KR20200056434A - 조직-선호적 프로모터 및 이용 방법 - Google Patents

조직-선호적 프로모터 및 이용 방법 Download PDF

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KR20200056434A
KR20200056434A KR1020207011474A KR20207011474A KR20200056434A KR 20200056434 A KR20200056434 A KR 20200056434A KR 1020207011474 A KR1020207011474 A KR 1020207011474A KR 20207011474 A KR20207011474 A KR 20207011474A KR 20200056434 A KR20200056434 A KR 20200056434A
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알렉산드르 다 실바 콘세이상
앨리슨 엠. 핀치
윌리암 제임스 고든-캄
테오도르 엠. 클레인
키스 에스. 로위
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Abstract

식물에서 이종 뉴클레오티드 서열의 발현을 조절하기 위한 조성물 및 방법이 제공된다. 조성물은 형태형성 유전자에 작동 가능하게 연결되는 조직-선호적 프로모터를 포함한다. 형태형성 유전자에 작동 가능하게 연결되는 본원에 개시된 프로모터 서열을 이용해 식물에서 이종 뉴클레오티드 서열을 발현시키는 방법이 또한 제공된다. 형태형성 유전자에 작동 가능하게 연결되는 프로모터를 포함하고 이종의 관심 뉴클레오티드 서열을 추가로 포함하는 DNA 구축물이 또한 제공된다.

Description

조직-선호적 프로모터 및 이용 방법
본 발명은 식물 분자 생물학 분야, 보다 구체적으로는 식물의 유전자 발현 조절에 관한 것이다.
관련 출원의 상호 참조
본 출원은 2017년 9월 25일 출원된 미국 가출원 62/562,663호의 우선권을 주장하며, 그 전문은 본원에 참조로 포함된다.
전자적으로 제출된 서열 목록에 대한 참조
서열 목록의 공식 사본은 2018년 8월 29일 작성되어 본 명세서와 동시에 제출된 995,556 바이트 크기의 파일명 20180829_7453WOPCT_ST25의 ASCII 형식 서열 목록으로서 EFS-웹을 통해 전자적으로 제출되었다. 이 ASCII 형식의 문서에 포함된 서열 목록은 본 명세서의 일부이며, 그 전체가 본원에 참조로 포함된다.
식물 숙주에서의 이종 DNA 서열의 발현은 식물 숙주 내에서 기능적인 프로모터를 포함해 작동 가능하게 연결되는 프로모터의 존재에 의존적이다. 프로모터 서열의 선택은 유기체 내에서 이종 DNA 서열이 발현되는 시기 및 위치를 결정할 것이다. 특정 조직 또는 기관에서의 발현이 요구되는 경우, 조직-선호적 프로모터가 사용될 수 있다. 자극에 대한 반응으로 유전자 발현이 요구되는 경우, 유도성 프로모터가 조절 요소로서 선택된다. 반면, 식물의 세포 전체에 걸쳐 연속적인 발현이 요구되는 경우, 항시성 프로모터가 사용된다. 유전자이식 식물에서 이종 뉴클레오티드 서열의 다양한 수준의 발현을 유도하기 위해 코어 프로모터 서열로부터의 상류 및/또는 하류의 추가적인 조절 서열이 형질전환 벡터의 발현 구축물에 포함될 수 있다.
흔히 식물의 특정 조직 또는 기관에서 DNA 서열을 발현시키는 것이 바람직하다. 예를 들어, 세포 증식을 촉진하는 형태형성 유전자에 작동 가능하게 연결되는 조직-선호적 프로모터의 사용은 형질전환 과정 중 유전자이식 이벤트의 효율적 재생에 유용하다. 이러한 조직-선호적 프로모터는 또한, 원하는 식물 조직에서 형질 유전자 및/또는 병원체 저항성 단백질을 발현시켜 식물 수확량 및 병원체에 대한 저항성을 향상시키는 데 유용하다. 대안적으로, 원하는 표현형을 달성하기 위해 식물 조직 내에서 천연 DNA 서열의 발현을 억제하는 것이 바람직할 수 있다. 이 경우, 이러한 억제는 안티센스 서열의 발현이 천연 DNA 서열의 mRNA의 번역을 방해하는 RNA 전사체를 생성하도록 안티센스 뉴클레오티드 서열에 작동 가능하게 연결되는 조직-선호적 프로모터를 포함하도록 식물을 형질전환시켜 달성될 수 있다.
또한, 예를 들어 세포 분열 또는 신장과 같은 특정 성장 또는 발육 단계에 있는 식물 조직에서 DNA 서열을 발현시키는 것이 바람직할 수 있다. 이러한 DNA 서열은 식물 성장 과정을 촉진하거나 억제하는 데 사용되어 식물의 성장 속도 또는 구조에 영향을 미칠 수 있다.
따라서, 조직-선호적 프로모터, 특히 아그로박테리움-매개 형질전환 직후 시험관내 성장 및 형태형성을 자극하도록 유전자를 폭발적으로 발현시킨 후, 이소성 과발현이 비정상적인 성장 및 발육을 유발하는 식물 조직에서 감소되고 사실상 "제거"되는, 형태형성 유전자를 포함한 성장 자극 유전자의 제어된 발현을 위한 조절 요소로서 작용할 수 있는 프로모터의 단리 및 특성화가 필요하다.
식물에서 유전자 발현을 조절하기 위한 조성물 및 방법이 제공된다. 보다 구체적으로, 본 발명의 프로모터는 식물 조직의 표피 L1(외층)에서의 조직-선호적 발현을 부여한다. 본 발명의 특정 양태는, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 단편 또는 변이체; 및 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 갖는 조직-선호적 프로모터를 포함하는 형태형성 유전자 카세트를 포함하는 핵산 분자로서, 조직-선호적 프로모터가 형태형성 유전자에 작동 가능하게 연결되는, 핵산 분자를 포함한다. 형태형성 유전자 카세트를 포함하는 발현 카세트가 또한 포함된다. 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하는 벡터가 또한 포함된다.
또한, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 단편 또는 변이체; 및 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 갖는 조직-선호적 프로모터를 포함하는 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하고, 조직-선호적 프로모터가 형태형성 유전자에 작동 가능하게 연결되는, 식물 세포 및 식물이 제공된다. 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하는 식물 세포 및 식물은 외떡잎식물, 쌍떡잎식물, 및 겉씨식물을 포함한다. 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하는 식물 세포 및 식물은 외떡잎식물, 쌍떡잎식물, 및 겉씨식물(옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 및 배추속을 포함하나, 이에 한정되지 않음)을 포함한다. 본 발명은 또한, 형태형성 유전자 카세트의 형태형성 유전자가 WUS/WOX 호메오박스 폴리펩티드를 암호화하는(WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화됨), 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하는 식물 세포 또는 식물을 제공한다. 형태형성 유전자 카세트의 형태형성 유전자가 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하는 식물 세포 또는 식물이 또한 제공된다.
본 발명은 또한, 발현 카세트가 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종 폴리뉴클레오티드를 포함하는 형질 유전자 카세트를 추가로 포함하는, 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하는 식물 세포 및 식물을 제공한다. 본 발명은 또한, 발현 카세트가 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종 폴리뉴클레오티드를 포함하는 형질 유전자 카세트, 및 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소가 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 형태형성 유전자 카세트를 포함하는 발현 카세트를 포함하는 식물 세포 및 식물을 제공한다. UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 및 발육 조절 프로모터가 또한 제공된다. 발현 카세트의 형태형성 유전자 카세트 및 부위-특이적 재조합효소 카세트가 식물 세포 및 식물에서 일시적으로 발현되고, 발현 카세트의 형질 유전자 카세트가 식물 세포 및 식물의 게놈에 안정적으로 통합되는, 식물 세포 및 식물이 또한 제공된다. 발현 카세트의 형태형성 유전자 카세트 및 부위-특이적 재조합효소 카세트가 식물 세포 및 식물로부터 절제되고, 발현 카세트의 형질 유전자 카세트가 식물 세포 및 식물의 게놈에 안정적으로 통합되는, 식물 세포 및 식물이 또한 제공된다. 발현 카세트의 형질 유전자 카세트를 포함하는 식물 종자가 또한 제공된다.
본 발명은 또한, 식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열이 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드를 가지며, 전사를 개시할 수 있는 뉴클레오티드 서열이 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트를 제공한다.
식물 또는 식물 세포에서 전사를 개시할 수 있는 기능적 단편 또는 변이체를 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 기능적 단편 또는 변이체가 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열로부터 유도되고, 전사를 개시할 수 있는 기능적 단편 또는 변이체가 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트가 또한 제공된다.
본 발명은 또한, 식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열이 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열과 적어도 70%의 동일성을 가지며, 전사를 개시할 수 있는 뉴클레오티드 서열이 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트를 제공한다.
식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열이 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열과 적어도 95%의 동일성을 가지며, 전사를 개시할 수 있는 뉴클레오티드 서열이 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트가 또한 제공된다.
식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열이 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되고, 전사를 개시할 수 있는 뉴클레오티드 서열이 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트가 또한 제공된다.
유전자이식 식물의 제조 방법으로서, (a) 조직-선호적 프로모터 카세트, 및 (b) 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종의 관심 폴리뉴클레오티드를 포함하는 형질 유전자 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계(조직-선호적 프로모터 카세트는, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 또는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 포함하고, 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결됨); 재조합 발현 카세트를 갖는 유전자이식 식물 세포를 선별하는 단계; 및 유전자이식 식물 세포로부터 유전자이식 식물을 재생시키는 단계를 포함하는 방법이 또한 제공된다.
본 발명의 유전자이식 식물 제조 방법에 유용한 외떡잎식물 세포, 쌍떡잎식물 세포, 및 겉씨식물 세포가 또한 제공된다. 본 발명의 방법에 유용한 식물 세포는 외떡잎식물, 쌍떡잎식물, 및 겉씨식물(옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 및 배추속을 포함하나, 이에 한정되지 않음)을 포함한다.
본 발명은 또한, 유전자이식 식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 형태형성 유전자가 WUS/WOX 호메오박스 폴리펩티드를 암호화하는(WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화됨), 방법을 제공한다. 유전자이식 식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 형태형성 유전자가 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 방법이 또한 제공된다.
유전자이식 식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 재조합 발현 카세트가 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소가 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법이 또한 제공된다. 유전자이식 식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트 및 부위-특이적 재조합효소 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터가 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법이 또한 제공된다.
유전자이식 식물의 제조 방법으로서, 재조합 발현 카세트로부터 조직-선호적 프로모터 카세트 및 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는 방법이 또한 제공된다. 본원에 개시된 방법에 의해 제조되는 유전자이식 식물이 또한 제공된다. 본원에 개시된 방법에 의해 제조된 유전자이식 식물로부터 생성되고, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 식물의 제조 방법으로서, 조직-선호적 프로모터 카세트가 제1 T-DNA를 포함하고 형질 유전자 카세트가 제2 T-DNA를 포함하는, 방법이 또한 제공된다. 유전자이식 식물의 제조 방법으로서, 제1 T-DNA 및 제2 T-DNA가 식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법이 또한 제공된다. 유전자이식 식물의 제조 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 식물이 또한 제공된다. 이렇게 제조된 유전자이식 식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 식물의 제조 방법으로서, 제1 T-DNA가 제1 박테리아 균주에 존재하고 제2 T-DNA가 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주가 식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법이 또한 제공된다. 유전자이식 식물의 제조 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 식물이 또한 제공된다. 이렇게 제조된 유전자이식 식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
체세포배 성숙 효율을 향상시키는 방법으로서, (a) 조직-선호적 프로모터 카세트, 및 (b) 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종의 관심 폴리뉴클레오티드를 포함하는 형질 유전자 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계(조직-선호적 프로모터 카세트는, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 또는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 포함하고, 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결됨); 재조합 발현 카세트를 갖는 유전자이식 식물 세포를 선별하는 단계; 및 유전자이식 식물 세포로부터 유전자이식 식물을 재생시키는 단계를 포함하고, 재조합 발현 카세트로 인해 재조합 발현 카세트를 포함하지 않는 유전자이식 식물 세포에 비해 체세포배 성숙 효율이 향상되는, 방법.
본 발명의 체세포배 성숙 효율을 향상시키는 방법에 유용한 외떡잎식물 세포, 쌍떡잎식물 세포, 및 겉씨식물 세포가 또한 제공된다. 본 발명의 방법에 유용한 식물 세포는 외떡잎식물, 쌍떡잎식물, 및 겉씨식물(옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 및 배추속을 포함하나, 이에 한정되지 않음)을 포함한다.
본 발명은 또한, 체세포배 성숙 효율을 향상시키는 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 형태형성 유전자가 WUS/WOX 호메오박스 폴리펩티드를 암호화하는(WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화됨), 방법을 제공한다. 체세포배 성숙 효율을 향상시키는 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 형태형성 유전자가 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 방법이 또한 제공된다.
체세포배 성숙 효율을 향상시키는 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 재조합 발현 카세트가 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소가 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법이 또한 제공된다. 체세포배 성숙 효율을 향상시키는 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트 및 부위-특이적 재조합효소 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터가 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법이 또한 제공된다.
체세포배 성숙 효율을 향상시키는 방법으로서, 재조합 발현 카세트로부터 조직-선호적 프로모터 카세트 및 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는 방법이 또한 제공된다. 본원에 개시된 방법에 의해 제조되는 유전자이식 식물이 또한 제공된다. 본원에 개시된 방법에 의해 제조된 유전자이식 식물로부터 생성되고, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
체세포배 성숙 효율을 향상시키는 방법으로서, 조직-선호적 프로모터 카세트가 제1 T-DNA를 포함하고 형질 유전자 카세트가 제2 T-DNA를 포함하는, 방법이 또한 제공된다. 체세포배 성숙 효율을 향상시키는 방법으로서, 제1 T-DNA 및 제2 T-DNA가 식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법이 또한 제공된다. 체세포배 성숙 효율을 향상시키는 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 식물이 또한 제공된다. 이렇게 제조된 유전자이식 식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
체세포배 성숙 효율을 향상시키는 방법으로서, 제1 T-DNA가 제1 박테리아 균주에 존재하고 제2 T-DNA가 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주가 식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법이 또한 제공된다. 체세포배 성숙 효율을 향상시키는 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 식물이 또한 제공된다. 이렇게 제조된 유전자이식 식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, (a) 조직-선호적 프로모터 카세트, 및 (b) 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종의 관심 폴리뉴클레오티드를 포함하는 형질 유전자 카세트를 포함하는 재조합 발현 카세트로 쌍떡잎식물 세포 또는 겉씨식물 세포를 형질전환시키는 단계(조직-선호적 프로모터 카세트는, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 또는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 포함하고, 식물세포에서 전사를 개시하는 뉴클레오티드 서열은 WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열에 작동 가능하게 연결됨); 각각의 형질전환된 식물 세포에서 재조합 발현 카세트를 발현시켜 체세포배 또는 싹을 형성하는 단계; 및 체세포배를 발아시키거나 싹을 배양하여, 이종의 관심 폴리뉴클레오티드를 포함하는 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물을 형성하는 단계를 포함하는 방법이 또한 제공된다. 개시된 방법은 또한, 쌍떡잎식물 세포 또는 겉씨식물 세포의 형질전환 개시 후 약 21일 내지 약 28일 내에 체세포배 또는 싹의 형성을 제공한다.
본 발명의 유전자이식 식물 제조 방법에 유용한 겉씨식물 세포가 또한 제공된다. 본 발명의 방법에 유용한 겉씨식물 세포(소나무 및 미송을 포함하나, 이에 한정되지 않음)가 또한 제공된다. 본 발명의 유전자이식 식물 제조 방법에 유용한 쌍떡잎식물 세포가 또한 제공된다. 본 발명의 방법에 유용한 쌍떡잎식물 세포(알팔파, 대두, 목화, 해바라기, 아마, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 멜론, 또는 배추속을 포함하나, 이에 한정되지 않음)가 또한 제공된다.
본 발명은 또한, 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 쌍떡잎식물 세포 또는 겉씨식물 세포를 형질전환시키는 단계를 포함하고, WUS/WOX 호메오박스 폴리펩티드가 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, WUS/WOX 호메오박스 폴리펩티드가 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 방법을 제공한다. 유전자이식 식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계를 포함하고, WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열이 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 방법이 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트를 포함하는 재조합 발현 카세트로 쌍떡잎식물 세포 또는 겉씨식물 세포를 형질전환시키는 단계를 포함하고, 재조합 발현 카세트가 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소가 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 본원에 개시된 조직-선호적 프로모터 카세트 및 부위-특이적 재조합효소 카세트를 포함하는 재조합 발현 카세트로 쌍떡잎식물 세포 또는 겉씨식물 세포를 형질전환시키는 단계를 포함하고, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터가 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법이 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 재조합 발현 카세트로부터 조직-선호적 프로모터 카세트 및 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는 방법이 또한 제공된다. 본원에 개시된 방법에 의해 제조되는 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물이 또한 제공된다. 본원에 개시된 방법에 의해 제조된 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물로부터 생성되고, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 조직-선호적 프로모터 카세트가 제1 T-DNA를 포함하고 형질 유전자 카세트가 제2 T-DNA를 포함하는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제1 T-DNA 및 제2 T-DNA가 식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 식물이 또한 제공된다. 이렇게 제조된 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제1 T-DNA가 제1 박테리아 균주에 존재하고 제2 T-DNA가 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주가 쌍떡잎식물 세포 또는 겉씨식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 식물이 또한 제공된다. 이렇게 제조된 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, (a) 이종의 관심 유전자를 포함하는 형질 유전자 카세트 및 WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열을 포함하는 형태형성 유전자 카세트를 포함하는 재조합 발현 카세트로 쌍떡잎식물 외식편 또는 겉씨식물 외식편의 세포를 형질전환시키는 단계; (b) 각각의 형질전환된 세포에서 (a)의 재조합 발현 카세트를 발현시켜 체세포배 또는 싹을 형성하는 단계; 및 (c) 체세포배를 발아시키거나 싹을 배양하여 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물을 형성하는 단계를 포함하는 방법이 또한 제공된다. 개시된 방법은 또한, 쌍떡잎식물 세포 또는 겉씨식물 세포의 형질전환 개시 후 약 21일 내지 약 28일 내에 체세포배 또는 싹의 형성을 제공한다.
본 발명의 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물 제조 방법에 유용한 쌍떡잎식물 세포 및 겉씨식물 세포가 또한 제공된다. 본 발명의 방법에 유용한 식물 세포는 쌍떡잎식물 및 겉씨식물(알팔파, 대두, 목화, 해바라기, 아마, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 멜론, 또는 배추속을 포함하나, 이에 한정되지 않음)을 포함한다.
본 발명은 또한, 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, WUS/WOX 호메오박스 폴리펩티드가 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, WUS/WOX 호메오박스 폴리펩티드가 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 방법을 제공한다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, WUS/WOX 호메오박스 폴리펩티드가 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는 뉴클레오티드에 의해 암호화되는, 방법이 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, WUS/WOX 호메오박스 폴리펩티드가 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터로 이루어진 군으로부터 선택되는 프로모터에 작동 가능하게 연결되는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, LEA-D34, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체, 또는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편으로 이루어진 군으로부터 선택되는, 방법이 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 이종의 관심 폴리뉴클레오티드가 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는, 방법이 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 재조합 발현 카세트는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소가 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법이 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 재조합 발현 카세트로부터 형태형성 유전자 카세트 및 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는 방법이 또한 제공된다. 본원에 개시된 방법에 의해 제조되는 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물이 또한 제공된다. 본원에 개시된 방법에 의해 제조된 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물로부터 생성되고, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 형태형성 유전자 카세트가 제1 T-DNA를 포함하고 형질 유전자 카세트가 제2 T-DNA를 포함하는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제1 T-DNA 및 제2 T-DNA가 식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 쌍떡잎식물 및 유전자이식 겉씨식물이 또한 제공된다. 이렇게 제조된 유전자이식 쌍떡잎식물 및 유전자이식 겉씨식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제1 T-DNA가 제1 박테리아 균주에 존재하고 제2 T-DNA가 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주가 식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법이 또한 제공된다. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법이 또한 제공된다. 이렇게 제조된 유전자이식 식물이 또한 제공된다. 이렇게 제조된 유전자이식 쌍떡잎식물 및 유전자이식 겉씨식물의 종자로서, 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자가 또한 제공된다.
유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서, 발아시키는 단계가 체세포배 또는 싹을 성숙 배지 또는 발아 배지로 옮겨 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물을 형성하는 단계를 포함하는, 방법이 또한 제공된다.
도 1은 WUS 발현(HBS2 (PHP80734; 서열 번호 113), HBS3(PHP81343; 서열 번호 116), LTP3(PHP80730; 서열 번호 112), MATE1(PHP80736; 서열 번호 114) 및 NED1(PHP81060; 서열 번호 115))을 유도하는 프로모터 GM-HBSTART2(HBS2; 서열 번호 108), GM-HBSTART3(HBS3; 서열 번호 1), GM-LTP3(LTP3; 서열 번호 124), GM-MATE1(MATE1; 서열 번호 109), GM-NED1(NED1; 서열 번호 110), 및 TAG-RFP 대조군(WUS 유전자 없음)(PHP80728; 서열 번호 111)의 체세포배 성숙 효율 평균을 나타낸다.
도 2의 A, 도 2의 B, 및 도 2의 C는 백색광에서 찍은 사진이다. 도 2의 D, 도 2의 E, 및 도의 F는 유전자이식 이벤트를 나타내기 위해 형광에서 찍은 사진이다. 도 2의 A, 도 2의 B, 도 2의 C, 도 2의 D, 도 2의 E, 및 도 2의 F는 동시(아그로박테리움 감염 후 5~6주차)에 찍은 사진이다. TAG-RFP 대조군 처리(대조군 TAG-RFP; PHP80728; 서열 번호 111)인 도 2의 A 및 도 2의 D에 나타낸 체세포배는 작고 발육부전을 보인다. LTP3 PRO::WUS(PHP80730; 서열 번호 112) 처리인 도 2의 B 및 도 2의 E에 나타낸 체세포배는 TAG-RFP 대조군 처리에서보다 약 2배 더 크다. HBS3 PRO::WUS(PHP81343; 서열 번호 116) 처리인 도 2의 C 및 도 2의 F에 나타낸 체세포배는 TAG-RFP 대조군 처리에서보다 약 5배 더 크다.
도 3a 및 도 3b는 GM-HBS3 PRO::WUS 이벤트의 정상적인 발육 및 종자 세트를 보여준다.
전부는 아니지만 일부 가능한 양태가 도시된 첨부 도면을 참조하여 이하 본 발명을 보다 상세하게 설명한다. 실제, 본 발명은 여러 다양한 형태로 구현될 수 있으며, 본 발명이 본원에 제시된 양태로 제한되는 것으로 해석되어서는 안 되고, 오히려 이들 양태는 본 발명이 해당되는 법적 요건을 충족하도록 제공된다.
하기 설명 및 관련 도면에 제시된 교시의 이점을 가지며 개시된 조성물 및 방법과 관련된 당업자는 본원에 개시된 많은 변형예 및 다른 양태를 인식할 것이다. 따라서, 본 발명이 개시된 특정 양태로 제한되는 것은 아니며 변형예 및 다른 양태도 첨부된 청구범위의 범주 내에 포함되는 것으로 이해되어야 한다. 본원에서 특정 용어가 사용되지만, 이들은 일반적이고 설명적인 의미로만 사용되고 제한의 목적으로 사용되지 않는다.
또한, 본원에 사용된 용어는 단지 특정 양태를 설명하기 위한 것이고 제한적 의미가 아닌 것으로 이해되어야 한다. 본 명세서 및 청구범위에 사용된 "포함하는"이란 용어는 "~로 이루어진"의 양태를 포함할 수 있다. 달리 정의되지 않는 한, 본원에 사용된 모든 기술 용어 및 과학 용어는 개시된 조성물 및 방법이 속하는 기술 분야의 당업자가 일반적으로 이해하는 것과 동일한 의미를 갖는다. 본 명세서 및 하기 청구범위에서, 본원에 정의될 다수의 용어가 참조될 것이다.
재생 가능한 식물 구조는 완전히 기능적인 가임 식물을 형성할 수 있는 다세포 구조(예컨대, 이에 한정되는 것은 아니지만, 싹 분열조직, 싹, 체세포배, 배발생 캘러스, 체세포 분열조직, 및/또는 기관형성 캘러스)로서 정의된다.
체세포배는 구형 및 전이기 배아의 형성, 배축과 배반의 형성, 및 지질과 전분의 축적을 포함한 접합배의 발육과 유사한 발육기를 통해 진행되는 다세포 구조로서 정의된다. 접합배에서 유래된 단일 체세포배는 발아하여, 원래 단일 세포에서 유래될 수 있는 단일 비키메라 식물을 생성한다.
배발생 캘러스는 성숙한 가임 식물로 재생될 수 있는 증식하는 1차 및 2차 체세포배와 마주하는 미분화 또는 부분 미분화 세포의 이쇄성 또는 비이쇄성 혼합물로서 정의된다.
체세포 분열조직은, 원시세포로 플랭킹되고 혈관 시원세포와 마주하는 미분화 정단 돔을 갖는 것을 특징으로 하는, 종자-유래 배아의 일부인 정단 분열조직과 유사한 다세포 구조로서 정의되며, 정점 돔은 지상 생장 식물을 발생시킨다. 이러한 체세포 분열조직은 단일의 또는 융합된 클러스터의 분열조직을 형성할 수 있다.
기관형성 캘러스는 분화된 성장 식물 구조(정단 분열조직, 뿌리 분열조직, 잎, 및 뿌리를 포함하나, 이에 한정되지 않음)의 치밀한 혼합물로서 정의된다.
발아는 계속 성장하여 식물을 생성하는 묘목을 형성하기 위한 재생 가능한 구조의 성장이다.
유전자이식 식물은 이식유전자를 함유하는 성숙한 가임 식물로서 정의된다.
본 발명은 형태형성 유전자에 작동 가능하게 연결되는 조직-선호적 프로모터를 포함하는 핵산 분자가 얻어지는 조성물과 방법, 및 이들의 이용 방법에 관한 것이다. 본 발명의 핵산 분자 조성물은 형태형성 유전자에 작동 가능하게 연결되는 GM-HBSTART3(서열 번호 1), GM-HBSTART3(TRUNCATED)(서열 번호 2), AT-ML1(서열 번호 3), GM-ML1-유사(서열 번호 4), GM-ML1-유사(TRUNCATED)(서열 번호 5), ZM-HBSTART3(서열 번호 6), OS-HBSTART3(서열 번호 7), AT-PDF1 P2(서열 번호 8), GM-PDF1(서열 번호 9), GM-PDF1(TRUNCATED)(서열 번호 10), SB-PDF1(서열 번호 11), OS-PDF1(서열 번호 12), OS-PDF1(TRUNCATED)(서열 번호 13), PT-PDF1(서열 번호 14), PT-PDF1(TRUNCATED)(서열 번호 15), SI-PDF1(서열 번호 16), SI-PDF1(TRUNCATED)(서열 번호 17), AT-PDF2(서열 번호 18), GM-PDF2(서열 번호 19), GM-PDF2(TRUNCATED)(서열 번호 20), ZM-GL1(서열 번호 21), AT-PDF2a(서열 번호 22), AT-PDF2a(TRUNCATED)(서열 번호 23), GM-PDF2a(서열 번호 24), GM-PDF2a(TRUNCATED)(서열 번호 25), OS-PDF2(서열 번호 26), OS-PDF2(TRUNCATED)(서열 번호 27), PT-PDF2(서열 번호 28), PT-PDF2(TRUNCATED)(서열 번호 29), VV-PDF2(서열 번호 30), VV-PDF2(TRUNCATED)(서열 번호 31), ZM-PDF2(서열 번호 32), SI-PDF2(서열 번호 33), SI-PDF2(TRUNCATED)(서열 번호 34), VV-PDF2a(서열 번호 35), PT-PDF2a(서열 번호 36), PT-PDF2a(TRUNCATED)(서열 번호 37), MT-PDF2(서열 번호 38), MT-PDF2(TRUNCATED)(서열 번호 39), AT-HDG2(서열 번호 40), GM-HDG2(서열 번호 41), GM-HDG2(TRUNCATED)(서열 번호 42), SB-HDG2(서열 번호 43), SB-HDG2(TRUNCATED)(서열 번호 44), AT-CER6(서열 번호 45), AT-CER60(서열 번호 46), AT-CER60(TRUNCATED)(서열 번호 47), GM-CER6(서열 번호 48), GM-CER6(TRUNCATED)(서열 번호 49), PT-CER6(서열 번호 50), PT-CER6(TRUNCATED)(서열 번호 51), VV-CER6(서열 번호 52), VV-CER6(TRUNCATED)(서열 번호 53), SB-CER6(서열 번호 54), ZM-CER6(서열 번호 55), SI-CER6(서열 번호 56), SI-CER6(TRUNCATED)(서열 번호 57), OS-CER6(서열 번호 58), OS-CER6(TRUNCATED)(서열 번호 59), GM-HBSTART2(서열 번호 108), GM-MATE1(서열 번호 109), GM-NED1(서열 번호 110), GM-LTP3(서열 번호 124), AT-ML1(TRUNCATED)(서열 번호 125), AT-CER6(TRUNCATED1)(서열 번호 126), AT-PDF1(TRUNCATED)(서열 번호 149), AT-PDF2(TRUNCATED)(서열 번호 150), AT-HDG2(TRUNCATED)(서열 번호 151), AT-ANL2(서열 번호 152), 및 AT-CER6(TRUNCATED2)(서열 번호 189)으로 알려진 조직-선호적 프로모터에 대한 뉴클레오티드 서열을 포함한다. 본 발명은 형태형성 유전자에 작동 가능하게 연결되는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189에 제시된 뉴클레오티드 서열 중 적어도 하나와 이의 단편 및 변이체를 포함하는 핵산 분자를 제공한다. 조성물은 형태형성 유전자에 작동 가능하게 연결되는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189에 제시된 뉴클레오티드 서열 중 적어도 하나의 뉴클레오티드 서열을 포함하는 핵산 분자를 포함하는 발현 카세트, DNA 구축물, 및 벡터를 추가로 포함한다.
본원에 사용된 용어 "본원에 개시된 조직-선호적 프로모터"는, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 단편 또는 변이체; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드; 및 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100 bp 이상의 단편으로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 의미한다.
본원에 사용된 용어 "형태형성 유전자"는 이소성 발현시 식물을 생성할 수 있는 체세포-유래 구조의 형성을 자극하는 유전자를 의미한다. 보다 정확하게, 형태형성 유전자의 이소성 발현은 식물을 생성할 수 있는 체세포배 또는 싹 분열조직과 같은 기관형성 구조의 드노보 형성을 자극한다. 이러한 자극된 드노보 형성은 형태형성 유전자가 발현되는 세포, 또는 인접 세포에서 일어난다. 형태형성 유전자는 다른 유전자, 또는 식물 조직의 호르몬 수준에 영향을 미치는 유전자의 발현을 조절하는 전사 인자일 수 있으며, 이들 유전자는 모두 형태형성적 변화를 자극할 수 있다. 형태형성 유전자는 식물의 게놈에 안정적으로 통합되거나, 일시적으로 발현될 수 있다. 본 발명의 조직-선호적 프로모터는 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 형태형성 유전자, 예컨대 WUS/WOX 유전자(WUS1, WUS2, WUS3, WOX2A, WOX4, WOX5, 또는 WOX9)를 발현시키는 데 사용된다(미국 특허 7,348,468 및 7,256,322, 미국 특허 출원 공보 20170121722 및 20070271628; Laux et al. (1996) Development 122:87-96; Mayer et al. (1998) Cell 95:805-815; van der Graaff et al., 2009, Genome Biology 10:248; Dolzblasz et al., 2016, Mol. Plant 19:1028-39 참조). WUS/WOX의 조절은 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합 등, 식물 및/또는 식물 조직 표현형을 조절할 것으로 예상된다. 애기장대 WUS의 발현은 체세포배로 분화될 수 있는 생장 조직에서 줄기 세포를 유도할 수 있다(Zuo, et al. (2002) Plant J 30:349-359). 또한, 이와 관련하여 MYB118 유전자(미국 특허 7,148,402 참조), MYB115 유전자(Wang et al. (2008) Cell Research 224-235 참조), BABYBOOM 유전자(BBM; Boutilier et al. (2002) Plant Cell 14:1737-1749 참조), 또는 CLAVATA 유전자(예를 들어, 미국 특허 7,179,963 참조)가 관심의 대상이다.
본 발명에 유용한 형태형성 유전자는 본원에 개시된 것뿐만 아니라 당업계에 알려진 WUS/WOX 유전자를 포함하나, 이에 한정되지 않는다. 형태형성 유전자는 AT-WUS(서열 번호 60), LJ-W(서열 번호 62), GM-W(서열 번호 64), CS-WUS(서열 번호 66), CR-WUS(서열 번호 68), AA-WUS(서열 번호 70), RS-WUS(서열 번호 72), BN-WUS(서열 번호 74), BO-WU(서열 번호 76), HA-WUS(서열 번호 78), PT-WUS(서열 번호 80), VV-WUS(서열 번호 82), AT-WUS(대두 최적화)(서열 번호 84), LJ-WUS(대두 최적화)(서열 번호 86), MT-WUS(대두 최적화)(서열 번호 88), PY-WUS(대두 최적화)(서열 번호 90), PV-WUS(대두 최적화)(서열 번호 92), ZM-WUS1(서열 번호 94), ZM-WUS2(서열 번호 96), ZM-WUS3(서열 번호 98), ZM-WOX2A(서열 번호 100), ZM-WOX4(서열 번호 102), ZM-WOX5A(서열 번호 104), ZM-WOX9(서열 번호 106), GG- WUS(서열 번호 127), MD-WUS(서열 번호 129), ME-WUS(서열 번호 131), KF-WUS(서열 번호 133), GH-WUS(서열 번호 135), ZOSMA-WUS(서열 번호 137), AMBTR-WUS(서열 번호 139), AC-WUS(서열 번호 141), AH-WUS(서열 번호 143), CUCSA-WUS(서열 번호 145), 및 PINTA-WUS(서열 번호 147) 등, 본원에 개시된 WUS/WOX 유전자를 포함하나, 이에 한정되지 않는다.
본원에 개시된 WUS/WOX 유전자는 AT-WUS(서열 번호 61), LJ-W(서열 번호 63), GM-W(서열 번호 65), CS-WUS(서열 번호 67), CR-WUS(서열 번호 69), AA-WUS(서열 번호 71), RS-WUS(서열 번호 73), BN-WUS(서열 번호 75), BO-WU(서열 번호 77), HA-WUS(서열 번호 79), PT-WUS(서열 번호 81), VV-WUS(서열 번호 83), AT-WUS(서열 번호 85), LJ-WUS(서열 번호 87), MT-WUS(서열 번호 89), PY-WUS(서열 번호 91), PV-WUS(서열 번호 93), ZM-WUS1(서열 번호 95), ZM-WUS2(서열 번호 97), ZM-WUS3(서열 번호 99), ZM-WOX2A(서열 번호 101), ZM-WOX4(서열 번호 103), ZM-WOX5A(서열 번호 105), ZM-WOX9(서열 번호 107), GG- WUS(서열 번호 128), MD-WUS(서열 번호 130), ME-WUS(서열 번호 132), KF-WUS(서열 번호 134), GH-WUS(서열 번호 136), ZOSMA-WUS(서열 번호 138), AMBTR-WUS(서열 번호 130), AC-WUS(서열 번호 142), AH-WUS(서열 번호 144), CUCSA-WUS(서열 번호 146), 및 PINTA-WUS(서열 번호 148)를 포함하는 WUS/WOX 호메오박스 폴리펩티드를 암호화한다.
본원에 개시된 WUS/WOX 유전자는 WUS/WOX 호메오박스 폴리펩티드를 암호화하는 것들을 포함하며, WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, WUS/WOX 호메오박스 폴리펩티드는 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화된다.
본 발명에 유용한 다른 형태형성 유전자는 LEC1(Lotan et al., 1998, Cell 93:1195-1205), LEC2(Stone et al., 2008, PNAS 105:3151-3156; Belide et al., 2013, Plant Cell Tiss. Organ Cult 113:543-553), KN1/STM(Sinha et al., 1993. Genes Dev 7:787-795), 아그로박테리움의 IPT 유전자(Ebinuma and Komamine, 2001, In vitro Cell. Dev Biol - Plant 37:103-113), 모노프테로스-델타(Ckurshumova et al., 2014, New Phytol. 204:556-566), 아그로박테리움 AV-6b 유전자(Wabiko and Minemura 1996, Plant Physiol. 112:939-951), 아그로박테리움 IAA-h 및 IAA-m 유전자의 조합(Endo et al., 2002, Plant Cell Rep., 20:923-928), 애기장대 SERK 유전자(Hecht et al., 2001, Plant Physiol. 127:803-816), 애기장대 AGL15 유전자(Harding et al., 2003, Plant Physiol. 133:653-663)를 포함하나, 이에 한정되지 않는다.
본원에 사용된 용어 "전사 인자"는 프로모터의 DNA 서열에 결합하여 발현을 상향 조절하거나 하향 조절함으로써 특정 유전자의 전사 속도를 제어하는 단백질을 의미한다. 형태형성 유전자이기도 한 전사 인자의 예는 AP2/EREBP 계열의 멤버(BBM(ODP2), plethora 및 aintegumenta 하위계열, CAAT-박스 결합 단백질, 예컨대 LEC1 및 HAP3 포함), 그리고 MYB, bHLH, NAC, MADS, bZIP 및 WRKY 계열의 멤버를 포함한다.
일 양태에서, 재조합 발현 카세트 또는 구축물은 WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열을 포함한다. 다양한 양태에서, WUS/WOX 호메오박스를 암호화하는 뉴클레오티드 서열의 발현은 형질전환의 개시 후 약 21일 내지 약 28일 동안 일어난다.
일 양태에서, WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열은 부위-특이적 재조합효소에 의한 절제의 표적이 될 수 있다. 따라서, WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열의 발현은 형질전환 후 원하는 시기에 절제에 의해 제어될 수 있다. 부위-특이적 재조합효소를 사용하여 WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열의 발현을 제어하는 경우, 발현 구축물은 절제될 폴리뉴클레오티드 서열을 플랭킹하는 적절한 부위-특이적 절제 부위(예를 들어, Cre 재조합효소가 사용될 경우 Cre lox 부위)를 포함하는 것으로 이해된다. WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열을 포함하는 발현 구축물의 동일 위치에 부위-특이적 재조합효소가 존재할 필요는 없다. 그러나, 일 양태에서, 발현 구축물은 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 추가로 포함한다.
WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열의 발현을 제어하는 데 사용되는 부위-특이적 재조합효소는 다양한 적합한 부위-특이적 재조합효소로부터 선택될 수 있다. 예를 들어, 다양한 양태에서, 부위-특이적 재조합효소는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2(Nern et al., (2011) PNAS Vol. 108, No. 34 pp 14198-14203), B3(Nern et al., (2011) PNAS Vol. 108, No. 34 pp 14198-14203), Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153이다. 부위-특이적 재조합효소는 불안정화된 융합 폴리펩티드일 수 있다. 불안정화된 융합 폴리펩티드는 TETR(G17A)~CRE 또는 ESR(G17A)~CRE일 수 있다.
일 양태에서, 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열은 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결된다. 적합한 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 및 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO (ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2(Kalla et al., 1994. Plant J. 6:849-860 및 US5525716), HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34를 포함한다.
일 양태에서, 부위-특이적 재조합효소에 작동 가능하게 연결되는 화학 유도성 프로모터는 XVE이다. 화학 유도성 프로모터는 테트라사이클린 리프레서(TETR), 에타메트설푸론 리프레서(ESR), 또는 클로르설푸론 리프레서(CR)에 의해 억제될 수 있고, 테트라사이클린-관련 또는 설포닐우레아 리간드의 첨가시 탈억제가 일어난다. 리프레서는 TETR일 수 있고, 테트라사이클린-관련 리간드는 독시사이클린 또는 무수테트라사이클린이다(Gatz, C., Frohberg, C. and Wendenburg, R. (1992) Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants, Plant J. 2, 397-404).
대안적으로, 리프레서는 ESR일 수 있고, 설포닐우레아 리간드는 에타메트설푸론, 클로르설푸론, 메트설푸론-메틸, 설포메투론 메틸, 클로리무론 에틸, 니코설푸론, 프리미설푸론, 트리베누론, 설포설푸론, 트리플록시설푸론, 포람설푸론, 요오도설푸론, 프로설푸론, 티펜설푸론, 림설푸론, 메소설푸론, 또는 할로설푸론이다(본원에 전문이 참조로 포함되는 US20110287936).
일 양태에서, 발현 구축물이 부위-특이적 재조합효소 절제 부위를 포함하는 경우, WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열은 옥신 유도성 프로모터, 발육 조절 프로모터, 조직-특이적 프로모터, 또는 항시성 프로모터에 작동 가능하게 연결될 수 있다. 이와 관련하여 유용한 예시적 옥신 유도성 프로모터, 발육 조절 프로모터, 조직-특이적 프로모터, 및 항시성 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1(본원에 전문이 참조로 포함되는 US 6,838,593), DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811(Takahashi, T, et al., (1992) Plant Physiol. 99 (2): 383-390), AT-HSP811L(Takahashi, T, et al., (1992) Plant Physiol. 99 (2): 383-390), GM-HSP173B(
Figure pct00001
(1984) EMBO J. 3(11): 2491-2497), 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, LEA-D34, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체, 또는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편을 포함한다.
WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열의 발현에 적절한 지속 시간은 본원에 개시된 조직-선호적 프로모터(서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체, 또는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편을 포함하나, 이에 한정되지 않음)를 사용함으로써 달성될 수 있다. 유전자이식 식물을 제조하기 위해 형태형성 유전자 카세트 및 형질 유전자 카세트를 사용하는 경우, 형질 유전자만을 함유하는 유전자이식 식물을 제공하기 위해 공동 형질전환 식물의 형질 유전자좌로부터 형태형성 유전자좌를 분리하는 능력을 갖는 것이 바람직하다. 이는 아그로박테리움 투메파시엔스 2 T-DNA 바이너리 시스템을 이용하여 달성될 수 있으며, 이 일반적인 주제에는 두 가지 변형예가 있다(Miller et al., 2002 참조). 예를 들어, 첫 번째 변형예는, 형태형성 유전자 및 제초제 선택(즉, HRA)을 위한 발현 카세트가 제1 T-DNA 내에 들어있고 형질 유전자 카세트가 제2 T-DNA 내에 들어있는 2 T-DNA 벡터이고, 두 T-DNA는 모두 하나의 바이너리 벡터에 존재한다. 2 T-DNA 플라스미드를 함유하는 아그로박테리움에 의해 식물 세포가 형질전환되는 경우, 형질전환된 세포의 많은 퍼센트가 서로 다른 게놈 위치(예를 들어, 서로 다른 염색체)에 통합된 두 T-DNA를 모두 포함한다. 두 번째 방법에서는, 예를 들어 각각 두 T-DNA 중 하나(형태형성 유전자 T-DNA 또는 형질 유전자 T-DNA)를 함유하는 2개의 아그로박테리움 균주가 일정 비율로 혼합되고, 그 혼합물이 형질전환에 사용된다. 이러한 혼합 아그로박테리움 방법을 이용한 형질전환 후, 재생된 유전자이식 이벤트가 보통 별개의 게놈 위치에서 두 T-DNA를 모두 함유하는 경우가 높은 빈도로 관찰된다. 두 공동 형질전환 방법 모두에 대해, 생성된 유전자이식 이벤트의 많은 비율에서, 2개의 T-DNA 좌위가 독립적으로 분리되고, T-DNA 좌위가 서로 분리된 자손 T1 식물이 쉽게 확인될 수 있으며, 결과적으로 형태형성 유전자/제초제 유전자가 없는 형질 유전자 함유 자손 종자가 재생되는 것으로 관찰된다. 문헌[Miller et al. Transgenic Res 11(4):381-96] 참조.
본원에 제공된 방법은 관심 뉴클레오티드 서열이 통합된 재생 가능한 식물 세포를 생성하기 위해 박테리아-매개 및/또는 유전자총-매개 유전자 전달의 사용을 필요로 한다. 본 발명의 방법에 유용한 박테리아 균주는 병원성이 제거된(disarmed) Agrobacteria, Ochrobactrum 박테리아, 또는 Rhizobiaceae 박테리아를 포함하나, 이에 한정되지 않는다. 일 양태에서, 상이한 박테리아 균주는 (i) 병원성이 제거된 Agrobacteria와 Ochrobactrum 박테리아, (ii) 병원성이 제거된 Agrobacteria와 Rhizobiaceae 박테리아, 및 (iii) Rhizobiaceae 박테리아와 Ochrobactrum 박테리아로부터 선택된다.
본 발명의 방법에 유용한, 병원성이 제거된 Agrobacteria는 AGL-1, EHA105, GV3101, LBA4404, 및 LBA4404 THY-를 포함하나, 이에 한정되지 않는다.
본 발명의 방법에 유용한 Ochrobactrum 박테리아 균주는 Ochrobactrum haywardense H1 NRRL Deposit B-67078, Ochrobactrum cytisi, Ochrobactrum daejeonense, Ochrobactrum oryzae, Ochrobactrum tritici LBNL124-A-10, HTG3-C-07, Ochrobactrum pecoris, Ochrobactrum ciceri, Ochrobactrum gallinifaecis, Ochrobactrum grignonense, Ochrobactrum guangzhouense, Ochrobactrum haematophilum, Ochrobactrum intermedium, Ochrobactrum lupini, Ochrobactrum pituitosum, Ochrobactrum pseudintermedium, Ochrobactrum pseudogrignonense, Ochrobactrum rhizosphaerae, Ochrobactrum thiophenivorans, 및 Ochrobactrum tritici를 포함하나, 이에 한정되지 않는다.
본 발명의 방법에 유용한 Rhizobiaceae 박테리아 균주는 Rhizobium lusitanum, Rhizobium rhizogenes, Agrobacterium rubi, Rhizobium multihospitium, Rhizobium tropici, Rhizobium miluonense, Rhizobium leguminosarum, Rhizobium leguminosarum bv. trifolii, Rhizobium leguminosarum bv. phaseoli, Rhizobium leguminosarum. bv. viciae, Rhizobium leguminosarum Madison, Rhizobium leguminosarum USDA2370, Rhizobium leguminosarum USDA2408, Rhizobium leguminosarum USDA2668, Rhizobium leguminosarum 2370G, Rhizobium leguminosarum 2370LBA, Rhizobium leguminosarum 2048G, Rhizobium leguminosarum 2048LBA, Rhizobium leguminosarum bv. phaseoli 2668G, Rhizobium leguminosarum bv. phaseoli 2668LBA, Rhizobium leguminosarum RL542C, Rhizobium etli USDA 9032, Rhizobium etli bv. phaseoli, Rhizobium endophyticum, Rhizobium tibeticum, Rhizobium etli, Rhizobium pisi, Rhizobium phaseoli, Rhizobium fabae, Rhizobium hainanense, Arthrobacter viscosus, Rhizobium alamii, Rhizobium mesosinicum, Rhizobium sullae, Rhizobium indigoferae, Rhizobium gallicum, Rhizobium yanglingense, Rhizobium mongolense, Rhizobium oryzae, Rhizobium loessense, Rhizobium tubonense, Rhizobium cellulosilyticum, Rhizobium soli, Neorhizobium galegae, Neorhizobium vignae, Neorhizobium huautlense, Neorhizobium alkalisoli, Aureimonas altamirensis, Aureimonas frigidaquae, Aureimonas ureilytica, Aurantimonas coralicida, Fulvimarina pelagi, Martelella mediterranea, Allorhizobium undicola, Allorhizobium vitis, Allorhizobium borbor, Beijerinckia fluminensis, Agrobacterium larrymoorei, Agrobacterium radiobacter, Rhizobium selenitireducens corrig, Rhizobium rosettiformans, Rhizobium daejeonense, Rhizobium aggregatum, Pararhizobium capsulatum, Pararhizobium giardinii, Ensifer mexicanus, Ensifer terangae, Ensifer saheli, Ensifer kostiensis, Ensifer kummerowiae, Ensifer fredii, Sinorhizobium americanum, Ensifer arboris, Ensifer garamanticus, Ensifer meliloti, Ensifer numidicus, Ensifer adhaerens, Sinorhizobium sp., Sinorhizobium meliloti SD630, Sinorhizobium meliloti USDA1002, Sinorhizobium fredii USDA205, Sinorhizobium fredii SF542G, Sinorhizobium fredii SF4404, 및 Sinorhizobium fredii SM542C를 포함하나, 이에 한정되지 않는다. 본원에 전문이 참조로 포함되는 US 9,365,859 참조.
일 양태에서, 제1 박테리아 균주와 제2 박테리아 균주는 50:50의 비율로 존재한다. 일 양태에서, 제1 박테리아 균주와 제2 박테리아 균주는 25:75의 비율로 존재한다. 일 양태에서, 제1 박테리아 균주와 제2 박테리아 균주는 10:90의 비율로 존재한다. 일 양태에서, 제1 박테리아 균주와 제2 박테리아 균주는 5:95의 비율로 존재한다. 일 양태에서, 제1 박테리아 균주와 제2 박테리아 균주는 1:99의 비율로 존재한다. 일 양태에서, 제1 박테리아 균주와 제2 박테리아 균주는 상이한 박테리아 균주이다.
본 발명의 프로모터는 식물에서 전사의 개시를 가능하게 하는 뉴클레오티드 서열을 포함한다. 특정 양태에서, 프로모터는 조직-선호적 방식으로 전사의 개시를 가능하게 한다. 본 발명의 구축물은 형태형성 유전자에 작동 가능하게 연결되는 본원에 개시된 조직-선호적 프로모터를 포함한다.
본원에 개시된 조직-선호적 프로모터는 또한, 관심 식물에서 이종의 폴리뉴클레오티드 또는 관심 폴리뉴클레오티드를 후속 발현시키기 위한 발현 카세트 또는 벡터의 구축물에 사용되거나, 다른 프로모터의 단리를 위한 프로브로서 사용될 수 있다. 본 발명은 형태형성 유전자에 작동 가능하게 연결되는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나에 제시된 프로모터 뉴클레오티드 서열을 포함(선택적으로, 이종의 폴리뉴클레오티드 또는 관심 폴리뉴클레오티드를 추가로 포함)하는 단리된 DNA 구축물을 제공한다.
본 발명의 양태는, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 단편 또는 변이체; 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드; 및 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100 bp 이상의 단편으로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 갖는 프로모터를 포함(선택적으로, 이종의 폴리뉴클레오티드 또는 관심 폴리뉴클레오티드를 추가로 포함)하는 핵산 분자로서, 프로모터가 형태형성 유전자에 작동 가능하게 연결되는, 핵산 분자를 포함한다. 또한, 핵산을 함유하는 프로모터를 포함하는 발현 카세트, 발현 카세트를 포함하는 벡터, 및 발현 카세트를 포함하는 식물 세포가 구현된다. 다른 양태는 발현 카세트가 일시적으로 발현되거나 식물 세포 또는 식물의 게놈에 안정적으로 통합되는 외떡잎 또는 쌍떡잎 식물 세포 또는 식물, 및 상기 발현 카세트를 포함하는 외떡잎 또는 쌍떡잎 식물을 포함하며, 외떡잎 또는 쌍떡잎 식물 세포 또는 식물은 옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 및 배추속을 포함한다.
또한, 상기 발현 카세트가 식물의 게놈에 안정적으로 통합된 식물, 발현 카세트를 포함하는 식물 종자가 구현된다. 또한, 이종의 폴리뉴클레오티드 또는 관심 폴리뉴클레오티드의 유전자 또는 유전자 산물이 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 식물이 구현된다. 이종의 폴리뉴클레오티드 또는 관심 폴리뉴클레오티드의 발현이 상기 식물의 표현형을 변경하는 식물이 또한 구현된다. 프로모터 활성을 갖는 기능적 단편을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 단편이 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189로 이루어진 군으로부터 선택되는 뉴클레오티드 서열로부터 유도되고, 프로모터 활성을 갖는 기능적 단편이 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트가 또한 구현된다.
본 발명은 단리되거나 실질적으로 정제된 핵산 조성물을 포함한다. "단리" 또는 "정제"된 핵산 분자 또는 이의 생물학적 활성 부분에는, 재조합 기법에 의해 생성될 때 다른 세포 물질 또는 배양 배지가 실질적으로 없거나, 화학적으로 합성될 때 화학적 전구체 또는 다른 화학물질이 실질적으로 없다. "단리된" 핵산에는, 핵산이 유도되는 유기체의 게놈 DNA에서 자연적으로 핵산을 플랭킹하는(즉, 핵산의 5' 말단 및 3' 말단에 위치한) 서열(단백질 암호화 서열 포함)이 실질적으로 없다. 예를 들어, 다양한 양태에서, 단리된 핵산 분자는 핵산이 유도되는 세포의 게놈 DNA에서 자연적으로 핵산 분자를 플랭킹하는 뉴클레오티드 서열을 약 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb, 또는 0.1 kb 미만으로 함유할 수 있다. 본 발명의 서열은 각각의 전사 개시 부위를 플랭킹하는 5' 미번역 영역으로부터 단리될 수 있다.
개시된 프로모터 뉴클레오티드 서열의 단편 및 변이체도 본 발명에 포함된다. 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 프로모터 서열의 단편 및 변이체가 본 발명의 DNA 구축물에 사용될 수 있다. 본원에 사용된 용어 "단편"은 핵산 서열의 일부를 지칭한다. 프로모터 서열의 단편은 전사를 개시하는, 예컨대 항시적 방식으로 전사를 유도하는 생물학적 활성을 보유한다. 대안적으로, 혼성화 프로브로서 유용한 뉴클레오티드 서열의 단편이 생물학적 활성을 반드시 보유할 필요는 없다. 본원에 개시된 프로모터에 대한 뉴클레오티드 서열의 단편은 적어도 약 20, 25, 50, 75, 100, 125, 150, 175, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475, 500, 525, 550, 575, 600, 625, 650, 675, 700, 725, 750, 775, 800, 825, 850, 875, 900, 925, 950, 975, 1000, 1025, 1050, 1075, 1100, 1125, 1150, 1175, 1200, 1225, 1250, 1275, 1300, 1325, 1350, 1375, 1400, 1425, 1450, 1475, 1500, 1525, 1550, 1575, 1600, 1625, 1650, 1675, 1700, 1725, 1750, 1775, 1800, 1825, 1850, 1875, 1900, 1925, 1950, 1975, 2000, 2025, 2050, 2075, 2100, 2125, 2150, 2175, 2200, 2225, 2250, 2275, 2300, 2325, 2350, 2375, 2400, 2425, 2450, 2475, 2500, 2525, 2550, 2575, 2600, 2625, 2650, 2675, 2700, 2725, 2750, 2775, 2800, 2825, 2850, 2875, 2900, 2925, 2950, 2975, 3000, 3025, 3050, 3075, 3100, 3125, 3150, 3175, 3200, 3225, 3250, 3275, 3300, 3325, 3350, 3375, 3400, 3425, 3450, 3475, 3500, 3525, 3550, 3575, 3600, 3625, 3650, 3675, 3700, 3725, 3750, 3775, 3800, 3825, 3850, 3875, 3900, 3925, 3950, 3975, 4000, 4025, 4050, 4075, 4100, 4125, 4150, 4175, 4200, 4225, 4250, 4275, 4300, 4325, 4350, 4375, 4400, 4425, 4450, 4475, 4500, 4525, 4550, 4575, 4600, 4625, 4650, 4675, 4700, 4725, 4750, 4775, 4800, 4825, 4850, 4875, 4900, 4925, 4950, 4975, 5000, 5025, 5050, 5075, 5100, 5125, 5150, 5175, 5200, 5225, 5250, 5275, 5300, 5325, 5350, 5375, 5400, 5425, 5450, 5475, 5500, 5525, 5550, 5575, 5600, 5625, 5650, 5675, 5700, 5725, 5750, 5775, 5800, 5825, 5850, 5875, 5900, 5925, 5950, 5975, 6000, 6025, 6050, 6075, 6100, 6125, 6150, 6175, 6200, 또는 6225개의 뉴클레오티드 내지 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 전장의 범위일 수 있다. 프로모터의 생물학적 활성 부분은 본 발명의 프로모터 서열의 일부를 단리하고, 그 부분의 전사 활성을 평가함으로써 제조될 수 있다.
본원에 사용된 용어 "변이체"는 본원에 개시된 프로모터 서열과 실질적으로 유사한 서열을 의미한다. 변이체는 천연 폴리뉴클레오티드 내의 하나 이상의 내부 부위에서의 하나 이상의 뉴클레오티드의 결실 및/또는 첨가, 및/또는 천연 폴리뉴클레오티드의 하나 이상의 부위에서의 하나 이상의 뉴클레오티드의 치환을 포함한다. 본원에 사용된 바와 같이, "천연" 뉴클레오티드 서열은 자연발생적 뉴클레오티드 서열을 포함한다. 뉴클레오티드 서열의 경우, 자연발생적 변이체는 주지의 분자 생물학 기법을 이용하여, 예를 들어 본원에 요약된 바와 같은 중합효소 연쇄 반응(PCR) 및 혼성화 기법으로 동정될 수 있다.
변이체 뉴클레오티드 서열은 또한, 예를 들어 부위-특이적 돌연변이 유도를 이용해 생성된 것과 같은 합성적으로 유도된 뉴클레오티드 서열을 포함한다. 일반적으로, 본원에 개시된 뉴클레오티드 서열의 변이체는 기본 파라미터를 사용해 본원에 기술된 서열 정렬 프로그램으로 결정시, 해당 뉴클레오티드 서열과 적어도 40%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, to 95%, 96%, 97%, 98%, 99% 이상의 서열 동일성을 가질 것이다. 본원에 개시된 뉴클레오티드 서열의 생물학적 활성 변이체도 포함된다. 생물학적 활성 변이체는, 예를 들어 하나 이상의 뉴클레오티드 치환, 결실, 또는 삽입을 갖는 본원에 개시된 뉴클레오티드 서열의 천연 프로모터 서열을 포함한다. 프로모터 활성은 노던 블롯 분석, 전사 융합에서 얻은 리포터 활성 측정 등과 같은 기법을 이용해 측정될 수 있다. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Sambrook, et al., (1989) Molecular Cloning: A Laboratory Manual (2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.)] 참조(이하 "Sambrook"이라 함). 대안적으로, 프로모터 단편 또는 변이체의 제어하에 생성된 녹색 형광 단백질(GFP) 또는 황색 형광 단백질(YFP) 등과 같은 리포터 유전자의 수준이 측정될 수 있다. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Matz et al. (1999) Nature Biotechnology 17:969-973]; 미국 특허 번호 6,072,050; 문헌[Nagai, et al., (2002) Nature Biotechnology 20(1):87-90] 참조. 변이체 뉴클레오티드 서열은 또한, DNA 셔플링과 같은 변이원성 재조합성 절차로부터 유도된 서열을 포함한다. 이러한 절차에서, 프로모터에 대한 하나 이상의 상이한 뉴클레오티드 서열을 조작하여 새로운 프로모터를 생성할 수 있다. 이러한 방식으로, 실질적인 서열 동일성을 가지며 시험관내 또는 생체내에서 상동 재조합될 수 있는 서열 영역을 포함하는 관련 서열 폴리뉴클레오티드의 집단으로부터 재조합 폴리뉴클레오티드의 라이브러리가 생성된다. 이러한 DNA 셔플링 전략은 당업계에 알려져 있다. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Stemmer, (1994) Proc. Natl. Acad. Sci. USA 91:10747-10751; Stemmer, (1994) Nature 370:389 391; Crameri, et al., (1997) Nature Biotech. 15:436-438; Moore, et al., (1997) J. Mol. Biol. 272:336-347; Zhang, et al., (1997) Proc. Natl. Acad. Sci. USA 94:4504-4509; Crameri, et al., (1998) Nature 391:288-291]과 미국 특허 번호 5,605,793 및 5,837,458 참조.
돌연변이 유도 및 뉴클레오티드 서열 변경을 위한 방법은 당업계에 잘 알려져 있다. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Kunkel, (1985) Proc. Natl. Acad. Sci. USA 82:488-492; Kunkel, et al., (1987) Methods in Enzymol. 154:367-382]; 미국 특허 번호 4,873,192; 문헌[Walker and Gaastra, eds. (1983) Techniques in Molecular Biology (MacMillan Publishing Company, New York)], 및 거기에 인용된 참고문헌 참조.
본 발명의 뉴클레오티드 서열은 다른 유기체, 구체적으로는 다른 식물, 보다 구체적으로는 다른 외떡잎식물 또는 쌍떡잎식물로부터 해당 서열을 단리하는 데 사용될 수 있다. 이러한 방식으로, PCR, 혼성화 등과 같은 방법을 사용해, 본원에 제시된 서열과의 서열 상동성에 기초하여 이러한 서열을 동정할 수 있다. 본원에 제시된 전체 서열 또는 이들의 단편과의 서열 동일성에 기초하여 단리된 서열은 본 발명에 포함된다.
PCR 접근법에서, 임의의 관심 식물로부터 추출된 cDNA 또는 게놈 DNA로부터 해당 DNA 서열을 증폭시키기 위해 PCR 반응에 사용하기 위한 올리고뉴클레오티드 프라이머가 설계될 수 있다. PCR 프라이머 설계 및 PCR 클로닝 방법은 일반적으로 당업계에 알려져 있으며, 상기 Sambrook에 개시되어 있다. 또한, 본원에 전문이 참조로 포함되는 문헌[Innis, et al., eds. (1990) PCR Protocols: A Guide to Methods and Applications (Academic Press, New York); Innis and Gelfand, eds. (1995) PCR Strategies (Academic Press, New York); 및 Innis and Gelfand, eds. (1999) PCR Methods Manual (Academic Press, New York)] 참조. 공지된 PCR 방법은 쌍을 이룬 프라이머, 내부 프라이머, 단일 특이적 프라이머, 변성 프라이머, 유전자-특이적 프라이머, 벡터-특이적 프라이머, 부분적 불일치 프라이머 등을 이용하는 방법을 포함하나, 이에 한정되지 않는다.
혼성화 기법에서는, 알려진 뉴클레오티드 서열의 전부 또는 일부를, 선택된 유기체로부터 클로닝된 게놈 DNA 단편 또는 cDNA 단편의 집단(즉, 게놈 또는 cDNA 라이브러리)에 존재하는 다른 해당 뉴클레오티드 서열에 선택적으로 혼성화하는 프로브로서 사용한다. 혼성화 프로브는 게놈 DNA 단편, cDNA 단편, RNA 단편, 또는 기타 올리고뉴클레오티드일 수 있으며, 검출 가능한 그룹, 예컨대 32P 또는 임의의 다른 검출 가능한 마커로 표지될 수 있다. 따라서, 예를 들어 혼성화 프로브는 본 발명의 프로모터에 기초한 합성 올리고뉴클레오티드의 표지에 의해 제조될 수 있다. 혼성화 프로브의 제조 및 게놈 라이브러리의 구축 방법은 일반적으로 당업계에 알려져 있으며, 상기 Sambrook에 개시되어 있다.
예를 들어, 본원에 개시된 전체 프로모터 서열, 또는 이의 하나 이상의 부분이 해당 프로모터 서열 및 메신저 RNA에 특이적으로 혼성화할 수 있는 프로브로서 사용될 수 있다. 다양한 조건에서 특이적 혼성화를 달성하기 위해, 이러한 프로브는, 프로모터 서열 중에서 고유하고 일반적으로 약 10개 이상의 뉴클레오티드 길이 또는 약 20개 이상의 뉴클레오티드 길이인 서열을 포함한다. 이러한 프로브는 선택된 식물로부터 해당 프로모터 서열을 PCR로 증폭시키는 데 사용될 수 있다. 이 기법은 원하는 유기체로부터 추가의 코딩 서열을 단리하는 데 사용되거나, 유기체에 코딩 서열이 존재하는지 확인하기 위한 진단 분석법으로서 사용될 수 있다. 혼성화 기법은 도말된 DNA 라이브러리의 혼성화 스크리닝을 포함한다(플라크 또는 콜로니, 예를 들어 상기 Sambrook 참조).
이러한 서열의 혼성화는 엄격한 조건에서 수행될 수 있다. 용어 "엄격한 조건" 또는 "엄격한 혼성화 조건"은 프로브가 다른 서열보다 표적 서열에 더 검출 가능한 정도(예를 들어, 백그라운드에 비해 적어도 2배)로 혼성화하는 조건을 의미하는 것이다. 엄격한 조건은 서열 의존적이며 상황에 따라 다를 것이다. 혼성화 및/또는 세척 조건의 엄격성을 제어함으로써, 프로브에 100% 상보적인 표적 서열을 동정할 수 있다(상동성 프로빙). 대안적으로, 엄격 조건은 서열에서 일부 불일치를 허용하여 더 낮은 정도의 유사성이 검출되도록 조정될 수 있다(이종 프로빙). 일반적으로, 프로브는 약 1000개 미만의 뉴클레오티드 길이, 최적으로는 500개 미만의 뉴클레오티드 길이이다.
일반적으로, 엄격한 조건은, 염 농도가 pH 7.0 내지 8.3에서 약 1.5 M 미만의 Na 이온, 일반적으로는 약 0.01 내지 1.0 M의 Na 이온 농도(또는 다른 염)이고, 온도가 짧은 프로브(예를 들어, 10 내지 50개의 뉴클레오티드)의 경우 약 30℃ 이상이고 긴 프로브(예를 들어, 50개 초과의 뉴클레오티드)의 경우 약 60℃ 이상인 조건일 것이다. 엄격한 조건은 포름아미드와 같은 불안정화제의 첨가로 달성될 수도 있다. 예시적인 낮은 엄격 조건은 37℃에서 30 내지 35% 포름아미드, 1 M NaCl, 1% SDS(소듐 도데실 설페이트)의 완충액을 이용한 혼성화, 및 50 내지 55℃에서 1배 내지 2배 SSC(20배 SSC=3.0 M NaCl/0.3 M 트리소듐 시트레이트)에서의 세척을 포함한다. 예시적인 적당한 엄격 조건은 37℃에서 40 내지 45% 포름아미드, 1.0 M NaCl, 1% SDS에서의 혼성화, 및 55 내지 60℃에서 0.5배 내지 1배 SSC에서의 세척을 포함한다. 예시적인 높은 엄격 조건은 37℃에서 50% 포름아미드, 1 M NaCl, 1% SDS에서의 혼성화, 및 60 내지 65℃에서 적어도 30분 동안 0.1배 SSC에서의 최종 세척을 포함한다. 혼성화를 위한 시간은 일반적으로 약 24시간 미만, 보통 약 4 내지 약 12시간이다. 세척을 위한 시간은 적어도 평형에 도달하기에 충분한 시간일 것이다.
특이성은 일반적으로 혼성화 후 세척의 함수이며, 중요한 인자는 최종 세척액의 이온 강도 및 온도이다. DNA-DNA 하이브리드의 경우, 열 융점(Tm)은 다음의 방정식(Meinkoth and Wahl, (1984) Anal. Biochem 138:267 284)으로부터 근사될 수 있다: Tm = 81.5℃ + 16.6 (log M) + 0.41 (% GC) - 0.61 (% form) - 500/L; 식에서 M은 1가 양이온의 몰 농도이고, % GC는 DNA 중의 구아노신과 시토신 뉴클레오티드의 백분율이고, % form은 혼성화 용액 중의 포름아미드의 백분율이고, L은 염기쌍에서의 하이브리드의 길이이다. Tm은 상보적인 표적 서열의 50%가 완벽하게 일치하는 프로브에 혼성화되는 (정의된 이온 강도 및 pH에서의) 온도이다. Tm은 1%의 불일치마다 약 1℃ 감소하므로, Tm, 혼성화, 및/또는 세척 조건은 원하는 동일성의 서열에 혼성화되도록 조정될 수 있다. 예를 들어, 90% 동일성을 갖는 서열이 요구되는 경우, Tm은 10℃ 감소될 수 있다. 일반적으로, 엄격한 조건은 정의된 이온 강도 및 pH에서 특정 서열 및 그 보체에 대한 Tm보다 약 5℃ 낮도록 선택된다. 그러나, 매우 엄격한 조건은 Tm보다 1, 2, 3, 또는 4℃ 낮은 온도에서의 혼성화 및/또는 세척을 사용할 수 있고, 적당히 엄격한 조건은 Tm보다 6, 7, 8, 9, 또는 10℃ 낮은 온도에서의 혼성화 및/또는 세척을 사용할 수 있고, 낮은 엄격 조건은 Tm보다 11, 12, 13, 14, 15, 또는 20℃ 낮은 온도에서의 혼성화 및/또는 세척을 사용할 수 있다. 방정식, 혼성화와 세척 조성, 및 원하는 Tm을 사용할 때, 당업자는 혼성화 및/또는 세척액의 엄격성의 변화가 본질적으로 기술되어 있음을 이해할 것이다. 원하는 불일치도에 따라 Tm이 45℃(수용액) 또는 32℃(포름아미드 용액) 미만이 되는 경우, 더 높은 온도가 사용될 수 있도록 SSC 농도를 증가시키는 것이 바람직하다. 핵산의 혼성화에 대한 광범위한 가이드는, 본원에 전문이 참조로 포함되는 문헌[Tijssen, (1993) Laboratory Techniques in Biochemistry and Molecular Biology--Hybridization with Nucleic Acid Probes, Part I, Chapter 2 (Elsevier, New York); 및 Ausubel, et al., eds. (1995) Current Protocols in Molecular Biology, Chapter 2 (Greene Publishing and Wiley-Interscience, New York)]에서 확인된다. 또한, 상기 Sambrook 참조. 따라서, 엄격한 조건에서 본원에 개시된 프로모터 서열 또는 이들의 단편에 혼성화되고 프로모터 활성을 갖는 단리된 서열은 본 발명에 포함된다.
일반적으로, 본원에 개시된 프로모터 서열에 혼성화되고 프로모터 활성을 갖는 서열은, 개시된 서열과 적어도 40% 내지 50%의 상동성, 약 60%, 70%, 80%, 85%, 90%, 95% 내지 98% 이상의 상동성을 가질 것이다. 즉, 서열의 서열 유사성은 적어도 약 40% 내지 50%, 약 60% 내지 70%, 및 약 80%, 85%, 90%, 95% 내지 98%의 서열 유사성을 공유하는 범위일 수 있다.
다음의 용어들은 둘 이상의 핵산과 폴리뉴클레오티드 간의 서열 관계를 설명하는 데 사용된다: (a) "기준 서열", (b) "비교창", (c) "서열 동일성", (d) "서열 동일성 백분율", 및 (e) "실질적 동일성".
본원에 사용된 바와 같이, "기준 서열"은 서열 비교의 기준으로서 사용되는 정의된 서열이다. 기준 서열은, 예를 들어 전장 cDNA 또는 유전자 서열 또는 완전한 cDNA 또는 유전자 서열의 세그먼트로서, 특정 서열의 서브세트 또는 전체일 수 있다.
본원에 사용된 바와 같이, "비교창"은 폴리뉴클레오티드 서열의 연속적인 특정 세그먼트를 지칭하며, 비교창 내의 폴리뉴클레오티드 서열은 (삽입 또는 결실을 포함하지 않는) 기준 서열과 비교하여 두 서열의 최적 정렬을 위한 삽입 또는 결실(즉, 갭)을 포함할 수 있다. 일반적으로, 비교창은 20개 이상의 연속 뉴클레오티드 길이이며, 선택적으로 30, 40, 50, 100개 이상의 길이일 수 있다. 당업자는 폴리뉴클레오티드 서열에 갭이 포함됨으로 인한 기준 서열과의 높은 유사성을 피하기 위해 일반적으로 갭 페널티가 도입되어 일치의 수에서 제외된다는 것을 이해한다.
비교를 위한 서열 정렬 방법은 당업계에 잘 알려져 있다. 따라서, 임의의 두 서열 간의 서열 동일성 백분율은 수학적 알고리즘을 사용하여 결정될 수 있다. 이러한 수학적 알고리즘의 비제한적인 예는, 본원에 전문이 참조로 포함되는 문헌[Myers and Miller, (1988) CABIOS 4:11-17]의 알고리즘; 문헌[Smith, et al., (1981) Adv. Appl. Math. 2:482]의 알고리즘; 문헌[Needleman and Wunsch, (1970) J. Mol. Biol. 48:443-453]의 알고리즘; 문헌[Pearson and Lipman, (1988) Proc. Natl. Acad. Sci. 85:2444-2448]의 알고리즘; 문헌[Karlin and Altschul, (1993) Proc. Natl. Acad. Sci. USA 90:5873-5877]에서 수정된 문헌[Karlin and Altschul, (1990) Proc. Natl. Acad. Sci. USA 872:264]의 알고리즘이다.
이러한 수학적 알고리즘의 컴퓨터 구현은 서열 동일성을 결정하기 위해 서열의 비교에 이용될 수 있다. 이러한 구현은 PC/Gene 프로그램의 CLUSTAL(Intelligenetics에서 입수 가능, Mountain View, Calif.); ALIGN 프로그램(버전 2.0) 및 GCG Wisconsin Genetics Software Package®, 버전 10의 GAP, BESTFIT, BLAST, FASTA 및 TFASTA(Accelrys Inc.에서 입수 가능, 9685 Scranton Road, San Diego, Calif., USA)를 포함하나, 이에 한정되지 않는다. 이러한 프로그램을 이용한 정렬은 기본 파라미터를 사용하여 수행될 수 있다. CLUSTAL 프로그램은, 본원에 전문이 참조로 포함되는 문헌[Higgins, et al., (1988) Gene 73:237-244; Higgins, et al., (1989) CABIOS 5:151-153; Corpet, et al., (1988) Nucleic Acids Res. 16:10881-90; Huang, et al., (1992) CABIOS 8:155-65; 및 Pearson, et al., (1994) Meth. Mol. Biol. 24:307-331]에 잘 설명되어 있다. ALIGN 프로그램은 상기 문헌[Myers and Miller, (1988)]의 알고리즘을 기반으로 한다. ALIGN 프로그램으로 아미노산 서열을 비교할 때 PAM120 가중치 잔기 표(weight residue table), 12의 갭 길이 페널티, 및 4의 갭 페널티를 사용할 수 있다. 본원에 전문이 참조로 포함되는 문헌[Altschul, et al., (1990) J. Mol. Biol. 215:403]의 BLAST 프로그램은 상기 문헌[Karlin and Altschul, (1990)]의 알고리즘을 기반으로 한다. 본 발명의 단백질을 암호화하는 뉴클레오티드 서열에 상동적인 뉴클레오티드 서열을 얻기 위해 BLASTN 프로그램(점수=100, 단어 길이=12)으로 BLAST 뉴클레오티드 검색을 수행할 수 있다. 본 발명의 단백질 또는 폴리펩티드에 상동적인 아미노산 서열을 얻기 위해 BLASTX 프로그램(점수=50, 단어 길이=3)으로 BLAST 단백질 검색을 수행할 수 있다. 비교를 위한 갭 포함 정렬을 얻기 위해, 본원에 전문이 참조로 포함된 문헌[Altschul, et al., (1997) Nucleic Acids Res. 25:3389]에 기재된 바와 같이 Gapped BLAST(BLAST 2.0)를 이용할 수 있다. 대안적으로, 분자 사이의 먼 관계를 감지하는 반복 검색을 수행하기 위해 PSI-BLAST(BLAST 2.0)를 사용할 수 있다. 상기 문헌[Altschul, et al., (1997)] 참조. BLAST, Gapped BLAST, PSI-BLAST를 이용할 때, 각각의 프로그램(예를 들어, 뉴클레오티드 서열의 경우 BLASTN, 단백질의 경우 BLASTX)의 기본 파라미터를 사용할 수 있다. 미국 국립생물공학정보센터의 웹 사이트 ncbi.nlm.nih.gov 참조. 정렬은 검사에 의해 수동으로 수행될 수도 있다.
달리 명시되지 않는 한, 본원에 제공된 서열 동일성/유사성 값은 하기 파라미터를 사용하여, GAP 버전 10을 이용해 얻은 값을 지칭한다. 50의 갭 가중치와 3의 길이 가중치를 사용한 뉴클레오티드 서열에 대한 동일성%와 유사성%, 및 nwsgapdna.cmp 점수 매트릭스; 8의 가중치 및 2의 길이 가중치를 사용한 아미노산 서열에 대한 동일성%와 유사성%, 및 BLOSUM62 점수 매트릭스; 또는 이들의 임의의 동등한 프로그램. 본원에 사용된 바와 같이, "동등한 프로그램"은 문제가 되는 임의의 두 서열에 대해, GAP 버전 10에 의해 생성된 해당 정렬과 비교할 때 동일한 뉴클레오티드 또는 아미노산 잔기 일치 및 동일한 서열 동일성 백분율을 갖는 정렬을 생성하는 임의의 서열 비교 프로그램이다.
GAP 프로그램은 상기 문헌[Needleman and Wunsch]의 알고리즘을 사용하여, 일치의 수를 최대화하고 갭의 수를 최소화하는 2개의 완전한 서열의 정렬을 찾는다. GAP는 가능성 있는 모든 정렬 및 갭 위치를 고려하여, 일치 염기의 수가 가장 많고 갭이 가장 적은 정렬을 생성한다. 이는 일치 염기의 단위에서 갭 생성 페널티 및 갭 확장 페널티를 제공할 수 있다. GAP는 삽입되는 각각의 갭에 대해 일치의 갭 생성 페널티 수의 이득을 만들어야 한다. 0보다 큰 갭 확장 페널티가 선택되는 경우, GAP는 삽입되는 각각의 갭에 대해 추가로 갭의 길이와 갭 확장 페널티의 곱의 이득을 만들어야 한다. 단백질 서열에 대한 GCG Wisconsin Genetics Software Package®의 버전 10에서의 기본 갭 생성 페널티 값과 갭 확장 페널티 값은 각각 8과 2이다. 뉴클레오티드 서열의 경우, 기본 갭 확장 페널티는 3인데 반해 기본 갭 생성 페널티는 50이다. 갭 생성 및 갭 확장 페널티는 0 내지 200으로 이루어진 정수 군으로부터 선택되는 정수로 표현될 수 있다. 따라서, 예를 들어 갭 생성 및 갭 확장 페널티는 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65 이상일 수 있다.
GAP는 최적 정렬 계열 중 하나의 멤버를 나타낸다. 이 계열의 멤버가 많을 수 있지만 더 좋은 품질을 갖는 다른 멤버는 없다. GAP는 정렬을 위한 4가지 성능 지수인 품질, 비율, 동일성, 및 유사성을 표시한다. 품질은 서열을 정렬하기 위해 최대화되는 측정 기준이다. 비율은 품질을 더 짧은 세그먼트에서의 염기의 수로 나눈 값이다. 동일성 백분율은 실제로 일치하는 기호의 백분율이다. 유사성 백분율은 실제로 유사한 기호의 백분율이다. 갭의 맞은편에 있는 기호는 무시된다. 한 쌍의 기호에 대한 점수 매트릭스 값이 유사성 임계값인 0.50 이상이면 유사성 점수가 매겨진다. GCG Wisconsin Genetics Software Package®의 버전 10에 사용되는 점수 매트릭스는 BLOSUM62이다(본원에 전문이 참조로 포함되는 문헌[Henikoff and Henikoff, (1989) Proc. Natl. Acad. Sci. USA 89:10915] 참조).
본원에 사용된 바와 같이, 2개의 핵산 또는 폴리펩티드 서열과 관련하여 "서열 동일성" 또는 "동일성"은 특정 비교창에서 최대 상응도로 정렬되는 경우 동일한 두 서열 내의 잔기를 의미한다. 단백질과 관련하여 서열 동일성 백분율이 사용되는 경우, 동일하지 않은 잔기 위치는, 아미노산 잔기가 화학적 특성(예를 들어, 전하량 또는 소수성)이 유사한 다른 아미노산 잔기로 치환되어 분자의 기능적 특성을 변화시키지 않는 보존적 아미노산 치환에 의해 종종 상이한 것으로 인식된다. 서열 간에 보존적 치환이 다른 경우, 치환의 보존적 성질을 교정하도록 서열 동일성 백분율이 상향 조정될 수 있다. 이러한 보존적 치환이 다른 서열을 "서열 유사성" 또는 "유사성"을 갖는다고 한다. 이러한 조정을 위한 수단은 당업자에게 잘 알려져 있다. 일반적으로, 이는 보존적 치환을 완전 불일치가 아니라 부분적 불일치로 점수를 매김으로써 서열 동일성 백분율을 증가시키는 것을 수반한다. 따라서, 예를 들어 동일한 아미노산의 점수가 1이고 비보존적 치환의 점수가 0인 경우, 보존적 치환의 점수는 0과 1 사이이다. 보존적 치환의 점수는, 예를 들어 PC/GENE 프로그램(Intelligenetics, Mountain View, Calif.)에서 구현된 바와 같이 계산된다.
본원에 사용된 바와 같이, "서열 동일성 백분율"은 비교창에서 최적으로 정렬된 2개의 서열을 비교하여 결정된 값을 의미하며, 비교창 내의 폴리뉴클레오티드 서열의 부분은 (삽입 또는 결실을 포함하지 않는) 기준 서열과 비교하여 두 서열의 최적 정렬을 위한 삽입 또는 결실(즉, 갭)을 포함할 수 있다. 백분율은, 동일한 핵산 염기 또는 아미노산 잔기가 두 서열 모두에서 발생하는 위치의 수를 결정하여 일치하는 위치의 수를 산출하고, 일치하는 위치의 수를 비교창 내의 위치의 총 수로 나누고, 그 결과에 100을 곱하여 서열 동일성 백분율을 산출함으로써 계산된다.
폴리뉴클레오티드 서열의 "실질적 동일성"이란 용어는, 폴리뉴클레오티드가 표준 파라미터를 사용하여 정렬 프로그램을 이용해 기준 서열과 비교할 때 적어도 70%, 최적으로는 적어도 80%, 더 최적으로는 적어도 90%, 가장 최적으로는 적어도 95%의 서열 동일성을 갖는 서열을 포함함을 의미한다. 당업자는 코돈 변성, 아미노산 유사성, 리딩 프레임 포지셔닝 등을 고려하여, 2개의 뉴클레오티드 서열에 의해 암호화되는 단백질의 상응하는 동일성을 결정하기 위해 이들 값이 적절히 조정될 수 있음을 인식할 것이다. 이러한 목적을 위한 아미노산 서열의 실질적 동일성은 일반적으로, 적어도 60%, 70%, 80%, 90%, 및 적어도 95%의 서열 동일성을 의미한다.
뉴클레오티드 서열이 실질적으로 동일하다는 또 다른 표시는 두 분자가 엄격한 조건에서 서로 혼성화되는 경우이다. 일반적으로, 엄격한 조건은 정의된 이온 강도 및 pH에서 특정 서열에 대한 Tm보다 약 5℃ 낮도록 선택된다. 그러나, 엄격한 조건은 본원에 달리 규정된 바와 같이, 원하는 엄격성의 정도에 따라 Tm보다 약 1℃ 내지 약 20℃ 낮은 범위의 온도를 포함한다. 엄격한 조건에서 서로 혼성화되지 않는 핵산은, 이들이 암호화하는 폴리펩티드가 실질적으로 동일한 경우, 여전히 실질적으로 동일하다. 이는, 예를 들어 유전자 코드에 의해 허용되는 최대 코돈 변성을 사용하여 핵산의 카피가 생성되는 경우에 발생할 수 있다. 2개의 핵산 서열이 실질적으로 동일하다는 하나의 표시는 제1 핵산에 의해 암호화된 폴리펩티드가 제2 핵산에 의해 암호화된 폴리펩티드와 면역학적으로 교차 반응성인 경우이다.
본원에 개시된 조직-선호적 프로모터, 뿐만 아니라 이들의 변이체 및 단편은 식물의 유전자 조작에 유용하다(예를 들어 관심 표현형을 발현시키기 위해 형질전환 또는 유전자이식 식물을 제조하는 데 유용하다). 본원에 사용된 용어 "형질전환 식물" 및 "유전자이식 식물"은 게놈 내에 이종 폴리뉴클레오티드를 포함하는 식물을 지칭한다. 일반적으로, 이종 폴리뉴클레오티드는 폴리뉴클레오티드가 대대로 전달되도록 유전자이식 또는 형질전환 식물의 게놈 내에 안정적으로 통합된다. 이종 폴리뉴클레오티드는 단독으로 또는 재조합 DNA 구축물의 일부로서 게놈 내에 통합될 수 있다. 본원에 사용된 용어 "유전자이식"은 초기에 그렇게 변경된 유전자이식체뿐만 아니라 초기의 유전자이식체로부터 유성 교배 또는 무성 번식에 의해 생성된 것들을 포함하여, 이종 핵산의 존재에 의해 유전형이 변경된 임의의 세포, 세포주, 캘러스, 조직, 식물 부분 또는 식물을 포함하는 것으로 이해되어야 한다.
유전자이식 "이벤트"는 관심 유전자를 포함하는 핵산 발현 카세트를 포함하는 이종 DNA 구축물을 이용한 식물 세포의 형질전환, 전달된 유전자를 식물의 게놈에 삽입함으로 인한 식물 집단의 재생, 및 특정 게놈 위치에 삽입되는 것을 특징으로 하는 식물의 선별에 의해 생성된다. 이벤트는 삽입된 유전자의 발현을 표현형적인 특징으로 한다. 유전자 수준에서, 이벤트는 식물의 유전자 구성의 일부이다. 용어 "이벤트"는 또한, 형질전환체와 다른 식물 간의 유성 교배에 의해 생성된 자손을 지칭하며, 여기서 자손은 이종 DNA를 포함한다.
용어 "식물"은 전체 식물, 식물 기관, 식물 조직, 종자, 식물 세포, 종자 및 그 자손을 지칭한다. 식물 세포는 종자 세포, 현탁 배양물, 배아, 분열부, 캘러스 조직, 잎, 뿌리, 싹, 배우체, 포자체, 꽃가루, 및 미포자를 제한 없이 포함한다.
식물 부분은 분화 및 미분화 조직(뿌리, 줄기, 싹, 잎, 꽃가루, 종자, 종양 조직 및 다양한 형태의 세포 및 배양물(예컨대, 단일 세포, 원형질체, 배아, 및 캘러스 조직)을 포함하나, 이에 한정되지 않음)을 포함한다. 식물 조직은 식물에, 또는 식물 기관, 조직, 또는 세포 배양물에 있을 수 있다.
본 발명은 또한, 본원에 개시된 임의의 방법 또는 조성물에 의해 얻은 식물을 포함한다. 본 발명은 또한, 본원에 개시된 임의의 방법 또는 조성물에 의해 얻은 식물의 종자를 포함한다. 용어 "식물"은 전체 식물, 식물 기관(예컨대, 잎, 줄기, 뿌리 등), 식물 조직, 식물 세포, 식물 부분, 종자, 육아, 배아, 및 그 자손을 지칭한다. 식물 세포는 분화 또는 미분화 세포(예를 들어, 캘러스, 미분화 캘러스, 미숙배, 성숙배, 미숙 접합배, 미숙 자엽, 배축, 현탁 배양 세포, 원형질체, 잎, 잎 세포, 뿌리 세포, 체관부 세포, 및 꽃가루)일 수 있다. 식물 세포는 종자 세포, 현탁 배양물, 외식편, 미숙배, 배아, 접합배, 체세포배, 배발생 캘러스, 분열조직, 체세포 분열조직, 기관형성 캘러스, 원형질체, 성숙 이삭-유도 종자로부터 유도된 배아, 엽저, 성숙 식물의 잎, 잎끝, 미숙 꽃, 수술, 미숙 이삭, 수염, 자엽, 미숙 자엽, 배축, 분열부, 캘러스 조직, 잎 세포, 줄기 세포, 뿌리 세포, 싹 세포, 배우체, 포자체, 꽃가루, 및 미포자를 제한 없이 포함한다. 식물 부분은 분화 및 미분화 조직(뿌리, 줄기, 싹, 잎, 꽃가루, 종자, 종양 조직, 및 배양물의 다양한 형태의 세포(예컨대, 단일 세포, 원형질체, 배아, 및 캘러스 조직)을 포함하나, 이에 한정되지 않음)을 포함한다. 식물 조직은 식물에, 또는 식물 기관, 조직, 또는 세포 배양물에 있을 수 있다. 곡물은 종의 성장 또는 생식 이외의 목적을 위해 상업적 재배업자에 의해 생산되는 성숙 종자를 의미하는 것이다. 재생 식물의 자손, 변이체, 및 돌연변이체가 도입된 폴리뉴클레오티드를 포함하는 한, 이들 자손, 변이체, 및 돌연변이체도 본 발명의 범위 내에 포함된다.
본 발명은 임의의 식물 종(외떡잎식물 및 쌍떡잎식물(옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 및 배추속을 포함하나, 이에 한정되지 않음)을 포함하나, 이에 한정되지 않음)의 형질전환에 사용될 수 있다. 외떡잎식물은 보리, 옥수수(콘), 밀렛(예컨대, 펄 밀렛(Pennisetum glaucum), 프로소 밀렛(Panicum miliaceum), 조(Setaria italica), 손가락조(Eleusine coracana)), 귀리, 벼, 호밀, Setaria 종, 수수, 라이밀, 또는 밀, 또는 잎과 줄기 작물(대나무, 마람 그라스, 왕포아풀, 갈대, 독보리, 사탕수수를 포함하나, 이에 한정되지 않음), 잔디, 관상용 그라스, 및 기타 그라스, 예컨대 스위치그라스 및 터프 그라스를 포함하나, 이에 한정되지 않는다. 대안적으로, 본 발명에 사용되는 쌍떡잎식물은 케일, 콜리플라워, 브로콜리, 겨자, 양배추, 완두, 클로버, 알팔파, 잠두, 토마토, 땅콩, 카사바, 대두, 카놀라, 해바라기, 잇꽃, 담배, 애기장대, 또는 목화를 포함하나, 이에 한정되지 않는다.
고등 식물, 예를 들어 속씨식물 및 겉씨식물 강이 본 발명에 사용될 수 있다. 본 발명에 유용한 적합한 종의 식물은 쥐꼬리망초과, 부추과, 알스트로에메리아과, 수선화과, 협죽도과, 야자나무과, 국화과, 매자나무과, 홍목과, 십자화과, 파인애플과, 삼과, 석죽과, 개비자나무과, 명아주과, 콜키쿰과, 박과, 마과, 마황과, 코카나무과, 대극과, 콩과, 꿀풀과, 아마과, 석송과, 아욱과, 멜란티움과, 파초과, 도금양과, 니사나무과, 양비귀과, 소나무과, 질경이과, 벼과, 장미과, 꼭두서니과, 버드나무과, 무환자나무과, 가지과, 주목과, 차나무과, 및 포도과 식물일 수 있다. 닥풀(Abelmoschus) 속, 침엽수(Abies) 속, 관상수(Acer) 속, 겨이삭(Agrostis) 속, 부추(Allium) 속, 알스트로메리아(Alstroemeria) 속, 아나나스(Ananas,) 속, 천심련(Andrographis) 속, 쇠풀(Andropogon) 속, 쑥(Artemisia) 속, 물대(Arundo) 속, 아트로파(Atropa) 속, 매자나무(Berberis) 속, 베타(Beta) 속, 빅사(Bixa) 속, 브라시카(Brassica) 속, 칼렌둘라(Calendula) 속, 카멜리아(Camellia) 속, 캠프노테카(Camptotheca) 속, 칸나비스(Cannabis) 속, 캡시쿰(Capsicum) 속, 카르타무스(Carthamus) 속, 카타란투스(Catharanthus) 속, 세팔로탁수스(Cephalotaxus) 속, 크리산테눔(Chrysanthemum) 속, 신코나(Cinchona) 속, 시트룰러스(Citrullus) 속, 코페아(Coffea) 속, 콜키쿰(Colchicum) 속, 콜레우스(Coleus) 속, 쿠쿠미스(Cucumis) 속, 쿠쿠비타(Cucurbita) 속, 시노돈(Cynodon) 속, 다투라(Datura) 속, 디안투스(Dianthus) 속, 디지탈리스(Digitalis) 속, 디오스코레아(Dioscorea) 속, 엘라에이스(Elaeis) 속, 에페드라(Ephedra) 속, 에리안투스(Erianthus) 속, 에리톡실룸(Erythroxylum) 속, 에페드라(Eucalyptus) 속, 페스투카(Festuca) 속, 프라가리아(Fragaria) 속, 갈란투스(Galanthus) 속, 글리신(Glycine) 속, 고십피움(Gossypium) 속, 헬리안투스(Helianthus) 속, 헤비아(Hevea) 속, 호르데움(Hordeum) 속, 히오스시아무스(Hyoscyamus) 속, 자트로파(Jatropha) 속, 락투카(Lactuca) 속, 리눔(Linum) 속, 롤리움(Lolium) 속, 루피누스(Lupinus) 속, 리코페드시콘(Lycopersicon) 속, 리코포디움(Lycopodium) 속, 만니핫(Manihot) 속, 메디카고(Medicago) 속, 멘타(Mentha) 속, 미스칸투스(Miscanthus) 속, 무사(Musa) 속, 니코티아나(Nicotiana) 속, 오리자(Oryza,) 속, 파니쿰(Panicum) 속, 파파비르(Papaver) 속, 파르테니움(Parthenium) 속, 펜니세툼(Pennisetum) 속, 페투니아(Petunia) 속, 팔라리스(Phalaris) 속, 플레움(Phleum) 속, 피누스(Pinus) 속, 포아(Poa) 속, 포인세티아(Poinsettia) 속, 포퓰러스(Populus) 속, 라우울피아(Rauwolfia) 속, 리시누스(Ricinus) 속, 로사(Rosa) 속, 사카룸(Saccharum) 속, 살릭스(Salix) 속, 산구이나리아(Sanguinaria) 속, 스코폴리아(Scopolia) 속, 호밀(Secale) 속, 가지(Solanum) 속, 수수(Sorghum) 속, 스파르티나(Spartina) 속, 스피나세아(Spinacea) 속, 타나세툼(Tanacetum) 속, 주목(Taxus) 속, 테오브로마(Theobroma) 속, 라이밀(Triticosecale) 속, 트리티쿰(Triticum) 속, 유니올라(Uniola) 속, 여로(Veratrum) 속, 빈카(Vinca) 속, 포도나무(Vitis) 속 및 옥수수(Zea) 속의 구성원 식물이 본 발명의 방법에 사용될 수 있다.
농업, 원예, 바이오매스 생산(액체 연료 분자 및 기타 화학물질 생산용), 및/또는 임업에 중요하거나 도움이 되는 식물이 본 발명의 방법에 사용될 수 있다. 비제한적인 예는, 예를 들어 Panicum virgatum(스위치그라스), Miscanthus giganteus(억새), Saccharum 종(사탕수수, 에너지케인), Populus balsamifera(포플러), 목화(Gossypium barbadense, Gossypium hirsutum), Helianthus annuus(해바라기), Medicago sativa(알팔파), Beta vulgaris(사탕무), 수수(Sorghum bicolor, Sorghum vulgare), Erianthus 종, Andropogon gerardii(큰 푸른줄기), Pennisetum purpureum(코끼리풀), Phalaris arundinacea(리이드케너리그라스), Cynodon dactylon(우산잔디), Festuca arundinacea(톨페스큐), Spartina pectinata(프레리 코드-그라스), Arundo donax(물대), Secale cereale(호밀), Salix 종(버드나무), Eucalyptus 종(유칼립투스, E. grandis(및 이의 잡종, "urograndis"로 알려짐), E. globulus, E. camaldulensis, E. tereticornis, E.viminalis, E. nitens, E. saligna 및 E. urophylla 포함), Triticosecale 종(밀속 - 밀 X 호밀), 대나무, Carthamus tinctorius(잇꽃), Jatropha curcas(자트로파), Ricinus communis(아주까리), Elaeis guineensis(야자), Linum usitatissimum(아마), Manihot esculenta(카사바), Lycopersicon esculentum(토마토), Lactuca sativa(상추), Phaseolus vulgaris(껍질콩), Phaseolus limensis(리마콩), Lathyrus 종(완두콩), Musa paradisiaca(바나나), Solanum tuberosum(감자), Brassica 종(B. napus(카놀라), B. rapa, B. juncea), Brassica oleracea(브로콜리, 콜리플라워, 미니양배추), Camellia sinensis(차), Fragaria ananassa(딸기), Theobroma cacao(코코아), Coffea arabica(커피), Vitis vinifera(포도), Ananas comosus(파인애플), Capsicum annum(고추 및 피망), Arachis hypogaea(땅콩), Ipomoea batatus(고구마), Cocos nucifera(코코넛), Citrus 종(감귤 나무), Persea americana(아보카도), 무화과(Ficus casica), 구아바(Psidium guajava), 망고(Mangifera indica), 올리브(Olea europaea), Carica papaya(파파야), Anacardium occidentale(캐슈), Macadamia integrifolia(마카디아 나무), Prunus amygdalus(아몬드), Allium cepa(양파), Cucumis melo(머스크멜론), Cucumis sativus(오이), Cucumis cantalupensis(칸탈루프), Cucurbita maxima(호박), Cucurbita moschata(호박), Spinacea oleracea(시금치), Citrullus lanatus(수박), Abelmoschus esculentus(오크라), Solanum melongena(가지), Cyamopsis tetragonoloba(구아콩), Ceratonia siliqua(구주콩), Trigonella foenum-graecum(호로파), Vigna radiata(녹두), Vigna unguiculata(동부), Vicia faba(잠두), Cicer arietinum(병아리콩), Lens culinaris(렌즈콩), Papaver somniferum(양귀비), Papaver orientale, Taxus baccata, Taxus brevifolia, Artemisia annua, Cannabis sativa, Camptotheca acuminate, Catharanthus roseus, Vinca rosea, Cinchona officinalis, Colchicum autumnale, Veratrum californica., Digitalis lanata, Digitalis purpurea, Dioscorea 종, Andrographis paniculata, Atropa belladonna, Datura stomonium, Berberis 종, Cephalotaxus 종, Ephedra sinica, Ephedra 종, Erythroxylum coca, Galanthus wornorii, Scopolia 종, Lycopodium serratum(뱀톱), Lycopodium 종, Rauwolfia serpentina, Rauwolfia 종, Sanguinaria canadensis, Hyoscyamus 종, Calendula officinalis, Chrysanthemum parthenium, Coleus forskohlii, Tanacetum parthenium, Parthenium argentatum(구아율), Hevea 종(고무), Mentha spicata(민트), Mentha piperita(민트), Bixa orellana(아치오테), Alstroemeria 종, Rosa 종(장미), Rhododendron 종(아젤리아), Macrophylla hydrangea(수국), Hibiscus rosasanensis(하이비스커스), Tulipa 종(튤립), Narcissus 종(수선화), Petunia hybrida (피튜니아), Dianthus caryophyllus(카네이션), Euphorbia pulcherrima(포인세티아), chrysanthemum, Nicotiana tabacum(담배), Lupinus albus(루핀), Uniola paniculata(귀리), 벤트 그라스(Agrostis 종), Populus tremuloides(사시나무), Pinus 종(소나무), Abies 종(미송), Acer 종(단풍나무), Hordeum vulgare(보리), Poa pratensis(왕포아풀), Lolium 종(독보리), Phleum pratense(티모시), 및 침엽수를 포함한다.
침엽수가 본 발명에 사용될 수 있으며, 예를 들어, 소나무, 예컨대 테다 소나무(Pinus taeda), 슬래시 소나무(Pinus elliotii), 폰데로사 소나무(Pinus ponderosa), 로지폴 소나무(Pinus contorta), 및 몬테레이 소나무(Pinus radiata); 미송(Pseudotsuga menziesii); 캐나다 솔송나무(Tsuga canadensis); 미국 솔송나무(Tsuga heterophylla); 마운틴 헴록(Tsuga mertensiana); 미국 낙엽송 또는 낙엽송(Larix occidentalis); 시트카 가문비나무(Picea glauca); 거대 가문비나무(Sequoia sempervirens); 전나무, 예컨대 유럽 전나무(Abies amabilis) 및 발삼 전나무(Abies balsamea); 및 삼나무, 예컨대 미국 삼나무(Thuja plicata) 및 알래스카 측백나무(Chamaecyparis nootkatensis)를 포함한다.
터프 그라스가 본 발명에 사용될 수 있으며, 새포아풀(Poa annua); 쥐보리(Lolium multiflorum); 좀포아풀(Poa compressa); 콜로니얼 벤트그라스(Agrostis tenuis); 크리핑 벤트그라스(Agrostis palustris); 마초풀(Agropyron desertorum); 페어웨이 휘트그라스(Agropyron cristatum); 하드훼스큐(Festuca longifolia); 왕포아풀(Poa pratensis); 오리새(Dactylis glomerata); 다년생 독보리(Lolium perenne); 레드훼스큐(Festuca rubra); 흰겨이삭(Agrostis alba); 큰새포아풀(Poa trivialis); 김의털(Festuca ovina); 스무스부롬그라스(Bromus inermis); 티모시(Phleum pratense); 벨벳 벤트그라스(Agrostis canina); 위핑 알카리그라스(Puccinellia distans); 웨스턴 휘트그라스(Agropyron smithii); 성어거스틴 그라스(Stenotaphrum secundatum); 조이시아 그라스(Zoysia spp.); 바히아 그라스(Paspalum notatum); 카펫 그라스(Axonopus affinis); 센티페드 그라스(Eremochloa ophiuroides); 키쿠유 그라스(Pennisetum clandesinum); 씨소어 파스팔름(Paspalum vaginatum); 블루 그라마(Bouteloua gracilis); 버팔로 그라스(Buchloe dactyloids); 시데오에이트 그라마(Bouteloua curtipendula)를 포함하나, 이에 한정되지 않는다.
특정 양태에서, 본 발명의 식물은 경작 식물(예를 들어, 콘, 알팔파, 해바라기, 배추속, 대두, 목화, 잇꽃, 땅콩, 벼, 수수, 밀, 밀렛, 담배 등)이다. 본 발명에 유용한 다른 식물은 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 및 멜론을 포함한다.
본 발명의 프로모터 서열에 의해 발현되는 추가의 이종 코딩 서열, 이종 폴리뉴클레오티드, 및 관심 폴리뉴클레오티드가 식물의 표현형을 변화시키기 위해 사용될 수 있다. 식물의 유전자 발현을 수정하는 것, 식물의 병원체 또는 곤충 방어 메커니즘을 변경하는 것, 제초제에 대한 식물 내성을 증가시키는 것, 환경 스트레스에 대응하도록 식물 발육을 변경하는 것, 염, 온도(더위와 추위), 가뭄 등에 대한 식물의 반응을 조절하는 것을 포함하여, 표현형의 다양한 변화가 관심의 대상이다. 이러한 결과는 적절한 유전자 산물을 포함하는 이종의 관심 뉴클레오티드 서열을 발현시킴으로써 달성될 수 있다. 특정 양태에서, 이종의 관심 뉴클레오티드 서열은, 식물 또는 식물 부분에서 발현 수준이 증가되는 내인성 식물 서열이다. 하나 이상의 내인성 유전자 산물, 특히 호르몬, 수용체, 신호전달 분자, 효소, 운반체, 또는 보조 인자의 발현을 변경하거나, 식물의 영양분 섭취에 영향을 주어 결과를 달성할 수 있다. 본원에 개시된 프로모터에 의해 제공되는 조직-선호적 프로모터는 유전자 산물 발현을 변경할 수 있다. 이러한 변화는 형질전환 식물의 표현형을 변화시킨다. 특정 양태에서, 발현 패턴이 조직-선호적이므로, 발현 패턴은 여러 유형의 스크리닝에 유용하다.
본 발명에 대한 관심 뉴클레오티드 서열의 일반적인 범주는, 예를 들어 징크 핑거와 같은 정보 관련 유전자, 키나제와 같은 신호전달 관련 유전자, 및 열충격 단백질과 같은 하우스키핑 관련 유전자를 포함한다. 이식유전자의 보다 구체적인 범주는, 예를 들어 작물학적 특성, 곤충 저항성, 병해 저항성, 제초제 저항성, 환경 스트레스 저항성(추위, 염, 가뭄 등에 대한 내성 변경), 및 곡물 특성에 대한 중요한 형질을 암호화하는 유전자를 포함한다. 이식유전자의 또 다른 범주는 식물 및 기타 진핵 생물뿐만 아니라 원핵 생물의 효소, 보조인자, 및 호르몬과 같은 외인성 산물의 발현을 유도하는 유전자를 포함한다. 임의의 관심 유전자 또는 폴리뉴클레오티드는 본 발명의 프로모터에 작동 가능하게 연결될 수 있고 식물에서 발현될 수 있는 것으로 인식된다.
다수의 관심 유전자가 본 발명의 프로모터에 작동 가능하게 연결되어 식물에서 발현될 수 있다. 예를 들어, WUS/WOX 유전자에 곤충 저항성 형질이 쌓일 수 있고, 또한 하나 이상의 추가적 투입 형질(예를 들어, 제초제 저항성, 진균 저항성, 바이러스 저항성, 스트레스 내성, 병해 저항성, 웅성 불임성, 줄기 강도 등) 또는 산출 형질(예를 들어, 증가된 수확량, 변성된 전분, 개선된 오일 프로파일, 균형을 이룬 아미노산, 높은 라이신 또는 메티오닌, 증가된 소화력, 개선된 섬유질, 가뭄 저항성, 영양분 강화 등)이 쌓일 수 있다.
본 발명의 프로모터는 곡물의 품질, 예컨대 오일의 수준(올레산의 함량 증가)과 유형, 라이신과 황의 수준, 셀룰로스의 수준, 및 전분과 단백질 함량을 증가시키는 필수 아미노산의 포화 및 불포화된 품질과 양에 영향을 미치는 작물학적으로 중요한 형질에 작동 가능하게 연결될 수 있다. 본 발명의 프로모터는 콘에서 호르도티오닌 단백질 변형을 제공하는 유전자에 작동 가능하게 연결될 수 있으며, 이러한 유전자는, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,990,389; 5,885,801; 5,885,802 및 5,703,049에 기재되어 있다. 본 발명의 프로모터가 작동 가능하게 연결될 수 있는 유전자의 다른 예는, 1996년 3월20일 출원된 미국 특허 번호 5,850,016에 기재된 대두 2S 알부민에 의해 암호화되는 라이신 및/또는 황이 풍부한 종자 단백질, 및 보리의 키모트립신 억제제(Williamson, et al., (1987) Eur. J. Biochem 165:99-106)이며, 이들의 개시 내용 전체는 본원에 참조로 포함된다.
본 발명의 프로모터는, 근충, 거세미, 유럽옥수수좀 등과 같이 수확량에 큰 방해가 되는 해충에 대한 저항성을 암호화하는 곤충 저항성 유전자에 작동 가능하게 연결될 수 있다. 이러한 유전자는, 예를 들어, 바실러스 투링기엔시스 독성 단백질 유전자(미국 특허 번호 5,366,892; 5,747,450; 5,736,514; 5,723,756; 5,593,881, 및 Geiser, et al., (1986) Gene 48:109)를 포함하며, 이들의 개시 내용 전체는 본원에 참조로 포함된다. 본 발명의 프로모터에 작동 가능하게 연결될 수 있는 병해 저항성 형질을 암호화하는 유전자는, 예를 들어 후모니신을 해독하는 것과 같은 독소제거 유전자(미국 특허 번호 5,792,931); 약독성(avr) 및 병해 저항성(R) 유전자(Jones, et al., (1994) Science 266:789; Martin, et al., (1993) Science 262:1432; 및 Mindrinos, et al., (1994) Cell 78:1089)를 포함하며, 이들 문헌의 전문은 본원에 참조로 포함된다.
본 발명의 프로모터에 작동 가능하게 연결될 수 있는 제초제 저항성 형질은, 아세토락테이트 신타제(ALS)의 작용을 억제하는 작용을 하는 제초제, 특히 설포닐우레아형 제초제에 대한 저항성을 코딩하는 유전자(예를 들어, 이러한 저항성으로 이어지는 돌연변이, 특히 S4 및/또는 Hra 돌연변이를 함유하는 아세토락테이트 신타제(ALS) 유전자), 글루타민 신타제의 작용을 억제하는 작용을 하는 제초제에 대한 저항성을 코딩하는 유전자, 예컨대, 포스피노트리신 또는 바스타(예를 들어, bar 유전자), 글리포세이트에 대한 저항성을 코딩하는 유전자(예를 들어, EPSPS 유전자 및 GAT 유전자; 예를 들어 본원에 전문이 참조로 포함되는 미국 특허 출원 공개 번호 2004/0082770 및 WO 03/092360 참조), 또는 당업계에 알려진 기타 이러한 유전자를 포함한다. bar 유전자는 제초제 바스타에 대한 저항성을 암호화하고, nptII 유전자는 항생제 카나마이신 및 제네티신에 대한 저항성을 암호화하고, ALS 유전자 돌연변이체는 제초제 클로르설푸론에 대한 저항성을 암호화하며, 이들 모두는 본 발명의 프로모터에 작동 가능하게 연결될 수 있다.
글리포세이트 저항성은 본 발명의 프로모터에 작동 가능하게 연결될 수 있는 돌연변이 5-에놀피루블-3-포스피키메이트 신타제(EPSP) 및 aro A 유전자에 의해 부여된다. 예를 들어, 글리포세이트 저항성을 부여할 수 있는 EPSPS 형태의 뉴클레오티드 서열이 개시되어 있는 미국 특허 번호 4,940,835(Shah, et al.) 참조. 미국 특허 번호 5,627,061(Barry, et al.)에도 본 발명의 프로모터에 작동 가능하게 연결될 수 있는 EPSPS 효소를 암호화하는 유전자가 기재되어 있다. 또한, 본원에 전문이 참조로 포함되는 미국 특허 번호 6,248,876 B1; 6,040,497; 5,804,425; 5,633,435; 5,145,783; 4,971,908; 5,312,910; 5,188,642; 4,940,835; 5,866,775; 6,225,114 B1; 6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448; 5,510,471; Re. 36,449; RE 37,287 E 및 5,491,288과 국제 공보 WO 97/04103; WO 97/04114; WO 00/66746; WO 01/66704; WO 00/66747 및 WO 00/66748 참조. 글리포세이트 저항성은 또한, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,776,760 및 5,463,175에 보다 상세히 기재된 바와 같이 글리포세이트 산화환원 효소를 암호화하는, 본 발명의 프로모터에 작동 가능하게 연결될 수 있는 유전자를 발현하는 식물에게 부여된다. 글리포세이트 저항성은 또한, 글리포세이트 N-아세틸트랜스퍼라제를 암호화하는, 본 발명의 프로모터에 작동 가능하게 연결될 수 있는 유전자의 과발현에 의해 식물에게 부여될 수 있다. 또한, 예를 들어 본원에 전문이 참조로 포함되는 미국 특허 출원 일련번호 11/405,845 및 10/427,692 참조.
본 발명의 프로모터에 작동 가능하게 연결되는 불임성 유전자도 DNA 구축물에서 암호화될 수 있고, 물리적 제웅(detasseling)에 대한 대안을 제공한다. 이러한 방식으로 사용되는 유전자의 예는 웅성 조직-선호적 유전자, 및 본원에 전문이 참조로 포함되는 미국 특허 번호 5,583,210에 기재된 QM과 같은 웅성 불임성 표현형을 갖는 유전자를 포함한다. 본 발명의 프로모터에 작동 가능하게 연결될 수 있는 다른 유전자는 키나제 및 웅성 또는 자성 배우체 발육에 유독한 화합물을 암호화하는 것을 포함한다.
예를 들어, 에탄올 생성을 위한 전분을 증가시키거나 단백질의 발현을 제공할 수 있는, 본 발명의 프로모터에 작동 가능하게 연결되는 유전자 또는 유전자에 상업적 형질도 암호화될 수 있다. 형질전환 식물의 다른 중요한 상업적 용도는, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,602,321에 기재된 것과 같은 폴리머 및 바이오플라스틱의 제조이다. 본 발명의 프로모터에 작동 가능하게 연결될 수 있는 β-케토티올라제, PHBase(폴리하이드록시부티레이트 신타제), 및 아세토아세틸-CoA 환원효소와 같은 유전자는(본원에 전문이 참조로 포함되는 문헌[Schubert, et al., (1988) J. Bacteriol. 170:5837-5847] 참조) 폴리하이드록시알카노에이트(PHA)의 발현을 촉진시킨다.
본 발명의 프로모터에 작동 가능하게 연결될 수 있는 기타 적용 가능한 유전자 및 이들의 관련 표현형의 예는 바이러스 외피 단백질 및/또는 RNA를 암호화하는 유전자, 또는 바이러스 저항성을 부여하는 기타 바이러스 또는 식물 유전자; 진균 저항성을 부여하는 유전자; 수확량 향상을 촉진하는 유전자; 및 추위, 가뭄으로 인한 탈수, 열과 염분, 독성 금속 또는 미량 원소 등과 같은 스트레스에 대한 저항성을 제공하는 유전자를 포함한다.
비제한적인 예시로서, 다음은 본 발명의 프로모터 서열에 작동 가능하게 연결될 수 있는 유전자 유형의 다른 예의 목록이다.
1. 곤충이나 병해에 대한 저항성을 부여하고 다음을 암호화하는 이식유전자
(A) 식물 병해 저항성 유전자. 식물 방어는 보통 식물의 병해 저항성 유전자(R)의 산물과 병원체의 상응하는 약독성(Avr) 유전자의 산물 간의 특정 상호작용에 의해 활성화된다. 식물 품종은 클로닝된 저항성 유전자를 사용해 형질전환되어 특정 병원체 균주에 저항성이 있는 식물로 유전자 조작될 수 있다. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Jones, et al., (1994) Science 266:789 (cloning of the tomato Cf-9 gene for resistance to Cladosporium fulvum); Martin, et al., (1993) Science 262:1432 (tomato Pto gene for resistance to Pseudomonas syringae pv. tomato encodes a protein kinase); Mindrinos, et al., (1994) Cell 78:1089 (Arabidopsis RSP2 gene for resistance to Pseudomonas syringae); McDowell and Woffenden, (2003) Trends Biotechnol. 21(4):178-83 및 Toyoda, et al., (2002) Transgenic Res. 11(6):567-82] 참조. 병해 저항성 식물은 야생형 식물에 비해 병원체에 대해 더 저항성이 있는 식물이다.
(B) Bacillus thuringiensis 단백질, 이의 유도체 또는 이를 모델링한 합성 폴리펩티드. 예를 들어, Bt 델타-내독소 유전의 클로닝 및 뉴클레오티드 서열이 개시되어 있는 문헌[Geiser, et al., (1986) Gene 48:109] 참조. 또한 델타-내독소 유전자를 암호화하는 DNA 분자는, 예를 들어 ATCC 수탁번호 40098, 67136, 31995, 및 31998으로 American Type Culture Collection(Rockville, MD)에서 구입할 수 있다. 유전자 조작되는 Bacillus thuringiensis 이식유전자의 다른 예는 다음의 특허 및 특허 출원에 제시되어 있으며, 이 목적을 위해 본원에 참조로 포함된다. 본원에 전문이 참조로 포함되는 미국 특허 번호 5,188,960; 5,689,052; 5,880,275; WO 91/14778; WO 99/31248; WO 01/12731; WO 99/24581; WO 97/40162 및 미국 출원 일련번호 10/032,717; 10/414,637 및 10/606,320.
(C) 에크디스테로이드 및 유충 호르몬과 같은 곤충-특이적 호르몬 또는 페로몬, 이들의 변이체, 이들에 기초한 모방체, 또는 이들의 길항제 또는 작용제. 예를 들어, 유충 호르몬의 불활성화제인 클로닝된 유충 호르몬 에스테라제의 바큘로바이러스 발현에 대한 문헌[Hammock, et al., (1990) Nature 344:458]의 개시 내용 참조(그 전문은 본원에 참조로 포함됨).
(D) 발현시 해당 해충의 생리를 방해하는 곤충-특이적 펩티드. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Regan, (1994) J. Biol. Chem. 269:9(발현 클로닝으로 곤충 이뇨 호르몬 수용체를 코딩하는 DNA를 생성); Pratt, et al., (1989) Biochem. Biophys. Res. Comm.163:1243(Diploptera puntata에서 알로스타틴이 확인됨); Chattopadhyay, et al., (2004) Critical Reviews in Microbiology 30(1):33-54; Zjawiony, (2004) J Nat Prod 67(2):300-310; Carlini and Grossi-de-Sa, (2002) Toxicon 40(11):1515-1539; Ussuf, et al., (2001) Curr Sci. 80(7):847-853 및 Vasconcelos and Oliveira, (2004) Toxicon 44(4):385-403]의 개시 내용 참조. 또한, 곤충-특이적 독소를 암호화하는 유전자가 개시되어 있는 미국 특허 번호 5,266,317(Tomalski, et al.) 참조(그 전문은 본원에 참조로 포함됨).
(E) 모노테르펜, 세스퀴테르펜, 스테로이드, 하이드록삼산, 페닐프로파노이드 유도체, 또는 살충 활성을 갖는 다른 비단백질 분자의 과잉 축적을 초래하는 효소.
(F) 생물학적 활성 분자, 예를 들어 천연 당분해 효소, 단백질분해 효소, 지방분해 효소, 뉴클레아제, 시클라제, 트랜스아미나제, 에스테라제, 가수분해 효소, 포스파타제, 키나제, 포스포릴라제, 중합효소, 엘라스타제, 키티나제, 및 글루카나제의 변형(번역후 변형 포함)에 관여하는 효소(천연이든 합성이든 무관). 칼라제 유전자의 뉴클레오티드 서열이 개시되어 있는 PCT 출원 번호 WO 93/02197(Scott, et al.) 참조(그 전문은 본원에 참조로 포함됨). 키티나제-암호화 서열을 함유하는 DNA 분자는, 예를 들어 ATCC 수탁번호 39637 및 67152로부터 얻을 수 있다. 또한, 담배 구충 키티나제를 암호화하는 cDNA의 뉴클레오티드 서열이 교시되어 있는 문헌[Kramer, et al., (1993) Insect Biochem. Molec. Biol. 23:691], 파슬리 ubi4-2 폴리유비퀴틴 유전자의 뉴클레오티드 서열을 제공하는 문헌[Kawalleck, et al., (1993) Plant Molec. Biol. 21:673], 미국 특허 출원 일련번호 10/389,432, 10/692,367, 및 미국 특허 번호 6,563,020 참조(이들의 전문은 본원에 참조로 포함됨).
(G) 신호 변환을 자극하는 분자. 예를 들어, 녹두 칼모듈린 cDNA 클론의 뉴클레오티드 서열에 대한 문헌[Botella, et al., (1994) Plant Molec. Biol. 24:757]의 개시 내용, 및 옥수수 칼모듈린 cDNA 클론에 대한 뉴클레오티드 서열을 제공하는 문헌[Griess, et al., (1994) Plant Physiol.104:1467] 참조(이들의 전문은 본원에 참조로 포함됨).
(H) 소수성 모멘트 펩티드. 본원에 전문이 참조로 포함되는 PCT 출원 번호 WO 95/16776 및 미국 특허 번호 5,580,852(진균성 식물 병원체를 억제하는 타키플레신의 펩티드 유도체에 대한 개시 내용) 및 PCT 출원 번호 WO 95/18855 및 미국 특허 번호 5,607,914(병해 저항성을 부여하는 합성 항균 펩티드를 교시).
(I) 멤브레인 투과효소, 채널 형성제, 또는 채널 차단제. 예를 들어, Pseudomonas solanacearum에 저항성이 있는 유전자이식 담배 식물을 만드는 세크로핀-베타 용해성 펩티드 유사체의 이종 발현에 대한 문헌[Jaynes, et al., (1993) Plant Sci. 89:43]의 개시 내용 참조(그 전문은 본원에 참조로 포함됨).
(J) 바이러스-침습성 단백질 또는 이로부터 유도된 복합 독소. 예를 들어, 형질전환 식물 세포 내 바이러스 외피 단백질의 축적은 바이러스 감염에 대한 저항성, 및/또는 외피 단백질 유전자가 유도되는 바이러스 및 관련 바이러스에 의한 병해 발생에 대한 저항성을 부여한다. 본원에 전문이 참조로 포함되는 문헌[Beachy, et al., (1990) Ann. Rev. Phytopathol. 28:451] 참조. 외피 단백질-매개 저항성은 알팔파 모자이크 바이러스, 오이 모자이크 바이러스, 담배 줄무늬 바이러스, 감자 바이러스 X, 감자 바이러스 Y, 담배 에칭 바이러스(tobacco etch virus), 담배 래틀 바이러스(tobacco rattle virus), 및 담배 모자이크 바이러스에 대해 형질전환 식물에 부여된 바 있다(상기 문헌).
(K) 곤충-특이적 항체 또는 이로부터 유도된 면역독소. 따라서, 곤충 소화관에서의 중요한 대사 기능에 대해 표적화된 항체는 영향을 받은 효소를 불활성화시켜 곤충을 죽인다. 본원에 전문이 참조로 포함되는 문헌[Taylor, et al., 요약 #497, SEVENTH INT'L SYMPOSIUM ON MOLECULAR PLANT-MICROBE INTERACTIONS (Edinburgh, Scotland, 1994)] (단일쇄 항체 단편의 생성을 통한 유전자이식 담배에서의 효소 불활성화) 참조.
(L) 바이러스-특이적 항체. 예를 들어, 재조합 항체 유전자를 발현하는 유전자이식 식물이 바이러스 공격으로부터 보호됨을 나타내는 문헌[Tavladoraki, et al., (1993) Nature 366:469] 참조(그 전문은 본원에 참조로 포함됨).
(M) 병원체 또는 기생충에 의해 자연적으로 생성된 발육 억제 단백질. 따라서, 진균성 엔도 알파-1,4-D-폴리갈락투로나제는 식물 세포벽 호모-알파-1,4-D-갈락투로나제를 가용화함으로써 진균 군집화 및 식물 영양분 방출을 용이하게 한다. 본원에 전문이 참조로 포함되는 문헌[Lamb, et al., (1992) Bio/Technology 10:1436] 참조. 콩 엔도폴리갈락투로나제-억제 단백질을 암호화하는 유전자의 클로닝 및 특성화는, 본원에 전문이 참조로 포함되는 문헌[Toubart, et al., (1992) Plant J. 2:367]에 기재되어 있다.
