ser Glu 435 440 445 ggc gac atc tgg cgc gcc tgc cag acc aag gac gcc ccg atc cag gac 1692 Gly Asp Ile Trp Arg Ala Cys Gln Thr Lys Asp Ala Pro Ile Gln Asp 450 455 460 tgg gtc aag ctg gcc gtc gac cgc gcc cgc atc tcc ggc atg ccg gcc 1740 Trp Val Lys Leu Ala Val Asp Arg Ala Arg Ile Ser Gly Met Pro Ala 465 470 475 480 gtc ttc tgg ctg gac ccg gag cgc gcc cac gac cgc aac ctc acc acc 1788 Val Phe Trp Leu Asp Pro Glu Arg Ala His Asp Arg Asn Leu Thr Thr 485 490 495 ctg gtg gag aag tac ctg gcc gac cac gac acc gag ggc ctg gac atc 1836 Leu Val Glu Lys Tyr Leu Ala Asp His Asp Thr Glu Gly Leu Asp Ile 500 505 510 cgc atc ctc tcc ccg gtc gag gcg acc cag ctg tcc gtc gac cgc atc 1884 Arg Ile Leu Ser Pro Val Glu Ala Thr Gln Leu Ser Val Asp Arg Ile 515 520 525 cgc cgc ggc gag gac acc atc tcc gtg acc ggt aac gtc ctg cgt gac 1932 Arg Arg Gly Glu Asp Thr Ile Ser Val Thr Gly Asn Val Leu Arg Asp 530 535 540 tac aac acc gac ctc ttc ccg atc ctc gag ctg ggc acc tcc gcc aag 1980 Tyr Asn Thr Asp Leu Phe Pro Ile Leu Glu Leu Gly Thr Ser Ala Lys 545 550 555 560 atg ctc tcc gtc gtg ccg ctg atg gcc ggt ggc ggc ctc ttc gag acc 2028 Met Leu Ser Val Val Pro Leu Met Ala Gly Gly Gly Leu Phe Glu Thr 565 570 575 ggt gcc ggt ggc tcc gcc ccg aag cac gtc cag cag gtc gtc gag gag 2076 Gly Ala Gly Gly Ser Ala Pro Lys His Val Gln Gln Val Val Glu Glu 580 585 590 aac cac ctg cgc tgg gac tcc ctc ggt gag ttc ctc gcc ctg gcc gag 2124 Asn His Leu Arg Trp Asp Ser Leu Gly Glu Phe Leu Ala Leu Ala Glu 595 600 605 tcc ctg cgc cac gtc gcc cgc acc cag gac aac gcg aag gcc gcc gtc 2172 Ser Leu Arg His Val Ala Arg Thr Gln Asp Asn Ala Lys Ala Ala Val 610 615 620 ctg gcc gac gcc ctg gac aag gcc acc gag aag gtc ctg tcc gag ggc 2220 Leu Ala Asp Ala Leu Asp Lys Ala Thr Glu Lys Val Leu Ser Glu Gly 625 630 635 640 aag tcc ccg tcc cgc aag gtc ggc gag atc gac aac cgt ggc tcc cac 2268 Lys Ser Pro Ser Arg Lys Val Gly Glu Ile Asp Asn Arg Gly Ser His 645 650 655 ttc tac ctc gcg tcc tac tgg gcc gag gcc ctg gcc gca cag tcc gac 2316 Phe Tyr Leu Ala Ser Tyr Trp Ala Glu Ala Leu Ala Ala Gln Ser Asp 660 665 670 gac gcc gag ctg gcc gcg acc ttc cag ccg gtc gcc gag gcc ctg gcc 2364 Asp Ala Glu Leu Ala Ala Thr Phe Gln Pro Val Ala Glu Ala Leu Ala 675 680 685 gcc gac gcc gcg acc atc gac cag gag ctc atc gac aac cag ggt tcc 2412 Ala Asp Ala Ala Thr Ile Asp Gln Glu Leu Ile Asp Asn Gln Gly Ser 690 695 700 gcc gtg gac ctc ggc ggc tac tac gcc ccg tcc gat gag aag acc tcc 2460 Ala Val Asp Leu Gly Gly Tyr Tyr Ala Pro Ser Asp Glu Lys Thr Ser 705 710 715 720 gac gtg atg cgt ccg tcc gcg aag ttc aac gag atc atc gac ggt ctg 2508 Asp Val Met Arg Pro Ser Ala Lys Phe Asn Glu Ile Ile Asp Gly Leu 725 730 735 aag aag taa cccccgcttt ccgacgccgc cctgccccgc caccggggca 2557 Lys Lys gggcggcgtc gcctttgggc agggccccgg acgaaccact tggtctaccg tccctttatt 2617 tttattcatg cgccctgaac tgcggagaca gggtttggac agacagctcg gtttacgtcg 2677 aaaagcggtg cgccgtactc ttctcccatg acccgatacg acaactccgc agcagaccag 2737 tgggacttcg agacccgctc catccacgcc ggccagaacc cggaccccgc gaccggttcc 2797 cgtaaccagc cgatccacct 2817 <210> 132 <211> 738 <212> PRT <213> Corynebacterium humireducens <400> 132 Met Ala Lys Ile Ile Trp Thr Arg Thr Asp Glu Ala Pro Leu Leu Ala 1 5 10 15 Thr Tyr Ser Phe Lys Pro Val Val Glu Ala Phe Ala Ala Thr Ala Gly 20 25 30 Ile Asp Val Glu Thr Arg Asp Ile Ser Leu Ala Gly Arg Ile Leu Ala 35 40 45 Gln Phe Pro Asp Arg Leu Thr Glu Glu Gln Arg Val Ala Asp Ala Leu 50 55 60 Thr Glu Leu Gly Asp Leu Ala Lys Thr Pro Glu Ala Asn Ile Ile Lys 65 70 75 80 Leu Pro Asn Ile Ser Ala Ser Val Pro Gln Leu Lys Ala Ala Ile Lys 85 90 95 Glu Leu Gln Asp Gln Gly Tyr Asp Leu Pro Asp Tyr Val Asp Ser Pro 100 105 110 Glu Thr Asp Glu Glu Lys Asp Ala Arg Lys Arg Tyr Asp Val Val Lys 115 120 125 Gly Ser Ala Val Asn Pro Val Leu Arg Glu Gly Asn Ser Asp Arg Arg 130 135 140 Ala Pro Glu Ala Val Lys Asn Phe Val Arg Lys Asn Pro His Arg Met 145 150 155 160 Gly Glu Trp Ser Ala Asp Ser Lys Thr Asn Val Ala Thr Met Asp Ser 165 170 175 Asp Asp Phe Arg His Asn Glu Gln Ser Val Ile Leu Pro Ala Glu Asp 180 185 190 Thr Leu Ser Phe Val His Val Ala Thr Asp Gly Thr Glu Thr Lys Leu 195 200 205 Arg Asp Asp Leu Lys Val Leu Glu Gly Glu Val Val Asp Gly Thr Val 210 215 220 Met Arg Ala Ala Ala Leu Gln Glu Phe Leu Ala Glu Gln Val Lys Arg 225 230 235 240 Ala Gln Asp Glu Gly Ile Leu Phe Ser Val His Leu Lys Ala Thr Met 245 250 255 Met Lys Val Ser Asp Pro Ile Ile Phe Gly Tyr Val Val Arg Ala Phe 260 265 270 Phe Ala Asp Val Tyr Glu Lys Tyr Gly Glu Glu Leu Leu Ala Ala Gly 275 280 285 Leu Asp Gly Glu Asn Gly Leu Gly Ala Ile Phe Ser Gly Leu Glu Glu 290 295 300 Leu Glu Asn Gly Glu Glu Ile Arg Arg Ala Phe Asp Glu Ala Leu Glu 305 310 315 320 Asn Gly Pro Asp Leu Ala Met Val Asn Ser Asp Lys Gly Ile Thr Asn 325 330 335 Leu His Val Pro Ser Asp Val Ile Val Asp Ala Ser Met Pro Ala Met 340 345 350 Ile Arg Thr Ser Gly Gln Met Trp Asn Lys Asp Asp Lys Thr Gln Asp 355 360 365 Thr Leu Ala Val Ile Pro Asp Ser Ser Tyr Ala Gly Val Phe Gln Thr 370 375 380 Val Ile Asp Asp Cys Lys Ala Asn Gly Ala Phe Asp Pro Thr Thr Met 385 390 395 400 Gly Thr Val Pro Asn Val Gly Leu Met Ala Gln Lys Ala Glu Glu Tyr 405 410 415 Gly Ser His Asp Lys Thr Phe Arg Ile Ala Ala Asp Gly Lys Val Glu 420 425 430 Val Arg Asn Ser Ala Gly Asp Val Leu Leu Glu His Glu Val Ser Glu 435 440 445 Gly Asp Ile Trp Arg Ala Cys Gln Thr Lys Asp Ala Pro Ile Gln Asp 450 455 460 Trp Val Lys Leu Ala Val Asp Arg Ala Arg Ile Ser Gly Met Pro Ala 465 470 475 480 Val Phe Trp Leu Asp Pro Glu Arg Ala His Asp Arg Asn Leu Thr Thr 485 490 495 Leu Val Glu Lys Tyr Leu Ala Asp His Asp Thr Glu Gly Leu Asp Ile 500 505 510 Arg Ile Leu Ser Pro Val Glu Ala Thr Gln Leu Ser Val Asp Arg Ile 515 520 525 Arg Arg Gly Glu Asp Thr Ile Ser Val Thr Gly Asn Val Leu Arg Asp 530 535 540 Tyr Asn Thr Asp Leu Phe Pro Ile Leu Glu Leu Gly Thr Ser Ala Lys 545 550 555 560 Met Leu Ser Val Val Pro Leu Met Ala Gly Gly Gly Leu Phe Glu Thr 565 570 575 Gly Ala Gly Gly Ser Ala Pro Lys His Val Gln Gln Val Val Glu Glu 580 585 590 Asn His Leu Arg Trp Asp Ser Leu Gly Glu Phe Leu Ala Leu Ala Glu 595 600 605 Ser Leu Arg His Val Ala Arg Thr Gln Asp Asn Ala Lys Ala Ala Val 610 615 620 Leu Ala Asp Ala Leu Asp Lys Ala Thr Glu Lys Val Leu Ser Glu Gly 625 630 635 640 Lys Ser Pro Ser Arg Lys Val Gly Glu Ile Asp Asn Arg Gly Ser His 645 650 655 Phe Tyr Leu Ala Ser Tyr Trp Ala Glu Ala Leu Ala Ala Gln Ser Asp 660 665 670 Asp Ala Glu Leu Ala Ala Thr Phe Gln Pro Val Ala Glu Ala Leu Ala 675 680 685 Ala Asp Ala Ala Thr Ile Asp Gln Glu Leu Ile Asp Asn Gln Gly Ser 690 695 700 Ala Val Asp Leu Gly Gly Tyr Tyr Ala Pro Ser Asp Glu Lys Thr Ser 705 710 715 720 Asp Val Met Arg Pro Ser Ala Lys Phe Asn Glu Ile Ile Asp Gly Leu 725 730 735 Lys Lys <210> 133 <211> 3378 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(3078) <223> gene aconitathydrase <400> 133 aggtcatcgg tgtcgatgcc ggcgggaatc atcgtggtgc ggcttctttc gcatggggcg 60 gcgtgcggac accgctattt ctatcctccg cgggtgtggc gggggtggcg acgcgccgga 120 cacacagcga aaagccacaa acaagacgct cgtactgtta gaattgcagc ggtcgttggt 180 ccgaatccct acacttgggg acgtaccacc gcgttctgcc ggtgcacgcc taccttgacc 240 ggggtagacg ggtgcgggta acgaacattc cagttcaatg aagaggaaat ggagctccct 300 gtg acc gaa agc aag aac tcc ttc aac gcc aag cgc acc ctc gag gtc 348 Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Arg Thr Leu Glu Val 1 5 10 15 ggc gag aag tcc tac gac tac ttc gcc ctc gac gcc gtg ccg ggc atg 396 Gly Glu Lys Ser Tyr Asp Tyr Phe Ala Leu Asp Ala Val Pro Gly Met 20 25 30 gag aag ctg ccg tac tcc ctg aag gtt ctc ggc gag aac ctt ctg cgt 444 Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg 35 40 45 acc gag gac ggc gca aac atc acc acc gag cac atc gag gca atc gcc 492 Thr Glu Asp Gly Ala Asn Ile Thr Thr Glu His Ile Glu Ala Ile Ala 50 55 60 aac tgg gat ccg aag gct gag ccc tcc atc gag atc cag ttc acc ccg 540 Asn Trp Asp Pro Lys Ala Glu Pro Ser Ile Glu Ile Gln Phe Thr Pro 65 70 75 80 gca cgt gtc ctc atg cag gac ttc acc ggt gtc gcc tgt gca gtc gac 588 Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Ala Cys Ala Val Asp 85 90 95 ctg gcc acc atg cgc gag gcc gtg aag acc ctg ggc ggc gac ccg gac 636 Leu Ala Thr Met Arg Glu Ala Val Lys Thr Leu Gly Gly Asp Pro Asp 100 105 110 gac gtc aac ccg ctg aac ccg gcc gag atg gtc atc gac cac tcc gtc 684 Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val 115 120 125 atc gtc gag gca ttc ggt acc tcc gcc gct ctg gag aag aac gtc gag 732 Ile Val Glu Ala Phe Gly Thr Ser Ala Ala Leu Glu Lys Asn Val Glu 130 135 140 atc gag tac cag cgc aac gag gag cgc tac cag ttc ctc cgc tgg ggt 780 Ile Glu Tyr Gln Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly 145 150 155 160 gcc gag aac ttc tcc aac ttc cgc gtc gtt ccc ccg gga acc ggc atc 828 Ala Glu Asn Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile 165 170 175 gtc cac cag gtc aac atc gag tac ctg tcc cgc gtt gtc ttc gac aac 876 Val His Gln Val Asn Ile Glu Tyr Leu Ser Arg Val Val Phe Asp Asn 180 185 190 aac ggc ctc gcc tac ccg gac acc tgc atc ggc acc gac tcc cac acc 924 Asn Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr 195 200 205 acc atg gag aac ggc ctg gga atc ctg ggc tgg ggc gtc ggc ggc atc 972 Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile 210 215 220 gag gcc gag gct gcc atg ctg ggc cag ccg gtc tcc atg ctc atc ccg 1020 Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro 225 230 235 240 cgc gtc gtc ggc ttc aag ctg acc ggt gag atc ccg gcc ggc gtc acc 1068 Arg Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro Ala Gly Val Thr 245 250 255 gcc acc gac gtg gtc ctc acc atc acc gag atg ctg cgt aac cac ggc 1116 Ala Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu Arg Asn His Gly 260 265 270 gtc gtc cag aag ttc gtc gag ttc tac ggc aac ggc gtc aag tcc gtc 1164 Val Val Gln Lys Phe Val Glu Phe Tyr Gly Asn Gly Val Lys Ser Val 275 280 285 ccg ctg gcg aac cgc gcc acc atc ggc aac atg tcc ccg gag ttc ggc 1212 Pro Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser Pro Glu Phe Gly 290 295 300 tcc acc tgt gcc atg ttc ccg atc gac gag gag acc atc aag tac ctc 1260 Ser Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr Ile Lys Tyr Leu 305 310 315 320 gag ctc acc ggc cgc tcc gag gag gac atc gcc cgc gtc gag gca tac 1308 Glu Leu Thr Gly Arg Ser Glu Glu Asp Ile Ala Arg Val Glu Ala Tyr 325 330 335 gcc aag gct cag ggc atg tgg ctc tac gag gac gcc ccg gag gcc gag 1356 Ala Lys Ala Gln Gly Met Trp Leu Tyr Glu Asp Ala Pro Glu Ala Glu 340 345 350 tac tcc gag tac ctc gag ctg gac ctc tcc acc gtc gtc ccg tcc atc 1404 Tyr Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val Val Pro Ser Ile 355 360 365 gcc ggc ccg aag cgc ccg cag gac cgc atc ctc ctc tcc gag gcc aag 1452 Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu Ser Glu Ala Lys 370 375 380 gca cag ttc cgc aag gac ctg ccg acc tac gcc tcc gac gag gtc gct 1500 Ala Gln Phe Arg Lys Asp Leu Pro Thr Tyr Ala Ser Asp Glu Val Ala 385 390 395 400 gag ccg gtc cgc gcc acc aag gcc gtc ggc gac gac tac aac cag tcc 1548 Glu Pro Val Arg Ala Thr Lys Ala Val Gly Asp Asp Tyr Asn Gln Ser 405 410 415 ttc ccg ggc aac ggc gag tcc gct gcc gac tcc gcc gag ggc cgc gcc 1596 Phe Pro Gly Asn Gly Glu Ser Ala Ala Asp Ser Ala Glu Gly Arg Ala 420 425 430 tcc aag ccg gtc atc gtc gag tcc ccg cag ggc ggc gag tac acc ctc 1644 Ser Lys Pro Val Ile Val Glu Ser Pro Gln Gly Gly Glu Tyr Thr Leu 435 440 445 gac cac ggc atg gtg gcc atc gcc tcc atc acc tcc tgc acc aac acc 1692 Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr Asn Thr 450 455 460 tcc aac ccg tcc gtg ctg gtc gca gcc ggc ctg ctg gcc cgc aag gcc 1740 Ser Asn Pro Ser Val Leu Val Ala Ala Gly Leu Leu Ala Arg Lys Ala 465 470 475 480 aac gag ctg ggc ctg aag tcc aag ccg tgg gtc aag acc atc tgc gcc 1788 Asn Glu Leu Gly Leu Lys Ser Lys Pro Trp Val Lys Thr Ile Cys Ala 485 490 495 ccg ggc tcc cag gtc gtc gac ggt tac ttc aag cgc gcc gac ctc tgg 1836 Pro Gly Ser Gln Val Val Asp Gly Tyr Phe Lys Arg Ala Asp Leu Trp 500 505 510 aag gat ctg gag gcc ctg ggc ttc tac ctc tcc ggc ttc ggc tgc acc 1884 Lys Asp Leu Glu Ala Leu Gly Phe Tyr Leu Ser Gly Phe Gly Cys Thr 515 520 525 tcc tgc atc ggc aac tcc ggt ccg ctc ccg gct gag gtc tcc tcc gcc 1932 Ser Cys Ile Gly Asn Ser Gly Pro Leu Pro Ala Glu Val Ser Ser Ala 530 535 540 atc aac gag cac gac ctg gcc gcc acc gca gtc ctg tcc ggc aac cgt 1980 Ile Asn Glu His Asp Leu Ala Ala Thr Ala Val Leu Ser Gly Asn Arg 545 550 555 560 aac ttc gag ggt cgt atc tcc ccc gac gtc aag atg aac tac ctg gcc 2028 Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr Leu Ala 565 570 575 tcc ccg ccg ctg gtc atc gcc tac gcc atc gcc ggc acc atg gac ttc 2076 Ser Pro Pro Leu Val Ile Ala Tyr Ala Ile Ala Gly Thr Met Asp Phe 580 585 590 gac ttc gag acc gat gcc ctg ggt cag gac aag gac ggc aac gac gtc 2124 Asp Phe Glu Thr Asp Ala Leu Gly Gln Asp Lys Asp Gly Asn Asp Val 595 600 605 ttc ctg aag gac atc tgg ccc tcc acc gag gag atc gag gag acc atc 2172 Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Glu Thr Ile 610 615 620 gcg gcc tcc atc tcc cgc gag ctg tac gag gcc gac tac gct gac gtc 2220 Ala Ala Ser Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala Asp Val 625 630 635 640 ttc aag ggc gac aag cag tgg cag gag ctc gac atc ccg acc ggc aag 2268 Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Ile Pro Thr Gly Lys 645 650 655 acc ttc gag tgg gac gag aac tcc tcc tac atc cgt aag gca ccg tac 2316 Thr Phe Glu Trp Asp Glu Asn Ser Ser Tyr Ile Arg Lys Ala Pro Tyr 660 665 670 ttc gac ggc atg ccg ctc gag ccg cag ccg gtc gag gac atc aag ggc 2364 Phe Asp Gly Met Pro Leu Glu Pro Gln Pro Val Glu Asp Ile Lys Gly 675 680 685 gcc cgc gtc ctc gcc aag ctc ggc gac tcc gtg acc acc gac cac atc 2412 Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp His Ile 690 695 700 tcc ccg gcc tcc tcc atc aag ccg ggc acc ccg gca gcc cag tac ctg 2460 Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln Tyr Leu 705 710 715 720 gac tcc atg ggt gtc gag cgc aag gac tac aac tcc ctg ggc tcc cgt 2508 Asp Ser Met Gly Val Glu Arg Lys Asp Tyr Asn Ser Leu Gly Ser Arg 725 730 735 cgt ggt aac cac gag gtc atg gtc cgc ggt acc ttc gcg aac atc cgc 2556 Arg Gly Asn His Glu Val Met Val Arg Gly Thr Phe Ala Asn Ile Arg 740 745 750 ctg gcc aac cag ctg gtc gac gtc acc ggt ggc tac acc cgt gac ttc 2604 Leu Ala Asn Gln Leu Val Asp Val Thr Gly Gly Tyr Thr Arg Asp Phe 755 760 765 acc cag gag ggt ggc ccg cag gcc tac atc tac gac gcc gcc atg aac 2652 Thr Gln Glu Gly Gly Pro Gln Ala Tyr Ile Tyr Asp Ala Ala Met Asn 770 775 780 tac aag gag gcc ggc acc ccg ctg gtc gtc ctg ggt ggc aag gag tac 2700 Tyr Lys Glu Ala Gly Thr Pro Leu Val Val Leu Gly Gly Lys Glu Tyr 785 790 795 800 ggc acc ggt tcc tcc cgt gac tgg gcc gcg aag ggc acc atc ctg ctc 2748 Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Ile Leu Leu 805 810 815 ggc gtg aag gcc gtc atc acc gag tcc ttc gag cgt atc cac cgc tcg 2796 Gly Val Lys Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His Arg Ser 820 825 830 aac ctg atc ggt atg ggt gtc atc ccg ctg cag ttc ccg gag ggc gag 2844 Asn Leu Ile Gly Met Gly Val Ile Pro Leu Gln Phe Pro Glu Gly Glu 835 840 845 tcc cac gag tcc ctg ggc ctg gac ggc acc gag gtc ttc gac atc gag 2892 Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Val Phe Asp Ile Glu 850 855 860 ggc atc acc gcc ctg aac gag ggc gag acc ccg aag acc gtc aag gtc 2940 Gly Ile Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val Lys Val 865 870 875 880 acc gcc acc aag gag gac ggc tcc acc gtg gag ttc gac gcg att gtc 2988 Thr Ala Thr Lys Glu Asp Gly Ser Thr Val Glu Phe Asp Ala Ile Val 885 890 895 cgc atc gac acc ccg ggt gag gcc gac tac tac cgc aac ggc ggc atc 3036 Arg Ile Asp Thr Pro Gly Glu Ala Asp Tyr Tyr Arg Asn Gly Gly Ile 900 905 910 ctg cag tac gtc ctg cgt cag atg atc gct gct gcc aag taa 3078 Leu Gln Tyr Val Leu Arg Gln Met Ile Ala Ala Ala Lys 915 920 925 gcgagcatcc tcccgcagtg tgaactgcct ctgagagggg gctgcatccc cggctaaggc 3138 gtcacgccac cggggagagc agccccctct cccctttttc cctattcccc cgacaggagt 3198 cccctcatgc ccgtggtcag cgacaccgag ttgacgcgtc gtcagaccga gatcctcgaa 3258 ggcgcgcggc gttgcttcgc cgagtacggc tacgagggcg ccacggtgcg ccgcctcgag 3318 gaggccacgg gcaagtcgcg gggtgcgatc ttccaccact tcggtgacaa ggagaacctc 3378 <210> 134 <211> 925 <212> PRT <213> Corynebacterium humireducens <400> 134 Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Arg Thr Leu Glu Val 1 5 10 15 Gly Glu Lys Ser Tyr Asp Tyr Phe Ala Leu Asp Ala Val Pro Gly Met 20 25 30 Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg 35 40 45 Thr Glu Asp Gly Ala Asn Ile Thr Thr Glu His Ile Glu Ala Ile Ala 50 55 60 Asn Trp Asp Pro Lys Ala Glu Pro Ser Ile Glu Ile Gln Phe Thr Pro 65 70 75 80 Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Ala Cys Ala Val Asp 85 90 95 Leu Ala Thr Met Arg Glu Ala Val Lys Thr Leu Gly Gly Asp Pro Asp 100 105 110 Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val 115 120 125 Ile Val Glu Ala Phe Gly Thr Ser Ala Ala Leu Glu Lys Asn Val Glu 130 135 140 Ile Glu Tyr Gln Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly 145 150 155 160 Ala Glu Asn Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile 165 170 175 Val His Gln Val Asn Ile Glu Tyr Leu Ser Arg Val Val Phe Asp Asn 180 185 190 Asn Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr 195 200 205 Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile 210 215 220 Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro 225 230 235 240 Arg Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro Ala Gly Val Thr 245 250 255 Ala Thr Asp Val Val Leu Thr Ile Thr Glu Met Leu Arg Asn His Gly 260 265 270 Val Val Gln Lys Phe Val Glu Phe Tyr Gly Asn Gly Val Lys Ser Val 275 280 285 Pro Leu Ala Asn Arg Ala Thr Ile Gly Asn Met Ser Pro Glu Phe Gly 290 295 300 Ser Thr Cys Ala Met Phe Pro Ile Asp Glu Glu Thr Ile Lys Tyr Leu 305 310 315 320 Glu Leu Thr Gly Arg Ser Glu Glu Asp Ile Ala Arg Val Glu Ala Tyr 325 330 335 Ala Lys Ala Gln Gly Met Trp Leu Tyr Glu Asp Ala Pro Glu Ala Glu 340 345 350 Tyr Ser Glu Tyr Leu Glu Leu Asp Leu Ser Thr Val Val Pro Ser Ile 355 360 365 Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu Leu Ser Glu Ala Lys 370 375 380 Ala Gln Phe Arg Lys Asp Leu Pro Thr Tyr Ala Ser Asp Glu Val Ala 385 390 395 400 Glu Pro Val Arg Ala Thr Lys Ala Val Gly Asp Asp Tyr Asn Gln Ser 405 410 415 Phe Pro Gly Asn Gly Glu Ser Ala Ala Asp Ser Ala Glu Gly Arg Ala 420 425 430 Ser Lys Pro Val Ile Val Glu Ser Pro Gln Gly Gly Glu Tyr Thr Leu 435 440 445 Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys Thr Asn Thr 450 455 460 Ser Asn Pro Ser Val Leu Val Ala Ala Gly Leu Leu Ala Arg Lys Ala 465 470 475 480 Asn Glu Leu Gly Leu Lys Ser Lys Pro Trp Val Lys Thr Ile Cys Ala 485 490 495 Pro Gly Ser Gln Val Val Asp Gly Tyr Phe Lys Arg Ala Asp Leu Trp 500 505 510 Lys Asp Leu Glu Ala Leu Gly Phe Tyr Leu Ser Gly Phe Gly Cys Thr 515 520 525 Ser Cys Ile Gly Asn Ser Gly Pro Leu Pro Ala Glu Val Ser Ser Ala 530 535 540 Ile Asn Glu His Asp Leu Ala Ala Thr Ala Val Leu Ser Gly Asn Arg 545 550 555 560 Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn Tyr Leu Ala 565 570 575 Ser Pro Pro Leu Val Ile Ala Tyr Ala Ile Ala Gly Thr Met Asp Phe 580 585 590 Asp Phe Glu Thr Asp Ala Leu Gly Gln Asp Lys Asp Gly Asn Asp Val 595 600 605 Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu Glu Thr Ile 610 615 620 Ala Ala Ser Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr Ala Asp Val 625 630 635 640 Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Ile Pro Thr Gly Lys 645 650 655 Thr Phe Glu Trp Asp Glu Asn Ser Ser Tyr Ile Arg Lys Ala Pro Tyr 660 665 670 Phe Asp Gly Met Pro Leu Glu Pro Gln Pro Val Glu Asp Ile Lys Gly 675 680 685 Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr Asp His Ile 690 695 700 Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala Gln Tyr Leu 705 710 715 720 Asp Ser Met Gly Val Glu Arg Lys Asp Tyr Asn Ser Leu Gly Ser Arg 725 730 735 Arg Gly Asn His Glu Val Met Val Arg Gly Thr Phe Ala Asn Ile Arg 740 745 750 Leu Ala Asn Gln Leu Val Asp Val Thr Gly Gly Tyr Thr Arg Asp Phe 755 760 765 Thr Gln Glu Gly Gly Pro Gln Ala Tyr Ile Tyr Asp Ala Ala Met Asn 770 775 780 Tyr Lys Glu Ala Gly Thr Pro Leu Val Val Leu Gly Gly Lys Glu Tyr 785 790 795 800 Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr Ile Leu Leu 805 810 815 Gly Val Lys Ala Val Ile Thr Glu Ser Phe Glu Arg Ile His Arg Ser 820 825 830 Asn Leu Ile Gly Met Gly Val Ile Pro Leu Gln Phe Pro Glu Gly Glu 835 840 845 Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Val Phe Asp Ile Glu 850 855 860 Gly Ile Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr Val Lys Val 865 870 875 880 Thr Ala Thr Lys Glu Asp Gly Ser Thr Val Glu Phe Asp Ala Ile Val 885 890 895 Arg Ile Asp Thr Pro Gly Glu Ala Asp Tyr Tyr Arg Asn Gly Gly Ile 900 905 910 Leu Gln Tyr Val Leu Arg Gln Met Ile Ala Ala Ala Lys 915 920 925 <210> 135 <211> 1752 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1452) <223> gene citrate synthase <400> 135 agctgctctc cgcccagcag ctcgaggagc tcggcttcaa cgccgtcatc tacccggtga 60 ccaccctgcg catcgccatg ggacaggtcg aggaggctct cgcggagatc gccgccaccg 120 gcacccagac cgggtgggtc gaccgcatgc agcaccgctc ccgcctctac gaactgctcc 180 gctacaacga gtacaacgcc ttcgaccagg aggttttcac gtactccgcc gacacctacc 240 agcccacctt ccagtagccc accagcaact cctgacattc cttgaaaggg gatgagtacc 300 atg tcc gac aac acc aac atc gag gtc cgc aag ggt ctc tac ggc gtg 348 Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val 1 5 10 15 atc gcc gac tac acc gca gtc tcc aag gtc atg ccg gag acc aac tcc 396 Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser 20 25 30 ctg acc tac cgc gga tac gcc gtc cag gac ctc gtc gca gag tgc tcc 444 Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser 35 40 45 ttc gag gag gtc ttc tac ctc ctg tgg aac ggt gag ctg ccg aac gag 492 Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu 50 55 60 gag cag ctg gcg gag ttc aac agg cgc ggc cgt tcc tac cgc aag ctc 540 Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu 65 70 75 80 gac gcc ggc ctg atc gca ctc atc cac tcc ctg ccg ctc gac tgt cac 588 Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His 85 90 95 ccg atg gac gtc atg cgc acc gcc gtg tcc tac atg ggc acc aag gac 636 Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp 100 105 110 tcc gag cac ttc acc ccg gac gcc gac cac atc acc cac gtc ggc cac 684 Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His 115 120 125 aac ctc ctc gcc cag ctg ccg atg gtg ctg gcc atg gac atc cgc cgc 732 Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg 130 135 140 cgt cag ggc ctc gac atc gtc gcc ccg gac ccg gac aag tcc gtc gcc 780 Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala 145 150 155 160 cac aac ctg ctg tcg atg gtc ttc ggc aac ggc ccg gag tcc ccg gcg 828 His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala 165 170 175 tcc aac ccg gac gac gtg cgt gac ttc gag aag tcg ctc atc ctc tac 876 Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr 180 185 190 gcc gag cac tcc ttc aac gcc tcg acc ttc acc tcg cgt gtg atc acc 924 Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr 195 200 205 tcg acc cgc tcg gac gtc tac tcc gcg atc acc ggt gcc atc ggc gcc 972 Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala 210 215 220 ctc aag ggc ccg ctg cac ggc ggc gcc aac gag ttc gtc atg cac acc 1020 Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr 225 230 235 240 atg ctg gag atc gac gac ccg gag aag gcc gcc gac tgg gtc aac aac 1068 Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn 245 250 255 gcc ctg gac aac aag gac ctc atc atg ggc ttc ggt cac cgc gtg tac 1116 Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr 260 265 270 aag aag ggt gac tcg cgt gtc ccg tcc atg gag gcg tcc ttc cgt gac 1164 Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp 275 280 285 ctc gcc gag cgt cac gac ggc gcc aag tgg gtc gcc atg tac gag aac 1212 Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn 290 295 300 atg cgc gac gcc atg gac gcc cgc acc ggc atc aag ccg aac ctg gac 1260 Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp 305 310 315 320 ttc ccg gcc ggc ccg gcg tac cac ctg ctc ggc ttc ccg gtc gac ttc 1308 Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe 325 330 335 ttc acc ccg ctg ttc gtc gtc gcc cgc atc gcc ggc tgg acc gcc cac 1356 Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His 340 345 350 atc gtg gag cag tac aac gac aac tcc ctg att cgt ccg ctc tcc gcc 1404 Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala 355 360 365 tac aac ggc ccc gag cag cgc cag gtc acc ccg ctg gcc gcc cgt tag 1452 Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg 370 375 380 ccccacggcc ggcaccggac caccgcgacg cccacggcac tgcccacaca ttggggtggt 1512 gcgcgtgggt ttcgctgttc ggaacacctg tcggcccatt ccgcagggcg ggacagaaac 1572 ggcctttcgg gcgcccgtcc cgcggagtgc ggaaatggac cgcaccgggt ccaccacctg 1632 cagatacccc ctgtgactag gggatactcc cccgaagaca accctctttc ttgcgatact 1692 gtggtgtgcc agagagctca cccgctctca gacacacccc cgacatgacg ttcggaaagg 1752 <210> 136 <211> 383 <212> PRT <213> Corynebacterium humireducens <400> 136 Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val 1 5 10 15 Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser 20 25 30 Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser 35 40 45 Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu 50 55 60 Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu 65 70 75 80 Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His 85 90 95 Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp 100 105 110 Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His 115 120 125 Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg 130 135 140 Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala 145 150 155 160 His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala 165 170 175 Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr 180 185 190 Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr 195 200 205 Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala 210 215 220 Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr 225 230 235 240 Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn 245 250 255 Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr 260 265 270 Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp 275 280 285 Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn 290 295 300 Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp 305 310 315 320 Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe 325 330 335 Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His 340 345 350 Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala 355 360 365 Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg 370 375 380 <210> 137 <211> 3372 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(3072) <223> gene pepc <400> 137 cggttccgtc aagaccgact cggtcgccga gatcgtcggt cagcccgacg tcgacggtgg 60 tctcgtcggt ggcgcctcgc tcgacggcga ggagttcgcc aagctggcgg ccgccgcggc 120 gtccgtcgtc tagcatcacc tcatccaccg gccccgggca ctgaacattc agtgcccggg 180 gccggtgtcc tttctccggg gaatatggtg cgcaatcccc acgcacggcc gtcatatgcg 240 tcatgatggg gaggcgaacc gcatccccgc cttcttcgag tacgaacagg gagacactca 300 atg acc gtc cgc gat cat ctg cag gaa gac atc cgc tac ctc ggc cgt 348 Met Thr Val Arg Asp His Leu Gln Glu Asp Ile Arg Tyr Leu Gly Arg 1 5 10 15 atc ctc ggc cag gtc atc gcg gaa cag gag ggc gag gac gtg ttc aac 396 Ile Leu Gly Gln Val Ile Ala Glu Gln Glu Gly Glu Asp Val Phe Asn 20 25 30 ctc gtc gag cac gcc cgc cag cag gcc ttc gag gtg gcc aag ggc aac 444 Leu Val Glu His Ala Arg Gln Gln Ala Phe Glu Val Ala Lys Gly Asn 35 40 45 gcc tcc ctg gag gtg ctc gtc gac ctg ttc cgg aac atc gag ccg gag 492 Ala Ser Leu Glu Val Leu Val Asp Leu Phe Arg Asn Ile Glu Pro Glu 50 55 60 cgc gcg acc ccg gtg atc cgg gcg ttc agc cac ttc gcc ctg atg gcg 540 Arg Ala Thr Pro Val Ile Arg Ala Phe Ser His Phe Ala Leu Met Ala 65 70 75 80 aac ctc gcg gag gac atc cac gac gac ttc cac cgg gag cgc atc ctc 588 Asn Leu Ala Glu Asp Ile His Asp Asp Phe His Arg Glu Arg Ile Leu 85 90 95 gat gag ggc ggg gcc ccc gac tcc acc ctc aac gcc acg tgg gag aag 636 Asp Glu Gly Gly Ala Pro Asp Ser Thr Leu Asn Ala Thr Trp Glu Lys 100 105 110 ttc cgg gag gcc ggc gtc agt gcc gcg gac atc gag agg tcg ctg tct 684 Phe Arg Glu Ala Gly Val Ser Ala Ala Asp Ile Glu Arg Ser Leu Ser 115 120 125 gcc ggg ctc gtc gcc ccg gtg ctc acc gcc cac ccg acg gag acc cgc 732 Ala Gly Leu Val Ala Pro Val Leu Thr Ala His Pro Thr Glu Thr Arg 130 135 140 cgc cgc acc gtc ttc gac gcc cag aag cac atc acc gag ctc atg ctg 780 Arg Arg Thr Val Phe Asp Ala Gln Lys His Ile Thr Glu Leu Met Leu 145 150 155 160 cag cgg cac gcc gtc cag gac gcg gag ccg aac gcc cgc acc gag gac 828 Gln Arg His Ala Val Gln Asp Ala Glu Pro Asn Ala Arg Thr Glu Asp 165 170 175 cgt ctc gcg gag atc gag cgc aac atc cgc cgc cgc atg acc atc ctg 876 Arg Leu Ala Glu Ile Glu Arg Asn Ile Arg Arg Arg Met Thr Ile Leu 180 185 190 tgg cag acc gcg ctc atc cgg cag gcc cgc ccc cgc atc gag gac gag 924 Trp Gln Thr Ala Leu Ile Arg Gln Ala Arg Pro Arg Ile Glu Asp Glu 195 200 205 atc gag gtc ggc ctg cgc tac tac acg ctc tcc ctg ctc cgg gag atc 972 Ile Glu Val Gly Leu Arg Tyr Tyr Thr Leu Ser Leu Leu Arg Glu Ile 210 215 220 ccg gcg ctc aac cgg cac gtc gtc gac acg ctc acg gag cgt ttc ggc 1020 Pro Ala Leu Asn Arg His Val Val Asp Thr Leu Thr Glu Arg Phe Gly 225 230 235 240 gcc gac ctg cgc aac agc gcc ggt cag gcc atc gtc cgc ccg ggc tcc 1068 Ala Asp Leu Arg Asn Ser Ala Gly Gln Ala Ile Val Arg Pro Gly Ser 245 250 255 tgg atc ggc ggc gac cac gac ggc aac ccc ttc gtc acc gcc gac aca 1116 Trp Ile Gly Gly Asp His Asp Gly Asn Pro Phe Val Thr Ala Asp Thr 260 265 270 ctg gac tac gct tcc cga cgc gcc gcc cag acc gtc ctc aag tac tac 1164 Leu Asp Tyr Ala Ser Arg Arg Ala Ala Gln Thr Val Leu Lys Tyr Tyr 275 280 285 gtc acg cag ctc cac gcc ctc gag cac gag ctg agc ctc tcc gac cgc 1212 Val Thr Gln Leu His Ala Leu Glu His Glu Leu Ser Leu Ser Asp Arg 290 295 300 atg acc tcc gtc acc gtg gag ctc gtc gcc ctc gcc ggg cgg gga aag 1260 Met Thr Ser Val Thr Val Glu Leu Val Ala Leu Ala Gly Arg Gly Lys 305 310 315 320 aac gac gtc ccc tcg cgt gtc gac gag ccc tac cgc cgc gcc gtc cac 1308 Asn Asp Val Pro Ser Arg Val Asp Glu Pro Tyr Arg Arg Ala Val His 325 330 335 ggc gtc cgg ggc cgc atc ctc gcc acg acc gcg gcg ctc atc ggg gag 1356 Gly Val Arg Gly Arg Ile Leu Ala Thr Thr Ala Ala Leu Ile Gly Glu 340 345 350 gac gcc gtc gag ggc gtc tgg cac cgc gag cat gag ccc tac ggc tcc 1404 Asp Ala Val Glu Gly Val Trp His Arg Glu His Glu Pro Tyr Gly Ser 355 360 365 ccg cag gag ttc gag gcg gac ctc agg gtc atc gac acc tcg ctg cgt 1452 Pro Gln Glu Phe Glu Ala Asp Leu Arg Val Ile Asp Thr Ser Leu Arg 370 375 380 gcc tcc cac gac gag atc atc gcc gac gac cgt ctc agc tcc atc cgg 1500 Ala Ser His Asp Glu Ile Ile Ala Asp Asp Arg Leu Ser Ser Ile Arg 385 390 395 400 gcg gcc gtc gcc tcc ttc ggg ttc cac ctg tac tcg atc gac ctg cga 1548 Ala Ala Val Ala Ser Phe Gly Phe His Leu Tyr Ser Ile Asp Leu Arg 405 410 415 cag aac tcc gag tcc ttc gag aac gtc ctc acc gag gtc ttc gcc acc 1596 Gln Asn Ser Glu Ser Phe Glu Asn Val Leu Thr Glu Val Phe Ala Thr 420 425 430 gcg cac gtc cac ccc aac tac gac acg ctg cgg gag gag gtg aag atc 1644 Ala His Val His Pro Asn Tyr Asp Thr Leu Arg Glu Glu Val Lys Ile 435 440 445 gag ctg ctc acc cgc gaa ctg cag acc ccg cgc ccc ctc gtc ccg cgc 1692 Glu Leu Leu Thr Arg Glu Leu Gln Thr Pro Arg Pro Leu Val Pro Arg 450 455 460 ggc tac cgg ggc ttc tcc gag ccg acc cag cgg gag ctc gac ctc atc 1740 Gly Tyr Arg Gly Phe Ser Glu Pro Thr Gln Arg Glu Leu Asp Leu Ile 465 470 475 480 tcc cag gcc gcg gac tcc gtc gcc cgc ttc ggc gag cag atg atc ccg 1788 Ser Gln Ala Ala Asp Ser Val Ala Arg Phe Gly Glu Gln Met Ile Pro 485 490 495 cac cag atc atc tcc atg gcg cag tcg gtc agc gac atc ctc gag ccg 1836 His Gln Ile Ile Ser Met Ala Gln Ser Val Ser Asp Ile Leu Glu Pro 500 505 510 atg gtg ctg ctc aag gag gtc ggc ctc atc cag gcc aac ggc cag ggc 1884 Met Val Leu Leu Lys Glu Val Gly Leu Ile Gln Ala Asn Gly Gln Gly 515 520 525 ccg acc ggc agc gtc gac atc atc ccg ctg ttc gag acg atc gac gac 1932 Pro Thr Gly Ser Val Asp Ile Ile Pro Leu Phe Glu Thr Ile Asp Asp 530 535 540 ctc cag gcc ggc gcc ggc atc ctc cgt gag ctg tgg gac ctg ccg atc 1980 Leu Gln Ala Gly Ala Gly Ile Leu Arg Glu Leu Trp Asp Leu Pro Ile 545 550 555 560 tac cgg gcg tac ctc gcc cag cgc ggc gac atc cag gag gtc atg ctc 2028 Tyr Arg Ala Tyr Leu Ala Gln Arg Gly Asp Ile Gln Glu Val Met Leu 565 570 575 ggc tac tcg gac tcc aac aag gac ggc ggc tac ttc gcc gcc aac tgg 2076 Gly Tyr Ser Asp Ser Asn Lys Asp Gly Gly Tyr Phe Ala Ala Asn Trp 580 585 590 gcg ctc tac gac gcc gag acc gac ctg gtg aag gtc ggc cgc gag tac 2124 Ala Leu Tyr Asp Ala Glu Thr Asp Leu Val Lys Val Gly Arg Glu Tyr 595 600 605 ggt gtg cgc ctg cga ctc ttc cac ggc cgc ggc ggc acc gtc ggc cgt 2172 Gly Val Arg Leu Arg Leu Phe His Gly Arg Gly Gly Thr Val Gly Arg 610 615 620 ggt ggc ggc ccc tcc tac gac gcg ctc ctc gcc cag ccg cag ggg gcg 2220 Gly Gly Gly Pro Ser Tyr Asp Ala Leu Leu Ala Gln Pro Gln Gly Ala 625 630 635 640 gtc gac ggt tcc gtg cgc atc acc gag cag ggc gag atc atc tcg gcc 2268 Val Asp Gly Ser Val Arg Ile Thr Glu Gln Gly Glu Ile Ile Ser Ala 645 650 655 aag tac ggc tcc ccg cgt acg gcc cgc cgc aac ctg gag gca ctg gtc 2316 Lys Tyr Gly Ser Pro Arg Thr Ala Arg Arg Asn Leu Glu Ala Leu Val 660 665 670 tcc gcg acg ctc gag gcc agc ctg ctc acc gtc gac gac ctc gcc gac 2364 Ser Ala Thr Leu Glu Ala Ser Leu Leu Thr Val Asp Asp Leu Ala Asp 675 680 685 cgg gcc cgc gcg acg