(N) 식물에 의해 자연적으로 생성된 발육 억제 단백질. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Logemann, et al., (1992) Bio/Technology 10:305]은 보리 리보좀-불활성화 유전자를 발현하는 유전자이식 식물이 진균성 병해에 대해 증가된 저항성을 가짐을 보여주었다.
(O) 전신 획득 저항성(SAR) 반응 및/또는 병인 관련 유전자에 관여하는 유전자. 본원에 전문이 참조로 포함되는 문헌[Briggs, (1995) Current Biology 5(2):128-131, Pieterse and Van Loon, (2004) Curr. Opin. Plant Bio. 7(4):456-64 및 Somssich, (2003) Cell 113(7):815-6].
(P) 항진균성 유전자(Cornelissen and Melchers, (1993) Pl. Physiol. 101:709-712 및 Parijs, et al., (1991) Planta 183:258-264 및 Bushnell, et al., (1998) Can. J. of Plant Path. 20(2):137-149). 또한, 본원에 전문이 참조로 포함되는 미국 특허 출원 번호 09/950,933 참조.
(Q) 후모니신, 뷰베리신, 모닐리포르민, 및 제랄레논 등에 대한 독소제거 유전자, 및 이들의 구조적으로 관련된 유도체. 예를 들어, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,792,931 참조.
(R) 시스타틴 및 시스타틴 프로테이나제 억제제. 본원에 전문이 참조로 포함되는 미국 출원 일련번호 10/947,979 참조.
(S) 디펜신 유전자. 본원에 전문이 참조로 포함되는 WO03/000863 및 미국 출원 일련번호 10/178,213 참조.
(T) 선충류에 대한 저항성을 부여하는 유전자. 본원에 전문이 참조로 포함되는 WO 03/033651 및 문헌[Urwin, et. al., (1998) Planta 204:472-479, Williamson (1999) Curr Opin Plant Bio. 2(4):327-31] 참조.
(U) 진균 Colletotrichum graminiola에 의해 발생하는 탄저병 줄기 썩음에 대한 저항성을 부여하는 rcg1과 같은 유전자. 본원에 전문이 참조로 포함되는 문헌[Jung, et al., Generation-means analysis and quantitative trait locus mapping of Anthracnose Stalk Rot genes in Maize, Theor. Appl. Genet. (1994) 89:413-418], 뿐만 아니라 미국 가출원 번호 60/675,664 참조.
2. 제초제에 대한 저항성을 부여하는 이식유전자, 예를 들어,
(A) 성장점 또는 분열조직을 억제하는 제초제, 예컨대 이미다졸리논 또는 설포닐우레아. 이 범주 내의 예시적 유전자는, 예를 들어 문헌[Lee, et al., (1988) EMBO J. 7:1241] 및 문헌[Miki, et al., (1990) Theor. Appl. Genet. 80:449]에 각각 기재된 바와 같이 돌연변이 ALS 및 AHAS 효소를 코딩한다. 또한, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,605,011; 5,013,659; 5,141,870; 5,767,361; 5,731,180; 5,304,732; 4,761,373; 5,331,107; 5,928,937 및 5,378,824와 국제 공보 WO 96/33270 참조.
(B) 글리포세이트(돌연변이 5-에놀피루블-3-포스피키메이트 신타제(EPSP) 및 aroA 유전자에 의해 각각 부여되는 저항성), 기타 포스포노 화합물, 예컨대 글루포시네이트(포스피노트리신 아세틸 트랜스퍼라제(PAT) 및 Streptomyces hygroscopicus 포스피노트리신 아세틸 트랜스퍼라제 (bar) 유전자), 및 피리딘옥시 또는 페녹시 프로프리온산 및 시클로헥손(ACCase 억제제-암호화 유전자). 예를 들어, 글리포세이트 저항성을 부여할 수 있는 EPSPS 형태의 뉴클레오티드 서열이 개시되어 있는 미국 특허 번호 4,940,835(Shah, et al.) 참조. 미국 특허 번호 5,627,061(Barry, et al.)에도 EPSPS 효소를 암호화하는 유전자가 기재되어 있다. 또한, 본원에 전문이 참조로 포함되는 미국 특허 번호 6,566,587; 6,338,961; 6,248,876 B1; 6,040,497; 5,804,425; 5,633,435; 5,145,783; 4,971,908; 5,312,910; 5,188,642; 4,940,835; 5,866,775; 6,225,114 B1; 6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448; 5,510,471; Re. 36,449; RE 37,287 E 및 5,491,288과 국제 공보 EP1173580; WO 01/66704; EP1173581 및 EP1173582 참조. 글리포세이트 저항성은 또한, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,776,760 및 5,463,175에 보다 상세히 기재된 바와 같이 글리포세이트 산화환원 효소를 암호화하는 유전자를 발현하는 식물에게 부여된다. 또한, 글리포세이트 저항성은 글리포세이트 N-아세틸트랜스퍼라제를 암호화하는 유전자의 과발현에 의해 식물에게 부여될 수 있다. 예를 들어, 본원에 전문이 참조로 포함되는 미국 특허 출원 일련번호 11/405,845 및 10/427,692와 PCT 출원 번호 US01/46227 참조. 돌연변이 aroA 유전자를 암호화하는 DNA 분자는 ATCC 수탁번호 39256으로 얻을 수 있으며, 이 돌연변이 유전자의 뉴클레오티드 서열은 본원에 전문이 참조로 포함되는 미국 특허 번호 4,769,061(Comai)에 개시되어 있다. 본원에 전문이 참조로 포함되는 EP 특허 출원 번호 0 333 033(Kumada, et al.), 및 미국 특허 번호 4,975,374(Goodman, et al.)에는 L-포스피노트리신과 같은 제초제에 대한 저항성을 부여하는 글루타민 신테타제 유전자의 뉴클레오티드 서열이 개시되어 있다. 포스피노트리신-아세틸-트랜스퍼라제 유전자의 뉴클레오티드 서열은, 포스피노트리신 아세틸 트랜스퍼라제 활성을 코딩하는 키메라 bar 유전자를 발현하는 유전자이식 식물의 제조를 설명하는 EP 특허 번호 0 242 246 및 0 242 236(Leemans, et al.), 문헌[De Greef, et al., (1989) Bio/Technology 7:61]에 제공되어 있으며, 이들 문헌의 전문은 본원에 참조로 포함된다. 또한, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,969,213; 5,489,520; 5,550,318; 5,874,265; 5,919,675; 5,561,236; 5,648,477; 5,646,024; 6,177,616 B1 및 5,879,903 참조. 세톡시딤 및 할록시포프와 같은 페녹시 프로프리온산 및 시클로헥손에 대한 저항성을 부여하는 예시적 유전자는 본원에 전문이 참조로 포함되는 문헌[Marshall, et al., (1992) Theor. Appl. Genet. 83:435]에 기재된 Acc1-S1, Acc1-S2 및 Acc1-S3 유전자이다.
(C) 광합성을 억제하는 제초제, 예컨대 트리아진(psbA 및 gs+ 유전자) 및 벤조니트릴(니트릴라제 유전자). 본원에 전문이 참조로 포함되는 문헌[Przibilla, et al., (1991) Plant Cell 3:169]에는 돌연변이 psbA 유전자를 암호화하는 플라스미드를 이용한 Chlamydomonas의 형질전환이 기술되어 있다. 니트릴라제 유전자에 대한 뉴클레오티드 서열은 본원에 전문이 참조로 포함되는 미국 특허 번호 4,810,648(Stalker)에 기재되어 있으며, 이들 유전자를 함유하는 DNA 분자는 ATCC 수탁번호 53435, 67441, 및 67442로 입수 가능하다. 글루타티온 S-트랜스퍼라제를 코딩하는 DNA의 클로닝 및 발현은 본원에 전문이 참조로 포함되는 문헌[Hayes, et al., (1992) Biochem. J. 285:173]에 기술되어 있다.
(D) 이 효소를 발현하는 식물을 다양한 유형의 제초제에 저항성이 있도록 하는 것으로 밝혀진 아세토하이드록시산 신타제는 다양한 식물에 도입된 바 있다(예를 들어, 본원에 전문이 참조로 포함되는 문헌[Hattori, et al., (1995) Mol Gen Genet 246:419] 참조). 제초제에 대한 저항성을 부여하는 기타 유전자는 랫트 시토크롬 P4507A1과 효모 NADPH-시토크롬 P450 산화환원 효소의 키메라 단백질을 암호화하는 유전자(Shiota, et al., (1994) Plant Physiol. 106(1):17-23), 글루타티온 환원효소와 수퍼옥사이드 디스뮤타제에 대한 유전자(Aono, et al., (1995) Plant Cell Physiol 36:1687), 및 다양한 포스포트랜스퍼라제에 대한 유전자(Datta, et al., (1992) Plant Mol Biol 20:619)를 포함하며, 이들 문헌의 전문은 본원에 참조로 포함된다.
(E) 프로토포르피리노겐 산화효소(프로톡스)는 모든 식물 생존에 필요한 엽록소를 생성하는 데 필요하다. 프로톡스 효소는 다양한 제초제 화합물에 대한 표적 역할을 한다. 이러한 제초제는 또한, 존재하는 모든 상이한 종의 식물의 성장을 억제하여 이들을 완전히 파괴시킨다. 이러한 제초제에 저항성이 있는 변경된 프로톡스 활성을 갖는 식물의 개발은 본원에 전문이 참조로 포함되는 미국 특허 번호 6,288,306 B1; 6,282,837 B1 및 5,767,373; 국제 공개 번호 WO 01/12825에 기술되어 있다.
3. 하기와 같은 변경된 곡물 특성을 부여하거나 이에 기여하는 이식유전자,
(A) 예를 들어, 하기에 의해 변경된 지방산
(1) 식물의 스테아르산 함량을 증가시키기 위한 스테아로일-ACP 불포화효소의 하향 조절. 본원에 전문이 참조로 포함되는 문헌[Knultzon, et al., (1992) Proc. Natl. Acad. Sci. USA 89:2624] 및 WO99/64579(Genes for Desaturases to Alter Lipid Profiles in Corn) 참조,
(2) FAD-2 유전자 변형을 통한 올레산의 증가 및/또는 FAD-3 유전자 변형을 통한 리놀렌산의 감소(본원에 전문이 참조로 포함되는 미국 특허 번호 6,063,947; 6,323,392; 6,372,965, 및 WO 93/11245 참조),
(3) 본원에 전문이 참조로 포함되는 WO 01/12800에서와 같은, 공액 리놀렌산 또는 리놀레산 함량의 변경,
(4) LEC1, AGP, Dek1, Superal1, mi1ps, 다양한 lpa 유전자(예컨대 lpa1, lpa3, hpt, 또는 hggt)의 변경. 예를 들어, 본원에 전문이 참조로 포함되는 WO 02/42424, WO 98/22604, WO 03/011015, 미국 특허 번호 6,423,886, 미국 특허 번호 6,197,561, 미국 특허 번호 6,825,397, 미국 특허 출원 공개 번호 2003/0079247, 2003/0204870, WO02/057439, WO03/011015 및 문헌[Rivera-Madrid, et. al., (1995) Proc. Natl. Acad. Sci. 92:5620-5624] 참조.
(B) 예를 들어, 하기에 의해 변경된 인 함량
(1) 피타제-암호화 유전자의 도입은 형질전환 식물에 더 많은 유리 인산염을 첨가하여 피틴산염의 분해를 향상시킬 것이다. 예를 들어, Aspergillus niger 피타제 유전자의 뉴클레오티드 서열의 개시 내용에 대해 문헌[Van Hartingsveldt, et al., (1993) Gene 127:87] 참조(그 전문은 본원에 참조로 포함됨).
(2) 피틴산염 함량을 감소시키는 유전자의 상향 조절. 옥수수에서, 이는 예를 들어 문헌[Raboy, et al., (1990) Maydica 35:383]에서와 같이, 낮은 수준의 피틴산을 특징으로 하는 옥수수 돌연변이체에서 확인되는 LPA 대립유전자와 같은 하나 이상의 대립유전자와 관련된 DNA를 클로닝한 후 재도입함으로써, 및/또는 본원에 전문이 참조로 포함되는 WO 02/059324, 미국 특허 출원 공개 번호 2003/0009011, WO 03/027243, 미국 특허 출원 공개 번호 2003/0079247, WO 99/05298, 미국 특허 번호 6,197,561, 미국 특허 번호 6,291,224, 미국 특허 번호 6,391,348, WO2002/059324, 미국 특허 출원 공개 번호 2003/0079247, WO98/45448, WO99/55882, WO01/04147에서와 같이 이노시톨 키나제 활성을 변경함으로써 달성될 수 있다.
(C) 예를 들어, 전분의 분지화 패턴에 영향을 미치는 효소에 대한 유전자 또는 NTR 및/또는 TRX와 같은 티오레독신을 변경하는 유전자(전문이 참조로 포함되는 미국 특허 번호 6,531,648 참조), 및/또는 cs27 또는 TUSC27 또는 en27과 같은 감마 제인 녹아웃 또는 돌연변이체(전문이 참조로 포함되는 미국 특허 번호 6,858,778 및 미국 특허 출원 공개 번호 2005/0160488 및 2005/0204418 참조)를 변경함으로써 변경된 탄수화물. 본원에 전문이 참조로 포함되는 문헌[Shiroza, et al., (1988) J. Bacteriol. 170:810(스트렙토코구스 뮤턴트 프룩토실트랜스퍼라제 유전자의 뉴클레오티드 서열), Steinmetz, et al., (1985) Mol. Gen. Genet. 200:220(바실러스 서브틸리스 레반수크라제 유전자의 뉴클레오티드 서열), Pen, et al., (1992) Bio/Technology 10:292(바실러스 리케니포미스 알파-아밀라제를 발현하는 유전자이식 식물의 제조), Elliot, et al., (1993) Plant Molec. Biol. 21:515(토마토 인버타제 유전자의 뉴클레오티드 서열), Søgaard, et al., (1993) J. Biol. Chem. 268:22480(보리 알파-아밀라제 유전자의 부위-특이적 돌연변이 유도) 및 Fisher, et al., (1993) Plant Physiol. 102:1045(옥수수 배젖 전분 분지화 효소 II)], WO 99/10498(UDP-D-자일로스 4-에피머라제, Fragile 1 및 2, Ref1, HCHL, C4H의 변형을 통한 개선된 소화성 및/또는 전분 추출), 미국 특허 번호 6,232,529(method of producing high oil seed by modification of starch levels (AGP)) 참조. 상기 언급된 지방산 변형 유전자는 또한, 전분과 오일의 경로의 상호관계를 통해 전분 함량 및/또는 조성에 영향을 주기 위해 사용될 수 있다.
(D) 토코페롤 또는 토코트리에놀의 변경과 같은 변경된 항산화제 함량 또는 조성. 예를 들어, 피틀 프레닐 트랜스퍼라제(ppt)의 변경을 통한 항산화제 수준의 조작을 수반하는 미국 특허 번호 6,787,683, 미국 특허 출원 공개 번호 2004/0034886, 및 호모겐티스산 게라닐 게라닐 트랜스퍼라제(hggt)의 변경을 통한 WO 00/68393 참조(이들의 전문은 본원에 참조로 포함됨).
(E) 변경된 필수 종자 아미노산. 예를 들어, 본원에 전문이 참조로 포함되는 미국 특허 번호 6,127,600(method of increasing accumulation of essential amino acids in seeds), 미국 특허 번호 6,080,913(binary methods of increasing accumulation of essential amino acids in seeds), 미국 특허 번호 5,990,389(높은 라이신), WO99/40209(alteration of amino acid compositions in seeds), WO99/29882(methods for altering amino acid content of proteins), 미국 특허 번호 5,850,016(alteration of amino acid compositions in seeds), WO98/20133(proteins with enhanced levels of essential amino acids), 미국 특허 번호 5,885,802(높은 메티오닌), 미국 특허 번호 5,885,801(높은 트레오닌), 미국 특허 번호 6,664,445(plant amino acid biosynthetic enzymes), 미국 특허 번호 6,459,019(증가된 라이신 및 트레오닌), 미국 특허 번호 6,441,274(plant tryptophan synthase beta subunit), 미국 특허 번호 6,346,403(methionine metabolic enzymes), 미국 특허 번호 5,939,599(높은 황), 미국 특허 번호 5,912,414(증가된 메티오닌), WO98/56935(plant amino acid biosynthetic enzymes), WO98/45458(engineered seed protein having higher percentage of essential amino acids), WO98/42831(증가된 라이신), 미국 특허 번호 5,633,436(황 아미노산 함량 증가), 미국 특허 번호 5,559,223(synthetic storage proteins with defined structure containing programmable levels of essential amino acids for improvement of the nutritional value of plants), WO96/01905(증가된 트레오닌), WO95/15392(증가된 라이신), 미국 특허 출원 공개 번호 2003/0163838, 미국 특허 출원 공개 번호 2003/0150014, 미국 특허 출원 공개 번호 2004/0068767, 미국 특허 번호 6,803,498, WO01/79516, 및 WO00/09706(Ces A: 셀룰로스 신타제), 미국 특허 번호 6,194,638(헤미셀룰로스), 미국 특허 번호 6,399,859 및 미국 특허 출원 공개 번호 2004/0025203(UDPGdH), 미국 특허 번호 6,194,638(RGP) 참조.
4. 부위-특이적 DNA 통합을 위한 부위를 생성하는 유전자
이는 FLP/FRT 시스템에서 사용될 수 있는 FRT 부위 및/또는 Cre/Loxp 시스템에서 사용될 수 있는 Lox 부위의 도입을 포함한다. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[Lyznik, et al., (2003) Plant Cell Rep 21:925-932] 및 WO 99/25821 참조. 사용될 수 있는 다른 시스템은 파지 Mu의 Gin 재조합효소(Maeser, et al., 1991; Vicki Chandler, The Maize Handbook ch. 118 (Springer-Verlag 1994), 대장균의 Pin 재조합효소(Enomoto, et al., 1983), 및 pSR1 플라스미드의 R/RS 시스템(Araki, et al., 1992)을 포함하며, 이들 문헌의 전문은 본원에 참조로 포함된다.
5. 무생물 스트레스 저항성(개화, 이삭 및 종자 발육, 질소 이용 효율, 변경된 질소 반응성, 가뭄 저항성 또는 내성, 추위 저항성 또는 내성, 및 염 저항성 또는 내성을 포함하나, 이에 한정되지 않음) 및 스트레스하에서의 증가된 수확량에 영향을 미치는 유전자. 예를 들어, 말레이트의 변경을 통해 물 이용 효율이 변경됨을 보여주는 WO 00/73475; 식물에 대한 결빙, 높은 염분, 및 가뭄의 부정적인 영향을 완화시킬 뿐만 아니라 식물 표현형에 대한 기타 긍정적인 효과를 부여하는 데 효과적인 CBF 유전자 및 전사 인자를 포함한 유전자가 기재되어 있는 미국 특허 번호 5,892,009, 미국 특허 번호 5,965,705, 미국 특허 번호 5,929,305, 미국 특허 번호 5,891,859, 미국 특허 번호 6,417,428, 미국 특허 번호 6,664,446, 미국 특허 번호 6,706,866, 미국 특허 번호 6,717,034, WO2000060089, WO2001026459, WO2001035725, WO2001034726, WO2001035727, WO2001036444, WO2001036597, WO2001036598, WO2002015675, WO2002017430, WO2002077185, WO2002079403, WO2003013227, WO2003013228, WO2003014327, WO2004031349, WO2004076638, WO9809521, 및 WO9938977; 식물에서 아브시스산이 변경되어 수확량 증가 및/또는 무생물 스트레스에 대한 내성 증가와 같은 식물 표현형 개선으로 이어짐을 보여주는 미국 특허 출원 공개 번호 2004/0148654 및 WO01/36596; 사이토키닌 발현이 변형되어 가뭄 내성과 같은 스트레스 내성이 증가된 식물, 및/또는 수확량 증가로 이어짐을 보여주는 WO2000/006341, WO04/090143, 미국 특허 출원 일련번호 10/817483 및 미국 특허 번호 6,992,237 참조(이들의 전문은 본원에 참조로 포함됨). 또한, 본원에 전문이 참조로 포함되는 WO0202776, WO2003052063, JP2002281975, 미국 특허 번호 6,084,153, WO0164898, 미국 특허 번호 6,177,275 및 미국 특허 번호 6,107,547(질소 이용률 향상 및 변경된 질소 반응성) 참조. 에틸렌 변경에 대해서는, 본원에 전문이 참조로 포함되는 미국 특허 출원 공개 번호 2004/0128719, 미국 특허 출원 공개 번호 2003/0166197, 및 WO200032761 참조. 무생물 스트레스의 식물 전사 인자 또는 전사 조절 인자에 대해서는, 본원에 전문이 참조로 포함되는 미국 특허 출원 공개 번호 2004/0098764 또는 미국 특허 출원 공개 번호 2004/0078852 참조.
6. 식물 성장에 영향을 미치고 수확량, 개화, 식물 성장, 및/또는 식물 구조와 같은 작물학적 형질에 영향을 미치는 기타 유전자 및 전사 인자가 식물에 도입 또는 혼입될 수 있다(예를 들어, 본원에 전문이 참조로 포함되는 WO97/49811(LHY), WO98/56918(ESD4), WO97/10339, 및 미국 특허 번호 6,573,430(TFL), 미국 특허 번호 6,713,663(FT), WO96/14414(CON), WO96/38560, WO01/21822(VRN1), WO00/44918(VRN2), WO99/49064(GI), WO00/46358(FRI), WO97/29123, 미국 특허 번호 6,794,560, 미국 특허 번호 6,307,126(GAI), WO99/09174 (D8 및 Rht), WO2004076638 및 WO2004031349(전사 인자) 참조).
본원에 개시된 프로모터 서열에 작동 가능하게 연결되는 이종 뉴클레오티드 서열 및 이와 관련된 생물학적 활성 단편 또는 변이체는 표적 유전자에 대한 안티센스 서열일 수 있다. 용어 "안티센스 DNA 뉴클레오티드 서열"은 뉴클레오티드 서열의 5'에서 3'으로의 정상적인 방향에 대해 반대 방향인 서열을 의미하는 것이다. 식물 세포 내로 전달되는 경우, 안티센스 DNA 서열의 발현은 표적 유전자에 대한 DNA 뉴클레오티드 서열의 정상적인 발현을 막는다. 안티센스 뉴클레오티드 서열은 표적 유전자에 대한 DNA 뉴클레오티드 서열의 전사에 의해 생성되는 내인성 메신저 RNA(mRNA)에 상보적이고 혼성화될 수 있는 RNA 전사체를 암호화한다. 이 경우, 표적 유전자에 의해 암호화되는 천연 단백질의 생성은 원하는 표현형 반응을 달성하도록 억제된다. 서열이 해당 mRNA에 혼성화되고 그 발현을 방해하는 한, 안티센스 서열의 변형은 이루어질 수 있다. 이러한 방식으로, 해당 안티센스 서열과 70%, 80%, 85%의 서열 동일성을 갖는 안티센스 구축물이 사용될 수 있다. 또한, 안티센스 뉴클레오티드의 일부는 표적 유전자의 발현을 방해하는 데 사용될 수 있다. 일반적으로, 적어도 50개의 뉴클레오티드, 100개의 뉴클레오티드, 200개 이상의 뉴클레오티드의 서열이 사용될 수 있다. 따라서, 본원에 개시된 프로모터 서열은 안티센스 DNA 서열에 작동 가능하게 연결되어 식물에서 천연 단백질의 발현을 감소시키거나 억제할 수 있다.
"RNAi"는 유전자의 발현을 감소시키기 위한 일련의 관련 기술을 의미한다(예를 들어, 본원에 전문이 참조로 포함되는 미국 특허 번호 6,506,559 참조). 다른 명칭으로 언급되는 더 오래된 기술은 현재 동일한 메커니즘을 이용할 것으로 생각되지만, 문헌에서는 다른 명칭으로 사용된다. 이들은 표적 단백질의 발현을 억제할 수 있는 안티센스 RNA 전사체의 생성인 "안티센스 억제", 및 동일하거나 실질적으로 유사한 외래성 또는 내인성 유전자의 발현을 억제할 수 있는 센스 RNA 전사체의 생성을 의미하는 "보조 억제" 또는 "센스 억제"를 포함한다(본원에 전문이 참조로 포함되는 미국 특허 번호 5,231,020). 이러한 기술은 침묵될 표적 유전자에 상보적인 하나의 가닥을 갖는 이중가닥 RNA의 축적을 생기게 하는 구축물의 사용을 필요로 한다. 본 발명의 프로모터 서열은, 마이크로RNA 및 siRNA를 포함하는 RNA 간섭을 생기게 할 구축물의 발현을 유도하기 위해 사용될 수 있다.
본원에 사용된 용어 "프로모터" 또는 "전사 개시 영역"은 특정 코딩 서열에 대해 적절한 전사 개시 부위에서 RNA 합성을 개시하도록 RNA 중합효소 II에 명령할 수 있는 TATA 박스 또는 DNA 서열을 대개 포함하는 DNA의 조절 영역을 의미한다. 프로모터는 RNA 합성을 개시하도록 RNA 중합효소 II에 명령할 수 있는 TATA 박스 또는 DNA 서열의 상류 또는 5'에 일반적으로 위치하는, 상류 프로모터 요소로 지칭되는 다른 인식 서열을 추가로 포함할 수 있고, 이들은 전사 개시 속도에 영향을 미친다. 본원에 개시된 프로모터 영역에 대한 뉴클레오티드 서열을 확인한 경우, 본원에서 확인된 특정 프로모터 영역으로부터 상류의 5' 미번역 영역에서 추가의 프로모터를 단리하고 동정하는 것은 최신 기술에 속하는 것으로 인식된다. 또한, 키메라 프로모터가 제공될 수 있다. 이러한 키메라는 이종 전사 조절 영역의 단편 및/또는 변이체에 융합된 프로모터 서열의 일부를 포함한다. 따라서, 본원에 개시된 프로모터 영역은 코딩 서열, 인핸서 등의 조직 및 일시적 발현을 담당하는 것들과 같은 상류 프로모터를 포함할 수 있다.
본원에 사용된 용어 "조절 요소"는 또한, 항상 그런 것은 아니지만 일반적으로 구조 유전자의 코딩 서열의 상류(5')에 있는 DNA의 서열을 의미하며, 이는 RNA 중합효소 및/또는 특정 부위에서 전사를 개시하는 데 필요한 다른 인자에 대한 인식을 제공함으로써 코딩 영역의 발현을 제어하는 서열을 포함한다. 특정 부위에서의 개시를 보장하도록 RNA 중합효소 또는 다른 전사 인자에 대한 인식을 제공하는 조절 요소의 예는 프로모터 요소이다. 프로모터 요소는 전사의 개시를 담당하는 코어 프로모터 요소뿐만 아니라, 유전자 발현을 변형시키는 다른 조절 요소를 포함한다. 코딩 영역 서열의 인트론 또는 3' 내에 위치한 뉴클레오티드 서열은 또한 관심 코딩 영역의 발현 조절에 기여할 수 있음을 이해해야 한다. 적합한 인트론의 예는 옥수수 IVS6 인트론, 또는 옥수수 액틴 인트론을 포함하나, 이에 한정되지 않는다. 조절 요소는 또한, 전사 개시 부위의 하류(3') 및/또는 전사된 영역 내에 위치한 요소를 포함할 수 있다. 본 발명과 관련하여, 전사후 조절 요소는 전사 개시 후에 활성인 요소, 예를 들어 번역 및 전사 인핸서, 번역 및 전사 리프레서, 및 mRNA 안정성 결정 인자를 포함할 수 있다.
본 발명의 프로모터 또는 이들의 변이체 또는 단편은 이종 조절 요소 또는 프로모터와 작동적으로 결합되어 이종 조절 요소의 활성을 조절할 수 있다. 이러한 조절은 이종 조절 요소의 전사 활성의 강화 또는 억제, 및/또는 전사후 이벤트의 조절을 포함한다. 예를 들어, 본 발명의 하나 이상의 프로모터 또는 이들의 단편은 항시성, 유도성, 또는 조직-특이적 프로모터 또는 이들의 단편과 작동적으로 결합되어 식물 세포의 원하는 조직 내에서 이러한 프로모터의 활성을 조절할 수 있다.
본 발명의 프로모터 서열 또는 이들의 변이체 또는 단편은, 형태형성 유전자 및/또는 이종의 관심 뉴클레오티드 서열에 작동 가능하게 연결될 경우, 식물의 L1 조직에서 형태형성 유전자 및/또는 이종 뉴클레오티드 서열의 안정적 또는 일시적 발현을 유도할 수 있다.
본 명세서 전체에 걸쳐 사용된 "이종 뉴클레오티드 서열", "이종의 관심 폴리뉴클레오티드", 또는 "이종 폴리뉴클레오티드"는 본 발명의 프로모터 서열에 작동 가능하게 연결되거나 이와 자연적으로 발생하지 않는 서열이다. 이러한 뉴클레오티드 서열은 프로모터 서열에 이종성이지만, 식물 숙주에 상동성이거나 고유하거나 이종성이거나 외래성일 수 있다. 마찬가지로, 프로모터 서열은 식물 숙주 및/또는 관심 폴리뉴클레오티드에 상동성이거나 고유하거나 이종성이거나 외래성일 수 있다.
본 발명의 단리된 프로모터 서열은 이종 뉴클레오티드 서열의 다양한 발현 수준을 제공하도록 변형될 수 있다. 따라서, 전체 프로모터 영역보다 적게 이용될 수 있고, 관심 뉴클레오티드 서열의 발현을 유도하는 능력이 유지된다. mRNA의 발현 수준은 프로모터 서열의 일부의 결실에 따라 상이한 방식으로 변경될 수 있는 것으로 인식된다. mRNA 발현 수준은 감소될 수 있거나, 대안적으로, 예를 들어 절단 프로세스 중에 제거되는 (리프레서에 대해) 부정적인 조절 요소가 존재하는 경우, 프로모터 결손으로 인해 발현이 증가될 수 있다. 일반적으로, 단리된 프로모터 서열의 적어도 약 20개의 뉴클레오티드가 뉴클레오티드 서열의 발현을 유도하는 데 사용될 것이다.
전사 수준을 높이기 위해 본 발명의 프로모터 영역과 함께 인핸서가 이용될 수 있는 것으로 인식된다. 인핸서는 프로모터 영역의 발현을 증가시키는 작용을 하는 뉴클레오티드 서열이다. 인핸서는 당업계에 알려져 있으며, SV40 인핸서 영역, 35S 인핸서 요소 등을 포함한다. 일부 인핸서는, 예를 들어 인핸서 없이 동일한 프로모터가 하나의 특정 조직 또는 소수의 특정 조직에서만 발현되는 경우 프로모터를 항시적으로 발현시킴으로써, 정상적인 프로모터 발현 패턴을 변경하는 것으로 알려져 있다.
본 발명의 단리된 프로모터 서열의 변형은 이종 뉴클레오티드 서열의 다양한 발현을 제공할 수 있다. 따라서, 이들은 약한 프로모터 또는 강한 프로모터로 변형될 수 있다. 일반적으로, "약한 프로모터"는 낮은 수준의 코딩 서열 발현을 유도하는 프로모터를 의미한다. "낮은 수준"의 발현은 약 1/10,000 전사 내지 약 1/100,000 전사 내지 약 1/500,000 전사 수준의 발현을 의미하는 것이다. 반대로, 강한 프로모터는 높은 수준, 또는 약 1/10 전사 내지 약 1/100 전사 내지 약 1/1,000 전사 수준의 코딩 서열 발현을 유도한다.
본 발명의 프로모터는 천연 코딩 서열과 함께 사용되어 발현을 증가 또는 감소시킴으로써 형질전환 식물의 표현형을 변화시킬 수 있는 것으로 인식된다. 본원에 개시된 뉴클레오티드 서열(표 1 참조), 뿐만 아니라 이들의 변이체 및 단편은 임의의 식물의 유전자 조작에 유용하다. 조절 서열은, 원하는 표현형 반응을 달성하도록 발현이 제어되어야 하는 이종 뉴클레오티드 서열과 작동 가능하게 연결되는 경우 이러한 양태에서 유용하다. 용어 "작동 가능하게 연결"은 이종 뉴클레오티드 서열의 전사 또는 번역이 프로모터 서열의 영향을 받고 있음을 의미한다. 이러한 방식으로, 본 발명의 프로모터에 대한 뉴클레오티드 서열은 관심 식물에서의 발현을 위해, 보다 구체적으로는 식물의 생식 조직에서의 발현을 위해 이종의 관심 뉴클레오티드 서열과 함께 발현 카세트에 제공될 수 있다.
본 발명의 일 양태에서, 발현 카세트는, 형태형성 유전자 및/또는 이종 뉴클레오티드 서열에 작동 가능하게 연결되는, 본 발명의 프로모터 뉴클레오티드 서열 또는 이들의 변이체 또는 단편 중 하나를 포함하는 전사 개시 영역을 포함한다. 이러한 발현 카세트는 조절 영역의 전사 조절하에 있는 뉴클레오티드 서열의 삽입을 위한 복수의 제한 부위를 구비할 수 있다. 발현 카세트는 3' 종결 영역뿐만 아니라 선택 가능한 마커 유전자를 추가로 포함할 수 있다.
발현 카세트는 5'-3' 전사 방향으로, 전사 개시 영역(즉, 본 발명의 프로모터, 또는 이의 변이체 또는 단편), 번역 개시 영역, 이종의 관심 뉴클레오티드 서열, 번역 종결 영역, 및 선택적으로 숙주 유기체에서 기능하는 전사 종결 영역을 포함할 수 있다. 상기 양태의 조절 영역(즉, 프로모터, 전사 조절 영역, 및 번역 종결 영역) 및/또는 폴리뉴클레오티드는 숙주 세포에 대해 또는 서로에 대해 고유/유사한 것일 수 있다. 대안적으로, 상기 양태의 조절 영역 및/또는 폴리뉴클레오티드는 숙주 세포에 대해 또는 서로에 대해 이종성일 수 있다. 본원에 사용된 바와 같이, 서열과 관련하여 "이종성"은 외래종에서 유래된 서열이거나, 또는 동일한 종에서 유래된 경우 의도적인 인간의 개입에 의해 조성물 및/또는 게놈 유전자좌의 고유 형태로부터 실질적으로 변형된 서열이다. 예를 들어, 이종 폴리뉴클레오티드에 작동 가능하게 연결된 프로모터는 폴리뉴클레오티드가 유래된 종과 상이한 종에서 유래되거나, 또는 동일/유사한 종에서 유래된 경우 하나 또는 둘 모두는 원래의 형태 및/또는 게놈 유전자좌로부터 실질적으로 변형되거나 프로모터는 작동 가능하게 연결된 폴리뉴클레오티드에 대한 천연 프로모터가 아니다.
종결 영역은 전사 개시 영역에 고유한 것이거나, 작동 가능하게 연결된 관심 DNA 서열에 고유한 것이거나, 식물 숙주에 고유한 것이거나, 또는 다른 공급원에서 유래된 것일 수 있다(즉, 프로모터, 발현되는 DNA 서열, 식물 숙주, 또는 이들의 임의의 조합에 대해 외래성 또는 이종성). 간편한 종결 영역은 옥토핀 신타제 및 노팔린 신타제 종결 영역과 같은 A. 투메파시엔스의 Ti-플라스미드로부터 이용 가능하다. 또한, 본원에 전문이 참조로 포함되는 문헌[Guerineau, et al., (1991) Mol. Gen. Genet. 262:141-144; Proudfoot, (1991) Cell 64:671-674; Sanfacon, et al., (1991) Genes Dev. 5:141-149; Mogen, et al., (1990) Plant Cell 2:1261-1272; Munroe, et al., (1990) Gene 91:151-158; Ballas, et al., (1989) Nucleic Acids Res. 17:7891-7903; 및 Joshi, et al., (1987) Nucleic Acid Res. 15:9627-9639] 참조.
본 발명의 서열을 포함하는 발현 카세트는 또한, 유기체 내로 공동 형질전환될 적어도 하나의 추가 유전자 뉴클레오티드 서열, 이종 뉴클레오티드 서열, 이종의 관심 폴리뉴클레오티드, 또는 이종 폴리뉴클레오티드를 포함할 수 있다. 대안적으로, 추가의 뉴클레오티드 서열(들)은 다른 발현 카세트에 제공될 수 있다.
적절한 경우, 본 발명의 조직-선호적 프로모터 서열의 제어하에 발현되는 뉴클레오티드 서열, 및 임의의 추가 뉴클레오티드 서열(들)은 형질전환 식물에서의 발현 증가를 위해 최적화될 수 있다. 즉, 이들 뉴클레오티드 서열은 개선된 발현을 위해 식물-선호적 코돈을 사용하여 합성될 수 있다. 숙주-선호적 코돈 사용의 논의에 대해서는, 예를 들어 본원에 전문이 참조로 포함되는 문헌[Campbell and Gowri, (1990) Plant Physiol. 92:1-11] 참조. 식물-선호적 유전자를 합성하기 위한 방법은 당업계에서 이용 가능하다. 예를 들어, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,380,831, 5,436,391 및 문헌[Murray, et al., (1989) Nucleic Acids Res. 17:477-498] 참조.
추가의 서열 변형은 세포 숙주에서 유전자 발현을 향상시키는 것으로 알려져 있다. 이들은 가짜 폴리아데닐화 신호를 암호화하는 서열, 엑손-인트론 스플라이스 부위 신호, 전이인자 유사 반복서열, 및 유전자 발현에 유해할 수 있는 기타 이러한 잘 특성화된 서열의 제거를 포함한다. 이종 뉴클레오티드 서열의 G-C 함량은 숙주 세포에서 발현되는 공지된 유전자를 참조하여 계산되는 주어진 세포 숙주에 대한 평균 수준으로 조정될 수 있다. 가능한 경우, 서열은 예측된 헤어핀 2차 mRNA 구조를 피하도록 변형된다.
발현 카세트는 5' 리더 서열을 추가로 포함할 수 있다. 이러한 리더 서열은 번역을 향상시키는 작용을 할 수 있다. 번역 리더는 당업계에 알려져 있으며, 피코르나바이러스 리더, 예를 들어 EMCV 리더(뇌심근염 5' 비코딩 영역)(Elroy-Stein, et al., (1989) Proc. Nat. Acad. Sci. USA 86:6126-6130); 포티바이러스 리더, 예를 들어 TEV 리더(담배 에칭 바이러스)(Allison, et al., (1986) Virology 154:9-20); MDMV 리더(옥수수 위축 모자이크 바이러스); 인간 면역글로불린 중쇄 결합 단백질(BiP)(Macejak, et al., (1991) Nature 353:90-94); 알팔파 모자이크 바이러스의 외피 단백질 mRNA로부터의 미번역 리더(AMV RNA 4)(Jobling, et al., (1987) Nature 325:622-625); 담배 모자이크 바이러스 리더(TMV)(Gallie, et al., (1989) Molecular Biology of RNA, pages 237-256), 및 옥수수 백화 얼룩 바이러스 리더(MCMV)(Lommel, et al., (1991) Virology 81:382-385)를 제한 없이 포함하며, 이들 문헌의 전문은 본원에 참조로 포함된다. 또한, 본원에 전문이 참조로 포함되는 문헌[Della-Cioppa, et al., (1987) Plant Physiology 84:965-968] 참조. mRNA 안정성을 향상시키는 것으로 알려진 방법, 예를 들어 옥수수 유비퀴틴 인트론(Christensen and Quail, (1996) Transgenic Res. 5:213-218; Christensen, et al., (1992) Plant Molecular Biology 18:675-689) 또는 옥수수 AdhI 인트론(Kyozuka, et al., (1991) Mol. Gen. Genet. 228:40-48; Kyozuka, et al., (1990) Maydica 35:353-357)과 같은 인트론 등이 또한 이용될 수 있으며, 이들 문헌의 전문은 본원에 참조로 포함된다.
상기 양태의 DNA 구축물은 또한, 필요에 따라 인핸서(번역 또는 전사 인핸서)를 추가로 포함할 수 있다. 이러한 인핸서 영역은 당업자에게 잘 알려져 있으며, ATG 개시 코돈 및 인접 서열을 포함할 수 있다. 개시 코돈은 전체 서열의 번역을 보장하기 위해 코딩 서열의 리딩 프레임과 동일한 위상이어야 한다. 번역 제어 신호 및 개시 코돈은 천연 및 합성의 다양한 기원으로부터 유래될 수 있다. 번역 개시 영역은 전사 개시 영역의 공급원로부터, 또는 구조 유전자로부터 제공될 수 있다. 서열은 또한, 유전자를 발현시키도록 선택된 조절 요소로부터 유래될 수 있으며, mRNA의 번역을 증가시키도록 특이적으로 변형될 수 있다. 전사 수준을 높이기 위해 상기 양태의 프로모터 영역과 함께 인핸서가 이용될 수 있는 것으로 인식된다. 인핸서는 당업계에 알려져 있으며, SV40 인핸서 영역, 35S 인핸서 요소 등을 포함한다.
발현 카세트 제조시, DNA 서열을 적절한 방향으로, 적절한 리딩 프레임에서 적절히 제공하기 위해 다양한 DNA 단편이 조작될 수 있다. 이를 위해, 어댑터 또는 링커를 사용하여 DNA 단편일 연결하거나, 간편한 제한 부위, 불필요한 DNA의 제거, 제한 부위의 제거 등을 제공하기 위한 다른 조작이 수반될 수 있다. 이를 위해, 시험관내 돌연변이 유도, 프라이머 복구, 제한, 어닐링, 재치환, 예를 들어 전이 및 전환이 수반될 수 있다.
리포터 유전자 또는 선택 가능한 마커 유전자가 본 발명의 발현 카세트에 또한 포함될 수 있다. 당업계에 알려진 적합한 리포터 유전자의 예는, 예를 들어 본원에 전문이 참조로 포함되는 문헌[Jefferson, et al., (1991), Plant Molecular Biology Manual, ed. Gelvin, et al., (Kluwer Academic Publishers), pp. 1-33; DeWet, et al., (1987) Mol. Cell. Biol. 7:725-737; Goff, et al., (1990) EMBO J. 9:2517-2522; Kain, et al., (1995) Bio Techniques 19:650-655 및 Chiu, et al., (1996) Current Biology 6:325-330]에서 확인할 수 있다.
형질전환 세포 또는 조직의 선별을 위한 선택 가능한 마커 유전자는 항생제 저항성 또는 제초제에 대한 저항성을 부여하는 유전자를 포함할 수 있다. 적합한 선택 가능한 마커 유전자의 예는 클로람페니콜에 대한 저항성을 암호화하는 유전자(Herrera Estrella, et al., (1983) EMBO J. 2:987-992); 메토트렉세이트(Herrera Estrella, et al., (1983) Nature 303:209-213; Meijer, et al., (1991) Plant Mol. Biol. 16:807-820); 히그로마이신(Waldron, et al., (1985) Plant Mol. Biol. 5:103-108 및 Zhijian, et al., (1995) Plant Science 108:219-227); 스트렙토마이신(Jones, et al., (1987) Mol. Gen. Genet. 210:86-91); 스펙티노마이신(Bretagne-Sagnard, et al., (1996) Transgenic Res. 5:131-137); 블레오마이신(Hille, et al., (1990) Plant Mol. Biol. 7:171-176); 설폰아미드(Guerineau, et al., (1990) Plant Mol. Biol. 15:127-36); 브로목시닐(Stalker, et al., (1988) Science 242:419-423); 글리포세이트(Shaw, et al., (1986) Science 233:478-481 및 미국 특허 출원 일련번호 10/004,357 및 10/427,692); 포스피노트리신(DeBlock, et al., (1987) EMBO J. 6:2513-2518)을 포함하나, 이에 한정되지 않으며, 이들 문헌의 전문은 본원에 참조로 포함된다.
유전자이식 이벤트의 재생에 유용할 수 있는 다른 유전자는 GUS(베타-글루쿠로니다제; Jefferson, (1987) Plant Mol. Biol. Rep. 5:387), GFP(녹색 형광 단백질; Chalfie, et al., (1994) Science 263:802), 루시퍼라제(Riggs, et al., (1987) Nucleic Acids Res. 15(19):8115 및 Luehrsen, et al., (1992) Methods Enzymol. 216:397-414) 및 안토시아닌 생성을 암호화하는 옥수수 유전자(Ludwig, et al., (1990) Science 247:449)와 같은 예를 포함하나, 이에 한정되지 않으며, 이들 문헌의 전문은 본원에 참조로 포함된다.
형태형성 유전자에 작동 가능하게 연결되고, 선택적으로 이종 뉴클레오티드 서열, 이종의 관심 폴리뉴클레오티드, 이종 폴리뉴클레오티드 뉴클레오티드, 또는 관심 서열에 추가로 작동 가능하게 연결되는 본 발명의 프로모터 서열을 포함하는 발현 카세트는 임의의 식물을 형질전환시키는 데 사용될 수 있다. 이러한 방식으로, 유전자 변형된 식물, 식물 세포, 식물 조직, 종자, 뿌리 등을 얻을 수 있다.
본원에 사용된 바와 같이, "벡터"는 숙주 세포에 뉴클레오티드 구축물, 예를 들어 발현 카세트 또는 구축물을 도입하기 위한 플라스미드, 코스미드, 또는 박테리아 파지와 같은 DNA 분자를 지칭한다. 클로닝 벡터는 일반적으로, 외래 DNA 서열이 벡터의 본질적인 생물학적 기능의 손실 없이 확인 가능한 방식으로 삽입될 수 있는 하나 또는 소수의 제한 엔도뉴클레아제 인식 부위, 뿐만 아니라 클로닝 벡터로 형질전환된 세포의 동정 및 선별에 사용하기에 적합한 마커 유전자를 포함한다. 마커 유전자는 일반적으로, 테트라사이클린 저항성, 히그로마이신 저항성, 또는 암피실린 저항성을 제공하는 유전자를 포함한다.
본 발명의 방법은 폴리펩티드 또는 폴리뉴클레오티드를 식물에 도입하는 것을 수반한다. 본원에 사용된 바와 같이, "도입"은 식물 세포의 내부로 서열이 접근하는 방식으로 식물에 폴리뉴클레오티드 또는 폴리펩티드를 제공하는 것을 의미한다. 본 발명의 방법은 폴리뉴클레오티드 또는 폴리펩티드가 식물의 적어도 하나의 세포의 내부로 접근하는 한, 식물에 서열을 도입하기 위한 특정 방법에 의존하지 않는다. 폴리뉴클레오티드 또는 폴리펩티드를 식물에 도입하는 방법은 당업계에 알려져 있으며, 안정적인 형질전환 방법, 일시적 형질전환 방법, 및 바이러스-매개 방법을 포함하나, 이에 한정되지 않는다.
"안정적인 형질전환"은 식물에 도입된 뉴클레오티드 구축물이 식물의 게놈에 통합되어 자손에 유전될 수 있는 형질전환이다. "일시적 형질전환"은 폴리뉴클레오티드가 식물에 도입되고 식물의 게놈에 통합되지 않거나 폴리펩티드가 식물에 도입되는 것을 의미한다.
형질전환 프로토콜뿐만 아니라 뉴클레오티드 서열을 식물에 도입하기 위한 프로토콜은 형질전환 대상 식물 또는 식물 세포의 유형(즉, 외떡잎식물 또는 쌍떡잎식물)에 따라 달라질 수 있다. 뉴클레오티드 서열을 식물 세포에 도입하고 후속적으로 식물 게놈에 삽입하는 적합한 방법은 마이크로주입(Crossway, et al., (1986) Biotechniques 4:320-334), 전기천공법(Riggs, et al., (1986) Proc. Natl. Acad. Sci. USA 83:5602-5606), 아그로박테리움-매개 형질전환(Townsend, et al., 미국 특허 번호 5,563,055 및 Zhao, et al., 미국 특허 번호 5,981,840), 유전자 직접전달(Paszkowski, et al., (1984) EMBO J. 3:2717-2722), 탄도 입자 가속(예를 들어, 미국 특허 번호 4,945,050; 5,879,918; 5,886,244; 5,932,782; 문헌[Tomes, et al., (1995), Plant Cell, Tissue, and Organ Culture: Fundamental Methods, ed. Gamborg and Phillips (Springer-Verlag, Berlin); McCabe, et al., (1988) Biotechnology 6:923-926)] 참조), 및 Lec1 형질전환(WO 00/28058)을 포함한다. 또한, 본원에 전문이 참조로 포함되는 문헌[Weissinger, et al., (1988) Ann. Rev. Genet. 22:421-477; Sanford, et al., (1987) Particulate Science and Technology 5:27-37(양파); Christou, et al., (1988) Plant Physiol. 87:671-674(대두); McCabe, et al., (1988) Bio/Technology 6:923-926(대두); Finer and McMullen, (1991) In Vitro Cell Dev. Biol. 27P:175-182(대두); Singh, et al., (1998) Theor. Appl. Genet. 96:319-324(대두); Datta, et al., (1990) Biotechnology 8:736-740(벼); Klein, et al., (1988) Proc. Natl. Acad. Sci. USA 85:4305-4309(옥수수); Klein, et al., (1988) Biotechnology 6:559-563(옥수수)]; 미국 특허 번호 5,240,855; 5,322,783 및 5,324,646; 문헌[Klein, et al., (1988) Plant Physiol. 91:440-444(옥수수); Fromm, et al., (1990) Biotechnology 8:833-839(옥수수); Hooykaas-Van Slogteren, et al., (1984) Nature (London) 311:763-764]; 미국 특허 번호 5,736,369(곡물); 문헌[Bytebier, et al., (1987) Proc. Natl. Acad. Sci. USA 84:5345-5349(백합과); De Wet, et al., (1985), The Experimental Manipulation of Ovule Tissues, ed. Chapman, et al., (Longman, New York), pp. 197-209(꽃가루); Kaeppler, et al., (1990) Plant Cell Reports 9:415-418 및 Kaeppler, et al., (1992) Theor. Appl. Genet. 84:560-566(휘스커-매개 형질전환); D'Halluin, et al., (1992) Plant Cell 4:1495-1505(전기천공법); Li, et al., (1993) Plant Cell Reports 12:250-255 및 Christou and Ford, (1995) Annals of Botany 75:407-413(벼); Osjoda, et al., (1996) Nature Biotechnology 14:745-750(아그로박테리움 투메파시엔스를 통한 옥수수)] 참조. 빠른 식물 형질전환을 위한 방법 및 조성물은 본원에 전문이 참조로 포함되는 U.S. 2017/0121722에서 또한 확인된다. 식물 형질전환에 유용한 벡터는 본원에 전문이 참조로 포함되는 미국 특허 출원 일련번호 15/765,521에서 확인된다.
특정 양태에서, 본 발명의 프로모터 서열을 포함하는 DNA 구축물은 다양한 일시적 형질전환 방법을 이용해 식물에 제공될 수 있다. 이러한 일시적 형질전환 방법은 바이러스 벡터 시스템, 및 DNA의 후속 방출을 배제하는 방식으로 폴리뉴클레오티드를 침전시키는 것을 포함하나, 이에 한정되지 않는다. 따라서, 입자 결합 DNA로부터 전사가 일어날 수 있지만, DNA가 방출되어 게놈에 통합되는 빈도는 크게 줄어든다. 이러한 방법은 폴리에틸이민(PEI; Sigma #P3143)으로 코팅된 입자의 사용을 포함한다.
다른 양태에서, 본 발명의 폴리뉴클레오티드는 식물을 바이러스 또는 바이러스 핵산과 접촉시켜 식물에 도입될 수 있다. 일반적으로, 이러한 방법은 본 발명의 뉴클레오티드 구축물을 바이러스 DNA 또는 RNA 분자 내에 혼입시키는 것을 수반한다. 폴리뉴클레오티드를 식물에 도입하고, 바이러스 DNA 또는 RNA 분자를 포함하여 그 안에 암호화된 단백질을 발현시키는 방법은 당업계에 알려져 있다. 예를 들어, 본원에 전문이 참조로 포함되는 미국 특허 번호 5,889,191, 5,889,190, 5,866,785, 5,589,367, 5,316,931 및 문헌[Porta, et al., (1996) Molecular Biotechnology 5:209-221] 참조.
폴리뉴클레오티드를 식물 게놈의 특정 위치에 표적화 삽입하는 방법은 당업계에 알려져 있다. 일 양태에서, 폴리뉴클레오티드를 원하는 게놈 위치에 삽입하는 것은 부위-특이적 재조합 시스템을 이용하여 달성된다. 예를 들어, 본원에 전문이 참조로 포함되는 WO99/25821, WO99/25854, WO99/25840, WO99/25855, 및 WO99/25853 참조. 간략히 요약하면, 본 발명의 폴리뉴클레오티드는 2개의 동일하지 않은 재조합 부위로 플랭킹된 전달 카세트에 포함될 수 있다. 전달 카세트는 전달 카세트의 부위에 해당하는 2개의 동일하지 않은 재조합 부위로 플랭킹된 표적 부위가 게놈에 안정적으로 통합된 식물에 도입된다. 적절한 재조합효소가 제공되고 전달 카세트는 표적 부위에 통합된다. 이에 따라 관심 폴리뉴클레오티드는 식물 게놈의 특정 염색체 위치에 통합된다.
형질전환된 세포는 통상적인 방식에 따라 식물로 성장될 수 있다. 예를 들어, 본원에 전문이 참조로 포함되는 문헌[McCormick, et al., (1986) Plant Cell Reports 5:81-84] 참조. 이후, 이들 식물은 성장되고, 동일한 형질전환 균주 또는 상이한 균주로 수분되어, 원하는 표현형 특성이 발현된 자손을 얻을 수 있다. 원하는 표현형 특성의 발현이 안정적으로 유지되고 유전되도록 하기 위해 2대 이상에 걸쳐 성장될 수 있고, 이후 원하는 표현형 특성의 발현이 달성되었음을 확실히 하기 위해 종자가 수확된다. 이러한 방식으로, 본 발명은 본 발명의 뉴클레오티드 구축물, 예를 들어 본 발명의 발현 카세트가 게놈에 안정적으로 통합된 형질전환 종자("유전자이식 종자"라고도 함)를 제공한다.
식물 조직으로부터 식물을 재생하기 위한 다양한 방법이 있다. 특정 재생 방법은 출발 식물 조직 및 재생될 특정 식물 종에 따라 달라질 것이다. 단일의 식물 원형질 형질전환체 또는 다양한 형질전환 외식편으로부터의 식물의 재생, 발육, 및 배양은 당업계에 잘 알려져 있다(본원에 전문이 참조로 포함되는 문헌[Weissbach and Weissbach, (1988), Methods for Plant Molecular Biology, (Eds.), Academic Press, Inc., San Diego, Calif.]). 이러한 재생 및 성장 과정은 일반적으로, 형질전환 세포를 선별하고, 개별화된 세포를 통상적인 배발육기를 통해 발근 묘목기를 통해 배양하는 단계를 포함한다. 유전자이식 배아와 종자는 유사하게 재생된다. 이후, 생성된 유전자이식 발근된 싹을 토양과 같은 적절한 식물 성장 배지에 심는다. 바람직하게, 재생 식물은 동형접합 유전자이식 식물을 제공하기 위해 자가 수분된다. 그렇지 않으면, 재생 식물로부터 얻은 꽃가루를 작물학적으로 중요한 계통의 종자 식물과 교배한다. 반대로, 이러한 중요한 계통의 식물에서 나온 꽃가루는 재생 식물을 수분시키기 위해 사용된다. 원하는 폴리뉴클레오티드를 함유하는 상기 양태의 유전자이식 식물은 당업자에게 잘 알려진 방법을 이용해 배양된다.
상기 양태는 식물 내에서 발현을 조절하는 화합물을 스크리닝하기 위한 조성물을 제공한다. 벡터, 세포, 및 식물은 본원에 개시된 조절 서열의 작용제 및 길항제를 위한 후보 분자를 스크리닝하는 데 사용될 수 있다. 예를 들어, 리포터 유전자가 조절 서열에 작동 가능하게 연결되어 식물에서 이식유전자로서 발현될 수 있다. 시험될 화합물을 첨가하고 리포터 유전자 발현을 측정하여 프로모터 활성에 대한 영향을 확인한다.
일 양태에서, 개시된 방법 및 조성물은 체세포배로부터 유도된 식물의 게놈에서의 변형을 위한 특정 부위를 표적화하는 데 유용한 폴리뉴클레오티드를 증가된 효율과 속도로 체세포배에 도입하기 위해 사용될 수 있다. 개시된 방법 및 조성물로 도입될 수 있는 부위-특이적 변형은 부위-특이적 변형을 도입하기 위한 임의의 방법(유전자 복구 올리고뉴클레오티드의 사용(예를 들어, 미국 공보 2013/0019349), 또는 TALEN, 메가뉴클레아제, 징크 핑거 뉴클레아제, CRISPR-Cas 등과 같은 이중가닥 절단 기술의 사용을 포함하나, 이에 한정되지 않음)을 이용해 생성된 것들을 포함한다. 예를 들어, 개시된 방법 및 조성물은 식물 또는 식물 세포의 게놈에서 표적 서열의 게놈 변형을 위해, 식물 선별을 위해, 염기 또는 서열의 제거를 위해, 유전자 편집을 위해, 식물 또는 식물 세포의 게놈에 관심 폴리뉴클레오티드를 삽입하기 위해, 식물 세포 또는 식물에 CRISPR-Cas 시스템을 도입하는 데 사용될 수 있다. 따라서, 개시된 방법 및 조성물은 식물, 식물 세포 또는 종자의 게놈 내 표적 부위 및 관심 뉴클레오티드의 변형 또는 변경을 위한 효과적인 시스템을 제공하기 위해 CRISPR-Cas 시스템과 함께 사용될 수 있다. Cas 엔도뉴클레아제 유전자는 식물 최적화 Cas9 엔도뉴클레아제이며, 식물 최적화 Cas9 엔도뉴클레아제는 식물 게놈의 게놈 표적 서열에 결합하여 이중가닥 절단을 생성할 수 있다.
Cas 엔도뉴클레아제는 특정 표적 부위에서 이중가닥 절단을 인식하여 선택적으로 세포의 게놈에 도입하도록 가이드 뉴클레오티드에 의해 가이드된다. CRISPR-Cas 시스템은 식물, 식물 세포 또는 종자의 게놈 내 표적 부위의 변형을 위한 효과적인 시스템을 제공한다. 세포의 게놈 내 표적 부위의 변형 및 세포의 게놈 내 뉴클레오티드 서열의 편집을 위한 효과적인 시스템을 제공하기 위해 가이드 폴리뉴클레오티드/Cas 엔도뉴클레아제 시스템을 이용하는 방법 및 조성물이 또한 제공된다. 게놈 표적 부위가 확인되면, 다양한 관심 폴리뉴클레오티드를 함유하도록 표적 부위를 추가로 변형시키기 위해 다양한 방법이 사용될 수 있다. 개시된 조성물 및 방법은 세포의 게놈 내 뉴클레오티드 서열의 편집을 위한 CRISPR-Cas 시스템을 도입하는 데 사용될 수 있다. 편집될 뉴클레오티드 서열(관심 뉴클레오티드 서열)은 Cas 엔도뉴클레아제에 의해 인식되는 표적 부위 내에 또는 밖에 위치할 수 있다.
CRISPR 유전자좌(일정한 간격을 두고 규칙적으로 분포하는 짧은 회문 반복서열)(SPIDR(SPacer Interspersed Direct Repeats)로도 알려져 있음)는 최근에 기술된 DNA 유전자좌의 계열을 구성한다. CRISPR 유전자좌는 부분적으로 회문구조인, 짧고 고도로 보존된 DNA 반복서열(일반적으로 24 내지 40 bp, 1 내지 140회 반복되며, CRISPR 반복서열이라고도 함)로 이루어진다. (일반적으로 종에 특이적인) 반복 서열이 일정한 길이(CRISPR 유전자좌에 따라 일반적으로 20 내지 58 bp)의 가변 서열을 사이에 두고 분포된다(2007년 3월 1일 공개된 WO2007/025097).
CRISPR 유전자좌는 대장균에서 처음 인식되었다(Ishino et al. (1987) J. Bacterial. 169:5429-5433; Nakata et al. (1989) J. Bacterial. 171 :3553-3556). 유사한 산재형 짧은 서열 반복이 문헌[Haloferax mediterranei, Streptococcus pyogenes, Anabaena, and Mycobacterium tuberculosis (Groenen et al. (1993) Mol. Microbiol. 10:1057-1065; Hoe et al. (1999) Emerg. Infect. Dis. 5:254- 263; Masepohl et al. (1996) Biochim. Biophys. Acta 1307:26-30; Mojica et al. (1995) Mol. Microbiol. 17:85-93)]에서 확인되었다. CRISPR 유전자좌는 다른 SSR과는 반복서열의 구조가 상이해, 일정한 간격을 두고 규칙적으로 분포하는 짧은 반복서열(SRSR)로 명명되었다(Janssen et al. (2002) OMICS J. Integ. Biol. 6:23-33; Mojica et al. (2000) Mol. Microbiol. 36:244-246). 반복서열은 클러스터에서 발생하는 짧은 요소로서, 항상 일정한 길이의 가변 서열을 사이에 두고 규칙적으로 분포한다(Mojica et al. (2000) Mol. Microbiol. 36:244-246).
Cas 유전자는, 일반적으로 플랭킹 CRISPR 유전자좌에 커플링되거나 결합되거나 가까이 있거나, 또는 그 부근에 있는 유전자를 포함한다. 용어 "Cas 유전자" 및 "CRISPR-결합 (Cas) 유전자"는 본원에서 상호교환적으로 사용된다. Cas 단백질 계열에 대한 포괄적인 리뷰는 문헌[Haft et al. (2005) Computational Biology, PLoS Comput Biol 1 (6): e60. doi:10.1371/journal.pcbi.0010060]에 제공된다.
초기에 기술된 4개의 유전자 계열 외에, 추가 41개의 CRISPR-결합 (Cas) 유전자 계열이, 본원에 참조로 포함되는 WO/2015/026883에 기술되었다. 이 문헌은 CRISPR 시스템이 상이한 반복 패턴, 유전자 세트, 및 종 범위를 갖는 상이한 부류에 속함을 나타낸다. 주어진 CRISPR 유전자좌에서 Cas 유전자의 수는 종에 따라 다를 수 있다. Cas 엔도뉴클레아제는 Cas 유전자에 의해 암호화된 Cas 단백질과 관련되며, Cas 단백질은 DNA 표적 서열에 이중가닥 절단을 도입할 수 있다. Cas 엔도뉴클레아제는 특정 표적 부위에서 이중가닥 절단을 인식하여 선택적으로 세포의 게놈에 도입하도록 가이드 폴리뉴클레오티드에 의해 가이드된다. 본원에 사용된 용어 "가이드 폴리뉴클레오티드/Cas 엔도뉴클레아제 시스템"은 DNA 표적 서열에 이중가닥 절단을 도입할 수 있는 Cas 엔도뉴클레아제와 가이드 폴리뉴클레오티드의 복합체를 포함한다. Cas 엔도뉴클레아제는 게놈 표적 부위에 아주 근접하여 DNA 듀플렉스를 풀고, 가이드 뉴클레오티드가 표적 서열을 인식하면(단, 정확한 프로토스페이서 인접 모티프(PAM)의 방향이 대략 표적 서열의 3' 말단을 향하는 경우에만) 두 DNA 가닥을 절단한다(2015년 2월 26일 공개된 WO/2015/02688의 도 2A 및 도 2B 참조).
일 양태에서, Cas 엔도뉴클레아제 유전자는 Cas9 엔도뉴클레아제(예컨대, 이에 한정되는 것은 아니지만, 본원에 참조로 포함되는 WO2007/025097(2007년 3월 1일 공개)의 서열 번호 462, 474, 489, 494, 499, 505, 및 518에 나열된 Cas9 유전자)이다. 다른 양태에서, Cas 엔도뉴클레아제 유전자는 식물, 옥수수 또는 대두 최적화 Cas9 엔도뉴클레아제(예컨대, 이에 한정되는 것은 아니지만 WO/2015/026883의 도 1A에 표시된 것들)이다. 다른 양태에서, Cas 엔도뉴클레아제 유전자는 Cas 코돈 영역 상류의 SV40 핵 표적화 신호 및 Cas 코돈 영역 하류의 2부분 VirD2 핵 국재화 신호(Tinland et al. (1992) Proc. Natl. Acad. Sci. USA 89:7442-6)에 작동 가능하게 연결된다.
일 양태에서, Cas 엔도뉴클레아제 유전자는 WO/2015/026883의 서열 번호 1, 124, 212, 213, 214, 215, 216, 193 또는 서열 번호 5의 뉴클레오티드 2037-6329, 또는 이들의 임의의 기능적 단편 또는 변이체의 Cas9 엔도뉴클레아제 유전자이다.
Cas 엔도뉴클레아제와 관련하여, 용어 "기능적 단편", "기능적으로 동등한 단편", 및 "기능적 동등 단편"은 본원에서 상호교환적으로 사용된다. 이들 용어는 이중가닥 절단을 생성하는 능력이 유지된, 본 발명의 Cas 엔도뉴클레아제 서열의 일부 또는 하위서열을 지칭한다.
Cas 엔도뉴클레아제와 관련하여, 용어 "기능적 변이체", "기능적으로 동등한 변이체", 및 "기능적 동등 변이체"는 본원에서 상호교환적으로 사용된다. 이들 용어는 이중가닥 절단을 생성하는 능력이 유지된, 본 발명의 Cas 엔도뉴클레아제의 변이체를 지칭한다. 단편 및 변이체는 부위-특이적 돌연변이 유도 및 합성 제조와 같은 방법을 통해 얻을 수 있다.
일 양태에서, Cas 엔도뉴클레아제 유전자는 원칙적으로 표적화될 수 있는 N(12~30)NGG 형태의 임의의 게놈 서열을 인식할 수 있는 식물 코돈 최적화 Streptococcus pyogenes Cas9 유전자이다.
엔도뉴클레아제는 폴리뉴클레오티드 사슬 내 포스포디에스테르 결합을 절단하는 효소로서, 염기를 손상시키지 않고 특정 부위에서 DNA를 절단하는 제한 엔도뉴클레아제를 포함한다. 제한 엔도뉴클레아제는 I형, II형, III형, 및 IV형 엔도뉴클레아제를 포함하고, 이들은 하위유형을 추가로 포함한다. I형 및 III형 시스템에서, 메틸라제 활성과 제한 활성 모두 단일 복합체에 포함된다. 엔도뉴클레아제는 호밍 엔도뉴클레아제(HEase)로도 알려진 메가뉴클레아제도 포함하는데, 이는 제한 엔도뉴클레아제처럼 특정 인식 부위에서 결합하고 절단하지만, 메가뉴클레아제에 대한 인식 부위는 약 18 bp 이상으로 일반적으로 더 길다(2012년 3월 22일 출원된 특허 출원 PCT/US 12/30061). 메가뉴클레아제는 보존된 서열 모티프에 기초하여 4개의 계열로 분류되었는데, 그 계열은 LAGLIDADG, GIY-YIG, H-N-H, 및 His-Cys 박스 계열이다. 이들 모티프는 금속 이온의 배위 및 포스포디에스테르 결합의 가수분해에 참여한다. 메가뉴클레아제는 긴 인식 부위, 및 이의 DNA 기질에서 일부 서열 다형성을 용인하는 것으로 유명하다. 메가뉴클레아제에 대한 명명 규칙은 다른 제한 엔도뉴클레아제에 대한 규칙과 유사하다. 메가뉴클레아제는 또한, 독립형 ORF, 인트론, 및 인테인에 의해 각각 암호화되는 효소에 대한 접두사 F-, I-, 또는 PI-를 특징으로 한다. 재조합 프로세스에서 하나의 단계는 인식 부위 또는 그 근처에서의 폴리뉴클레오티드 절단을 수반한다. 이 절단 활성은 이중가닥 절단을 생성하는 데 이용될 수 있다. 부위-특이적 재조합효소 및 그 인식 부위에 대한 리뷰에 대해서는 문헌[Sauer (1994) Curr Op Biotechnol 5:521 -7; 및 Sadowski (1993) FASEB 7:760-7] 참조. 일부 예에서, 재조합효소는 인테그라제 또는 레솔바제 계열에 속한다. TAL 이펙터 뉴클레아제는 식물 또는 다른 생물의 게놈 내 특정 표적 서열에서 이중가닥 절단을 만들기 위해 사용될 수 있는 새로운 부류의 서열-특이적 뉴클레아제이다(Miller, et al. (2011) Nature Biotechnology 29:143-148). 징크 핑거 뉴클레아제(ZFN)는 징크 핑거 DNA 결합 도메인 및 이중가닥 절단 유도제 도메인으로 이루어진 조작된 이중가닥 절단 유도제이다. 인식 부위 특이성은 징크 핑커 도메인에 의해 부여되며, 이는 일반적으로 2, 3, 또는 4개의 징크 핑거를 포함하고, 예를 들어, C2H2 구조를 갖지만, 다른 징크 핑거 구조는 알려져 있고 유전자 조작되었다. 징크 핑거 도메인은 선택된 폴리뉴클레오티드 인식 서열에 특이적으로 결합하는 폴리펩티드를 설계하는 데 쉽게 이용될 수 있다. ZFN은 비특이적 엔도뉴클레아제 도메인, 예를 들어, FokI와 같은 Ms형 엔도뉴클레아제로부터의 뉴클레아제 도메인에 연결된 조작된 DNA-결합 징크 핑거 도메인을 포함한다. 전사 활성물질 도메인, 전사 억제물질 도메인, 및 메틸라제를 포함하여, 추가 기능이 징크 핑거 결합 도메인에 융합될 수 있다. 일부 예에서, 절단 활성을 위해 뉴클레아제 도메인의 이합체화가 요구된다. 각각의 징크 핑거는 표적 DNA에서 3개의 연속 염기쌍을 인식한다. 예를 들어, 뉴클레아제의 이합체화 요건하에, 3-핑거 도메인은 9개 연속 뉴클레오티드의 서열을 인식하고, 18-뉴클레오티드 인식 서열을 결합시키는 데 두 세트의 징크 핑거 트리플렛이 사용된다.
박테리아 및 고세균은, 짧은 RNA를 이용해 외래 핵산의 분해를 유도하는, 일정한 간격을 두고 규칙적으로 분포하는 짧은 회문 반복서열(CRISPR)/CRISPR-결합 (Cas) 시스템이라고 하는 적응 면역 방어를 진화시켰다(2007년 3월 1일 공개된 WO2007/025097). 박테리아로부터의 II형 CRISPR/Cas 시스템은 crRNA 및 tracrRNA를 사용하여 Cas 엔도뉴클레아제를 DNA 표적으로 가이드한다. crRNA(CRISPR RNA)는 이중가닥 DNA 표적의 한 가닥에 상보적인 영역 및 Cas 엔도뉴클레아제가 DNA 표적을 절단하도록 유도하는 RNA 듀플렉스를 형성하는 tracrRNA(트랜스-활성화 CRISPR RNA)와의 염기쌍을 포함한다.
본원에 사용된 용어 "가이드 뉴클레오티드"는 2개의 RNA 분자, 가변 표적화 도메인을 포함하는 crRNA(CRISPR RNA), 및 tracrRNA의 합성 융합체에 관한 것이다. 일 양태에서, 가이드 뉴클레오티드는 12 내지 30개 뉴클레오티드 서열의 가변 표적화 도메인, 및 Cas 엔도뉴클레아제와 상호작용할 수 있는 RNA 단편을 포함한다.
본원에 사용된 용어 "가이드 폴리뉴클레오티드"는, Cas 엔도뉴클레아제와 복합체를 형성할 수 있고 Cas 엔도뉴클레아제가 DNA 표적 부위를 인식하고 선택적으로 절단할 수 있게 하는 폴리뉴클레오티드 서열에 관한 것이다. 가이드 폴리뉴클레오티드는 단일 분자 또는 이중 분자일 수 있다. 가이드 폴리뉴클레오티드 서열은 RNA 서열, DNA 서열, 또는 이들의 조합(RNA-DNA 조합 서열)일 수 있다. 선택적으로, 가이드 폴리뉴클레오티드는 적어도 하나의 뉴클레오티드, 포스포디에스테르 결합 또는 연결 변형, 예컨대 고정 핵산(LNA), 5-메틸 dC, 2,6-디아미노퓨린, 2'-플루오로 A, 2'-플루오로 U, 2'-O-메틸 RNA, 포스포로티오에이트 결합, 콜레스테롤 분자에 대한 연결, 폴리에틸렌 글리콜 분자에 대한 연결, 스페이서 18(헥사에틸렌 글리콜 사슬) 분자에 대한 연결, 또는 고리화를 초래하는 5'에서 3'으로의 공유 연결을 포함할 수 있으나, 이에 한정되지 않는다. 단독으로 리보핵산을 포함하는 가이드 폴리뉴클레오티드는 "가이드 뉴클레오티드"라고도 지칭된다.
가이드 폴리뉴클레오티드는 표적 DNA의 뉴클레오티드 서열에 상보적인 제1 뉴클레오티드 서열(가변 표적화 도메인 또는 VT 도메인으로 지칭됨) 및 Cas 엔도뉴클레아제 폴리펩티드와 상호작용하는 제2 뉴클레오티드 서열 도메인(Cas 엔도뉴클레아제 인식 도메인 또는 CER 도메인으로 지칭됨)을 포함하는 이중 분자(듀플렉스 가이드 폴리뉴클레오티드라고도 지칭됨)일 수 있다. 이중 분자 가이드 폴리뉴클레오티드의 CER 도메인은 상보성 영역을 따라 혼성화된 2개의 별개의 분자를 포함한다. 2개의 별개의 분자는 RNA, DNA, 및/또는 RNA-DNA- 조합 서열일 수 있다. 일 양태에서, CER 도메인에 연결된 VT 도메인을 포함하는 듀플렉스 가이드 폴리뉴클레오티드의 제1 분자는 "crDNA"(연속된 DNA 뉴클레오티드들로 구성되는 경우) 또는 "crRNA"(연속된 RNA 뉴클레오티드들로 구성되는 경우), 또는 "crDNA-RNA"(DNA와 RNA 뉴클레오티드의 조합으로 구성되는 경우)로 지칭된다. cr뉴클레오티드는 박테리아 및 고세균에서 자연적으로 발생하는 cRNA의 단편을 포함할 수 있다. 일 양태에서, 박테리아 및 고세균류에서 자연적으로 발생하고 본원에 개시된 cr뉴클레오티드에 존재하는 cRNA의 단편의 크기는 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20개 이상의 뉴클레오티드 범위일 수 있지만, 이들 범위로 제한되는 것은 아니다.
일 양태에서, CER 도메인을 포함하는 듀플렉스 가이드 폴리뉴클레오티드의 제2 분자는 "tracrRNA"(연속된 RNA 뉴클레오티드들로 구성되는 경우) 또는 "tracrDNA"(연속된 DNA 뉴클레오티드들로 구성되는 경우) 또는 "tracrDNA-RNA"(DNA와 RNA 뉴클레오티드의 조합으로 구성되는 경우)로 지칭된다. 일 양태에서, RNA Cas9 엔도뉴클레아제 복합체를 가이드하는 RNA는 듀플렉스 crRNA-tracrRNA를 포함하는 듀플렉스화된 RNA이다.
또한, 가이드 폴리뉴클레오티드는 표적 DNA의 뉴클레오티드 서열에 상보적인 (가변 표적화 도메인 또는 VT 도메인으로 지칭되는) 제1 뉴클레오티드 서열 도메인 및 Cas 엔도뉴클레아제 폴리펩티드와 상호작용하는 (Cas 엔도뉴클레아제 인식 도메인 또는 CER 도메인으로 지칭되는) 제2 뉴클레오티드 도메인을 포함하는 단일 분자일 수 있다. "도메인"은 RNA, DNA, 및/또는 RNA-DNA 조합 서열일 수 있는 뉴클레오티드들이 연속되어 있는 것을 의미한다. 단일 가이드 폴리뉴클레오티드의 VT 도메인 및/또는 CER 도메인은 RNA 서열, DNA 서열, 또는 RNA-DNA-조합 서열을 포함할 수 있다. 일 양태에서, 단일 가이드 폴리뉴클레오티드는 (CER 도메인을 포함하는) tracr뉴클레오티드에 연결된 (CER 도메인에 연결된 VT 도메인을 포함하는) cr뉴클레오티드를 포함하며, 이러한 연결은 RNA 서열 DNA 서열, 또는 RNA- DNA 조합 서열을 포함하는 뉴클레오티드 서열이다. cr뉴클레오티드 및 tracr뉴클레오티드로부터의 서열들로 구성되는 단일 가이드 폴리뉴클레오티드는 (연속된 RNA 뉴클레오티드들로 구성되는 경우) "단일 가이드 뉴클레오티드" 또는 (연속된 DNA 뉴클레오티드들로 구성되는 경우) "단일 가이드 DNA" 또는 (RNA와 DNA 뉴클레오티드의 조합으로 구성되는 경우) "단일 가이드 뉴클레오티드-DNA"로 지칭될 수 있다. 본 발명의 일 양태에서, 단일 가이드 뉴클레오티드는 II형 Cas 엔도뉴클레아제와 복합체를 형성할 수 있는 II형 CRISPR/Cas 시스템의 cRNA 또는 cRNA 단편 및 tracrRNA 또는 tracrRNA 단편을 포함하고, 여기서 가이드 뉴클레오티드 Cas 엔도뉴클레아제 복합체는 Cas 엔도뉴클레아제를 식물 게놈 표적 부위로 유도하여, Cas 엔도뉴클레아제가 게놈 표적 부위에 이중가닥 절단을 도입할 수 있게 한다. 듀플렉스 가이드 폴리뉴클레오티드 대비 단일 가이드 폴리뉴클레오티드를 사용하는 일 양태는, 단일 가이드 폴리뉴클레오티드를 발현시키기 위해 하나의 발현 카세트만 제조될 필요가 있다는 것이다.
용어 "가변 표적화 도메인" 또는 "VT 도메인"은 본원에서 상호교환적으로 사용되며, 이중가닥 DNA 표적 부위의 한 가닥(뉴클레오티드 서열)에 상보적인 뉴클레오티드 서열을 포함한다. 제1 뉴클레오티드 서열 도메인(VT 도메인)과 표적 서열 간의 상보성 백분율은 적어도 50%, 51 %, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61 %, 62%, 63%, 63%, 65%, 66%, 67%, 68%, 69%, 70%, 71 %, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81 %, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 100%일 수 있다. 가변 표적화 도메인은 적어도 12개, 13개, 14개, 15개, 16개, 17개, 18개, 19개, 20개, 21개, 22개, 23개, 24개, 25개, 26개, 27개, 28개, 29개 또는 30개의 뉴클레오티드 길이일 수 있다. 일 양태에서, 가변 표적화 도메인은 연속된 12개 내지 30개의 뉴클레오티드를 포함한다. 가변 표적화 도메인은 DNA 서열, RNA 서열, 변형된 DNA 서열, 변형된 RNA 서열, 또는 이들의 임의의 조합으로 구성될 수 있다.
가이드 폴리뉴클레오티드의 "Cas 엔도뉴클레아제 인식 도메인" 또는 "CER 도메인"이란 용어는 본원에서 상호교환적으로 사용되며, Cas 엔도뉴클레아제 폴리펩티드와 상호작용하는 뉴클레오티드 서열(예컨대, 가이드 폴리뉴클레오티드의 제2 뉴클레오티드 서열 도메인)을 포함한다. CER 도메인은 DNA 서열, RNA 서열, 변형된 DNA 서열, 변형된 RNA 서열(예컨대, 본원에 기술된 변형 참조), 또는 이들의 임의의 조합으로 구성될 수 있다.
단일 가이드 폴리뉴클레오티드의 cr뉴클레오티드와 tracr뉴클레오티드를 연결하는 뉴클레오티드 서열은 RNA 서열, DNA 서열, 또는 RNA-DNA 조합 서열을 포함할 수 있다. 일 양태에서, 단일 가이드 폴리뉴클레오티드의 cr뉴클레오티드와 tracr뉴클레오티드를 연결하는 뉴클레오티드 서열은 적어도 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 또는 100개의 뉴클레오티드 길이일 수 있다. 다른 양태에서, 단일 가이드 폴리뉴클레오티드의 cr뉴클레오티드와 tracr뉴클레오티드를 연결하는 뉴클레오티드 서열은 테트라루프 서열, 예컨대, GAAA 테트라루프 서열을 포함할 수 있지만, 이에 한정되는 것은 아니다.
가이드 폴리뉴클레오티드, VT 도메인 및/또는 CER 도메인의 뉴클레오티드 서열 변형은 5' 캡, 3' 폴리아데닐화 테일, 리보스위치 서열, 안정성 조절 서열, dsRNA 듀플렉스를 형성하는 서열, 가이드 폴리뉴클레오티드를 세포내 위치에 표적화하는 변형 또는 서열, 추적을 제공하는 변형 또는 서열, 단백질을 위한 결합 부위를 제공하는 변형 또는 서열, 잠김 핵산(Locked Nucleic Acid, LNA), 5-메틸 dC 뉴클레오티드, 2,6-디아미노퓨린 뉴클레오티드, 2'-플루오로 A 뉴클레오티드, 2'-플루오로 U 뉴클레오티드; 2'-O-메틸 RNA 뉴클레오티드, 포스포로티오에이트 결합, 콜레스테롤 분자에 대한 연결, 폴리에틸렌 글리콜 분자에 대한 연결, 스페이서 18 분자에 대한 연결, 5'에서 3'으로의 공유 연결, 또는 이들의 임의의 조합으로 이루어진 군으로부터 선택될 수 있지만, 이에 한정되는 것은 아니다. 이러한 변형은 적어도 하나의 추가적인 유익한 특징을 가져올 수 있고, 추가적인 유익한 특징은 변형 또는 조절된 안정성, 세포내 표적화, 추적, 형광 표지, 단백질 또는 단백질 복합체에 대한 결합 부위, 상보적인 표적 서열에 대한 변형된 결합 친화도, 세포 분해에 대한 변형된 저항성, 및 증가된 세포 투과성의 군으로부터 선택된다.
일 양태에서, 가이드 뉴클레오티드 및 Cas 엔도뉴클레아제는 Cas 엔도뉴클레아제가 DNA 표적 부위에 이중가닥 절단을 도입할 수 있게 하는 복합체를 형성할 수 있다.
본 발명의 일 양태에서, 가변 표적화 도메인은 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 또는 30개의 뉴클레오티드 길이이다.
본 발명의 일 양태에서, 가이드 뉴클레오티드는 II형 Cas 엔도뉴클레아제와 복합체를 형성할 수 있는 II형 CRISPR/Cas 시스템의 cRNA(또는 cRNA 단편) 및 tracrRNA(또는 tracrRNA 단편)을 포함하고, 여기서 가이드 뉴클레오티드 Cas 엔도뉴클레아제 복합체는 Cas 엔도뉴클레아제를 식물 게놈 표적 부위로 유도하여, Cas 엔도뉴클레아제가 게놈 표적 부위에 이중가닥 절단을 도입할 수 있게 한다. 가이드 뉴클레오티드는 당업계에 알려진 임의의 방법(예컨대, 이에 한정되는 것은 아니지만 유전자총 또는 국소 처리법)을 이용해 직접 식물 또는 식물 세포에 도입될 수 있다.
일 양태에서, 가이드 뉴클레오티드는 식물 세포에서 가이드 뉴클레오티드를 전사할 수 있는 식물 특이적 프로모터에 작동 가능하게 연결되는 해당 가이드 DNA 서열을 포함하는 재조합 DNA 분자를 도입함으로써 간접적으로 도입될 수 있다. 용어 "해당 가이드 DNA"는, RNA 분자와 동일하지만 RNA 분자의 각각의 "U"가 "T"로 치환된 DNA 분자를 포함한다.
일 양태에서, 가이드 뉴클레오티드는 유전자총을 통해, 또는 식물 U6 중합효소 III 프로모터에 작동 가능하게 연결되는 해당 가이드 DNA를 포함하는 재조합 DNA 구축물의 아그로박테리움 형질전환을 위한 개시된 방법 및 조성물을 이용해 도입된다.
일 양태에서, RNA Cas9 엔도뉴클레아제 복합체를 가이드하는 RNA는 듀플렉스 crRNA-tracrRNA를 포함하는 듀플렉스화된 RNA이다. 듀플렉스화된 crRNA-tracrRNA 대비 가이드 뉴클레오티드를 사용하는 한 가지 장점은 융합된 가이드 뉴클레오티드를 발현시키기 위해 발현 카세트만 제조될 필요가 있다는 것이다.
용어 "표적 부위", "표적 서열", "표적 DNA", "표적 유전자좌", "게놈 표적 부위", "게놈 표적 서열", 및 "게놈 표적 유전자좌"는 본원에서 상호교화적으로 사용되며, Cas 엔도뉴클레아제에 의해 식물 세포 게놈에서 이중가닥 절단이 유도되는 식물 세포의 게놈(엽록체 및 미토콘드리아의 DNA 포함) 내 폴리뉴클레오티드 서열을 지칭한다. 표적 부위는 식물 게놈 내의 내인성 부위일 수 있거나, 또는 대안적으로 표적 부위가 식물에 이종성이어서 게놈에서 자연적으로 발생하지 않을 수 있거나, 또는 표적 부위가 자연적으로 발생하는 경우에 비해 이종 게놈 위치에서 확인될 수 있다.
본원에 사용된 용어 "내인성 표적 서열" 및 "고유 표적 서열"은, 식물의 게놈에 내인성이거나 고유하고 식물의 게놈 내 표적 서열의 내인성 또는 고유 위치에 있는 표적 서열을 지칭하기 위해 본원에서 상호교환적으로 사용된다. 일 양태에서, 표적 부위는 LIG3-4 엔도뉴클레아제(미국 특허 공보 2009-0133152 A1(2009년 5월 21일 공개)) 또는 MS26++ 메가뉴클레아제(2012년 6월 19일 출원된 미국 특허 출원 13/526912)와 같은 이중가닥 절단 유도제에 의해 특이적으로 인식 및/또는 결합되는 DNA 인식 부위 또는 표적 부위와 유사할 수 있다.
"인공 표적 부위" 또는 "인공 표적 서열"은 본원에서 상호교환적으로 사용되며, 식물의 게놈에 도입된 표적 서열을 지칭한다. 이러한 인공 표적 서열은 식물의 게놈 내 내인성 또는 고유 표적 서열과 서열이 동일할 수 있지만, 식물의 게놈에서 상이한 위치(즉, 비내인성 또는 비고유 위치)에 위치할 수 있다.
"변경된 표적 부위", "변경된 표적 서열", "변형된 표적 부위", 및 "변형된 표적 서열"은 본원에서 상호교환적으로 사용되며, 변경되지 않은 표적 서열에 비해 적어도 하나의 변경을 포함하는 본원에 개시된 표적 서열을 지칭한다. 이러한 "변경"은, 예를 들어 (i) 적어도 하나의 뉴클레오티드의 치환, (ii) 적어도 하나의 뉴클레오티드의 결실, (iii) 적어도 하나의 뉴클레오티드의 삽입, 또는 (iv) (i) 내지 (iii)의 임의의 조합을 포함한다.
서열 번호 1~189의 요약을 표 1에 나타내었다.
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하기 실시예는 제한이 아닌 예시로서 제공된다.
실시예
본 발명의 양태를 하기 실시예에서 추가로 정의하며, 달리 명시하지 않는 한, 부 및 백분율은 중량 기준이고 온도는 섭씨 온도이다. 이 실시예들은 본 발명의 양태들을 나타내지만 단지 예시로서 제공된다. 상기 설명 및 이 실시예들로부터, 당업자는 본 발명의 양태의 본질적 특징들을 확인할 수 있으며, 본 발명의 사상 및 범위를 벗어나지 않고 다양한 용도와 조건에 맞게 본 발명을 다양하게 변경하고 수정할 수 있다. 따라서, 본원에 나타내고 설명한 것 외에 다양한 변형이 상기 설명으로부터 당업자에게 명백할 것이다. 이러한 변형도 첨부된 청구범위의 범주 내에 속하는 것으로 의도된다.
실시예 1. 식물 재료 및 배지 조성
현탁 배양물, 미숙 자엽, 성숙 자엽, 분할 종자, 배축, 하배축, 상배축, 및 잎을 포함하여 다양한 조직 또는 외식편 유형이 본 방법에 사용될 수 있다. 대두 형질전환, 조직 배양, 및 재생에 사용된 다양한 배지의 조성을 표 2에 요약하였다. 이 표에서, 배지 M1이 형질전환의 출발 물질인 경우, 이는 현탁 배양물의 개시를 위해 사용된다. 배지 M2 및 M3는 상기 열거된 전범위의 외식편에 대한 아그로박테리움 형질전환에 유용한 일반적인 공동 배양 배지를 나타낸다. 배지 M4는 (적절한 선별제의 존재하에) 선별에 유용하고, M5는 체세포배 성숙에 사용되고, 배지 M6는 T0 묘목을 제조하기 위해 발아에 사용된다.
Figure pct00017
1~5일의 공동 배양 후, 조직을 1주일(재생 기간) 동안 선별 없이 M3 배지에서 배양한 후, 선별로 옮긴다. 선별에 있어서, 안정한 형질전환체의 선별을 위해 항생제 또는 제초제를 M3 배지에 첨가한다. 아그로박테리움에 대한 역선택을 시작하기 위해, 300 mg/l의 Timentin®(칼륨 클라불란산과 혼합된 멸균 티카실린 디소듐, PlantMedia, Dublin, OH, USA)을 또한 첨가하고, 선별제와 Timentin®을 모두 선별 기간(총 8주 이내)에 걸쳐 배지에 유지한다. 선별 배지를 매주 교체한다. 선별 배지에서 6~8주 후, 형질전환된 조직은 탈색된(또는 괴사성) 덜 건강한 백그라운드 조직에 반해 녹색 조직으로 가시화된다. 이들 조직 조각을 추가 4~8주 동안 배양한다.
이후, 녹색의 건강한 체세포배를 100 mg/L의 Timentin®을 함유하는 M5 배지로 옮긴다. M5 배지에서 총 4주의 성숙 후, 성숙한 체세포배를 멸균된 빈 페트리 접시(MicroporeTM 테이프(3M Health Care, St. Paul, MN, USA)로 밀봉됨) 또는 플라스틱 박스(섬유 테이프 없음)에 넣고 4~7일 동안 실온에 둔다.
건조된 배아를 M6 배지에 심고, 26℃에서 60~100 μE/m2/s의 광 강도와 18시간의 광주기로 발아시킨다. 발아 배지에서 4~6주 후, 묘목을 습기 있는 Jiffy-7 피트 펠릿(Jiffy Products Ltd, Shippagan, Canada)에 옮기고, 다음의 조건에서 Percival 인큐베이터에 적응될 때까지 투명 플라스틱 트레이 상자에 넣어 보관한다: 60~100 μE/m2/s, 26℃/24℃의 낮/밤 온도에서 16시간의 광주기. 마지막으로, 경화된 묘목을 습기 있는 SunGro 702가 들어있는 2 갤런의 화분에 심고, 온실에서 씨앗을 맺고 성숙하게 성장시킨다.
실시예 2. 대두의 형질전환
대두에 대해 유전자총에 대한 표준 프로토콜(Finer and McMullen, 1991, In Vitro Cell Dev. Biol. - Plant 27:175-182), 아그로박테리움-매개 형질전환(본원에 전문이 참조로 포함되는 문헌[Jia et al., 2015, Int J. Mol. Sci. 16:18552-18543]; US20170121722), 또는 오크로박트룸-매개 형질전환(본원에 전문이 참조로 포함되는 US20180216123)이 본 발명의 방법과 함께 사용될 수 있다.
실시예 3. WUS 발현을 제어하는 GM-LTP3 프로모터가 대두 형질전환을 향상시킴
발현 카세트 GM-LTP3 PRO::AT-WUS::UBQ14 TERM + GM-UBQ PRO::GM-UBQ INTRON1::TAG-RFP::UBQ3 TERM + GM-SAMS PRO::GM-SAMS INTRON1::GM-HRA::GM-ALS TERM(PHP80730; 서열 번호 112)을 갖는 T-DNA를 함유하는 아그로박테리움 균주 AGL1을 사용하여 Pioneer의 대두 품종 PHY21을 형질전환시켰다. 아그로박테리움 감염이 시작되고 4일 후, 조직을 멸균 배양 배지로 세척하여 과잉의 박테리아를 제거하였다. 9일 후 조직을 체세포배 성숙 배지로 옮기고, 이어 22일 후 유전자이식 체세포배를 건조 준비시켰다. 이 시점에, 잘 형성되고 성숙한 체세포배는 RFP 필터 세트를 구비한 형광 입체 현미경하에서 적색 형광을 발하였다. 발육된 체세포배는 기능적이었고, 온실에서 발아되어 건강한 식물을 생성하였다. 체세포배를 생성하고 발아시켜 식물을 형성하는 이 빠른 방법은 아그로박테리움 감염으로부터 유전자이식 T0 식물을 온실로 옮기는 데 소요되는 일반적인 기간을 4개월(종래의 대두 형질전환의 경우)에서 2개월로 단축시켰다.
실시예 4. WUS 발현을 제어하는 GM-HBSTART3 프로모터가 대두 형질전환 성숙 효율을 향상시킴
형질전환이 어려운 대두 품종에서, 대두의 형질전환 과정 중에 형성되는 유전자이식 체세포배의 대다수가 제대로 성숙하지 못하는 것이 일반적으로 관찰된다. 체세포배가 성숙하지 못하면 궁극적으로 온실에서 생존할 유전자이식 묘목을 형성하기 위한 발아의 빈도가 크게 감소한다. 대두 형질전환 성숙 효율에 미치는 영향을 확인하기 위해, WUS 발현을 유도하는 프로모터(GM-HBSTART3(서열 번호 1), GM-LTP3(서열 번호 124), GM-HBSTART2(서열 번호 108), GM-MATE1(서열 번호 109), GM-NED1(서열 번호 110))를 시험하였다. WUS 발현(PHP81343; 서열 번호 116)을 유도하는 GM-HBSTART3 프로모터(서열 번호 1)는 체세포배 성숙 효율을 크게 향상시켜, TAG-RFP 대조군(PHP80728; 서열 번호 111)에서의 8% 미만의 중앙값으로부터 50% 초과로 성숙 효율을 증가시켰다. 도 1 참조. 이는 유전자이식 T0 식물을 형성하도록 발아할 수 있는 유전자이식 성숙 체세포배의 빈도 증가로 직접 표현된다. 도 3a 및 3b 참조.
다른 프로모터를 사용하여 WUS의 발현을 유도한 결과, GM-HBSTART2(PHP80734; 서열 번호 113), GM-LTP3 (PHP80730; 서열 번호 112), GM-MATE1 (PHP80736; 서열 번호 114), 또는 GM-NED1(PHP81060; 서열 번호 115) 프로모터 각각에 대해 중앙값이 13%, 33%, 13%, 및 15%인 중간의 성숙 효율을 나타냈다. 도 1 참조. TAG-RFP 대조군 처리(PHP80728; 서열 번호 111)에서, 아그로박테리움 감염 후 5~6주차에 체세포배는 작고 발육부전을 보인 반면, GM-LTP3 PRO::WUS(PHP80730; 서열 번호 112) 및 GM-HBSTART3 PRO::WUS(PHP81343; 서열 번호 116) 처리에서의 체세포배는 각각 약 2배 내지 5배 더 컸다. 도 2의 A 내지 F 참조.
실시예 5. 형태형성 유전자의 발현을 제어하는 GM-HBSTART3 또는 GM-LTP3 프로모터가 형질전환을 향상시킴
본원에 개시된 GM-HBSTART3 프로모터(서열 번호 1), GM-LTP3 프로모터(서열 번호 124) 또는 임의의 다른 프로모터를 사용하여 형광 마커를 포함하는 발현 카세트에서 애기장대 LEC1, LEC2, KN1, STM, 또는 LEC1-유사(Kwong et al., (2003) The Plant Cell, Vol. 15, 5-18) 유전자의 발현을 유도하면, 체세포배 형성의 빈도 및 유전자이식T0 식물의 재생을 증가시키는 것으로 확인된다. 아그로박테리움 균주 LBA4404를 사용하여, Pioneer의 대두 품종 PHY21의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 형질전환시킨다. 아그로박테리움 감염이 시작되고 4일 후, 조직을 멸균 배양 배지로 세척하여 과잉의 박테리아를 제거한다. 약 9일 후 조직을 체세포배 성숙 배지로 옮기고, 이어 약 22일 후 유전자이식 체세포배를 건조 준비시킬 것으로 예상된다. 이 시점에, 잘 형성되고 성숙한 체세포배는 적절한 필터 세트를 구비한 형광 입체 현미경하에서 형광을 발한다. 발육된 체세포배는 기능적이고, 온실에서 발아되어 건강한 식물을 생성한다. 체세포배를 생성하고 발아시켜 식물을 형성하는 이 빠른 방법은 아그로박테리움 감염으로부터 유전자이식 T0 식물을 온실로 옮기는 데 소요되는 일반적인 기간을 4개월(종래의 대두 형질전환의 경우)에서 약 2~3개월로 단축시킬 것으로 예상된다.
실시예 6. 아그로박테리움 IPT 유전자의 발현을 제어하기 위한 GM-HBSTART3 또는 GM-LTP3 프로모터의 사용이 직접적인 싹 형성을 자극하고 형질전환을 향상시킴
본원에 개시된 GM-HBSTART3 프로모터(서열 번호 1), GM-LTP3 프로모터(서열 번호 124) 또는 임의의 다른 프로모터를 사용하여 형광 마커를 포함하는 발현 카세트에서 아그로박테리움 IPT 유전자의 발현을 유도하면, 다경(multiple shoot) 형성의 빈도 및 유전자이식T0 식물의 재생을 증가시키는 것으로 확인된다. 아그로박테리움 균주 LBA4404를 사용하여, Pioneer의 대두 품종 PHY21의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 형질전환시킨다. 아그로박테리움 감염이 시작되고 4일 후, 조직을 멸균 배양 배지로 세척하여 과잉의 박테리아를 제거하고, 자경 증식을 촉진시키는 배지로 옮긴다. 9일 후 조직을 싹 발육에 유리한 배지로 옮기고, 이어 22일 후 유전자이식 싹을 발근을 촉진시키는 배지로 옮길 것으로 예상된다. 이 시점에, 초기 묘목은 적절한 필터 세트를 구비한 형광 입체 현미경하에서 형광을 발한다. 기능성 묘목이 빠르게 발육되고, 온실에서 계속 성장하여 건강한 식물을 생성한다. 유전자이식 식물을 직접 형성하는 이 빠른 방법은 아그로박테리움 감염으로부터 유전자이식 T0 식물을 온실로 옮기는 데 소요되는 일반적인 기간을 4개월(종래의 대두 형질전환의 경우)에서 약 2~3개월로 단축시킬 것으로 예상된다.
실시예 7. 애기장대 모노프테로스-델타 유전자의 발현을 제어하는 GM-HBSTART3 또는 GM-LTP3 프로모터가 직접적인 싹 형성을 자극하고 형질전환을 향상시킴
본원에 개시된 GM-HBSTART3 프로모터(서열 번호 1), GM-LTP3 프로모터(서열 번호 124) 또는 임의의 다른 프로모터를 사용하여 형광 마커를 포함하는 발현 카세트에서 애기장대 모노프테로스-델타 유전자의 발현을 유도하면, 다경 형성의 빈도 및 유전자이식T0 식물의 재생을 증가시키는 것으로 확인된다. 아그로박테리움 균주 LBA4404를 사용하여, Pioneer의 대두 품종 PHY21의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 형질전환시킨다. 아그로박테리움 감염이 시작되고 4일 후, 조직을 멸균 배양 배지로 세척하여 과잉의 박테리아를 제거하고, 자경 증식을 촉진시키는 배지로 옮긴다. 9일 후 조직을 싹 발육에 유리한 배지로 옮기고, 이어 22일 후 유전자이식 싹을 발근을 촉진시키는 배지로 옮길 것으로 예상된다. 이 시점에, 초기 묘목은 적절한 필터 세트를 구비한 형광 입체 현미경하에서 형광을 발한다. 기능성 묘목이 빠르게 발육되고, 온실에서 계속 성장하여 건강한 식물을 생성한다. 유전자이식 식물을 직접 형성하는 이 빠른 방법은 아그로박테리움 감염으로부터 유전자이식 T0 식물을 온실로 옮기는 데 소요되는 일반적인 기간을 4개월(종래의 대두 형질전환의 경우)에서 약 2~3개월로 단축시킬 것으로 예상된다.
실시예 8. 성장 강화 유전자의 발현을 제어하는 GM-HBSTART3 또는 GM-LTP3 프로모터가 형질전환을 향상시킴
본원에 개시된 GM-HBSTART3 프로모터(서열 번호 1), GM-LTP3 프로모터(서열 번호 124) 또는 임의의 다른 프로모터를 사용하여 형광 마커를 포함하는 발현 카세트에서 아그로박테리움 AV-6B 유전자, 아그로박테리움 IAA-h 유전자, 아그로박테리움 IAA-m 유전자, 애기장대 SERK 또는 애기장대 AGL15 유전자의 발현을 유도하면, 체세포배 형성의 빈도 및 유전자이식T0 식물의 재생을 증가시키는 것으로 확인된다. 아그로박테리움 균주 LBA4404를 사용하여, Pioneer의 대두 품종 PHY21의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 형질전환시킨다. 아그로박테리움 감염이 시작되고 4일 후, 조직을 멸균 배양 배지로 세척하여 과잉의 박테리아를 제거한다. 9일 후 조직을 체세포배 성숙 배지로 옮기고, 이어 22일 후 유전자이식 체세포배를 건조 준비시킬 것으로 예상된다. 이 시점에, 잘 형성되고 성숙한 체세포배는 적절한 필터 세트를 구비한 형광 입체 현미경하에서 형광을 발한다. 발육된 체세포배는 기능적이고, 온실에서 발아되어 건강한 식물을 생성한다. 체세포배를 생성하고 발아시켜 식물을 형성하는 이 빠른 방법은 아그로박테리움 감염으로부터 유전자이식 T0 식물을 온실로 옮기는 데 소요되는 일반적인 기간을 4개월(종래의 대두 형질전환의 경우)에서 약 2~3개월로 단축시킬 것으로 예상된다.
실시예 9. WUS의 발현을 유도하기 위해 GM-HBSTART3 프로모터와 함께 바이러스 인핸서 요소를 사용한 결과 대두 형질전환이 더 향상됨
본원에 개시된 GM-HBSTART3 프로모터(서열 번호 1), GM-LTP3 프로모터(서열 번호 124) 또는 임의의 다른 프로모터를 단독으로 사용하는 것에 비해, 본원에 개시된 GM-HBSTART3 프로모터(서열 번호 1), GM-LTP3 프로모터(서열 번호 124) 또는 임의의 다른 프로모터에 인접한 35S 인핸서와 같은 바이러스 인핸서 요소를 사용하여 형광 마커를 포함하는 발현 카세트에서 WUS의 발현을 유도하면, 체세포배 형성의 빈도 및 체세포배 성숙의 빈도가 더 증가되어, 유전자이식 T0 식물의 재생이 전반적으로 증가된다. 아그로박테리움 균주 LBA4404를 사용하여, Pioneer의 대두 품종 PHY21의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 형질전환시킨다. 아그로박테리움 감염이 시작되고 4일 후, 조직을 멸균 배양 배지로 세척하여 과잉의 박테리아를 제거한다. 9일 후 조직을 체세포배 성숙 배지로 옮기고, 이어 22일 후 유전자이식 체세포배를 건조 준비시킨다. 이 시점에, 잘 형성되고 성숙한 체세포배는 적절한 필터 세트를 구비한 형광 입체 현미경하에서 형광을 발한다. 발육된 체세포배는 기능적이고, 온실에서 발아되어 건강한 식물을 생성한다. 체세포배를 생성하고 발아시켜 식물을 형성하는 이 빠른 방법은 아그로박테리움 감염으로부터 유전자이식 T0 식물을 온실로 옮기는 데 소요되는 일반적인 기간을 4개월(종래의 대두 형질전환의 경우)에서 약 2~3개월로 단축시킨다.
유사한 방식으로 시험된 다른 인핸서 요소들도 GM-HBSTART3 프로모터(서열 번호 1)을 단독으로 사용한 것에 비해 형질전환이 증가되는 것으로 나타난다. 이러한 인핸서는 내인성 식물 인핸서 요소뿐만 아니라 콜리플라워 모자이크 바이러스 35S와 미라빌리스 모자이크 바이러스 2xMMV와 같은 바이러스 인핸서를 포함한다.
실시예 10. 애기장대 WUS 유전자의 동원체가 대두에서 체세포배 형성을 자극하는 기능을 함
하기 처리들을 비교하였다. 유전자총 형질전환 처리의 경우, 모두 GM-EF1A PRO::GM-EF1A INTRON1::ZS-YELLOW::NOS TERM + GM-SAMS PRO::GM-SAMS INTRON1::GM-ALS::GM-ALS TERM을 갖는 플라스미드 QC318(서열 번호 117)을 포함하였다. 처리는 1) 유전자가 첨가되지 않은 대조군, 2) 애기장대 WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 pVER9662(서열 번호 118), 3) Glycine max WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIGMWUS(서열 번호 119), 4) Medicago truncatula WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIMTWUS(서열 번호 120), 5) Lotus japonica WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBILJWUS(서열 번호 121), 6) Phaseolus vulgaris WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIPVWUS(서열 번호 122), 및 7) 피튜니아 WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIPYWUS(서열 번호 123)를 포함하였다.
미숙 자엽을 종자로부터 단리하고, 2주 동안 사전 배양한 후, 유전자총으로 형질전환시켜, 두 플라스미드(첫 번째는 WUS 동원체 각각에 대한 cDNA 서열의 발현을 유도하는 AT-UBI PRO(pVER9662(서열 번호 118), UBIGMWUS(서열 번호 119), UBIMTWUS(서열 번호 120), UBILJWUS(서열 번호 121), UBIPVWUS(서열 번호 122), 및 UBIPYWUS(서열 번호 123))로 이루어진 발현 카세트, 및 ZS-YELLOW에 대한 발현 카세트(QC318(서열 번호 117))를 포함)의 혼합물을 공동 도입시켰다. 외식편을 2주 동안 배양하였다. 2주째, 형광 구형 체세포배의 수를 계수하고 각각의 처리에 대해 표로 만들었다.
유전자총 형질전환 2주 후, 처리된 대조군 자엽에서 형광 구형 체세포배가 거의 관찰되지 않은 반면, 그 외 모든 처리(AT-UBI 프로모터에 의해 유도된 상이한 쌍떡잎식물 종의 WUS 유전자를 함유)의 경우, 처리된 자엽에서 많은 형광 체세포배가 관찰되었다.
실시예 11. 애기장대 WUS 유전자의 동원체가 BRASSICA 에서 체세포배 형성을 자극하는 기능을 함
Brassica의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 애기장대 WUS 유전자의 동원체로 형질전환시키기 위해 단리한다. 하기 처리들을 비교한다. 유전자총 형질전환 처리의 경우, 모두 GM-EF1A PRO::GM-EF1A INTRON1::ZS-YELLOW::NOS TERM + GM-SAMS PRO::GM-SAMS INTRON1::GM-ALS::GM-ALS TERM을 갖는 플라스미드 QC318(서열 번호 117)을 포함한다. 처리는 1) 유전자가 첨가되지 않은 대조군, 2) 애기장대 WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 pVER9662(서열 번호 118), 3) Glycine max WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIGMWUS(서열 번호 119), 4) Medicago truncatula WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIMTWUS(서열 번호 120), 5) Lotus japonica WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBILJWUS(서열 번호 121), 6) Phaseolus vulgaris WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIPVWUS(서열 번호 122), 및 7) 피튜니아 WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIPYWUS(서열 번호 123)를 포함한다.
Brassica의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 유전자총으로 형질전환시키기 위해 단리하여, 두 플라스미드(첫 번째는 WUS 동원체 각각에 대한 cDNA 서열의 발현을 유도하는 AT-UBI PRO(pVER9662(서열 번호 118), UBIGMWUS(서열 번호 119), UBIMTWUS(서열 번호 120), UBILJWUS(서열 번호 121), UBIPVWUS(서열 번호 122), 및 UBIPYWUS(서열 번호 123))로 이루어진 발현 카세트, 및 ZS-YELLOW에 대한 발현 카세트(QC318(서열 번호 117))를 포함)의 혼합물을 공동 도입시킨다. 외식편을 2주 동안 배양한다. 2주째, 형광 구형 체세포배의 수를 계수하고 각각의 처리에 대해 표로 만든다.
유전자총 형질전환 2주 후, 처리된 대조군 외식편 유형(Brassica의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함)에서 형광 구형 체세포배가 거의 관찰되지 않는 반면, 그 외 모든 처리(AT-UBI 프로모터에 의해 유도된 상이한 쌍떡잎식물 종의 WUS 유전자를 함유)의 경우, 처리된 외식편 유형(Brassica의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함)에서 많은 형광 체세포배가 관찰된다.
실시예 12. 애기장대 WUS 유전자의 동원체가 해바라기에서 체세포배 형성을 자극하는 기능을 함
해바라기의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 애기장대 WUS 유전자의 동원체로 형질전환시키기 위해 단리한다. 하기 처리들을 비교한다. 유전자총 형질전환 처리의 경우, 모두 GM-EF1A PRO::GM-EF1A INTRON1::ZS-YELLOW::NOS TERM + GM-SAMS PRO::GM-SAMS INTRON1::GM-ALS::GM-ALS TERM을 갖는 플라스미드 QC318(서열 번호 117)을 포함한다. 처리는 1) 유전자가 첨가되지 않은 대조군, 2) 애기장대 WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 pVER9662(서열 번호 118), 3) Glycine max WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIGMWUS(서열 번호 119), 4) Medicago truncatula WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIMTWUS(서열 번호 120), 5) Lotus japonica WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBILJWUS(서열 번호 121), 6) Phaseolus vulgaris WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIPVWUS(서열 번호 122), 및 7) 피튜니아 WUS 유전자의 발현을 유도하는 AT-UBI PRO를 갖는 UBIPYWUS(서열 번호 123)를 포함한다.
해바라기의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함한 다양한 외식편 유형을 유전자총으로 형질전환시키기 위해 단리하여, 두 플라스미드(첫 번째는 WUS 동원체 각각에 대한 cDNA 서열의 발현을 유도하는 AT-UBI PRO(pVER9662(서열 번호 118), UBIGMWUS(서열 번호 119), UBIMTWUS(서열 번호 120), UBILJWUS(서열 번호 121), UBIPVWUS(서열 번호 122), 및 UBIPYWUS(서열 번호 123))로 이루어진 발현 카세트, 및 ZS-YELLOW에 대한 발현 카세트(QC318(서열 번호 117))를 포함)의 혼합물을 공동 도입시킨다. 외식편을 2주 동안 배양한다. 2주째, 형광 구형 체세포배의 수를 계수하고 각각의 처리에 대해 표로 만든다.
유전자총 형질전환 2주 후, 처리된 대조군 외식편 유형(해바라기의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함)에서 형광 구형 체세포배가 거의 관찰되지 않는 반면, 그 외 모든 처리(AT-UBI 프로모터에 의해 유도된 상이한 쌍떡잎식물 종의 WUS 유전자를 함유)의 경우, 처리된 외식편 유형(해바라기의 미숙 자엽, 분할 종자, 단리된 배축, 성숙 자엽 마디, 하배축, 상배축, 또는 잎 조직을 포함)에서 많은 형광 체세포배가 관찰된다.
실시예 13. 애기장대 WUS 유전자의 동원체가 BRASSICA 에서 형태형성 성장을 자극함.
12개의 상이한 쌍떡잎식물 종, 2개의 겉씨식물, 및 하나의 외떡잎식물 종의 WUS 유전자에 대해, 스펙티노마이신-함유 배지에서 선별 처리를 하면서 Brassica에서 유전자이식 녹색 싹 반응의 성장을 자극하는 능력을 평가함으로써 그 효능을 시험하였다. WUS 발현 카세트를 함유한 모든 처리에 대해, 구축물에 사용된 WUS 유전자를 제외하고 T-DNA의 구성은 동일하였다. 이 T-DNA 구성은 RB + CAMV35S PRO:: WUS ::OS-T28 TERM + GM-UBQ PRO::GM-UBQ 5UTR::GM-UBQ INTRON1::ZS-YELLOW1 N1::NOS TERM + AT-UBIQ10 PRO::AT-UBIQ10 5UTR::AT-UBIQ10 INTRON1::CTP::SPCN::UBQ14 TERM + LB였다(변수 WUS 유전자를 굵은 이탤릭체로 표시).
Brassica napus의 종자를 50% 클로록스 용액으로 표면 멸균 처리하고, MS 기본 염과 비타민을 함유하는 고체 배지에서 발아시켰다. 묘목을 28℃의 명조건에서 10 내지 14일 동안 성장시키고, 하배축을 자엽에서 분리하였다. 하배축 외식편을 200 mM의 아세토시린곤과 함께 10 ml의 20A 배지(표 3)를 함유한 100 x 25 mm 페트리 플레이트로 옮긴 후, 3~5 mm의 길이로 얇게 잘랐다. 전술한 발현 카세트를 함유한 아그로박테리움 용액(550 nM, 0.50 광학 밀도의 아그로박테리움 균주 LBA4404 THY-) 40 μl를 플레이트에 첨가하고, 하배축/아그로박테리움 혼합물을 함유한 페트리 플레이트를 쉐이커 플랫폼에 놓고 약하게 교반하였다. 10분 동안 약하게 교반한 후, 플레이트를 21℃의 약광 조건으로 옮겨 3일 동안 공동 배양하였다.
공동 배양 후, 아그로박테리움 용액으로부터 하배축 외식편을 꺼내, 멸균 여과지에 가볍게 닦아낸 후 70A 선별 배지(표 3)(10 mg/l의 스펙티노마이신 함유)에 놓고, 광실(26℃ 및 밝은 빛)로 옮겼다. 외식편을 2차의 70A 선별로 옮기기 전에 70A 선별 배지에서 2주 동안 유지하였다(대안적으로, 2차 선별을 위해 20 mg/l의 스펙티노마이신을 함유한 70B 배지(표 3)로 외식편을 옮겼다). 2차 선별 후, 외식편을 2~3주 동안 70C 싹 신장 배지(표 3)로 옮기고 다시 광실에 놓았다. 이어서, 싹을 온실의 토양으로 옮기기 전에 90A 발근 배지(표 3)로 옮겼다.
Figure pct00018
표 4에 나타낸 바와 같이, 상이한 종으로부터의 WUS 유전자는 카놀라에서 유전자이식 녹색 싹의 성장 반응을 자극하였다. 이러한 싹 발육의 자극 및 스펙티노마이신 저항성 싹을 재생하는 능력은 WUS 유전자의 공급원에 따라 가변적이었다. 오이의 경우, 이러한 유전자이식 싹의 자극은 음성 대조군 처리에서 볼 수 있는 수준보다 약간 높았지만, 다른 종으로부터의 WUS 유전자의 경우 Gnetum gnemon(겉씨식물)에 대해 최대 95%의 범위이었고, 포도, 거머리말(외떡잎식물), Kalanchoe, 피튜니아, 사과, 해바라기, 카사바, 및 Gnetum의 WUS 유전자의 경우 70%가 넘는 반응이 관찰되었다.
Figure pct00019
실시예 14. 해바라기 WUS 동원체의 발현을 제어하는 HD-ZIP IV 전사 인자 계열의 프로모터의 사용이 BRASSICA에서 형태형성 성장을 자극함
해바라기(Helianthus annuus)로부터 애기장대 WUS 유전자의 동원체의 발현을 유도하는 애기장대 프로모터에 대해, 스펙티노마이신-함유 배지에서 선별 처리를 하면서 Brassica에서 유전자이식 녹색 싹 반응의 성장을 자극하는 능력을 평가함으로써 그 효능을 시험하였다. 모든 처리에 대해, RB + HD-ZIP IV PRO ::HA-WUS::OS-T28 TERM + GM-UBQ PRO::GM-UBQ 5UTR::GM-UBQ INTRON1::ZS-YELLOW1 N1::NOS TERM + AT-UBIQ10 PRO::AT-UBIQ10 5UTR::AT-UBIQ10 INTRON1::CTP::SPCN::UBQ14 TERM + LB 발현 카세트를 함유하는 T-DNA를 사용하였다(변수 프로모터 HD-ZIP IV PRO를 굵은 이탤릭체로 표시).
시험한 프로모터는 애기장대에서 유래되었고, Protodermal Factor 1(PDF1, 서열 번호 149), Protodermal Factor2(PDF2, 서열 번호 150), Glabrous2(HDG2, 서열 번호 151), Meristem Layer1(ML1, 서열 번호 125), Eceriferum6(CER6, 서열 번호 126), 및 Anthocyanless(ANL1, 서열 번호 152)를 포함하였다. 이들 프로모터를 함유하는 플라스미드에 대한 T-DNA 구성은 표 1에 각각 RV027343, RV027344, RV027340, RV027342, RV027338, 및 RV027337로 표시되어 있고, 이들은 각각 서열 번호 175, 169, 173, 174, 172, 및 171에 해당한다. 이들 프로모터를 양성 대조군으로서의 CaMV35S PRO(T-DNA 정보는 RV021090, 서열 번호 153 참조) 및 음성 대조군으로서의 WUS 발현 카세트가 없는 T-DNA(T-DNA 정보는 RV026532, 서열 번호 170 참조)와 비교하였다.
Brassica 종자의 표면 멸균, 묘목을 생성하기 위한 발아, 하배축 외식편의 제조, 아그로박테리움 균주 LBA4404 THY-를 이용한 형질전환, 조직 배양 및 스펙티노마이신을 이용한 선별은 모두 실시예 13에 기재된 바와 같이 수행되었다.
결과는 0 내지 10의 스케일로 제시되며, 0은 음성 대조군의 반응을 초과하는 반응이 없는 것이고, 10은 CaMV35S PRO로 관찰된 강한 형태형성 반응 자극과 일치한다. 시험된 다양한 프로모터에 대해, PDF1 PRO로 관찰된 반응은 5였고, PDF2 PRO의 경우 반응은 2.3, HDG2 PRO의 경우 반응은 2.2, ML1의 경우 반응은2, CER6 PRO의 경우 반응은 2, ANL1 PRO의 경우 반응은 2였다. CaMV35S 프로모터는 WUS의 발현을 유도할 때 바람직하지 않을 수 있는 강한 항시성 프로모터이다(강한 초기 발현이 (싹 형성을 빠르게 자극하기 위해) T-DNA의 통합 직후에는 유익하지만, 전체 식물에서 강한 발현은 형태학적 이상 및 불임을 초래한다). 시험된 HD-ZIP IV 프로모터는 발육배 및 분열조직의 L1(표피) 층에서 발현되었고, 달리 전체 식물에서 발현되지 않았다. 따라서, 식물의 생장부 및 생식부가 발달함에 따라 WUS 발현이 하향 조절되는 T-DNA 전달 후 싹 형성의 양성 성장 자극이 바람직한 결과이며 달성되었다. 싹 형성의 이러한 빠른 성장 자극은 시험된 모든 HD-ZIP IV 프로모터에 대해 입증되었다.