cag ata atg agc gaa ctc gcg cag atc tcc cgc 2412 Arg Ala Arg Ala Thr Gln Ile Met Ser Glu Leu Ala Gln Ile Ser Arg 690 695 700 cgc aag tac tcc gag ctc gtc cac gag gac ccg ggg ttc atc ccg tac 2460 Arg Lys Tyr Ser Glu Leu Val His Glu Asp Pro Gly Phe Ile Pro Tyr 705 710 715 720 ttc acc cag tcg acc ccg ctc gac gag atc ggc tcc ctc aac atc ggc 2508 Phe Thr Gln Ser Thr Pro Leu Asp Glu Ile Gly Ser Leu Asn Ile Gly 725 730 735 tcc cgc ccg acc gcc cgc aag cag acc aag ggc gtc gac gat ctc cgc 2556 Ser Arg Pro Thr Ala Arg Lys Gln Thr Lys Gly Val Asp Asp Leu Arg 740 745 750 gcc atc ccg tgg gtg ctc gcc tgg tcc cag tcg cgt gtg ctg ctg ccg 2604 Ala Ile Pro Trp Val Leu Ala Trp Ser Gln Ser Arg Val Leu Leu Pro 755 760 765 ggc tgg ttc ggc gtc ggc acg gcc ctg gcc gag tgg atc ggg gag ggg 2652 Gly Trp Phe Gly Val Gly Thr Ala Leu Ala Glu Trp Ile Gly Glu Gly 770 775 780 gag gac agg gac gag cgc atc gcc gag ctg cgg gag ctc tac gag tcc 2700 Glu Asp Arg Asp Glu Arg Ile Ala Glu Leu Arg Glu Leu Tyr Glu Ser 785 790 795 800 tgg ccc ttc ttc acc tcg atc atg tcc aac atg gcg cag gtc atg tcg 2748 Trp Pro Phe Phe Thr Ser Ile Met Ser Asn Met Ala Gln Val Met Ser 805 810 815 aag gcg ggc atg gat ctc gcg gag ctc tac gcc cgg ctc atc gac gac 2796 Lys Ala Gly Met Asp Leu Ala Glu Leu Tyr Ala Arg Leu Ile Asp Asp 820 825 830 cgg gag gtc gcc gag cgc gtc cac ggt gtc atc acg gag gag ttc gag 2844 Arg Glu Val Ala Glu Arg Val His Gly Val Ile Thr Glu Glu Phe Glu 835 840 845 ctc acc cgc gag atg ttc agc acc gtc acc ggc agc gcc gag ctg ctc 2892 Leu Thr Arg Glu Met Phe Ser Thr Val Thr Gly Ser Ala Glu Leu Leu 850 855 860 gcc gac aac ccg gcg ctg gcc cgt tcg gtg cgc agg cgc ttc ccg tac 2940 Ala Asp Asn Pro Ala Leu Ala Arg Ser Val Arg Arg Arg Phe Pro Tyr 865 870 875 880 ctg ctg ccg ctc aac atc atc cag ctg gag ctg ctg cgc cgc cac cgt 2988 Leu Leu Pro Leu Asn Ile Ile Gln Leu Glu Leu Leu Arg Arg His Arg 885 890 895 gcc ggc gac tcc cga cgc gcc gtg tcc cgc ggc atc cag ctg acg atg 3036 Ala Gly Asp Ser Arg Arg Ala Val Ser Arg Gly Ile Gln Leu Thr Met 900 905 910 aac gga ctc gcc acc gca ctg cgc aac tcc ggg tag acctggtgta 3082 Asn Gly Leu Ala Thr Ala Leu Arg Asn Ser Gly 915 920 gactgagccg gagtttgtgc cgcccgattc ccgcaaagga ccttcatgac cctcgcactc 3142 cagatcatcc tcgtgctcac cgccgtgctc atgacggtgt tcgttctgct gcacaagggt 3202 aagggcggcg gcctgtccag cctcttcggt ggcggcatgc agtcgaacct ctcgggttcg 3262 acggtcgtcg agaagaacct cgaccgctac acgatcctca tggccgtcat ctggatcgcc 3322 tgcatcgtgg cgctgaacct catccaggct ttcgccggct aggttcaatg 3372 <210> 138 <211> 923 <212> PRT <213> Corynebacterium humireducens <400> 138 Met Thr Val Arg Asp His Leu Gln Glu Asp Ile Arg Tyr Leu Gly Arg 1 5 10 15 Ile Leu Gly Gln Val Ile Ala Glu Gln Glu Gly Glu Asp Val Phe Asn 20 25 30 Leu Val Glu His Ala Arg Gln Gln Ala Phe Glu Val Ala Lys Gly Asn 35 40 45 Ala Ser Leu Glu Val Leu Val Asp Leu Phe Arg Asn Ile Glu Pro Glu 50 55 60 Arg Ala Thr Pro Val Ile Arg Ala Phe Ser His Phe Ala Leu Met Ala 65 70 75 80 Asn Leu Ala Glu Asp Ile His Asp Asp Phe His Arg Glu Arg Ile Leu 85 90 95 Asp Glu Gly Gly Ala Pro Asp Ser Thr Leu Asn Ala Thr Trp Glu Lys 100 105 110 Phe Arg Glu Ala Gly Val Ser Ala Ala Asp Ile Glu Arg Ser Leu Ser 115 120 125 Ala Gly Leu Val Ala Pro Val Leu Thr Ala His Pro Thr Glu Thr Arg 130 135 140 Arg Arg Thr Val Phe Asp Ala Gln Lys His Ile Thr Glu Leu Met Leu 145 150 155 160 Gln Arg His Ala Val Gln Asp Ala Glu Pro Asn Ala Arg Thr Glu Asp 165 170 175 Arg Leu Ala Glu Ile Glu Arg Asn Ile Arg Arg Arg Met Thr Ile Leu 180 185 190 Trp Gln Thr Ala Leu Ile Arg Gln Ala Arg Pro Arg Ile Glu Asp Glu 195 200 205 Ile Glu Val Gly Leu Arg Tyr Tyr Thr Leu Ser Leu Leu Arg Glu Ile 210 215 220 Pro Ala Leu Asn Arg His Val Val Asp Thr Leu Thr Glu Arg Phe Gly 225 230 235 240 Ala Asp Leu Arg Asn Ser Ala Gly Gln Ala Ile Val Arg Pro Gly Ser 245 250 255 Trp Ile Gly Gly Asp His Asp Gly Asn Pro Phe Val Thr Ala Asp Thr 260 265 270 Leu Asp Tyr Ala Ser Arg Arg Ala Ala Gln Thr Val Leu Lys Tyr Tyr 275 280 285 Val Thr Gln Leu His Ala Leu Glu His Glu Leu Ser Leu Ser Asp Arg 290 295 300 Met Thr Ser Val Thr Val Glu Leu Val Ala Leu Ala Gly Arg Gly Lys 305 310 315 320 Asn Asp Val Pro Ser Arg Val Asp Glu Pro Tyr Arg Arg Ala Val His 325 330 335 Gly Val Arg Gly Arg Ile Leu Ala Thr Thr Ala Ala Leu Ile Gly Glu 340 345 350 Asp Ala Val Glu Gly Val Trp His Arg Glu His Glu Pro Tyr Gly Ser 355 360 365 Pro Gln Glu Phe Glu Ala Asp Leu Arg Val Ile Asp Thr Ser Leu Arg 370 375 380 Ala Ser His Asp Glu Ile Ile Ala Asp Asp Arg Leu Ser Ser Ile Arg 385 390 395 400 Ala Ala Val Ala Ser Phe Gly Phe His Leu Tyr Ser Ile Asp Leu Arg 405 410 415 Gln Asn Ser Glu Ser Phe Glu Asn Val Leu Thr Glu Val Phe Ala Thr 420 425 430 Ala His Val His Pro Asn Tyr Asp Thr Leu Arg Glu Glu Val Lys Ile 435 440 445 Glu Leu Leu Thr Arg Glu Leu Gln Thr Pro Arg Pro Leu Val Pro Arg 450 455 460 Gly Tyr Arg Gly Phe Ser Glu Pro Thr Gln Arg Glu Leu Asp Leu Ile 465 470 475 480 Ser Gln Ala Ala Asp Ser Val Ala Arg Phe Gly Glu Gln Met Ile Pro 485 490 495 His Gln Ile Ile Ser Met Ala Gln Ser Val Ser Asp Ile Leu Glu Pro 500 505 510 Met Val Leu Leu Lys Glu Val Gly Leu Ile Gln Ala Asn Gly Gln Gly 515 520 525 Pro Thr Gly Ser Val Asp Ile Ile Pro Leu Phe Glu Thr Ile Asp Asp 530 535 540 Leu Gln Ala Gly Ala Gly Ile Leu Arg Glu Leu Trp Asp Leu Pro Ile 545 550 555 560 Tyr Arg Ala Tyr Leu Ala Gln Arg Gly Asp Ile Gln Glu Val Met Leu 565 570 575 Gly Tyr Ser Asp Ser Asn Lys Asp Gly Gly Tyr Phe Ala Ala Asn Trp 580 585 590 Ala Leu Tyr Asp Ala Glu Thr Asp Leu Val Lys Val Gly Arg Glu Tyr 595 600 605 Gly Val Arg Leu Arg Leu Phe His Gly Arg Gly Gly Thr Val Gly Arg 610 615 620 Gly Gly Gly Pro Ser Tyr Asp Ala Leu Leu Ala Gln Pro Gln Gly Ala 625 630 635 640 Val Asp Gly Ser Val Arg Ile Thr Glu Gln Gly Glu Ile Ile Ser Ala 645 650 655 Lys Tyr Gly Ser Pro Arg Thr Ala Arg Arg Asn Leu Glu Ala Leu Val 660 665 670 Ser Ala Thr Leu Glu Ala Ser Leu Leu Thr Val Asp Asp Leu Ala Asp 675 680 685 Arg Ala Arg Ala Thr Gln Ile Met Ser Glu Leu Ala Gln Ile Ser Arg 690 695 700 Arg Lys Tyr Ser Glu Leu Val His Glu Asp Pro Gly Phe Ile Pro Tyr 705 710 715 720 Phe Thr Gln Ser Thr Pro Leu Asp Glu Ile Gly Ser Leu Asn Ile Gly 725 730 735 Ser Arg Pro Thr Ala Arg Lys Gln Thr Lys Gly Val Asp Asp Leu Arg 740 745 750 Ala Ile Pro Trp Val Leu Ala Trp Ser Gln Ser Arg Val Leu Leu Pro 755 760 765 Gly Trp Phe Gly Val Gly Thr Ala Leu Ala Glu Trp Ile Gly Glu Gly 770 775 780 Glu Asp Arg Asp Glu Arg Ile Ala Glu Leu Arg Glu Leu Tyr Glu Ser 785 790 795 800 Trp Pro Phe Phe Thr Ser Ile Met Ser Asn Met Ala Gln Val Met Ser 805 810 815 Lys Ala Gly Met Asp Leu Ala Glu Leu Tyr Ala Arg Leu Ile Asp Asp 820 825 830 Arg Glu Val Ala Glu Arg Val His Gly Val Ile Thr Glu Glu Phe Glu 835 840 845 Leu Thr Arg Glu Met Phe Ser Thr Val Thr Gly Ser Ala Glu Leu Leu 850 855 860 Ala Asp Asn Pro Ala Leu Ala Arg Ser Val Arg Arg Arg Phe Pro Tyr 865 870 875 880 Leu Leu Pro Leu Asn Ile Ile Gln Leu Glu Leu Leu Arg Arg His Arg 885 890 895 Ala Gly Asp Ser Arg Arg Ala Val Ser Arg Gly Ile Gln Leu Thr Met 900 905 910 Asn Gly Leu Ala Thr Ala Leu Arg Asn Ser Gly 915 920 <210> 139 <211> 2346 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2046) <223> gene pyruvate dehydrogenase <400> 139 cggtcgccga gcgtgcccgt gccgagcatg agcccggcca gcacgagggg gtaggcgttg 60 atgatccaca gcgcctgcac ctcggaggtg tggagctgtc tgcgcagctc ggggagcgcg 120 gtgaagagga tcgagttgtc caccccgacc atcagcagcc ccagggagat gatggcgagg 180 aaggcccacc gctgggccgg ggtggagtcg acgttgtcga ggtgcgtcac gcagcggaac 240 tgtaccgacc gttcgttaca gtttcaacgc ggggcatgaa tcctccgtac gctcgggacc 300 atg gca cgc acc tac gca cag cag ctc gtc gac gcc ctc gag agg cag 348 Met Ala Arg Thr Tyr Ala Gln Gln Leu Val Asp Ala Leu Glu Arg Gln 1 5 10 15 ggc gtg gaa cgg atc tac gga gtc gtc ggg gac agc ctc aac ccc atc 396 Gly Val Glu Arg Ile Tyr Gly Val Val Gly Asp Ser Leu Asn Pro Ile 20 25 30 gtc gac gcc gtc cgc acc tcc ccg atc gag tgg atc cac gtc cgc aac 444 Val Asp Ala Val Arg Thr Ser Pro Ile Glu Trp Ile His Val Arg Asn 35 40 45 gag gag gcc gcc gcc ttc gcc gcc ggc gcc gag tcc ctc ctc acc ggc 492 Glu Glu Ala Ala Ala Phe Ala Ala Gly Ala Glu Ser Leu Leu Thr Gly 50 55 60 cgc ctc gcc gtg tgc gcg ggc tcc tgc ggc ccc ggc aac ctc cac ctc 540 Arg Leu Ala Val Cys Ala Gly Ser Cys Gly Pro Gly Asn Leu His Leu 65 70 75 80 atc cag ggt ctc tac gac gcc cac cgc aac ggc gcc cac gtc ctc gcc 588 Ile Gln Gly Leu Tyr Asp Ala His Arg Asn Gly Ala His Val Leu Ala 85 90 95 ctg gcc tcc cac atc ccc agc aac cag atc ggt acc tca tac ttc cag 636 Leu Ala Ser His Ile Pro Ser Asn Gln Ile Gly Thr Ser Tyr Phe Gln 100 105 110 gag acc cac ccc gaa cag ctc ttc gcg gaa tgt tcc ggt tac tgc gag 684 Glu Thr His Pro Glu Gln Leu Phe Ala Glu Cys Ser Gly Tyr Cys Glu 115 120 125 atg gtc aac tcc gcc gac cag ggc gcc cgc gtc ctg cac cac gcg atc 732 Met Val Asn Ser Ala Asp Gln Gly Ala Arg Val Leu His His Ala Ile 130 135 140 cag tcg acg atg gcc ggg cag ggc gtg tcc gtg ctg gtc atc ccg ggg 780 Gln Ser Thr Met Ala Gly Gln Gly Val Ser Val Leu Val Ile Pro Gly 145 150 155 160 gac atc gcc acg cag gac gcc ggc gac gac cgg ttc ctg cag tcc acc 828 Asp Ile Ala Thr Gln Asp Ala Gly Asp Asp Arg Phe Leu Gln Ser Thr 165 170 175 gtc gcc acc ggc cgc ccc gtg ctc cac ccc gac gcc cgc gag gcc gcc 876 Val Ala Thr Gly Arg Pro Val Leu His Pro Asp Ala Arg Glu Ala Ala 180 185 190 gcc ctg gtc gcg gcc atc aac gcc gcc gac acg gtg acc ctg ttc tgc 924 Ala Leu Val Ala Ala Ile Asn Ala Ala Asp Thr Val Thr Leu Phe Cys 195 200 205 ggc gca ggc gta cgc gac gcc cgc gag cag gtc ctc gcc ctg gcg gag 972 Gly Ala Gly Val Arg Asp Ala Arg Glu Gln Val Leu Ala Leu Ala Glu 210 215 220 aag atc aag gcc ccc atc ggc cac gcc ctc ggc ggc aag atg cac atc 1020 Lys Ile Lys Ala Pro Ile Gly His Ala Leu Gly Gly Lys Met His Ile 225 230 235 240 cag cac gac aac ccc ttc gac gtc ggc atg tcc ggg ctc ctc ggc tac 1068 Gln His Asp Asn Pro Phe Asp Val Gly Met Ser Gly Leu Leu Gly Tyr 245 250 255 ggc gcc tgc cac gac gca ctg cac gac gcc gac ctg ctc atc ctc ctg 1116 Gly Ala Cys His Asp Ala Leu His Asp Ala Asp Leu Leu Ile Leu Leu 260 265 270 ggc acc gac ttc ccc tac acc gag ttc ctg ccc acc ggg aac gtc gcg 1164 Gly Thr Asp Phe Pro Tyr Thr Glu Phe Leu Pro Thr Gly Asn Val Ala 275 280 285 cag gtc gac agc gac gcc gcg cac atc ggc cgc cgc acc acg gtg cac 1212 Gln Val Asp Ser Asp Ala Ala His Ile Gly Arg Arg Thr Thr Val His 290 295 300 cac ccg gtg gtc ggc gac gtc ggc gcc acc ctc gac acc atc ctc ccg 1260 His Pro Val Val Gly Asp Val Gly Ala Thr Leu Asp Thr Ile Leu Pro 305 310 315 320 cac ctg gag gag aag acc gac cgc agc ttc ctc gac ggg atg ctc cgc 1308 His Leu Glu Glu Lys Thr Asp Arg Ser Phe Leu Asp Gly Met Leu Arg 325 330 335 aag cac gcc cgg acg ctg gag aag gtc gtg gag gtg tac acg cgg gac 1356 Lys His Ala Arg Thr Leu Glu Lys Val Val Glu Val Tyr Thr Arg Asp 340 345 350 gtc ggg aag cac acc ccc atc cac ccg gag tac ctc gcc gcc gtc ctc 1404 Val Gly Lys His Thr Pro Ile His Pro Glu Tyr Leu Ala Ala Val Leu 355 360 365 gac gag ctc gcc gcc gac gac gcc gtg ttc acc gtc gac acc ggc atg 1452 Asp Glu Leu Ala Ala Asp Asp Ala Val Phe Thr Val Asp Thr Gly Met 370 375 380 tgc aac gtg tgg gcc gcg cgc tac ctc acc ccc aac gga cga cgc gcg 1500 Cys Asn Val Trp Ala Ala Arg Tyr Leu Thr Pro Asn Gly Arg Arg Ala 385 390 395 400 cag atc ggc tcc ctc cgc cac ggc acg atg gcc aac gcg ctg ccg cac 1548 Gln Ile Gly Ser Leu Arg His Gly Thr Met Ala Asn Ala Leu Pro His 405 410 415 gcc atc ggc gcg cag ttc gcc gac cgg aac cgt cag gtc atc agc ctc 1596 Ala Ile Gly Ala Gln Phe Ala Asp Arg Asn Arg Gln Val Ile Ser Leu 420 425 430 aac ggc gac ggc ggc ctc gcc atg ctc ctc ggc gaa ctg ctc acc gtg 1644 Asn Gly Asp Gly Gly Leu Ala Met Leu Leu Gly Glu Leu Leu Thr Val 435 440 445 gaa ctc cac gac ctg ccc gtg aag atg gtc gtg ttc aac aac tcc tcg 1692 Glu Leu His Asp Leu Pro Val Lys Met Val Val Phe Asn Asn Ser Ser 450 455 460 ctc ggc atg gtc aaa ctc gag atg ctc gtc gag ggc atg ccc cag cac 1740 Leu Gly Met Val Lys Leu Glu Met Leu Val Glu Gly Met Pro Gln His 465 470 475 480 ggc acc gac cac ggg cag gtc gac ttc gcg gcg atc gcg gcc gcc gcg 1788 Gly Thr Asp His Gly Gln Val Asp Phe Ala Ala Ile Ala Ala Ala Ala 485 490 495 ggc atc cac tcc gtg ctc atc gac gac ccc gcc acc ctc cgc gag cag 1836 Gly Ile His Ser Val Leu Ile Asp Asp Pro Ala Thr Leu Arg Glu Gln 500 505 510 ctc gcc gcg gcg ctc gcc cac ccc ggg ccc gtg ctc atc gac gtc cgc 1884 Leu Ala Ala Ala Leu Ala His Pro Gly Pro Val Leu Ile Asp Val Arg 515 520 525 acc gac ccc gac gcc ctc tcc ctc ccg ccg gag atc acc tgg gag atg 1932 Thr Asp Pro Asp Ala Leu Ser Leu Pro Pro Glu Ile Thr Trp Glu Met 530 535 540 ctc atc ggc ttc tcc cgg gcc gcg acc cgt acc gtc ctc ggc ggc ggc 1980 Leu Ile Gly Phe Ser Arg Ala Ala Thr Arg Thr Val Leu Gly Gly Gly 545 550 555 560 gtc ggc gag atg gtg cac ctc gcg cgc acg aac ctg cgc aac atc ggc 2028 Val Gly Glu Met Val His Leu Ala Arg Thr Asn Leu Arg Asn Ile Gly 565 570 575 gcg gcc ggg gcg gtc tag tctgggcgtc atgaccccga ccgtccggat 2076 Ala Ala Gly Ala Val 580 cgacctcgcc cgcctcgacg cgaacatcgc cgcgcaaccc gggcggctgc gaccgcacgt 2136 gaagacccac aagatcctgg agatcgcccg ccggcagctc gcggcgggtg cccacggcct 2196 cacggtcgcg acggtggggg aggcggaggt cttcgccgag gtctgcgacg acatcttcat 2256 cgcctacccc gtctgggccg acaagaggct gcgcgacctg gccgggaggg tgcggctgag 2316 cgtcggctgc gactcgctgg ccgccgccga 2346 <210> 140 <211> 581 <212> PRT <213> Corynebacterium humireducens <400> 140 Met Ala Arg Thr Tyr Ala Gln Gln Leu Val Asp Ala Leu Glu Arg Gln 1 5 10 15 Gly Val Glu Arg Ile Tyr Gly Val Val Gly Asp Ser Leu Asn Pro Ile 20 25 30 Val Asp Ala Val Arg Thr Ser Pro Ile Glu Trp Ile His Val Arg Asn 35 40 45 Glu Glu Ala Ala Ala Phe Ala Ala Gly Ala Glu Ser Leu Leu Thr Gly 50 55 60 Arg Leu Ala Val Cys Ala Gly Ser Cys Gly Pro Gly Asn Leu His Leu 65 70 75 80 Ile Gln Gly Leu Tyr Asp Ala His Arg Asn Gly Ala His Val Leu Ala 85 90 95 Leu Ala Ser His Ile Pro Ser Asn Gln Ile Gly Thr Ser Tyr Phe Gln 100 105 110 Glu Thr His Pro Glu Gln Leu Phe Ala Glu Cys Ser Gly Tyr Cys Glu 115 120 125 Met Val Asn Ser Ala Asp Gln Gly Ala Arg Val Leu His His Ala Ile 130 135 140 Gln Ser Thr Met Ala Gly Gln Gly Val Ser Val Leu Val Ile Pro Gly 145 150 155 160 Asp Ile Ala Thr Gln Asp Ala Gly Asp Asp Arg Phe Leu Gln Ser Thr 165 170 175 Val Ala Thr Gly Arg Pro Val Leu His Pro Asp Ala Arg Glu Ala Ala 180 185 190 Ala Leu Val Ala Ala Ile Asn Ala Ala Asp Thr Val Thr Leu Phe Cys 195 200 205 Gly Ala Gly Val Arg Asp Ala Arg Glu Gln Val Leu Ala Leu Ala Glu 210 215 220 Lys Ile Lys Ala Pro Ile Gly His Ala Leu Gly Gly Lys Met His Ile 225 230 235 240 Gln His Asp Asn Pro Phe Asp Val Gly Met Ser Gly Leu Leu Gly Tyr 245 250 255 Gly Ala Cys His Asp Ala Leu His Asp Ala Asp Leu Leu Ile Leu Leu 260 265 270 Gly Thr Asp Phe Pro Tyr Thr Glu Phe Leu Pro Thr Gly Asn Val Ala 275 280 285 Gln Val Asp Ser Asp Ala Ala His Ile Gly Arg Arg Thr Thr Val His 290 295 300 His Pro Val Val Gly Asp Val Gly Ala Thr Leu Asp Thr Ile Leu Pro 305 310 315 320 His Leu Glu Glu Lys Thr Asp Arg Ser Phe Leu Asp Gly Met Leu Arg 325 330 335 Lys His Ala Arg Thr Leu Glu Lys Val Val Glu Val Tyr Thr Arg Asp 340 345 350 Val Gly Lys His Thr Pro Ile His Pro Glu Tyr Leu Ala Ala Val Leu 355 360 365 Asp Glu Leu Ala Ala Asp Asp Ala Val Phe Thr Val Asp Thr Gly Met 370 375 380 Cys Asn Val Trp Ala Ala Arg Tyr Leu Thr Pro Asn Gly Arg Arg Ala 385 390 395 400 Gln Ile Gly Ser Leu Arg His Gly Thr Met Ala Asn Ala Leu Pro His 405 410 415 Ala Ile Gly Ala Gln Phe Ala Asp Arg Asn Arg Gln Val Ile Ser Leu 420 425 430 Asn Gly Asp Gly Gly Leu Ala Met Leu Leu Gly Glu Leu Leu Thr Val 435 440 445 Glu Leu His Asp Leu Pro Val Lys Met Val Val Phe Asn Asn Ser Ser 450 455 460 Leu Gly Met Val Lys Leu Glu Met Leu Val Glu Gly Met Pro Gln His 465 470 475 480 Gly Thr Asp His Gly Gln Val Asp Phe Ala Ala Ile Ala Ala Ala Ala 485 490 495 Gly Ile His Ser Val Leu Ile Asp Asp Pro Ala Thr Leu Arg Glu Gln 500 505 510 Leu Ala Ala Ala Leu Ala His Pro Gly Pro Val Leu Ile Asp Val Arg 515 520 525 Thr Asp Pro Asp Ala Leu Ser Leu Pro Pro Glu Ile Thr Trp Glu Met 530 535 540 Leu Ile Gly Phe Ser Arg Ala Ala Thr Arg Thr Val Leu Gly Gly Gly 545 550 555 560 Val Gly Glu Met Val His Leu Ala Arg Thr Asn Leu Arg Asn Ile Gly 565 570 575 Ala Ala Gly Ala Val 580 <210> 141 <211> 2010 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1710) <223> gene pyruvate kinase_1 <400> 141 acgagctgct gtggaacctc ctcatcttcg ggctgctcat cctcatcgac cgccgcttcc 60 gcatcgcacc gggtggactg ttcgccctct acgtcgccgg ttacacgctg ggccgtttct 120 ggatcgagct catgcgcgac gattccgcca cgatgatcct cggccagcgg gtcaacacct 180 gggtctccgc cgtggtgttc ctcgtctcga tcgcggtatt cgtgtggttg cagcgacgtc 240 agcgggctgt tgtccgagaa acagagtagg ttggtagccg tgtctagacg aaccaagatt 300 gtg tgc acc ctc ggt cct gcc gtc gcc tcc aag gac gcg atc acg cgt 348 Val Cys Thr Leu Gly Pro Ala Val Ala Ser Lys Asp Ala Ile Thr Arg 1 5 10 15 ctg gtg gag gac ggc atg gac gtc gcc cgc ctg aac ttc tcc cac ggt 396 Leu Val Glu Asp Gly Met Asp Val Ala Arg Leu Asn Phe Ser His Gly 20 25 30 gat cac ccc gat cac gag cag aac tac aag tgg gtc cgg gaa gcc acc 444 Asp His Pro Asp His Glu Gln Asn Tyr Lys Trp Val Arg Glu Ala Thr 35 40 45 gat gag acc ggc cgt gcc gtc ggc atc ctc gct gac ctc cag ggc ccg 492 Asp Glu Thr Gly Arg Ala Val Gly Ile Leu Ala Asp Leu Gln Gly Pro 50 55 60 aag atc cgc ctc ggt cgc ttc atc gac ggt gcc acc gtc tgg gag acc 540 Lys Ile Arg Leu Gly Arg Phe Ile Asp Gly Ala Thr Val Trp Glu Thr 65 70 75 80 ggt gag gtc atc cgc atc acc gtg gac gat gtc gag ggc acg cac gac 588 Gly Glu Val Ile Arg Ile Thr Val Asp Asp Val Glu Gly Thr His Asp 85 90 95 cgc gtg tcc acc acc tac aag aac ctc gcg cag gac gcc aag ccc ggc 636 Arg Val Ser Thr Thr Tyr Lys Asn Leu Ala Gln Asp Ala Lys Pro Gly 100 105 110 gac cgt ctg ctc gtc gac gac ggc aag gtc ggc ctg acc tgc ctc gag 684 Asp Arg Leu Leu Val Asp Asp Gly Lys Val Gly Leu Thr Cys Leu Glu 115 120 125 gtc gac ggc aac gac gtc gtc tgc gag gtc acc gag ggc ggc ccc gtc 732 Val Asp Gly Asn Asp Val Val Cys Glu Val Thr Glu Gly Gly Pro Val 130 135 140 tcc aac aac aag ggc gtc tcc ctc ccg ggc atg gac atc tct gtg ccg 780 Ser Asn Asn Lys Gly Val Ser Leu Pro Gly Met Asp Ile Ser Val Pro 145 150 155 160 gcg ctg tcg gag aag gac atc gcc gac ctc gag ttc gcc ctc aag ctg 828 Ala Leu Ser Glu Lys Asp Ile Ala Asp Leu Glu Phe Ala Leu Lys Leu 165 170 175 ggc gtc gac ttc atc gcc ctg tcc ttc gtc cgc tcc ccg gcg gac gtc 876 Gly Val Asp Phe Ile Ala Leu Ser Phe Val Arg Ser Pro Ala Asp Val 180 185 190 gac ctc gtc cac gag gtc atg gac cgc gtc ggc cgc cac gtg ccg gtc 924 Asp Leu Val His Glu Val Met Asp Arg Val Gly Arg His Val Pro Val 195 200 205 atc gcg aag ctg gag aag ccg gag gcc gtc cac gcc ctc gag tcc atc 972 Ile Ala Lys Leu Glu Lys Pro Glu Ala Val His Ala Leu Glu Ser Ile 210 215 220 gtc ctc gcc ttc gac gcc atc atg gtc gcc cgc ggt gac ctc ggc gtc 1020 Val Leu Ala Phe Asp Ala Ile Met Val Ala Arg Gly Asp Leu Gly Val 225 230 235 240 gag gtg ccg ctg gag cag gtc ccg ctg gtg cag aag cgg gcc atc cag 1068 Glu Val Pro Leu Glu Gln Val Pro Leu Val Gln Lys Arg Ala Ile Gln 245 250 255 atc gcc cgc gag aac gcg aag ccg gtc att gtc gcc acg cag atg ctc 1116 Ile Ala Arg Glu Asn Ala Lys Pro Val Ile Val Ala Thr Gln Met Leu 260 265 270 gac tcc atg atc gag aac tcc acc ccg acc cgc gcc gag gcc tcc gac 1164 Asp Ser Met Ile Glu Asn Ser Thr Pro Thr Arg Ala Glu Ala Ser Asp 275 280 285 gtc gcc aac gcc gtc ctc gac ggc gcc gac gcg gtc atg ctc tcc ggt 1212 Val Ala Asn Ala Val Leu Asp Gly Ala Asp Ala Val Met Leu Ser Gly 290 295 300 gag acc tcc atc ggt gtg gac ccg cac aac gtc gtg cgc acc atg gcc 1260 Glu Thr Ser Ile Gly Val Asp Pro His Asn Val Val Arg Thr Met Ala 305 310 315 320 cgc atc gtc acc cag gcc gag acc gac ggc cac gtg ccg ccg ctg agc 1308 Arg Ile Val Thr Gln Ala Glu Thr Asp Gly His Val Pro Pro Leu Ser 325 330 335 cat gtc ccg cgc acc aag cgg ggc gtc atc tcc tac tcg gcc cgt gac 1356 His Val Pro Arg Thr Lys Arg Gly Val Ile Ser Tyr Ser Ala Arg Asp 340 345 350 atc gcc gag cgt ctc aac gcc cgc gcc ctg gtc tgc ttc acc acc tcc 1404 Ile Ala Glu Arg Leu Asn Ala Arg Ala Leu Val Cys Phe Thr Thr Ser 355 360 365 ggt gac acc gcc cgc cgt gtc gcc cgc ctg cac tcg cac ctg ccg ctg 1452 Gly Asp Thr Ala Arg Arg Val Ala Arg Leu His Ser His Leu Pro Leu 370 375 380 ctg gtg ttc acc ccg cac ccg gcg gtc cgt tcg cag ctg gcc ctc acc 1500 Leu Val Phe Thr Pro His Pro Ala Val Arg Ser Gln Leu Ala Leu Thr 385 390 395 400 tgg ggc gcc gag acc ttc ctc tgc gcc cag gcc ctg tcc acc gac gac 1548 Trp Gly Ala Glu Thr Phe Leu Cys Ala Gln Ala Leu Ser Thr Asp Asp 405 410 415 atg atg gcc gag ctg gac cgt tcc ctg ctc gcc atg gac gac tac cac 1596 Met Met Ala Glu Leu Asp Arg Ser Leu Leu Ala Met Asp Asp Tyr His 420 425 430 cgc gac gac atg atg gtc gtc gtc gcc ggc acc ccg ccc ggg gtc tcc 1644 Arg Asp Asp Met Met Val Val Val Ala Gly Thr Pro Pro Gly Val Ser 435 440 445 ggc aac acg aac atg atc cac gtc cac ctg ctg ggc gag gac acg agt 1692 Gly Asn Thr Asn Met Ile His Val His Leu Leu Gly Glu Asp Thr Ser 450 455 460 tcc ccg acc ggc agg tag ccggagaaca gaaaaaggag cggcggagag 1740 Ser Pro Thr Gly Arg 465 gatcatcctc tccgccgctc ctgtcgtgtg cggctacttc accggggtcg gctccagctt 1800 ccacacctca cgcgcgtagt cgttgatggt gcggtcggag gagaaacggc ccgaggcgca 1860 gatgttcgcc cagcacatac gcgcccagtg caggcggtcg gcgtagtcgg cggccatgcg 1920 gtcgcgggtc tcgcggtagt cggcgaagtc accgaggacg tagtagacgt ccggggtctc 1980 ccagccgccg ccgtcgagaa gcgaggaacg 2010 <210> 142 <211> 469 <212> PRT <213> Corynebacterium humireducens <400> 142 Val Cys Thr Leu Gly Pro Ala Val Ala Ser Lys Asp Ala Ile Thr Arg 1 5 10 15 Leu Val Glu Asp Gly Met Asp Val Ala Arg Leu Asn Phe Ser His Gly 20 25 30 Asp His Pro Asp His Glu Gln Asn Tyr Lys Trp Val Arg Glu Ala Thr 35 40 45 Asp Glu Thr Gly Arg Ala Val Gly Ile Leu Ala Asp Leu Gln Gly Pro 50 55 60 Lys Ile Arg Leu Gly Arg Phe Ile Asp Gly Ala Thr Val Trp Glu Thr 65 70 75 80 Gly Glu Val Ile Arg Ile Thr Val Asp Asp Val Glu Gly Thr His Asp 85 90 95 Arg Val Ser Thr Thr Tyr Lys Asn Leu Ala Gln Asp Ala Lys Pro Gly 100 105 110 Asp Arg Leu Leu Val Asp Asp Gly Lys Val Gly Leu Thr Cys Leu Glu 115 120 125 Val Asp Gly Asn Asp Val Val Cys Glu Val Thr Glu Gly Gly Pro Val 130 135 140 Ser Asn Asn Lys Gly Val Ser Leu Pro Gly Met Asp Ile Ser Val Pro 145 150 155 160 Ala Leu Ser Glu Lys Asp Ile Ala Asp Leu Glu Phe Ala Leu Lys Leu 165 170 175 Gly Val Asp Phe Ile Ala Leu Ser Phe Val Arg Ser Pro Ala Asp Val 180 185 190 Asp Leu Val His Glu Val Met Asp Arg Val Gly Arg His Val Pro Val 195 200 205 Ile Ala Lys Leu Glu Lys Pro Glu Ala Val His Ala Leu Glu Ser Ile 210 215 220 Val Leu Ala Phe Asp Ala Ile Met Val Ala Arg Gly Asp Leu Gly Val 225 230 235 240 Glu Val Pro Leu Glu Gln Val Pro Leu Val Gln Lys Arg Ala Ile Gln 245 250 255 Ile Ala Arg Glu Asn Ala Lys Pro Val Ile Val Ala Thr Gln Met Leu 260 265 270 Asp Ser Met Ile Glu Asn Ser Thr Pro Thr Arg Ala Glu Ala Ser Asp 275 280 285 Val Ala Asn Ala Val Leu Asp Gly Ala Asp Ala Val Met Leu Ser Gly 290 295 300 Glu Thr Ser Ile Gly Val Asp Pro His Asn Val Val Arg Thr Met Ala 305 310 315 320 Arg Ile Val Thr Gln Ala Glu Thr Asp Gly His Val Pro Pro Leu Ser 325 330 335 His Val Pro Arg Thr Lys Arg Gly Val Ile Ser Tyr Ser Ala Arg Asp 340 345 350 Ile Ala Glu Arg Leu Asn Ala Arg Ala Leu Val Cys Phe Thr Thr Ser 355 360 365 Gly Asp Thr Ala Arg Arg Val Ala Arg Leu His Ser His Leu Pro Leu 370 375 380 Leu Val Phe Thr Pro His Pro Ala Val Arg Ser Gln Leu Ala Leu Thr 385 390 395 400 Trp Gly Ala Glu Thr Phe Leu Cys Ala Gln Ala Leu Ser Thr Asp Asp 405 410 415 Met Met Ala Glu Leu Asp Arg Ser Leu Leu Ala Met Asp Asp Tyr His 420 425 430 Arg Asp Asp Met Met Val Val Val Ala Gly Thr Pro Pro Gly Val Ser 435 440 445 Gly Asn Thr Asn Met Ile His Val His Leu Leu Gly Glu Asp Thr Ser 450 455 460 Ser Pro Thr Gly Arg 465 <210> 143 <211> 2472 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(2172) <223> gene pyruvate kinase 2 <400> 143 atccagaacc aggacgtcgc tctgccggtg tccgcgtacc tcgacggcga gtacgggcag 60 aaggacctct acatcggcac cccggcgatc atcaaccgcg ccggaatcgc ccgcacggtc 120 gagctgaagc tcaacgagca cgaggcggag cgcttcgccc actcggctga cacgctgcgc 180 gcgatcaagg aggagttctt ccccgccgac tgacggcttc cccgagatgc cgaacctccc 240 gaacacaggc acaatatggg ggtatcaccc attttgtcgt gcgcgtacgg gaggacagcc 300 gtg gat gcc agg ctg cag gaa ctg atc acc gag gtc gac ggt ctc atc 348 Val Asp Ala Arg Leu Gln Glu Leu Ile Thr Glu Val Asp Gly Leu Ile 1 5 10 15 gac tgc ctc aag gag gtc gag gag cag gtg gcc ccc gcg atc ggg cgg 396 Asp Cys Leu Lys Glu Val Glu Glu Gln Val Ala Pro Ala Ile Gly Arg 20 25 30 gtc gcc gag aac cac cgc cac ggc gcg gtg aac ctc gtc cac tac gcc 444 Val Ala Glu Asn His Arg His Gly Ala Val Asn Leu Val His Tyr Ala 35 40 45 gag ctg cgg cag cac gac gtc cgc gcg gtc cag ggc ggc ctg gcc tcc 492 Glu Leu Arg Gln His Asp Val Arg Ala Val Gln Gly Gly Leu Ala Ser 50 55 60 atc ggc gcc acc cgc ctg tcg acg gcg gag ccg gcc gtc ctc gcc cgt 540 Ile Gly Ala Thr Arg Leu Ser Thr Ala Glu Pro Ala Val Leu Ala Arg 65 70 75 80 ctc cac gcg gcg cgg aac gtg ctc tcc gcc tac aac ggt gag cag ctc 588 Leu His Ala Ala Arg Asn Val Leu Ser Ala Tyr Asn Gly Glu Gln Leu 85 90 95 aag tac acc ggc agc gag gtc cgt gac gcc ttc gcc acc gcc gac gac 636 Lys Tyr Thr Gly Ser Glu Val Arg Asp Ala Phe Ala Thr Ala Asp Asp 100 105 110 atc ctc gag gac cac gcc ctg cag ctc ctc ggc tac tcc tcc gag gag 684 Ile Leu Glu Asp His Ala Leu Gln Leu Leu Gly Tyr Ser Ser Glu Glu 115 120 125 acg cac tcc cgc atc atg gtg acc ctg ccg acg gag gca ggc gag gac 732 Thr His Ser Arg Ile Met Val Thr Leu Pro Thr Glu Ala Gly Glu Asp 130 135 140 ctc gac ctg gtc cgc ggt ttc gtc gag gcc ggc atg gag ctg gcc cgc 780 Leu Asp Leu Val Arg Gly Phe Val Glu Ala Gly Met Glu Leu Ala Arg 145 150 155 160 atc aac tgc gcc cac gac ggc ccc gag gtg tgg agg cgc atg atc gac 828 Ile Asn Cys Ala His Asp Gly Pro Glu Val Trp Arg Arg Met Ile Asp 165 170 175 aac gtc cac acc gcc gcc cgc gag gcc ggc cgg gag atc agg atc gcc 876 Asn Val His Thr Ala Ala Arg Glu Ala Gly Arg Glu Ile Arg Ile Ala 180 185 190 atg gac ctg gcg ggc ccg aag gtc cgc acc ggg gag atc gag ccg ggc 924 Met Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu Ile Glu Pro Gly 195 200 205 ccg gag gtc ggc cgc gcc cgc gtc acc cgc gag gac acc ggc gtg gtg 972 Pro Glu Val Gly Arg Ala Arg Val Thr Arg Glu Asp Thr Gly Val Val 210 215 220 gtc acc ccg tcg aag atc tgg ctc acc ccc gcc gac ctg gcc gac gac 1020 Val Thr Pro Ser Lys Ile Trp Leu Thr Pro Ala Asp Leu Ala Asp Asp 225 230 235 240 gag gtc ccg ccg gtc ccc gcc gga ctg ccg ggc cgc ccg acg ctg ccg 1068 Glu Val Pro Pro Val Pro Ala Gly Leu Pro Gly Arg Pro Thr Leu Pro 245 250 255 ctg cgc gtc gag cgt gac tgg ctg gag gcc ctg gag atc ggc tcc gag 1116 Leu Arg Val Glu Arg Asp Trp Leu Glu Ala Leu Glu Ile Gly Ser Glu 260 265 270 atc acc ttc cac gac aac cgc gac gcg aag cgc cgg ttc acc gtc acc 1164 Ile Thr Phe His Asp Asn Arg Asp Ala Lys Arg Arg Phe Thr Val Thr 275 280 285 cgc atc gag ggg cag ggg gtg ctg ctg gag ggg cgt cgc aac gcc tac 1212 Arg Ile Glu Gly Gln Gly Val Leu Leu Glu Gly Arg Arg Asn Ala Tyr 290 295 300 gtc tcc aac tcc acc ctc ctc gag acc gac tgg acc aag acc cgc gtc 1260 Val Ser Asn Ser Thr Leu Leu Glu Thr Asp Trp Thr Lys Thr Arg Val 305 310 315 320 tcc ggc gtc ccg ccg acc gag cag cgc ctg cgt ctg agc atc gga gac 1308 Ser Gly Val Pro Pro Thr Glu Gln Arg Leu Arg Leu Ser Ile Gly Asp 325 330 335 acg ctc atc ctc acc gac gac cag acc ccc gcc gtc gtc gcc ccg gac 1356 Thr Leu Ile Leu Thr Asp Asp Gln Thr Pro Ala Val Val Ala Pro Asp 340 345 350 gtc acc ccg cgc atc ggc tgc acg ctg ccg gag gcc gtc gcc gcc atc 1404 Val Thr Pro Arg Ile Gly Cys Thr Leu Pro Glu Ala Val Ala Ala Ile 355 360 365 gag gtc ggg cag aag gtg ctt ttc gac gac ggc tcg atc gcc gcc cgc 1452 Glu Val Gly Gln Lys Val Leu Phe Asp Asp Gly Ser Ile Ala Ala Arg 370 375 380 gcc gtc gac aag cag gtc acg gag cag ggc cac cac gag gtg cgc ctc 1500 Ala Val Asp Lys Gln Val Thr Glu Gln Gly His His Glu Val Arg Leu 385 390 395 400 gag gtg acc tac gcc ggg ccg gag ggc acc aag ctc gcc gcg tac aag 1548 Glu Val Thr Tyr Ala Gly Pro Glu Gly Thr Lys Leu Ala Ala Tyr Lys 405 410 415 ggc atc aac ctg ccg gac acc gtc ctg ccg ctg ccg agc ctc acc gag 1596 Gly Ile Asn Leu Pro Asp Thr Val Leu Pro Leu Pro Ser Leu Thr Glu 420 425 430 gag gac atc gag gcg ttc cgc ttc gtg gcc gag cac ggt gac atc gcg 1644 Glu Asp Ile Glu Ala Phe Arg Phe Val Ala Glu His Gly Asp Ile Ala 435 440 445 aac gtc tcc ttc att cgc gac gcc gac gac gtc aac ttc gtg ctc gac 1692 Asn Val Ser Phe Ile Arg Asp Ala Asp Asp Val Asn Phe Val Leu Asp 450 455 460 acc ctc gag cag atc gcg cag gag tcc gac gac ccg gag cgc gtc cgc 1740 Thr Leu Glu Gln Ile Ala Gln Glu Ser Asp Asp Pro Glu Arg Val Arg 465 470 475 480 aac ctc ggc atc gtg ctc aag atc gag acg atc ccc gcc tac gag ggc 1788 Asn Leu Gly Ile Val Leu Lys Ile Glu Thr Ile Pro Ala Tyr Glu Gly 485 490 495 ctg tgc agc gtc ctg ctc gcc ggc atg cgc cac ccc aac ctc gga gtc 1836 Leu Cys Ser Val Leu Leu Ala Gly Met Arg His Pro Asn Leu Gly Val 500 505 510 atg atc gcc cgc ggc gac ctg gcc gtc gag ctc ggc ttc gag cgc atg 1884 Met Ile Ala Arg Gly Asp Leu Ala Val Glu Leu Gly Phe Glu Arg Met 515 520 525 gcc gag gtc ccg cgc ctc atc gcc cag atg gcg gag gcc gcc cac gtg 1932 Ala Glu Val Pro Arg Leu Ile Ala Gln Met Ala Glu Ala Ala His Val 530 535 540 ccc gtc atc atg gcc acc cag gtg ctc gag aac ctg gcc aag tcc ggc 1980 Pro Val Ile Met Ala Thr Gln Val Leu Glu Asn Leu Ala Lys Ser Gly 545 550 555 560 ctg ccg acc cgc gcc gag atc acc gac gcc gcg tac gcc ctg cgc tcc 2028 Leu Pro Thr Arg Ala Glu Ile Thr Asp Ala Ala Tyr Ala Leu Arg Ser 565 570 575 gag gcc gtg atg ctc aac aag ggc ccg cac atc acc gac gcg atc cgc 2076 Glu Ala Val Met Leu Asn Lys Gly Pro His Ile Thr Asp Ala Ile Arg 580 585 590 atc ctc cac acc ctg tcg acc aag ctc ggc cgt tcc cag cgc aag aac 2124 Ile Leu His Thr Leu Ser Thr Lys Leu Gly Arg Ser Gln Arg Lys Asn 595 600 605 cgc cag atg ctc cgc cag atc tcg agc tgg atg ccc ggg aat gag tag 2172 Arg Gln Met Leu Arg Gln Ile Ser Ser Trp Met Pro Gly Asn Glu 610 615 620 atgaccgccg cacccaggat catcgcccac cgcggttact ccgcgaagta ccccgagttg 2232 acgcccgtcg ccttcgagcg ggcgctggaa ctgcccgtcc acggcatcga gtgcgatgta 2292 cgcctcagcc tcgacgggga ggtggtggtg ttgcatgacc cgctcatcga ccgcgtcgcg 2352 gacggctccg ggcgggtctc ccgcatgacg gtggacgccc tgcgtgacca caactacggc 2412 accgccgagc tgccgcagga catgctcacc ctcccggagc tgctggagat ggtggagccg 2472 <210> 144 <211> 623 <212> PRT <213> Corynebacterium humireducens <400> 144 Val Asp Ala Arg Leu Gln Glu Leu Ile Thr Glu Val Asp Gly Leu Ile 1 5 10 15 Asp Cys Leu Lys Glu Val Glu Glu Gln Val Ala Pro Ala Ile Gly Arg 20 25 30 Val Ala Glu Asn His Arg His Gly Ala Val Asn Leu Val His Tyr Ala 35 40 45 Glu Leu Arg Gln His Asp Val Arg Ala Val Gln Gly Gly Leu Ala Ser 50 55 60 Ile Gly Ala Thr Arg Leu Ser Thr Ala Glu Pro Ala Val Leu Ala Arg 65 70 75 80 Leu His Ala Ala Arg Asn Val Leu Ser Ala Tyr Asn Gly Glu Gln Leu 85 90 95 Lys Tyr Thr Gly Ser Glu Val Arg Asp Ala Phe Ala Thr Ala Asp Asp 100 105 110 Ile Leu Glu Asp His Ala Leu Gln Leu Leu Gly Tyr Ser Ser Glu Glu 115 120 125 Thr His Ser Arg Ile Met Val Thr Leu Pro Thr Glu Ala Gly Glu Asp 130 135 140 Leu Asp Leu Val Arg Gly Phe Val Glu Ala Gly Met Glu Leu Ala Arg 145 150 155 160 Ile Asn Cys Ala His Asp Gly Pro Glu Val Trp Arg Arg Met Ile Asp 165 170 175 Asn Val His Thr Ala Ala Arg Glu Ala Gly Arg Glu Ile Arg Ile Ala 180 185 190 Met Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu Ile Glu Pro Gly 195 200 205 Pro Glu Val Gly Arg Ala Arg Val Thr Arg Glu Asp Thr Gly Val Val 210 215 220 Val Thr Pro Ser Lys Ile Trp Leu Thr Pro Ala Asp Leu Ala Asp Asp 225 230 235 240 Glu Val Pro Pro Val Pro Ala Gly Leu Pro Gly Arg Pro Thr Leu Pro 245 250 255 Leu Arg Val Glu Arg Asp Trp Leu Glu Ala Leu Glu Ile Gly Ser Glu 260 265 270 Ile Thr Phe His Asp Asn Arg Asp Ala Lys Arg Arg Phe Thr Val Thr 275 280 285 Arg Ile Glu Gly Gln Gly Val Leu Leu Glu Gly Arg Arg Asn Ala Tyr 290 295 300 Val Ser Asn Ser Thr Leu Leu Glu Thr Asp Trp Thr Lys Thr Arg Val 305 310 315 320 Ser Gly Val Pro Pro Thr Glu Gln Arg Leu Arg Leu Ser Ile Gly Asp 325 330 335 Thr Leu Ile Leu Thr Asp Asp Gln Thr Pro Ala Val Val Ala Pro Asp 340 345 350 Val Thr Pro Arg Ile Gly Cys Thr Leu Pro Glu Ala Val Ala Ala Ile 355 360 365 Glu Val Gly Gln Lys Val Leu Phe Asp Asp Gly Ser Ile Ala Ala Arg 370 375 380 Ala Val Asp Lys Gln Val Thr Glu Gln Gly His His Glu Val Arg Leu 385 390 395 400 Glu Val Thr Tyr Ala Gly Pro Glu Gly Thr Lys Leu Ala Ala Tyr Lys 405 410 415 Gly Ile Asn Leu Pro Asp Thr Val Leu Pro Leu Pro Ser Leu Thr Glu 420 425 430 Glu Asp Ile Glu Ala Phe Arg Phe Val Ala Glu His Gly Asp Ile Ala 435 440 445 Asn Val Ser Phe Ile Arg Asp Ala Asp Asp Val Asn Phe Val Leu Asp 450 455 460 Thr Leu Glu Gln Ile Ala Gln Glu Ser Asp Asp Pro Glu Arg Val Arg 465 470 475 480 Asn Leu Gly Ile Val Leu Lys Ile Glu Thr Ile Pro Ala Tyr Glu Gly 485 490 495 Leu Cys Ser Val Leu Leu Ala Gly Met Arg His Pro Asn Leu Gly Val 500 505 510 Met Ile Ala Arg Gly Asp Leu Ala Val Glu Leu Gly Phe Glu Arg Met 515 520 525 Ala Glu Val Pro Arg Leu Ile Ala Gln Met Ala Glu Ala Ala His Val 530 535 540 Pro Val Ile Met Ala Thr Gln Val Leu Glu Asn Leu Ala Lys Ser Gly 545 550 555 560 Leu Pro Thr Arg Ala Glu Ile Thr Asp Ala Ala Tyr Ala Leu Arg Ser 565 570 575 Glu Ala Val Met Leu Asn Lys Gly Pro His Ile Thr Asp Ala Ile Arg 580 585 590 Ile Leu His Thr Leu Ser Thr Lys Leu Gly Arg Ser Gln Arg Lys Asn 595 600 605 Arg Gln Met Leu Arg Gln Ile Ser Ser Trp Met Pro Gly Asn Glu 610 615 620 <210> 145 <211> 1878 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1578) <223> gene enolase <400> 145 ccctgggggc ggcgcacctg ctgtgtacca acggtgggga cctcagcacc ccggagggct 60 ggtcccgggc gatctacgcc tacaaccgtt ccgaggccta cctcatcgac gtgcgtgacg 120 cggccgcgtc ctatgccctg aaccagccgg cccggtgacg gccacccctg gttgccccct 180 gcaatcgtcg ggttgtagcc cctggtggcg gtttttctgg aagaattgtc cccggacgcg 240 tgtcgagagc gcgcgacgat gacgaactcc catctcatat ggctatagga ggccccaaaa 300 gtg gct gac atc att cac gtt ttc gca cga gag atc atg gat tcc cgt 348 Val Ala Asp Ile Ile His Val Phe Ala Arg Glu Ile Met Asp Ser Arg 1 5 10 15 ggc aac ccg acc gtc gag gcc ggc gtt gtg ctg gat gac gga acc ctc 396 Gly Asn Pro Thr Val Glu Ala Gly Val Val Leu Asp Asp Gly Thr Leu 20 25 30 gcc acc gcc ggt gtc ccc tcc ggc gcc tcc acc ggt gtc cac gag gca 444 Ala Thr Ala Gly Val Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala 35 40 45 cac gag ctg cgt gac ggc ggc gac cgc tac ctg ggc aag ggt gtc ctg 492 His Glu Leu Arg Asp Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu 50 55 60 aag gcc gtc gag aac gtc aac gag gag atc gcc gac gag ctg gcc ggc 540 Lys Ala Val Glu Asn Val Asn Glu Glu Ile Ala Asp Glu Leu Ala Gly 65 70 75 80 ttc gag gcc gat gac cag cgc gcc atc gac cag gcc atg atc cag ctg 588 Phe Glu Ala Asp Asp Gln Arg Ala Ile Asp Gln Ala Met Ile Gln Leu 85 90 95 gac ggc acc gac aac aag gcc cgc ctg ggt gcc aac gcc atc ctg ggt 636 Asp Gly Thr Asp Asn Lys Ala Arg Leu Gly Ala Asn Ala Ile Leu Gly 100 105 110 gtc tcc atg gct gtg gcc cgc gcc gcc gcc gag tcc gcc ggc ctg ccg 684 Val Ser Met Ala Val Ala Arg Ala Ala Ala Glu Ser Ala Gly Leu Pro 115 120 125 ctc tac cgt tac gtc ggt ggc ccg aac gca cac gtg ctg ccg gtc ccg 732 Leu Tyr Arg Tyr Val Gly Gly Pro Asn Ala His Val Leu Pro Val Pro 130 135 140 atg atg aac atc ctc aac ggt ggc gcc cac gcc gac tcc ggc gtc gac 780 Met Met Asn Ile Leu Asn Gly Gly Ala His Ala Asp Ser Gly Val Asp 145 150 155 160 gtc cag gag ttc atg atc gcc ccg atc ggt gcc gag acc ttc tcc gag 828 Val Gln Glu Phe Met Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu 165 170 175 gct ctg cag gtc ggc gcc gag gtg tac cac acc ctg aag tcc gtc atc 876 Ala Leu Gln Val Gly Ala Glu Val Tyr His Thr Leu Lys Ser Val Ile 180 185 190 cag gag aag ggc ctg tcc acc ggc ctg ggc gac gag ggc ggt ttc gcc 924 Gln Glu Lys Gly Leu Ser Thr Gly Leu Gly Asp Glu Gly Gly Phe Ala 195 200 205 ccg tcc gtc gag tcc acc cgt gcg gcc ctg gac ctc atc gtc gag gcc 972 Pro Ser Val Glu Ser Thr Arg Ala Ala Leu Asp Leu Ile Val Glu Ala 210 215 220 gtc gag aag gcc ggc tac aag ctg ggc gac gat gtc gcc ctg gcc ctg 1020 Val Glu Lys Ala Gly Tyr Lys Leu Gly Asp Asp Val Ala Leu Ala Leu 225 230 235 240 gac gtc gcc tcc tcc gag ttc ttc aag gac ggc aag tac cac ttc gag 1068 Asp Val Ala Ser Ser Glu Phe Phe Lys Asp Gly Lys Tyr His Phe Glu 245 250 255 ggc aag gag ctc tcc gcc gag gag atg tcc aag gtc tac gag gag ctc 1116 Gly Lys Glu Leu Ser Ala Glu Glu Met Ser Lys Val Tyr Glu Glu Leu 260 265 270 atc aac gag tac ccg atc gtc tcc atc gag gat ccg ctg cag gag gac 1164 Ile Asn Glu Tyr Pro Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp 275 280 285 gac tgg gac ggc tac gtc gcc ctg acc gcc gcc atc ggt gac aag gtc 1212 Asp Trp Asp Gly Tyr Val Ala Leu Thr Ala Ala Ile Gly Asp Lys Val 290 295 300 cag ctg gtc ggc gac gac ttc ttc gtc acc aac ccg gcc cgc ctg aag 1260 Gln Leu Val Gly Asp Asp Phe Phe Val Thr Asn Pro Ala Arg Leu Lys 305 310 315 320 gag ggc atc gcc aag aag gcc gcc aac tcc atc ctg gtg aag gtc aac 1308 Glu Gly Ile Ala Lys Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn 325 330 335 cag atc ggt acc ctc acc gag acc ttc gac gcc gtc gag atg gcc cac 1356 Gln Ile Gly Thr Leu Thr Glu Thr Phe Asp Ala Val Glu Met Ala His 340 345 350 cgt gcg ggc tac acc tcg atg atg tcc cac cgt tcc ggt gag acc gag 1404 Arg Ala Gly Tyr Thr Ser Met Met Ser His Arg Ser Gly Glu Thr Glu 355 360 365 gac acc acc atc gcc gac ctg gct gtc gca ctg aac tgc ggc cag atc 1452 Asp Thr Thr Ile Ala Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile 370 375 380 aag acc ggt gcc ccg gcc cgc tcc gag cgc gtc gcc aag tac aac cag 1500 Lys Thr Gly Ala Pro Ala Arg Ser Glu Arg Val Ala Lys Tyr Asn Gln 385 390 395 400 ctg ctg cgc atc gag cag gag ctc ggc gac gcc gcc gtc tac gcc ggc 1548 Leu Leu Arg Ile Glu Gln Glu Leu Gly Asp Ala Ala Val Tyr Ala Gly 405 410 415 cgc tcc gcc ttc ccg cgc ttc cag aag taa ctgacagcgc tttccgacgc 1598 Arg Ser Ala Phe Pro Arg Phe Gln Lys 420 425 ccccgtgcac cccggtgcac gggggcgtcc ccgtatccgt gggggaggac accatggcat 1658 tcttcggcag acgtagggca cggccccggc tggagcccga cttcgtgcac acctggaccg 1718 gtgagcagct ggtccggatc gcggagggcg tcgacctggg caccctccgg gcggtgtacg 1778 ggcgtctcga cgccacctca cggatccact tcctcttcga cttcggtgcc ctcttctccg 1838 acgggtacct cttcgagctc tgggagcggc gtctcctgga 1878 <210> 146 <211> 425 <212> PRT <213> Corynebacterium humireducens <400> 146 Val Ala Asp Ile Ile His Val Phe Ala Arg Glu Ile Met Asp Ser Arg 1 5 10 15 Gly Asn Pro Thr Val Glu Ala Gly Val Val Leu Asp Asp Gly Thr Leu 20 25 30 Ala Thr Ala Gly Val Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala 35 40 45 His Glu Leu Arg Asp Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu 50 55 60 Lys Ala Val Glu Asn Val Asn Glu Glu Ile Ala Asp Glu Leu Ala Gly 65 70 75 80 Phe Glu Ala Asp Asp Gln Arg Ala Ile Asp Gln Ala Met Ile Gln Leu 85 90 95 Asp Gly Thr Asp Asn Lys Ala Arg Leu Gly Ala Asn Ala Ile Leu Gly 100 105 110 Val Ser Met Ala Val Ala Arg Ala Ala Ala Glu Ser Ala Gly Leu Pro 115 120 125 Leu Tyr Arg Tyr Val Gly Gly Pro Asn Ala His Val Leu Pro Val Pro 130 135 140 Met Met Asn Ile Leu Asn Gly Gly Ala His Ala Asp Ser Gly Val Asp 145 150 155 160 Val Gln Glu Phe Met Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu 165 170 175 Ala Leu Gln Val Gly Ala Glu Val Tyr His Thr Leu Lys Ser Val Ile 180 185 190 Gln Glu Lys Gly Leu Ser Thr Gly Leu Gly Asp Glu Gly Gly Phe Ala 195 200 205 Pro Ser Val Glu Ser Thr Arg Ala Ala Leu Asp Leu Ile Val Glu Ala 210 215 220 Val Glu Lys Ala Gly Tyr Lys Leu Gly Asp Asp Val Ala Leu Ala Leu 225 230 235 240 Asp Val Ala Ser Ser Glu Phe Phe Lys Asp Gly Lys Tyr His Phe Glu 245 250 255 Gly Lys Glu Leu Ser Ala Glu Glu Met Ser Lys Val Tyr Glu Glu Leu 260 265 270 Ile Asn Glu Tyr Pro Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp 275 280 285 Asp Trp Asp Gly Tyr Val Ala Leu Thr Ala Ala Ile Gly Asp Lys Val 290 295 300 Gln Leu Val Gly Asp Asp Phe Phe Val Thr Asn Pro Ala Arg Leu Lys 305 310 315 320 Glu Gly Ile Ala Lys Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn 325 330 335 Gln Ile Gly Thr Leu Thr Glu Thr Phe Asp Ala Val Glu Met Ala His 340 345 350 Arg Ala Gly Tyr Thr Ser Met Met Ser His Arg Ser Gly Glu Thr Glu 355 360 365 Asp Thr Thr Ile Ala Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile 370 375 380 Lys Thr Gly Ala Pro Ala Arg Ser Glu Arg Val Ala Lys Tyr Asn Gln 385 390 395 400 Leu Leu Arg Ile Glu Gln Glu Leu Gly Asp Ala Ala Val Tyr Ala Gly 405 410 415 Arg Ser Ala Phe Pro Arg Phe Gln Lys 420 425 <210> 147 <211> 1347 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1047) <223> gene gpmA <400> 147 tcgccgtctc cgagggggag acgggagtgc ttgtcgacgg ccacgacacc ggcgactggg 60 ccgacgccct ggagaacctc ctcgacgacg acgacacccg cctgcggatg gcgaccgacg 120 ccgtccagca cgccgcgaac ttctcgtggt cgaactccgc cgcccagctg gcggcggtgt 180 acgccgaggc cctgagcctg gaggtcgaca actgcccgca acgccgcgaa cgggccgaag 240 ggctctaaac ggggggtatt aatccgcacg ctaccggcgg aatcgtggca tattggagac 300 atg acc aac gga aag ctc att ctt ctt cgc cac ggg cag agc cag tgg 348 Met Thr Asn Gly Lys Leu Ile Leu Leu Arg His Gly Gln Ser Gln Trp 1 5 10 15 aac aaa tcg aac cag ttc acc ggc tgg gtg gac gtt gac ctg acc gag 396 Asn Lys Ser Asn Gln Phe Thr Gly Trp Val Asp Val Asp Leu Thr Glu 20 25 30 cag ggc gtc gcc gag gct gtc cgc ggc ggc gag atg ctc aag gag gct 444 Gln Gly Val Ala Glu Ala Val Arg Gly Gly Glu Met Leu Lys Glu Ala 35 40 45 ggt ctc ctg ccg gac atc gtc tac acc tcc ctg ctg cgt cgt gcc atc 492 Gly Leu Leu Pro Asp Ile Val Tyr Thr Ser Leu Leu Arg Arg Ala Ile 50 55 60 cgc acc gcg aac atc gcg ctc aac gcc gcc gac cgt cac tgg atc ccg 540 Arg Thr Ala Asn Ile Ala Leu Asn Ala Ala Asp Arg His Trp Ile Pro 65 70 75 80 gtc gtc cgc gac tgg cgt ctc aac gag cgc cac tac ggt gcg ctg cag 588 Val Val Arg Asp Trp Arg Leu Asn Glu Arg His Tyr Gly Ala Leu Gln 85 90 95 ggc ctg gac aag gcc gag acc aag gac aag tac ggc gac gag cag ttc 636 Gly Leu Asp Lys Ala Glu Thr Lys Asp Lys Tyr Gly Asp Glu Gln Phe 100 105 110 atg gcg tgg cgt cgt tcc tac gac acc ccg ccg ccg gag ctg gcc gac 684 Met Ala Trp Arg Arg Ser Tyr Asp Thr Pro Pro Pro Glu Leu Ala Asp 115 120 125 gac gcc gag tac tcc cag tcc gac gat gtg cgc tac gcc gcc ctg gag 732 Asp Ala Glu Tyr Ser Gln Ser Asp Asp Val Arg Tyr Ala Ala Leu Glu 130 135 140 aac gca ccg cgt acc gag tgc ctc ctg gac gtc gtc aag cgt ttc atc 780 Asn Ala Pro Arg Thr Glu Cys Leu Leu Asp Val Val Lys Arg Phe Ile 145 150 155 160 ccg tac tac cag gag gag atc gag ccg cgc gtc aag cgt ggc gag acc 828 Pro Tyr Tyr Gln Glu Glu Ile Glu Pro Arg Val Lys Arg Gly Glu Thr 165 170 175 gtc ctc gtc gcc gcg cac ggc aac tcc ctg cgc gcg ctg gtc aag cac 876 Val Leu Val Ala Ala His Gly Asn Ser Leu Arg Ala Leu Val Lys His 180 185 190 ctg gac aac atc tcg gac gag gac atc gcg gcc ctc aac atc ccg acc 924 Leu Asp Asn Ile Ser Asp Glu Asp Ile Ala Ala Leu Asn Ile Pro Thr 195 200 205 ggc atc ccg ctg gtc tac gag ctg gat gct gac ggc aag gtc gtc aac 972 Gly Ile Pro Leu Val Tyr Glu Leu Asp Ala Asp Gly Lys Val Val Asn 210 215 220 ccg ggc ggc acc tac ctc gac ccc gag gcg gcc gcc gcc ggt gct gcc 1020 Pro Gly Gly Thr Tyr Leu Asp Pro Glu Ala Ala Ala Ala Gly Ala Ala 225 230 235 240 gct gtc gcg gca cag ggt ggc aag tag cctgtagggc gtgtccgccg 1067 Ala Val Ala Ala Gln Gly Gly Lys 245 ttctcgcgtt cctgttgggc gtcgccgtca ccggcgtcgc ctttccggcg ttgaactggg 1127 cacgtaagcg gatagtccgc caccgttcct cggccacgct cgaggacaac caggtcacca 1187 ccgtcggcca ggtcctgcac ctggccatcc agggttcccc cacgggcgtc accgtcgtcg 1247 accgcggcgg tgaggtgatc ctgtcgaacg cccgcgccca cgagatggcc ctcgtccacg 1307 accgcaccct caacgagcag gtgtggcagg tggcggggga 1347 <210> 148 <211> 248 <212> PRT <213> Corynebacterium humireducens <400> 148 Met Thr Asn Gly Lys Leu Ile Leu Leu Arg His Gly Gln Ser Gln Trp 1 5 10 15 Asn Lys Ser Asn Gln Phe Thr Gly Trp Val Asp Val Asp Leu Thr Glu 20 25 30 Gln Gly Val Ala Glu Ala Val Arg Gly Gly Glu Met Leu Lys Glu Ala 35 40 45 Gly Leu Leu Pro Asp Ile Val Tyr Thr Ser Leu Leu Arg Arg Ala Ile 50 55 60 Arg Thr Ala Asn Ile Ala Leu Asn Ala Ala Asp Arg His Trp Ile Pro 65 70 75 80 Val Val Arg Asp Trp Arg Leu Asn Glu Arg His Tyr Gly Ala Leu Gln 85 90 95 Gly Leu Asp Lys Ala Glu Thr Lys Asp Lys Tyr Gly Asp Glu Gln Phe 100 105 110 Met Ala Trp Arg Arg Ser Tyr Asp Thr Pro Pro Pro Glu Leu Ala Asp 115 120 125 Asp Ala Glu Tyr Ser Gln Ser Asp Asp Val Arg Tyr Ala Ala Leu Glu 130 135 140 Asn Ala Pro Arg Thr Glu Cys Leu Leu Asp Val Val Lys Arg Phe Ile 145 150 155 160 Pro Tyr Tyr Gln Glu Glu Ile Glu Pro Arg Val Lys Arg Gly Glu Thr 165 170 175 Val Leu Val Ala Ala His Gly Asn Ser Leu Arg Ala Leu Val Lys His 180 185 190 Leu Asp Asn Ile Ser Asp Glu Asp Ile Ala Ala Leu Asn Ile Pro Thr 195 200 205 Gly Ile Pro Leu Val Tyr Glu Leu Asp Ala Asp Gly Lys Val Val Asn 210 215 220 Pro Gly Gly Thr Tyr Leu Asp Pro Glu Ala Ala Ala Ala Gly Ala Ala 225 230 235 240 Ala Val Ala Ala Gln Gly Gly Lys 245 <210> 149 <211> 1818 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1518) <223> gene pgk <400> 149 cgatccgcac ggctccatct tcgacgccgg cctgaccaag gtcatcggcg accaggtcaa 60 ggtcgtctcc tggtacgaca acgagtgggg ctacacctgc cagctcctgc gcctgaccga 120 gctcgtcgcc tccaagctct aggaccgcga ccccgcactt ccccggcctc cggctgagac 180 cgggggagtg cgcccctgtg gcccggggtg tgcctcggca cacgtcgggc ctcagggcta 240 tccaccccct cagatcagcc gcccctttct catctgaaga caaaagtcaa ggagacagcc 300 atg acc gtg aag act ctg cag gac ctg ctc gac gag ggc gtc gac ggc 348 Met Thr Val Lys Thr Leu Gln Asp Leu Leu Asp Glu Gly Val Asp Gly 1 5 10 15 cgc cac atc ctc gtc cgc tcc gac ttc aac gtc ccg ctc aac gac gcc 396 Arg His Ile Leu Val Arg Ser Asp Phe Asn Val Pro Leu Asn Asp Ala 20 25 30 ggt gag atc acc gac gcg ggc cgc atc acc gcc tcc ctg ccc acc ctc 444 Gly Glu Ile Thr Asp Ala Gly Arg Ile Thr Ala Ser Leu Pro Thr Leu 35 40 45 aag gcg ctc gtc gag ggt ggc gcg aag gtc atc gtc atg gcc cac ctg 492 Lys Ala Leu Val Glu Gly Gly Ala Lys Val Ile Val Met Ala His Leu 50 55 60 ggc cgc ccg aag ggc gag gtc aac ccg aag tac tcc ctg gcc ccg gtc 540 Gly Arg Pro Lys Gly Glu Val Asn Pro Lys Tyr Ser Leu Ala Pro Val 65 70 75 80 gcc gag gcc ctc tcc gag gca ctc ggc cag tac gtc gcc ctc gcc ggc 588 Ala Glu Ala Leu Ser Glu Ala Leu Gly Gln Tyr Val Ala Leu Ala Gly 85 90 95 gac gtc acc ggt gag gac gcc cac gag cgc gcc aac ggc ctg acc gag 636 Asp Val Thr Gly Glu Asp Ala His Glu Arg Ala Asn Gly Leu Thr Glu 100 105 110 ggc gac gtc ctg ctc ctc gag aac gtg cgt ttc gac gcc cgc gag acc 684 Gly Asp Val Leu Leu Leu Glu Asn Val Arg Phe Asp Ala Arg Glu Thr 115 120 125 tcc aag gac gag gcc gag cgc ggc gcc ttc gcc gac gag ctc gcc gcc 732 Ser Lys Asp Glu Ala Glu Arg Gly Ala Phe Ala Asp Glu Leu Ala Ala 130 135 140 ctg gcc gcc gac aac ggc gcc ttc gtc tcc gac ggt ttc ggt gtc gtc 780 Leu Ala Ala Asp Asn Gly Ala Phe Val Ser Asp Gly Phe Gly Val Val 145 150 155 160 cac cgt gcg cag gcc tcc gtc tac gac gtc gcc aag cgt ctc ccg gcc 828 His Arg Ala Gln Ala Ser Val Tyr Asp Val Ala Lys Arg Leu Pro Ala 165 170 175 tac gcc ggc aac ctg gtg gag aag gag atc tcc gtc ctg gag aag acc 876 Tyr Ala Gly Asn Leu Val Glu Lys Glu Ile Ser Val Leu Glu Lys Thr 180 185 190 gcc acc aac ccg gag tcc ccg tac gtc gtc gtc ctc ggc ggc tcc aag 924 Ala Thr Asn Pro Glu Ser Pro Tyr Val Val Val Leu Gly Gly Ser Lys 195 200 205 gtc tcc gac aag ctg ggc gtc atc gag gct ctg gcc ggc aag gcc gac 972 Val Ser Asp Lys Leu Gly Val Ile Glu Ala Leu Ala Gly Lys Ala Asp 210 215 220 aag atc atc atc ggt ggc ggc atg tgc tac acc ttc ctc aac gcc cag 1020 Lys Ile Ile Ile Gly Gly Gly Met Cys Tyr Thr Phe Leu Asn Ala Gln 225 230 235 240 ggc tac aac acc cag aag tcc ctg ctg cag gag gag atg atc gac acc 1068 Gly Tyr Asn Thr Gln Lys Ser Leu Leu Gln Glu Glu Met Ile Asp Thr 245 250 255 tgc aag gac ctc ctc gag cgt ttc ggc gac aag atc gtc ctc ccg gtg 1116 Cys Lys Asp Leu Leu Glu Arg Phe Gly Asp Lys Ile Val Leu Pro Val 260 265 270 gac ctc gtg gcc gcc gcc gag ttc tcc gcc gac tcc gag cac aag gtc 1164 Asp Leu Val Ala Ala Ala Glu Phe Ser Ala Asp Ser Glu His Lys Val 275 280 285 gtc gcc ctc gac gag atc ccg gag ggc tgg ctg tcc ccg gac atc ggc 1212 Val Ala Leu Asp Glu Ile Pro Glu Gly Trp Leu Ser Pro Asp Ile Gly 290 295 300 ccg gag tcc gtc aag aag ttc gcc gac gtc ctc gcc acc tcc aag acc 1260 Pro Glu Ser Val Lys Lys Phe Ala Asp Val Leu Ala Thr Ser Lys Thr 305 310 315 320 gtc ttc tgg aac ggc ccg atg ggt gtc ttc gag atg gca ccg ttc tcc 1308 Val Phe Trp Asn Gly Pro Met Gly Val Phe Glu Met Ala Pro Phe Ser 325 330 335 aag ggc acc gag ggc gtc gcc aag gcc atc atc gac gcg acc gcg aac 1356 Lys Gly Thr Glu Gly Val Ala Lys Ala Ile Ile Asp Ala Thr Ala Asn 340 345 350 aac ggt tcc ttc tcc gtc gtc ggt ggc ggc gac tcc gca gcc tcc gtc 1404 Asn Gly Ser Phe Ser Val Val Gly Gly Gly Asp Ser Ala Ala Ser Val 355 360 365 cgc gtg ctc ggc ctg gac gag gac ggc ttc tcc cac atc tcc acc ggc 1452 Arg Val Leu Gly Leu Asp Glu Asp Gly Phe Ser His Ile Ser Thr Gly 370 375 380 ggc ggc gcc tcc ctc gag ttc ctc gag ggc aag gag ctc ccg ggc gtt 1500 Gly Gly Ala Ser Leu Glu Phe Leu Glu Gly Lys Glu Leu Pro Gly Val 385 390 395 400 tcc gtc ctg gac cgc taa gcacttacca cccactctga aaggagtgcc 1548 Ser Val Leu Asp Arg 405 ccacccatgg cacgcacccc cctcatcgcc ggcaactgga agatgaacct ggaccaccac 1608 caggccatcg ccaccgtgca gaagctcgac ttcgccctgc cgaaggagta ctacgagaag 1668 gtcgacgtcg ccgtcaccgt cccgttcacc gacctccgtt ccgtgcagac gctcgtcgac 1728 ggggacaagc tcaaggtcac ctacggtgcc caggacgtct cccggcacga gtccggcgcg 1788 tacaccggtg aggtctccgc tcagatgctc 1818 <210> 150 <211> 405 <212> PRT <213> Corynebacterium humireducens <400> 150 Met Thr Val Lys Thr Leu Gln Asp Leu Leu Asp Glu Gly Val Asp Gly 1 5 10 15 Arg His Ile Leu Val Arg Ser Asp Phe Asn Val Pro Leu Asn Asp Ala 20 25 30 Gly Glu Ile Thr Asp Ala Gly Arg Ile Thr Ala Ser Leu Pro Thr Leu 35 40 45 Lys Ala Leu Val Glu Gly Gly Ala Lys Val Ile Val Met Ala His Leu 50 55 60 Gly Arg Pro Lys Gly Glu Val Asn Pro Lys Tyr Ser Leu Ala Pro Val 65 70 75 80 Ala Glu Ala Leu Ser Glu Ala Leu Gly Gln Tyr Val Ala Leu Ala Gly 85 90 95 Asp Val Thr Gly Glu Asp Ala His Glu Arg Ala Asn Gly Leu Thr Glu 100 105 110 Gly Asp Val Leu Leu Leu Glu Asn Val Arg Phe Asp Ala Arg Glu Thr 115 120 125 Ser Lys Asp Glu Ala Glu Arg Gly Ala Phe Ala Asp Glu Leu Ala Ala 130 135 140 Leu Ala Ala Asp Asn Gly Ala Phe Val Ser Asp Gly Phe Gly Val Val 145 150 155 160 His Arg Ala Gln Ala Ser Val Tyr Asp Val Ala Lys Arg Leu Pro Ala 165 170 175 Tyr Ala Gly Asn Leu Val Glu Lys Glu Ile Ser Val Leu Glu Lys Thr 180 185 190 Ala Thr Asn Pro Glu Ser Pro Tyr Val Val Val Leu Gly Gly Ser Lys 195 200 205 Val Ser Asp Lys Leu Gly Val Ile Glu Ala Leu Ala Gly Lys Ala Asp 210 215 220 Lys Ile Ile Ile Gly Gly Gly Met Cys Tyr Thr Phe Leu Asn Ala Gln 225 230 235 240 Gly Tyr Asn Thr Gln Lys Ser Leu Leu Gln Glu Glu Met Ile Asp Thr 245 250 255 Cys Lys Asp Leu Leu Glu Arg Phe Gly Asp Lys Ile Val Leu Pro Val 260 265 270 Asp Leu Val Ala Ala Ala Glu Phe Ser Ala Asp Ser Glu His Lys Val 275 280 285 Val Ala Leu Asp Glu Ile Pro Glu Gly Trp Leu Ser Pro Asp Ile Gly 290 295 300 Pro Glu Ser Val Lys Lys Phe Ala Asp Val Leu Ala Thr Ser Lys Thr 305 310 315 320 Val Phe Trp Asn Gly Pro Met Gly Val Phe Glu Met Ala Pro Phe Ser 325 330 335 Lys Gly Thr Glu Gly Val Ala Lys Ala Ile Ile Asp Ala Thr Ala Asn 340 345 350 Asn Gly Ser Phe Ser Val Val Gly Gly Gly Asp Ser Ala Ala Ser Val 355 360 365 Arg Val Leu Gly Leu Asp Glu Asp Gly Phe Ser His Ile Ser Thr Gly 370 375 380 Gly Gly Ala Ser Leu Glu Phe Leu Glu Gly Lys Glu Leu Pro Gly Val 385 390 395 400 Ser Val Leu Asp Arg 405 <210> 151 <211> 2058 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1758) <223> gene glyceraldehyde_3_phopshatdehydrogenase 1 <400> 151 gcggcgggcg ggcgcagcgt ggcgagccgg gccttcagcg gccgcgtcgc ccgcggactg 60 gaaacggact tcacccggaa caacccggac atcccgccga tctaccccta cctcgtgtcg 120 ctgcaggccg cggaacggcg ggaacgcccg cgggaactgg cctactgcct cgtcggggtg 180 ggcgtcgaga agctcggggg tggccccgcg gcggggattc tggctcgact cgcgcctgaa 240 caggcaattt cacgctaaag tcgtggggac gtttcccttt actttcggag gtaaaaagca 300 gtg acc gcc cac gcg cag cct gag cac aac aac tgg aac gag aag atc 348 Val Thr Ala His Ala Gln Pro Glu His Asn Asn Trp Asn Glu Lys Ile 1 5 10 15 gcc ctc gct cag gag atg ctg ccg ctg atc ggc cag ctc cac cgc aac 396 Ala Leu Ala Gln Glu Met Leu Pro Leu Ile Gly Gln Leu His Arg Asn 20 25 30 aac gat gtc gtc gtc tcc atc ttc gga cga ctg ctt gtc ggc gcg acg 444 Asn Asp Val Val Val Ser Ile Phe Gly Arg Leu Leu Val Gly Ala Thr 35 40 45 gac atc gac atc atc aag gcg cac cgt tac gcg cgc cgc gtc gcc gac 492 Asp Ile Asp Ile Ile Lys Ala His Arg Tyr Ala Arg Arg Val Ala Asp 50 55 60 cgt gag atg aag ctc gat cag acc ctc ccg gtc ctg cgc gag ctc gtc 540 Arg Glu Met Lys Leu Asp Gln Thr Leu Pro Val Leu Arg Glu Leu Val 65 70 75 80 gac atg gac ctc ggc acc gcc tcc atc gac ctg ggc cgc ctg gcc acc 588 Asp Met Asp Leu Gly Thr Ala Ser Ile Asp Leu Gly Arg Leu Ala Thr 85 90 95 ggc ttc gag aag tcc ggc ggc acc gac ctg cgt gcc tac ctg gac gag 636 Gly Phe Glu Lys Ser Gly Gly Thr Asp Leu Arg Ala Tyr Leu Asp Glu 100 105 110 cag ctc gcc gac atc gtc ggc aag cgc tcc gag acc gag ccg cgc gac 684 Gln Leu Ala Asp Ile Val Gly Lys Arg Ser Glu Thr Glu Pro Arg Asp 115 120 125 gtc gtc ctc tac ggc ttc ggc cgc atc ggc cgc ctc ctg gcc cgc atc 732 Val Val Leu Tyr Gly Phe Gly Arg Ile Gly Arg Leu Leu Ala Arg Ile 130 135 140 ctc atc tcc cgt gag gcc acc tac ggc ggc gtc cgc ctc cgc gcg gtg 780 Leu Ile Ser Arg Glu Ala Thr Tyr Gly Gly Val Arg Leu Arg Ala Val 145 150 155 160 gtg gta cgc aag aac tcc gag gac gac atc gtc aag cgt gcc tcc ctc 828 Val Val Arg Lys Asn Ser Glu Asp Asp Ile Val Lys Arg Ala Ser Leu 165 170 175 ctg cgc cgt gac tcc gtg cac ggc gcc ttc gac ggc acg atc acc gtc 876 Leu Arg Arg Asp Ser Val His Gly Ala Phe Asp Gly Thr Ile Thr Val 180 185 190 gac aag gag aac aac gtc atc tgg gcc aac ggc acc aag atc cag atc 924 Asp Lys Glu Asn Asn Val Ile Trp Ala Asn Gly Thr Lys Ile Gln Ile 195 200 205 atc tac tcc aac tcc ccg gcc gag gtc gac tac acc gcc tac ggc atc 972 Ile Tyr Ser Asn Ser Pro Ala Glu Val Asp Tyr Thr Ala Tyr Gly Ile 210 215 220 aat gac gcc atc gtg gtg gac aac acc ggc atc tgg cgc gac cgc gag 1020 Asn Asp Ala Ile Val Val Asp Asn Thr Gly Ile Trp Arg Asp Arg Glu 225 230 235 240 gga ctg tcc cag cac ctg cag tcc aag ggc gtc tcc cgc gtc gtc ctc 1068 Gly Leu Ser Gln His Leu Gln Ser Lys Gly Val Ser Arg Val Val Leu 245 250 255 acc gcc ccg ggc aag ggc gac ctg ccg aac atc gtc tac ggc atc aac 1116 Thr Ala Pro Gly Lys Gly Asp Leu Pro Asn Ile Val Tyr Gly Ile Asn 260 265 270 gac gac atg atc acc gac gac gag cag atc atc acc gcc gcc tcc tgc 1164 Asp Asp Met Ile Thr Asp Asp Glu Gln Ile Ile Thr Ala Ala Ser Cys 275 280 285 acc acc aac ggc atc acc ccg gtc ctg aag gtc atc aac gac cgc tac 1212 Thr Thr Asn Gly Ile Thr Pro Val Leu Lys Val Ile Asn Asp Arg Tyr 290 295 300 ggc gtc gag cac ggt cac gtc gag acc gtc cac tcc ttc acc aac gac 1260 Gly Val Glu His Gly His Val Glu Thr Val His Ser Phe Thr Asn Asp 305 310 315 320 cag aac ctc atc gac aac ttc cac aag ggc acc cgc cgt ggc cgt gcc 1308 Gln Asn Leu Ile Asp Asn Phe His Lys Gly Thr Arg Arg Gly Arg Ala 325 330 335 gcc tcc ctc aac atg gtc ctc acc gag acc ggc gcc gcc aag gcc gtc 1356 Ala Ser Leu Asn Met Val Leu Thr Glu Thr Gly Ala Ala Lys Ala Val 340 345 350 tcc aag gcc ctg ccg gag ttc gag ggc aag ctc acc ggc aac gcc atc 1404 Ser Lys Ala Leu Pro Glu Phe Glu Gly Lys Leu Thr Gly Asn Ala Ile 355 360 365 cgc gtc ccc acc ccg aac gtc tcc atg gcg gtg ctc aac ctc acc ctg 1452 Arg Val Pro Thr Pro Asn Val Ser Met Ala Val Leu Asn Leu Thr Leu 370 375 380 gag aag gag gtc gac cgc gag gag gtc aac aac ttc ctg cgc aag gtc 1500 Glu Lys Glu Val Asp Arg Glu Glu Val Asn Asn Phe Leu Arg Lys Val 385 390 395 400 tcc ctg cac tcc gac ctg cgc cag cag atc tcc tac atc aac tcc ccg 1548 Ser Leu His Ser Asp Leu Arg Gln Gln Ile Ser Tyr Ile Asn Ser Pro 405 410 415 gag gtc gtg tcc acc gac ttc gtc ggc agc acc cac gcc ggc atc gtc 1596 Glu Val Val Ser Thr Asp Phe Val Gly Ser Thr His Ala Gly Ile Val 420 425 430 gac ggc ctg gcc acc atc gcc tcc ggc aag cac ctc gtg ctc tac gtc 1644 Asp Gly Leu Ala Thr Ile Ala Ser Gly Lys His Leu Val Leu Tyr Val 435 440 445 tgg tac gac aac gag ttc ggc tac tcc aac cag gtc atc cgc atc gtc 1692 Trp Tyr Asp Asn Glu Phe Gly Tyr Ser Asn Gln Val Ile Arg Ile Val 450 455 460 gag gag gtc gcc ggc gcc cgc ccg gag atc tac ccg cgt cgc ctg gag 1740 Glu Glu Val Ala Gly Ala Arg Pro Glu Ile Tyr Pro Arg Arg Leu Glu 465 470 475 480 gcc tct gag ctg aag tag cttttccagg atcagcttca agctgccgca 1788 Ala Ser Glu Leu Lys 485 gcgacaggcc cggttctcca cgtggagaac cgggcctgtc gcattcagga ggggcggcag 1848 cggcacaggc agatgaagaa tcgtcgtcgg tgtgtccgct cactgcgcct ggtgttaggc 1908 ccgtgctcta cacccgcccg ctgccggtta cggcaatcat ggccaggagc aggtgctgac 1968 cggtctgaca ggcgcctacg ctcacccagt caactgctga ctgggacatt gacttgagga 2028 tgcgtgcttc ccggcgtatg tggtcccttg 2058 <210> 152 <211> 485 <212> PRT <213> Corynebacterium humireducens <400> 152 Val Thr Ala His Ala Gln Pro Glu His Asn Asn Trp Asn Glu Lys Ile 1 5 10 15 Ala Leu Ala Gln Glu Met Leu Pro Leu Ile Gly Gln Leu His Arg Asn 20 25 30 Asn Asp Val Val Val Ser Ile Phe Gly Arg Leu Leu Val Gly Ala Thr 35 40 45 Asp Ile Asp Ile Ile Lys Ala His Arg Tyr Ala Arg Arg Val Ala Asp 50 55 60 Arg Glu Met Lys Leu Asp Gln Thr Leu Pro Val Leu Arg Glu Leu Val 65 70 75 80 Asp Met Asp Leu Gly Thr Ala Ser Ile Asp Leu Gly Arg Leu Ala Thr 85 90 95 Gly Phe Glu Lys Ser Gly Gly Thr Asp Leu Arg Ala Tyr Leu Asp Glu 100 105 110 Gln Leu Ala Asp Ile Val Gly Lys Arg Ser Glu Thr Glu Pro Arg Asp 115 120 125 Val Val Leu Tyr Gly Phe Gly Arg Ile Gly Arg Leu Leu Ala Arg Ile 130 135 140 Leu Ile Ser Arg Glu Ala Thr Tyr Gly Gly Val Arg Leu Arg Ala Val 145 150 155 160 Val Val Arg Lys Asn Ser Glu Asp Asp Ile Val Lys Arg Ala Ser Leu 165 170 175 Leu Arg Arg Asp Ser Val His Gly Ala Phe Asp Gly Thr Ile Thr Val 180 185 190 Asp Lys Glu Asn Asn Val Ile Trp Ala Asn Gly Thr Lys Ile Gln Ile 195 200 205 Ile Tyr Ser Asn Ser Pro Ala Glu Val Asp Tyr Thr Ala Tyr Gly Ile 210 215 220 Asn Asp Ala Ile Val Val Asp Asn Thr Gly Ile Trp Arg Asp Arg Glu 225 230 235 240 Gly Leu Ser Gln His Leu Gln Ser Lys Gly Val Ser Arg Val Val Leu 245 250 255 Thr Ala Pro Gly Lys Gly Asp Leu Pro Asn Ile Val Tyr Gly Ile Asn 260 265 270 Asp Asp Met Ile Thr Asp Asp Glu Gln Ile Ile Thr Ala Ala Ser Cys 275 280 285 Thr Thr Asn Gly Ile Thr Pro Val Leu Lys Val Ile Asn Asp Arg Tyr 290 295 300 Gly Val Glu His Gly His Val Glu Thr Val His Ser Phe Thr Asn Asp 305 310 315 320 Gln Asn Leu Ile Asp Asn Phe His Lys Gly Thr Arg Arg Gly Arg Ala 325 330 335 Ala Ser Leu Asn Met Val Leu Thr Glu Thr Gly Ala Ala Lys Ala Val 340 345 350 Ser Lys Ala Leu Pro Glu Phe Glu Gly Lys Leu Thr Gly Asn Ala Ile 355 360 365 Arg Val Pro Thr Pro Asn Val Ser Met Ala Val Leu Asn Leu Thr Leu 370 375 380 Glu Lys Glu Val Asp Arg Glu Glu Val Asn Asn Phe Leu Arg Lys Val 385 390 395 400 Ser Leu His Ser Asp Leu Arg Gln Gln Ile Ser Tyr Ile Asn Ser Pro 405 410 415 Glu Val Val Ser Thr Asp Phe Val Gly Ser Thr His Ala Gly Ile Val 420 425 430 Asp Gly Leu Ala Thr Ile Ala Ser Gly Lys His Leu Val Leu Tyr Val 435 440 445 Trp Tyr Asp Asn Glu Phe Gly Tyr Ser Asn Gln Val Ile Arg Ile Val 450 455 460 Glu Glu Val Ala Gly Ala Arg Pro Glu Ile Tyr Pro Arg Arg Leu Glu 465 470 475 480 Ala Ser Glu Leu Lys 485 <210> 153 <211> 1605 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1305) <223> gene glyceraldehyde_3_phopshatdehydrogenase 2 <400> 153 gctccattcg ggggtgaatc gggcatgtgt gaccgatatt attcgccggt gtccggagga 60 ccgcccggtg cccggccgcc tgcgaccccc cgttgacaac gactttcggg aagtggggcg 120 gaccggaatg tgagggcaaa cataatcgga aatccgcgga tgtctgaaaa tgtctgattc 180 ttcgctgcac agcggggtaa gtgccgctaa actgtcgctt gatgggccgt gaacgccagt 240 cactacgacg gacagtgcgg tcacatccgg acacgtgtac tcaaacccga ggagaaaatt 300 gtg acc atc cgc gtt ggc atc aac ggc ttc ggc cgt atc ggc cgt aac 348 Val Thr Ile Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn 1 5 10 15 ttc tac cgc gca gtc ctg gag cgc agc gag gcc atc gag atc gtc gcc 396 Phe Tyr Arg Ala Val Leu Glu Arg Ser Glu Ala Ile Glu Ile Val Ala 20 25 30 gtc aac gac ctc acc gac aac aag acg ctg tcg acc ctc ctg aag tac 444 Val Asn Asp Leu Thr Asp Asn Lys Thr Leu Ser Thr Leu Leu Lys Tyr 35 40 45 gac tcc atc ctc ggc aag ctg ccg ggc gag gtc tcg ttc gac gac gac 492 Asp Ser Ile Leu Gly Lys Leu Pro Gly Glu Val Ser Phe Asp Asp Asp 50 55 60 tcc atc acc tac gac ggc aag acc atc gcc gtc tcc gct gag cgc gac 540 Ser Ile Thr Tyr Asp Gly Lys Thr Ile Ala Val Ser Ala Glu Arg Asp 65 70 75 80 ccg aag aac ctg gac tgg gcc gcc cac aac gtg gac atc gtc atc gag 588 Pro Lys Asn Leu Asp Trp Ala Ala His Asn Val Asp Ile Val Ile Glu 85 90 95 tcc acc ggc ttc ttc acc gac gcc gag gca gcc aag gcc cac atc gag 636 Ser Thr Gly Phe Phe Thr Asp Ala Glu Ala Ala Lys Ala His Ile Glu 100 105 110 gcc ggc gcc aag aag gtc atc atc tcc gcc ccg gcc tcc aac gag gac 684 Ala Gly Ala Lys Lys Val Ile Ile Ser Ala Pro Ala Ser Asn Glu Asp 115 120 125 gcg acc ttc gtc tac ggc gtc aac cac cag gac tac gac ccg gag aac 732 Ala Thr Phe Val Tyr Gly Val Asn His Gln Asp Tyr Asp Pro Glu Asn 130 135 140 cac aac gtc atc tcc gcc gca tcc tgc acc acc aac tgc ctg gcc ccg 780 His Asn Val Ile Ser Ala Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro 145 150 155 160 atg gcc aag gtc ctc aac gac aag ctc ggc atc gag cgc ggc ctg atg 828 Met Ala Lys Val Leu Asn Asp Lys Leu Gly Ile Glu Arg Gly Leu Met 165 170 175 acc acc atc cac gcc tac acc ggt gac cag cgt ctg cac gac gcc ccg 876 Thr Thr Ile His Ala Tyr Thr Gly Asp Gln Arg Leu His Asp Ala Pro 180 185 190 cac aag gac ctg cgc cgc gcc cgc gcc gcc gcc gtc aac atc gtg ccc 924 His Lys Asp Leu Arg Arg Ala Arg Ala Ala Ala Val Asn Ile Val Pro 195 200 205 acc tcc acc ggt gcc gcc aag gcc gtc tcc ctg gtc ctg ccg gag ctg 972 Thr Ser Thr Gly Ala Ala Lys Ala Val Ser Leu Val Leu Pro Glu Leu 210 215 220 aag ggc aag ctg gac ggc tac gca ctg cgc gtc ccg acc atc acc ggt 1020 Lys Gly Lys Leu Asp Gly Tyr Ala Leu Arg Val Pro Thr Ile Thr Gly 225 230 235 240 tcc gcc acc gac ctc acc ttc acc gcc tcc cgc gag acc tct gtc gag 1068 Ser Ala Thr Asp Leu Thr Phe Thr Ala Ser Arg Glu Thr Ser Val Glu 245 250 255 gag gtc aac gcc ttc atc aag gag gcc gcg cag ggc gag ttc ggt gag 1116 Glu Val Asn Ala Phe Ile Lys Glu Ala Ala Gln Gly Glu Phe Gly Glu 260 265 270 acc ctg gcc tac acc gag gat ccg atc gtc tcc acc gac atc gtc acc 1164 Thr Leu Ala Tyr Thr Glu Asp Pro Ile Val Ser Thr Asp Ile Val Thr 275 280 285 gat ccg cac ggc tcc atc ttc gac gcc ggc ctg acc aag gtc atc ggc 1212 Asp Pro His Gly Ser Ile Phe Asp Ala Gly Leu Thr Lys Val Ile Gly 290 295 300 gac cag gtc aag gtc gtc tcc tgg tac gac aac gag tgg ggc tac acc 1260 Asp Gln Val Lys Val Val Ser Trp Tyr Asp Asn Glu Trp Gly Tyr Thr 305 310 315 320 tgc cag ctc ctg cgc ctg acc gag ctc gtc gcc tcc aag ctc tag 1305 Cys Gln Leu Leu Arg Leu Thr Glu Leu Val Ala Ser Lys Leu 325 330 gaccgcgacc ccgcacttcc ccggcctccg gctgagaccg ggggagtgcg cccctgtggc 1365 ccggggtgtg cctcggcaca cgtcgggcct cagggctatc caccccctca gatcagccgc 1425 ccctttctca tctgaagaca aaagtcaagg agacagccat gaccgtgaag actctgcagg 1485 acctgctcga cgagggcgtc gacggccgcc acatcctcgt ccgctccgac ttcaacgtcc 1545 cgctcaacga cgccggtgag atcaccgacg cgggccgcat caccgcctcc ctgcccaccc 1605 <210> 154 <211> 334 <212> PRT <213> Corynebacterium humireducens <400> 154 Val Thr Ile Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn 1 5 10 15 Phe Tyr Arg Ala Val Leu Glu Arg Ser Glu Ala Ile Glu Ile Val Ala 20 25 30 Val Asn Asp Leu Thr Asp Asn Lys Thr Leu Ser Thr Leu Leu Lys Tyr 35 40 45 Asp Ser Ile Leu Gly Lys Leu Pro Gly Glu Val Ser Phe Asp Asp Asp 50 55 60 Ser Ile Thr Tyr Asp Gly Lys Thr Ile Ala Val Ser Ala Glu Arg Asp 65 70 75 80 Pro Lys Asn Leu Asp Trp Ala Ala His Asn Val Asp Ile Val Ile Glu 85 90 95 Ser Thr Gly Phe Phe Thr Asp Ala Glu Ala Ala Lys Ala His Ile Glu 100 105 110 Ala Gly Ala Lys Lys Val Ile Ile Ser Ala Pro Ala Ser Asn Glu Asp 115 120 125 Ala Thr Phe Val Tyr Gly Val Asn His Gln Asp Tyr Asp Pro Glu Asn 130 135 140 His Asn Val Ile Ser Ala Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro 145 150 155 160 Met Ala Lys Val Leu Asn Asp Lys Leu Gly Ile Glu Arg Gly Leu Met 165 170 175 Thr Thr Ile His Ala Tyr Thr Gly Asp Gln Arg Leu His Asp Ala Pro 180 185 190 His Lys Asp Leu Arg Arg Ala Arg Ala Ala Ala Val Asn Ile Val Pro 195 200 205 Thr Ser Thr Gly Ala Ala Lys Ala Val Ser Leu Val Leu Pro Glu Leu 210 215 220 Lys Gly Lys Leu Asp Gly Tyr Ala Leu Arg Val Pro Thr Ile Thr Gly 225 230 235 240 Ser Ala Thr Asp Leu Thr Phe Thr Ala Ser Arg Glu Thr Ser Val Glu 245 250 255 Glu Val Asn Ala Phe Ile Lys Glu Ala Ala Gln Gly Glu Phe Gly Glu 260 265 270 Thr Leu Ala Tyr Thr Glu Asp Pro Ile Val Ser Thr Asp Ile Val Thr 275 280 285 Asp Pro His Gly Ser Ile Phe Asp Ala Gly Leu Thr Lys Val Ile Gly 290 295 300 Asp Gln Val Lys Val Val Ser Trp Tyr Asp Asn Glu Trp Gly Tyr Thr 305 310 315 320 Cys Gln Leu Leu Arg Leu Thr Glu Leu Val Ala Ser Lys Leu 325 330 <210> 155 <211> 1380 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1080) <223> gene tpiA <400> 155 aagaccgtct tctggaacgg cccgatgggt gtcttcgaga tggcaccgtt ctccaagggc 60 accgagggcg tcgccaaggc catcatcgac gcgaccgcga acaacggttc cttctccgtc 120 gtcggtggcg gcgactccgc agcctccgtc cgcgtgctcg gcctggacga ggacggcttc 180 tcccacatct ccaccggcgg cggcgcctcc ctcgagttcc tcgagggcaa ggagctcccg 240 ggcgtttccg tcctggaccg ctaagcactt accacccact ctgaaaggag tgccccaccc 300 atg gca cgc acc ccc ctc atc gcc ggc aac tgg aag atg aac ctg gac 348 Met Ala Arg Thr Pro Leu Ile Ala Gly Asn Trp Lys Met Asn Leu Asp 1 5 10 15 cac cac cag gcc atc gcc acc gtg cag aag ctc gac ttc gcc ctg ccg 396 His His Gln Ala Ile Ala Thr Val Gln Lys Leu Asp Phe Ala Leu Pro 20 25 30 aag gag tac tac gag aag gtc gac gtc gcc gtc acc gtc ccg ttc acc 444 Lys Glu Tyr Tyr Glu Lys Val Asp Val Ala Val Thr Val Pro Phe Thr 35 40 45 gac ctc cgt tcc gtg cag acg ctc gtc gac ggg gac aag ctc aag gtc 492 Asp Leu Arg Ser Val Gln Thr Leu Val Asp Gly Asp Lys Leu Lys Val 50 55 60 acc tac ggt gcc cag gac gtc tcc cgg cac gag tcc ggc gcg tac acc 540 Thr Tyr Gly Ala Gln Asp Val Ser Arg His Glu Ser Gly Ala Tyr Thr 65 70 75 80 ggt gag gtc tcc gct cag atg ctc gcc aag ctc ggc tgc acc tgg gtc 588 Gly Glu Val Ser Ala Gln Met Leu Ala Lys Leu Gly Cys Thr Trp Val 85 90 95 gtc gtc ggc cac tcc gag cgc cgc gag tac cac aac gag tcc gac gag 636 Val Val Gly His Ser Glu Arg Arg Glu Tyr His Asn Glu Ser Asp Glu 100 105 110 ctg gtc gcc gcg aag gcc gct gcc gcc ctc cgc cac ggc atg agc ccg 684 Leu Val Ala Ala Lys Ala Ala Ala Ala Leu Arg His Gly Met Ser Pro 115 120 125 atc gtg tgc gtc ggc gag ccg ctg gag atc cgc gag gcc ggc acc cac 732 Ile Val Cys Val Gly Glu Pro Leu Glu Ile Arg Glu Ala Gly Thr His 130 135 140 gtc gac tac gtc gtc gag cag acc cgt gcc tcc ctg gcc ggc ctg tcc 780 Val Asp Tyr Val Val Glu Gln Thr Arg Ala Ser Leu Ala Gly Leu Ser 145 150 155 160 gcc gag gac ctg tcc aac acc gtc atc gcc tac gag ccc gtg tgg gcc 828 Ala Glu Asp Leu Ser Asn Thr Val Ile Ala Tyr Glu Pro Val Trp Ala 165 170 175 atc ggc acc ggc aag gtc gcc tcc gcg gcc gat gcc cag gag gtc tgt 876 Ile Gly Thr Gly Lys Val Ala Ser Ala Ala Asp Ala Gln Glu Val Cys 180 185 190 gcg gcg atc cgc ggt ctc gtc cgc gag ctg gcc ggt gac gag gtc gcc 924 Ala Ala Ile Arg Gly Leu Val Arg Glu Leu Ala Gly Asp Glu Val Ala 195 200 205 gac ggc atc cgc atc ctc tac ggc ggt tcc gtc aag acc gac tcg gtc 972 Asp Gly Ile Arg Ile Leu Tyr Gly Gly Ser