SEQUENCE LISTING <110> PIONEER HI-BRED INTERNATIONAL, INC. DA SILVA CONCEICAO, ALEXANDRE FINCH, ALLISON M GORDON-KAMM, WILLIAM J KLEIN, THEODORE M LOWE, KEITH S <120> TISSUE-PREFERRED PROMOTERS AND METHODS OF USE <130> 7453-WO-PCT <160> 189 <170> PatentIn version 3.5 <210> 1 <211> 3200 <212> DNA <213> Glycine max <400> 1 agttttttag tagtatttta acgttttttg aaacacctaa cattctttta agatattacc 60 ttctgaattc taatttttta tattttcttt caattttatt attaatatat ttatttattt 120 tctttgttat cttttcttaa aataaattat gattttatta ttttttttat cattttatat 180 ttttcaccta cttcaacaaa ctaatttttt agtttccggt ttaccaacta gcttgcctac 240 ttctagctag tttttttact agttttgaca aaactaacta aagtcaaaaa gttaattagt 300 agttgaatgt tgaaaatata tcttattaaa tcataaaatg gttgataaaa ttatttatta 360 aagtaactga acaatataaa attacatgga catattttta tacaactttt aatttgactt 420 ttttctataa aaagaactat aatatttata atgttagtat taaattaatt ttaaacattt 480 gtagcttttt atttatgaaa tataaaaaaa tctacataaa cttattatat atcaccatca 540 atatcatata taataaaatg gtaaaaatga gaaaattcta tcatatgtaa aaaagttaat 600 ttttttttct taaataaaaa agattaaaaa ggaaaactta taaatgttaa agaacaaaaa 660 agaataaata caaaaagtta taaaaactag tgttttaaaa aatataactt aaaattttta 720 ataacttcta acatttttta aaacgttact taaggtaaca ctttttaata tatcaacttc 780 tattttatta tatatcacta tcaatatcat atataataaa atggtaaaaa tgagaaaact 840 ctattatata taaaaagtta aaaataatga attttttcct tatataaaat agattaaaaa 900 aaattctaat aaatattcaa caacaataac ataataaata taaaaagtta caaaaaaaaa 960 ctaacatttt taaaaatata actttagtgt ttcaaaaatc cttagagttg ttaaataaaa 1020 acttatttac caaattttta aactagtaaa aaaattaaaa actaattaaa atatcttgtt 1080 aaatatattt taaatagtgt ataatttatc atgtaagata atattcttat ttttataata 1140 attactttaa aaatcatatc taaaagattt ttaatatgta atattatata tatatatata 1200 tatatatata tatatatata tatattcata aaacatcatt aaaagtgaca ttaaaaacat 1260 attaaaaatc acattaaagc acattaataa catttaattt caatactaag aactctttca 1320 attcttgaac caattacact tgtcttaatg ggggaaaaaa aaacaaactc tatacttatc 1380 cgatcggaaa aactgcaaaa gagaagaaga aatgaatggg agtgacacgc acaaattaat 1440 ttcaagatac cagcacttag tagcaattta gtagcatttg tttggtagcc gtcattcgtt 1500 gcattgcacc gtcaactcgc aaaaaattta ttagtagttt tatacaccaa aaatatgctg 1560 cgtctctttc tctctcccct ctctatcaat tacacctctt ttgccctcca ttaaatgcaa 1620 ttgcaccctc tcaatacgct ctgcaattac tcttcgctgc cacattttcc tccatttgtt 1680 ccccacttcc ctctcctcct tcaatgccct tgtccctatc tcttcttctt ctcctcactt 1740 tctctctgta acacctcttc atgccatagc ttctttttct tttctttttt accatataag 1800 taaacatttt ttttttcaaa actgtcccaa acccttcctt caaaacggtc atctcttttc 1860 aacccaaaac agatctacac cttcatcttc tactcccact ctcactggta agttaaaaaa 1920 aatcttgttt gtaaaacttc tgtcgcaacc agggtcactg cgtgaatctc tgtaacattc 1980 atgttttttt tgttaaggtt gtctaaggtc attgtttttt tttaaagaaa aaaaaaagaa 2040 actaactaat gaaactgttt ggtacaattt tttttttttt ttttgtgagt tctaacattt 2100 ttcaggtgag tcacttgtag ctagggctgt gagaaaagga acgttattag gtctgcaaga 2160 aaaaaaaaga aaaatatcct gatatatagg atatgcgttg taggattttc tccgagatct 2220 tcgtgggtgt tatattttct ttaaaaaagt tttgattttg ttttgcgaaa agacaagatc 2280 agataagggt tacaatattt ctttgctttt taattcatat attttgatat gatatatgca 2340 tagcgtatat ttgtttcgaa attttgatta agtttttttc tctttaatct tttttttctc 2400 ttggaggtca cgtttgtatc actgagtttt tttgtagtaa cacacacact tactcgcttt 2460 gacattgcta tctgacagaa aatccaaggg ctcaacttct taagcttttt ctctatccaa 2520 gaacctctta ggagagagaa agtgatatat atagagagag aaagagtcaa ttctacatat 2580 acttgttggt ggtggttttt gaggcatgtg aagtatgcaa gccgttatta tgatgattcc 2640 ggcgagaaac atgccttcga tgatagcaag gaacaacaac aacgttggag ggttttttgg 2700 atcatcttca tcacctttca ctcttgccca ggtcggtgct tttaactttt caccttctct 2760 ttcaactctt ttgactctct taccttctct tttttttttt tttcacaatt gtccacctct 2820 ttaatttttc accctttttt cattaaaatt tttgaaacaa aattgaccca tctcaaaatt 2880 ttcacttttt taacgatcag tcgatcacat ttgcatcaca aattaaacca accagaccaa 2940 atatccttct cgatattaca aatccgcgtt tccccaaaac tgcgttattt ttctccataa 3000 aaaagtcaaa agaaaaaaaa aattaaactt tctttaaaaa aaacaagaaa gaaagaaaag 3060 aaaatgttga tatgtttgtt gtgtcttttt ctctgagaga gaaccgtact atactacatg 3120 tgtttgaatt gtgtttttgt tagattgaga aactataatc ctctctccgg aaccttaaaa 3180 gatccgtagc aataatcata 3200 <210> 2 <211> 1547 <212> DNA <213> Glycine max <400> 2 agttttttag tagtatttta acgttttttg aaacacctaa cattctttta agatattacc 60 ttctgaattc taatttttta tattttcttt caattttatt attaatatat ttatttattt 120 tctttgttat cttttcttaa aataaattat gattttatta ttttttttat cattttatat 180 ttttcaccta cttcaacaaa ctaatttttt agtttccggt ttaccaacta gcttgcctac 240 ttctagctag tttttttact agttttgaca aaactaacta aagtcaaaaa gttaattagt 300 agttgaatgt tgaaaatata tcttattaaa tcataaaatg gttgataaaa ttatttatta 360 aagtaactga acaatataaa attacatgga catattttta tacaactttt aatttgactt 420 ttttctataa aaagaactat aatatttata atgttagtat taaattaatt ttaaacattt 480 gtagcttttt atttatgaaa tataaaaaaa tctacataaa cttattatat atcaccatca 540 atatcatata taataaaatg gtaaaaatga gaaaattcta tcatatgtaa aaaagttaat 600 ttttttttct taaataaaaa agattaaaaa ggaaaactta taaatgttaa agaacaaaaa 660 agaataaata caaaaagtta taaaaactag tgttttaaaa aatataactt aaaattttta 720 ataacttcta acatttttta aaacgttact taaggtaaca ctttttaata tatcaacttc 780 tattttatta tatatcacta tcaatatcat atataataaa atggtaaaaa tgagaaaact 840 ctattatata taaaaagtta aaaataatga attttttcct tatataaaat agattaaaaa 900 aaattctaat aaatattcaa caacaataac ataataaata taaaaagtta caaaaaaaaa 960 ctaacatttt taaaaatata actttagtgt ttcaaaaatc cttagagttg ttaaataaaa 1020 acttatttac caaattttta aactagtaaa aaaattaaaa actaattaaa atatcttgtt 1080 aaatatattt taaatagtgt ataatttatc atgtaagata atattcttat ttttataata 1140 attactttaa aaatcatatc taaaagattt ttaatatgta atattatata tatatatata 1200 tatatatata tatatatata tatattcata aaacatcatt aaaagtgaca ttaaaaacat 1260 attaaaaatc acattaaagc acattaataa catttaattt caatactaag aactctttca 1320 attcttgaac caattacact tgtcttaatg ggggaaaaaa aaacaaactc tatacttatc 1380 cgatcggaaa aactgcaaaa gagaagaaga aatgaatggg agtgacacgc acaaattaat 1440 ttcaagatac cagcacttag tagcaattta gtagcatttg tttggtagcc attgagaaac 1500 tataatcctc tctccggaac cttaaaagat ccgtagcaat aatcata 1547 <210> 3 <211> 3300 <212> DNA <213> Arabidopsis thaliana <400> 3 taacaatgat aagctacatt gagtgtatca gttctaaaac tttatagtat gctacaatca 60 atttttctaa gtaacaactt caagcaagga atcacacaca cacagtggta cataataaac 120 ttgattttaa tatcatatga tcagcatcat taacggaata agttaagtaa ttcgtcatcc 180 atactactaa gtcatattaa aatcataatc aaacttaaaa gccgattaga aagagagcaa 240 atatatctaa aaattcacga ggaagacgac aaatgcaagg aaacacagct agtattatta 300 aacttaatag atattggatg aatgactgca taatatatat cacattaaaa gtggacataa 360 atttgcatat gtgtaatgta cctctccaca attaatcgcg gaccatttat tttactatta 420 caagtcaagt aactttatat tgttgatcca taattctttt cgaacataaa atcatatact 480 taggccattt tcaactgtca aaactcgaat ccgagaacca aatttcacca ttttccaaaa 540 atgatgagtg tcgaccaaat ggggtactac tgtctaatca ggaacttgtg aacaaatttt 600 caaccttttc caaataagac gagtgtcaac caactttttc caaccaagag atattgggtt 660 gctacacaaa tacttaatag ccattgcata tttatgcata tgcaaatgca gggtcgtggc 720 gtcagaaaga aacataggac cctcaacata tttaatattt tgggagctat atttgactat 780 ttcatattag aaaataataa taaaaaagtg ttggttttat atcaaattgt aatttacgaa 840 aaacttatgc ttttgcgcaa tgatttttgt aaagtatcta ctatgtttag tgtttacatt 900 gattagtagg ctgccgtttt ttttcttgtg tattatgtac tatatatgaa tatgaacatt 960 tgtaaaagtg aatcttgtca ttttcttgtt gaaaacatat atagtatgtg caaacaaagc 1020 ataggttaat ccaataccac acaaataaca cgtcaggtaa atccaataat aaatcgtatg 1080 tgcatgtatg tgtattcatg tatgttacat gaatgtctga atcagtcagt gtacgtatat 1140 gatgtaggtg atgtaaatct taatgtatga gctgtttctt ggaccatggt ccacaatgga 1200 tattgctccc caactacatt agtcaatcga ctggccaatt tttaattaag ataattaatc 1260 caaactacca ttaaatataa ctttgacctt ttttctattc atttttagat attattggaa 1320 cttacgtagt ttacatgcat ctcatccctt tcttttgctc cttgaaagtg ggtccaatca 1380 caaaaaatga tcttatattt tgtattttgt attttaaaaa ctcataatta tataggttca 1440 aaaatttaat taacatcagt gtatactata attactactc tagccaacaa gataaattca 1500 ttttgacatc agccaaaaga taaaaatttg gttaaaaact attggattag cttttagtat 1560 ttaatatttt atgtactgat taaatacgaa tttagaaatc taggatataa gtgagggtgt 1620 ataataaggg aggggtggac cattaatagc gatgtgcaat taaaaattat gattaagaat 1680 ctaggaaatt tgtagattgc ttagttattt ttatggcgat cgtcgtgtca atgtcatgga 1740 ttttgaaact ttaaattaat ctcttaaatt agcacctacc tttgaatttt atagaatctt 1800 tttattttat atgtttaatt ttatagaatc taactagctt attttgagat taaattgttt 1860 agttactttt ataacagtat aaatgtataa tgaggaccta agaatgtagt cctgtaatgt 1920 tcttgctatt ctacttaatc tcatcaccaa tcaaccatca aaagaagcta gtactaataa 1980 aacctgcagg tattcgaata ataattaagc tcaaacacta tactaattta tggaggatta 2040 tatattcaat gaattaggaa cctcatgatg gacattattg actgatataa tgtgtatact 2100 aattgtgagt atttaaaaac catacaaagc atttatatgt ccacatatat tggacacaca 2160 tgcaatcaat gttcaatatg ctccacacac agaaataaaa atactctttc tgatcatatg 2220 atacatcata catatactaa aaaaatctaa aatgaactat aaccacaagc atatataata 2280 acaatgaaat ggtaatgttt cttcattttt atttgttcaa attcttattc ggttgttttt 2340 tcttacccta cgagaatccg tgaggtcaaa gggaaacagt gatttttttt ttgtattttg 2400 ttttttaaat tgatgaactg taaaactctc tctctagaaa aatatataag tagtagtatg 2460 aattttctct cactaaaagc attaatggac ctttcgataa tcataaatgc aatgcaccct 2520 ctctatgcat ttcgcaataa ctccttttcc ttctgccaca tcctcttcct cacctctttc 2580 tcttcttccc tttctcctaa gttcctcctc caccaaattc tccatttatt tcgttaacta 2640 tcctccattt gttttcttct gaagagtgat atattctacc tttctctggt taaagaaact 2700 ccctgaatcc accggttatg tcttgaccgg ctataagcct ataaactgat gccctaagac 2760 acctttttag gtttctcaat aattctccgc atctatcttt tcttctccac aagtaagaga 2820 accagaaaac cagagaagaa gccgagctag ctagggtttc attgtgtgca caaaagtaag 2880 atctctctct ctaaccaata cttgtgtaat ttgtctttgt ttctttgagc aaatattgca 2940 tgtttgttca tattagccgg atccgtttta tattttttca tgatctacat tttatcttta 3000 ttttgtttgt aaattaatga gttttttttt tttttttctg tttttgtcac gatctaaaaa 3060 acaagcgtta caaagaagaa gaaaaacctt tttggagtta gaagtgtaaa aggggtttca 3120 gtttgacgaa ttttccttag tagttgtgta aaaaaaggcc attgacttaa tgtcaactct 3180 atatatctac acattttttt attaattagt ttttgttttt ttcccacttc atttaccttt 3240 agtcaatgaa tttttactga aaacgttttt tcaaggtcaa tttcactgag ttaaaaaaaa 3300 <210> 4 <211> 6022 <212> DNA <213> Glycine max <400> 4 tcgttgaaaa cagaaaatgg tgatgagaat gtatttggtt ggatttttga aaacattttc 60 agtgaaaatg aaaacagaaa ataaccagaa aatgaaaata ataaaatttc gttttcagcg 120 ttttcaattg aaaacaagaa cctcattttg gataaaatga aaatgagatg aaaataaatg 180 taattttaag caaatttaaa aatacaaaaa aagtcaatat atcataaatt ttcaatattt 240 ttatttccta aaaacagaaa gcaaaaagtt aaaccaaaca tatttttaga attctaatct 300 ttagaaaata aaaacaattt tcaaaaaatg aaaataaaaa ataaaaatat aaaccaaaca 360 cacaatattt cagcttgtga gtgcaaaaca tatgcacgtt tttcatttaa gtatttctac 420 agataactag acgaattgaa agttgttata aattttactt caacaaccac cttaaaaggg 480 acctccgcat gatgatgaac gccactagtt agaccatctc taatctctct gcattcttgc 540 atgataagag aataatgaat aattctaaca taaaattagc ttacgacatt ttcgcctcta 600 ttactaattt gctagtattt tatctttatc tacctagctt gcattctgca tatgaaattt 660 gtggcatgta attttattaa tgctatttaa accaatgaat atattgaagc tgcttctatg 720 aattttactt tttaacctaa catcaaatta atgacaaaga aaaggttcaa aaaattattt 780 tgaagcacta ctcgatcaga aaatggttca atgaaatata aatgcatagc tacagtggtg 840 aactaagatc tataatctct atatatcctt atatattctt tcattgatat tcctcttaaa 900 agtacatcca attgattgaa gccttatctt tacatgtata ataaataata aaaaattaga 960 gcaataaaca tatttacaga ttaactgact taattaaaga caataaatat actaaacaaa 1020 aagaataaga ctataagagc atgtctcatt attattaaag gtaattttga aaataatata 1080 taaatttagg ataaattata tatataaaaa ggaacaaaag gcagcggttg attattaaat 1140 tagttaattt gttgatgact tgtaaatcat gagaaactaa agaaaaataa aaaaacacct 1200 caaacttcca ccaaatcacc atatccaccg acagccaaat caagtcataa aatgtcgatg 1260 aacctcccca tctaccttga tcatgagttt gggattcaat tgtagtgcat tgacacctcg 1320 ctttcaaagc accctccagc ctaatttgta gagccagtca aacttgattc tctactttta 1380 cctttattta aaaaatcgtg cttaatatat aatgcaagtt tatagtatac ccattccctt 1440 tcttcacaaa aaaaaaaaat gaggaaatag aacattaagt actaggaata gacagagaag 1500 tctttcttaa gttgggactg ggatctcccg atctggtggg cggtactgta gcttaataaa 1560 tctgactggg gctattaggt ggtgttttgt ttgggagttt atttttttat tttttatttt 1620 taaattttta agatttataa gatatgtatc aataataaat tactttaagt ttaagatatc 1680 atgatatttt ttgtttttca gaaaaggcta aaaatattga ttttttgttt tcagttttgg 1740 atctattttt aaaagaaaat atttttaaaa ttttaaaaaa atacattttt attcttattt 1800 tttttatttg aaaataaaaa caaaaaaaca ctcaaatcac gcatcattct agattttgag 1860 tgtctttttt tctcaatgac aagagactat taatattttt tagttcactt ggtcataaac 1920 taattccact attagatttt gagtgtgtgt taataaaaaa tatttattaa tcaacaattt 1980 ttttatcaac aaatattaat tttattaata gagagaatca aattcacaat tttttttaat 2040 cattcaaccc acctcatatc tcccaaatct atcaccagat taaatctttc taccatataa 2100 gaatcaaatt taaataaaca caattattat ttagtgacta attttgtctt ctaaaattag 2160 tatataagaa aatttcatat ttaacagctc caataagtct cactatgatt atataccaaa 2220 attaaactct tagaagaatt gttactgaaa tcattaatat atatttttat ggagaaacca 2280 ttaaatttca aaagagtctt tctattataa attataattt ataaatatgc ttaatcaata 2340 tttcgcacta ataaataata gaatgtttgc ttataattaa tttaatttgc gacgctaggg 2400 ttgagaaata caggagttaa aagaagttgt tgagttcagt ggacctaggt cacaaaataa 2460 aagttaaatt aagcatgaat gatatcgcgc tctaactctc ttctgttcac gtatatccat 2520 ttacaccagt cgtccctcca taaatgcttc tacaccctct gcaataactc catgctgcca 2580 aaccttctct cacttgttct atttatctcc tcctgcgttg tttcgttctg tctctatttt 2640 gctttggccc aaactaaaac tccatcaact actccttcac ttccctaatt ccccccacac 2700 ccattcttgt ttctaccgtt ccagtcatca tcaacataac taattaaggt ttacaccact 2760 atcaaacgct taaggtcccc tctcgcatat acgcgtgttt aattttagct ttgacttgct 2820 ttgctttgct tagcttagtt aatttctgag tgagagaaag gggtaacggc tacgtccccc 2880 gcattgaaat tggttacttg actagcgtaa tcatctgttt tgtcaaacct ttcacactct 2940 ctacccttat tttgtctttt ctgatttccc tagcttttgt cttttggggt ggcaagtttt 3000 gcgtgtaaga taagagtatt aaggccaacc aaggccaggt aaagtcacca gtgcctcttt 3060 gcaaccttaa cataagaatg aatctccaaa caaacacttg aaagtgctgc atatcgatca 3120 gagacaaaac agcggcacga gctggtcgat gcttttatca gtttcagctt tgcgagacgg 3180 ttccataaat cccaattgca agtaccatca aactttacag agggaaagcg gtcgattcat 3240 tgctgtcatc ttcgtggtta atctaacagg tgatcacttc cctaatttct tattttctct 3300 cctaattaaa cagagataga acaccttttc ttttttgagt tgaacagcaa tttgactaaa 3360 tggtaaaact tgtacatttt ctttattctc gatggggaga tctggcttag aaatttttta 3420 catatagaac tccgattatt aatcgttgtt gttgttatca aatttgttca atttaatgct 3480 ttattgccaa caaattaagt ttagtctttt tgagtaatgt gagtactttt ctaagcatct 3540 aataataagc tattgaagct caatttgcct aaagcaccaa acttaatgct ttattatttt 3600 ttgttttata ggatactttt acttacttta taagaattac aaaataaaat ttagttagtg 3660 attttatatt tgtgaaactt ttatattttt taaatattct gtaaattatt aaaactctat 3720 tatcttatta cagtatttgt ttttatgata aggaagtgga aggaaaatga aaagaaacta 3780 tattattttt ttaatgttta tttgagtggg aaaaaaagtt tcaattctaa ggcccatgag 3840 aaaatataat tccttccctc ccaaatttgg catgaaaggg ttgaggggaa aaatgatctc 3900 ttactcattt ttatttttgt ttttttaata tatatatata tatatatata tatataacat 3960 gattttttat ataaaaaata aatgatttca ccttatgaaa aatagttaaa tataaaaagt 4020 ttgtctttta aagttaattg tatttttttt aattagaatg ataaatataa ctttttttgt 4080 aaggcctgtg gctctttcgt gcttccataa gcatcactat gagactaact taaacagtta 4140 gcccttttcc cctttccccc tttttcaaat atgcaaaccg aactcgtcta tttattttca 4200 cttatctaaa taattacagg aaaaattctc tacgtttttc ttatttccat atatcaaaat 4260 aatacatttt ttctattctt ttaatatttt gttttttatc tttcttattt ttttctctat 4320 tttatttcta attcaaatag aacattaatt tgtttctatc gaatctttgt tacccataat 4380 aaactgtgtg tagtgtatac ttgtaggttt ttttttttta aaaaaaaatc cttctgtctg 4440 ctcaattaac aaataattct aaatcattta atattctctt actaattttc ctacattctt 4500 tatatgtaaa tgttctatat cttcaaacaa aatatcccgg tatacattaa ctaataaccg 4560 aatactctct ctccgtctta agtatcgatc tagattaatt tatccagata aaaaaaaagt 4620 taatacacgt atgaaaaaat aataatttta caaaattaaa tttattaaat aaattaaaaa 4680 aagtactatt ggtattacat taaaaaacta aagtgaaact tactaaaaga gaataatttt 4740 aaatttgact tctggatata taattgtatt aaatagttgt atacataatt tctatccttg 4800 tacaaaattg tctataataa aaaaattcat gtgatttata ccaatttttt cccataccca 4860 tatatattaa tcttgtaaaa tgtttaggat atataattaa tatttttaac tagaactaga 4920 atttgaattt ttacttaagt aaaaaaaaaa ctcattttag aattaaaatc acaattatta 4980 tagcataact aaaatcaacc cttcccaatt atattttgga accaattgac cttaatctca 5040 attcaatttg taaaaaaaaa aagcgtatta aatagaaaaa aatacattaa tattttaaaa 5100 tataagcaat atatttaata catttttcat ttaattaaag aagaagatta gtaagaagat 5160 attgtaagaa aaattgtaat tattgtttgt ttttattttt tttatgcaat agaaataata 5220 gattttatat gattatagaa ataataaatt taaacttaag atttcgtgca aaaatttcaa 5280 attctctcac ctcattacat gaaatatagt atatctttta tttttttcat tttgtgtgca 5340 agttaaagaa cattaattac attaaacaat attactatca ttgttcgtat ttataaaatc 5400 taattaaatt aaaaatacat ctttattcat tactatttcc gttcttattt ataagatcca 5460 aattattaaa aaaacaattt aaatatttaa ttgaatatga ttgtttggta catccttaat 5520 tgaatatgtt taataatttc ttaagaaatg tgtggataat agttaacatg aggcttattt 5580 tctttttatt aatgctaata cttattaaat tatatatttt catatttttg agatgccaga 5640 tgtattggat ttttaatttt taatacattt tacaaaatgt acatagaaag tcaatataaa 5700 taatataatt aatttaaaag aaatagcatt gctcatttac tattttacta aaaatattaa 5760 atttttgcct tatgtagtta tgaacattta atgtaataat aaactaattc taatgtatat 5820 tatattgatt attgaacttc tttaaaatat attcttaagg gccctcgcta tatagggcta 5880 cacaaactac actcttcttg gtcgatctcc cacatttttc tctacccctc gcgcgtgtta 5940 aattttttga aatcctggtt ttgcagagaa gctatagaga agaagattgc ggcttgtgaa 6000 cttaaggatt taagtgaagt ca 6022 <210> 5 <211> 5966 <212> DNA <213> Glycine max <400> 5 tcgttgaaaa cagaaaatgg tgatgagaat gtatttggtt ggatttttga aaacattttc 60 agtgaaaatg aaaacagaaa ataaccagaa aatgaaaata ataaaatttc gttttcagcg 120 ttttcaattg aaaacaagaa cctcattttg gataaaatga aaatgagatg aaaataaatg 180 taattttaag caaatttaaa aatacaaaaa aagtcaatat atcataaatt ttcaatattt 240 ttatttccta aaaacagaaa gcaaaaagtt aaaccaaaca tatttttaga attctaatct 300 ttagaaaata aaaacaattt tcaaaaaatg aaaataaaaa ataaaaatat aaaccaaaca 360 cacaatattt cagcttgtga gtgcaaaaca tatgcacgtt tttcatttaa gtatttctac 420 agataactag acgaattgaa agttgttata aattttactt caacaaccac cttaaaaggg 480 acctccgcat gatgatgaac gccactagtt agaccatctc taatctctct gcattcttgc 540 atgataagag aataatgaat aattctaaca taaaattagc ttacgacatt ttcgcctcta 600 ttactaattt gctagtattt tatctttatc tacctagctt gcattctgca tatgaaattt 660 gtggcatgta attttattaa tgctatttaa accaatgaat atattgaagc tgcttctatg 720 aattttactt tttaacctaa catcaaatta atgacaaaga aaaggttcaa aaaattattt 780 tgaagcacta ctcgatcaga aaatggttca atgaaatata aatgcatagc tacagtggtg 840 aactaagatc tataatctct atatatcctt atatattctt tcattgatat tcctcttaaa 900 agtacatcca attgattgaa gccttatctt tacatgtata ataaataata aaaaattaga 960 gcaataaaca tatttacaga ttaactgact taattaaaga caataaatat actaaacaaa 1020 aagaataaga ctataagagc atgtctcatt attattaaag gtaattttga aaataatata 1080 taaatttagg ataaattata tatataaaaa ggaacaaaag gcagcggttg attattaaat 1140 tagttaattt gttgatgact tgtaaatcat gagaaactaa agaaaaataa aaaaacacct 1200 caaacttcca ccaaatcacc atatccaccg acagccaaat caagtcataa aatgtcgatg 1260 aacctcccca tctaccttga tcatgagttt gggattcaat tgtagtgcat tgacacctcg 1320 ctttcaaagc accctccagc ctaatttgta gagccagtca aacttgattc tctactttta 1380 cctttattta aaaaatcgtg cttaatatat aatgcaagtt tatagtatac ccattccctt 1440 tcttcacaaa aaaaaaaaat gaggaaatag aacattaagt actaggaata gacagagaag 1500 tctttcttaa gttgggactg ggatctcccg atctggtggg cggtactgta gcttaataaa 1560 tctgactggg gctattaggt ggtgttttgt ttgggagttt atttttttat tttttatttt 1620 taaattttta agatttataa gatatgtatc aataataaat tactttaagt ttaagatatc 1680 atgatatttt ttgtttttca gaaaaggcta aaaatattga ttttttgttt tcagttttgg 1740 atctattttt aaaagaaaat atttttaaaa ttttaaaaaa atacattttt attcttattt 1800 tttttatttg aaaataaaaa caaaaaaaca ctcaaatcac gcatcattct agattttgag 1860 tgtctttttt tctcaatgac aagagactat taatattttt tagttcactt ggtcataaac 1920 taattccact attagatttt gagtgtgtgt taataaaaaa tatttattaa tcaacaattt 1980 ttttatcaac aaatattaat tttattaata gagagaatca aattcacaat tttttttaat 2040 cattcaaccc acctcatatc tcccaaatct atcaccagat taaatctttc taccatataa 2100 gaatcaaatt taaataaaca caattattat ttagtgacta attttgtctt ctaaaattag 2160 tatataagaa aatttcatat ttaacagctc caataagtct cactatgatt atataccaaa 2220 attaaactct tagaagaatt gttactgaaa tcattaatat atatttttat ggagaaacca 2280 ttaaatttca aaagagtctt tctattataa attataattt ataaatatgc ttaatcaata 2340 tttcgcacta ataaataata gaatgtttgc ttataattaa tttaatttgc gacgctaggg 2400 ttgagaaata caggagttaa aagaagttgt tgagttcagt ggacctaggt cacaaaataa 2460 aagttaaatt aagcatgaat gatatcgcgc tctaactctc ttctgttcac gtatatccat 2520 ttacaccagt cgtccctcca taaatgcttc tacaccctct gcaataactc catgctgcca 2580 aaccttctct cacttgttct atttatctcc tcctgcgttg tttcgttctg tctctatttt 2640 gctttggccc aaactaaaac tccatcaact actccttcac ttccctaatt ccccccacac 2700 ccattcttgt ttctaccgtt ccagtcatca tcaacataac taattaaggt ttacaccact 2760 atcaaacgct taaggtcccc tctcgcatat acgcgtgttt aattttagct ttgacttgct 2820 ttgctttgct tagcttagtt aatttctgag tgagagaaag gggtaacggc tacgtccccc 2880 gcattgaaat tggttacttg actagcgtaa tcatctgttt tgtcaaacct ttcacactct 2940 ctacccttat tttgtctttt ctgatttccc tagcttttgt cttttggggt ggcaagtttt 3000 gcgtgtaaga taagagtatt aaggccaacc aaggccaggt aaagtcacca gtgcctcttt 3060 gcaaccttaa cataagaatg aatctccaaa caaacacttg aaagtgctgc atatcgatca 3120 gagacaaaac agcggcacga gctggtcgat gcttttatca gtttcagctt tgcgagacgg 3180 ttccataaat cccaattgca agtaccatca aactttacag agggaaagcg gtcgattcat 3240 tgctgtcatc ttcgtggtta atctaacagg tgatcacttc cctaatttct tattttctct 3300 cctaattaaa cagagataga acaccttttc ttttttgagt tgaacagcaa tttgactaaa 3360 tggtaaaact tgtacatttt ctttattctc gatggggaga tctggcttag aaatttttta 3420 catatagaac tccgattatt aatcgttgtt gttgttatca aatttgttca atttaatgct 3480 ttattgccaa caaattaagt ttagtctttt tgagtaatgt gagtactttt ctaagcatct 3540 aataataagc tattgaagct caatttgcct aaagcaccaa acttaatgct ttattatttt 3600 ttgttttata ggatactttt acttacttta taagaattac aaaataaaat ttagttagtg 3660 attttatatt tgtgaaactt ttatattttt taaatattct gtaaattatt aaaactctat 3720 tatcttatta cagtatttgt ttttatgata aggaagtgga aggaaaatga aaagaaacta 3780 tattattttt ttaatgttta tttgagtggg aaaaaaagtt tcaattctaa ggcccatgag 3840 aaaatataat tccttccctc ccaaatttgg catgaaaggg ttgaggggaa aaatgatctc 3900 ttactcattt ttatttttgt ttttttaata tatatatata tatatatata tatataacat 3960 gattttttat ataaaaaata aatgatttca ccttatgaaa aatagttaaa tataaaaagt 4020 ttgtctttta aagttaattg tatttttttt aattagaatg ataaatataa ctttttttgt 4080 aaggcctgtg gctctttcgt gcttccataa gcatcactat gagactaact taaacagtta 4140 gcccttttcc cctttccccc tttttcaaat atgcaaaccg aactcgtcta tttattttca 4200 cttatctaaa taattacagg aaaaattctc tacgtttttc ttatttccat atatcaaaat 4260 aatacatttt ttctattctt ttaatatttt gttttttatc tttcttattt ttttctctat 4320 tttatttcta attcaaatag aacattaatt tgtttctatc gaatctttgt tacccataat 4380 aaactgtgtg tagtgtatac ttgtaggttt ttttttttta aaaaaaaatc cttctgtctg 4440 ctcaattaac aaataattct aaatcattta atattctctt actaattttc ctacattctt 4500 tatatgtaaa tgttctatat cttcaaacaa aatatcccgg tatacattaa ctaataaccg 4560 aatactctct ctccgtctta agtatcgatc tagattaatt tatccagata aaaaaaaagt 4620 taatacacgt atgaaaaaat aataatttta caaaattaaa tttattaaat aaattaaaaa 4680 aagtactatt ggtattacat taaaaaacta aagtgaaact tactaaaaga gaataatttt 4740 aaatttgact tctggatata taattgtatt aaatagttgt atacataatt tctatccttg 4800 tacaaaattg tctataataa aaaaattcat gtgatttata ccaatttttt cccataccca 4860 tatatattaa tcttgtaaaa tgtttaggat atataattaa tatttttaac tagaactaga 4920 atttgaattt ttacttaagt aaaaaaaaaa ctcattttag aattaaaatc acaattatta 4980 tagcataact aaaatcaacc cttcccaatt atattttgga accaattgac cttaatctca 5040 attcaatttg taaaaaaaaa aagcgtatta aatagaaaaa aatacattaa tattttaaaa 5100 tataagcaat atatttaata catttttcat ttaattaaag aagaagatta gtaagaagat 5160 attgtaagaa aaattgtaat tattgtttgt ttttattttt tttatgcaat agaaataata 5220 gattttatat gattatagaa ataataaatt taaacttaag atttcgtgca aaaatttcaa 5280 attctctcac ctcattacat gaaatatagt atatctttta tttttttcat tttgtgtgca 5340 agttaaagaa cattaattac attaaacaat attactatca ttgttcgtat ttataaaatc 5400 taattaaatt aaaaatacat ctttattcat tactatttcc gttcttattt ataagatcca 5460 aattattaaa aaaacaattt aaatatttaa ttgaatatga ttgtttggta catccttaat 5520 tgaatatgtt taataatttc ttaagaaatg tgtggataat agttaacatg aggcttattt 5580 tctttttatt aatgctaata cttattaaat tatatatttt catatttttg agatgccaga 5640 tgtattggat ttttaatttt taatacattt tacaaaatgt acatagaaag tcaatataaa 5700 taatataatt aatttaaaag aaatagcatt gctcatttac tattttacta aaaatattaa 5760 atttttgcct tatgtagtta tgaacattta atgtaataat aaactaattc taatgtatat 5820 tatattgatt attgaacttc tttaaaatat attcttaagg gccctcgcta tatagggcta 5880 cacaaactac actcttcttg gtcgatctcc cacatttttc tctacccctc gcgcgtgtta 5940 aattttttga aatcctggtt ttgcag 5966 <210> 6 <211> 456 <212> DNA <213> Zea mays <400> 6 aagcttgctt tcacatctcc tccggtcctc ctccatgtgt acaccaaaca aaccaagaag 60 aaaaaaatgc atggttttgt tcccgattta taccttggag caacccttcc attctcttga 120 aattttcttt gtcgcccctt gaacttcccc ccatatgtcc agtccagcta caaattaatg 180 gcgacgagcg atgaaacgca cggaacatat atgcctcagc aggcaaacgt cccagtcgtc 240 gatctctggc acttaattaa ttgtttatat taattccaac tcgttcgtgc aacggtgcaa 300 acaaaacaag ctgacgatgt cgagtcgctg tttccccttc caccttaatt gcttttgcag 360 ccaaacctcc tgctggacaa ccagcagctc cagctactgc agcagcaaca cctgctggac 420 cagatcccgg cgacgacggc ggagagcggc gacaac 456 <210> 7 <211> 3000 <212> DNA <213> Oryza sativa <400> 7 ttaaaaaaat ctggaaaaaa gaagcgattt aaaaaaggaa acaaaaaagt gacgttaaaa 60 taggagatct taaaaaaaag aaacgcgtag ggatgcagct ggtgcgcgcg ccggctcctt 120 tcccttgcgc gcacgcgcgc tagcatcccc cataagttta acccataaat tatatttgct 180 tcgttcgttt ctcaatggct tgttagtcat aggtcaatca atcagagtct agaaaaagag 240 gtaatttttt ccattgttga gaagatatta ggagatacta tttttttagt gtgaaatttg 300 atatctatac tagctatctc ggtataaaaa ttactttaaa tgtaaaattt agatgcctca 360 atatatatac ttttttaaga accgtataat ttctcataga aaaaagcaat gaagacggac 420 atgactctca ttagtagtgt actttttttt ttcctaatcc actatatagt gtaccattat 480 ccattaattt cttttatctt ttacaacttt gattttgagg gatttgcata ctgttttgaa 540 aggttgattg tggttatttc tcacttgatt agatgcatga gtttttaaaa taatttaatt 600 tcaagtagat acaatactca tgtatagatg atataattgt gtattattcc ctccatccta 660 cgtattttta gtgtttaaaa tttgttagca tttcttgagt attattttcc taacccacct 720 aattaaccac tgaaattatt tctcactagt ttgcatcgct gggcatgata tttcttatta 780 aggatgtgtt tagttcacat taaaattgga agtttggttg aaattgaaac gatgtgacgg 840 aaaaattaaa agtttaggtg tgtaggaaag ttttgatgtg atagaaaagt tggaagtttg 900 aagaattatt ttgaaactaa acacggcata aagtgaactc tcgggaggcc gggggcatcg 960 tgaaataaaa aagaaatgtt ggatatcaga gaaggggctc tcgtattgaa attttgttaa 1020 gaggggtgag tttcgaatcg ggatcggcga gcccatactt taagaggata tcttgaaata 1080 atttcaccgt aaccttaatt tacgctaaaa tccctaaaca tatttatatt cgtggacatg 1140 gcgagtacat gccacgtttc ttctgctagt aatttttcac ataaattttt ttttctcagc 1200 aagaaaggct ggctatatac tccagtgcag aaagagtgaa aaagactaca ccaaacaagc 1260 atgaggcgtg tggaggcatg aaaacagtac gtacccaggg cacagcagtg aaaacatcga 1320 tctcttcacc ctgcatggtg tctccttaaa atctgatcga tcgactccca aaaatataag 1380 taaaatactc gagtcaaaat gcatcagttt gcatgcatag tgaaacagca gcaagctggt 1440 cttccacagg atactttttg acccattcct cctatacatg catgtaatta atcacaaagt 1500 tgcaaaaaat acaatactat gattgtgtat ttgtgtgtac tatatataaa acaaataaat 1560 taacctagta gtaactgatc agtatcgatc gagagtcaac aaaatatatg tagactacga 1620 cgatacgtgt gtgccttcct gttttctttt gttcttatct ttgggccgca ctatacgctg 1680 catctatact aaacagtagt gaaaaatcta cctctccacc actcaattat tacgccggtc 1740 tataataatt cgttgtataa attacggggc aacagtgaat tattcatgtg ctcttgcgtg 1800 taggattgcc aaacaactgt ttcatggtca gataaatgaa aaaaaagcga caaattggta 1860 acaataagga aatgttaatt aatttatgac atgtattact ttcattaaaa atataatagt 1920 ttctgactat acatgtatct cggaggtaca taaccaaaaa ttcttagagg aagtataaaa 1980 aaaatgtttt cttgttattg gctacgaggt ctcccaaaaa tccaatgttg tacaaatact 2040 atattacatg tcaatttgaa attgtgttaa taagtaaaaa aggacactcc aatttctcca 2100 tatcctcaat tgaggaaata taaaaacaaa atttgtttaa aaaaatgttt caggaagtat 2160 aaaaaatgtt ttattgttct tggctatgag gtctcccaaa aatccagtgt tgtacaaaca 2220 ctatattaca tgtcaatttg acattgtgtt aataaataat aagtcaaaaa aggacactct 2280 aatttctcca tatcctcaat tgagggaata taaaaacaaa aattggaatt gaaagttgaa 2340 accatgcacc actagtctct ctctctctct ctctctctct ctctcccctt ccatctcttt 2400 ctctccccta gcaattacac tacgccttct ccataaatgc gattgcaccc tctcaacact 2460 ccgcggcaat aactccttcg cccccctctc cacattctct tccacctgtg ttcccctttt 2520 cttctcctct tcttcttcct ctcctcctcc tgatcctgat cgagaagatc tcaccttgat 2580 ctcttgccgt gtgatctcta gctctcgcta gccctctgaa ccttgcagtt tgttgtttgc 2640 acccggtgaa acatcttctt ttgtgcaggt aggagagaca gcaaagcaga gataagagtt 2700 ctatatttgc atgtagttct tgtcttggat ctcactggct ggctgtctcc tctctgcagc 2760 aaggttcgtt gggctagctt gtgtgctaga tctgtttcta cttgcgtttg cgttgcttgg 2820 caagcaagat atatatatat atatctatat ctatctatct atcattctat ctccctacat 2880 atttagagct agcactttgt gtgttctttg ggtttgggac acactcatat acgcgtgttc 2940 atgagtggaa atggaaggag ctgcaagggc aagaattagg gtttgagata taggagtata 3000 <210> 8 <211> 3000 <212> DNA <213> Arabidopsis thaliana <400> 8 ttctgatcga aaccgagagg ataatttgga ataagtaata acgaataatt tgttaacaag 60 tcgcttttta tagattcctt gtgagaaaac gtctttatca tatcaaacga tttttggtga 120 gttattaatc ttgtcgcagc attaagcata tatgatgcca ctttaggtcg tcggttacgt 180 taattaactt acataaaatt agatccaaga aaaaaaaatc ttgcataaga aaagaagaaa 240 gaactagttt atttattaag ctgtgagttt atcctaaaat atagtttggc ttgtaaacta 300 gaattacata tagcagctgc aaaaaatgat ggtgatgatg attttcacat tgacaacaac 360 aattatacaa cgttatgatg gtcgtcaatc gatatcaact cgaaaatata atttagaact 420 tttgcatata cataggcaca cacatatttt tgtatgtatg aacatggttt agtgtatagt 480 tgatagttta aagacattga ttctttgaat ggtcggagct tttagtgcgt ttggttggtc 540 ggatcgcctt gaaagctaaa aaaagacaac tttgtccgta tgtgttgtcc tagttttgga 600 gtaatttatt ttcgaccgtt ttagccatta gcggaaacca cctaactctc tctatcaaat 660 gttctttagc gttggtccgt tgcaaacatt tgatcactgt attaataaca tcgaccatac 720 gatgctgttt tgttcgtacc aaattcaccg tagtaaacgc tgaaactttt tttatttttt 780 tgtgtgtgtg cgcacaaaac gctgaaacta gttaaaatat aaatatattg gataataagt 840 ataaagtacc caatatattt gtatctattt atattcgtga catttgaatc gagttttaca 900 tggtatcgga gtaacgagac aatcgatacc atattattgc tttgacctac cagttaatca 960 atatatacat atttcaagtc tattaactcg taagtctaat acaattagaa aaatgaaaaa 1020 aaataaaaga agaagatata tatggaaata ttggcatttg tcttaataat gtaatcaatc 1080 atcaattgta gaagccaaag gcatctctag tgtcaacgaa tttgaattga tataccaact 1140 aacatacttt caaaaccggt agattatacg gtattggaag atgatgaaaa gaagagttta 1200 tttctcttca aaagttacaa attatataaa tgtcatatac cgtaagatga gatagttgaa 1260 tattcacgtg gtgtttatag tgtctacata ggtaaaagac attaatcata caatcgtatt 1320 aacaaaattg attgtaaaaa aaaaaaaaaa agggttgatt gtaagttcgt aacctctatg 1380 tggactgtgt ggcccaaacc aatcggtcgg aagctacaat actgtgtccg aatcctaaaa 1440 ggctaaaaac tgactcggac cagcttagag atcatatgaa attatggata gcggaatagc 1500 tggcaacttc aatgaattct gaatttaaaa catttttttt tatatatata gatgatttgg 1560 cgattttcga actggaatgg tgaaaaagtg gttgagtaca ctattagttt atttatttaa 1620 aaaatagagg gaaattcaaa tgaaaaaaaa agtttcatct acagaaaaaa aaacacgtca 1680 aaattccaaa aaacttgaac gtcttgaaca acggtataac cgtataagaa agggaactta 1740 aaacttaaac gtctacatga ttgaaagggt aaaaatattc atattacaat ggtataagaa 1800 aatggagaag ccagaggagc aactccaaag aagaagatgc agcaagagag tgtacattat 1860 tataattata agttggtata tatgtagttg ctaaaaaaaa aaaagagtgt acaatatttt 1920 ctgaagaagc atcgagctgg ttaatattta agacccacca aaacctacca acaatgttat 1980 tctcatctct aagcaaaagt ttgatctcat cttttttttt aatttttttt ttgatgggtc 2040 ctcttctacc tctgcatctc cacgtgctat acaagtggcc tcgctgtatg tattatttac 2100 cactctctta ttgttactaa aaacttgaat gtcacttgaa tgtcacttga atgcaaacta 2160 tatatttagg tgaataccag atcaaatagc aataaataaa ccaacaattt atgctaatta 2220 aaaacttgaa aaaacacttt ccattaccta tgatttttta atccaatctt ggggtttgat 2280 gcccataaaa cgaagtcgaa aagagaaaag cgaccttaat tgtaataaat gcagctattg 2340 acttctctat caacttcaag tcttcaagta actacaaatt taataatctc cttagcttat 2400 cttagagaag cacaaaacaa aagggtctac gcttagtctt cctagattct tgatttgagt 2460 cttgatggcc acttgctttt tatgtctcaa tcctaagagt tgatacttac ttagtattaa 2520 tcaggtttca cattatctca gacctagctt gcatttttga tacaaacatg tcatgcaata 2580 cacataagca acacgtaccg agtcattgat tgaaaaacat gacctaaata gtaatacgga 2640 ctgagagaga gtgatgaatc atttcatatg ggagacagct ttctcatacg cgtccatgcc 2700 tttgcatgaa agattaaata attcaactct ttcacttatc ttattttaat ttactttgca 2760 gaattccaag ttttattgca atcaccaggg gtattaaatg ggatagtgta aatgcacctg 2820 caacacatca actaaccaca cactcctcca cctgtttgta tttacttcaa aaacttaatt 2880 caaaaatccc tttgcttctt tccttccctc tatttataaa ccctagtatc cggtttttat 2940 tctaccacac aataccattt gcaacaaact tctctctgaa tctcagtgaa ttctcataaa 3000 <210> 9 <211> 2546 <212> DNA <213> Glycine max <400> 9 aaaatatatt aaaagcgtga gtttatttgt ttatactttt tgcaatatct atatttttaa 60 taataaatta agatcttcaa ttttattata caatatttca agattaattg aaacattttt 120 tttttcaatt aattcccgtc caaaattttc aattgtctaa ttattatgac tgcttagtct 180 tattaataaa aataataata aatgatgtat catatcatat taagatttat caactataat 240 ttaaattaaa ataagtgttt ttttaaaata aaataaatga ttttataatt tttttgatgc 300 atttgtttaa ttttttattt aaaataagta attttttgtt ttcttaaaac caaccctatc 360 tgcttaatca tttttctaaa ttttttcaaa aaatttaaac aaattagttc ataatatttt 420 atgactttaa ataattcagt accagcaact gcctcattga ctttgcactt gccactgtcc 480 tgcctttgca cgaaaaataa catataaata acgcgtagca ctgttttcta tatctaaatt 540 agtctatata aaacataata attacataaa tattcggaga ttttataaac accgacaaaa 600 aataaagttt aaagtttgtg atttttatgt caatttcttt ttattcataa gaattgaact 660 caaatataaa aaaaatttat aacaacgtac aactaaatta gactcattga tatttttatg 720 tgaaatttaa gtttaaccaa acaatattta tctagatata tgcataaaga aagagttttt 780 tttttagttt atacttagac caatttttat tttatttttt atggaaactt agaacagatg 840 ttaatgagca gaaatatcaa ttatgaaata aaaatacaag taattaatat tttattttag 900 ttcttatagc tcaatttttt aataacactt ttttggtcct tgttctctaa aattatttaa 960 cgtaattaca tttagatctt ttaaaagtgt tacaaaactg caacggatag tgtaaattaa 1020 aaccgctaaa aagagatttg ggtaaatttt aatggtcata aataaataca acaacaacgc 1080 cttatcccac taggtgagtc ggttacatgg atcaacttcc gcctaatcgt cataaataaa 1140 taaagaaaag aaaacaagac tttgttgtgc catcaaccat ggccaaggca gaatcacgcg 1200 tgacaaactt tcataaacga attgagaacc ttattggcat tgggtcagag ccatcggcga 1260 ctgtgcttgt tttgttgacg gatttacatc atcaattcca gttttgtaac aattttgaag 1320 aatatggatg tttgcattag gcaactttag acatccaata ccaaaaggtt taacaattgt 1380 aactatatgg accaaaactc ctattttaaa caataggtat caaaagctaa ttaaactttt 1440 attaaaatag caagcctagg aagcatacgt gtgtatatac taaaattgct gcatgagtga 1500 actacacaaa ttcaatataa ttgttcacca tacttcagac aaaataatgt tggcccagtc 1560 ccatttaatg taagactcaa aatctatgcc agttgacccg aaccacacac aaacaatggt 1620 agaaatttgg agtacgtata ttggccacag tagcctactc aactgaaaag caaccaacct 1680 atgaactttg ctagctgttt ccaagccata gcaattaaac ttttcccctt ttacttttat 1740 tttcttgaaa tttttatgta ccaaggtggg ttggaaattt caaattggta aatcaggtta 1800 attcctttct attagctagg tgcactcaat cttccatgtt tatatacact ataacatttt 1860 atacagcatc accttctgta tgtctattta ttaatacaga aaagcataca catactatgt 1920 attgattgcc acagcctggt acaatcagcc acaactgttt ctcaaagtaa cttgtatatt 1980 gatattcatt tatacctaag ggtatgtctc tatcctgctc ccacaaatgt gggggaactg 2040 tgttgtagat ttcactttca agcgattgat attatatgtt caaggtaact cccctaatta 2100 atatattgaa tgagacaatt tgtgtaatgc taaataaggt ttctatatcc taaacttctt 2160 tagagtaaac atatgctaag aaaatggggt gtaccttcaa ttagtaccaa atttgcacac 2220 aagttgctta agcttaattt tgaaaaaaaa taaaagggtt tggagagaga ggatatttgg 2280 atgtattaga catgtgctca aggttttttc agaaaatttc aggctatatt acaagaagta 2340 gtgaattaat atcatgagta aatgcaccct gcaaaacatc aactaaccac acactcttcc 2400 cacccgctaa tatttacttc tcacttctgt gaactgtttt atattccatg ctttctccct 2460 tccccaaacc acacttcaaa atcctctggt aacccttatt ttatctattc tctctctttc 2520 ttttgtgttc taagcagcta gaagca 2546 <210> 10 <211> 2500 <212> DNA <213> Glycine max <400> 10 aaaatatatt aaaagcgtga gtttatttgt ttatactttt tgcaatatct atatttttaa 60 taataaatta agatcttcaa ttttattata caatatttca agattaattg aaacattttt 120 tttttcaatt aattcccgtc caaaattttc aattgtctaa ttattatgac tgcttagtct 180 tattaataaa aataataata aatgatgtat catatcatat taagatttat caactataat 240 ttaaattaaa ataagtgttt ttttaaaata aaataaatga ttttataatt tttttgatgc 300 atttgtttaa ttttttattt aaaataagta attttttgtt ttcttaaaac caaccctatc 360 tgcttaatca tttttctaaa ttttttcaaa aaatttaaac aaattagttc ataatatttt 420 atgactttaa ataattcagt accagcaact gcctcattga ctttgcactt gccactgtcc 480 tgcctttgca cgaaaaataa catataaata acgcgtagca ctgttttcta tatctaaatt 540 agtctatata aaacataata attacataaa tattcggaga ttttataaac accgacaaaa 600 aataaagttt aaagtttgtg atttttatgt caatttcttt ttattcataa gaattgaact 660 caaatataaa aaaaatttat aacaacgtac aactaaatta gactcattga tatttttatg 720 tgaaatttaa gtttaaccaa acaatattta tctagatata tgcataaaga aagagttttt 780 tttttagttt atacttagac caatttttat tttatttttt atggaaactt agaacagatg 840 ttaatgagca gaaatatcaa ttatgaaata aaaatacaag taattaatat tttattttag 900 ttcttatagc tcaatttttt aataacactt ttttggtcct tgttctctaa aattatttaa 960 cgtaattaca tttagatctt ttaaaagtgt tacaaaactg caacggatag tgtaaattaa 1020 aaccgctaaa aagagatttg ggtaaatttt aatggtcata aataaataca acaacaacgc 1080 cttatcccac taggtgagtc ggttacatgg atcaacttcc gcctaatcgt cataaataaa 1140 taaagaaaag aaaacaagac tttgttgtgc catcaaccat ggccaaggca gaatcacgcg 1200 tgacaaactt tcataaacga attgagaacc ttattggcat tgggtcagag ccatcggcga 1260 ctgtgcttgt tttgttgacg gatttacatc atcaattcca gttttgtaac aattttgaag 1320 aatatggatg tttgcattag gcaactttag acatccaata ccaaaaggtt taacaattgt 1380 aactatatgg accaaaactc ctattttaaa caataggtat caaaagctaa ttaaactttt 1440 attaaaatag caagcctagg aagcatacgt gtgtatatac taaaattgct gcatgagtga 1500 actacacaaa ttcaatataa ttgttcacca tacttcagac aaaataatgt tggcccagtc 1560 ccatttaatg taagactcaa aatctatgcc agttgacccg aaccacacac aaacaatggt 1620 agaaatttgg agtacgtata ttggccacag tagcctactc aactgaaaag caaccaacct 1680 atgaactttg ctagctgttt ccaagccata gcaattaaac ttttcccctt ttacttttat 1740 tttcttgaaa tttttatgta ccaaggtggg ttggaaattt caaattggta aatcaggtta 1800 attcctttct attagctagg tgcactcaat cttccatgtt tatatacact ataacatttt 1860 atacagcatc accttctgta tgtctattta ttaatacaga aaagcataca catactatgt 1920 attgattgcc acagcctggt acaatcagcc acaactgttt ctcaaagtaa cttgtatatt 1980 gatattcatt tatacctaag ggtatgtctc tatcctgctc ccacaaatgt gggggaactg 2040 tgttgtagat ttcactttca agcgattgat attatatgtt caaggtaact cccctaatta 2100 atatattgaa tgagacaatt tgtgtaatgc taaataaggt ttctatatcc taaacttctt 2160 tagagtaaac atatgctaag aaaatggggt gtaccttcaa ttagtaccaa atttgcacac 2220 aagttgctta agcttaattt tgaaaaaaaa taaaagggtt tggagagaga ggatatttgg 2280 atgtattaga catgtgctca aggttttttc agaaaatttc aggctatatt acaagaagta 2340 gtgaattaat atcatgagta aatgcaccct gcaaaacatc aactaaccac acactcttcc 2400 cacccgctaa tatttacttc tcacttctgt gaactgtttt atattccatg ctttctccct 2460 tccccaaacc acacttcaaa atcctctggt aacccttatt 2500 <210> 11 <211> 2829 <212> DNA <213> Sorghum bicolor <400> 11 tctcctccga ctctggcctt tgtgtgcgtc tggactctgg agtgaatgct aatgaggttt 60 agtagacgag gtgctgtgca gatggaatgg ctactcacgc tgacattttt caggtgagag 120 atcagagttg gcaagggacg aaggaatgga ggttgggatg ggatgggatg ggatgggatg 180 ggatgggggt tgagagggac tgacagcacg ccaagctagc tgaattaagc aaagttaaat 240 aagcttatgt tggattagta gttggcatgt tgggcaggaa agcaatccag gttaaaaatt 300 gcttgagggt tgcaacctgc aaggctttgc ttcagagtgg ctcaaatttg ggtaggtttt 360 gatggcactc ttttgatttt gacaccaaat ctttctcgcg acattaacta tataatttcc 420 tattttaacc actttttaga aatgaaaatt attagcacaa taaagaagca tttgtaatgc 480 gatatttttt tttctgaatt ctcaagtttt ttttagatga agatccttca aagttgaaat 540 ttgttagtac caaataagcc cataaaattg gaatgagctt aacgtgaata ttccccggac 600 aacctagtca tgacacttgt tttggcagag tgaggaccca tggtgtttgg cattgcagca 660 cagtaccaat ctgccactgc ctgatgagtg atgatacaag agcatcatcg aaaagcaaca 720 gtagatgaca ccaggttttg gaagggcagc cagcagcttt ctgaccacca ccagacgtat 780 atgaaacaaa agcaatcagg agagcatatc aggtttggtg agaaaagttg tgttaacaaa 840 tgaagactgg aattccttga ctgatcgcaa gatgctacca gcagcaaaca cttagaatgt 900 aagctacagg cgtgtcagga gctaaagttt tgcagatagg aggtggctct cagcttctct 960 ctccgttgcc ttcaagtgaa gcctcggcaa aaccgggcat gaaacacgca tcgcccgccc 1020 caattccttg tgcagtccaa aaggaccaga accatttcac agtttcacag ttgcattgac 1080 aggaagtaat tcgaagacac gacataccga cgccagtaac aatataccat aaaaagcatg 1140 ggttgatggg ttggtcatcc aaacagaaga ttagctgata aacagacaaa gggatgtgaa 1200 aacgaacctt ttatggtagc aggtagcgat ggcaatgctc tatcttcttt tgcagatttt 1260 ctcatgaata tcactgctaa agccagtttt catcaactcc ataaatcgaa agcgaagggg 1320 cataacatca gttcctccat actttctacc cagtttcagt atttttgtgc ggtcgtagct 1380 aaccgcaggg agcattcttt tggcacattc aactattgga attcgatgac catgccaaac 1440 atcttaaaac cttccatcaa ccgaaagtac aagtacaaca tacaacggat atagatggaa 1500 actttcgttc ataaagtttg gtatcggcaa tgtatttact accacttaac agatgcatct 1560 ctggcatccc tgcagtaaag aagcagtaat aggaacagaa gaaaggtttt acaaaatctc 1620 acgggtatca tgatcatcag ttcattgcca ggtgtgacca tagataaacc acagcaagat 1680 gtaaccagga tcatgcagtc ccgcaccagt aggctaagtg ctcgtccttg cgagatgtgc 1740 taaagagccc accacatttc aggcagatga agctgctccc atcagcaaac gcatccagga 1800 tgatctgctt ccttccactc tcagcaagaa tgcaggcctc atcagaattc agcatatcga 1860 cagggtctga cccaggtcct tgtgaagggt tagtatttgt tgactgagcc tctgctatgg 1920 cacattgggc aagcaaagag gcaagctcat caacactagg agtggcctgc aatctctgcg 1980 catagagttc acgcgcacgg ttcattgtct gcatgagcgc ttgttcacca ccttgagatc 2040 ttatggccag gagaacctgc atagtttcat aacatgagaa tcaatataac agatacagat 2100 gaatgggcat gaaatgagac aaatgagtct caacgaaaca agataaagtt caaaaggtta 2160 ataattacca caaggctcat cattgggatc tatacgaaca aaattgaaac agccttccag 2220 aaggaatcaa aatagaaatt atgcatttca atagaccaga atgcttgtca cattaccaat 2280 gtttgattca ttaccagaac aaatcctaca gtgttattat gagggacaca tcaatcataa 2340 gatcccacag gaaacagcta aggcataatc ttatattgcg taaatgttgg tctaaaaaaa 2400 tcagttacac taacataggc acatagctat ggtgcaggca ggaagcacta atgacagagt 2460 gtggtgttgc agttgagaag taattagcct actactccaa tccacagaga agacgtgcat 2520 ttcattcgat ccatgtgata catcaggtaa tatgctatac atgtctaatc caaacaagat 2580 acggaaattc caatttaacc atcgcacact aatacatata gagaaagatt tgtttaggcc 2640 tatattgccc tgtactcctg tgtaaattta gtacagtata ctcaaataat tttatgaggg 2700 taccaaacac taattgtggg ctgtggcttt cttcttcaac ttttcccaaa ccaaccttct 2760 acttttaccc ttactcacca atataagggc tcgacggtca tttttaacag gtcaaatcca 2820 aaatgattg 2829 <210> 12 <211> 2567 <212> DNA <213> Oryza sativa <400> 12 aggtagcata agaccatcat ctttttccct ttctaatata ttatactacc tctatatttt 60 aatgtatgac gccgttgact tttcgtccac tatttgacca ttcgtcttat tcaaaaaatt 120 tacttaatta tcatttattt tattgtgact tgattcacca tcaaatgttc tttaagcatg 180 acataaatat tttcatattt tcacaaaaaa tttgaataaa acaaatagtc aaacattagt 240 aaaaaaaatc aatggcgtcc tatattaaaa tacggaggga gtagtttttc taaagtatcc 300 tgtgaatcac acgcgcatat gtagcttgca tagttacaca aaatatgcct cctttaaatg 360 ccggagctac caaaatgggc tcataatagc ctgaaattaa gtagtccctt caccgacaaa 420 acactagtta ctactgtatc atcaacaacg acctgaattt acagtagggg agaaacagct 480 tgaatataag ctgaaataag ttgaacccat accatcatcg ctagtgaaga gtttgattgc 540 agataattag ttatgcttag tataaaatgc aaaaatccat ctgatctata acaataggta 600 aataaatcac atctctaatt tatttaccca tgctggttct ttcagaaata atatttaaat 660 actttgaaag ttgcgttgaa gacagtcggc ccggatatta aggttcaact ttatttcttt 720 tttcatcaat tagtttatgc aattctgccc aatgatattg ttactagtaa attttacaga 780 cagtagccaa cactgtggat ttatttattc cataacccat gagagagatg tgataatatg 840 cacacactac taattcagta cagtgattta atatcactta tatctcatca tatcttggat 900 gtcatgtaga ataccaagtc aatgcaaaat ctattttctt tttttttcac cattaaatct 960 ttcaccaaat cataaaaaat cttatgtggc ggctagctca tttaatccta tgtaaaacat 1020 cttaggtcga gtattaggac tgtgccaaca gaaatactat gtgctagccc agctttcagg 1080 ggcccaacaa tcaccaaagt aactctgccc ttcggaaaat taaaggcagc cactcagaca 1140 cacagtgata tccagcaaat gcacgacgga tcacatggag tttcagcccc agggccaatc 1200 acatcttgcg caagtacagc cgttgctggt atcttcaacc attcctctat tatctcctgg 1260 cctggtcctg ctcagctgca actgtaacgg ttagttcagt cccatacttc ttccgtttca 1320 caatgcaagt cattctacca tttttcacat ttatattgat attaatgaat ctagatatat 1380 atatttgtct agattcatta acatcaatat aaatgtagga aatgttagaa tgacttatat 1440 tgtgaaatgg agggagtatg tacgtatggt ttcagagagt tcgactgttc cagaagttaa 1500 gggcaataga cagttggatg caggctacta gtcattacct ggtaattttc tgcctttttt 1560 tgtggtcatt gtaagtaatt cttgacagga gatcacacat gcatagctcc atttcatctg 1620 caatgtcaga cgctgtcaag attaaggagt aggagttttg tttataggag ggacgaaaat 1680 gtgagtgatg gatagcatga tgaaatcgca ggtgcgatat tgatgaatga ctgcagcagc 1740 attacactaa ttgctatata tattttttat ttaagagcag caaggtctgt ttgtaactaa 1800 aacagaaatg ttttcaatta aaaaaaacta acagcacaag gtctacagca taccaagtaa 1860 actaacgcta gctagaagca gccagataga tcagaaaaac tcgaaaactt tgcagcacaa 1920 ttctgtaatg taatgtgctg ataatgtata gcatgaaaga ctgcggcaca aatcagttcc 1980 aagaaactta caagaattaa caagcggaat tagaccaaag catcgctttt tacacaaaaa 2040 caattgtaaa atcaaatttg gtgcttccac ataacaactg ggccagggaa tcttaccata 2100 ctgtgcttcc attaaattga gaaattcaat tttgccacca cagcatgaaa tccagctcga 2160 ggcatggtta agtgtactga gtaggtggca agcaccgatc cccattaaat cagcagactt 2220 tcggggagga aatccattct gcatggaaag aagactgcca atatccatgg aagctaaaag 2280 tgccatggcc tcgctctaac ctacaaattt ggacacagat ttgagttaaa gaactcacaa 2340 tggaagcttc atcatgtgaa ttgaaagcga gcagatcacc gtcatgctga actagctgga 2400 cgaatcaaac cattaaatga tagacaacta ctaacagtgt caatggaact gtagagcatc 2460 tttcgcatgt atatataggc gcacaaaggc aattcagttc atcagctcaa gcacctcaac 2520 cagttcagtc atccattctg tcttctctgc agatactgag agccaag 2567 <210> 13 <211> 2500 <212> DNA <213> Oryza sativa <400> 13 aggtagcata agaccatcat ctttttccct ttctaatata ttatactacc tctatatttt 60 aatgtatgac gccgttgact tttcgtccac tatttgacca ttcgtcttat tcaaaaaatt 120 tacttaatta tcatttattt tattgtgact tgattcacca tcaaatgttc tttaagcatg 180 acataaatat tttcatattt tcacaaaaaa tttgaataaa acaaatagtc aaacattagt 240 aaaaaaaatc aatggcgtcc tatattaaaa tacggaggga gtagtttttc taaagtatcc 300 tgtgaatcac acgcgcatat gtagcttgca tagttacaca aaatatgcct cctttaaatg 360 ccggagctac caaaatgggc tcataatagc ctgaaattaa gtagtccctt caccgacaaa 420 acactagtta ctactgtatc atcaacaacg acctgaattt acagtagggg agaaacagct 480 tgaatataag ctgaaataag ttgaacccat accatcatcg ctagtgaaga gtttgattgc 540 agataattag ttatgcttag tataaaatgc aaaaatccat ctgatctata acaataggta 600 aataaatcac atctctaatt tatttaccca tgctggttct ttcagaaata atatttaaat 660 actttgaaag ttgcgttgaa gacagtcggc ccggatatta aggttcaact ttatttcttt 720 tttcatcaat tagtttatgc aattctgccc aatgatattg ttactagtaa attttacaga 780 cagtagccaa cactgtggat ttatttattc cataacccat gagagagatg tgataatatg 840 cacacactac taattcagta cagtgattta atatcactta tatctcatca tatcttggat 900 gtcatgtaga ataccaagtc aatgcaaaat ctattttctt tttttttcac cattaaatct 960 ttcaccaaat cataaaaaat cttatgtggc ggctagctca tttaatccta tgtaaaacat 1020 cttaggtcga gtattaggac tgtgccaaca gaaatactat gtgctagccc agctttcagg 1080 ggcccaacaa tcaccaaagt aactctgccc ttcggaaaat taaaggcagc cactcagaca 1140 cacagtgata tccagcaaat gcacgacgga tcacatggag tttcagcccc agggccaatc 1200 acatcttgcg caagtacagc cgttgctggt atcttcaacc attcctctat tatctcctgg 1260 cctggtcctg ctcagctgca actgtaacgg ttagttcagt cccatacttc ttccgtttca 1320 caatgcaagt cattctacca tttttcacat ttatattgat attaatgaat ctagatatat 1380 atatttgtct agattcatta acatcaatat aaatgtagga aatgttagaa tgacttatat 1440 tgtgaaatgg agggagtatg tacgtatggt ttcagagagt tcgactgttc cagaagttaa 1500 gggcaataga cagttggatg caggctacta gtcattacct ggtaattttc tgcctttttt 1560 tgtggtcatt gtaagtaatt cttgacagga gatcacacat gcatagctcc atttcatctg 1620 caatgtcaga cgctgtcaag attaaggagt aggagttttg tttataggag ggacgaaaat 1680 gtgagtgatg gatagcatga tgaaatcgca ggtgcgatat tgatgaatga ctgcagcagc 1740 attacactaa ttgctatata tattttttat ttaagagcag caaggtctgt ttgtaactaa 1800 aacagaaatg ttttcaatta aaaaaaacta acagcacaag gtctacagca taccaagtaa 1860 actaacgcta gctagaagca gccagataga tcagaaaaac tcgaaaactt tgcagcacaa 1920 ttctgtaatg taatgtgctg ataatgtata gcatgaaaga ctgcggcaca aatcagttcc 1980 aagaaactta caagaattaa caagcggaat tagaccaaag catcgctttt tacacaaaaa 2040 caattgtaaa atcaaatttg gtgcttccac ataacaactg ggccagggaa tcttaccata 2100 ctgtgcttcc attaaattga gaaattcaat tttgccacca cagcatgaaa tccagctcga 2160 ggcatggtta agtgtactga gtaggtggca agcaccgatc cccattaaat cagcagactt 2220 tcggggagga aatccattct gcatggaaag aagactgcca atatccatgg aagctaaaag 2280 tgccatggcc tcgctctaac ctacaaattt ggacacagat ttgagttaaa gaactcacaa 2340 tggaagcttc atcatgtgaa ttgaaagcga gcagatcacc gtcatgctga actagctgga 2400 cgaatcaaac cattaaatga tagacaacta ctaacagtgt caatggaact gtagagcatc 2460 tttcgcatgt atatataggc gcacaaaggc aattcagttc 2500 <210> 14 <211> 2602 <212> DNA <213> Populus trichocarpa <400> 14 aacagcctgt gctgtcaaag agaaaccatt gtgtattttc ttttcattac aaaatctacc 60 atcttactca ctagtacaag atctaaatac tccaacaaga gcttgatctt gttttcaatg 120 gtctggtgta agacaccatt aatttatttg aaatggggat atgagatata atcaaatagt 180 ggaggttgtg ggctagcagg gctcaaatct ttatggtgta ttggaccaaa caaaattggg 240 gcaggatatc attgttcttg ggctcaatat ttgttagctt gtccgatgag agaatgaata 300 ggaaggcacc ccccccctgt gtctcttctc ttccagattt aataaattag aggaaaaaaa 360 cttaatgtgc cgctcgggaa atgaatcatt tctagtggtt taagtcggga ccagctgaac 420 ggagtctctg aaaaaaaaaa aacgtagtcc tgaattcaaa cagcttaaag tctaagagat 480 aatgaaacac ggtgagggtt caatcccatg gcatggagca agttgagaca tgggttttga 540 tcagttttat tgaggttgga attgaatgtc acattcatca ctcggggatg ttgcagcacc 600 agatggcctt acaggaaaat catgtctatg tgcttttgag cctacttcta tagtttaatc 660 tcagcttgtc aaatgaagtc aacttgatcc tacagtatca ctgtatatga acttgttcta 720 taatcttgtc atattggtgg tgaatttttt ttctaatgaa ttttgtgaag accatgttat 780 aaaaagactc ctaacaatgc ctagatcacc acaataaaat ggcatggtga agaaaaaaat 840 aaaattatct ttaatatggc gagaaacata cttaaaacca agaaaatgcc aaatgaattt 900 tgggttgaag ttataaatta tgttatttac ttgttaaata ggttttctat gaaaagctta 960 aatgacatga ctctattata aaaagcatgc atggaatgga agaaagtcaa gtgttactca 1020 tttgaaagta attagaagca ttgactatat gcatgcagat gatcaagtaa ggaccaaatt 1080 agatgacaaa agcaaaaaat aatctttgtg agctataatc aaaagtcaaa ataatacaag 1140 tttcacaacc ctaataaata aaaaatgatg attaatagaa atgtcgaatt tgatgaagaa 1200 agagcatgaa attagaaggt agacgatgtt gagaagtatg aatttctacc agtttttgat 1260 gaagagatat aaagatcatc aataacttgt tacacctcca caatcatcaa taaactcaac 1320 ttcaccttta tcctcttctt cttttagtag aagttccagt agtggcagct cacttagtta 1380 atatagaaag atgaagagtc ttgatgactt gtataagata attaatccaa ttgatgatga 1440 tctatcacta tattgtcatc ttgctacatg taaatcttta gtgtttgtaa aagaaataaa 1500 tgatgaaaaa tagagaattg ctataaataa agagattgca tcaactgaga agaatgacac 1560 atgaaaattt gttcctagac caaaaagaaa gaagacaata ggctaagaag aagcaaccta 1620 tagtcacatt attaacttga gaagatgaat atgtatctac tacaacaagt gtttgtcatt 1680 ccatatggct aagaaaatta ttgaaagagt tatgaatgcc acatgagaag cctacaaaaa 1740 tttatataaa caactcatta gtcattgctt taactaagaa tccaatattt cataactgaa 1800 gtaagcacat acacacaaga tttaatttcc aataagattg tattacaaac aagaaagttg 1860 aagtcaaata tataaaaaca caagatcaag ttatagatat ttttataaag ccactcaaat 1920 atgatgtttt tgtcaagatg agaaatatgc tatgagttat aaagaaatca attttaagag 1980 gggatgttga aagtaaacta gattttagtt tttttgcaaa atagaagaat cggtcgatcg 2040 gttcagaaac aattaaccaa taaagacgac ataatacatt ttcttatttg cctagaacct 2100 gaggtttata aataaaattt taatctaagg aataattaat atatgtaaag agaaaatcta 2160 aggaataata aaattgtttt tttattgaat cctcttcttg ttttttagtt ttacttatta 2220 ttttgctgtt aataaactcc tgccacactt attattattt tttttttcca atataataat 2280 tcatttcgta gtaaccttgg caaaacctcc atggcaatgg tggcaattca cacatcagag 2340 ttcttaaaac gagagaacaa tccattagca agagtgagct tgggcatgtg gcgggggttg 2400 gattaattgc attatcgcat cagtgcaagt cattaatagc acaaattaat gctccggcaa 2460 caaatcaact aaccacatac tccttcacct gctaatattt acttcactct gtcaactcta 2520 tataatcccc gctttcttca ctgaagaatg cacctttcag ttatatccca acttacttct 2580 agtgttctcc aagaatattg ag 2602 <210> 15 <211> 2500 <212> DNA <213> Populus trichocarpa <400> 15 aacagcctgt gctgtcaaag agaaaccatt gtgtattttc ttttcattac aaaatctacc 60 atcttactca ctagtacaag atctaaatac tccaacaaga gcttgatctt gttttcaatg 120 gtctggtgta agacaccatt aatttatttg aaatggggat atgagatata atcaaatagt 180 ggaggttgtg ggctagcagg gctcaaatct ttatggtgta ttggaccaaa caaaattggg 240 gcaggatatc attgttcttg ggctcaatat ttgttagctt gtccgatgag agaatgaata 300 ggaaggcacc ccccccctgt gtctcttctc ttccagattt aataaattag aggaaaaaaa 360 cttaatgtgc cgctcgggaa atgaatcatt tctagtggtt taagtcggga ccagctgaac 420 ggagtctctg aaaaaaaaaa aacgtagtcc tgaattcaaa cagcttaaag tctaagagat 480 aatgaaacac ggtgagggtt caatcccatg gcatggagca agttgagaca tgggttttga 540 tcagttttat tgaggttgga attgaatgtc acattcatca ctcggggatg ttgcagcacc 600 agatggcctt acaggaaaat catgtctatg tgcttttgag cctacttcta tagtttaatc 660 tcagcttgtc aaatgaagtc aacttgatcc tacagtatca ctgtatatga acttgttcta 720 taatcttgtc atattggtgg tgaatttttt ttctaatgaa ttttgtgaag accatgttat 780 aaaaagactc ctaacaatgc ctagatcacc acaataaaat ggcatggtga agaaaaaaat 840 aaaattatct ttaatatggc gagaaacata cttaaaacca agaaaatgcc aaatgaattt 900 tgggttgaag ttataaatta tgttatttac ttgttaaata ggttttctat gaaaagctta 960 aatgacatga ctctattata aaaagcatgc atggaatgga agaaagtcaa gtgttactca 1020 tttgaaagta attagaagca ttgactatat gcatgcagat gatcaagtaa ggaccaaatt 1080 agatgacaaa agcaaaaaat aatctttgtg agctataatc aaaagtcaaa ataatacaag 1140 tttcacaacc ctaataaata aaaaatgatg attaatagaa atgtcgaatt tgatgaagaa 1200 agagcatgaa attagaaggt agacgatgtt gagaagtatg aatttctacc agtttttgat 1260 gaagagatat aaagatcatc aataacttgt tacacctcca caatcatcaa taaactcaac 1320 ttcaccttta tcctcttctt cttttagtag aagttccagt agtggcagct cacttagtta 1380 atatagaaag atgaagagtc ttgatgactt gtataagata attaatccaa ttgatgatga 1440 tctatcacta tattgtcatc ttgctacatg taaatcttta gtgtttgtaa aagaaataaa 1500 tgatgaaaaa tagagaattg ctataaataa agagattgca tcaactgaga agaatgacac 1560 atgaaaattt gttcctagac caaaaagaaa gaagacaata ggctaagaag aagcaaccta 1620 tagtcacatt attaacttga gaagatgaat atgtatctac tacaacaagt gtttgtcatt 1680 ccatatggct aagaaaatta ttgaaagagt tatgaatgcc acatgagaag cctacaaaaa 1740 tttatataaa caactcatta gtcattgctt taactaagaa tccaatattt cataactgaa 1800 gtaagcacat acacacaaga tttaatttcc aataagattg tattacaaac aagaaagttg 1860 aagtcaaata tataaaaaca caagatcaag ttatagatat ttttataaag ccactcaaat 1920 atgatgtttt tgtcaagatg agaaatatgc tatgagttat aaagaaatca attttaagag 1980 gggatgttga aagtaaacta gattttagtt tttttgcaaa atagaagaat cggtcgatcg 2040 gttcagaaac aattaaccaa taaagacgac ataatacatt ttcttatttg cctagaacct 2100 gaggtttata aataaaattt taatctaagg aataattaat atatgtaaag agaaaatcta 2160 aggaataata aaattgtttt tttattgaat cctcttcttg ttttttagtt ttacttatta 2220 ttttgctgtt aataaactcc tgccacactt attattattt tttttttcca atataataat 2280 tcatttcgta gtaaccttgg caaaacctcc atggcaatgg tggcaattca cacatcagag 2340 ttcttaaaac gagagaacaa tccattagca agagtgagct tgggcatgtg gcgggggttg 2400 gattaattgc attatcgcat cagtgcaagt cattaatagc acaaattaat gctccggcaa 2460 caaatcaact aaccacatac tccttcacct gctaatattt 2500 <210> 16 <211> 2134 <212> DNA <213> Setaria italica <400> 16 tttggaccat gtttagttcc tccaaaactc ccaactttag cactatgcaa aaagaagatt 60 ctccatcaca tcaaacttgc ggtacatgca tggagtacta aatgtagacg aaatcaaaaa 120 ctaattgcac agttttgttg tactttgcga gacgaatctt ttgagcctaa ttagtcaata 180 tttggacaat aatttacaaa tacaaacgaa acgctagtgt gctacagtgc tacaacagta 240 attttgcacc tccaaacttt ggccaaccaa acaagatcct atatatcgga gttcgttcta 300 cgacgacttg cactacatgc cccgtttaaa tgctcagcta gtcagctacc aaaatgggct 360 cataaagtca taatagcagc ggactccttc caccgacaaa ctcccaccct cctctagtca 420 cccgcagcat gccaacatga atttcagctg aaacagagct gcaccaccac ccaagcacgc 480 aaacaaattt gagcaagtaa aattaattgc accgcaagaa cagtaaatgc aagtactgtt 540 agtatattcg gccaatgaga aatgaacgca ggagagatgt ttttcgtaca tgtgctttct 600 tgaaggaccc ctcgcatcac cactcatcaa tggaactgcg tgttcaaaaa tggcggccac 660 agagcagcag ccgctagctt ccgtctgtcc gggcccccga actctctgta aagctccggc 720 agatccacaa gagatcacat ggcagcccga tctccccggg gccaatctcc tcttgagcaa 780 gaccagctgc tggcctctcg cgtccttgtg cttcctcctt gattggaagg cagcctggtg 840 ttaaaggtga gagcacagta aatttggttt ggtactcctt ccgttccaaa ttgtaggtca 900 ttttaatttt ttagtttata aatattatta tgcacctaga cacatgtttc tgtagttgtc 960 ttcctgtaag gaaaaggacc ttgcaaattg aacccatgta ctgatggtca tttctgaact 1020 tcgcacatga ggtcagtacg gtcagtccat gtcgtagtaa ttccgaagga aactgcacat 1080 gaatgactca tgtatgatgt atattagtaa aatgctgcca tgatgtgaac cagcaatacg 1140 atagcatgtg cgacgatgtt gtacaaaaca gaggaggccc ttgtaaaaga cagacggcat 1200 tacattcagg ggctaaaaac atggtaccat acaggtgaga tatgatggat gaatgcgggc 1260 agcagcccac tgcgactaca gtatacgcac taaaacctcg gcagatgcaa tcagaataat 1320 acacagaaac gcagaggact atcagactat gcaccagtat tctgacagag tataagcaca 1380 cttttgaata tgcagcagta aaagatgcag tgaaatcagt tttaaagaag ttaccagatt 1440 ttccagtaaa gatatgagac ttgtgatgaa atctggccaa atcctgtgcc tttcagttgg 1500 atcacaaaat accttcagcc gctccatagt tattcagaac aaactcgagt tagatccctc 1560 gaccctctgt ttcgctggac cagccaaacc catgtttcca ttaataagtt cgatagattc 1620 ggtatttcaa ttcagggatc agatcgccga atccagctcg aggtatagtt aggtacactg 1680 agtaggtggg aggcaccgat tccgattaaa tctgcagtct gggaatagaa aagatcctca 1740 ttttctgtca aaagatgcct gcccaaaatc cacgaagctc taagctatca cagttcacag 1800 ccttagtgcc cttttccttt ttttttgcac aatatttgta gaaaagaatt tgggctaaaa 1860 tactcagtac cttcattatg ctagttgaga gcaagcagat cactgccgtg acaagctgga 1920 caaaccaaga tcattaaatg ttggccaact actaacagtg tgatgaacat gtacacatct 1980 ttgcacgcaa tagcttcaat ggaactgtgg agcactgctc gaatgtatat atatgtgcct 2040 gaagggaact cagttcatca actcaagcat ctctaggttc tcaaccagtt cttaggccac 2100 atattcagtc ttgtctgtag acgttgagag caag 2134 <210> 17 <211> 2056 <212> DNA <213> Setaria italica <400> 17 tttggaccat gtttagttcc tccaaaactc ccaactttag cactatgcaa aaagaagatt 60 ctccatcaca tcaaacttgc ggtacatgca tggagtacta aatgtagacg aaatcaaaaa 120 ctaattgcac agttttgttg tactttgcga gacgaatctt ttgagcctaa ttagtcaata 180 tttggacaat aatttacaaa tacaaacgaa acgctagtgt gctacagtgc tacaacagta 240 attttgcacc tccaaacttt ggccaaccaa acaagatcct atatatcgga gttcgttcta 300 cgacgacttg cactacatgc cccgtttaaa tgctcagcta gtcagctacc aaaatgggct 360 cataaagtca taatagcagc ggactccttc caccgacaaa ctcccaccct cctctagtca 420 cccgcagcat gccaacatga atttcagctg aaacagagct gcaccaccac ccaagcacgc 480 aaacaaattt gagcaagtaa aattaattgc accgcaagaa cagtaaatgc aagtactgtt 540 agtatattcg gccaatgaga aatgaacgca ggagagatgt ttttcgtaca tgtgctttct 600 tgaaggaccc ctcgcatcac cactcatcaa tggaactgcg tgttcaaaaa tggcggccac 660 agagcagcag ccgctagctt ccgtctgtcc gggcccccga actctctgta aagctccggc 720 agatccacaa gagatcacat ggcagcccga tctccccggg gccaatctcc tcttgagcaa 780 gaccagctgc tggcctctcg cgtccttgtg cttcctcctt gattggaagg cagcctggtg 840 ttaaaggtga gagcacagta aatttggttt ggtactcctt ccgttccaaa ttgtaggtca 900 ttttaatttt ttagtttata aatattatta tgcacctaga cacatgtttc tgtagttgtc 960 ttcctgtaag gaaaaggacc ttgcaaattg aacccatgta ctgatggtca tttctgaact 1020 tcgcacatga ggtcagtacg gtcagtccat gtcgtagtaa ttccgaagga aactgcacat 1080 gaatgactca tgtatgatgt atattagtaa aatgctgcca tgatgtgaac cagcaatacg 1140 atagcatgtg cgacgatgtt gtacaaaaca gaggaggccc ttgtaaaaga cagacggcat 1200 tacattcagg ggctaaaaac atggtaccat acaggtgaga tatgatggat gaatgcgggc 1260 agcagcccac tgcgactaca gtatacgcac taaaacctcg gcagatgcaa tcagaataat 1320 acacagaaac gcagaggact atcagactat gcaccagtat tctgacagag tataagcaca 1380 cttttgaata tgcagcagta aaagatgcag tgaaatcagt tttaaagaag ttaccagatt 1440 ttccagtaaa gatatgagac ttgtgatgaa atctggccaa atcctgtgcc tttcagttgg 1500 atcacaaaat accttcagcc gctccatagt tattcagaac aaactcgagt tagatccctc 1560 gaccctctgt ttcgctggac cagccaaacc catgtttcca ttaataagtt cgatagattc 1620 ggtatttcaa ttcagggatc agatcgccga atccagctcg aggtatagtt aggtacactg 1680 agtaggtggg aggcaccgat tccgattaaa tctgcagtct gggaatagaa aagatcctca 1740 ttttctgtca aaagatgcct gcccaaaatc cacgaagctc taagctatca cagttcacag 1800 ccttagtgcc cttttccttt ttttttgcac aatatttgta gaaaagaatt tgggctaaaa 1860 tactcagtac cttcattatg ctagttgaga gcaagcagat cactgccgtg acaagctgga 1920 caaaccaaga tcattaaatg ttggccaact actaacagtg tgatgaacat gtacacatct 1980 ttgcacgcaa tagcttcaat ggaactgtgg agcactgctc gaatgtatat atatgtgcct 2040 gaagggaact cagttc 2056 <210> 18 <211> 3000 <212> DNA <213> Arabidopsis thaliana <400> 18 ttctgtaata aatacgtatt agaaaatgat gaagatatta gatattctct cgaaatcaag 60 tcgagggcca ttaaaattga tgattattag aaaaaaggtt ataaatactt agttggaaga 120 cctagctagc taataactaa gaaaattaat agaagaaact ctctcttatt gatatgtcgt 180 cgtccatgct tctatattgg atatagttct cgcatatgag taaatctact atcattaata 240 caaaaacaat aatatcaaac gcttaattag catttgaaca ataaaaatca tttatgtatt 300 catgcataat ttagacacac acacacacgc acacgcacga atatatacat gttgtagtac 360 actagcactt aggtctaaat tttgagaact acgcaatatg acaagtaact aaatctttgt 420 taacctaact aatgcgttgt tgttgacata gatgaggtac gaaatggttg taatatgaat 480 tcttatacac attagtatat gtggaagcca agtagccaac gaaacacttg catgtaaatt 540 tttttgatta ttctttacaa gcttaaacgt tttttattta acctatagag gtaaaagaaa 600 aacagtgatt ttggttttga cttatataaa ccgaaactct ttctatcact ctatagagat 660 aaatatacaa agaaatgagt aagtttctct cactatagca ttaatggagc tttagatcat 720 cataaatgca ttgcaccctc tctatgcgct catcaataac tccttttaaa tttcttattg 780 caactccacc tgttcttttc cttcctctct tgtcctcttc tccttcttct tcttcaacaa 840 caaacttcca ttgttcttaa tacctagcta gctcttcccc ggtttgagat tatcccaaaa 900 taacaccggt tctcgggtaa ccggtcggtt tttccggagc aaccaaccct tttacttctc 960 tttatcattg tctcagatct tctgttcttc aaaggcaaaa atgggcataa accaaagatc 1020 attgtgaatt agggtttcac tgtgtctcca aaagtaagat ctctattatc aaaaaagatc 1080 tttctctctc tacacatata tgtatgcata tgcatttgtg tgtatgtatc ttaggttatg 1140 tttatagatc ggatccatat tgcatgttgt tgagactctt agatcttcta tttttttgtt 1200 tatggtgttt tttggttctc aagatctcga tgcctatgat ttgttttttt ttctagatta 1260 ttttagttta atttaattta atgttttttg tgtctttttg cggagactta tgatctaaga 1320 taaataaagc gttaccaaaa actttcttgg agttagcagt ttcaaaaggg gtttgacgaa 1380 atctacttgg tggttgtgtc acaaaaaaaa gaccattgac ttattgtcaa cgctttaatt 1440 aaccctatat actatatact atttttaatc gcttttaatt aatcaaccct atatatcctg 1500 tattaaacta attaaactta gcctttttca ttagttttgt tactggaaac ggttcgtctt 1560 tagtgcacaa agaccaaggt caatttaatt ctttaataaa ttcaattgag agatcagaag 1620 cttgtgaatc tggttctctc tctctcttct cttactagct tttgagtcga caaaagagag 1680 agaaaagatc attaaactcg aatccaaaac ttcaaccaac aaaagggaaa aaaagaaaaa 1740 ataactacaa agagatctat ctttagggtt tcaaaacttt cttgagtttc agatcgaatt 1800 gagtaagtat ttatattatt taagtgttct tcttactgta tcatttctga ccatacacca 1860 aactagggtt tcttaaagat cataactctc tctctctcgc ttcttcctac actagatctg 1920 tccgatagat ctgatgcttt gagaagtctt gtgactttca agattgattt ttctttttgt 1980 ttgtttgttt atatgtacac atacttggag atagatatac tctcatatat gttactgtgc 2040 atatggatgg atccatctta agtgtgaata aaaactaccc aaatctcatt tttgtttgtt 2100 tcttatctca tttgttgttt ttgtaaggcc gtcattataa atctaaaaca atttatccgt 2160 tttcgtaaag aaaatcgctt tccaaaaaaa aaaactttga agtaaaaata tcattttggg 2220 tctatattca aatctctcct catatttcca tctcattatt tgtaaaatta atatattagg 2280 tcattatcat attgattata gagtttatat gttatgttat aaagtaaaag ttatatatgt 2340 aaatattacg tttgtaatat atatatttat acatatgtac gtatacataa tttgtatgta 2400 tatgtgcctc atttttaaca aatattatcg ttaatcttta gtttcattat aattttttag 2460 ggatgtatac attatataaa tattttatgt tatttatatc tgaaatttct atactataac 2520 atcttagata gttttcatac gtaattaaga gttttaatta atagttacat aattatgatt 2580 agatgaattt atctataaat tgtatgcaaa aatatataca tatccaaccg aaaaaatgga 2640 gagtaaacaa aaacctaaca attaatggtt tagtacttgt gaaaatgata aaattattaa 2700 tttaatatat agtttcataa cagctagaaa taactttccc ttaatataat tattttcttc 2760 catttccata atgattttct tcttcttcat tctgtataca tatatgagcg tttcatatat 2820 aatcttttac taattcgatt gtgattttga ttttgggcag tggaagatat acttggagaa 2880 gaaaatcaaa tcacgaaaat atattgatca gtgccttgaa ggaaaccaaa gttgacaact 2940 ccaaaaatcc atttcgttat taatatctaa ctgagtgatc atagtcaatc atccataaca 3000 <210> 19 <211> 6237 <212> DNA <213> Glycine max <400> 19 taatattatt atcagtggag tcacgtagga aaatgaaagt agaaaaagtg tttgcggaga 60 agaagaacat aatgttgtga aaaattagga ttaataattt ttttatttaa ttttttaata 120 actaatgatt ttgattaatt tataattaac ttaataactc aattaattat aagtatttat 180 taataatcta tcagaatgat caaaatcact aatttataag atttgaggat caaatgttat 240 aattaaaatt caggagacaa caatcataaa tttgagaaac tagatgacta aaaattacaa 300 ttaaaattta ggaattaaaa tcatggattt gaaaaattag gatgaccaaa attataattt 360 aaccttcttt tataagaaga aatttaaaag tctccttgca ttaaatattt ttttgaaaac 420 attattaatt atgaaccatt tttctgaaat taggatgacc aataggtaga atattaatat 480 ttttttcctt ttcatatgta gagaatttac agaaatctta ttgtgtacta tctttacatc 540 tataaacaat aaaaggtaga gcaataaaca tatttacaga ttaattaaac gacataaaga 600 caacaaatat actaaacaaa aagaataagg acatgtttca ttactattat aggtaatttt 660 gaaaataata tataaattta gaataaatat ataattatta atttaattaa ttattttttt 720 aatctttatg aattagttga ttgaatttta taaaaagatc aaaagggagc ggttaatttg 780 tagatgactt gttattcatg agaaactaaa gaaaaataag aattaatgat ataaatatta 840 atcattcaat tcatcttgta tttactaaat caatcaacaa attaatttaa atcagaaatc 900 tgatgttaat taaaatgaca ttattttcta aaatattgtt aaatcctttt actattaatg 960 aattatataa gaatcaaatt taaataaata taattattgt ttagtgacta attttatctt 1020 ataaaatgag tttaatattt atacactcat agtatacaag agttttacat tatcatccaa 1080 tcatgaattc cgtttgaatt actttaaaat aattatttga aaagtcaata aatttaatat 1140 atatgatgag tttaattaaa tgaatatata aaactttttg cataatatca atatataata 1200 attttctctt ataaaaatag tatgtaagat aattttctct cattatgatt atatactaat 1260 acatttgtta aaaaaattat aataacgata tactgtatca aaattaaact cttataaaat 1320 tgttacgaaa taattaaatg ttaaaataga gtctttctat tgtaaatata tttaaatcaa 1380 tatttaacac caataaacaa taaacatttt gtttataaat attttaatta tggctaatgt 1440 taaccctttt tattgtgatc cacaaaatta tattgtcatg atttttttac gctctcgtat 1500 tctttttttt ttttgactta attatttttt taacccctga atttaagtcg tgtatttttt 1560 ttagtcttta aaatttttta aaaaaattct ctgaattttg acaaatgatt tttttttgac 1620 ataataaatt gatattttat gtcaattttt agtactttgt gaaatttttt tagtatttcg 1680 taatagtttc aaaatgaaaa tttaaaataa actaatatta tgatttaatt tacagaatca 1740 atccattttt ttgtcaaact catttcaaca tcttgtaaaa taagtataat aattaatctt 1800 tactatcaaa actagtttta agcccggctt aactaattat tattttagtt tctaaatttt 1860 ggatttgtga tttttttagt ccttaaaatt ttaaatttat ttctttgttt ctgaattttg 1920 ataaattatt ctttttagtc agagccatgt aaattgatat tttgtgataa tttttaacta 1980 cttaaatttt taataaaaaa accatcacaa agtgttaaaa accaccatat aatacagtgt 2040 tagtttataa gtaagagatt aaaaaataaa tttgtcaaaa tttaaggatt aaaaaaatat 2100 ttttaaattt tagagattaa aaaaatacac tccaaattca agaactaaag caataattaa 2160 gttttaattt tttaaccaat aacctaataa cactcaccaa ttagcaagac cgtgaatact 2220 tgttaacttt ttaatttgaa aggcaagagt agaaggcagt gtaaggggct agggttgaga 2280 aatataagag tcacttcact ggacctagct cacaaaataa aagttaaagc atggaacgat 2340 atcgcgctct aactctcttc tattcatgta taaccattta attaaattta caccagtcat 2400 ccctccataa atgcttctac accctctgca ataacttcat gctgccaaac cctctcccac 2460 ttgttctact tattcatttt atctcttcct gcgtcgtttg gttctatctc tatttcgctt 2520 tggcccacac taaactcaca acttccccat caacaactcc ttcacttccc taattcccca 2580 cacacattct tgtttctctg atgacccaca acacatccgt ttcagtcaac aacataacta 2640 attaaggttc attccactct caaacgctta aggccccctc ttgcatctac gcattcttaa 2700 ttttagcttt gacttgcttt gcttagctta gttaatttcc gagtgagaga aaggggtaac 2760 ggctacgttc ccccgcattg aaattggtta cttgacaagc gttatagtaa tcatctgttt 2820 tgtcaaacct ttcacactga ctcaacactc tacccttatt ttgtcttttc tgatttccct 2880 agcattgcaa gttttgcgtg taagataaga gtagtaaggc caaggccagg gaaagtcagc 2940 agtgtcctct tgcaacctta acataagaat gaatctccag acaaacactt gaaagtgctc 3000 gaaatcgatc gatcagagac aaaacagcga cacgaggggc ctggatgctt ttattatcaa 3060 tttttgcttt gcgtggcggt tccataaatc ccaattgcaa gtcccatcaa actttagaga 3120 tggaaagcgg ttgattcatt atattgcagt cgtcttcgtg gttaatctat caggtgatca 3180 cttccttaat ttcttatttt ctctcataat taaacagagg tacaacaaat tttctttttt 3240 gagtaaaaca gcaagttgat taaatggaaa aactttaaca ttttattcat tctcgatggg 3300 gagaaaagtc attctggctt agaatttttt tacatatata cagttgctat tgttatcaca 3360 tatgttcatc ttaatgtttt attgccaaca aagtttggta ttttagcaat ctacaagtat 3420 ttctgaaaat gtttttttag gtgtgtcatg gctttggttg aagctcacct tatttaaatt 3480 tttttttttg aaaataatct acgtttcatc tcgactaaaa ataatcacac attaaaatat 3540 ataagtgaga gagacaattc ttattcctta agttaatttt taggattaag ttagacttct 3600 cacattctaa gatcttatta atgtgagaga caactctcat tctctcactt actttattgt 3660 aagaatttta ttctttcttt atttttatga taagaaagtg ttaattttta atgtttattt 3720 tagagagaaa aaaagtttct attgtgaggc ccaggagaaa aataaattcc ttacctcccc 3780 aaattggcat gaaatgggtg aggggaaaaa tgatctctta ctaattttta ttttagtttt 3840 cttaatatat atatctttaa aaatagttaa atataaaaca attttatttt taaagataag 3900 tatatttttc tattagaatg aaaaatataa ctttttataa ggtgtttggc tatttggtgt 3960 ttccataagc atcgagaata acttaaactc tttatccttt ttctaaatat gcaaattaaa 4020 tcaaactcaa ggatcatcct ataccttttt tagttagcat gttattggta taaaatatct 4080 acttgtttta ccaataatca attttcatcc acaaatctta aatctaagac tttatttatt 4140 taaaggaatt gagtcaaata taacttcgac caacatcttg gaaatattta tttatttatc 4200 taagtaatta caagaaaaaa attctctgcg tttttgttat ttccatttat caaaataacg 4260 catctttgct actctattaa tttttttttc tttttttctt tcttattttt tctgtatttt 4320 atttctaaat caaatagaac attaatatgt ttctatctaa tcttttgtta cccataataa 4380 tcataaactt aaaaaaattt ctgtgtaaac taaacttgta gttttaaaac aaaaaataaa 4440 ttccatttgt ctactcaatt aataaataat tctaaattat ttaatattct ctcagtgttt 4500 atattttaac atctttaaat atcaaacact ctattaacac tcttaattta tctctatcat 4560 tcttttagta tctcattgtc tatcatatcg atgtttctct ctcttttatt tccatctctc 4620 aagatatcaa ttaaatttgg gtgtttgaag actttttacc atattaattt tcctactttc 4680 tttttatttt ttcatcttgc tcaatcattc gacttaactg acttgactac tttcttttta 4740 tatggtgagt atttaatgtg ttttatattt taaaaacatt aaatatttta tatttatttt 4800 tcatttaata cttttttcgt atggtaataa tattcttttc atttaatact ttttttgtac 4860 ggtaacactt ttttcaattt caaccaatac accttcacgt aaaaatatta aaaaaacaat 4920 taatttgata aataatttag aaaaagatat tagttaaaaa atacatgtaa tacttttaat 4980 caataccgat tatattttgt ctttatacta attaatcaat accgattata tttcctcttt 5040 aagtctttca ctataaattg aattcgagta atgttgtatg agaaatgcaa ggaagacatt 5100 ttttaacaaa ctcttgatga tttccttaaa aaaaatactt cttttataat tgattgaaat 5160 ttataggaaa tcataatttt ttggaatgct aggaacattc tctttggcac actcctttaa 5220 agattctttg tatatacgat tgactaaagt ttattgaaca attttgatag gttttaattt 5280 ttatttaata taaatcagga gagagtatga aaaataaaat gtaataaaaa tgatgatttt 5340 cattaaattc taaggaaata ttaaaaaaaa aagcgttaaa gaaagtgatg ttaacattta 5400 tggtattcac taccaagcac attaattagt aatcttttta atttgagttg tgatagttgt 5460 tttatccttg taaaaaaaat tctccaataa aaagtattag tatttatttt ttatataaga 5520 ttcaatttat aattttttat tttgattatt tttaaattaa attgcctcta aataagttat 5580 tatattggaa aaattaattg agatgagaga atttattata tgtcagaaat acaaatgtat 5640 ttaatattta cttctacaaa aaataattta aatacttaat tgaatatgtt ttttaggtac 5700 atccttaatt gaatatgttt aaaaatttct aagaaatttg agggtaatag ttaataagat 5760 gcatagtttt ctttttatta ttactgatag ttattaagta aaaatatcta tatttttcat 5820 atttttagat gctaaaagta ttggattctt aatatttatt attttaatac aatttaaaaa 5880 tttatagaaa gttaatatta gaaaaatata attaatttaa aattaagaat agcattgctc 5940 atttactatt ttactaaaaa tataaaatat tatctgtcaa gaaaatatta aatattaact 6000 atttacctta ggtagttatg aacattcaat gtattaaact tctaacgtat attatattga 6060 ttattgaagt tgttcaaaaa atattcttaa gggcactcgc tatagggcta cacaaactag 6120 cctacttctt gctttcccac atttttgctt ccgtcgggcg agttaaatgt tttagaattc 6180 ctggtttggc agagactagc tagagaagaa aattgcggct tccgaattat ttaagga 6237 <210> 20 <211> 6192 <212> DNA <213> Glycine max <400> 20 taatattatt atcagtggag tcacgtagga aaatgaaagt agaaaaagtg tttgcggaga 60 agaagaacat aatgttgtga aaaattagga ttaataattt ttttatttaa ttttttaata 120 actaatgatt ttgattaatt tataattaac ttaataactc aattaattat aagtatttat 180 taataatcta tcagaatgat caaaatcact aatttataag atttgaggat caaatgttat 240 aattaaaatt caggagacaa caatcataaa tttgagaaac tagatgacta aaaattacaa 300 ttaaaattta ggaattaaaa tcatggattt gaaaaattag gatgaccaaa attataattt 360 aaccttcttt tataagaaga aatttaaaag tctccttgca ttaaatattt ttttgaaaac 420 attattaatt atgaaccatt tttctgaaat taggatgacc aataggtaga atattaatat 480 ttttttcctt ttcatatgta gagaatttac agaaatctta ttgtgtacta tctttacatc 540 tataaacaat aaaaggtaga gcaataaaca tatttacaga ttaattaaac gacataaaga 600 caacaaatat actaaacaaa aagaataagg acatgtttca ttactattat aggtaatttt 660 gaaaataata tataaattta gaataaatat ataattatta atttaattaa ttattttttt 720 aatctttatg aattagttga ttgaatttta taaaaagatc aaaagggagc ggttaatttg 780 tagatgactt gttattcatg agaaactaaa gaaaaataag aattaatgat ataaatatta 840 atcattcaat tcatcttgta tttactaaat caatcaacaa attaatttaa atcagaaatc 900 tgatgttaat taaaatgaca ttattttcta aaatattgtt aaatcctttt actattaatg 960 aattatataa gaatcaaatt taaataaata taattattgt ttagtgacta attttatctt 1020 ataaaatgag tttaatattt atacactcat agtatacaag agttttacat tatcatccaa 1080 tcatgaattc cgtttgaatt actttaaaat aattatttga aaagtcaata aatttaatat 1140 atatgatgag tttaattaaa tgaatatata aaactttttg cataatatca atatataata 1200 attttctctt ataaaaatag tatgtaagat aattttctct cattatgatt atatactaat 1260 acatttgtta aaaaaattat aataacgata tactgtatca aaattaaact cttataaaat 1320 tgttacgaaa taattaaatg ttaaaataga gtctttctat tgtaaatata tttaaatcaa 1380 tatttaacac caataaacaa taaacatttt gtttataaat attttaatta tggctaatgt 1440 taaccctttt tattgtgatc cacaaaatta tattgtcatg atttttttac gctctcgtat 1500 tctttttttt ttttgactta attatttttt taacccctga atttaagtcg tgtatttttt 1560 ttagtcttta aaatttttta aaaaaattct ctgaattttg acaaatgatt tttttttgac 1620 ataataaatt gatattttat gtcaattttt agtactttgt gaaatttttt tagtatttcg 1680 taatagtttc aaaatgaaaa tttaaaataa actaatatta tgatttaatt tacagaatca 1740 atccattttt ttgtcaaact catttcaaca tcttgtaaaa taagtataat aattaatctt 1800 tactatcaaa actagtttta agcccggctt aactaattat tattttagtt tctaaatttt 1860 ggatttgtga tttttttagt ccttaaaatt ttaaatttat ttctttgttt ctgaattttg 1920 ataaattatt ctttttagtc agagccatgt aaattgatat tttgtgataa tttttaacta 1980 cttaaatttt taataaaaaa accatcacaa agtgttaaaa accaccatat aatacagtgt 2040 tagtttataa gtaagagatt aaaaaataaa tttgtcaaaa tttaaggatt aaaaaaatat 2100 ttttaaattt tagagattaa aaaaatacac tccaaattca agaactaaag caataattaa 2160 gttttaattt tttaaccaat aacctaataa cactcaccaa ttagcaagac cgtgaatact 2220 tgttaacttt ttaatttgaa aggcaagagt agaaggcagt gtaaggggct agggttgaga 2280 aatataagag tcacttcact ggacctagct cacaaaataa aagttaaagc atggaacgat 2340 atcgcgctct aactctcttc tattcatgta taaccattta attaaattta caccagtcat 2400 ccctccataa atgcttctac accctctgca ataacttcat gctgccaaac cctctcccac 2460 ttgttctact tattcatttt atctcttcct gcgtcgtttg gttctatctc tatttcgctt 2520 tggcccacac taaactcaca acttccccat caacaactcc ttcacttccc taattcccca 2580 cacacattct tgtttctctg atgacccaca acacatccgt ttcagtcaac aacataacta 2640 attaaggttc attccactct caaacgctta aggccccctc ttgcatctac gcattcttaa 2700 ttttagcttt gacttgcttt gcttagctta gttaatttcc gagtgagaga aaggggtaac 2760 ggctacgttc ccccgcattg aaattggtta cttgacaagc gttatagtaa tcatctgttt 2820 tgtcaaacct ttcacactga ctcaacactc tacccttatt ttgtcttttc tgatttccct 2880 agcattgcaa gttttgcgtg taagataaga gtagtaaggc caaggccagg gaaagtcagc 2940 agtgtcctct tgcaacctta acataagaat gaatctccag acaaacactt gaaagtgctc 3000 gaaatcgatc gatcagagac aaaacagcga cacgaggggc ctggatgctt ttattatcaa 3060 tttttgcttt gcgtggcggt tccataaatc ccaattgcaa gtcccatcaa actttagaga 3120 tggaaagcgg ttgattcatt atattgcagt cgtcttcgtg gttaatctat caggtgatca 3180 cttccttaat ttcttatttt ctctcataat taaacagagg tacaacaaat tttctttttt 3240 gagtaaaaca gcaagttgat taaatggaaa aactttaaca ttttattcat tctcgatggg 3300 gagaaaagtc attctggctt agaatttttt tacatatata cagttgctat tgttatcaca 3360 tatgttcatc ttaatgtttt attgccaaca aagtttggta ttttagcaat ctacaagtat 3420 ttctgaaaat gtttttttag gtgtgtcatg gctttggttg aagctcacct tatttaaatt 3480 tttttttttg aaaataatct acgtttcatc tcgactaaaa ataatcacac attaaaatat 3540 ataagtgaga gagacaattc ttattcctta agttaatttt taggattaag ttagacttct 3600 cacattctaa gatcttatta atgtgagaga caactctcat tctctcactt actttattgt 3660 aagaatttta ttctttcttt atttttatga taagaaagtg ttaattttta atgtttattt 3720 tagagagaaa aaaagtttct attgtgaggc ccaggagaaa aataaattcc ttacctcccc 3780 aaattggcat gaaatgggtg aggggaaaaa tgatctctta ctaattttta ttttagtttt 3840 cttaatatat atatctttaa aaatagttaa atataaaaca attttatttt taaagataag 3900 tatatttttc tattagaatg aaaaatataa ctttttataa ggtgtttggc tatttggtgt 3960 ttccataagc atcgagaata acttaaactc tttatccttt ttctaaatat gcaaattaaa 4020 tcaaactcaa ggatcatcct ataccttttt tagttagcat gttattggta taaaatatct 4080 acttgtttta ccaataatca attttcatcc acaaatctta aatctaagac tttatttatt 4140 taaaggaatt gagtcaaata taacttcgac caacatcttg gaaatattta tttatttatc 4200 taagtaatta caagaaaaaa attctctgcg tttttgttat ttccatttat caaaataacg 4260 catctttgct actctattaa tttttttttc tttttttctt tcttattttt tctgtatttt 4320 atttctaaat caaatagaac attaatatgt ttctatctaa tcttttgtta cccataataa 4380 tcataaactt aaaaaaattt ctgtgtaaac taaacttgta gttttaaaac aaaaaataaa 4440 ttccatttgt ctactcaatt aataaataat tctaaattat ttaatattct ctcagtgttt 4500 atattttaac atctttaaat atcaaacact ctattaacac tcttaattta tctctatcat 4560 tcttttagta tctcattgtc tatcatatcg atgtttctct ctcttttatt tccatctctc 4620 aagatatcaa ttaaatttgg gtgtttgaag actttttacc atattaattt tcctactttc 4680 tttttatttt ttcatcttgc tcaatcattc gacttaactg acttgactac tttcttttta 4740 tatggtgagt atttaatgtg ttttatattt taaaaacatt aaatatttta tatttatttt 4800 tcatttaata cttttttcgt atggtaataa tattcttttc atttaatact ttttttgtac 4860 ggtaacactt ttttcaattt caaccaatac accttcacgt aaaaatatta aaaaaacaat 4920 taatttgata aataatttag aaaaagatat tagttaaaaa atacatgtaa tacttttaat 4980 caataccgat tatattttgt ctttatacta attaatcaat accgattata tttcctcttt 5040 aagtctttca ctataaattg aattcgagta atgttgtatg agaaatgcaa ggaagacatt 5100 ttttaacaaa ctcttgatga tttccttaaa aaaaatactt cttttataat tgattgaaat 5160 ttataggaaa tcataatttt ttggaatgct aggaacattc tctttggcac actcctttaa 5220 agattctttg tatatacgat tgactaaagt ttattgaaca attttgatag gttttaattt 5280 ttatttaata taaatcagga gagagtatga aaaataaaat gtaataaaaa tgatgatttt 5340 cattaaattc taaggaaata ttaaaaaaaa aagcgttaaa gaaagtgatg ttaacattta 5400 tggtattcac taccaagcac attaattagt aatcttttta atttgagttg tgatagttgt 5460 tttatccttg taaaaaaaat tctccaataa aaagtattag tatttatttt ttatataaga 5520 ttcaatttat aattttttat tttgattatt tttaaattaa attgcctcta aataagttat 5580 tatattggaa aaattaattg agatgagaga atttattata tgtcagaaat acaaatgtat 5640 ttaatattta cttctacaaa aaataattta aatacttaat tgaatatgtt ttttaggtac 5700 atccttaatt gaatatgttt aaaaatttct aagaaatttg agggtaatag ttaataagat 5760 gcatagtttt ctttttatta ttactgatag ttattaagta aaaatatcta tatttttcat 5820 atttttagat gctaaaagta ttggattctt aatatttatt attttaatac aatttaaaaa 5880 tttatagaaa gttaatatta gaaaaatata attaatttaa aattaagaat agcattgctc 5940 atttactatt ttactaaaaa tataaaatat tatctgtcaa gaaaatatta aatattaact 6000 atttacctta ggtagttatg aacattcaat gtattaaact tctaacgtat attatattga 6060 ttattgaagt tgttcaaaaa atattcttaa gggcactcgc tatagggcta cacaaactag 6120 cctacttctt gctttcccac atttttgctt ccgtcgggcg agttaaatgt tttagaattc 6180 ctggtttggc ag 6192 <210> 21 <211> 1999 <212> DNA <213> Zea mays <400> 21 tcggcctgtc cattattgta ggaatagcta gccgcccatg caagttctat tcagcctgcc 60 ttttcatcgc tctgaccacc atgtcctcat ctgcgtgaga tgtgctatca atagttggac 120 acaattcgtc ctcaagctac gacggggaca gtttgacatt accagagaga gagagagaga 180 gagagagaga tagagtgcta gcaataggag gggatgcaaa aacatgtacc acctcaccgt 240 tctgtcgtac ttccctctga tcaacttcac aacgttcaaa tttaggcaag gcgctgtggc 300 tgaagtttac aatattctgc accacctcac tgtcttttgt agttccttct gatcagcttt 360 aagttgttcc aattcaggca aggcgctgtg ggttaagttg acaattgtta cctcagcttg 420 atcaacagat gacacttttt ttttttggtg aagaagtgtt ggcttccggg tcacaagagt 480 atcctctcct ctgtactgtt tctgaaactt gttgccttcc tgtccattgt ggcgattagg 540 ccggggttaa aaattcccag tttcgataac gattgctcgt tgctgggtta gactttgcac 600 agaaatcttc cgctcgagga gggggtttag ttattaatta cccacagtga tgagagtgac 660 gtacgctgta ctagaatttt tgcgaatgac tgacagtaat gacatcatcg tccctgacaa 720 taatatccaa catccaacgc ccccacactt atattccagt ccaggcatat tgtatatgaa 780 cgagcgagca cgcacggtac gtacgtgtgc ggtgtgtccg ccggcgccta ccaggacgta 840 gtacaacatc gccatctgag cccatgacgc ccgcccagcg ccagcgccag cggcacggca 900 aaagtcgccg gcgccagcaa gaacagcagg aagcacaggt ttcaaagggg gcgccgcgag 960 ccagggccca ggaccagcag cacgtacaga gcgggagtaa taaacgctga aaagctccgc 1020 gccctctcgc gcaatcaatg gcgacgcgac gcgaactcta ccggccggct ccgtccgccc 1080 cagtcggggt ccccacgccc ccagggggag ggtgcctctc tcgctcactc acccgtcgcc 1140 ctgcgtgccg aattaatggc ggcgaccgct gcgtgcaact gatccgaccc gacccgacca 1200 gaccccccgc gccgacgacc gcctagctct gcactcaccg ggaatcagag accgaggcgc 1260 cgcgagtagc agaggcaacc acccgttctt tggagtcgga gcgtcccggc ccggtcggga 1320 tccattttct gcacgtcgtc accactcacc acgcgcattg gaaccgccgg aagccgcgcg 1380 tgtgaaaaag ctgctgcttc atggtcatgg tcatggcggt cacacagcag cttaatttgt 1440 tacgctaagt cgctaacagt tgagacactg gcacaagcag catggccggc ctccggctct 1500 catgagacta cttgcctcct gtgccagtgc cagccagtat agaaattgag acgacgacgt 1560 cgacggcaac aacagccgtc accaccacgc cgcgtctcaa tcccgttggg tttcaggcgc 1620 cataactcca gcagtcccgc tgcgagtgag ggggagccgg tggcacggct gggctgcaac 1680 gatccgtgtc cccccgcgtc ctcctccttc ctactgtgcg gtgccggtgc cgctgccccc 1740 aattgctcgc gttgcttgcg tcccttggcg accaagttac atgtataaag ccatgcatga 1800 ccagagaaga acacacaact gtcctggcct gtccacggtc cacacacgcc ttccttatat 1860 cggcgctcac ggttctataa aaaaaacaca gtacgagcag agcaaggcgg ccgtgagctg 1920 agctgaggga aggacgacgt acggctcagt tgctagcccg gccgccggag ctctccgaga 1980 tctgggccgc cagcaacac 1999 <210> 22 <211> 3000 <212> DNA <213> Arabidopsis thaliana <400> 22 attttaagaa attaaatctt tttttttggt atctgtgtgt tactttttat aaatgtattt 60 tgaaaagtat tatttcaatt tatagataaa tataattatg actctcaagt ggattttttt 120 ttcacggtca attggagttt ggctgtgaat cttgtgaata atatcaacta attaagagtc 180 ggccgatact ccgtatcttt gatcactttc acccatatct tgaaatttac caaatgtttt 240 ctttcattgg ggtttctata cctttttttt ttggttcttt ctgtaatttg ggttttaaag 300 aaaaaaccta taattacaaa aaaggttaaa agttaaaact aagcacgttc aaatatgttc 360 tttcgagatc ccaccttcct gtctatctta taagaagtcg aacacgtgtg ctcgttgtgt 420 gaattagagt ggttatttca acatatataa ttaaactatg tatgtatcat aaatacactg 480 aatatgttgg agttgggtct tgtggtaggc taacttgttc gcttcacttt ggtttagttt 540 agccttgtaa tcggtggttt catatactga aatttttaat tttctaatat aatccagatg 600 acaaaaaaaa atcataaata cactgcaatg gccattttta aaaaaacttt aaacgaaata 660 tatatacatc accaagtatc aagtaagtaa tttggttgtt ttgatttttc aaaaaaacat 720 ttaggtgatt cttttaacaa ccaatcatat atgtagcaaa gattaattat gtcaatcttt 780 taacgtcact tatatagttt agctgtagta ttattgtaag ccaattgtac ggacttcgta 840 accgtttcga caaagataca aactaatcaa tgtttaagat ggcataatat tccgacttcc 900 cctgccactc ggtagtactg ctgcgccatc ctacaagaag cactcgtaaa tgagtggtta 960 gacggccaaa aattagcagg attcaatcct tgattaagag atggttcaga taagccctct 1020 aggcctctac tcgtcacaat tcatatatta attgatcaca ctgcatgaaa tgataagatc 1080 aagtcaatta cctcaaaatt aattgaaaga taaaatgata aagcaacaag taatgatcat 1140 atatggatgt aacaaccttc ttcatccata cgaatatgat aattcttttg tcgatcattt 1200 gccacatcta tttaactaag tattttgtac attaagatca ttttgcgagt attcccgcgt 1260 tcccaacaaa aacgaattga aaaactacat agggtgaatt ttgctctcgt ggcattaaag 1320 agagaggctg acattataga ttatgatata tttattattt ttcttagtag atttcttgac 1380 cagcctttag atatgcttgc tctgtcatgg cataaagcct taaaccctaa agccctttac 1440 atttgtacag taccaacaat tgtacccaat gttaaatgaa tgcggattaa tttgtgatat 1500 gagaccatca aaataaaata cttataagat agtagatata tagaaccgta acaaaaagat 1560 gagaagaaaa agacaaattg aatagaaact ggaccaaaag ataaaagagt atatgaagaa 1620 gcaagccacc aagaagtgta accatcgttg cttcaactgt aaaccgcaca ttaaatgtgt 1680 gtgagagaaa gagagataga ctccagctgt gagagctacg tccattgcat caattgggtt 1740 atatgtcgtt aacaattttt taaaaataca taaataaatc aataaaatat ttagaaaaaa 1800 tcaatattat aatgatattt tgaagaagtt aggtccataa tggaaatata gagacttagt 1860 gtctatttat caagaaatag tgtctattta tcaagaaata atgtctattt ggtccacaat 1920 tatcatattt tttgtggcca ctcaaactac gaaaataatg aatattgtta atttttgtct 1980 tatttaagtc tataactaac ttactaattc ggttgaaatt gctaatatgc attttccttg 2040 gtccactatt acagtgctac tgctgtctta ttcttgaaca caaaataaaa aagatttcaa 2100 acacaaaata tgcatttttt tgttccacca ttacaaacct ttatttggag aagtatacag 2160 ttggatatta aatattttaa ttaaaaaatt gaaaatattg aagttctttt tctccagtat 2220 tttggctgga gcaaaactaa ataaattcca aattaaaagc tcgaacggaa aacatttact 2280 atgcaccaaa ataaatgttg atatgaaatt tgcatcagca tatacagtat gtatgtagta 2340 caattgaaca gagtcaaaat taattgtcac tgaaacagtc agattttatt tctgttaaaa 2400 aatattttca gacaaaaaat gattttttaa cgttttttta tataatttta ttaaacctca 2460 aaacattcct cagcagattg ttttataatg ttattattat tttttcttaa ataattttat 2520 ttgaatttcg ttatagagag ataaataaaa gatttttggg gtctagttgg ctctcatagt 2580 ttgaatgctt atataaggaa acagagctcg aggctctcaa taatgccaca taaaacacaa 2640 caccaaagcc tttataactc tttctggggt ttttaattct ctcaacgaac aaaaaaaaaa 2700 actcaactaa tattctccat cctctttctt cagatttaag agaaacctta tcgttatatt 2760 ctctcttctc ttttcttttt gactcccaca agaagaaagc aaatttcttt ttctcaggat 2820 tgcttgaaaa ctgtagaaat ctttaaggtt tttttacacc aataagagtc agagagagag 2880 agagagagag aaagagagat ctattcataa aaaagacagg taatctcaac tccgaggaga 2940 taactgatat aacaatttga gagaataaga agaagaagaa gaagaagacc aagctaaaga 3000 <210> 23 <211> 2620 <212> DNA <213> Arabidopsis thaliana <400> 23 attttaagaa attaaatctt tttttttggt atctgtgtgt tactttttat aaatgtattt 60 tgaaaagtat tatttcaatt tatagataaa tataattatg actctcaagt ggattttttt 120 ttcacggtca attggagttt ggctgtgaat cttgtgaata atatcaacta attaagagtc 180 ggccgatact ccgtatcttt gatcactttc acccatatct tgaaatttac caaatgtttt 240 ctttcattgg ggtttctata cctttttttt ttggttcttt ctgtaatttg ggttttaaag 300 aaaaaaccta taattacaaa aaaggttaaa agttaaaact aagcacgttc aaatatgttc 360 tttcgagatc ccaccttcct gtctatctta taagaagtcg aacacgtgtg ctcgttgtgt 420 gaattagagt ggttatttca acatatataa ttaaactatg tatgtatcat aaatacactg 480 aatatgttgg agttgggtct tgtggtaggc taacttgttc gcttcacttt ggtttagttt 540 agccttgtaa tcggtggttt catatactga aatttttaat tttctaatat aatccagatg 600 acaaaaaaaa atcataaata cactgcaatg gccattttta aaaaaacttt aaacgaaata 660 tatatacatc accaagtatc aagtaagtaa tttggttgtt ttgatttttc aaaaaaacat 720 ttaggtgatt cttttaacaa ccaatcatat atgtagcaaa gattaattat gtcaatcttt 780 taacgtcact tatatagttt agctgtagta ttattgtaag ccaattgtac ggacttcgta 840 accgtttcga caaagataca aactaatcaa tgtttaagat ggcataatat tccgacttcc 900 cctgccactc ggtagtactg ctgcgccatc ctacaagaag cactcgtaaa tgagtggtta 960 gacggccaaa aattagcagg attcaatcct tgattaagag atggttcaga taagccctct 1020 aggcctctac tcgtcacaat tcatatatta attgatcaca ctgcatgaaa tgataagatc 1080 aagtcaatta cctcaaaatt aattgaaaga taaaatgata aagcaacaag taatgatcat 1140 atatggatgt aacaaccttc ttcatccata cgaatatgat aattcttttg tcgatcattt 1200 gccacatcta tttaactaag tattttgtac attaagatca ttttgcgagt attcccgcgt 1260 tcccaacaaa aacgaattga aaaactacat agggtgaatt ttgctctcgt ggcattaaag 1320 agagaggctg acattataga ttatgatata tttattattt ttcttagtag atttcttgac 1380 cagcctttag atatgcttgc tctgtcatgg cataaagcct taaaccctaa agccctttac 1440 atttgtacag taccaacaat tgtacccaat gttaaatgaa tgcggattaa tttgtgatat 1500 gagaccatca aaataaaata cttataagat agtagatata tagaaccgta acaaaaagat 1560 gagaagaaaa agacaaattg aatagaaact ggaccaaaag ataaaagagt atatgaagaa 1620 gcaagccacc aagaagtgta accatcgttg cttcaactgt aaaccgcaca ttaaatgtgt 1680 gtgagagaaa gagagataga ctccagctgt gagagctacg tccattgcat caattgggtt 1740 atatgtcgtt aacaattttt taaaaataca taaataaatc aataaaatat ttagaaaaaa 1800 tcaatattat aatgatattt tgaagaagtt aggtccataa tggaaatata gagacttagt 1860 gtctatttat caagaaatag tgtctattta tcaagaaata atgtctattt ggtccacaat 1920 tatcatattt tttgtggcca ctcaaactac gaaaataatg aatattgtta atttttgtct 1980 tatttaagtc tataactaac ttactaattc ggttgaaatt gctaatatgc attttccttg 2040 gtccactatt acagtgctac tgctgtctta ttcttgaaca caaaataaaa aagatttcaa 2100 acacaaaata tgcatttttt tgttccacca ttacaaacct ttatttggag aagtatacag 2160 ttggatatta aatattttaa ttaaaaaatt gaaaatattg aagttctttt tctccagtat 2220 tttggctgga gcaaaactaa ataaattcca aattaaaagc tcgaacggaa aacatttact 2280 atgcaccaaa ataaatgttg atatgaaatt tgcatcagca tatacagtat gtatgtagta 2340 caattgaaca gagtcaaaat taattgtcac tgaaacagtc agattttatt tctgttaaaa 2400 aatattttca gacaaaaaat gattttttaa cgttttttta tataatttta ttaaacctca 2460 aaacattcct cagcagattg ttttataatg ttattattat tttttcttaa ataattttat 2520 ttgaatttcg ttatagagag ataaataaaa gatttttggg gtctagttgg ctctcatagt 2580 ttgaatgctt atataaggaa acagagctcg aggctctcaa 2620 <210> 24 <211> 2792 <212> DNA <213> Glycine max <400> 24 ttaaaaaaaa tctcaaaaga ataaattttc gactaccagt aattggattt taaataggaa 60 gagattaaaa aaccacctct taatcttata ttccatcttc cattccataa gattgtgggt 120 gtcattaaaa cttattatca atacaaaatg aaaaataaag agtcacggtt tgttgaggag 180 aagaatgcac ttagttccaa atcccaatac atgtattaag tagtttcgtt aacttttttt 240 tattaaattt taattatgtt caattaatta tcaatttcta taattaattt attattttac 300 cccgaatttg agggggaaaa agttaaactt ataagatact ttccatgttt tttaaaaatt 360 gcattcaata tttttctttt ttttttcacc ctaaaaaaat attttaatta gttaactcac 420 attacactca gatcccctta acagaaggtc catgttgatc acattatttt taataaaaaa 480 ttatgtctaa aataccctca aataaattac acttgcactt ccatgtgttt ttttaaaatt 540 acatctaata ttcctccttt tttgtacact ataaaaaatc tcttaattat ttagctccca 600 ttacattcac taagacttct tccctctcct aaacacagtt taataattta atagaaagta 660 gacacaattt gatcatatga cttttaatga aaatttatat ctaaaatatt cttatcttct 720 tcctcttaac ttcataaaaa acataacatt attttgaacc tgaaaatatt ttaatacttt 780 tttttctcta attgaacctg aactcacctt gttggtacgt atgtgttaat atctttcctt 840 tttttttttt acttcaattt tttttgtgtg ttgagttgtt tggttgcaac atttttgttg 900 gacatgttcc caacaatgcc aattgattga atatgatcat ttgcttcaaa ttttaatgtt 960 ttgttcatgc atttgtgttt taactccttt ctttgaaata tttatttttg atgactagtt 1020 gcttgatata atgtctcaat caagagttta tatcttcttc gagtaatagc aacaacgaag 1080 taaaaataaa atattaaagg aaaattgtta ttgtcttaag aaaaattcat taacagagtc 1140 aattgttaat ttgttattga gtatttaaca aagatgttag gacaatttca tctgctcata 1200 tattccaatt gatgttaatt aacgacgtta acggaaagag ataaaaataa tgtaaaaaaa 1260 ggaaggtata tcagatgcaa tttgagaaaa acacaagaaa tacgattata acaaaaatgt 1320 taaaacaatt ttatctgttc acatatttca attaacgtaa caacgttaat aaaaaaggga 1380 taaaagtaat ataaaaaaga gaagtatcaa gtgtaatttg aaaaataaaa aaacacaaga 1440 aatacgagta gtgtaatttt ttttagatta aattaaataa taaagtcttt gttaaattaa 1500 acataaggtt actttataat gctctcataa tgaaaaccgg atttagaggt aactccaaac 1560 ctaccttatc taacagctga gaggtactgt ttcacctgcc aaccatataa ggcatttact 1620 actactctcc agatctctta ctgctggatt acaacacgct acagcaacac gacgcgcggg 1680 gtgtgtgttt tcgtttacgt agattttaat tttttaaggc tctaattata attaattaca 1740 gcgtaattaa tatggagtac tataaagtat aataattata ataagccatg tatataaaaa 1800 ggagaagtgt atctgttaat gtgtactaat atcttacaca aaagtatctg ataggagtgc 1860 tcgtgaaatg aaataaaatg atgttattac cagaataaca aatatagaat ttagctaaca 1920 tatatagtta ggttaaaatg tttttatata aatatttaat agaaaattac attctcctta 1980 cattatagtt agcattaacg attaagatga tataataaaa tgatcaaatc ttattaaata 2040 tctgtctaaa aataacttaa actaatataa tcggtatata aacttaactc ttaaatacaa 2100 cagtgattaa aataaaatca actttatcaa ttattatatt taaaaaatta aatagaaata 2160 attttatgaa aaaaattact ataaaatgca ttgataatat tgaatactaa taatttttaa 2220 tagtttgttt ttagaccacc aaaccagaat caatcaaggg agggttattt cagcttaact 2280 aagtttttat atattatttt atttatttca taagattaaa atctcaagtg agatacatga 2340 atcaaatagt agtacttttt tttagaatat attaattgat aatttaatgt cccagttagc 2400 agaagacagg tgggagacta tcttactttg taggccacaa ggatcaatgc atgatccgca 2460 gcgcttataa atacgtgatg ggacgaaaga atatattttt aagttgtggc tcaaaagcaa 2520 ttactactaa gaaaggataa aggaaggaag gaacactttt ctgatattct ttttggtatt 2580 gggttgctga cctttgctag tgttatagat tttttgggtt ctcttgtgtt ttaataaata 2640 caggaaaaaa aaagtatata ggtgcctaga agaaaagaca atatagggtg tggagcaatt 2700 acgtttgatc acaagggaaa aggtgcaacg gtttgcaaga tagcatagaa gattttgaag 2760 aatcttaatt gcaacatagt agtgcagttg ca 2792 <210> 25 <211> 2500 <212> DNA <213> Glycine max <400> 25 ttaaaaaaaa tctcaaaaga ataaattttc gactaccagt aattggattt taaataggaa 60 gagattaaaa aaccacctct taatcttata ttccatcttc cattccataa gattgtgggt 120 gtcattaaaa cttattatca atacaaaatg aaaaataaag agtcacggtt tgttgaggag 180 aagaatgcac ttagttccaa atcccaatac atgtattaag tagtttcgtt aacttttttt 240 tattaaattt taattatgtt caattaatta tcaatttcta taattaattt attattttac 300 cccgaatttg agggggaaaa agttaaactt ataagatact ttccatgttt tttaaaaatt 360 gcattcaata tttttctttt ttttttcacc ctaaaaaaat attttaatta gttaactcac 420 attacactca gatcccctta acagaaggtc catgttgatc acattatttt taataaaaaa 480 ttatgtctaa aataccctca aataaattac acttgcactt ccatgtgttt ttttaaaatt 540 acatctaata ttcctccttt tttgtacact ataaaaaatc tcttaattat ttagctccca 600 ttacattcac taagacttct tccctctcct aaacacagtt taataattta atagaaagta 660 gacacaattt gatcatatga cttttaatga aaatttatat ctaaaatatt cttatcttct 720 tcctcttaac ttcataaaaa acataacatt attttgaacc tgaaaatatt ttaatacttt 780 tttttctcta attgaacctg aactcacctt gttggtacgt atgtgttaat atctttcctt 840 tttttttttt acttcaattt tttttgtgtg ttgagttgtt tggttgcaac atttttgttg 900 gacatgttcc caacaatgcc aattgattga atatgatcat ttgcttcaaa ttttaatgtt 960 ttgttcatgc atttgtgttt taactccttt ctttgaaata tttatttttg atgactagtt 1020 gcttgatata atgtctcaat caagagttta tatcttcttc gagtaatagc aacaacgaag 1080 taaaaataaa atattaaagg aaaattgtta ttgtcttaag aaaaattcat taacagagtc 1140 aattgttaat ttgttattga gtatttaaca aagatgttag gacaatttca tctgctcata 1200 tattccaatt gatgttaatt aacgacgtta acggaaagag ataaaaataa tgtaaaaaaa 1260 ggaaggtata tcagatgcaa tttgagaaaa acacaagaaa tacgattata acaaaaatgt 1320 taaaacaatt ttatctgttc acatatttca attaacgtaa caacgttaat aaaaaaggga 1380 taaaagtaat ataaaaaaga gaagtatcaa gtgtaatttg aaaaataaaa aaacacaaga 1440 aatacgagta gtgtaatttt ttttagatta aattaaataa taaagtcttt gttaaattaa 1500 acataaggtt actttataat gctctcataa tgaaaaccgg atttagaggt aactccaaac 1560 ctaccttatc taacagctga gaggtactgt ttcacctgcc aaccatataa ggcatttact 1620 actactctcc agatctctta ctgctggatt acaacacgct acagcaacac gacgcgcggg 1680 gtgtgtgttt tcgtttacgt agattttaat tttttaaggc tctaattata attaattaca 1740 gcgtaattaa tatggagtac tataaagtat aataattata ataagccatg tatataaaaa 1800 ggagaagtgt atctgttaat gtgtactaat atcttacaca aaagtatctg ataggagtgc 1860 tcgtgaaatg aaataaaatg atgttattac cagaataaca aatatagaat ttagctaaca 1920 tatatagtta ggttaaaatg tttttatata aatatttaat agaaaattac attctcctta 1980 cattatagtt agcattaacg attaagatga tataataaaa tgatcaaatc ttattaaata 2040 tctgtctaaa aataacttaa actaatataa tcggtatata aacttaactc ttaaatacaa 2100 cagtgattaa aataaaatca actttatcaa ttattatatt taaaaaatta aatagaaata 2160 attttatgaa aaaaattact ataaaatgca ttgataatat tgaatactaa taatttttaa 2220 tagtttgttt ttagaccacc aaaccagaat caatcaaggg agggttattt cagcttaact 2280 aagtttttat atattatttt atttatttca taagattaaa atctcaagtg agatacatga 2340 atcaaatagt agtacttttt tttagaatat attaattgat aatttaatgt cccagttagc 2400 agaagacagg tgggagacta tcttactttg taggccacaa ggatcaatgc atgatccgca 2460 gcgcttataa atacgtgatg ggacgaaaga atatattttt 2500 <210> 26 <211> 2691 <212> DNA <213> Oryza sativa <400> 26 tgccggttcg gcggaagaaa caccgaccaa attttattaa gattgaaaga agttacatgc 60 atccgacatt agaccgtcga gttaaggcga gaagacctaa gaagcaaaac aaaagaaaaa 120 ctgagagaag aatgtaaagg gcaagtgcac ttagggctac tcctcactaa accttctttt 180 gtggattgat gcatcaaaaa actatatgta ctaggttaca tcgtgttttt tttttcgttt 240 tcgtctcatt ctctgcttcg gcctgccgtc tgcttatacc gttaattcga tgatgactcc 300 agacttcatt catgaatata tttctactat agatatacaa ccctcgctcc tctatgttta 360 tcttcatctt catcttcagg agattcaaac accacctctt aaagatgaag atgaagatga 420 agataaaaat taagtgtttc acgtaaaacg atgtattaat aacatgtgat taatagagtt 480 ttaattgtta taaacttgaa aaatgaatta gtatgatatt ttagagcaac tttcatatat 540 aaagttttcg cacgagacgt accgtttagc agtttgaaaa gcatgtcacg agtatccaaa 600 atttaatctt gtgcttgcag aagaaacgaa gagggcctaa cccatataac ctgcgtcatt 660 aatctatagc ttagcaaagt ttactttttt gcctgcctcc tggcacagga acgccgacga 720 agaaaaacct acaggctttc tcaactatga ttccttcctc aacctgtgaa ctctggcgac 780 aggaggacga caggggtgta tgtggagcag ataatgcaca gtaataatcc aatccttagg 840 acttacaaac tgctggaatg aactgaccaa gtacgtttag aacgtaggaa ttttgactta 900 tacatgcata gtgtgatttg tacgtgaaat acattaatta attttctgaa tttgtttcca 960 atttctcgtc gtcaggcaat gccgcaatgc cttgtcttaa agattgcaaa ttgaatgcgc 1020 catctccatc gatctctcta tctccattga tggctgccca aaaaataaaa aataaaaaga 1080 gccgacaatg tttaaggaga gaagcatacg cgcacgtttc gtgatgaaac atacatgcaa 1140 gactaatttc gaattatata tcacatattc gctctctgaa tgagcgtatc gggtatttgg 1200 aatttcgttt gatatgtaaa agttgaattt taggttactt ttttgtggaa taatatatca 1260 taaattaata ttttttataa ctatttatgt gacatgtaaa tgacgagctg atatcagttt 1320 aggataattt cctcgctagg tgattgaacg aaccctgcac gcggcagacg tcgtggctac 1380 tactatctag ctagctagcg ttggttacgc gtggctacta tccagccaga attggttacg 1440 ccgtcgccca tcgatccgcc acaccacgcc aaacgcgaac ttaattgcta ctatccgata 1500 gtatgtacgc gtactacaca aaagtgggga aaagaaggtt gtcctgactc catgagaaga 1560 cgatccaaaa cacggggccg gacaaccaaa caagctagct agcggccggc ggcgagccac 1620 cgtcgtcgtt gctgaagacg tcgactaatt tggcctcccg gccggcgcca tcgatcatct 1680 caaaattaat actccctctg tataagggat tttgagtttt tgtttgcact gtttgatcac 1740 tcgttttatt aaaaaaattt gtgacaatat aaaaaacgag aagttgtgct taaaatactt 1800 cggataataa agtaagtaaa aaataaataa taattctaaa aaaaatttaa taaaataagt 1860 gtgcaaacaa tacaagcaaa aactcaaaat cacttataat catgggacgg agggagtata 1920 tagtatccaa aaatcatctc aaaattaaaa ccgaatcgaa actcgccggg ccgcggcccg 1980 cccggccgtc ctggcccaat taattccggc gaccgctgca tgcaacgctc cgtacgtgat 2040 cgcccagcgg ctcgcgcgcg caccacgcgt ccacgcgtct ctctccttta tacatggaca 2100 atggattaat ttacttgtag aaaaaaaaac acgaggctag cttagcttgt gcccggccca 2160 gccgcgtgcg tgatttcccc aagttcacaa cctgtacgtg tgtgtggtac gtagcggtcg 2220 caatcccgtt tcaaatctcg atcgtgtgtg gtgccattaa ctccgtctgg ttggggaagc 2280 cggtggcgga tagcccgact gacaggtgct gtggcctggc ggccggccgg ccaatcgatg 2340 gatggacgga tggatcccat cgatgatcct tcgttgattc gcgtcaacct ctcgctgccc 2400 gaatcggact ctcaccttcc ccgccctcca ccgctgtgac cgcgcgctgt ctgtcccttg 2460 gctaatacca gtcgcagtgc aactgtgcaa gtgtttcacg tatataaaag catcccgaga 2520 cgcagcgcgc atgaccagcc gcgttgggtg caaaaccaca accgccaaca tcttctaact 2580 cccctcacgg ctctccctct ccccgcacgc acaacaccgg tgaaccccta cgtactactg 2640 agctgagctg agaggacgaa gaagaagatc aagctcgagg ggcttgcgat c 2691 <210> 27 <211> 2500 <212> DNA <213> Oryza sativa <400> 27 tgccggttcg gcggaagaaa caccgaccaa attttattaa gattgaaaga agttacatgc 60 atccgacatt agaccgtcga gttaaggcga gaagacctaa gaagcaaaac aaaagaaaaa 120 ctgagagaag aatgtaaagg gcaagtgcac ttagggctac tcctcactaa accttctttt 180 gtggattgat gcatcaaaaa actatatgta ctaggttaca tcgtgttttt tttttcgttt 240 tcgtctcatt ctctgcttcg gcctgccgtc tgcttatacc gttaattcga tgatgactcc 300 agacttcatt catgaatata tttctactat agatatacaa ccctcgctcc tctatgttta 360 tcttcatctt catcttcagg agattcaaac accacctctt aaagatgaag atgaagatga 420 agataaaaat taagtgtttc acgtaaaacg atgtattaat aacatgtgat taatagagtt 480 ttaattgtta taaacttgaa aaatgaatta gtatgatatt ttagagcaac tttcatatat 540 aaagttttcg cacgagacgt accgtttagc agtttgaaaa gcatgtcacg agtatccaaa 600 atttaatctt gtgcttgcag aagaaacgaa gagggcctaa cccatataac ctgcgtcatt 660 aatctatagc ttagcaaagt ttactttttt gcctgcctcc tggcacagga acgccgacga 720 agaaaaacct acaggctttc tcaactatga ttccttcctc aacctgtgaa ctctggcgac 780 aggaggacga caggggtgta tgtggagcag ataatgcaca gtaataatcc aatccttagg 840 acttacaaac tgctggaatg aactgaccaa gtacgtttag aacgtaggaa ttttgactta 900 tacatgcata gtgtgatttg tacgtgaaat acattaatta attttctgaa tttgtttcca 960 atttctcgtc gtcaggcaat gccgcaatgc cttgtcttaa agattgcaaa ttgaatgcgc 1020 catctccatc gatctctcta tctccattga tggctgccca aaaaataaaa aataaaaaga 1080 gccgacaatg tttaaggaga gaagcatacg cgcacgtttc gtgatgaaac atacatgcaa 1140 gactaatttc gaattatata tcacatattc gctctctgaa tgagcgtatc gggtatttgg 1200 aatttcgttt gatatgtaaa agttgaattt taggttactt ttttgtggaa taatatatca 1260 taaattaata ttttttataa ctatttatgt gacatgtaaa tgacgagctg atatcagttt 1320 aggataattt cctcgctagg tgattgaacg aaccctgcac gcggcagacg tcgtggctac 1380 tactatctag ctagctagcg ttggttacgc gtggctacta tccagccaga attggttacg 1440 ccgtcgccca tcgatccgcc acaccacgcc aaacgcgaac ttaattgcta ctatccgata 1500 gtatgtacgc gtactacaca aaagtgggga aaagaaggtt gtcctgactc catgagaaga 1560 cgatccaaaa cacggggccg gacaaccaaa caagctagct agcggccggc ggcgagccac 1620 cgtcgtcgtt gctgaagacg tcgactaatt tggcctcccg gccggcgcca tcgatcatct 1680 caaaattaat actccctctg tataagggat tttgagtttt tgtttgcact gtttgatcac 1740 tcgttttatt aaaaaaattt gtgacaatat aaaaaacgag aagttgtgct taaaatactt 1800 cggataataa agtaagtaaa aaataaataa taattctaaa aaaaatttaa taaaataagt 1860 gtgcaaacaa tacaagcaaa aactcaaaat cacttataat catgggacgg agggagtata 1920 tagtatccaa aaatcatctc aaaattaaaa ccgaatcgaa actcgccggg ccgcggcccg 1980 cccggccgtc ctggcccaat taattccggc gaccgctgca tgcaacgctc cgtacgtgat 2040 cgcccagcgg ctcgcgcgcg caccacgcgt ccacgcgtct ctctccttta tacatggaca 2100 atggattaat ttacttgtag aaaaaaaaac acgaggctag cttagcttgt gcccggccca 2160 gccgcgtgcg tgatttcccc aagttcacaa cctgtacgtg tgtgtggtac gtagcggtcg 2220 caatcccgtt tcaaatctcg atcgtgtgtg gtgccattaa ctccgtctgg ttggggaagc 2280 cggtggcgga tagcccgact gacaggtgct gtggcctggc ggccggccgg ccaatcgatg 2340 gatggacgga tggatcccat cgatgatcct tcgttgattc gcgtcaacct ctcgctgccc 2400 gaatcggact ctcaccttcc ccgccctcca ccgctgtgac cgcgcgctgt ctgtcccttg 2460 gctaatacca gtcgcagtgc aactgtgcaa gtgtttcacg 2500 <210> 28 <211> 2946 <212> DNA <213> Populus trichocarpa <400> 28 tactgtgaaa aaataacttg tccagttttt atttcatctg catttttcaa taccaacaaa 60 atcatcgtga aaatatttga tctaaaaaat cattttttac catctatttc ctattagaaa 120 ttccaacacc atttattttt atgatagaaa attttattta tttatttgat aaaaaataat 180 gaaagaaaat gacgaggaac ttaataaaaa aatataataa aacaagaaaa aggataagat 240 ttttttaaaa gaatgacaac taagattgga taaaaaaata aaataaaata ctgagggaca 300 aaattgaaag aaaaaacaaa ttaagaaaag gataaaaaag agcaattcca aaaataagga 360 ctaaatctgg aaaaaaagaa atgaaataaa atatcgaggg acaaaattga aagaaaaaca 420 aaccaagaaa attataaaaa aaaacaacaa tcaagagaat gagtaccatg tttgatataa 480 taacaaaaca aaatcaatga aaatggatga aaatgaagaa aaaatccaaa acaaagaaag 540 agcaattaaa agaaagagga tcataattga atttttttca ccattatttt ttttatcaaa 600 ataatataaa ttttactttt tagttgaata aaaaatcaag ttattagtta ggggaaaatc 660 atgttaacac ccattgattt ttatttttgc cattattttt ttttatcaaa ataatgtcat 720 tttacttcct gcaaaataaa aaactcgatt gaccctggta accctgatca ctcgacccaa 780 cccatgactc gggagatgat gggggtcgac tcctaagccg ggttttaaaa gcatgataaa 840 aaaataagca ctaaatatga aatgaaaatc caataaaatt aaacaatgac tgatgaaatt 900 gcgaaaaaaa attaatcaat aagagaacta aaaataacag gaattacaat cataagaata 960 atgaacgaat tcaacaaaaa aaagaattaa aatcaaatga taatggatta aattgaaaaa 1020 taaattccaa ttagaaaaaa aaagattaaa agcaaaataa ataggaataa aaaaatgagg 1080 atcaaatttg acaaaaaaca taaaaaaatc aaatgttaag ggatgaaatt aaaaaataaa 1140 taaatcagat tcgatacaat tagaaaattg agaaccattt tgcaacttta caaggttaac 1200 acatcttttg atatagagga gagagaaaag aggggtggag aaaatttttt gcttgaactc 1260 cgctgcgcca cgaatgatga catgtgttgg cccaccgtgt ggagggcgac atgacacttc 1320 ggatgccgtt gttaaagacc actaacaacc actgggagga gcctcacgcg ccactcaaaa 1380 ggcgcgagcg ccttccactt gctggcgagt gctctgcgcg ccccaactac tttttttttt 1440 gaattacaga aagatcataa cattcatgac cccacatgat aatttaaaaa aaatctaaaa 1500 acaccccttg accttaaaca agaaaaagtt aattccaagg gttattacag agagcaaaac 1560 actcctaatc ccatgtgata attgcaaaaa taaacctagg tttgagaaac caaagatacc 1620 ctttaccctt agaaaatatt tttgacccca agagctattt ggtaattaga ctatgaaaaa 1680 atatgaaaat taagaaatga ccattgaaca taaaaccttt ttttttcctt ctaaaatcat 1740 gggcgtaatt atactaagca acaaaatgta aaaatactat tccactctta tatgtatttt 1800 atattttgat atctttgaag gacaaattaa tcgttttatt atgaattgca ctgtggacaa 1860 tccacaataa aatactgata ccttttagtt atatgttaaa cattgaagaa atgttttttc 1920 atatttttca cgattttttt atttttatat cttttagttt tccaaattta atagtaaaaa 1980 ttaaattggg taaaattatt agtctaaata tgatgtaaac ataaacatta aaatgataaa 2040 atatttaaag agatatttat ataaaaaaat taatttattg ccatccctat cttttatctt 2100 ctttaacctt ccttgctaga aagaataaaa aaaaatagtt aaaaaatatt cttaaaatca 2160 agacaaaaat taattattat ttccatctcc accataaaat atatttctta ttggtttatt 2220 tgatattata atagcggttg tttttttata aatatattaa ttatttttta aaatttattt 2280 ttaacattaa cacatcaaaa taattttaaa aaattaaaaa attaatttta aataaaaaaa 2340 ccctataata gaacgtaaaa aaaacaaaca cgtattttga attaattacc aatttggcac 2400 aaaccgtgag ctgaattctt caactgccca accaaagagt cattaatttc taggagcatg 2460 aggccaccac tccagctgtg tgccaaatga caagggagga gaggaaatga gacaaaaaca 2520 tggaagtgtg agctgctaaa taaagattga ttaagaactt caactgtatt tttggtcctt 2580 ccttcaagct atatatatgt gggtgcctcc ataaagatat cgcagttacg agtaatagat 2640 atacagtgtt agtcgagtct ttaaaggaaa aaatcaaaga gagagagact gaacttgata 2700 ctctcttcct ttcatctctc tctccccctc ttaccctcta agggttgctc catctggagc 2760 ttgcgttcat gttttttcgc ttcatttgtg gtgtagctaa ccactttgag ccataaccag 2820 ggaactatct ttttacccaa caagataaag ctgcttacag gagcaaaatt gagacatata 2880 ctccagtaaa aactcaagaa aaaattgaag aaaaccgttg aagatcaggt gctttactgc 2940 aaaaaa 2946 <210> 29 <211> 2500 <212> DNA <213> Populus trichocarpa <400> 29 tactgtgaaa aaataacttg tccagttttt atttcatctg catttttcaa taccaacaaa 60 atcatcgtga aaatatttga tctaaaaaat cattttttac catctatttc ctattagaaa 120 ttccaacacc atttattttt atgatagaaa attttattta tttatttgat aaaaaataat 180 gaaagaaaat gacgaggaac ttaataaaaa aatataataa aacaagaaaa aggataagat 240 ttttttaaaa gaatgacaac taagattgga taaaaaaata aaataaaata ctgagggaca 300 aaattgaaag aaaaaacaaa ttaagaaaag gataaaaaag agcaattcca aaaataagga 360 ctaaatctgg aaaaaaagaa atgaaataaa atatcgaggg acaaaattga aagaaaaaca 420 aaccaagaaa attataaaaa aaaacaacaa tcaagagaat gagtaccatg tttgatataa 480 taacaaaaca aaatcaatga aaatggatga aaatgaagaa aaaatccaaa acaaagaaag 540 agcaattaaa agaaagagga tcataattga atttttttca ccattatttt ttttatcaaa 600 ataatataaa ttttactttt tagttgaata aaaaatcaag ttattagtta ggggaaaatc 660 atgttaacac ccattgattt ttatttttgc cattattttt ttttatcaaa ataatgtcat 720 tttacttcct gcaaaataaa aaactcgatt gaccctggta accctgatca ctcgacccaa 780 cccatgactc gggagatgat gggggtcgac tcctaagccg ggttttaaaa gcatgataaa 840 aaaataagca ctaaatatga aatgaaaatc caataaaatt aaacaatgac tgatgaaatt 900 gcgaaaaaaa attaatcaat aagagaacta aaaataacag gaattacaat cataagaata 960 atgaacgaat tcaacaaaaa aaagaattaa aatcaaatga taatggatta aattgaaaaa 1020 taaattccaa ttagaaaaaa aaagattaaa agcaaaataa ataggaataa aaaaatgagg 1080 atcaaatttg acaaaaaaca taaaaaaatc aaatgttaag ggatgaaatt aaaaaataaa 1140 taaatcagat tcgatacaat tagaaaattg agaaccattt tgcaacttta caaggttaac 1200 acatcttttg atatagagga gagagaaaag aggggtggag aaaatttttt gcttgaactc 1260 cgctgcgcca cgaatgatga catgtgttgg cccaccgtgt ggagggcgac atgacacttc 1320 ggatgccgtt gttaaagacc actaacaacc actgggagga gcctcacgcg ccactcaaaa 1380 ggcgcgagcg ccttccactt gctggcgagt gctctgcgcg ccccaactac tttttttttt 1440 gaattacaga aagatcataa cattcatgac cccacatgat aatttaaaaa aaatctaaaa 1500 acaccccttg accttaaaca agaaaaagtt aattccaagg gttattacag agagcaaaac 1560 actcctaatc ccatgtgata attgcaaaaa taaacctagg tttgagaaac caaagatacc 1620 ctttaccctt agaaaatatt tttgacccca agagctattt ggtaattaga ctatgaaaaa 1680 atatgaaaat taagaaatga ccattgaaca taaaaccttt ttttttcctt ctaaaatcat 1740 gggcgtaatt atactaagca acaaaatgta aaaatactat tccactctta tatgtatttt 1800 atattttgat atctttgaag gacaaattaa tcgttttatt atgaattgca ctgtggacaa 1860 tccacaataa aatactgata ccttttagtt atatgttaaa cattgaagaa atgttttttc 1920 atatttttca cgattttttt atttttatat cttttagttt tccaaattta atagtaaaaa 1980 ttaaattggg taaaattatt agtctaaata tgatgtaaac ataaacatta aaatgataaa 2040 atatttaaag agatatttat ataaaaaaat taatttattg ccatccctat cttttatctt 2100 ctttaacctt ccttgctaga aagaataaaa aaaaatagtt aaaaaatatt cttaaaatca 2160 agacaaaaat taattattat ttccatctcc accataaaat atatttctta ttggtttatt 2220 tgatattata atagcggttg tttttttata aatatattaa ttatttttta aaatttattt 2280 ttaacattaa cacatcaaaa taattttaaa aaattaaaaa attaatttta aataaaaaaa 2340 ccctataata gaacgtaaaa aaaacaaaca cgtattttga attaattacc aatttggcac 2400 aaaccgtgag ctgaattctt caactgccca accaaagagt cattaatttc taggagcatg 2460 aggccaccac tccagctgtg tgccaaatga caagggagga 2500 <210> 30 <211> 2817 <212> DNA <213> Vitus vinifera <400> 30 tatattatgt aagtttgaaa aaaaggataa tatttgataa ggtatttaaa aattatttac 60 ttcaaaaatg cattcaacaa taataatttt taaccattta tctttcatgt tcaatttatt 120 ggagaaaaat tattggtaaa tcattcaact tggtcccatt aataaatagg tactaataaa 180 taggtgagag aatggtgaaa aaaccaaaga gagaataaga aaggtagagt gaccagagtt 240 ttttcattta atagtcagag gtattttagg aattttttga agacaatatc tttcatttca 300 atttctactc tttcattggg ttttaagttt aattataaat tatatgagga ccttatctca 360 attattcaac ccaactggga tagaaacaga attctcccta tttagattag ggaaaattga 420 tttaaggttt ttttttttta aatagagtta acaataaaat acaaactaac aaactttcaa 480 gagaaagaaa aaaaaaacac ttataatatt acaaaaaaaa aaaaaaaaaa acacatccta 540 atcacatagc aaaactcaat attttttctc tctcttcttt tgtcctatta acaaccaaac 600 atgattcaat taattgaatt aaactattaa actaataaaa agagccgatc caacttggtt 660 gttagttgtt ccgtattgca tttgctttta cggatgagga aattgaatta aaattgtaat 720 tagcataaca gacgaagaac tgaatatagt cagggagtag aggaaaagtt tagattatat 780 ttatgacatt aaaaatcaac ctaatccaac ttggttgtta gttgttccgt actgcagttg 840 atttttatgc ataagaaata ttggacggtc caatataata taatattagt tgatatatag 900 ctttcagtta ggttggaagg tggacaattg caagtgccaa gtcaaggtta gtggcaaggg 960 cggctccatc attcacaatt tgcctgccat tacctccctt ttgcttggag ttttcccatc 1020 aagcaataca tttattgttg tttatagtgt taaagggatt atgcaaatat catttcatat 1080 aagctgaagg aaatttcatg aaaaaaaaaa tagcatttat tatttatact atgaaggcaa 1140 ccatagaaat taatatcatt tcataaagtt aaacaaattt aaggcaaaaa ggaatttgtt 1200 gcctatcaca ttaaagaaaa tgatagaatt aagggaagaa aatttagtct atgtttagtt 1260 tttgaaaaat ttgaaaaaaa aatggaaaga aagaaaataa aaataaaaaa taaattattt 1320 ttatatgttt tcttaaattt cataaactta tttttcttca ttgtattaag attaaataat 1380 tttaaaatat ataaattttt aattaatttt aattatattt tattttcttt tatatttttc 1440 atgatgaaat caaacatgaa aaaataattt tttttaatat ttttttttct ttccttatta 1500 tttttcaaga accttaaaaa tgtaaaagtt ttaagtttta agttgatttc tttttctttt 1560 ttttagtctt ttttattttt aggaaatata aataatttta aaaatctgta aggatttttt 1620 tttcatttga taatataatc atatttttat aaataatgaa catatacttt gagactttga 1680 attaatgtaa atttaaataa ttatcctcct aaataatatt tataaaaatt gaatgtagtt 1740 ctcaagaaaa gaaagaatta aaataaattg ttcaattttt acttttttta tttacttaaa 1800 atccaactat tttccaaaat tttgagggag tgagtacata gaatgattca tttttcttct 1860 actcataatt cattttggag attcaaatga atgaaaatta gatagtatag tctattttca 1920 aattcacaag ttgaagaaaa aaaagtgatg atgagtagaa agatgtatcg tcaagttcat 1980 catttaatag gcacaccgcc acaaatcctc attcatcttg aagaatgaac ttagagatga 2040 gattttggaa gtgtacagtg tgatacgtat gagtggtgtg tattggtaca ttaagatgga 2100 taaagatgga aataaaatgg agagaaagaa agagagagag gaggaaaaat agaacatgct 2160 gctgaatagt atagggtaac cggccccatg acttagcaat acgcaattac tgaaaagctc 2220 caaccctcct cccaaccatg agccattaat gtcttaagag aatcaggcca tcactacagc 2280 tgtgccatcc tccctagcaa atgcccctac gcgttctcaa tttggaggac actttttggt 2340 caatttcggt gacgttcata ctttgcctag agacaaaata ttgatcaata ttacaagcta 2400 accgatagag actgattaag agctttaact gtatttggat gcccacttag tttttggagc 2460 tataagtagg tgcattggag ttgggaaaaa taaaggccag agtttgggag acttgcaacc 2520 aagtttcaca aatacagggg cttaatcaag ttgtttcatt atccaattat tttattataa 2580 ggcaattcca acctcttaac ctctcgatct ctttccttct ttctgctgct tctatctggg 2640 aattgtttcc ttccctttcc tctctttctt tttcttgtat ttggcttctt ctactatttt 2700 gggctacaat atctcggatc tttctgttcc cgaactgaaa cttttcctca tcaaaagcct 2760 gtacagatag agaagagttt gcacatatat taagaaagga ataattttgt cggaaaa 2817 <210> 31 <211> 2500 <212> DNA <213> Vitus vinifera <400> 31 tatattatgt aagtttgaaa aaaaggataa tatttgataa ggtatttaaa aattatttac 60 ttcaaaaatg cattcaacaa taataatttt taaccattta tctttcatgt tcaatttatt 120 ggagaaaaat tattggtaaa tcattcaact tggtcccatt aataaatagg tactaataaa 180 taggtgagag aatggtgaaa aaaccaaaga gagaataaga aaggtagagt gaccagagtt 240 ttttcattta atagtcagag gtattttagg aattttttga agacaatatc tttcatttca 300 atttctactc tttcattggg ttttaagttt aattataaat tatatgagga ccttatctca 360 attattcaac ccaactggga tagaaacaga attctcccta tttagattag ggaaaattga 420 tttaaggttt ttttttttta aatagagtta acaataaaat acaaactaac aaactttcaa 480 gagaaagaaa aaaaaaacac ttataatatt acaaaaaaaa aaaaaaaaaa acacatccta 540 atcacatagc aaaactcaat attttttctc tctcttcttt tgtcctatta acaaccaaac 600 atgattcaat taattgaatt aaactattaa actaataaaa agagccgatc caacttggtt 660 gttagttgtt ccgtattgca tttgctttta cggatgagga aattgaatta aaattgtaat 720 tagcataaca gacgaagaac tgaatatagt cagggagtag aggaaaagtt tagattatat 780 ttatgacatt aaaaatcaac ctaatccaac ttggttgtta gttgttccgt actgcagttg 840 atttttatgc ataagaaata ttggacggtc caatataata taatattagt tgatatatag 900 ctttcagtta ggttggaagg tggacaattg caagtgccaa gtcaaggtta gtggcaaggg 960 cggctccatc attcacaatt tgcctgccat tacctccctt ttgcttggag ttttcccatc 1020 aagcaataca tttattgttg tttatagtgt taaagggatt atgcaaatat catttcatat 1080 aagctgaagg aaatttcatg aaaaaaaaaa tagcatttat tatttatact atgaaggcaa 1140 ccatagaaat taatatcatt tcataaagtt aaacaaattt aaggcaaaaa ggaatttgtt 1200 gcctatcaca ttaaagaaaa tgatagaatt aagggaagaa aatttagtct atgtttagtt 1260 tttgaaaaat ttgaaaaaaa aatggaaaga aagaaaataa aaataaaaaa taaattattt 1320 ttatatgttt tcttaaattt cataaactta tttttcttca ttgtattaag attaaataat 1380 tttaaaatat ataaattttt aattaatttt aattatattt tattttcttt tatatttttc 1440 atgatgaaat caaacatgaa aaaataattt tttttaatat ttttttttct ttccttatta 1500 tttttcaaga accttaaaaa tgtaaaagtt ttaagtttta agttgatttc tttttctttt 1560 ttttagtctt ttttattttt aggaaatata aataatttta aaaatctgta aggatttttt 1620 tttcatttga taatataatc atatttttat aaataatgaa catatacttt gagactttga 1680 attaatgtaa atttaaataa ttatcctcct aaataatatt tataaaaatt gaatgtagtt 1740 ctcaagaaaa gaaagaatta aaataaattg ttcaattttt acttttttta tttacttaaa 1800 atccaactat tttccaaaat tttgagggag tgagtacata gaatgattca tttttcttct 1860 actcataatt cattttggag attcaaatga atgaaaatta gatagtatag tctattttca 1920 aattcacaag ttgaagaaaa aaaagtgatg atgagtagaa agatgtatcg tcaagttcat 1980 catttaatag gcacaccgcc acaaatcctc attcatcttg aagaatgaac ttagagatga 2040 gattttggaa gtgtacagtg tgatacgtat gagtggtgtg tattggtaca ttaagatgga 2100 taaagatgga aataaaatgg agagaaagaa agagagagag gaggaaaaat agaacatgct 2160 gctgaatagt atagggtaac cggccccatg acttagcaat acgcaattac tgaaaagctc 2220 caaccctcct cccaaccatg agccattaat gtcttaagag aatcaggcca tcactacagc 2280 tgtgccatcc tccctagcaa atgcccctac gcgttctcaa tttggaggac actttttggt 2340 caatttcggt gacgttcata ctttgcctag agacaaaata ttgatcaata ttacaagcta 2400 accgatagag actgattaag agctttaact gtatttggat gcccacttag tttttggagc 2460 tataagtagg tgcattggag ttgggaaaaa taaaggccag 2500 <210> 32 <211> 456 <212> DNA <213> Zea mays <400> 32 aagcttgctt tcacatctcc tccggtcctc ctccatgtgt acaccaaaca aaccaagaag 60 aaaaaaatgc atggttttgt tcccgattta taccttggag caacccttcc attctcttga 120 aattttcttt gtcgcccctt gaacttcccc ccatatgtcc agtccagcta caaattaatg 180 gcgacgagcg atgaaacgca cggaacatat atgcctcagc aggcaaacgt cccagtcgtc 240 gatctctggc acttaattaa ttgtttatat taattccaac tcgttcgtgc aacggtgcaa 300 acaaaacaag ctgacgatgt cgagtcgctg tttccccttc caccttaatt gcttttgcag 360 ccaaacctcc tgctggacaa ccagcagctc cagctactgc agcagcaaca cctgctggac 420 cagatcccgg cgacgacggc ggagagcggc gacaac 456 <210> 33 <211> 2980 <212> DNA <213> Setaria italica <400> 33 gaacgacgga aactgctagc gtaactatag aattctggaa aggataggca cgtcgttttt 60 cagtccactc cgtacagcga cagaaacaga cgtctctcaa aaaatcagta caggtgtaat 120 gacatttaca gccacgaagg atcgttttct ttgctataca tactcaggtt tttctctact 180 tatactaatc tttcgctaat ttatctctga ccagtaaaag gctgtgtttc ctattgtgcg 240 cagaaagatc agaccacacg ccaagagccg tgccgaggta tatatggaaa cagtacacta 300 ctatctgcag cagtgcagca tcgatttctt cgccccttct cctgaaaagt cgagatataa 360 aaagatggag aaaacctcgg tcaaatttgg attagttgca tgcatgcatg catttcatcg 420 cgaggattac ctccgtttta aaatgcttga catgattttc aaaaccacat ttaacaattt 480 gtcttattaa aaatcaatat attatgtaaa aatataaatt atatttaaaa tatatttaat 540 aataaaacaa gtcacaatta aataaactat actccttaca taggtttata ggtttctgaa 600 atctgaacaa gacgattatc atgtgcagag tccaaacgtc aacggtgtaa aacaaacact 660 tgaaacccga agtaaatata ccttcatcca acaacattga caatactact acttcctccg 720 tttcaaattg taggtcattt tagcttttct aatattatta tgcatccaga catacactat 780 atctagtgca tagaacaact atacgcctag aaaagtcaaa acgacctata aaggtataaa 840 ggagtggtag ttgcttatcc aagatgatta gcgaattgca tcttagaagt ggataagatg 900 cttaaatttg caccgtactt tggtacacat ggtagctttc ctcatgggaa ttaacttcaa 960 accgctctta tgacccttca agtacttgtg tttaagcaca caccttgcat tttagatgcc 1020 ataatataca tttttttgtc ctttttaagg cattcatttt ttttagtttt gacttgtttt 1080 gttattgtat tactatcaca agccgatgat gcatcatgca tgtaattacc tgacctattt 1140 cactcaggct atttctagtg gagagtttca tctcacagtt tccaagaatg acatgtcatc 1200 aaaagaggtt tgatttctag tggagagttt cacctcacag tttccaagaa tgacatgcca 1260 tcaaaagagg tttgagttga tgaatgaaac aatacccccc aatgcagagt tttatttcac 1320 acccatacca tttaattgtg aggcctgtga ttggatatgc taagatgaaa tgaaactcta 1380 tagccccaaa tgcaagtttc aacaggtttc gtagtcttga aaacagtgca tacacagttt 1440 catcttgaga gcaagtttaa taacgcagct agcaagcggg ctgcaaggtt tctctcagcc 1500 ttttcctagc ccactcgtac aatggttaac tcttcaccat taatacatga tccacaagtc 1560 attctcacaa agtttcttgg ttcctgtgcc caagtcggct ataagcttat agcccgcttc 1620 tcctctctca cctcttctct ctcctccacg tcagcattca gccagcttac agcctcttat 1680 tatacttgct ctgatgaaac tcttttcctc tctcacttca taattacctt gccatgttat 1740 caatgtgtca tcgtatttaa cgtatatgaa atttctatga aacttgcact ggactagctt 1800 ttttgttttt gcgaaacacc gtcgacatcg agagcagtgc ttagttggct gctgttcaca 1860 caggtcatca aagaaatgaa gcactgcttt aaacatttgc tcttatactt ggctacaatg 1920 gaagaggcac ctaatttgga cgctccatat gctatacgat gaatgtaaaa gtagcagtgg 1980 agaaccgaga acctcttcct tctccatcac tcaataacta cggcggttat ctagtcttaa 2040 aaatttgtca acagtgcatt attcatgaac aggacgtacg tccactgcca aacaatctcg 2100 tagccggaaa ttaaataacc aagaaaaaaa tgaaaaatac accggcaaat taaattatgt 2160 aagcctatct ttacatggaa aatgcatgat tgggcttgct gccttcgtca attttctcct 2220 ttatggctgt acacatatat agcttgaaat aaatatgtat gacatgcaga tatatgaacg 2280 tttattttca cattttccac gaaaaaaagt atataatgac gtccaataaa tccaaaatac 2340 atgacatatt tcgcatattt cgccgtattg tagcacacat attgttgacg aaaataatat 2400 aaaaatcatg cacccgtctc gctctctctc tcctagcaat tacgatacgc cctctcaata 2460 aatgcggatt gcaccacctc aacactcgtc cgcaataact ccttcccctc ttcctccaca 2520 ttctcttcca cctccgttcc cctttcttct cttcctcttc ctcctcctcc tcccttcccc 2580 ttccatggct gtctagctcc tcaggatctc tccgtctctc cacttcctgc tagctaccct 2640 tctgagcttg cacgcggtaa agaatcatct cgagagctgc aacttgcagg cagacataga 2700 gagagatcag agttctattt ttgcaaaggc gcacaacata tatagttctt ctgctagatc 2760 tcactgccag tctcacagag cgaaggttta tttgggctag tttgccccta gatctgttgc 2820 tggctgtgtt gttgcttggc aggcaaggta tctctatata ggaataggat tagcgcctgt 2880 tctttggctg tagagagaga gagttgtcct atgtgtctct gaagaaatgg aaacgaatga 2940 gacggcaaga aattagggtt tgcgaggtgg tcagaggaga 2980 <210> 34 <211> 2500 <212> DNA <213> Setaria italica <400> 34 gaacgacgga aactgctagc gtaactatag aattctggaa aggataggca cgtcgttttt 60 cagtccactc cgtacagcga cagaaacaga cgtctctcaa aaaatcagta caggtgtaat 120 gacatttaca gccacgaagg atcgttttct ttgctataca tactcaggtt tttctctact 180 tatactaatc tttcgctaat ttatctctga ccagtaaaag gctgtgtttc ctattgtgcg 240 cagaaagatc agaccacacg ccaagagccg tgccgaggta tatatggaaa cagtacacta 300 ctatctgcag cagtgcagca tcgatttctt cgccccttct cctgaaaagt cgagatataa 360 aaagatggag aaaacctcgg tcaaatttgg attagttgca tgcatgcatg catttcatcg 420 cgaggattac ctccgtttta aaatgcttga catgattttc aaaaccacat ttaacaattt 480 gtcttattaa aaatcaatat attatgtaaa aatataaatt atatttaaaa tatatttaat 540 aataaaacaa gtcacaatta aataaactat actccttaca taggtttata ggtttctgaa 600 atctgaacaa gacgattatc atgtgcagag tccaaacgtc aacggtgtaa aacaaacact 660 tgaaacccga agtaaatata ccttcatcca acaacattga caatactact acttcctccg 720 tttcaaattg taggtcattt tagcttttct aatattatta tgcatccaga catacactat 780 atctagtgca tagaacaact atacgcctag aaaagtcaaa acgacctata aaggtataaa 840 ggagtggtag ttgcttatcc aagatgatta gcgaattgca tcttagaagt ggataagatg 900 cttaaatttg caccgtactt tggtacacat ggtagctttc ctcatgggaa ttaacttcaa 960 accgctctta tgacccttca agtacttgtg tttaagcaca caccttgcat tttagatgcc 1020 ataatataca tttttttgtc ctttttaagg cattcatttt ttttagtttt gacttgtttt 1080 gttattgtat tactatcaca agccgatgat gcatcatgca tgtaattacc tgacctattt 1140 cactcaggct atttctagtg gagagtttca tctcacagtt tccaagaatg acatgtcatc 1200 aaaagaggtt tgatttctag tggagagttt cacctcacag tttccaagaa tgacatgcca 1260 tcaaaagagg tttgagttga tgaatgaaac aatacccccc aatgcagagt tttatttcac 1320 acccatacca tttaattgtg aggcctgtga ttggatatgc taagatgaaa tgaaactcta 1380 tagccccaaa tgcaagtttc aacaggtttc gtagtcttga aaacagtgca tacacagttt 1440 catcttgaga gcaagtttaa taacgcagct agcaagcggg ctgcaaggtt tctctcagcc 1500 ttttcctagc ccactcgtac aatggttaac tcttcaccat taatacatga tccacaagtc 1560 attctcacaa agtttcttgg ttcctgtgcc caagtcggct ataagcttat agcccgcttc 1620 tcctctctca cctcttctct ctcctccacg tcagcattca gccagcttac agcctcttat 1680 tatacttgct ctgatgaaac tcttttcctc tctcacttca taattacctt gccatgttat 1740 caatgtgtca tcgtatttaa cgtatatgaa atttctatga aacttgcact ggactagctt 1800 ttttgttttt gcgaaacacc gtcgacatcg agagcagtgc ttagttggct gctgttcaca 1860 caggtcatca aagaaatgaa gcactgcttt aaacatttgc tcttatactt ggctacaatg 1920 gaagaggcac ctaatttgga cgctccatat gctatacgat gaatgtaaaa gtagcagtgg 1980 agaaccgaga acctcttcct tctccatcac tcaataacta cggcggttat ctagtcttaa 2040 aaatttgtca acagtgcatt attcatgaac aggacgtacg tccactgcca aacaatctcg 2100 tagccggaaa ttaaataacc aagaaaaaaa tgaaaaatac accggcaaat taaattatgt 2160 aagcctatct ttacatggaa aatgcatgat tgggcttgct gccttcgtca attttctcct 2220 ttatggctgt acacatatat agcttgaaat aaatatgtat gacatgcaga tatatgaacg 2280 tttattttca cattttccac gaaaaaaagt atataatgac gtccaataaa tccaaaatac 2340 atgacatatt tcgcatattt cgccgtattg tagcacacat attgttgacg aaaataatat 2400 aaaaatcatg cacccgtctc gctctctctc tcctagcaat tacgatacgc cctctcaata 2460 aatgcggatt gcaccacctc aacactcgtc cgcaataact 2500 <210> 35 <211> 3000 <212> DNA <213> Vitus vinifera <400> 35 tcagactgtc ttgagccatc atcatcatag tttaacccat ggttgtaaag gatccaatca 60 gagtcacacg ctgcacgcgc gcgcacacat ttagagggat tcctttttgt ctagattgta 120 aaaattataa tttttacgaa ttactataga attaagacaa aatagtaaaa gattcctacc 180 tggcactgta atttttcact ttacacgtgg taaattagtc tgacatatac agtgcacgct 240 catttttaac gcatgcatgt tagtccacct tcaagcagag gctagatgct atgttcgatt 300 caaaaatcaa aacaaaaagg ttgtttcaca ttatgtccga tttgaaaaac caagaaagaa 360 aagaaaatct tgtttcatgt agaaggagat aaaattttag gcaaagatca aaatttggta 420 agaagtaaca taattgttgg tttgtttatc aaatatggca agctttacac ggtaatatgc 480 aaccaatata tgcataatta catttgtatg tagttaatag cataatagca tgcagctaat 540 gtgtacactc ttttgatatg tacatatcac actacaccat ggtgcaagaa tgttagagca 600 tatatagaaa tggggggttt catgaattac aaatatttgg taagcttgtt atctaaaggc 660 aatattaggt tgatattttt ttcaatttgg tgagggaaca aaactgcctt attaacaata 720 tccaaattta tgattgagtg gaataagaaa aattaagggt gccctgaaga cagccattag 780 cccacagatt ttggtacaat aagatacgtt atcaagctaa gagatttgtg caagggaggc 840 atgaatatag taaatgattt attctgcatg cagaccctgc tcgtggctca ggcatttaat 900 ggaatattgc tagggagaag gaggagagat ggggctaaaa tgcttcatca cagctccacc 960 tccacctcct cctcctgata attccctttc ttaattccct cacataccca catgtaataa 1020 tagatctttg tcacggggat tggtgccaaa aacatcatat ttggaacatt aactattaat 1080 ccatgttttc catacatgtt actcacaaaa tcttctgttt atacaataaa ttattggatg 1140 catgtagcaa aatcctttta aggggtgatt cattagggca aatcgtgctt tatggacatg 1200 gaagttcaac tcaattgagg cgtaaacagt gaaaacagtg tcacaaaaca accatttgta 1260 aaagcttaaa aggtactgta tatgagaagc gattaatgat atacaaacaa ggagagaaac 1320 aataaaagaa gtttgaaaag gagttggaga aaaggtgatg ggagaatgag aaaaaaaaaa 1380 aaaggaataa aagaaaaaga aagagttgaa aggagtgctg cagttaagat gggtgtaatt 1440 ggcttcacca tacacgctct ctcaaagaca tctaaacatc aaacgcttat atgcttctgt 1500 atttctatat atatttctct ctccttagca attacacctc catccaccat aaatgctatt 1560 gcaccctctc aatacgctct gcaataactc ctcgctgcca catcttctcc acctgttacc 1620 ctttcttctt ccctttctca ccttctccaa agctctctgc ctttcgagtc tatctcctct 1680 ttttcgccgt ccctttattc tccctctctc tctctgcctc ttcctctctc agtgttcttc 1740 tcaaaacctg ttactctcat ctaaagctgc cagaaaacca ctctttcagc gcccaaaaag 1800 tggttttccg gtgacccaat agtctctaaa acagatctgc cactttgctc actggacttg 1860 accttcatct tcaaaagggt cgttttgctt ttctcccata gcttccgcac ctgtaagtta 1920 ttttgacccc ataggtcttc tccatttttc accagtcttt atttattttt attttttttt 1980 gagtgggggg tctaaatggg gttgcaacac catcattctc atccttaact gtcttgagac 2040 acaagatttt ttcacctttc tttgcatatt ttattaactt ctgagttttt gctctatttt 2100 ccgttatctt ttttgcagta gagaaagagc aaaggttgag gttgaggttg gacgcaccga 2160 gatcttcgtg ggcgttatgg tttgaaattg gtgtttcaaa gcccattcca tactgtccac 2220 tctatcttcc atttttctcc agtaagagct ttgtgaccta cgaacagaaa tacagggtca 2280 agatcttctt tcaaagccca aagccacagt tgtagctcct ccactgctgc atgtttaggg 2340 tttgtggggg cagtgacact tcccggccgg aataatgctt ccggctaatg actggccact 2400 gccaattgaa aggaacctaa aggttgctgg atttgggttg tcatcaggac taattctaag 2460 cgtggtaagc cttcgtggca gtccaagttc tattgatgtc catttcatgg actgtgagtc 2520 tgtgaagact gttagggttg tctgggtttg ggtttttctt ttatcatttt cttagtcatt 2580 tcattgtttc ctcatctctc ttttttcttt ttctttttct ttttctaaat atatatacat 2640 attcaagatc aattagatta gtagagggtt tttgctaggc attaaggtat ttcaagaaaa 2700 atcattctcg atacaaacgc taaaaagctg ttttgcagat gagagagaga gtcttctcat 2760 aaacaggttt tcaaaagtgg gatagggatc cataacctaa gaaaattaat tcactgctta 2820 atactatgta cagatttcca cacatgatta attcttttct tcttaattca aattcttgag 2880 ggtttttttt tttttttttc attctttaca tgggcttctt ccttttgaag tggttgactc 2940 atttcattct cttggcagat tgaagaaccc tagctttgga atcataaagg atctgtgaat 3000 <210> 36 <211> 3134 <212> DNA <213> Populus trichocarpa <400> 36 aattaatctt ttttttgttt gatttagttc gttaatatta attttttttc tatttagtta 60 tcacactctc aggatacgga tcccaggttt gacgggttaa cccagttgat tcagaatgtt 120 tttcttttta atttggtttg cataattttt gttagagttt ttggattcat acatgtgttt 180 ggaaaacacc cctcaagcct atttttagtg gttttattac aaagaaaaat ggatgtttgg 240 ccaaacaaag cctaaaattt ttttaaatat aaaaaaacat cttttttctt gcatatggct 300 aaaaactcct aaagttattt aagcatttct tttataaaaa aaatttaaaa tacttttata 360 tgttttttct aaaaaaaaca tattgcataa attttacaat aagtttgtaa aatttctaag 420 gattttggcc tacatttcag aaatacaaaa aaaaattatt ttgtttcttt taatatgtga 480 gattatgaac ttatatgtaa gacttattcc tgctattaaa taaaaaacaa ttttttttat 540 attggcacta gaacgattaa gtttttaacc tgataagatg aagaccttct tacagagaaa 600 gacttttctt aaaccctaga atgaccaacg gttagaaaac acaacattac cttagtttac 660 gttagacaaa taaacaatac agtttacctc aggtacgacg tattaggggt gttaatacct 720 ttcttttata caatcagttc tcttccccag actcagagac cagttagggt ttctagtgat 780 caaaatatta agtgacaact ttcatttctt ttttttttct actgataaaa gacaagtacc 840 cttcttcttc ttattttttt tttaatgttg tcaatttatt tataaataag agacaaaaaa 900 aagaaaaaaa atattgtcca tttattaaac aaattgttat ctaaatttgt gtgtgtataa 960 tcattttcat ttattagata ggcatttctt acaatgattt taagttcatg tagactttaa 1020 tttattgtgt gttatatgtg atatatattt cacaactcat gaaaattata atgattttgt 1080 aagataacat ctcctatttc tgaccccaga aaactcctag atatattctc attaattacg 1140 tttttattta aacaataata ataataataa taataataat aataaataaa ctatatatat 1200 agtttattta ttattattat tattattatt atatatataa tttgattgtg tgtataattt 1260 agccatattt tttcttcgtt aatcatgttg ttcctaacta ggcatcaaga ccacaaaatc 1320 atgaggaaat tatgatttca tacacgaata ttgatcatct acgtacataa atttttgcac 1380 tggtgaagca acaacaatgg tggaaatttg gaatccaaga agccgtaatt cacaccacca 1440 tacttctaga ggaaatttaa acatgaaata atgatcgctc cttttcatct ctttctcact 1500 ctctcgcatt tacacctctc tcccaccata aatgctagca aaccctctct atacgctctg 1560 caattactcc tctgctgcca catcctcacc acctgttccc tttcttcctt tctcagcttc 1620 ttcaaaacct tataatttgt ctcctccttt tcctcttccg tttctctctc tgtgtgtgcc 1680 cttttttctc ccatcatagt ttaatcatac agtcatctta aaaccctttt cagaacatag 1740 ggtctctctt caaaaaccag ccagtaaacc acttgtctag ccctaaaaac tggttttgta 1800 gagacccaaa acctcttcca tcacaaatct aatccttgtt tccatggaac ttcaaaggct 1860 tctatatttt ggtaagttgc acaccttgtc tataaaacct ttcaattcat ttctcttcac 1920 cataagattc tctcattttc agttttatat catgtcctaa tctttttcct aaacataaaa 1980 atggtgtttt ttattatttt ttgtgcagga attgaaaatg tcgagtggga tggattgacc 2040 tagatcttag tgggcgttat agtcttggta atggtgtgtt gaaatttatt tgtcaaagct 2100 tcagagaggg gtccatttag tgtcaactct tcatgctcat ttgttttttt atataatttt 2160 aatcttttgg aacgtaaaac tagaaaggac ttgagacttt tctttgaagt tatgacccaa 2220 acaccctaca gtcaatcctc ctctcttact acttttcttt gatgttatga ccgacccaaa 2280 caccctacag tcaatcctcc tctcttacta cttgtaataa tctttttttt tggcttttgc 2340 tcaaacatct tgagagaatc tatttcgcaa accaacccca catacagcca acgtaagcag 2400 caaaatcaat catgtcatat cctcaagaga tagaaagaga gaaagataag gatgctgctt 2460 agggtttgtt ggtgagagta agatatgcct tctacaatgg agtttaaagg agaaaaaggt 2520 tgtcagattt caatcatcct ctaaggttag tcaatcaggt aaggttcctc tcactttggt 2580 tgaggttctt aaaaagtgtt tttattcact aaatatgctt aaacaaagta attaaaacgt 2640 ttccaagcaa catccttctt ggaatgaacg cataacgctt tttttttttt gagtaaaaag 2700 agagagagag agagagagaa gagagaggaa gataaaaggt gttatcttgc ttatctggtg 2760 attagccatc tcttgtaact acagctacga cagtgtggaa tacacttcac cttgccctac 2820 aaatttcaaa caacatttat tatgttggga atttaacttg gttttgcttt ttttcctcct 2880 tgtactcttt tttataattc atcaatgttt cctatcaata aatctttcca tatagatgtt 2940 aatttcttat ctacaaacat ctcatatata gcctttaata cttttcttta cttgtacaca 3000 tgatttactc acccctttag catgtttaca tggcattcct taggattatt cttctttttt 3060 tatttgctta tttatgattt tttatttgca gattggaagg acagcttagc ttaaaattta 3120 agggatttat acaa 3134 <210> 37 <211> 3091 <212> DNA <213> Populus trichocarpa <400> 37 aattaatctt ttttttgttt gatttagttc gttaatatta attttttttc tatttagtta 60 tcacactctc aggatacgga tcccaggttt gacgggttaa cccagttgat tcagaatgtt 120 tttcttttta atttggtttg cataattttt gttagagttt ttggattcat acatgtgttt 180 ggaaaacacc cctcaagcct atttttagtg gttttattac aaagaaaaat ggatgtttgg 240 ccaaacaaag cctaaaattt ttttaaatat aaaaaaacat cttttttctt gcatatggct 300 aaaaactcct aaagttattt aagcatttct tttataaaaa aaatttaaaa tacttttata 360 tgttttttct aaaaaaaaca tattgcataa attttacaat aagtttgtaa aatttctaag 420 gattttggcc tacatttcag aaatacaaaa aaaaattatt ttgtttcttt taatatgtga 480 gattatgaac ttatatgtaa gacttattcc tgctattaaa taaaaaacaa ttttttttat 540 attggcacta gaacgattaa gtttttaacc tgataagatg aagaccttct tacagagaaa 600 gacttttctt aaaccctaga atgaccaacg gttagaaaac acaacattac cttagtttac 660 gttagacaaa taaacaatac agtttacctc aggtacgacg tattaggggt gttaatacct 720 ttcttttata caatcagttc tcttccccag actcagagac cagttagggt ttctagtgat 780 caaaatatta agtgacaact ttcatttctt ttttttttct actgataaaa gacaagtacc 840 cttcttcttc ttattttttt tttaatgttg tcaatttatt tataaataag agacaaaaaa 900 aagaaaaaaa atattgtcca tttattaaac aaattgttat ctaaatttgt gtgtgtataa 960 tcattttcat ttattagata ggcatttctt acaatgattt taagttcatg tagactttaa 1020 tttattgtgt gttatatgtg atatatattt cacaactcat gaaaattata atgattttgt 1080 aagataacat ctcctatttc tgaccccaga aaactcctag atatattctc attaattacg 1140 tttttattta aacaataata ataataataa taataataat aataaataaa ctatatatat 1200 agtttattta ttattattat tattattatt atatatataa tttgattgtg tgtataattt 1260 agccatattt tttcttcgtt aatcatgttg ttcctaacta ggcatcaaga ccacaaaatc 1320 atgaggaaat tatgatttca tacacgaata ttgatcatct acgtacataa atttttgcac 1380 tggtgaagca acaacaatgg tggaaatttg gaatccaaga agccgtaatt cacaccacca 1440 tacttctaga ggaaatttaa acatgaaata atgatcgctc cttttcatct ctttctcact 1500 ctctcgcatt tacacctctc tcccaccata aatgctagca aaccctctct atacgctctg 1560 caattactcc tctgctgcca catcctcacc acctgttccc tttcttcctt tctcagcttc 1620 ttcaaaacct tataatttgt ctcctccttt tcctcttccg tttctctctc tgtgtgtgcc 1680 cttttttctc ccatcatagt ttaatcatac agtcatctta aaaccctttt cagaacatag 1740 ggtctctctt caaaaaccag ccagtaaacc acttgtctag ccctaaaaac tggttttgta 1800 gagacccaaa acctcttcca tcacaaatct aatccttgtt tccatggaac ttcaaaggct 1860 tctatatttt ggtaagttgc acaccttgtc tataaaacct ttcaattcat ttctcttcac 1920 cataagattc tctcattttc agttttatat catgtcctaa tctttttcct aaacataaaa 1980 atggtgtttt ttattatttt ttgtgcagga attgaaaatg tcgagtggga tggattgacc 2040 tagatcttag tgggcgttat agtcttggta atggtgtgtt gaaatttatt tgtcaaagct 2100 tcagagaggg gtccatttag tgtcaactct tcatgctcat ttgttttttt atataatttt 2160 aatcttttgg aacgtaaaac tagaaaggac ttgagacttt tctttgaagt tatgacccaa 2220 acaccctaca gtcaatcctc ctctcttact acttttcttt gatgttatga ccgacccaaa 2280 caccctacag tcaatcctcc tctcttacta cttgtaataa tctttttttt tggcttttgc 2340 tcaaacatct tgagagaatc tatttcgcaa accaacccca catacagcca acgtaagcag 2400 caaaatcaat catgtcatat cctcaagaga tagaaagaga gaaagataag gatgctgctt 2460 agggtttgtt ggtgagagta agatatgcct tctacaatgg agtttaaagg agaaaaaggt 2520 tgtcagattt caatcatcct ctaaggttag tcaatcaggt aaggttcctc tcactttggt 2580 tgaggttctt aaaaagtgtt tttattcact aaatatgctt aaacaaagta attaaaacgt 2640 ttccaagcaa catccttctt ggaatgaacg cataacgctt tttttttttt gagtaaaaag 2700 agagagagag agagagagaa gagagaggaa gataaaaggt gttatcttgc ttatctggtg 2760 attagccatc tcttgtaact acagctacga cagtgtggaa tacacttcac cttgccctac 2820 aaatttcaaa caacatttat tatgttggga atttaacttg gttttgcttt ttttcctcct 2880 tgtactcttt tttataattc atcaatgttt cctatcaata aatctttcca tatagatgtt 2940 aatttcttat ctacaaacat ctcatatata gcctttaata cttttcttta cttgtacaca 3000 tgatttactc acccctttag catgtttaca tggcattcct taggattatt cttctttttt 3060 tatttgctta tttatgattt tttatttgca g 3091 <210> 38 <211> 3940 <212> DNA <213> Medicago truncatula <400> 38 tgattactct agagagtaag ttttttccaa cacatccaac taatatctct atgaccactc 60 ttcatacaac aaggaacact cacacacagt aagaaggcat tgcgaaactc ggtctaactt 120 gttctcttct aggccactat ttcaaaacaa aaaagtttct cacaaacaat tgttttttaa 180 gaaattaata tgggatatat taacaacaac gataagctct ttagtataag gtgtactaga 240 gaaaccatct aaaaaaataa actgtcatat aaagtgcgtt tgatttgcta aaaaaatgaa 300 ggacaggaca gaacaactct agttgtccag tgtttgattt gtaaaactgt ttcaggtaca 360 ggacaaactg gaggcaaggg acaggacaaa aactcatatt tttgtccctc accaaaccac 420 aacacaactt tttgtcccac gtacaaattg tctaaaatac caaaatagta ttttgtccct 480 aaaaaatcgt aaaacaaaaa aattactcaa tgccataaaa aatttgtcat gtgttgttct 540 gccttgtgtt gtactgttct atcttgtact gttctgtcca gtacttgttg ttttgcaaac 600 caaacacacc cataaagtat atacaagaac aaaggctagt caatacccat acaaagcaaa 660 tgatccgact accatctctg agaaccgtac acaaacgtgg cgttattagc ttttaaccac 720 caaaacgaat taaatttaat ttatctaaga gttataagat aggagtttca atgttgttga 780 atagcctatt attgcgatca ttctcacaaa taataagtat aatctgtatt tttttttgaa 840 ggaagtataa tctgtactta atggatccaa aagttaacca taagtaacta ttaatttcgg 900 ttggacattt tttgtaacat atttaccctt aaaacaccgt tcttttaaaa tttaaatgta 960 aatatacatc ataattaaat aggtccttaa aaaaaatcat aactaaatag gactattaaa 1020 caaattacaa atttaacaac aatacagttc aatccaaatt caaattttaa aaaatataaa 1080 acatgattat tgaaaaataa atttagatta agatatcttg tacaattatc ttatcttttt 1140 tatctgaata attgtactcg tcttatcttt ttttaaatca tagcttgtgt acccttaaaa 1200 aaaatcatac cttgtgtaaa aattcataat catatttaca tcgttccttt tcattttata 1260 actaatttct tttcttttga ttgcagggat tgattcttca ataacttcga caagaaagtc 1320 gttaggacga acactttaaa ttgaaagaca actttcatga cgacatgttg gtacttgtga 1380 aagtggagga atataactat tttttaagct aatttcatcc gctcgaacaa cccaatttct 1440 ttaattcgga gattcgtcca agatattgaa gactgtaact tggagcatct tcatattggt 1500 gcattgttga aggacatcat ctatataagt tggaagcggc ctcaagaagg ttggaccaag 1560 ctcaacatga tggtgcttgc aaagatacga gggttattgc tggatgtggc gatctttttc 1620 gtgattcaga cggtagatgg atcaaaggct acactaagaa gattggagct tgtgatgcct 1680 tatatgttga gattttgatc gacatgattt ctgataattt caagttcaat gagaatattc 1740 ctgttttagt ttaccggatc tgaaagttgc cgaagatgag ttgaaatgtt caactcaacc 1800 atacttgacg tgaaggaaat aggagtgctg attggctcgt taacttaagc atttctacgg 1860 atcactttaa tttactaatg ttggagtctc cttctagcga ggtccaaaga atccttttag 1920 atgatttatc caggacttgc atgcctagga atcttcgatt agctttttag ttctattttt 1980 ttttctttgc cctcttgtaa aaaaataaaa ataaagatct ttgaaagtac agaatgtatt 2040 tatatatgta atttttttcc tcaaatacca atatatacta ctagtataag tagagtatat 2100 ttacattatc gtagagtacg tgaatgaata taagtaaatt cagtagggaa gagtaaaaat 2160 taacgagttt ggtagccgtc atcttttgca ccatctactc tcaaatccaa ttttttttac 2220 tacacaacac aataatctct ctctctctct ctctctctct ctctctctct ctctctctca 2280 catagtctct ctcatgtaca ctactagcaa ttactacacc tcttacgccc accattaaat 2340 gcaattgcac catctcaata cactcagcaa taactcatca ctgccacaat tttcctctcc 2400 atccgttcac tcttatttcc tttctcttcc ttcaattcct ttgtcctctc accttcttca 2460 tcgtcttcat cttcttttct tcttctttct ctctttaaac aatctactac cttttttaag 2520 ctagaccgtc caaaaacact catcaagctt tcgttgctta atttattacc atatacccaa 2580 ctttccataa cttcctcaaa aaaaccttct cttctttttt catattcatc tttgaaaaaa 2640 gtgtcttttc tttttacttc tttttgtgca ccatagtaga tctacatttt ttcttcctct 2700 tctagcttct atctctcttt agcaactacc cttcctcttg gtaagtggaa aaaaaaagtc 2760 tttgttatca ttaattcatt tcacatctca ttcacaccaa tatcattgtt tttgtaaaac 2820 taaatctgtt cacaagtttt tttcttttaa aaatacttat gtctccgtcc atatagttag 2880 ggttgctaga atgtcatata tttatttttt gttttttttt ccatcttttt tctttctttc 2940 tgattatgct tgttcctatg ctgatttcaa atataacaaa agaatttaac atgcatttta 3000 attatgctta aaaattctta gaagcataaa gaaaaaaaat ctggactttg atcaccccaa 3060 tattttttag tttttggtta taaagtgagg tcacgttttt atatttgagt attttttttt 3120 ctctcatgtt attgatgttt ttgtttcctt tttgaagtgg cacattaatc tcattgaaac 3180 accgctaaag ctgacagaag aatctaacaa agaattcatc atctgaaagc catttaagga 3240 tctaagaaaa gagagagaaa aaattaaaag aaggattttc tagttgaagc ttttttgatt 3300 gaaatatgca agctggttta atgattccag ctagtaacat gcattcaatg atcggaagaa 3360 acaacaataa cgttggtgtc tttggtttat cttcatctct ctctttatcc caggtgaatc 3420 ttcttgtttt tttttttcct tcacatttct atcaaaattt ttagtatatt aaattaataa 3480 taataataac aattttgttc taatgttaat ttaatttgat aaagggtcat ctattttgac 3540 tttatctttg caaagataat tattttctct taagtcaaca tttggtcttt attgaaagaa 3600 caacctaaac aaacaaaaaa acctttcatt gtattttacc agtccaagta tccttctcgt 3660 tattcggaaa ccgcgttatt ccttttcact ttttcactat ttaaattcat tttcctcaaa 3720 aacatcaact tagtcaaatt cacatgtttt tctttttcat caagtttctc caatttaatt 3780 agaaaaaata aataaacaaa aattgttttt gttgattttg ttttttgttg ttgttgaatg 3840 tactatgtac tatgtatgta ctatactata tattatgtgt taaattgttt ttgttagatt 3900 gaaaacaata agcatttgga atcttaaaga tctgtagaaa 3940 <210> 39 <211> 3897 <212> DNA <213> Medicago truncatula <400> 39 tgattactct agagagtaag ttttttccaa cacatccaac taatatctct atgaccactc 60 ttcatacaac aaggaacact cacacacagt aagaaggcat tgcgaaactc ggtctaactt 120 gttctcttct aggccactat ttcaaaacaa aaaagtttct cacaaacaat tgttttttaa 180 gaaattaata tgggatatat taacaacaac gataagctct ttagtataag gtgtactaga 240 gaaaccatct aaaaaaataa actgtcatat aaagtgcgtt tgatttgcta aaaaaatgaa 300 ggacaggaca gaacaactct agttgtccag tgtttgattt gtaaaactgt ttcaggtaca 360 ggacaaactg gaggcaaggg acaggacaaa aactcatatt tttgtccctc accaaaccac 420 aacacaactt tttgtcccac gtacaaattg tctaaaatac caaaatagta ttttgtccct 480 aaaaaatcgt aaaacaaaaa aattactcaa tgccataaaa aatttgtcat gtgttgttct 540 gccttgtgtt gtactgttct atcttgtact gttctgtcca gtacttgttg ttttgcaaac 600 caaacacacc cataaagtat atacaagaac aaaggctagt caatacccat acaaagcaaa 660 tgatccgact accatctctg agaaccgtac acaaacgtgg cgttattagc ttttaaccac 720 caaaacgaat taaatttaat ttatctaaga gttataagat aggagtttca atgttgttga 780 atagcctatt attgcgatca ttctcacaaa taataagtat aatctgtatt tttttttgaa 840 ggaagtataa tctgtactta atggatccaa aagttaacca taagtaacta ttaatttcgg 900 ttggacattt tttgtaacat atttaccctt aaaacaccgt tcttttaaaa tttaaatgta 960 aatatacatc ataattaaat aggtccttaa aaaaaatcat aactaaatag gactattaaa 1020 caaattacaa atttaacaac aatacagttc aatccaaatt caaattttaa aaaatataaa 1080 acatgattat tgaaaaataa atttagatta agatatcttg tacaattatc ttatcttttt 1140 tatctgaata attgtactcg tcttatcttt ttttaaatca tagcttgtgt acccttaaaa 1200 aaaatcatac cttgtgtaaa aattcataat catatttaca tcgttccttt tcattttata 1260 actaatttct tttcttttga ttgcagggat tgattcttca ataacttcga caagaaagtc 1320 gttaggacga acactttaaa ttgaaagaca actttcatga cgacatgttg gtacttgtga 1380 aagtggagga atataactat tttttaagct aatttcatcc gctcgaacaa cccaatttct 1440 ttaattcgga gattcgtcca agatattgaa gactgtaact tggagcatct tcatattggt 1500 gcattgttga aggacatcat ctatataagt tggaagcggc ctcaagaagg ttggaccaag 1560 ctcaacatga tggtgcttgc aaagatacga gggttattgc tggatgtggc gatctttttc 1620 gtgattcaga cggtagatgg atcaaaggct acactaagaa gattggagct tgtgatgcct 1680 tatatgttga gattttgatc gacatgattt ctgataattt caagttcaat gagaatattc 1740 ctgttttagt ttaccggatc tgaaagttgc cgaagatgag ttgaaatgtt caactcaacc 1800 atacttgacg tgaaggaaat aggagtgctg attggctcgt taacttaagc atttctacgg 1860 atcactttaa tttactaatg ttggagtctc cttctagcga ggtccaaaga atccttttag 1920 atgatttatc caggacttgc atgcctagga atcttcgatt agctttttag ttctattttt 1980 ttttctttgc cctcttgtaa aaaaataaaa ataaagatct ttgaaagtac agaatgtatt 2040 tatatatgta atttttttcc tcaaatacca atatatacta ctagtataag tagagtatat 2100 ttacattatc gtagagtacg tgaatgaata taagtaaatt cagtagggaa gagtaaaaat 2160 taacgagttt ggtagccgtc atcttttgca ccatctactc tcaaatccaa ttttttttac 2220 tacacaacac aataatctct ctctctctct ctctctctct ctctctctct ctctctctca 2280 catagtctct ctcatgtaca ctactagcaa ttactacacc tcttacgccc accattaaat 2340 gcaattgcac catctcaata cactcagcaa taactcatca ctgccacaat tttcctctcc 2400 atccgttcac tcttatttcc tttctcttcc ttcaattcct ttgtcctctc accttcttca 2460 tcgtcttcat cttcttttct tcttctttct ctctttaaac aatctactac cttttttaag 2520 ctagaccgtc caaaaacact catcaagctt tcgttgctta atttattacc atatacccaa 2580 ctttccataa cttcctcaaa aaaaccttct cttctttttt catattcatc tttgaaaaaa 2640 gtgtcttttc tttttacttc tttttgtgca ccatagtaga tctacatttt ttcttcctct 2700 tctagcttct atctctcttt agcaactacc cttcctcttg gtaagtggaa aaaaaaagtc 2760 tttgttatca ttaattcatt tcacatctca ttcacaccaa tatcattgtt tttgtaaaac 2820 taaatctgtt cacaagtttt tttcttttaa aaatacttat gtctccgtcc atatagttag 2880 ggttgctaga atgtcatata tttatttttt gttttttttt ccatcttttt tctttctttc 2940 tgattatgct tgttcctatg ctgatttcaa atataacaaa agaatttaac atgcatttta 3000 attatgctta aaaattctta gaagcataaa gaaaaaaaat ctggactttg atcaccccaa 3060 tattttttag tttttggtta taaagtgagg tcacgttttt atatttgagt attttttttt 3120 ctctcatgtt attgatgttt ttgtttcctt tttgaagtgg cacattaatc tcattgaaac 3180 accgctaaag ctgacagaag aatctaacaa agaattcatc atctgaaagc catttaagga 3240 tctaagaaaa gagagagaaa aaattaaaag aaggattttc tagttgaagc ttttttgatt 3300 gaaatatgca agctggttta atgattccag ctagtaacat gcattcaatg atcggaagaa 3360 acaacaataa cgttggtgtc tttggtttat cttcatctct ctctttatcc caggtgaatc 3420 ttcttgtttt tttttttcct tcacatttct atcaaaattt ttagtatatt aaattaataa 3480 taataataac aattttgttc taatgttaat ttaatttgat aaagggtcat ctattttgac 3540 tttatctttg caaagataat tattttctct taagtcaaca tttggtcttt attgaaagaa 3600 caacctaaac aaacaaaaaa acctttcatt gtattttacc agtccaagta tccttctcgt 3660 tattcggaaa ccgcgttatt ccttttcact ttttcactat ttaaattcat tttcctcaaa 3720 aacatcaact tagtcaaatt cacatgtttt tctttttcat caagtttctc caatttaatt 3780 agaaaaaata aataaacaaa aattgttttt gttgattttg ttttttgttg ttgttgaatg 3840 tactatgtac tatgtatgta ctatactata tattatgtgt taaattgttt ttgttag 3897 <210> 40 <211> 3000 <212> DNA <213> Arabidopsis thaliana <400> 40 acgtacgtat aactccatct atatatctgt acctacatat atacagtaaa aatcagaaat 60 aagttgggtt attgtttttt tatatatagt tattttaaag aatctaggta aagtataaac 120 ttactgaaaa gaaaaacttg agtgttaaag ctatagctat aaggacttta aaaaaaaaaa 180 caaagcttaa gaactaggta attaaatacg cagtatattt ctctatatat tcgcgtgata 240 aattaagatg aatgcatggc ttttacaaat tatcaccttt ttaatgggtg atccgtattt 300 taaatagctt aattgcacgt tatagttata aatgcttggg ccacaaagta attaatattt 360 ctaggcatgt aaaatgaagt cttactgtga aaaaagtgat atttcattga cttccttaaa 420 ttgaaaaaac aattgtttcg taaacaaaac tcaaaattaa ggtgatgatt tttagatttt 480 cttcttttaa atgaaataaa ataaatacat cactcaatca acttctgtct gactaacaat 540 aatacaacct aaacttctat aatatttaag aaaatgccat gtggaagaag aatcatgaaa 600 ttttccttct ttacaattaa atttataaca aagatatgaa ataataataa aataaaatag 660 ttaaaaatat aatagtcata ttttttgaaa gtaaaaatta aatttatatc tcataaagat 720 aaatattaac tataacaagt cattgaattt tagttaaaaa actatataat ttttttccat 780 attataaata atctaagagt attaaaattt cagaaaatta cttttaaaat tgtatccttg 840 acaaatgggg actattgtct aactttttct gtaaatggag aactgtatta aagcaaattt 900 caatatagtt aaatcttttt ttaatatagt taattttgtt aagtggtgat ttattgcata 960 tgcataagtt atgaataatg ctaatgattt tttaaaaata actacatatc taatagataa 1020 atgtaagtct cataggctca tagaccgtta gccgtcattc ctcaccacat tataatattt 1080 taaataaata aaaaataaaa ttattttagg ttgacattgt atataagatg aaaaacaaca 1140 cataattttg taacataaat aaaataaatt gtaacatatc tctctctctc tatatatata 1200 taagcgtgta ggataattaa gtgtccctct cgcaccattt aatttcattg caccctctca 1260 attctctctg tcattactct ctcatctctc ttcctccaag atttgcacct acctttcaaa 1320 ccttttgctc gccaacaaaa ccgcgcttca caaaaccatc agcggttttc cgagaaacaa 1380 atctcagacc agacaccgct ctactcttct ctctctctcc ctctagggtt tcgtcgcttc 1440 ttcctatatc ccagtgtatg tctcgaaaac ctcatcattt ctgctgtttt ctttatttta 1500 tccattaatt catgtacaca aatttatttg tttaaagtat cattggatcc attacacttc 1560 agttttctta gcatctttat aaaactctca ctttcatgat gttttttttt tataatttaa 1620 tatatcctgt atgttgaatt ttcagttagt catctatatt ttgtcttaaa ttttgccttc 1680 tttagcatta attaattgat ttcttgcctc taatatcttt cattcctctc ctaatttgct 1740 ttctgacttt ctgatttatt gtattcttct ttaaataatc ttatcaattt ctgcatagtc 1800 agagatgaaa ccttcaagaa aacaataaag aggaaagatt aaaggttagt attcccttgc 1860 ctaccaaaaa aaacagaaag gtttgtattc tcttcgtata tccttatttt cattatacgt 1920 ttggttttat atataaacac gtgtatatac tttatagtag tagtatacat ttacatatta 1980 atgtgtgtaa taaaaacgtt taaatacgat gatgatgata agaaatagct ggaaaaaatt 2040 aatacatcac ccaggcctta tgtcacgttg gttgtaagtt gtataataac tatatgttgg 2100 tccgtcgcac aggtgcacag atataatact atttttttca tttcaatttt tttttctttc 2160 ttaatcgcca aatagtagtt tacacttttt cctgaagagt ttttattttt tgaaaatcta 2220 ctaatgatta tatttaatta aattttgttt tgttctcacg aaaatattgt ttttttaata 2280 aacaggatag agttgggaat ttatttgtac ctactttttc ccattttatt tgctttgaac 2340 aaatgagtcc aacatttctc ttttcgtctt ctctttccta tcccatctaa atcaaaactg 2400 atcaaaatac ctcgaaattt taaattaaat caccatccat caaagctatt atagtgtaga 2460 aattttaaag ttttttcttt ggacaacaat tgatttccat ctacactaca tatgatatga 2520 ctcttgaatc aatcatagtt tttatttcag tctgctgatt agtttttttc ttttctttta 2580 aaagggtaaa aaatcggtag tttcgaataa ttttgagtat gtgaaaattc attatttttg 2640 ggttggttat tcaataacaa attaatttgt gattgtgatg tcttttttat gcaaggctct 2700 tttttcttgc tttcaattag tgggtccttt gtttcctaca atcatctcca attcttctgt 2760 ttgcttatat cattttgtat gatcttcaag aagaaagata aacgactccg aagaaagatt 2820 taagttattg ttgttcatac aaaaagaagg ttgtaaagtt gttgacgtgg acaaaagtat 2880 tgccatattt ttccagcctc gaagaaattt cccttcttct tcaaacccta aattttgaca 2940 aaactctcta cttttttttg cttctagggt ttgctttcag tttccggagt ataagaaaag 3000 <210> 41 <211> 3641 <212> DNA <213> Glycine max <400> 41 tatatcattt gtttttaatg aagttatttt tggtttagtt tcttttaatg aagttactat 60 aaatttaatt atagttgttc aaactaatat ttagtccgaa caaaatacaa attggctacc 120 gctatgcgct atctaacaaa attttccgag tcataacatg cagatgcatg cggttcaaaa 180 ttaatacttg cactcatcat aaaatttgtt catatatagg taagactttc cacgtcaaat 240 ataaattaaa gtgcatagaa caaaaataat taaattgcaa atgtatttta tggtaaattg 300 caattcttta taataatgta ctatacaata tttatttaga aaacaaaatc ttgcgttacg 360 aattaatttt tttaatgtag ttacgaattg aattacacgg ttacaagtac catgcatttt 420 tatatgctaa gagaaattca tatttttaat caatctttta cgaagtgact agcttacacg 480 gttacactac attgtaatca aattatatta tatgtagtat cttttttctt ttataaaata 540 tattactagt aagtatcttg tgcggatact tttaaacaca aagaaaatat aaaaaataca 600 aaaatatata attttaaaaa tataaaaata taatcaacag aatgattttt cttaataatt 660 tgaaaatatt ttatcatgca taatgttaat aaaagaaact caagtattaa ttaaagaaat 720 taaaaaacta agaaccttct ttaatatgat taattgagga aatatatcga tttaacacat 780 ttcaagaaat aaaaaataca tgcttatgta aaatattaaa aatatatata aatgttatat 840 ctccgttaaa aacacgttta aatttagagt tcaattaaaa tatattgaaa atgttttttt 900 ttgtagtatt atctaatact gtataaatta taccatgtaa gataatattc ttgattcttg 960 taagaattac tttaaaaata tatctaaacg gtttgtaatc atgcaatatt ataaattttc 1020 ttatattccg taaaaaaata atattttatt ataataatat ataaaatcaa actcttatat 1080 tcataatata ggaaatagta gtcttcaaaa aaacaggaac gaataaatca tctcaatctt 1140 atcatatcaa ttattaatta cctaatttct ttaattaatt aaattatcaa taaaaaaaag 1200 gtgaactttg gatcaaataa tataaagtta tttcaaatta accaatgttg taaaatttgg 1260 atcttaaata tacagtcaca ttaactttgt aaacaatctt acgcatttga gcaaggttac 1320 ttccgctgaa agatgtatat aagatgaatt tgtttaaagt tttaaaagat aactttaaaa 1380 taaacatttt ttaagtaata aatatgggcc aatttgttta aacttgaaaa aataatttaa 1440 aaataagtac ttttttaaat aagtagatag aatttgtgtc agtttgttta attttattta 1500 ttgaaataaa tgcttatttt attaaaataa ataactttct atttttttaa tgtgttttcc 1560 taaattattt ctacttgttt taaaaagaaa atatttgcaa aaagacattt atataaaaaa 1620 attattttta agttttttaa aaaaatttaa acaaactcac cgtttgtttc ttcaaataag 1680 cggatttttt taaaaataga aacagttaaa aaaaataaaa taaacataaa aataaatatt 1740 ttattaaaat aaatacttat tttaacaaat aagtttaaac aaattatcac atgatttact 1800 tcttaaaaaa acaaaaaaaa actgtttatt ttaagtaaaa aaagtctaga caaacacatg 1860 ataaaatcat aaaactactt attttatttt taaaaaaata cttattttaa aaatatgctt 1920 taaaataatt agctcataat ttgcatcaaa aatcacaact cacgaattgg gagtgaataa 1980 attaatagta attagcttgg ctctttttct tattttttac tccctatttt ccttttttat 2040 aaggcttact agactctttt gcatgaccag cgtctctggt caaaagaatt aataataata 2100 ataataataa aattcattaa aaatcacatt aaaagaatat taataatcac attaaaaaca 2160 tatttaaaat cacattaaag cacattaatg acattttcaa tagtactaag aactctttca 2220 attcttgaac caatattact cttatcttaa tggaaaatac tctacactta tccgaacgaa 2280 aaaactgcaa aagagaagaa gaaatgaatg gagtgacacg cacaaattaa tttcaagata 2340 ccagcactta gcaatttagc atttgtttgg tagccgtcat tcgttgcatt gcattgcatt 2400 tgcatttgca ttgcaccgtc aactcgcaaa atatttataa gttttatact acaccaaacc 2460 aaaaaatatg ctgcgtctct ttctctctcc cctctctagc aattacacct cttttgccct 2520 ccattaaatg caattgcacc ctctcaatac gctctgcaat tactcttcgc tgccacattt 2580 tcctccatct gttccccact tctctctctc cttcaatgcc cttgtccctc tctcttcttc 2640 ttcttcttct tcttctcact ttctctctgt aacaccttct cttcatgata tagcttcttc 2700 accttttttt tctttctttt ctttgttacc atatataagt aaactagatt ccataactgt 2760 cccaagccct tccttcaaaa gggtcctctc ttttcaaccc aaaacagatc tacaccttca 2820 tcttccacca ccactctcac tggtaagtgc taaaaaatat tgataataaa agcttctgtc 2880 gcaaccaggg tcgcggcgtg tttgtgtgga acattcatgt tttgttaagg ttctctaagg 2940 tcacatttgt tttttttttt ttttaaacga aactaatgaa gctgtttggt acattttgtg 3000 agttctacta acattttccg ggtgagtcgc ttgcagctag ggctgtgaga aaaggaacgt 3060 tattaggtct gtaagaaaaa agaaaatatc ctgaaatata ggatgcgttg taggattctc 3120 cgagatcttc gtgttgtttt attttataaa taaaaaagtt ttgattttgt tttgtgaaaa 3180 ggcaaaagat cagataaggg ttacaatatt tcttttcttt tttattctgt ttacatatat 3240 tgatgatata tatatgcata gcgtatattt gtttcgaaat tttgataagt ttttatcttt 3300 ttaatttttt ttcggccgtt tgtataactg agttttgtag taacacactg actcgctttg 3360 acattgtcat ctgacagaat ccaagggctc aacctcttaa gctttttctc tatccaagaa 3420 cctcttaaga gagagaaaga ataagagaaa gagaaagtga gagagccaat tctacatttg 3480 ttggtggtgg tttttgaggc atgtgaaata atccaagggc tcaacctctt aagctttttc 3540 tctatccaag aacctcttaa gagagagaaa gaataagaga aagagaaagt gagagagcca 3600 attctacatt tgttggtggt ggtttttgag gcatgtgaaa t 3641 <210> 42 <211> 3509 <212> DNA <213> Glycine max <400> 42 tatatcattt gtttttaatg aagttatttt tggtttagtt tcttttaatg aagttactat 60 aaatttaatt atagttgttc aaactaatat ttagtccgaa caaaatacaa attggctacc 120 gctatgcgct atctaacaaa attttccgag tcataacatg cagatgcatg cggttcaaaa 180 ttaatacttg cactcatcat aaaatttgtt catatatagg taagactttc cacgtcaaat 240 ataaattaaa gtgcatagaa caaaaataat taaattgcaa atgtatttta tggtaaattg 300 caattcttta taataatgta ctatacaata tttatttaga aaacaaaatc ttgcgttacg 360 aattaatttt tttaatgtag ttacgaattg aattacacgg ttacaagtac catgcatttt 420 tatatgctaa gagaaattca tatttttaat caatctttta cgaagtgact agcttacacg 480 gttacactac attgtaatca aattatatta tatgtagtat cttttttctt ttataaaata 540 tattactagt aagtatcttg tgcggatact tttaaacaca aagaaaatat aaaaaataca 600 aaaatatata attttaaaaa tataaaaata taatcaacag aatgattttt cttaataatt 660 tgaaaatatt ttatcatgca taatgttaat aaaagaaact caagtattaa ttaaagaaat 720 taaaaaacta agaaccttct ttaatatgat taattgagga aatatatcga tttaacacat 780 ttcaagaaat aaaaaataca tgcttatgta aaatattaaa aatatatata aatgttatat 840 ctccgttaaa aacacgttta aatttagagt tcaattaaaa tatattgaaa atgttttttt 900 ttgtagtatt atctaatact gtataaatta taccatgtaa gataatattc ttgattcttg 960 taagaattac tttaaaaata tatctaaacg gtttgtaatc atgcaatatt ataaattttc 1020 ttatattccg taaaaaaata atattttatt ataataatat ataaaatcaa actcttatat 1080 tcataatata ggaaatagta gtcttcaaaa aaacaggaac gaataaatca tctcaatctt 1140 atcatatcaa ttattaatta cctaatttct ttaattaatt aaattatcaa taaaaaaaag 1200 gtgaactttg gatcaaataa tataaagtta tttcaaatta accaatgttg taaaatttgg 1260 atcttaaata tacagtcaca ttaactttgt aaacaatctt acgcatttga gcaaggttac 1320 ttccgctgaa agatgtatat aagatgaatt tgtttaaagt tttaaaagat aactttaaaa 1380 taaacatttt ttaagtaata aatatgggcc aatttgttta aacttgaaaa aataatttaa 1440 aaataagtac ttttttaaat aagtagatag aatttgtgtc agtttgttta attttattta 1500 ttgaaataaa tgcttatttt attaaaataa ataactttct atttttttaa tgtgttttcc 1560 taaattattt ctacttgttt taaaaagaaa atatttgcaa aaagacattt atataaaaaa 1620 attattttta agttttttaa aaaaatttaa acaaactcac cgtttgtttc ttcaaataag 1680 cggatttttt taaaaataga aacagttaaa aaaaataaaa taaacataaa aataaatatt 1740 ttattaaaat aaatacttat tttaacaaat aagtttaaac aaattatcac atgatttact 1800 tcttaaaaaa acaaaaaaaa actgtttatt ttaagtaaaa aaagtctaga caaacacatg 1860 ataaaatcat aaaactactt attttatttt taaaaaaata cttattttaa aaatatgctt 1920 taaaataatt agctcataat ttgcatcaaa aatcacaact cacgaattgg gagtgaataa 1980 attaatagta attagcttgg ctctttttct tattttttac tccctatttt ccttttttat 2040 aaggcttact agactctttt gcatgaccag cgtctctggt caaaagaatt aataataata 2100 ataataataa aattcattaa aaatcacatt aaaagaatat taataatcac attaaaaaca 2160 tatttaaaat cacattaaag cacattaatg acattttcaa tagtactaag aactctttca 2220 attcttgaac caatattact cttatcttaa tggaaaatac tctacactta tccgaacgaa 2280 aaaactgcaa aagagaagaa gaaatgaatg gagtgacacg cacaaattaa tttcaagata 2340 ccagcactta gcaatttagc atttgtttgg tagccgtcat tcgttgcatt gcattgcatt 2400 tgcatttgca ttgcaccgtc aactcgcaaa atatttataa gttttatact acaccaaacc 2460 aaaaaatatg ctgcgtctct ttctctctcc cctctctagc aattacacct cttttgccct 2520 ccattaaatg caattgcacc ctctcaatac gctctgcaat tactcttcgc tgccacattt 2580 tcctccatct gttccccact tctctctctc cttcaatgcc cttgtccctc tctcttcttc 2640 ttcttcttct tcttctcact ttctctctgt aacaccttct cttcatgata tagcttcttc 2700 accttttttt tctttctttt ctttgttacc atatataagt aaactagatt ccataactgt 2760 cccaagccct tccttcaaaa gggtcctctc ttttcaaccc aaaacagatc tacaccttca 2820 tcttccacca ccactctcac tggtaagtgc taaaaaatat tgataataaa agcttctgtc 2880 gcaaccaggg tcgcggcgtg tttgtgtgga acattcatgt tttgttaagg ttctctaagg 2940 tcacatttgt tttttttttt ttttaaacga aactaatgaa gctgtttggt acattttgtg 3000 agttctacta acattttccg ggtgagtcgc ttgcagctag ggctgtgaga aaaggaacgt 3060 tattaggtct gtaagaaaaa agaaaatatc ctgaaatata ggatgcgttg taggattctc 3120 cgagatcttc gtgttgtttt attttataaa taaaaaagtt ttgattttgt tttgtgaaaa 3180 ggcaaaagat cagataaggg ttacaatatt tcttttcttt tttattctgt ttacatatat 3240 tgatgatata tatatgcata gcgtatattt gtttcgaaat tttgataagt ttttatcttt 3300 ttaatttttt ttcggccgtt tgtataactg agttttgtag taacacactg actcgctttg 3360 acattgtcat ctgacagaat ccaagggctc aacctcttaa gctttttctc tatccaagaa 3420 cctcttaaga gagagaaaga ataagagaaa gagaaagtga gagagccaat tctacatttg 3480 ttggtggtgg tttttgaggc atgtgaaat 3509 <210> 43 <211> 3000 <212> DNA <213> Sorghum bicolor <400> 43 ttcactactt tattattttc tgagcaggaa aaagttatgt ttcctttgca aaaaatggga 60 cccacacgca cggcaagaag ccgtgccgtg ccgtgccgcg gcatggaaac agtactacac 120 tccaggactg catgcactcc gttgcagtga accatgcatc gatctcttcg cccgttcctc 180 tggaaactca taactttttt ttttaaagag agaaacccct caaacaaatt aaattggatc 240 atttgcacgg atttcatctt gccggtcgtc tgttgtgtat actgcaaatg agaattacgt 300 attttgtgga agtccttcgt cttgtttttt tatccttggc cttgtttagt ttcgaaaaat 360 ttcggatttc gctattataa cactttagtt tttatttgac aaatattgtc taatcatgaa 420 ctaactatga tcaaaagatt tatctcgcga tttacaaaca aattgtgtaa ttagttttta 480 tttttgttta tatttaatga tttatgcatt tgccgcaaga ttcgatgtga cggagaatct 540 tgaaaacttt ttggatctct gagggaacta aacaacgccc ttgtctaaca catgtatccc 600 caatttcatc ttgacggagg gatataacga tcgagtcaaa cctctgcatg gatgggtgca 660 ctattggcgt gtagcagtat atggcgccag gaaaaagtta aaactaaaga aatggatctt 720 ttaacaagta ctccctccgt cagcaacatg ccggagctgt gtttaaagtt agttttaatg 780 aagttttatt aaagttttat acatattaaa tatgctgatg taacattgta ttgatgaaga 840 gagatgataa aaattttatg agataaagtt ttattctaat aaaactcttc tgcactattt 900 ctaaatatga ctgcgttaaa aattgagtca tgaaatccgc cgtcgaggat ggcctaaagg 960 gcactcacaa tgcagactct atcatagagt ctaaggttat ttattacctc gaacaatgtg 1020 gacttagagt ctaaataaga cttggagtct tattttttct atctttttct tcaataaata 1080 taatgacata tcaacaaaat atcataaata atatgtaatt aattgtctta gactctatga 1140 tagagttttg cattgtgagg ctagtctcaa tgcatgtttc atgagagtgt catgcacatt 1200 aaatagggtg tcacataagc aaaattgctg acttggcagg gtcattaaat gaaggagttt 1260 catcagatga gagagaagtt tcatcctcat gaaactcata tggctcgatt atctagttta 1320 tagtcttggt aactgtacca tgaaactata cattgacact ggctgagtgt cctagcatgg 1380 ctcttatcct ttggccagaa ctgaagagac gctatgtata tccatggcta tctggaactt 1440 tcctctctcc atcactcagt aattacgccg gttatctaat ctgaaacatt tgtcaatgta 1500 ctgagtattc agcagaaaac gtaccacctg ccgaagtact atatcgtagc cgcgattaat 1560 tgactaataa gacaattgaa aaatagactg atattaccct cttagcctat aaaagaatgt 1620 aactatgaaa ccggtcaaac taatttaaat ttaattagat ttatagtaaa taatattagt 1680 atttatatct ttaaataaat ttataataaa actgtattct ataacaaaca tatattctat 1740 aactaattta ataatattta ttttatataa taatagataa cattttttat ataaatttag 1800 tcaaagttta aacgatcgat ctcgttactt aaaaaataag gagtagttag tattttttta 1860 ttcgagttac agtactaaat gatatataag tctatctcta catggaaatg catgatcatc 1920 gacttgccag cctttatttt ctcctctcct tcatgatgac tatacatagc tcgaaaataa 1980 attaatatat atgtcatgca gatacatgaa cgttcatttt cacattcatt tgccaaaaaa 2040 actacatatg acgacgtcca ataaatccaa aacacatgac atatcgtgca tgtttctcct 2100 tatatacaag tactatacca ggccttgttt agttccaatt ttttttgcaa aatagaaata 2160 gtagcacttt cgtttgtatt tgataaatat tgtctaatca tgaactaact agtctcaaaa 2220 tattcgtctc gtcaatttcg tccaaactgt gcaattagtt tttattttcg tctatattta 2280 atacttcatg catgcgtcta aagatttgat gtgatggaga atctgaaaaa ttttgcaaaa 2340 ttttttggga actaaacaag gccccaatat acttgtagaa atagaaatat ataataataa 2400 aaaatcatgc atccgtctct ctctcgctct gctagcaatt acgatacggc ctctcaataa 2460 atgcggattg caccacctca acactcgtcc gcaataactc cttcctccct tctccacatt 2520 ctcttccacc tccgtccgtt cccctcccct tcctcttcct ctcctccccc ttccatggcc 2580 gccgcttagc ttcgttctca aggcctcctc cctctcgcca cacacctcct gctagctacc 2640 cttctgagct tgcaaagttt tgccgcatca catcgatctt gagagctgca gcaggcagag 2700 atcgatcaga gttctatttt tgcagaggca cacaaacata tatagttcct gtatctgcta 2760 gatctcactg ccagccagct cgcagtgaag cttctctggg ctaccttgtc gctagatctg 2820 ttgctggtcg tgctgttact tggcaggcaa ggtatacatc tccatatagg aataggattg 2880 gcgccgcctg ttttcttggg ttgtgaggga gatatactac gcatgtgcca gtgaataaat 2940 gggaagcagc tagtgaggag gcaataagaa tcagggtttg agaggtcgcg agaggagaaa 3000 <210> 44 <211> 2500 <212> DNA <213> Sorghum bicolor <400> 44 ttcactactt tattattttc tgagcaggaa aaagttatgt ttcctttgca aaaaatggga 60 cccacacgca cggcaagaag ccgtgccgtg ccgtgccgcg gcatggaaac agtactacac 120 tccaggactg catgcactcc gttgcagtga accatgcatc gatctcttcg cccgttcctc 180 tggaaactca taactttttt ttttaaagag agaaacccct caaacaaatt aaattggatc 240 atttgcacgg atttcatctt gccggtcgtc tgttgtgtat actgcaaatg agaattacgt 300 attttgtgga agtccttcgt cttgtttttt tatccttggc cttgtttagt ttcgaaaaat 360 ttcggatttc gctattataa cactttagtt tttatttgac aaatattgtc taatcatgaa 420 ctaactatga tcaaaagatt tatctcgcga tttacaaaca aattgtgtaa ttagttttta 480 tttttgttta tatttaatga tttatgcatt tgccgcaaga ttcgatgtga cggagaatct 540 tgaaaacttt ttggatctct gagggaacta aacaacgccc ttgtctaaca catgtatccc 600 caatttcatc ttgacggagg gatataacga tcgagtcaaa cctctgcatg gatgggtgca 660 ctattggcgt gtagcagtat atggcgccag gaaaaagtta aaactaaaga aatggatctt 720 ttaacaagta ctccctccgt cagcaacatg ccggagctgt gtttaaagtt agttttaatg 780 aagttttatt aaagttttat acatattaaa tatgctgatg taacattgta ttgatgaaga 840 gagatgataa aaattttatg agataaagtt ttattctaat aaaactcttc tgcactattt 900 ctaaatatga ctgcgttaaa aattgagtca tgaaatccgc cgtcgaggat ggcctaaagg 960 gcactcacaa tgcagactct atcatagagt ctaaggttat ttattacctc gaacaatgtg 1020 gacttagagt ctaaataaga cttggagtct tattttttct atctttttct tcaataaata 1080 taatgacata tcaacaaaat atcataaata atatgtaatt aattgtctta gactctatga 1140 tagagttttg cattgtgagg ctagtctcaa tgcatgtttc atgagagtgt catgcacatt 1200 aaatagggtg tcacataagc aaaattgctg acttggcagg gtcattaaat gaaggagttt 1260 catcagatga gagagaagtt tcatcctcat gaaactcata tggctcgatt atctagttta 1320 tagtcttggt aactgtacca tgaaactata cattgacact ggctgagtgt cctagcatgg 1380 ctcttatcct ttggccagaa ctgaagagac gctatgtata tccatggcta tctggaactt 1440 tcctctctcc atcactcagt aattacgccg gttatctaat ctgaaacatt tgtcaatgta 1500 ctgagtattc agcagaaaac gtaccacctg ccgaagtact atatcgtagc cgcgattaat 1560 tgactaataa gacaattgaa aaatagactg atattaccct cttagcctat aaaagaatgt 1620 aactatgaaa ccggtcaaac taatttaaat ttaattagat ttatagtaaa taatattagt 1680 atttatatct ttaaataaat ttataataaa actgtattct ataacaaaca tatattctat 1740 aactaattta ataatattta ttttatataa taatagataa cattttttat ataaatttag 1800 tcaaagttta aacgatcgat ctcgttactt aaaaaataag gagtagttag tattttttta 1860 ttcgagttac agtactaaat gatatataag tctatctcta catggaaatg catgatcatc 1920 gacttgccag cctttatttt ctcctctcct tcatgatgac tatacatagc tcgaaaataa 1980 attaatatat atgtcatgca gatacatgaa cgttcatttt cacattcatt tgccaaaaaa 2040 actacatatg acgacgtcca ataaatccaa aacacatgac atatcgtgca tgtttctcct 2100 tatatacaag tactatacca ggccttgttt agttccaatt ttttttgcaa aatagaaata 2160 gtagcacttt cgtttgtatt tgataaatat tgtctaatca tgaactaact agtctcaaaa 2220 tattcgtctc gtcaatttcg tccaaactgt gcaattagtt tttattttcg tctatattta 2280 atacttcatg catgcgtcta aagatttgat gtgatggaga atctgaaaaa ttttgcaaaa 2340 ttttttggga actaaacaag gccccaatat acttgtagaa atagaaatat ataataataa 2400 aaaatcatgc atccgtctct ctctcgctct gctagcaatt acgatacggc ctctcaataa 2460 atgcggattg caccacctca acactcgtcc gcaataactc 2500 <210> 45 <211> 1204 <212> DNA <213> Arabidopsis thaliana <400> 45 atatcggttg ttgacgatta accatgcaaa aaagaaacat taattgcgaa tgtaaataac 60 aaaacatgta actcttgtag atatacatgt atcgacattt aaacccgaat atatatgtat 120 acctataatt tctctgattt tcacgctacc tgccacgtac atgggtgata ggtccaaact 180 cacaagtaaa agtttacgta cagtgaattc gtctttttgg gtataaacgt acatttaatt 240 tacacgtaag aaaggattac caattctttc atttatggta ccagacagag ttaaggcaaa 300 caagagaaac atatagagtt ttgatatgtt ttcttggata aatattaaat tgatgcaata 360 tttagggatg gacacaaggt aatatatgcc ttttaaggta tatgtgctat atgaatcgtt 420 tcgcatgggt actaaaatta tttgtcctta ctttatataa acaaattcca acaaaatcaa 480 gtttttgcta aaactagttt atttgcgggt tatttaatta cctatcatat tacttgtaat 540 atcattcgta tgttaacggg taaaccaaac caaaccggat attgaactat taaaaatctt 600 gtaaatttga cacaaactaa tgaatatcta aattatgtta ctgctatgat aacgaccatt 660 tttgtttttg agaaccataa tataaattac aggtacgtga caagtactaa gtatttatat 720 ccacctttag tcacagtacc aatattgcgc ctaccgggca acgtgaacgt gatcatcaaa 780 tcaaagtagt taccaaacgc tttgatctcg ataaaactaa aagctgacac gtcttgctgt 840 ttcttaattt atttctctta caacgacaat tttgagaaat atgaaatttt tatatcgaaa 900 gggaacagtc cttatcattt gctcccatca cttgcttttg tctagttaca actggaaatc 960 gaagagaagt attacaaaaa catttttctc gtcatttata aaaaaatgac aaaaaattaa 1020 atagagagca aagcaagagc gttgggtgac gttggtctct tcattaactc ctctcatcta 1080 ccccttcctc tgttcgcctt tatatccttc accttccctc tctcatcttc attaactcat 1140 cttcaaaaat accctaatca cattttgtaa caataataca attatacatt aaaactctcc 1200 gacg 1204 <210> 46 <211> 2605 <212> DNA <213> Arabidopsis thaliana <400> 46 tgaccaaatt ctagaagtga cttgataaca tgagaggcta taaagctggt tcctccggta 60 accaagtact ctgccatttt attaggctcc tctgattttg tgtaacattg acaaactaga 120 tcatgcgatt agatggtcta attttatttt attttgtgtt agaaggatgt tcaatcctgg 180 ttgatttagt ttatgtttga ctttttttta tgtagtcttt gaataatagt agttacatct 240 aactttcata aagacttgga ttagtataac ctaattataa gctttgggct tgaagcaaat 300 ataatgaact gaatgtgggc ttttaaagta tccaaagagg tttatgaaat gtgatatatt 360 ttttttggtc ttcaatctct tgaaaattgg ttagcaatcc attggcagtt gaaggctctc 420 tcagagacct tgactagaag gcctcaaagg caaaggaagg tggaaggaaa aatcaaaatt 480 cccggagctg aaagttgaca caaaggaatc aacctagttc tttcttgtct tagctttttt 540 gaagggaaat gaaaattgac acaaaagaag gtggaaggaa aaatatgatg cttgtccttt 600 attggacata tcattttgca gaatatatat tcttcctagc attttgcatg cattgtgtct 660 aggttgtcat gttgtggctg gtttactaag ttcctagtac aatgaacaaa gcgccagagc 720 tgaaagttga aataacaatc gtcaacatcc caaccgcctt aacattaaaa ccaaccaaaa 780 aaacaaaaac acatcccaac cgaaagcagc aatccaggaa gattagttta tgtaaatgac 840 aaacataaat atgaaaccgt cacatagttg agagtcgccg tgaatattta ctttttaaat 900 aaaaagatat ggttagccgc tggttggtct gacaccccat gtatcattat actcattatc 960 cgtatgaagg accatttttt catataagga tatacacgtt tgtaacgtat gcagagccaa 1020 caccaagtat ataacttgta ctgtcgtctc tgattctgtt aaagcggtta actagattat 1080 tgactatgat aattcaatgt aacaccaatt tatgccccgg acaaaaaaaa aatggtgaaa 1140 ccaaaatatt tgatttgcat cccagttact atttccctgc aatgatatgg gggcggcggt 1200 ttggagatat cgccaatgcc acaatttgtc gtaaaaacag ttaaacattt tactcactag 1260 tcactcccca caaaaaaaac gatacttagt tccactctct actactacaa aacttgtata 1320 cattaaaaaa cgatttttat tgagtagaca ttaatgatcg gcttataagt ctaaacaagt 1380 aatgtgacag aattcacgtg aacaaatgct cgagtgggat gtttttattg cttctcaagt 1440 tggtgatctt taatatcgcc aagattaggt tgtgcattaa atttggatta ctttttataa 1500 attaaatcaa gatatggata tgataaactt tctctctttt cttttagtta acatacttta 1560 tagactttat actacattag attaattgca gatttaaaca atgtttaaaa ttacaaaaac 1620 gttaaatgtc atattttcca tataaatttt gttttacgta aaagcaattt acatgaattt 1680 tgataaaatc atgaaaaaac agaagcggtg tccaagattg gggatgaatg agaaaacacc 1740 gcactgatag taactgaaaa gaatgtaacc agtctcacga tggggatggc ggagagaaag 1800 agagagacaa gacagtagag aaaagaaggt acgacggaga gatgaaggtt ggacagagag 1860 aacaaaacga gaacggcaat ggccgggaaa aaaggagagt agggaggaaa aaaaaaagaa 1920 ttttgttttt ttagcttttg tagggttttt tgttagtcgt aaaatataaa ttataatatc 1980 aaagaaaata atgagttaat tttaaagaag gttagatgat atttttcaca agtttttgtt 2040 gatatagtaa acccacaatt ttattttgtc attcaaagta aattttcaac aaaatatagc 2100 tgttgctggt cacattttaa cgggaaaaaa taattcttgt acctggatgt ataacctctg 2160 aaccttgtat tacaaattta caaaacacat tgatctcaat cagaccgaac atgtatatcg 2220 agtcaactga caaatctctt ttcctactct tgttacatat tctttctagg aaaaaaaaat 2280 gctttcaggc tcaatgaaat gaattaaaac agcaaaaggt tggtaaaagt ctttttcccc 2340 ataatcattt gctccataat tgacgtttgt ctggcaacaa ctggaaacac agatatagaa 2400 aaaaagattg gacagtaaat cataaaaaaa aaaaagcaaa attaaataga aagacgaagc 2460 gagagaaaga gcgttgagtg gcgtgagagt ttgagtccga aaattaactc ctctcccatc 2520 tgccaacttt cctctgtttc tcctttatat cccaaaccta ttcttcctcc acacctcatc 2580 ataggcatag caaaactctt ccaaa 2605 <210> 47 <211> 2500 <212> DNA <213> Arabidopsis thaliana <400> 47 tgaccaaatt ctagaagtga cttgataaca tgagaggcta taaagctggt tcctccggta 60 accaagtact ctgccatttt attaggctcc tctgattttg tgtaacattg acaaactaga 120 tcatgcgatt agatggtcta attttatttt attttgtgtt agaaggatgt tcaatcctgg 180 ttgatttagt ttatgtttga ctttttttta tgtagtcttt gaataatagt agttacatct 240 aactttcata aagacttgga ttagtataac ctaattataa gctttgggct tgaagcaaat 300 ataatgaact gaatgtgggc ttttaaagta tccaaagagg tttatgaaat gtgatatatt 360 ttttttggtc ttcaatctct tgaaaattgg ttagcaatcc attggcagtt gaaggctctc 420 tcagagacct tgactagaag gcctcaaagg caaaggaagg tggaaggaaa aatcaaaatt 480 cccggagctg aaagttgaca caaaggaatc aacctagttc tttcttgtct tagctttttt 540 gaagggaaat gaaaattgac acaaaagaag gtggaaggaa aaatatgatg cttgtccttt 600 attggacata tcattttgca gaatatatat tcttcctagc attttgcatg cattgtgtct 660 aggttgtcat gttgtggctg gtttactaag ttcctagtac aatgaacaaa gcgccagagc 720 tgaaagttga aataacaatc gtcaacatcc caaccgcctt aacattaaaa ccaaccaaaa 780 aaacaaaaac acatcccaac cgaaagcagc aatccaggaa gattagttta tgtaaatgac 840 aaacataaat atgaaaccgt cacatagttg agagtcgccg tgaatattta ctttttaaat 900 aaaaagatat ggttagccgc tggttggtct gacaccccat gtatcattat actcattatc 960 cgtatgaagg accatttttt catataagga tatacacgtt tgtaacgtat gcagagccaa 1020 caccaagtat ataacttgta ctgtcgtctc tgattctgtt aaagcggtta actagattat 1080 tgactatgat aattcaatgt aacaccaatt tatgccccgg acaaaaaaaa aatggtgaaa 1140 ccaaaatatt tgatttgcat cccagttact atttccctgc aatgatatgg gggcggcggt 1200 ttggagatat cgccaatgcc acaatttgtc gtaaaaacag ttaaacattt tactcactag 1260 tcactcccca caaaaaaaac gatacttagt tccactctct actactacaa aacttgtata 1320 cattaaaaaa cgatttttat tgagtagaca ttaatgatcg gcttataagt ctaaacaagt 1380 aatgtgacag aattcacgtg aacaaatgct cgagtgggat gtttttattg cttctcaagt 1440 tggtgatctt taatatcgcc aagattaggt tgtgcattaa atttggatta ctttttataa 1500 attaaatcaa gatatggata tgataaactt tctctctttt cttttagtta acatacttta 1560 tagactttat actacattag attaattgca gatttaaaca atgtttaaaa ttacaaaaac 1620 gttaaatgtc atattttcca tataaatttt gttttacgta aaagcaattt acatgaattt 1680 tgataaaatc atgaaaaaac agaagcggtg tccaagattg gggatgaatg agaaaacacc 1740 gcactgatag taactgaaaa gaatgtaacc agtctcacga tggggatggc ggagagaaag 1800 agagagacaa gacagtagag aaaagaaggt acgacggaga gatgaaggtt ggacagagag 1860 aacaaaacga gaacggcaat ggccgggaaa aaaggagagt agggaggaaa aaaaaaagaa 1920 ttttgttttt ttagcttttg tagggttttt tgttagtcgt aaaatataaa ttataatatc 1980 aaagaaaata atgagttaat tttaaagaag gttagatgat atttttcaca agtttttgtt 2040 gatatagtaa acccacaatt ttattttgtc attcaaagta aattttcaac aaaatatagc 2100 tgttgctggt cacattttaa cgggaaaaaa taattcttgt acctggatgt ataacctctg 2160 aaccttgtat tacaaattta caaaacacat tgatctcaat cagaccgaac atgtatatcg 2220 agtcaactga caaatctctt ttcctactct tgttacatat tctttctagg aaaaaaaaat 2280 gctttcaggc tcaatgaaat gaattaaaac agcaaaaggt tggtaaaagt ctttttcccc 2340 ataatcattt gctccataat tgacgtttgt ctggcaacaa ctggaaacac agatatagaa 2400 aaaaagattg gacagtaaat cataaaaaaa aaaaagcaaa attaaataga aagacgaagc 2460 gagagaaaga gcgttgagtg gcgtgagagt ttgagtccga 2500 <210> 48 <211> 3455 <212> DNA <213> Glycine max <400> 48 gagtatttat tgagagaata cttgcgagat tctttttaat atgttattta ctaattatta 60 ttatttaaaa tttattagaa atatttttta aatctcattt cttctattaa ttttaatcaa 120 taaaaaagaa aaaatatatt aaaaagtatg ctacatatct catttgttaa aataagagtt 180 tattaactta ggagagtttt cataagttca caatttaatt tgattctgaa taaaaaaaaa 240 agcttataga atatttgtat aactaattaa gtctaaataa attttacata aattgtttca 300 aaccctctaa tatatgcact agctcctaac tatcttttat tatatttaat tgcaatttta 360 atgaaatcct cttttgtttc agaagcttat agaataatta ttttcattta ataaatggtt 420 tttatgattc tgatgtaata ttttaattat tttctctctc tctctctctc tctccctata 480 tatatatata tatatatata tatatatata tatatatata tatatatata tatatatata 540 tatatatatt ctcctaacaa acctttaagg atttatccac cgctgcatat gccaggctga 600 aaaacaatta tttcattttc gtgggtgtat atataaaaaa aacttgtgga ataagagagg 660 tgttgatcga tgagctgtaa taaccttcaa tcttgagctt caaacaaact caagttatgg 720 tcttccctat agctagctaa tattttttgg tgttgatagc tagctaatat tttggactgc 780 actgtttaca ctcaacagtt caatttacac caacttaacc aacaaatctt ccttcgattt 840 tctgctgccc attgaaactc tgcatgtcaa aatgctactt gtcacaaata aaaaaggttg 900 agaaaaagga taataaggga ataacaacaa tatatacaac atgagcatcc gtttactata 960 cgctgcttaa tgtcatgcac agtacttatc taagcaaatt tttttgacaa gatgtgaatg 1020 atgtaacaat aaacatttca tctctttcta taatatatac ttagttaggt tattatattc 1080 gtacaatcag cttctgtatc attttatttc cactctcttt tttatcttgt tttttttcat 1140 ttctcatcat atttaccaat tttttgataa aaaataacca tatttaccga tttttctctc 1200 tttaatttat ttttattttt tttatccctc tatgtttatc tttcttgcac agttgcactt 1260 gtaaataata cccttataac ttttttccta tatactttcg caattataat acgcggtttg 1320 tctgtccgtt gactgttgct atatgtagta tatagcagta cttatttatt tattttatca 1380 gtacagtact tatttatttc agtgtacgaa agcggttgtt tttattttca gatttaaatt 1440 tgaattcaac ctcgtcataa tccccaatcc aagaacaaag acaaaattta gaagggtacg 1500 gagaacttaa agtctcacaa atcctttttt aattccggat atccacaaat taaccggaag 1560 taggactaat tcctgaaaat attaaaatgt atttagttta gggaattaat tttttctata 1620 aatattttta atttaataca tatagatgtc gaaattatat taaacctcta attacaaaca 1680 tataatgata ttgagatata catgattgat caggagttaa aaatataaaa acaaaaaaag 1740 tgaatgaaga aaaaaaaaag aaaataaatt gtgagtttaa atttttccag tgatatttta 1800 aaaaaataat aataaattaa tatttatcgt cgataatatt agtatctctt actccccttg 1860 ctctataggc tcgaacttat tatgattgat ggttgtcatt catatgttcc actaataaaa 1920 ggagtaagat tgacaaatga ataactagtt aaggtgagtg cattaaatca gtaagataca 1980 atactcgtta taaattatta aatgaacgaa tagtcaatgc ttgactgttg acaacctttg 2040 aatagtcaat gttatctatt tatccttaga tggactttgg caacattgat gttaatccag 2100 agtggaatgt aaagttttaa cgcatagcat gtctaaaatg atatgtatgt agtgtagaat 2160 ctttatatgt gttatttttt tttataaaga tgaataatca ataataaagt ttatgactgt 2220 tttccattga tgttatgata aaattaaata aataaatcca aggcaatcac ttcaaagacg 2280 ttataattag acaatatcag cttcttcttc tttttttcta aaaatgataa ggataaataa 2340 gtatgaatta atcataacag cacatctttt tgtactaaaa gatgattcat tttaaaaagg 2400 taagcattac gttacgaata aatttgtttt catgatattt tgtttgtatg caacatattt 2460 ttcaatacaa aaatattttt atataattca aactaaacat aattttaaaa ttatttttta 2520 gctaacacaa taaaatttat ataattaatt taaaaaataa ttgtgaacaa aataaatcag 2580 taaataattt tttcattata agatagccta aaattatttt ttataagatt tgtgtaaaaa 2640 taatatataa taaaagaaaa tatgttaaaa actgagcaat gctattgggt acaaatagca 2700 taaacacaaa accaaacgac tagaaacgtg acttaatatt atttttaaaa aatatatata 2760 ttaattaact tttttcataa atataaaata ggttttggta gaaatcacag ttgatgcaaa 2820 accaattcgt acaagacagc attttccttt aaaaaaattg tgtatatttg cctcagccac 2880 acgaaagcct aattaataag ttatctcatt ttggaagtac gttgtatcta catgtgaacg 2940 tatttaaatg taactaaatc catgtttaat gaaactttcg gcgttgatct gacacatggt 3000 tttattttaa ttttttttcg tttggatcca tgagattgag gtgtgggtgc actttttttg 3060 gtttgtgttt tattatcatt gttataagat cacacagacg cacgtacgga gaggagagat 3120 atagaagtag ttgcaattgc aaagaaggcc gtgtcccaat ttccctttga aagcacaaat 3180 ttaatgatga aaggaagaga agaaataaaa gtatgtttac gggtgttagg gtttagttag 3240 atgtggaagg ggtggagtta tgaaaattaa taccaagtta agtgagcgtt gagtgacgtg 3300 aagtcttaca ttaatacctc tcatctgccc cgttcagacc ttagggtccc ttggccttta 3360 aaacctcacc acccctacac tgagttatta tcattcaagc accattcttc tcgatcttct 3420 ctttctcaac cgaaccaaat tacagattta acaag 3455 <210> 49 <211> 3000 <212> DNA <213> Glycine max <400> 49 gagtatttat tgagagaata cttgcgagat tctttttaat atgttattta ctaattatta 60 ttatttaaaa tttattagaa atatttttta aatctcattt cttctattaa ttttaatcaa 120 taaaaaagaa aaaatatatt aaaaagtatg ctacatatct catttgttaa aataagagtt 180 tattaactta ggagagtttt cataagttca caatttaatt tgattctgaa taaaaaaaaa 240 agcttataga atatttgtat aactaattaa gtctaaataa attttacata aattgtttca 300 aaccctctaa tatatgcact agctcctaac tatcttttat tatatttaat tgcaatttta 360 atgaaatcct cttttgtttc agaagcttat agaataatta ttttcattta ataaatggtt 420 tttatgattc tgatgtaata ttttaattat tttctctctc tctctctctc tctccctata 480 tatatatata tatatatata tatatatata tatatatata tatatatata tatatatata 540 tatatatatt ctcctaacaa acctttaagg atttatccac cgctgcatat gccaggctga 600 aaaacaatta tttcattttc gtgggtgtat atataaaaaa aacttgtgga ataagagagg 660 tgttgatcga tgagctgtaa taaccttcaa tcttgagctt caaacaaact caagttatgg 720 tcttccctat agctagctaa tattttttgg tgttgatagc tagctaatat tttggactgc 780 actgtttaca ctcaacagtt caatttacac caacttaacc aacaaatctt ccttcgattt 840 tctgctgccc attgaaactc tgcatgtcaa aatgctactt gtcacaaata aaaaaggttg 900 agaaaaagga taataaggga ataacaacaa tatatacaac atgagcatcc gtttactata 960 cgctgcttaa tgtcatgcac agtacttatc taagcaaatt tttttgacaa gatgtgaatg 1020 atgtaacaat aaacatttca tctctttcta taatatatac ttagttaggt tattatattc 1080 gtacaatcag cttctgtatc attttatttc cactctcttt tttatcttgt tttttttcat 1140 ttctcatcat atttaccaat tttttgataa aaaataacca tatttaccga tttttctctc 1200 tttaatttat ttttattttt tttatccctc tatgtttatc tttcttgcac agttgcactt 1260 gtaaataata cccttataac ttttttccta tatactttcg caattataat acgcggtttg 1320 tctgtccgtt gactgttgct atatgtagta tatagcagta cttatttatt tattttatca 1380 gtacagtact tatttatttc agtgtacgaa agcggttgtt tttattttca gatttaaatt 1440 tgaattcaac ctcgtcataa tccccaatcc aagaacaaag acaaaattta gaagggtacg 1500 gagaacttaa agtctcacaa atcctttttt aattccggat atccacaaat taaccggaag 1560 taggactaat tcctgaaaat attaaaatgt atttagttta gggaattaat tttttctata 1620 aatattttta atttaataca tatagatgtc gaaattatat taaacctcta attacaaaca 1680 tataatgata ttgagatata catgattgat caggagttaa aaatataaaa acaaaaaaag 1740 tgaatgaaga aaaaaaaaag aaaataaatt gtgagtttaa atttttccag tgatatttta 1800 aaaaaataat aataaattaa tatttatcgt cgataatatt agtatctctt actccccttg 1860 ctctataggc tcgaacttat tatgattgat ggttgtcatt catatgttcc actaataaaa 1920 ggagtaagat tgacaaatga ataactagtt aaggtgagtg cattaaatca gtaagataca 1980 atactcgtta taaattatta aatgaacgaa tagtcaatgc ttgactgttg acaacctttg 2040 aatagtcaat gttatctatt tatccttaga tggactttgg caacattgat gttaatccag 2100 agtggaatgt aaagttttaa cgcatagcat gtctaaaatg atatgtatgt agtgtagaat 2160 ctttatatgt gttatttttt tttataaaga tgaataatca ataataaagt ttatgactgt 2220 tttccattga tgttatgata aaattaaata aataaatcca aggcaatcac ttcaaagacg 2280 ttataattag acaatatcag cttcttcttc tttttttcta aaaatgataa ggataaataa 2340 gtatgaatta atcataacag cacatctttt tgtactaaaa gatgattcat tttaaaaagg 2400 taagcattac gttacgaata aatttgtttt catgatattt tgtttgtatg caacatattt 2460 ttcaatacaa aaatattttt atataattca aactaaacat aattttaaaa ttatttttta 2520 gctaacacaa taaaatttat ataattaatt taaaaaataa ttgtgaacaa aataaatcag 2580 taaataattt tttcattata agatagccta aaattatttt ttataagatt tgtgtaaaaa 2640 taatatataa taaaagaaaa tatgttaaaa actgagcaat gctattgggt acaaatagca 2700 taaacacaaa accaaacgac tagaaacgtg acttaatatt atttttaaaa aatatatata 2760 ttaattaact tttttcataa atataaaata ggttttggta gaaatcacag ttgatgcaaa 2820 accaattcgt acaagacagc attttccttt aaaaaaattg