Val Lys Thr Asp Ser Val 210 215 220 gcc gag atc gtc ggt cag ccc gac gtc gac ggt ggt ctc gtc ggt ggc 1020 Ala Glu Ile Val Gly Gln Pro Asp Val Asp Gly Gly Leu Val Gly Gly 225 230 235 240 gcc tcg ctc gac ggc gag gag ttc gcc aag ctg gcg gcc gcc gcg gcg 1068 Ala Ser Leu Asp Gly Glu Glu Phe Ala Lys Leu Ala Ala Ala Ala Ala 245 250 255 tcc gtc gtc tag catcacctca tccaccggcc ccgggcactg aacattcagt 1120 Ser Val Val gcccggggcc ggtgtccttt ctccggggaa tatggtgcgc aatccccacg cacggccgtc 1180 atatgcgtca tgatggggag gcgaaccgca tccccgcctt cttcgagtac gaacagggag 1240 acactcaatg accgtccgcg atcatctgca ggaagacatc cgctacctcg gccgtatcct 1300 cggccaggtc atcgcggaac aggagggcga ggacgtgttc aacctcgtcg agcacgcccg 1360 ccagcaggcc ttcgaggtgg 1380 <210> 156 <211> 259 <212> PRT <213> Corynebacterium humireducens <400> 156 Met Ala Arg Thr Pro Leu Ile Ala Gly Asn Trp Lys Met Asn Leu Asp 1 5 10 15 His His Gln Ala Ile Ala Thr Val Gln Lys Leu Asp Phe Ala Leu Pro 20 25 30 Lys Glu Tyr Tyr Glu Lys Val Asp Val Ala Val Thr Val Pro Phe Thr 35 40 45 Asp Leu Arg Ser Val Gln Thr Leu Val Asp Gly Asp Lys Leu Lys Val 50 55 60 Thr Tyr Gly Ala Gln Asp Val Ser Arg His Glu Ser Gly Ala Tyr Thr 65 70 75 80 Gly Glu Val Ser Ala Gln Met Leu Ala Lys Leu Gly Cys Thr Trp Val 85 90 95 Val Val Gly His Ser Glu Arg Arg Glu Tyr His Asn Glu Ser Asp Glu 100 105 110 Leu Val Ala Ala Lys Ala Ala Ala Ala Leu Arg His Gly Met Ser Pro 115 120 125 Ile Val Cys Val Gly Glu Pro Leu Glu Ile Arg Glu Ala Gly Thr His 130 135 140 Val Asp Tyr Val Val Glu Gln Thr Arg Ala Ser Leu Ala Gly Leu Ser 145 150 155 160 Ala Glu Asp Leu Ser Asn Thr Val Ile Ala Tyr Glu Pro Val Trp Ala 165 170 175 Ile Gly Thr Gly Lys Val Ala Ser Ala Ala Asp Ala Gln Glu Val Cys 180 185 190 Ala Ala Ile Arg Gly Leu Val Arg Glu Leu Ala Gly Asp Glu Val Ala 195 200 205 Asp Gly Ile Arg Ile Leu Tyr Gly Gly Ser Val Lys Thr Asp Ser Val 210 215 220 Ala Glu Ile Val Gly Gln Pro Asp Val Asp Gly Gly Leu Val Gly Gly 225 230 235 240 Ala Ser Leu Asp Gly Glu Glu Phe Ala Lys Leu Ala Ala Ala Ala Ala 245 250 255 Ser Val Val <210> 157 <211> 1632 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1332) <223> gene frutcosebisphosphataldolase <400> 157 ccgactggac cgccgacgcc cgcctgccgc acaacttcgg cctggcgccg tcgtcactgc 60 cggaggccct cggcgtcctg cgtatcgacg aacgcgatct ctccgtccag ctctccggct 120 ggatgctgct ggaggccgcc gcccgggtgt ccacctacgc ggagacccag cggtcctgat 180 ccccgcgcgc ggggcggggg gggaggtgac gcgggttttc cgccctctgt tgcgagcgcg 240 ggaggccaaa gggaggcata ctggtatacg gacctgtttg ctaacccgga ggatcaccct 300 atg cct atc gcg act ccc gaa atc tac aac gag atg ctc gac agg gcc 348 Met Pro Ile Ala Thr Pro Glu Ile Tyr Asn Glu Met Leu Asp Arg Ala 1 5 10 15 aag gag ggc ggc tac gcc ttc ccg gcc atc aac tgc acc tcc tcg gag 396 Lys Glu Gly Gly Tyr Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu 20 25 30 acc atc aac gcc gct ctc cgc ggc ttc gcc gag gcc gag tcc gac ggc 444 Thr Ile Asn Ala Ala Leu Arg Gly Phe Ala Glu Ala Glu Ser Asp Gly 35 40 45 atc atc cag ttc tcc acc ggt ggc gcc gaa ttc ggc tcc ggt ctg tcc 492 Ile Ile Gln Phe Ser Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ser 50 55 60 gtg aag aac aag gtc gcc ggc gcc gtc gcc ctg gcc tcc ttc gcg cat 540 Val Lys Asn Lys Val Ala Gly Ala Val Ala Leu Ala Ser Phe Ala His 65 70 75 80 gag gcc gcc aag cac tac ggc gtc aac gtc gcc ctg cac acc gac cac 588 Glu Ala Ala Lys His Tyr Gly Val Asn Val Ala Leu His Thr Asp His 85 90 95 tgc cag aag gag gtg ctc gac gag ttc gtg cgc ccg ctg ctg gcc atc 636 Cys Gln Lys Glu Val Leu Asp Glu Phe Val Arg Pro Leu Leu Ala Ile 100 105 110 tcc cag gag cgc gtc gac gcc ggt gag ctc ccg ctc ttc cag tcc cac 684 Ser Gln Glu Arg Val Asp Ala Gly Glu Leu Pro Leu Phe Gln Ser His 115 120 125 atg tgg gac ggt tcc gct gtc ccg atc gac gag aac ctg gag atc gcg 732 Met Trp Asp Gly Ser Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala 130 135 140 aag gag ctg ctg gag aag tcc cgc aag gcg aac att atc ctc gag atc 780 Lys Glu Leu Leu Glu Lys Ser Arg Lys Ala Asn Ile Ile Leu Glu Ile 145 150 155 160 gag atc ggt gtc gtc ggc ggc gag gag gac ggt gtc gag gcc aag gcc 828 Glu Ile Gly Val Val Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala 165 170 175 ggc gcc aac ctc tac acc acc gag gag gac ttc gag aag acc atc gac 876 Gly Ala Asn Leu Tyr Thr Thr Glu Glu Asp Phe Glu Lys Thr Ile Asp 180 185 190 gcc atc ggc acc ggt gag aac ggc cgc tac ctg ctg gcc gcg acc ttc 924 Ala Ile Gly Thr Gly Glu Asn Gly Arg Tyr Leu Leu Ala Ala Thr Phe 195 200 205 ggc aac gtc cac ggc gtg tac aag ccg ggc aac gtg aag ctg cgt ccg 972 Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro 210 215 220 gag gtc ctg ctc gag ggc cag aag gtg gcc cgc cgc aag ctg ggt ctg 1020 Glu Val Leu Leu Glu Gly Gln Lys Val Ala Arg Arg Lys Leu Gly Leu 225 230 235 240 ggc gac gac gcc ctg ccg ttc gac ttc gtc ttc cac ggt ggc tcc ggc 1068 Gly Asp Asp Ala Leu Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly 245 250 255 tcc gag aag gag aag atc gag gag gcg ctg cgt tac ggc acc atc aag 1116 Ser Glu Lys Glu Lys Ile Glu Glu Ala Leu Arg Tyr Gly Thr Ile Lys 260 265 270 atg aac gtc gac acg gac acc cag tac gcg ttc acc cgt ccg atc gtc 1164 Met Asn Val Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val 275 280 285 acg cac atg ttc cag aac tac gac ggc gtc ctc aag atc gac ggc gag 1212 Thr His Met Phe Gln Asn Tyr Asp Gly Val Leu Lys Ile Asp Gly Glu 290 295 300 gtg ggc aac aag aag gcc tac gac ccg cgt tcc tac ctg aag aag gcc 1260 Val Gly Asn Lys Lys Ala Tyr Asp Pro Arg Ser Tyr Leu Lys Lys Ala 305 310 315 320 gag cag tcg atg gct gag cgc gtc atc gag gcc tgc cag gac ctg cac 1308 Glu Gln Ser Met Ala Glu Arg Val Ile Glu Ala Cys Gln Asp Leu His 325 330 335 tcc gtg ggc aag acc gtc aag tag tcttctccac ccgaggaaag ccccgccctg 1362 Ser Val Gly Lys Thr Val Lys 340 tgcggggctt tctgcgctta gtatcggtgc atggccaaac tgagaatcgg caccctggaa 1422 cgtctccgcc agctcgaggg ctcgttggag tcccgggcgc tgcgcgtgcg gaagcgcttc 1482 ttccggatcg tgcaggtcgc ggtggccgcg ggggtgtcgt tctgggtggc gcagtccctc 1542 atcgggcatc cggtcccgtt cttcgcgccg atcacggtga tcatcatcct ggggctcacc 1602 ggcggcgacc gcatccggcg ggccctggag 1632 <210> 158 <211> 343 <212> PRT <213> Corynebacterium humireducens <400> 158 Met Pro Ile Ala Thr Pro Glu Ile Tyr Asn Glu Met Leu Asp Arg Ala 1 5 10 15 Lys Glu Gly Gly Tyr Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu 20 25 30 Thr Ile Asn Ala Ala Leu Arg Gly Phe Ala Glu Ala Glu Ser Asp Gly 35 40 45 Ile Ile Gln Phe Ser Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ser 50 55 60 Val Lys Asn Lys Val Ala Gly Ala Val Ala Leu Ala Ser Phe Ala His 65 70 75 80 Glu Ala Ala Lys His Tyr Gly Val Asn Val Ala Leu His Thr Asp His 85 90 95 Cys Gln Lys Glu Val Leu Asp Glu Phe Val Arg Pro Leu Leu Ala Ile 100 105 110 Ser Gln Glu Arg Val Asp Ala Gly Glu Leu Pro Leu Phe Gln Ser His 115 120 125 Met Trp Asp Gly Ser Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala 130 135 140 Lys Glu Leu Leu Glu Lys Ser Arg Lys Ala Asn Ile Ile Leu Glu Ile 145 150 155 160 Glu Ile Gly Val Val Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala 165 170 175 Gly Ala Asn Leu Tyr Thr Thr Glu Glu Asp Phe Glu Lys Thr Ile Asp 180 185 190 Ala Ile Gly Thr Gly Glu Asn Gly Arg Tyr Leu Leu Ala Ala Thr Phe 195 200 205 Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro 210 215 220 Glu Val Leu Leu Glu Gly Gln Lys Val Ala Arg Arg Lys Leu Gly Leu 225 230 235 240 Gly Asp Asp Ala Leu Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly 245 250 255 Ser Glu Lys Glu Lys Ile Glu Glu Ala Leu Arg Tyr Gly Thr Ile Lys 260 265 270 Met Asn Val Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val 275 280 285 Thr His Met Phe Gln Asn Tyr Asp Gly Val Leu Lys Ile Asp Gly Glu 290 295 300 Val Gly Asn Lys Lys Ala Tyr Asp Pro Arg Ser Tyr Leu Lys Lys Ala 305 310 315 320 Glu Gln Ser Met Ala Glu Arg Val Ile Glu Ala Cys Gln Asp Leu His 325 330 335 Ser Val Gly Lys Thr Val Lys 340 <210> 159 <211> 1566 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1266) <223> gene 1-phosphofructokinase <400> 159 agaacaccac aaaatccact agttagcaac gatccagaca ggcaaccctt gacaaacgga 60 cacaatcgga caatgatgat gttgtaacac cacattaaat gtgacttaat tcatcatttt 120 cataacgtcc cgcccgggac agccccggag cgccccgatc cccgggtcgc gccccatagg 180 attgatccag ctcaggacct gttcggtcac ccgaccggac accgctccgg gcacgcgtcc 240 cccgccaccc agcggacacg ccgcaacgac acgtttttcc gaacggacag ggagcccgcc 300 gtg att ctc acc ctc acc ccc aac ccc agc atc gac gcg acc atg caa 348 Val Ile Leu Thr Leu Thr Pro Asn Pro Ser Ile Asp Ala Thr Met Gln 1 5 10 15 ctg ggg acg cgg ctg cgc cgc ggg ctc gtc cac cgt ccc tcc tcc atc 396 Leu Gly Thr Arg Leu Arg Arg Gly Leu Val His Arg Pro Ser Ser Ile 20 25 30 tcc cag gtg gcc ggc gga aaa ggc gtc aac gtc acg cac gcc ctg acc 444 Ser Gln Val Ala Gly Gly Lys Gly Val Asn Val Thr His Ala Leu Thr 35 40 45 ctc gcg gac aaa ctc agc ctc gcc ctc ttc ccg gcg gac ggc aac gac 492 Leu Ala Asp Lys Leu Ser Leu Ala Leu Phe Pro Ala Asp Gly Asn Asp 50 55 60 ccc ttc gtc gcc ctg gcc cgc cag tcc ggg atc ccc ttc cac gcc atc 540 Pro Phe Val Ala Leu Ala Arg Gln Ser Gly Ile Pro Phe His Ala Ile 65 70 75 80 ccg gtg tcc gag ggc gtc cgc atc aac acc acc ctc act gaa ccg gac 588 Pro Val Ser Glu Gly Val Arg Ile Asn Thr Thr Leu Thr Glu Pro Asp 85 90 95 ggg acc acc acc aag atc aac ggc ctc ggc gcg cag atc gag ccg ccc 636 Gly Thr Thr Thr Lys Ile Asn Gly Leu Gly Ala Gln Ile Glu Pro Pro 100 105 110 acc cgc cgc atc atc gag gag acc gtc gtc cgc ctc gcc gcg aac gcc 684 Thr Arg Arg Ile Ile Glu Glu Thr Val Val Arg Leu Ala Ala Asn Ala 115 120 125 tcc tgg ctg gtc atg gcc ggt tcc ctg ccg ccc ggg gtc ccc acc ggc 732 Ser Trp Leu Val Met Ala Gly Ser Leu Pro Pro Gly Val Pro Thr Gly 130 135 140 tgg tac acg gaa ctc atc acc acc gtc cgc gcg acg cac ccg gac ctg 780 Trp Tyr Thr Glu Leu Ile Thr Thr Val Arg Ala Thr His Pro Asp Leu 145 150 155 160 ccg gtg gcc gtc gac acg tcc gac gcc gcg atg atc gcc ctc ggc gag 828 Pro Val Ala Val Asp Thr Ser Asp Ala Ala Met Ile Ala Leu Gly Glu 165 170 175 ggc ttc gag gtc gcc gcc ccg acc ctc atc aag ccc aac ggc atg gag 876 Gly Phe Glu Val Ala Ala Pro Thr Leu Ile Lys Pro Asn Gly Met Glu 180 185 190 ctc ggg cag ctc gtc gac cgt gac ggc gag cac ctc gag gcg gcc gcc 924 Leu Gly Gln Leu Val Asp Arg Asp Gly Glu His Leu Glu Ala Ala Ala 195 200 205 gcc cgc ggc gac ttc tcc ccg gtc atc gac gcg gcc cgc gtc gtc gtc 972 Ala Arg Gly Asp Phe Ser Pro Val Ile Asp Ala Ala Arg Val Val Val 210 215 220 gac cgc ggc gtc aac gag gtc ctg gtg acg ctc ggc gtc gcc gga gcc 1020 Asp Arg Gly Val Asn Glu Val Leu Val Thr Leu Gly Val Ala Gly Ala 225 230 235 240 gcc ctc gtc acc gcg cag ggc gtc tgg ctc gcc acc cct ccc ccg gtc 1068 Ala Leu Val Thr Ala Gln Gly Val Trp Leu Ala Thr Pro Pro Pro Val 245 250 255 gac gtc atg tcc acc gtc ggc gcg ggc gac agc tcg ctc gcc ggg tac 1116 Asp Val Met Ser Thr Val Gly Ala Gly Asp Ser Ser Leu Ala Gly Tyr 260 265 270 ctc atg gcc cgc gcg gag ggc ggc agt ccc gcc gac tgc ctg gcc cgc 1164 Leu Met Ala Arg Ala Glu Gly Gly Ser Pro Ala Asp Cys Leu Ala Arg 275 280 285 gcc gtc gcc tac ggc acc gcc gcc acc ggc ctg ccc ggc acc acc att 1212 Ala Val Ala Tyr Gly Thr Ala Ala Thr Gly Leu Pro Gly Thr Thr Ile 290 295 300 cct tcc ccc gca caa ctg aat ctc gag gaa acc cat gtc cgt cat atc 1260 Pro Ser Pro Ala Gln Leu Asn Leu Glu Glu Thr His Val Arg His Ile 305 310 315 320 tcc tga cctggtacac ctggacgccg acctcggcgg cacgaccacc gaggtgatca 1316 Ser ccgccctggc gggcgtcgtc cacgccgccg gccgcgccag ctccccgcaa ctgctcgccg 1376 acgccgccct cgcccgtgag gcccagaacc ccaccggtgt gcccggccgc gtcgccatcc 1436 cccactgccg ttccgccgcc gtcgaggtcc cgaccctggc cttcgcccga ctgtcgcgcc 1496 ccgtcgactt cggcggtccc gacggcgacg ccgagctcgt cttcctcatc gccgcccccg 1556 agggcggcgg 1566 <210> 160 <211> 321 <212> PRT <213> Corynebacterium humireducens <400> 160 Val Ile Leu Thr Leu Thr Pro Asn Pro Ser Ile Asp Ala Thr Met Gln 1 5 10 15 Leu Gly Thr Arg Leu Arg Arg Gly Leu Val His Arg Pro Ser Ser Ile 20 25 30 Ser Gln Val Ala Gly Gly Lys Gly Val Asn Val Thr His Ala Leu Thr 35 40 45 Leu Ala Asp Lys Leu Ser Leu Ala Leu Phe Pro Ala Asp Gly Asn Asp 50 55 60 Pro Phe Val Ala Leu Ala Arg Gln Ser Gly Ile Pro Phe His Ala Ile 65 70 75 80 Pro Val Ser Glu Gly Val Arg Ile Asn Thr Thr Leu Thr Glu Pro Asp 85 90 95 Gly Thr Thr Thr Lys Ile Asn Gly Leu Gly Ala Gln Ile Glu Pro Pro 100 105 110 Thr Arg Arg Ile Ile Glu Glu Thr Val Val Arg Leu Ala Ala Asn Ala 115 120 125 Ser Trp Leu Val Met Ala Gly Ser Leu Pro Pro Gly Val Pro Thr Gly 130 135 140 Trp Tyr Thr Glu Leu Ile Thr Thr Val Arg Ala Thr His Pro Asp Leu 145 150 155 160 Pro Val Ala Val Asp Thr Ser Asp Ala Ala Met Ile Ala Leu Gly Glu 165 170 175 Gly Phe Glu Val Ala Ala Pro Thr Leu Ile Lys Pro Asn Gly Met Glu 180 185 190 Leu Gly Gln Leu Val Asp Arg Asp Gly Glu His Leu Glu Ala Ala Ala 195 200 205 Ala Arg Gly Asp Phe Ser Pro Val Ile Asp Ala Ala Arg Val Val Val 210 215 220 Asp Arg Gly Val Asn Glu Val Leu Val Thr Leu Gly Val Ala Gly Ala 225 230 235 240 Ala Leu Val Thr Ala Gln Gly Val Trp Leu Ala Thr Pro Pro Pro Val 245 250 255 Asp Val Met Ser Thr Val Gly Ala Gly Asp Ser Ser Leu Ala Gly Tyr 260 265 270 Leu Met Ala Arg Ala Glu Gly Gly Ser Pro Ala Asp Cys Leu Ala Arg 275 280 285 Ala Val Ala Tyr Gly Thr Ala Ala Thr Gly Leu Pro Gly Thr Thr Ile 290 295 300 Pro Ser Pro Ala Gln Leu Asn Leu Glu Glu Thr His Val Arg His Ile 305 310 315 320 Ser <210> 161 <211> 1632 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1332) <223> gene 6-phosphofructokinase <400> 161 tggtgtgggc gcccaactcg gcgacggcga tgcggaccgt cgacatcgcc tacatgggtg 60 cggcggccgg cgtgtacaac acggggcgcc agaccggtgc ggtcctcggc gcggccgggg 120 tgggggcggc gatgcaggtc ggtgcggcca cggccgggtt cgtgccgggt ctcgctctgt 180 cgctgctgct ccccgtggcc gtcctcggtc tggggctggt ggcggtcgcc ttcttcgaga 240 ccccgccaca cgccgaacca cagggtgccg aacagcgggc cggggactaa agtggtctcc 300 atg cga ctt gcc acc ctc acc tcc ggc ggc gac tgc ccc ggc ctc aac 348 Met Arg Leu Ala Thr Leu Thr Ser Gly Gly Asp Cys Pro Gly Leu Asn 1 5 10 15 gcc gtc atc cgc ggc atc gtc cgt acc gcc agc tcc gag ttc ggt tcc 396 Ala Val Ile Arg Gly Ile Val Arg Thr Ala Ser Ser Glu Phe Gly Ser 20 25 30 acc gtc gtg gga tac cag gac ggc tgg cag ggc ctg ctg gag gac cgc 444 Thr Val Val Gly Tyr Gln Asp Gly Trp Gln Gly Leu Leu Glu Asp Arg 35 40 45 cgc gtc gac ctc tac gac gac gag cac atc gac cgc atc ctc ctg cgc 492 Arg Val Asp Leu Tyr Asp Asp Glu His Ile Asp Arg Ile Leu Leu Arg 50 55 60 ggt ggc acg atc ctg ggc acc ggt cgt ctg cac ccg gac aag ttc aag 540 Gly Gly Thr Ile Leu Gly Thr Gly Arg Leu His Pro Asp Lys Phe Lys 65 70 75 80 gcg ggg ctc gac cag atc aag cag aac ctg gat gac gcg ggc atc gac 588 Ala Gly Leu Asp Gln Ile Lys Gln Asn Leu Asp Asp Ala Gly Ile Asp 85 90 95 gcc ctc atc ccg atc ggc ggc gag ggc acc ctc aag ggc gcg aag tgg 636 Ala Leu Ile Pro Ile Gly Gly Glu Gly Thr Leu Lys Gly Ala Lys Trp 100 105 110 ctg tcc gac aac ggc atc ccg gtc gtc ggc gtg ccg aag acc atc gac 684 Leu Ser Asp Asn Gly Ile Pro Val Val Gly Val Pro Lys Thr Ile Asp 115 120 125 aac gac gtc tac ggc acc gac tac acc ttc ggc ttc gac acc gcc gtc 732 Asn Asp Val Tyr Gly Thr Asp Tyr Thr Phe Gly Phe Asp Thr Ala Val 130 135 140 gcc gtg gcc acc gac gcc gtc gac cgt ctg cac acc acc gcc gag tcc 780 Ala Val Ala Thr Asp Ala Val Asp Arg Leu His Thr Thr Ala Glu Ser 145 150 155 160 cac aac cgc atc ctc atc gtc gag gtc atg ggc cgt cac gtc ggc tgg 828 His Asn Arg Ile Leu Ile Val Glu Val Met Gly Arg His Val Gly Trp 165 170 175 atc gcg ctg cac gcg ggc atg gcc ggc ggc gcg cac tac acc gtg atc 876 Ile Ala Leu His Ala Gly Met Ala Gly Gly Ala His Tyr Thr Val Ile 180 185 190 ccg gag gtg ccc ttc gac atc tcc gag atc tgc aag aag atg gag cgc 924 Pro Glu Val Pro Phe Asp Ile Ser Glu Ile Cys Lys Lys Met Glu Arg 195 200 205 cgc ttc cag atg ggc gag aag tac ggc atc atc gtc gtc gcc gag ggc 972 Arg Phe Gln Met Gly Glu Lys Tyr Gly Ile Ile Val Val Ala Glu Gly 210 215 220 gcc gcg ccc aag gag ggc acg atg gag ctc aag tcc ggt ggc gtc gac 1020 Ala Ala Pro Lys Glu Gly Thr Met Glu Leu Lys Ser Gly Gly Val Asp 225 230 235 240 cag ttc ggc cac gag atc ttc acc ggc atc ggc cag cag atc gcc gac 1068 Gln Phe Gly His Glu Ile Phe Thr Gly Ile Gly Gln Gln Ile Ala Asp 245 250 255 gag atc cac gcc cgc ctc ggc cat gac gtc cgc acc tcc gtc ctg ggc 1116 Glu Ile His Ala Arg Leu Gly His Asp Val Arg Thr Ser Val Leu Gly 260 265 270 cac atc cag cgc ggt ggc acc ccg acc gcc ttc gac cgc gtc ctg gcc 1164 His Ile Gln Arg Gly Gly Thr Pro Thr Ala Phe Asp Arg Val Leu Ala 275 280 285 acc cgt tac ggc gtc cgc gcg acc cgc tcc gcc cac cgc ggc gag ttc 1212 Thr Arg Tyr Gly Val Arg Ala Thr Arg Ser Ala His Arg Gly Glu Phe 290 295 300 ggc acc tgc gtg gcg ctc aag ggt gag cgg atc gag aac atc acg ctg 1260 Gly Thr Cys Val Ala Leu Lys Gly Glu Arg Ile Glu Asn Ile Thr Leu 305 310 315 320 gag aag gcc gtg ggc acc ctc aag acc gtg ccg atg gac cgc tgg ctc 1308 Glu Lys Ala Val Gly Thr Leu Lys Thr Val Pro Met Asp Arg Trp Leu 325 330 335 acc gcc cag gcc atg ttc ggc tag gtgcgcgacc tccatctgga cacctgccgg 1362 Thr Ala Gln Ala Met Phe Gly 340 ctgctcgtcg tcggcatcaa cccgggtctg gtgtcggagc gcgtcgacgc gcccttcgcc 1422 cgcccgggca accgtttctg gccggcgctc cacgccgccg gcatcacgga gacgcttgtc 1482 gacgcccgcg acggcctcac cgccgcccac cacgcggagc tgctgcggcg gggcctcggc 1542 ttcacgaacg tcgtgccgcg ggccacggcg gtggcctccg agctgacccg cgaggagtac 1602 ctggcgggtg gggcggtgct ggcggagaag 1632 <210> 162 <211> 343 <212> PRT <213> Corynebacterium humireducens <400> 162 Met Arg Leu Ala Thr Leu Thr Ser Gly Gly Asp Cys Pro Gly Leu Asn 1 5 10 15 Ala Val Ile Arg Gly Ile Val Arg Thr Ala Ser Ser Glu Phe Gly Ser 20 25 30 Thr Val Val Gly Tyr Gln Asp Gly Trp Gln Gly Leu Leu Glu Asp Arg 35 40 45 Arg Val Asp Leu Tyr Asp Asp Glu His Ile Asp Arg Ile Leu Leu Arg 50 55 60 Gly Gly Thr Ile Leu Gly Thr Gly Arg Leu His Pro Asp Lys Phe Lys 65 70 75 80 Ala Gly Leu Asp Gln Ile Lys Gln Asn Leu Asp Asp Ala Gly Ile Asp 85 90 95 Ala Leu Ile Pro Ile Gly Gly Glu Gly Thr Leu Lys Gly Ala Lys Trp 100 105 110 Leu Ser Asp Asn Gly Ile Pro Val Val Gly Val Pro Lys Thr Ile Asp 115 120 125 Asn Asp Val Tyr Gly Thr Asp Tyr Thr Phe Gly Phe Asp Thr Ala Val 130 135 140 Ala Val Ala Thr Asp Ala Val Asp Arg Leu His Thr Thr Ala Glu Ser 145 150 155 160 His Asn Arg Ile Leu Ile Val Glu Val Met Gly Arg His Val Gly Trp 165 170 175 Ile Ala Leu His Ala Gly Met Ala Gly Gly Ala His Tyr Thr Val Ile 180 185 190 Pro Glu Val Pro Phe Asp Ile Ser Glu Ile Cys Lys Lys Met Glu Arg 195 200 205 Arg Phe Gln Met Gly Glu Lys Tyr Gly Ile Ile Val Val Ala Glu Gly 210 215 220 Ala Ala Pro Lys Glu Gly Thr Met Glu Leu Lys Ser Gly Gly Val Asp 225 230 235 240 Gln Phe Gly His Glu Ile Phe Thr Gly Ile Gly Gln Gln Ile Ala Asp 245 250 255 Glu Ile His Ala Arg Leu Gly His Asp Val Arg Thr Ser Val Leu Gly 260 265 270 His Ile Gln Arg Gly Gly Thr Pro Thr Ala Phe Asp Arg Val Leu Ala 275 280 285 Thr Arg Tyr Gly Val Arg Ala Thr Arg Ser Ala His Arg Gly Glu Phe 290 295 300 Gly Thr Cys Val Ala Leu Lys Gly Glu Arg Ile Glu Asn Ile Thr Leu 305 310 315 320 Glu Lys Ala Val Gly Thr Leu Lys Thr Val Pro Met Asp Arg Trp Leu 325 330 335 Thr Ala Gln Ala Met Phe Gly 340 <210> 163 <211> 1932 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1632) <223> gene lysA <400> 163 cggacctctc cctgctcacc cacgagcggg agggcgacct catccgcacc ctcggcgagt 60 tcccgaacgt ggtcaaggcc gcggcggaac tgcgtgagcc gcaccgtatc gcccgttatg 120 ccgaggaact ggccggcgtg ttccaccgct tctacgacaa ctgccagatc ctgccgaagg 180 cggacgaaga ggccgccccg atccacaccg cccgcctggc gctggctgcg gccacccgcc 240 agaccctggc caacgcgctc ggcctggtcg gtgtcaccgc cccggagagg atgtaagcgc 300 atg acc tcg gat ttc aac gat ctg ccg gcc cac gtc tgg ccg cgc aac 348 Met Thr Ser Asp Phe Asn Asp Leu Pro Ala His Val Trp Pro Arg Asn 1 5 10 15 gcc gcg cgc cag gag gac ggc gtg gtc acc gtc gcg ggt gtc ccg ctg 396 Ala Ala Arg Gln Glu Asp Gly Val Val Thr Val Ala Gly Val Pro Leu 20 25 30 ccg gag atc gcc gag gag ttc ggt acc ccg gtc ttc gtc gtc gac gag 444 Pro Glu Ile Ala Glu Glu Phe Gly Thr Pro Val Phe Val Val Asp Glu 35 40 45 gac gac ttc cgt tcc cgc tgc cag gac atg gcc cgc gcc ttc ggc ggc 492 Asp Asp Phe Arg Ser Arg Cys Gln Asp Met Ala Arg Ala Phe Gly Gly 50 55 60 ccg gag cac gtc cac tac gcc tcc aag gcg ttc ctc acc cgc acc gtc 540 Pro Glu His Val His Tyr Ala Ser Lys Ala Phe Leu Thr Arg Thr Val 65 70 75 80 gcc cgc tgg gtc gac gag gag ggg ctg tcg ctc gac gtc gcc tcg ctc 588 Ala Arg Trp Val Asp Glu Glu Gly Leu Ser Leu Asp Val Ala Ser Leu 85 90 95 aac gag ctg cgg atc gcg ctg gcc gcc gag ttc ccc gcc gcc cgc atc 636 Asn Glu Leu Arg Ile Ala Leu Ala Ala Glu Phe Pro Ala Ala Arg Ile 100 105 110 acc gcg cac ggc aac aac aag ggc ccg tcc ttc ctg cgg gcg tgc gtc 684 Thr Ala His Gly Asn Asn Lys Gly Pro Ser Phe Leu Arg Ala Cys Val 115 120 125 acc gag ggc gtc ggc cac gtc gtc ctc gac tcc gag cag gag ctc gag 732 Thr Glu Gly Val Gly His Val Val Leu Asp Ser Glu Gln Glu Leu Glu 130 135 140 ctt ctc gac gcc atc gcc gcc ggc gcc ggc gtc atc cag aag gtg atg 780 Leu Leu Asp Ala Ile Ala Ala Gly Ala Gly Val Ile Gln Lys Val Met 145 150 155 160 atc cgc gtc aag ccg ggc atc gag gcg cac acc cac gag gcc atc gcc 828 Ile Arg Val Lys Pro Gly Ile Glu Ala His Thr His Glu Ala Ile Ala 165 170 175 acg tcc cac gag gac cag aag ttc ggc ttc tcc ctg gcc tcc ggc tcg 876 Thr Ser His Glu Asp Gln Lys Phe Gly Phe Ser Leu Ala Ser Gly Ser 180 185 190 gcg ttc cgc gcc gcg gag gcc gcc gtc cgc gcc gag aac ctc gag ctc 924 Ala Phe Arg Ala Ala Glu Ala Ala Val Arg Ala Glu Asn Leu Glu Leu 195 200 205 gtg ggc ctg cac tgc cac gtc ggc tcc cag gtc ttc gac gcc cgg ggc 972 Val Gly Leu His Cys His Val Gly Ser Gln Val Phe Asp Ala Arg Gly 210 215 220 ttc acg atg gcc gcc gac cgc gtg ctg ggc ctc tac tcg cag atc cac 1020 Phe Thr Met Ala Ala Asp Arg Val Leu Gly Leu Tyr Ser Gln Ile His 225 230 235 240 cgc gaa ctc ggc gtg cag ctg ccc gag ctc gat ctc ggc ggc ggc tac 1068 Arg Glu Leu Gly Val Gln Leu Pro Glu Leu Asp Leu Gly Gly Gly Tyr 245 250 255 ggc atc gcc tac acc gag gac gag cag ccc ctc aac gtc gac gag gtc 1116 Gly Ile Ala Tyr Thr Glu Asp Glu Gln Pro Leu Asn Val Asp Glu Val 260 265 270 gcc cac gac ctg ctc acc gcc gtg gcc aag acc gcc gcg gag ctg ggc 1164 Ala His Asp Leu Leu Thr Ala Val Ala Lys Thr Ala Ala Glu Leu Gly 275 280 285 atc gac gcc ccc gcc gtc ctc gtc gag ccg ggc cgc gcc atc gcc ggc 1212 Ile Asp Ala Pro Ala Val Leu Val Glu Pro Gly Arg Ala Ile Ala Gly 290 295 300 ccc ggc acg gtg acg gtc tac gag gtc ggc acc gtc aag gac gtc acc 1260 Pro Gly Thr Val Thr Val Tyr Glu Val Gly Thr Val Lys Asp Val Thr 305 310 315 320 gtc tcc gag gac gag aag cga cgc tac ctc tcc gtc gac ggc ggc atg 1308 Val Ser Glu Asp Glu Lys Arg Arg Tyr Leu Ser Val Asp Gly Gly Met 325 330 335 tcc gac aac atc cgc ccg gcc ctc tac gac gcg ctt tac gac gcc cgc 1356 Ser Asp Asn Ile Arg Pro Ala Leu Tyr Asp Ala Leu Tyr Asp Ala Arg 340 345 350 gtg gtc aac cgt ttc acc gag ggc gag gag atc tcc tcc cgc ctc gtc 1404 Val Val Asn Arg Phe Thr Glu Gly Glu Glu Ile Ser Ser Arg Leu Val 355 360 365 ggt tcc cac tgc gag tcc ggc gac att ctc gtc cgc gac gcg cgc tac 1452 Gly Ser His Cys Glu Ser Gly Asp Ile Leu Val Arg Asp Ala Arg Tyr 370 375 380 ccc gac gac atc gtg gtc ggg gac ctc atg gcc gtc gcc gcg acc ggc 1500 Pro Asp Asp Ile Val Val Gly Asp Leu Met Ala Val Ala Ala Thr Gly 385 390 395 400 gcc tac gcc tac gcc atg tcc tcg cgc tac aac gcc ttc ggc cgt ccg 1548 Ala Tyr Ala Tyr Ala Met Ser Ser Arg Tyr Asn Ala Phe Gly Arg Pro 405 410 415 ccg gtc gtc gcg gtg cgt gcc ggc aag acg aag ctc atg ctg cgc cgc 1596 Pro Val Val Ala Val Arg Ala Gly Lys Thr Lys Leu Met Leu Arg Arg 420 425 430 gag acc gtc gag gac att ctc gcg ctg gag acg tag aaccgacgcc 1642 Glu Thr Val Glu Asp Ile Leu Ala Leu Glu Thr 435 440 cgggcagggc gtcagtggaa gaggcccttg aggctgccgg cgtcctccag cagcgccacg 1702 acgagcgagg tgtggatgga accgagcggc acgcccctgg cgggcgtcgg cgcggtcgac 1762 gggtccgggg tggaggaacg ggtcggcgtc gtggacgact cttcgcggtc gacggtgccg 1822 gaggctcccg tggcgcacat gcgcagctcc gcccggtagg cgtccgcctc cgcctgggct 1882 cccggggtgc gctcccaggc ccgcagccag gcctcgtcgg cggccacgga 1932 <210> 164 <211> 443 <212> PRT <213> Corynebacterium humireducens <400> 164 Met Thr Ser Asp Phe Asn Asp Leu Pro Ala His Val Trp Pro Arg Asn 1 5 10 15 Ala Ala Arg Gln Glu Asp Gly Val Val Thr Val Ala Gly Val Pro Leu 20 25 30 Pro Glu Ile Ala Glu Glu Phe Gly Thr Pro Val Phe Val Val Asp Glu 35 40 45 Asp Asp Phe Arg Ser Arg Cys Gln Asp Met Ala Arg Ala Phe Gly Gly 50 55 60 Pro Glu His Val His Tyr Ala Ser Lys Ala Phe Leu Thr Arg Thr Val 65 70 75 80 Ala Arg Trp Val Asp Glu Glu Gly Leu Ser Leu Asp Val Ala Ser Leu 85 90 95 Asn Glu Leu Arg Ile Ala Leu Ala Ala Glu Phe Pro Ala Ala Arg Ile 100 105 110 Thr Ala His Gly Asn Asn Lys Gly Pro Ser Phe Leu Arg Ala Cys Val 115 120 125 Thr Glu Gly Val Gly His Val Val Leu Asp Ser Glu Gln Glu Leu Glu 130 135 140 Leu Leu Asp Ala Ile Ala Ala Gly Ala Gly Val Ile Gln Lys Val Met 145 150 155 160 Ile Arg Val Lys Pro Gly Ile Glu Ala His Thr His Glu Ala Ile Ala 165 170 175 Thr Ser His Glu Asp Gln Lys Phe Gly Phe Ser Leu Ala Ser Gly Ser 180 185 190 Ala Phe Arg Ala Ala Glu Ala Ala Val Arg Ala Glu Asn Leu Glu Leu 195 200 205 Val Gly Leu His Cys His Val Gly Ser Gln Val Phe Asp Ala Arg Gly 210 215 220 Phe Thr Met Ala Ala Asp Arg Val Leu Gly Leu Tyr Ser Gln Ile His 225 230 235 240 Arg Glu Leu Gly Val Gln Leu Pro Glu Leu Asp Leu Gly Gly Gly Tyr 245 250 255 Gly Ile Ala Tyr Thr Glu Asp Glu Gln Pro Leu Asn Val Asp Glu Val 260 265 270 Ala His Asp Leu Leu Thr Ala Val Ala Lys Thr Ala Ala Glu Leu Gly 275 280 285 Ile Asp Ala Pro Ala Val Leu Val Glu Pro Gly Arg Ala Ile Ala Gly 290 295 300 Pro Gly Thr Val Thr Val Tyr Glu Val Gly Thr Val Lys Asp Val Thr 305 310 315 320 Val Ser Glu Asp Glu Lys Arg Arg Tyr Leu Ser Val Asp Gly Gly Met 325 330 335 Ser Asp Asn Ile Arg Pro Ala Leu Tyr Asp Ala Leu Tyr Asp Ala Arg 340 345 350 Val Val Asn Arg Phe Thr Glu Gly Glu Glu Ile Ser Ser Arg Leu Val 355 360 365 Gly Ser His Cys Glu Ser Gly Asp Ile Leu Val Arg Asp Ala Arg Tyr 370 375 380 Pro Asp Asp Ile Val Val Gly Asp Leu Met Ala Val Ala Ala Thr Gly 385 390 395 400 Ala Tyr Ala Tyr Ala Met Ser Ser Arg Tyr Asn Ala Phe Gly Arg Pro 405 410 415 Pro Val Val Ala Val Arg Ala Gly Lys Thr Lys Leu Met Leu Arg Arg 420 425 430 Glu Thr Val Glu Asp Ile Leu Ala Leu Glu Thr 435 440 <210> 165 <211> 1770 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1470) <223> gene aat <400> 165 cgcctacgcg ctcaccgtcg tcctggccgc cgagaactac ccggccgccc cggtcaccgg 60 tggggtcatc accggtgacg tcgtgaatga cccgtccaag gtcctccacg ccggtaccgc 120 gcgcaacgag gccggcgagc tggtctccgc cggtggccgc gtgctcaaca tcgtgggcgt 180 cggcaagacc ctcgaggagg cgcgtgacga cgcctacgcc accatcgagc agatccagct 240 cgagggttcc ttctaccgcg gcgacatcgc actgccggcc gtggagggac ggatctccct 300 gtg acc ggt cgg cag ctc gtc agc gac cgc ggc cag gtc cag ccc ttc 348 Val Thr Gly Arg Gln Leu Val Ser Asp Arg Gly Gln Val Gln Pro Phe 1 5 10 15 cgc gtc atg cag atg ctc gac atc gtg cac cga cgc gcc cgc gag ggg 396 Arg Val Met Gln Met Leu Asp Ile Val His Arg Arg Ala Arg Glu Gly 20 25 30 aag cac acc atc atg ctg tgc gcc ggg cag ccc tcc acg ggt gcc ccg 444 Lys His Thr Ile Met Leu Cys Ala Gly Gln Pro Ser Thr Gly Ala Pro 35 40 45 gcc ccg gtc ctg acc gcg gcg gag gag tcc ctc cac gcc tcg ccc ctc 492 Ala Pro Val Leu Thr Ala Ala Glu Glu Ser Leu His Ala Ser Pro Leu 50 55 60 ggg tac acc gag gtg gtc ggt gac cgc gac ctc cgg gag atc gtc gcg 540 Gly Tyr Thr Glu Val Val Gly Asp Arg Asp Leu Arg Glu Ile Val Ala 65 70 75 80 gcc tgg cac tcc gac acc tac ggc gtg cac acc agc gcg gac aac gtg 588 Ala Trp His Ser Asp Thr Tyr Gly Val His Thr Ser Ala Asp Asn Val 85 90 95 gtg atc acc acc ggc tcc tcc ggt ggc ttc gtc gcg tcc ttc ctg gcc 636 Val Ile Thr Thr Gly Ser Ser Gly Gly Phe Val Ala Ser Phe Leu Ala 100 105 110 gtg ctc gac cac ggc gac gcc gtc gcc ctg tcc tgc ccc ggt tac ccg 684 Val Leu Asp His Gly Asp Ala Val Ala Leu Ser Cys Pro Gly Tyr Pro 115 120 125 gcg tac cgc aac atc ctg gag tcc ctc ggg gcg cgg gtg gtc aac ctc 732 Ala Tyr Arg Asn Ile Leu Glu Ser Leu Gly Ala Arg Val Val Asn Leu 130 135 140 gtg tgc gac gag tcg acc cgc ttc cag ccg acg gcc gcg atg ctc gag 780 Val Cys Asp Glu Ser Thr Arg Phe Gln Pro Thr Ala Ala Met Leu Glu 145 150 155 160 gca ctg ccg gag gct gac agg ccg cgg gcg gtg atc gtc acc tcc ccg 828 Ala Leu Pro Glu Ala Asp Arg Pro Arg Ala Val Ile Val Thr Ser Pro 165 170 175 ggc aac ccc tcc ggc acg acc atc gac ccc gcg gag ctc gag cgc atc 876 Gly Asn Pro Ser Gly Thr Thr Ile Asp Pro Ala Glu Leu Glu Arg Ile 180 185 190 gcc cgc tgg tgc gag gac aac gac gcc gtg ctc atc tcc gac gag gac 924 Ala Arg Trp Cys Glu Asp Asn Asp Ala Val Leu Ile Ser Asp Glu Asp 195 200 205 tac cac ggc atg tcc ttc ggc cgc ccc ctg gcc acc gca cgc cag ttc 972 Tyr His Gly Met Ser Phe Gly Arg Pro Leu Ala Thr Ala Arg Gln Phe 210 215 220 tcc gac cgg gcg atc gtc gtg ggc acg ctg tcg aag tac tac tcc atg 1020 Ser Asp Arg Ala Ile Val Val Gly Thr Leu Ser Lys Tyr Tyr Ser Met 225 230 235 240 acc ggc tgg cgc gtc ggc tgg ctc atc ctc ccc gac gag ctg gtc acc 1068 Thr Gly Trp Arg Val Gly Trp Leu Ile Leu Pro Asp Glu Leu Val Thr 245 250 255 ccc gtg gag aac ctg cag gcc tcg ctg gcg ctc tgc ccg ccg gcg atc 1116 Pro Val Glu Asn Leu Gln Ala Ser Leu Ala Leu Cys Pro Pro Ala Ile 260 265 270 tcg cag gtc gcc ggg cgt gcc gcc ttc acc ccg gag gcg cgc gcc gaa 1164 Ser Gln Val Ala Gly Arg Ala Ala Phe Thr Pro Glu Ala Arg Ala Glu 275 280 285 ctc gac tcc cac gtc gag cgc tac cgc gcc gcc cgc gcg gtg ctg ctc 1212 Leu Asp Ser His Val Glu Arg Tyr Arg Ala Ala Arg Ala Val Leu Leu 290 295 300 gag gag ctg ccg gcg gcc ggt ttc acc acc atg gcg gaa ccc gac ggc 1260 Glu Glu Leu Pro Ala Ala Gly Phe Thr Thr Met Ala Glu Pro Asp Gly 305 310 315 320 ggg ctc tac ctg tgg ctc gac gtc tcc cac gtc acc gac gac tcc gag 1308 Gly Leu Tyr Leu Trp Leu Asp Val Ser His Val Thr Asp Asp Ser Glu 325 330 335 gcc tgg tgc cac gag ctc gtc gag gag atc ggc gtg gcc ttc gcg ccg 1356 Ala Trp Cys His Glu Leu Val Glu Glu Ile Gly Val Ala Phe Ala Pro 340 345 350 ggc gtc gac ttc gac ccg atc aac ggt cac aag tgg gtc cgc ctc acg 1404 Gly Val Asp Phe Asp Pro Ile Asn Gly His Lys Trp Val Arg Leu Thr 355 360 365 ctg tgc gcc ccg gag gag gag acc cgc gag gcg atc cgg cgc ctc ggc 1452 Leu Cys Ala Pro Glu Glu Glu Thr Arg Glu Ala Ile Arg Arg Leu Gly 370 375 380 gag tac gtg cgt cgc tga ccgcgcggcg acctgcggcg cgggggaaac 1500 Glu Tyr Val Arg Arg 385 ggcccggatc cggggactcc cgggccaccc ccgccgctgg tcatgcgata atcgggggcg 1560 tggctgagaa gaagaagatc gcgaacgtcc tgtccaaccg ctacgcctcc gcggagctga 1620 gcaacctgtg gagcccggag tacaagatca tcctggagcg tcagctctgg atcgccgtga 1680 tgaaggcgca gaaggatctg ggtgtcgaca tcccggctga ggccatcgac gcctacgagg 1740 ccgtcatcga ccgggtcgac ctcgagtcga 1770 <210> 166 <211> 389 <212> PRT <213> Corynebacterium humireducens <400> 166 Val Thr Gly Arg Gln Leu Val Ser Asp Arg Gly Gln Val Gln Pro Phe 1 5 10 15 Arg Val Met Gln Met Leu Asp Ile Val His Arg Arg Ala Arg Glu Gly 20 25 30 Lys His Thr Ile Met Leu Cys Ala Gly Gln Pro Ser Thr Gly Ala Pro 35 40 45 Ala Pro Val Leu Thr Ala Ala Glu Glu Ser Leu His Ala Ser Pro Leu 50 55 60 Gly Tyr Thr Glu Val Val Gly Asp Arg Asp Leu Arg Glu Ile Val Ala 65 70 75 80 Ala Trp His Ser Asp Thr Tyr Gly Val His Thr Ser Ala Asp Asn Val 85 90 95 Val Ile Thr Thr Gly Ser Ser Gly Gly Phe Val Ala Ser Phe Leu Ala 100 105 110 Val Leu Asp His Gly Asp Ala Val Ala Leu Ser Cys Pro Gly Tyr Pro 115 120 125 Ala Tyr Arg Asn Ile Leu Glu Ser Leu Gly Ala Arg Val Val Asn Leu 130 135 140 Val Cys Asp Glu Ser Thr Arg Phe Gln Pro Thr Ala Ala Met Leu Glu 145 150 155 160 Ala Leu Pro Glu Ala Asp Arg Pro Arg Ala Val Ile Val Thr Ser Pro 165 170 175 Gly Asn Pro Ser Gly Thr Thr Ile Asp Pro Ala Glu Leu Glu Arg Ile 180 185 190 Ala Arg Trp Cys Glu Asp Asn Asp Ala Val Leu Ile Ser Asp Glu Asp 195 200 205 Tyr His Gly Met Ser Phe Gly Arg Pro Leu Ala Thr Ala Arg Gln Phe 210 215 220 Ser Asp Arg Ala Ile Val Val Gly Thr Leu Ser Lys Tyr Tyr Ser Met 225 230 235 240 Thr Gly Trp Arg Val Gly Trp Leu Ile Leu Pro Asp Glu Leu Val Thr 245 250 255 Pro Val Glu Asn Leu Gln Ala Ser Leu Ala Leu Cys Pro Pro Ala Ile 260 265 270 Ser Gln Val Ala Gly Arg Ala Ala Phe Thr Pro Glu Ala Arg Ala Glu 275 280 285 Leu Asp Ser His Val Glu Arg Tyr Arg Ala Ala Arg Ala Val Leu Leu 290 295 300 Glu Glu Leu Pro Ala Ala Gly Phe Thr Thr Met Ala Glu Pro Asp Gly 305 310 315 320 Gly Leu Tyr Leu Trp Leu Asp Val Ser His Val Thr Asp Asp Ser Glu 325 330 335 Ala Trp Cys His Glu Leu Val Glu Glu Ile Gly Val Ala Phe Ala Pro 340 345 350 Gly Val Asp Phe Asp Pro Ile Asn Gly His Lys Trp Val Arg Leu Thr 355 360 365 Leu Cys Ala Pro Glu Glu Glu Thr Arg Glu Ala Ile Arg Arg Leu Gly 370 375 380 Glu Tyr Val Arg Arg 385 <210> 167 <211> 1281 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(981) <223> gene lysE <400> 167 gaaggcgatc acctggatgc cgtagcccac cgccgccggg tcgaggcgcg tgtggaagcc 60 ggtgatcacg cccccgttct ccagcgcccg cagccgccgc aggcaggtgc ttgacgacgc 120 ccccgtatga tgcgccagtt ctgcggcggt gatgcgtgca ttgttctgga tcagtgacag 180 aatcatgcgg tcaatgtcgt ccatacgccg gattctgtca cacgtgcagt cccggcgtcc 240 cccggtgaat gaaaagttca ggagtatcgg ccgcggcgcc gggcgggcac gataccgggc 300 atg gac atc acg gac ctc ctg agc ttc tgg gcg gtc agc ctc ctg ctg 348 Met Asp Ile Thr Asp Leu Leu Ser Phe Trp Ala Val Ser Leu Leu Leu 1 5 10 15 atc gtc acc ccg ggc ccc gac tgg gcg ttc gtg ctc ggg cac gct ttc 396 Ile Val Thr Pro Gly Pro Asp Trp Ala Phe Val Leu Gly His Ala Phe 20 25 30 cga cgc cgc ccc ctc gcc ccg ccc ctt ctc ggc atc ggc ctg ggc tac 444 Arg Arg Arg Pro Leu Ala Pro Pro Leu Leu Gly Ile Gly Leu Gly Tyr 35 40 45 ctc ggc ctg acc acc gtg gtg gcc ggc ggc ctc ggg gcg ctc gtg gcc 492 Leu Gly Leu Thr Thr Val Val Ala Gly Gly Leu Gly Ala Leu Val Ala 50 55 60 cgt cac ccc gcg ctg ctc acg ctc gtc acc gtc gcc ggt gtc ctg gtg 