tgtatatttg cctcagccac 2880 acgaaagcct aattaataag ttatctcatt ttggaagtac gttgtatcta catgtgaacg 2940 tatttaaatg taactaaatc catgtttaat gaaactttcg gcgttgatct gacacatggt 3000 <210> 50 <211> 3138 <212> DNA <213> Populus trichocarpa <400> 50 aaaaaggaaa ttaactaata ttatatatat agtgaattct taaaaatacg tccctcaata 60 tgataaacat tgttatatcg atcgggaaat atagttttag tgttcctagt atttttaaaa 120 agaggtgcct tcgtccaact aatcaaaata cgagagtcaa actatgagag ttacatataa 180 tttggtgtca gagtagaatg gcatgaagtt atggctgcta aactttagct gactgtctgt 240 ttcatagcct aatttagcca acacctcaag ttcctctctc ctgatggcat ctgctgctat 300 ctctatcctc atagctttta acccatatca catgttttca aggtaccaga gaggcggtgg 360 cttgatctaa gaaatttaat cttaccggta atttgcaatt tatttgcata gttgatttca 420 attaatattg atgtcatact ataaccggac ttactaaata tattaccagg gtctaaaatg 480 cataccacca tcaccaggac cggaattaag ggccggctcc tgcaatgaaa ttttttccag 540 tatttttcat ttaataaatt agatttttag ttttaaaaat aaaaaataat tttttttaat 600 ttgaccagct caaaataaaa ttaatagctt cgccccttat cctcacctcg acatttgtct 660 ttgtaccagt taccaaagac atcacggcag gattcgaggc tggtgaagag agaagattaa 720 agaaaggtgt acctcaagga gggacgatga gtaaattaaa tatatttatt ttggaatttt 780 taaattcatg agcacacgag gcgcgacggc tcttattaaa tttttcgtac ttaccatgga 840 gaaaaaaaaa acaaataaag taataaatgt ataagcgaga cgtacctttt tggttcctgc 900 actacaactg tttttgaagc acaaaatatt attttttttc ctttcaacgt aaaacaccaa 960 ttcctttaaa aggaaacaac ttttcttcga atataaaaca ggggtttaaa gcaacgcaac 1020 tcgttgagat tctttcaagt ttgtgcagtg cagctcatga tggtagtcat aacacaacag 1080 tcctggatcc aactacattt ataattaagt tctctctttg gttgaccagt gagaatcatt 1140 gagaaatatc aaaacgagtt tttaaaaaaa tgaatatgaa aacaaatgct ccgctttgac 1200 ttttctttac ttttccctgc tttaatggat aggggtatca atttgtccag ttgcagttgt 1260 ggaagtaaaa tacaattatg taacatttta gttgtttttt aaaatgtttt taatttaaaa 1320 atatattaaa atatttttta ttttaaaaaa attattttta atactaacat attacaacca 1380 gatacaaaca tgaaaaatat tcttaaacaa ttctttttaa ttttttaata aaatattatt 1440 tgaaatacaa tactgtaaca ttttagttga aactctattt tctctacaag gatgcatgga 1500 tacttttttt tttaactaat tgagtgtata acagtaatta taatttttta aaatattttt 1560 tatttgaaaa tatattaaaa taatattttt tattttttaa taaattatct ttgacatcat 1620 tatattaaaa cgatttaaaa aaataaaatt taataaatca gtgtttgaaa tgcgtttaaa 1680 tatctctttg gcgcggtcag ccatagcttt ccatgcatta aactacaggc gaggacatca 1740 gacgccagtt gttatataat ttttggacat aaggtgacaa atcttaattt ccccaccaca 1800 tgcagtacta ttcttctttc ctttcgatct tgtgctaatt aactagtcac atgttttgct 1860 taattaaatt gaagtgtcaa tttcattttc atgttaaatt aatttgttgt cggttctata 1920 tataacaaaa ttatcccttc tgtttgtaag tattgaattt tttacaagat ttatttattt 1980 aaatacaaat tacttttatt tgaaatcttt taattatata tattcctttg ttattgaaca 2040 tgaatccaac atagattcat gggaattagg tccacgagaa tatattagcc cctagctata 2100 aacgactgag aagatacatt ttactaaccc ccctctcctc tagtggatgc gatcaggttc 2160 aagtaaaact gattacagtg atacaatttc aaatgcttct ggatctttct cccctttcac 2220 tcataattaa tttttctaat ccctcctttt tcttattata aaatgtatat gtcatcaaat 2280 ttgaaaatat gcatatacca aacatcatta aaaataacta ttttattttg taatcattcc 2340 tttgttgatt ttttagctca aaacaaacaa attctaaacg tgattactac gattcaactt 2400 caatacagat gttacttgaa attctgctag attacttaat taatagtttc tagctaaatt 2460 atccatatat atctcttgat ttataatttc tgattaataa aaacagtgat catttgattt 2520 ggagcacaca ataacaaata tgctattgat gagtgaaaaa aaatagccga gagaaatata 2580 aacaaagtta ttaaatttgg ttctatttag aactccacta gagattcaag tcaatcaaaa 2640 aaatgttatt ttaatttaat aaaaataaaa ctaaatttta atcagttcat taattaattc 2700 attgaattac tcatttgatt agttgcaatc tcaccaagtt ttttttttaa aatccgacac 2760 agcaataaca aaggatattg gacagggaag ggtactccga acccgtacct ggtaggatgt 2820 ctagttacac ccggagagaa aagaaaagaa aaggccagaa aaaattaata gatatatttt 2880 ttttttttgg tgtaagaaaa ttaatagagt taagtgggcg ttgagcgcct cattaatctt 2940 ctcatctgcc tccatcattg cccacggccc cgtgccttta tatctcccat cctcccgcct 3000 ccccccctca cctaatatca tcaactctct ttctctcaag caacaaccat acactcatct 3060 cttctcgtct ccctctctct ccccctcagt acactacatt cctcaacatt tcttcttctt 3120 cttcttcttg gcaaaata 3138 <210> 51 <211> 3000 <212> DNA <213> Populus trichocarpa <400> 51 aaaaaggaaa ttaactaata ttatatatat agtgaattct taaaaatacg tccctcaata 60 tgataaacat tgttatatcg atcgggaaat atagttttag tgttcctagt atttttaaaa 120 agaggtgcct tcgtccaact aatcaaaata cgagagtcaa actatgagag ttacatataa 180 tttggtgtca gagtagaatg gcatgaagtt atggctgcta aactttagct gactgtctgt 240 ttcatagcct aatttagcca acacctcaag ttcctctctc ctgatggcat ctgctgctat 300 ctctatcctc atagctttta acccatatca catgttttca aggtaccaga gaggcggtgg 360 cttgatctaa gaaatttaat cttaccggta atttgcaatt tatttgcata gttgatttca 420 attaatattg atgtcatact ataaccggac ttactaaata tattaccagg gtctaaaatg 480 cataccacca tcaccaggac cggaattaag ggccggctcc tgcaatgaaa ttttttccag 540 tatttttcat ttaataaatt agatttttag ttttaaaaat aaaaaataat tttttttaat 600 ttgaccagct caaaataaaa ttaatagctt cgccccttat cctcacctcg acatttgtct 660 ttgtaccagt taccaaagac atcacggcag gattcgaggc tggtgaagag agaagattaa 720 agaaaggtgt acctcaagga gggacgatga gtaaattaaa tatatttatt ttggaatttt 780 taaattcatg agcacacgag gcgcgacggc tcttattaaa tttttcgtac ttaccatgga 840 gaaaaaaaaa acaaataaag taataaatgt ataagcgaga cgtacctttt tggttcctgc 900 actacaactg tttttgaagc acaaaatatt attttttttc ctttcaacgt aaaacaccaa 960 ttcctttaaa aggaaacaac ttttcttcga atataaaaca ggggtttaaa gcaacgcaac 1020 tcgttgagat tctttcaagt ttgtgcagtg cagctcatga tggtagtcat aacacaacag 1080 tcctggatcc aactacattt ataattaagt tctctctttg gttgaccagt gagaatcatt 1140 gagaaatatc aaaacgagtt tttaaaaaaa tgaatatgaa aacaaatgct ccgctttgac 1200 ttttctttac ttttccctgc tttaatggat aggggtatca atttgtccag ttgcagttgt 1260 ggaagtaaaa tacaattatg taacatttta gttgtttttt aaaatgtttt taatttaaaa 1320 atatattaaa atatttttta ttttaaaaaa attattttta atactaacat attacaacca 1380 gatacaaaca tgaaaaatat tcttaaacaa ttctttttaa ttttttaata aaatattatt 1440 tgaaatacaa tactgtaaca ttttagttga aactctattt tctctacaag gatgcatgga 1500 tacttttttt tttaactaat tgagtgtata acagtaatta taatttttta aaatattttt 1560 tatttgaaaa tatattaaaa taatattttt tattttttaa taaattatct ttgacatcat 1620 tatattaaaa cgatttaaaa aaataaaatt taataaatca gtgtttgaaa tgcgtttaaa 1680 tatctctttg gcgcggtcag ccatagcttt ccatgcatta aactacaggc gaggacatca 1740 gacgccagtt gttatataat ttttggacat aaggtgacaa atcttaattt ccccaccaca 1800 tgcagtacta ttcttctttc ctttcgatct tgtgctaatt aactagtcac atgttttgct 1860 taattaaatt gaagtgtcaa tttcattttc atgttaaatt aatttgttgt cggttctata 1920 tataacaaaa ttatcccttc tgtttgtaag tattgaattt tttacaagat ttatttattt 1980 aaatacaaat tacttttatt tgaaatcttt taattatata tattcctttg ttattgaaca 2040 tgaatccaac atagattcat gggaattagg tccacgagaa tatattagcc cctagctata 2100 aacgactgag aagatacatt ttactaaccc ccctctcctc tagtggatgc gatcaggttc 2160 aagtaaaact gattacagtg atacaatttc aaatgcttct ggatctttct cccctttcac 2220 tcataattaa tttttctaat ccctcctttt tcttattata aaatgtatat gtcatcaaat 2280 ttgaaaatat gcatatacca aacatcatta aaaataacta ttttattttg taatcattcc 2340 tttgttgatt ttttagctca aaacaaacaa attctaaacg tgattactac gattcaactt 2400 caatacagat gttacttgaa attctgctag attacttaat taatagtttc tagctaaatt 2460 atccatatat atctcttgat ttataatttc tgattaataa aaacagtgat catttgattt 2520 ggagcacaca ataacaaata tgctattgat gagtgaaaaa aaatagccga gagaaatata 2580 aacaaagtta ttaaatttgg ttctatttag aactccacta gagattcaag tcaatcaaaa 2640 aaatgttatt ttaatttaat aaaaataaaa ctaaatttta atcagttcat taattaattc 2700 attgaattac tcatttgatt agttgcaatc tcaccaagtt ttttttttaa aatccgacac 2760 agcaataaca aaggatattg gacagggaag ggtactccga acccgtacct ggtaggatgt 2820 ctagttacac ccggagagaa aagaaaagaa aaggccagaa aaaattaata gatatatttt 2880 ttttttttgg tgtaagaaaa ttaatagagt taagtgggcg ttgagcgcct cattaatctt 2940 ctcatctgcc tccatcattg cccacggccc cgtgccttta tatctcccat cctcccgcct 3000 <210> 52 <211> 2556 <212> DNA <213> Vitus vinifera <400> 52 tttttaaaat aattatttca aaaaatatca acagtgcaat tattttttat aataaaattt 60 aacatatcat ataaaaagct ttcttttttt aataaaaaat tattaattag aaaattattt 120 tctgttttga aattaaaaaa aataaaaaat aaaaaaattc cagaagagtt ttgaaacagg 180 accacatgtt aaaggtaggt gcttaaatta aaaattgaat atgatatcga cggtggcagc 240 aggtggagtc tgagtgtgag tggggatggc cgttggccac ataaaattct cagagagtta 300 ggtttggttc ccatgaaatg aaagtggcct ttccatttaa cattctacaa ggtgtctcca 360 tttgcctttg ccaccattaa atttagggtt tctgggttca tcttctgtcc atttcagatg 420 aatgcactct cctctctccc ccgtatatcc cctctgcact tccatcttaa atgccatatt 480 ttgtggttca aaacttgacc cccattcata ctccaccttc atgtcttgat gctttcttat 540 ttttgcactc caaaagaagg gaaaaaagag agaagtatga aacaaaaacg aggcataaca 600 tgatcacttc attatataat tacccatcaa atatatattt actaaaatta caaatgtcaa 660 taacaaaata ttagatcgat aactgttttc aaaaacagtt tttgaaaata gtctttaaaa 720 attattattt catgttttta taaaataaaa ttttatttaa aaacttaaaa tgtttttaac 780 ttgtttttag tatctttaaa tatattttaa aaataatttt tatatgcctt tgtcatttca 840 ttttgatgtt aatttttata tttgaaaatt gttttaatta ttaattttaa tttaatgaaa 900 gctcagccca atctaaacca gcaagatggt tgatgataga taataattaa taatatatac 960 cccaaatcag aataaatctg ctagccgtta ccctcaaata tttgaagtat ccctatacat 1020 tttggtgatg caacttgttg tgttgattag gtttttagta tacatggttt aagagaaaca 1080 atccttaatt agtttatact aaatgatgtt atagacacta agtccacctt atgttagaag 1140 tactagtttt agattagctt aactcaaatg ttttatttca aaagattaga gtatgattaa 1200 gaatgttttt tgaaaaacta ttttataaaa tagttttttt aaatagaatt ttatttgaga 1260 atttgaaata tactccgact tgtttttatg tatttttaaa tattttttaa aattaatttt 1320 tatttattat gctttatttt tcatcatttt ttgtatttgt attgttaaaa caatttctag 1380 aacaagtaaa aataattaaa atatgtcatt taaaatcatt atattttcac aataagtttt 1440 ccaaatatta tttttattaa aaaataaaaa taataatttt ttgctcgtaa gtacatttgt 1500 ttttgtgttt tttttttctt caaagaactc tttatcatag tatcatgtaa gaggtatcat 1560 tacgtttttt ttccaagcac tcaatgctaa caataccaaa atgtttacta aaaatgtaat 1620 tttatttgtt ttttgtttta aaaaatagaa aattatttta aaaagtaatt acaaaaaacc 1680 tatttgacaa ctatttttaa aaacaattct gaaacttatt tttgtataac aaaactctat 1740 aatattttta atattcttat atatgtttta aaaataattt ttatatctag tatttaaaac 1800 aattctaaaa ataataataa aaaaaaatta aaattttttt tattttttgc ttttaataat 1860 aaaaaacaaa aaataatttt taatggttta gtttgttttt attatgaata atagaaaatt 1920 attttaaaaa ccaattctca gtacaaaatg gcccggaatg ttcggattgc gttgtacaaa 1980 acccatgata caatcacttt ctagtttgta gaggtgggat gaggctatta attgcttggg 2040 agtgtcattg aaaggatgga tcttggctta tgaaatttca ccactttgga agaaaaacaa 2100 tgaactgatg aattaattta agtcccaaat caattaatta aggatgaata ttttgaattt 2160 acaaggaagg gtgctatttt tttattagac caaagtagaa aaatgaacaa ataacagtaa 2220 cccattaaag aggggcatca tgaatatgaa aatgaaaatg aaagagaaaa agagaaacaa 2280 ggggggagtt tagttgatta ataatgaggg aggttaggaa ataaagtgta ggtgagtcca 2340 ggtgggggag cgttgcgtga gtgtgagctg ttcattaaat cctctcatct gccctctatt 2400 taattcccac tattttagct ttgcctttaa atcccacctc accctcccat tttctcccta 2460 ccactctctt ttctctcttc tttcatcaac atcatcagct ctttctcttt ctctctttct 2520 gtctttctcc ctcaattctt aattctcact cccaaa 2556 <210> 53 <211> 2500 <212> DNA <213> Vitus vinifera <400> 53 tttttaaaat aattatttca aaaaatatca acagtgcaat tattttttat aataaaattt 60 aacatatcat ataaaaagct ttcttttttt aataaaaaat tattaattag aaaattattt 120 tctgttttga aattaaaaaa aataaaaaat aaaaaaattc cagaagagtt ttgaaacagg 180 accacatgtt aaaggtaggt gcttaaatta aaaattgaat atgatatcga cggtggcagc 240 aggtggagtc tgagtgtgag tggggatggc cgttggccac ataaaattct cagagagtta 300 ggtttggttc ccatgaaatg aaagtggcct ttccatttaa cattctacaa ggtgtctcca 360 tttgcctttg ccaccattaa atttagggtt tctgggttca tcttctgtcc atttcagatg 420 aatgcactct cctctctccc ccgtatatcc cctctgcact tccatcttaa atgccatatt 480 ttgtggttca aaacttgacc cccattcata ctccaccttc atgtcttgat gctttcttat 540 ttttgcactc caaaagaagg gaaaaaagag agaagtatga aacaaaaacg aggcataaca 600 tgatcacttc attatataat tacccatcaa atatatattt actaaaatta caaatgtcaa 660 taacaaaata ttagatcgat aactgttttc aaaaacagtt tttgaaaata gtctttaaaa 720 attattattt catgttttta taaaataaaa ttttatttaa aaacttaaaa tgtttttaac 780 ttgtttttag tatctttaaa tatattttaa aaataatttt tatatgcctt tgtcatttca 840 ttttgatgtt aatttttata tttgaaaatt gttttaatta ttaattttaa tttaatgaaa 900 gctcagccca atctaaacca gcaagatggt tgatgataga taataattaa taatatatac 960 cccaaatcag aataaatctg ctagccgtta ccctcaaata tttgaagtat ccctatacat 1020 tttggtgatg caacttgttg tgttgattag gtttttagta tacatggttt aagagaaaca 1080 atccttaatt agtttatact aaatgatgtt atagacacta agtccacctt atgttagaag 1140 tactagtttt agattagctt aactcaaatg ttttatttca aaagattaga gtatgattaa 1200 gaatgttttt tgaaaaacta ttttataaaa tagttttttt aaatagaatt ttatttgaga 1260 atttgaaata tactccgact tgtttttatg tatttttaaa tattttttaa aattaatttt 1320 tatttattat gctttatttt tcatcatttt ttgtatttgt attgttaaaa caatttctag 1380 aacaagtaaa aataattaaa atatgtcatt taaaatcatt atattttcac aataagtttt 1440 ccaaatatta tttttattaa aaaataaaaa taataatttt ttgctcgtaa gtacatttgt 1500 ttttgtgttt tttttttctt caaagaactc tttatcatag tatcatgtaa gaggtatcat 1560 tacgtttttt ttccaagcac tcaatgctaa caataccaaa atgtttacta aaaatgtaat 1620 tttatttgtt ttttgtttta aaaaatagaa aattatttta aaaagtaatt acaaaaaacc 1680 tatttgacaa ctatttttaa aaacaattct gaaacttatt tttgtataac aaaactctat 1740 aatattttta atattcttat atatgtttta aaaataattt ttatatctag tatttaaaac 1800 aattctaaaa ataataataa aaaaaaatta aaattttttt tattttttgc ttttaataat 1860 aaaaaacaaa aaataatttt taatggttta gtttgttttt attatgaata atagaaaatt 1920 attttaaaaa ccaattctca gtacaaaatg gcccggaatg ttcggattgc gttgtacaaa 1980 acccatgata caatcacttt ctagtttgta gaggtgggat gaggctatta attgcttggg 2040 agtgtcattg aaaggatgga tcttggctta tgaaatttca ccactttgga agaaaaacaa 2100 tgaactgatg aattaattta agtcccaaat caattaatta aggatgaata ttttgaattt 2160 acaaggaagg gtgctatttt tttattagac caaagtagaa aaatgaacaa ataacagtaa 2220 cccattaaag aggggcatca tgaatatgaa aatgaaaatg aaagagaaaa agagaaacaa 2280 ggggggagtt tagttgatta ataatgaggg aggttaggaa ataaagtgta ggtgagtcca 2340 ggtgggggag cgttgcgtga gtgtgagctg ttcattaaat cctctcatct gccctctatt 2400 taattcccac tattttagct ttgcctttaa atcccacctc accctcccat tttctcccta 2460 ccactctctt ttctctcttc tttcatcaac atcatcagct 2500 <210> 54 <211> 3000 <212> DNA <213> Sorghum bicolor <400> 54 tatccccttg atgacgacga gcgttgagcg tgcgtggcag tgattgtctg attgactgat 60 cgagcaagtg tgtatagtag ccaactctct ctgcatgtgg tggcttgcag gacgcacaca 120 actattatag aggccggccg cgcatatata taggtatata ggatcagtca atcagacaat 180 cactaaagat acattgacat gtttgtgttt ttcctagaaa atcatttcaa aattttcaaa 240 ggaaagcctt ttaagacaaa ctagaagtta tttattttga tctatatgta tttttactcc 300 tacataagag gctaccttta tcttccacat aaaattttag aagataactt tgacatcatg 360 gacaagttcg aatagaccca tgacggctaa tgtgtgatgt gagtctcacc ttgggtcttg 420 agagttggtt agaccaacat actcccttca tcctaatcta taagtcactc taagaatctt 480 gtcaaaacat ctcaagtaac taacattata gaaaaaatca aaaagattta tgacatcaaa 540 taaatattac tataaaaata taactaatga agaatctaat gatacttaga tgatatcata 600 aatgttatta tgttatcata taaatttggt caaacttaag atacttttga ctttctaaga 660 ttcttggaat gacttataat ttaggatgga aggagtatac ggcttctaga gtccttatca 720 tcaacaccat gctagacctt ttacgtgagg ggaggatgaa agcataagtg ggttgatagg 780 actgtggttc gcttagtgta tggtgtgaac ccgatagact ctagaaactt gtgtatcaat 840 ggtcaattat gagtagaagg ttccatcgga acaattatta tttatttcaa gctatttcgg 900 ctctttctta atcttcaaaa agaaattaat tccgtaatgg taagacgaaa aaagaaaaaa 960 aaataaaaag gaagtgtgga aaaatgacaa aatggcggca cagggaggcg agaaccaccg 1020 cttttaggaa ataagcctac aatttgattc tcgtctcgtt ttacttgcat tttctttcgt 1080 tcagtctcgt tctgagagtg gaatcgaacc actcactccg tttggattta gagtccaaag 1140 ggcccagcca gcgaaagagg attgacctcc gtggacatgt ggtgcatggt acgtgttggc 1200 ccaatcaatg tagcttgtga catggcactg cgccattact agatgcatgc acgtgtgttt 1260 caagagggaa cattttttgt gaaatgtggt tgtattcaca tcaatccatg tgtattaggg 1320 tggattgaag tggaagttga actaaatttt acctcaatcc accgcaaccc atgtggattg 1380 aggcgaatcc aacaacattc aaataaggcc ttacttggtt atgggtgtgt tggggagacg 1440 ttgacatgag gccctttgag atggttgcaa cctgggtcaa tttaacttag gccatggtgg 1500 atcatgtgta tgttgaggtg agatcgtgca ggtttggtct caccttgatc actgagggtg 1560 gatatagcac atataatgtt tttaatctac atcattaact ctaggttggc ccttttatgt 1620 aaagcaagga tgaaagcata aataggttag tgaaagtgtg cgcttaacta agagtgtaga 1680 gtggatctga gatattgaac tctaagcact aaataaccaa gttttaactt atgtcaatgt 1740 taatttgtta gtataaacaa taagaatata ataagcatat aaatattgta atacaactta 1800 ccaccaataa ctttagtact ctactgcaaa gtataaagac gttttggctt ttctagatat 1860 atagtttggt tgtgcactta catatatttt atgtctagat atatacatat tagaatgtat 1920 ctagaaaaga caaactatct tacaatttag agtgaagaga gtatatatag agatgtaatg 1980 atcatcttgc atcattgaaa tactatatga catcaaatta ttacaaaaac aaaaatatca 2040 ttggttgaag cttgttgaag atcataaatt attatttttg agccctcata aggtacaact 2100 gccgtttcta aacatatgca caaacacaaa cactcacgca cgcatacaac acacacccag 2160 tgtgcacgac ctacaaacta tacacacgta gatacgaacc atgttacggt cgacgcggac 2220 atgaactggg ccttcatgag gggcagcact agatatcact gagtgatctc actgagtcca 2280 ccccttcccc ctcctcctga tggcatcctt ggaccggaaa ccatgtcagg gctctccgat 2340 ctttgcttca accatggcgt caagtttgaa gcactcgtct cacagcgttt tggatgccca 2400 gtgagtccca tctctcccca gactgttttg gatctttgct tcaaccatgg cgtcaagttt 2460 tggatctctc cccagcattt tggatgcccg gtccgctgtc cgtctgaata ttgattcagt 2520 gagtctcatc cttcaagctt gctttggtgg caaagaatac aatgttaccc atctctctgg 2580 atggatgttc aaattttcaa tatcttgcaa aaatgtggct ttcatgatta acaaccttaa 2640 aagccactct ttcaaaactt ttctcatctt ctttcacctt tggagtggag gaggaccaaa 2700 ttggcgcaag gattttactc tctggcaaca ggagcaagat gcaaaatgga accttgttgg 2760 atccaagtcc aagaaatctt atgctgatat tgctaaatca aatatcaaaa aatcagtttt 2820 gttgcgctta tcttacccca ccaattatgc atccaacttc ctggagcccc ctcgcagagc 2880 atctttggct cctgacaggt tctcccatcg ccctgctcaa aataatgtga tgaaagcaca 2940 aattgatagt aaacctgtca aaggtttgca acaatctcat cctagtggct ttccaagatg 3000 <210> 55 <211> 3070 <212> DNA <213> Zea mays <400> 55 cgggcggagc ggagttcgcc gtcttccggg gcctagccca agtccgagcc ctgggtcggg 60 cggaacggag ttcgccgtct tccggggctt agcccgagtc cgagccctgg gtcgggcgga 120 gcggagttcg ccgtcttccg gggcttagcc cgagtccgag ccctgggtcg ggcggaacga 180 agttcgccgt cttccggggc ttagcccgag tccgagccct gagtcgggcg gagcggagtt 240 tcctatggcg cctttggcaa ggcctgactg cctgtcagac tcactctgtc gagtggcact 300 gcagtcggag tggcgcaggc ggcgctgtcc ttctgtcaga ccggtcagtg gagcagcgaa 360 gtgacggcgg tcacttcggc tctgccgggg ggcgcgcgtc aggataaagg tgtcaggcca 420 cctttgcgtt aaatgctcct gcgactcggt cggtcggtgc ggcgatttag tcatggttgc 480 ttctcagcga aggcaaggcc tcgggcgagc cggagatatg tccgccgtta aaggggggtc 540 tcgggcgaga cggaaatctc tcggggtcgg ctgcccttgt ccgaggctag gctcgggcga 600 ggcgtgatca agtcgctcgt atggactgat ccctgactta atcgcaccca tcaggcctct 660 gcagctttat gctgatgggg gttaccagct gagaattagg cgtcttgagg gtacccctaa 720 ttatggtccc cgacaataag tatcactgag attattaaat tatatttttg gattgaggga 780 gtagtattta aagaagagcc ttgcatgcaa tttcttttgt ttgaataatg ttttcaactt 840 aaatactttt ttttgcatgt gtaacattta ttctccataa tatttttcca tgtatctaac 900 ttataaatca ttttcataaa ccaatgtcaa agatgaatcc atgtttctta cttggaaacc 960 ccgaacaaac accactagca cgaggcgctc gcgttagcgt cgctcaccga cgacgagaag 1020 aaacttggcg actgtcagtt cgaactctgg aagcagtcct acatggccca tgcgccgctg 1080 tgtacggcaa gctccggcgc agctgccgct tggacgtggt ccagagcggc tgcaccgcgc 1140 tgtccatcgt caaacatggg gacctcatgg tcgtcgccaa tgtcgacgac tctcgggttg 1200 ttctgggcat cgcaacctac gacgacgcca tcacgccgtc tagctcatca tccacctgaa 1260 gcctaacctg ccacgtaagt cgctactgac cgaccatgaa ttcttgtgcg ctggaatgat 1320 gaccattgtt gttgttgtgt gagtgtcctc gaacaagatg tatgtagagg agtagcacat 1380 ccggtggtgc aacgaccagg tgtactacct cgctgatgag cccggggtgc acttcatttg 1440 gcagcccagc caagagtgat cggtactcac catatcgcgc gcgttcgacg actattatat 1500 caaggattgt ggcgtcatct cgacaccgga ggtgagcaga ggtggaccga caacaatgac 1560 cactcgccat cgtcggggta ccttttgtgt cggcctgcct ttaattctct tcgcaaacac 1620 acacttgtat tttttgttta agcaagcgtg tatggtggtg cgtgcctgat gactaacgat 1680 gtacgacgga acttctaccg gcaggtgtgg cacgtgctct ccaataatga gaccatgcaa 1740 atcatgacat atatcgaagt gtgcatgata ctgatagttg caatgtagta gtgaaacaac 1800 taaattaaaa taacaaaatt tatgtatggc taggatcaca aatgaattat gaaatatttt 1860 cttataacaa tataagacac attttgtata taagttatca tggtattata tgttcccgtt 1920 gcaacgcacg ggtactgacc tattgttcat taaggatctt taatagatgt tttttgttat 1980 tttctcgaac ctaaatgttc gtcgaccaaa cttgttttgt atcagtgtat aatattattg 2040 tcactgtttt ttatgtcgcg gactatgttt tttgtacgtc attgaaacta cctataaaat 2100 tgtcactgtg tatcagcgta tgtgtatgca gttggcttga tgtgagacaa taagctgaac 2160 ccacggggtt ttcatggttt tccacccaat tacgagttgg tttgcggtca ataatattat 2220 tggatgggtt gggtttattt tttttcttat attttagggt gggtgtggga taaatatcaa 2280 agttattata cttgggtatg aatgggagtg agttggattt tttgccatta gcaggcctac 2340 atggcggcaa acacaccccc atgtctttat ccccatttca tacaagtcag gtaattaacg 2400 agtatccgct gacatgagac tgttttgagt tttgacgctt tcataccggc ctgatgatga 2460 taccttggca gttggcaaac ggcccatcga tgtccgccat taactgccga tccaatccaa 2520 gtaaccgtag gtatctttct gaccatagtc cacaagtcca aaccagccgt caaaccaacc 2580 gatcatggac atccgatggg catgcttgtt gcgcgatcaa aagtcatcat acaaaaaatg 2640 tgatgaataa aaactggaca aaatttgtgc catcgaatgg agcatacagt cgtggggggc 2700 ctgctacaag accaggcgcc aaccccctgc ctctgggctc tggttggtgg cggtagccgg 2760 gctctcattt aaactaccca tctgcccccg attcttttaa ttacgggcga ccctcgcggc 2820 tccgccccta ttttaagacc ccaccacggc accacctcct ccgcaaacaa cacacgaaca 2880 ccgcaccgca acgtagccgg ctaggggaag agctctttcc ttcagctgtg ccgtgcacaa 2940 cgcacggcac gctggtttgg tttgcatcgc ccccgccaag acgctgccgt gagcaggcag 3000 agcgggtggg aagactggtg ggcgagccgg ccgcgccgag ggatagcagc agcagcagca 3060 ggcgggcgac 3070 <210> 56 <211> 2719 <212> DNA <213> Setaria italica <400> 56 aaagcaaaga ttcatgcctg agctgttggt gacgctatca gctttggctc acctaaaagc 60 agcgccggcc acagctgggg ttcgtttcgt ctactctgat tctcttctga agtctgaagt 120 ccaaaccatg gccaaagtga ctgttgacgc aggcacgtaa atattcaatt gtcacagtgc 180 ggtttggaca ccacgtagac aaacgtttcc tggtcctgag cttttgattg gcccatttca 240 gactagtacg ttaatgcttg cactggcccg aagcggacca cattgctact gtgtgtttat 300 ctgctccgag aaattgggag atagggtggt tagtacagat gagcagctgt tcgctttctc 360 catctatcat tcttcagcac ctgcacttcc aaaaagcttt gaatttggct taggtcatgg 420 aacggctttt gtcaggatct caacgttctc ctttatttgc gagctcaact ccttcctcat 480 agctaactct tcgttcagtc ctccagcatt gccctctgaa gtctgaacca ctccatttct 540 atattgtaca aggaaaagga gtgctatatc agggttgcat ttccaaatct gcaaagcacg 600 cgtacttgaa gccatcaaaa gatgactggc cagcaggagc agcttgccag tgacacagat 660 ttcgtccaaa cggagcacgc agcgcatcgt gctacagcga atcgatcgga tcgagctgag 720 gccaagcatt caaggttttg caggtgcatt gtgatgccga cccgacacac gcgactgctg 780 cagtggctcc atctataaat ctgtcctata taccagcacg cgtgaacaga caaaaaaaaa 840 catcaacaac aagaagagaa gttttactgc tgcttccaga aaccagcctg atcatgagtt 900 tttggctttg agaacgagct gaggatggtt ttccagggca ggtgaacgac atctcgccat 960 ttctcaatcc tctaacctct ccagatgagg ggaaacagtt cagtgctttg cctgtgcatt 1020 gtgcgagaat cgggcgggag cgttcatctt gatccagcac agcagggctt taattgtgca 1080 agcagaaggt accagtacca gcagaggatt gatttgcaca tgtggaagct ctgaagccgt 1140 tgctatcgca tcgttctgac ccggtttggt ggcacgagca aggtagccaa atcaggccag 1200 ccattgcatg tgttgccgta catgcacaag aaagtttaac cgcagtcata tgaagtacta 1260 ctgacccgga tgtactacat gactgcagac tgtgatgctt gctagaggta aaaggacacg 1320 gagggcagga gtcctgccag ttccctcaat gctctgtgct ccgatctctc tagaagggac 1380 ccccttcttt ttatttttga aactatagaa agacaaattt cccttcccct tattagattg 1440 tactaagtac tattttgatt acttgttttt atgaattgtg cagaggctga gaggcctgat 1500 ccaactttgg gtgcggccac aatgtgtggg aaatatgcgc tgtgttcatc tgcacctctc 1560 tgcccccttt atctcaagca aacgtctgta ccttgattat gtacttggta tgattggtgt 1620 gagattcaga tgcaacgatt acttcatatg agccttgctc atcaccgatt ttccctccct 1680 tctctcgtgc ctttttcctt ttcccaaacc actgaagctc aaccagtccg catcagttca 1740 ctactcccgc tagcatggca acggcatgat catgaaggat tccaacaaat ggcctggcag 1800 ctgatggaat tcgactgctc tctctgaaat ttagctactc cttttttcat caattgagtt 1860 ttgacgataa caatttggag tggcgcaatt cacaaccaca accaacactt gtctccctct 1920 ccctggacgg aacagtgcgg ccttgctaat gctaccaaca acacggtgcg cggtcgtacc 1980 cttggccgtt ggcaaatggc cgatcgacat cgccattaac tgccggtcca agctgcgcca 2040 ggcaagccgc gaaagccgta gagtaccttc ccgttcctgc ccaccaacgg cttcctccgc 2100 gcaccgggac gacaacctga tcgacccatc cccaccactt gatcgtcgca caccgatcct 2160 cgcgtgcgct acagttcgtc acgcacccgt acgttcaacc gtcgtctcct cacccctcgc 2220 taaatcctcg ctgttttccc gaaattgctt accaaaaccc cgtgcgcgcg cgtgcccgaa 2280 gctgcagtgc tgcaggcgga tgcgagcgcc ggagcaccgg agccggaggc aggcaggccc 2340 ccgcccccag tggccaaccc ctaactcctc cggttggtgg cggtagccgg gctctcattt 2400 aaactaccca tctgccccgg atttaattac gggcgccccc tttcccggct cgcccactat 2460 attaagaccc caccacggca ccacgtctcc tccgcaaaca aacaccccaa ctcgccaggc 2520 caacgtaccc ccacacaaaa caccgcgacg cagcgccccg gttctcccta gcttcccact 2580 gcgcccaacg cacggcacgc ggttgcagca gcgtccgcga gttccctgcg gagagcagag 2640 gccagaggcg agcggcaggg agggagagtg atcgatcgac tagtgaagtg agccgcgccg 2700 cgcgagggcg gggggcgcc 2719 <210> 57 <211> 2500 <212> DNA <213> Setaria italica <400> 57 aaagcaaaga ttcatgcctg agctgttggt gacgctatca gctttggctc acctaaaagc 60 agcgccggcc acagctgggg ttcgtttcgt ctactctgat tctcttctga agtctgaagt 120 ccaaaccatg gccaaagtga ctgttgacgc aggcacgtaa atattcaatt gtcacagtgc 180 ggtttggaca ccacgtagac aaacgtttcc tggtcctgag cttttgattg gcccatttca 240 gactagtacg ttaatgcttg cactggcccg aagcggacca cattgctact gtgtgtttat 300 ctgctccgag aaattgggag atagggtggt tagtacagat gagcagctgt tcgctttctc 360 catctatcat tcttcagcac ctgcacttcc aaaaagcttt gaatttggct taggtcatgg 420 aacggctttt gtcaggatct caacgttctc ctttatttgc gagctcaact ccttcctcat 480 agctaactct tcgttcagtc ctccagcatt gccctctgaa gtctgaacca ctccatttct 540 atattgtaca aggaaaagga gtgctatatc agggttgcat ttccaaatct gcaaagcacg 600 cgtacttgaa gccatcaaaa gatgactggc cagcaggagc agcttgccag tgacacagat 660 ttcgtccaaa cggagcacgc agcgcatcgt gctacagcga atcgatcgga tcgagctgag 720 gccaagcatt caaggttttg caggtgcatt gtgatgccga cccgacacac gcgactgctg 780 cagtggctcc atctataaat ctgtcctata taccagcacg cgtgaacaga caaaaaaaaa 840 catcaacaac aagaagagaa gttttactgc tgcttccaga aaccagcctg atcatgagtt 900 tttggctttg agaacgagct gaggatggtt ttccagggca ggtgaacgac atctcgccat 960 ttctcaatcc tctaacctct ccagatgagg ggaaacagtt cagtgctttg cctgtgcatt 1020 gtgcgagaat cgggcgggag cgttcatctt gatccagcac agcagggctt taattgtgca 1080 agcagaaggt accagtacca gcagaggatt gatttgcaca tgtggaagct ctgaagccgt 1140 tgctatcgca tcgttctgac ccggtttggt ggcacgagca aggtagccaa atcaggccag 1200 ccattgcatg tgttgccgta catgcacaag aaagtttaac cgcagtcata tgaagtacta 1260 ctgacccgga tgtactacat gactgcagac tgtgatgctt gctagaggta aaaggacacg 1320 gagggcagga gtcctgccag ttccctcaat gctctgtgct ccgatctctc tagaagggac 1380 ccccttcttt ttatttttga aactatagaa agacaaattt cccttcccct tattagattg 1440 tactaagtac tattttgatt acttgttttt atgaattgtg cagaggctga gaggcctgat 1500 ccaactttgg gtgcggccac aatgtgtggg aaatatgcgc tgtgttcatc tgcacctctc 1560 tgcccccttt atctcaagca aacgtctgta ccttgattat gtacttggta tgattggtgt 1620 gagattcaga tgcaacgatt acttcatatg agccttgctc atcaccgatt ttccctccct 1680 tctctcgtgc ctttttcctt ttcccaaacc actgaagctc aaccagtccg catcagttca 1740 ctactcccgc tagcatggca acggcatgat catgaaggat tccaacaaat ggcctggcag 1800 ctgatggaat tcgactgctc tctctgaaat ttagctactc cttttttcat caattgagtt 1860 ttgacgataa caatttggag tggcgcaatt cacaaccaca accaacactt gtctccctct 1920 ccctggacgg aacagtgcgg ccttgctaat gctaccaaca acacggtgcg cggtcgtacc 1980 cttggccgtt ggcaaatggc cgatcgacat cgccattaac tgccggtcca agctgcgcca 2040 ggcaagccgc gaaagccgta gagtaccttc ccgttcctgc ccaccaacgg cttcctccgc 2100 gcaccgggac gacaacctga tcgacccatc cccaccactt gatcgtcgca caccgatcct 2160 cgcgtgcgct acagttcgtc acgcacccgt acgttcaacc gtcgtctcct cacccctcgc 2220 taaatcctcg ctgttttccc gaaattgctt accaaaaccc cgtgcgcgcg cgtgcccgaa 2280 gctgcagtgc tgcaggcgga tgcgagcgcc ggagcaccgg agccggaggc aggcaggccc 2340 ccgcccccag tggccaaccc ctaactcctc cggttggtgg cggtagccgg gctctcattt 2400 aaactaccca tctgccccgg atttaattac gggcgccccc tttcccggct cgcccactat 2460 attaagaccc caccacggca ccacgtctcc tccgcaaaca 2500 <210> 58 <211> 2754 <212> DNA <213> Oryza sativa <400> 58 ggcaggatgt actcacgtac agttagagca ttaacttagg caagcgtttt tctttgcagg 60 taatggcctc tgtcaaacct aatacagatt ggcagtttgg cgcgatggac aggtcatgat 120 caattttaaa taagcagatg atcacattta atgtgtagta tgaagggaac aggtagaata 180 atacttgtaa gtttacaatc ctgtcccacg aagatgtcga tcccctaagt gccgttcgtc 240 gtagtacatg agggacaggt ccaaaacgaa gagacggaat tcgctcgtgc gaaacaaggg 300 caagagagac gtgaaccgaa caggattgcc cttgcacacc tgcggcggat gtatggttgc 360 agctaagctt tattaatgtg agtctctgcc cttcctctgg tcgatcgatg acgagcaaac 420 acagcgcgtt tcgccacgct ttctcccaac ccccaactcc atcccatgtc ggcccatcgt 480 ccattcgtcc tccagaccga tcacatattc agacccgtca acttcagcat ttcacgcggc 540 cactacgaca gctgcttcgg ctgcactttc actcgtatca gcgcgagggg aagaagaaca 600 atccgtggtt ttccgtttct tcagaggcat ggacagatcg tctcacgact cgtacgcgaa 660 gatgcttgat gcttcccgga ggatcctgtc tcgaattttg attggatcac tgcgcactgg 720 acactcacta ctacgttaat gctgctgcca gggctgcagc caaaggacca cactgtcatt 780 ggctccggga caataggagg ccatatattt agccattttt tagggagatt ttcaccaatt 840 gtacagttgc actattgcta cgcagatcac tgcatacgca attaactctt tcttacaagc 900 tcagaagctg tctgcaccta tatagctact ccctcctccg ttctataaaa aaccaaccta 960 ctatcggatg tgacacatcc taatactatg aatctggata tacatcatac taaaatatgt 1020 tatatccggt tctagattcg tttttatgga acggagggag tattctgtca tcagctatca 1080 ctgctctgcc tgaacttgtc tgagctatta ctactgtagt atatgaattt atgaagtgga 1140 gtctcgtaac aaaaatacga gtatactccg tactgtacta cccttatcag gattcaagga 1200 tactaggagt atagcattac cagatcaggc acttagatgt ggccatccaa agagtaatgg 1260 taatagccta ataggagtac catctactat tgatctttca aaaaaaaaag tactagtata 1320 gaagtagtat ccagctagag ctgcttaaca gggtcagaat ttgtccaaac agagcttgta 1380 atgcacactg acgaagtcga tccgatcgaa tcagagtcaa catttaaaga gtactatttc 1440 gaagtaaaac tgttcagttc ttacaagatt acatgtgaat ttgcaagact tggacatgag 1500 tgttcatctc gtagtttaat gatgggtacc caacctgcaa caacagtgct ttaaattgta 1560 caagcagagg gtaccaacag agtaatttgc acatgcagtc ctgaacctga tgccattgcg 1620 aaacacacaa ccctatacgg tgcagatgca agggagccca attcagtccc agccattatc 1680 tgtattgccg tacttccgca gggaaagtta ccctctcatc tgatctacca ttgcatacat 1740 ctcaaaagga agtgagtaaa aaagaaaaca ggaccaatct tcagagataa gtttttgaaa 1800 cctatcttca gagataagtt cactcggcga taggagagga cctgatttgg actcaaatca 1860 ggtgttgacg aatagagtag caagagtttt ttcgacgggt caagacctca agggtcttaa 1920 cagttgaact aactcggtta tgatttggac acttggtgat taactactga taatagtact 1980 acttattgtt attggcttgt tacatcattc ggtgctcaaa aatcagatgc aaatttagat 2040 gggacagaca gcagctacta gcaacataag agaattatgt tcagtgtatc atgcatgact 2100 aaactctgaa cgtactcacc tggatccaga tgcgagcagt acagtacctg tgcacattgc 2160 ttgtgtatta ctccacttaa ttacgaactc tattattttc ctccgataat gcccgcagaa 2220 caaggttgtc actgaaaaat ggtcctctcc agagtccagg agctatagga ggagtatgat 2280 actccttagc aatcatatac tcatatgaca tatccaaatt gacaccgggg ttaagccgtt 2340 aaccgtcact acgagttgca cttgtataaa caaaaaacaa gggagaaaac cttgtgtccc 2400 ccccatgatg cagaaatcta ataagagcag cccaacgctt ccggttggtg gcggtagacc 2460 ggcctcttta aactacccca tccgccccag atttatcaat tactcctcgt catctcgtct 2520 cgtctccgcc accgtgcgcg cgtcccctat attagacccc ccaaccgggc accggacaca 2580 ccatcaccaa cacaccactg caaacccatc cgcctccgca ccgcatcgca cctacaaatt 2640 gtgcacgctg caccgctcaa aaaaaagaag aaactaaagt cgtacgtagg acgcggcgtg 2700 cgagcgttgg tgcggtgcgc ggcggcgcgc ggggaagtag tgagagcatc gatc 2754 <210> 59 <211> 2500 <212> DNA <213> Oryza sativa <400> 59 ggcaggatgt actcacgtac agttagagca ttaacttagg caagcgtttt tctttgcagg 60 taatggcctc tgtcaaacct aatacagatt ggcagtttgg cgcgatggac aggtcatgat 120 caattttaaa taagcagatg atcacattta atgtgtagta tgaagggaac aggtagaata 180 atacttgtaa gtttacaatc ctgtcccacg aagatgtcga tcccctaagt gccgttcgtc 240 gtagtacatg agggacaggt ccaaaacgaa gagacggaat tcgctcgtgc gaaacaaggg 300 caagagagac gtgaaccgaa caggattgcc cttgcacacc tgcggcggat gtatggttgc 360 agctaagctt tattaatgtg agtctctgcc cttcctctgg tcgatcgatg acgagcaaac 420 acagcgcgtt tcgccacgct ttctcccaac ccccaactcc atcccatgtc ggcccatcgt 480 ccattcgtcc tccagaccga tcacatattc agacccgtca acttcagcat ttcacgcggc 540 cactacgaca gctgcttcgg ctgcactttc actcgtatca gcgcgagggg aagaagaaca 600 atccgtggtt ttccgtttct tcagaggcat ggacagatcg tctcacgact cgtacgcgaa 660 gatgcttgat gcttcccgga ggatcctgtc tcgaattttg attggatcac tgcgcactgg 720 acactcacta ctacgttaat gctgctgcca gggctgcagc caaaggacca cactgtcatt 780 ggctccggga caataggagg ccatatattt agccattttt tagggagatt ttcaccaatt 840 gtacagttgc actattgcta cgcagatcac tgcatacgca attaactctt tcttacaagc 900 tcagaagctg tctgcaccta tatagctact ccctcctccg ttctataaaa aaccaaccta 960 ctatcggatg tgacacatcc taatactatg aatctggata tacatcatac taaaatatgt 1020 tatatccggt tctagattcg tttttatgga acggagggag tattctgtca tcagctatca 1080 ctgctctgcc tgaacttgtc tgagctatta ctactgtagt atatgaattt atgaagtgga 1140 gtctcgtaac aaaaatacga gtatactccg tactgtacta cccttatcag gattcaagga 1200 tactaggagt atagcattac cagatcaggc acttagatgt ggccatccaa agagtaatgg 1260 taatagccta ataggagtac catctactat tgatctttca aaaaaaaaag tactagtata 1320 gaagtagtat ccagctagag ctgcttaaca gggtcagaat ttgtccaaac agagcttgta 1380 atgcacactg acgaagtcga tccgatcgaa tcagagtcaa catttaaaga gtactatttc 1440 gaagtaaaac tgttcagttc ttacaagatt acatgtgaat ttgcaagact tggacatgag 1500 tgttcatctc gtagtttaat gatgggtacc caacctgcaa caacagtgct ttaaattgta 1560 caagcagagg gtaccaacag agtaatttgc acatgcagtc ctgaacctga tgccattgcg 1620 aaacacacaa ccctatacgg tgcagatgca agggagccca attcagtccc agccattatc 1680 tgtattgccg tacttccgca gggaaagtta ccctctcatc tgatctacca ttgcatacat 1740 ctcaaaagga agtgagtaaa aaagaaaaca ggaccaatct tcagagataa gtttttgaaa 1800 cctatcttca gagataagtt cactcggcga taggagagga cctgatttgg actcaaatca 1860 ggtgttgacg aatagagtag caagagtttt ttcgacgggt caagacctca agggtcttaa 1920 cagttgaact aactcggtta tgatttggac acttggtgat taactactga taatagtact 1980 acttattgtt attggcttgt tacatcattc ggtgctcaaa aatcagatgc aaatttagat 2040 gggacagaca gcagctacta gcaacataag agaattatgt tcagtgtatc atgcatgact 2100 aaactctgaa cgtactcacc tggatccaga tgcgagcagt acagtacctg tgcacattgc 2160 ttgtgtatta ctccacttaa ttacgaactc tattattttc ctccgataat gcccgcagaa 2220 caaggttgtc actgaaaaat ggtcctctcc agagtccagg agctatagga ggagtatgat 2280 actccttagc aatcatatac tcatatgaca tatccaaatt gacaccgggg ttaagccgtt 2340 aaccgtcact acgagttgca cttgtataaa caaaaaacaa gggagaaaac cttgtgtccc 2400 ccccatgatg cagaaatcta ataagagcag cccaacgctt ccggttggtg gcggtagacc 2460 ggcctcttta aactacccca tccgccccag atttatcaat 2500 <210> 60 <211> 879 <212> DNA <213> Arabidopsis thaliana <400> 60 atggagcctc ctcagcacca acatcaccac catcaggccg atcaagagtc cgggaacaac 60 aacaacaaca agtccggttc cggagggtac acttgcagac agacctccac cagatggaca 120 cctaccactg agcagatcaa gatcctgaag gagttgtact acaacaatgc catccgctcc 180 cctactgctg atcagattca gaagatcacc gctcgcctgc gtcaattcgg taagatcgag 240 ggtaagaacg tcttctactg gttccagaac cacaaggctc gtgagaggca gaagaagcgt 300 ttcaacggga ctaacatgac cactccttcc tcatcaccca attctgttat gatggccgcc 360 aacgaccact accatccttt gcttcatcac caccacggag ttcctatgca aagacccgca 420 aactccgtca acgtgaagtt gaaccaggac catcacctct accaccacaa caagccctac 480 ccctctttca acaacgggaa tctcaaccat gcctcctctg gaaccgaatg tggagttgtc 540 aacgcctcca acggctacat gtcttcccac gtgtacggct ctatggagca agattgctcc 600 atgaactaca acaacgttgg aggcggatgg gcaaacatgg atcaccacta ctcctccgct 660 ccctacaact tcttcgatag ggctaaacct ctcttcggct tggagggaca ccaagaagaa 720 gaggaatgcg gaggtgatgc ttacttggaa cacagaagga ctctccctct ctttccaatg 780 cacggtgagg accacatcaa cggaggatct ggcgctatct ggaagtatgg acagtccgag 840 gtcagaccat gcgcttccct ggaattgaga ctgaactag 879 <210> 61 <211> 292 <212> PRT <213> Arabidopsis thaliana <400> 61 Met Glu Pro Pro Gln His Gln His His His His Gln Ala Asp Gln Glu 1 5 10 15 Ser Gly Asn Asn Asn Asn Asn Lys Ser Gly Ser Gly Gly Tyr Thr Cys 20 25 30 Arg Gln Thr Ser Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile Lys Ile 35 40 45 Leu Lys Glu Leu Tyr Tyr Asn Asn Ala Ile Arg Ser Pro Thr Ala Asp 50 55 60 Gln Ile Gln Lys Ile Thr Ala Arg Leu Arg Gln Phe Gly Lys Ile Glu 65 70 75 80 Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg 85 90 95 Gln Lys Lys Arg Phe Asn Gly Thr Asn Met Thr Thr Pro Ser Ser Ser 100 105 110 Pro Asn Ser Val Met Met Ala Ala Asn Asp His Tyr His Pro Leu Leu 115 120 125 His His His His Gly Val Pro Met Gln Arg Pro Ala Asn Ser Val Asn 130 135 140 Val Lys Leu Asn Gln Asp His His Leu Tyr His His Asn Lys Pro Tyr 145 150 155 160 Pro Ser Phe Asn Asn Gly Asn Leu Asn His Ala Ser Ser Gly Thr Glu 165 170 175 Cys Gly Val Val Asn Ala Ser Asn Gly Tyr Met Ser Ser His Val Tyr 180 185 190 Gly Ser Met Glu Gln Asp Cys Ser Met Asn Tyr Asn Asn Val Gly Gly 195 200 205 Gly Trp Ala Asn Met Asp His His Tyr Ser Ser Ala Pro Tyr Asn Phe 210 215 220 Phe Asp Arg Ala Lys Pro Leu Phe Gly Leu Glu Gly His Gln Glu Glu 225 230 235 240 Glu Glu Cys Gly Gly Asp Ala Tyr Leu Glu His Arg Arg Thr Leu Pro 245 250 255 Leu Phe Pro Met His Gly Glu Asp His Ile Asn Gly Gly Ser Gly Ala 260 265 270 Ile Trp Lys Tyr Gly Gln Ser Glu Val Arg Pro Cys Ala Ser Leu Glu 275 280 285 Leu Arg Leu Asn 290 <210> 62 <211> 876 <212> DNA <213> Lotus japonicus <400> 62 atggaacaac cacctcaaca acaacaacag aatgaggatg gtggtgggag tagtggtgga 60 aaaggggggt ttctgagcag gcaaagcagt acacggtgga ctcccaccac agaccagata 120 agaatattga aggagcttta ctacaacaat ggaataagat ccccgagtgc agagcagatt 180 cagaggatct ctgctcggct gagacagtac ggcaagattg aaggcaagaa tgtcttttac 240 tggttccaga accacaaagc tagagagagg cagaagaaaa ggttcacttc tgaaactcat 300 cttcttccca atcccataca acaacaacaa agaaatggaa ctaatgctgc ttggaagcaa 360 cctgaagaac aacccattat taaccacacc aagtattcta gtaacaacat ttcagctcct 420 gcagggatca tcacttctgc tccatcttct tctgcagaga ttgtttctgt tggccagata 480 ggaaactttg gttatggatc tttgcctatg gaaaagagtt ttagggactg ctcaatatct 540 gctggaggca acacaggcta tgctggaagt gctataaacc acaacctggg atggatgggt 600 gtggatccat attcctcagc ctacaccaat ttctttgaca aaataaggcc aactgaagaa 660 gaaaccatgg aagaagaagg acaagagaat ggttcaccag agattgaaac ccttccttta 720 ttcccgatgc atggtgagga cattcatagt ggctatttca acctcaagtc taattcttct 780 cactatgctg gtggctggta ccagactgaa gaagggtact tcaacaatgg ttctcgtgct 840 tccttggagc tcagcctcaa atatcctgat tttgct 876 <210> 63 <211> 292 <212> PRT <213> Lotus japonicus <400> 63 Met Glu Gln Pro Pro Gln Gln Gln Gln Gln Asn Glu Asp Gly Gly Gly 1 5 10 15 Ser Ser Gly Gly Lys Gly Gly Phe Leu Ser Arg Gln Ser Ser Thr Arg 20 25 30 Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Glu Leu Tyr Tyr 35 40 45 Asn Asn Gly Ile Arg Ser Pro Ser Ala Glu Gln Ile Gln Arg Ile Ser 50 55 60 Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr 65 70 75 80 Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe Thr 85 90 95 Ser Glu Thr His Leu Leu Pro Asn Pro Ile Gln Gln Gln Gln Arg Asn 100 105 110 Gly Thr Asn Ala Ala Trp Lys Gln Pro Glu Glu Gln Pro Ile Ile Asn 115 120 125 His Thr Lys Tyr Ser Ser Asn Asn Ile Ser Ala Pro Ala Gly Ile Ile 130 135 140 Thr Ser Ala Pro Ser Ser Ser Ala Glu Ile Val Ser Val Gly Gln Ile 145 150 155 160 Gly Asn Phe Gly Tyr Gly Ser Leu Pro Met Glu Lys Ser Phe Arg Asp 165 170 175 Cys Ser Ile Ser Ala Gly Gly Asn Thr Gly Tyr Ala Gly Ser Ala Ile 180 185 190 Asn His Asn Leu Gly Trp Met Gly Val Asp Pro Tyr Ser Ser Ala Tyr 195 200 205 Thr Asn Phe Phe Asp Lys Ile Arg Pro Thr Glu Glu Glu Thr Met Glu 210 215 220 Glu Glu Gly Gln Glu Asn Gly Ser Pro Glu Ile Glu Thr Leu Pro Leu 225 230 235 240 Phe Pro Met His Gly Glu Asp Ile His Ser Gly Tyr Phe Asn Leu Lys 245 250 255 Ser Asn Ser Ser His Tyr Ala Gly Gly Trp Tyr Gln Thr Glu Glu Gly 260 265 270 Tyr Phe Asn Asn Gly Ser Arg Ala Ser Leu Glu Leu Ser Leu Lys Tyr 275 280 285 Pro Asp Phe Ala 290 <210> 64 <211> 888 <212> DNA <213> Glycine max <400> 64 atggaacctc aacaacaaca acaacaagca caagggagcc aacaacaaca acaaaacgag 60 gatggtggca gtggaaaagg ggggtttctg agcaggcaaa gtagtacacg gtggactcca 120 acaaacgacc agataagaat attgaaggaa ctttactaca acaatggaat tagatccccg 180 agtgcagagc agattcagag gatctctgct aggctgaggc agtacggtaa gattgaaggc 240 aagaatgtct tttattggtt ccagaaccac aaagctcgag aaaggcagaa gaaaaggttc 300 acttctgatc ataatcataa taatgtcccc atgcaaagac ccccaactaa tccttctgct 360 gcttggaaac ctgatctagc tgatcccatt cacaccacca agtattgtaa catctcttct 420 actgcaggga tctcttcggc atcatcttct gttgagatgg ttactgtggg acagatgggg 480 aattatgggt atggttctgt gcctatggag aaaagtttta gggactgctc gatatcagct 540 gggggtagca gtggccatgt tggattaata aaccacaact tggggtgggt tggtgtggac 600 ccatataatt cctcaaccta tgccaacttc tttgacaaaa taaggccaag tgatcaagaa 660 acccttgaag aagaagcaga gaacattggt gctactaaga ttgaaaccct ccctttattc 720 cctatgcacg gtgaggacat ccacggctat tgcaacctca agtctaattc gtataactat 780 gatggaaacg gctggtatca tactgaagaa gggttcaaga atgcttctcg tgcttccttg 840 gagctcagtc tcaactccta cactcgcagg tctccagatt atgcttaa 888 <210> 65 <211> 295 <212> PRT <213> Glycine max <400> 65 Met Glu Pro Gln Gln Gln Gln Gln Gln Ala Gln Gly Ser Gln Gln Gln 1 5 10 15 Gln Gln Asn Glu Asp Gly Gly Ser Gly Lys Gly Gly Phe Leu Ser Arg 20 25 30 Gln Ser Ser Thr Arg Trp Thr Pro Thr Asn Asp Gln Ile Arg Ile Leu 35 40 45 Lys Glu Leu Tyr Tyr Asn Asn Gly Ile Arg Ser Pro Ser Ala Glu Gln 50 55 60 Ile Gln Arg Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly 65 70 75 80 Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln 85 90 95 Lys Lys Arg Phe Thr Ser Asp His Asn His Asn Asn Val Pro Met Gln 100 105 110 Arg Pro Pro Thr Asn Pro Ser Ala Ala Trp Lys Pro Asp Leu Ala Asp 115 120 125 Pro Ile His Thr Thr Lys Tyr Cys Asn Ile Ser Ser Thr Ala Gly Ile 130 135 140 Ser Ser Ala Ser Ser Ser Val Glu Met Val Thr Val Gly Gln Met Gly 145 150 155 160 Asn Tyr Gly Tyr Gly Ser Val Pro Met Glu Lys Ser Phe Arg Asp Cys 165 170 175 Ser Ile Ser Ala Gly Gly Ser Ser Gly His Val Gly Leu Ile Asn His 180 185 190 Asn Leu Gly Trp Val Gly Val Asp Pro Tyr Asn Ser Ser Thr Tyr Ala 195 200 205 Asn Phe Phe Asp Lys Ile Arg Pro Ser Asp Gln Glu Thr Leu Glu Glu 210 215 220 Glu Ala Glu Asn Ile Gly Ala Thr Lys Ile Glu Thr Leu Pro Leu Phe 225 230 235 240 Pro Met His Gly Glu Asp Ile His Gly Tyr Cys Asn Leu Lys Ser Asn 245 250 255 Ser Tyr Asn Tyr Asp Gly Asn Gly Trp Tyr His Thr Glu Glu Gly Phe 260 265 270 Lys Asn Ala Ser Arg Ala Ser Leu Glu Leu Ser Leu Asn Ser Tyr Thr 275 280 285 Arg Arg Ser Pro Asp Tyr Ala 290 295 <210> 66 <211> 897 <212> DNA <213> Camelina sativa <400> 66 atggagccac cgcagcatca gcatcatcac catcaagccg accaagaaag cggcaataac 60 aacaagtccg gttctggtgg ttacacttgt cgccaaacca gcacgaggtg gacaccaacg 120 acggagcaga tcaaaatgct caaagagctt tattacaata gtggaatccg gtcaccaaca 180 gcagatcaga tccagaagat cactgctagg ctgcgacagt tcggaaagat cgagggcaag 240 aacgtctttt actggttcca gaaccacaag gctcgtgagc gtcagaagaa gagattcaac 300 ggcacaacca tgaccacacc gtcttcatct cccaactcgg ttatgatggc taatgatcat 360 tatcatcaac atctccctct acttcaccat catcatggcg ttcccatgca gagacctgcg 420 aactccgtca acgttaaact taaccaagag catcatctct atcatcagaa caagtcatat 480 cccagcttca ataacgggaa tttaaatcat gcaagctcag gtactgaatg tggtgctgtt 540 aatgcttcta atggctactc aagtagccat atctatggat ctatggaaca agactgttct 600 atgaattaca acaacgtagg tggaggatgg acggcaaaca tggatcataa tcatcattac 660 tcatcagcac cttacaactt cttcgataga ccaaaacctc cgtttggtct agacggtcat 720 caggaagaag aagaatgtgg tggcgatgct tatctggaac atcgacgtac gcttcctctc 780 ttccctatgc atggtgaaga tcacataaat ggtgccagct ggaagtatgg ccaactggac 840 ggtcatgatt gtcatggtag aggcccttgc gcttctcttg agcttcgttt gaactag 897 <210> 67 <211> 298 <212> PRT <213> Camelina sativa <400> 67 Met Glu Pro Pro Gln His Gln His His His His Gln Ala Asp Gln Glu 1 5 10 15 Ser Gly Asn Asn Asn Lys Ser Gly Ser Gly Gly Tyr Thr Cys Arg Gln 20 25 30 Thr Ser Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile Lys Met Leu Lys 35 40 45 Glu Leu Tyr Tyr Asn Ser Gly Ile Arg Ser Pro Thr Ala Asp Gln Ile 50 55 60 Gln Lys Ile Thr Ala Arg Leu Arg Gln Phe Gly Lys Ile Glu Gly Lys 65 70 75 80 Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys 85 90 95 Lys Arg Phe Asn Gly Thr Thr Met Thr Thr Pro Ser Ser Ser Pro Asn 100 105 110 Ser Val Met Met Ala Asn Asp His Tyr His Gln His Leu Pro Leu Leu 115 120 125 His His His His Gly Val Pro Met Gln Arg Pro Ala Asn Ser Val Asn 130 135 140 Val Lys Leu Asn Gln Glu His His Leu Tyr His Gln Asn Lys Ser Tyr 145 150 155 160 Pro Ser Phe Asn Asn Gly Asn Leu Asn His Ala Ser Ser Gly Thr Glu 165 170 175 Cys Gly Ala Val Asn Ala Ser Asn Gly Tyr Ser Ser Ser His Ile Tyr 180 185 190 Gly Ser Met Glu Gln Asp Cys Ser Met Asn Tyr Asn Asn Val Gly Gly 195 200 205 Gly Trp Thr Ala Asn Met Asp His Asn His His Tyr Ser Ser Ala Pro 210 215 220 Tyr Asn Phe Phe Asp Arg Pro Lys Pro Pro Phe Gly Leu Asp Gly His 225 230 235 240 Gln Glu Glu Glu Glu Cys Gly Gly Asp Ala Tyr Leu Glu His Arg Arg 245 250 255 Thr Leu Pro Leu Phe Pro Met His Gly Glu Asp His Ile Asn Gly Ala 260 265 270 Ser Trp Lys Tyr Gly Gln Leu Asp Gly His Asp Cys His Gly Arg Gly 275 280 285 Pro Cys Ala Ser Leu Glu Leu Arg Leu Asn 290 295 <210> 68 <211> 897 <212> DNA <213> Capsella rubella <400> 68 atggagccac cacagcatca gcatcatcat catcaagccg accaagaaag cggcaacaac 60 aacaacaagt ccggctctgg tggctacacg tgtcgccaaa ccagcactag gtggacacca 120 acgacggagc agatcagaat cctcaaggat ctttactaca acagtgcgat ccggtcacca 180 acagccgatc agatccagaa gatcaccgcg aggctgagac agtacgggaa gatcgaaggc 240 aagaacgtct tttactggtt ccagaaccat aaggctcgtg agcgtcagaa gaagagattc 300 aacggcacaa ccatgaccac tccatcttca tctcccaact cggttatggt ggctaatgat 360 cactatcatc ctctacttca ccatcatcat ggtgttccca tgcaaaggcc tgctaactcc 420 gtcaacgtca aacttaacca agaccatcat ctctatcatc agaacaagtc atatcccacc 480 ttcaacaatg ggaatttaaa tcatggaagc tcaggtactg aatgtggtgt tgttaatgct 540 tctaatggct acatgagtag ccatgtctat ggatctatgg aacaagactg ttcaatgaac 600 tacaacaacg taggtactgg aggatacgga tcaaacatgg atcatagtca tcattactca 660 tctgcaccat acaacttctt tgatagatca aagcctctct ttggtctaga aggtcatcaa 720 gaagatgaag aagaatatgg tataggcgat gcttatctgg aacatcgacg tacgcttcct 780 ctcttcccta tgcatggtga agatcacatg aacggtagtg gtgctagctg gaagtttgat 840 ggccgtgatt gccatggtag aggcccttgc gcttctcttg agcttcgtct gaactag 897 <210> 69 <211> 298 <212> PRT <213> Capsella rubella <400> 69 Met Glu Pro Pro Gln His Gln His His His His Gln Ala Asp Gln Glu 1 5 10 15 Ser Gly Asn Asn Asn Asn Lys Ser Gly Ser Gly Gly Tyr Thr Cys Arg 20 25 30 Gln Thr Ser Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile Arg Ile Leu 35 40 45 Lys Asp Leu Tyr Tyr Asn Ser Ala Ile Arg Ser Pro Thr Ala Asp Gln 50 55 60 Ile Gln Lys Ile Thr Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly 65 70 75 80 Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln 85 90 95 Lys Lys Arg Phe Asn Gly Thr Thr Met Thr Thr Pro Ser Ser Ser Pro 100 105 110 Asn Ser Val Met Val Ala Asn Asp His Tyr His Pro Leu Leu His His 115 120 125 His His Gly Val Pro Met Gln Arg Pro Ala Asn Ser Val Asn Val Lys 130 135 140 Leu Asn Gln Asp His His Leu Tyr His Gln Asn Lys Ser Tyr Pro Thr 145 150 155 160 Phe Asn Asn Gly Asn Leu Asn His Gly Ser Ser Gly Thr Glu Cys Gly 165 170 175 Val Val Asn Ala Ser Asn Gly Tyr Met Ser Ser His Val Tyr Gly Ser 180 185 190 Met Glu Gln Asp Cys Ser Met Asn Tyr Asn Asn Val Gly Thr Gly Gly 195 200 205 Tyr Gly Ser Asn Met Asp His Ser His His Tyr Ser Ser Ala Pro Tyr 210 215 220 Asn Phe Phe Asp Arg Ser Lys Pro Leu Phe Gly Leu Glu Gly His Gln 225 230 235 240 Glu Asp Glu Glu Glu Tyr Gly Ile Gly Asp Ala Tyr Leu Glu His Arg 245 250 255 Arg Thr Leu Pro Leu Phe Pro Met His Gly Glu Asp His Met Asn Gly 260 265 270 Ser Gly Ala Ser Trp Lys Phe Asp Gly Arg Asp Cys His Gly Arg Gly 275 280 285 Pro Cys Ala Ser Leu Glu Leu Arg Leu Asn 290 295 <210> 70 <211> 588 <212> DNA <213> Arabis alpina <400> 70 atggctactc atgtagccat tagaagcatt aacagcacca cattcagaac ctgaacttgc 60 atgatttaaa ttcccgttat tgaagctggg atatgatttg ttctgatgaa gcagatggtg 120 ttcttggtca tgaagcttaa cgttgagaga agcaggtctc tgcatggaaa caccatgatg 180 atgttgaaga agaggatgat gatgatggtt atgatgataa cgatcactgg ccatcataac 240 tgagttgggc gatgaagacg ttggtgtggt ggtcatggtt gtgccattga atctcttctt 300 ctgacgctca cgagccttat ggttctgaaa ccagtaaaag acgtttttgc cctcgatctt 360 accatattgt ctcagccttg cagagatctt ctggatctca tcagcagttg gtgaccggat 420 tccactattg tagtaaagat ctttgaggat tctgatttgc tccgtggttg gagtccatct 480 cgtgctggtt tgacgacaag tgtaaccacc agaaccggac ttgttgttgt tgttgccgct 540 ttcttggtcg gcttgatgat gatgatgatg atgttgcggt ggctccat 588 <210> 71 <211> 304 <212> PRT <213> Arabis alpina <400> 71 Met Glu Pro Pro Gln His His His His His His Gln Ala Asp Gln Glu 1 5 10 15 Ser Gly Asn Asn Asn Asn Lys Ser Gly Ser Gly Gly Tyr Thr Cys Arg 20 25 30 Gln Thr Ser Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile Arg Ile Leu 35 40 45 Lys Asp Leu Tyr Tyr Asn Ser Gly Ile Arg Ser Pro Thr Ala Asp Glu 50 55 60 Ile Gln Lys Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly 65 70 75 80 Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln 85 90 95 Lys Lys Arg Phe Asn Gly Thr Thr Met Thr Thr Thr Pro Thr Ser Ser 100 105 110 Ser Pro Asn Ser Val Met Met Ala Ser Asp Arg Tyr His His Asn His 115 120 125 His His His Pro Leu Leu Gln His His His Gly Val Ser Met Gln Arg 130 135 140 Pro Ala Ser Leu Asn Val Lys Leu His Asp Gln Glu His His Leu Leu 145 150 155 160 His Gln Asn Lys Ser Tyr Pro Ser Phe Asn Asn Gly Asn Leu Asn His 165 170 175 Ala Ser Ser Gly Ser Glu Cys Gly Ala Val Asn Ala Ser Asn Gly Tyr 180 185 190 Met Ser Ser His Val Tyr Gly Ser Met Glu Gln Asp Tyr Ser Met Ser 195 200 205 Asn Asn Asn Ile Gly Gly Gly Gly Trp Ser Asn Met Asp His Asn His 210 215 220 His Tyr Ser Ser Pro Ala Tyr Asn Phe Phe Asp Arg Pro Lys Pro Leu 225 230 235 240 Phe Gly Leu Glu Gly His Gln Glu Glu Asp Gln Tyr Gly Gly Asp Ala 245 250 255 Tyr Leu Glu His Arg Arg Thr Leu Pro Leu Phe Pro Met His Gly Glu 260 265 270 Asp His Ile Asn Gly Gly Gly Gly Ala Ile Trp Lys Tyr Arg Gln Leu 275 280 285 Asp Gly Leu Glu Leu Arg Leu Asn Ser Tyr Ala Gly Val Ala Pro Glu 290 295 300 <210> 72 <211> 873 <212> DNA <213> Raphanus sativus <400> 72 atggagccac cgcaacatta ccatcaagcc gatcaagaaa gcggcaacaa caacaagtcc 60 gtctctggtg gttacacgtg tcgtcaaacg agcacaagat ggacaccaac aaccgatcaa 120 atcagaatac tcaaagatct ttactacaac aatggagtcc ggtcaccaac agccgaccag 180 atccagaaga tctctgcaac gctgagacag tacggaaaaa tcgagggaaa aaatgtcttt 240 tactggtttc aaaaccataa ggctcgtgag cgacagaaga agagattcaa cagcacaacc 300 atggccacac caacgtcttc atcgcccaac tcggttatga tgatggctag tgatcaccat 360 catcatcatg gtgttaccat ccagagacct gctttggtca acgttaagct cgaccaagaa 420 aatcatatgt ttcaccagaa cagatcatat cccagctcca ataacgggag tataaatcat 480 gcaagttcag gcacggaata tggtgttttc ggcgcttcta atggctacat tagtaaccat 540 atctatggat ctatggaaca agactgttca atgagctaca acagcgtagg tggaggatgg 600 acaaacatgg atcataatca tcattactca actccagctt acaacttctt tgatagacca 660 tcgcctctgt ctggactaga aggtcatcaa gaagaaggac aatatggtgg cgattcttat 720 ctggaacatc gacgtacact tcctctcttc cctttgcacg gtgaggatca catcaacggt 780 ggtggtggtt ccatcttgaa gtacggacaa tcagacggtt gtgatcgtta tggtagaggc 840 ccttgtgctt ctcttaagct atgtctgaac tga 873 <210> 73 <211> 290 <212> PRT <213> Raphanus sativus <400> 73 Met Glu Pro Pro Gln His Tyr His Gln Ala Asp Gln Glu Ser Gly Asn 1 5 10 15 Asn Asn Lys Ser Val Ser Gly Gly Tyr Thr Cys Arg Gln Thr Ser Thr 20 25 30 Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp Leu Tyr 35 40 45 Tyr Asn Asn Gly Val Arg Ser Pro Thr Ala Asp Gln Ile Gln Lys Ile 50 55 60 Ser Ala Thr Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe 65 70 75 80 Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe 85 90 95 Asn Ser Thr Thr Met Ala Thr Pro Thr Ser Ser Ser Pro Asn Ser Val 100 105 110 Met Met Met Ala Ser Asp His His His His His Gly Val Thr Ile Gln 115 120 125 Arg Pro Ala Leu Val Asn Val Lys Leu Asp Gln Glu Asn His Met Phe 130 135 140 His Gln Asn Arg Ser Tyr Pro Ser Ser Asn Asn Gly Ser Ile Asn His 145 150 155 160 Ala Ser Ser Gly Thr Glu Tyr Gly Val Phe Gly Ala Ser Asn Gly Tyr 165 170 175 Ile Ser Asn His Ile Tyr Gly Ser Met Glu Gln Asp Cys Ser Met Ser 180 185 190 Tyr Asn Ser Val Gly Gly Gly Trp Thr Asn Met Asp His Asn His His 195 200 205 Tyr Ser Thr Pro Ala Tyr Asn Phe Phe Asp Arg Pro Ser Pro Leu Ser 210 215 220 Gly Leu Glu Gly His Gln Glu Glu Gly Gln Tyr Gly Gly Asp Ser Tyr 225 230 235 240 Leu Glu His Arg Arg Thr Leu Pro Leu Phe Pro Leu His Gly Glu Asp 245 250 255 His Ile Asn Gly Gly Gly Gly Ser Ile Leu Lys Tyr Gly Gln Ser Asp 260 265 270 Gly Cys Asp Arg Tyr Gly Arg Gly Pro Cys Ala Ser Leu Lys Leu Cys 275 280 285 Leu Asn 290 <210> 74 <211> 885 <212> DNA <213> Brassica napus <400> 74 atggagccag cgcaacatca ccatcaagcc gaccaagaaa gcggcaacaa caacaagtcc 60 gtctctggtg gttacacgtg tcgtcaaacg agtacaagat ggacaccaac gaccgatcaa 120 atcagaatac tcaaagatct ttactacaac aacggagtcc ggtcaccaac agccgaccag 180 atccagaaga tctctgcaag gctgagacag tacggaaaga tcgagggaaa aaatgtcttt 240 tactggtttc aaaaccataa ggctcgtgag cgacagaaga agagattcaa cagcacaacc 300 atgaccatac caacgtcttc atcgcccaac tcggttatga tggctagtga tcactatcac 360 cataaccatc atcatcatgg cgttaccatc cagagacctg ctttggtcaa cgttaagctc 420 gaccaagaaa atcatatgtt tcatcagaac agatcatatc ccagcttcaa taacgggaat 480 acaaatcatg caagttcagg cacggaatat ggtgttttca gtgcttctaa tggctacatt 540 agtagccata tctatgaacc tatggaacaa gactgttcaa tgagctacaa caacgtaggt 600 ggaggatgga caaacataga tcataatcac cattactcaa ctccagccta caacttctta 660 gatagaccaa tgcctctgtc tggactagaa ggtcatcatc aagaagaagg agaatatggt 720 ggcgatgctt atctggaaca tagacgcaca cttcctctct tccctttgca cggtgaggat 780 cacatcaacg gcggtggtgg ttccatctgg aagtacagac aatcggacgg ttgtgatcgt 840 tatggtagag gcccttgtgc ttctcttaag ctgtgtctga actga 885 <210> 75 <211> 294 <212> PRT <213> Brassica napus <400> 75 Met Glu Pro Ala Gln His His His Gln Ala Asp Gln Glu Ser Gly Asn 1 5 10 15 Asn Asn Lys Ser Val Ser Gly Gly Tyr Thr Cys Arg Gln Thr Ser Thr 20 25 30 Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp Leu Tyr 35 40 45 Tyr Asn Asn Gly Val Arg Ser Pro Thr Ala Asp Gln Ile Gln Lys Ile 50 55 60 Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe 65 70 75 80 Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe 85 90 95 Asn Ser Thr Thr Met Thr Ile Pro Thr Ser Ser Ser Pro Asn Ser Val 100 105 110 Met Met Ala Ser Asp His Tyr His His Asn His His His His Gly Val 115 120 125 Thr Ile Gln Arg Pro Ala Leu Val Asn Val Lys Leu Asp Gln Glu Asn 130 135 140 His Met Phe His Gln Asn Arg Ser Tyr Pro Ser Phe Asn Asn Gly Asn 145 150 155 160 Thr Asn His Ala Ser Ser Gly Thr Glu Tyr Gly Val Phe Ser Ala Ser 165 170 175 Asn Gly Tyr Ile Ser Ser His Ile Tyr Glu Pro Met Glu Gln Asp Cys 180 185 190 Ser Met Ser Tyr Asn Asn Val Gly Gly Gly Trp Thr Asn Ile Asp His 195 200 205 Asn His His Tyr Ser Thr Pro Ala Tyr Asn Phe Leu Asp Arg Pro Met 210 215 220 Pro Leu Ser Gly Leu Glu Gly His His Gln Glu Glu Gly Glu Tyr Gly 225 230 235 240 Gly Asp Ala Tyr Leu Glu His Arg Arg Thr Leu Pro Leu Phe Pro Leu 245 250 255 His Gly Glu Asp His Ile Asn Gly Gly Gly Gly Ser Ile Trp Lys Tyr 260 265 270 Arg Gln Ser Asp Gly Cys Asp Arg Tyr Gly Arg Gly Pro Cys Ala Ser 275 280 285 Leu Lys Leu Cys Leu Asn 290 <210> 76 <211> 885 <212> DNA <213> Brassica oleracea <400> 76 atggagccag cgcaacatca ccatcaagcc gaccaagaaa gcggcaacaa caacaagtcc 60 gtctctggtg gttacacgtg tcgtcaaacg agtacaagat ggacaccaac gaccgatcaa 120 atcagaatac tcaaagatct ttactacaac aacggagtcc ggtcaccaac agccgaccag 180 atccagaaga tctctgcaag gctgagacag tacggaaaga tcgagggaaa aaatgtcttt 240 tactggtttc aaaaccataa ggctcgtgag cgacagaaga agagattcaa cagcacaacc 300 atgaccatac caacgtcttc atcgcccaac tcggttatga tggctagtga tcactatcac 360 cataaccatc atcatcatgg cgttaccatc cagagacctg ctttggtcaa cgttaagctc 420 gaccaagaaa atcatatgtt tcatcagaac agatcatatc ccagcttcaa taacgggaat 480 ataaatcatg caagttcagg cacggaatat ggtgttttca gtgcttctaa tggctacatt 540 agtagccata tctatggacc tatggaacaa gactgttcaa tgagctacaa caacgtaggt 600 ggaggatgga caaatataga tcataatcac cattactcaa ctccagccta caacttctta 660 gatagaccaa tgcctctgtc tggactagaa ggtcatcatc aagaagaagg agaatatggt 720 ggcgatgctt atctggaaca tagacgcaca cttcctctct tccctttgca cggtgaggat 780 cacatcaacg gcggtggtgg ttccatctgg aagtacagac aatcggacgg ttgtgatcgt 840 tatggtagag gcccttgtgc ttctcttaag ctgtgtctga actga 885 <210> 77 <211> 294 <212> PRT <213> Brassica oleracea <400> 77 Met Glu Pro Ala Gln His His His Gln Ala Asp Gln Glu Ser Gly Asn 1 5 10 15 Asn Asn Lys Ser Val Ser Gly Gly Tyr Thr Cys Arg Gln Thr Ser Thr 20 25 30 Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp Leu Tyr 35 40 45 Tyr Asn Asn Gly Val Arg Ser Pro Thr Ala Asp Gln Ile Gln Lys Ile 50 55 60 Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe 65 70 75 80 Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe 85 90 95 Asn Ser Thr Thr Met Thr Ile Pro Thr Ser Ser Ser Pro Asn Ser Val 100 105 110 Met Met Ala Ser Asp His Tyr His His Asn His His His His Gly Val 115 120 125 Thr Ile Gln Arg Pro Ala Leu Val Asn Val Lys Leu Asp Gln Glu Asn 130 135 140 His Met Phe His Gln Asn Arg Ser Tyr Pro Ser Phe Asn Asn Gly Asn 145 150 155 160 Ile Asn His Ala Ser Ser Gly Thr Glu Tyr Gly Val Phe Ser Ala Ser 165 170 175 Asn Gly Tyr Ile Ser Ser His Ile Tyr Gly Pro Met Glu Gln Asp Cys 180 185 190 Ser Met Ser Tyr Asn Asn Val Gly Gly Gly Trp Thr Asn Ile Asp His 195 200 205 Asn His His Tyr Ser Thr Pro Ala Tyr Asn Phe Leu Asp Arg Pro Met 210 215 220 Pro Leu Ser Gly Leu Glu Gly His His Gln Glu Glu Gly Glu Tyr Gly 225 230 235 240 Gly Asp Ala Tyr Leu Glu His Arg Arg Thr Leu Pro Leu Phe Pro Leu 245 250 255 His Gly Glu Asp His Ile Asn Gly Gly Gly Gly Ser Ile Trp Lys Tyr 260 265 270 Arg Gln Ser Asp Gly Cys Asp Arg Tyr Gly Arg Gly Pro Cys Ala Ser 275 280 285 Leu Lys Leu Cys Leu Asn 290 <210> 78 <211> 882 <212> DNA <213> Helianthus annuus <400> 78 atggaaactc aacaacaaca agcagatatt caagattttg ggaacaaaaa cagtaacacc 60 tatgcatgca ggcaaagcag tactaggtgg acacccacaa gtgatcagat cagaatactg 120 aaagagctct actacaacaa tggaatcaga tcaccaactg ctgatcagat tcagagaatc 180 gctgctcaac tcagacagta cggcaagatt gaaggcaaga acgtgtttta ttggtttcag 240 aaccataaag ctcgtgaacg ccagaagaaa cggttcactc ctactgctcc tccaccaccg 300 tctcacttct ccgatcatat taaccaccat cttcccaacg ctgcccccat gcagattccg 360 tctcaccacc accactacca ccatcaagaa ccaccacatg tctatgctca ccctcacaaa 420 ctctacacca ctcatcacat tggggttggt tcttcttcac aaggagtgat gggtgtaggg 480 tgtggctatg gatctgttgc tatggagaag agtttcagga agtgttcaat atcaccaccg 540 ggagagagta aggccacagg aggaattggc cggaatatcg gttcaagatc acggataagt 600 gtcgattcgt gttccttctt cgacacaatt aagccaaaga catatgagat gtttgagaat 660 catgatcaag atgaagaaca aggagagcct tcaactgaga tcgaaacact tccattattc 720 ccgattcacg acggcaacca tcatgatttc ttcggcatga ggacggcaaa tctgcaattg 780 gagcaaggca ctggaggtta ctacactgga ggaagctggt atcggtctga tgagagggcg 840 tctcttgagc tcagtctcaa ctcatatggt tattataact aa 882 <210> 79 <211> 293 <212> PRT <213> Helianthus annuus <400> 79 Met Glu Thr Gln Gln Gln Gln Ala Asp Ile Gln Asp Phe Gly Asn Lys 1 5 10 15 Asn Ser Asn Thr Tyr Ala Cys Arg Gln Ser Ser Thr Arg Trp Thr Pro 20 25 30 Thr Ser Asp Gln Ile Arg Ile Leu Lys Glu Leu Tyr Tyr Asn Asn Gly 35 40 45 Ile Arg Ser Pro Thr Ala Asp Gln Ile Gln Arg Ile Ala Ala Gln Leu 50 55 60 Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln 65 70 75 80 Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe Thr Pro Thr Ala 85 90 95 Pro Pro Pro Pro Ser His Phe Ser Asp His Ile Asn His His Leu Pro 100 105 110 Asn Ala Ala Pro Met Gln Ile Pro Ser His His His His Tyr His His 115 120 125 Gln Glu Pro Pro His Val Tyr Ala His Pro His Lys Leu Tyr Thr Thr 130 135 140 His His Ile Gly Val Gly Ser Ser Ser Gln Gly Val Met Gly Val Gly 145 150 155 160 Cys Gly Tyr Gly Ser Val Ala Met Glu Lys Ser Phe Arg Lys Cys Ser 165 170 175 Ile Ser Pro Pro Gly Glu Ser Lys Ala Thr Gly Gly Ile Gly Arg Asn 180 185 190 Ile Gly Ser Arg Ser Arg Ile Ser Val Asp Ser Cys Ser Phe Phe Asp 195 200 205 Thr Ile Lys Pro Lys Thr Tyr Glu Met Phe Glu Asn His Asp Gln Asp 210 215 220 Glu Glu Gln Gly Glu Pro Ser Thr Glu Ile Glu Thr Leu Pro Leu Phe 225 230 235 240 Pro Ile His Asp Gly Asn His His Asp Phe Phe Gly Met Arg Thr Ala 245 250 255 Asn Leu Gln Leu Glu Gln Gly Thr Gly Gly Tyr Tyr Thr Gly Gly Ser 260 265 270 Trp Tyr Arg Ser Asp Glu Arg Ala Ser Leu Glu Leu Ser Leu Asn Ser 275 280 285 Tyr Gly Tyr Tyr Asn 290 <210> 80 <211> 795 <212> DNA <213> Populus trichocarpa <400> 80 atggaacctc accaacaaca accaaatgag gataataacg gtggcgccaa agggaacttt 60 ctttgcaggc aaaccagtac aaggtggaat cccactactg accagataag aatattgaag 120 gaactgtact acatcaaagg tgttaggtcc ccaaatggag ctgagattca gcagatctct 180 gctaggctta gaaagtacgg caagattgaa ggcaagaatg tattttattg gtttcagaac 240 cataaagctc gtgagaggca aaagaaaaga ctcaccaatg aagtccccat gcaacaaaga 300 actgcctgga aacctgaaga ttattactct tacaagtact ccaacagtaa taataatcct 360 gggttttctt cggcttcttc atctgcaaat accggtgtgg ttactgttgg gcagacagat 420 agccatggat atggatcggt aaccatgcag gagaagaatt cttgggactg ttcagcacca 480 gctggtggta gcaatggtgc tggtagtgga tccatgagca atattaacta tggatcaggg 540 gttgacatta actctcacag ttcatcctat gctgtatttg gtcaagaaca agaagcagct 600 gcaaagattg agactcttcc tctatttcca atgcttggtg aggacatcag tagctctttc 660 aacatcaaca atatcaaccc agacttttat tacagtagcg gctgtggcta tggtgattat 720 ggcaatgaca cttcttcccg tacttccctg gaccttagtc tctactccta caatggccaa 780 ccacaagatt attaa 795 <210> 81 <211> 264 <212> PRT <213> Populus trichocarpa <400> 81 Met Glu Pro His Gln Gln Gln Pro Asn Glu Asp Asn Asn Gly Gly Ala 1 5 10 15 Lys Gly Asn Phe Leu Cys Arg Gln Thr Ser Thr Arg Trp Asn Pro Thr 20 25 30 Thr Asp Gln Ile Arg Ile Leu Lys Glu Leu Tyr Tyr Ile Lys Gly Val 35 40 45 Arg Ser Pro Asn Gly Ala Glu Ile Gln Gln Ile Ser Ala Arg Leu Arg 50 55 60 Lys Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn 65 70 75 80 His Lys Ala Arg Glu Arg Gln Lys Lys Arg Leu Thr Asn Glu Val Pro 85 90 95 Met Gln Gln Arg Thr Ala Trp Lys Pro Glu Asp Tyr Tyr Ser Tyr Lys 100 105 110 Tyr Ser Asn Ser Asn Asn Asn Pro Gly Phe Ser Ser Ala Ser Ser Ser 115 120 125 Ala Asn Thr Gly Val Val Thr Val Gly Gln Thr Asp Ser His Gly Tyr 130 135 140 Gly Ser Val Thr Met Gln Glu Lys Asn Ser Trp Asp Cys Ser Ala Pro 145 150 155 160 Ala Gly Gly Ser Asn Gly Ala Gly Ser Gly Ser Met Ser Asn Ile Asn 165 170 175 Tyr Gly Ser Gly Val Asp Ile Asn Ser His Ser Ser Ser Tyr Ala Val 180 185 190 Phe Gly Gln Glu Gln Glu Ala Ala Ala Lys Ile Glu Thr Leu Pro Leu 195 200 205 Phe Pro Met Leu Gly Glu Asp Ile Ser Ser Ser Phe Asn Ile Asn Asn 210 215 220 Ile Asn Pro Asp Phe Tyr Tyr Ser Ser Gly Cys Gly Tyr Gly Asp Tyr 225 230 235 240 Gly Asn Asp Thr Ser Ser Arg Thr Ser Leu Asp Leu Ser Leu Tyr Ser 245 250 255 Tyr Asn Gly Gln Pro Gln Asp Tyr 260 <210> 82 <211> 843 <212> DNA <213> Vitus vinifera <400> 82 atggaacctc aacaacagct ccagcagcag cagcaacaaa accaacaaca gccaaacgag 60 gatagcggca gcagcaaagg cagttttctg tgcaggcaaa gcagtacacg ctggactcct 120 acaactgacc agataagaat attgaaggac ctttactaca acaatggagt taggtcccca 180 agtgctgaac agattcagag gatctcagct aggctgaggc agtacggcaa gatcgaaggc 240 aagaacgtct tttattggtt tcagaaccat aaagctcgtg aaaggcagaa gaagcgattc 300 actactgata tgcccatgca aagatccctt ggaaatgccg gttggagacc tgatgatccc 360 attcacaaca aatttcatac cattcccact cctgggatat cttccccatc ttcttcttct 420 tcacccagtg tgcttgctgt tgggcagatg ggaagctttg gatatggatc tgtggagagg 480 agttttacag actgttcaat atcagcaggt ggtggtcgtg gtggggttgg tggatctata 540 aaccagagct tcgaatgggt tggcatggac ccatattctt catcttatgc actctttgac 600 aagagaaaaa caatgggtga aagcttcgaa gaagagcaag aagaagaagc aactccggag 660 attgaaacgc tcccactctt tcccatgcac gctgaggata tcactggctt ttgcaacatt 720 aagcccgaat ccgacgccta ctactccggc tggtacaggc ccgccgacgc caagaccagt 780 tcccgcactt ctcttgagct tagcctcaac tcctacgccg gcaggtcccc ggattccccc 840 tga 843 <210> 83 <211> 280 <212> PRT <213> Vitus vinifera <400> 83 Met Glu Pro Gln Gln Gln Leu Gln Gln Gln Gln Gln Gln Asn Gln Gln 1 5 10 15 Gln Pro Asn Glu Asp Ser Gly Ser Ser Lys Gly Ser Phe Leu Cys Arg 20 25 30 Gln Ser Ser Thr Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu 35 40 45 Lys Asp Leu Tyr Tyr Asn Asn Gly Val Arg Ser Pro Ser Ala Glu Gln 50 55 60 Ile Gln Arg Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly 65 70 75 80 Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln 85 90 95 Lys Lys Arg Phe Thr Thr Asp Met Pro Met Gln Arg Ser Leu Gly Asn 100 105 110 Ala Gly Trp Arg Pro Asp Asp Pro Ile His Asn Lys Phe His Thr Ile 115 120 125 Pro Thr Pro Gly Ile Ser Ser Pro Ser Ser Ser Ser Ser Pro Ser Val 130 135 140 Leu Ala Val Gly Gln Met Gly Ser Phe Gly Tyr Gly Ser Val Glu Arg 145 150 155 160 Ser Phe Thr Asp Cys Ser Ile Ser Ala Gly Gly Gly Arg Gly Gly Val 165 170 175 Gly Gly Ser Ile Asn Gln Ser Phe Glu Trp Val Gly Met Asp Pro Tyr 180 185 190 Ser Ser Ser Tyr Ala Leu Phe Asp Lys Arg Lys Thr Met Gly Glu Ser 195 200 205 Phe Glu Glu Glu Gln Glu Glu Glu Ala Thr Pro Glu Ile Glu Thr Leu 210 215 220 Pro Leu Phe Pro Met His Ala Glu Asp Ile Thr Gly Phe Cys Asn Ile 225 230 235 240 Lys Pro Glu Ser Asp Ala Tyr Tyr Ser Gly Trp Tyr Arg Pro Ala Asp 245 250 255 Ala Lys Thr Ser Ser Arg Thr Ser Leu Glu Leu Ser Leu Asn Ser Tyr 260 265 270 Ala Gly Arg Ser Pro Asp Ser Pro 275 280 <210> 84 <211> 879 <212> DNA <213> Arabidopsis thaliana <400> 84 atggagcctc ctcagcacca acatcaccac catcaggccg atcaagagtc cgggaacaac 60 aacaacaaca agtccggttc cggagggtac acttgcagac agacctccac cagatggaca 120 cctaccactg agcagatcaa gatcctgaag gagttgtact acaacaatgc catccgctcc 180 cctactgctg atcagattca gaagatcacc gctcgcctgc gtcaattcgg taagatcgag 240 ggtaagaacg tcttctactg gttccagaac cacaaggctc gtgagaggca gaagaagcgt 300 ttcaacggga ctaacatgac cactccttcc tcatcaccca attctgttat gatggccgcc 360 aacgaccact accatccttt gcttcatcac caccacggag ttcctatgca aagacccgca 420 aactccgtca acgtgaagtt gaaccaggac catcacctct accaccacaa caagccctac 480 ccctctttca acaacgggaa tctcaaccat gcctcctctg gaaccgaatg tggagttgtc 540 aacgcctcca acggctacat gtcttcccac gtgtacggct ctatggagca agattgctcc 600 atgaactaca acaacgttgg aggcggatgg gcaaacatgg atcaccacta ctcctccgct 660 ccctacaact tcttcgatag ggctaaacct ctcttcggct tggagggaca ccaagaagaa 720 gaggaatgcg gaggtgatgc ttacttggaa cacagaagga ctctccctct ctttccaatg 780 cacggtgagg accacatcaa cggaggatct ggcgctatct ggaagtatgg acagtccgag 840 gtcagaccat gcgcttccct ggaattgaga ctgaactag 879 <210> 85 <211> 292 <212> PRT <213> Arabidopsis thaliana <400> 85 Met Glu Pro Pro Gln His Gln His His His His Gln Ala Asp Gln Glu 1 5 10 15 Ser Gly Asn Asn Asn Asn Asn Lys Ser Gly Ser Gly Gly Tyr Thr Cys 20 25 30 Arg Gln Thr Ser Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile Lys Ile 35 40 45 Leu Lys Glu Leu Tyr Tyr Asn Asn Ala Ile Arg Ser Pro Thr Ala Asp 50 55 60 Gln Ile Gln Lys Ile Thr Ala Arg Leu Arg Gln Phe Gly Lys Ile Glu 65 70 75 80 Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg 85 90 95 Gln Lys Lys Arg Phe Asn Gly Thr Asn Met Thr Thr Pro Ser Ser Ser 100 105 110 Pro Asn Ser Val Met Met Ala Ala Asn Asp His Tyr His Pro Leu Leu 115 120 125 His His His His Gly Val Pro Met Gln Arg Pro Ala Asn Ser Val Asn 130 135 140 Val Lys Leu Asn Gln Asp His His Leu Tyr His His Asn Lys Pro Tyr 145 150 155 160 Pro Ser Phe Asn Asn Gly Asn Leu Asn His Ala Ser Ser Gly Thr Glu 165 170 175 Cys Gly Val Val Asn Ala Ser Asn Gly Tyr Met Ser Ser His Val Tyr 180 185 190 Gly Ser Met Glu Gln Asp Cys Ser Met Asn Tyr Asn Asn Val Gly Gly 195 200 205 Gly Trp Ala Asn Met Asp His His Tyr Ser Ser Ala Pro Tyr Asn Phe 210 215 220 Phe Asp Arg Ala Lys Pro Leu Phe Gly Leu Glu Gly His Gln Glu Glu 225 230 235 240 Glu Glu Cys Gly Gly Asp Ala Tyr Leu Glu His Arg Arg Thr Leu Pro 245 250 255 Leu Phe Pro Met His Gly Glu Asp His Ile Asn Gly Gly Ser Gly Ala 260 265 270 Ile Trp Lys Tyr Gly Gln Ser Glu Val Arg Pro Cys Ala Ser Leu Glu 275 280 285 Leu Arg Leu Asn 290 <210> 86 <211> 879 <212> DNA <213> Lotus japonicus <400> 86 atggaacaac ctccccaaca gcagcaacaa aacgaagacg gaggcggatc aagtggggga 60 aaaggagggt tcctcagtag gcagtccagt actaggtgga cacccaccac agaccagatt 120 cgcatcctca aggagctcta ctacaacaac ggaatacgct caccctccgc agaacagatc 180 cagaggatct ccgcaagact cagacagtac ggcaagatcg agggcaagaa cgtcttctac 240 tggttccaaa accacaaagc ccgcgaaagg cagaagaagc gcttcaccag tgagacacac 300 ttgctgccca acccaattca gcagcaacag cgcaacggaa caaacgcagc ttggaagcag 360 ccagaggagc aacccatcat caaccacacc aagtactcct ccaacaacat atccgcaccc 420 gcagggatta tcacaagtgc cccatcttct tccgcagaaa tagtctccgt ggggcagatc 480 ggaaacttcg gatacggatc tctgccaatg gagaagtcct tcagggactg cagtatctca 540 gccggaggta acacaggata cgccgggtct gctattaacc acaacctcgg atggatggga 600 gtcgacccat actcctctgc ttacaccaac ttcttcgaca aaatacgccc aacagaggag 660 gagactatgg aggaagaagg acaggagaac ggctcaccag aaatcgagac cctgcctttg 720 ttcccaatgc acggagagga catccactcc ggctacttca acctcaagag taactcatcc 780 cactacgctg gcggatggta ccaaactgag gaaggctact tcaacaacgg atcacgcgcc 840 agtctggagc tgtccctgaa gtaccctgac ttcgcttag 879 <210> 87 <211> 292 <212> PRT <213> Lotus japonicus <400> 87 Met Glu Gln Pro Pro Gln Gln Gln Gln Gln Asn Glu Asp Gly Gly Gly 1 5 10 15 Ser Ser Gly Gly Lys Gly Gly Phe Leu Ser Arg Gln Ser Ser Thr Arg 20 25 30 Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Glu Leu Tyr Tyr 35 40 45 Asn Asn Gly Ile Arg Ser Pro Ser Ala Glu Gln Ile Gln Arg Ile Ser 50 55 60 Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr 65 70 75 80 Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe Thr 85 90 95 Ser Glu Thr His Leu Leu Pro Asn Pro Ile Gln Gln Gln Gln Arg Asn 100 105 110 Gly Thr Asn Ala Ala Trp Lys Gln Pro Glu Glu Gln Pro Ile Ile Asn 115 120 125 His Thr Lys Tyr Ser Ser Asn Asn Ile Ser Ala Pro Ala Gly Ile Ile 130 135 140 Thr Ser Ala Pro Ser Ser Ser Ala Glu Ile Val Ser Val Gly Gln Ile 145 150 155 160 Gly Asn Phe Gly Tyr Gly Ser Leu Pro Met Glu Lys Ser Phe Arg Asp 165 170 175 Cys Ser Ile Ser Ala Gly Gly Asn Thr Gly Tyr Ala Gly Ser Ala Ile 180 185 190 Asn His Asn Leu Gly Trp Met Gly Val Asp Pro Tyr Ser Ser Ala Tyr 195 200 205 Thr Asn Phe Phe Asp Lys Ile Arg Pro Thr Glu Glu Glu Thr Met Glu 210 215 220 Glu Glu Gly Gln Glu Asn Gly Ser Pro Glu Ile Glu Thr Leu Pro Leu 225 230 235 240 Phe Pro Met His Gly Glu Asp Ile His Ser Gly Tyr Phe Asn Leu Lys 245 250 255 Ser Asn Ser Ser His Tyr Ala Gly Gly Trp Tyr Gln Thr Glu Glu Gly 260 265 270 Tyr Phe Asn Asn Gly Ser Arg Ala Ser Leu Glu Leu Ser Leu Lys Tyr 275 280 285 Pro Asp Phe Ala 290 <210> 88 <211> 909 <212> DNA <213> Medicago truncatula <400> 88 atggaacaac ctcagcagca gcagcaacca cagacccaac agcactctcc aaacaacgga 60 atcatggggt ccagacagag ttccaccaga tggaccccaa caaccgacca gatccgcatt 120 ctcaaagacc tctactacaa caacggaata aggtctccaa gtgccgagca aatccagagg 180 atctccgcaa ggctcagaca gtacgggaag atcgagggca agaacgtctt ctactggttc 240 caaaaccaca aagccagaga gcgccagaaa aagcgcttca cctccgacgt caacgtcgtc 300 cctatcatcc agcgcgcccc caacaacaat actatcataa gtgccgccaa ctggaagcct 360 gaccaccacg aacaacagca gaacattaat gtccacacaa accactccac atacaacatc 420 tcctccgctg gcctcagttc tgcttcctgc tcctcagcag aaatggtcac tgtggggcaa 480 ataggcaact acggctacgg gtctgtccca atggagaagt ctttccgcga atgcaccatc 540 tcagctggat gctcatcatc ccaggttggc tcaaccataa acccccacat cggctggatc 600 ggacaccacg tcgacccata ttcaagtgcc tacgctaacc tcttcgagaa aatacgcccc 660 aacgaggaga tcatggaaga gtacgaccag ggacaggaaa acggttcccc agagatcgag 720 acactcccac tcttccctat gcacggagaa gacatccacg gagggtattg caacctcaaa 780 tcaaattcct ccaactacgg aggatggtac caggcagaag acgcagggtt catgtacgga 840 agtcgtacca cttctctcga gctcagtctg aactcctacg gatgcaggtc ccccgactac 900 gccaattaa 909 <210> 89 <211> 302 <212> PRT <213> Medicago truncatula <400> 89 Met Glu Gln Pro Gln Gln Gln Gln Gln Pro Gln Thr Gln Gln His Ser 1 5 10 15 Pro Asn Asn Gly Ile Met Gly Ser Arg Gln Ser Ser Thr Arg Trp Thr 20 25 30 Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp Leu Tyr Tyr Asn Asn 35 40 45 Gly Ile Arg Ser Pro Ser Ala Glu Gln Ile Gln Arg Ile Ser Ala Arg 50 55 60 Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe 65 70 75 80 Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe Thr Ser Asp 85 90 95 Val Asn Val Val Pro Ile Ile Gln Arg Ala Pro Asn Asn Asn Thr Ile 100 105 110 Ile Ser Ala Ala Asn Trp Lys Pro Asp His His Glu Gln Gln Gln Asn 115 120 125 Ile Asn Val His Thr Asn His Ser Thr Tyr Asn Ile Ser Ser Ala Gly 130 135 140 Leu Ser Ser Ala Ser Cys Ser Ser Ala Glu Met Val Thr Val Gly Gln 145 150 155 160 Ile Gly Asn Tyr Gly Tyr Gly Ser Val Pro Met Glu Lys Ser Phe Arg 165 170 175 Glu Cys Thr Ile Ser Ala Gly Cys Ser Ser Ser Gln Val Gly Ser Thr 180 185 190 Ile Asn Pro His Ile Gly Trp Ile Gly His His Val Asp Pro Tyr Ser 195 200 205 Ser Ala Tyr Ala Asn Leu Phe Glu Lys Ile Arg Pro Asn Glu Glu Ile 210 215 220 Met Glu Glu Tyr Asp Gln Gly Gln Glu Asn Gly Ser Pro Glu Ile Glu 225 230 235 240 Thr Leu Pro Leu Phe Pro Met His Gly Glu Asp Ile His Gly Gly Tyr 245 250 255 Cys Asn Leu Lys Ser Asn Ser Ser Asn Tyr Gly Gly Trp Tyr Gln Ala 260 265 270 Glu Asp Ala Gly Phe Met Tyr Gly Ser Arg Thr Thr Ser Leu Glu Leu 275 280 285 Ser Leu Asn Ser Tyr Gly Cys Arg Ser Pro Asp Tyr Ala Asn 290 295 300 <210> 90 <211> 924 <212> DNA <213> Petunia x hybrida <400> 90 atggaaacag cacagcacca gcagaacaac caacagcact acctccacca acacctctcc 60 atcggacaag gaacaaacat cgaggacggc agtaacaaga acaactcctc caacttcatg 120 tgcaggcaaa atagtacccg ctggacacca accactgacc agatcaggat attaaaggac 180 ctctactaca acaacggagt cagatcacct accgctgaac agatccagag aatctccgcc 240 aagctcaggc agtacggcaa gatcgagggc aagaacgtct tctactggtt ccagaaccat 300 aaagcccgcg agagacaaaa gaagcgcctc atcgccgcag ccaccaccga taacactaac 360 ctcccaatgc agatgcagtt ccagagagga gtctggagat cctctgcaga cgaccccatc 420 caccacaagt atactaatcc aggagtgcac tgcccttcag cttcttccca cggagttctc 480 gcagtcggac agaacggcaa tcacggatac ggagccttgg caatggaaaa atccttccgc 540 gactgctcca tcagtccagg cagttccatg tcacaccacc accaccaaaa cttcgcttgg 600 gcaggagtcg atccttattc ctcaaccaca acctaccctt tcctcgagaa gaccaagcac 660 ttcgagaatg aaaccctcga agctgatgaa gagcagcaag aggaggacca ggagaactac 720 tactaccaaa ggaccacctc tgccatcgaa accctgcctc tcttccctat gcacgaggag 780 aacatctcct ccttctgcaa cctcaagcac caggagtcat ccggaggatt ctacaccgag 840 tggtacagag cagacgacaa cctcgcagca gccagagctt ccctcgagct ctccttgaac 900 tccttcatcg gcaactcctc ctag 924 <210> 91 <211> 307 <212> PRT <213> Petunia x hybrida <400> 91 Met Glu Thr Ala Gln His Gln Gln Asn Asn Gln Gln His Tyr Leu His 1 5 10 15 Gln His Leu Ser Ile Gly Gln Gly Thr Asn Ile Glu Asp Gly Ser Asn 20 25 30 Lys Asn Asn Ser Ser Asn Phe Met Cys Arg Gln Asn Ser Thr Arg Trp 35 40 45 Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp Leu Tyr Tyr Asn 50 55 60 Asn Gly Val Arg Ser Pro Thr Ala Glu Gln Ile Gln Arg Ile Ser Ala 65 70 75 80 Lys Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp 85 90 95 Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Leu Ile Ala 100 105 110 Ala Ala Thr Thr Asp Asn Thr Asn Leu Pro Met Gln Met Gln Phe Gln 115 120 125 Arg Gly Val Trp Arg Ser Ser Ala Asp Asp Pro Ile His His Lys Tyr 130 135 140 Thr Asn Pro Gly Val His Cys Pro Ser Ala Ser Ser His Gly Val Leu 145 150 155 160 Ala Val Gly Gln Asn Gly Asn His Gly Tyr Gly Ala Leu Ala Met Glu 165 170 175 Lys Ser Phe Arg Asp Cys Ser Ile Ser Pro Gly Ser Ser Met Ser His 180 185 190 His His His Gln Asn Phe Ala Trp Ala Gly Val Asp Pro Tyr Ser Ser 195 200 205 Thr Thr Thr Tyr Pro Phe Leu Glu Lys Thr Lys His Phe Glu Asn Glu 210 215 220 Thr Leu Glu Ala Asp Glu Glu Gln Gln Glu Glu Asp Gln Glu Asn Tyr 225 230 235 240 Tyr Tyr Gln Arg Thr Thr Ser Ala Ile Glu Thr Leu Pro Leu Phe Pro 245 250 255 Met His Glu Glu Asn Ile Ser Ser Phe Cys Asn Leu Lys His Gln Glu 260 265 270 Ser Ser Gly Gly Phe Tyr Thr Glu Trp Tyr Arg Ala Asp Asp Asn Leu 275 280 285 Ala Ala Ala Arg Ala Ser Leu Glu Leu Ser Leu Asn Ser Phe Ile Gly 290 295 300 Asn Ser Ser 305 <210> 92 <211> 864 <212> DNA <213> Phaseolus vulgaris <400> 92 atggaaccac agcagcccca gggcagtcaa ccaaacgagg acggtggaag tgggaaaggc 60 ggatttctct ccagacagtc ctccactagg tggactccaa ccactgacca gatcagaata 120 ctcaaagacc tctactacaa caacggaatc agatcccctt ccgcagaaca gatccaaagg 180 atctccgcta ggctcagaca gtacgggaag atcgagggca agaacgtctt ctactggttc 240 caaaaccaca aggccaggga gagacagaag aagaggttca cctccgacaa cgtccccatg 300 caaagacctg caccaacaaa tgctgcacct ccttggaaac ccgaccaaga ccccattcac 360 accaagtact ccaacatctc ctccacagga atctccagtg catcctcctc ctcagtcgag 420 atgatcaccg tcggacaaat ggggaactac gggtacggaa gtgtgccaat ggaaaagtcc 480 ttcagagact gctccatatc cgccggagga tcttccggtc atgttggaat aaaccacaac 540 ctcggctggg tgggagtcga tccttactct tctgcatacg ccaacttctt cgataaaata 600 agaccaaacg aagagaccct cgaggaagaa gaagaggagg aggaggagga agaagaagag 660 gacggaggag cagaaattga gaccctcccc ctgttcccaa tgcacggaga ggacattcac 720 ggctactgca acctcaagtc caacagttac aactacgacg gcaacggctg gtatcacagt 780 gaggatggct tcaaaaacgg cagtagggcc tccctcgagc tctccctcaa ctcttacacc 840 agaagatccc ccgacttcgc atag 864 <210> 93 <211> 287 <212> PRT <213> Phaseolus vulgaris <400> 93 Met Glu Pro Gln Gln Pro Gln Gly Ser Gln Pro Asn Glu Asp Gly Gly 1 5 10 15 Ser Gly Lys Gly Gly Phe Leu Ser Arg Gln Ser Ser Thr Arg Trp Thr 20 25 30 Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp Leu Tyr Tyr Asn Asn 35 40 45 Gly Ile Arg Ser Pro Ser Ala Glu Gln Ile Gln Arg Ile Ser Ala Arg 50 55 60 Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe 65 70 75 80 Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe Thr Ser Asp 85 90 95 Asn Val Pro Met Gln Arg Pro Ala Pro Thr Asn Ala Ala Pro Pro Trp 100 105 110 Lys Pro Asp Gln Asp Pro Ile His Thr Lys Tyr Ser Asn Ile Ser Ser 115 120 125 Thr Gly Ile Ser Ser Ala Ser Ser Ser Ser Val Glu Met Ile Thr Val 130 135 140 Gly Gln Met Gly Asn Tyr Gly Tyr Gly Ser Val Pro Met Glu Lys Ser 145 150 155 160 Phe Arg Asp Cys Ser Ile Ser Ala Gly Gly Ser Ser Gly His Val Gly 165 170 175 Ile Asn His Asn Leu Gly Trp Val Gly Val Asp Pro Tyr Ser Ser Ala 180 185 190 Tyr Ala Asn Phe Phe Asp Lys Ile Arg Pro Asn Glu Glu Thr Leu Glu 195 200 205 Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Asp Gly Gly Ala 210 215 220 Glu Ile Glu Thr Leu Pro Leu Phe Pro Met His Gly Glu Asp Ile His 225 230 235 240 Gly Tyr Cys Asn Leu Lys Ser Asn Ser Tyr Asn Tyr Asp Gly Asn Gly 245 250 255 Trp Tyr His Ser Glu Asp Gly Phe Lys Asn Gly Ser Arg Ala Ser Leu 260 265 270 Glu Leu Ser Leu Asn Ser Tyr Thr Arg Arg Ser Pro Asp Phe Ala 275 280 285 <210> 94 <211> 963 <212> DNA <213> Zea mays <400> 94 atggcggcca acgtgggcgc gggcaggagt gctggcggcg gcggagccgg cactggcact 60 ggcactgctg ctggcagcgg cggcgtgtcg acggccgtgt gccgccctag cggctcgcgg 120 tggacgccga cgccggagca gatcaggatc ctcaaggagc tctactacgg ctgcggcatc 180 cggtcgccca actcggagca gatccagcgc atcaccgcca tgctgcggca gcacggcaag 240 atcgagggca agaacgtctt ctactggttc cagaaccaca aggcccgcga gcgccagaag 300 cgccgcctca ccaacctcga cgtcaacgtg cccgtcgccg ccgacgacag cgcccaccgc 360 cttggcgtcc tctcgttgtc gccttcttca ggttgttcag gcgcggcgcc tccgtcgccc 420 accctcggct tctacgccgg cggcaatggc tccgctgtga tgctggacac gagttccgat 480 tggggcagcg ctgctgccat ggccactgag gcatgcttca tgcaggacta catgggcgtg 540 atgggcggcg cgtcaccgtg ggcatgctcc tcctcgtcgt cggaggaccc gatggcggcg 600 ctggcgctgg cgccgaaggt gacccgggcg cccgagacgc tccctctctt cccgaccggc 660 ggcggcggag acgataggca gcccccgcgg ccgcggcagt ctgtcccagc aggcgaggcc 720 atccgcggcg gcagcagcag cagcagctac cttccgttct ggggtgccgc gcccacccca 780 actggcagtg ccacttccgt tgcgatccag cagcaacacc agctgatgca gatgcaagag 840 cagtacagct tttacagcaa cgcccagctg ctgcccggca ccggcagcca ggatgcagca 900 gcaacatccc tggagctgag cctcagctcc tggtgctccc cttaccctgc agggaccatg 960 tga 963 <210> 95 <211> 320 <212> PRT <213> Zea mays <400> 95 Met Ala Ala Asn Val Gly Ala Gly Arg Ser Ala Gly Gly Gly Gly Ala 1 5 10 15 Gly Thr Gly Thr Gly Thr Ala Ala Gly Ser Gly Gly Val Ser Thr Ala 20 25 30 Val Cys Arg Pro Ser Gly Ser Arg Trp Thr Pro Thr Pro Glu Gln Ile 35 40 45 Arg Ile Leu Lys Glu Leu Tyr Tyr Gly Cys Gly Ile Arg Ser Pro Asn 50 55 60 Ser Glu Gln Ile Gln Arg Ile Thr Ala Met Leu Arg Gln His Gly Lys 65 70 75 80 Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg 85 90 95 Glu Arg Gln Lys Arg Arg Leu Thr Asn Leu Asp Val Asn Val Pro Val 100 105 110 Ala Ala Asp Asp Ser Ala His Arg Leu Gly Val Leu Ser Leu Ser Pro 115 120 125 Ser Ser Gly Cys Ser Gly Ala Ala Pro Pro Ser Pro Thr Leu Gly Phe 130 135 140 Tyr Ala Gly Gly Asn Gly Ser Ala Val Met Leu Asp Thr Ser Ser Asp 145 150 155 160 Trp Gly Ser Ala Ala Ala Met Ala Thr Glu Ala Cys Phe Met Gln Asp 165 170 175 Tyr Met Gly Val Met Gly Gly Ala Ser Pro Trp Ala Cys Ser Ser Ser 180 185 190 Ser Ser Glu Asp Pro Met Ala Ala Leu Ala Leu Ala Pro Lys Val Thr 195 200 205 Arg Ala Pro Glu Thr Leu Pro Leu Phe Pro Thr Gly Gly Gly Gly Asp 210 215 220 Asp Arg Gln Pro Pro Arg Pro Arg Gln Ser Val Pro Ala Gly Glu Ala 225 230 235 240 Ile Arg Gly Gly Ser Ser Ser Ser Ser Tyr Leu Pro Phe Trp Gly Ala 245 250 255 Ala Pro Thr Pro Thr Gly Ser Ala Thr Ser Val Ala Ile Gln Gln Gln 260 265 270 His Gln Leu Met Gln Met Gln Glu Gln Tyr Ser Phe Tyr Ser Asn Ala 275 280 285 Gln Leu Leu Pro Gly Thr Gly Ser Gln Asp Ala Ala Ala Thr Ser Leu 290 295 300 Glu Leu Ser Leu Ser Ser Trp Cys Ser Pro Tyr Pro Ala Gly Thr Met 305 310 315 320 <210> 96 <211> 978 <212> DNA <213> Zea mays <400> 96 atggcggcca atgcgggcgg cggtggagcg ggaggaggca gcggcagcgg cagcgtggct 60 gcgccggcgg tgtgccgccc cagcggctcg cggtggacgc cgacgccgga gcagatcagg 120 atgctgaagg agctctacta cggctgcggc atccggtcgc ccagctcgga gcagatccag 180 cgcatcaccg ccatgctgcg gcagcacggc aagatcgagg gcaagaacgt cttctactgg 240 ttccagaacc acaaggcccg cgagcgccag aagcgccgcc tcaccagcct cgacgtcaac 300 gtgcccgccg ccggcgcggc cgacgccacc accagccaac tcggcgtcct ctcgctgtcg 360 tcgccgcctt caggcgcggc gcctccctcg cccaccctcg gcttctacgc cgccggcaat 420 ggcggcggat cggctgggct gctggacacg agttccgact ggggcagcag cggcgctgcc 480 atggccaccg agacatgctt cctgcaggac tacatgggcg tgacggacac gggcagctcg 540 tcgcagtggc catgcttctc gtcgtcggac acgataatgg cggcggcggc ggccgcggcg 600 cgggtggcga cgacgcgggc gcccgagaca ctccctctct tcccgacctg cggcgacgac 660 gacgacgacg acagccagcc cccgccgcgg ccgcggcacg cagtcccagt cccggcaggc 720 gagaccatcc gcggcggcgg cggcagcagc agcagctact tgccgttctg gggtgccggt 780 gccgcgtcca caactgccgg cgccacttct tccgttgcga tccagcagca acaccagctg 840 caggagcagt acagctttta cagcaacagc acccagctgg ccggcaccgg cagccaagac 900 gtatcggctt cagcggccgc cctggagctg agcctcagct catggtgctc cccttaccct 960 gctgcaggga gcatgtga 978 <210> 97 <211> 325 <212> PRT <213> Zea mays <400> 97 Met Ala Ala Asn Ala Gly Gly Gly Gly Ala Gly Gly Gly Ser Gly Ser 1 5 10 15 Gly Ser Val Ala Ala Pro Ala Val Cys Arg Pro Ser Gly Ser Arg Trp 20 25 30 Thr Pro Thr Pro Glu Gln Ile Arg Met Leu Lys Glu Leu Tyr Tyr Gly 35 40 45 Cys Gly Ile Arg Ser Pro Ser Ser Glu Gln Ile Gln Arg Ile Thr Ala 50 55 60 Met Leu Arg Gln His Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp 65 70 75 80 Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Arg Arg Leu Thr Ser 85 90 95 Leu Asp Val Asn Val Pro Ala Ala Gly Ala Ala Asp Ala Thr Thr Ser 100 105 110 Gln Leu Gly Val Leu Ser Leu Ser Ser Pro Pro Ser Gly Ala Ala Pro 115 120 125 Pro Ser Pro Thr Leu Gly Phe Tyr Ala Ala Gly Asn Gly Gly Gly Ser 130 135 140 Ala Gly Leu Leu Asp Thr Ser Ser Asp Trp Gly Ser Ser Gly Ala Ala 145 150 155 160 Met Ala Thr Glu Thr Cys Phe Leu Gln Asp Tyr Met Gly Val Thr Asp 165 170 175 Thr Gly Ser Ser Ser Gln Trp Pro Cys Phe Ser Ser Ser Asp Thr Ile 180 185 190 Met Ala Ala Ala Ala Ala Ala Ala Arg Val Ala Thr Thr Arg Ala Pro 195 200 205 Glu Thr Leu Pro Leu Phe Pro Thr Cys Gly Asp Asp Asp Asp Asp Asp 210 215 220 Ser Gln Pro Pro Pro Arg Pro Arg His Ala Val Pro Val Pro Ala Gly 225 230 235 240 Glu Thr Ile Arg Gly Gly Gly Gly Ser Ser Ser Ser Tyr Leu Pro Phe 245 250 255 Trp Gly Ala Gly Ala Ala Ser Thr Thr Ala Gly Ala Thr Ser Ser Val 260 265 270 Ala Ile Gln Gln Gln His Gln Leu Gln Glu Gln Tyr Ser Phe Tyr Ser 275 280 285 Asn Ser Thr Gln Leu Ala Gly Thr Gly Ser Gln Asp Val Ser Ala Ser 290 295 300 Ala Ala Ala Leu Glu Leu Ser Leu Ser Ser Trp Cys Ser Pro Tyr Pro 305 310 315 320 Ala Ala Gly Ser Met 325 <210> 98 <211> 666 <212> DNA <213> Zea mays <400> 98 atggaggcgc tgagcgggcg ggtaggcgtc aagtgcgggc ggtggaaccc tacggcggag 60 caggtgaagg tcctgacgga gctcttccgc gcggggctgc ggacgcccag cacggagcag 120 atccagcgca tctccaccca cctcagcgcc ttcggcaagg tggagagcaa gaacgtcttc 180 tactggttcc agaaccacaa ggcccgcgag cgccaccacc acaagaagcg acgccgcggc 240 gcgtcgtcgt cctcccccga cagcggcagc ggcaggggaa gcaacaacga ggaagacggc 300 cgtggtgccg cctcgcagtc gcacgacgcc gacgccgacg ccgacctcgt gctgcaaccg 360 ccagagagca agcgggaggc cagaagctat ggccaccatc accggctcgt gacatgctac 420 gtcagggacg tggtggagca gcaggaggcg tcgccgtcgt gggagcggcc gacgagggag 480 gtggagacgc tagagctctt ccccctcaag tcgtacggcg acctcgaggc ggcggagaag 540 gtccggtcgt acgtcagagg aagcggcgcc accagcgagc agtgcaggga gttgtccttc 600 ttcgacgtcg tctccgccgg ccgggatccg ccgctcgagc tcaggctctg cagcttcggt 660 ccctag 666 <210> 99 <211> 221 <212> PRT <213> Zea mays <400> 99 Met Glu Ala Leu Ser Gly Arg Val Gly Val Lys Cys Gly Arg Trp Asn 1 5 10 15 Pro Thr Ala Glu Gln Val Lys Val Leu Thr Glu Leu Phe Arg Ala Gly 20 25 30 Leu Arg Thr Pro Ser Thr Glu Gln Ile Gln Arg Ile Ser Thr His Leu 35 40 45 Ser Ala Phe Gly Lys Val Glu Ser Lys Asn Val Phe Tyr Trp Phe Gln 50 55 60 Asn His Lys Ala Arg Glu Arg His His His Lys Lys Arg Arg Arg Gly 65 70 75 80 Ala Ser Ser Ser Ser Pro Asp Ser Gly Ser Gly Arg Gly Ser Asn Asn 85 90 95 Glu Glu Asp Gly Arg Gly Ala Ala Ser Gln Ser His Asp Ala Asp Ala 100 105 110 Asp Ala Asp Leu Val Leu Gln Pro Pro Glu Ser Lys Arg Glu Ala Arg 115 120 125 Ser Tyr Gly His His His Arg Leu Val Thr Cys Tyr Val Arg Asp Val 130 135 140 Val Glu Gln Gln Glu Ala Ser Pro Ser Trp Glu Arg Pro Thr Arg Glu 145 150 155 160 Val Glu Thr Leu Glu Leu Phe Pro Leu Lys Ser Tyr Gly Asp Leu Glu 165 170 175 Ala Ala Glu Lys Val Arg Ser Tyr Val Arg Gly Ser Gly Ala Thr Ser 180 185 190 Glu Gln Cys Arg Glu Leu Ser Phe Phe Asp Val Val Ser Ala Gly Arg 195 200 205 Asp Pro Pro Leu Glu Leu Arg Leu Cys Ser Phe Gly Pro 210 215 220 <210> 100 <211> 975 <212> DNA <213> Zea mays <400> 100 atggagacgc cacagcagca atccgccgcc gccgccgccg ccgccgccca cgggcaggac 60 gacggcgggt cgccgccgat gtcgccggcc tccgccgcgg cggcggcgct ggcgaacgcg 120 cggtggaacc cgaccaagga gcaggtggcc gtgctggagg ggctgtacga gcacggcctg 180 cgcaccccca gcgcggagca gatacagcag atcacgggca ggctgcggga gcacggcgcc 240 atcgagggca agaacgtctt ctactggttc cagaaccaca aggcccgcca gcgccagagg 300 cagaagcagg acagcttcgc ctacttcagc aggctcctcc gccggccccc gccgctgccc 360 gtgctctcca tgccccccgc gccaccgtac catcacgccc gcgtcccggc gccgcccgcg 420 ataccgatgc cgatggcgcc gccgccgccc gctgcatgca acgacaacgg cggcgcgcgt 480 gtgatctaca ggaacccatt ctacgtggct gcgccgcagg cgccccctgc aaatgccgcc 540 tactactacc cacagccaca gcagcagcag cagcagcagg tgacagtcat gtaccagtac 600 ccgagaatgg aggtagccgg ccaggacaag atgatgacca gggccgcggc gcaccagcag 660 cagcagcaca acggcgccgg gcaacaaccg ggacgcgccg gccaccccag ccgcgagacg 720 ctccagctgt tcccgctcca gcccaccttc gtgctgcggc acgacaaggg gcgcgccgcc 780 aacggcagta ataacgactc cctgacgtcg acgtcgacgg cgactgcgac agcgacagcg 840 acagcgacag cgtccgcttc catctccgag gactcggatg gcctggagag cggcagctcc 900 ggcaagggcg tcgaggaggc gcccgcgctg ccgttctatg acttcttcgg gctccagtcc 960 tccggaggcc gctga 975 <210> 101 <211> 324 <212> PRT <213> Zea mays <400> 101 Met Glu Thr Pro Gln Gln Gln Ser Ala Ala Ala Ala Ala Ala Ala Ala 1 5 10 15 His Gly Gln Asp Asp Gly Gly Ser Pro Pro Met Ser Pro Ala Ser Ala 20 25 30 Ala Ala Ala Ala Leu Ala Asn Ala Arg Trp Asn Pro Thr Lys Glu Gln 35 40 45 Val Ala Val Leu Glu Gly Leu Tyr Glu His Gly Leu Arg Thr Pro Ser 50 55 60 Ala Glu Gln Ile Gln Gln Ile Thr Gly Arg Leu Arg Glu His Gly Ala 65 70 75 80 Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg 85 90 95 Gln Arg Gln Arg Gln Lys Gln Asp Ser Phe Ala Tyr Phe Ser Arg Leu 100 105 110 Leu Arg Arg Pro Pro Pro Leu Pro Val Leu Ser Met Pro Pro Ala Pro 115 120 125 Pro Tyr His His Ala Arg Val Pro Ala Pro Pro Ala Ile Pro Met Pro 130 135 140 Met Ala Pro Pro Pro Pro Ala Ala Cys Asn Asp Asn Gly Gly Ala Arg 145 150 155 160 Val Ile Tyr Arg Asn Pro Phe Tyr Val Ala Ala Pro Gln Ala Pro Pro 165 170 175 Ala Asn Ala Ala Tyr Tyr Tyr Pro Gln Pro Gln Gln Gln Gln Gln Gln 180 185 190 Gln Val Thr Val Met Tyr Gln Tyr Pro Arg Met Glu Val Ala Gly Gln 195 200 205 Asp Lys Met Met Thr Arg Ala Ala Ala His Gln Gln Gln Gln His Asn 210 215 220 Gly Ala Gly Gln Gln Pro Gly Arg Ala Gly His Pro Ser Arg Glu Thr 225 230 235 240 Leu Gln Leu Phe Pro Leu Gln Pro Thr Phe Val Leu Arg His Asp Lys 245 250 255 Gly Arg Ala Ala Asn Gly Ser Asn Asn Asp Ser Leu Thr Ser Thr Ser 260 265 270 Thr Ala Thr Ala Thr Ala Thr Ala Thr Ala Thr Ala Ser Ala Ser Ile 275 280 285 Ser Glu Asp Ser Asp Gly Leu Glu Ser Gly Ser Ser Gly Lys Gly Val 290 295 300 Glu Glu Ala Pro Ala Leu Pro Phe Tyr Asp Phe Phe Gly Leu Gln Ser 305 310 315 320 Ser Gly Gly Arg <210> 102 <211> 543 <212> DNA <213> Zea mays <400> 102 atgcatggtg tacgtacgta tctcttaatt ttcattgtat caaagctgca ggtgcaagtg 60 ccgccgacga cgacgacggt gggtggcacg acgcggtgga acccgtcgcc ggaccagata 120 agggtgctgg agatgctgta