540 Arg His Pro Ala Leu Leu Thr Leu Val Thr Val Ala Gly Val Leu Val 65 70 75 80 ctc ctg cgt ctc ggc tgg cag atg atg cgc gcg gcg ctg gcc ggg ccc 588 Leu Leu Arg Leu Gly Trp Gln Met Met Arg Ala Ala Leu Ala Gly Pro 85 90 95 gtc acg gtg gag gtg gac acc gcg gac ccg gct gtc ccg gag ggg ccg 636 Val Thr Val Glu Val Asp Thr Ala Asp Pro Ala Val Pro Glu Gly Pro 100 105 110 gcc ggc ggg gga gtg gcg acg ctg acg cgc ccg cag gtg ggc gtc gag 684 Ala Gly Gly Gly Val Ala Thr Leu Thr Arg Pro Gln Val Gly Val Glu 115 120 125 aag cac gcg gtg gtc acc ggg gcc gcg gtg agt ggc ctc aac ccc aag 732 Lys His Ala Val Val Thr Gly Ala Ala Val Ser Gly Leu Asn Pro Lys 130 135 140 ggg ctc atg ctg ttc atc gcg ctg ctg ccc cag ttc gtg ggc gcg gag 780 Gly Leu Met Leu Phe Ile Ala Leu Leu Pro Gln Phe Val Gly Ala Glu 145 150 155 160 gcg acg tgg ccg gtg tgg acg cag atg ttc gtc ctc gga ctg gtg ttc 828 Ala Thr Trp Pro Val Trp Thr Gln Met Phe Val Leu Gly Leu Val Phe 165 170 175 atc gcc tcg gcg acc gtg ttc tac gcg ctg ctc ggc gtt ttc gcc ggg 876 Ile Ala Ser Ala Thr Val Phe Tyr Ala Leu Leu Gly Val Phe Ala Gly 180 185 190 cgg gtg ctg gcg ggc tcg gag cgg gcc tcc cgg atc ctc acc ggc gtg 924 Arg Val Leu Ala Gly Ser Glu Arg Ala Ser Arg Ile Leu Thr Gly Val 195 200 205 gcc ggc ggg gcg atg atg tgt gtc gcg gca ggg atg ctc gcg cag cac 972 Ala Gly Gly Ala Met Met Cys Val Ala Ala Gly Met Leu Ala Gln His 210 215 220 ctc ctc tga tgggggtagt tgagtagggg cattcatcgg gtgcgagttc 1021 Leu Leu 225 cgcgcctccc gatgaatgcc cctgctcgga taccctccta ctccacgtgc gccggcgtcg 1081 acggcgacac cagcggcatc ggcgcgtact cggcacgcag ctcgcgcagc ttccgggcgt 1141 gctcggagag gcggacgtcc tcggtggtac gggggacgaa ctggcgggcg ggcagggcgt 1201 tgccgtcggc gtccgtcgcc aggtaggtga cggtggcgtg gatggcggtc tgcaggttct 1261 cgcggccccc gcgcgggttg 1281 <210> 168 <211> 226 <212> PRT <213> Corynebacterium humireducens <400> 168 Met Asp Ile Thr Asp Leu Leu Ser Phe Trp Ala Val Ser Leu Leu Leu 1 5 10 15 Ile Val Thr Pro Gly Pro Asp Trp Ala Phe Val Leu Gly His Ala Phe 20 25 30 Arg Arg Arg Pro Leu Ala Pro Pro Leu Leu Gly Ile Gly Leu Gly Tyr 35 40 45 Leu Gly Leu Thr Thr Val Val Ala Gly Gly Leu Gly Ala Leu Val Ala 50 55 60 Arg His Pro Ala Leu Leu Thr Leu Val Thr Val Ala Gly Val Leu Val 65 70 75 80 Leu Leu Arg Leu Gly Trp Gln Met Met Arg Ala Ala Leu Ala Gly Pro 85 90 95 Val Thr Val Glu Val Asp Thr Ala Asp Pro Ala Val Pro Glu Gly Pro 100 105 110 Ala Gly Gly Gly Val Ala Thr Leu Thr Arg Pro Gln Val Gly Val Glu 115 120 125 Lys His Ala Val Val Thr Gly Ala Ala Val Ser Gly Leu Asn Pro Lys 130 135 140 Gly Leu Met Leu Phe Ile Ala Leu Leu Pro Gln Phe Val Gly Ala Glu 145 150 155 160 Ala Thr Trp Pro Val Trp Thr Gln Met Phe Val Leu Gly Leu Val Phe 165 170 175 Ile Ala Ser Ala Thr Val Phe Tyr Ala Leu Leu Gly Val Phe Ala Gly 180 185 190 Arg Val Leu Ala Gly Ser Glu Arg Ala Ser Arg Ile Leu Thr Gly Val 195 200 205 Ala Gly Gly Ala Met Met Cys Val Ala Ala Gly Met Leu Ala Gln His 210 215 220 Leu Leu 225 <210> 169 <211> 1347 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1047) <223> gene dapB <400> 169 ccggtgccga aggagtcgag ccaggtgcgc agggagttgc ggttgactcc gagatcggag 60 gcgatcgcgt tgatcgttgc tccgggagtc gactcgtaca acgccactgc gtcacgcttg 120 aactgctcgg tgtaggtctt gcgtggcatg gtggaaaggt acctcacttc cccagcgaaa 180 tgctggtttc agcgtgtcca ccatcaaggg gtcaggtccg ctggtgatgt tgatgcgcat 240 gcaacgattc tatgcgccta gactggggca ctgaatcttt caggaactca aggaggaacc 300 atg gcg atc aag gtg gga gtg ctc ggc gcg aag ggc cgt gtc ggc tcg 348 Met Ala Ile Lys Val Gly Val Leu Gly Ala Lys Gly Arg Val Gly Ser 1 5 10 15 gcg gtg tgc gag ggc gtc aac ggg gcg gag ggc ctc gag ctc gtc gcg 396 Ala Val Cys Glu Gly Val Asn Gly Ala Glu Gly Leu Glu Leu Val Ala 20 25 30 cag gtc gat cag gac gat gac ctg cag ctg ctt ctc gac gcc ggg gcg 444 Gln Val Asp Gln Asp Asp Asp Leu Gln Leu Leu Leu Asp Ala Gly Ala 35 40 45 gag gtc gtc gtc gac ttc acc act ccg ggt tcc gtg atg ggc aac ctg 492 Glu Val Val Val Asp Phe Thr Thr Pro Gly Ser Val Met Gly Asn Leu 50 55 60 gag ttc tgc atc gcc aac ggc atc cac gcg gtg gtc ggc acc acc ggc 540 Glu Phe Cys Ile Ala Asn Gly Ile His Ala Val Val Gly Thr Thr Gly 65 70 75 80 ttc gac gag gag cgc ctc gcg cag gtc cgt gag tgg tgt gcc gcc gag 588 Phe Asp Glu Glu Arg Leu Ala Gln Val Arg Glu Trp Cys Ala Ala Glu 85 90 95 ggg gcg ggc aac gtc ctc atc gcc ccg aac ttc gcg atc tct gca gtg 636 Gly Ala Gly Asn Val Leu Ile Ala Pro Asn Phe Ala Ile Ser Ala Val 100 105 110 ctg gcc atg gag ttc gcg cgg cag gcc gcc cgc ttc ttc gac tcc gcc 684 Leu Ala Met Glu Phe Ala Arg Gln Ala Ala Arg Phe Phe Asp Ser Ala 115 120 125 gag gtc gtc gag tac cac cac ccc tac aag ctg gac gcc ccc tcc ggc 732 Glu Val Val Glu Tyr His His Pro Tyr Lys Leu Asp Ala Pro Ser Gly 130 135 140 acc gcg atc cac acc gcg cag ggc atc gcc gcc gcc cgc cgg gag gcc 780 Thr Ala Ile His Thr Ala Gln Gly Ile Ala Ala Ala Arg Arg Glu Ala 145 150 155 160 ggc ctc ggc ccc atc ccg gat gcc acc gag cag acc ctc gag ggt gcc 828 Gly Leu Gly Pro Ile Pro Asp Ala Thr Glu Gln Thr Leu Glu Gly Ala 165 170 175 cgc ggc gcc gac gtc gac ggc gtg cgt gtc cac ggc gtg cgc atg acc 876 Arg Gly Ala Asp Val Asp Gly Val Arg Val His Gly Val Arg Met Thr 180 185 190 ggc atg gtc gcc cac gag gac atc atc ttc ggt gcc cag ggc cag tcc 924 Gly Met Val Ala His Glu Asp Ile Ile Phe Gly Ala Gln Gly Gln Ser 195 200 205 ctg acc atc cgc cag gac tcc tac gac cgc acc tcc ttc gtg ccg ggc 972 Leu Thr Ile Arg Gln Asp Ser Tyr Asp Arg Thr Ser Phe Val Pro Gly 210 215 220 gtg ctc ctc ggt gtc cgt gag atc gcg aac cac gac ggc ctg acc gtg 1020 Val Leu Leu Gly Val Arg Glu Ile Ala Asn His Asp Gly Leu Thr Val 225 230 235 240 ggg ctc gac agc tac ctg gga ctc tag atggccaagc ccgtcgacct 1067 Gly Leu Asp Ser Tyr Leu Gly Leu 245 ggacgtccag ctcatcgcgg ccacgcagtt caccccgccc gccggggtcg actgggaggc 1127 cgacgacacc gccaccggcg ccgaggcgct cgtcgagttc gcgggccgcg cctgctacga 1187 gaccttcgac aaacccaacc cgcgcaccgc ggccaacgac gcctacctcc gccacatcat 1247 ggaggtcgga cacaccgcac tgctcgagca cgccaccgcg acgctctaca tccgcggcat 1307 ctcccgctcc gccacccacg agctggtccg ccaccgcaac 1347 <210> 170 <211> 248 <212> PRT <213> Corynebacterium humireducens <400> 170 Met Ala Ile Lys Val Gly Val Leu Gly Ala Lys Gly Arg Val Gly Ser 1 5 10 15 Ala Val Cys Glu Gly Val Asn Gly Ala Glu Gly Leu Glu Leu Val Ala 20 25 30 Gln Val Asp Gln Asp Asp Asp Leu Gln Leu Leu Leu Asp Ala Gly Ala 35 40 45 Glu Val Val Val Asp Phe Thr Thr Pro Gly Ser Val Met Gly Asn Leu 50 55 60 Glu Phe Cys Ile Ala Asn Gly Ile His Ala Val Val Gly Thr Thr Gly 65 70 75 80 Phe Asp Glu Glu Arg Leu Ala Gln Val Arg Glu Trp Cys Ala Ala Glu 85 90 95 Gly Ala Gly Asn Val Leu Ile Ala Pro Asn Phe Ala Ile Ser Ala Val 100 105 110 Leu Ala Met Glu Phe Ala Arg Gln Ala Ala Arg Phe Phe Asp Ser Ala 115 120 125 Glu Val Val Glu Tyr His His Pro Tyr Lys Leu Asp Ala Pro Ser Gly 130 135 140 Thr Ala Ile His Thr Ala Gln Gly Ile Ala Ala Ala Arg Arg Glu Ala 145 150 155 160 Gly Leu Gly Pro Ile Pro Asp Ala Thr Glu Gln Thr Leu Glu Gly Ala 165 170 175 Arg Gly Ala Asp Val Asp Gly Val Arg Val His Gly Val Arg Met Thr 180 185 190 Gly Met Val Ala His Glu Asp Ile Ile Phe Gly Ala Gln Gly Gln Ser 195 200 205 Leu Thr Ile Arg Gln Asp Ser Tyr Asp Arg Thr Ser Phe Val Pro Gly 210 215 220 Val Leu Leu Gly Val Arg Glu Ile Ala Asn His Asp Gly Leu Thr Val 225 230 235 240 Gly Leu Asp Ser Tyr Leu Gly Leu 245 <210> 171 <211> 2145 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1845) <223> gene glucose-6-phosphate dehydrogenase <400> 171 cgaccctcta cgtcaccgag ctggccggcc cggagaccgt caacaccatg ccggagccga 60 ccatcgacgc cgtgctcgag cagggtggcc tccacggcga caccctgagc ggcaccgcgg 120 acgacgcgca ggccgtcttc gagcagctgg tgcaggtcgg catcgacatg gaggacgtct 180 tcgcggtgct ggagcgcgag ggcgtcgaga agttcgtgtc ctcctggtcc gagctgctcg 240 actccatgga gtcccgactg cgctagcagt cccctccccg acaggaaagg acctgagcgc 300 atg ccc ctc ccc gtg acc ggc acc gac gcc tgg atc aac ccg ctg cgg 348 Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg 1 5 10 15 aac acc gtc gac aag cgg ctg ccc cgc atc gcc ggc ccc tcc ggc atg 396 Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met 20 25 30 gtg atc ttc ggc gtg acc ggt gac ctg gcc cgc aag aag ctg ctc ccg 444 Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro 35 40 45 gcc atc tac gac ctg gcc aac cgt ggc ctc ctg ccg gcc ggt ttc acc 492 Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr 50 55 60 ctc atc ggt ttc ggc cgt cgt ccc tgg agc aaa ccc gag ttc gag gag 540 Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu 65 70 75 80 tac gtg cgg gag gcc gtc gag agc ggc gcc cgc acc gag ttc cgc gaa 588 Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu 85 90 95 cac gtg tgg gag cgg ctg gcc gag ggc atg cac ttc gtc gag ggc aac 636 His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn 100 105 110 ttc gac gac gag ggc ttc gac cgc ctc gcc gcc acc ctc gcc gag atg 684 Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met 115 120 125 gac acc tcc cgc ggc acg gcg ggc aac tgg gcc ttc tac ctg tcg gtc 732 Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val 130 135 140 ccg ccg gac ttc ttc acc gcc gtc tgc cac cag ctg gag cgc tcc ggc 780 Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly 145 150 155 160 ctg gcg gag gcc ggt gag gag tcg tgg cgt cgc gtg atc atc gag aag 828 Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys 165 170 175 ccc ttc ggc cac gac cag gac acg gcc cgg gag ctc aac cgg gtg gtc 876 Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val 180 185 190 aac gcc gtc ttc ccg gag gag tcg gtg ttc cgc atc gac cac tac ctg 924 Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu 195 200 205 ggc aag gag acg gtg cag aac atc ctg gcg ctg cgc ttc gcc aac cag 972 Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln 210 215 220 ctc ttc gac ccg atc tgg aac gcg cac tac gtc gac cac gtg cag atc 1020 Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile 225 230 235 240 acc atg gcc gag gac atc ggt ctc ggt ggt cgg gcc ggc tac tac gac 1068 Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp 245 250 255 ggc atc ggc gcc gcc cgc gac gtc atg cag aac cac ctc ctc cag ctc 1116 Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu 260 265 270 ctg gcg ctg gtg gcc atg gag gag ccc atc gcc ttc gac ccg gct gag 1164 Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu 275 280 285 ctg cag gcg gag aag gtc aag gtc ctg cgc gcg acc aac ccc gtc ttc 1212 Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe 290 295 300 ccg ctg aac aag acc acc gcc cgt ggc cag tac gcg gcg ggc tgg cag 1260 Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln 305 310 315 320 ggc tcg cac aag gtg cgt ggc ctg cgt gag gag gag ggt ttc gac ccg 1308 Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro 325 330 335 gac tcc acc acg gag acc tac gcc gcc tgc acc ctg gag atc aac tcc 1356 Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser 340 345 350 cgc cgc tgg gcg ggc gtg ccc ttc tac ctg cgg acc ggc aag cgc ctg 1404 Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu 355 360 365 ggg cgc cgc gtc acg gag atc gcc ctc gtg ttc aag gag gcg ccg cac 1452 Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His 370 375 380 cag ccc ttc ctc gag ggg cag acg tcc tcg ctg ggg cag aac gcg atc 1500 Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile 385 390 395 400 gtc atc cgc gtc cag ccc gac gag ggc gtg ctc atg cgc ttc ggc tcc 1548 Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser 405 410 415 aag gtg ccc ggc tcc gcg atg gag gtc cgt gac gtc aac atg gac ttc 1596 Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe 420 425 430 tcc tac gcg gag gcc ttc acc gag gcg tcc ccg gag gcc tat gag cgg 1644 Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg 435 440 445 ctc atc ctc gat gcg ctt ctc gac gag tcc tcg ctc ttc ccc acc aac 1692 Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn 450 455 460 gag gag gtc gag ctc tcc tgg gcg atc ctg gac ccg atc ctc gag ttc 1740 Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe 465 470 475 480 tgg gct gag aag ggc cag ccc gag gag tac gag tcc ggc acc tgg ggg 1788 Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly 485 490 495 ccc gcc tcc gcc gac cgc atg ctg gaa cgg acc ggc cgc gcc tgg cgc 1836 Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg 500 505 510 cgc ccc tga cctggaaggg acccgacttg aagatcgaac tgccggacac 1885 Arg Pro cgacacccgg aacatcgccg ccagcctgct cgaggcccgg gagcacaact cccagaccac 1945 cgggcgggtg ctcaccctca tcgtcgtcgc cgcgctcgac gacgacgtcg accacatcat 2005 ctccgtcacc cgcgacgcct cccacgagca ccccgcccgt gtgctggtgc tgctcaccgg 2065 ccgggaggac gctgactcgc gtctcgacgc cgaggtgcgc gtcggcggtc acgccggcgc 2125 ctcggagatg gtcatcatgc 2145 <210> 172 <211> 514 <212> PRT <213> Corynebacterium humireducens <400> 172 Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg 1 5 10 15 Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met 20 25 30 Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro 35 40 45 Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr 50 55 60 Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu 65 70 75 80 Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu 85 90 95 His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn 100 105 110 Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met 115 120 125 Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val 130 135 140 Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly 145 150 155 160 Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys 165 170 175 Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val 180 185 190 Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu 195 200 205 Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln 210 215 220 Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile 225 230 235 240 Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp 245 250 255 Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu 260 265 270 Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu 275 280 285 Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe 290 295 300 Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln 305 310 315 320 Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro 325 330 335 Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser 340 345 350 Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu 355 360 365 Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His 370 375 380 Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile 385 390 395 400 Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser 405 410 415 Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe 420 425 430 Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg 435 440 445 Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn 450 455 460 Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe 465 470 475 480 Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly 485 490 495 Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg 500 505 510 Arg Pro <210> 173 <211> 2055 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1755) <223> gene gnd <400> 173 aaggctgccc acggactcgg ccagcgcgca gaacacgccc ccgttgacca ggccgacggg 60 ctgccggtgc tggtccccca cctcgaggcg ggcgcggacg cggccgtcgg cgatctgcgt 120 gtagacgatc ccgaggtggg cgtcgaggcc gacgttgcgg tcgttgagct gcgcgagctg 180 cgcggcgtcg agggccttgc ctgtcccggc gaggcggaag aggtcggtga aactgtaggt 240 ctcggtcatg agtgccgact ctactgaccc ttcgaaaatg gtgtgtctaa actctgtgcc 300 atg acc tcc cct gag aat ctc gcc cag atc ggc gtc gtc gga ctc gcc 348 Met Thr Ser Pro Glu Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala 1 5 10 15 gtc atg ggt tcc aac ctg gcc cgc aac ttc gcc cgc aac ggc cac acc 396 Val Met Gly Ser Asn Leu Ala Arg Asn Phe Ala Arg Asn Gly His Thr 20 25 30 gtc gcc gtg tac aac cgt tcc ccc gag aag acc cgc gac ctc atc gcc 444 Val Ala Val Tyr Asn Arg Ser Pro Glu Lys Thr Arg Asp Leu Ile Ala 35 40 45 gag cac ggt gac gag ggc acc ttc ctc gcc tcc gag acc gtc gag gac 492 Glu His Gly Asp Glu Gly Thr Phe Leu Ala Ser Glu Thr Val Glu Asp 50 55 60 ttc gtc gcc tcc ctc gag cgc ccg cgt aag gcg atc atc atg gtc cag 540 Phe Val Ala Ser Leu Glu Arg Pro Arg Lys Ala Ile Ile Met Val Gln 65 70 75 80 gcc ggc gcg gcc acc gac gcg gtg atc aac cag ctg gcc gac gcc atg 588 Ala Gly Ala Ala Thr Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met 85 90 95 gag gac ggc gac atc atc atc gac ggc ggc aac gcc ctc tac agc gac 636 Glu Asp Gly Asp Ile Ile Ile Asp Gly Gly Asn Ala Leu Tyr Ser Asp 100 105 110 acc atc cgc cgg gag cgg gag atg gcc gag cgc aac cgt cac ttc gtc 684 Thr Ile Arg Arg Glu Arg Glu Met Ala Glu Arg Asn Arg His Phe Val 115 120 125 ggc gcc ggc atc tcc ggc ggc gag gag ggg gcc ctc aac ggc ccg tcg 732 Gly Ala Gly Ile Ser Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser 130 135 140 atc atg ccg ggt ggc ccg aag gag tcc tgg gag acc ctc ggc ccg ctg 780 Ile Met Pro Gly Gly Pro Lys Glu Ser Trp Glu Thr Leu Gly Pro Leu 145 150 155 160 ctg gag tcc gtc gct gcc gtc gtc gac ggc acc ccg tgt gtc acg cac 828 Leu Glu Ser Val Ala Ala Val Val Asp Gly Thr Pro Cys Val Thr His 165 170 175 atc ggc ccg gac ggt gcg ggc cac ttc gtc aag atg gtc cac aac ggc 876 Ile Gly Pro Asp Gly Ala Gly His Phe Val Lys Met Val His Asn Gly 180 185 190 atc gag tac gcc gac atg cag gtc atc ggc gag gcg tac cag ctg ctg 924 Ile Glu Tyr Ala Asp Met Gln Val Ile Gly Glu Ala Tyr Gln Leu Leu 195 200 205 cgc tac ggc gcg ggc atg agc ccg gcc gag atc tcc gag gtc ttc cgc 972 Arg Tyr Gly Ala Gly Met Ser Pro Ala Glu Ile Ser Glu Val Phe Arg 210 215 220 gag tgg aac tcc ggc gac ctc gac tcc tac ctc atc gag atc acc gcc 1020 Glu Trp Asn Ser Gly Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala 225 230 235 240 gag gtc ctc gcg cag gtc gac gcc gag acg ggg aag ccg ctc atc gac 1068 Glu Val Leu Ala Gln Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp 245 250 255 gtc atc gtc gac gcc gcc ggc cag aag ggc acc ggc cgc tgg acc gtc 1116 Val Ile Val Asp Ala Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val 260 265 270 aag gcc gcc ctc gac ctg ggc atc ccg gtc acc ggc atc ggc gag gcc 1164 Lys Ala Ala Leu Asp Leu Gly Ile Pro Val Thr Gly Ile Gly Glu Ala 275 280 285 gtc ttc gcc cgc gcc ctg tcc tcc tcc ctc gag cag cgt gcc gcc gcg 1212 Val Phe Ala Arg Ala Leu Ser Ser Ser Leu Glu Gln Arg Ala Ala Ala 290 295 300 cag ggc gtg ctg ccc gcc ggt gag ctc gcc acc ttc gag tcc ctg ggt 1260 Gln Gly Val Leu Pro Ala Gly Glu Leu Ala Thr Phe Glu Ser Leu Gly 305 310 315 320 ctg gac cgc gac gcc ttc atc gag gac gtc cgc cgc gcc ctc tac gcc 1308 Leu Asp Arg Asp Ala Phe Ile Glu Asp Val Arg Arg Ala Leu Tyr Ala 325 330 335 tcc aag ctg gtc gcc tac gcg cag ggc ttc gac gag atc cgg gcc ggc 1356 Ser Lys Leu Val Ala Tyr Ala Gln Gly Phe Asp Glu Ile Arg Ala Gly 340 345 350 tcc gcc gag cac gcc tgg gac gtc gac ccc cgc gac ctg gcc acc atc 1404 Ser Ala Glu His Ala Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile 355 360 365 tgg cgg ggc ggc tgc atc atc cgc gcc aag ttc ctc aac cgc atc gtc 1452 Trp Arg Gly Gly Cys Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val 370 375 380 gag gcc tac gac gcc gac ccg cag ctg ccg tcc ctg ctt ctc gac gcc 1500 Glu Ala Tyr Asp Ala Asp Pro Gln Leu Pro Ser Leu Leu Leu Asp Ala 385 390 395 400 tac ttc ctc gac gag ctg cgc gac ctc atc gac tcc tgg cgt cgc gtc 1548 Tyr Phe Leu Asp Glu Leu Arg Asp Leu Ile Asp Ser Trp Arg Arg Val 405 410 415 atc gtc gcc gcc acc cag ctg ggc ctg ccg gtg ccg gtg ttc gcg tcc 1596 Ile Val Ala Ala Thr Gln Leu Gly Leu Pro Val Pro Val Phe Ala Ser 420 425 430 tcc ctg tcc tac tac gac tcg ctg cgc gcc gag cgt ctg ccg gcc gcc 1644 Ser Leu Ser Tyr Tyr Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala 435 440 445 gtg atc cag ggc cag cgt gac tac ttc ggt gcg cac acc tac ggg cgc 1692 Val Ile Gln Gly Gln Arg Asp Tyr Phe Gly Ala His Thr Tyr Gly Arg 450 455 460 gtc gac aag ccg ggt gcc ttc cac acc ctg tgg tcc ggg gac cgc agc 1740 Val Asp Lys Pro Gly Ala Phe His Thr Leu Trp Ser Gly Asp Arg Ser 465 470 475 480 gag atc gag gtg taa tgcccgtgtg ggcgaggatc tcccgtttcc tgccggcggc 1795 Glu Ile Glu Val ggccatcgtg gtggtgaccg ccagggcagt gcaggaggtc cttgcccacg ggcagtgggc 1855 ggtgtggcgg ctgtcgcggc gggagacccg cctgttcgtc gaggtattcc ggacggtgct 1915 gggcctgttc ctcgtcgtcg gcctcacccg ggtggtcgcc gcgcctccct cccccacgga 1975 actggccgga tgggcgctca ccttggggtt cctgtggatc ctcaccctca cggagacgga 2035 acccggcccc gaccccgagc 2055 <210> 174 <211> 484 <212> PRT <213> Corynebacterium humireducens <400> 174 Met Thr Ser Pro Glu Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala 1 5 10 15 Val Met Gly Ser Asn Leu Ala Arg Asn Phe Ala Arg Asn Gly His Thr 20 25 30 Val Ala Val Tyr Asn Arg Ser Pro Glu Lys Thr Arg Asp Leu Ile Ala 35 40 45 Glu His Gly Asp Glu Gly Thr Phe Leu Ala Ser Glu Thr Val Glu Asp 50 55 60 Phe Val Ala Ser Leu Glu Arg Pro Arg Lys Ala Ile Ile Met Val Gln 65 70 75 80 Ala Gly Ala Ala Thr Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met 85 90 95 Glu Asp Gly Asp Ile Ile Ile Asp Gly Gly Asn Ala Leu Tyr Ser Asp 100 105 110 Thr Ile Arg Arg Glu Arg Glu Met Ala Glu Arg Asn Arg His Phe Val 115 120 125 Gly Ala Gly Ile Ser Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser 130 135 140 Ile Met Pro Gly Gly Pro Lys Glu Ser Trp Glu Thr Leu Gly Pro Leu 145 150 155 160 Leu Glu Ser Val Ala Ala Val Val Asp Gly Thr Pro Cys Val Thr His 165 170 175 Ile Gly Pro Asp Gly Ala Gly His Phe Val Lys Met Val His Asn Gly 180 185 190 Ile Glu Tyr Ala Asp Met Gln Val Ile Gly Glu Ala Tyr Gln Leu Leu 195 200 205 Arg Tyr Gly Ala Gly Met Ser Pro Ala Glu Ile Ser Glu Val Phe Arg 210 215 220 Glu Trp Asn Ser Gly Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala 225 230 235 240 Glu Val Leu Ala Gln Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp 245 250 255 Val Ile Val Asp Ala Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val 260 265 270 Lys Ala Ala Leu Asp Leu Gly Ile Pro Val Thr Gly Ile Gly Glu Ala 275 280 285 Val Phe Ala Arg Ala Leu Ser Ser Ser Leu Glu Gln Arg Ala Ala Ala 290 295 300 Gln Gly Val Leu Pro Ala Gly Glu Leu Ala Thr Phe Glu Ser Leu Gly 305 310 315 320 Leu Asp Arg Asp Ala Phe Ile Glu Asp Val Arg Arg Ala Leu Tyr Ala 325 330 335 Ser Lys Leu Val Ala Tyr Ala Gln Gly Phe Asp Glu Ile Arg Ala Gly 340 345 350 Ser Ala Glu His Ala Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile 355 360 365 Trp Arg Gly Gly Cys Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val 370 375 380 Glu Ala Tyr Asp Ala Asp Pro Gln Leu Pro Ser Leu Leu Leu Asp Ala 385 390 395 400 Tyr Phe Leu Asp Glu Leu Arg Asp Leu Ile Asp Ser Trp Arg Arg Val 405 410 415 Ile Val Ala Ala Thr Gln Leu Gly Leu Pro Val Pro Val Phe Ala Ser 420 425 430 Ser Leu Ser Tyr Tyr Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala 435 440 445 Val Ile Gln Gly Gln Arg Asp Tyr Phe Gly Ala His Thr Tyr Gly Arg 450 455 460 Val Asp Lys Pro Gly Ala Phe His Thr Leu Trp Ser Gly Asp Arg Ser 465 470 475 480 Glu Ile Glu Val <210> 175 <211> 2103 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1803) <223> gene mqo <400> 175 cgagaatgtc ctcataccag tcggagaagg cgggaacgct catgatgacc acactagtgg 60 gggtacgggg gcagggggcg ggggagtgag gggcgtcgat aagttcgtgt tttccggacc 120 ttcgtcacac ccccgtgaac ctaaggcgta aggttgatgt ttaccggaca aggggagcgg 180 atctgtgccg aggacgtcag agtccgcacc cggaattgtc ctgcccaccg ggagtagtgc 240 cgacacccgg tgtgggccat gactggccag aaccttcgac gacgaatgag gtaatgcaag 300 gtg tcc acc gaa aac aag aac gct ccc aag gtc act gac gag gtc gat 348 Val Ser Thr Glu Asn Lys Asn Ala Pro Lys Val Thr Asp Glu Val Asp 1 5 10 15 gtc gcc ctc gtc ggc gcg ggt gtc atg agc gcc acc ctg ggt gcg atg 396 Val Ala Leu Val Gly Ala Gly Val Met Ser Ala Thr Leu Gly Ala Met 20 25 30 atc cgc gag ctc gag ccg agc tgg tcc cag atg atc ttc gag cgc ctg 444 Ile Arg Glu Leu Glu Pro Ser Trp Ser Gln Met Ile Phe Glu Arg Leu 35 40 45 gac ggc ccc gcc cag gag tcc tcc tcc ccg tgg aac aac gcc ggt acc 492 Asp Gly Pro Ala Gln Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr 50 55 60 ggc cac tcc gca ctc tgc gag ctg aac tac acc ccc gag gtc aac ggc 540 Gly His Ser Ala Leu Cys Glu Leu Asn Tyr Thr Pro Glu Val Asn Gly 65 70 75 80 aag atc gag gtg gcc aag gcc atc ggc atc aac gag aag ttc cag gtc 588 Lys Ile Glu Val Ala Lys Ala Ile Gly Ile Asn Glu Lys Phe Gln Val 85 90 95 tcc cgc cag ttc tgg tcc cac cag atc gac cac ggc gtg ctg gac gac 636 Ser Arg Gln Phe Trp Ser His Gln Ile Asp His Gly Val Leu Asp Asp 100 105 110 ccc aag gag tgg atc aac ccc gtc gcc cac ctg gcc ttc gcc cag ggc 684 Pro Lys Glu Trp Ile Asn Pro Val Ala His Leu Ala Phe Ala Gln Gly 115 120 125 gac gac cag gtc gac tac ctc aag cgc cgc cac gag gcg ctg gcc gac 732 Asp Asp Gln Val Asp Tyr Leu Lys Arg Arg His Glu Ala Leu Ala Asp 130 135 140 aac atc ctc ttc ccg ggc atg gag tgg gtc gag gac gac gcc aag ttc 780 Asn Ile Leu Phe Pro Gly Met Glu Trp Val Glu Asp Asp Ala Lys Phe 145 150 155 160 gcc gag cgc ctc ccg gtc atg tcc gag ggc cgt gac ttc gcc gcc gag 828 Ala Glu Arg Leu Pro Val Met Ser Glu Gly Arg Asp Phe Ala Ala Glu 165 170 175 aag gtc gcc tac tcc ggc acc gac atc ggc acc gac gtc aac ttc ggt 876 Lys Val Ala Tyr Ser Gly Thr Asp Ile Gly Thr Asp Val Asn Phe Gly 180 185 190 gcc ctg acc gag cag ttc ctc act gcc gcc gag gcc tcc ggc acg gag 924 Ala Leu Thr Glu Gln Phe Leu Thr Ala Ala Glu Ala Ser Gly Thr Glu 195 200 205 atc cgc tac ggc cac gag gtc aag aac atc aag aag gac ggt ggc aag 972 Ile Arg Tyr Gly His Glu Val Lys Asn Ile Lys Lys Asp Gly Gly Lys 210 215 220 tgg aag atc acc gtc aag aac gtc cac acg ggt gac acc tcc gtg gtc 1020 Trp Lys Ile Thr Val Lys Asn Val His Thr Gly Asp Thr Ser Val Val 225 230 235 240 aag gcc aag ttc gtg ttc gtc ggc gcc ggc ggc tac gcc ctc gac ctg 1068 Lys Ala Lys Phe Val Phe Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu 245 250 255 ctg cgc aag gca ggc gtc ccg gag gtc agc ggt tac gcc ggc ttc ccg 1116 Leu Arg Lys Ala Gly Val Pro Glu Val Ser Gly Tyr Ala Gly Phe Pro 260 265 270 gtg tcc ggc ctg tgg ctg cgc tgc acc aac gag gag ctg atc aac cag 1164 Val Ser Gly Leu Trp Leu Arg Cys Thr Asn Glu Glu Leu Ile Asn Gln 275 280 285 cac gac gcc aag gtc tac ggc aag gcc aag gtc ggt gcc ccg ccg atg 1212 His Asp Ala Lys Val Tyr Gly Lys Ala Lys Val Gly Ala Pro Pro Met 290 295 300 tcc gtc ccg cac ctt gac acc cgc gtc atc gac ggc aag aag ggc ctc 1260 Ser Val Pro His Leu Asp Thr Arg Val Ile Asp Gly Lys Lys Gly Leu 305 310 315 320 ctg ttc ggc ccc tac ggc ggc tgg agc ccg aag ttc ctc aag gag ggt 1308 Leu Phe Gly Pro Tyr Gly Gly Trp Ser Pro Lys Phe Leu Lys Glu Gly 325 330 335 tcg tac ctc gac ctg ttc aag tcg atc cgc ctg gac aac ctc ccg tcc 1356 Ser Tyr Leu Asp Leu Phe Lys Ser Ile Arg Leu Asp Asn Leu Pro Ser 340 345 350 tac ctc ggt gtc gcc gcc cag gag ttc gcc ctg acc aag tac ctc atc 1404 Tyr Leu Gly Val Ala Ala Gln Glu Phe Ala Leu Thr Lys Tyr Leu Ile 355 360 365 acc gag gtg gcg aag gac ttc gag aag cgc ctc gtc gac ctg cgt gac 1452 Thr Glu Val Ala Lys Asp Phe Glu Lys Arg Leu Val Asp Leu Arg Asp 370 375 380 tac gtc ccg tcc gcc aag gcc gag gac tgg gag acc gtg gtc gcc ggt 1500 Tyr Val Pro Ser Ala Lys Ala Glu Asp Trp Glu Thr Val Val Ala Gly 385 390 395 400 cag cgc gtc cag gtg atc aag ccg gcc cgc gcg ccg cgc ttc ggc tcc 1548 Gln Arg Val Gln Val Ile Lys Pro Ala Arg Ala Pro Arg Phe Gly Ser 405 410 415 ctc gag ttc ggc acc gcc ctg atc aac agc acc gac ggc tcc atc gcc 1596 Leu Glu Phe Gly Thr Ala Leu Ile Asn Ser Thr Asp Gly Ser Ile Ala 420 425 430 ggc ctg ctg ggt gcc tcc ccg ggt gcc tcc atc gcc ccg gcc gcc atg 1644 Gly Leu Leu Gly Ala Ser Pro Gly Ala Ser Ile Ala Pro Ala Ala Met 435 440 445 ctc gag ctc gtc gag cgc tgc ttc ggt gac cgc atg atc cag tgg ggc 1692 Leu Glu Leu Val Glu Arg Cys Phe Gly Asp Arg Met Ile Gln Trp Gly 450 455 460 ccg aag ctc aag gag atg atc ccg tcc tac ggc acc aag ctg ggc gac 1740 Pro Lys Leu Lys Glu Met Ile Pro Ser Tyr Gly Thr Lys Leu Gly Asp 465 470 475 480 gac gag aag atg ttc aac gag ctc tgg gac tac acc cag aag acc ctg 1788 Asp Glu Lys Met Phe Asn Glu Leu Trp Asp Tyr Thr Gln Lys Thr Leu 485 490 495 aag ctc gag aag tag ttccactgtg agggccgcga ccaccgggtc gcggccttcg 1843 Lys Leu Glu Lys 500 ccgattccgt aggctggtgg ccatgcaacg catcgtgccc accatctggt tcaaccggaa 1903 cgccgacgag gccgccgagt tctacgccga cgtcttcccc gacacgcttg tcgacgcccg 1963 cgtcccccac cccgaatccg gcgagaccct caccgtcgac gtgctcatcg acggctaccg 2023 cgtcaccctc ctcaacggtg ggccggagtt cggcccgacc cccgcgatca gcttcttcgt 2083 caacttcgac ccctcccgca 2103 <210> 176 <211> 500 <212> PRT <213> Corynebacterium humireducens <400> 176 Val Ser Thr Glu Asn Lys Asn Ala Pro Lys Val Thr Asp Glu Val Asp 1 5 10 15 Val Ala Leu Val Gly Ala Gly Val Met Ser Ala Thr Leu Gly Ala Met 20 25 30 Ile Arg Glu Leu Glu Pro Ser Trp Ser Gln Met Ile Phe Glu Arg Leu 35 40 45 Asp Gly Pro Ala Gln Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr 50 55 60 Gly His Ser Ala Leu Cys Glu Leu Asn Tyr Thr Pro Glu Val Asn Gly 65 70 75 80 Lys Ile Glu Val Ala Lys Ala Ile Gly Ile Asn Glu Lys Phe Gln Val 85 90 95 Ser Arg Gln Phe Trp Ser His Gln Ile Asp His Gly Val Leu Asp Asp 100 105 110 Pro Lys Glu Trp Ile Asn Pro Val Ala His Leu Ala Phe Ala Gln Gly 115 120 125 Asp Asp Gln Val Asp Tyr Leu Lys Arg Arg His Glu Ala Leu Ala Asp 130 135 140 Asn Ile Leu Phe Pro Gly Met Glu Trp Val Glu Asp Asp Ala Lys Phe 145 150 155 160 Ala Glu Arg Leu Pro Val Met Ser Glu Gly Arg Asp Phe Ala Ala Glu 165 170 175 Lys Val Ala Tyr Ser Gly Thr Asp Ile Gly Thr Asp Val Asn Phe Gly 180 185 190 Ala Leu Thr Glu Gln Phe Leu Thr Ala Ala Glu Ala Ser Gly Thr Glu 195 200 205 Ile Arg Tyr Gly His Glu Val Lys Asn Ile Lys Lys Asp Gly Gly Lys 210 215 220 Trp Lys Ile Thr Val Lys Asn Val His Thr Gly Asp Thr Ser Val Val 225 230 235 240 Lys Ala Lys Phe Val Phe Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu 245 250 255 Leu Arg Lys Ala Gly Val Pro Glu Val Ser Gly Tyr Ala Gly Phe Pro 260 265 270 Val Ser Gly Leu Trp Leu Arg Cys Thr Asn Glu Glu Leu Ile Asn Gln 275 280 285 His Asp Ala Lys Val Tyr Gly Lys Ala Lys Val Gly Ala Pro Pro Met 290 295 300 Ser Val Pro His Leu Asp Thr Arg Val Ile Asp Gly Lys Lys Gly Leu 305 310 315 320 Leu Phe Gly Pro Tyr Gly Gly Trp Ser Pro Lys Phe Leu Lys Glu Gly 325 330 335 Ser Tyr Leu Asp Leu Phe Lys Ser Ile Arg Leu Asp Asn Leu Pro Ser 340 345 350 Tyr Leu Gly Val Ala Ala Gln Glu Phe Ala Leu Thr Lys Tyr Leu Ile 355 360 365 Thr Glu Val Ala Lys Asp Phe Glu Lys Arg Leu Val Asp Leu Arg Asp 370 375 380 Tyr Val Pro Ser Ala Lys Ala Glu Asp Trp Glu Thr Val Val Ala Gly 385 390 395 400 Gln Arg Val Gln Val Ile Lys Pro Ala Arg Ala Pro Arg Phe Gly Ser 405 410 415 Leu Glu Phe Gly Thr Ala Leu Ile Asn Ser Thr Asp Gly Ser Ile Ala 420 425 430 Gly Leu Leu Gly Ala Ser Pro Gly Ala Ser Ile Ala Pro Ala Ala Met 435 440 445 Leu Glu Leu Val Glu Arg Cys Phe Gly Asp Arg Met Ile Gln Trp Gly 450 455 460 Pro Lys Leu Lys Glu Met Ile Pro Ser Tyr Gly Thr Lys Leu Gly Asp 465 470 475 480 Asp Glu Lys Met Phe Asn Glu Leu Trp Asp Tyr Thr Gln Lys Thr Leu 485 490 495 Lys Leu Glu Lys 500 <210> 177 <211> 3357 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(3057) <223> gene aceE <400> 177 gtgggagatg accctcgttg ccgccccttg tgggctggtt gtgacctcgg gggtgggagc 60 cggggtttgc aatctccgcc acacacctcg cggagaggcg ggacaggcct gcgacaaggg 120 gcgatgggac gtcgataagc ggaacgccgg acccgttcat cttcgcaagc tttacaggaa 180 tgcagaacta cgttggccaa tgaaaacacg tatcctggac tggacactcg ggcctgtccg 240 gaccaccttg ccggcgggcc cgttgctcca ccacgacacc cagaaacctg gaggcacatc 300 atg gca gat gac ctg aac gcg aac ggc cgc aag ggc aac tcc cac tac 348 Met Ala Asp Asp Leu Asn Ala Asn Gly Arg Lys Gly Asn Ser His Tyr 1 5 10 15 ccg gcg atc cgc gac ggc gtc gcg tcc tac ctc aaa gac acc gac ccg 396 Pro Ala Ile Arg Asp Gly Val Ala Ser Tyr Leu Lys Asp Thr Asp Pro 20 25 30 gag gag acc cag gag tgg atg gac tcc ctc gac ggt ctc ctc gcg gag 444 Glu Glu Thr Gln Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Ala Glu 35 40 45 aag gac ccg gcc cgc gcc cgc tac ctc atg ctc cgc atg ctg gag cgc 492 Lys Asp Pro Ala Arg Ala Arg Tyr Leu Met Leu Arg Met Leu Glu Arg 50 55 60 gcg gcc ctc aag cgt gtc ccg ctg ccg aac ctg acc tcc aca gac ttc 540 Ala Ala Leu Lys Arg Val Pro Leu Pro Asn Leu Thr Ser Thr Asp Phe 65 70 75 80 gtc aac acg atc ccc acc acc atg gag ccg gag ttc ccg ggt gac gag 588 Val Asn Thr Ile Pro Thr Thr Met Glu Pro Glu Phe Pro Gly Asp Glu 85 90 95 gag atg gag aag cgc tac cgc cgc tgg atg cgc tgg aac gcc gcc atc 636 Glu Met Glu Lys Arg Tyr Arg Arg Trp Met Arg Trp Asn Ala Ala Ile 100 105 110 atg gtg cac cgc gcc cag cgc ccc ggc atc ggc gtc ggc ggc cac atc 684 Met Val His Arg Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His Ile 115 120 125 tcc acc tac gcc tcc gcc gcc ccg ctc tac gag gtc ggc ttc aat cac 732 Ser Thr Tyr Ala Ser Ala Ala Pro Leu Tyr Glu Val Gly Phe Asn His 130 135 140 ttc tgg cgc ggc aag gac cac ccg ggc ggc ggc gac cag atc ttc ttc 780 Phe Trp Arg Gly Lys Asp His Pro Gly Gly Gly Asp Gln Ile Phe Phe 145 150 155 160 cag ggc cac gcc tcc ccc ggc atc tac gcc cgc gcc ttc ctc gag ggg 828 Gln Gly His Ala Ser Pro Gly Ile Tyr Ala Arg Ala Phe Leu Glu Gly 165 170 175 cgc ctg agc gag gac gac ctc gac ggc ttc cgc cag gag gtc tcc cgc 876 Arg Leu Ser Glu Asp Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg 180 185 190 ggt gag ggc aac ggc atc ccg tcc tac ccg cac ccg cac ggc atg cct 924 Gly Glu Gly Asn Gly Ile Pro Ser Tyr Pro His Pro His Gly Met Pro 195 200 205 gat ttc tgg gag ttc ccg acc gtc tcc atg gga ctg ggc ccg atg gac 972 Asp Phe Trp Glu Phe Pro Thr Val Ser Met Gly Leu Gly Pro Met Asp 210 215 220 gcg atc tac cag gca cgt ttc aac cgt tac ctg cac aac cgc ggc atc 1020 Ala Ile Tyr Gln Ala Arg Phe Asn Arg Tyr Leu His Asn Arg Gly Ile 225 230 235 240 aag gac acc tcc gac cag cac gtg tgg gcc ttc ctc ggc gac ggc gag 1068 Lys Asp Thr Ser Asp Gln His Val Trp Ala Phe Leu Gly Asp Gly Glu 245 250 255 atg gac gag ccg gag tcc cgc ggc ctc atc cag cac gcc gcg ctg aac 1116 Met Asp Glu Pro Glu Ser Arg Gly Leu Ile Gln His Ala Ala Leu Asn 260 265 270 aac ctg gac aac ctc acc ttc gtg gtc aac tgc aac ctg cag cgt ctc 1164 Asn Leu Asp Asn Leu Thr Phe Val Val Asn Cys Asn Leu Gln Arg Leu 275 280 285 gac ggc ccg gtc cgc ggc aac acg cag atc atc cag gag ctc gag tcc 1212 Asp Gly Pro Val Arg Gly Asn Thr Gln Ile Ile Gln Glu Leu Glu Ser 290 295 300 ttc ttc cgg ggt gcc ggc tgg tcc gtc atc aag gtc atc tgg ggc cgc 1260 Phe Phe Arg Gly Ala Gly Trp Ser Val Ile Lys Val Ile Trp Gly Arg 305 310 315 320 gag tgg gac gcc ctg ctg gag aag gac gag tcc ggc gcg ctg gtc aac 1308 Glu Trp Asp Ala Leu Leu Glu Lys Asp Glu Ser Gly Ala Leu Val Asn 325 330 335 gtc atg aac acc acc ccg gac ggt gac tac cag acc ttc aag gcc aac 1356 Val Met Asn Thr Thr Pro Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn 340 345 350 gac ggc gcc tac gtc cgc gag cac ttc ttc ggc cgc gac gag cgc acc 1404 Asp Gly Ala Tyr Val Arg Glu His Phe Phe Gly Arg Asp Glu Arg Thr 355 360 365 gcc aaa ctc gtc gag gac atg acc gac gag gag atc tgg gcc ctg ccg 1452 Ala Lys Leu Val Glu Asp Met Thr Asp Glu Glu Ile Trp Ala Leu Pro 370 375 380 cgc ggt ggc cac gac tac cgc aag gtc cac gcc gcc tac aag cgg gcg 1500 Arg Gly Gly His Asp Tyr Arg Lys Val His Ala Ala Tyr Lys Arg Ala 385 390 395 400 ctg gag acc aag gac cgc ccg acc gtc atc ctc gcg ttc acc atc aag 1548 Leu Glu Thr Lys Asp Arg Pro Thr Val Ile Leu Ala Phe Thr Ile Lys 405 410 415 ggc tac ggc ctg ggc cac aac ttc gag ggc cgc aac gcg acg cac cag 1596 Gly Tyr Gly Leu Gly His Asn Phe Glu Gly Arg Asn Ala Thr His Gln 420 425 430 atg aag aag ctc gcc gcc gag gac ctg aag aac ttc cgc gac aag cag 1644 Met Lys Lys Leu Ala Ala Glu Asp Leu Lys Asn Phe Arg Asp Lys Gln 435 440 445 tcc atc ccg atc ccg gac gag gtc ctc gag gcc gac ccg tac ctg ccg 1692 Ser Ile Pro Ile Pro Asp Glu Val Leu Glu Ala Asp Pro Tyr Leu Pro 450 455 460 ccg tac tac cac ccg ggc gag gac gcc ccg gag atc cag tac atg aag 1740 Pro Tyr Tyr His Pro Gly Glu Asp Ala Pro Glu Ile Gln Tyr Met Lys 465 470 475 480 aag cgt cgt cag gag ctg ggc ggt ttc atc ccg gag cgt cgc cag acc 1788 Lys Arg Arg Gln Glu Leu Gly Gly Phe Ile Pro Glu Arg Arg Gln Thr 485 490 495 tac acc ccg ctg cag gtc ccg gac atc gag aag ctg cgc agc gtg cgc 1836 Tyr Thr Pro Leu Gln Val Pro Asp Ile Glu Lys Leu Arg Ser Val Arg 500 505 510 aag ggc tcc ggc aag cag gag gtc gcc tcc acc atg gcg ctc gtg cgt 1884 Lys Gly Ser Gly Lys Gln Glu Val Ala Ser Thr Met Ala Leu Val Arg 515 520 525 gcc ttc aag gag ctc atg cgt gac aag gag ctg ggc cgc cgc gtc gtg 1932 Ala Phe Lys Glu Leu Met Arg Asp Lys Glu Leu Gly Arg Arg Val Val 530 535 540 ccg atc atc ccg gac gag gcc cgc acc ttc ggc ctc gac tcc tgg ttc 1980 Pro Ile Ile Pro Asp Glu Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe 545 550 555 560 ccg acc ctg aag atc tac aac ccg cac ggc cag aac tac gtg ccg gtc 2028 Pro Thr Leu Lys Ile Tyr Asn Pro His Gly Gln Asn Tyr Val Pro Val 565 570 575 gac cat gac ctc atg ctg tcc tac cgg gag gcc acc gac ggc cag atc 2076 Asp His Asp Leu Met Leu Ser Tyr Arg Glu Ala Thr Asp Gly Gln Ile 580 585 590 ctc cac gag ggc atc aac gag gcc ggt tcc gtc gcg tcc ttc atg gcc 2124 Leu His Glu Gly Ile Asn Glu Ala Gly Ser Val Ala Ser Phe Met Ala 595 600 605 gcc ggc acc tcc tac gcc acc cac ggc gaa gcg atg atc ccg ctg tac 2172 Ala Gly Thr Ser Tyr Ala Thr His Gly Glu Ala Met Ile Pro Leu Tyr 610 615 620 atc ttc tac tcg atg ttc ggt ttc cag cgc acc ggc gac ggc atc tgg 2220 Ile Phe Tyr Ser Met Phe Gly Phe Gln Arg Thr Gly Asp Gly Ile Trp 625 630 635 640 gcc gcc gcc gac cag atg gcc cgt ggc ttc ctc atc ggc gcc acc gcc 2268 Ala Ala Ala Asp Gln Met Ala Arg Gly Phe Leu Ile Gly Ala Thr Ala 645 650 655 ggc cgc acc acc ctg acg ggt gag ggc ctg cag cac atg gac ggc cac 2316 Gly Arg Thr Thr Leu Thr Gly Glu Gly Leu Gln His Met Asp Gly His 660 665 670 tcg cag atc ctc gcc tcc acg aac ccg ggc gtc gtg tcc tac gac ccg 2364 Ser Gln Ile Leu Ala Ser Thr Asn Pro Gly Val Val Ser Tyr Asp Pro 675 680 685 gcg ttc gcc tac gag atc gcg cac ctg ctc cat gag ggc atc gac cgc 2412 Ala Phe Ala Tyr Glu Ile Ala His Leu Leu His Glu Gly Ile Asp Arg 690 695 700 atg tac ggc ccg ggc cgc ggt gag aac gtc atc tac tac ctc acc gtg 2460 Met Tyr Gly Pro Gly Arg Gly Glu Asn Val Ile Tyr Tyr Leu Thr Val 705 710 715 720 tac aac gag ccg gtc ccg cag ccg gcc gag ccg gag aac ctc gac gtc 2508 Tyr Asn Glu Pro Val Pro Gln Pro Ala Glu Pro Glu Asn Leu Asp Val 725 730 735 gag ggc ctg cac aag ggc atg tac ctg ttc tcc cgc gcg gag gag ctc 2556 Glu Gly Leu His Lys Gly Met Tyr Leu Phe Ser Arg Ala Glu Glu Leu 740 745 750 gag gtc gag gac ggc cac gag gcc tcc atc ctg gcc tcc ggc atc ggc 2604 Glu Val Glu Asp Gly His Glu Ala Ser Ile Leu Ala Ser Gly Ile Gly 755 760 765 atg cag tgg gcg ctc aag gcc cag cag atg ctg gcc gag gag tac aac 2652 Met Gln Trp Ala Leu Lys Ala Gln Gln Met Leu Ala Glu Glu Tyr Asn 770 775 780 gtg cgt gcc aac gtc ttc tcc gtc acc tcc tgg gtc gag ctg gcc cgc 2700 Val Arg Ala Asn Val Phe Ser Val Thr Ser Trp Val Glu Leu Ala Arg 785 790 795 800 gag ggt gcg gcg aag aac aag gag cag ctg cgc aac ccg gcc gca gag 2748 Glu Gly Ala Ala Lys Asn Lys Glu Gln Leu Arg Asn Pro Ala Ala Glu 805 810 815 atc gag gag gcc ttc gcc acc agg cag ctg aag cag gtc gac ggc ggc 2796 Ile Glu Glu Ala Phe Ala Thr Arg Gln Leu Lys Gln Val Asp Gly Gly 820 825 830 acc ttc gtg gcc gtc tcc gac ttc gcg acc gac ctg cag gag cag atc 2844 Thr Phe Val Ala Val Ser Asp Phe Ala Thr Asp Leu Gln Glu Gln Ile 835 840 845 cgc gcc tac gtc ccg ggc gag tac atc gtc ctg ggc gcc gac ggc ttc 2892 Arg Ala Tyr Val Pro Gly Glu Tyr Ile Val Leu Gly Ala Asp Gly Phe 850 855 860 ggc ttc tcc gac acc cgt ccg gcc gcc cgc cgc ttc ttc aac atc gac 2940 Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp 865 870 875 880 gcc gag tcc atc gtc gtc gcg gtc ctc atg ggc ctg gcc cgc gag ggc 2988 Ala Glu Ser Ile Val Val Ala Val Leu Met Gly Leu Ala Arg Glu Gly 885 890 895 aag atc gac atc tct gtg gcc cag cag gcc gcc gag aag tac cag ctg 3036 Lys Ile Asp Ile Ser Val Ala Gln Gln Ala Ala Glu Lys Tyr Gln Leu 900 905 910 gac gat ccg acc gct gtg taa ctgatccccc ggtcaaggcc ctgctccggc 3087 Asp Asp Pro Thr Ala Val 915 agggccttgc tgctttcccg cttgtcgacg cccctctcac tccctgttga ggtaccgcgg 3147 ccaggaggtg tcgcgggtgg ccagcgagat ctccaccgcg gtgtcgagct ccgctgccag 3207 gggctcgggc agcttgtgcc ggagcgcgtc gccgaggcgc gcgatgacct ccaggcgcag 3267 caggttgcgg gtctccgtga tgtagatgcg cgggtaccgg gtggccagct cgacctcgga 3327 ggggtcggcg ggcccgccgt acatctcgac 3357 <210> 178 <211> 918 <212> PRT <213> Corynebacterium humireducens <400> 178 Met Ala Asp Asp Leu Asn Ala Asn Gly Arg Lys Gly Asn Ser His Tyr 1 5 10 15 Pro Ala Ile Arg Asp Gly Val Ala Ser Tyr Leu Lys Asp Thr Asp Pro 20 25 30 Glu Glu Thr Gln Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Ala Glu 35 40 45 Lys Asp Pro Ala Arg Ala Arg Tyr Leu Met Leu Arg Met Leu Glu Arg 50 55 60 Ala Ala Leu Lys Arg Val Pro Leu Pro Asn Leu Thr Ser Thr Asp Phe 65 70 75 80 Val Asn Thr Ile Pro Thr Thr Met Glu Pro Glu Phe Pro Gly Asp Glu 85 90 95 Glu Met Glu Lys Arg Tyr Arg Arg Trp Met Arg Trp Asn Ala Ala Ile 100 105 110 Met Val His Arg Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His Ile 115 120 125 Ser Thr Tyr Ala Ser Ala Ala Pro Leu Tyr Glu Val Gly Phe Asn His 130 135 140 Phe Trp Arg Gly Lys Asp His Pro Gly Gly Gly Asp Gln Ile Phe Phe 145 150 155 160 Gln Gly His Ala Ser Pro Gly Ile Tyr Ala Arg Ala Phe Leu Glu Gly 165 170 175 Arg Leu Ser Glu Asp Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg 180 185 190 Gly Glu Gly Asn Gly Ile Pro Ser Tyr Pro His Pro His Gly Met Pro 195 200 205 Asp Phe Trp Glu Phe Pro Thr Val Ser Met Gly Leu Gly Pro Met Asp 210 215 220 Ala Ile Tyr Gln Ala Arg Phe Asn Arg Tyr Leu His Asn Arg Gly Ile 225 230 235 240 Lys Asp Thr Ser Asp Gln His Val Trp Ala Phe Leu Gly Asp Gly Glu 245 250 255 Met Asp Glu Pro Glu Ser Arg Gly Leu Ile Gln His Ala Ala Leu Asn 260 265 270 Asn Leu Asp Asn Leu Thr Phe Val Val Asn Cys Asn Leu Gln Arg Leu 275 280 285 Asp Gly Pro Val Arg Gly Asn Thr Gln Ile Ile Gln Glu Leu Glu Ser 290 295 300 Phe Phe Arg Gly Ala Gly Trp Ser Val Ile Lys Val Ile Trp Gly Arg 305 310 315 320 Glu Trp Asp Ala Leu Leu Glu Lys Asp Glu Ser Gly Ala Leu Val Asn 325 330 335 Val Met Asn Thr Thr Pro Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn 340 345 350 Asp Gly Ala Tyr Val Arg Glu His Phe Phe Gly Arg Asp Glu Arg Thr 355 360 365 Ala Lys Leu Val Glu Asp Met Thr Asp Glu Glu Ile Trp Ala Leu Pro 370 375 380 Arg Gly Gly His Asp Tyr Arg Lys Val His Ala Ala Tyr Lys Arg Ala 385 390 395 400 Leu Glu Thr Lys Asp Arg Pro Thr Val Ile Leu Ala Phe Thr Ile Lys 405 410 415 Gly Tyr Gly Leu Gly His Asn Phe Glu Gly Arg Asn Ala Thr His Gln 420 425 430 Met Lys Lys Leu Ala Ala Glu Asp Leu Lys Asn Phe Arg Asp Lys Gln 435 440 445 Ser Ile Pro Ile Pro Asp Glu Val Leu Glu Ala Asp Pro Tyr Leu Pro 450 455 460 Pro Tyr Tyr His Pro Gly Glu Asp Ala Pro Glu Ile Gln Tyr Met Lys 465 470 475 480 Lys Arg Arg Gln Glu Leu Gly Gly Phe Ile Pro Glu Arg Arg Gln Thr 485 490 495 Tyr Thr Pro Leu Gln Val Pro Asp Ile Glu Lys Leu Arg Ser Val Arg 500 505 510 Lys Gly Ser Gly Lys Gln Glu Val Ala Ser Thr Met Ala Leu Val Arg 515 520 525 Ala Phe Lys Glu Leu Met Arg Asp Lys Glu Leu Gly Arg Arg Val Val 530 535 540 Pro Ile Ile Pro Asp Glu Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe 545 550 555 560 Pro Thr Leu Lys Ile Tyr Asn Pro His Gly Gln Asn Tyr Val Pro Val 565 570 575 Asp His Asp Leu Met Leu Ser Tyr Arg Glu Ala Thr Asp Gly Gln Ile 580 585 590 Leu His Glu Gly Ile Asn Glu Ala Gly Ser Val Ala Ser Phe Met Ala 595 600 605 Ala Gly Thr Ser Tyr Ala Thr His Gly Glu Ala Met Ile Pro Leu Tyr 610 615 620 Ile Phe Tyr Ser Met Phe Gly Phe Gln Arg Thr Gly Asp Gly Ile Trp 625 630 635 640 Ala Ala Ala Asp Gln Met Ala Arg Gly Phe Leu Ile Gly Ala Thr Ala 645 650 655 Gly Arg Thr Thr Leu Thr Gly Glu Gly Leu Gln His Met Asp Gly His 660 665 670 Ser Gln Ile Leu Ala Ser Thr Asn Pro Gly Val Val Ser Tyr Asp Pro 675 680 685 Ala Phe Ala Tyr Glu Ile Ala His Leu Leu His Glu Gly Ile Asp Arg 690 695 700 Met Tyr Gly Pro Gly Arg Gly Glu Asn Val Ile Tyr Tyr Leu Thr Val 705 710 715 720 Tyr Asn Glu Pro Val Pro Gln Pro Ala Glu Pro Glu Asn Leu Asp Val 725 730 735 Glu Gly Leu His Lys Gly Met Tyr Leu Phe Ser Arg Ala Glu Glu Leu 740 745 750 Glu Val Glu Asp Gly His Glu Ala Ser Ile Leu Ala Ser Gly Ile Gly 755 760 765 Met Gln Trp Ala Leu Lys Ala Gln Gln Met Leu Ala Glu Glu Tyr Asn 770 775 780 Val Arg Ala Asn Val Phe Ser Val Thr Ser Trp Val Glu Leu Ala Arg 785 790 795 800 Glu Gly Ala Ala Lys Asn Lys Glu Gln Leu Arg Asn Pro Ala Ala Glu 805 810 815 Ile Glu Glu Ala Phe Ala Thr Arg Gln Leu Lys Gln Val Asp Gly Gly 820 825 830 Thr Phe Val Ala Val Ser Asp Phe Ala Thr Asp Leu Gln Glu Gln Ile 835 840 845 Arg Ala Tyr Val Pro Gly Glu Tyr Ile Val Leu Gly Ala Asp Gly Phe 850 855 860 Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp 865 870 875 880 Ala Glu Ser Ile Val Val Ala Val Leu Met Gly Leu Ala Arg Glu Gly 885 890 895 Lys Ile Asp Ile Ser Val Ala Gln Gln Ala Ala Glu Lys Tyr Gln Leu 900 905 910 Asp Asp Pro Thr Ala Val 915 <210> 179 <211> 1752 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1452) <223> gene gltA <400> 179 agctgctctc cgcccagcag ctcgaggagc tcggcttcaa cgccgtcatc tacccggtga 60 ccaccctgcg catcgccatg ggacaggtcg aggaggctct cgcggagatc gccgccaccg 120 gcacccagac cgggtgggtc gaccgcatgc agcaccgctc ccgcctctac gaactgctcc 180 gctacaacga gtacaacgcc ttcgaccagg aggttttcac gtactccgcc gacacctacc 240 agcccacctt ccagtagccc accagcaact cctgacattc cttgaaaggg gatgagtacc 300 atg tcc gac aac acc aac atc gag gtc cgc aag ggt ctc tac ggc gtg 348 Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val 1 5 10 15 atc gcc gac tac acc gca gtc tcc aag gtc atg ccg gag acc aac tcc 396 Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser 20 25 30 ctg acc tac cgc gga tac gcc gtc cag gac ctc gtc gca gag tgc tcc 444 Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser 35 40 45 ttc gag gag gtc ttc tac ctc ctg tgg aac ggt gag ctg ccg aac gag 492 Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu 50 55 60 gag cag ctg gcg gag ttc aac agg cgc ggc cgt tcc tac cgc aag ctc 540 Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu 65 70 75 80 gac gcc ggc ctg atc gca ctc atc cac tcc ctg ccg ctc gac tgt cac 588 Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His 85 90 95 ccg atg gac gtc atg cgc acc gcc gtg tcc tac atg ggc acc aag gac 636 Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp 100 105 110 tcc gag cac ttc acc ccg gac gcc gac cac atc acc cac gtc ggc cac 684 Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His 115 120 125 aac ctc ctc gcc cag ctg ccg atg gtg ctg gcc atg gac atc cgc cgc 732 Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg 130 135 140 cgt cag ggc ctc gac atc gtc gcc ccg gac ccg gac aag tcc gtc gcc 780 Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala 145 150 155 160 cac aac ctg ctg tcg atg gtc ttc ggc aac ggc ccg gag tcc ccg gcg 828 His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala 165 170 175 tcc aac ccg gac gac gtg cgt gac ttc gag aag tcg ctc atc ctc tac 876 Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr 180 185 190 gcc gag cac tcc ttc aac gcc tcg acc ttc acc tcg cgt gtg atc acc 924 Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr 195 200 205 tcg acc cgc tcg gac gtc tac tcc gcg atc acc ggt gcc atc ggc gcc 972 Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala 210 215 220 ctc aag ggc ccg ctg cac ggc ggc gcc aac gag ttc gtc atg cac acc 1020 Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr 225 230 235 240 atg ctg gag atc gac gac ccg gag aag gcc gcc gac tgg gtc aac aac 1068 Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn 245 250 255 gcc ctg gac aac aag gac ctc atc atg ggc ttc ggt cac cgc gtg tac 1116 Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr 260 265 270 aag aag ggt gac tcg cgt gtc ccg tcc atg gag gcg tcc ttc cgt gac 1164 Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp 275 280 285 ctc gcc gag cgt cac gac ggc gcc aag tgg gtc gcc atg tac gag aac 1212 Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn 290 295 300 atg cgc gac gcc atg gac gcc cgc acc ggc atc aag ccg aac ctg gac 1260 Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp 305 310 315 320 ttc ccg gcc ggc ccg gcg tac cac ctg ctc ggc ttc ccg gtc gac ttc 1308 Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe 325 330 335 ttc acc ccg ctg ttc gtc gtc gcc cgc atc gcc ggc tgg acc gcc cac 1356 Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His 340 345 350 atc gtg gag cag tac aac gac aac tcc ctg att cgt ccg ctc tcc gcc 1404 Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala 355 360 365 tac aac ggc ccc gag cag cgc cag gtc acc ccg ctg gcc gcc cgt tag 1452 Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg 370 375 380 ccccacggcc ggcaccggac caccgcgacg cccacggcac tgcccacaca ttggggtggt 1512 gcgcgtgggt ttcgctgttc ggaacacctg tcggcccatt ccgcagggcg ggacagaaac 1572 ggcctttcgg gcgcccgtcc cgcggagtgc ggaaatggac cgcaccgggt ccaccacctg 1632 cagatacccc ctgtgactag gggatactcc cccgaagaca accctctttc ttgcgatact 1692 gtggtgtgcc agagagctca cccgctctca gacacacccc cgacatgacg ttcggaaagg 1752 <210> 180 <211> 383 <212> PRT <213> Corynebacterium humireducens <400> 180 Met Ser Asp Asn Thr Asn Ile Glu Val Arg Lys Gly Leu Tyr Gly Val 1 5 10 15 Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser 20 25 30 Leu Thr Tyr Arg Gly Tyr Ala Val Gln Asp Leu Val Ala Glu Cys Ser 35 40 45 Phe Glu Glu Val Phe Tyr Leu Leu Trp Asn Gly Glu Leu Pro Asn Glu 50 55 60 Glu Gln Leu Ala Glu Phe Asn Arg Arg Gly Arg Ser Tyr Arg Lys Leu 65 70 75 80 Asp Ala Gly Leu Ile Ala Leu Ile His Ser Leu Pro Leu Asp Cys His 85 90 95 Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp 100 105 110 Ser Glu His Phe Thr Pro Asp Ala Asp His Ile Thr His Val Gly His 115 120 125 Asn Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg 130 135 140 Arg Gln Gly Leu Asp Ile Val Ala Pro Asp Pro Asp Lys Ser Val Ala 145 150 155 160 His Asn Leu Leu Ser Met Val Phe Gly Asn Gly Pro Glu Ser Pro Ala 165 170 175 Ser Asn Pro Asp Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr 180 185 190 Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ser Arg Val Ile Thr 195 200 205 Ser Thr Arg Ser Asp Val Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala 210 215 220 Leu Lys Gly Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr 225 230 235 240 Met Leu Glu Ile Asp Asp Pro Glu Lys Ala Ala Asp Trp Val Asn Asn 245 250 255 Ala Leu Asp Asn Lys Asp Leu Ile Met Gly Phe Gly His Arg Val Tyr 260 265 270 Lys Lys Gly Asp Ser Arg Val Pro Ser Met Glu Ala Ser Phe Arg Asp 275 280 285 Leu Ala Glu Arg His Asp Gly Ala Lys Trp Val Ala Met Tyr Glu Asn 290 295 300 Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp 305 310 315 320 Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe Pro Val Asp Phe 325 330 335 Phe Thr Pro Leu Phe Val Val Ala Arg Ile Ala Gly Trp Thr Ala His 340 345 350 Ile Val Glu Gln Tyr Asn Asp Asn Ser Leu Ile Arg Pro Leu Ser Ala 355 360 365 Tyr Asn Gly Pro Glu Gln Arg Gln Val Thr Pro Leu Ala Ala Arg 370 375 380 <210> 181 <211> 1578 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1278) <223> gene mdh <400> 181 gaacatgcgg ggtcgtccct ccgggggcac gggtcggggt ggaagggtct ggaagggggc 60 ggtgccggcg ggtctgtgcg ggccgcaacc ggcgctgtga tccatcgtgc ctgatgtatc 120 ggtcggtggt ggggcggtgc ggctgagaag atgctggacc acccgatgta gcgacgttct 180 tccagcggaa acagccggat ctccggcgag gttacgccca tccgggggtg gcggctcgga 240 acggtctttg aggctagact cgcaggcgtt gatgcattag accgctcgaa aggatcccgc 300 atg acc cag aac ccc gtg aag atc gcc gtc acc ggt gcc gcc ggc aac 348 Met Thr Gln Asn Pro Val Lys Ile Ala Val Thr Gly Ala Ala Gly Asn 1 5 10 15 atc gcc tac tcc ctc ctg tgg cgc atc gcc gca ggt gac gtc tac ggc 396 Ile Ala Tyr Ser Leu Leu Trp Arg Ile Ala Ala Gly Asp Val Tyr Gly 20 25 30 cgc gac acc ccg atc gag ctc acc ctc ctc gag atc ccg gcc gcc gta 444 Arg Asp Thr Pro Ile Glu Leu Thr Leu Leu Glu Ile Pro Ala Ala Val 35 40 45 cgc ggc gcc gag ggc gtg gcc atg gag ctc aac gac tcc gcc ttc ccc 492 Arg Gly Ala Glu Gly Val Ala Met Glu Leu Asn Asp Ser Ala Phe Pro 50 55 60 ctc ctg cgc aac atc acc gtc acc gac agc gcc gac gtc gcc ttc gac 540 Leu Leu Arg Asn Ile Thr Val Thr Asp Ser Ala Asp Val Ala Phe Asp 65 70 75 80 ggc gcc aac gcc gcc ttc ctt gtc ggc gcg aag ccg cgc ggt gca ggc 588 Gly Ala Asn Ala Ala Phe Leu Val Gly Ala Lys Pro Arg Gly Ala Gly 85 90 95 gag gag cgc gcc gac ctg ctg aag aac aac ggc ggc atc ttc ggc ccg 636 Glu Glu Arg Ala Asp Leu Leu Lys Asn Asn Gly Gly Ile Phe Gly Pro 100 105 110 cag ggc agg gcc atc aac gac cgc gcc gcg gac gac gtc cgc gtg ctc 684 Gln Gly Arg Ala Ile Asn Asp Arg Ala Ala Asp Asp Val Arg Val Leu 115 120 125 gtc gtc ggc aac ccg gcc aac acc aac gcc ctg atc gcc cag cgc aac 732 Val Val Gly Asn Pro Ala Asn Thr Asn Ala Leu Ile Ala Gln Arg Asn 130 135 140 gcc ccg gac gtc ccg gcc gag cgt ttc acc gcg ctg atg cgc ctg gac 780 Ala Pro Asp Val Pro Ala Glu Arg Phe Thr Ala Leu Met Arg Leu Asp 145 150 155 160 cac agc cgt gcg ctg tcc cag ctg gcc gag aag ctc ggc gtg ctc acc 828 His Ser Arg Ala Leu Ser Gln Leu Ala Glu Lys Leu Gly Val Leu Thr 165 170 175 acc gac ttc gag aac gtc gtc gtg tgg ggc aac cac tcc gcg acc cag 876 Thr Asp Phe Glu Asn Val Val Val Trp Gly Asn His Ser Ala Thr Gln 180 185 190 ttc ccg gac gtg acc ttc gcc acc gtc aag ggt gag ccg gtc atc gag 924 Phe Pro Asp Val Thr Phe Ala Thr Val Lys Gly Glu Pro Val Ile Glu 195 200 205 aag atc gac cat gac tgg tac gtc aac gag ttc atc ccg cgc gtc gcg 972 Lys Ile Asp His Asp Trp Tyr Val Asn Glu Phe Ile Pro Arg Val Ala 210 215 220 aag cgt ggc gcc gag atc atc gag gtc cgc ggc aag tcc tcc gcc gcc 1020 Lys Arg Gly Ala Glu Ile Ile Glu Val Arg Gly Lys Ser Ser Ala Ala 225 230 235 240 tcc gcc gcc tcc gcc gcg gtg gac cac atg cgt gac tgg gtc cag ggc 1068 Ser Ala Ala Ser Ala Ala Val Asp His Met Arg Asp Trp Val Gln Gly 245 250 255 act gag cgg tgg gcc tcc gcc gcc ctg ccg tcc gac ggc tcc tac ggc 1116 Thr Glu Arg Trp Ala Ser Ala Ala Leu Pro Ser Asp Gly Ser Tyr Gly 260 265 270 atc gac gag ggt ctc gtc gcc ggc ctg ccg acc gtc tcc cgc aac ggc 1164 Ile Asp Glu Gly Leu Val Ala Gly Leu Pro Thr Val Ser Arg Asn Gly 275 280 285 tcc tgg gag atc gtc cag ggc ctc gag ctc aac gac ttc cag cgc gag 1212 Ser Trp Glu Ile Val Gln Gly Leu Glu Leu Asn Asp Phe Gln Arg Glu 290 295 300 atg atc gcc cgg aac gtc gcg gag ctc aag gcc gag cgt gac gca gtc 1260 Met Ile Ala Arg Asn Val Ala Glu Leu Lys Ala Glu Arg Asp Ala Val 305 310 315 320 gct gac ctg ctt cct tag cgcgtcaact cgcatccgcc gccaatcccg 1308 Ala Asp Leu Leu Pro 325 gtagtgttca ggaacatgcg cttcgggatt gatttcggaa ccacccgtac cgtcattgcg 1368 gcggtcgacc ggggaaacta cccggtcgtc aacgtcacgg acacggacgg cgactcacgg 1428 gagtacatcc cctcggtcgt cgcgctggat ggtgaacggt tggtcggtgg ctgggaggcg 1488 ctggcggtag gtggcccgac cctcacccgc tccttcaagc gtctgctgac ctcccgcacc 1548 gtcaccgcgg agacccccgt ccggctgggc 1578 <210> 182 <211> 325 <212> PRT <213> Corynebacterium humireducens <400> 182 Met Thr Gln Asn Pro Val Lys Ile Ala Val Thr Gly Ala Ala Gly Asn 1 5 10 15 Ile Ala Tyr Ser Leu Leu Trp Arg Ile Ala Ala Gly Asp Val Tyr Gly 20 25 30 Arg Asp Thr Pro Ile Glu Leu Thr Leu Leu Glu Ile Pro Ala Ala Val 35 40 45 Arg Gly Ala Glu Gly Val Ala Met Glu Leu Asn Asp Ser Ala Phe Pro 50 55 60 Leu Leu Arg Asn Ile Thr Val Thr Asp Ser Ala Asp Val Ala Phe Asp 65 70 75 80 Gly Ala Asn Ala Ala Phe Leu Val Gly Ala Lys Pro Arg Gly Ala Gly 85 90 95 Glu Glu Arg Ala Asp Leu Leu Lys Asn Asn Gly Gly Ile Phe Gly Pro 100 105 110 Gln Gly Arg Ala Ile Asn Asp Arg Ala Ala Asp Asp Val Arg Val Leu 115 120 125 Val Val Gly Asn Pro Ala Asn Thr Asn Ala Leu Ile Ala Gln Arg Asn 130 135 140 Ala Pro Asp Val Pro Ala Glu Arg Phe Thr Ala Leu Met Arg Leu Asp 145 150 155 160 His Ser Arg Ala Leu Ser Gln Leu Ala Glu Lys Leu Gly Val Leu Thr 165 170 175 Thr Asp Phe Glu Asn Val Val Val Trp Gly Asn His Ser Ala Thr Gln 180 185 190 Phe Pro Asp Val Thr Phe Ala Thr Val Lys Gly Glu Pro Val Ile Glu 195 200 205 Lys Ile Asp His Asp Trp Tyr Val Asn Glu Phe Ile Pro Arg Val Ala 210 215 220 Lys Arg Gly Ala Glu Ile Ile Glu Val Arg Gly Lys Ser Ser Ala Ala 225 230 235 240 Ser Ala Ala Ser Ala Ala Val Asp His Met Arg Asp Trp Val Gln Gly 245 250 255 Thr Glu Arg Trp Ala Ser Ala Ala Leu Pro Ser Asp Gly Ser Tyr Gly 260 265 270 Ile Asp Glu Gly Leu Val Ala Gly Leu Pro Thr Val Ser Arg Asn Gly 275 280 285 Ser Trp Glu Ile Val Gln Gly Leu Glu Leu Asn Asp Phe Gln Arg Glu 290 295 300 Met Ile Ala Arg Asn Val Ala Glu Leu Lys Ala Glu Arg Asp Ala Val 305 310 315 320 Ala Asp Leu Leu Pro 325 <210> 183 <211> 2100 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1800) <223> gene murE <400> 183 gccggcaaga cgggtacggc ccagaaggtc gacccgcgga cgggcgcgta ctcgaactcg 60 gactactgga tcaccttcgc cggcatcgcc ccggcggatg acccgcgctt cgtcgtcgcc 120 gtcatggtcg acgagccccg cgccggcgtc gaggcgggag gcggcggtgg ccagtcctcg 180 gcgccgttgt tccgtgacat cgcgtcctgg ctgctcaacc gggacaacat cccggcgtca 240 ccgcccatgg aggacatgct gatcctccac gcccactaac ccagcccacc acggaggagt 300 atg acc atg gag acc aca gtg acc aca ctg tcc cac ctc gcc cgt atc 348 Met Thr Met Glu Thr Thr Val Thr Thr Leu Ser His Leu Ala Arg Ile 1 5 10 15 gcc ggg gga gag ctg acc gga tcc gtg gcc ccg gtc gtc acg tcg gtg 396 Ala Gly Gly Glu Leu Thr Gly Ser Val Ala Pro Val Val Thr Ser Val 20 25 30 gag ctc gac tcc tcc cgc ctc gcg gac gag ggc gcg ctc ttc gcc gcc 444 Glu Leu Asp Ser Ser Arg Leu Ala Asp Glu Gly Ala Leu Phe Ala Ala 35 40 45 gtc ccc ggc acg cgc cgc cac ggt gcg gag ttc gcg cgc ggc acg ccg 492 Val Pro Gly Thr Arg Arg His Gly Ala Glu Phe Ala Arg Gly Thr Pro 50 55 60 gcc gtc gcg atc ctc acc gac gcc gcc gga aag gag ctt ctc gac gcc 540 Ala Val Ala Ile Leu Thr Asp Ala Ala Gly Lys Glu Leu Leu Asp Ala 65 70 75 80 gcc ggc gac acc cgc cct gtc atc ctc gtc gat gac gtc cgc gcg gtc 588 Ala Gly Asp Thr Arg Pro Val Ile Leu Val Asp Asp Val Arg Ala Val 85 90 95 ctc ggg gcc gtg gcc gcg gag atc cac ggc cac ccc tcg gag cgt ctc 636 Leu Gly Ala Val Ala Ala Glu Ile His Gly His Pro Ser Glu Arg Leu 100 105 110 acc ctg ctc ggc atc acc ggc acc tcc ggg aag acg acc acc agc tac 684 Thr Leu Leu Gly Ile Thr Gly Thr Ser Gly Lys Thr Thr Thr Ser Tyr 115 120 125 ctg ctc gag cag ggg ctc atg gcg gcc gac ctg aag gtc ggc ctc atc 732 Leu Leu Glu Gln Gly Leu Met Ala Ala Asp Leu Lys Val Gly Leu Ile 130 135 140 ggc acg acg ggc acg cgc atc aac ggc cgt ccc gtc ccc acc gac ctc 780 Gly Thr Thr Gly Thr Arg Ile Asn Gly Arg Pro Val Pro Thr Asp Leu 145 150 155 160 acg acc ccg gag gcc acg acc ctg cag ccc ctg ttc gtg cgg atg ctc 828 Thr Thr Pro Glu Ala Thr Thr Leu Gln Pro Leu Phe Val Arg Met Leu 165 170 175 gcg gag ggc gtc acg cac gtg gtc atg gag gtc tcc tcc cac gcc ctg 876 Ala Glu Gly Val Thr His Val Val Met Glu Val Ser Ser His Ala Leu 180 185 190 gag ctg ggc cgg gtg gcg ggc acg cgt ttc gac gtc gcc ggg ttc acc 924 Glu Leu Gly Arg Val Ala Gly Thr Arg Phe Asp Val Ala Gly Phe Thr 195 200 205 aac ctc tcc cag gac cac ctc gac ttc cac ccg acg atg gag gag tac 972 Asn Leu Ser Gln Asp His Leu Asp Phe His Pro Thr Met Glu Glu Tyr 210 215 220 ttc gcg gcc aag gcc cgc ttc ttc gac ccg gcg tcg ccg ctg gcc gcg 1020 Phe Ala Ala Lys Ala Arg Phe Phe Asp Pro Ala Ser Pro Leu Ala Ala 225 230 235 240 gag cgg tcc gtc gtc tgc gtc gac gac gag tgg ggg cgg cgg atg gcc 1068 Glu Arg Ser Val Val Cys Val Asp Asp Glu Trp Gly Arg Arg Met Ala 245 250 255 gac ctg gcc tcg gac ccg gtg acc gtc gcg acc cgc ggc gcg gag gcc 1116 Asp Leu Ala Ser Asp Pro Val Thr Val Ala Thr Arg Gly Ala Glu Ala 260 265 270 gac gtc acc gcc gtc cag agc tcg ctc acg ccc acc ggc gcc cag gtc 1164 Asp Val Thr Ala Val Gln Ser Ser Leu Thr Pro Thr Gly Ala Gln Val 275 280 285 atc cgg ctc acc ctg ccg gag ggg gcc gtc gag gcg gag ctg ccg ctg 1212 Ile Arg Leu Thr Leu Pro Glu Gly Ala Val Glu Ala Glu Leu Pro Leu 290 295 300 ccg ggt gac ttc aac atc gcc aac gcc gcc ctg gcc acc gcc atg gcg 1260 Pro Gly Asp Phe Asn Ile Ala Asn Ala Ala Leu Ala Thr Ala Met Ala 305 310 315 320 tgg gcc gtg ggc gtc gac ccg cag gcc ttc acc gcc ggt ttc ctc gac 1308 Trp Ala Val Gly Val Asp Pro Gln Ala Phe Thr Ala Gly Phe Leu Asp 325 330 335 gtc gcg gtc ccc ggg cgc atg gag cgt atc gac gag ggc cag gac ttc 1356 Val Ala Val Pro Gly Arg Met Glu Arg Ile Asp Glu Gly Gln Asp Phe 340 345 350 ctc gcc gtc gtc gac tac gcc cac aag ccg gcc gcc gtc gcc gcc gtc 1404 Leu Ala Val Val Asp Tyr Ala His Lys Pro Ala Ala Val Ala Ala Val 355 360 365 ctc gac acc ctc cgc ggg cag gtc cgc ggc cgc ctc ggc gtg gtc gtc 1452 Leu Asp Thr Leu Arg Gly Gln Val Arg Gly Arg Leu Gly Val Val Val 370 375 380 ggg gcc ggt ggt gac cgc gac gcc acc aag cgt ccc ctc atg ggg gcg 1500 Gly Ala Gly Gly Asp Arg Asp Ala Thr Lys Arg Pro Leu Met Gly Ala 385 390 395 400 gag gcc gcc cac cgc gcc gac ctg gtc atc atc acc gac gac aac ccg 1548 Glu Ala Ala His Arg Ala Asp Leu Val Ile Ile Thr Asp Asp Asn Pro 405 410 415 cgc agc gag gtg ccc gcc acc atc cgg gcg gcc gtg ctg cag ggg gcg 1596 Arg Ser Glu Val Pro Ala Thr Ile Arg Ala Ala Val Leu Gln Gly Ala 420 425 430 gtc gac gcc ggg acg gac gcg gag atc cgc gag atc ggg gac cgc gcc 1644 Val Asp Ala Gly Thr Asp Ala Glu Ile Arg Glu Ile Gly Asp Arg Ala 435 440 445 gcc gcc atc gac gcg ctg atc gag tgg gcc cgc ccg ggg gac ggc gtc 1692 Ala Ala Ile Asp Ala Leu Ile Glu Trp Ala Arg Pro Gly Asp Gly Val 450 455 460 gtc gtc gcc ggc aag ggc cac gag gtc ggc cag ctg gtc aac ggc gtc 1740 Val Val Ala Gly Lys Gly His Glu Val Gly Gln Leu Val Asn Gly Val 465 470 475 480 aac cac cat ttc gac gac cgg gaa gag gtc cgg cgc gca ctc aag gag 1788 Asn His His Phe Asp Asp Arg Glu Glu Val Arg Arg Ala Leu Lys Glu 485 490 495 aag aag cat tga tccccctcac tctcgcggag atcgcggaca tcaccggcgg 1840 Lys Lys His tgaactctcg cacgtgaccg acccggagac ccgggtgacc gggtccgtcg agttcgactc 1900 ccgccgggtc accccgggcg ggctgttcct cgccctgccg ggggcgcgcg tcgacggaca 1960 cgacttcgcc gccaccgccg tcgagtccgg cgcggtcgcc gtcctggcgg cgcggccggt 2020 gggggtgccg gcggtcgtcg tgaagccgcg gggccgcagt gactccaacg ccgacatcta 2080 cgcccacgac gaggacggct 2100 <210> 184 <211> 499 <212> PRT <213> Corynebacterium humireducens <400> 184 Met Thr Met Glu Thr Thr Val Thr Thr Leu Ser His Leu Ala Arg Ile 1 5 10 15 Ala Gly Gly Glu Leu Thr Gly Ser Val Ala Pro Val Val Thr Ser Val 20 25 30 Glu Leu Asp Ser Ser Arg Leu Ala Asp Glu Gly Ala Leu Phe Ala Ala 35 40 45 Val Pro Gly Thr Arg Arg His Gly Ala Glu Phe Ala Arg Gly Thr Pro 50 55 60 Ala Val Ala Ile Leu Thr Asp Ala Ala Gly Lys Glu Leu Leu Asp Ala 65 70 75 80 Ala Gly Asp Thr Arg Pro Val Ile Leu Val Asp Asp Val Arg Ala Val 85 90 95 Leu Gly Ala Val Ala Ala Glu Ile His Gly His Pro Ser Glu Arg Leu 100 105 110 Thr Leu Leu Gly Ile Thr Gly Thr Ser Gly Lys Thr Thr Thr Ser Tyr 115 120 125 Leu Leu Glu Gln Gly Leu Met Ala Ala Asp Leu Lys Val Gly Leu Ile 130 135 140 Gly Thr Thr Gly Thr Arg Ile Asn Gly Arg Pro Val Pro Thr Asp Leu 145 150 155 160 Thr Thr Pro Glu Ala Thr Thr Leu Gln Pro Leu Phe Val Arg Met Leu 165 170 175 Ala Glu Gly Val Thr His Val Val Met Glu Val Ser Ser His Ala Leu 180 185 190 Glu Leu Gly Arg Val Ala Gly Thr Arg Phe Asp Val Ala Gly Phe Thr 195 200 205 Asn Leu Ser Gln Asp His Leu Asp Phe His Pro Thr Met Glu Glu Tyr 210 215 220 Phe Ala Ala Lys Ala Arg Phe Phe Asp Pro Ala Ser Pro Leu Ala Ala 225 230 235 240 Glu Arg Ser Val Val Cys Val Asp Asp Glu Trp Gly Arg Arg Met Ala 245 250 255 Asp Leu Ala Ser Asp Pro Val Thr Val Ala Thr Arg Gly Ala Glu Ala 260 265 270 Asp Val Thr Ala Val Gln Ser Ser Leu Thr Pro Thr Gly Ala Gln Val 275 280 285 Ile Arg Leu Thr Leu Pro Glu Gly Ala Val Glu Ala Glu Leu Pro Leu 290 295 300 Pro Gly Asp Phe Asn Ile Ala Asn Ala Ala Leu Ala Thr Ala Met Ala 305 310 315 320 Trp Ala Val Gly Val Asp Pro Gln Ala Phe Thr Ala Gly Phe Leu Asp 325 330 335 Val Ala Val Pro Gly Arg Met Glu Arg Ile Asp Glu Gly Gln Asp Phe 340 345 350 Leu Ala Val Val Asp Tyr Ala His Lys Pro Ala Ala Val Ala Ala Val 355 360 365 Leu Asp Thr Leu Arg Gly Gln Val Arg Gly Arg Leu Gly Val Val Val 370 375 380 Gly Ala Gly Gly Asp Arg Asp Ala Thr Lys Arg Pro Leu Met Gly Ala 385 390 395 400 Glu Ala Ala His Arg Ala Asp Leu Val Ile Ile Thr Asp Asp Asn Pro 405 410 415 Arg Ser Glu Val Pro Ala Thr Ile Arg Ala Ala Val Leu Gln Gly Ala 420 425 430 Val Asp Ala Gly Thr Asp Ala Glu Ile Arg Glu Ile Gly Asp Arg Ala 435 440 445 Ala Ala Ile Asp Ala Leu Ile Glu Trp Ala Arg Pro Gly Asp Gly Val 450 455 460 Val Val Ala Gly Lys Gly His Glu Val Gly Gln Leu Val Asn Gly Val 465 470 475 480 Asn His His Phe Asp Asp Arg Glu Glu Val Arg Arg Ala Leu Lys Glu 485 490 495 Lys Lys His <210> 185 <211> 2145 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1845) <223> gene zwf <400> 185 cgaccctcta cgtcaccgag ctggccggcc cggagaccgt caacaccatg ccggagccga 60 ccatcgacgc cgtgctcgag cagggtggcc tccacggcga caccctgagc ggcaccgcgg 120 acgacgcgca ggccgtcttc gagcagctgg tgcaggtcgg catcgacatg gaggacgtct 180 tcgcggtgct ggagcgcgag ggcgtcgaga agttcgtgtc ctcctggtcc gagctgctcg 240 actccatgga gtcccgactg cgctagcagt cccctccccg acaggaaagg acctgagcgc 300 atg ccc ctc ccc gtg acc ggc acc gac gcc tgg atc aac ccg ctg cgg 348 Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg 1 5 10 15 aac acc gtc gac aag cgg ctg ccc cgc atc gcc ggc ccc tcc ggc atg 396 Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met 20 25 30 gtg atc ttc ggc gtg acc ggt gac ctg gcc cgc aag aag ctg ctc ccg 444 Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro 35 40 45 gcc atc tac gac ctg gcc aac cgt ggc ctc ctg ccg gcc ggt ttc acc 492 Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr 50 55 60 ctc atc ggt ttc ggc cgt cgt ccc tgg agc aaa ccc gag ttc gag gag 540 Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu 65 70 75 80 tac gtg cgg gag gcc gtc gag agc ggc gcc cgc acc gag ttc cgc gaa 588 Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu 85 90 95 cac gtg tgg gag cgg ctg gcc gag ggc atg cac ttc gtc gag ggc aac 636 His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn 100 105 110 ttc gac gac gag ggc ttc gac cgc ctc gcc gcc acc ctc gcc gag atg 684 Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met 115 120 125 gac acc tcc cgc ggc acg gcg ggc aac tgg gcc ttc tac ctg tcg gtc 732 Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val 130 135 140 ccg ccg gac ttc ttc acc gcc gtc tgc cac cag ctg gag cgc tcc ggc 780 Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly 145 150 155 160 ctg gcg gag gcc ggt gag gag tcg tgg cgt cgc gtg atc atc gag aag 828 Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys 165 170 175 ccc ttc ggc cac gac cag gac acg gcc cgg gag ctc aac cgg gtg gtc 876 Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val 180 185 190 aac gcc gtc ttc ccg gag gag tcg gtg ttc cgc atc gac cac tac ctg 924 Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu 195 200 205 ggc aag gag acg gtg cag aac atc ctg gcg ctg cgc ttc gcc aac cag 972 Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln 210 215 220 ctc ttc gac ccg atc tgg aac gcg cac tac gtc gac cac gtg cag atc 1020 Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile 225 230 235 240 acc atg gcc gag gac atc ggt ctc ggt ggt cgg gcc ggc tac tac gac 1068 Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp 245 250 255 ggc atc ggc gcc gcc cgc gac gtc atg cag aac cac ctc ctc cag ctc 1116 Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu 260 265 270 ctg gcg ctg gtg gcc atg gag gag ccc atc gcc ttc gac ccg gct gag 1164 Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu 275 280 285 ctg cag gcg gag