ccgcgggggg atgcgcacgc ccaactcgtt ccagatcgag 180 cagatcacgg aggagctcgg caagtacggc cggatcgagg gcaagaacgt cttctactgg 240 ttccagaacc acaaggcccg cgagcgccag aagcagaagc gagctgccct cctcaccctc 300 agcaccacca ccactgcttc cacgctgcta ccaccagctg ctgaaaccaa ggagggagtg 360 gagacgaaaa aagaagaagc gtgtgaagat gcatcgagcc gcaagcggag gtgcagggcc 420 tgggaagatg tcgtcgtcca tggtggcggc gacgatgccg gtacggaggt agctgacgac 480 tactacaccg acgacgatgt gaccctggag ctcttcccgc tgcgtcctga tcaggggaaa 540 taa 543 <210> 103 <211> 180 <212> PRT <213> Zea mays <400> 103 Met His Gly Val Arg Thr Tyr Leu Leu Ile Phe Ile Val Ser Lys Leu 1 5 10 15 Gln Val Gln Val Pro Pro Thr Thr Thr Thr Val Gly Gly Thr Thr Arg 20 25 30 Trp Asn Pro Ser Pro Asp Gln Ile Arg Val Leu Glu Met Leu Tyr Arg 35 40 45 Gly Gly Met Arg Thr Pro Asn Ser Phe Gln Ile Glu Gln Ile Thr Glu 50 55 60 Glu Leu Gly Lys Tyr Gly Arg Ile Glu Gly Lys Asn Val Phe Tyr Trp 65 70 75 80 Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Gln Lys Arg Ala Ala 85 90 95 Leu Leu Thr Leu Ser Thr Thr Thr Thr Ala Ser Thr Leu Leu Pro Pro 100 105 110 Ala Ala Glu Thr Lys Glu Gly Val Glu Thr Lys Lys Glu Glu Ala Cys 115 120 125 Glu Asp Ala Ser Ser Arg Lys Arg Arg Cys Arg Ala Trp Glu Asp Val 130 135 140 Val Val His Gly Gly Gly Asp Asp Ala Gly Thr Glu Val Ala Asp Asp 145 150 155 160 Tyr Tyr Thr Asp Asp Asp Val Thr Leu Glu Leu Phe Pro Leu Arg Pro 165 170 175 Asp Gln Gly Lys 180 <210> 104 <211> 708 <212> DNA <213> Zea mays <400> 104 atggacacgg aggcgccgag cgggcgggtg ggcgtcaagt gcgggcggtg gaacccgacg 60 ccggagcagg tgaaggtcct gacggagctc ttccgcgcgg ggctgcggac gcccagcacg 120 gaccagatcc agcgcatctc cgcccacctc ggcgccttcg gcaaggtgga gagcaagaac 180 gtcttctact ggttccagaa ccacaaggcc cgcgagcgcc accaccacaa gaagaagaag 240 cgccgccgac gcggcgcggc aatggcatcg tcgtcctccc ccgacagcgg cagcggcagc 300 gggagcagcg aggaagacgg ccgtgttgcc gccgccgccg acgccgacct cgtgctgcaa 360 ccatcgccgg gaagaagcaa aagcaaacgg gaggccagaa gaagctacgg cggcggccac 420 catcgccggc tcgttacatg taagcagcta gcgcacacac atcgctacgt cagggacgtt 480 gtggagcagc aggaggagga cacgtgggag cggccgacga gggaggcggt ggagactctg 540 gagctcttcc ccctcaagtc gtacgtcgac ctcgaggcgg agaaggtccg gcggtacgtc 600 gtcagggccg gcgccagcga gcagtgcagg gacttctcct tcttcgacgt gtccggcggt 660 cgggatccgc cgcttcttga gcttaggctc tgcagcttcg gtccctag 708 <210> 105 <211> 235 <212> PRT <213> Zea mays <400> 105 Met Asp Thr Glu Ala Pro Ser Gly Arg Val Gly Val Lys Cys Gly Arg 1 5 10 15 Trp Asn Pro Thr Pro Glu Gln Val Lys Val Leu Thr Glu Leu Phe Arg 20 25 30 Ala Gly Leu Arg Thr Pro Ser Thr Asp Gln Ile Gln Arg Ile Ser Ala 35 40 45 His Leu Gly Ala Phe Gly Lys Val Glu Ser Lys Asn Val Phe Tyr Trp 50 55 60 Phe Gln Asn His Lys Ala Arg Glu Arg His His His Lys Lys Lys Lys 65 70 75 80 Arg Arg Arg Arg Gly Ala Ala Met Ala Ser Ser Ser Ser Pro Asp Ser 85 90 95 Gly Ser Gly Ser Gly Ser Ser Glu Glu Asp Gly Arg Val Ala Ala Ala 100 105 110 Ala Asp Ala Asp Leu Val Leu Gln Pro Ser Pro Gly Arg Ser Lys Ser 115 120 125 Lys Arg Glu Ala Arg Arg Ser Tyr Gly Gly Gly His His Arg Arg Leu 130 135 140 Val Thr Cys Lys Gln Leu Ala His Thr His Arg Tyr Val Arg Asp Val 145 150 155 160 Val Glu Gln Gln Glu Glu Asp Thr Trp Glu Arg Pro Thr Arg Glu Ala 165 170 175 Val Glu Thr Leu Glu Leu Phe Pro Leu Lys Ser Tyr Val Asp Leu Glu 180 185 190 Ala Glu Lys Val Arg Arg Tyr Val Val Arg Ala Gly Ala Ser Glu Gln 195 200 205 Cys Arg Asp Phe Ser Phe Phe Asp Val Ser Gly Gly Arg Asp Pro Pro 210 215 220 Leu Leu Glu Leu Arg Leu Cys Ser Phe Gly Pro 225 230 235 <210> 106 <211> 1545 <212> DNA <213> Zea mays <400> 106 atggcctcct ccttcaacaa caaaaccagt cactggccga gcatgtttag gtccaagcac 60 gccgctgagc cgtggcaggc gcagcctgac atcagcagct cgccgccgtc gctcctctca 120 ggtggcggct ctagcagcac caccaccatc ggccgctgcc tcaagcaccc cctctcaggt 180 tactcaggtg gcgaggagcg gaccccggat ccgaagcccc ggtggaaccc caggccggag 240 cagatccgga ttctggaggc catcttcaac tccggcatgg tcaacccgcc gcgcgacgag 300 atcccccgca tccgcatgcg cctgcaggag tacggccagg tcggcgacgc caacgtcttc 360 tactggttcc agaaccgcaa gtcccgctcc aagaacaagc agcgcaccgg acagctgggg 420 ctgggccttg cgcgcgcgcc gggttgcggg gccgctgcgc cgcccgtcac gccgcagccc 480 ttgatccaga accaattcca ggtgctcgcc tcgcccgcgc aggcgccagc ttcctcgtcc 540 tcgtcctcct ccgaccgctc ctctgggtcc agcaagccgg cgccgcagcc gatgtccgcg 600 acggcagcgg ccatgaactt tccagggccg ctcggcgcgg cgtgtgcgca gatgtactac 660 caagcccacc ccgtggcgcc ggtctcggcg ttgccagcgc acaaggtgca ggaccctgta 720 gcctccgacg agcccgtctt ccagccgtgg ccacaaggct acttcctgtc ggctgccgag 780 gtcgccagca ttctcggggg ccagtacagg cacgacgtgc ccgtgcagca gcagccaccg 840 gcgacgttgc ctgcgggagc gttcttgggg ctgtacaacg aggtgacaga gccgactgtc 900 actggtcaca ggacatgcgc ctggggcccg gcggggctcg ggcagttctg gcccgtgggc 960 ggcgctgatc accatcagca ccacaagcac aataccaccg cggcgaccaa caccgtagca 1020 agggacgcgg cgcacgagca cgccacgacg ctggggttgc tgcagtacgg cttcgaagct 1080 agcgcggcca tggagacggc ttccgcggcg gtgccccttg ctgcgtcgcc gggaaccgct 1140 gcgagcgtgg cgacggctgg gctcacatct ttgcctgcaa gtactaatgc tgttgtcgtc 1200 aactatgatc tgctgcaagg actggcagtg cccgggtccg ggtccggggc cgtcggtgtc 1260 agcaccggcg gtgcaccgcc tgtcgccgtt gccgcggcgc ccacggcagc gcaggagggc 1320 gtcgtcgtag cactgtgcat cacggacagc gtcacgggca agagcgtggc acacaacgtc 1380 gcggctgcca ggctcgacgt gagggcgcag ttcggcgagg cggccgttct gctccgcgcc 1440 gtcggcgacc gcggcgggct cgacctcgtg cccgtccccg tcgacgcgtt gggctgcaca 1500 gtggagccgc tccagcacgg tgccttttac tacgtgctgg tataa 1545 <210> 107 <211> 514 <212> PRT <213> Zea mays <400> 107 Met Ala Ser Ser Phe Asn Asn Lys Thr Ser His Trp Pro Ser Met Phe 1 5 10 15 Arg Ser Lys His Ala Ala Glu Pro Trp Gln Ala Gln Pro Asp Ile Ser 20 25 30 Ser Ser Pro Pro Ser Leu Leu Ser Gly Gly Gly Ser Ser Ser Thr Thr 35 40 45 Thr Ile Gly Arg Cys Leu Lys His Pro Leu Ser Gly Tyr Ser Gly Gly 50 55 60 Glu Glu Arg Thr Pro Asp Pro Lys Pro Arg Trp Asn Pro Arg Pro Glu 65 70 75 80 Gln Ile Arg Ile Leu Glu Ala Ile Phe Asn Ser Gly Met Val Asn Pro 85 90 95 Pro Arg Asp Glu Ile Pro Arg Ile Arg Met Arg Leu Gln Glu Tyr Gly 100 105 110 Gln Val Gly Asp Ala Asn Val Phe Tyr Trp Phe Gln Asn Arg Lys Ser 115 120 125 Arg Ser Lys Asn Lys Gln Arg Thr Gly Gln Leu Gly Leu Gly Leu Ala 130 135 140 Arg Ala Pro Gly Cys Gly Ala Ala Ala Pro Pro Val Thr Pro Gln Pro 145 150 155 160 Leu Ile Gln Asn Gln Phe Gln Val Leu Ala Ser Pro Ala Gln Ala Pro 165 170 175 Ala Ser Ser Ser Ser Ser Ser Ser Asp Arg Ser Ser Gly Ser Ser Lys 180 185 190 Pro Ala Pro Gln Pro Met Ser Ala Thr Ala Ala Ala Met Asn Phe Pro 195 200 205 Gly Pro Leu Gly Ala Ala Cys Ala Gln Met Tyr Tyr Gln Ala His Pro 210 215 220 Val Ala Pro Val Ser Ala Leu Pro Ala His Lys Val Gln Asp Pro Val 225 230 235 240 Ala Ser Asp Glu Pro Val Phe Gln Pro Trp Pro Gln Gly Tyr Phe Leu 245 250 255 Ser Ala Ala Glu Val Ala Ser Ile Leu Gly Gly Gln Tyr Arg His Asp 260 265 270 Val Pro Val Gln Gln Gln Pro Pro Ala Thr Leu Pro Ala Gly Ala Phe 275 280 285 Leu Gly Leu Tyr Asn Glu Val Thr Glu Pro Thr Val Thr Gly His Arg 290 295 300 Thr Cys Ala Trp Gly Pro Ala Gly Leu Gly Gln Phe Trp Pro Val Gly 305 310 315 320 Gly Ala Asp His His Gln His His Lys His Asn Thr Thr Ala Ala Thr 325 330 335 Asn Thr Val Ala Arg Asp Ala Ala His Glu His Ala Thr Thr Leu Gly 340 345 350 Leu Leu Gln Tyr Gly Phe Glu Ala Ser Ala Ala Met Glu Thr Ala Ser 355 360 365 Ala Ala Val Pro Leu Ala Ala Ser Pro Gly Thr Ala Ala Ser Val Ala 370 375 380 Thr Ala Gly Leu Thr Ser Leu Pro Ala Ser Thr Asn Ala Val Val Val 385 390 395 400 Asn Tyr Asp Leu Leu Gln Gly Leu Ala Val Pro Gly Ser Gly Ser Gly 405 410 415 Ala Val Gly Val Ser Thr Gly Gly Ala Pro Pro Val Ala Val Ala Ala 420 425 430 Ala Pro Thr Ala Ala Gln Glu Gly Val Val Val Ala Leu Cys Ile Thr 435 440 445 Asp Ser Val Thr Gly Lys Ser Val Ala His Asn Val Ala Ala Ala Arg 450 455 460 Leu Asp Val Arg Ala Gln Phe Gly Glu Ala Ala Val Leu Leu Arg Ala 465 470 475 480 Val Gly Asp Arg Gly Gly Leu Asp Leu Val Pro Val Pro Val Asp Ala 485 490 495 Leu Gly Cys Thr Val Glu Pro Leu Gln His Gly Ala Phe Tyr Tyr Val 500 505 510 Leu Val <210> 108 <211> 2000 <212> DNA <213> Glycine max <400> 108 tttaaatttg ttgcagaatg atcataatta aatttccaag gcaataaaaa tccctgtcta 60 caaatttatt tccatagggg ctttggatcg atggcgtgga ccccacctga aagtgacacc 120 cttggatctc acgggtttca gtgtcaaacc tcatttaata agtcacccca tctcccaccc 180 ttaatttcca ccatccaatt ctccaatctc cttcttcata aattaacggc tgtgatctat 240 ttgaccctac tactacttta tgaccattct ccattcggtt acaaatcaat atttctctca 300 ctcccaaatt aaaaaattgc caattaccaa acatgccatc tctcaaaaag tacaaagtgt 360 tactattatt tctatttaat tagtatagta gtactctttc taataaattt aatattaaaa 420 tcacataatt gtacgtacta cttagtaatt gagattcttc aaaataaaga gaataactct 480 caggtaattt agtgaattct gaaagacaat aaattgtgtt tttgacaaat gttaatacaa 540 caattttata cagtagtaat attttttata tcacaaaaac taaatataat ttcatctgaa 600 taatctattt attttttaat gtaaatattt atcacttaga ttatcaagat tacgattttt 660 aaagaaaaga ttaacaagat tacgaattgg gttaagccaa tttgtataag aaaattggat 720 atcatatgcc catgaggtct tgggtcctgt ccacaaggaa atttaactca tttttactct 780 atatatactt ctaacgtcat atattgcaac aataatatat gcgttacttt gacgaaattt 840 ctactaacgt tggcattgaa gttgctttta gaagtaccta tcaattttcc acgatatttc 900 agtcacattg aaacaacagt tgaattcaga aaaaaaaaat taaaaaaatt gaacttcaaa 960 tacttcacag tccaccttaa acatatcatt cacattcatc taagataaaa atcaatcact 1020 aattttaatt agataatagt attaatagaa acattaaaag ccttatcttt ttataaagct 1080 gtttttcatt ttcatcacat ccagcatcga tctcaatcca aactctttct tttcacaccc 1140 tatgagtaac tcaaacctga catcatccaa cacggcgcgt gtatcccact ctcttccttg 1200 gaagacgtaa ggaggcagga agcgcgtggg agaggaactg agaggaaaaa aaggtttgac 1260 tttggtgaag ggtgtgggaa acgtttagaa tggccattaa gaaagcacca cgtggagcgt 1320 aacagccgac gtagtaagta ttgctgatcg gtttctgtca acccgcattt accatttaca 1380 gtaaatttat taaattaaat taaattaacc catttattac aacacactat gaattgaatg 1440 aatgaatatg agcattatat gaagagagag aggaacgtag aaaagaaaac taggataagg 1500 taaaggacta aggagaaaaa gaaagagaaa gagagaaagg gactctgtcc ccttttgtgt 1560 gcgtgtgttt tgtcaactga gtgaactctc aagtgaacta acatcaccaa aaaaaccact 1620 ctcttgcaac ctcctcatca cattgctcct tctggtattc catataactt attaactttg 1680 atttttttct ctctttaatt ttttcttttc ttccgatcat gtaacatgct aaaaaataat 1740 ttgtaagatt ctggttggtt ggttttgtag ggaatctttt tctcttgttt ttttctgggt 1800 tgaatgagaa taatgttttg atagtttaaa gctttttatt tgtttggttt ctgatatggg 1860 aaagttttgt tgtggggtgt tagagacagg tttggctcaa ctgaagtaag gtttggttat 1920 cttattacct tatctctcac cacatttaaa aacacacaaa acacagttac acaagtgaag 1980 tgtggttttg ggaagcaaga 2000 <210> 109 <211> 2000 <212> DNA <213> Glycine max <400> 109 atgaattatt atatatgctc tatttgttaa attttttaaa ataattattt taaaagttat 60 acatagattt ttttattggt taatagtata aaattttaac tctcacaaaa caccatgcac 120 gaaaattaaa ctcatgaaca atataaatat acttttttat ctgaaataaa cgagggaaaa 180 tatctcttat ttgagaaaaa aaatttaaaa atatttttgt tactactatg tcataagtta 240 atatcttgga agtttaaaga atattaactt gttaaaataa ttcaatattt tttttgtctg 300 taatgtgtta gccagtatta acacaattaa tttgtttttg gtaataacaa tgcttagata 360 tggcaagaaa actcatactt gtgggtatcc gtctgaattc gtttcgactt tgacgggtaa 420 tacctgagtt gacctggtat aggttcgggt ttggattttt ctcgataatc aaaagtcaga 480 tacgggtatg ggtatgaggt tactagactc gtcccgaccc caaacccgaa ctcgccctgc 540 cacttaaaat ctttaaattt ttttgcataa tttaatcaaa aggcataaaa tttttattac 600 tagttaattt tattttagaa tttttacata atttaatatt attttcttaa ctatttttgt 660 agacacatgc gctataataa atttattaat atataagtag ttaaaacaaa tgtttaacaa 720 ttaactttct ttttctaaaa tcaaactttt aatatttttt ttataaaaaa aaatattttt 780 ttaatttaaa tcgtgtatgg gacagggtcg gggataccgg ataggtacag gaatgagaaa 840 ataaatttaa acccgatatc gaatacgaat atgaagatat attgagaagt tagaataaga 900 gattgagaga caatactcat aaccatccca ctctccattg caatgtctaa caatgcttac 960 acatgcacaa gcaatgactc agctcaggta acattcatac attctattaa catgttggcg 1020 tcacaagcat tattataata cacccaattt ttcctattaa atgaaaaaga cgacgtaccg 1080 tatgcccctc caattaaaca caaccgacat ggagaaaatg atgatggttg attgcctacg 1140 aaaggtgaca attcgtgaaa acaatgatga tatatgcttg ctaaaagaga aataccaata 1200 aaatgtttac acattattta ttattaactg aacattatat aaattgtaaa gagtttaata 1260 aatatatatt gttaagttga aagtttttgc agcattgttg cttgcacttc ttgtgcaaca 1320 attctgacct tatttataca ttatcaatca attgaaaata tacagtttat tatagatgat 1380 gattttaaaa tttaacaaat ttattataaa taatgattta ttagacaatg gtgtaaaaaa 1440 cttttatatt ttactaaaaa catgtttttt taaaatgtat gatgctatta ttcacgtttc 1500 tcatttttta acagtcggtt gaagactttt agtttagctg cttcacacta ccaaggccat 1560 tccttcccca ctttgaacaa ttcacatttt tattattttt tgcagtaccc atatttttca 1620 agtgacagcc tactaatcat tcaaaggtgt aaaaaattga atttttattg ctatccagca 1680 accacttttt tgtttttttt tttgtacaaa atatatagat accatatgtg acatgcctat 1740 aataaattaa taactgaagg ctgataaaat aaaaggttaa taactgaagg tagttttatc 1800 tacttgtctc tacgtcaaga agcacgtgaa cacaactgaa aggtgccaac aaaacatgtt 1860 ggaaggttag ctacataatc ttcggtggag caaatattcc acttgtgcac atgcatgcat 1920 atataattgg ttatttgcaa tgcctcattc catgcactga aactagttgc gacacttgta 1980 ttgcagtgca cataataacc 2000 <210> 110 <211> 2000 <212> DNA <213> Glycine max <400> 110 acttcgatgt tcttgcactt gttatagaat tcatgtcata ataagtgtgt gctcgaagta 60 aaaattaatt ttaattgttt tttctattta tacttctaac attagcaatt tgcataacaa 120 acaagattta atacaagatt aattttgtaa aattgttact ttttttattt attcttgcta 180 gaaaaagaga aagaaccttt cacgaaaatc ttcataattt catttgaggt tcacatatat 240 ctttggaagt ggaggactac gatgcaacag tttatttatt atttcagaga ataaccatta 300 cccaaaattt ctttgaccgg agtagcttgt ccatgaaata agacatggtg gtaataaaaa 360 aaaggattaa ttgaacatta ttgtcatgga atagtaaaca tacataaatt aaatgactct 420 tcatgtcttt ttttcttctt catcatgata ttcataggaa ataatcctca tttgactaat 480 tagttgggtc accaggtgta ccaatagtta ttggtctttt tctttttgag taacaaatac 540 cattgaaaag tcatttaatt tattatttgt atgtataatg gtagatgatc tggaggttat 600 tattatatct ttttcgggtt tgtgttaagt ttatttattt ttcttaataa atgattcatt 660 acaaaaggat ttttttaagt gtatatttct tctttttttt ctacttcttc tttcaagggc 720 aggaaaatct catatattgt ggtccttcct tcaccactcc catgtggatg gttcacgggt 780 tcataattac tcacgcttac ttagccaaca tttagatatg actctatcta aatttttttg 840 tttacttcaa catttctcac ttgtcctact tttgttatcg aataattttt ggatattaaa 900 tggaccttcc cagaagttaa ttttaatgtg gccaatttct tctcttttgt aattagctct 960 tagacaataa taataatgat atttctagat tatctccatt tattttccta ttcatacgtg 1020 attctgttgt gcgattgaat gttagataga cggtgtagat gaggtgcaaa attttaattc 1080 agtctgacat gtttaattgt gaagaaaatg ttgaaaaaaa ttaaaataaa agaaaagaaa 1140 taaatgagaa gagaatgcaa agtcttgaat taaataaaaa aatattaata gcaaaataat 1200 tttaattaac accacatgac tatatcatac aataaatata aggaaaaaat aaataagaaa 1260 aataattatt tagcctgggt tgaagcacta gttgattttg accgtgtgta gcaaaatgat 1320 ccctctccta agatacgaca accagttggt ttcgaccgac gtgtgcagca aaagaatccc 1380 gaaacttgtg aacactaatg cttttgctct aaaaaataag tttttatagg aaaagaaaaa 1440 gaagatattt gggagaaagg agttatgtgc ttgtgttgtt cttttttaga gaacaagaaa 1500 tatatatata tatagagaga gagagagaga tagatcacct tgtgcagcaa acaaaaatat 1560 gatcattgta ttcaacctat agttattaaa acaaacgtaa tagaccagtt gactcattaa 1620 aataaaatct taagaaaatt taaaataaat attttatttt ataaaataaa attaatatat 1680 atttataaat tttaaattaa aacacagata cttatcgata gaaaatagta atagtgtgta 1740 ggatattaat gaaaggtgaa aggctttttt gttcaccccc aagacttgtt gatgttgagt 1800 cgttggtaac aatacacacc acctaacacc atcacgtgct caccttcaat ttacaatact 1860 cgaacgaaac gatttgttag attaagtaac tgaaagatta atgttattat ttaagaagct 1920 tctctcctga gggaggatta aaaaactgtc tataaagtaa gtttcatttt atcctttgaa 1980 gtgtgtgaga gagtgaaaac 2000 <210> 111 <211> 10324 <212> DNA <213> Artificial Sequence <220> <223> PHP80728 T-DNA <400> 111 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccgtcg acggatccgt accatcaact ttgtataata aagttgcccg 1320 gacctctaga tatcggatcc cggggttaac cttcagctta gaaatgatgt gttttctaca 1380 cttttctctt cttttttttc ttttcatctc acatgaatca aaaagagaaa gcttaattac 1440 attaaaggtt gatgtgatat ttactttcta aatatttgct agaagaaatt ataatgataa 1500 cattaaatta agttatagtc atttctattt cggttgtctc ccactttctg ctatgctttt 1560 acaacaacag ttcaagattt ggaatcgttt tctttctcca ccaatcgtag taatacttct 1620 ttttgtgttt gttgaaattt tgcaagattc tctcttcctc tggttaatac acataactta 1680 cttggtaagc ttggctgcat tttcttttca agcttttaat caaagactca ctagaaattg 1740 caaaggcaaa cataaaatta tgtgatcaaa ctcctactgt gagtttttgg ttacaaacaa 1800 aagttaaaaa caaaagcttg atatttaaaa gataaactca acacatatgc atttgactgg 1860 cgcacagaag acaattcaag acttgggaaa acagtgagcc atcaaaatcg tcagataatt 1920 cagggaatat attgaatgtg gagatgtggt aatcacacta ttgcacacaa agagtggcaa 1980 acaagcggcg cccttggttt ggtatagtaa gagacaattg ttttggactg tttgtgttgc 2040 catattcaca aaaatactat tgagctatgt atgttaaaca ttatggtcaa tgagtagtag 2100 tgaacattca agtttttttt tttttccact tcaagttttt catcttatta ttatttttcg 2160 ttgtgatgca gtagtacggc ccatcttcac cagtagtata tcaaagccga aacagatgac 2220 aaattgttta acatgcactt ggattattat tgagagttga aatacaaagg cccgttacaa 2280 gcccaaacga aggtacataa aagaaaagca gaaaatgaaa acgaaatcga tagggataag 2340 tttttccaac tgacggaaac acaaactaca ccaggtgaaa tagatcacct cccaagtgca 2400 tgagacacga accaaccact tatcatcaga tcacaaaaga gctcaaagat ctagcgatcg 2460 cctaattcag tttgtggcca agctttgatg gcaagtcgca atatctagcg acggccacct 2520 cgtgctgctc cacataagtc tccttgtcgg cctcttttat gcgctcgagt ctatggtcca 2580 cgtaatacac gccgggcatt ttcaagtttt tggcaggctt tttggagcgg taggtggtct 2640 tgaagttaca gatgagatgg cctccgccga caagcttcaa ggccatgtcg gatctacctt 2700 caagaccacc gtcggctggg taaagcattt cagtattggc ttcccagccg agagtcttct 2760 tctgcatcac gggaccgttt gatgggaagt tcacgccgcg gatcttgacg ttgtagatca 2820 agcagccgtc ttgaagacta gtgtcttggg tggcggtcaa gactcctccg tcttcgtagg 2880 tggtcactct ctcccaggtg aatccctctg ggaatgactg tttgaagaag tctgggatgc 2940 cctgggtgtg gttgatgaag gttctactcc cgtacatgaa ggaggtggct agtatgtcga 3000 aggcgaatgg gagtggacct ccttcgacca ccttgattct catagtctgg gttccctcat 3060 agggcttgcc ctcaccctca gaggtacact tgaagtggtg attattcacg gtgccctcca 3120 tgtataactt catgtgcatg ttctctttaa tgagctcctc ccccttggac accatggctg 3180 tagagtcaac aatcacaaga taaatcagaa tcaaaatcca tcacgtaatt tcaagaagca 3240 atttcgtgaa aaaaaatcct aatctatggc cacagaatca acaaataagg tagttgctaa 3300 aggacctgag caatatctct tgaaatacca tacagaatca acgaataagg cttttatcaa 3360 cggatctgaa caatttcttg aaaaacgact gaagtatcaa caaataaggc tgttgtttaa 3420 cggatctgaa gtagttcatg aaaaaaacct taatctacga ctacagaatc aacaattaag 3480 gcttgttgta aacggatctg aaaatcttta tgaaaaaaac tctaattaga cctaatttac 3540 ggttataaaa tcatcaatta aggacgttct tgcattaata aaagctgaat cttctgatta 3600 tgaaaattta taatcgatga aacgatagaa aacacaatca aatcaacatc atgctgatgt 3660 agaacaaaca ataaagaaga agaaaaaaaa acggaagaaa tctacgaacc ttgaaaggag 3720 aggtagggaa ctgcgcaaag agaatgaact aatttgattg caattgaagt gcgattaggg 3780 tcgaggggtg tgggggcatt ttatagcata tgcggtggaa ccgttctcca atcatattgt 3840 gacgcgtgcc catgtatggt gtgacttggt gtttatgaca cagatgatat ttcggggagt 3900 ccttcgtagt tgacggtttc gtaatcttga cggagtcaga gatgtacctt caccgcttca 3960 cgcttggctt tatccgttac cacggcgtta ttaaattggg cccgcacccg ttgcaagtta 4020 ggaccacagt gtccattacc tgaacggtcg gctgctacca gtattttaca ttattaaaat 4080 ctatggaaaa gctgtcacgc tagatttttt tttttttttt tatggataag aaaagactaa 4140 taggaaagct ttcttactcc actaaagtct ttaataatta ttttttcaat tttcaaataa 4200 aatcataatc atcccactaa attctttctc atttgttttt cactttgtaa gatattcttc 4260 cgtagatttt tataagtaat tctttaagat acatagcata ataaattgta tacttttagt 4320 tacagtctat ataaaattag tcatttaaaa aatatagcct taatgatttt tttaaaatga 4380 agttattttt ttctaatttt tctttcttat ttattatcga atgatttttt ctactaatcg 4440 tttagggaag agttttattt ttctaatgac gagatgtgtt aaatgatgca acttgaaggc 4500 gacatatttt ttaccaataa aataaaacta aaagatagct tgttgacaaa aacaataatt 4560 gatgttaaag aaaacgtaag ggcaatgggt atcatctaat tgttggacaa gttgtcttct 4620 aaggctaggt aagcttttgt tttttattag ctgaaatatt tatttaattg ttctataaat 4680 aaatttttta aaatgatttt taatgttttt aaatattatt taaaataatt tttttaaaat 4740 tttaacattt tagtttttaa ttatttttta tcttacttaa attaattata atttattatt 4800 ttctaccatt ttatactttc caattaattt tatcgaagca ttaataccat ctggtatttt 4860 gtcactgctt cgtatgattg tttattaaat tttagcttct ttcacattgg agatgctggg 4920 cggtgtgagt tacagcatgg tgtgaagtta aggtggtttg attatggaac ccgaaggtgt 4980 tggtgcaagg tggataaaat tgcttgccag gagatacata aaatgattta caaaatgaaa 5040 taaagaataa ataaatagtc aagttcaaat ttaaatcaag gattagaatt atttttccgt 5100 acatgatgta accgcaatca ccccctgctg aatccagatg gccattctgg ccgaatttaa 5160 gtgactaggg tcaccctgct gaatccagat ggccattctg gccgaattta agtgactagg 5220 gtcacgtgac cctagtcact taccggattc tggccgtacc gagctcgaat tcggcccaac 5280 ttttctatac aaagttgagg tacgagatcc gcccatccgg ccggccagat cctgcaggag 5340 atccaagctt ggcgcgccgc ggacctgaat tccagcacac tggcggccgt tactagtaac 5400 ggccgccagt gtgctggaat tcgcccttcc caagctttgc tctagatcaa actcacatcc 5460 aaacataaca tggatatctt ccttaccaat catactaatt attttgggtt aaatattaat 5520 cattattttt aagatattaa ttaagaaatt aaaagatttt ttaaaaaaat gtataaaatt 5580 atattattca tgatttttca tacatttgat tttgataata aatatatttt ttttaatttc 5640 ttaaaaaatg ttgcaagaca cttattagac atagtcttgt tctgtttaca aaagcattca 5700 tcatttaata cattaaaaaa tatttaatac taacagtaga atcttcttgt gagtggtgtg 5760 ggagtaggca acctggcatt gaaacgagag aaagagagtc agaaccagaa gacaaataaa 5820 aagtatgcaa caaacaaatc aaaatcaaag ggcaaaggct ggggttggct caattggttg 5880 ctacattcaa ttttcaactc agtcaacggt tgagattcac tctgacttcc ccaatctaag 5940 ccgcggatgc aaacggttga atctaaccca caatccaatc tcgttactta ggggcttttc 6000 cgtcattaac tcacccctgc cacccggttt ccctataaat tggaactcaa tgctcccctc 6060 taaactcgta tcgcttcaga gttgagacca agacacactc gttcatatat ctctctgctc 6120 ttctcttctc ttctacctct caaggtactt ttcttctccc tctaccaaat cctagattcc 6180 gtggttcaat ttcggatctt gcacttctgg tttgctttgc cttgcttttt cctcaactgg 6240 gtccatctag gatccatgtg aaactctact ctttctttaa tatctgcgga atacgcgttt 6300 gactttcaga tctagtcgaa atcatttcat aattgccttt ctttctttta gcttatgaga 6360 aataaaatca cttttttttt atttcaaaat aaaccttggg ccttgtgctg actgagatgg 6420 ggtttggtga ttacagaatt ttagcgaatt ttgtaattgt acttgtttgt ctgtagtttt 6480 gttttgtttt cttgtttctc atacattcct taggcttcaa ttttattcga gtataggtca 6540 caataggaat tcaaactttg agcaggggaa ttaatccctt ccttcaaatc cagtttgttt 6600 gtatatatgt ttaaaaaatg aaacttttgc tttaaattct attataactt tttttatggc 6660 tgaaattttt gcatgtgtct ttgctctctg ttgtaaattt actgtttagg tactaactct 6720 aggcttgttg tgcagttttt gaagtataac aacagaagtt cctattccga agttcctatt 6780 ctctagaaag tataggaact tccaccacac aacacaatgg cggccaccgc ttccagaacc 6840 acccgattct cttcttcctc ttcacacccc accttcccca aacgcattac tagatccacc 6900 ctccctctct ctcatcaaac cctcaccaaa cccaaccacg ctctcaaaat caaatgttcc 6960 atctccaaac cccccacggc ggcgcccttc accaaggaag cgccgaccac ggagcccttc 7020 gtgtcacggt tcgcctccgg cgaacctcgc aagggcgcgg acatccttgt ggaggcgctg 7080 gagaggcagg gcgtgacgac ggtgttcgcg taccccggcg gtgcgtcgat ggagatccac 7140 caggcgctca cgcgctccgc cgccatccgc aacgtgctcc cgcgccacga gcagggcggc 7200 gtcttcgccg ccgaaggcta cgcgcgttcc tccggcctcc ccggcgtctg cattgccacc 7260 tccggccccg gcgccaccaa cctcgtgagc ggcctcgccg acgctttaat ggacagcgtc 7320 ccagtcgtcg ccatcaccgg ccaggtcgcc cgccggatga tcggcaccga cgccttccaa 7380 gaaaccccga tcgtggaggt gagcagatcc atcacgaagc acaactacct catcctcgac 7440 gtcgacgaca tcccccgcgt cgtcgccgag gctttcttcg tcgccacctc cggccgcccc 7500 ggtccggtcc tcatcgacat tcccaaagac gttcagcagc aactcgccgt gcctaattgg 7560 gacgagcccg ttaacctccc cggttacctc gccaggctgc ccaggccccc cgccgaggcc 7620 caattggaac acattgtcag actcatcatg gaggcccaaa agcccgttct ctacgtcggc 7680 ggtggcagtt tgaattccag tgctgaattg aggcgctttg ttgaactcac tggtattccc 7740 gttgctagca ctttaatggg tcttggaact tttcctattg gtgatgaata ttcccttcag 7800 atgctgggta tgcatggtac tgtttatgct aactatgctg ttgacaatag tgatttgttg 7860 cttgcctttg gggtaaggtt tgatgaccgt gttactggga agcttgaggc ttttgctagt 7920 agggctaaga ttgttcacat tgatattgat tctgccgaga ttgggaagaa caagcaggcg 7980 cacgtgtcgg tttgcgcgga tttgaagttg gccttgaagg gaattaatat gattttggag 8040 gagaaaggag tggagggtaa gtttgatctt ggaggttgga gagaagagat taatgtgcag 8100 aaacacaagt ttccattggg ttacaagaca ttccaggacg cgatttctcc gcagcatgct 8160 atcgaggttc ttgatgagtt gactaatgga gatgctattg ttagtactgg ggttgggcag 8220 catcaaatgt gggctgcgca gttttacaag tacaagagac cgaggcagtg gttgacctca 8280 gggggtcttg gagccatggg ttttggattg cctgcggcta ttggtgctgc tgttgctaac 8340 cctggggctg ttgtggttga cattgatggg gatggtagtt tcatcatgaa tgttcaggag 8400 ttggccacta taagagtgga gaatctccca gttaagatat tgttgttgaa caatcagcat 8460 ttgggtatgg tggttcagtt ggaggatagg ttctacaagt ccaatagagc tcacacctat 8520 cttggagatc cgtctagcga gagcgagata ttcccaaaca tgctcaagtt tgctgatgct 8580 tgtgggatac cggcagcgcg agtgacgaag aaggaagagc ttagagcggc aattcagaga 8640 atgttggaca cccctggccc ctaccttctt gatgtcattg tgccccatca ggagcatgtg 8700 ttgccgatga ttcccagtaa tggatccttc aaggatgtga taactgaggg tgatggtaga 8760 acgaggtact gattgcctag accaaatgtt ccttgatgct tgttttgtac aatatatata 8820 agataatgct gtcctagttg caggatttgg cctgtggtga gcatcatagt ctgtagtagt 8880 tttggtagca agacatttta ttttcctttt atttaactta ctacatgcag tagcatctat 8940 ctatctctgt agtctgatat ctcctgttgt ctgtattgtg ccgttggatt ttttgctgta 9000 gtgagactga aaatgatgtg ctagtaataa tatttctgtt agaaatctaa gtagagaatc 9060 tgttgaagaa gtcaaaagct aatggaatca ggttacatat tcaatgtttt tcttttttta 9120 gcggttggta gacgtgtaga ttcaacttct cttggagctc acctaggcaa tcagtaaaat 9180 gcatattcct tttttaactt gccatttatt tacttttagt ggaaattgtg accaatttgt 9240 tcatgtagaa cggatttgga ccattgcgtc cacaaaacgt ctcttttgct cgatcttcac 9300 aaagcgatac cgaaatccag agatagtttt caaaagtcag aaatggcaaa gttataaata 9360 gtaaaacaga atagatgctg taatcgactt caataacaag tggcatcacg tttctagttc 9420 tagacccatc agctgaggta caaattaatt aataagtgac tagggtcacg tgaccctagt 9480 cacttatgat tccgatgact tcgtaggttc ctagctcaag ccgctcgtgt ccaagcgtca 9540 cttacgatta gctaatgatt acggcatcta ggaccgacta gttcttatgt gcttctagtc 9600 tccaaatgtg gttgatagtt attttgctct aagatcaaca gtaatgaagt ataaatcatc 9660 gttgtggtgt gctactcggt taattgagca ttaacacaca caaacatgac gaggatggta 9720 taatctccaa aaatgtgtac tttgttaggt gggaccctat agccttgatt aatgtgctat 9780 gttaggcatg cctggaaacg tgtgacgcat atgttttgtg aacctgttga tattatatgt 9840 gcttttatat taccatattt tattaaaata ctaatattta ttactagtaa gatataacat 9900 tctatctagc ttaaaaacta accataaata ttccataata actagattta ccaaactaat 9960 atactaaata tacataataa atacaaaatt aacaagacaa taatcaatat ttatgagctt 10020 aatatattta gacattatgg ttggtcgacg ataatcatgc taacttttcg taattgcttg 10080 attgaaatat gcttagaata atgcctcttt gttctacatg gcaaataggg accattatgg 10140 tgtaacaccc tgggaaccac aaacaccccg aaatgctact aaactacaca actaaccttc 10200 atatataaaa tttcgacagc atctcctttg aaaatttgca tagacgtgga agcaacagag 10260 tataaacaga tctagctagc taagtttaaa cggccggcca tttacaattg aatatatcct 10320 gcca 10324 <210> 112 <211> 14348 <212> DNA <213> Artificial Sequence <220> <223> PHP80730 T-DNA <400> 112 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccgtcg acggatccgt accatcaact ttgtataata aagttgcccg 1320 cggccgccgg tacctctgtt aatgaacgta aggagcttct ggatgacggc tgggacttct 1380 ttgacaaaga gacagagctc taagtgtctt cttttcagta gattatcggt gctagaataa 1440 aagagatatg aagggattct gggttctgct ctatgtagag taaagcttgg atctttgatc 1500 taatctaccg ttagtataaa acatctattg agctctcttt tatttttctt ctcagtgtat 1560 agtttgctta ttgttttttt ggtgtttttt tccacctctt tgtggtactg acataaacaa 1620 agactatcgt tttgtcatag aattttgaga ttttgaagaa aagttggata atttagtata 1680 ctttttaact gactttataa aacgaattag cttcaattag agcttcacaa ttctcggatt 1740 ttactgaatg acaagttaac aatttaagct agaaaaaaat atgctctaag cctggacatg 1800 ttgttagtca tggccagcgt atgtgtacat taggtaaatg aaaacaaaaa caaaaacttt 1860 gaaggtttct gttaagaaag gaactaattt agcaataaga gacatgatat tacaagctca 1920 acactgtcgc tatatctacc agaagccaac aaatcttcca gtgcgtacca ttggagccac 1980 ttttcaggct cattagacaa aagtagcaca tataatgtaa agacgaaacg atgataaaga 2040 gactcacaag gacataattt caatacagaa gaagtttgat gtcattactc attagaaaga 2100 aaggtataac acaggaacgg aaacatagtt gaacaattat tcatcagggg ctttacaagg 2160 ccccaaaaca caaaccacca aagttttaaa tgaaacgaga catgaacttc ttctgattca 2220 taacagagat aagtttccta ggtcccaatc tagttcagtc tcaattccag ggaagcgcat 2280 ggtctgacct cggactgtcc atacttccag atagcgccag atcctccgtt gatgtggtcc 2340 tcaccgtgca ttggaaagag agggagagtc cttctgtgtt ccaagtaagc atcacctccg 2400 cattcctctt cttcttggtg tccctccaag ccgaagagag gtttagccct atcgaagaag 2460 ttgtagggag cggaggagta gtggtgatcc atgtttgccc atccgcctcc aacgttgttg 2520 tagttcatgg agcaatcttg ctccatagag ccgtacacgt gggaagacat gtagccgttg 2580 gaggcgttga caactccaca ttcggttcca gaggaggcat ggttgagatt cccgttgttg 2640 aaagaggggt agggcttgtt gtggtggtag aggtgatggt cctggttcaa cttcacgttg 2700 acggagtttg cgggtctttg cataggaact ccgtggtggt gatgaagcaa aggatggtag 2760 tggtcgttgg cggccatcat aacagaattg ggtgatgagg aaggagtggt catgttagtc 2820 ccgttgaaac gcttcttctg cctctcacga gccttgtggt tctggaacca gtagaagacg 2880 ttcttaccct cgatcttacc gaattgacgc aggcgagcgg tgatcttctg aatctgatca 2940 gcagtagggg agcggatggc attgttgtag tacaactcct tcaggatctt gatctgctca 3000 gtggtaggtg tccatctggt ggaggtctgt ctgcaagtgt accctccgga accggacttg 3060 ttgttgttgt tgttcccgga ctcttgatcg gcctgatggt ggtgatgttg gtgctgagga 3120 ggctccatgg actcattagc tagtaatagt attttctttg cttctgctat gtaatagtat 3180 ttcctttcct ttctgcccgg ccttatatat atatatatat cataccaccg acagaagaag 3240 aatatctgca tcctcattta atatttattg caacttccaa gccgtgaacc cgccggatat 3300 cctgccactt cttttttctg actcactgca tggtaacatg tggctcacac ctgtccacat 3360 ccaccagtaa aactccaccc acaaatatta ctctctctgg actcaaccat tagacaaaaa 3420 attagttaat attatttaat tttgataatt tcaataaaaa gttaatttat tccttaaaat 3480 atcatctata ttgattatat aagaaaaaaa aataagatta ttaaaaataa aactcaatta 3540 aacgaatgat gttttgaaaa tacatcatta agtaggtgat aaagtaggtg atagttaatt 3600 aaaatttgtt tatattttaa tctaacacaa ttgatttttt ttttattttt tttacatttg 3660 aggtctaaag ggagtattat gattaaagat ttttttgtgt aattatgtta gaattatcta 3720 tcaattttat tcataattaa ttttaacaaa actttattta aaaaatttga attcaatact 3780 atttggacta actacttgtt ggtagactct ttctttttac acattttttt tcatctctcc 3840 aaataatttt acaaatatgt aaaaagaaaa cataatttta taaataaaaa atgaaacaaa 3900 aattaatttc taaactgtat ttaatacatt aaatgctttt tcctgaaatg cattaaatac 3960 ttatttaata cattaaataa gtttctttaa tgcattaatt ttcaaaaatt aatttaatac 4020 atttaagtaa cttatttccg taattagtaa cagtcaaaat aataagtgag gtccttttta 4080 ttgatttttt ttattctagt ttctattttt caaatttttg tttttttatt ttatttcaga 4140 aaccagaaca catgctgaat taacaataac tataatatgc atatataaaa taatattcta 4200 tatatatata tatttaaata taccataaat actatttaat atataaatat tacgattata 4260 acaaatttaa tactctctat tgtcttaaat gtaagaaaaa agatcagcca gagataagtg 4320 gaatattcca attattttgt gagaaaatca tcatgcccac cagacaatag tagtagcgaa 4380 aagcaagaca ttctaacaca taaattgggt gtttgataat tctgtgtttt taagttagac 4440 ttggtagaga ggtgcgtatt gaagggttcc ctcgctgcac gttgtaaaac aattatatcc 4500 attctttatc aatatatata tatatatatt ttgtgtacaa gctttaaact tcaaaactta 4560 ctattcgaac tctaccactt gcctgacctt atacaaagaa gaagaattca gggtccttgg 4620 tcaaacccac actagtttct ccgtgtgtgt gtgtgtatac ggattgtttg attaacgata 4680 gaattgtcat ttacaagatt acatgacaaa aaatcaacta cttaacagtt aaacaccacg 4740 gagacattaa ttatctgaca ggcaccatat gaacaatcga aatgacaagt atcataaaca 4800 ttttttttag aaattcatga actcctgttt aaacgaaagc aatataaact gtactggggt 4860 gaatatgcca taaaaacagt aaactctaaa agatgctttt attgaaaaat aacttttaaa 4920 atgttgttta tacgtaaagg tttttggctt aaaaatgggt ttaactccta taaaatgagc 4980 gaaaattgat tttgtaaact tttctttgga atgaatccct gatcattatt tttgatccct 5040 attatcatgg ggaaacttgt atgtttaatg gaaggttaag taacccttcc aatttctaat 5100 tgatattcaa tagtggtttt ggggctatgt cccgggccat tctggcccac tttgtacaag 5160 aaagctgggt ctagatatct cgagtgcggc cgcgaattcg gtaccggatc cagtcgactg 5220 aattggttcc ggcgccagcc tgcttttttg tacaaacttg aagcttcggc cggggccgca 5280 ttcgcaaaac acacctagac tagatttgtt ttgctaaccc aattcgagct cggtacctcg 5340 cgaatgcatc tagatatcgg atcccggggt taaccttcag cttagaaatg atgtgttttc 5400 tacacttttc tcttcttttt tttcttttca tctcacatga atcaaaaaga gaaagcttaa 5460 ttacattaaa ggttgatgtg atatttactt tctaaatatt tgctagaaga aattataatg 5520 ataacattaa attaagttat agtcatttct atttcggttg tctcccactt tctgctatgc 5580 ttttacaaca acagttcaag atttggaatc gttttctttc tccaccaatc gtagtaatac 5640 ttctttttgt gtttgttgaa attttgcaag attctctctt cctctggtta atacacataa 5700 cttacttggt aagcttggct gcattttctt ttcaagcttt taatcaaaga ctcactagaa 5760 attgcaaagg caaacataaa attatgtgat caaactccta ctgtgagttt ttggttacaa 5820 acaaaagtta aaaacaaaag cttgatattt aaaagataaa ctcaacacat atgcatttga 5880 ctggcgcaca gaagacaatt caagacttgg gaaaacagtg agccatcaaa atcgtcagat 5940 aattcaggga atatattgaa tgtggagatg tggtaatcac actattgcac acaaagagtg 6000 gcaaacaagc ggcgcccttg gtttggtata gtaagagaca attgttttgg actgtttgtg 6060 ttgccatatt cacaaaaata ctattgagct atgtatgtta aacattatgg tcaatgagta 6120 gtagtgaaca ttcaagtttt tttttttttc cacttcaagt ttttcatctt attattattt 6180 ttcgttgtga tgcagtagta cggcccatct tcaccagtag tatatcaaag ccgaaacaga 6240 tgacaaattg tttaacatgc acttggatta ttattgagag ttgaaataca aaggcccgtt 6300 acaagcccaa acgaaggtac ataaaagaaa agcagaaaat gaaaacgaaa tcgataggga 6360 taagtttttc caactgacgg aaacacaaac tacaccaggt gaaatagatc acctcccaag 6420 tgcatgagac acgaaccaac cacttatcat cagatcacaa aagagctcaa agatctagcg 6480 atcgcctaat tcagtttgtg gccaagcttt gatggcaagt cgcaatatct agcgacggcc 6540 acctcgtgct gctccacata agtctccttg tcggcctctt ttatgcgctc gagtctatgg 6600 tccacgtaat acacgccggg cattttcaag tttttggcag gctttttgga gcggtaggtg 6660 gtcttgaagt tacagatgag atggcctccg ccgacaagct tcaaggccat gtcggatcta 6720 ccttcaagac caccgtcggc tgggtaaagc atttcagtat tggcttccca gccgagagtc 6780 ttcttctgca tcacgggacc gtttgatggg aagttcacgc cgcggatctt gacgttgtag 6840 atcaagcagc cgtcttgaag actagtgtct tgggtggcgg tcaagactcc tccgtcttcg 6900 taggtggtca ctctctccca ggtgaatccc tctgggaatg actgtttgaa gaagtctggg 6960 atgccctggg tgtggttgat gaaggttcta ctcccgtaca tgaaggaggt ggctagtatg 7020 tcgaaggcga atgggagtgg acctccttcg accaccttga ttctcatagt ctgggttccc 7080 tcatagggct tgccctcacc ctcagaggta cacttgaagt ggtgattatt cacggtgccc 7140 tccatgtata acttcatgtg catgttctct ttaatgagct cctccccctt ggacaccatg 7200 gctgtagagt caacaatcac aagataaatc agaatcaaaa tccatcacgt aatttcaaga 7260 agcaatttcg tgaaaaaaaa tcctaatcta tggccacaga atcaacaaat aaggtagttg 7320 ctaaaggacc tgagcaatat ctcttgaaat accatacaga atcaacgaat aaggctttta 7380 tcaacggatc tgaacaattt cttgaaaaac gactgaagta tcaacaaata aggctgttgt 7440 ttaacggatc tgaagtagtt catgaaaaaa accttaatct acgactacag aatcaacaat 7500 taaggcttgt tgtaaacgga tctgaaaatc tttatgaaaa aaactctaat tagacctaat 7560 ttacggttat aaaatcatca attaaggacg ttcttgcatt aataaaagct gaatcttctg 7620 attatgaaaa tttataatcg atgaaacgat agaaaacaca atcaaatcaa catcatgctg 7680 atgtagaaca aacaataaag aagaagaaaa aaaaacggaa gaaatctacg aaccttgaaa 7740 ggagaggtag ggaactgcgc aaagagaatg aactaatttg attgcaattg aagtgcgatt 7800 agggtcgagg ggtgtggggg cattttatag catatgcggt ggaaccgttc tccaatcata 7860 ttgtgacgcg tgcccatgta tggtgtgact tggtgtttat gacacagatg atatttcggg 7920 gagtccttcg tagttgacgg tttcgtaatc ttgacggagt cagagatgta ccttcaccgc 7980 ttcacgcttg gctttatccg ttaccacggc gttattaaat tgggcccgca cccgttgcaa 8040 gttaggacca cagtgtccat tacctgaacg gtcggctgct accagtattt tacattatta 8100 aaatctatgg aaaagctgtc acgctagatt tttttttttt tttttatgga taagaaaaga 8160 ctaataggaa agctttctta ctccactaaa gtctttaata attatttttt caattttcaa 8220 ataaaatcat aatcatccca ctaaattctt tctcatttgt ttttcacttt gtaagatatt 8280 cttccgtaga tttttataag taattcttta agatacatag cataataaat tgtatacttt 8340 tagttacagt ctatataaaa ttagtcattt aaaaaatata gccttaatga tttttttaaa 8400 atgaagttat ttttttctaa tttttctttc ttatttatta tcgaatgatt ttttctacta 8460 atcgtttagg gaagagtttt atttttctaa tgacgagatg tgttaaatga tgcaacttga 8520 aggcgacata ttttttacca ataaaataaa actaaaagat agcttgttga caaaaacaat 8580 aattgatgtt aaagaaaacg taagggcaat gggtatcatc taattgttgg acaagttgtc 8640 ttctaaggct aggtaagctt ttgtttttta ttagctgaaa tatttattta attgttctat 8700 aaataaattt tttaaaatga tttttaatgt ttttaaatat tatttaaaat aattttttta 8760 aaattttaac attttagttt ttaattattt tttatcttac ttaaattaat tataatttat 8820 tattttctac cattttatac tttccaatta attttatcga agcattaata ccatctggta 8880 ttttgtcact gcttcgtatg attgtttatt aaattttagc ttctttcaca ttggagatgc 8940 tgggcggtgt gagttacagc atggtgtgaa gttaaggtgg tttgattatg gaacccgaag 9000 gtgttggtgc aaggtggata aaattgcttg ccaggagata cataaaatga tttacaaaat 9060 gaaataaaga ataaataaat agtcaagttc aaatttaaat caaggattag aattattttt 9120 ccgtacatga tgtaaccgca atcaccccct gctgaatcca gatggccatt ctggccgaat 9180 ttaagtgact agggtcaccc tgctgaatcc agatggccat tctggccgaa tttaagtgac 9240 tagggtcacg tgaccctagt cacttaccgg attctggccg taccgagctc gaattcggcc 9300 caacttttct atacaaagtt gaggtacgag atccgcccat ccggccggcc agatcctgca 9360 ggagatccaa gcttggcgcg ccgcggacct gaattccagc acactggcgg ccgttactag 9420 taacggccgc cagtgtgctg gaattcgccc ttcccaagct ttgctctaga tcaaactcac 9480 atccaaacat aacatggata tcttccttac caatcatact aattattttg ggttaaatat 9540 taatcattat ttttaagata ttaattaaga aattaaaaga ttttttaaaa aaatgtataa 9600 aattatatta ttcatgattt ttcatacatt tgattttgat aataaatata ttttttttaa 9660 tttcttaaaa aatgttgcaa gacacttatt agacatagtc ttgttctgtt tacaaaagca 9720 ttcatcattt aatacattaa aaaatattta atactaacag tagaatcttc ttgtgagtgg 9780 tgtgggagta ggcaacctgg cattgaaacg agagaaagag agtcagaacc agaagacaaa 9840 taaaaagtat gcaacaaaca aatcaaaatc aaagggcaaa ggctggggtt ggctcaattg 9900 gttgctacat tcaattttca actcagtcaa cggttgagat tcactctgac ttccccaatc 9960 taagccgcgg atgcaaacgg ttgaatctaa cccacaatcc aatctcgtta cttaggggct 10020 tttccgtcat taactcaccc ctgccacccg gtttccctat aaattggaac tcaatgctcc 10080 cctctaaact cgtatcgctt cagagttgag accaagacac actcgttcat atatctctct 10140 gctcttctct tctcttctac ctctcaaggt acttttcttc tccctctacc aaatcctaga 10200 ttccgtggtt caatttcgga tcttgcactt ctggtttgct ttgccttgct ttttcctcaa 10260 ctgggtccat ctaggatcca tgtgaaactc tactctttct ttaatatctg cggaatacgc 10320 gtttgacttt cagatctagt cgaaatcatt tcataattgc ctttctttct tttagcttat 10380 gagaaataaa atcacttttt ttttatttca aaataaacct tgggccttgt gctgactgag 10440 atggggtttg gtgattacag aattttagcg aattttgtaa ttgtacttgt ttgtctgtag 10500 ttttgttttg ttttcttgtt tctcatacat tccttaggct tcaattttat tcgagtatag 10560 gtcacaatag gaattcaaac tttgagcagg ggaattaatc ccttccttca aatccagttt 10620 gtttgtatat atgtttaaaa aatgaaactt ttgctttaaa ttctattata acttttttta 10680 tggctgaaat ttttgcatgt gtctttgctc tctgttgtaa atttactgtt taggtactaa 10740 ctctaggctt gttgtgcagt ttttgaagta taacaacaga agttcctatt ccgaagttcc 10800 tattctctag aaagtatagg aacttccacc acacaacaca atggcggcca ccgcttccag 10860 aaccacccga ttctcttctt cctcttcaca ccccaccttc cccaaacgca ttactagatc 10920 caccctccct ctctctcatc aaaccctcac caaacccaac cacgctctca aaatcaaatg 10980 ttccatctcc aaacccccca cggcggcgcc cttcaccaag gaagcgccga ccacggagcc 11040 cttcgtgtca cggttcgcct ccggcgaacc tcgcaagggc gcggacatcc ttgtggaggc 11100 gctggagagg cagggcgtga cgacggtgtt cgcgtacccc ggcggtgcgt cgatggagat 11160 ccaccaggcg ctcacgcgct ccgccgccat ccgcaacgtg ctcccgcgcc acgagcaggg 11220 cggcgtcttc gccgccgaag gctacgcgcg ttcctccggc ctccccggcg tctgcattgc 11280 cacctccggc cccggcgcca ccaacctcgt gagcggcctc gccgacgctt taatggacag 11340 cgtcccagtc gtcgccatca ccggccaggt cgcccgccgg atgatcggca ccgacgcctt 11400 ccaagaaacc ccgatcgtgg aggtgagcag atccatcacg aagcacaact acctcatcct 11460 cgacgtcgac gacatccccc gcgtcgtcgc cgaggctttc ttcgtcgcca cctccggccg 11520 ccccggtccg gtcctcatcg acattcccaa agacgttcag cagcaactcg ccgtgcctaa 11580 ttgggacgag cccgttaacc tccccggtta cctcgccagg ctgcccaggc cccccgccga 11640 ggcccaattg gaacacattg tcagactcat catggaggcc caaaagcccg ttctctacgt 11700 cggcggtggc agtttgaatt ccagtgctga attgaggcgc tttgttgaac tcactggtat 11760 tcccgttgct agcactttaa tgggtcttgg aacttttcct attggtgatg aatattccct 11820 tcagatgctg ggtatgcatg gtactgttta tgctaactat gctgttgaca atagtgattt 11880 gttgcttgcc tttggggtaa ggtttgatga ccgtgttact gggaagcttg aggcttttgc 11940 tagtagggct aagattgttc acattgatat tgattctgcc gagattggga agaacaagca 12000 ggcgcacgtg tcggtttgcg cggatttgaa gttggccttg aagggaatta atatgatttt 12060 ggaggagaaa ggagtggagg gtaagtttga tcttggaggt tggagagaag agattaatgt 12120 gcagaaacac aagtttccat tgggttacaa gacattccag gacgcgattt ctccgcagca 12180 tgctatcgag gttcttgatg agttgactaa tggagatgct attgttagta ctggggttgg 12240 gcagcatcaa atgtgggctg cgcagtttta caagtacaag agaccgaggc agtggttgac 12300 ctcagggggt cttggagcca tgggttttgg attgcctgcg gctattggtg ctgctgttgc 12360 taaccctggg gctgttgtgg ttgacattga tggggatggt agtttcatca tgaatgttca 12420 ggagttggcc actataagag tggagaatct cccagttaag atattgttgt tgaacaatca 12480 gcatttgggt atggtggttc agttggagga taggttctac aagtccaata gagctcacac 12540 ctatcttgga gatccgtcta gcgagagcga gatattccca aacatgctca agtttgctga 12600 tgcttgtggg ataccggcag cgcgagtgac gaagaaggaa gagcttagag cggcaattca 12660 gagaatgttg gacacccctg gcccctacct tcttgatgtc attgtgcccc atcaggagca 12720 tgtgttgccg atgattccca gtaatggatc cttcaaggat gtgataactg agggtgatgg 12780 tagaacgagg tactgattgc ctagaccaaa tgttccttga tgcttgtttt gtacaatata 12840 tataagataa tgctgtccta gttgcaggat ttggcctgtg gtgagcatca tagtctgtag 12900 tagttttggt agcaagacat tttattttcc ttttatttaa cttactacat gcagtagcat 12960 ctatctatct ctgtagtctg atatctcctg ttgtctgtat tgtgccgttg gattttttgc 13020 tgtagtgaga ctgaaaatga tgtgctagta ataatatttc tgttagaaat ctaagtagag 13080 aatctgttga agaagtcaaa agctaatgga atcaggttac atattcaatg tttttctttt 13140 tttagcggtt ggtagacgtg tagattcaac ttctcttgga gctcacctag gcaatcagta 13200 aaatgcatat tcctttttta acttgccatt tatttacttt tagtggaaat tgtgaccaat 13260 ttgttcatgt agaacggatt tggaccattg cgtccacaaa acgtctcttt tgctcgatct 13320 tcacaaagcg ataccgaaat ccagagatag ttttcaaaag tcagaaatgg caaagttata 13380 aatagtaaaa cagaatagat gctgtaatcg acttcaataa caagtggcat cacgtttcta 13440 gttctagacc catcagctga ggtacaaatt aattaataag tgactagggt cacgtgaccc 13500 tagtcactta tgattccgat gacttcgtag gttcctagct caagccgctc gtgtccaagc 13560 gtcacttacg attagctaat gattacggca tctaggaccg actagttctt atgtgcttct 13620 agtctccaaa tgtggttgat agttattttg ctctaagatc aacagtaatg aagtataaat 13680 catcgttgtg gtgtgctact cggttaattg agcattaaca cacacaaaca tgacgaggat 13740 ggtataatct ccaaaaatgt gtactttgtt aggtgggacc ctatagcctt gattaatgtg 13800 ctatgttagg catgcctgga aacgtgtgac gcatatgttt tgtgaacctg ttgatattat 13860 atgtgctttt atattaccat attttattaa aatactaata tttattacta gtaagatata 13920 acattctatc tagcttaaaa actaaccata aatattccat aataactaga tttaccaaac 13980 taatatacta aatatacata ataaatacaa aattaacaag acaataatca atatttatga 14040 gcttaatata tttagacatt atggttggtc gacgataatc atgctaactt ttcgtaattg 14100 cttgattgaa atatgcttag aataatgcct ctttgttcta catggcaaat agggaccatt 14160 atggtgtaac accctgggaa ccacaaacac cccgaaatgc tactaaacta cacaactaac 14220 cttcatatat aaaatttcga cagcatctcc tttgaaaatt tgcatagacg tggaagcaac 14280 agagtataaa cagatctagc tagctaagtt taaacggccg gccatttaca attgaatata 14340 tcctgcca 14348 <210> 113 <211> 14348 <212> DNA <213> Artificial Sequence <220> <223> PHP80734 T-DNA <400> 113 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccgtcg acggatccgt accatcaact ttgtataata aagttgcccg 1320 cggccgccgg tacctctgtt aatgaacgta aggagcttct ggatgacggc tgggacttct 1380 ttgacaaaga gacagagctc taagtgtctt cttttcagta gattatcggt gctagaataa 1440 aagagatatg aagggattct gggttctgct ctatgtagag taaagcttgg atctttgatc 1500 taatctaccg ttagtataaa acatctattg agctctcttt tatttttctt ctcagtgtat 1560 agtttgctta ttgttttttt ggtgtttttt tccacctctt tgtggtactg acataaacaa 1620 agactatcgt tttgtcatag aattttgaga ttttgaagaa aagttggata atttagtata 1680 ctttttaact gactttataa aacgaattag cttcaattag agcttcacaa ttctcggatt 1740 ttactgaatg acaagttaac aatttaagct agaaaaaaat atgctctaag cctggacatg 1800 ttgttagtca tggccagcgt atgtgtacat taggtaaatg aaaacaaaaa caaaaacttt 1860 gaaggtttct gttaagaaag gaactaattt agcaataaga gacatgatat tacaagctca 1920 acactgtcgc tatatctacc agaagccaac aaatcttcca gtgcgtacca ttggagccac 1980 ttttcaggct cattagacaa aagtagcaca tataatgtaa agacgaaacg atgataaaga 2040 gactcacaag gacataattt caatacagaa gaagtttgat gtcattactc attagaaaga 2100 aaggtataac acaggaacgg aaacatagtt gaacaattat tcatcagggg ctttacaagg 2160 ccccaaaaca caaaccacca aagttttaaa tgaaacgaga catgaacttc ttctgattca 2220 taacagagat aagtttccta ggtcccaatc tagttcagtc tcaattccag ggaagcgcat 2280 ggtctgacct cggactgtcc atacttccag atagcgccag atcctccgtt gatgtggtcc 2340 tcaccgtgca ttggaaagag agggagagtc cttctgtgtt ccaagtaagc atcacctccg 2400 cattcctctt cttcttggtg tccctccaag ccgaagagag gtttagccct atcgaagaag 2460 ttgtagggag cggaggagta gtggtgatcc atgtttgccc atccgcctcc aacgttgttg 2520 tagttcatgg agcaatcttg ctccatagag ccgtacacgt gggaagacat gtagccgttg 2580 gaggcgttga caactccaca ttcggttcca gaggaggcat ggttgagatt cccgttgttg 2640 aaagaggggt agggcttgtt gtggtggtag aggtgatggt cctggttcaa cttcacgttg 2700 acggagtttg cgggtctttg cataggaact ccgtggtggt gatgaagcaa aggatggtag 2760 tggtcgttgg cggccatcat aacagaattg ggtgatgagg aaggagtggt catgttagtc 2820 ccgttgaaac gcttcttctg cctctcacga gccttgtggt tctggaacca gtagaagacg 2880 ttcttaccct cgatcttacc gaattgacgc aggcgagcgg tgatcttctg aatctgatca 2940 gcagtagggg agcggatggc attgttgtag tacaactcct tcaggatctt gatctgctca 3000 gtggtaggtg tccatctggt ggaggtctgt ctgcaagtgt accctccgga accggacttg 3060 ttgttgttgt tgttcccgga ctcttgatcg gcctgatggt ggtgatgttg gtgctgagga 3120 ggctccatgg tcttgcttcc caaaaccaca cttcacttgt gtaactgtgt tttgtgtgtt 3180 tttaaatgtg gtgagagata aggtaataag ataaccaaac cttacttcag ttgagccaaa 3240 cctgtctcta acaccccaca acaaaacttt cccatatcag aaaccaaaca aataaaaagc 3300 tttaaactat caaaacatta ttctcattca acccagaaaa aaacaagaga aaaagattcc 3360 ctacaaaacc aaccaaccag aatcttacaa attatttttt agcatgttac atgatcggaa 3420 gaaaagaaaa aattaaagag agaaaaaaat caaagttaat aagttatatg gaataccaga 3480 aggagcaatg tgatgaggag gttgcaagag agtggttttt ttggtgatgt tagttcactt 3540 gagagttcac tcagttgaca aaacacacgc acacaaaagg ggacagagtc cctttctctc 3600 tttctctttc tttttctcct tagtccttta ccttatccta gttttctttt ctacgttcct 3660 ctctctcttc atataatgct catattcatt cattcaattc atagtgtgtt gtaataaatg 3720 ggttaattta atttaattta ataaatttac tgtaaatggt aaatgcgggt tgacagaaac 3780 cgatcagcaa tacttactac gtcggctgtt acgctccacg tggtgctttc ttaatggcca 3840 ttctaaacgt ttcccacacc cttcaccaaa gtcaaacctt tttttcctct cagttcctct 3900 cccacgcgct tcctgcctcc ttacgtcttc caaggaagag agtgggatac acgcgccgtg 3960 ttggatgatg tcaggtttga gttactcata gggtgtgaaa agaaagagtt tggattgaga 4020 tcgatgctgg atgtgatgaa aatgaaaaac agctttataa aaagataagg cttttaatgt 4080 ttctattaat actattatct aattaaaatt agtgattgat ttttatctta gatgaatgtg 4140 aatgatatgt ttaaggtgga ctgtgaagta tttgaagttc aattttttta attttttttt 4200 tctgaattca actgttgttt caatgtgact gaaatatcgt ggaaaattga taggtacttc 4260 taaaagcaac ttcaatgcca acgttagtag aaatttcgtc aaagtaacgc atatattatt 4320 gttgcaatat atgacgttag aagtatatat agagtaaaaa tgagttaaat ttccttgtgg 4380 acaggaccca agacctcatg ggcatatgat atccaatttt cttatacaaa ttggcttaac 4440 ccaattcgta atcttgttaa tcttttcttt aaaaatcgta atcttgataa tctaagtgat 4500 aaatatttac attaaaaaat aaatagatta ttcagatgaa attatattta gtttttgtga 4560 tataaaaaat attactactg tataaaattg ttgtattaac atttgtcaaa aacacaattt 4620 attgtctttc agaattcact aaattacctg agagttattc tctttatttt gaagaatctc 4680 aattactaag tagtacgtac aattatgtga ttttaatatt aaatttatta gaaagagtac 4740 tactatacta attaaataga aataatagta acactttgta ctttttgaga gatggcatgt 4800 ttggtaattg gcaatttttt aatttgggag tgagagaaat attgatttgt aaccgaatgg 4860 agaatggtca taaagtagta gtagggtcaa atagatcaca gccgttaatt tatgaagaag 4920 gagattggag aattggatgg tggaaattaa gggtgggaga tggggtgact tattaaatga 4980 ggtttgacac tgaaacccgt gagatccaag ggtgtcactt tcaggtgggg tccacgccat 5040 cgatccaaag cccctatgga aataaatttg tagacaggga tttttattgc cttggaaatt 5100 taattatgat cattctgcaa caaatttaaa cccgggccat tctggcccac tttgtacaag 5160 aaagctgggt ctagatatct cgagtgcggc cgcgaattcg gtaccggatc cagtcgactg 5220 aattggttcc ggcgccagcc tgcttttttg tacaaacttg aagcttcggc cggggccgca 5280 ttcgcaaaac acacctagac tagatttgtt ttgctaaccc aattcgagct cggtacctcg 5340 cgaatgcatc tagatatcgg atcccggggt taaccttcag cttagaaatg atgtgttttc 5400 tacacttttc tcttcttttt tttcttttca tctcacatga atcaaaaaga gaaagcttaa 5460 ttacattaaa ggttgatgtg atatttactt tctaaatatt tgctagaaga aattataatg 5520 ataacattaa attaagttat agtcatttct atttcggttg tctcccactt tctgctatgc 5580 ttttacaaca acagttcaag atttggaatc gttttctttc tccaccaatc gtagtaatac 5640 ttctttttgt gtttgttgaa attttgcaag attctctctt cctctggtta atacacataa 5700 cttacttggt aagcttggct gcattttctt ttcaagcttt taatcaaaga ctcactagaa 5760 attgcaaagg caaacataaa attatgtgat caaactccta ctgtgagttt ttggttacaa 5820 acaaaagtta aaaacaaaag cttgatattt aaaagataaa ctcaacacat atgcatttga 5880 ctggcgcaca gaagacaatt caagacttgg gaaaacagtg agccatcaaa atcgtcagat 5940 aattcaggga atatattgaa tgtggagatg tggtaatcac actattgcac acaaagagtg 6000 gcaaacaagc ggcgcccttg gtttggtata gtaagagaca attgttttgg actgtttgtg 6060 ttgccatatt cacaaaaata ctattgagct atgtatgtta aacattatgg tcaatgagta 6120 gtagtgaaca ttcaagtttt tttttttttc cacttcaagt ttttcatctt attattattt 6180 ttcgttgtga tgcagtagta cggcccatct tcaccagtag tatatcaaag ccgaaacaga 6240 tgacaaattg tttaacatgc acttggatta ttattgagag ttgaaataca aaggcccgtt 6300 acaagcccaa acgaaggtac ataaaagaaa agcagaaaat gaaaacgaaa tcgataggga 6360 taagtttttc caactgacgg aaacacaaac tacaccaggt gaaatagatc acctcccaag 6420 tgcatgagac acgaaccaac cacttatcat cagatcacaa aagagctcaa agatctagcg 6480 atcgcctaat tcagtttgtg gccaagcttt gatggcaagt cgcaatatct agcgacggcc 6540 acctcgtgct gctccacata agtctccttg tcggcctctt ttatgcgctc gagtctatgg 6600 tccacgtaat acacgccggg cattttcaag tttttggcag gctttttgga gcggtaggtg 6660 gtcttgaagt tacagatgag atggcctccg ccgacaagct tcaaggccat gtcggatcta 6720 ccttcaagac caccgtcggc tgggtaaagc atttcagtat tggcttccca gccgagagtc 6780 ttcttctgca tcacgggacc gtttgatggg aagttcacgc cgcggatctt gacgttgtag 6840 atcaagcagc cgtcttgaag actagtgtct tgggtggcgg tcaagactcc tccgtcttcg 6900 taggtggtca ctctctccca ggtgaatccc tctgggaatg actgtttgaa gaagtctggg 6960 atgccctggg tgtggttgat gaaggttcta ctcccgtaca tgaaggaggt ggctagtatg 7020 tcgaaggcga atgggagtgg acctccttcg accaccttga ttctcatagt ctgggttccc 7080 tcatagggct tgccctcacc ctcagaggta cacttgaagt ggtgattatt cacggtgccc 7140 tccatgtata acttcatgtg catgttctct ttaatgagct cctccccctt ggacaccatg 7200 gctgtagagt caacaatcac aagataaatc agaatcaaaa tccatcacgt aatttcaaga 7260 agcaatttcg tgaaaaaaaa tcctaatcta tggccacaga atcaacaaat aaggtagttg 7320 ctaaaggacc tgagcaatat ctcttgaaat accatacaga atcaacgaat aaggctttta 7380 tcaacggatc tgaacaattt cttgaaaaac gactgaagta tcaacaaata aggctgttgt 7440 ttaacggatc tgaagtagtt catgaaaaaa accttaatct acgactacag aatcaacaat 7500 taaggcttgt tgtaaacgga tctgaaaatc tttatgaaaa aaactctaat tagacctaat 7560 ttacggttat aaaatcatca attaaggacg ttcttgcatt aataaaagct gaatcttctg 7620 attatgaaaa tttataatcg atgaaacgat agaaaacaca atcaaatcaa catcatgctg 7680 atgtagaaca aacaataaag aagaagaaaa aaaaacggaa gaaatctacg aaccttgaaa 7740 ggagaggtag ggaactgcgc aaagagaatg aactaatttg attgcaattg aagtgcgatt 7800 agggtcgagg ggtgtggggg cattttatag catatgcggt ggaaccgttc tccaatcata 7860 ttgtgacgcg tgcccatgta tggtgtgact tggtgtttat gacacagatg atatttcggg 7920 gagtccttcg tagttgacgg tttcgtaatc ttgacggagt cagagatgta ccttcaccgc 7980 ttcacgcttg gctttatccg ttaccacggc gttattaaat tgggcccgca cccgttgcaa 8040 gttaggacca cagtgtccat tacctgaacg gtcggctgct accagtattt tacattatta 8100 aaatctatgg aaaagctgtc acgctagatt tttttttttt tttttatgga taagaaaaga 8160 ctaataggaa agctttctta ctccactaaa gtctttaata attatttttt caattttcaa 8220 ataaaatcat aatcatccca ctaaattctt tctcatttgt ttttcacttt gtaagatatt 8280 cttccgtaga tttttataag taattcttta agatacatag cataataaat tgtatacttt 8340 tagttacagt ctatataaaa ttagtcattt aaaaaatata gccttaatga tttttttaaa 8400 atgaagttat ttttttctaa tttttctttc ttatttatta tcgaatgatt ttttctacta 8460 atcgtttagg gaagagtttt atttttctaa tgacgagatg tgttaaatga tgcaacttga 8520 aggcgacata ttttttacca ataaaataaa actaaaagat agcttgttga caaaaacaat 8580 aattgatgtt aaagaaaacg taagggcaat gggtatcatc taattgttgg acaagttgtc 8640 ttctaaggct aggtaagctt ttgtttttta ttagctgaaa tatttattta attgttctat 8700 aaataaattt tttaaaatga tttttaatgt ttttaaatat tatttaaaat aattttttta 8760 aaattttaac attttagttt ttaattattt tttatcttac ttaaattaat tataatttat 8820 tattttctac cattttatac tttccaatta attttatcga agcattaata ccatctggta 8880 ttttgtcact gcttcgtatg attgtttatt aaattttagc ttctttcaca ttggagatgc 8940 tgggcggtgt gagttacagc atggtgtgaa gttaaggtgg tttgattatg gaacccgaag 9000 gtgttggtgc aaggtggata aaattgcttg ccaggagata cataaaatga tttacaaaat 9060 gaaataaaga ataaataaat agtcaagttc aaatttaaat caaggattag aattattttt 9120 ccgtacatga tgtaaccgca atcaccccct gctgaatcca gatggccatt ctggccgaat 9180 ttaagtgact agggtcaccc tgctgaatcc agatggccat tctggccgaa tttaagtgac 9240 tagggtcacg tgaccctagt cacttaccgg attctggccg taccgagctc gaattcggcc 9300 caacttttct atacaaagtt gaggtacgag atccgcccat ccggccggcc agatcctgca 9360 ggagatccaa gcttggcgcg ccgcggacct gaattccagc acactggcgg ccgttactag 9420 taacggccgc cagtgtgctg gaattcgccc ttcccaagct ttgctctaga tcaaactcac 9480 atccaaacat aacatggata tcttccttac caatcatact aattattttg ggttaaatat 9540 taatcattat ttttaagata ttaattaaga aattaaaaga ttttttaaaa aaatgtataa 9600 aattatatta ttcatgattt ttcatacatt tgattttgat aataaatata ttttttttaa 9660 tttcttaaaa aatgttgcaa gacacttatt agacatagtc ttgttctgtt tacaaaagca 9720 ttcatcattt aatacattaa aaaatattta atactaacag tagaatcttc ttgtgagtgg 9780 tgtgggagta ggcaacctgg cattgaaacg agagaaagag agtcagaacc agaagacaaa 9840 taaaaagtat gcaacaaaca aatcaaaatc aaagggcaaa ggctggggtt ggctcaattg 9900 gttgctacat tcaattttca actcagtcaa cggttgagat tcactctgac ttccccaatc 9960 taagccgcgg atgcaaacgg ttgaatctaa cccacaatcc aatctcgtta cttaggggct 10020 tttccgtcat taactcaccc ctgccacccg gtttccctat aaattggaac tcaatgctcc 10080 cctctaaact cgtatcgctt cagagttgag accaagacac actcgttcat atatctctct 10140 gctcttctct tctcttctac ctctcaaggt acttttcttc tccctctacc aaatcctaga 10200 ttccgtggtt caatttcgga tcttgcactt ctggtttgct ttgccttgct ttttcctcaa 10260 ctgggtccat ctaggatcca tgtgaaactc tactctttct ttaatatctg cggaatacgc 10320 gtttgacttt cagatctagt cgaaatcatt tcataattgc ctttctttct tttagcttat 10380 gagaaataaa atcacttttt ttttatttca aaataaacct tgggccttgt gctgactgag 10440 atggggtttg gtgattacag aattttagcg aattttgtaa ttgtacttgt ttgtctgtag 10500 ttttgttttg ttttcttgtt tctcatacat tccttaggct tcaattttat tcgagtatag 10560 gtcacaatag gaattcaaac tttgagcagg ggaattaatc ccttccttca aatccagttt 10620 gtttgtatat atgtttaaaa aatgaaactt ttgctttaaa ttctattata acttttttta 10680 tggctgaaat ttttgcatgt gtctttgctc tctgttgtaa atttactgtt taggtactaa 10740 ctctaggctt gttgtgcagt ttttgaagta taacaacaga agttcctatt ccgaagttcc 10800 tattctctag aaagtatagg aacttccacc acacaacaca atggcggcca ccgcttccag 10860 aaccacccga ttctcttctt cctcttcaca ccccaccttc cccaaacgca ttactagatc 10920 caccctccct ctctctcatc aaaccctcac caaacccaac cacgctctca aaatcaaatg 10980 ttccatctcc aaacccccca cggcggcgcc cttcaccaag gaagcgccga ccacggagcc 11040 cttcgtgtca cggttcgcct ccggcgaacc tcgcaagggc gcggacatcc ttgtggaggc 11100 gctggagagg cagggcgtga cgacggtgtt cgcgtacccc ggcggtgcgt cgatggagat 11160 ccaccaggcg ctcacgcgct ccgccgccat ccgcaacgtg ctcccgcgcc acgagcaggg 11220 cggcgtcttc gccgccgaag gctacgcgcg ttcctccggc ctccccggcg tctgcattgc 11280 cacctccggc cccggcgcca ccaacctcgt gagcggcctc gccgacgctt taatggacag 11340 cgtcccagtc gtcgccatca ccggccaggt cgcccgccgg atgatcggca ccgacgcctt 11400 ccaagaaacc ccgatcgtgg aggtgagcag atccatcacg aagcacaact acctcatcct 11460 cgacgtcgac gacatccccc gcgtcgtcgc cgaggctttc ttcgtcgcca cctccggccg 11520 ccccggtccg gtcctcatcg acattcccaa agacgttcag cagcaactcg ccgtgcctaa 11580 ttgggacgag cccgttaacc tccccggtta cctcgccagg ctgcccaggc cccccgccga 11640 ggcccaattg gaacacattg tcagactcat catggaggcc caaaagcccg ttctctacgt 11700 cggcggtggc agtttgaatt ccagtgctga attgaggcgc tttgttgaac tcactggtat 11760 tcccgttgct agcactttaa tgggtcttgg aacttttcct attggtgatg aatattccct 11820 tcagatgctg ggtatgcatg gtactgttta tgctaactat gctgttgaca atagtgattt 11880 gttgcttgcc tttggggtaa ggtttgatga ccgtgttact gggaagcttg aggcttttgc 11940 tagtagggct aagattgttc acattgatat tgattctgcc gagattggga agaacaagca 12000 ggcgcacgtg tcggtttgcg cggatttgaa gttggccttg aagggaatta atatgatttt 12060 ggaggagaaa ggagtggagg gtaagtttga tcttggaggt tggagagaag agattaatgt 12120 gcagaaacac aagtttccat tgggttacaa gacattccag gacgcgattt ctccgcagca 12180 tgctatcgag gttcttgatg agttgactaa tggagatgct attgttagta ctggggttgg 12240 gcagcatcaa atgtgggctg cgcagtttta caagtacaag agaccgaggc agtggttgac 12300 ctcagggggt cttggagcca tgggttttgg attgcctgcg gctattggtg ctgctgttgc 12360 taaccctggg gctgttgtgg ttgacattga tggggatggt agtttcatca tgaatgttca 12420 ggagttggcc actataagag tggagaatct cccagttaag atattgttgt tgaacaatca 12480 gcatttgggt atggtggttc agttggagga taggttctac aagtccaata gagctcacac 12540 ctatcttgga gatccgtcta gcgagagcga gatattccca aacatgctca agtttgctga 12600 tgcttgtggg ataccggcag cgcgagtgac gaagaaggaa gagcttagag cggcaattca 12660 gagaatgttg gacacccctg gcccctacct tcttgatgtc attgtgcccc atcaggagca 12720 tgtgttgccg atgattccca gtaatggatc cttcaaggat gtgataactg agggtgatgg 12780 tagaacgagg tactgattgc ctagaccaaa tgttccttga tgcttgtttt gtacaatata 12840 tataagataa tgctgtccta gttgcaggat ttggcctgtg gtgagcatca tagtctgtag 12900 tagttttggt agcaagacat tttattttcc ttttatttaa cttactacat gcagtagcat 12960 ctatctatct ctgtagtctg atatctcctg ttgtctgtat tgtgccgttg gattttttgc 13020 tgtagtgaga ctgaaaatga tgtgctagta ataatatttc tgttagaaat ctaagtagag 13080 aatctgttga agaagtcaaa agctaatgga atcaggttac atattcaatg tttttctttt 13140 tttagcggtt ggtagacgtg tagattcaac ttctcttgga gctcacctag gcaatcagta 13200 aaatgcatat tcctttttta acttgccatt tatttacttt tagtggaaat tgtgaccaat 13260 ttgttcatgt agaacggatt tggaccattg cgtccacaaa acgtctcttt tgctcgatct 13320 tcacaaagcg ataccgaaat ccagagatag ttttcaaaag tcagaaatgg caaagttata 13380 aatagtaaaa cagaatagat gctgtaatcg acttcaataa caagtggcat cacgtttcta 13440 gttctagacc catcagctga ggtacaaatt aattaataag tgactagggt cacgtgaccc 13500 tagtcactta tgattccgat gacttcgtag gttcctagct caagccgctc gtgtccaagc 13560 gtcacttacg attagctaat gattacggca tctaggaccg actagttctt atgtgcttct 13620 agtctccaaa tgtggttgat agttattttg ctctaagatc aacagtaatg aagtataaat 13680 catcgttgtg gtgtgctact cggttaattg agcattaaca cacacaaaca tgacgaggat 13740 ggtataatct ccaaaaatgt gtactttgtt aggtgggacc ctatagcctt gattaatgtg 13800 ctatgttagg catgcctgga aacgtgtgac gcatatgttt tgtgaacctg ttgatattat 13860 atgtgctttt atattaccat attttattaa aatactaata tttattacta gtaagatata 13920 acattctatc tagcttaaaa actaaccata aatattccat aataactaga tttaccaaac 13980 taatatacta aatatacata ataaatacaa aattaacaag acaataatca atatttatga 14040 gcttaatata tttagacatt atggttggtc gacgataatc atgctaactt ttcgtaattg 14100 cttgattgaa atatgcttag aataatgcct ctttgttcta catggcaaat agggaccatt 14160 atggtgtaac accctgggaa ccacaaacac cccgaaatgc tactaaacta cacaactaac 14220 cttcatatat aaaatttcga cagcatctcc tttgaaaatt tgcatagacg tggaagcaac 14280 agagtataaa cagatctagc tagctaagtt taaacggccg gccatttaca attgaatata 14340 tcctgcca 14348 <210> 114 <211> 14348 <212> DNA <213> Artificial Sequence <220> <223> PHP80736 T-DNA <400> 114 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccgtcg acggatccgt accatcaact ttgtataata aagttgcccg 1320 cggccgccgg tacctctgtt aatgaacgta aggagcttct ggatgacggc tgggacttct 1380 ttgacaaaga gacagagctc taagtgtctt cttttcagta gattatcggt gctagaataa 1440 aagagatatg aagggattct gggttctgct ctatgtagag taaagcttgg atctttgatc 1500 taatctaccg ttagtataaa acatctattg agctctcttt tatttttctt ctcagtgtat 1560 agtttgctta ttgttttttt ggtgtttttt tccacctctt tgtggtactg acataaacaa 1620 agactatcgt tttgtcatag aattttgaga ttttgaagaa aagttggata atttagtata 1680 ctttttaact gactttataa aacgaattag cttcaattag agcttcacaa ttctcggatt 1740 ttactgaatg acaagttaac aatttaagct agaaaaaaat atgctctaag cctggacatg 1800 ttgttagtca tggccagcgt atgtgtacat taggtaaatg aaaacaaaaa caaaaacttt 1860 gaaggtttct gttaagaaag gaactaattt agcaataaga gacatgatat tacaagctca 1920 acactgtcgc tatatctacc agaagccaac aaatcttcca gtgcgtacca ttggagccac 1980 ttttcaggct cattagacaa aagtagcaca tataatgtaa agacgaaacg atgataaaga 2040 gactcacaag gacataattt caatacagaa gaagtttgat gtcattactc attagaaaga 2100 aaggtataac acaggaacgg aaacatagtt gaacaattat tcatcagggg ctttacaagg 2160 ccccaaaaca caaaccacca aagttttaaa tgaaacgaga catgaacttc ttctgattca 2220 taacagagat aagtttccta ggtcccaatc tagttcagtc tcaattccag ggaagcgcat 2280 ggtctgacct cggactgtcc atacttccag atagcgccag atcctccgtt gatgtggtcc 2340 tcaccgtgca ttggaaagag agggagagtc cttctgtgtt ccaagtaagc atcacctccg 2400 cattcctctt cttcttggtg tccctccaag ccgaagagag gtttagccct atcgaagaag 2460 ttgtagggag cggaggagta gtggtgatcc atgtttgccc atccgcctcc aacgttgttg 2520 tagttcatgg agcaatcttg ctccatagag ccgtacacgt gggaagacat gtagccgttg 2580 gaggcgttga caactccaca ttcggttcca gaggaggcat ggttgagatt cccgttgttg 2640 aaagaggggt agggcttgtt gtggtggtag aggtgatggt cctggttcaa cttcacgttg 2700 acggagtttg cgggtctttg cataggaact ccgtggtggt gatgaagcaa aggatggtag 2760 tggtcgttgg cggccatcat aacagaattg ggtgatgagg aaggagtggt catgttagtc 2820 ccgttgaaac gcttcttctg cctctcacga gccttgtggt tctggaacca gtagaagacg 2880 ttcttaccct cgatcttacc gaattgacgc aggcgagcgg tgatcttctg aatctgatca 2940 gcagtagggg agcggatggc attgttgtag tacaactcct tcaggatctt gatctgctca 3000 gtggtaggtg tccatctggt ggaggtctgt ctgcaagtgt accctccgga accggacttg 3060 ttgttgttgt tgttcccgga ctcttgatcg gcctgatggt ggtgatgttg gtgctgagga 3120 ggctccatgg ggttattatg tgcactgcaa tacaagtgtc gcaactagtt tcagtgcatg 3180 gaatgaggca ttgcaaataa ccaattatat atgcatgcat gtgcacaagt ggaatatttg 3240 ctccaccgaa gattatgtag ctaaccttcc aacatgtttt gttggcacct ttcagttgtg 3300 ttcacgtgct tcttgacgta gagacaagta gataaaacta ccttcagtta ttaacctttt 3360 attttatcag ccttcagtta ttaatttatt ataggcatgt cacatatggt atctatatat 3420 tttgtacaaa aaaaaaaaca aaaaagtggt tgctggatag caataaaaat tcaatttttt 3480 acacctttga atgattagta ggctgtcact tgaaaaatat gggtactgca aaaaataata 3540 aaaatgtgaa ttgttcaaag tggggaagga atggccttgg tagtgtgaag cagctaaact 3600 aaaagtcttc aaccgactgt taaaaaatga gaaacgtgaa taatagcatc atacatttta 3660 aaaaaacatg tttttagtaa aatataaaag ttttttacac cattgtctaa taaatcatta 3720 tttataataa atttgttaaa ttttaaaatc atcatctata ataaactgta tattttcaat 3780 tgattgataa tgtataaata aggtcagaat tgttgcacaa gaagtgcaag caacaatgct 3840 gcaaaaactt tcaacttaac aatatatatt tattaaactc tttacaattt atataatgtt 3900 cagttaataa taaataatgt gtaaacattt tattggtatt tctcttttag caagcatata 3960 tcatcattgt tttcacgaat tgtcaccttt cgtaggcaat caaccatcat cattttctcc 4020 atgtcggttg tgtttaattg gaggggcata cggtacgtcg tctttttcat ttaataggaa 4080 aaattgggtg tattataata atgcttgtga cgccaacatg ttaatagaat gtatgaatgt 4140 tacctgagct gagtcattgc ttgtgcatgt gtaagcattg ttagacattg caatggagag 4200 tgggatggtt atgagtattg tctctcaatc tcttattcta acttctcaat atatcttcat 4260 attcgtattc gatatcgggt ttaaatttat tttctcattc ctgtacctat ccggtatccc 4320 cgaccctgtc ccatacacga tttaaattaa aaaaatattt ttttttataa aaaaaatatt 4380 aaaagtttga ttttagaaaa agaaagttaa ttgttaaaca tttgttttaa ctacttatat 4440 attaataaat ttattatagc gcatgtgtct acaaaaatag ttaagaaaat aatattaaat 4500 tatgtaaaaa ttctaaaata aaattaacta gtaataaaaa ttttatgcct tttgattaaa 4560 ttatgcaaaa aaatttaaag attttaagtg gcagggcgag ttcgggtttg gggtcgggac 4620 gagtctagta acctcatacc catacccgta tctgactttt gattatcgag aaaaatccaa 4680 acccgaacct ataccaggtc aactcaggta ttacccgtca aagtcgaaac gaattcagac 4740 ggatacccac aagtatgagt tttcttgcca tatctaagca ttgttattac caaaaacaaa 4800 ttaattgtgt taatactggc taacacatta cagacaaaaa aaatattgaa ttattttaac 4860 aagttaatat tctttaaact tccaagatat taacttatga catagtagta acaaaaatat 4920 ttttaaattt tttttctcaa ataagagata ttttccctcg tttatttcag ataaaaaagt 4980 atatttatat tgttcatgag tttaattttc gtgcatggtg ttttgtgaga gttaaaattt 5040 tatactatta accaataaaa aaatctatgt ataactttta aaataattat tttaaaaaat 5100 ttaacaaata gagcatatat aataattcat cccgggccat tctggcccac tttgtacaag 5160 aaagctgggt ctagatatct cgagtgcggc cgcgaattcg gtaccggatc cagtcgactg 5220 aattggttcc ggcgccagcc tgcttttttg tacaaacttg aagcttcggc cggggccgca 5280 ttcgcaaaac acacctagac tagatttgtt ttgctaaccc aattcgagct cggtacctcg 5340 cgaatgcatc tagatatcgg atcccggggt taaccttcag cttagaaatg atgtgttttc 5400 tacacttttc tcttcttttt tttcttttca tctcacatga atcaaaaaga gaaagcttaa 5460 ttacattaaa ggttgatgtg atatttactt tctaaatatt tgctagaaga aattataatg 5520 ataacattaa attaagttat agtcatttct atttcggttg tctcccactt tctgctatgc 5580 ttttacaaca acagttcaag atttggaatc gttttctttc tccaccaatc gtagtaatac 5640 ttctttttgt gtttgttgaa attttgcaag attctctctt cctctggtta atacacataa 5700 cttacttggt aagcttggct gcattttctt ttcaagcttt taatcaaaga ctcactagaa 5760 attgcaaagg caaacataaa attatgtgat caaactccta ctgtgagttt ttggttacaa 5820 acaaaagtta aaaacaaaag cttgatattt aaaagataaa ctcaacacat atgcatttga 5880 ctggcgcaca gaagacaatt caagacttgg gaaaacagtg agccatcaaa atcgtcagat 5940 aattcaggga atatattgaa tgtggagatg tggtaatcac actattgcac acaaagagtg 6000 gcaaacaagc ggcgcccttg gtttggtata gtaagagaca attgttttgg actgtttgtg 6060 ttgccatatt cacaaaaata ctattgagct atgtatgtta aacattatgg tcaatgagta 6120 gtagtgaaca ttcaagtttt tttttttttc cacttcaagt ttttcatctt attattattt 6180 ttcgttgtga tgcagtagta cggcccatct tcaccagtag tatatcaaag ccgaaacaga 6240 tgacaaattg tttaacatgc acttggatta ttattgagag ttgaaataca aaggcccgtt 6300 acaagcccaa acgaaggtac ataaaagaaa agcagaaaat gaaaacgaaa tcgataggga 6360 taagtttttc caactgacgg aaacacaaac tacaccaggt gaaatagatc acctcccaag 6420 tgcatgagac acgaaccaac cacttatcat cagatcacaa aagagctcaa agatctagcg 6480 atcgcctaat tcagtttgtg gccaagcttt gatggcaagt cgcaatatct agcgacggcc 6540 acctcgtgct gctccacata agtctccttg tcggcctctt ttatgcgctc gagtctatgg 6600 tccacgtaat acacgccggg cattttcaag tttttggcag gctttttgga gcggtaggtg 6660 gtcttgaagt tacagatgag atggcctccg ccgacaagct tcaaggccat gtcggatcta 6720 ccttcaagac caccgtcggc tgggtaaagc atttcagtat tggcttccca gccgagagtc 6780 ttcttctgca tcacgggacc gtttgatggg aagttcacgc cgcggatctt gacgttgtag 6840 atcaagcagc cgtcttgaag actagtgtct tgggtggcgg tcaagactcc tccgtcttcg 6900 taggtggtca ctctctccca ggtgaatccc tctgggaatg actgtttgaa gaagtctggg 6960 atgccctggg tgtggttgat gaaggttcta ctcccgtaca tgaaggaggt ggctagtatg 7020 tcgaaggcga atgggagtgg acctccttcg accaccttga ttctcatagt ctgggttccc 7080 tcatagggct tgccctcacc ctcagaggta cacttgaagt ggtgattatt cacggtgccc 7140 tccatgtata acttcatgtg catgttctct ttaatgagct cctccccctt ggacaccatg 7200 gctgtagagt caacaatcac aagataaatc agaatcaaaa tccatcacgt aatttcaaga 7260 agcaatttcg tgaaaaaaaa tcctaatcta tggccacaga atcaacaaat aaggtagttg 7320 ctaaaggacc tgagcaatat ctcttgaaat accatacaga atcaacgaat aaggctttta 7380 tcaacggatc tgaacaattt cttgaaaaac gactgaagta tcaacaaata aggctgttgt 7440 ttaacggatc tgaagtagtt catgaaaaaa accttaatct acgactacag aatcaacaat 7500 taaggcttgt tgtaaacgga tctgaaaatc tttatgaaaa aaactctaat tagacctaat 7560 ttacggttat aaaatcatca attaaggacg ttcttgcatt aataaaagct gaatcttctg 7620 attatgaaaa tttataatcg atgaaacgat agaaaacaca atcaaatcaa catcatgctg 7680 atgtagaaca aacaataaag aagaagaaaa aaaaacggaa gaaatctacg aaccttgaaa 7740 ggagaggtag ggaactgcgc aaagagaatg aactaatttg attgcaattg aagtgcgatt 7800 agggtcgagg ggtgtggggg cattttatag catatgcggt ggaaccgttc tccaatcata 7860 ttgtgacgcg tgcccatgta tggtgtgact tggtgtttat gacacagatg atatttcggg 7920 gagtccttcg tagttgacgg tttcgtaatc ttgacggagt cagagatgta ccttcaccgc 7980 ttcacgcttg gctttatccg ttaccacggc gttattaaat tgggcccgca cccgttgcaa 8040 gttaggacca cagtgtccat tacctgaacg gtcggctgct accagtattt tacattatta 8100 aaatctatgg aaaagctgtc acgctagatt tttttttttt tttttatgga taagaaaaga 8160 ctaataggaa agctttctta ctccactaaa gtctttaata attatttttt caattttcaa 8220 ataaaatcat aatcatccca ctaaattctt tctcatttgt ttttcacttt gtaagatatt 8280 cttccgtaga tttttataag taattcttta agatacatag cataataaat tgtatacttt 8340 tagttacagt ctatataaaa ttagtcattt aaaaaatata gccttaatga tttttttaaa 8400 atgaagttat ttttttctaa tttttctttc ttatttatta tcgaatgatt ttttctacta 8460 atcgtttagg gaagagtttt atttttctaa tgacgagatg tgttaaatga tgcaacttga 8520 aggcgacata ttttttacca ataaaataaa actaaaagat agcttgttga caaaaacaat 8580 aattgatgtt aaagaaaacg taagggcaat gggtatcatc taattgttgg acaagttgtc 8640 ttctaaggct aggtaagctt ttgtttttta ttagctgaaa tatttattta attgttctat 8700 aaataaattt tttaaaatga tttttaatgt ttttaaatat tatttaaaat aattttttta 8760 aaattttaac attttagttt ttaattattt tttatcttac ttaaattaat tataatttat 8820 tattttctac cattttatac tttccaatta attttatcga agcattaata ccatctggta 8880 ttttgtcact gcttcgtatg attgtttatt aaattttagc ttctttcaca ttggagatgc 8940 tgggcggtgt gagttacagc atggtgtgaa gttaaggtgg tttgattatg gaacccgaag 9000 gtgttggtgc aaggtggata aaattgcttg ccaggagata cataaaatga tttacaaaat 9060 gaaataaaga ataaataaat agtcaagttc aaatttaaat caaggattag aattattttt 9120 ccgtacatga tgtaaccgca atcaccccct gctgaatcca gatggccatt ctggccgaat 9180 ttaagtgact agggtcaccc tgctgaatcc agatggccat tctggccgaa tttaagtgac 9240 tagggtcacg tgaccctagt cacttaccgg attctggccg taccgagctc gaattcggcc 9300 caacttttct atacaaagtt gaggtacgag atccgcccat ccggccggcc agatcctgca 9360 ggagatccaa gcttggcgcg ccgcggacct gaattccagc acactggcgg ccgttactag 9420 taacggccgc cagtgtgctg gaattcgccc ttcccaagct ttgctctaga tcaaactcac 9480 atccaaacat aacatggata tcttccttac caatcatact aattattttg ggttaaatat 9540 taatcattat ttttaagata ttaattaaga aattaaaaga ttttttaaaa aaatgtataa 9600 aattatatta ttcatgattt ttcatacatt tgattttgat aataaatata ttttttttaa 9660 tttcttaaaa aatgttgcaa gacacttatt agacatagtc ttgttctgtt tacaaaagca 9720 ttcatcattt aatacattaa aaaatattta atactaacag tagaatcttc ttgtgagtgg 9780 tgtgggagta ggcaacctgg cattgaaacg agagaaagag agtcagaacc agaagacaaa 9840 taaaaagtat gcaacaaaca aatcaaaatc aaagggcaaa ggctggggtt ggctcaattg 9900 gttgctacat tcaattttca actcagtcaa cggttgagat tcactctgac ttccccaatc 9960 taagccgcgg atgcaaacgg ttgaatctaa cccacaatcc aatctcgtta cttaggggct 10020 tttccgtcat taactcaccc ctgccacccg gtttccctat aaattggaac tcaatgctcc 10080 cctctaaact cgtatcgctt cagagttgag accaagacac actcgttcat atatctctct 10140 gctcttctct tctcttctac ctctcaaggt acttttcttc tccctctacc aaatcctaga 10200 ttccgtggtt caatttcgga tcttgcactt ctggtttgct ttgccttgct ttttcctcaa 10260 ctgggtccat ctaggatcca tgtgaaactc tactctttct ttaatatctg cggaatacgc 10320 gtttgacttt cagatctagt cgaaatcatt tcataattgc ctttctttct tttagcttat 10380 gagaaataaa atcacttttt ttttatttca aaataaacct tgggccttgt gctgactgag 10440 atggggtttg gtgattacag aattttagcg aattttgtaa ttgtacttgt ttgtctgtag 10500 ttttgttttg ttttcttgtt tctcatacat tccttaggct tcaattttat tcgagtatag 10560 gtcacaatag gaattcaaac tttgagcagg ggaattaatc ccttccttca aatccagttt 10620 gtttgtatat atgtttaaaa aatgaaactt ttgctttaaa ttctattata acttttttta 10680 tggctgaaat ttttgcatgt gtctttgctc tctgttgtaa atttactgtt taggtactaa 10740 ctctaggctt gttgtgcagt ttttgaagta taacaacaga agttcctatt ccgaagttcc 10800 tattctctag aaagtatagg aacttccacc acacaacaca atggcggcca ccgcttccag 10860 aaccacccga ttctcttctt cctcttcaca ccccaccttc cccaaacgca ttactagatc 10920 caccctccct ctctctcatc aaaccctcac caaacccaac cacgctctca aaatcaaatg 10980 ttccatctcc aaacccccca cggcggcgcc cttcaccaag gaagcgccga ccacggagcc 11040 cttcgtgtca cggttcgcct ccggcgaacc tcgcaagggc gcggacatcc ttgtggaggc 11100 gctggagagg cagggcgtga cgacggtgtt cgcgtacccc ggcggtgcgt cgatggagat 11160 ccaccaggcg ctcacgcgct ccgccgccat ccgcaacgtg ctcccgcgcc acgagcaggg 11220 cggcgtcttc gccgccgaag gctacgcgcg ttcctccggc ctccccggcg tctgcattgc 11280 cacctccggc cccggcgcca ccaacctcgt gagcggcctc gccgacgctt taatggacag 11340 cgtcccagtc gtcgccatca ccggccaggt cgcccgccgg atgatcggca ccgacgcctt 11400 ccaagaaacc ccgatcgtgg aggtgagcag atccatcacg aagcacaact acctcatcct 11460 cgacgtcgac gacatccccc gcgtcgtcgc cgaggctttc ttcgtcgcca cctccggccg 11520 ccccggtccg gtcctcatcg acattcccaa agacgttcag cagcaactcg ccgtgcctaa 11580 ttgggacgag cccgttaacc tccccggtta cctcgccagg ctgcccaggc cccccgccga 11640 ggcccaattg gaacacattg tcagactcat catggaggcc caaaagcccg ttctctacgt 11700 cggcggtggc agtttgaatt ccagtgctga attgaggcgc tttgttgaac tcactggtat 11760 tcccgttgct agcactttaa tgggtcttgg aacttttcct attggtgatg aatattccct 11820 tcagatgctg ggtatgcatg gtactgttta tgctaactat gctgttgaca atagtgattt 11880 gttgcttgcc tttggggtaa ggtttgatga ccgtgttact gggaagcttg aggcttttgc 11940 tagtagggct aagattgttc acattgatat tgattctgcc gagattggga agaacaagca 12000 ggcgcacgtg tcggtttgcg cggatttgaa gttggccttg aagggaatta atatgatttt 12060 ggaggagaaa ggagtggagg gtaagtttga tcttggaggt tggagagaag agattaatgt 12120 gcagaaacac aagtttccat tgggttacaa gacattccag gacgcgattt ctccgcagca 12180 tgctatcgag gttcttgatg agttgactaa tggagatgct attgttagta ctggggttgg 12240 gcagcatcaa atgtgggctg cgcagtttta caagtacaag agaccgaggc agtggttgac 12300 ctcagggggt cttggagcca tgggttttgg attgcctgcg gctattggtg ctgctgttgc 12360 taaccctggg gctgttgtgg ttgacattga tggggatggt agtttcatca tgaatgttca 12420 ggagttggcc actataagag tggagaatct cccagttaag atattgttgt tgaacaatca 12480 gcatttgggt atggtggttc agttggagga taggttctac aagtccaata gagctcacac 12540 ctatcttgga gatccgtcta gcgagagcga gatattccca aacatgctca agtttgctga 12600 tgcttgtggg ataccggcag cgcgagtgac gaagaaggaa gagcttagag cggcaattca 12660 gagaatgttg gacacccctg gcccctacct tcttgatgtc attgtgcccc atcaggagca 12720 tgtgttgccg atgattccca gtaatggatc cttcaaggat gtgataactg agggtgatgg 12780 tagaacgagg tactgattgc ctagaccaaa tgttccttga tgcttgtttt gtacaatata 12840 tataagataa tgctgtccta gttgcaggat ttggcctgtg gtgagcatca tagtctgtag 12900 tagttttggt agcaagacat tttattttcc ttttatttaa cttactacat gcagtagcat 12960 ctatctatct ctgtagtctg atatctcctg ttgtctgtat tgtgccgttg gattttttgc 13020 tgtagtgaga ctgaaaatga tgtgctagta ataatatttc tgttagaaat ctaagtagag 13080 aatctgttga agaagtcaaa agctaatgga atcaggttac atattcaatg tttttctttt 13140 tttagcggtt ggtagacgtg tagattcaac ttctcttgga gctcacctag gcaatcagta 13200 aaatgcatat tcctttttta acttgccatt tatttacttt tagtggaaat tgtgaccaat 13260 ttgttcatgt agaacggatt tggaccattg cgtccacaaa acgtctcttt tgctcgatct 13320 tcacaaagcg ataccgaaat ccagagatag ttttcaaaag tcagaaatgg caaagttata 13380 aatagtaaaa cagaatagat gctgtaatcg acttcaataa caagtggcat cacgtttcta 13440 gttctagacc catcagctga ggtacaaatt aattaataag tgactagggt cacgtgaccc 13500 tagtcactta tgattccgat gacttcgtag gttcctagct caagccgctc gtgtccaagc 13560 gtcacttacg attagctaat gattacggca tctaggaccg actagttctt atgtgcttct 13620 agtctccaaa tgtggttgat agttattttg ctctaagatc aacagtaatg aagtataaat 13680 catcgttgtg gtgtgctact cggttaattg agcattaaca cacacaaaca tgacgaggat 13740 ggtataatct ccaaaaatgt gtactttgtt aggtgggacc ctatagcctt gattaatgtg 13800 ctatgttagg catgcctgga aacgtgtgac gcatatgttt tgtgaacctg ttgatattat 13860 atgtgctttt atattaccat attttattaa aatactaata tttattacta gtaagatata 13920 acattctatc tagcttaaaa actaaccata aatattccat aataactaga tttaccaaac 13980 taatatacta aatatacata ataaatacaa aattaacaag acaataatca atatttatga 14040 gcttaatata tttagacatt atggttggtc gacgataatc atgctaactt ttcgtaattg 14100 cttgattgaa atatgcttag aataatgcct ctttgttcta catggcaaat agggaccatt 14160 atggtgtaac accctgggaa ccacaaacac cccgaaatgc tactaaacta cacaactaac 14220 cttcatatat aaaatttcga cagcatctcc tttgaaaatt tgcatagacg tggaagcaac 14280 agagtataaa cagatctagc tagctaagtt taaacggccg gccatttaca attgaatata 14340 tcctgcca 14348 <210> 115 <211> 14157 <212> DNA <213> Artificial Sequence <220> <223> PHP81060 T-DNA <400> 115 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccgtcg acggatccgt accatcaact ttgtataata aagttgcccg 1320 cggccgccgg tacctctgtt aatgaacgta aggagcttct ggatgacggc tgggacttct 1380 ttgacaaaga gacagagctc taagtgtctt cttttcagta gattatcggt gctagaataa 1440 aagagatatg aagggattct gggttctgct ctatgtagag taaagcttgg atctttgatc 1500 taatctaccg ttagtataaa acatctattg agctctcttt tatttttctt ctcagtgtat 1560 agtttgctta ttgttttttt ggtgtttttt tccacctctt tgtggtactg acataaacaa 1620 agactatcgt tttgtcatag aattttgaga ttttgaagaa aagttggata atttagtata 1680 ctttttaact gactttataa aacgaattag cttcaattag agcttcacaa ttctcggatt 1740 ttactgaatg acaagttaac aatttaagct agaaaaaaat atgctctaag cctggacatg 1800 ttgttagtca tggccagcgt atgtgtacat taggtaaatg aaaacaaaaa caaaaacttt 1860 gaaggtttct gttaagaaag gaactaattt agcaataaga gacatgatat tacaagctca 1920 acactgtcgc tatatctacc agaagccaac aaatcttcca gtgcgtacca ttggagccac 1980 ttttcaggct cattagacaa aagtagcaca tataatgtaa agacgaaacg atgataaaga 2040 gactcacaag gacataattt caatacagaa gaagtttgat gtcattactc attagaaaga 2100 aaggtataac acaggaacgg aaacatagtt gaacaattat tcatcagggg ctttacaagg 2160 ccccaaaaca caaaccacca aagttttaaa tgaaacgaga catgaacttc ttctgattca 2220 taacagagat aagtttccta ggtcccaatc tagttcagtc tcaattccag ggaagcgcat 2280 ggtctgacct cggactgtcc atacttccag atagcgccag atcctccgtt gatgtggtcc 2340 tcaccgtgca ttggaaagag agggagagtc cttctgtgtt ccaagtaagc atcacctccg 2400 cattcctctt cttcttggtg tccctccaag ccgaagagag gtttagccct atcgaagaag 2460 ttgtagggag cggaggagta gtggtgatcc atgtttgccc atccgcctcc aacgttgttg 2520 tagttcatgg agcaatcttg ctccatagag ccgtacacgt gggaagacat gtagccgttg 2580 gaggcgttga caactccaca ttcggttcca gaggaggcat ggttgagatt cccgttgttg 2640 aaagaggggt agggcttgtt gtggtggtag aggtgatggt cctggttcaa cttcacgttg 2700 acggagtttg cgggtctttg cataggaact ccgtggtggt gatgaagcaa aggatggtag 2760 tggtcgttgg cggccatcat aacagaattg ggtgatgagg aaggagtggt catgttagtc 2820 ccgttgaaac gcttcttctg cctctcacga gccttgtggt tctggaacca gtagaagacg 2880 ttcttaccct cgatcttacc gaattgacgc aggcgagcgg tgatcttctg aatctgatca 2940 gcagtagggg agcggatggc attgttgtag tacaactcct tcaggatctt gatctgctca 3000 gtggtaggtg tccatctggt ggaggtctgt ctgcaagtgt accctccgga accggacttg 3060 ttgttgttgt tgttcccgga ctcttgatcg gcctgatggt ggtgatgttg gtgctgagga 3120 ggctccatgg gttttcactc tctcacacac ttcaaaggat aaaatgaaac ttactttata 3180 gacagttttt taatcctccc tcaggagaga agcttcttaa ataataacat taatctttca 3240 gttacttaat ctaacaaatc gtttcgttcg agtattgtaa attgaaggtg agcacgtgat 3300 ggtgttaggt ggtgtgtatt gttaccaacg actcaacatc aacaagtctt gggggtgaac 3360 aaaaaagcct ttcacctttc attaatatcc tacacactat tactattttc tatcgataag 3420 tatctgtgtt ttaatttaaa atttataaat atatattaat tttattttat aaaataaaat 3480 atttatttta aattttctta agattttatt ttaatgagtc aactggtcta ttacgtttgt 3540 tttaataact ataggttgaa tacaatgatc atatttttgt ttgctgcaca aggtgatcta 3600 tctctctctc tctctctata tatatatata tttcttgttc tctaaaaaag aacaacacaa 3660 gcacataact cctttctccc aaatatcttc tttttctttt cctataaaaa cttatttttt 3720 agagcaaaag cattagtgtt cacaagtttc gggattcttt tgctgcacac gtcggtcgaa 3780 accaactggt tgtcgtatct taggagaggg atcattttgc tacacacggt caaaatcaac 3840 tagtgcttca acccaggcta aataattatt tttcttattt attttttcct tatatttatt 3900 gtatgatata gtcatgtggt gttaattaaa attattttgc tattaatatt tttttattta 3960 attcaagact ttgcattctc ttctcattta tttcttttct tttattttaa tttttttcaa 4020 cattttcttc acaattaaac atgtcagact gaattaaaat tttgcacctc atctacaccg 4080 tctatctaac attcaatcgc acaacagaat cacgtatgaa taggaaaata aatggagata 4140 atctagaaat atcattatta ttattgtcta agagctaatt acaaaagaga agaaattggc 4200 cacattaaaa ttaacttctg ggaaggtcca tttaatatcc aaaaattatt cgataacaaa 4260 agtaggacaa gtgagaaatg ttgaagtaaa caaaaaaatt tagatagagt catatctaaa 4320 tgttggctaa gtaagcgtga gtaattatga acccgtgaac catccacatg ggagtggtga 4380 aggaaggacc acaatatatg agattttcct gcccttgaaa gaagaagtag aaaaaaaaga 4440 agaaatatac acttaaaaaa atccttttgt aatgaatcat ttattaagaa aaataaataa 4500 acttaacaca aacccgaaaa agatataata ataacctcca gatcatctac cattatacat 4560 acaaataata aattaaatga cttttcaatg gtatttgtta ctcaaaaaga aaaagaccaa 4620 taactattgg tacacctggt gacccaacta attagtcaaa tgaggattat ttcctatgaa 4680 tatcatgatg aagaagaaaa aaagacatga agagtcattt aatttatgta tgtttactat 4740 tccatgacaa taatgttcaa ttaatccttt tttttattac caccatgtct tatttcatgg 4800 acaagctact ccggtcaaag aaattttggg taatggttat tctctgaaat aataaataaa 4860 ctgttgcatc gtagtcctcc acttccaaag atatatgtga acctcaaatg aaattatgaa 4920 gattttcgtg aaaggttctt tctctttttc tagcaagaat aaataaaaaa agtaacaatt 4980 ttacaaaatt aatcttgtat taaatcttgt ttgttatgca aattgctaat gttagaagta 5040 taaatagaaa aaacaattaa aattaatttt tacttcgagc acacacttat tatgacatga 5100 attctataac aagtgcaaga acatcgaagt cccgggccat tctggcccac tttgtacaag 5160 aaagctgggt gcccggggtt aaccttcagc ttagaaatga tgtgttttct acacttttct 5220 cttctttttt ttcttttcat ctcacatgaa tcaaaaagag aaagcttaat tacattaaag 5280 gttgatgtga tatttacttt ctaaatattt gctagaagaa attataatga taacattaaa 5340 ttaagttata gtcatttcta tttcggttgt ctcccacttt ctgctatgct tttacaacaa 5400 cagttcaaga tttggaatcg ttttctttct ccaccaatcg tagtaatact tctttttgtg 5460 tttgttgaaa ttttgcaaga ttctctcttc ctctggttaa tacacataac ttacttggta 5520 agcttggctg cattttcttt tcaagctttt aatcaaagac tcactagaaa ttgcaaaggc 5580 aaacataaaa ttatgtgatc aaactcctac tgtgagtttt tggttacaaa caaaagttaa 5640 aaacaaaagc ttgatattta aaagataaac tcaacacata tgcatttgac tggcgcacag 5700 aagacaattc aagacttggg aaaacagtga gccatcaaaa tcgtcagata attcagggaa 5760 tatattgaat gtggagatgt ggtaatcaca ctattgcaca caaagagtgg caaacaagcg 5820 gcgcccttgg tttggtatag taagagacaa ttgttttgga ctgtttgtgt tgccatattc 5880 acaaaaatac tattgagcta tgtatgttaa acattatggt caatgagtag tagtgaacat 5940 tcaagttttt ttttttttcc acttcaagtt tttcatctta ttattatttt tcgttgtgat 6000 gcagtagtac ggcccatctt caccagtagt atatcaaagc cgaaacagat gacaaattgt 6060 ttaacatgca cttggattat tattgagagt tgaaatacaa aggcccgtta caagcccaaa 6120 cgaaggtaca taaaagaaaa gcagaaaatg aaaacgaaat cgatagggat aagtttttcc 6180 aactgacgga aacacaaact acaccaggtg aaatagatca cctcccaagt gcatgagaca 6240 cgaaccaacc acttatcatc agatcacaaa agagctcaaa gatctagcga tcgcctaatt 6300 cagtttgtgg ccaagctttg atggcaagtc gcaatatcta gcgacggcca cctcgtgctg 6360 ctccacataa gtctccttgt cggcctcttt tatgcgctcg agtctatggt ccacgtaata 6420 cacgccgggc attttcaagt ttttggcagg ctttttggag cggtaggtgg tcttgaagtt 6480 acagatgaga tggcctccgc cgacaagctt caaggccatg tcggatctac cttcaagacc 6540 accgtcggct gggtaaagca tttcagtatt ggcttcccag ccgagagtct tcttctgcat 6600 cacgggaccg tttgatggga agttcacgcc gcggatcttg acgttgtaga tcaagcagcc 6660 gtcttgaaga ctagtgtctt gggtggcggt caagactcct ccgtcttcgt aggtggtcac 6720 tctctcccag gtgaatccct ctgggaatga ctgtttgaag aagtctggga tgccctgggt 6780 gtggttgatg aaggttctac tcccgtacat gaaggaggtg gctagtatgt cgaaggcgaa 6840 tgggagtgga cctccttcga ccaccttgat tctcatagtc tgggttccct catagggctt 6900 gccctcaccc tcagaggtac acttgaagtg gtgattattc acggtgccct ccatgtataa 6960 cttcatgtgc atgttctctt taatgagctc ctcccccttg gacaccatgg ctgtagagtc 7020 aacaatcaca agataaatca gaatcaaaat ccatcacgta atttcaagaa gcaatttcgt 7080 gaaaaaaaat cctaatctat ggccacagaa tcaacaaata aggtagttgc taaaggacct 7140 gagcaatatc tcttgaaata ccatacagaa tcaacgaata aggcttttat caacggatct 7200 gaacaatttc ttgaaaaacg actgaagtat caacaaataa ggctgttgtt taacggatct 7260 gaagtagttc atgaaaaaaa ccttaatcta cgactacaga atcaacaatt aaggcttgtt 7320 gtaaacggat ctgaaaatct ttatgaaaaa aactctaatt agacctaatt tacggttata 7380 aaatcatcaa ttaaggacgt tcttgcatta ataaaagctg aatcttctga ttatgaaaat 7440 ttataatcga tgaaacgata gaaaacacaa tcaaatcaac atcatgctga tgtagaacaa 7500 acaataaaga agaagaaaaa aaaacggaag aaatctacga accttgaaag gagaggtagg 7560 gaactgcgca aagagaatga actaatttga ttgcaattga agtgcgatta gggtcgaggg 7620 gtgtgggggc attttatagc atatgcggtg gaaccgttct ccaatcatat tgtgacgcgt 7680 gcccatgtat ggtgtgactt ggtgtttatg acacagatga tatttcgggg agtccttcgt 7740 agttgacggt ttcgtaatct tgacggagtc agagatgtac cttcaccgct tcacgcttgg 7800 ctttatccgt taccacggcg ttattaaatt gggcccgcac ccgttgcaag ttaggaccac 7860 agtgtccatt acctgaacgg tcggctgcta ccagtatttt acattattaa aatctatgga 7920 aaagctgtca cgctagattt tttttttttt ttttatggat aagaaaagac taataggaaa 7980 gctttcttac tccactaaag tctttaataa ttattttttc aattttcaaa taaaatcata 8040 atcatcccac taaattcttt ctcatttgtt tttcactttg taagatattc ttccgtagat 8100 ttttataagt aattctttaa gatacatagc ataataaatt gtatactttt agttacagtc 8160 tatataaaat tagtcattta aaaaatatag ccttaatgat ttttttaaaa tgaagttatt 8220 tttttctaat ttttctttct tatttattat cgaatgattt tttctactaa tcgtttaggg 8280 aagagtttta tttttctaat gacgagatgt gttaaatgat gcaacttgaa ggcgacatat 8340 tttttaccaa taaaataaaa ctaaaagata gcttgttgac aaaaacaata attgatgtta 8400 aagaaaacgt aagggcaatg ggtatcatct aattgttgga caagttgtct tctaaggcta 8460 ggtaagcttt tgttttttat tagctgaaat atttatttaa ttgttctata aataaatttt 8520 ttaaaatgat ttttaatgtt tttaaatatt atttaaaata atttttttaa aattttaaca 8580 ttttagtttt taattatttt ttatcttact taaattaatt ataatttatt attttctacc 8640 attttatact ttccaattaa ttttatcgaa gcattaatac catctggtat tttgtcactg 8700 cttcgtatga ttgtttatta aattttagct tctttcacat tggagatgct gggcggtgtg 8760 agttacagca tggtgtgaag ttaaggtggt ttgattatgg aacccgaagg tgttggtgca 8820 aggtggataa aattgcttgc caggagatac ataaaatgat ttacaaaatg aaataaagaa 8880 taaataaata gtcaagttca aatttaaatc aaggattaga attatttttc cgtacatgat 8940 gtaaccgcaa tcaccccctg ctgaatccag atggccattc tggccgaatt taagtgacta 9000 gggtcaccct gctgaatcca gatggccatt ctggccgaat ttaagtgact agggtcacgt 9060 gaccctagtc acttaccgga ttctggccgt accgagctcg aattcggccc aacttttcta 9120 tacaaagttg aggtacgaga tccgcccatc cggccggcca gatcctgcag gagatccaag 9180 cttggcgcgc cgcggacctg aattccagca cactggcggc cgttactagt aacggccgcc 9240 agtgtgctgg aattcgccct tcccaagctt tgctctagat caaactcaca tccaaacata 9300 acatggatat cttccttacc aatcatacta attattttgg gttaaatatt aatcattatt 9360 tttaagatat taattaagaa attaaaagat tttttaaaaa aatgtataaa attatattat 9420 tcatgatttt tcatacattt gattttgata ataaatatat tttttttaat ttcttaaaaa 9480 atgttgcaag acacttatta gacatagtct tgttctgttt acaaaagcat tcatcattta 9540 atacattaaa aaatatttaa tactaacagt agaatcttct tgtgagtggt gtgggagtag 9600 gcaacctggc attgaaacga gagaaagaga gtcagaacca gaagacaaat aaaaagtatg 9660 caacaaacaa atcaaaatca aagggcaaag gctggggttg gctcaattgg ttgctacatt 9720 caattttcaa ctcagtcaac ggttgagatt cactctgact tccccaatct aagccgcgga 9780 tgcaaacggt tgaatctaac ccacaatcca atctcgttac ttaggggctt ttccgtcatt 9840 aactcacccc tgccacccgg tttccctata aattggaact caatgctccc ctctaaactc 9900 gtatcgcttc agagttgaga ccaagacaca ctcgttcata tatctctctg ctcttctctt 9960 ctcttctacc tctcaaggta cttttcttct ccctctacca aatcctagat tccgtggttc 10020 aatttcggat cttgcacttc tggtttgctt tgccttgctt tttcctcaac tgggtccatc 10080 taggatccat gtgaaactct actctttctt taatatctgc ggaatacgcg tttgactttc 10140 agatctagtc gaaatcattt cataattgcc tttctttctt ttagcttatg agaaataaaa 10200 tcactttttt tttatttcaa aataaacctt gggccttgtg ctgactgaga tggggtttgg 10260 tgattacaga attttagcga attttgtaat tgtacttgtt tgtctgtagt tttgttttgt 10320 tttcttgttt ctcatacatt ccttaggctt caattttatt cgagtatagg tcacaatagg 10380 aattcaaact ttgagcaggg gaattaatcc cttccttcaa atccagtttg tttgtatata 10440 tgtttaaaaa atgaaacttt tgctttaaat tctattataa ctttttttat ggctgaaatt 10500 tttgcatgtg tctttgctct ctgttgtaaa tttactgttt aggtactaac tctaggcttg 10560 ttgtgcagtt tttgaagtat aacaacagaa gttcctattc cgaagttcct attctctaga 10620 aagtatagga acttccacca cacaacacaa tggcggccac cgcttccaga accacccgat 10680 tctcttcttc ctcttcacac cccaccttcc ccaaacgcat tactagatcc accctccctc 10740 tctctcatca aaccctcacc aaacccaacc acgctctcaa aatcaaatgt tccatctcca 10800 aaccccccac ggcggcgccc ttcaccaagg aagcgccgac cacggagccc ttcgtgtcac 10860 ggttcgcctc cggcgaacct cgcaagggcg cggacatcct tgtggaggcg ctggagaggc 10920 agggcgtgac gacggtgttc gcgtaccccg gcggtgcgtc gatggagatc caccaggcgc 10980 tcacgcgctc cgccgccatc cgcaacgtgc tcccgcgcca cgagcagggc ggcgtcttcg 11040 ccgccgaagg ctacgcgcgt tcctccggcc tccccggcgt ctgcattgcc acctccggcc 11100 ccggcgccac caacctcgtg agcggcctcg ccgacgcttt aatggacagc gtcccagtcg 11160 tcgccatcac cggccaggtc gcccgccgga tgatcggcac cgacgccttc caagaaaccc 11220 cgatcgtgga ggtgagcaga tccatcacga agcacaacta cctcatcctc gacgtcgacg 11280 acatcccccg cgtcgtcgcc gaggctttct tcgtcgccac ctccggccgc cccggtccgg 11340 tcctcatcga cattcccaaa gacgttcagc agcaactcgc cgtgcctaat tgggacgagc 11400 ccgttaacct ccccggttac ctcgccaggc tgcccaggcc ccccgccgag gcccaattgg 11460 aacacattgt cagactcatc atggaggccc aaaagcccgt tctctacgtc ggcggtggca 11520 gtttgaattc cagtgctgaa ttgaggcgct ttgttgaact cactggtatt cccgttgcta 11580 gcactttaat gggtcttgga acttttccta ttggtgatga atattccctt cagatgctgg 11640 gtatgcatgg tactgtttat gctaactatg ctgttgacaa tagtgatttg ttgcttgcct 11700 ttggggtaag gtttgatgac cgtgttactg ggaagcttga ggcttttgct agtagggcta 11760 agattgttca cattgatatt gattctgccg agattgggaa gaacaagcag gcgcacgtgt 11820 cggtttgcgc ggatttgaag ttggccttga agggaattaa tatgattttg gaggagaaag 11880 gagtggaggg taagtttgat cttggaggtt ggagagaaga gattaatgtg cagaaacaca 11940 agtttccatt gggttacaag acattccagg acgcgatttc tccgcagcat gctatcgagg 12000 ttcttgatga gttgactaat ggagatgcta ttgttagtac tggggttggg cagcatcaaa 12060 tgtgggctgc gcagttttac aagtacaaga gaccgaggca gtggttgacc tcagggggtc 12120 ttggagccat gggttttgga ttgcctgcgg ctattggtgc tgctgttgct aaccctgggg 12180 ctgttgtggt tgacattgat ggggatggta gtttcatcat gaatgttcag gagttggcca 12240 ctataagagt ggagaatctc ccagttaaga tattgttgtt gaacaatcag catttgggta 12300 tggtggttca gttggaggat aggttctaca agtccaatag agctcacacc tatcttggag 12360 atccgtctag cgagagcgag atattcccaa acatgctcaa gtttgctgat gcttgtggga 12420 taccggcagc gcgagtgacg aagaaggaag agcttagagc ggcaattcag agaatgttgg 12480 acacccctgg cccctacctt cttgatgtca ttgtgcccca tcaggagcat gtgttgccga 12540 tgattcccag taatggatcc ttcaaggatg tgataactga gggtgatggt agaacgaggt 12600 actgattgcc tagaccaaat gttccttgat gcttgttttg tacaatatat ataagataat 12660 gctgtcctag ttgcaggatt tggcctgtgg tgagcatcat agtctgtagt agttttggta 12720 gcaagacatt ttattttcct tttatttaac ttactacatg cagtagcatc tatctatctc 12780 tgtagtctga tatctcctgt tgtctgtatt gtgccgttgg attttttgct gtagtgagac 12840 tgaaaatgat gtgctagtaa taatatttct gttagaaatc taagtagaga atctgttgaa 12900 gaagtcaaaa gctaatggaa tcaggttaca tattcaatgt ttttcttttt ttagcggttg 12960 gtagacgtgt agattcaact tctcttggag ctcacctagg caatcagtaa aatgcatatt 13020 ccttttttaa cttgccattt atttactttt agtggaaatt gtgaccaatt tgttcatgta 13080 gaacggattt ggaccattgc gtccacaaaa cgtctctttt gctcgatctt cacaaagcga 13140 taccgaaatc cagagatagt tttcaaaagt cagaaatggc aaagttataa atagtaaaac 13200 agaatagatg ctgtaatcga cttcaataac aagtggcatc acgtttctag ttctagaccc 13260 atcagctgag gtacaaatta attaataagt gactagggtc acgtgaccct agtcacttat 13320 gattccgatg acttcgtagg ttcctagctc aagccgctcg tgtccaagcg tcacttacga 13380 ttagctaatg attacggcat ctaggaccga ctagttctta tgtgcttcta gtctccaaat 13440 gtggttgata gttattttgc tctaagatca acagtaatga agtataaatc atcgttgtgg 13500 tgtgctactc ggttaattga gcattaacac acacaaacat gacgaggatg gtataatctc 13560 caaaaatgtg tactttgtta ggtgggaccc tatagccttg attaatgtgc tatgttaggc 13620 atgcctggaa acgtgtgacg catatgtttt gtgaacctgt tgatattata tgtgctttta 13680 tattaccata ttttattaaa atactaatat ttattactag taagatataa cattctatct 13740 agcttaaaaa ctaaccataa atattccata ataactagat ttaccaaact aatatactaa 13800 atatacataa taaatacaaa attaacaaga caataatcaa tatttatgag cttaatatat 13860 ttagacatta tggttggtcg acgataatca tgctaacttt tcgtaattgc ttgattgaaa 13920 tatgcttaga ataatgcctc tttgttctac atggcaaata gggaccatta tggtgtaaca 13980 ccctgggaac cacaaacacc ccgaaatgct actaaactac acaactaacc ttcatatata 14040 aaatttcgac agcatctcct ttgaaaattt gcatagacgt ggaagcaaca gagtataaac 14100 agatctagct agctaagttt aaacggccgg ccatttacaa ttgaatatat cctgcca 14157 <210> 116 <211> 15357 <212> DNA <213> Artificial Sequence <220> <223> PHP81343 T-DNA <400> 116 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccgtcg acggatccgt accatcaact ttgtataata aagttgcccg 1320 cggccgccgg tacctctgtt aatgaacgta aggagcttct ggatgacggc tgggacttct 1380 ttgacaaaga gacagagctc taagtgtctt cttttcagta gattatcggt gctagaataa 1440 aagagatatg aagggattct gggttctgct ctatgtagag taaagcttgg atctttgatc 1500 taatctaccg ttagtataaa acatctattg agctctcttt tatttttctt ctcagtgtat 1560 agtttgctta ttgttttttt ggtgtttttt tccacctctt tgtggtactg acataaacaa 1620 agactatcgt tttgtcatag aattttgaga ttttgaagaa aagttggata atttagtata 1680 ctttttaact gactttataa aacgaattag cttcaattag agcttcacaa ttctcggatt 1740 ttactgaatg acaagttaac aatttaagct agaaaaaaat atgctctaag cctggacatg 1800 ttgttagtca tggccagcgt atgtgtacat taggtaaatg aaaacaaaaa caaaaacttt 1860 gaaggtttct gttaagaaag gaactaattt agcaataaga gacatgatat tacaagctca 1920 acactgtcgc tatatctacc agaagccaac aaatcttcca gtgcgtacca ttggagccac 1980 ttttcaggct cattagacaa aagtagcaca tataatgtaa agacgaaacg atgataaaga 2040 gactcacaag gacataattt caatacagaa gaagtttgat gtcattactc attagaaaga 2100 aaggtataac acaggaacgg aaacatagtt gaacaattat tcatcagggg ctttacaagg 2160 ccccaaaaca caaaccacca aagttttaaa tgaaacgaga catgaacttc ttctgattca 2220 taacagagat aagtttccta ggtcccaatc tagttcagtc tcaattccag ggaagcgcat 2280 ggtctgacct cggactgtcc atacttccag atagcgccag atcctccgtt gatgtggtcc 2340 tcaccgtgca ttggaaagag agggagagtc cttctgtgtt ccaagtaagc atcacctccg 2400 cattcctctt cttcttggtg tccctccaag ccgaagagag gtttagccct atcgaagaag 2460 ttgtagggag cggaggagta gtggtgatcc atgtttgccc atccgcctcc aacgttgttg 2520 tagttcatgg agcaatcttg ctccatagag ccgtacacgt gggaagacat gtagccgttg 2580 gaggcgttga caactccaca ttcggttcca gaggaggcat ggttgagatt cccgttgttg 2640 aaagaggggt agggcttgtt gtggtggtag aggtgatggt cctggttcaa cttcacgttg 2700 acggagtttg cgggtctttg cataggaact ccgtggtggt gatgaagcaa aggatggtag 2760 tggtcgttgg cggccatcat aacagaattg ggtgatgagg aaggagtggt catgttagtc 2820 ccgttgaaac gcttcttctg cctctcacga gccttgtggt tctggaacca gtagaagacg 2880 ttcttaccct cgatcttacc gaattgacgc aggcgagcgg tgatcttctg aatctgatca 2940 gcagtagggg agcggatggc attgttgtag tacaactcct tcaggatctt gatctgctca 3000 gtggtaggtg tccatctggt ggaggtctgt ctgcaagtgt accctccgga accggacttg 3060 ttgttgttgt tgttcccgga ctcttgatcg gcctgatggt ggtgatgttg gtgctgagga 3120 ggctccatgg tatgattatt gctacggatc ttttaaggtt ccggagagag gattatagtt 3180 tctcaatcta acaaaaacac aattcaaaca catgtagtat agtacggttc tctctcagag 3240 aaaaagacac aacaaacata tcaacatttt cttttctttc tttcttgttt tttttaaaga 3300 aagtttaatt ttttttttct tttgactttt ttatggagaa aaataacgca gttttgggga 3360 aacgcggatt tgtaatatcg agaaggatat ttggtctggt tggtttaatt tgtgatgcaa 3420 atgtgatcga ctgatcgtta aaaaagtgaa aattttgaga tgggtcaatt ttgtttcaaa 3480 aattttaatg aaaaaagggt gaaaaattaa agaggtggac aattgtgaaa aaaaaaaaaa 3540 agagaaggta agagagtcaa aagagttgaa agagaaggtg aaaagttaaa agcaccgacc 3600 tgggcaagag tgaaaggtga tgaagatgat ccaaaaaacc ctccaacgtt gttgttgttc 3660 cttgctatca tcgaaggcat gtttctcgcc ggaatcatca taataacggc ttgcatactt 3720 cacatgcctc aaaaaccacc accaacaagt atatgtagaa ttgactcttt ctctctctat 3780 atatatcact ttctctctcc taagaggttc ttggatagag aaaaagctta agaagttgag 3840 cccttggatt ttctgtcaga tagcaatgtc aaagcgagta agtgtgtgtg ttactacaaa 3900 aaaactcagt gatacaaacg tgacctccaa gagaaaaaaa agattaaaga gaaaaaaact 3960 taatcaaaat ttcgaaacaa atatacgcta tgcatatatc atatcaaaat atatgaatta 4020 aaaagcaaag aaatattgta acccttatct gatcttgtct tttcgcaaaa caaaatcaaa 4080 acttttttaa agaaaatata acacccacga agatctcgga gaaaatccta caacgcatat 4140 cctatatatc aggatatttt tctttttttt tcttgcagac ctaataacgt tccttttctc 4200 acagccctag ctacaagtga ctcacctgaa aaatgttaga actcacaaaa aaaaaaaaaa 4260 aaattgtacc aaacagtttc attagttagt ttcttttttt tttctttaaa aaaaaacaat 4320 gaccttagac aaccttaaca aaaaaaacat gaatgttaca gagattcacg cagtgaccct 4380 ggttgcgaca gaagttttac aaacaagatt ttttttaact taccagtgag agtgggagta 4440 gaagatgaag gtgtagatct gttttgggtt gaaaagagat gaccgttttg aaggaagggt 4500 ttgggacagt tttgaaaaaa aaaatgttta cttatatggt aaaaaagaaa agaaaaagaa 4560 gctatggcat gaagaggtgt tacagagaga aagtgaggag aagaagaaga gatagggaca 4620 agggcattga aggaggagag ggaagtgggg aacaaatgga ggaaaatgtg gcagcgaaga 4680 gtaattgcag agcgtattga gagggtgcaa ttgcatttaa tggagggcaa aagaggtgta 4740 attgatagag aggggagaga gaaagagacg cagcatattt ttggtgtata aaactactaa 4800 taaatttttt gcgagttgac ggtgcaatgc aacgaatgac ggctaccaaa caaatgctac 4860 taaattgcta ctaagtgctg gtatcttgaa attaatttgt gcgtgtcact cccattcatt 4920 tcttcttctc ttttgcagtt tttccgatcg gataagtata gagtttgttt ttttttcccc 4980 cattaagaca agtgtaattg gttcaagaat tgaaagagtt cttagtattg aaattaaatg 5040 ttattaatgt gctttaatgt gatttttaat atgtttttaa tgtcactttt aatgatgttt 5100 tatgaatata tatatatata tatatatata tatatatata tatataatat tacatattaa 5160 aaatctttta gatatgattt ttaaagtaat tattataaaa ataagaatat tatcttacat 5220 gataaattat acactattta aaatatattt aacaagatat tttaattagt ttttaatttt 5280 tttactagtt taaaaatttg gtaaataagt ttttatttaa caactctaag gatttttgaa 5340 acactaaagt tatattttta aaaatgttag tttttttttg taacttttta tatttattat 5400 gttattgttg ttgaatattt attagaattt tttttaatct attttatata aggaaaaaat 5460 tcattatttt taacttttta tatataatag agttttctca tttttaccat tttattatat 5520 atgatattga tagtgatata taataaaata gaagttgata tattaaaaag tgttacctta 5580 agtaacgttt taaaaaatgt tagaagttat taaaaatttt aagttatatt ttttaaaaca 5640 ctagttttta taactttttg tatttattct tttttgttct ttaacattta taagttttcc 5700 tttttaatct tttttattta agaaaaaaaa attaactttt ttacatatga tagaattttc 5760 tcatttttac cattttatta tatatgatat tgatggtgat atataataag tttatgtaga 5820 tttttttata tttcataaat aaaaagctac aaatgtttaa aattaattta atactaacat 5880 tataaatatt atagttcttt ttatagaaaa aagtcaaatt aaaagttgta taaaaatatg 5940 tccatgtaat tttatattgt tcagttactt taataaataa ttttatcaac cattttatga 6000 tttaataaga tatattttca acattcaact actaattaac tttttgactt tagttagttt 6060 tgtcaaaact agtaaaaaaa ctagctagaa gtaggcaagc tagttggtaa accggaaact 6120 aaaaaattag tttgttgaag taggtgaaaa atataaaatg ataaaaaaaa taataaaatc 6180 ataatttatt ttaagaaaag ataacaaaga aaataaataa atatattaat aataaaattg 6240 aaagaaaata taaaaaatta gaattcagaa ggtaatatct taaaagaatg ttaggtgttt 6300 caaaaaacgt taaaatacta ctaaaaaact cccgggccat tctggcccac tttgtacaag 6360 aaagctgggt gcccggggtt aaccttcagc ttagaaatga tgtgttttct acacttttct 6420 cttctttttt ttcttttcat ctcacatgaa tcaaaaagag aaagcttaat tacattaaag 6480 gttgatgtga tatttacttt ctaaatattt gctagaagaa attataatga taacattaaa 6540 ttaagttata gtcatttcta tttcggttgt ctcccacttt ctgctatgct tttacaacaa 6600 cagttcaaga tttggaatcg ttttctttct ccaccaatcg tagtaatact tctttttgtg 6660 tttgttgaaa ttttgcaaga ttctctcttc ctctggttaa tacacataac ttacttggta 6720 agcttggctg cattttcttt tcaagctttt aatcaaagac tcactagaaa ttgcaaaggc 6780 aaacataaaa ttatgtgatc aaactcctac tgtgagtttt tggttacaaa caaaagttaa 6840 aaacaaaagc ttgatattta aaagataaac tcaacacata tgcatttgac tggcgcacag 6900 aagacaattc aagacttggg aaaacagtga gccatcaaaa tcgtcagata attcagggaa 6960 tatattgaat gtggagatgt ggtaatcaca ctattgcaca caaagagtgg caaacaagcg 7020 gcgcccttgg tttggtatag taagagacaa ttgttttgga ctgtttgtgt tgccatattc 7080 acaaaaatac tattgagcta tgtatgttaa acattatggt caatgagtag tagtgaacat 7140 tcaagttttt ttttttttcc acttcaagtt tttcatctta ttattatttt tcgttgtgat 7200 gcagtagtac ggcccatctt caccagtagt atatcaaagc cgaaacagat gacaaattgt 7260 ttaacatgca cttggattat tattgagagt tgaaatacaa aggcccgtta caagcccaaa 7320 cgaaggtaca taaaagaaaa gcagaaaatg aaaacgaaat cgatagggat aagtttttcc 7380 aactgacgga aacacaaact acaccaggtg aaatagatca cctcccaagt gcatgagaca 7440 cgaaccaacc acttatcatc agatcacaaa agagctcaaa gatctagcga tcgcctaatt 7500 cagtttgtgg ccaagctttg atggcaagtc gcaatatcta gcgacggcca cctcgtgctg 7560 ctccacataa gtctccttgt cggcctcttt tatgcgctcg agtctatggt ccacgtaata 7620 cacgccgggc attttcaagt ttttggcagg ctttttggag cggtaggtgg tcttgaagtt 7680 acagatgaga tggcctccgc cgacaagctt caaggccatg tcggatctac cttcaagacc 7740 accgtcggct gggtaaagca tttcagtatt ggcttcccag ccgagagtct tcttctgcat 7800 cacgggaccg tttgatggga agttcacgcc gcggatcttg acgttgtaga tcaagcagcc 7860 gtcttgaaga ctagtgtctt gggtggcggt caagactcct ccgtcttcgt aggtggtcac 7920 tctctcccag gtgaatccct ctgggaatga ctgtttgaag aagtctggga tgccctgggt 7980 gtggttgatg aaggttctac tcccgtacat gaaggaggtg gctagtatgt cgaaggcgaa 8040 tgggagtgga cctccttcga ccaccttgat tctcatagtc tgggttccct catagggctt 8100 gccctcaccc tcagaggtac acttgaagtg gtgattattc acggtgccct ccatgtataa 8160 cttcatgtgc atgttctctt taatgagctc ctcccccttg gacaccatgg ctgtagagtc 8220 aacaatcaca agataaatca gaatcaaaat ccatcacgta atttcaagaa gcaatttcgt 8280 gaaaaaaaat cctaatctat ggccacagaa tcaacaaata aggtagttgc taaaggacct 8340 gagcaatatc tcttgaaata ccatacagaa tcaacgaata aggcttttat caacggatct 8400 gaacaatttc ttgaaaaacg actgaagtat caacaaataa ggctgttgtt taacggatct 8460 gaagtagttc atgaaaaaaa ccttaatcta cgactacaga atcaacaatt aaggcttgtt 8520 gtaaacggat ctgaaaatct ttatgaaaaa aactctaatt agacctaatt tacggttata 8580 aaatcatcaa ttaaggacgt tcttgcatta ataaaagctg aatcttctga ttatgaaaat 8640 ttataatcga tgaaacgata gaaaacacaa tcaaatcaac atcatgctga tgtagaacaa 8700 acaataaaga agaagaaaaa aaaacggaag aaatctacga accttgaaag gagaggtagg 8760 gaactgcgca aagagaatga actaatttga ttgcaattga agtgcgatta gggtcgaggg 8820 gtgtgggggc attttatagc atatgcggtg gaaccgttct ccaatcatat tgtgacgcgt 8880 gcccatgtat ggtgtgactt ggtgtttatg acacagatga tatttcgggg agtccttcgt 8940 agttgacggt ttcgtaatct tgacggagtc agagatgtac cttcaccgct tcacgcttgg 9000 ctttatccgt taccacggcg ttattaaatt gggcccgcac ccgttgcaag ttaggaccac 9060 agtgtccatt acctgaacgg tcggctgcta ccagtatttt acattattaa aatctatgga 9120 aaagctgtca cgctagattt tttttttttt ttttatggat aagaaaagac taataggaaa 9180 gctttcttac tccactaaag tctttaataa ttattttttc aattttcaaa taaaatcata 9240 atcatcccac taaattcttt ctcatttgtt tttcactttg taagatattc ttccgtagat 9300 ttttataagt aattctttaa gatacatagc ataataaatt gtatactttt agttacagtc 9360 tatataaaat tagtcattta aaaaatatag ccttaatgat ttttttaaaa tgaagttatt 9420 tttttctaat ttttctttct tatttattat cgaatgattt tttctactaa tcgtttaggg 9480 aagagtttta tttttctaat gacgagatgt gttaaatgat gcaacttgaa ggcgacatat 9540 tttttaccaa taaaataaaa ctaaaagata gcttgttgac aaaaacaata attgatgtta 9600 aagaaaacgt aagggcaatg ggtatcatct aattgttgga caagttgtct tctaaggcta 9660 ggtaagcttt tgttttttat tagctgaaat atttatttaa ttgttctata aataaatttt 9720 ttaaaatgat ttttaatgtt tttaaatatt atttaaaata atttttttaa aattttaaca 9780 ttttagtttt taattatttt ttatcttact taaattaatt ataatttatt attttctacc 9840 attttatact ttccaattaa ttttatcgaa gcattaatac catctggtat tttgtcactg 9900 cttcgtatga ttgtttatta aattttagct tctttcacat tggagatgct gggcggtgtg 9960 agttacagca tggtgtgaag ttaaggtggt ttgattatgg aacccgaagg tgttggtgca 10020 aggtggataa aattgcttgc caggagatac ataaaatgat ttacaaaatg aaataaagaa 10080 taaataaata gtcaagttca aatttaaatc aaggattaga attatttttc cgtacatgat 10140 gtaaccgcaa tcaccccctg ctgaatccag atggccattc tggccgaatt taagtgacta 10200 gggtcaccct gctgaatcca gatggccatt ctggccgaat ttaagtgact agggtcacgt 10260 gaccctagtc acttaccgga ttctggccgt accgagctcg aattcggccc aacttttcta 10320 tacaaagttg aggtacgaga tccgcccatc cggccggcca gatcctgcag gagatccaag 10380 cttggcgcgc cgcggacctg aattccagca cactggcggc cgttactagt aacggccgcc 10440 agtgtgctgg aattcgccct tcccaagctt tgctctagat caaactcaca tccaaacata 10500 acatggatat cttccttacc aatcatacta attattttgg gttaaatatt aatcattatt 10560 tttaagatat taattaagaa attaaaagat tttttaaaaa aatgtataaa attatattat 10620 tcatgatttt tcatacattt gattttgata ataaatatat tttttttaat ttcttaaaaa 10680 atgttgcaag acacttatta gacatagtct tgttctgttt acaaaagcat tcatcattta 10740 atacattaaa aaatatttaa tactaacagt agaatcttct tgtgagtggt gtgggagtag 10800 gcaacctggc attgaaacga gagaaagaga gtcagaacca gaagacaaat aaaaagtatg 10860 caacaaacaa atcaaaatca aagggcaaag gctggggttg gctcaattgg ttgctacatt 10920 caattttcaa ctcagtcaac ggttgagatt cactctgact tccccaatct aagccgcgga 10980 tgcaaacggt tgaatctaac ccacaatcca atctcgttac ttaggggctt ttccgtcatt 11040 aactcacccc tgccacccgg tttccctata aattggaact caatgctccc ctctaaactc 11100 gtatcgcttc agagttgaga ccaagacaca ctcgttcata tatctctctg ctcttctctt 11160 ctcttctacc tctcaaggta cttttcttct ccctctacca aatcctagat tccgtggttc 11220 aatttcggat cttgcacttc tggtttgctt tgccttgctt tttcctcaac tgggtccatc 11280 taggatccat gtgaaactct actctttctt taatatctgc ggaatacgcg tttgactttc 11340 agatctagtc gaaatcattt cataattgcc tttctttctt ttagcttatg agaaataaaa 11400 tcactttttt tttatttcaa aataaacctt gggccttgtg ctgactgaga tggggtttgg 11460 tgattacaga attttagcga attttgtaat tgtacttgtt tgtctgtagt tttgttttgt 11520 tttcttgttt ctcatacatt ccttaggctt caattttatt cgagtatagg tcacaatagg 11580 aattcaaact ttgagcaggg gaattaatcc cttccttcaa atccagtttg tttgtatata 11640 tgtttaaaaa atgaaacttt tgctttaaat tctattataa ctttttttat ggctgaaatt 11700 tttgcatgtg tctttgctct ctgttgtaaa tttactgttt aggtactaac tctaggcttg 11760 ttgtgcagtt tttgaagtat aacaacagaa gttcctattc cgaagttcct attctctaga 11820 aagtatagga acttccacca cacaacacaa tggcggccac cgcttccaga accacccgat 11880 tctcttcttc ctcttcacac cccaccttcc ccaaacgcat tactagatcc accctccctc 11940 tctctcatca aaccctcacc aaacccaacc acgctctcaa aatcaaatgt tccatctcca 12000 aaccccccac ggcggcgccc ttcaccaagg aagcgccgac cacggagccc ttcgtgtcac 12060 ggttcgcctc cggcgaacct cgcaagggcg cggacatcct tgtggaggcg ctggagaggc 12120 agggcgtgac gacggtgttc gcgtaccccg gcggtgcgtc gatggagatc caccaggcgc 12180 tcacgcgctc cgccgccatc cgcaacgtgc tcccgcgcca cgagcagggc ggcgtcttcg 12240 ccgccgaagg ctacgcgcgt tcctccggcc tccccggcgt ctgcattgcc acctccggcc 12300 ccggcgccac caacctcgtg agcggcctcg ccgacgcttt aatggacagc gtcccagtcg 12360 tcgccatcac cggccaggtc gcccgccgga tgatcggcac cgacgccttc caagaaaccc 12420 cgatcgtgga ggtgagcaga tccatcacga agcacaacta cctcatcctc gacgtcgacg 12480 acatcccccg cgtcgtcgcc gaggctttct tcgtcgccac ctccggccgc cccggtccgg 12540 tcctcatcga cattcccaaa gacgttcagc agcaactcgc cgtgcctaat tgggacgagc 12600 ccgttaacct ccccggttac ctcgccaggc tgcccaggcc ccccgccgag gcccaattgg 12660 aacacattgt cagactcatc atggaggccc aaaagcccgt tctctacgtc ggcggtggca 12720 gtttgaattc cagtgctgaa ttgaggcgct ttgttgaact cactggtatt cccgttgcta 12780 gcactttaat gggtcttgga acttttccta ttggtgatga atattccctt cagatgctgg 12840 gtatgcatgg tactgtttat gctaactatg ctgttgacaa tagtgatttg ttgcttgcct 12900 ttggggtaag gtttgatgac cgtgttactg ggaagcttga ggcttttgct agtagggcta 12960 agattgttca cattgatatt gattctgccg agattgggaa gaacaagcag gcgcacgtgt 13020 cggtttgcgc ggatttgaag ttggccttga agggaattaa tatgattttg gaggagaaag 13080 gagtggaggg taagtttgat cttggaggtt ggagagaaga gattaatgtg cagaaacaca 13140 agtttccatt gggttacaag acattccagg acgcgatttc tccgcagcat gctatcgagg 13200 ttcttgatga gttgactaat ggagatgcta ttgttagtac tggggttggg cagcatcaaa 13260 tgtgggctgc gcagttttac aagtacaaga gaccgaggca gtggttgacc tcagggggtc 13320 ttggagccat gggttttgga ttgcctgcgg ctattggtgc tgctgttgct aaccctgggg 13380 ctgttgtggt tgacattgat ggggatggta gtttcatcat gaatgttcag gagttggcca 13440 ctataagagt ggagaatctc ccagttaaga tattgttgtt gaacaatcag catttgggta 13500 tggtggttca gttggaggat aggttctaca agtccaatag agctcacacc tatcttggag 13560 atccgtctag cgagagcgag atattcccaa acatgctcaa gtttgctgat gcttgtggga 13620 taccggcagc gcgagtgacg aagaaggaag agcttagagc ggcaattcag agaatgttgg 13680 acacccctgg cccctacctt cttgatgtca ttgtgcccca tcaggagcat gtgttgccga 13740 tgattcccag taatggatcc ttcaaggatg tgataactga gggtgatggt agaacgaggt 13800 actgattgcc tagaccaaat gttccttgat gcttgttttg tacaatatat ataagataat 13860 gctgtcctag ttgcaggatt tggcctgtgg tgagcatcat agtctgtagt agttttggta 13920 gcaagacatt ttattttcct tttatttaac ttactacatg cagtagcatc tatctatctc 13980 tgtagtctga tatctcctgt tgtctgtatt gtgccgttgg attttttgct gtagtgagac 14040 tgaaaatgat gtgctagtaa taatatttct gttagaaatc taagtagaga atctgttgaa 14100 gaagtcaaaa gctaatggaa tcaggttaca tattcaatgt ttttcttttt ttagcggttg 14160 gtagacgtgt agattcaact tctcttggag ctcacctagg caatcagtaa aatgcatatt 14220 ccttttttaa cttgccattt atttactttt agtggaaatt gtgaccaatt tgttcatgta 14280 gaacggattt ggaccattgc gtccacaaaa cgtctctttt gctcgatctt cacaaagcga 14340 taccgaaatc cagagatagt tttcaaaagt cagaaatggc aaagttataa atagtaaaac 14400 agaatagatg ctgtaatcga cttcaataac aagtggcatc acgtttctag ttctagaccc 14460 atcagctgag gtacaaatta attaataagt gactagggtc acgtgaccct agtcacttat 14520 gattccgatg acttcgtagg ttcctagctc aagccgctcg tgtccaagcg tcacttacga 14580 ttagctaatg attacggcat ctaggaccga ctagttctta tgtgcttcta gtctccaaat 14640 gtggttgata gttattttgc tctaagatca acagtaatga agtataaatc atcgttgtgg 14700 tgtgctactc ggttaattga gcattaacac acacaaacat gacgaggatg gtataatctc 14760 caaaaatgtg tactttgtta ggtgggaccc tatagccttg attaatgtgc tatgttaggc 14820 atgcctggaa acgtgtgacg catatgtttt gtgaacctgt tgatattata tgtgctttta 14880 tattaccata ttttattaaa atactaatat ttattactag taagatataa cattctatct 14940 agcttaaaaa ctaaccataa atattccata ataactagat ttaccaaact aatatactaa 15000 atatacataa taaatacaaa attaacaaga caataatcaa tatttatgag cttaatatat 15060 ttagacatta tggttggtcg acgataatca tgctaacttt tcgtaattgc ttgattgaaa 15120 tatgcttaga ataatgcctc tttgttctac atggcaaata gggaccatta tggtgtaaca 15180 ccctgggaac cacaaacacc ccgaaatgct actaaactac acaactaacc ttcatatata 15240 aaatttcgac agcatctcct ttgaaaattt gcatagacgt ggaagcaaca gagtataaac 15300 agatctagct agctaagttt aaacggccgg ccatttacaa ttgaatatat cctgcca 15357 <210> 117 <211> 9769 <212> DNA <213> Artificial Sequence <220> <223> plasmid QC318 <400> 117 tttgtacaaa cttgtgattc ttccttacca atcatactaa ttattttggg ttaaatatta 60 atcattattt ttaagatatt aattaagaaa ttaaaagatt ttttaaaaaa atgtataaaa 120 ttatattatt catgattttt catacatttg attttgataa taaatatatt ttttttaatt 180 tcttaaaaaa tgttgcaaga cacttattag acatagtctt gttctgttta caaaagcatt 240 catcatttaa tacattaaaa aatatttaat actaacagta gaatcttctt gtgagtggtg 300 tgggagtagg caacctggca ttgaaacgag agaaagagag tcagaaccag aagacaaata 360 aaaagtatgc aacaaacaaa tcaaaatcaa agggcaaagg ctggggttgg ctcaattggt 420 tgctacattc aattttcaac tcagtcaacg gttgagattc actctgactt ccccaatcta 480 agccgcggat gcaaacggtt gaatctaacc cacaatccaa tctcgttact taggggcttt 540 tccgtcatta actcacccct gccacccggt ttccctataa attggaactc aatgctcccc 600 tctaaactcg tatcgcttca gagttgagac caagacacac tcgttcatat atctctctgc 660 tcttctcttc tcttctacct ctcaaggtac ttttcttctc cctctaccaa atcctagatt 720 ccgtggttca atttcggatc ttgcacttct ggtttgcttt gccttgcttt ttcctcaact 780 gggtccatct aggatccatg tgaaactcta ctctttcttt aatatctgcg gaatacgcgt 840 ttgactttca gatctagtcg aaatcatttc ataattgcct ttctttcttt tagcttatga 900 gaaataaaat cacttttttt ttatttcaaa ataaaccttg ggccttgtgc tgactgagat 960 ggggtttggt gattacagaa ttttagcgaa ttttgtaatt gtacttgttt gtctgtagtt 1020 ttgttttgtt ttcttgtttc tcatacattc cttaggcttc aattttattc gagtataggt 1080 cacaatagga attcaaactt tgagcagggg aattaatccc ttccttcaaa tccagtttgt 1140 ttgtatatat gtttaaaaaa tgaaactttt gctttaaatt ctattataac tttttttatg 1200 gctgaaattt ttgcatgtgt ctttgctctc tgttgtaaat ttactgttta ggtactaact 1260 ctaggcttgt tgtgcagttt ttgaagtata accatgccac acaacacaat ggcggccacc 1320 gcttccagaa ccacccgatt ctcttcttcc tcttcacacc ccaccttccc caaacgcatt 1380 actagatcca ccctccctct ctctcatcaa accctcacca aacccaacca cgctctcaaa 1440 atcaaatgtt ccatctccaa accccccacg gcggcgccct tcaccaagga agcgccgacc 1500 acggagccct tcgtgtcacg gttcgcctcc ggcgaacctc gcaagggcgc ggacatcctt 1560 gtggaggcgc tggagaggca gggcgtgacg acggtgttcg cgtaccccgg cggtgcgtcg 1620 atggagatcc accaggcgct cacgcgctcc gccgccatcc gcaacgtgct cccgcgccac 1680 gagcagggcg gcgtcttcgc cgccgaaggc tacgcgcgtt cctccggcct ccccggcgtc 1740 tgcattgcca cctccggccc cggcgccacc aacctcgtga gcggcctcgc cgacgcttta 1800 atggacagcg tcccagtcgt cgccatcacc ggccaggtcg cccgccggat gatcggcacc 1860 gacgccttcc aagaaacccc gatcgtggag gtgagcagat ccatcacgaa gcacaactac 1920 ctcatcctcg acgtcgacga catcccccgc gtcgtcgccg aggctttctt cgtcgccacc 1980 tccggccgcc ccggtccggt cctcatcgac attcccaaag acgttcagca gcaactcgcc 2040 gtgcctaatt gggacgagcc cgttaacctc cccggttacc tcgccaggct gcccaggccc 2100 cccgccgagg cccaattgga acacattgtc agactcatca tggaggccca aaagcccgtt 2160 ctctacgtcg gcggtggcag tttgaattcc agtgctgaat tgaggcgctt tgttgaactc 2220 actggtattc ccgttgctag cactttaatg ggtcttggaa cttttcctat tggtgatgaa 2280 tattcccttc agatgctggg tatgcatggt actgtttatg ctaactatgc tgttgacaat 2340 agtgatttgt tgcttgcctt tggggtaagg tttgatgacc gtgttactgg gaagcttgag 2400 gcttttgcta gtagggctaa gattgttcac attgatattg attctgccga gattgggaag 2460 aacaagcagg cgcacgtgtc ggtttgcgcg gatttgaagt tggccttgaa gggaattaat 2520 atgattttgg aggagaaagg agtggagggt aagtttgatc ttggaggttg gagagaagag 2580 attaatgtgc agaaacacaa gtttccattg ggttacaaga cattccagga cgcgatttct 2640 ccgcagcatg ctatcgaggt tcttgatgag ttgactaatg gagatgctat tgttagtact 2700 ggggttgggc agcatcaaat gtgggctgcg cagttttaca agtacaagag accgaggcag 2760 tggttgacct cagggggtct tggagccatg ggttttggat tgcctgcggc tattggtgct 2820 gctgttgcta accctggggc tgttgtggtt gacattgatg gggatggtag tttcatcatg 2880 aatgttcagg agttggccac tataagagtg gagaatctcc cagttaagat attgttgttg 2940 aacaatcagc atttgggtat ggtggttcag ttggaggata ggttctacaa gtccaataga 3000 gctcacacct atcttggaga tccgtctagc gagagcgaga tattcccaaa catgctcaag 3060 tttgctgatg cttgtgggat accggcagcg cgagtgacga agaaggaaga gcttagagcg 3120 gcaattcaga gaatgttgga cacccctggc ccctaccttc ttgatgtcat tgtgccccat 3180 caggagcatg tgttgccgat gattcccagt aatggatcct tcaaggatgt gataactgag 3240 ggtgatggta gaacgaggta ctgattgcct agaccaaatg ttccttgatg cttgttttgt 3300 acaatatata taagataatg ctgtcctagt tgcaggattt ggcctgtggt gagcatcata 3360 gtctgtagta gttttggtag caagacattt tattttcctt ttatttaact tactacatgc 3420 agtagcatct atctatctct gtagtctgat atctcctgtt gtctgtattg tgccgttgga 3480 ttttttgctg tagtgagact gaaaatgatg tgctagtaat aatatttctg ttagaaatct 3540 aagtagagaa tctgttgaag aagtcaaaag ctaatggaat caggttacat attcaatgtt 3600 tttctttttt tagcggttgg tagacgtgta gattcaactt ctcttggagc tcacctaggc 3660 aatcagtaaa atgcatattc cttttttaac ttgccattta tttactttta gtggaaattg 3720 tgaccaattt gttcatgtag aacggatttg gaccattgcg tccacaaaac gtctcttttg 3780 ctcgatcttc acaaagcgat accgaaatcc agagatagtt ttcaaaagtc agaaatggca 3840 aagttataaa tagtaaaaca gaatagatgc tgtaatcgac ttcaataaca agtggcatca 3900 cgtttctagt tctagacccg ggtaccggcg cgcccgatca tccggatata gttcctcctt 3960 tcagcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct agttattgct 4020 cagcggtggc agcagccaac tcagcttcct ttcgggcttt gttagcagcc ggatcgatcc 4080 aagctgtacc tcactattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat 4140 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg 4200 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag 4260 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga 4320 gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc 4380 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt 4440 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt 4500 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt 4560 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt 4620 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc 4680 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa 4740 gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ctgcagaaca gcgggcagtt 4800 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca 4860 ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa 4920 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga 4980 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct 5040 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg 5100 tgagttcagg cttttccatg ggtatatctc cttcttaaag ttaaacaaaa ttatttctag 5160 agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctg 5220 atcaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5280 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5340 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5400 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5460 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5520 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5580 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5640 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5700 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5760 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5820 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5880 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 5940 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6000 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 6060 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 6120 gtcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc 6180 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 6240 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 6300 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatggac 6360 atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga 6420 tttaggtgac actatagaac ggcgcgccgg taccgggccc cccctcgagt gcggccgcaa 6480 gcttgtcgac ggagatcacc actttgtaca agaaagctgg gtctagatat ctcgacccgg 6540 gcaatcaaat tatatatgta aagcaattac agtttatcaa actttattta tggaaataat 6600 ttattatcac atttattttg gtttataaat tttaaattaa aatatcacct aaataaaaat 6660 aatttttaac atgacttatt gtcctaaata aattatttcc gtaaattaaa taaaatgaag 6720 tttttttctt tcaaagaatc taaatggtca taatgagaat tctctaaaaa aatacataat 6780 gagaataatt atggaattta tttattaata aaaattaata gcattttgat agacaattaa 6840 taaaatttta aaaataacca tatagaaata ataatttttt tactatcggt tccaattaaa 6900 ataatgataa aaaataaaat agattattaa ttgatattga tatgaaattt aaataaagaa 6960 tataatcata tattttattg atatatgata tgatatagat taattgatat tgattttgat 7020 atggaattta aaaataatat aataattgtt tttatttatt aatacgtgta atcaaataat 7080 tctcactttt tgaatcaatc agtgtactta aagataatat cagttgaata ttttttatcc 7140 ttttacgtgt gctgtgagac attatcatca attgtgttgt atatgatata tagatataga 7200 tatataaata tatagattga gtgatataat atatttaaaa tataaattat atatatgttt 7260 taatatattt ttgcatatat atatatattt gtaaaaacta gaagtatttt ttcatgagat 7320 aattattatc gagttgaata agtctattat ttgtgagagc caaccatatt tatatatgtg 7380 attaaatttt atctttgtga aattaaaaat aataaaaaat accttaaaat cataataata 7440 gaaaaactta tatttataat ttaccattat acttaaaaaa aattaaataa atattataaa 7500 tataaatact atcgagtaat ggccgcgcta gggtttttga gaaaaaatct tcccacgcac 7560 tcaactgcac tgtacggcgt cgttttcaca gccgcataat agaagccgcg ttccccaacc 7620 cttcctcaca acattctcgg accctccagc accgtcaccc aaacaaatat ccacgcggta 7680 gtaggcgcgt gaaacaaact ctaatccgaa ctacgagacg tgagaagcac gcgctttagc 7740 gagcgtttca attgtcgcta cgaaagcaga gaaggataca aacggaacta gggtaaatta 7800 gtaagggtaa tttcgtaaac agaagaaaag agttgtagct ataaataaac cctctaaccc 7860 tcgtcgcatt acttctcttc acacctttgt tcactcttct tctcttgcgg ctagggtttt 7920 agcgcagctt cttctaggtt cgttatctac caccgttcta tggattttat tccttctatt 7980 cgtgtttatt ctattggttt atgttgcttg caatatgttt tttctgaatc tgtcgtcgtt 8040 gtcttcaatt ttatccatgt ttcagagatc aattttgttt gtgtagtatg tgcttattct 8100 tcttcttttc gttcgagttg ttaataacgg tgctatggtg ttttcaaaag tgtttttttt 8160 attacttttg atttaaagtt tttttggtaa ggcttttatt tgcttgttat attcaaatct 8220 ttggatccag atcttatata agtttttggt tcaagaaagt ttttggttac tgatgaatag 8280 atctattaac tgttacttta atcgattcaa gctaaagttt tttggttact gatgaataga 8340 tctattatct gttactttta atcggttcaa gctcaagttt tttggttact gatgaataga 8400 tctatatacg tcacagtgtg ctaaacatgc ccttgtttta tctcgatctt atgtatggga 8460 gtgccataaa ttttgttatg tctatttttt tatctgttgg aatcatactg agtttgatgc 8520 gttacgattg agcataccta tttttgggct tgttgtatgg tgggtattta gatcttaatc 8580 tttttatgct tatgaaaggt tttgtaatga caaaggtctt aatgttgtta aacttttatt 8640 tttactttat atggtgtgtt gatgtgttat ggttttgaca actttttttt tttctggatt 8700 tttgcagatt taaggaagcc atggcccaca gcaagcacgg cctgaaggag gagatgacca 8760 tgaagtacca catggagggc tgcgtgaacg gccacaagtt cgtgatcacc ggcgagggca 8820 tcggctaccc cttcaagggc aagcagacca tcaacctgtg cgtgatcgag ggcggccccc 8880 tgcccttcag cgaggacatc ctgagcgccg gcttcaagta cggcgaccgg atcttcaccg 8940 agtaccccca ggacatcgtg gactacttca agaacagctg ccccgccggc tacacctggg 9000 gccggagctt cctgttcgag gacggcgccg tgtgcatctg taacgtggac atcaccgtga 9060 gcgtgaagga gaactgcatc taccacaaga gcatcttcaa cggcgtgaac ttccccgccg 9120 acggccccgt gatgaagaag atgaccacca actgggaggc cagctgcgag aagatcatgc 9180 ccgtgcctaa gcagggcatc ctgaagggcg acgtgagcat gtacctgctg ctgaaggacg 9240 gcggccggta ccggtgccag ttcgacaccg tgtacaaggc caagagcgtg cccagcaaga 9300 tgcccgagtg gcacttcatc cagcacaagc tgctgcggga ggaccggagc gacgccaaga 9360 accagaagtg gcagctgacc gagcacgcca tcgccttccc cagcgccctg gcctgagagc 9420 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt 9480 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt 9540 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta 9600 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc 9660 gcggtgtcat ctatgttact agatcgggaa ttctagtggc cggcccagct gatatccatc 9720 acactggcgg ccgcactcga ctgaattggt tccggcgcca gcctgcttt 9769 <210> 118 <211> 6538 <212> DNA <213> Artificial Sequence <220> <223> plasmid pVER9662 <400> 118 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 60 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 120 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 180 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 240 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 300 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg 360 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 420 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 480 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 540 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt aaggtgcacg gccctacagg 600 tcactaatac catctaagta gttggttcat agtgactgca tatgttgtgt tttacagtat 660 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 720 gtttctcgtt caactttctt gtacaaagtg gccgttaacg gatctggccg cggccgcatc 780 aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg atgatctagg 840 accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt gtatcattct 900 tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac gagtgttaaa 960 tatggaccag gccccaaata agatccattg atatatgaat taaataacaa gaataaatcg 1020 agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat acaaaagtca 1080 ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa aattaaaaga 1140 aatggataat ttcacaatat gttatacgat aaagaagtta cttttccaag aaattcactg 1200 attttataag cccacttgca ttagataaat ggcaaaaaaa aacaaaaagg aaaagaaata 1260 aagcacgaag aattctagaa aatacgaaat acgcttcaat gcagtgggac ccacggttca 1320 attattgcca attttcagct ccaccgtata tttaaaaaat aaaacgataa tgctaaaaaa 1380 atataaatcg taacgatcgt taaatctcaa cggctggatc ttatgacgac cgttagaaat 1440 tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg gcacacacga 1500 gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata aggcaattag 1560 ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat tattagctat 1620 tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt cttcttcttc 1680 tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat ttctcaaaat 1740 cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg tgttccttat 1800 tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt 1860 tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta gatatcatct 1920 taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt tgagttttgt 1980 cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt tctagtttgt 2040 gcgatcgaat ttgtcgatta atctgagttt ttctgattaa cagccatgga gcctcctcag 2100 caccaacatc accaccatca ggccgatcaa gagtccggga acaacaacaa caacaagtcc 2160 ggttccggag ggtacacttg cagacagacc tccaccagat ggacacctac cactgagcag 2220 atcaagatcc tgaaggagtt gtactacaac aatgccatcc gctcccctac tgctgatcag 2280 attcagaaga tcaccgctcg cctgcgtcaa ttcggtaaga tcgagggtaa gaacgtcttc 2340 tactggttcc agaaccacaa ggctcgtgag aggcagaaga agcgtttcaa cgggactaac 2400 atgaccactc cttcctcatc acccaattct gttatgatgg ccgccaacga ccactaccat 2460 cctttgcttc atcaccacca cggagttcct atgcaaagac ccgcaaactc cgtcaacgtg 2520 aagttgaacc aggaccatca cctctaccac cacaacaagc cctacccctc tttcaacaac 2580 gggaatctca accatgcctc ctctggaacc gaatgtggag ttgtcaacgc ctccaacggc 2640 tacatgtctt cccacgtgta cggctctatg gagcaagatt gctccatgaa ctacaacaac 2700 gttggaggcg gatgggcaaa catggatcac cactactcct ccgctcccta caacttcttc 2760 gatagggcta aacctctctt cggcttggag ggacaccaag aagaagagga atgcggaggt 2820 gatgcttact tggaacacag aaggactctc cctctctttc caatgcacgg tgaggaccac 2880 atcaacggag gatctggcgc tatctggaag tatggacagt ccgaggtcag accatgcgct 2940 tccctggaat tgagactgaa ctagattggg acctaggaaa cttatctctg ttatgaatca 3000 gaagaagttc atgtctcgtt tcatttaaaa ctttggtggt ttgtgttttg gggccttgta 3060 aagcccctga tgaataattg ttcaactatg tttccgttcc tgtgttatac ctttctttct 3120 aatgagtaat gacatcaaac ttcttctgta ttgaaattat gtccttgtga gtctctttat 3180 catcgtttcg tctttacatt atatgtgcta cttttgtcta atgagcctga aaagtggctc 3240 caatggtacg cactggaaga tttgttggct tctggtagat atagcgacag tgttgagctt 3300 gtaatatcat gtctcttatt gctaaattag ttcctttctt aacagaaacc ttcaaagttt 3360 ttgtttttgt tttcatttac ctaatgtaca catacgctgg ccatgactaa caacatgtcc 3420 aggcttagag catatttttt tctagcttaa attgttaact tgtcattcag taaaatccga 3480 gaattgtgaa gctctaattg aagctaattc gttttataaa gtcagttaaa aagtatacta 3540 aattatccaa cttttcttca aaatctcaaa attctatgac aaaacgatag tctttgttta 3600 tgtcagtacc acaaagaggt ggaaaaaaac accaaaaaaa caataagcaa actatacact 3660 gagaagaaaa ataaaagaga gctcaataga tgttttatac taacggtaga ttagatcaaa 3720 gatccaagct ttactctaca tagagcagaa cccagaatcc cttcatatct cttttattct 3780 agcaccgata atctactgaa aagaagacac ttagagctct gtctctttgt caaagaagtc 3840 ccagccgtca tccagaagct ccttacgttc attaacagag gtaccggcgg ccgcgggcaa 3900 ctttattata caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac 3960 aggtcactat cagtcaaaat aaaatcatta tttggagctc ccaattcgta atcatggtca 4020 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 4080 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 4140 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 4200 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 4260 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 4320 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4380 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4440 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4500 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4560 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4620 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4680 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4740 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4800 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 4860 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4920 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4980 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 5040 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 5100 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 5160 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 5220 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 5280 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 5340 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 5400 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 5460 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 5520 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 5580 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 5640 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5700 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5760 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5820 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5880 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5940 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 6000 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 6060 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 6120 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 6180 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 6240 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 6300 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 6360 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 6420 cataaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 6480 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagc 6538 <210> 119 <211> 5950 <212> DNA <213> Artificial Sequence <220> <223> plasmid UBIGMWUS <400> 119 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 60 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 120 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 180 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 240 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 300 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 360 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 420 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 480 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 540 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 600 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 660 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 720 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 780 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 840 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 900 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 960 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 1020 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 1080 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 1140 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 1200 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta 1260 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg 1320 ccaagtttgt acaaaaaagc aggctccggc cagaatccgg taagtgacta gggtcacatc 1380 gaattcccat aggattgggg ccagaatggc cgcggccgca tcaggatatt cttgtttaag 1440 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc 1500 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat 1560 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa 1620 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc 1680 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa 1740 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat 1800 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg 1860 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag 1920 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag 1980 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc 2040 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc 2100 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca 2160 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt 2220 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct 2280 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa 2340 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca 2400 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat 2460 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta 2520 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt 2580 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga 2640 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat 2700 taatctgagt ttttctgatt aacagccatg gaacctcaac aacaacaaca acaagcacaa 2760 gggagccaac aacaacaaca aaacgaggat ggtggcagtg gaaaaggggg gtttctgagc 2820 aggcaaagta gtacacggtg gactccaaca aacgaccaga taagaatatt gaaggaactt 2880 tactacaaca atggaattag atccccgagt gcagagcaga ttcagaggat ctctgctagg 2940 ctgaggcagt acggtaagat tgaaggcaag aatgtctttt attggttcca gaaccacaaa 3000 gctcgagaaa ggcagaagaa aaggttcact tctgatcata atcataataa tgtccccatg 3060 caaagacccc caactaatcc ttctgctgct tggaaacctg atctagctga tcccattcac 3120 accaccaagt attgtaacat ctcttctact gcagggatct cttcggcatc atcttctgtt 3180 gagatggtta ctgtgggaca gatggggaat tatgggtatg gttctgtgcc tatggagaaa 3240 agttttaggg actgctcgat atcagctggg ggtagcagtg gccatgttgg attaataaac 3300 cacaacttgg ggtgggttgg tgtggaccca tataattcct caacctatgc caacttcttt 3360 gacaaaataa ggccaagtga tcaagaaacc cttgaagaag aagcagagaa cattggtgct 3420 actaagattg aaaccctccc tttattccct atgcacggtg aggacatcca cggctattgc 3480 aacctcaagt ctaattcgta taactatgat ggaaacggct ggtatcatac tgaagaaggg 3540 ttcaagaatg cttctcgtgc ttccttggag ctcagtctca actcctacac tcgcaggtct 3600 ccagattatg cttaaagatc cgtttttgtg atctgatgat aagtggttgg ttcgtgtctc 3660 atgcacttgg gaggtgatct atttcacctg gtgtagtttg tgtttccgtc agttggaaaa 3720 acttatccct atcgatttcg ttttcatttt ctgcttttct tttatgtacc ttcgtttggg 3780 cttgtaacgg gcctttgtat ttcaactctc aataataatc caagtgcatg ttaaacaatt 3840 tgtcatctgt ttcggctttg atatactact ggtgaagatg ggccgtacta ctgcatcaca 3900 acgaaaaata ataataagat gaaaaacttg aagtggaaaa aaaaaaaaac ttgaatgttc 3960 actactactc attgaccata atgtttaaca tacatagctc aatagtattt ttgtgaatat 4020 ggcaacacaa acagtccaaa acaattgtct cttactatac caaaccaagg gcgccgcttg 4080 tttgccactc tttgtgtgca atagtgtgat taccacatct ccacattcaa tatattccct 4140 gaattatctg acgattttga tggctcactg ttttcccaag tcttgaattg tcttctgtgc 4200 gccagtcaaa tgcatatgtg ttgagtttat cttttaaata tcaagctttt gtttttaact 4260 tttgtttgta accaaaaact cacagtagga gtttgatcac ataattttat gtttgccttt 4320 gcaatttcta gtgagtcttt gattaaaagc ttgaaaagaa aatgcagcca agcttaccaa 4380 gtaagttatg tgtattaacc agaggaagag agaatcttgc aaaatttcaa caaacacaaa 4440 aagaagtatt actacgattg gtggagaaag aaaacgattc caaatcttga actgttgttg 4500 taaaagcata gcagaaagtg ggagacaacc gaaatagaaa tgactataac ttaatttaat 4560 gttatcatta taatttcttc tagcaaatat ttagaaagta aatatcacat caacctttaa 4620 tgtaattaag ctttctcttt ttgattcatg tgagatgaaa agaaaaaaaa gaagagaaaa 4680 gtgtagaaaa cacatcattt ctaagctgaa ggcggccgcg gtcaccccat acgattggaa 4740 gcttcccggg cacccagctt tcttgtacaa agtgggcatt ataagaaagc attgcttatc 4800 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc 4860 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc 4920 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 4980 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 5040 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 5100 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc 5160 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 5220 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 5280 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt 5340 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 5400 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct 5460 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga 5520 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc 5580 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 5640 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 5700 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 5760 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga 5820 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg 5880 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg 5940 agcgtcagac 5950 <210> 120 <211> 5971 <212> DNA <213> Artificial Sequence <220> <223> plasmid UBIMTWUS <400> 120 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 60 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 120 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 180 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 240 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 300 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 360 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 420 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 480 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 540 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 600 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 660 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 720 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 780 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 840 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 900 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 960 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 1020 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 1080 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 1140 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 1200 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta 1260 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg 1320 ccaagtttgt acaaaaaagc aggctccggc cagaatccgg taagtgacta gggtcacatc 1380 gaattcccat aggattgggg ccagaatggc cgcggccgca tcaggatatt cttgtttaag 1440 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc 1500 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat 1560 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa 1620 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc 1680 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa 1740 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat 1800 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg 1860 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag 1920 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag 1980 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc 2040 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc 2100 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca 2160 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt 2220 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct 2280 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa 2340 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca 2400 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat 2460 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta 2520 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt 2580 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga 2640 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat 2700 taatctgagt ttttctgatt aacagccatg gaacaacctc agcagcagca gcaaccacag 2760 acccaacagc actctccaaa caacggaatc atggggtcca gacagagttc caccagatgg 2820 accccaacaa ccgaccagat ccgcattctc aaagacctct actacaacaa cggaataagg 2880 tctccaagtg ccgagcaaat ccagaggatc tccgcaaggc tcagacagta cgggaagatc 2940 gagggcaaga acgtcttcta ctggttccaa aaccacaaag ccagagagcg ccagaaaaag 3000 cgcttcacct ccgacgtcaa cgtcgtccct atcatccagc gcgcccccaa caacaatact 3060 atcataagtg ccgccaactg gaagcctgac caccacgaac aacagcagaa cattaatgtc 3120 cacacaaacc actccacata caacatctcc tccgctggcc tcagttctgc ttcctgctcc 3180 tcagcagaaa tggtcactgt ggggcaaata ggcaactacg gctacgggtc tgtcccaatg 3240 gagaagtctt tccgcgaatg caccatctca gctggatgct catcatccca ggttggctca 3300 accataaacc cccacatcgg ctggatcgga caccacgtcg acccatattc aagtgcctac 3360 gctaacctct tcgagaaaat acgccccaac gaggagatca tggaagagta cgaccaggga 3420 caggaaaacg gttccccaga gatcgagaca ctcccactct tccctatgca cggagaagac 3480 atccacggag ggtattgcaa cctcaaatca aattcctcca actacggagg atggtaccag 3540 gcagaagacg cagggttcat gtacggaagt cgtaccactt ctctcgagct cagtctgaac 3600 tcctacggat gcaggtcccc cgactacgcc aattaaggat ccgtttttgt gatctgatga 3660 taagtggttg gttcgtgtct catgcacttg ggaggtgatc tatttcacct ggtgtagttt 3720 gtgtttccgt cagttggaaa aacttatccc tatcgatttc gttttcattt tctgcttttc 3780 ttttatgtac cttcgtttgg gcttgtaacg ggcctttgta tttcaactct caataataat 3840 ccaagtgcat gttaaacaat ttgtcatctg tttcggcttt gatatactac tggtgaagat 3900 gggccgtact actgcatcac aacgaaaaat aataataaga tgaaaaactt gaagtggaaa 3960 aaaaaaaaaa cttgaatgtt cactactact cattgaccat aatgtttaac atacatagct 4020 caatagtatt tttgtgaata tggcaacaca aacagtccaa aacaattgtc tcttactata 4080 ccaaaccaag ggcgccgctt gtttgccact ctttgtgtgc aatagtgtga ttaccacatc 4140 tccacattca atatattccc tgaattatct gacgattttg atggctcact gttttcccaa 4200 gtcttgaatt gtcttctgtg cgccagtcaa atgcatatgt gttgagttta tcttttaaat 4260 atcaagcttt tgtttttaac ttttgtttgt aaccaaaaac tcacagtagg agtttgatca 4320 cataatttta tgtttgcctt tgcaatttct agtgagtctt tgattaaaag cttgaaaaga 4380 aaatgcagcc aagcttacca agtaagttat gtgtattaac cagaggaaga gagaatcttg 4440 caaaatttca acaaacacaa aaagaagtat tactacgatt ggtggagaaa gaaaacgatt 4500 ccaaatcttg aactgttgtt gtaaaagcat agcagaaagt gggagacaac cgaaatagaa 4560 atgactataa cttaatttaa tgttatcatt ataatttctt ctagcaaata tttagaaagt 4620 aaatatcaca tcaaccttta atgtaattaa gctttctctt tttgattcat gtgagatgaa 4680 aagaaaaaaa agaagagaaa agtgtagaaa acacatcatt tctaagctga aggcggccgc 4740 ggtcacccca tacgattgga agcttcccgg gcacccagct ttcttgtaca aagtgggcat 4800 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa 4860 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc 4920 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa 4980 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt 5040 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga 5100 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg 5160 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc 5220 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc 5280 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc 5340 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga 5400 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa 5460 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga 5520 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat 5580 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga 5640 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat 5700 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc 5760 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca 5820 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg 5880 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt 5940 gagttacgcg tcgttccact gagcgtcaga c 5971 <210> 121 <211> 5941 <212> DNA <213> Artificial Sequence <220> <223> plasmid UBILJWUS <400> 121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 60 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 120 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 180 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 240 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 300 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 360 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 420 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 480 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 540 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 600 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 660 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 720 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 780 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 840 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 900 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 960 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 1020 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 1080 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 1140 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 1200 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta 1260 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg 1320 ccaagtttgt acaaaaaagc aggctccggc cagaatccgg taagtgacta gggtcacatc 1380 gaattcccat aggattgggg ccagaatggc cgcggccgca tcaggatatt cttgtttaag 1440 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc 1500 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat 1560 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa 1620 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc 1680 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa 1740 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat 1800 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg 1860 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag 1920 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag 1980 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc 2040 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc 2100 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca 2160 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt 2220 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct 2280 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa 2340 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca 2400 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat 2460 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta 2520 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt 2580 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga 2640 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat 2700 taatctgagt ttttctgatt aacagccatg gaacaacctc cccaacagca gcaacaaaac 2760 gaagacggag gcggatcaag tgggggaaaa ggagggttcc tcagtaggca gtccagtact 2820 aggtggacac ccaccacaga ccagattcgc atcctcaagg agctctacta caacaacgga 2880 atacgctcac cctccgcaga acagatccag aggatctccg caagactcag acagtacggc 2940 aagatcgagg gcaagaacgt cttctactgg ttccaaaacc acaaagcccg cgaaaggcag 3000 aagaagcgct tcaccagtga gacacacttg ctgcccaacc caattcagca gcaacagcgc 3060 aacggaacaa acgcagcttg gaagcagcca gaggagcaac ccatcatcaa ccacaccaag 3120 tactcctcca acaacatatc cgcacccgca gggattatca caagtgcccc atcttcttcc 3180 gcagaaatag tctccgtggg gcagatcgga aacttcggat acggatctct gccaatggag 3240 aagtccttca gggactgcag tatctcagcc ggaggtaaca caggatacgc cgggtctgct 3300 attaaccaca acctcggatg gatgggagtc gacccatact cctctgctta caccaacttc 3360 ttcgacaaaa tacgcccaac agaggaggag actatggagg aagaaggaca ggagaacggc 3420 tcaccagaaa tcgagaccct gcctttgttc ccaatgcacg gagaggacat ccactccggc 3480 tacttcaacc tcaagagtaa ctcatcccac tacgctggcg gatggtacca aactgaggaa 3540 ggctacttca acaacggatc acgcgccagt ctggagctgt ccctgaagta ccctgacttc 3600 gcttagggat ccgtttttgt gatctgatga taagtggttg gttcgtgtct catgcacttg 3660 ggaggtgatc tatttcacct ggtgtagttt gtgtttccgt cagttggaaa aacttatccc 3720 tatcgatttc gttttcattt tctgcttttc ttttatgtac cttcgtttgg gcttgtaacg 3780 ggcctttgta tttcaactct caataataat ccaagtgcat gttaaacaat ttgtcatctg 3840 tttcggcttt gatatactac tggtgaagat gggccgtact actgcatcac aacgaaaaat 3900 aataataaga tgaaaaactt gaagtggaaa aaaaaaaaaa cttgaatgtt cactactact 3960 cattgaccat aatgtttaac atacatagct caatagtatt tttgtgaata tggcaacaca 4020 aacagtccaa aacaattgtc tcttactata ccaaaccaag ggcgccgctt gtttgccact 4080 ctttgtgtgc aatagtgtga ttaccacatc tccacattca atatattccc tgaattatct 4140 gacgattttg atggctcact gttttcccaa gtcttgaatt gtcttctgtg cgccagtcaa 4200 atgcatatgt gttgagttta tcttttaaat atcaagcttt tgtttttaac ttttgtttgt 4260 aaccaaaaac tcacagtagg agtttgatca cataatttta tgtttgcctt tgcaatttct 4320 agtgagtctt tgattaaaag cttgaaaaga aaatgcagcc aagcttacca agtaagttat 4380 gtgtattaac cagaggaaga gagaatcttg caaaatttca acaaacacaa aaagaagtat 4440 tactacgatt ggtggagaaa gaaaacgatt ccaaatcttg aactgttgtt gtaaaagcat 4500 agcagaaagt gggagacaac cgaaatagaa atgactataa cttaatttaa tgttatcatt 4560 ataatttctt ctagcaaata tttagaaagt aaatatcaca tcaaccttta atgtaattaa 4620 gctttctctt tttgattcat gtgagatgaa aagaaaaaaa agaagagaaa agtgtagaaa 4680 acacatcatt tctaagctga aggcggccgc ggtcacccca tacgattgga agcttcccgg 4740 gcacccagct ttcttgtaca aagtgggcat tataagaaag cattgcttat caatttgttg 4800 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc 4860 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca 4920 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc 4980 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag 5040 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 5100 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt 5160 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 5220 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 5280 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga 5340 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 5400 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 5460 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 5520 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 5580 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 5640 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 5700 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 5760 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 5820 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg 5880 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga 5940 c 5941 <210> 122 <211> 5932 <212> DNA <213> Artificial Sequence <220> <223> plasmid UBIPVWUS <400> 122 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 60 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 120 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 180 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 240 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 300 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 360 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 420 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 480 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 540 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 600 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 660 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 720 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 780 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 840 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 900 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 960 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 1020 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 1080 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 1140 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 1200 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta 1260 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg 1320 ccaagtttgt acaaaaaagc aggctccggc cagaatccgg taagtgacta gggtcacatc 1380 gaattcccat aggattgggg ccagaatggc cgcggccgca tcaggatatt cttgtttaag 1440 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc 1500 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat 1560 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa 1620 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc 1680 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa 1740 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat 1800 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg 1860 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag 1920 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag 1980 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc 2040 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc 2100 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca 2160 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt 2220 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct 2280 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa 2340 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca 2400 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat 2460 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta 2520 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt 2580 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga 2640 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat 2700 taatctgagt ttttctgatt aacagccatg gaaccacagc agccccaggg cagtcaacca 2760 aacgaggacg gtggaagtgg gaaaggcgga tttctctcca gacagtcctc cactaggtgg 2820 actccaacca ctgaccagat cagaatactc aaagacctct actacaacaa cggaatcaga 2880 tccccttccg cagaacagat ccaaaggatc tccgctaggc tcagacagta cgggaagatc 2940 gagggcaaga acgtcttcta ctggttccaa aaccacaagg ccagggagag acagaagaag 3000 aggttcacct ccgacaacgt ccccatgcaa agacctgcac caacaaatgc tgcacctcct 3060 tggaaacccg accaagaccc cattcacacc aagtactcca acatctcctc cacaggaatc 3120 tccagtgcat cctcctcctc agtcgagatg atcaccgtcg gacaaatggg gaactacggg 3180 tacggaagtg tgccaatgga aaagtccttc agagactgct ccatatccgc cggaggatct 3240 tccggtcatg ttggaataaa ccacaacctc ggctgggtgg gagtcgatcc ttactcttct 3300 gcatacgcca acttcttcga taaaataaga ccaaacgaag agaccctcga ggaagaagaa 3360 gaggaggagg aggaggaaga agaagaggac ggaggagcag aaattgagac cctccccctg 3420 ttcccaatgc acggagagga cattcacggc tactgcaacc tcaagtccaa cagttacaac 3480 tacgacggca acggctggta tcacagtgag gatggcttca aaaacggcag tagggcctcc 3540 ctcgagctct ccctcaactc ttacaccaga agatcccccg acttcgcata gatgcataga 3600 tccgtttttg tgatctgatg ataagtggtt ggttcgtgtc tcatgcactt gggaggtgat 3660 ctatttcacc tggtgtagtt tgtgtttccg tcagttggaa aaacttatcc ctatcgattt 3720 cgttttcatt ttctgctttt cttttatgta ccttcgtttg ggcttgtaac gggcctttgt 3780 atttcaactc tcaataataa tccaagtgca tgttaaacaa tttgtcatct gtttcggctt 3840 tgatatacta ctggtgaaga tgggccgtac tactgcatca caacgaaaaa taataataag 3900 atgaaaaact tgaagtggaa aaaaaaaaaa acttgaatgt tcactactac tcattgacca 3960 taatgtttaa catacatagc tcaatagtat ttttgtgaat atggcaacac aaacagtcca 4020 aaacaattgt ctcttactat accaaaccaa gggcgccgct tgtttgccac tctttgtgtg 4080 caatagtgtg attaccacat ctccacattc aatatattcc ctgaattatc tgacgatttt 4140 gatggctcac tgttttccca agtcttgaat tgtcttctgt gcgccagtca aatgcatatg 4200 tgttgagttt atcttttaaa tatcaagctt ttgtttttaa cttttgtttg taaccaaaaa 4260 ctcacagtag gagtttgatc acataatttt atgtttgcct ttgcaatttc tagtgagtct 4320 ttgattaaaa gcttgaaaag aaaatgcagc caagcttacc aagtaagtta tgtgtattaa 4380 ccagaggaag agagaatctt gcaaaatttc aacaaacaca aaaagaagta ttactacgat 4440 tggtggagaa agaaaacgat tccaaatctt gaactgttgt tgtaaaagca tagcagaaag 4500 tgggagacaa ccgaaataga aatgactata acttaattta atgttatcat tataatttct 4560 tctagcaaat atttagaaag taaatatcac atcaaccttt aatgtaatta agctttctct 4620 ttttgattca tgtgagatga aaagaaaaaa aagaagagaa aagtgtagaa aacacatcat 4680 ttctaagctg aaggcggccg cggtcacccc atacgattgg aagcttcccg ggcacccagc 4740 tttcttgtac aaagtgggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca 4800 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga 4860 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg 4920 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 4980 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt 5040 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 5100 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 5160 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 5220 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 5280 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 5340 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 5400 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 5460 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 5520 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 5580 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 5640 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 5700 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 5760 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 5820 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc 5880 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag ac 5932 <210> 123 <211> 5992 <212> DNA <213> Artificial Sequence <220> <223> plasmid UBIPYWUS <400> 123 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 60 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 120 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 180 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 240 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 300 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 360 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 420 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 480 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 540 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 600 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 660 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 720 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 780 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 840 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 900 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca 960 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc 1020 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga 1080 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc 1140 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc 1200 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta 1260 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg 1320 ccaagtttgt acaaaaaagc aggctccggc cagaatccgg taagtgacta gggtcacatc 1380 gaattcccat aggattgggg ccagaatggc cgcggccgca tcaggatatt cttgtttaag 1440 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc 1500 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat 1560 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa 1620 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc 1680 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa 1740 taatcataca aaaatatcca ataacactaa aaaattaaaa gaaatggata atttcacaat 1800 atgttatacg ataaagaagt tacttttcca agaaattcac tgattttata agcccacttg 1860 cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa taaagcacga agaattctag 1920 aaaatacgaa atacgcttca atgcagtggg acccacggtt caattattgc caattttcag 1980 ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa aaatataaat cgtaacgatc 2040 gttaaatctc aacggctgga tcttatgacg accgttagaa attgtggttg tcgacgagtc 2100 agtaataaac ggcgtcaaag tggttgcagc cggcacacac gagtcgtgtt tatcaactca 2160 aagcacaaat acttttcctc aacctaaaaa taaggcaatt agccaaaaac aactttgcgt 2220 gtaaacaacg ctcaatacac gtgtcatttt attattagct attgcttcac cgccttagct 2280 ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct tctataaaac aatacccaaa 2340 gagctcttct tcttcacaat tcagatttca atttctcaaa atcttaaaaa ctttctctca 2400 attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt attctctcaa aatcttcgat 2460 tttgttttcg ttcgatccca atttcgtata tgttctttgg tttagattct gttaatctta 2520 gatcgaagac gattttctgg gtttgatcgt tagatatcat cttaattctc gattagggtt 2580 tcatagatat catccgattt gttcaaataa tttgagtttt gtcgaataat tactcttcga 2640 tttgtgattt ctatctagat ctggtgttag tttctagttt gtgcgatcga atttgtcgat 2700 taatctgagt ttttctgatt aacagccatg gaaacagcac agcaccagca gaacaaccaa 2760 cagcactacc tccaccaaca cctctccatc ggacaaggaa caaacatcga ggacggcagt 2820 aacaagaaca actcctccaa cttcatgtgc aggcaaaata gtacccgctg gacaccaacc 2880 actgaccaga tcaggatatt aaaggacctc tactacaaca acggagtcag atcacctacc 2940 gctgaacaga tccagagaat ctccgccaag ctcaggcagt acggcaagat cgagggcaag 3000 aacgtcttct actggttcca gaaccataaa gcccgcgaga gacaaaagaa gcgcctcatc 3060 gccgcagcca ccaccgataa cactaacctc ccaatgcaga tgcagttcca gagaggagtc 3120 tggagatcct ctgcagacga ccccatccac cacaagtata ctaatccagg agtgcactgc 3180 ccttcagctt cttcccacgg agttctcgca gtcggacaga acggcaatca cggatacgga 3240 gccttggcaa tggaaaaatc cttccgcgac tgctccatca gtccaggcag ttccatgtca 3300 caccaccacc accaaaactt cgcttgggca ggagtcgatc cttattcctc aaccacaacc 3360 taccctttcc tcgagaagac caagcacttc gagaatgaaa ccctcgaagc tgatgaagag 3420 cagcaagagg aggaccagga gaactactac taccaaagga ccacctctgc catcgaaacc 3480 ctgcctctct tccctatgca cgaggagaac atctcctcct tctgcaacct caagcaccag 3540 gagtcatccg gaggattcta caccgagtgg tacagagcag acgacaacct cgcagcagcc 3600 agagcttccc tcgagctctc cttgaactcc ttcatcggca actcctccta gcagctgaga 3660 tccgtttttg tgatctgatg ataagtggtt ggttcgtgtc tcatgcactt gggaggtgat 3720 ctatttcacc tggtgtagtt tgtgtttccg tcagttggaa aaacttatcc ctatcgattt 3780 cgttttcatt ttctgctttt cttttatgta ccttcgtttg ggcttgtaac gggcctttgt 3840 atttcaactc tcaataataa tccaagtgca tgttaaacaa tttgtcatct gtttcggctt 3900 tgatatacta ctggtgaaga tgggccgtac tactgcatca caacgaaaaa taataataag 3960 atgaaaaact tgaagtggaa aaaaaaaaaa acttgaatgt tcactactac tcattgacca 4020 taatgtttaa catacatagc tcaatagtat ttttgtgaat atggcaacac aaacagtcca 4080 aaacaattgt ctcttactat accaaaccaa gggcgccgct tgtttgccac tctttgtgtg 4140 caatagtgtg attaccacat ctccacattc aatatattcc ctgaattatc tgacgatttt 4200 gatggctcac tgttttccca agtcttgaat tgtcttctgt gcgccagtca aatgcatatg 4260 tgttgagttt atcttttaaa tatcaagctt ttgtttttaa cttttgtttg taaccaaaaa 4320 ctcacagtag gagtttgatc acataatttt atgtttgcct ttgcaatttc tagtgagtct 4380 ttgattaaaa gcttgaaaag aaaatgcagc caagcttacc aagtaagtta tgtgtattaa 4440 ccagaggaag agagaatctt gcaaaatttc aacaaacaca aaaagaagta ttactacgat 4500 tggtggagaa agaaaacgat tccaaatctt gaactgttgt tgtaaaagca tagcagaaag 4560 tgggagacaa ccgaaataga aatgactata acttaattta atgttatcat tataatttct 4620 tctagcaaat atttagaaag taaatatcac atcaaccttt aatgtaatta agctttctct 4680 ttttgattca tgtgagatga aaagaaaaaa aagaagagaa aagtgtagaa aacacatcat 4740 ttctaagctg aaggcggccg cggtcacccc atacgattgg aagcttcccg ggcacccagc 4800 tttcttgtac aaagtgggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca 4860 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga 4920 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg 4980 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 5040 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt 5100 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 5160 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 5220 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 5280 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 5340 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 5400 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 5460 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 5520 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 5580 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 5640 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 5700 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 5760 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 5820 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 5880 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc 5940 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag ac 5992 <210> 124 <211> 2000 <212> DNA <213> Glycine max <400> 124 acatagcccc aaaaccacta ttgaatatca attagaaatt ggaagggtta cttaaccttc 60 cattaaacat acaagtttcc ccatgataat agggatcaaa aataatgatc agggattcat 120 tccaaagaaa agtttacaaa atcaattttc gctcatttta taggagttaa acccattttt 180 aagccaaaaa cctttacgta taaacaacat tttaaaagtt atttttcaat aaaagcatct 240 tttagagttt actgttttta tggcatattc accccagtac agtttatatt gctttcgttt 300 aaacaggagt tcatgaattt ctaaaaaaaa tgtttatgat acttgtcatt tcgattgttc 360 atatggtgcc tgtcagataa ttaatgtctc cgtggtgttt aactgttaag tagttgattt 420 tttgtcatgt aatcttgtaa atgacaattc tatcgttaat caaacaatcc gtatacacac 480 acacacacgg agaaactagt gtgggtttga ccaaggaccc tgaattcttc ttctttgtat 540 aaggtcaggc aagtggtaga gttcgaatag taagttttga agtttaaagc ttgtacacaa 600 aatatatata tatatatatt gataaagaat ggatataatt gttttacaac gtgcagcgag 660 ggaacccttc aatacgcacc tctctaccaa gtctaactta aaaacacaga attatcaaac 720 acccaattta tgtgttagaa tgtcttgctt ttcgctacta ctattgtctg gtgggcatga 780 tgattttctc acaaaataat tggaatattc cacttatctc tggctgatct tttttcttac 840 atttaagaca atagagagta ttaaatttgt tataatcgta atatttatat attaaatagt 900 atttatggta tatttaaata tatatatata tagaatatta ttttatatat gcatattata 960 gttattgtta attcagcatg tgttctggtt tctgaaataa aataaaaaaa caaaaatttg 1020 aaaaatagaa actagaataa aaaaaatcaa taaaaaggac ctcacttatt attttgactg 1080 ttactaatta cggaaataag ttacttaaat gtattaaatt aatttttgaa aattaatgca 1140 ttaaagaaac ttatttaatg tattaaataa gtatttaatg catttcagga aaaagcattt 1200 aatgtattaa atacagttta gaaattaatt tttgtttcat tttttattta taaaattatg 1260 ttttcttttt acatatttgt aaaattattt ggagagatga aaaaaaatgt gtaaaaagaa 1320 agagtctacc aacaagtagt tagtccaaat agtattgaat tcaaattttt taaataaagt 1380 tttgttaaaa ttaattatga ataaaattga tagataattc taacataatt acacaaaaaa 1440 atctttaatc ataatactcc ctttagacct caaatgtaaa aaaaataaaa aaaaaatcaa 1500 ttgtgttaga ttaaaatata aacaaatttt aattaactat cacctacttt atcacctact 1560 taatgatgta ttttcaaaac atcattcgtt taattgagtt ttatttttaa taatcttatt 1620 tttttttctt atataatcaa tatagatgat attttaagga ataaattaac tttttattga 1680 aattatcaaa attaaataat attaactaat tttttgtcta atggttgagt ccagagagag 1740 taatatttgt gggtggagtt ttactggtgg atgtggacag gtgtgagcca catgttacca 1800 tgcagtgagt cagaaaaaag aagtggcagg atatccggcg ggttcacggc ttggaagttg 1860 caataaatat taaatgagga tgcagatatt cttcttctgt cggtggtatg atatatatat 1920 atatataagg ccgggcagaa aggaaaggaa atactattac atagcagaag caaagaaaat 1980 actattacta gctaatgagt 2000 <210> 125 <211> 1500 <212> DNA <213> Arabidopsis thaliana <400> 125 tttattttat atgtttaatt ttatagaatc taactagctt attttgagat taaattgttt 60 agttactttt ataacagtat aaatgtataa tgaggaccta agaatgtagt cctgtaatgt 120 tcttgctatt ctacttaatc tcatcaccaa tcaaccatca aaagaagcta gtactaataa 180 aacctgcagg tattcgaata ataattaagc tcaaacacta tactaattta tggaggatta 240 tatattcaat gaattaggaa cctcatgatg gacattattg actgatataa tgtgtatact 300 aattgtgagt atttaaaaac catacaaagc atttatatgt ccacatatat tggacacaca 360 tgcaatcaat gttcaatatg ctccacacac agaaataaaa atactctttc tgatcatatg 420 atacatcata catatactaa aaaaatctaa aatgaactat aaccacaagc atatataata 480 acaatgaaat ggtaatgttt cttcattttt atttgttcaa attcttattc ggttgttttt 540 tcttacccta cgagaatccg tgaggtcaaa gggaaacagt gatttttttt ttgtattttg 600 ttttttaaat tgatgaactg taaaactctc tctctagaaa aatatataag tagtagtatg 660 aattttctct cactaaaagc attaatggac ctttcgataa tcataaatgc aatgcaccct 720 ctctatgcat ttcgcaataa ctccttttcc ttctgccaca tcctcttcct cacctctttc 780 tcttcttccc tttctcctaa gttcctcctc caccaaattc tccatttatt tcgttaacta 840 tcctccattt gttttcttct gaagagtgat atattctacc tttctctggt taaagaaact 900 ccctgaatcc accggttatg tcttgaccgg ctataagcct ataaactgat gccctaagac 960 acctttttag gtttctcaat aattctccgc atctatcttt tcttctccac aagtaagaga 1020 accagaaaac cagagaagaa gccgagctag ctagggtttc attgtgtgca caaaagtaag 1080 atctctctct ctaaccaata cttgtgtaat ttgtctttgt ttctttgagc aaatattgca 1140 tgtttgttca tattagccgg atccgtttta tattttttca tgatctacat tttatcttta 1200 ttttgtttgt aaattaatga gttttttttt tttttttctg tttttgtcac gatctaaaaa 1260 acaagcgtta caaagaagaa gaaaaacctt tttggagtta gaagtgtaaa aggggtttca 1320 gtttgacgaa ttttccttag tagttgtgta aaaaaaggcc attgacttaa tgtcaactct 1380 atatatctac acattttttt attaattagt ttttgttttt ttcccacttc atttaccttt 1440 agtcaatgaa tttttactga aaacgttttt tcaaggtcaa tttcactgag ttaaaaaaaa 1500 <210> 126 <211> 1130 <212> DNA <213> Arabidopsis thaliana <400> 126 atatcggttg ttgacgatta accatgcaaa aaagaaacat taattgcgaa tgtaaataac 60 aaaacatgta actcttgtag atatacatgt atcgacattt aaacccgaat atatatgtat 120 acctataatt tctctgattt tcacgctacc tgccacgtac atgggtgata ggtccaaact 180 cacaagtaaa agtttacgta cagtgaattc gtctttttgg gtataaacgt acatttaatt 240 tacacgtaag aaaggattac caattctttc atttatggta ccagacagag ttaaggcaaa 300 caagagaaac atatagagtt ttgatatgtt ttcttggata aatattaaat tgatgcaata 360 tttagggatg gacacaaggt aatatatgcc ttttaaggta tatgtgctat atgaatcgtt 420 tcgcatgggt actaaaatta tttgtcctta ctttatataa acaaattcca acaaaatcaa 480 gtttttgcta aaactagttt atttgcgggt tatttaatta cctatcatat tacttgtaat 540 atcattcgta tgttaacggg taaaccaaac caaaccggat attgaactat taaaaatctt 600 gtaaatttga cacaaactaa tgaatatcta aattatgtta ctgctatgat aacgaccatt 660 tttgtttttg agaaccataa tataaattac aggtacgtga caagtactaa gtatttatat 720 ccacctttag tcacagtacc aatattgcgc ctaccgggca acgtgaacgt gatcatcaaa 780 tcaaagtagt taccaaacgc tttgatctcg ataaaactaa aagctgacac gtcttgctgt 840 ttcttaattt atttctctta caacgacaat tttgagaaat atgaaatttt tatatcgaaa 900 gggaacagtc cttatcattt gctcccatca cttgcttttg tctagttaca actggaaatc 960 gaagagaagt attacaaaaa catttttctc gtcatttata aaaaaatgac aaaaaattaa 1020 atagagagca aagcaagagc gttgggtgac gttggtctct tcattaactc ctctcatcta 1080 ccccttcctc tgttcgcctt tatatccttc accttccctc tctcatcttc 1130 <210> 127 <211> 663 <212> DNA <213> Gnetum gnemon <400> 127 atggaagaaa acagcaacag cagcagaagc agcaacagcg gcgcacgttg gaatccgacg 60 ccggagcagc tgagcattct gaaagagctg tatcatggcc gcggaatcag gtcgcccagc 120 gccgaacaga tccaccatat ctcatggaaa ctcagctcct acggcaaaat cgagggcaag 180 aacgtcttct actggttcca gaaccataag gcccgccagc gtcaaaaaga gcgtctcggt 240 tccgtcatgc ctgcttctgc ttcttcttct tcttttgcat ctccttctca gggagctgtc 300 ggcattcgaa ggggaagtac ggtgctatgt cttaaggaaa cttcagcttc atcttcatct 360 tattcttctg ttccgcgttc ggtatcagct gtgcgccagt gtgctgcatc tcgtgactgt 420 ggcttgcacc atgatctgac aactctggag ctgtttcccc tgcaccgtga aagtagaata 480 gacgctgggg aggaagacga ggagcaacag gaggtggtgg tgctagataa taataataac 540 acctggacaa ggcaccaaaa tgtaaatgtg aatcatgcaa ataatgacga tgagccacag 600 catggtcatg ggcagattga tattgatgta gggcttcgtc tttgcctcgg tggatttcgc 660 tga 663 <210> 128 <211> 220 <212> PRT <213> Gnetum gnemon <400> 128 Met Glu Glu Asn Ser Asn Ser Ser Arg Ser Ser Asn Ser Gly Ala Arg 1 5 10 15 Trp Asn Pro Thr Pro Glu Gln Leu Ser Ile Leu Lys Glu Leu Tyr His 20 25 30 Gly Arg Gly Ile Arg Ser Pro Ser Ala Glu Gln Ile His His Ile Ser 35 40 45 Trp Lys Leu Ser Ser Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr 50 55 60 Trp Phe Gln Asn His Lys Ala Arg Gln Arg Gln Lys Glu Arg Leu Gly 65 70 75 80 Ser Val Met Pro Ala Ser Ala Ser Ser Ser Ser Phe Ala Ser Pro Ser 85 90 95 Gln Gly Ala Val Gly Ile Arg Arg Gly Ser Thr Val Leu Cys Leu Lys 100 105 110 Glu Thr Ser Ala Ser Ser Ser Ser Tyr Ser Ser Val Pro Arg Ser Val 115 120 125 Ser Ala Val Arg Gln Cys Ala Ala Ser Arg Asp Cys Gly Leu His His 130 135 140 Asp Leu Thr Thr Leu Glu Leu Phe Pro Leu His Arg Glu Ser Arg Ile 145 150 155 160 Asp Ala Gly Glu Glu Asp Glu Glu Gln Gln Glu Val Val Val Leu Asp 165 170 175 Asn Asn Asn Asn Thr Trp Thr Arg His Gln Asn Val Asn Val Asn His 180 185 190 Ala Asn Asn Asp Asp Glu Pro Gln His Gly His Gly Gln Ile Asp Ile 195 200 205 Asp Val Gly Leu Arg Leu Cys Leu Gly Gly Phe Arg 210 215 220 <210> 129 <211> 987 <212> DNA <213> Malus domestica <400> 129 atggaccctc aacagaaccc aaatgaagta caagaaggag gcaacaggca ggggggtgga 60 atcatgtgca ggcaaagcag tacgcggtgg acacccacaa ctgatcagat aaaaatcttg 120 aaggaccttt actacaacaa tggcattagg tcacccagcg ctgagcagat tcagaggatc 180 tctgctaagc tgcgacagtt cggcaagatc gaaggcaaga acgttttcta ttggtttcag 240 aaccacaaag ctcgtgagag gcagaagaaa agattcactt cttcttctgc tcctgatcac 300 catcatcatg cagcaccacc agtgccagct gtaccaccag aatccaataa tatcaggcaa 360 agatcatcag gggttgggat tgatattaat gcaactgctg ctgctgctta tgaacaagca 420 cccattaatc accaccgcaa gtattccaac atttctgctc caccagccgg gttttcttct 480 gcatcttctt cttcagttgg tgtgaatgtt tctgttgggg cacagatggg aaactatggg 540 tatggatcca ttgctatgga gaagagcttt agggagtgct caatatcatc tggaggaagt 600 actagcactg gtcatgtggg tggatctaat tataatactt ttaatcacaa ctatggatca 660 tgggttggtg ttgatccata ttcctcgccc tacactatct ttgacaagaa atcctcatca 720 aaaccagtgt ttggtgacca ggaaaatatg acggaagaag aatactacaa tctgcaagct 780 tcccaagaga ttgagactct cccactcttc cccatgcacg gtgaagacat tcatggcttt 840 ggcaacatca agtcatcctc tatggatggc tactactccg gctggtacag gtccgatggc 900 ggcaacgatg gtggctctcg cacttccctt gagcttagcc tcaattccta cggtcacatg 960 acccatgatt acttcagatc atattga 987 <210> 130 <211> 328 <212> PRT <213> Malus domestica <400> 130 Met Asp Pro Gln Gln Asn Pro Asn Glu Val Gln Glu Gly Gly Asn Arg 1 5 10 15 Gln Gly Gly Gly Ile Met Cys Arg Gln Ser Ser Thr Arg Trp Thr Pro 20 25 30 Thr Thr Asp Gln Ile Lys Ile Leu Lys Asp Leu Tyr Tyr Asn Asn Gly 35 40 45 Ile Arg Ser Pro Ser Ala Glu Gln Ile Gln Arg Ile Ser Ala Lys Leu 50 55 60 Arg Gln Phe Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln 65 70 75 80 Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Phe Thr Ser Ser Ser 85 90 95 Ala Pro Asp His His His His Ala Ala Pro Pro Val Pro Ala Val Pro 100 105 110 Pro Glu Ser Asn Asn Ile Arg Gln Arg Ser Ser Gly Val Gly Ile Asp 115 120 125 Ile Asn Ala Thr Ala Ala Ala Ala Tyr Glu Gln Ala Pro Ile Asn His 130 135 140 His Arg Lys Tyr Ser Asn Ile Ser Ala Pro Pro Ala Gly Phe Ser Ser 145 150 155 160 Ala Ser Ser Ser Ser Val Gly Val Asn Val Ser Val Gly Ala Gln Met 165 170 175 Gly Asn Tyr Gly Tyr Gly Ser Ile Ala Met Glu Lys Ser Phe Arg Glu 180 185 190 Cys Ser Ile Ser Ser Gly Gly Ser Thr Ser Thr Gly His Val Gly Gly 195 200 205 Ser Asn Tyr Asn Thr Phe Asn His Asn Tyr Gly Ser Trp Val Gly Val 210 215 220 Asp Pro Tyr Ser Ser Pro Tyr Thr Ile Phe Asp Lys Lys Ser Ser Ser 225 230 235 240 Lys Pro Val Phe Gly Asp Gln Glu Asn Met Thr Glu Glu Glu Tyr Tyr 245 250 255 Asn Leu Gln Ala Ser Gln Glu Ile Glu Thr Leu Pro Leu Phe Pro Met 260 265 270 His Gly Glu Asp Ile His Gly Phe Gly Asn Ile Lys Ser Ser Ser Met 275 280 285 Asp Gly Tyr Tyr Ser Gly Trp Tyr Arg Ser Asp Gly Gly Asn Asp Gly 290 295 300 Gly Ser Arg Thr Ser Leu Glu Leu Ser Leu Asn Ser Tyr Gly His Met 305 310 315 320 Thr His Asp Tyr Phe Arg Ser Tyr 325 <210> 131 <211> 867 <212> DNA <213> Manihot esculenta <400> 131 atggaacctc aacaacaaca gcagcagcag caaaaccaac aacaaccaaa cgatgacaat 60 agcagcggtg ccaaaggaaa ctttctttgc aggcaaagca gtactaggtg gactcccaca 120 actgaccaaa taagaatatt gaaggacctt tactacaaca gtggagttag gtccccaagt 180 gcagagcaga ttcagaggat atctgctagg cttagacagt acggtaagat tgaaggcaag 240 aatgtctttt attggtttca gaaccataaa gctcgtgaga ggcagaagaa aaggttcacc 300 actgatgtcc ccatgcaaca aagaactgtt tcgaatgctt ctagttggaa gcctgaagac 360 tattccttcc acaacaaata ccccaacatt gctcctgggt tttcttctgc atctccatcc 420 tcagctgctg ggcttcctgt tggacagatg ggaaactatg ggtatggatc tgtaactatg 480 gagaagagtt ttagggactg ctcaatatca gctactgcca acggtggtgt tggtggatcc 540 atgagcccca actgtgggtg ggttggcatt gatccctact gttcatccta ctctctcttt 600 gacaagcaaa aatcaactaa tgaaacccta gaagatgaag aacaagatca agatcaagaa 660 gaggaagcaa ctccagggat tgaaaccctc cctctcttcc ctacccagag agaagacatc 720 aatggcttct gcaacatgaa gcaccacccc atctgctact ccgaaaacta ctgctatggc 780 tctgacgatg gaaacaacgc ttcgcgtact tccctcgaac ttagcctcaa ctcttacagc 840 aatgggcagg cacgggattc catctaa 867 <210> 132 <211> 288 <212> PRT <213> Manihot esculenta <400> 132 Met Glu Pro Gln Gln Gln Gln Gln Gln Gln Gln Asn Gln Gln Gln Pro 1 5 10 15 Asn Asp Asp Asn Ser Ser Gly Ala Lys Gly Asn Phe Leu Cys Arg Gln 20 25 30 Ser Ser Thr Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys 35 40 45 Asp Leu Tyr Tyr Asn Ser Gly Val Arg Ser Pro Ser Ala Glu Gln Ile 50 55 60 Gln Arg Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys 65 70 75 80 Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys 85 90 95 Lys Arg Phe Thr Thr Asp Val Pro Met Gln Gln Arg Thr Val Ser Asn 100 105 110 Ala Ser Ser Trp Lys Pro Glu Asp Tyr Ser Phe His Asn Lys Tyr Pro 115 120 125 Asn Ile Ala Pro Gly Phe Ser Ser Ala Ser Pro Ser Ser Ala Ala Gly 130 135 140 Leu Pro Val Gly Gln Met Gly Asn Tyr Gly Tyr Gly Ser Val Thr Met 145 150 155 160 Glu Lys Ser Phe Arg Asp Cys Ser Ile Ser Ala Thr Ala Asn Gly Gly 165 170 175 Val Gly Gly Ser Met Ser Pro Asn Cys Gly Trp Val Gly Ile Asp Pro 180 185 190 Tyr Cys Ser Ser Tyr Ser Leu Phe Asp Lys Gln Lys Ser Thr Asn Glu 195 200 205 Thr Leu Glu Asp Glu Glu Gln Asp Gln Asp Gln Glu Glu Glu Ala Thr 210 215 220 Pro Gly Ile Glu Thr Leu Pro Leu Phe Pro Thr Gln Arg Glu Asp Ile 225 230 235 240 Asn Gly Phe Cys Asn Met Lys His His Pro Ile Cys Tyr Ser Glu Asn 245 250 255 Tyr Cys Tyr Gly Ser Asp Asp Gly Asn Asn Ala Ser Arg Thr Ser Leu 260 265 270 Glu Leu Ser Leu Asn Ser Tyr Ser Asn Gly Gln Ala Arg Asp Ser Ile 275 280 285 <210> 133 <211> 960 <212> DNA <213> Kalanchoe fedtschenkoi <400> 133 atggaagctc aacagcaaca gcataataca atgatgggtg atgaccttgg cggtggcaag 60 ttggcagcca ccagcttcac aagagcgcaa accaacagca gcaacaacca cagcctagtt 120 aataacaacg gcggcagaag aagcagcacg aggtggacac caacaacaga ccagataaga 180 atactcaaag atctttacta caacaatgga gtgaggtctc ccacggtgga gcagattcag 240 aggatctctg ctaggttgag acagtacggt aagatcgagg gcaaaaatgt cttctactgg 300 tttcagaacc aaaaggctcg tgaaagacag aagaggaggt tcaacacaac cagcaccacc 360 aacaacaaca ctgctcccgc tcctctccct ccatcttcta ctgtttgtaa accttcttcg 420 ctagactcat ccactccaca cattcacact gcagcagcag ctgctgctgc tgcctccaaa 480 tactcagaca tcaacaacaa ccaaaacctt atctacccag ctgggcacaa tcagatgtca 540 ggtttcggat atgctgcctc cgtaacaatg gagaagagct tcagagattg ttctatatcg 600 acgggcgggt catctttcgg ttgggctaaa ggaggaatga cagatcacca tctgtctcgt 660 catccctacc cgcacacttc accctatgca atctacaacc agcggaaatc aataaacagt 720 gatgagtatc acgagctact ggcatcatca acttcggatc atcatgaaca tggggataat 780 cggggtccgg agattgaaac cctgccacta tttcccatgc acggcgagga catcaactac 840 ggcttctgcc accttaattc agccgagact gatggctggt tcaatcgcga tgtgggtagt 900 ggctctcgtg cgtccctcga gctcagtctc aactcttacg caggaatgga ccacacctag 960 <210> 134 <211> 319 <212> PRT <213> Kalanchoe fedtschenkoi <400> 134 Met Glu Ala Gln Gln Gln Gln His Asn Thr Met Met Gly Asp Asp Leu 1 5 10 15 Gly Gly Gly Lys Leu Ala Ala Thr Ser Phe Thr Arg Ala Gln Thr Asn 20 25 30 Ser Ser Asn Asn His Ser Leu Val Asn Asn Asn Gly Gly Arg Arg Ser 35 40 45 Ser Thr Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp 50 55 60 Leu Tyr Tyr Asn Asn Gly Val Arg Ser Pro Thr Val Glu Gln Ile Gln 65 70 75 80 Arg Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn 85 90 95 Val Phe Tyr Trp Phe Gln Asn Gln Lys Ala Arg Glu Arg Gln Lys Arg 100 105 110 Arg Phe Asn Thr Thr Ser Thr Thr Asn Asn Asn Thr Ala Pro Ala Pro 115 120 125 Leu Pro Pro Ser Ser Thr Val Cys Lys Pro Ser Ser Leu Asp Ser Ser 130 135 140 Thr Pro His Ile His Thr Ala Ala Ala Ala Ala Ala Ala Ala Ser Lys 145 150 155 160 Tyr Ser Asp Ile Asn Asn Asn Gln Asn Leu Ile Tyr Pro Ala Gly His 165 170 175 Asn Gln Met Ser Gly Phe Gly Tyr Ala Ala Ser Val Thr Met Glu Lys 180 185 190 Ser Phe Arg Asp Cys Ser Ile Ser Thr Gly Gly Ser Ser Phe Gly Trp 195 200 205 Ala Lys Gly Gly Met Thr Asp His His Leu Ser Arg His Pro Tyr Pro 210 215 220 His Thr Ser Pro Tyr Ala Ile Tyr Asn Gln Arg Lys Ser Ile Asn Ser 225 230 235 240 Asp Glu Tyr His Glu Leu Leu Ala Ser Ser Thr Ser Asp His His Glu 245 250 255 His Gly Asp Asn Arg Gly Pro Glu Ile Glu Thr Leu Pro Leu Phe Pro 260 265 270 Met His Gly Glu Asp Ile Asn Tyr Gly Phe Cys His Leu Asn Ser Ala 275 280 285 Glu Thr Asp Gly Trp Phe Asn Arg Asp Val Gly Ser Gly Ser Arg Ala 290 295 300 Ser Leu Glu Leu Ser Leu Asn Ser Tyr Ala Gly Met Asp His Thr 305 310 315 <210> 135 <211> 840 <212> DNA <213> Gossypium hirsutum <400> 135 atggaacccc agcaacagca acagcaaaac caacaagctc caaatgagga ttgtggtggt 60 ggcagtggca aaggaagctt tctttgcagg caaagtagta cacggtggac tcctaccact 120 gaccagatta gaatattgaa ggacctttac tacaacaatg gagttaggtc cccgagtgct 180 gatcagattc aaaggatctc tgctagactt agacagtacg gaaaaatcga aggcaagaat 240 gtcttttatt ggtttcagaa ccacaaggct cgtgagaggc agaagaaaag gttcaccacc 300 acgactaatc atcatgtccc catgcaaagc cctaccactg ctgcaaataa caagtatccc 360 aacataactc ctggttattc tcctacatct ctttcttcaa ctggtgctct tactgttggg 420 cagataggaa actatggcta tgggtctata actatggaaa agagttttag ggattgctca 480 atatcagcat gtggtagtag tacaagtgta ggcgaatccc tgagtcacaa ctttggatgg 540 gttgggttcg atcctactta ttcttcgtcc tacacattct ttgagcacaa aaaattcatg 600 gaagagagac aaaaagatga agatgatgca gaagaagaag ctgctgctcc acaaattgag 660 accctccctc tctttcccat gcacagtgaa gacgctaatg gtttttgcag taatattagg 720 cccagactag acagctgcta ctctgggtgg tacaggtctg aagatggcta cactggttct 780 cgtgcctcac ttgagctcag cctcaaccca tatgcaggca gaccgcaaga ttctatctga 840 <210> 136 <211> 279 <212> PRT <213> Gossypium hirsutum <400> 136 Met Glu Pro Gln Gln Gln Gln Gln Gln Asn Gln Gln Ala Pro Asn Glu 1 5 10 15 Asp Cys Gly Gly Gly Ser Gly Lys Gly Ser Phe Leu Cys Arg Gln Ser 20 25 30 Ser Thr Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp 35 40 