aag gtc aag gtc ctg cgc gcg acc aac ccc gtc ttc 1212 Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe 290 295 300 ccg ctg aac aag acc acc gcc cgt ggc cag tac gcg gcg ggc tgg cag 1260 Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln 305 310 315 320 ggc tcg cac aag gtg cgt ggc ctg cgt gag gag gag ggt ttc gac ccg 1308 Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro 325 330 335 gac tcc acc acg gag acc tac gcc gcc tgc acc ctg gag atc aac tcc 1356 Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser 340 345 350 cgc cgc tgg gcg ggc gtg ccc ttc tac ctg cgg acc ggc aag cgc ctg 1404 Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu 355 360 365 ggg cgc cgc gtc acg gag atc gcc ctc gtg ttc aag gag gcg ccg cac 1452 Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His 370 375 380 cag ccc ttc ctc gag ggg cag acg tcc tcg ctg ggg cag aac gcg atc 1500 Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile 385 390 395 400 gtc atc cgc gtc cag ccc gac gag ggc gtg ctc atg cgc ttc ggc tcc 1548 Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser 405 410 415 aag gtg ccc ggc tcc gcg atg gag gtc cgt gac gtc aac atg gac ttc 1596 Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe 420 425 430 tcc tac gcg gag gcc ttc acc gag gcg tcc ccg gag gcc tat gag cgg 1644 Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg 435 440 445 ctc atc ctc gat gcg ctt ctc gac gag tcc tcg ctc ttc ccc acc aac 1692 Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn 450 455 460 gag gag gtc gag ctc tcc tgg gcg atc ctg gac ccg atc ctc gag ttc 1740 Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe 465 470 475 480 tgg gct gag aag ggc cag ccc gag gag tac gag tcc ggc acc tgg ggg 1788 Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly 485 490 495 ccc gcc tcc gcc gac cgc atg ctg gaa cgg acc ggc cgc gcc tgg cgc 1836 Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg 500 505 510 cgc ccc tga cctggaaggg acccgacttg aagatcgaac tgccggacac 1885 Arg Pro cgacacccgg aacatcgccg ccagcctgct cgaggcccgg gagcacaact cccagaccac 1945 cgggcgggtg ctcaccctca tcgtcgtcgc cgcgctcgac gacgacgtcg accacatcat 2005 ctccgtcacc cgcgacgcct cccacgagca ccccgcccgt gtgctggtgc tgctcaccgg 2065 ccgggaggac gctgactcgc gtctcgacgc cgaggtgcgc gtcggcggtc acgccggcgc 2125 ctcggagatg gtcatcatgc 2145 <210> 186 <211> 514 <212> PRT <213> Corynebacterium humireducens <400> 186 Met Pro Leu Pro Val Thr Gly Thr Asp Ala Trp Ile Asn Pro Leu Arg 1 5 10 15 Asn Thr Val Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met 20 25 30 Val Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro 35 40 45 Ala Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Ala Gly Phe Thr 50 55 60 Leu Ile Gly Phe Gly Arg Arg Pro Trp Ser Lys Pro Glu Phe Glu Glu 65 70 75 80 Tyr Val Arg Glu Ala Val Glu Ser Gly Ala Arg Thr Glu Phe Arg Glu 85 90 95 His Val Trp Glu Arg Leu Ala Glu Gly Met His Phe Val Glu Gly Asn 100 105 110 Phe Asp Asp Glu Gly Phe Asp Arg Leu Ala Ala Thr Leu Ala Glu Met 115 120 125 Asp Thr Ser Arg Gly Thr Ala Gly Asn Trp Ala Phe Tyr Leu Ser Val 130 135 140 Pro Pro Asp Phe Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly 145 150 155 160 Leu Ala Glu Ala Gly Glu Glu Ser Trp Arg Arg Val Ile Ile Glu Lys 165 170 175 Pro Phe Gly His Asp Gln Asp Thr Ala Arg Glu Leu Asn Arg Val Val 180 185 190 Asn Ala Val Phe Pro Glu Glu Ser Val Phe Arg Ile Asp His Tyr Leu 195 200 205 Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln 210 215 220 Leu Phe Asp Pro Ile Trp Asn Ala His Tyr Val Asp His Val Gln Ile 225 230 235 240 Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp 245 250 255 Gly Ile Gly Ala Ala Arg Asp Val Met Gln Asn His Leu Leu Gln Leu 260 265 270 Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ala Phe Asp Pro Ala Glu 275 280 285 Leu Gln Ala Glu Lys Val Lys Val Leu Arg Ala Thr Asn Pro Val Phe 290 295 300 Pro Leu Asn Lys Thr Thr Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln 305 310 315 320 Gly Ser His Lys Val Arg Gly Leu Arg Glu Glu Glu Gly Phe Asp Pro 325 330 335 Asp Ser Thr Thr Glu Thr Tyr Ala Ala Cys Thr Leu Glu Ile Asn Ser 340 345 350 Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu 355 360 365 Gly Arg Arg Val Thr Glu Ile Ala Leu Val Phe Lys Glu Ala Pro His 370 375 380 Gln Pro Phe Leu Glu Gly Gln Thr Ser Ser Leu Gly Gln Asn Ala Ile 385 390 395 400 Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Met Arg Phe Gly Ser 405 410 415 Lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe 420 425 430 Ser Tyr Ala Glu Ala Phe Thr Glu Ala Ser Pro Glu Ala Tyr Glu Arg 435 440 445 Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn 450 455 460 Glu Glu Val Glu Leu Ser Trp Ala Ile Leu Asp Pro Ile Leu Glu Phe 465 470 475 480 Trp Ala Glu Lys Gly Gln Pro Glu Glu Tyr Glu Ser Gly Thr Trp Gly 485 490 495 Pro Ala Ser Ala Asp Arg Met Leu Glu Arg Thr Gly Arg Ala Trp Arg 500 505 510 Arg Pro <210> 187 <211> 1551 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1251) <223> gene opcA <400> 187 cgcgatggag gtccgtgacg tcaacatgga cttctcctac gcggaggcct tcaccgaggc 60 gtccccggag gcctatgagc ggctcatcct cgatgcgctt ctcgacgagt cctcgctctt 120 ccccaccaac gaggaggtcg agctctcctg ggcgatcctg gacccgatcc tcgagttctg 180 ggctgagaag ggccagcccg aggagtacga gtccggcacc tgggggcccg cctccgccga 240 ccgcatgctg gaacggaccg gccgcgcctg gcgccgcccc tgacctggaa gggacccgac 300 ttg aag atc gaa ctg ccg gac acc gac acc cgg aac atc gcc gcc agc 348 Leu Lys Ile Glu Leu Pro Asp Thr Asp Thr Arg Asn Ile Ala Ala Ser 1 5 10 15 ctg ctc gag gcc cgg gag cac aac tcc cag acc acc ggg cgg gtg ctc 396 Leu Leu Glu Ala Arg Glu His Asn Ser Gln Thr Thr Gly Arg Val Leu 20 25 30 acc ctc atc gtc gtc gcc gcg ctc gac gac gac gtc gac cac atc atc 444 Thr Leu Ile Val Val Ala Ala Leu Asp Asp Asp Val Asp His Ile Ile 35 40 45 tcc gtc acc cgc gac gcc tcc cac gag cac ccc gcc cgt gtg ctg gtg 492 Ser Val Thr Arg Asp Ala Ser His Glu His Pro Ala Arg Val Leu Val 50 55 60 ctg ctc acc ggc cgg gag gac gct gac tcg cgt ctc gac gcc gag gtg 540 Leu Leu Thr Gly Arg Glu Asp Ala Asp Ser Arg Leu Asp Ala Glu Val 65 70 75 80 cgc gtc ggc ggt cac gcc ggc gcc tcg gag atg gtc atc atg cgc atc 588 Arg Val Gly Gly His Ala Gly Ala Ser Glu Met Val Ile Met Arg Ile 85 90 95 acg ggc gag atg gtc aac cac ctc gcc gcg gtg gtc acc ccc ctg ctg 636 Thr Gly Glu Met Val Asn His Leu Ala Ala Val Val Thr Pro Leu Leu 100 105 110 ctg ccg gac acc ccg gtc gtc gcc tgg tgg ccg acc acc gcg ccg gag 684 Leu Pro Asp Thr Pro Val Val Ala Trp Trp Pro Thr Thr Ala Pro Glu 115 120 125 aac ccc tcc ggg cat ccg ctg ggc cag ctg gcg cag cgc cgc atc acc 732 Asn Pro Ser Gly His Pro Leu Gly Gln Leu Ala Gln Arg Arg Ile Thr 130 135 140 aac gcg gac cac ccg gac agt gag tcg gtc ctc cgg ctc cgc cgt gcc 780 Asn Ala Asp His Pro Asp Ser Glu Ser Val Leu Arg Leu Arg Arg Ala 145 150 155 160 acc tac gcc ccc ggt gac tcc gac atg gtg tgg tcg aag atc acc gcc 828 Thr Tyr Ala Pro Gly Asp Ser Asp Met Val Trp Ser Lys Ile Thr Ala 165 170 175 tgg cgc ggt atc gtc gcc tcc tcc ctg gac cgt cac ccg cac gag gac 876 Trp Arg Gly Ile Val Ala Ser Ser Leu Asp Arg His Pro His Glu Asp 180 185 190 gtg gag gcc gcc gtc ctc acc ggc ccg gcg gat gag ccg cag atc gac 924 Val Glu Ala Ala Val Leu Thr Gly Pro Ala Asp Glu Pro Gln Ile Asp 195 200 205 atc gcc gcc ggc tgg ctc gcc gac cgc ctc ggt gtg ccg gtc cgc cgg 972 Ile Ala Ala Gly Trp Leu Ala Asp Arg Leu Gly Val Pro Val Arg Arg 210 215 220 gag gtg tgt gaa ccg ggt aac gac gcc cgg ctc ttc ccc atc cgt cgt 1020 Glu Val Cys Glu Pro Gly Asn Asp Ala Arg Leu Phe Pro Ile Arg Arg 225 230 235 240 ctc aag ttc gaa cgc gcc tcc ggc ccg gtc cgc gtc gag gtg ctc gac 1068 Leu Lys Phe Glu Arg Ala Ser Gly Pro Val Arg Val Glu Val Leu Asp 245 250 255 cac cgg acc gtg ctc gtc gcc ctg cca ggg cgc ccg gag tcg ctc gtc 1116 His Arg Thr Val Leu Val Ala Leu Pro Gly Arg Pro Glu Ser Leu Val 260 265 270 gcc atg aac gag cgt tcc gac gcc gac tgc ctc tcc gag gaa ctg cgt 1164 Ala Met Asn Glu Arg Ser Asp Ala Asp Cys Leu Ser Glu Glu Leu Arg 275 280 285 cac ctc gac ccc gat ctc gcc tac gcc aac gct ctg cgc gga ctc acc 1212 His Leu Asp Pro Asp Leu Ala Tyr Ala Asn Ala Leu Arg Gly Leu Thr 290 295 300 cgt gtg aaa gga atc aat ggt cac cct gcg tcg tcc tga caccctgtcc 1261 Arg Val Lys Gly Ile Asn Gly His Pro Ala Ser Ser 305 310 315 accctgctcg accaggccgc cacgtcgctc atcgacgtcg tcgctgccct ccagaccacc 1321 gggggcggtg tccacgccga cggtgtggcc cgcctggtgc tcaccggcgg cggcgccggc 1381 atcggcctgc tggagcgcgt ccgtgagctc gaggacgccg actcccgcat cgactggtcc 1441 cgcgtccacg tcttcttcgg cgacgagcgc aacgtgcccg tctcccacga ggactccaac 1501 gaggggcagg cgcgtaccgc cctgctcagc cacgtcgaca tcccggaggc 1551 <210> 188 <211> 316 <212> PRT <213> Corynebacterium humireducens <400> 188 Leu Lys Ile Glu Leu Pro Asp Thr Asp Thr Arg Asn Ile Ala Ala Ser 1 5 10 15 Leu Leu Glu Ala Arg Glu His Asn Ser Gln Thr Thr Gly Arg Val Leu 20 25 30 Thr Leu Ile Val Val Ala Ala Leu Asp Asp Asp Val Asp His Ile Ile 35 40 45 Ser Val Thr Arg Asp Ala Ser His Glu His Pro Ala Arg Val Leu Val 50 55 60 Leu Leu Thr Gly Arg Glu Asp Ala Asp Ser Arg Leu Asp Ala Glu Val 65 70 75 80 Arg Val Gly Gly His Ala Gly Ala Ser Glu Met Val Ile Met Arg Ile 85 90 95 Thr Gly Glu Met Val Asn His Leu Ala Ala Val Val Thr Pro Leu Leu 100 105 110 Leu Pro Asp Thr Pro Val Val Ala Trp Trp Pro Thr Thr Ala Pro Glu 115 120 125 Asn Pro Ser Gly His Pro Leu Gly Gln Leu Ala Gln Arg Arg Ile Thr 130 135 140 Asn Ala Asp His Pro Asp Ser Glu Ser Val Leu Arg Leu Arg Arg Ala 145 150 155 160 Thr Tyr Ala Pro Gly Asp Ser Asp Met Val Trp Ser Lys Ile Thr Ala 165 170 175 Trp Arg Gly Ile Val Ala Ser Ser Leu Asp Arg His Pro His Glu Asp 180 185 190 Val Glu Ala Ala Val Leu Thr Gly Pro Ala Asp Glu Pro Gln Ile Asp 195 200 205 Ile Ala Ala Gly Trp Leu Ala Asp Arg Leu Gly Val Pro Val Arg Arg 210 215 220 Glu Val Cys Glu Pro Gly Asn Asp Ala Arg Leu Phe Pro Ile Arg Arg 225 230 235 240 Leu Lys Phe Glu Arg Ala Ser Gly Pro Val Arg Val Glu Val Leu Asp 245 250 255 His Arg Thr Val Leu Val Ala Leu Pro Gly Arg Pro Glu Ser Leu Val 260 265 270 Ala Met Asn Glu Arg Ser Asp Ala Asp Cys Leu Ser Glu Glu Leu Arg 275 280 285 His Leu Asp Pro Asp Leu Ala Tyr Ala Asn Ala Leu Arg Gly Leu Thr 290 295 300 Arg Val Lys Gly Ile Asn Gly His Pro Ala Ser Ser 305 310 315 <210> 189 <211> 2283 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1983) <223> gene hutU (Urocanate Hydratase) <400> 189 gctacctgtc cccggagatc gaggagtcgg tgcagctgct cacctccggg gcgctgctgg 60 aggcggtgga gggggtcgtc ggaaagctgg catgagcggg cgaatctcag atatgagact 120 aaaggggcct ccgggccctc gatcccgact gtgacctgtc ttaccgtcag aagtgtcacc 180 gacaccggcc acaccagccc ggtgctccac acccgacgat gacagccccg ggccacccgg 240 atcgcgggtg ccccggggcc ccggcctgat cggcccccac cgtagaagga gacccacccc 300 atg acc gag tcc ccg cgc acc atc cgt gcc ccg cac ggc acc gag ctc 348 Met Thr Glu Ser Pro Arg Thr Ile Arg Ala Pro His Gly Thr Glu Leu 1 5 10 15 agc gcc aag agc tgg caa acc gag gca ccc ctg cgc atg ctc atg aac 396 Ser Ala Lys Ser Trp Gln Thr Glu Ala Pro Leu Arg Met Leu Met Asn 20 25 30 aac ctc gac ccg gag gtc gcc gag cgc ccc gag gac ctc gtc gtc tac 444 Asn Leu Asp Pro Glu Val Ala Glu Arg Pro Glu Asp Leu Val Val Tyr 35 40 45 ggc ggc acc ggc cgc gcg gca cgc agc tgg gag gcc tac gac gcg atc 492 Gly Gly Thr Gly Arg Ala Ala Arg Ser Trp Glu Ala Tyr Asp Ala Ile 50 55 60 atc gag acg ctc agg gac ctc gag gag gac gag acc ctg ctc gtc cag 540 Ile Glu Thr Leu Arg Asp Leu Glu Glu Asp Glu Thr Leu Leu Val Gln 65 70 75 80 tcc ggc aag ccc gtc ggc gtc ttc cgc acc aac gtc tgg gca ccc cgc 588 Ser Gly Lys Pro Val Gly Val Phe Arg Thr Asn Val Trp Ala Pro Arg 85 90 95 gtg ctc atc gcc aac tcc aac ctc gtc ggc gac tgg gcc aac tgg ccc 636 Val Leu Ile Ala Asn Ser Asn Leu Val Gly Asp Trp Ala Asn Trp Pro 100 105 110 gag ttc cgc cgc ctc gag gcc gag ggc ctc atg atg tac ggc cag atg 684 Glu Phe Arg Arg Leu Glu Ala Glu Gly Leu Met Met Tyr Gly Gln Met 115 120 125 acc gcc ggt tcc tgg atc tac atc gcc acc cag ggc atc ctc cag ggc 732 Thr Ala Gly Ser Trp Ile Tyr Ile Ala Thr Gln Gly Ile Leu Gln Gly 130 135 140 acc tac gag acc ttc gcc gcc gtg gcc cgc aag cgt ttc ggc ggc acc 780 Thr Tyr Glu Thr Phe Ala Ala Val Ala Arg Lys Arg Phe Gly Gly Thr 145 150 155 160 ctc gcc ggc acc ctc acc ctc acc ggc ggc tgc ggc ggc atg ggc ggg 828 Leu Ala Gly Thr Leu Thr Leu Thr Gly Gly Cys Gly Gly Met Gly Gly 165 170 175 gcc cag ccg ctg tcc gtc acc ctc aac ggc ggt gtc tgc ctc atc gtc 876 Ala Gln Pro Leu Ser Val Thr Leu Asn Gly Gly Val Cys Leu Ile Val 180 185 190 gac gtc gac gag tcc cgg ctc agg cgt cgg cag gcc aag cgc tac ctc 924 Asp Val Asp Glu Ser Arg Leu Arg Arg Arg Gln Ala Lys Arg Tyr Leu 195 200 205 gat gag gtc gcc acc gat ctc gac gac gcc gtc gcc cgc gtc agc gcc 972 Asp Glu Val Ala Thr Asp Leu Asp Asp Ala Val Ala Arg Val Ser Ala 210 215 220 gcc aag gag gag aag cgt gcc ctg tcc gtc ggc cta gtg ggc aat gcc 1020 Ala Lys Glu Glu Lys Arg Ala Leu Ser Val Gly Leu Val Gly Asn Ala 225 230 235 240 gcc gag gtg ttc ccc gag ctg ctg cgc cgc cac cgc gcc ggg gag atc 1068 Ala Glu Val Phe Pro Glu Leu Leu Arg Arg His Arg Ala Gly Glu Ile 245 250 255 acc gtc gac gtg gtc acc gac cag acc tcc gcc cac gac ccg ctc agc 1116 Thr Val Asp Val Val Thr Asp Gln Thr Ser Ala His Asp Pro Leu Ser 260 265 270 tac ctg ccc acc gag atc acc gtc gag gac tgg cag cgc gag gcc gcc 1164 Tyr Leu Pro Thr Glu Ile Thr Val Glu Asp Trp Gln Arg Glu Ala Ala 275 280 285 gag gac ccc atc acc ttc acc aag aag gca cgc gag tcc atg gcc gtg 1212 Glu Asp Pro Ile Thr Phe Thr Lys Lys Ala Arg Glu Ser Met Ala Val 290 295 300 cag gtc cag gcc atg gtc gag ttc cag gac gag ggc gcc gag gtc ttc 1260 Gln Val Gln Ala Met Val Glu Phe Gln Asp Glu Gly Ala Glu Val Phe 305 310 315 320 gac tac ggc aac tcc atc cgc gat gag gcc cgc cac gcc ggc tac tcc 1308 Asp Tyr Gly Asn Ser Ile Arg Asp Glu Ala Arg His Ala Gly Tyr Ser 325 330 335 cgc gcc ttc gag ttc ccc ggc ttc gtc ccc gcc tac atc cgg ccc ctg 1356 Arg Ala Phe Glu Phe Pro Gly Phe Val Pro Ala Tyr Ile Arg Pro Leu 340 345 350 ttc tgc gag ggt ctc ggc ccc ttc cgc tgg gtg gcc ctt tcc ggc gac 1404 Phe Cys Glu Gly Leu Gly Pro Phe Arg Trp Val Ala Leu Ser Gly Asp 355 360 365 ccg gag gac atc cgc gtc acc gac gag gcc gtc aag gag ctg ttc ccc 1452 Pro Glu Asp Ile Arg Val Thr Asp Glu Ala Val Lys Glu Leu Phe Pro 370 375 380 gac aac gag cac ctc cac cgc tgg atc gac gcg gca ggg gag tac gtc 1500 Asp Asn Glu His Leu His Arg Trp Ile Asp Ala Ala Gly Glu Tyr Val 385 390 395 400 gag ttc gag ggc ctg ccg gcc cgc atc tgc tgg ctc ggc tac ggc gag 1548 Glu Phe Glu Gly Leu Pro Ala Arg Ile Cys Trp Leu Gly Tyr Gly Glu 405 410 415 cgg cac aag gcg gga ctg ctg ttc aac cgt ctc gtc gcc gag ggc aag 1596 Arg His Lys Ala Gly Leu Leu Phe Asn Arg Leu Val Ala Glu Gly Lys 420 425 430 gtg aag gcg ccc atc gtc atc ggc cgt gac cac ctc gac tcc ggt tcc 1644 Val Lys Ala Pro Ile Val Ile Gly Arg Asp His Leu Asp Ser Gly Ser 435 440 445 gtc gcc tcc ccc tac cgg gag acc gag tcc atg ctc gac ggc tcc gac 1692 Val Ala Ser Pro Tyr Arg Glu Thr Glu Ser Met Leu Asp Gly Ser Asp 450 455 460 gcc atc gcc gac tgg ccg ctg ctc aac gcc ctc acc gcc acc tcc tcc 1740 Ala Ile Ala Asp Trp Pro Leu Leu Asn Ala Leu Thr Ala Thr Ser Ser 465 470 475 480 ggc gcg acc tgg gtc tcc ctg cac cac gga ggc ggc gtg ggc atc gga 1788 Gly Ala Thr Trp Val Ser Leu His His Gly Gly Gly Val Gly Ile Gly 485 490 495 cgg tcc atc cac gcg ggc cag gtg tgc gtc gcc gac ggc acg gag ctg 1836 Arg Ser Ile His Ala Gly Gln Val Cys Val Ala Asp Gly Thr Glu Leu 500 505 510 gcc gcc gcg aag ctc acc gcc gtg ctc acc aac gac ccg ggc atg ggc 1884 Ala Ala Ala Lys Leu Thr Ala Val Leu Thr Asn Asp Pro Gly Met Gly 515 520 525 gtc atc cgc cac gtc gac gcc ggc tac gac cgt gcc cac gag gtc gcc 1932 Val Ile Arg His Val Asp Ala Gly Tyr Asp Arg Ala His Glu Val Ala 530 535 540 gtc gag cgc ggg gtc cgt gtc ccc atg ccg ttc gtc agc cgc gag agg 1980 Val Glu Arg Gly Val Arg Val Pro Met Pro Phe Val Ser Arg Glu Arg 545 550 555 560 tag ccatgagcac gctgttccac ggaatctccg agctgaggac cgtctccggg 2033 ctgggcacgc tcagcgacgc cgcgctcatc gtcgaccggg gccgcatcgc ctggatcggc 2093 ccgcgcgccg gggcccccgc cgccgacgcg gcggttgatc tgggcggccg ggccgtgcta 2153 cccggctggg tggactccca cacccacatg atcttcgcgg gggaccgcgc cgccgagttc 2213 gaggcccgca tgaacgggca ggactaccag gcgggcggca tcgcggtgac catgtccgcc 2273 acccgcgagg 2283 <210> 190 <211> 560 <212> PRT <213> Corynebacterium humireducens <400> 190 Met Thr Glu Ser Pro Arg Thr Ile Arg Ala Pro His Gly Thr Glu Leu 1 5 10 15 Ser Ala Lys Ser Trp Gln Thr Glu Ala Pro Leu Arg Met Leu Met Asn 20 25 30 Asn Leu Asp Pro Glu Val Ala Glu Arg Pro Glu Asp Leu Val Val Tyr 35 40 45 Gly Gly Thr Gly Arg Ala Ala Arg Ser Trp Glu Ala Tyr Asp Ala Ile 50 55 60 Ile Glu Thr Leu Arg Asp Leu Glu Glu Asp Glu Thr Leu Leu Val Gln 65 70 75 80 Ser Gly Lys Pro Val Gly Val Phe Arg Thr Asn Val Trp Ala Pro Arg 85 90 95 Val Leu Ile Ala Asn Ser Asn Leu Val Gly Asp Trp Ala Asn Trp Pro 100 105 110 Glu Phe Arg Arg Leu Glu Ala Glu Gly Leu Met Met Tyr Gly Gln Met 115 120 125 Thr Ala Gly Ser Trp Ile Tyr Ile Ala Thr Gln Gly Ile Leu Gln Gly 130 135 140 Thr Tyr Glu Thr Phe Ala Ala Val Ala Arg Lys Arg Phe Gly Gly Thr 145 150 155 160 Leu Ala Gly Thr Leu Thr Leu Thr Gly Gly Cys Gly Gly Met Gly Gly 165 170 175 Ala Gln Pro Leu Ser Val Thr Leu Asn Gly Gly Val Cys Leu Ile Val 180 185 190 Asp Val Asp Glu Ser Arg Leu Arg Arg Arg Gln Ala Lys Arg Tyr Leu 195 200 205 Asp Glu Val Ala Thr Asp Leu Asp Asp Ala Val Ala Arg Val Ser Ala 210 215 220 Ala Lys Glu Glu Lys Arg Ala Leu Ser Val Gly Leu Val Gly Asn Ala 225 230 235 240 Ala Glu Val Phe Pro Glu Leu Leu Arg Arg His Arg Ala Gly Glu Ile 245 250 255 Thr Val Asp Val Val Thr Asp Gln Thr Ser Ala His Asp Pro Leu Ser 260 265 270 Tyr Leu Pro Thr Glu Ile Thr Val Glu Asp Trp Gln Arg Glu Ala Ala 275 280 285 Glu Asp Pro Ile Thr Phe Thr Lys Lys Ala Arg Glu Ser Met Ala Val 290 295 300 Gln Val Gln Ala Met Val Glu Phe Gln Asp Glu Gly Ala Glu Val Phe 305 310 315 320 Asp Tyr Gly Asn Ser Ile Arg Asp Glu Ala Arg His Ala Gly Tyr Ser 325 330 335 Arg Ala Phe Glu Phe Pro Gly Phe Val Pro Ala Tyr Ile Arg Pro Leu 340 345 350 Phe Cys Glu Gly Leu Gly Pro Phe Arg Trp Val Ala Leu Ser Gly Asp 355 360 365 Pro Glu Asp Ile Arg Val Thr Asp Glu Ala Val Lys Glu Leu Phe Pro 370 375 380 Asp Asn Glu His Leu His Arg Trp Ile Asp Ala Ala Gly Glu Tyr Val 385 390 395 400 Glu Phe Glu Gly Leu Pro Ala Arg Ile Cys Trp Leu Gly Tyr Gly Glu 405 410 415 Arg His Lys Ala Gly Leu Leu Phe Asn Arg Leu Val Ala Glu Gly Lys 420 425 430 Val Lys Ala Pro Ile Val Ile Gly Arg Asp His Leu Asp Ser Gly Ser 435 440 445 Val Ala Ser Pro Tyr Arg Glu Thr Glu Ser Met Leu Asp Gly Ser Asp 450 455 460 Ala Ile Ala Asp Trp Pro Leu Leu Asn Ala Leu Thr Ala Thr Ser Ser 465 470 475 480 Gly Ala Thr Trp Val Ser Leu His His Gly Gly Gly Val Gly Ile Gly 485 490 495 Arg Ser Ile His Ala Gly Gln Val Cys Val Ala Asp Gly Thr Glu Leu 500 505 510 Ala Ala Ala Lys Leu Thr Ala Val Leu Thr Asn Asp Pro Gly Met Gly 515 520 525 Val Ile Arg His Val Asp Ala Gly Tyr Asp Arg Ala His Glu Val Ala 530 535 540 Val Glu Arg Gly Val Arg Val Pro Met Pro Phe Val Ser Arg Glu Arg 545 550 555 560 <210> 191 <211> 1809 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1509) <223> gene hutI (Imidazolone Propionase) <400> 191 ctccgacgcc atcgccgact ggccgctgct caacgccctc accgccacct cctccggcgc 60 gacctgggtc tccctgcacc acggaggcgg cgtgggcatc ggacggtcca tccacgcggg 120 ccaggtgtgc gtcgccgacg gcacggagct ggccgccgcg aagctcaccg ccgtgctcac 180 caacgacccg ggcatgggcg tcatccgcca cgtcgacgcc ggctacgacc gtgcccacga 240 ggtcgccgtc gagcgcgggg tccgtgtccc catgccgttc gtcagccgcg agaggtagcc 300 atg agc acg ctg ttc cac gga atc tcc gag ctg agg acc gtc tcc ggg 348 Met Ser Thr Leu Phe His Gly Ile Ser Glu Leu Arg Thr Val Ser Gly 1 5 10 15 ctg ggc acg ctc agc gac gcc gcg ctc atc gtc gac cgg ggc cgc atc 396 Leu Gly Thr Leu Ser Asp Ala Ala Leu Ile Val Asp Arg Gly Arg Ile 20 25 30 gcc tgg atc ggc ccg cgc gcc ggg gcc ccc gcc gcc gac gcg gcg gtt 444 Ala Trp Ile Gly Pro Arg Ala Gly Ala Pro Ala Ala Asp Ala Ala Val 35 40 45 gat ctg ggc ggc cgg gcc gtg cta ccc ggc tgg gtg gac tcc cac acc 492 Asp Leu Gly Gly Arg Ala Val Leu Pro Gly Trp Val Asp Ser His Thr 50 55 60 cac atg atc ttc gcg ggg gac cgc gcc gcc gag ttc gag gcc cgc atg 540 His Met Ile Phe Ala Gly Asp Arg Ala Ala Glu Phe Glu Ala Arg Met 65 70 75 80 aac ggg cag gac tac cag gcg ggc ggc atc gcg gtg acc atg tcc gcc 588 Asn Gly Gln Asp Tyr Gln Ala Gly Gly Ile Ala Val Thr Met Ser Ala 85 90 95 acc cgc gag gcc ggc gcc gtc cgc ctc gag cag ctg ctg ctg cgc cgc 636 Thr Arg Glu Ala Gly Ala Val Arg Leu Glu Gln Leu Leu Leu Arg Arg 100 105 110 ctg gcc gat gcg cgc gcc ggg ggc acg acc acg gtg gag acg aag acc 684 Leu Ala Asp Ala Arg Ala Gly Gly Thr Thr Thr Val Glu Thr Lys Thr 115 120 125 ggc tac ggg ctc gac gtg gac tcc gag gcc ctg gcg gcc gac atc gcc 732 Gly Tyr Gly Leu Asp Val Asp Ser Glu Ala Leu Ala Ala Asp Ile Ala 130 135 140 gcc cgg cac gtc gat gac gtc acc ttc ctc ggc gcg cat ctc gtg ccg 780 Ala Arg His Val Asp Asp Val Thr Phe Leu Gly Ala His Leu Val Pro 145 150 155 160 ccg ggg gag gac ccg gac gag tac ctc gct ctc gtg ctc ggc gac atg 828 Pro Gly Glu Asp Pro Asp Glu Tyr Leu Ala Leu Val Leu Gly Asp Met 165 170 175 ctc gac gcc gtc gcg gcc aac gtc agc tgg atc gac gtg ttc tgc gaa 876 Leu Asp Ala Val Ala Ala Asn Val Ser Trp Ile Asp Val Phe Cys Glu 180 185 190 cgc ggg gcc ttc gat gag gcg gcc gcc cgc cgc gtc ctc gag gcc ggg 924 Arg Gly Ala Phe Asp Glu Ala Ala Ala Arg Arg Val Leu Glu Ala Gly 195 200 205 cgg gcg cgg ggt ctg ggg ctg cgc gtg cac ggc aac cag ctc ggg cac 972 Arg Ala Arg Gly Leu Gly Leu Arg Val His Gly Asn Gln Leu Gly His 210 215 220 ggt ccc gga gtg cag ctg gcg gtg gag tac ggc gcc gcc agc gtg gac 1020 Gly Pro Gly Val Gln Leu Ala Val Glu Tyr Gly Ala Ala Ser Val Asp 225 230 235 240 cac gtc aac tac gtg agt gac ggt gac gtg gag gca ctc gcc tcc tcg 1068 His Val Asn Tyr Val Ser Asp Gly Asp Val Glu Ala Leu Ala Ser Ser 245 250 255 gag acg gtg gcc acg gtc ctc ccc gcc tgc gac ctg tcc acg agg cag 1116 Glu Thr Val Ala Thr Val Leu Pro Ala Cys Asp Leu Ser Thr Arg Gln 260 265 270 ccg ctg gcg ccg gcc cgg agg ctt ctc gac gcc ggg gtc cac ctc gcc 1164 Pro Leu Ala Pro Ala Arg Arg Leu Leu Asp Ala Gly Val His Leu Ala 275 280 285 ctc gcc tcc aac ctc aac ccg ggc acg tcg tac acc tcg tcg atg gcc 1212 Leu Ala Ser Asn Leu Asn Pro Gly Thr Ser Tyr Thr Ser Ser Met Ala 290 295 300 ttc tgc gtc acc acc gcg gtg ctg cag cag cac ctc acc ctc gac gag 1260 Phe Cys Val Thr Thr Ala Val Leu Gln Gln His Leu Thr Leu Asp Glu 305 310 315 320 gcc atc cac gcg gcc acc gcc ggt ggg gcg cgg gcg ctg cgg cgt cac 1308 Ala Ile His Ala Ala Thr Ala Gly Gly Ala Arg Ala Leu Arg Arg His 325 330 335 gac atc ggc ggc ggc gtc gac ccc cag ggc cga ccc gcc cgc ggc agc 1356 Asp Ile Gly Gly Gly Val Asp Pro Gln Gly Arg Pro Ala Arg Gly Ser 340 345 350 ctc gtg gtc ggg gcc gcg gca gac ctg cac gtg ctg gac acc ccc cac 1404 Leu Val Val Gly Ala Ala Ala Asp Leu His Val Leu Asp Thr Pro His 355 360 365 gcc atc gat ctg gcc tac cgg ccg ggg atg ccg ctc acg tgg cgg acc 1452 Ala Ile Asp Leu Ala Tyr Arg Pro Gly Met Pro Leu Thr Trp Arg Thr 370 375 380 tac atc cag ggg gtg cag gtc cac ccg gaa ccg cag gat cag tcc gac 1500 Tyr Ile Gln Gly Val Gln Val His Pro Glu Pro Gln Asp Gln Ser Asp 385 390 395 400 ggc cga tga cgcacgatct cctccacgat ccgggccgcg acccgcgcgg 1549 Gly Arg tccggccgtc gacgtcgaga agcgggttga gttcgacgac atcgaccaga cgcagcttcc 1609 cgctgcgcgc cacggctctg acgagcgtca ggatccgtgc cagatcgacg cccaacgccg 1669 ccggcgcgct cacgccgggg gcgaccgccg cgggcaggac gtcaaggtcg atgctcaggt 1729 ggacgtccgg ggcgtcctcc aggagctttc cgacgagcgc ctcgcacccg gccgacgtca 1789 tctccgccag ctcgacgtcg 1809 <210> 192 <211> 402 <212> PRT <213> Corynebacterium humireducens <400> 192 Met Ser Thr Leu Phe His Gly Ile Ser Glu Leu Arg Thr Val Ser Gly 1 5 10 15 Leu Gly Thr Leu Ser Asp Ala Ala Leu Ile Val Asp Arg Gly Arg Ile 20 25 30 Ala Trp Ile Gly Pro Arg Ala Gly Ala Pro Ala Ala Asp Ala Ala Val 35 40 45 Asp Leu Gly Gly Arg Ala Val Leu Pro Gly Trp Val Asp Ser His Thr 50 55 60 His Met Ile Phe Ala Gly Asp Arg Ala Ala Glu Phe Glu Ala Arg Met 65 70 75 80 Asn Gly Gln Asp Tyr Gln Ala Gly Gly Ile Ala Val Thr Met Ser Ala 85 90 95 Thr Arg Glu Ala Gly Ala Val Arg Leu Glu Gln Leu Leu Leu Arg Arg 100 105 110 Leu Ala Asp Ala Arg Ala Gly Gly Thr Thr Thr Val Glu Thr Lys Thr 115 120 125 Gly Tyr Gly Leu Asp Val Asp Ser Glu Ala Leu Ala Ala Asp Ile Ala 130 135 140 Ala Arg His Val Asp Asp Val Thr Phe Leu Gly Ala His Leu Val Pro 145 150 155 160 Pro Gly Glu Asp Pro Asp Glu Tyr Leu Ala Leu Val Leu Gly Asp Met 165 170 175 Leu Asp Ala Val Ala Ala Asn Val Ser Trp Ile Asp Val Phe Cys Glu 180 185 190 Arg Gly Ala Phe Asp Glu Ala Ala Ala Arg Arg Val Leu Glu Ala Gly 195 200 205 Arg Ala Arg Gly Leu Gly Leu Arg Val His Gly Asn Gln Leu Gly His 210 215 220 Gly Pro Gly Val Gln Leu Ala Val Glu Tyr Gly Ala Ala Ser Val Asp 225 230 235 240 His Val Asn Tyr Val Ser Asp Gly Asp Val Glu Ala Leu Ala Ser Ser 245 250 255 Glu Thr Val Ala Thr Val Leu Pro Ala Cys Asp Leu Ser Thr Arg Gln 260 265 270 Pro Leu Ala Pro Ala Arg Arg Leu Leu Asp Ala Gly Val His Leu Ala 275 280 285 Leu Ala Ser Asn Leu Asn Pro Gly Thr Ser Tyr Thr Ser Ser Met Ala 290 295 300 Phe Cys Val Thr Thr Ala Val Leu Gln Gln His Leu Thr Leu Asp Glu 305 310 315 320 Ala Ile His Ala Ala Thr Ala Gly Gly Ala Arg Ala Leu Arg Arg His 325 330 335 Asp Ile Gly Gly Gly Val Asp Pro Gln Gly Arg Pro Ala Arg Gly Ser 340 345 350 Leu Val Val Gly Ala Ala Ala Asp Leu His Val Leu Asp Thr Pro His 355 360 365 Ala Ile Asp Leu Ala Tyr Arg Pro Gly Met Pro Leu Thr Trp Arg Thr 370 375 380 Tyr Ile Gln Gly Val Gln Val His Pro Glu Pro Gln Asp Gln Ser Asp 385 390 395 400 Gly Arg <210> 193 <211> 2151 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1851) <223> gene hutH (Histidine Ammonialyase) <400> 193 ccccggcgtc gtcggtaccg gcccggaatg ccatccgtgc agctcagagc cctgacgggc 60 atctcgtatg cgagatagat tccgcgcaca ccggaaagtc tcgtaagtga gacagccgtg 120 cccttcacgg gctgtcgccg gggcgtgacg gctgacacac tgggaggaac tgcgtccggc 180 tgtcgcgcag gtcgaaccgt ccacgagtca ccgccccgga ggacaggtac cacctgtgcc 240 tgccccgggg ccggtgcccc ggagaaaccg ggacccacca ggaagcaggg gaagccccac 300 atg cag acc atc acc tcc cac atc cgc gtc ggc gtc gga gcg ctc agc 348 Met Gln Thr Ile Thr Ser His Ile Arg Val Gly Val Gly Ala Leu Ser 1 5 10 15 atc gac gag gtc gtg gct gtc gcc cgg cac gac gcc cac gtc gag atc 396 Ile Asp Glu Val Val Ala Val Ala Arg His Asp Ala His Val Glu Ile 20 25 30 acc ccg gag gcg ctc gag gcc gtc gcc gcc acc cgt gcc cgc atc gag 444 Thr Pro Glu Ala Leu Glu Ala Val Ala Ala Thr Arg Ala Arg Ile Glu 35 40 45 gaa ctg gcc gac cac ccg acc ccg gtg tac ggc gtg tcc acc ggt ttc 492 Glu Leu Ala Asp His Pro Thr Pro Val Tyr Gly Val Ser Thr Gly Phe 50 55 60 ggc gcc ctg gcc cgc cgc tac atc ccc cag gag atg cgc ctc cag ctg 540 Gly Ala Leu Ala Arg Arg Tyr Ile Pro Gln Glu Met Arg Leu Gln Leu 65 70 75 80 cag cgc agc ctc gtc cgc tcc cat gcc gcc ggc tcc ggc ccc gag gtg 588 Gln Arg Ser Leu Val Arg Ser His Ala Ala Gly Ser Gly Pro Glu Val 85 90 95 gag cgc gag gtc gtc cgg gcg ctc atg ctg ctg cgc ctg tcc aca ctg 636 Glu Arg Glu Val Val Arg Ala Leu Met Leu Leu Arg Leu Ser Thr Leu 100 105 110 tgc acc ggc cgt acc ggc gtg cgg ccg gtc gtc gcg gag acc tac gcc 684 Cys Thr Gly Arg Thr Gly Val Arg Pro Val Val Ala Glu Thr Tyr Ala 115 120 125 gcg gtg ctc aac gcc ggc atc acc ccc gtc gtc cac gag tac ggc tcg 732 Ala Val Leu Asn Ala Gly Ile Thr Pro Val Val His Glu Tyr Gly Ser 130 135 140 ctc ggc tgc tcc ggc gac ctc tcc ccg ctg gcg cac tgc gcc ctg gcg 780 Leu Gly Cys Ser Gly Asp Leu Ser Pro Leu Ala His Cys Ala Leu Ala 145 150 155 160 ctg ctg ggg gag ggc gag gtc acc acc gct gac ggc cgc cgc ctg ccc 828 Leu Leu Gly Glu Gly Glu Val Thr Thr Ala Asp Gly Arg Arg Leu Pro 165 170 175 gcc gcc gtc gcc ctc gcc gag cac ggc atc gag ccg ctg gtc ctg cgg 876 Ala Ala Val Ala Leu Ala Glu His Gly Ile Glu Pro Leu Val Leu Arg 180 185 190 gag aag gag ggg ctg gca ctc atc aac ggc acc gac ggc atg ctg ggc 924 Glu Lys Glu Gly Leu Ala Leu Ile Asn Gly Thr Asp Gly Met Leu Gly 195 200 205 atg ctg tgc ctg gcc atc gct gac ctc gag tgc ctg gcc cgc agc gcc 972 Met Leu Cys Leu Ala Ile Ala Asp Leu Glu Cys Leu Ala Arg Ser Ala 210 215 220 gac atc gcc gcc gcg atg acc gtc gag gcc ctg cgg ggc acc ctc acc 1020 Asp Ile Ala Ala Ala Met Thr Val Glu Ala Leu Arg Gly Thr Leu Thr 225 230 235 240 gtc ttc gcc gcg gac ctg cag gag ctg cgc ccg cac ccg ggt cag gcg 1068 Val Phe Ala Ala Asp Leu Gln Glu Leu Arg Pro His Pro Gly Gln Ala 245 250 255 gcc tcc gcg gcc aac atc cgc gcc gtg gcc gag ggc tcc gcc ata ctg 1116 Ala Ser Ala Ala Asn Ile Arg Ala Val Ala Glu Gly Ser Ala Ile Leu 260 265 270 cag tcc gcc acc gag gaa ttc gcc ctg acc cac gtg cag gac gcc tac 1164 Gln Ser Ala Thr Glu Glu Phe Ala Leu Thr His Val Gln Asp Ala Tyr 275 280 285 tcg gtg cgg tgc gcc ccg cag gtc gcc ggt ggt ttc cgc gac acc ctg 1212 Ser Val Arg Cys Ala Pro Gln Val Ala Gly Gly Phe Arg Asp Thr Leu 290 295 300 gcc cac gcc cgc ctg gtc gcc gac cgg gag ctc gcc tcc gcc gtg gac 1260 Ala His Ala Arg Leu Val Ala Asp Arg Glu Leu Ala Ser Ala Val Asp 305 310 315 320 aac ccc gtg gtg gct ctc gac ggc cgc gtg acc tcc aac ggc aac ttc 1308 Asn Pro Val Val Ala Leu Asp Gly Arg Val Thr Ser Asn Gly Asn Phe 325 330 335 cac ggc gcg ccc gtc ggc tac gcg ctc gac ttc ctc gcc atc gtc acc 1356 His Gly Ala Pro Val Gly Tyr Ala Leu Asp Phe Leu Ala Ile Val Thr 340 345 350 gcc gac ctg gcg agc atc tcc gag cgc cgc acc gac cgg ctt ctc gac 1404 Ala Asp Leu Ala Ser Ile Ser Glu Arg Arg Thr Asp Arg Leu Leu Asp 355 360 365 gtc gcc cgc agc cgc gga ctc aac gcc ttc ctc gcc gac gat ccg ggc 1452 Val Ala Arg Ser Arg Gly Leu Asn Ala Phe Leu Ala Asp Asp Pro Gly 370 375 380 gtg gac tcc ggc cac atg atc gcc cag tac acg cag gcc ggg atc gtc 1500 Val Asp Ser Gly His Met Ile Ala Gln Tyr Thr Gln Ala Gly Ile Val 385 390 395 400 tcc gag ctc aag cgg ctg gcc gcc ccg gcc agc gtg gac tcc atc ccc 1548 Ser Glu Leu Lys Arg Leu Ala Ala Pro Ala Ser Val Asp Ser Ile Pro 405 410 415 tcc tcc gcc atg cag gag gac cac gtg tcc atg ggc tgg tcg gcc ggg 1596 Ser Ser Ala Met Gln Glu Asp His Val Ser Met Gly Trp Ser Ala Gly 420 425 430 cgc aag ctg cgc cgt tcc gtc gac ggg ctg ggg cgg gtg ctc ggc gtc 1644 Arg Lys Leu Arg Arg Ser Val Asp Gly Leu Gly Arg Val Leu Gly Val 435 440 445 gag gtg ctc acc gca gcc cgg gcc atc gac atg cgc gag cag gag ccc 1692 Glu Val Leu Thr Ala Ala Arg Ala Ile Asp Met Arg Glu Gln Glu Pro 450 455 460 gcg ccg ggg acc cgc gcc gca ctg gac gcc ctg cgc ggc cgt gtg gag 1740 Ala Pro Gly Thr Arg Ala Ala Leu Asp Ala Leu Arg Gly Arg Val Glu 465 470 475 480 ggg ccc ggc ccc gac cgc tac ctg tcc ccg gag atc gag gag tcg gtg 1788 Gly Pro Gly Pro Asp Arg Tyr Leu Ser Pro Glu Ile Glu Glu Ser Val 485 490 495 cag ctg ctc acc tcc ggg gcg ctg ctg gag gcg gtg gag ggg gtc gtc 1836 Gln Leu Leu Thr Ser Gly Ala Leu Leu Glu Ala Val Glu Gly Val Val 500 505 510 gga aag ctg gca tga gcgggcgaat ctcagatatg agactaaagg ggcctccggg 1891 Gly Lys Leu Ala 515 ccctcgatcc cgactgtgac ctgtcttacc gtcagaagtg tcaccgacac cggccacacc 1951 agcccggtgc tccacacccg acgatgacag ccccgggcca cccggatcgc gggtgccccg 2011 gggccccggc ctgatcggcc cccaccgtag aaggagaccc accccatgac cgagtccccg 2071 cgcaccatcc gtgccccgca cggcaccgag ctcagcgcca agagctggca aaccgaggca 2131 cccctgcgca tgctcatgaa 2151 <210> 194 <211> 516 <212> PRT <213> Corynebacterium humireducens <400> 194 Met Gln Thr Ile Thr Ser His Ile Arg Val Gly Val Gly Ala Leu Ser 1 5 10 15 Ile Asp Glu Val Val Ala Val Ala Arg His Asp Ala His Val Glu Ile 20 25 30 Thr Pro Glu Ala Leu Glu Ala Val Ala Ala Thr Arg Ala Arg Ile Glu 35 40 45 Glu Leu Ala Asp His Pro Thr Pro Val Tyr Gly Val Ser Thr Gly Phe 50 55 60 Gly Ala Leu Ala Arg Arg Tyr Ile Pro Gln Glu Met Arg Leu Gln Leu 65 70 75 80 Gln Arg Ser Leu Val Arg Ser His Ala Ala Gly Ser Gly Pro Glu Val 85 90 95 Glu Arg Glu Val Val Arg Ala Leu Met Leu Leu Arg Leu Ser Thr Leu 100 105 110 Cys Thr Gly Arg Thr Gly Val Arg Pro Val Val Ala Glu Thr Tyr Ala 115 120 125 Ala Val Leu Asn Ala Gly Ile Thr Pro Val Val His Glu Tyr Gly Ser 130 135 140 Leu Gly Cys Ser Gly Asp Leu Ser Pro Leu Ala His Cys Ala Leu Ala 145 150 155 160 Leu Leu Gly Glu Gly Glu Val Thr Thr Ala Asp Gly Arg Arg Leu Pro 165 170 175 Ala Ala Val Ala Leu Ala Glu His Gly Ile Glu Pro Leu Val Leu Arg 180 185 190 Glu Lys Glu Gly Leu Ala Leu Ile Asn Gly Thr Asp Gly Met Leu Gly 195 200 205 Met Leu Cys Leu Ala Ile Ala Asp Leu Glu Cys Leu Ala Arg Ser Ala 210 215 220 Asp Ile Ala Ala Ala Met Thr Val Glu Ala Leu Arg Gly Thr Leu Thr 225 230 235 240 Val Phe Ala Ala Asp Leu Gln Glu Leu Arg Pro His Pro Gly Gln Ala 245 250 255 Ala Ser Ala Ala Asn Ile Arg Ala Val Ala Glu Gly Ser Ala Ile Leu 260 265 270 Gln Ser Ala Thr Glu Glu Phe Ala Leu Thr His Val Gln Asp Ala Tyr 275 280 285 Ser Val Arg Cys Ala Pro Gln Val Ala Gly Gly Phe Arg Asp Thr Leu 290 295 300 Ala His Ala Arg Leu Val Ala Asp Arg Glu Leu Ala Ser Ala Val Asp 305 310 315 320 Asn Pro Val Val Ala Leu Asp Gly Arg Val Thr Ser Asn Gly Asn Phe 325 330 335 His Gly Ala Pro Val Gly Tyr Ala Leu Asp Phe Leu Ala Ile Val Thr 340 345 350 Ala Asp Leu Ala Ser Ile Ser Glu Arg Arg Thr Asp Arg Leu Leu Asp 355 360 365 Val Ala Arg Ser Arg Gly Leu Asn Ala Phe Leu Ala Asp Asp Pro Gly 370 375 380 Val Asp Ser Gly His Met Ile Ala Gln Tyr Thr Gln Ala Gly Ile Val 385 390 395 400 Ser Glu Leu Lys Arg Leu Ala Ala Pro Ala Ser Val Asp Ser Ile Pro 405 410 415 Ser Ser Ala Met Gln Glu Asp His Val Ser Met Gly Trp Ser Ala Gly 420 425 430 Arg Lys Leu Arg Arg Ser Val Asp Gly Leu Gly Arg Val Leu Gly Val 435 440 445 Glu Val Leu Thr Ala Ala Arg Ala Ile Asp Met Arg Glu Gln Glu Pro 450 455 460 Ala Pro Gly Thr Arg Ala Ala Leu Asp Ala Leu Arg Gly Arg Val Glu 465 470 475 480 Gly Pro Gly Pro Asp Arg Tyr Leu Ser Pro Glu Ile Glu Glu Ser Val 485 490 495 Gln Leu Leu Thr Ser Gly Ala Leu Leu Glu Ala Val Glu Gly Val Val 500 505 510 Gly Lys Leu Ala 515 <210> 195 <211> 1509 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1209) <223> gene hutG (Formididoylglutamase) <400> 195 cgagtccgta ggtgcgggtc tcggggatgc gcacgacgta gccctccgac tccatgacgg 60 cgagcaggtg gtacgtcgac gaccggggga ggtcgagttc cgtcatgatg cgtgccgccg 120 agacgggcac gtcgatggat gagagcagcg agaggatgcg caacgtgtgt cgcgcggcgg 180 gcacctgtcc catgtcactc cttacctgtg tgtggtctgt gggtcggcgg tctcgtatgc 240 gggaccacgc tgcgtcgcag ccgggtgtgg cgggaggtcg gtggcgttat cctacggcgc 300 atg acc acc cat gaa tgg aca gga cgc gac gac ggg ccc ggg gcc ggg 348 Met Thr Thr His Glu Trp Thr Gly Arg Asp Asp Gly Pro Gly Ala Gly 1 5 10 15 cac gcc cgt tgg cac agc gtc atc acc acc acg acc gac cgg ccg ggg 396 His Ala Arg Trp His Ser Val Ile Thr Thr Thr Thr Asp Arg Pro Gly 20 25 30 gtc gcc ctc gtc ggg ttc gcc tcc gac gag ggg gtc cgc cgc aac ggt 444 Val Ala Leu Val Gly Phe Ala Ser Asp Glu Gly Val Arg Arg Asn Gly 35 40 45 ggc cgc ccc ggc gcc gcc gcc ggc ccc acg gag atc cgc cgt gcc ctg 492 Gly Arg Pro Gly Ala Ala Ala Gly Pro Thr Glu Ile Arg Arg Ala Leu 50 55 60 gcc tcc ctg gcc gtg cac acc gac gtc ccg ctt ctc gac gcc ggc acc 540 Ala Ser Leu Ala Val His Thr Asp Val Pro Leu Leu Asp Ala Gly Thr 65 70 75 80 gtc acc gtc tcc ggt acc gac ctg gag ggc ggg cac gac gca ctg tcc 588 Val Thr Val Ser Gly Thr Asp Leu Glu Gly Gly His Asp Ala Leu Ser 85 90 95 gcg cag gtg gag gag ctg acc cgc gcg ggg cac acc gtc atc gtg ctg 636 Ala Gln Val Glu Glu Leu Thr Arg Ala Gly His Thr Val Ile Val Leu 100 105 110 ggc ggt ggg cat gag acg gcg ttc ggg tcg cac cgc ggg gtg cgc cgg 684 Gly Gly Gly His Glu Thr Ala Phe Gly Ser His Arg Gly Val Arg Arg 115 120 125 gcg ctg ggg cac agt ccg gcg atc atc aac ctc gac gcc cac ttc gac 732 Ala Leu Gly His Ser Pro Ala Ile Ile Asn Leu Asp Ala His Phe Asp 130 135 140 ctg cgg gtc gcg ccg gcc ccg tcc tcg ggt acc ccg ttc cgg cag atc 780 Leu Arg Val Ala Pro Ala Pro Ser Ser Gly Thr Pro Phe Arg Gln Ile 145 150 155 160 gcg gac ctg gcc ggg gag gag ttc cgt tat gcg gtg ctg ggc atc tcg 828 Ala Asp Leu Ala Gly Glu Glu Phe Arg Tyr Ala Val Leu Gly Ile Ser 165 170 175 gtg ccc aac aac acc cgg gcg ctg ttc gac acc gcc cgg gaa ctg ggg 876 Val Pro Asn Asn Thr Arg Ala Leu Phe Asp Thr Ala Arg Glu Leu Gly 180 185 190 gtc cgg gtg acc acc gac gtc gag ctg gcg gag atg acg tcg gcc ggg 924 Val Arg Val Thr Thr Asp Val Glu Leu Ala Glu Met Thr Ser Ala Gly 195 200 205 tgc gag gcg ctc gtc gga aag ctc ctg gag gac gcc ccg gac gtc cac 972 Cys Glu Ala Leu Val Gly Lys Leu Leu Glu Asp Ala Pro Asp Val His 210 215 220 ctg agc atc gac ctt gac gtc ctg ccc gcg gcg gtc gcc ccc ggc gtg 1020 Leu Ser Ile Asp Leu Asp Val Leu Pro Ala Ala Val Ala Pro Gly Val 225 230 235 240 agc gcg ccg gcg gcg ttg ggc gtc gat ctg gca cgg atc ctg acg ctc 1068 Ser Ala Pro Ala Ala Leu Gly Val Asp Leu Ala Arg Ile Leu Thr Leu 245 250 255 gtc aga gcc gtg gcg cgc agc ggg aag ctg cgt ctg gtc gat gtc gtc 1116 Val Arg Ala Val Ala Arg Ser Gly Lys Leu Arg Leu Val Asp Val Val 260 265 270 gaa ctc aac ccg ctt ctc gac gtc gac ggc cgg acc gcg cgg gtc gcg 1164 Glu Leu Asn Pro Leu Leu Asp Val Asp Gly Arg Thr Ala Arg Val Ala 275 280 285 gcc cgg atc gtg gag gag atc gtg cgt cat cgg ccg tcg gac tga 1209 Ala Arg Ile Val Glu Glu Ile Val Arg His Arg Pro Ser Asp 290 295 300 tcctgcggtt ccgggtggac ctgcaccccc tggatgtagg tccgccacgt gagcggcatc 1269 cccggccggt aggccagatc gatggcgtgg ggggtgtcca gcacgtgcag gtctgccgcg 1329 gccccgacca cgaggctgcc gcgggcgggt cggccctggg ggtcgacgcc gccgccgatg 1389 tcgtgacgcc gcagcgcccg cgccccaccg gcggtggccg cgtggatggc ctcgtcgagg 1449 gtgaggtgct gctgcagcac cgcggtggtg acgcagaagg ccatcgacga ggtgtacgac 1509 <210> 196 <211> 302 <212> PRT <213> Corynebacterium humireducens <400> 196 Met Thr Thr His Glu Trp Thr Gly Arg Asp Asp Gly Pro Gly Ala Gly 1 5 10 15 His Ala Arg Trp His Ser Val Ile Thr Thr Thr Thr Asp Arg Pro Gly 20 25 30 Val Ala Leu Val Gly Phe Ala Ser Asp Glu Gly Val Arg Arg Asn Gly 35 40 45 Gly Arg Pro Gly Ala Ala Ala Gly Pro Thr Glu Ile Arg Arg Ala Leu 50 55 60 Ala Ser Leu Ala Val His Thr Asp Val Pro Leu Leu Asp Ala Gly Thr 65 70 75 80 Val Thr Val Ser Gly Thr Asp Leu Glu Gly Gly His Asp Ala Leu Ser 85 90 95 Ala Gln Val Glu Glu Leu Thr Arg Ala Gly His Thr Val Ile Val Leu 100 105 110 Gly Gly Gly His Glu Thr Ala Phe Gly Ser His Arg Gly Val Arg Arg 115 120 125 Ala Leu Gly His Ser Pro Ala Ile Ile Asn Leu Asp Ala His Phe Asp 130 135 140 Leu Arg Val Ala Pro Ala Pro Ser Ser Gly Thr Pro Phe Arg Gln Ile 145 150 155 160 Ala Asp Leu Ala Gly Glu Glu Phe Arg Tyr Ala Val Leu Gly Ile Ser 165 170 175 Val Pro Asn Asn Thr Arg Ala Leu Phe Asp Thr Ala Arg Glu Leu Gly 180 185 190 Val Arg Val Thr Thr Asp Val Glu Leu Ala Glu Met Thr Ser Ala Gly 195 200 205 Cys Glu Ala Leu Val Gly Lys Leu Leu Glu Asp Ala Pro Asp Val His 210 215 220 Leu Ser Ile Asp Leu Asp Val Leu Pro Ala Ala Val Ala Pro Gly Val 225 230 235 240 Ser Ala Pro Ala Ala Leu Gly Val Asp Leu Ala Arg Ile Leu Thr Leu 245 250 255 Val Arg Ala Val Ala Arg Ser Gly Lys Leu Arg Leu Val Asp Val Val 260 265 270 Glu Leu Asn Pro Leu Leu Asp Val Asp Gly Arg Thr Ala Arg Val Ala 275 280 285 Ala Arg Ile Val Glu Glu Ile Val Arg His Arg Pro Ser Asp 290 295 300 <210> 197 <211> 1767 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1467) <223> gene sucC <400> 197 gctggccggt ccggacttcc ggtacctcga tcacatcaag ccgatctacg accagcccat 60 cggtgaggcg acctggcgcg acatgtccac ctggttcacg tggcagagca ggggagaccg 120 tttcggctac ggccacctga cgtcggaact ctgcagcggt cggctcgggg agctggtgga 180 acgcttcctg gagctgtggg acgcgcccac cacggcgtag cgcctccgtc cggggcgtgt 240 ggccgcaggg tatcgccgat ccggcgtcgt gcgtgcggca acgtctatac actgcagggc 300 atg gac ctc tac gag tac cag gcc ctt gaa ctc ttc gcc gcc cac ggc 348 Met Asp Leu Tyr Glu Tyr Gln Ala Leu Glu Leu Phe Ala Ala His Gly 1 5 10 15 gtt ccg gtt ctg tcc gga acg gtg gct gag act ccg gcg gag gca cgc 396 Val Pro Val Leu Ser Gly Thr Val Ala Glu Thr Pro Ala Glu Ala Arg 20 25 30 gcg gcg gcg gag cgg ctg ggc ggg gag tgc gtc gtc aag gcg cag gtg 444 Ala Ala Ala Glu Arg Leu Gly Gly Glu Cys Val Val Lys Ala Gln Val 35 40 45 aag atc ggc ggc cgc ggc aag gcg ggc ggt gtc cgc gtc gcg gcg gcg 492 Lys Ile Gly Gly Arg Gly Lys Ala Gly Gly Val Arg Val Ala Ala Ala 50 55 60 ccc gag gcg gcc gag cag gcg gcg gag gcg atc ctg ggg atg gac atc 540 Pro Glu Ala Ala Glu Gln Ala Ala Glu Ala Ile Leu Gly Met Asp Ile 65 70 75 80 cgc ggg cac acg gtc gag cgg gtg ctc gtc gtg gag tgc gtg gag atc 588 Arg Gly His Thr Val Glu Arg Val Leu Val Val Glu Cys Val Glu Ile 85 90 95 gag gcg gag tac tac ttc tcg ctg ctc ctc gac cgc gcg cgg cgc tcc 636 Glu Ala Glu Tyr Tyr Phe Ser Leu Leu Leu Asp Arg Ala Arg Arg Ser 100 105 110 tac ctg ggg ttg ttc tcg gtg gag gga ggc gtg gac atc gag aag ctc 684 Tyr Leu Gly Leu Phe Ser Val Glu Gly Gly Val Asp Ile Glu Lys Leu 115 120 125 gcc gcg gag cgg ccg gag gcg ctg gcc cgc gtg gag gtc gac ccc ctc 732 Ala Ala Glu Arg Pro Glu Ala Leu Ala Arg Val Glu Val Asp Pro Leu 130 135 140 gag ggc ttc gac gcc cac gaa ctt gtc gac gcc ctc ccc gcc gac ctc 780 Glu Gly Phe Asp Ala His Glu Leu Val Asp Ala Leu Pro Ala Asp Leu 145 150 155 160 cgc gac aag gtc gtc ccc gtc atc cgg cgg ctc gcc gag gtc ttc cgc 828 Arg Asp Lys Val Val Pro Val Ile Arg Arg Leu Ala Glu Val Phe Arg 165 170 175 gag gag gac gcc acc ctc gtc gag gtg aac ccg ctc gtc ctc acg gtg 876 Glu Glu Asp Ala Thr Leu Val Glu Val Asn Pro Leu Val Leu Thr Val 180 185 190 cag ggg gac atc atc gcc ctc gac gcg aag atc acc ctc gac gac aac 924 Gln Gly Asp Ile Ile Ala Leu Asp Ala Lys Ile Thr Leu Asp Asp Asn 195 200 205 gcc gcc ttc cgt cac gac aac cgc gcg gag ctc gcc gag gcc gcc gac 972 Ala Ala Phe Arg His Asp Asn Arg Ala Glu Leu Ala Glu Ala Ala Asp 210 215 220 cac ctc gac ccg atc gag cga cgc gcg agc gag cag ggg ctc aag tac 1020 His Leu Asp Pro Ile Glu Arg Arg Ala Ser Glu Gln Gly Leu Lys Tyr 225 230 235 240 gtc acg ctc gag ggt tcg gtg ggc atc atc ggc aac ggc gcc ggg ctg 1068 Val Thr Leu Glu Gly Ser Val Gly Ile Ile Gly Asn Gly Ala Gly Leu 245 250 255 gtc atg tcg acc ctc gac gtc gtg tcc ggg gcg ggg gag cgg cac ggc 1116 Val Met Ser Thr Leu Asp Val Val Ser Gly Ala Gly Glu Arg His Gly 260 265 270 ggg cag aag ccg gcg aac ttc ctc gac atc ggc ggt ggc gcc tgc ccg 1164 Gly Gln Lys Pro Ala Asn Phe Leu Asp Ile Gly Gly Gly Ala Cys Pro 275 280 285 gag acg atg acc gcg gcc ctg gag atc gtc ctc tcg gac gag cag gtg 1212 Glu Thr Met Thr Ala Ala Leu Glu Ile Val Leu Ser Asp Glu Gln Val 290 295 300 cgc agc gtg ttc gtc aac gtc ttc gga ggc atc acg tcc tgc gac gcg 1260 Arg Ser Val Phe Val Asn Val Phe Gly Gly Ile Thr Ser Cys Asp Ala 305 310 315 320 gtg gcc acc ggc atc gtg gag gcg ctc act cac ctc ggg gac acg gcc 1308 Val Ala Thr Gly Ile Val Glu Ala Leu Thr His Leu Gly Asp Thr Ala 325 330 335 cgc aag ccg atc gtc gtg cgt ctc gac ggc aac agc gtc gac gag gga 1356 Arg Lys Pro Ile Val Val Arg Leu Asp Gly Asn Ser Val Asp Glu Gly 340 345 350 cgc cgc atc ctc cgc gag tac gat cac ccc ctc gtc acc gtc gtc gac 1404 Arg Arg Ile Leu Arg Glu Tyr Asp His Pro Leu Val Thr Val Val Asp 355 360 365 gac atg gac gag gcc gcg gac cgg gtg gcg gag ctc gcc gcg gtg acc 1452 Asp Met Asp Glu Ala Ala Asp Arg Val Ala Glu Leu Ala Ala Val Thr 370 375 380 ccg agc gtc gtc tag cgaaaggcac cgacatggcg atcttcctca actccgaatc 1507 Pro Ser Val Val 385 ccgcatcatc gtccagggca tcaccgggcg tgaggggcgg gagcacaccg cccggatgct 1567 cgccgccggc gcgaacgtgg tcggcggcac caacccgaag aaggcgggcg agacggtgac 1627 ggtcggcggc cacgagctgc cggtgttcgg caccgtcgcc gacgcacgcg cggccaccga 1687 cgcgaacgtg tccgtggtgt tcgtgcctgc ccccttcgtc cgggaggcgg tctgcgaggc 1747 catcgacgcc gcgatcccgc 1767 <210> 198 <211> 388 <212> PRT <213> Corynebacterium humireducens <400> 198 Met Asp Leu Tyr Glu Tyr Gln Ala Leu Glu Leu Phe Ala Ala His Gly 1 5 10 15 Val Pro Val Leu Ser Gly Thr Val Ala Glu Thr Pro Ala Glu Ala Arg 20 25 30 Ala Ala Ala Glu Arg Leu Gly Gly Glu Cys Val Val Lys Ala Gln Val 35 40 45 Lys Ile Gly Gly Arg Gly Lys Ala Gly Gly Val Arg Val Ala Ala Ala 50 55 60 Pro Glu Ala Ala Glu Gln Ala Ala Glu Ala Ile Leu Gly Met Asp Ile 65 70 75 80 Arg Gly His Thr Val Glu Arg Val Leu Val Val Glu Cys Val Glu Ile 85 90 95 Glu Ala Glu Tyr Tyr Phe Ser Leu Leu Leu Asp Arg Ala Arg Arg Ser 100 105 110 Tyr Leu Gly Leu Phe Ser Val Glu Gly Gly Val Asp Ile Glu Lys Leu 115 120 125 Ala Ala Glu Arg Pro Glu Ala Leu Ala Arg Val Glu Val Asp Pro Leu 130 135 140 Glu Gly Phe Asp Ala His Glu Leu Val Asp Ala Leu Pro Ala Asp Leu 145 150 155 160 Arg Asp Lys Val Val Pro Val Ile Arg Arg Leu Ala Glu Val Phe Arg 165 170 175 Glu Glu Asp Ala Thr Leu Val Glu Val Asn Pro Leu Val Leu Thr Val 180 185 190 Gln Gly Asp Ile Ile Ala Leu Asp Ala Lys Ile Thr Leu Asp Asp Asn 195 200 205 Ala Ala Phe Arg His Asp Asn Arg Ala Glu Leu Ala Glu Ala Ala Asp 210 215 220 His Leu Asp Pro Ile Glu Arg Arg Ala Ser Glu Gln Gly Leu Lys Tyr 225 230 235 240 Val Thr Leu Glu Gly Ser Val Gly Ile Ile Gly Asn Gly Ala Gly Leu 245 250 255 Val Met Ser Thr Leu Asp Val Val Ser Gly Ala Gly Glu Arg His Gly 260 265 270 Gly Gln Lys Pro Ala Asn Phe Leu Asp Ile Gly Gly Gly Ala Cys Pro 275 280 285 Glu Thr Met Thr Ala Ala Leu Glu Ile Val Leu Ser Asp Glu Gln Val 290 295 300 Arg Ser Val Phe Val Asn Val Phe Gly Gly Ile Thr Ser Cys Asp Ala 305 310 315 320 Val Ala Thr Gly Ile Val Glu Ala Leu Thr His Leu Gly Asp Thr Ala 325 330 335 Arg Lys Pro Ile Val Val Arg Leu Asp Gly Asn Ser Val Asp Glu Gly 340 345 350 Arg Arg Ile Leu Arg Glu Tyr Asp His Pro Leu Val Thr Val Val Asp 355 360 365 Asp Met Asp Glu Ala Ala Asp Arg Val Ala Glu Leu Ala Ala Val Thr 370 375 380 Pro Ser Val Val 385 <210> 199 <211> 1455 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1155) <223> gene sucD <400> 199 cctggagatc gtcctctcgg acgagcaggt gcgcagcgtg ttcgtcaacg tcttcggagg 60 catcacgtcc tgcgacgcgg tggccaccgg catcgtggag gcgctcactc acctcgggga 120 cacggcccgc aagccgatcg tcgtgcgtct cgacggcaac agcgtcgacg agggacgccg 180 catcctccgc gagtacgatc accccctcgt caccgtcgtc gacgacatgg acgaggccgc 240 ggaccgggtg gcggagctcg ccgcggtgac cccgagcgtc gtctagcgaa aggcaccgac 300 atg gcg atc ttc ctc aac tcc gaa tcc cgc atc atc gtc cag ggc atc 348 Met Ala Ile Phe Leu Asn Ser Glu Ser Arg Ile Ile Val Gln Gly Ile 1 5 10 15 acc ggg cgt gag ggg cgg gag cac acc gcc cgg atg ctc gcc gcc ggc 396 Thr Gly Arg Glu Gly Arg Glu His Thr Ala Arg Met Leu Ala Ala Gly 20 25 30 gcg aac gtg gtc ggc ggc acc aac ccg aag aag gcg ggc gag acg gtg 444 Ala Asn Val Val Gly Gly Thr Asn Pro Lys Lys Ala Gly Glu Thr Val 35 40 45 acg gtc ggc ggc cac gag ctg ccg gtg ttc ggc acc gtc gcc gac gca 492 Thr Val Gly Gly His Glu Leu Pro Val Phe Gly Thr Val Ala Asp Ala 50 55 60 cgc gcg gcc acc gac gcg aac gtg tcc gtg gtg ttc gtg cct gcc ccc 540 Arg Ala Ala Thr Asp Ala Asn Val Ser Val Val Phe Val Pro Ala Pro 65 70 75 80 ttc gtc cgg gag gcg gtc tgc gag gcc atc gac gcc gcg atc ccg ctc 588 Phe Val Arg Glu Ala Val Cys Glu Ala Ile Asp Ala Ala Ile Pro Leu 85 90 95 atc gtc gtc atc acc gag ggc gtg ccc gtc cgc gac acc gcc gag atg 636 Ile Val Val Ile Thr Glu Gly Val Pro Val Arg Asp Thr Ala Glu Met 100 105 110 ctg gcg cac gcg cgg acg gag gga ggc acg cgg atc atc ggc ccg aac 684 Leu Ala His Ala Arg Thr Glu Gly Gly Thr Arg Ile Ile Gly Pro Asn 115 120 125 tgc ccc ggc atc atc ccc gcg gat atc agc ggc ccc ggc ccc gtg gga 732 Cys Pro Gly Ile Ile Pro Ala Asp Ile Ser Gly Pro Gly Pro Val Gly 130 135 140 ctg atc tcg aag tcg ggc acg ctg acg tac cag atg atg cac gcg ctt 780 Leu Ile Ser Lys Ser Gly Thr Leu Thr Tyr Gln Met Met His Ala Leu 145 150 155 160 tcc gac gtc ggc atc tcc acc gcc ctc ggc atc ggc ggc gac ccc ctc 828 Ser Asp Val Gly Ile Ser Thr Ala Leu Gly Ile Gly Gly Asp Pro Leu 165 170 175 atc ggc acc gcc tac atc gac gcg ctc agc gcc ttc gag gag gac ccc 876 Ile Gly Thr Ala Tyr Ile Asp Ala Leu Ser Ala Phe Glu Glu Asp Pro 180 185 190 gac acc cgg gcc atc gtc atg atc ggc gag atc ggc ggc gac gcc gag 924 Asp Thr Arg Ala Ile Val Met Ile Gly Glu Ile Gly Gly Asp Ala Glu 195 200 205 gaa cgc gcc gcc gac tac atc cgg gag cac gtg acc aaa ccc gtc gtc 972 Glu Arg Ala Ala Asp Tyr Ile Arg Glu His Val Thr Lys Pro Val Val 210 215 220 ggc tac gtc gcc ggg ttc acc gcc ccg gag ggc aag acc atg ggg cac 1020 Gly Tyr Val Ala Gly Phe Thr Ala Pro Glu Gly Lys Thr Met Gly His 225 230 235 240 gca ggt gcc atc gtc acc ggt tcc gtc ggc acg gcg cag gcc aag aag 1068 Ala Gly Ala Ile Val Thr Gly Ser Val Gly Thr Ala Gln Ala Lys Lys 245 250 255 gag gcg ctc gag gcg gcc ggt gtc cgg gtg ggc acc acg ccg acg gag 1116 Glu Ala Leu Glu Ala Ala Gly Val Arg Val Gly Thr Thr Pro Thr Glu 260 265 270 acc gcg gag atc atg cgg ggg att gtc gag ggg atg tag gctgcccggg 1165 Thr Ala Glu Ile Met Arg Gly Ile Val Glu Gly Met 275 280 tcgcgtcggc ggcctatcat cggagcatgc tggagaccgg acgcacccac cccctggcag 1225 acatcattga cacggtcctt gccgacccga cctcgggctc cggctggtgc ctgtacacag 1285 gcccggggat gaccccgggg gagtacctcg tcgacgagta ccccgaggtc ggcgatgacg 1345 ataccgagac ctatccgccg gcggtgaggg aacgaggtct ggactatttc ctcagtggtc 1405 agatgtgcga ggacgtgatc ctcaatctgg accaccaggg atccccactt 1455 <210> 200 <211> 284 <212> PRT <213> Corynebacterium humireducens <400> 200 Met Ala Ile Phe Leu Asn Ser Glu Ser Arg Ile Ile Val Gln Gly Ile 1 5 10 15 Thr Gly Arg Glu Gly Arg Glu His Thr Ala Arg Met Leu Ala Ala Gly 20 25 30 Ala Asn Val Val Gly Gly Thr Asn Pro Lys Lys Ala Gly Glu Thr Val 35 40 45 Thr Val Gly Gly His Glu Leu Pro Val Phe Gly Thr Val Ala Asp Ala 50 55 60 Arg Ala Ala Thr Asp Ala Asn Val Ser Val Val Phe Val Pro Ala Pro 65 70 75 80 Phe Val Arg Glu Ala Val Cys Glu Ala Ile Asp Ala Ala Ile Pro Leu 85 90 95 Ile Val Val Ile Thr Glu Gly Val Pro Val Arg Asp Thr Ala Glu Met 100 105 110 Leu Ala His Ala Arg Thr Glu Gly Gly Thr Arg Ile Ile Gly Pro Asn 115 120 125 Cys Pro Gly Ile Ile Pro Ala Asp Ile Ser Gly Pro Gly Pro Val Gly 130 135 140 Leu Ile Ser Lys Ser Gly Thr Leu Thr Tyr Gln Met Met His Ala Leu 145 150 155 160 Ser Asp Val Gly Ile Ser Thr Ala Leu Gly Ile Gly Gly Asp Pro Leu 165 170 175 Ile Gly Thr Ala Tyr Ile Asp Ala Leu Ser Ala Phe Glu Glu Asp Pro 180 185 190 Asp Thr Arg Ala Ile Val Met Ile Gly Glu Ile Gly Gly Asp Ala Glu 195 200 205 Glu Arg Ala Ala Asp Tyr Ile Arg Glu His Val Thr Lys Pro Val Val 210 215 220 Gly Tyr Val Ala Gly Phe Thr Ala Pro Glu Gly Lys Thr Met Gly His 225 230 235 240 Ala Gly Ala Ile Val Thr Gly Ser Val Gly Thr Ala Gln Ala Lys Lys 245 250 255 Glu Ala Leu Glu Ala Ala Gly Val Arg Val Gly Thr Thr Pro Thr Glu 260 265 270 Thr Ala Glu Ile Met Arg Gly Ile Val Glu Gly Met 275 280 <210> 201 <211> 1569 <212> DNA <213> Corynebacterium humireducens <220> <221> CDS <222> (301)..(1269) <223> gene ddh <400> 201 ggcgccaccc tagcgcctga gccggtttct gacacccgcg gatcctgcgg taccatctgc 60 aggcaggcgg gtacatgtgc ctgccggtgc tggagtggcg caatcggtag cgcaacggtc 120 ttgtaaaccg taggttgcga gttcaagtct cgtctccagc tctgctctcc acgaggtggg 180 gtccgggtga accggccccg cctcgttccc ttttgtggga ctcatttcta ttgactgacc 240 ggtgatgggc cgtagaatgg gcggcagcaa gcaccgtccc ccacctcaag gagtcagcac 300 atg gcc aac atc agg gtc gcg atc gtc ggt tac ggc aac ctc ggc aag 348 Met Ala Asn Ile Arg Val Ala Ile Val Gly Tyr Gly Asn Leu Gly Lys 1 5 10 15 tcc gtc gag aag ctc atc ggc cag cag ccg gac atg gag ctc gcg ggc 396 Ser Val Glu Lys Leu Ile Gly Gln Gln Pro Asp Met Glu Leu Ala Gly 20 25 30 gtg ttc tcg cgt cgc gac gca ctg gac acc ctc gac ccg gcc acc ccg 444 Val Phe Ser Arg Arg Asp Ala Leu Asp Thr Leu Asp Pro Ala Thr Pro 35 40 45 gtc ctg ccc gtc gcg gag ttc ggt gag cac acc gac aag ttc gac gtc 492 Val Leu Pro Val Ala Glu Phe Gly Glu His Thr Asp Lys Phe Asp Val 50 55 60 gcc gtc ctc tgc ctc ggc tcc gcc acc gac atc ccg gag cag gcc ccc 540 Ala Val Leu Cys Leu Gly Ser Ala Thr Asp Ile Pro Glu Gln Ala Pro 65 70 75 80 gag ttc atc cgc cac gcc aac acg gtc gac acc tac gac aac cac cgc 588 Glu Phe Ile Arg His Ala Asn Thr Val Asp Thr Tyr Asp Asn His Arg 85 90 95 gac atc ccg cgc cac cgc gcc gag atg gac agg gtg gcc agg gag aac 636 Asp Ile Pro Arg His Arg Ala Glu Met Asp Arg Val Ala Arg Glu Asn 100 105 110 ggc acc gtc gcc atc gtc tcc acc ggc tgg gac ccg ggc atg ttc tcc 684 Gly Thr Val Ala Ile Val Ser Thr Gly Trp Asp Pro Gly Met Phe Ser 115 120 125 ctc aac cgc acc atc ggc cag gcg ctc ctg ccg ggc ggc cag cag cag 732 Leu Asn Arg Thr Ile Gly Gln Ala Leu Leu Pro Gly Gly Gln Gln Gln 130 135 140 acc ttc tgg ggt ccg ggc ctc tcg cag ggc cac tct gac gcg gtg cgc 780 Thr Phe Trp Gly Pro Gly Leu Ser Gln Gly His Ser Asp Ala Val Arg 145 150 155 160 cgc gtc gcg ggc gtg aag aag ggc gtg cag tac acc atc ccc tcc ccg 828 Arg Val Ala Gly Val Lys Lys Gly Val Gln Tyr Thr Ile Pro Ser Pro 165 170 175 gag gcc ctc gag gcg gcc cgc aag ggt gag tcg ggt ctc agc ggc aag 876 Glu Ala Leu Glu Ala Ala Arg Lys Gly Glu Ser Gly Leu Ser Gly Lys 180 185 190 cag gcc cac ctg cgc cgc tgc ttc atc gtg gcg gac gag gcc gac cac 924 Gln Ala His Leu Arg Arg Cys Phe Ile Val Ala Asp Glu Ala Asp His 195 200 205 gag cgc atc gag aac gag atc cgc acc atg ccc gac tac ttc gtc ggc 972 Glu Arg Ile Glu Asn Glu Ile Arg Thr Met Pro Asp Tyr Phe Val Gly 210 215 220 tac gag gtg gag gtc aac ttc atc gac gag gcc acc ttc gac gcc gag 1020 Tyr Glu Val Glu Val Asn Phe Ile Asp Glu Ala Thr Phe Asp Ala Glu 225 230 235 240 cac acc ggc atg ccg cac ggc ggg cac gtc atc acg gcg ggt gac acc 1068 His Thr Gly Met Pro His Gly Gly His Val Ile Thr Ala Gly Asp Thr 245 250 255 ggc ggc tac gtc cag tcc atc gag tac aac ctc gac ctg gac cgg aac 1116 Gly Gly Tyr Val Gln Ser Ile Glu Tyr Asn Leu Asp Leu Asp Arg Asn 260 265 270 ccg gac ttc acc ggt gcc gtc gcc ctg gca tac gcc cgt gcg gcc cac 1164 Pro Asp Phe Thr Gly Ala Val Ala Leu Ala Tyr Ala Arg Ala Ala His 275 280 285 cgt ctg cgt gag gcg ggc cag gcc ggc gcc tac acc gtc ctc gag gtc 1212 Arg Leu Arg Glu Ala Gly Gln Ala Gly Ala Tyr Thr Val Leu Glu Val 290 295 300 gcc ccg tac ctg atc tcc ccg acg ccg ctc gag gag ctc atc gcc cgc 1260 Ala Pro Tyr Leu Ile Ser Pro Thr Pro Leu Glu Glu Leu Ile Ala Arg 305 310 315 320 gac gtg tag ggtccaccac cgggatcagc aggagggcac cgaccagcag 1309 Asp Val gaccaggccg atgccgagga tcccgaagcg gtcggtgccg cccagcagga cgaacatccc 1369 gaaggcgagc ggcgagagcc acgacacggc gcgtccggtg gtgacgtaca ggccgaagag 1429 ctggccctcc cgcccgtcgg gggcgacgcg ggagaggaac gagcgggccg ccgactgggc 1489 ggggccgacg aacaggcaca ggatgaggcc gaagatccag aacatctgcg ggccctccac 1549 gaagtagagg acgcccgcgg 1569 <210> 202 <211> 322 <212> PRT <213> Corynebacterium humireducens <400> 202 Met Ala Asn Ile Arg Val Ala Ile Val Gly Tyr Gly Asn Leu Gly Lys 1 5 10 15 Ser Val Glu Lys Leu Ile Gly Gln Gln Pro Asp Met Glu Leu Ala Gly 20 25 30 Val Phe Ser Arg Arg Asp Ala Leu Asp Thr Leu Asp Pro Ala Thr Pro 35 40 45 Val Leu Pro Val Ala Glu Phe Gly Glu His Thr Asp Lys Phe Asp Val 50 55 60 Ala Val Leu Cys Leu Gly Ser Ala Thr Asp Ile Pro Glu Gln Ala Pro 65 70 75 80 Glu Phe Ile Arg His Ala Asn Thr Val Asp Thr Tyr Asp Asn His Arg 85 90 95 Asp Ile Pro Arg His Arg Ala Glu Met Asp Arg Val Ala Arg Glu Asn 100 105 110 Gly Thr Val Ala Ile Val Ser Thr Gly Trp Asp Pro Gly Met Phe Ser 115 120 125 Leu Asn Arg Thr Ile Gly Gln Ala Leu Leu Pro Gly Gly Gln Gln Gln 130 135 140 Thr Phe Trp Gly Pro Gly Leu Ser Gln Gly His Ser Asp Ala Val Arg 145 150 155 160 Arg Val Ala Gly Val Lys Lys Gly Val Gln Tyr Thr Ile Pro Ser Pro 165 170 175 Glu Ala Leu Glu Ala Ala Arg Lys Gly Glu Ser Gly Leu Ser Gly Lys 180 185 190 Gln Ala His Leu Arg Arg Cys Phe Ile Val Ala Asp Glu Ala Asp His 195 200 205 Glu Arg Ile Glu Asn Glu Ile Arg Thr Met Pro Asp Tyr Phe Val Gly 210 215 220 Tyr Glu Val Glu Val Asn Phe Ile Asp Glu Ala Thr Phe Asp Ala Glu 225 230 235 240 His Thr Gly Met Pro His Gly Gly His Val Ile Thr Ala Gly Asp Thr 245 250 255 Gly Gly Tyr Val Gln Ser Ile Glu Tyr Asn Leu Asp Leu Asp Arg Asn 260 265 270 Pro Asp Phe Thr Gly Ala Val Ala Leu Ala Tyr Ala Arg Ala Ala His 275 280 285 Arg Leu Arg Glu Ala Gly Gln Ala Gly Ala Tyr Thr Val Leu Glu Val 290 295 300 Ala Pro Tyr Leu Ile Ser Pro Thr Pro Leu Glu Glu Leu Ile Ala Arg 305 310 315 320 Asp Val 201400112A 1

Claims (15)

  1. 효소 GcvP, GcvT 및 GcvH 를 포함하는 글리신 절단 시스템으로서, 상기 시스템이 하기 폴리펩티드 중 적어도 하나를 포함하는 것을 특징으로 하는 시스템:
    a) SEQ ID NO: 40 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 GcvP,
    b) SEQ ID NO: 42 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 GcvT,
    c) SEQ ID NO: 38 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 GcvH.
  2. 제 1 항에 있어서, 상기 시스템이 상기 언급한 폴리펩티드 중 2 개, 바람직하게는 3 개 모두를 포함하는 것을 특징으로 하는 글리신 절단 시스템.
  3. 제 2 항에 있어서, 상기 시스템이 하기 폴리펩티드를 포함하는 것을 특징으로 하는 글리신 절단 시스템:
    a) SEQ ID NO: 40 에 따른 서열에 대해 적어도 95 또는 98% 의 서열 동일성을 갖는 효소 GcvP,
    b) SEQ ID NO: 42 에 따른 서열에 대해 적어도 95 또는 98% 의 서열 동일성을 갖는 효소 GcvT,
    c) SEQ ID NO: 38 에 따른 서열에 대해 적어도 95 또는 98% 의 서열 동일성을 갖는 효소 GcvH.
  4. 제 1 항 내지 제 3 항 중 어느 한 항에 있어서, 상기 시스템이 하기로 이루어진 군으로부터 선택되는 적어도 하나의 추가 폴리펩티드를 포함하는 것을 특징으로 하는 글리신 절단 시스템:
    a) SEQ ID NO: 48 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 LipA,
    b) SEQ ID NO: 50 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 LipB,
    c) SEQ ID NO: 52 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 Lpd,
    d) SEQ ID NO: 94 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 LplA,
    e) SEQ ID NO: 96 에 따른 서열에 대해 적어도 80% 의 서열 동일성을 갖는 효소 GcvL.
  5. 하기로 이루어진 군으로부터 선택되는, 글리신 절단 시스템의 효소를 구성하는 폴리펩티드:
    a) SEQ ID NO: 40 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvP,
    b) SEQ ID NO: 42 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvT,
    c) SEQ ID NO: 38 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvH,
    d) 효소 LipA, 바람직하게는 SEQ ID NO: 48 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LipA,
    e) 효소 LipB, 바람직하게는 SEQ ID NO: 50 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LipB,
    f) 효소 Lpd, 바람직하게는 SEQ ID NO: 52 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 Lpd,
    g) 효소 LplA, 바람직하게는 SEQ ID NO: 94 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 LplA,
    h) 효소 GcvL, 바람직하게는 SEQ ID NO: 96 에 따른 서열에 대해, 적어도 80%, 바람직하게는 적어도 85 또는 90%, 특히 적어도 92, 94, 96 또는 98%, 특히 100% 의 서열 동일성을 갖는 효소 GcvL.
  6. 하기로 이루어진 군으로부터 선택되는, 글리신 절단 시스템의 효소를 코딩하는 폴리뉴클레오티드:
    a) 효소 GcvP 를 코딩하고, SEQ ID NO: 39 에 따른 위치 301 내지 3162 의 서열에 대해, 적어도 70 또는 75%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 39 에 따른 위치 301 내지 3162 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvP);
    b) 효소 GcvT 를 코딩하고, SEQ ID NO: 41 에 따른 위치 301 내지 1404 의 서열에 대해, 적어도 70 또는 75%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 41 에 따른 위치 301 내지 1404 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvT);
    c) 효소 GcvH 를 코딩하고, SEQ ID NO: 37 에 따른 위치 301 내지 699 의 서열에 대해, 적어도 70 또는 75%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 37 에 따른 위치 301 내지 699 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvH);
    d) 효소 LipA 를 코딩하고, SEQ ID NO: 47 에 따른 위치 301 내지 1344 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 47 에 따른 위치 301 내지 1344 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lipA);
    e) 효소 LipB 를 코딩하고, SEQ ID NO: 49 에 따른 위치 301 내지 1089 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 49 에 따른 위치 301 내지 1089 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lipB);
    f) 효소 Lpd 를 코딩하고, SEQ ID NO: 51 에 따른 위치 301 내지 1710 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 51 에 따른 위치 301 내지 1710 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lpd);
    g) 효소 LplA 를 코딩하고, SEQ ID NO: 93 에 따른 위치 301 내지 1089 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 93 에 따른 위치 301 내지 1089 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (lplA);
    h) 효소 GcvL 를 코딩하고, SEQ ID NO: 95 에 따른 위치 301 내지 1710 의 서열에 대해, 적어도 70%, 바람직하게는 적어도 80 또는 85%, 특히 적어도 90 또는 95%, 특히 적어도 98 또는 100% 의 서열 동일성을 갖고/갖거나 SEQ ID NO: 95 에 따른 위치 301 내지 1710 의 서열에 상보적인 서열의 폴리뉴클레오티드와 엄격한 조건 하에서 혼성화하는 폴리뉴클레오티드 (gcvL).
  7. 제 6 항에 따른 적어도 1 개의 폴리뉴클레오티드, 바람직하게는 적어도 2 또는 3 개의 폴리뉴클레오티드를 포함하는 것을 특징으로 하는 벡터.
  8. 미생물이 제 1 항 내지 제 4 항 중 어느 한 항에 따른 적어도 하나의 글리신 절단 시스템 및/또는 상기 글리신 절단 시스템을 코딩하는 폴리뉴클레오티드 및/또는 제 5 항에 따른 적어도 하나의 폴리펩티드 및/또는 상기 폴리펩티드를 코딩하는 폴리뉴클레오티드 또는 상기 폴리펩티드들을 코딩하는 폴리뉴클레오티드들 및/또는 제 6 항에 따른 적어도 하나의 폴리뉴클레오티드 및/또는 제 7 항에 따른 벡터를 포함하는 것을 특징으로하는 재조합 미생물.
  9. 제 8 항에 있어서, 글리신 절단 시스템 및/또는 글리신 절단 시스템을 코딩하는 폴리뉴클레오티드가 과발현 형태로 존재하는 것을 특징으로 하는 재조합 미생물.
  10. 제 8 항 또는 제 9 항에 있어서, 상기 미생물이 하기 특징 중 적어도 1 개, 바람직하게는 적어도 2 또는 3 개, 특히 바람직하게는 적어도 4 또는 5 개를 갖는 L-메티오닌-과잉생산 균주인 것을 특징으로 하는 재조합 미생물:
    a) HTH tetR 형의 DNA 결합 도메인 (McbR), 바람직하게는 SEQ ID NO: 2 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 DNA 결합 도메인을 코딩하는 감쇠된 폴리뉴클레오티드 (mcbR),
    b) 호모세린 키나아제 (ThrB, EC 2.7.1.39), 바람직하게는 SEQ ID NO: 4 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 키나아제를 코딩하는 감쇠된 폴리뉴클레오티드 (thrB 유전자),
    c) 글루코오스-6-포스페이트 이소메라아제 (Pgi, EC 5.3.1.9), 바람직하게는 SEQ ID NO: 6 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 글루코오스-6-포스페이트 이소메라아제를 코딩하는 감쇠된 폴리뉴클레오티드 (pgi),
    d) 포스포에놀피루베이트 카르복시키나아제 (Pck, EC 4.1.1.32), 바람직하게는 SEQ ID NO: 8 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포에놀피루베이트 카르복시키나아제를 코딩하는 감쇠된 폴리뉴클레오티드 (pck),
    e) D-메티오닌-결합 지질단백질 (MetQ), 바람직하게는 SEQ ID NO: 10 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 D-메티오닌-결합 지질단백질을 코딩하는 감쇠된 폴리뉴클레오티드 (metQ),
    f) 메티오닌 운반체 (MetP), 바람직하게는 SEQ ID NO: 12 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 메티오닌 운반체를 코딩하는 감쇠된 폴리뉴클레오티드 (metP),
    g) ATP-의존성 메티오닌 운반체 (MetN), 바람직하게는 SEQ ID NO: 14 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 ATP-의존성 메티오닌 운반체를 코딩하는 감쇠된 폴리뉴클레오티드 (metN),
    h) S-아데노실메티오닌 신타아제 (MetK), 바람직하게는 SEQ ID NO: 16 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 S-아데노실메티오닌 신타아제를 코딩하는 감쇠된 폴리뉴클레오티드 (metK),
    i) 메티오닌 도입 시스템 퍼미아제 (MetI), 바람직하게는 SEQ ID NO: 18 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 메티오닌 도입 시스템 퍼미아제를 코딩하는 감쇠된 폴리뉴클레오티드 (metI),
    j) 4-히드록시-테트라히드로디피콜리네이트 신타아제 (DapA), 바람직하게는 SEQ ID NO: 20 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 4-히드록시-테트라히드로디피콜리네이트 신타아제를 코딩하는 감쇠된 폴리뉴클레오티드 (dapA),
    k) 시스테인 신타아제 (CBS), 바람직하게는 SEQ ID NO: 22 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스테인 신타아제를 코딩하는 과발현 폴리뉴클레오티드 (CBS),
    l) cg3031 동족체, 바람직하게는 SEQ ID NO: 24 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 cg3031 동족체를 코딩하는 감쇠된 폴리뉴클레오티드,
    m) 시스타티오닌 베타-리아제 (AecD), 바람직하게는 SEQ ID NO: 26 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 베타-리아제를 코딩하는 과발현 폴리뉴클레오티드 (aecD),
    n) 아스파르테이트 세미알데히드 디히드로게나아제 (Asd), 바람직하게는 SEQ ID NO: 28 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 세미알데히드 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (asd),
    o) 5-메틸테트라히드로폴레이트 호모시스테인 메틸트랜스페라아제 (MetH, EC 2.1.1.13) 를 코딩하는 과발현 폴리뉴클레오티드 (metH),
    p) 분지쇄 아미노산에 대한 운반체의 보다 작은 서브유닛 (BrnE), 바람직하게는 SEQ ID NO: 30 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (brnE),
    q) 분지쇄 아미노산에 대한 운반체의 보다 큰 서브유닛 (BrnF), 바람직하게는 SEQ ID NO: 32 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (brnF),
    r) 세린 아세틸트랜스페라아제 (CysE), 바람직하게는 SEQ ID NO: 34 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 세린 아세틸트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (cysE),
    s) 시스테인 신타아제 (CysK), 바람직하게는 SEQ ID NO: 36 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스테인 신타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysK),
    t) 세린 히드록시메틸트랜스페라아제 (GlyA), 바람직하게는 SEQ ID NO: 44 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 세린 히드록시메틸트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (glyA),
    u) 임의적 피드백-내성 호모세린 디히드로게나아제 (Hom), 바람직하게는 SEQ ID NO: 46 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (hom),
    v) 임의로는 피드백-내성 아스파르테이트 키나아제 (LysC), 바람직하게는 SEQ ID NO: 54 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아스파르테이트 키나아제를 코딩하는 과발현 폴리뉴클레오티드 (lysC),
    w) 시스타티오닌 감마-신타아제 (MetB), 바람직하게는 SEQ ID NO: 56 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 감마-신타아제를 코딩하는 과발현 폴리뉴클레오티드 (metB),
    x) 5,10-메틸렌테트라히드로폴레이트 리덕타아제 (MetF), 바람직하게는 SEQ ID NO: 58 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 5,10-메틸렌테트라히드로폴레이트 리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (metF),
    y) 호모세린 O-아세틸트랜스페라아제 (MetX), 바람직하게는 SEQ ID NO: 60 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 호모세린 O-아세틸트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (metX),
    z) O-아세틸호모세린 리아제 (MetY), 바람직하게는 SEQ ID NO: 62 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 O-아세틸호모세린 리아제를 코딩하는 과발현 폴리뉴클레오티드 (metY),
    aa) 피루베이트 카르복실라아제 (Pyc), 바람직하게는 SEQ ID NO: 64 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 피루베이트 카르복실라아제를 코딩하는 과발현 폴리뉴클레오티드 (pyc),
    bb) 임의적 피드백-내성 D-3-포스포글리세레이트 디히드로게나아제 (SerA), 바람직하게는 SEQ ID NO: 66 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 D-3-포스포글리세레이트 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (serA),
    cc) 포스포세린 포스파타아제 (SerB), 바람직하게는 SEQ ID NO: 68 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포세린 포스파타아제를 코딩하는 과발현 폴리뉴클레오티드 (serB),
    dd) 포스포세린 아미노트랜스페라아제 (SerC), 바람직하게는 SEQ ID NO: 70 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 포스포세린 아미노트랜스페라아제를 코딩하는 과발현 폴리뉴클레오티드 (serC),
    ee) 알라닌 디히드로게나아제 (Ald), 바람직하게는 SEQ ID NO: 72 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 알라닌 디히드로게나아제를 코딩하는 과발현 폴리뉴클레오티드 (ald),
    ff) 술페이트 아데닐릴트랜스페라아제의 서브유닛 (CysD), 바람직하게는 SEQ ID NO: 74 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (cysD),
    gg) 아데노신 포스포술페이트 리덕타아제 (CysH), 바람직하게는 SEQ ID NO: 76 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 아데노신 포스포술페이트 리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysH),
    hh) 술파이트 리덕타아제 (CysI), 바람직하게는 SEQ ID NO: 78 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술파이트 리덕타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysI),
    ii) (CysJ), 바람직하게는 SEQ ID NO: 80 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 것을 코딩하는 과발현 폴리뉴클레오티드 (cysJ),
    jj) 술페이트 아데닐릴트랜스페라아제의 서브유닛 (CysN), 바람직하게는 SEQ ID NO: 82 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 서브유닛을 코딩하는 과발현 폴리뉴클레오티드 (cysN),
    kk) 시스타티오닌 베타-신타아제 (CysY), 바람직하게는 SEQ ID NO: 84 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 시스타티오닌 베타-신타아제를 코딩하는 과발현 폴리뉴클레오티드 (cysY),
    ll) 추정 상의 (putative) 술페이트 운반체 (CysZ), 바람직하게는 SEQ ID NO: 86 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 술페이트 운반체를 코딩하는 과발현 폴리뉴클레오티드 (cysZ),
    mm) 5-메틸테트라히드로프테로일트리글루타메이트-호모시스테인 메틸트랜스페라아제 (MetE), 바람직하게는 SEQ ID NO: 88 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 단백질을 코딩하는 과발현 폴리뉴클레오티드 (metE),
    nn) 펩티딜-tRNA 히드롤라아제 1 (PtH1), 바람직하게는 SEQ ID NO: 90 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 펩티딜-tRNA 히드롤라아제 1 을 코딩하는 과발현 폴리뉴클레오티드 (ptH1),
    oo) 펩티딜-tRNA 히드롤라아제 2 (PtH2), 바람직하게는 SEQ ID NO: 92 에 따른 서열에 대해, 적어도 90, 95 또는 98%, 바람직하게는 100% 의 서열 동일성을 갖는 펩티딜-tRNA 히드롤라아제 2 를 코딩하는 과발현 폴리뉴클레오티드 (ptH2).
  11. 제 8 항 내지 제 10 항 중 어느 한 항에 있어서, 상기 미생물이 코리네형 박테리아, 특히 코리네박테리움, 바람직하게는 C. 글루타미쿰 또는 C. 휴미레듀센스인 것을 특징으로 하는 재조합 미생물.
  12. 제 1 항 내지 제 4 항 중 어느 한 항에 따른 글리신 절단 시스템 및/또는 제 5 항에 따른 적어도 하나의 폴리펩티드 및/또는 제 6 항에 따른 적어도 하나의 폴리뉴클레오티드 및/또는 제 7 항에 따른 벡터 및/또는 제 8 항 내지 제 11 항 중 어느 한 항에 따른 재조합 미생물이 방법에서 사용되는 것을 특징으로 하는 L-아미노산의 과잉생산 방법.
  13. 제 12 항에 있어서, 과잉생산된 L-아미노산이 L-알라닌, L-발린, 글루타메이트 패밀리의 L-아미노산, 특히 L-글루타메이트, L-글루타민, L-프롤린 및 L-아르기닌, 및 아스파르테이트 패밀리의 L-아미노산, 특히 L-아스파르테이트, L-아스파라긴, L-메티오닌, L-리신, L-이소류신 및 L-트레오닌으로부터 선택되는 것을 특징으로 하는 방법.
  14. 제 13 항에 있어서, 과잉생산된 L-아미노산이 L-메티오닌인 것을 특징으로 하는 방법.
  15. 제 12 항 내지 제 14 항 중 어느 한 항에 있어서, 단지 적은 양의 글리신이 부산물로서 발생하는 방법.
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PCT/EP2015/058280 WO2015165740A2 (de) 2014-04-30 2015-04-16 Verfahren zur produktion von l-aminosäuren in corynebakterien unter verwendung eines glycin spaltenden systems

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