45 Leu Tyr Tyr Asn Asn Gly Val Arg Ser Pro Ser Ala Asp Gln Ile Gln 50 55 60 Arg Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn 65 70 75 80 Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys 85 90 95 Arg Phe Thr Thr Thr Thr Asn His His Val Pro Met Gln Ser Pro Thr 100 105 110 Thr Ala Ala Asn Asn Lys Tyr Pro Asn Ile Thr Pro Gly Tyr Ser Pro 115 120 125 Thr Ser Leu Ser Ser Thr Gly Ala Leu Thr Val Gly Gln Ile Gly Asn 130 135 140 Tyr Gly Tyr Gly Ser Ile Thr Met Glu Lys Ser Phe Arg Asp Cys Ser 145 150 155 160 Ile Ser Ala Cys Gly Ser Ser Thr Ser Val Gly Glu Ser Leu Ser His 165 170 175 Asn Phe Gly Trp Val Gly Phe Asp Pro Thr Tyr Ser Ser Ser Tyr Thr 180 185 190 Phe Phe Glu His Lys Lys Phe Met Glu Glu Arg Gln Lys Asp Glu Asp 195 200 205 Asp Ala Glu Glu Glu Ala Ala Ala Pro Gln Ile Glu Thr Leu Pro Leu 210 215 220 Phe Pro Met His Ser Glu Asp Ala Asn Gly Phe Cys Ser Asn Ile Arg 225 230 235 240 Pro Arg Leu Asp Ser Cys Tyr Ser Gly Trp Tyr Arg Ser Glu Asp Gly 245 250 255 Tyr Thr Gly Ser Arg Ala Ser Leu Glu Leu Ser Leu Asn Pro Tyr Ala 260 265 270 Gly Arg Pro Gln Asp Ser Ile 275 <210> 137 <211> 969 <212> DNA <213> Zostera marina <400> 137 atggaacatc atcatcagca gcagttccac ggtggccagc cgcagctgat gagtgaaaat 60 gaagagatga acaacaatgg gagcgggggt gggagtagta agagcagttt tctttgcaga 120 cagtcgagca caaggtggat acccactact gatcagataa ggatactgaa ggatctatac 180 tacaacagtg gactgagatc cccaaacgcc gatcagatcc aaagaatatc cgccagactg 240 aggcagtacg gaaagatcga gggtaagaat gttttctact ggtttcaaaa tcacaaagcc 300 cgagagaggc agaagaagag actcaccgtc gatattgctg cagcggcaac taccaacaaa 360 ttctccaaca acactaatat gtcttcttct tcttctccct cagggatttc tcctgcttct 420 catgcagtgt atgcaggatt agggcagatg gggagcaatg gcggtggatc agtcgtgatg 480 gagagaactt cttacatgga gtgttccttg ccaggtggga tgccgatgcc cgtacctgaa 540 cccaatgtgg gagtcggagt gggatgggtg agtgttgaaa caggagctac tactatgcaa 600 caattctact accatcaaca gagaccaacc acaccctttc ctgctcctca tcatcaccac 660 cattactacg acgacggtta ctattccaat aataacaaac ttcaagccca tcacctacat 720 atacgcgagg aaaatcaaca tccagaagat gacgacgaag aagaagaaga agaagaagaa 780 gatgaagaga tagaaacact tccattgttc ccaatccact cacaggagac acagtcttac 840 ttcctggacc aacaacatgc aatccacaat caagaaagca ataacatgga aacctctttg 900 gagctcaccc taaactccta ctatgctgct tccagtagca gtactccaca tggatcagag 960 catgattaa 969 <210> 138 <211> 322 <212> PRT <213> Zostera marina <400> 138 Met Glu His His His Gln Gln Gln Phe His Gly Gly Gln Pro Gln Leu 1 5 10 15 Met Ser Glu Asn Glu Glu Met Asn Asn Asn Gly Ser Gly Gly Gly Ser 20 25 30 Ser Lys Ser Ser Phe Leu Cys Arg Gln Ser Ser Thr Arg Trp Ile Pro 35 40 45 Thr Thr Asp Gln Ile Arg Ile Leu Lys Asp Leu Tyr Tyr Asn Ser Gly 50 55 60 Leu Arg Ser Pro Asn Ala Asp Gln Ile Gln Arg Ile Ser Ala Arg Leu 65 70 75 80 Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln 85 90 95 Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg Leu Thr Val Asp Ile 100 105 110 Ala Ala Ala Ala Thr Thr Asn Lys Phe Ser Asn Asn Thr Asn Met Ser 115 120 125 Ser Ser Ser Ser Pro Ser Gly Ile Ser Pro Ala Ser His Ala Val Tyr 130 135 140 Ala Gly Leu Gly Gln Met Gly Ser Asn Gly Gly Gly Ser Val Val Met 145 150 155 160 Glu Arg Thr Ser Tyr Met Glu Cys Ser Leu Pro Gly Gly Met Pro Met 165 170 175 Pro Val Pro Glu Pro Asn Val Gly Val Gly Val Gly Trp Val Ser Val 180 185 190 Glu Thr Gly Ala Thr Thr Met Gln Gln Phe Tyr Tyr His Gln Gln Arg 195 200 205 Pro Thr Thr Pro Phe Pro Ala Pro His His His His His Tyr Tyr Asp 210 215 220 Asp Gly Tyr Tyr Ser Asn Asn Asn Lys Leu Gln Ala His His Leu His 225 230 235 240 Ile Arg Glu Glu Asn Gln His Pro Glu Asp Asp Asp Glu Glu Glu Glu 245 250 255 Glu Glu Glu Glu Asp Glu Glu Ile Glu Thr Leu Pro Leu Phe Pro Ile 260 265 270 His Ser Gln Glu Thr Gln Ser Tyr Phe Leu Asp Gln Gln His Ala Ile 275 280 285 His Asn Gln Glu Ser Asn Asn Met Glu Thr Ser Leu Glu Leu Thr Leu 290 295 300 Asn Ser Tyr Tyr Ala Ala Ser Ser Ser Ser Thr Pro His Gly Ser Glu 305 310 315 320 His Asp <210> 139 <211> 693 <212> DNA <213> Amborella trichopoda <400> 139 atggatcatt ctgataactg cccaggtaac aacaacaact ctggttctgg ttctggttct 60 ggaaccaagt ctggttacat gtgcagacaa tctaactcta gatggatccc aaccgctgaa 120 caaatcagaa tccttagaga actttactac tctaacggtg ttagatctcc aaccgctgaa 180 caaatccaaa gaatctctgc tagacttaga caatacggta agatcgaagg taagaacgtt 240 ttttactggt ttcaaaacca taaggctaga gaaagacaaa agaagagact taccgttgat 300 cttgctgttc aaagagctgc tgctacctct ggtggtcttc caccaggtgt tcttaacgtt 360 tctcatatcg ctggttctac ctgcggttct gttcttatgg aatcttctct tatggattgc 420 tgctctacct ctgctcttga aaaccataac ggtagaaagg aaagagctaa cggtgatatg 480 atcgttgaaa gagaaagatc tgctccaggt aaggaaaccc ttcaactttt tccaatgcat 540 cattctccac aaaacgatga taccatcgct gatgattact tttttaacga tgaagaagaa 600 aagccagttt gcatggcttc tcttgaactt acccttaact cttttgatag agtttaccat 660 ccagattctg attttagatc tcattcttct taa 693 <210> 140 <211> 230 <212> PRT <213> Amborella trichopoda <400> 140 Met Asp His Ser Asp Asn Cys Pro Gly Asn Asn Asn Asn Ser Gly Ser 1 5 10 15 Gly Ser Gly Ser Gly Thr Lys Ser Gly Tyr Met Cys Arg Gln Ser Asn 20 25 30 Ser Arg Trp Ile Pro Thr Ala Glu Gln Ile Arg Ile Leu Arg Glu Leu 35 40 45 Tyr Tyr Ser Asn Gly Val Arg Ser Pro Thr Ala Glu Gln Ile Gln Arg 50 55 60 Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile Glu Gly Lys Asn Val 65 70 75 80 Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu Arg Gln Lys Lys Arg 85 90 95 Leu Thr Val Asp Leu Ala Val Gln Arg Ala Ala Ala Thr Ser Gly Gly 100 105 110 Leu Pro Pro Gly Val Leu Asn Val Ser His Ile Ala Gly Ser Thr Cys 115 120 125 Gly Ser Val Leu Met Glu Ser Ser Leu Met Asp Cys Cys Ser Thr Ser 130 135 140 Ala Leu Glu Asn His Asn Gly Arg Lys Glu Arg Ala Asn Gly Asp Met 145 150 155 160 Ile Val Glu Arg Glu Arg Ser Ala Pro Gly Lys Glu Thr Leu Gln Leu 165 170 175 Phe Pro Met His His Ser Pro Gln Asn Asp Asp Thr Ile Ala Asp Asp 180 185 190 Tyr Phe Phe Asn Asp Glu Glu Glu Lys Pro Val Cys Met Ala Ser Leu 195 200 205 Glu Leu Thr Leu Asn Ser Phe Asp Arg Val Tyr His Pro Asp Ser Asp 210 215 220 Phe Arg Ser His Ser Ser 225 230 <210> 141 <211> 834 <212> DNA <213> Aquilegia coerulea <400> 141 atggaacctc aacaagatcc caataaccaa caacaacaac ttcaacaaca acaacatgaa 60 gaaaacagca gtggaagtag tagcaaaagc agttttcttt gcaggcaaac tagtacaagg 120 tggacaccca caagtgatca gataagaatc ttgaaagacc tttactacaa caatggagtt 180 aggtctccaa gtgcagagca aatacagaga atctcagcaa ggttaaggca gtacggtaag 240 attgaaggca agaatgtttt ctattggttt cagaaccaca aagctcgtga gagacaaaag 300 aagagattca caaatgatac aacaaccatg tccatgcaaa gagctactac tgcaactggt 360 catgccaaat acattactac ttctcctggg atttcatctc catcttctgc tggtgtactt 420 ggtgttggac agatgggaag caacatggga tatggatctg ttgttatgga gaagagtttt 480 agagactgtt caatatctcc tgctgctggt ggtggtggtg gtggtggcag taatatgggt 540 ggatatggat ccatgaacaa caattttggt tggattggtg cgggtgtcga gccatatcca 600 tttttcaaca agacaaagca tattcaaaaa tatgaagaag aagatttctc ccaaactgag 660 attgaaacac taccactatt tcccatgcat ggtgaagaca gtacaaggtt tggttcatct 720 ggcaacccaa acccgaattg gaactggttt cgaacaggag atgaaatgat tggatctaga 780 acatcattgg agcttagctt gaattcttac tctggcgggt caattaataa ctga 834 <210> 142 <211> 277 <212> PRT <213> Aquilegia coerulea <400> 142 Met Glu Pro Gln Gln Asp Pro Asn Asn Gln Gln Gln Gln Leu Gln Gln 1 5 10 15 Gln Gln His Glu Glu Asn Ser Ser Gly Ser Ser Ser Lys Ser Ser Phe 20 25 30 Leu Cys Arg Gln Thr Ser Thr Arg Trp Thr Pro Thr Ser Asp Gln Ile 35 40 45 Arg Ile Leu Lys Asp Leu Tyr Tyr Asn Asn Gly Val Arg Ser Pro Ser 50 55 60 Ala Glu Gln Ile Gln Arg Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys 65 70 75 80 Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg 85 90 95 Glu Arg Gln Lys Lys Arg Phe Thr Asn Asp Thr Thr Thr Met Ser Met 100 105 110 Gln Arg Ala Thr Thr Ala Thr Gly His Ala Lys Tyr Ile Thr Thr Ser 115 120 125 Pro Gly Ile Ser Ser Pro Ser Ser Ala Gly Val Leu Gly Val Gly Gln 130 135 140 Met Gly Ser Asn Met Gly Tyr Gly Ser Val Val Met Glu Lys Ser Phe 145 150 155 160 Arg Asp Cys Ser Ile Ser Pro Ala Ala Gly Gly Gly Gly Gly Gly Gly 165 170 175 Ser Asn Met Gly Gly Tyr Gly Ser Met Asn Asn Asn Phe Gly Trp Ile 180 185 190 Gly Ala Gly Val Glu Pro Tyr Pro Phe Phe Asn Lys Thr Lys His Ile 195 200 205 Gln Lys Tyr Glu Glu Glu Asp Phe Ser Gln Thr Glu Ile Glu Thr Leu 210 215 220 Pro Leu Phe Pro Met His Gly Glu Asp Ser Thr Arg Phe Gly Ser Ser 225 230 235 240 Gly Asn Pro Asn Pro Asn Trp Asn Trp Phe Arg Thr Gly Asp Glu Met 245 250 255 Ile Gly Ser Arg Thr Ser Leu Glu Leu Ser Leu Asn Ser Tyr Ser Gly 260 265 270 Gly Ser Ile Asn Asn 275 <210> 143 <211> 969 <212> DNA <213> Amaranthus hypochondriacus <400> 143 atggagtttc aacaacaaca agatgaagag atgataaacc atgttgggaa aatgagcaac 60 aacacaagta acaataataa taataataat aactatttat gtagacaaag tagcacaaga 120 tggactccaa caacagaaca aataaagctt ttaaaagatc tttattattt acaaggaatt 180 agatctccta ctgctgatca aattcaaagc atttgtgcta ggttacgtcg ttatggaaaa 240 attgaaggca aaaatgtttt ttattggttt caaaatcata aggctaggga aagacaaaaa 300 aagaagctta ctcctacttc tcctaataat aataataata ataataataa taatttcata 360 agaaataata ataataataa taataatgga aatattaaca cttttcatgc taaatatcct 420 tgttccttca ataataatcc cggggtttct tgttcaagtt caccagcagg tgtgatttct 480 ggaggtgggc aattgatggg gagttttggg tatggagctg tgactatgga gactaaattt 540 agggtaagag gaggtattcc tattccaaat ggagcaaatc aatggagtaa ctatttcaac 600 ccaaacaatc caacatctta ctcaaatttc tttattgaca aggtatcatc aaatgttgat 660 catcattatc catacattaa ccatcatgat catgatcatg atcatgatca tcatgatcat 720 gatcattatg gcccacaatc acctcatgag attcagactc ttcctctctt tcccatgcac 780 aatgatcatc atgatctcga tctcgatctc gatctcgatc acgatcaagg gttctacacc 840 tataagcctc aatcggacgg ctgtaattat accggaggat atcgatggtc atctaatgtt 900 aatggtggac atacttctct tgagcttagt cttaactctt atggatttgg atgctattca 960 cctaattaa 969 <210> 144 <211> 322 <212> PRT <213> Amaranthus hypochondriacus <400> 144 Met Glu Phe Gln Gln Gln Gln Asp Glu Glu Met Ile Asn His Val Gly 1 5 10 15 Lys Met Ser Asn Asn Thr Ser Asn Asn Asn Asn Asn Asn Asn Asn Tyr 20 25 30 Leu Cys Arg Gln Ser Ser Thr Arg Trp Thr Pro Thr Thr Glu Gln Ile 35 40 45 Lys Leu Leu Lys Asp Leu Tyr Tyr Leu Gln Gly Ile Arg Ser Pro Thr 50 55 60 Ala Asp Gln Ile Gln Ser Ile Cys Ala Arg Leu Arg Arg Tyr Gly Lys 65 70 75 80 Ile Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg 85 90 95 Glu Arg Gln Lys Lys Lys Leu Thr Pro Thr Ser Pro Asn Asn Asn Asn 100 105 110 Asn Asn Asn Asn Asn Asn Phe Ile Arg Asn Asn Asn Asn Asn Asn Asn 115 120 125 Asn Gly Asn Ile Asn Thr Phe His Ala Lys Tyr Pro Cys Ser Phe Asn 130 135 140 Asn Asn Pro Gly Val Ser Cys Ser Ser Ser Pro Ala Gly Val Ile Ser 145 150 155 160 Gly Gly Gly Gln Leu Met Gly Ser Phe Gly Tyr Gly Ala Val Thr Met 165 170 175 Glu Thr Lys Phe Arg Val Arg Gly Gly Ile Pro Ile Pro Asn Gly Ala 180 185 190 Asn Gln Trp Ser Asn Tyr Phe Asn Pro Asn Asn Pro Thr Ser Tyr Ser 195 200 205 Asn Phe Phe Ile Asp Lys Val Ser Ser Asn Val Asp His His Tyr Pro 210 215 220 Tyr Ile Asn His His Asp His Asp His Asp His Asp His His Asp His 225 230 235 240 Asp His Tyr Gly Pro Gln Ser Pro His Glu Ile Gln Thr Leu Pro Leu 245 250 255 Phe Pro Met His Asn Asp His His Asp Leu Asp Leu Asp Leu Asp Leu 260 265 270 Asp His Asp Gln Gly Phe Tyr Thr Tyr Lys Pro Gln Ser Asp Gly Cys 275 280 285 Asn Tyr Thr Gly Gly Tyr Arg Trp Ser Ser Asn Val Asn Gly Gly His 290 295 300 Thr Ser Leu Glu Leu Ser Leu Asn Ser Tyr Gly Phe Gly Cys Tyr Ser 305 310 315 320 Pro Asn <210> 145 <211> 924 <212> DNA <213> Cucumis sativus <400> 145 atggaacatc ctgccaaaca acaacaacaa gaacaaggaa ttaatgatca aggtggtgaa 60 ggtggtggtg gaagcaatgg aaaaggtggg tttttgtgta ggcaaagtag cagtcgatgg 120 actccaacaa ctgaccaaat acgaatattg aaagagcttt attataacaa tggtgttcgc 180 tccccaagcg ctgatcaaat tcagaggatc tctgctaggc ttagacagta cggtaaaatc 240 gaaggcaaaa acgtttttta ttggtttcaa aaccataagg ctcgtgaaag acagaagaaa 300 agattcactt caaattctac ttctcctatt actacctcca atactactac aactactact 360 gattttaaaa tttcttcaac taataataat attaactgga agtctgaaga tcattcctcc 420 tcttctcata acaaatttcc tccaggaata acaattgctt taattgattc ttgtttttgc 480 agctccttct tcttcggtta tggtcgccgt tggccatatg ggaaattacg gatatgggag 540 tgttcaatat cgtcgggagg aaatagcagt gtcgtcggtt accggagcca caacatggga 600 tcatggatcg gtatcgatcc gtattcttct ccggcagctg ccgcggcggg gggatcagcc 660 aatgtgtttg aaaaaacaaa atatgttgaa gaaagcatgg aagatcacga ggagcaagag 720 atcgaaactc tccccctttt ccccattcat ggtgaccgga ataatctcgg tggtttctgc 780 agcatgaagc cggaatattc agagagctac tacaccacca cttggtacgg gcggtctgac 840 gataatggag ccgccggttc tcgggcttcg ctggaactca gcctcaactc ttacgccacc 900 atctcacctg atgatggaat gtga 924 <210> 146 <211> 307 <212> PRT <213> Cucumis sativus <400> 146 Met Glu His Pro Ala Lys Gln Gln Gln Gln Glu Gln Gly Ile Asn Asp 1 5 10 15 Gln Gly Gly Glu Gly Gly Gly Gly Ser Asn Gly Lys Gly Gly Phe Leu 20 25 30 Cys Arg Gln Ser Ser Ser Arg Trp Thr Pro Thr Thr Asp Gln Ile Arg 35 40 45 Ile Leu Lys Glu Leu Tyr Tyr Asn Asn Gly Val Arg Ser Pro Ser Ala 50 55 60 Asp Gln Ile Gln Arg Ile Ser Ala Arg Leu Arg Gln Tyr Gly Lys Ile 65 70 75 80 Glu Gly Lys Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg Glu 85 90 95 Arg Gln Lys Lys Arg Phe Thr Ser Asn Ser Thr Ser Pro Ile Thr Thr 100 105 110 Ser Asn Thr Thr Thr Thr Thr Thr Asp Phe Lys Ile Ser Ser Thr Asn 115 120 125 Asn Asn Ile Asn Trp Lys Ser Glu Asp His Ser Ser Ser Ser His Asn 130 135 140 Lys Phe Pro Pro Gly Ile Thr Ile Ala Leu Ile Asp Ser Cys Phe Cys 145 150 155 160 Ser Ser Phe Phe Phe Gly Tyr Gly Arg Arg Trp Pro Tyr Gly Lys Leu 165 170 175 Arg Ile Trp Glu Cys Ser Ile Ser Ser Gly Gly Asn Ser Ser Val Val 180 185 190 Gly Tyr Arg Ser His Asn Met Gly Ser Trp Ile Gly Ile Asp Pro Tyr 195 200 205 Ser Ser Pro Ala Ala Ala Ala Ala Gly Gly Ser Ala Asn Val Phe Glu 210 215 220 Lys Thr Lys Tyr Val Glu Glu Ser Met Glu Asp His Glu Glu Gln Glu 225 230 235 240 Ile Glu Thr Leu Pro Leu Phe Pro Ile His Gly Asp Arg Asn Asn Leu 245 250 255 Gly Gly Phe Cys Ser Met Lys Pro Glu Tyr Ser Glu Ser Tyr Tyr Thr 260 265 270 Thr Thr Trp Tyr Gly Arg Ser Asp Asp Asn Gly Ala Ala Gly Ser Arg 275 280 285 Ala Ser Leu Glu Leu Ser Leu Asn Ser Tyr Ala Thr Ile Ser Pro Asp 290 295 300 Asp Gly Met 305 <210> 147 <211> 840 <212> DNA <213> Pinus taeda <400> 147 atggaatctg ctgaaagaat cggttcttac gatgtttaca gacaaccatc ttctaccaga 60 tggaacccaa cctctgaaca actttctatc cttagagaac tttactacac caacggtatc 120 agatctccaa ccgttgatga aatccataga atctctatga agctttctag atacggtaag 180 atcgaaggta agaacgtttt ttactggttt caaaaccata aggctagaca tagacaaaag 240 accagacttt ctgctatgaa cgttggtaag ccagctgctt ctccagctct ttctcaaacc 300 accagaaagc ataagcaatg ctacatcacc accaacatcg ttcttaccct taaccaacat 360 tgctacacca agaccaacat ggttcttcaa atcatctggt ctcttggtct tggtaacaag 420 tctcaaacca ccaagatggt ttgggaaatc aagatcaccg cttttccaac catccatacc 480 gaatcttttt acgaaaacag aatggctggt gaaaactctt cttgcaagga agctgcttgc 540 tgctctcttg aacaaatgta cgctgttaag catcatgatg ttatgaaggc tatggaagaa 600 aaccaacttc aagaaggtaa gatcaacaga catgttgaaa cccttgaact ttttccaacc 660 cattgcgaag atagatctcc agaaccatct gaaaagaagt cttgctgctg gggttcttgc 720 gaaaccaagg aaaaccataa caacaaccca ccaggtggtt acaagggtaa gcaaagagat 780 tctgttcttg atctttgcct ttctcttggt aacaagtctt gcggtcttca tgataactaa 840 <210> 148 <211> 279 <212> PRT <213> Pinus taeda <400> 148 Met Glu Ser Ala Glu Arg Ile Gly Ser Tyr Asp Val Tyr Arg Gln Pro 1 5 10 15 Ser Ser Thr Arg Trp Asn Pro Thr Ser Glu Gln Leu Ser Ile Leu Arg 20 25 30 Glu Leu Tyr Tyr Thr Asn Gly Ile Arg Ser Pro Thr Val Asp Glu Ile 35 40 45 His Arg Ile Ser Met Lys Leu Ser Arg Tyr Gly Lys Ile Glu Gly Lys 50 55 60 Asn Val Phe Tyr Trp Phe Gln Asn His Lys Ala Arg His Arg Gln Lys 65 70 75 80 Thr Arg Leu Ser Ala Met Asn Val Gly Lys Pro Ala Ala Ser Pro Ala 85 90 95 Leu Ser Gln Thr Thr Arg Lys His Lys Gln Cys Tyr Ile Thr Thr Asn 100 105 110 Ile Val Leu Thr Leu Asn Gln His Cys Tyr Thr Lys Thr Asn Met Val 115 120 125 Leu Gln Ile Ile Trp Ser Leu Gly Leu Gly Asn Lys Ser Gln Thr Thr 130 135 140 Lys Met Val Trp Glu Ile Lys Ile Thr Ala Phe Pro Thr Ile His Thr 145 150 155 160 Glu Ser Phe Tyr Glu Asn Arg Met Ala Gly Glu Asn Ser Ser Cys Lys 165 170 175 Glu Ala Ala Cys Cys Ser Leu Glu Gln Met Tyr Ala Val Lys His His 180 185 190 Asp Val Met Lys Ala Met Glu Glu Asn Gln Leu Gln Glu Gly Lys Ile 195 200 205 Asn Arg His Val Glu Thr Leu Glu Leu Phe Pro Thr His Cys Glu Asp 210 215 220 Arg Ser Pro Glu Pro Ser Glu Lys Lys Ser Cys Cys Trp Gly Ser Cys 225 230 235 240 Glu Thr Lys Glu Asn His Asn Asn Asn Pro Pro Gly Gly Tyr Lys Gly 245 250 255 Lys Gln Arg Asp Ser Val Leu Asp Leu Cys Leu Ser Leu Gly Asn Lys 260 265 270 Ser Cys Gly Leu His Asp Asn 275 <210> 149 <211> 1500 <212> DNA <213> Arabidopsis thaliana <400> 149 tggcaacttc aatgaattct gaatttaaaa catttttttt tatatatata gatgatttgg 60 cgattttcga actggaatgg tgaaaaagtg gttgagtaca ctattagttt atttatttaa 120 aaaatagagg gaaattcaaa tgaaaaaaaa agtttcatct acagaaaaaa aaacacgtca 180 aaattccaaa aaacttgaac gtcttgaaca acggtataac cgtataagaa agggaactta 240 aaacttaaac gtctacatga ttgaaagggt aaaaatattc atattacaat ggtataagaa 300 aatggagaag ccagaggagc aactccaaag aagaagatgc agcaagagag tgtacattat 360 tataattata agttggtata tatgtagttg ctaaaaaaaa aaaagagtgt acaatatttt 420 ctgaagaagc atcgagctgg ttaatattta agacccacca aaacctacca acaatgttat 480 tctcatctct aagcaaaagt ttgatctcat cttttttttt aatttttttt ttgatgggtc 540 ctcttctacc tctgcatctc cacgtgctat acaagtggcc tcgctgtatg tattatttac 600 cactctctta ttgttactaa aaacttgaat gtcacttgaa tgtcacttga atgcaaacta 660 tatatttagg tgaataccag atcaaatagc aataaataaa ccaacaattt atgctaatta 720 aaaacttgaa aaaacacttt ccattaccta tgatttttta atccaatctt ggggtttgat 780 gcccataaaa cgaagtcgaa aagagaaaag cgaccttaat tgtaataaat gcagctattg 840 acttctctat caacttcaag tcttcaagta actacaaatt taataatctc cttagcttat 900 cttagagaag cacaaaacaa aagggtctac gcttagtctt cctagattct tgatttgagt 960 cttgatggcc acttgctttt tatgtctcaa tcctaagagt tgatacttac ttagtattaa 1020 tcaggtttca cattatctca gacctagctt gcatttttga tacaaacatg tcatgcaata 1080 cacataagca acacgtaccg agtcattgat tgaaaaacat gacctaaata gtaatacgga 1140 ctgagagaga gtgatgaatc atttcatatg ggagacagct ttctcatacg cgtccatgcc 1200 tttgcatgaa agattaaata attcaactct ttcacttatc ttattttaat ttactttgca 1260 gaattccaag ttttattgca atcaccaggg gtattaaatg ggatagtgta aatgcacctg 1320 caacacatca actaaccaca cactcctcca cctgtttgta tttacttcaa aaacttaatt 1380 caaaaatccc tttgcttctt tccttccctc tatttataaa ccctagtatc cggtttttat 1440 tctaccacac aataccattt gcaacaaact tctctctgaa tctcagtgaa ttctcataaa 1500 <210> 150 <211> 1500 <212> DNA <213> Arabidopsis thaliana <400> 150 tattaaacta attaaactta gcctttttca ttagttttgt tactggaaac ggttcgtctt 60 tagtgcacaa agaccaaggt caatttaatt ctttaataaa ttcaattgag agatcagaag 120 cttgtgaatc tggttctctc tctctcttct cttactagct tttgagtcga caaaagagag 180 agaaaagatc attaaactcg aatccaaaac ttcaaccaac aaaagggaaa aaaagaaaaa 240 ataactacaa agagatctat ctttagggtt tcaaaacttt cttgagtttc agatcgaatt 300 gagtaagtat ttatattatt taagtgttct tcttactgta tcatttctga ccatacacca 360 aactagggtt tcttaaagat cataactctc tctctctcgc ttcttcctac actagatctg 420 tccgatagat ctgatgcttt gagaagtctt gtgactttca agattgattt ttctttttgt 480 ttgtttgttt atatgtacac atacttggag atagatatac tctcatatat gttactgtgc 540 atatggatgg atccatctta agtgtgaata aaaactaccc aaatctcatt tttgtttgtt 600 tcttatctca tttgttgttt ttgtaaggcc gtcattataa atctaaaaca atttatccgt 660 tttcgtaaag aaaatcgctt tccaaaaaaa aaaactttga agtaaaaata tcattttggg 720 tctatattca aatctctcct catatttcca tctcattatt tgtaaaatta atatattagg 780 tcattatcat attgattata gagtttatat gttatgttat aaagtaaaag ttatatatgt 840 aaatattacg tttgtaatat atatatttat acatatgtac gtatacataa tttgtatgta 900 tatgtgcctc atttttaaca aatattatcg ttaatcttta gtttcattat aattttttag 960 ggatgtatac attatataaa tattttatgt tatttatatc tgaaatttct atactataac 1020 atcttagata gttttcatac gtaattaaga gttttaatta atagttacat aattatgatt 1080 agatgaattt atctataaat tgtatgcaaa aatatataca tatccaaccg aaaaaatgga 1140 gagtaaacaa aaacctaaca attaatggtt tagtacttgt gaaaatgata aaattattaa 1200 tttaatatat agtttcataa cagctagaaa taactttccc ttaatataat tattttcttc 1260 catttccata atgattttct tcttcttcat tctgtataca tatatgagcg tttcatatat 1320 aatcttttac taattcgatt gtgattttga ttttgggcag tggaagatat acttggagaa 1380 gaaaatcaaa tcacgaaaat atattgatca gtgccttgaa ggaaaccaaa gttgacaact 1440 ccaaaaatcc atttcgttat taatatctaa ctgagtgatc atagtcaatc atccataaca 1500 <210> 151 <211> 1500 <212> DNA <213> Arabidopsis thaliana <400> 151 tccattaatt catgtacaca aatttatttg tttaaagtat cattggatcc attacacttc 60 agttttctta gcatctttat aaaactctca ctttcatgat gttttttttt tataatttaa 120 tatatcctgt atgttgaatt ttcagttagt catctatatt ttgtcttaaa ttttgccttc 180 tttagcatta attaattgat ttcttgcctc taatatcttt cattcctctc ctaatttgct 240 ttctgacttt ctgatttatt gtattcttct ttaaataatc ttatcaattt ctgcatagtc 300 agagatgaaa ccttcaagaa aacaataaag aggaaagatt aaaggttagt attcccttgc 360 ctaccaaaaa aaacagaaag gtttgtattc tcttcgtata tccttatttt cattatacgt 420 ttggttttat atataaacac gtgtatatac tttatagtag tagtatacat ttacatatta 480 atgtgtgtaa taaaaacgtt taaatacgat gatgatgata agaaatagct ggaaaaaatt 540 aatacatcac ccaggcctta tgtcacgttg gttgtaagtt gtataataac tatatgttgg 600 tccgtcgcac aggtgcacag atataatact atttttttca tttcaatttt tttttctttc 660 ttaatcgcca aatagtagtt tacacttttt cctgaagagt ttttattttt tgaaaatcta 720 ctaatgatta tatttaatta aattttgttt tgttctcacg aaaatattgt ttttttaata 780 aacaggatag agttgggaat ttatttgtac ctactttttc ccattttatt tgctttgaac 840 aaatgagtcc aacatttctc ttttcgtctt ctctttccta tcccatctaa atcaaaactg 900 atcaaaatac ctcgaaattt taaattaaat caccatccat caaagctatt atagtgtaga 960 aattttaaag ttttttcttt ggacaacaat tgatttccat ctacactaca tatgatatga 1020 ctcttgaatc aatcatagtt tttatttcag tctgctgatt agtttttttc ttttctttta 1080 aaagggtaaa aaatcggtag tttcgaataa ttttgagtat gtgaaaattc attatttttg 1140 ggttggttat tcaataacaa attaatttgt gattgtgatg tcttttttat gcaaggctct 1200 tttttcttgc tttcaattag tgggtccttt gtttcctaca atcatctcca attcttctgt 1260 ttgcttatat cattttgtat gatcttcaag aagaaagata aacgactccg aagaaagatt 1320 taagttattg ttgttcatac aaaaagaagg ttgtaaagtt gttgacgtgg acaaaagtat 1380 tgccatattt ttccagcctc gaagaaattt cccttcttct tcaaacccta aattttgaca 1440 aaactctcta cttttttttg cttctagggt ttgctttcag tttccggagt ataagaaaag 1500 <210> 152 <211> 1905 <212> DNA <213> Arabidopsis thaliana <400> 152 tttgagccaa cagtcggaaa acaaccatca cttagcattt ttttattttt aatactacta 60 tttttttttt tgtaattagt cgagtgtacc aatcccaaat ttaatgggta tctttcataa 120 ccaaaaagaa aaaagcacaa aaacaaaata ctactactat actataatgg tgaaacacac 180 attacattag cattaaccgc tattccttaa aagatatatg agcagactac aaggaagtac 240 atttaaatca gtgtgctaag ttttctcaat tatcaatcta ataattgtac ttacataaat 300 ttaccaaatc atctcaccat ttagggcata caatttttgc tcaataatgg agcttgaaaa 360 gactaattat agaatgtctt tttggttgac acaagctcgc ttcttcatgt cgtttacttt 420 aaaaattgta gattctttca caacttataa gattctatag cctatttgat tatcctttag 480 gatgatgagt ataatatctc aaactataaa ataaaaatgt tcacatcaat aattgaaaaa 540 aaatgcatgt atgaattcac gaaaatatgg ttacggttgt gtgttgctgc tagacaaaag 600 tctttcatat attcagttat atttcttaaa actaatcaaa attgttttat gcaattttgg 660 cgagtagaga gaaaaagcgc gtgggataaa taaagaaagg cgttataaaa aaagagaaaa 720 agtcaaccga gtagttctgg taagagagtc tcgtgcattc gtaaggtttc caagagtcca 780 catatctatt tcatcatcct cttaacgttt ttctcactta cttttttccc cactcttaat 840 tgattccgtt gataaaattt gttcgttata tagaaataat cagaatataa ttagggttaa 900 ttttggagta aagaccattc aatcaatggt ttggcttgat tcttgtttga aattctatgt 960 ttaaaaaggg taatacttac aaacttgact ttattagtat tttaaaaaat gacaaaactt 1020 aaaatggatt tgaaatctag catatttaac tttaatacag ttttggttta agtttcactc 1080 tcttttctat caaaaatata attttaaaat ttgaaagatg ttaaaataga caaatgtcat 1140 tagtttgttc agatttagtt atgtttttaa tatacagaca attatattaa aataataata 1200 aaaaagtcag atcagttata aaagaaagtc agttcagttt taagacttaa gtttgagtct 1260 tttggatttg aatttaatag attcactttt attaaaaatt gttgaaacac tttttaaaaa 1320 ctaaaacatt tgtcaaaaaa tgcatttaac caaaaaaatg aaaatgaaag gtagtaagta 1380 ataaaagaag aaaacaaaat ctaaaaaagt gtctttaaaa tctcaaaatt aaaatataca 1440 aaaaagtaca agaaaataaa agaaaagggg gaaaaagaga gtcaatatca acatgcaagt 1500 gtgaatatta gactcttctt ctctcatcag ttaccccaaa actagggttt acccattttc 1560 atcatcttct tcttccccac atcactctct gaaagacaat aagagagaga gacacacaca 1620 ccaaaaaaaa aaccccattg atttttaaga gagagagaga gactgagaac gaagagcaaa 1680 tgagagagaa gttgaatcaa atttccgctt tcgttctctt tctctctcct ccatttcagt 1740 aataaacact ccttcctctt taatttttct gggtttcttc ttcttccctc ttttgttgtt 1800 tctgtgtgat tcagttctcg atccatcttt tcgtgaaaag tatcaatctt ttaattctct 1860 agttgtttgt caaatctggt tagtttcttc tcttgtattt atttg 1905 <210> 153 <211> 11210 <212> DNA <213> Artificial Sequence <220> <223> RV021090 T-DNA <400> 153 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggag ctcaaacgag caggaagcaa cgagagggtg 120 gcgcgcgacc gacgtgcgta cgtagcatga gcctgagtgg agacgttgga cgtgtatgta 180 tatacctctc tgcgtgttaa ctatgtacgt aagcggcagg cagtgcaata agtgtggctc 240 tgtagtatgt acgtgcgggt acgatgctgt aagctactga ggcaagtcca taaataaata 300 atgacacgtg cgtgttctat aatctcttcg cttcttcatt tgtccccttg cggagtttgg 360 catccattga tgccgttacg ctgagaacag acacagcaga cgaaccaaaa gtgagttctt 420 gtatgaaact atgacccttc atcgctaggc tcaaacagca ccccgtacga acacagcaaa 480 ttagtcatct aactattagc ccctacatgt ttcagacgat acataaatat agcccatcct 540 tagcaattag ctattggccc tgcccatccc aagcaatgat ctcgaagtat ttttaatata 600 tagtattttt aatatgtagc ttttaaaatt agaagataat tttgagacaa aaatctccaa 660 gtattttttt gggtattttt tactgcctcc gtttttcttt atttctcgtc acctagttta 720 attttgtgct aatcggctat aaacgaaaca gagagaaaag ttactctaaa agcaactcca 780 acagattaga tataaatctt atatcctgcc tagagctgtt aaaaagatag acaactttag 840 tggattagtg tatgcaacaa actctccaaa tttaagtatc ccaactaccc aacgcatatc 900 gttccctttt cattggcgca cgaactttca cctgctatag ccgacgtaca tgttcgtttt 960 ttttgggcgg cgcttacttt cttccccgtt cgttctcagc atcgcaactc aatttgttat 1020 ggcggagaag cccttgtatc ccaggtagta atgcacagat atgcattatt attattcata 1080 aaacgaacgc tcttcaactg gaagagcggt tactaccggt tcactagcta gctgctaatc 1140 gagctagtta ccctatgagg tgacatgaag cgctcacggt tactatgacg gttagcttca 1200 cgactgttgg tggcagtagc gtacgactta gctatagttc cggacttacc tagctaggtt 1260 accagagctg gtcaccttct tcatcgctaa gaagacactc agtagtccgg accgatatcg 1320 ctatcaactt tgtatagaaa agttgggcca gaatggcccg gaccgggtta ccaagcggcc 1380 gcgaattcca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatcgggaaa 1440 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga 1500 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc 1560 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga 1620 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga 1680 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca 1740 tttggagagg acccatggaa actcaacaac aacaagcaga tattcaagat tttgggaaca 1800 aaaacagtaa cacctatgca tgcaggcaaa gcagtactag gtggacaccc acaagtgatc 1860 agatcagaat actgaaagag ctctactaca acaatggaat cagatcacca actgctgatc 1920 agattcagag aatcgctgct caactcagac agtacggcaa gattgaaggc aagaacgtgt 1980 tttattggtt tcagaaccat aaagctcgtg aacgccagaa gaaacggttc actcctactg 2040 ctcctccacc accgtctcac ttctccgatc atattaacca ccatcttccc aacgctgccc 2100 ccatgcagat tccgtctcac caccaccact accaccatca agaaccacca catgtctatg 2160 ctcaccctca caaactctac accactcatc acattggggt tggttcttct tcacaaggag 2220 tgatgggtgt agggtgtggc tatggatctg ttgctatgga gaagagtttc aggaagtgtt 2280 caatatcacc accgggagag agtaaggcca caggaggaat tggccggaat atcggttcaa 2340 gatcacggat aagtgtcgat tcgtgttcct tcttcgacac aattaagcca aagacatatg 2400 agatgtttga gaatcatgat caagatgaag aacaaggaga gccttcaact gagatcgaaa 2460 cacttccatt attcccgatt cacgacggca accatcatga tttcttcggc atgaggacgg 2520 caaatctgca attggagcaa ggcactggag gttactacac tggaggaagc tggtatcggt 2580 ctgatgagag ggcgtctctt gagctcagtc tcaactcata tggttattat aactaattcg 2640 aactggccag gtacctgaca tcttatagtc tgcaacctct cgtgtctgaa ttcctatctt 2700 tatcaagtgt tattgcttcc acgactatag gacagctttc gtcgaaaggt tttgctcatg 2760 tgatctcgaa ggattcatct agtctgattt ttcgtgactt gtatcggttt tattggattc 2820 atccaacata tatcaataaa aaatgagttg tgtttccttt cttcctagtt cagttaaaat 2880 tatttccctc ctgcgcttgt gctgtaattg tctgtgtacc tgttgtttgt gactgtgtta 2940 gttcccttgg atatgatttc gtatttgata tgtacatgga gatagcttag cttcattatt 3000 ggagtatgaa gttagtatga catagtcact ctcctggaaa attgacactg caaaccatat 3060 ttttattctg aaccacaaat cctagtcagt ccgctggcat atgccgtccg tttgctgaat 3120 ccagaacgtg ggtttggaga tgtacggctg agatgcctct atgcgaaggg gatttcgtgg 3180 tgaaacgaga tgggagtaga gcaacgcccg tggaagatgc ttcaaacttc cacacttttg 3240 agcaacgatc ggcagtagta aggtagacga tttcaagatc aaagcatatg aagataaaca 3300 acatcaacaa caaaatttgt tggggttcta tagagagaaa cagagctaca tacatacact 3360 gttttgtatc taccatctga gatgatgaaa agatgaaaaa ctaaagaatg ccccggcgcc 3420 aacgccagga cacgccgcgc gcgcgtcacc cgagccatct cttgacccag ccggcgctgt 3480 atatttacac acgttgcagc atcgatcacc acctgttcga tcgcgtcgcc gtcaccaagc 3540 ttggcgcgcc ggtcacccgg tccgggccta gaaggccacc cggggctcgg cgttaggctt 3600 ttagtccgct aggtggctag accgaggtta ggacgctagt tcactagggg cctagtggtg 3660 ttttaaggat gggcaagttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga 3720 gtgcggccgc cagtgtgatg gatatctcga cccgggtgat tgcggttaca tcatgtacgg 3780 aaaaataatt ctaatccttg atttaaattt gaacttgact atttatttat tctttatttc 3840 attttgtaaa tcattttatg tatctcctgg caagcaattt tatccacctt gcaccaacac 3900 cttcgggttc cataatcaaa ccaccttaac ttcacaccat gctgtaactc acaccgccca 3960 gcatctccaa tgtgaaagaa gctaaaattt aataaacaat catacgaagc agtgacaaaa 4020 taccagatgg tattaatgct tcgataaaat taattggaaa gtataaaatg gtagaaaata 4080 ataaattata attaatttaa gtaagataaa aaataattaa aaactaaaat gttaaaattt 4140 taaaaaaatt attttaaata atatttaaaa acattaaaaa tcattttaaa aaatttattt 4200 atagaacaat taaataaata tttcagctaa taaaaaacaa aagcttacct agccttagaa 4260 gacaacttgt ccaacaatta gatgataccc attgccctta cgttttcttt aacatcaatt 4320 attgtttttg tcaacaagct atcttttagt tttattttat tggtaaaaaa tatgtcgcct 4380 tcaagttgca tcatttaaca catctcgtca ttagaaaaat aaaactcttc cctaaacgat 4440 tagtagaaaa aatcattcga taataaataa gaaagaaaaa ttagaaaaaa ataacttcat 4500 tttaaaaaaa tcattaaggc tatatttttt aaatgactaa ttttatatag actgtaacta 4560 aaagtataca atttattatg ctatgtatct taaagaatta cttataaaaa tctacggaag 4620 aatatcttac aaagtgaaaa acaaatgaga aagaatttag tgggatgatt atgattttat 4680 ttgaaaattg aaaaaataat tattaaagac tttagtggag taagaaagct ttcctattag 4740 tcttttctta tccataaaaa aaaaaaaaaa aatctagcgt gacagctttt ccatagattt 4800 taataatgta aaatactggt agcagccgac cgttcaggta atggacactg tggtcctaac 4860 ttgcaacggg tgcgggccca atttaataac gccgtggtaa cggataaagc caagcgtgaa 4920 gcggtgaagg tacatctctg actccgtcaa gattacgaaa ccgtcaacta cgaaggactc 4980 cccgaaatat catctgtgtc ataaacacca agtcacacca tacatgggca cgcgtcacaa 5040 tatgattgga gaacggttcc accgcatatg ctataaaatg cccccacacc cctcgaccct 5100 aatcgcactt caattgcaat caaattagtt cattctcttt gcgcagttcc ctacctctcc 5160 tttcaaggtt cgtagatttc ttccgttttt ttttcttctt ctttattgtt tgttctacat 5220 cagcatgatg ttgatttgat tgtgttttct atcgtttcat cgattataaa ttttcataat 5280 cagaagattc agcttttatt aatgcaagaa cgtccttaat tgatgatttt ataaccgtaa 5340 attaggtcta attagagttt ttttcataaa gattttcaga tccgtttaca acaagcctta 5400 attgttgatt ctgtagtcgt agattaaggt ttttttcatg aactacttca gatccgttaa 5460 acaacagcct tatttgttga tacttcagtc gtttttcaag aaattgttca gatccgttga 5520 taaaagcctt attcgttgat tctgtatggt atttcaagag atattgctca ggtcctttag 5580 caactacctt atttgttgat tctgtggcca tagattagga ttttttttca cgaaattgct 5640 tcttgaaatt acgtgatgga ttttgattct gatttatctt gtgattgttg actctacagc 5700 catggcccac agcaagcacg gcctgaagga ggagatgacc atgaagtacc acatggaggg 5760 ctgcgtgaac ggccacaagt tcgtgatcac cggcgagggc atcggctacc ccttcaaggg 5820 caagcagacc atcaacctgt gcgtgatcga gggcggcccc ctgcccttca gcgaggacat 5880 cctgagcgcc ggcttcaagt acggcgaccg gatcttcacc gagtaccccc aggacatcgt 5940 ggactacttc aagaacagct gccccgccgg ctacacctgg ggccggagct tcctgttcga 6000 ggacggcgcc gtgtgcatct gtaacgtgga catcaccgtg agcgtgaagg agaactgcat 6060 ctaccacaag agcatcttca acggcgtgaa cttccccgcc gacggccccg tgatgaagaa 6120 gatgaccacc aactgggagg ccagctgcga gaagatcatg cccgtgccta agcagggcat 6180 cctgaagggc gacgtgagca tgtacctgct gctgaaggac ggcggccggt accggtgcca 6240 gttcgacacc gtgtacaagg ccaagagcgt gcccagcaag atgcccgagt ggcacttcat 6300 ccagcacaag ctgctgcggg aggaccggag cgacgccaag aaccagaagt ggcagctgac 6360 cgagcacgcc atcgccttcc ccagcgccct ggcctgagag ctcgaatttc cccgatcgtt 6420 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta 6480 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt 6540 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag 6600 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac 6660 tagatcggga attctagtgg ccggcccagc tgatatctag acccagcttt cttgtacaaa 6720 gtggccgtta acggatctgg ccgcggccgc atcaggatat tcttgtttaa gatgttgaac 6780 tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg 6840 aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata 6900 ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca 6960 ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa 7020 cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac 7080 aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac 7140 gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata 7200 aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga 7260 aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt 7320 atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct 7380 caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa 7440 cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa 7500 tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac 7560 gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga 7620 cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc 7680 ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct 7740 accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc 7800 gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga 7860 cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata 7920 tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt 7980 tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag 8040 tttttctgat taacagccat ggctgcaact actcttacat ctgctttacc tggtgctttc 8100 agttcatccc agagaccaag tgctcctttc aacttacaaa gatcacctag agtgcttaga 8160 aggttcaaca gaaagacagg acgtcaacct cgtggacttg ttcgtgctgc aaaggctcaa 8220 gcggcgcaaa acctgccaga gaaattcgat gaaacaagac tctgggaggc cctcagggga 8280 tttggggtta gtccaagtag ggttacttac gctcccgtcg gattcggaga ttaccactgg 8340 accgtcacag atgaagacgg gagaccttgg ttcgcaactg tcagtgacct cgaacacaaa 8400 gagcattgcg ggcagggagc acaagcagcc cttaaaggac tcagacaagc tatggacact 8460 gccctcgcac ttagagatag ggacggactc agattcgtgg ttgctccagt ggcagcaaca 8520 gatgggggtg gccctgtcct tccccttgac gctaggtatg cactcacagt cttccctcac 8580 gtcccaggaa gaacaggaga gttcggacaa agactcacag aggcagagag ggacagactg 8640 cttgctcttc tggcagaact ccacggaaga acacctcctg aaacaacccc accagccgat 8700 atggaaccac caggacttcc tggtgttaga gcagcactcg ccgaaagtga aggaccttgg 8760 agtggaggac catttgcaga acccgctaga cttcttctca gagaacacga ggccacactg 8820 cacgcaagac tcgctgaatt cgaagccctc gtcgcaagag ttaagggtcg tggagcacct 8880 cttgtcgtta cccacggaga accacaccca ggcaacttga tcctcggaga ggaaggctat 8940 ctcctcgtgg actgggatac agttggactg gctccagctg aaagggattt gagtctcatc 9000 tcagacgacc cagctgacct cgcaagatac gcagagctta caggaagaac tccagaccca 9060 gacgcactcg cactctatag actgcgttgg agtctccttg atgtggctga gttcgtcgag 9120 tggttcaggg ctgaacacca aagaacccaa gacaccgaga gtgcatggaa ggggttcact 9180 gacacacttg ggcaattggc tgctggtgga tcagctgcat agcctaggaa acttatctct 9240 gttatgaatc agaagaagtt catgtctcgt ttcatttaaa actttggtgg tttgtgtttt 9300 ggggccttgt aaagcccctg atgaataatt gttcaactat gtttccgttc ctgtgttata 9360 cctttctttc taatgagtaa tgacatcaaa cttcttctgt attgaaatta tgtccttgtg 9420 agtctcttta tcatcgtttc gtctttacat tatatgtgct acttttgtct aatgagcctg 9480 aaaagtggct ccaatggtac gcactggaag atttgttggc ttctggtaga tatagcgaca 9540 gtgttgagct tgtaatatca tgtctcttat tgctaaatta gttcctttct taacagaaac 9600 cttcaaagtt tttgtttttg ttttcattta cctaatgtac acatacgctg gccatgacta 9660 acaacatgtc caggcttaga gcatattttt ttctagctta aattgttaac ttgtcattca 9720 gtaaaatccg agaattgtga agctctaatt gaagctaatt cgttttataa agtcagttaa 9780 aaagtatact aaattatcca acttttcttc aaaatctcaa aattctatga caaaacgata 9840 gtctttgttt atgtcagtac cacaaagagg tggaaaaaaa caccaaaaaa acaataagca 9900 aactatacac tgagaagaaa aataaaagag agctcaatag atgttttata ctaacggtag 9960 attagatcaa agatccaagc tttactctac atagagcaga acccagaatc ccttcatatc 10020 tcttttattc tagcaccgat aatctactga aaagaagaca cttagagctc tgtctctttg 10080 tcaaagaagt cccagccgtc atccagaagc tccttacgtt cattaacaga ggtaccggcg 10140 gccgcgggca actttattat acaaagttga tagatatcgg tccgacggcc agaatggcca 10200 tctggattca gcaggcctag aaggccattt agtcctagcg atcgcgctga caagcggtat 10260 ttaaatcatc attctaggcg cgccacttac gcttgattcc gatgacttcg taggttccta 10320 gctcaagccg ctcgtgtcca agcgtcactt acgattagct aatgattacg gcatctagga 10380 ccgactagtt cttatgtgct tctagtctcc aaatgtggtt gatagttatt ttgctctaag 10440 atcaacagta atgaagtata aatcatcgtt gtggtgtgct actcggttaa ttgagcatta 10500 acacacacaa acatgacgag gatggtataa tctccaaaaa tgtgtacttt gttaggtggg 10560 accctatagc cttgattaat gtgctatgtt aggcatgcct ggaaacgtgt gacgcatatg 10620 ttttgtgaac ctgttgatat tatatgtgct tttatattac catattttat taaaatacta 10680 atatttatta ctagtaagat ataacattct atctagctta aaaactaacc ataaatattc 10740 cataataact agatttacca aactaatata ctaaatatac ataataaata caaaattaac 10800 aagacaataa tcaatattta tgagcttaat atatttagac attatggttg gtcgacgata 10860 atcatgctaa cttttcgtaa ttgcttgatt gaaatatgct tagaataatg cctctttgtt 10920 ctacatggca aatagggacc attatggtgt aacaccctgg gaaccacaaa caccccgaaa 10980 tgctactaaa ctacacaact aaccttcata tataaaattt cgacagcatc tcctttgaaa 11040 atttgcatag acgtggaagc aacagagtat aaacagatct agctagctaa gtttaaacgg 11100 ccggccaaac gtgcaagcgc tactagacaa ttcagtacat taaaaacgtc cgcaatgtgt 11160 tattaagttg tctaagcgtc aatttattta caattgaata tatcctgcca 11210 <210> 154 <211> 15172 <212> DNA <213> Artificial Sequence <220> <223> RV026522 T-DNA <400> 154 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaagaaaaca gcaacagcag cagaagcagc aacagcggcg cacgttggaa tccgacgccg 1860 gagcagctga gcattctgaa agagctgtat catggccgcg gaatcaggtc gcccagcgcc 1920 gaacagatcc accatatctc atggaaactc agctcctacg gcaaaatcga gggcaagaac 1980 gtcttctact ggttccagaa ccataaggcc cgccagcgtc aaaaagagcg tctcggttcc 2040 gtcatgcctg cttctgcttc ttcttcttct tttgcatctc cttctcaggg agctgtcggc 2100 attcgaaggg gaagtacggt gctatgtctt aaggaaactt cagcttcatc ttcatcttat 2160 tcttctgttc cgcgttcggt atcagctgtg cgccagtgtg ctgcatctcg tgactgtggc 2220 ttgcaccatg atctgacaac tctggagctg tttcccctgc accgtgaaag tagaatagac 2280 gctggggagg aagacgagga gcaacaggag gtggtggtgc tagataataa taataacacc 2340 tggacaaggc accaaaatgt aaatgtgaat catgcaaata atgacgatga gccacagcat 2400 ggtcatgggc agattgatat tgatgtaggg cttcgtcttt gcctcggtgg atttcgctga 2460 ggtacctgac atcttatagt ctgcaacctc tcgtgtctga attcctatct ttatcaagtg 2520 ttattgcttc cacgactata ggacagcttt cgtcgaaagg ttttgctcat gtgatctcga 2580 aggattcatc tagtctgatt tttcgtgact tgtatcggtt ttattggatt catccaacat 2640 atatcaataa aaaatgagtt gtgtttcctt tcttcctagt tcagttaaaa ttatttccct 2700 cctgcgcttg tgctgtaatt gtctgtgtac ctgttgtttg tgactgtgtt agttcccttg 2760 gatatgattt cgtatttgat atgtacatgg agatagctta gcttcattat tggagtatga 2820 agttagtatg acatagtcac tctcctggaa aattgacact gcaaaccata tttttattct 2880 gaaccacaaa tcctagtcag tccgctggca tatgccgtcc gtttgctgaa tccagaacgt 2940 gggtttggag atgtacggct gagatgcctc tatgcgaagg ggatttcgtg gtgaaacgag 3000 atgggagtag agcaacgccc gtggaagatg cttcaaactt ccacactttt gagcaacgat 3060 cggcagtagt aaggtagacg atttcaagat caaagcatat gaagataaac aacatcaaca 3120 acaaaatttg ttggggttct atagagagaa acagagctac atacatacac tgttttgtat 3180 ctaccatctg agatgatgaa aagatgaaaa actaaagaat gccccggcgc caacgccagg 3240 acacgccgcg cgcgcgtcac ccgagccatc tcttgaccca gccggcgctg tatatttaca 3300 cacgttgcag catcgatcac cacctgttcg atcgcgtcgc cgtcaccaag cttggcgcgc 3360 cggtcacccg gtccgggcct agaaggccac ccggggctcg gcgttaggct tttagtccgc 3420 taggtggcta gaccgaggtt aggacgctag ttcactaggg gcctagtggt gttttaagga 3480 tgggcaagtt tgtacaaaaa agcaggctgg cgccggaacc aattcagtcg agtgcggccg 3540 ccagtgtgat ggatatctcg acccgggtga ttgcggttac atcatgtacg gaaaaataat 3600 tctaatcctt gatttaaatt tgaacttgac tatttattta ttctttattt cattttgtaa 3660 atcattttat gtatctcctg gcaagcaatt ttatccacct tgcaccaaca ccttcgggtt 3720 ccataatcaa accaccttaa cttcacacca tgctgtaact cacaccgccc agcatctcca 3780 atgtgaaaga agctaaaatt taataaacaa tcatacgaag cagtgacaaa ataccagatg 3840 gtattaatgc ttcgataaaa ttaattggaa agtataaaat ggtagaaaat aataaattat 3900 aattaattta agtaagataa aaaataatta aaaactaaaa tgttaaaatt ttaaaaaaat 3960 tattttaaat aatatttaaa aacattaaaa atcattttaa aaaatttatt tatagaacaa 4020 ttaaataaat atttcagcta ataaaaaaca aaagcttacc tagccttaga agacaacttg 4080 tccaacaatt agatgatacc cattgccctt acgttttctt taacatcaat tattgttttt 4140 gtcaacaagc tatcttttag ttttatttta ttggtaaaaa atatgtcgcc ttcaagttgc 4200 atcatttaac acatctcgtc attagaaaaa taaaactctt ccctaaacga ttagtagaaa 4260 aaatcattcg ataataaata agaaagaaaa attagaaaaa aataacttca ttttaaaaaa 4320 atcattaagg ctatattttt taaatgacta attttatata gactgtaact aaaagtatac 4380 aatttattat gctatgtatc ttaaagaatt acttataaaa atctacggaa gaatatctta 4440 caaagtgaaa aacaaatgag aaagaattta gtgggatgat tatgatttta tttgaaaatt 4500 gaaaaaataa ttattaaaga ctttagtgga gtaagaaagc tttcctatta gtcttttctt 4560 atccataaaa aaaaaaaaaa aaatctagcg tgacagcttt tccatagatt ttaataatgt 4620 aaaatactgg tagcagccga ccgttcaggt aatggacact gtggtcctaa cttgcaacgg 4680 gtgcgggccc aatttaataa cgccgtggta acggataaag ccaagcgtga agcggtgaag 4740 gtacatctct gactccgtca agattacgaa accgtcaact acgaaggact ccccgaaata 4800 tcatctgtgt cataaacacc aagtcacacc atacatgggc acgcgtcaca atatgattgg 4860 agaacggttc caccgcatat gctataaaat gcccccacac ccctcgaccc taatcgcact 4920 tcaattgcaa tcaaattagt tcattctctt tgcgcagttc cctacctctc ctttcaaggt 4980 tcgtagattt cttccgtttt tttttcttct tctttattgt ttgttctaca tcagcatgat 5040 gttgatttga ttgtgttttc tatcgtttca tcgattataa attttcataa tcagaagatt 5100 cagcttttat taatgcaaga acgtccttaa ttgatgattt tataaccgta aattaggtct 5160 aattagagtt tttttcataa agattttcag atccgtttac aacaagcctt aattgttgat 5220 tctgtagtcg tagattaagg tttttttcat gaactacttc agatccgtta aacaacagcc 5280 ttatttgttg atacttcagt cgtttttcaa gaaattgttc agatccgttg ataaaagcct 5340 tattcgttga ttctgtatgg tatttcaaga gatattgctc aggtccttta gcaactacct 5400 tatttgttga ttctgtggcc atagattagg attttttttc acgaaattgc ttcttgaaat 5460 tacgtgatgg attttgattc tgatttatct tgtgattgtt gactctacag ccatggccca 5520 cagcaagcac ggcctgaagg aggagatgac catgaagtac cacatggagg gctgcgtgaa 5580 cggccacaag ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcagac 5640 catcaacctg tgcgtgatcg agggcggccc cctgcccttc agcgaggaca tcctgagcgc 5700 cggcttcaag tacggcgacc ggatcttcac cgagtacccc caggacatcg tggactactt 5760 caagaacagc tgccccgccg gctacacctg gggccggagc ttcctgttcg aggacggcgc 5820 cgtgtgcatc tgtaacgtgg acatcaccgt gagcgtgaag gagaactgca tctaccacaa 5880 gagcatcttc aacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccac 5940 caactgggag gccagctgcg agaagatcat gcccgtgcct aagcagggca tcctgaaggg 6000 cgacgtgagc atgtacctgc tgctgaagga cggcggccgg taccggtgcc agttcgacac 6060 cgtgtacaag gccaagagcg tgcccagcaa gatgcccgag tggcacttca tccagcacaa 6120 gctgctgcgg gaggaccgga gcgacgccaa gaaccagaag tggcagctga ccgagcacgc 6180 catcgccttc cccagcgccc tggcctgaga gctcgaattt ccccgatcgt tcaaacattt 6240 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat 6300 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga 6360 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa 6420 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg 6480 aattctagtg gccggcccag ctgatatcta gacccagctt tcttgtacaa agtggccgtt 6540 aacggatcgg ccagaatggc ccggaccggg ttaccgaatt cgagctcggt accctgggat 6600 ccgatatcga tgggccctgg ccgaagcttg gtcacccggt ccgggcctag aaggccagct 6660 tcggccgccc cgggcaactt tattatacaa agttgatggt acggatccgt cgacggcgcg 6720 ccgcggacct gaattccagc acactggcgg ccgttagtct aaaaacggcc cctaactaac 6780 gcttagttgc ccgctagatt agtcccccgt tagtgcctag ttagacacac gacgaactag 6840 gggctactag taacggccgc cagtgtgctc ggtacggcca gaatggccat ctggattcag 6900 cagggggtga ttgcggttac atcatgtacg gaaaaataat tctaatcctt gatttaaatt 6960 tgaacttgac tatttattta ttctttattt cattttgtaa atcattttat gtatctcctg 7020 gcaagcaatt ttatccacct tgcaccaaca ccttcgggtt ccataatcaa accaccttaa 7080 cttcacacca tgctgtaact cacaccgccc agcatctcca atgtgaaaga agctaaaatt 7140 taataaacaa tcatacgaag cagtgacaaa ataccagatg gtattaatgc ttcgataaaa 7200 ttaattggaa agtataaaat ggtagaaaat aataaattat aattaattta agtaagataa 7260 aaaataatta aaaactaaaa tgttaaaatt ttaaaaaaat tattttaaat aatatttaaa 7320 aacattaaaa atcattttaa aaaatttatt tatagaacaa ttaaataaat atttcagcta 7380 ataaaaaaca aaagcttacc tagccttaga agacaacttg tccaacaatt agatgatacc 7440 cattgccctt acgttttctt taacatcaat tattgttttt gtcaacaagc tatcttttag 7500 ttttatttta ttggtaaaaa atatgtcgcc ttcaagttgc atcatttaac acatctcgtc 7560 attagaaaaa taaaactctt ccctaaacga ttagtagaaa aaatcattcg ataataaata 7620 agaaagaaaa attagaaaaa aataacttca ttttaaaaaa atcattaagg ctatattttt 7680 taaatgacta attttatata gactgtaact aaaagtatac aatttattat gctatgtatc 7740 ttaaagaatt acttataaaa atctacggaa gaatatctta caaagtgaaa aacaaatgag 7800 aaagaattta gtgggatgat tatgatttta tttgaaaatt gaaaaaataa ttattaaaga 7860 ctttagtgga gtaagaaagc tttcctatta gtcttttctt atccataaaa aaaaaaaaaa 7920 aaatctagcg tgacagcttt tccatagatt ttaataatgt aaaatactgg tagcagccga 7980 ccgttcaggt aatggacact gtggtcctaa cttgcaacgg gtgcgggccc aatttaataa 8040 cgccgtggta acggataaag ccaagcgtga agcggtgaag gtacatctct gactccgtca 8100 agattacgaa accgtcaact acgaaggact ccccgaaata tcatctgtgt cataaacacc 8160 aagtcacacc atacatgggc acgcgtcaca atatgattgg agaacggttc caccgcatat 8220 gctataaaat gcccccacac ccctcgaccc taatcgcact tcaattgcaa tcaaattagt 8280 tcattctctt tgcgcagttc cctacctctc ctttcaaggt tcgtagattt cttccgtttt 8340 tttttcttct tctttattgt ttgttctaca tcagcatgat gttgatttga ttgtgttttc 8400 tatcgtttca tcgattataa attttcataa tcagaagatt cagcttttat taatgcaaga 8460 acgtccttaa ttgatgattt tataaccgta aattaggtct aattagagtt tttttcataa 8520 agattttcag atccgtttac aacaagcctt aattgttgat tctgtagtcg tagattaagg 8580 tttttttcat gaactacttc agatccgtta aacaacagcc ttatttgttg atacttcagt 8640 cgtttttcaa gaaattgttc agatccgttg ataaaagcct tattcgttga ttctgtatgg 8700 tatttcaaga gatattgctc aggtccttta gcaactacct tatttgttga ttctgtggcc 8760 atagattagg attttttttc acgaaattgc ttcttgaaat tacgtgatgg attttgattc 8820 tgatttatct tgtgattgtt gactctacag ccatggctgc aactactctt acatctgctt 8880 tacctggtgc tttcagttca tcccagagac caagtgctcc tttcaactta caaagatcac 8940 ctagagtgct tagaaggttc aacagaaaga caggacgtca acctcgtgga cttgttcgtg 9000 ctgcaaaggc tcaagcggcg caaaacctgc cagagaaatt cgatgaaaca agactctggg 9060 aggccctcag gggatttggg gttagtccaa gtagggttac ttacgctccc gtcggattcg 9120 gagattacca ctggaccgtc acagatgaag acgggagacc ttggttcgca actgtcagtg 9180 acctcgaaca caaagagcat tgcgggcagg gagcacaagc agcccttaaa ggactcagac 9240 aagctatgga cactgccctc gcacttagag atagggacgg actcagattc gtggttgctc 9300 cagtggcagc aacagatggg ggtggccctg tccttcccct tgacgctagg tatgcactca 9360 cagtcttccc tcacgtccca ggaagaacag gagagttcgg acaaagactc acagaggcag 9420 agagggacag actgcttgct cttctggcag aactccacgg aagaacacct cctgaaacaa 9480 ccccaccagc cgatatggaa ccaccaggac ttcctggtgt tagagcagca ctcgccgaaa 9540 gtgaaggacc ttggagtgga ggaccatttg cagaacccgc tagacttctt ctcagagaac 9600 acgaggccac actgcacgca agactcgctg aattcgaagc cctcgtcgca agagttaagg 9660 gtcgtggagc acctcttgtc gttacccacg gagaaccaca cccaggcaac ttgatcctcg 9720 gagaggaagg ctatctcctc gtggactggg atacagttgg actggctcca gctgaaaggg 9780 atttgagtct catctcagac gacccagctg acctcgcaag atacgcagag cttacaggaa 9840 gaactccaga cccagacgca ctcgcactct atagactgcg ttggagtctc cttgatgtgg 9900 ctgagttcgt cgagtggttc agggctgaac accaaagaac ccaagacacc gagagtgcat 9960 ggaaggggtt cactgacaca cttgggcaat tggctgctgg tggatcagct gcatagcaaa 10020 cttatctctg ttatgaatca gaagaagttc atgtctcgtt tcatttaaaa ctttggtggt 10080 ttgtgttttg gggccttgta aagcccctga tgaataattg ttcaactatg tttccgttcc 10140 tgtgttatac ctttctttct aatgagtaat gacatcaaac ttcttctgta ttgaaattat 10200 gtccttgtga gtctctttat catcgtttcg tctttacatt atatgtgcta cttttgtcta 10260 atgagcctga aaagtggctc caatggtacg cactggaaga tttgttggct tctggtagat 10320 atagcgacag tgttgagctt gtaatatcat gtctcttatt gctaaattag ttcctttctt 10380 aacagaaacc ttcaaagttt ttgtttttgt tttcatttac ctaatgtaca catacgctgg 10440 ccatgactaa caacatgtcc aggcttagag catatttttt tctagcttaa attgttaact 10500 tgtcattcag taaaatccga gaattgtgaa gctctaattg aagctaattc gttttataaa 10560 gtcagttaaa aagtatacta aattatccaa cttttcttca aaatctcaaa attctatgac 10620 aaaacgatag tctttgttta tgtcagtacc acaaagaggt ggaaaaaaac accaaaaaaa 10680 caataagcaa actatacact gagaagaaaa ataaaagaga gctcaataga tgttttatac 10740 taacggtaga ttagatcaaa gatccaagct ttactctaca tagagcagaa cccagaatcc 10800 cttcatatct cttttattct agcaccgata atctactgaa aagaagacac ttagagctct 10860 gtctctttgt caaagaagtc ccagccgtca tccagaagct ccttacgttc attaacagag 10920 gccgcaattg gatcgggttt acttattttg tgggtatcta tacttttatt agatttttaa 10980 tcaggctcct gatttctttt tatttcgatt gaattcctga acttgtatta ttcagtagat 11040 cgaataaatt ataaaaagat aaaatcataa aataatattt tatcctatca atcatattaa 11100 agcaatgaat atgtaaaatt aatcttatct ttattttaaa aaatcatata ggtttagtat 11160 ttttttaaaa ataaagatag gattagtttt actattcact gcttattact tttaaaaaaa 11220 tcataaaggt ttagtatttt tttaaaataa atataggaat agttttacta ttcactgctt 11280 taatagaaaa atagtttaaa atttaagata gttttaatcc cagcatttgc cacgtttgaa 11340 cgtgagccga aacgatgtcg ttacattatc ttaacctagc tgaaacgatg tcgtcataat 11400 atcgccaaat gccaactgga ctacgtcgaa cccacaaatc ccacaaagcg cgtgaaatca 11460 aatcgctcaa accacaaaaa agaacaacgc gtttgttaca cgctcaatcc cacgcgagta 11520 gagcacagta accttcaaat aagcgaatgg ggcataatca gaaatccgaa ataaacctag 11580 gggcattatc ggaaatgaaa agtagctcac tcaatataaa aatctaggaa ccctagtttt 11640 cgttatcact ctgtgctccc tcgctctatt tctcagtctc tgtgtttgcg gctgaggatt 11700 ccgaacgagt gaccttcttc gtttctcgca aaggtaacag cctctgctct tgtctcttcg 11760 attcgatcta tgcctgtctc ttatttacga tgatgtttct tcggttatgt ttttttattt 11820 atgctttatg ctgttgatgt tcggttgttt gtttcgcttt gtttttgtgg ttcagttttt 11880 taggattctt ttggtttttg aatcgattaa tcggaagaga ttttcgagtt atttggtgtg 11940 ttggaggtga atcttttttt tgaggtcata gatctgttgt atttgtgtta taaacatgcg 12000 actttgtatg attttttacg aggttatgat gttctggttg ttttattatg aatctgttga 12060 gacagaacca tgatttttgt tgatgttcgt ttacactatt aaaggtttgt tttaacagga 12120 ttaaaagttt tttaagcatg ttgaaggagt cttgtagata tgtaaccgtc gatagttttt 12180 ttgtgggttt gttcacatgt tatcaagctt aatcttttac tatgtatgcg accatatctg 12240 gatccagcaa aggcgatttt ttaattcctt gtgaaacttt tgtaatatga agttgaaatt 12300 ttgttattgg taaactataa atgtgtgaag ttggagtata cctttacctt cttatttggc 12360 tttgtgatag tttaatttat atgtattttg agttctgact tgtatttctt tgaattgatt 12420 ctagtttaag taatcatggc ctcctccgag aacgtcatca ccgagttcat gcgcttcaag 12480 gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 12540 cgcccctacg agggccacaa caccgtgaag ctgaaggtga cgaagggcgg ccccctgccc 12600 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac 12660 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc 12720 gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc aggactcctc cctgcaggac 12780 ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga cggccccgtg 12840 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 12900 gtgctgaagg gcgagaccca caaggccctg aagctgaagg acggcggcca ctacctggtg 12960 gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg 13020 gacgccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag 13080 cgcaccgagg gccgccacca cctgttcctg tagttttgtg atctgatgat aagtggttgg 13140 ttcgtgtctc atgcacttgg gaggtgatct atttcacctg gtgtagtttg tgtttccgtc 13200 agttggaaaa acttatccct atcgatttcg ttttcatttt ctgcttttct tttatgtacc 13260 ttcgtttggg cttgtaacgg gcctttgtat ttcaactctc aataataatc caagtgcatg 13320 ttaaacaatt tgtcatctgt ttcggctttg atatactact ggtgaagatg ggccgtacta 13380 ctgcatcaca acgaaaaata ataataagat gaaaaacttg aagtggaaaa aaaaaaaaac 13440 ttgaatgttc actactactc attgaccata atgtttaaca tacatagctc aatagtattt 13500 ttgtgaatat ggcaacacaa acagtccaaa acaattgtct cttactatac caaaccaagg 13560 gcgccgcttg tttgccactc tttgtgtgca atagtgtgat taccacatct ccacattcaa 13620 tatattccct gaattatctg acgattttga tggctcactg ttttcccaag tcttgaattg 13680 tcttctgtgc gccagtcaaa tgcatatgtg ttgagtttat cttttaaata tcaagctttt 13740 gtttttaact tttgtttgta accaaaaact cacagtagga gtttgatcac ataattttat 13800 gtttgccttt gcaatttcta gtgagtcttt gattaaaagc ttgaaaagaa aatgcagcca 13860 agcttaccaa gtaagttatg tgtattaacc agaggaagag agaatcttgc aaaatttcaa 13920 caaacacaaa aagaagtatt actacgattg gtggagaaag aaaacgattc caaatcttga 13980 actgttgttg taaaagcata gcagaaagtg ggagacaacc gaaatagaaa tgactataac 14040 ttaatttaat gttatcatta taatttcttc tagcaaatat ttagaaagta aatatcacat 14100 caacctttaa tgtaattaag ctttctcttt ttgattcatg tgagatgaaa agaaaaaaaa 14160 gaagagaaaa gtgtagaaaa cacatcattt ctaagctgaa gctgaggtac aaattaatta 14220 ataagtgact agggtcacgt gaccctagtc acttatgatt ccgatgactt cgtaggttcc 14280 tagctcaagc cgctcgtgtc caagcgtcac ttacgattag ctaatgatta cggcatctag 14340 gaccgactag ttcttatgtg cttctagtct ccaaatgtgg ttgatagtta ttttgctcta 14400 agatcaacag taatgaagta taaatcatcg ttgtggtgtg ctactcggtt aattgagcat 14460 taacacacac aaacatgacg aggatggtat aatctccaaa aatgtgtact ttgttaggtg 14520 ggaccctata gccttgatta atgtgctatg ttaggcatgc ctggaaacgt gtgacgcata 14580 tgttttgtga acctgttgat attatatgtg cttttatatt accatatttt attaaaatac 14640 taatatttat tactagtaag atataacatt ctatctagct taaaaactaa ccataaatat 14700 tccataataa ctagatttac caaactaata tactaaatat acataataaa tacaaaatta 14760 acaagacaat aatcaatatt tatgagctta atatatttag acattatggt tggtcgacga 14820 taatcatgct aacttttcgt aattgcttga ttgaaatatg cttagaataa tgcctctttg 14880 ttctacatgg caaataggga ccattatggt gtaacaccct gggaaccaca aacaccccga 14940 aatgctacta aactacacaa ctaaccttca tatataaaat ttcgacagca tctcctttga 15000 aaatttgcat agacgtggaa gcaacagagt ataaacagat ctagctagct aagtttaaac 15060 ggccggccaa acgtgcaagc gctactagac aattcagtac attaaaaacg tccgcaatgt 15120 gttattaagt tgtctaagcg tcaatttatt tacaattgaa tatatcctgc ca 15172 <210> 155 <211> 15352 <212> DNA <213> Artificial Sequence <220> <223> RV026526 T-DNA <400> 155 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaacctcaac aacagctcca gcagcagcag caacaaaacc aacaacagcc aaacgaggat 1860 agcggcagca gcaaaggcag ttttctgtgc aggcaaagca gtacacgctg gactcctaca 1920 actgaccaga taagaatatt gaaggacctt tactacaaca atggagttag gtccccaagt 1980 gctgaacaga ttcagaggat ctcagctagg ctgaggcagt acggcaagat cgaaggcaag 2040 aacgtctttt attggtttca gaaccataaa gctcgtgaaa ggcagaagaa gcgattcact 2100 actgatatgc ccatgcaaag atcccttgga aatgccggtt ggagacctga tgatcccatt 2160 cacaacaaat ttcataccat tcccactcct gggatatctt ccccatcttc ttcttcttca 2220 cccagtgtgc ttgctgttgg gcagatggga agctttggat atggatctgt ggagaggagt 2280 tttacagact gttcaatatc agcaggtggt ggtcgtggtg gggttggtgg atctataaac 2340 cagagcttcg aatgggttgg catggaccca tattcttcat cttatgcact ctttgacaag 2400 agaaaaacaa tgggtgaaag cttcgaagaa gagcaagaag aagaagcaac tccggagatt 2460 gaaacgctcc cactctttcc catgcacgct gaggatatca ctggcttttg caacattaag 2520 cccgaatccg acgcctacta ctccggctgg tacaggcccg ccgacgccaa gaccagttcc 2580 cgcacttctc ttgagcttag cctcaactcc tacgccggca ggtccccgga ttccccctga 2640 ggtacctgac atcttatagt ctgcaacctc tcgtgtctga attcctatct ttatcaagtg 2700 ttattgcttc cacgactata ggacagcttt cgtcgaaagg ttttgctcat gtgatctcga 2760 aggattcatc tagtctgatt tttcgtgact tgtatcggtt ttattggatt catccaacat 2820 atatcaataa aaaatgagtt gtgtttcctt tcttcctagt tcagttaaaa ttatttccct 2880 cctgcgcttg tgctgtaatt gtctgtgtac ctgttgtttg tgactgtgtt agttcccttg 2940 gatatgattt cgtatttgat atgtacatgg agatagctta gcttcattat tggagtatga 3000 agttagtatg acatagtcac tctcctggaa aattgacact gcaaaccata tttttattct 3060 gaaccacaaa tcctagtcag tccgctggca tatgccgtcc gtttgctgaa tccagaacgt 3120 gggtttggag atgtacggct gagatgcctc tatgcgaagg ggatttcgtg gtgaaacgag 3180 atgggagtag agcaacgccc gtggaagatg cttcaaactt ccacactttt gagcaacgat 3240 cggcagtagt aaggtagacg atttcaagat caaagcatat gaagataaac aacatcaaca 3300 acaaaatttg ttggggttct atagagagaa acagagctac atacatacac tgttttgtat 3360 ctaccatctg agatgatgaa aagatgaaaa actaaagaat gccccggcgc caacgccagg 3420 acacgccgcg cgcgcgtcac ccgagccatc tcttgaccca gccggcgctg tatatttaca 3480 cacgttgcag catcgatcac cacctgttcg atcgcgtcgc cgtcaccaag cttggcgcgc 3540 cggtcacccg gtccgggcct agaaggccac ccggggctcg gcgttaggct tttagtccgc 3600 taggtggcta gaccgaggtt aggacgctag ttcactaggg gcctagtggt gttttaagga 3660 tgggcaagtt tgtacaaaaa agcaggctgg cgccggaacc aattcagtcg agtgcggccg 3720 ccagtgtgat ggatatctcg acccgggtga ttgcggttac atcatgtacg gaaaaataat 3780 tctaatcctt gatttaaatt tgaacttgac tatttattta ttctttattt cattttgtaa 3840 atcattttat gtatctcctg gcaagcaatt ttatccacct tgcaccaaca ccttcgggtt 3900 ccataatcaa accaccttaa cttcacacca tgctgtaact cacaccgccc agcatctcca 3960 atgtgaaaga agctaaaatt taataaacaa tcatacgaag cagtgacaaa ataccagatg 4020 gtattaatgc ttcgataaaa ttaattggaa agtataaaat ggtagaaaat aataaattat 4080 aattaattta agtaagataa aaaataatta aaaactaaaa tgttaaaatt ttaaaaaaat 4140 tattttaaat aatatttaaa aacattaaaa atcattttaa aaaatttatt tatagaacaa 4200 ttaaataaat atttcagcta ataaaaaaca aaagcttacc tagccttaga agacaacttg 4260 tccaacaatt agatgatacc cattgccctt acgttttctt taacatcaat tattgttttt 4320 gtcaacaagc tatcttttag ttttatttta ttggtaaaaa atatgtcgcc ttcaagttgc 4380 atcatttaac acatctcgtc attagaaaaa taaaactctt ccctaaacga ttagtagaaa 4440 aaatcattcg ataataaata agaaagaaaa attagaaaaa aataacttca ttttaaaaaa 4500 atcattaagg ctatattttt taaatgacta attttatata gactgtaact aaaagtatac 4560 aatttattat gctatgtatc ttaaagaatt acttataaaa atctacggaa gaatatctta 4620 caaagtgaaa aacaaatgag aaagaattta gtgggatgat tatgatttta tttgaaaatt 4680 gaaaaaataa ttattaaaga ctttagtgga gtaagaaagc tttcctatta gtcttttctt 4740 atccataaaa aaaaaaaaaa aaatctagcg tgacagcttt tccatagatt ttaataatgt 4800 aaaatactgg tagcagccga ccgttcaggt aatggacact gtggtcctaa cttgcaacgg 4860 gtgcgggccc aatttaataa cgccgtggta acggataaag ccaagcgtga agcggtgaag 4920 gtacatctct gactccgtca agattacgaa accgtcaact acgaaggact ccccgaaata 4980 tcatctgtgt cataaacacc aagtcacacc atacatgggc acgcgtcaca atatgattgg 5040 agaacggttc caccgcatat gctataaaat gcccccacac ccctcgaccc taatcgcact 5100 tcaattgcaa tcaaattagt tcattctctt tgcgcagttc cctacctctc ctttcaaggt 5160 tcgtagattt cttccgtttt tttttcttct tctttattgt ttgttctaca tcagcatgat 5220 gttgatttga ttgtgttttc tatcgtttca tcgattataa attttcataa tcagaagatt 5280 cagcttttat taatgcaaga acgtccttaa ttgatgattt tataaccgta aattaggtct 5340 aattagagtt tttttcataa agattttcag atccgtttac aacaagcctt aattgttgat 5400 tctgtagtcg tagattaagg tttttttcat gaactacttc agatccgtta aacaacagcc 5460 ttatttgttg atacttcagt cgtttttcaa gaaattgttc agatccgttg ataaaagcct 5520 tattcgttga ttctgtatgg tatttcaaga gatattgctc aggtccttta gcaactacct 5580 tatttgttga ttctgtggcc atagattagg attttttttc acgaaattgc ttcttgaaat 5640 tacgtgatgg attttgattc tgatttatct tgtgattgtt gactctacag ccatggccca 5700 cagcaagcac ggcctgaagg aggagatgac catgaagtac cacatggagg gctgcgtgaa 5760 cggccacaag ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcagac 5820 catcaacctg tgcgtgatcg agggcggccc cctgcccttc agcgaggaca tcctgagcgc 5880 cggcttcaag tacggcgacc ggatcttcac cgagtacccc caggacatcg tggactactt 5940 caagaacagc tgccccgccg gctacacctg gggccggagc ttcctgttcg aggacggcgc 6000 cgtgtgcatc tgtaacgtgg acatcaccgt gagcgtgaag gagaactgca tctaccacaa 6060 gagcatcttc aacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccac 6120 caactgggag gccagctgcg agaagatcat gcccgtgcct aagcagggca tcctgaaggg 6180 cgacgtgagc atgtacctgc tgctgaagga cggcggccgg taccggtgcc agttcgacac 6240 cgtgtacaag gccaagagcg tgcccagcaa gatgcccgag tggcacttca tccagcacaa 6300 gctgctgcgg gaggaccgga gcgacgccaa gaaccagaag tggcagctga ccgagcacgc 6360 catcgccttc cccagcgccc tggcctgaga gctcgaattt ccccgatcgt tcaaacattt 6420 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat 6480 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga 6540 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa 6600 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg 6660 aattctagtg gccggcccag ctgatatcta gacccagctt tcttgtacaa agtggccgtt 6720 aacggatcgg ccagaatggc ccggaccggg ttaccgaatt cgagctcggt accctgggat 6780 ccgatatcga tgggccctgg ccgaagcttg gtcacccggt ccgggcctag aaggccagct 6840 tcggccgccc cgggcaactt tattatacaa agttgatggt acggatccgt cgacggcgcg 6900 ccgcggacct gaattccagc acactggcgg ccgttagtct aaaaacggcc cctaactaac 6960 gcttagttgc ccgctagatt agtcccccgt tagtgcctag ttagacacac gacgaactag 7020 gggctactag taacggccgc cagtgtgctc ggtacggcca gaatggccat ctggattcag 7080 cagggggtga ttgcggttac atcatgtacg gaaaaataat tctaatcctt gatttaaatt 7140 tgaacttgac tatttattta ttctttattt cattttgtaa atcattttat gtatctcctg 7200 gcaagcaatt ttatccacct tgcaccaaca ccttcgggtt ccataatcaa accaccttaa 7260 cttcacacca tgctgtaact cacaccgccc agcatctcca atgtgaaaga agctaaaatt 7320 taataaacaa tcatacgaag cagtgacaaa ataccagatg gtattaatgc ttcgataaaa 7380 ttaattggaa agtataaaat ggtagaaaat aataaattat aattaattta agtaagataa 7440 aaaataatta aaaactaaaa tgttaaaatt ttaaaaaaat tattttaaat aatatttaaa 7500 aacattaaaa atcattttaa aaaatttatt tatagaacaa ttaaataaat atttcagcta 7560 ataaaaaaca aaagcttacc tagccttaga agacaacttg tccaacaatt agatgatacc 7620 cattgccctt acgttttctt taacatcaat tattgttttt gtcaacaagc tatcttttag 7680 ttttatttta ttggtaaaaa atatgtcgcc ttcaagttgc atcatttaac acatctcgtc 7740 attagaaaaa taaaactctt ccctaaacga ttagtagaaa aaatcattcg ataataaata 7800 agaaagaaaa attagaaaaa aataacttca ttttaaaaaa atcattaagg ctatattttt 7860 taaatgacta attttatata gactgtaact aaaagtatac aatttattat gctatgtatc 7920 ttaaagaatt acttataaaa atctacggaa gaatatctta caaagtgaaa aacaaatgag 7980 aaagaattta gtgggatgat tatgatttta tttgaaaatt gaaaaaataa ttattaaaga 8040 ctttagtgga gtaagaaagc tttcctatta gtcttttctt atccataaaa aaaaaaaaaa 8100 aaatctagcg tgacagcttt tccatagatt ttaataatgt aaaatactgg tagcagccga 8160 ccgttcaggt aatggacact gtggtcctaa cttgcaacgg gtgcgggccc aatttaataa 8220 cgccgtggta acggataaag ccaagcgtga agcggtgaag gtacatctct gactccgtca 8280 agattacgaa accgtcaact acgaaggact ccccgaaata tcatctgtgt cataaacacc 8340 aagtcacacc atacatgggc acgcgtcaca atatgattgg agaacggttc caccgcatat 8400 gctataaaat gcccccacac ccctcgaccc taatcgcact tcaattgcaa tcaaattagt 8460 tcattctctt tgcgcagttc cctacctctc ctttcaaggt tcgtagattt cttccgtttt 8520 tttttcttct tctttattgt ttgttctaca tcagcatgat gttgatttga ttgtgttttc 8580 tatcgtttca tcgattataa attttcataa tcagaagatt cagcttttat taatgcaaga 8640 acgtccttaa ttgatgattt tataaccgta aattaggtct aattagagtt tttttcataa 8700 agattttcag atccgtttac aacaagcctt aattgttgat tctgtagtcg tagattaagg 8760 tttttttcat gaactacttc agatccgtta aacaacagcc ttatttgttg atacttcagt 8820 cgtttttcaa gaaattgttc agatccgttg ataaaagcct tattcgttga ttctgtatgg 8880 tatttcaaga gatattgctc aggtccttta gcaactacct tatttgttga ttctgtggcc 8940 atagattagg attttttttc acgaaattgc ttcttgaaat tacgtgatgg attttgattc 9000 tgatttatct tgtgattgtt gactctacag ccatggctgc aactactctt acatctgctt 9060 tacctggtgc tttcagttca tcccagagac caagtgctcc tttcaactta caaagatcac 9120 ctagagtgct tagaaggttc aacagaaaga caggacgtca acctcgtgga cttgttcgtg 9180 ctgcaaaggc tcaagcggcg caaaacctgc cagagaaatt cgatgaaaca agactctggg 9240 aggccctcag gggatttggg gttagtccaa gtagggttac ttacgctccc gtcggattcg 9300 gagattacca ctggaccgtc acagatgaag acgggagacc ttggttcgca actgtcagtg 9360 acctcgaaca caaagagcat tgcgggcagg gagcacaagc agcccttaaa ggactcagac 9420 aagctatgga cactgccctc gcacttagag atagggacgg actcagattc gtggttgctc 9480 cagtggcagc aacagatggg ggtggccctg tccttcccct tgacgctagg tatgcactca 9540 cagtcttccc tcacgtccca ggaagaacag gagagttcgg acaaagactc acagaggcag 9600 agagggacag actgcttgct cttctggcag aactccacgg aagaacacct cctgaaacaa 9660 ccccaccagc cgatatggaa ccaccaggac ttcctggtgt tagagcagca ctcgccgaaa 9720 gtgaaggacc ttggagtgga ggaccatttg cagaacccgc tagacttctt ctcagagaac 9780 acgaggccac actgcacgca agactcgctg aattcgaagc cctcgtcgca agagttaagg 9840 gtcgtggagc acctcttgtc gttacccacg gagaaccaca cccaggcaac ttgatcctcg 9900 gagaggaagg ctatctcctc gtggactggg atacagttgg actggctcca gctgaaaggg 9960 atttgagtct catctcagac gacccagctg acctcgcaag atacgcagag cttacaggaa 10020 gaactccaga cccagacgca ctcgcactct atagactgcg ttggagtctc cttgatgtgg 10080 ctgagttcgt cgagtggttc agggctgaac accaaagaac ccaagacacc gagagtgcat 10140 ggaaggggtt cactgacaca cttgggcaat tggctgctgg tggatcagct gcatagcaaa 10200 cttatctctg ttatgaatca gaagaagttc atgtctcgtt tcatttaaaa ctttggtggt 10260 ttgtgttttg gggccttgta aagcccctga tgaataattg ttcaactatg tttccgttcc 10320 tgtgttatac ctttctttct aatgagtaat gacatcaaac ttcttctgta ttgaaattat 10380 gtccttgtga gtctctttat catcgtttcg tctttacatt atatgtgcta cttttgtcta 10440 atgagcctga aaagtggctc caatggtacg cactggaaga tttgttggct tctggtagat 10500 atagcgacag tgttgagctt gtaatatcat gtctcttatt gctaaattag ttcctttctt 10560 aacagaaacc ttcaaagttt ttgtttttgt tttcatttac ctaatgtaca catacgctgg 10620 ccatgactaa caacatgtcc aggcttagag catatttttt tctagcttaa attgttaact 10680 tgtcattcag taaaatccga gaattgtgaa gctctaattg aagctaattc gttttataaa 10740 gtcagttaaa aagtatacta aattatccaa cttttcttca aaatctcaaa attctatgac 10800 aaaacgatag tctttgttta tgtcagtacc acaaagaggt ggaaaaaaac accaaaaaaa 10860 caataagcaa actatacact gagaagaaaa ataaaagaga gctcaataga tgttttatac 10920 taacggtaga ttagatcaaa gatccaagct ttactctaca tagagcagaa cccagaatcc 10980 cttcatatct cttttattct agcaccgata atctactgaa aagaagacac ttagagctct 11040 gtctctttgt caaagaagtc ccagccgtca tccagaagct ccttacgttc attaacagag 11100 gccgcaattg gatcgggttt acttattttg tgggtatcta tacttttatt agatttttaa 11160 tcaggctcct gatttctttt tatttcgatt gaattcctga acttgtatta ttcagtagat 11220 cgaataaatt ataaaaagat aaaatcataa aataatattt tatcctatca atcatattaa 11280 agcaatgaat atgtaaaatt aatcttatct ttattttaaa aaatcatata ggtttagtat 11340 ttttttaaaa ataaagatag gattagtttt actattcact gcttattact tttaaaaaaa 11400 tcataaaggt ttagtatttt tttaaaataa atataggaat agttttacta ttcactgctt 11460 taatagaaaa atagtttaaa atttaagata gttttaatcc cagcatttgc cacgtttgaa 11520 cgtgagccga aacgatgtcg ttacattatc ttaacctagc tgaaacgatg tcgtcataat 11580 atcgccaaat gccaactgga ctacgtcgaa cccacaaatc ccacaaagcg cgtgaaatca 11640 aatcgctcaa accacaaaaa agaacaacgc gtttgttaca cgctcaatcc cacgcgagta 11700 gagcacagta accttcaaat aagcgaatgg ggcataatca gaaatccgaa ataaacctag 11760 gggcattatc ggaaatgaaa agtagctcac tcaatataaa aatctaggaa ccctagtttt 11820 cgttatcact ctgtgctccc tcgctctatt tctcagtctc tgtgtttgcg gctgaggatt 11880 ccgaacgagt gaccttcttc gtttctcgca aaggtaacag cctctgctct tgtctcttcg 11940 attcgatcta tgcctgtctc ttatttacga tgatgtttct tcggttatgt ttttttattt 12000 atgctttatg ctgttgatgt tcggttgttt gtttcgcttt gtttttgtgg ttcagttttt 12060 taggattctt ttggtttttg aatcgattaa tcggaagaga ttttcgagtt atttggtgtg 12120 ttggaggtga atcttttttt tgaggtcata gatctgttgt atttgtgtta taaacatgcg 12180 actttgtatg attttttacg aggttatgat gttctggttg ttttattatg aatctgttga 12240 gacagaacca tgatttttgt tgatgttcgt ttacactatt aaaggtttgt tttaacagga 12300 ttaaaagttt tttaagcatg ttgaaggagt cttgtagata tgtaaccgtc gatagttttt 12360 ttgtgggttt gttcacatgt tatcaagctt aatcttttac tatgtatgcg accatatctg 12420 gatccagcaa aggcgatttt ttaattcctt gtgaaacttt tgtaatatga agttgaaatt 12480 ttgttattgg taaactataa atgtgtgaag ttggagtata cctttacctt cttatttggc 12540 tttgtgatag tttaatttat atgtattttg agttctgact tgtatttctt tgaattgatt 12600 ctagtttaag taatcatggc ctcctccgag aacgtcatca ccgagttcat gcgcttcaag 12660 gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 12720 cgcccctacg agggccacaa caccgtgaag ctgaaggtga cgaagggcgg ccccctgccc 12780 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac 12840 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc 12900 gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc aggactcctc cctgcaggac 12960 ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga cggccccgtg 13020 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 13080 gtgctgaagg gcgagaccca caaggccctg aagctgaagg acggcggcca ctacctggtg 13140 gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg 13200 gacgccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag 13260 cgcaccgagg gccgccacca cctgttcctg tagttttgtg atctgatgat aagtggttgg 13320 ttcgtgtctc atgcacttgg gaggtgatct atttcacctg gtgtagtttg tgtttccgtc 13380 agttggaaaa acttatccct atcgatttcg ttttcatttt ctgcttttct tttatgtacc 13440 ttcgtttggg cttgtaacgg gcctttgtat ttcaactctc aataataatc caagtgcatg 13500 ttaaacaatt tgtcatctgt ttcggctttg atatactact ggtgaagatg ggccgtacta 13560 ctgcatcaca acgaaaaata ataataagat gaaaaacttg aagtggaaaa aaaaaaaaac 13620 ttgaatgttc actactactc attgaccata atgtttaaca tacatagctc aatagtattt 13680 ttgtgaatat ggcaacacaa acagtccaaa acaattgtct cttactatac caaaccaagg 13740 gcgccgcttg tttgccactc tttgtgtgca atagtgtgat taccacatct ccacattcaa 13800 tatattccct gaattatctg acgattttga tggctcactg ttttcccaag tcttgaattg 13860 tcttctgtgc gccagtcaaa tgcatatgtg ttgagtttat cttttaaata tcaagctttt 13920 gtttttaact tttgtttgta accaaaaact cacagtagga gtttgatcac ataattttat 13980 gtttgccttt gcaatttcta gtgagtcttt gattaaaagc ttgaaaagaa aatgcagcca 14040 agcttaccaa gtaagttatg tgtattaacc agaggaagag agaatcttgc aaaatttcaa 14100 caaacacaaa aagaagtatt actacgattg gtggagaaag aaaacgattc caaatcttga 14160 actgttgttg taaaagcata gcagaaagtg ggagacaacc gaaatagaaa tgactataac 14220 ttaatttaat gttatcatta taatttcttc tagcaaatat ttagaaagta aatatcacat 14280 caacctttaa tgtaattaag ctttctcttt ttgattcatg tgagatgaaa agaaaaaaaa 14340 gaagagaaaa gtgtagaaaa cacatcattt ctaagctgaa gctgaggtac aaattaatta 14400 ataagtgact agggtcacgt gaccctagtc acttatgatt ccgatgactt cgtaggttcc 14460 tagctcaagc cgctcgtgtc caagcgtcac ttacgattag ctaatgatta cggcatctag 14520 gaccgactag ttcttatgtg cttctagtct ccaaatgtgg ttgatagtta ttttgctcta 14580 agatcaacag taatgaagta taaatcatcg ttgtggtgtg ctactcggtt aattgagcat 14640 taacacacac aaacatgacg aggatggtat aatctccaaa aatgtgtact ttgttaggtg 14700 ggaccctata gccttgatta atgtgctatg ttaggcatgc ctggaaacgt gtgacgcata 14760 tgttttgtga acctgttgat attatatgtg cttttatatt accatatttt attaaaatac 14820 taatatttat tactagtaag atataacatt ctatctagct taaaaactaa ccataaatat 14880 tccataataa ctagatttac caaactaata tactaaatat acataataaa tacaaaatta 14940 acaagacaat aatcaatatt tatgagctta atatatttag acattatggt tggtcgacga 15000 taatcatgct aacttttcgt aattgcttga ttgaaatatg cttagaataa tgcctctttg 15060 ttctacatgg caaataggga ccattatggt gtaacaccct gggaaccaca aacaccccga 15120 aatgctacta aactacacaa ctaaccttca tatataaaat ttcgacagca tctcctttga 15180 aaatttgcat agacgtggaa gcaacagagt ataaacagat ctagctagct aagtttaaac 15240 ggccggccaa acgtgcaagc gctactagac aattcagtac attaaaaacg tccgcaatgt 15300 gttattaagt tgtctaagcg tcaatttatt tacaattgaa tatatcctgc ca 15352 <210> 156 <211> 15433 <212> DNA <213> Artificial Sequence <220> <223> RV026534 T-DNA <400> 156 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaaacagcac agcaccagca gaacaaccaa cagcactacc tccaccaaca cctctccatc 1860 ggacaaggaa caaacatcga ggacggcagt aacaagaaca actcctccaa cttcatgtgc 1920 aggcaaaata gtacccgctg gacaccaacc actgaccaga tcaggatatt aaaggacctc 1980 tactacaaca acggagtcag atcacctacc gctgaacaga tccagagaat ctccgccaag 2040 ctcaggcagt acggcaagat cgagggcaag aacgtcttct actggttcca gaaccataaa 2100 gcccgcgaga gacaaaagaa gcgcctcatc gccgcagcca ccaccgataa cactaacctc 2160 ccaatgcaga tgcagttcca gagaggagtc tggagatcct ctgcagacga ccccatccac 2220 cacaagtata ctaatccagg agtgcactgc ccttcagctt cttcccacgg agttctcgca 2280 gtcggacaga acggcaatca cggatacgga gccttggcaa tggaaaaatc cttccgcgac 2340 tgctccatca gtccaggcag ttccatgtca caccaccacc accaaaactt cgcttgggca 2400 ggagtcgatc cttattcctc aaccacaacc taccctttcc tcgagaagac caagcacttc 2460 gagaatgaaa ccctcgaagc tgatgaagag cagcaagagg aggaccagga gaactactac 2520 taccaaagga ccacctctgc catcgaaacc ctgcctctct tccctatgca cgaggagaac 2580 atctcctcct tctgcaacct caagcaccag gagtcatccg gaggattcta caccgagtgg 2640 tacagagcag acgacaacct cgcagcagcc agagcttccc tcgagctctc cttgaactcc 2700 ttcatcggca actcctccta gggtacctga catcttatag tctgcaacct ctcgtgtctg 2760 aattcctatc tttatcaagt gttattgctt ccacgactat aggacagctt tcgtcgaaag 2820 gttttgctca tgtgatctcg aaggattcat ctagtctgat ttttcgtgac ttgtatcggt 2880 tttattggat tcatccaaca tatatcaata aaaaatgagt tgtgtttcct ttcttcctag 2940 ttcagttaaa attatttccc tcctgcgctt gtgctgtaat tgtctgtgta cctgttgttt 3000 gtgactgtgt tagttccctt ggatatgatt tcgtatttga tatgtacatg gagatagctt 3060 agcttcatta ttggagtatg aagttagtat gacatagtca ctctcctgga aaattgacac 3120 tgcaaaccat atttttattc tgaaccacaa atcctagtca gtccgctggc atatgccgtc 3180 cgtttgctga atccagaacg tgggtttgga gatgtacggc tgagatgcct ctatgcgaag 3240 gggatttcgt ggtgaaacga gatgggagta gagcaacgcc cgtggaagat gcttcaaact 3300 tccacacttt tgagcaacga tcggcagtag taaggtagac gatttcaaga tcaaagcata 3360 tgaagataaa caacatcaac aacaaaattt gttggggttc tatagagaga aacagagcta 3420 catacataca ctgttttgta tctaccatct gagatgatga aaagatgaaa aactaaagaa 3480 tgccccggcg ccaacgccag gacacgccgc gcgcgcgtca cccgagccat ctcttgaccc 3540 agccggcgct gtatatttac acacgttgca gcatcgatca ccacctgttc gatcgcgtcg 3600 ccgtcaccaa gcttggcgcg ccggtcaccc ggtccgggcc tagaaggcca cccggggctc 3660 ggcgttaggc ttttagtccg ctaggtggct agaccgaggt taggacgcta gttcactagg 3720 ggcctagtgg tgttttaagg atgggcaagt ttgtacaaaa aagcaggctg gcgccggaac 3780 caattcagtc gagtgcggcc gccagtgtga tggatatctc gacccgggtg attgcggtta 3840 catcatgtac ggaaaaataa ttctaatcct tgatttaaat ttgaacttga ctatttattt 3900 attctttatt tcattttgta aatcatttta tgtatctcct ggcaagcaat tttatccacc 3960 ttgcaccaac accttcgggt tccataatca aaccacctta acttcacacc atgctgtaac 4020 tcacaccgcc cagcatctcc aatgtgaaag aagctaaaat ttaataaaca atcatacgaa 4080 gcagtgacaa aataccagat ggtattaatg cttcgataaa attaattgga aagtataaaa 4140 tggtagaaaa taataaatta taattaattt aagtaagata aaaaataatt aaaaactaaa 4200 atgttaaaat tttaaaaaaa ttattttaaa taatatttaa aaacattaaa aatcatttta 4260 aaaaatttat ttatagaaca attaaataaa tatttcagct aataaaaaac aaaagcttac 4320 ctagccttag aagacaactt gtccaacaat tagatgatac ccattgccct tacgttttct 4380 ttaacatcaa ttattgtttt tgtcaacaag ctatctttta gttttatttt attggtaaaa 4440 aatatgtcgc cttcaagttg catcatttaa cacatctcgt cattagaaaa ataaaactct 4500 tccctaaacg attagtagaa aaaatcattc gataataaat aagaaagaaa aattagaaaa 4560 aaataacttc attttaaaaa aatcattaag gctatatttt ttaaatgact aattttatat 4620 agactgtaac taaaagtata caatttatta tgctatgtat cttaaagaat tacttataaa 4680 aatctacgga agaatatctt acaaagtgaa aaacaaatga gaaagaattt agtgggatga 4740 ttatgatttt atttgaaaat tgaaaaaata attattaaag actttagtgg agtaagaaag 4800 ctttcctatt agtcttttct tatccataaa aaaaaaaaaa aaaatctagc gtgacagctt 4860 ttccatagat tttaataatg taaaatactg gtagcagccg accgttcagg taatggacac 4920 tgtggtccta acttgcaacg ggtgcgggcc caatttaata acgccgtggt aacggataaa 4980 gccaagcgtg aagcggtgaa ggtacatctc tgactccgtc aagattacga aaccgtcaac 5040 tacgaaggac tccccgaaat atcatctgtg tcataaacac caagtcacac catacatggg 5100 cacgcgtcac aatatgattg gagaacggtt ccaccgcata tgctataaaa tgcccccaca 5160 cccctcgacc ctaatcgcac ttcaattgca atcaaattag ttcattctct ttgcgcagtt 5220 ccctacctct cctttcaagg ttcgtagatt tcttccgttt ttttttcttc ttctttattg 5280 tttgttctac atcagcatga tgttgatttg attgtgtttt ctatcgtttc atcgattata 5340 aattttcata atcagaagat tcagctttta ttaatgcaag aacgtcctta attgatgatt 5400 ttataaccgt aaattaggtc taattagagt ttttttcata aagattttca gatccgttta 5460 caacaagcct taattgttga ttctgtagtc gtagattaag gtttttttca tgaactactt 5520 cagatccgtt aaacaacagc cttatttgtt gatacttcag tcgtttttca agaaattgtt 5580 cagatccgtt gataaaagcc ttattcgttg attctgtatg gtatttcaag agatattgct 5640 caggtccttt agcaactacc ttatttgttg attctgtggc catagattag gatttttttt 5700 cacgaaattg cttcttgaaa ttacgtgatg gattttgatt ctgatttatc ttgtgattgt 5760 tgactctaca gccatggccc acagcaagca cggcctgaag gaggagatga ccatgaagta 5820 ccacatggag ggctgcgtga acggccacaa gttcgtgatc accggcgagg gcatcggcta 5880 ccccttcaag ggcaagcaga ccatcaacct gtgcgtgatc gagggcggcc ccctgccctt 5940 cagcgaggac atcctgagcg ccggcttcaa gtacggcgac cggatcttca ccgagtaccc 6000 ccaggacatc gtggactact tcaagaacag ctgccccgcc ggctacacct ggggccggag 6060 cttcctgttc gaggacggcg ccgtgtgcat ctgtaacgtg gacatcaccg tgagcgtgaa 6120 ggagaactgc atctaccaca agagcatctt caacggcgtg aacttccccg ccgacggccc 6180 cgtgatgaag aagatgacca ccaactggga ggccagctgc gagaagatca tgcccgtgcc 6240 taagcagggc atcctgaagg gcgacgtgag catgtacctg ctgctgaagg acggcggccg 6300 gtaccggtgc cagttcgaca ccgtgtacaa ggccaagagc gtgcccagca agatgcccga 6360 gtggcacttc atccagcaca agctgctgcg ggaggaccgg agcgacgcca agaaccagaa 6420 gtggcagctg accgagcacg ccatcgcctt ccccagcgcc ctggcctgag agctcgaatt 6480 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc 6540 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt 6600 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt 6660 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt 6720 catctatgtt actagatcgg gaattctagt ggccggccca gctgatatct agacccagct 6780 ttcttgtaca aagtggccgt taacggatcg gccagaatgg cccggaccgg gttaccgaat 6840 tcgagctcgg taccctggga tccgatatcg atgggccctg gccgaagctt ggtcacccgg 6900 tccgggccta gaaggccagc ttcggccgcc ccgggcaact ttattataca aagttgatgg 6960 tacggatccg tcgacggcgc gccgcggacc tgaattccag cacactggcg gccgttagtc 7020 taaaaacggc ccctaactaa cgcttagttg cccgctagat tagtcccccg ttagtgccta 7080 gttagacaca cgacgaacta ggggctacta gtaacggccg ccagtgtgct cggtacggcc 7140 agaatggcca tctggattca gcagggggtg attgcggtta catcatgtac ggaaaaataa 7200 ttctaatcct tgatttaaat ttgaacttga ctatttattt attctttatt tcattttgta 7260 aatcatttta tgtatctcct ggcaagcaat tttatccacc ttgcaccaac accttcgggt 7320 tccataatca aaccacctta acttcacacc atgctgtaac tcacaccgcc cagcatctcc 7380 aatgtgaaag aagctaaaat ttaataaaca atcatacgaa gcagtgacaa aataccagat 7440 ggtattaatg cttcgataaa attaattgga aagtataaaa tggtagaaaa taataaatta 7500 taattaattt aagtaagata aaaaataatt aaaaactaaa atgttaaaat tttaaaaaaa 7560 ttattttaaa taatatttaa aaacattaaa aatcatttta aaaaatttat ttatagaaca 7620 attaaataaa tatttcagct aataaaaaac aaaagcttac ctagccttag aagacaactt 7680 gtccaacaat tagatgatac ccattgccct tacgttttct ttaacatcaa ttattgtttt 7740 tgtcaacaag ctatctttta gttttatttt attggtaaaa aatatgtcgc cttcaagttg 7800 catcatttaa cacatctcgt cattagaaaa ataaaactct tccctaaacg attagtagaa 7860 aaaatcattc gataataaat aagaaagaaa aattagaaaa aaataacttc attttaaaaa 7920 aatcattaag gctatatttt ttaaatgact aattttatat agactgtaac taaaagtata 7980 caatttatta tgctatgtat cttaaagaat tacttataaa aatctacgga agaatatctt 8040 acaaagtgaa aaacaaatga gaaagaattt agtgggatga ttatgatttt atttgaaaat 8100 tgaaaaaata attattaaag actttagtgg agtaagaaag ctttcctatt agtcttttct 8160 tatccataaa aaaaaaaaaa aaaatctagc gtgacagctt ttccatagat tttaataatg 8220 taaaatactg gtagcagccg accgttcagg taatggacac tgtggtccta acttgcaacg 8280 ggtgcgggcc caatttaata acgccgtggt aacggataaa gccaagcgtg aagcggtgaa 8340 ggtacatctc tgactccgtc aagattacga aaccgtcaac tacgaaggac tccccgaaat 8400 atcatctgtg tcataaacac caagtcacac catacatggg cacgcgtcac aatatgattg 8460 gagaacggtt ccaccgcata tgctataaaa tgcccccaca cccctcgacc ctaatcgcac 8520 ttcaattgca atcaaattag ttcattctct ttgcgcagtt ccctacctct cctttcaagg 8580 ttcgtagatt tcttccgttt ttttttcttc ttctttattg tttgttctac atcagcatga 8640 tgttgatttg attgtgtttt ctatcgtttc atcgattata aattttcata atcagaagat 8700 tcagctttta ttaatgcaag aacgtcctta attgatgatt ttataaccgt aaattaggtc 8760 taattagagt ttttttcata aagattttca gatccgttta caacaagcct taattgttga 8820 ttctgtagtc gtagattaag gtttttttca tgaactactt cagatccgtt aaacaacagc 8880 cttatttgtt gatacttcag tcgtttttca agaaattgtt cagatccgtt gataaaagcc 8940 ttattcgttg attctgtatg gtatttcaag agatattgct caggtccttt agcaactacc 9000 ttatttgttg attctgtggc catagattag gatttttttt cacgaaattg cttcttgaaa 9060 ttacgtgatg gattttgatt ctgatttatc ttgtgattgt tgactctaca gccatggctg 9120 caactactct tacatctgct ttacctggtg ctttcagttc atcccagaga ccaagtgctc 9180 ctttcaactt acaaagatca cctagagtgc ttagaaggtt caacagaaag acaggacgtc 9240 aacctcgtgg acttgttcgt gctgcaaagg ctcaagcggc gcaaaacctg ccagagaaat 9300 tcgatgaaac aagactctgg gaggccctca ggggatttgg ggttagtcca agtagggtta 9360 cttacgctcc cgtcggattc ggagattacc actggaccgt cacagatgaa gacgggagac 9420 cttggttcgc aactgtcagt gacctcgaac acaaagagca ttgcgggcag ggagcacaag 9480 cagcccttaa aggactcaga caagctatgg acactgccct cgcacttaga gatagggacg 9540 gactcagatt cgtggttgct ccagtggcag caacagatgg gggtggccct gtccttcccc 9600 ttgacgctag gtatgcactc acagtcttcc ctcacgtccc aggaagaaca ggagagttcg 9660 gacaaagact cacagaggca gagagggaca gactgcttgc tcttctggca gaactccacg 9720 gaagaacacc tcctgaaaca accccaccag ccgatatgga accaccagga cttcctggtg 9780 ttagagcagc actcgccgaa agtgaaggac cttggagtgg aggaccattt gcagaacccg 9840 ctagacttct tctcagagaa cacgaggcca cactgcacgc aagactcgct gaattcgaag 9900 ccctcgtcgc aagagttaag ggtcgtggag cacctcttgt cgttacccac ggagaaccac 9960 acccaggcaa cttgatcctc ggagaggaag gctatctcct cgtggactgg gatacagttg 10020 gactggctcc agctgaaagg gatttgagtc tcatctcaga cgacccagct gacctcgcaa 10080 gatacgcaga gcttacagga agaactccag acccagacgc actcgcactc tatagactgc 10140 gttggagtct ccttgatgtg gctgagttcg tcgagtggtt cagggctgaa caccaaagaa 10200 cccaagacac cgagagtgca tggaaggggt tcactgacac acttgggcaa ttggctgctg 10260 gtggatcagc tgcatagcaa acttatctct gttatgaatc agaagaagtt catgtctcgt 10320 ttcatttaaa actttggtgg tttgtgtttt ggggccttgt aaagcccctg atgaataatt 10380 gttcaactat gtttccgttc ctgtgttata cctttctttc taatgagtaa tgacatcaaa 10440 cttcttctgt attgaaatta tgtccttgtg agtctcttta tcatcgtttc gtctttacat 10500 tatatgtgct acttttgtct aatgagcctg aaaagtggct ccaatggtac gcactggaag 10560 atttgttggc ttctggtaga tatagcgaca gtgttgagct tgtaatatca tgtctcttat 10620 tgctaaatta gttcctttct taacagaaac cttcaaagtt tttgtttttg ttttcattta 10680 cctaatgtac acatacgctg gccatgacta acaacatgtc caggcttaga gcatattttt 10740 ttctagctta aattgttaac ttgtcattca gtaaaatccg agaattgtga agctctaatt 10800 gaagctaatt cgttttataa agtcagttaa aaagtatact aaattatcca acttttcttc 10860 aaaatctcaa aattctatga caaaacgata gtctttgttt atgtcagtac cacaaagagg 10920 tggaaaaaaa caccaaaaaa acaataagca aactatacac tgagaagaaa aataaaagag 10980 agctcaatag atgttttata ctaacggtag attagatcaa agatccaagc tttactctac 11040 atagagcaga acccagaatc ccttcatatc tcttttattc tagcaccgat aatctactga 11100 aaagaagaca cttagagctc tgtctctttg tcaaagaagt cccagccgtc atccagaagc 11160 tccttacgtt cattaacaga ggccgcaatt ggatcgggtt tacttatttt gtgggtatct 11220 atacttttat tagattttta atcaggctcc tgatttcttt ttatttcgat tgaattcctg 11280 aacttgtatt attcagtaga tcgaataaat tataaaaaga taaaatcata aaataatatt 11340 ttatcctatc aatcatatta aagcaatgaa tatgtaaaat taatcttatc tttattttaa 11400 aaaatcatat aggtttagta tttttttaaa aataaagata ggattagttt tactattcac 11460 tgcttattac ttttaaaaaa atcataaagg tttagtattt ttttaaaata aatataggaa 11520 tagttttact attcactgct ttaatagaaa aatagtttaa aatttaagat agttttaatc 11580 ccagcatttg ccacgtttga acgtgagccg aaacgatgtc gttacattat cttaacctag 11640 ctgaaacgat gtcgtcataa tatcgccaaa tgccaactgg actacgtcga acccacaaat 11700 cccacaaagc gcgtgaaatc aaatcgctca aaccacaaaa aagaacaacg cgtttgttac 11760 acgctcaatc ccacgcgagt agagcacagt aaccttcaaa taagcgaatg gggcataatc 11820 agaaatccga aataaaccta ggggcattat cggaaatgaa aagtagctca ctcaatataa 11880 aaatctagga accctagttt tcgttatcac tctgtgctcc ctcgctctat ttctcagtct 11940 ctgtgtttgc ggctgaggat tccgaacgag tgaccttctt cgtttctcgc aaaggtaaca 12000 gcctctgctc ttgtctcttc gattcgatct atgcctgtct cttatttacg atgatgtttc 12060 ttcggttatg tttttttatt tatgctttat gctgttgatg ttcggttgtt tgtttcgctt 12120 tgtttttgtg gttcagtttt ttaggattct tttggttttt gaatcgatta atcggaagag 12180 attttcgagt tatttggtgt gttggaggtg aatctttttt ttgaggtcat agatctgttg 12240 tatttgtgtt ataaacatgc gactttgtat gattttttac gaggttatga tgttctggtt 12300 gttttattat gaatctgttg agacagaacc atgatttttg ttgatgttcg tttacactat 12360 taaaggtttg ttttaacagg attaaaagtt ttttaagcat gttgaaggag tcttgtagat 12420 atgtaaccgt cgatagtttt tttgtgggtt tgttcacatg ttatcaagct taatctttta 12480 ctatgtatgc gaccatatct ggatccagca aaggcgattt tttaattcct tgtgaaactt 12540 ttgtaatatg aagttgaaat tttgttattg gtaaactata aatgtgtgaa gttggagtat 12600 acctttacct tcttatttgg ctttgtgata gtttaattta tatgtatttt gagttctgac 12660 ttgtatttct ttgaattgat tctagtttaa gtaatcatgg cctcctccga gaacgtcatc 12720 accgagttca tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag 12780 atcgagggcg agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg 12840 acgaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc 12900 tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc 12960 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggc gaccgtgacc 13020 caggactcct ccctgcagga cggctgcttc atctacaagg tgaagttcat cggcgtgaac 13080 ttcccctccg acggccccgt gatgcagaag aagaccatgg gctgggaggc ctccaccgag 13140 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagaccc acaaggccct gaagctgaag 13200 gacggcggcc actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag 13260 ctgcccggct actactacgt ggacgccaag ctggacatca cctcccacaa cgaggactac 13320 accatcgtgg agcagtacga gcgcaccgag ggccgccacc acctgttcct gtagttttgt 13380 gatctgatga taagtggttg gttcgtgtct catgcacttg ggaggtgatc tatttcacct 13440 ggtgtagttt gtgtttccgt cagttggaaa aacttatccc tatcgatttc gttttcattt 13500 tctgcttttc ttttatgtac cttcgtttgg gcttgtaacg ggcctttgta tttcaactct 13560 caataataat ccaagtgcat gttaaacaat ttgtcatctg tttcggcttt gatatactac 13620 tggtgaagat gggccgtact actgcatcac aacgaaaaat aataataaga tgaaaaactt 13680 gaagtggaaa aaaaaaaaaa cttgaatgtt cactactact cattgaccat aatgtttaac 13740 atacatagct caatagtatt tttgtgaata tggcaacaca aacagtccaa aacaattgtc 13800 tcttactata ccaaaccaag ggcgccgctt gtttgccact ctttgtgtgc aatagtgtga 13860 ttaccacatc tccacattca atatattccc tgaattatct gacgattttg atggctcact 13920 gttttcccaa gtcttgaatt gtcttctgtg cgccagtcaa atgcatatgt gttgagttta 13980 tcttttaaat atcaagcttt tgtttttaac ttttgtttgt aaccaaaaac tcacagtagg 14040 agtttgatca cataatttta tgtttgcctt tgcaatttct agtgagtctt tgattaaaag 14100 cttgaaaaga aaatgcagcc aagcttacca agtaagttat gtgtattaac cagaggaaga 14160 gagaatcttg caaaatttca acaaacacaa aaagaagtat tactacgatt ggtggagaaa 14220 gaaaacgatt ccaaatcttg aactgttgtt gtaaaagcat agcagaaagt gggagacaac 14280 cgaaatagaa atgactataa cttaatttaa tgttatcatt ataatttctt ctagcaaata 14340 tttagaaagt aaatatcaca tcaaccttta atgtaattaa gctttctctt tttgattcat 14400 gtgagatgaa aagaaaaaaa agaagagaaa agtgtagaaa acacatcatt tctaagctga 14460 agctgaggta caaattaatt aataagtgac tagggtcacg tgaccctagt cacttatgat 14520 tccgatgact tcgtaggttc ctagctcaag ccgctcgtgt ccaagcgtca cttacgatta 14580 gctaatgatt acggcatcta ggaccgacta gttcttatgt gcttctagtc tccaaatgtg 14640 gttgatagtt attttgctct aagatcaaca gtaatgaagt ataaatcatc gttgtggtgt 14700 gctactcggt taattgagca ttaacacaca caaacatgac gaggatggta taatctccaa 14760 aaatgtgtac tttgttaggt gggaccctat agccttgatt aatgtgctat gttaggcatg 14820 cctggaaacg tgtgacgcat atgttttgtg aacctgttga tattatatgt gcttttatat 14880 taccatattt tattaaaata ctaatattta ttactagtaa gatataacat tctatctagc 14940 ttaaaaacta accataaata ttccataata actagattta ccaaactaat atactaaata 15000 tacataataa atacaaaatt aacaagacaa taatcaatat ttatgagctt aatatattta 15060 gacattatgg ttggtcgacg ataatcatgc taacttttcg taattgcttg attgaaatat 15120 gcttagaata atgcctcttt gttctacatg gcaaataggg accattatgg tgtaacaccc 15180 tgggaaccac aaacaccccg aaatgctact aaactacaca actaaccttc atatataaaa 15240 tttcgacagc atctcctttg aaaatttgca tagacgtgga agcaacagag tataaacaga 15300 tctagctagc taagtttaaa cggccggcca aacgtgcaag cgctactaga caattcagta 15360 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttat ttacaattga 15420 atatatcctg cca 15433 <210> 157 <211> 15458 <212> DNA <213> Artificial Sequence <220> <223> RV026531 T-DNA <400> 157 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccaagc ttggatctcc tgcaggatct ggccggccgg atgggcggat 1320 ctcgtacctc aactttgtat agaaaagttg ggccagaatg gcccggaccg ggttaccaag 1380 cggccgcgaa ttccagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcg 1440 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa 1500 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat 1560 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa 1620 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta 1680 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca 1740 tttcatttgg agaggaccca tggaccctca acagaaccca aatgaagtac aagaaggagg 1800 caacaggcag gggggtggaa tcatgtgcag gcaaagcagt acgcggtgga cacccacaac 1860 tgatcagata aaaatcttga aggaccttta ctacaacaat ggcattaggt cacccagcgc 1920 tgagcagatt cagaggatct ctgctaagct gcgacagttc ggcaagatcg aaggcaagaa 1980 cgttttctat tggtttcaga accacaaagc tcgtgagagg cagaagaaaa gattcacttc 2040 ttcttctgct cctgatcacc atcatcatgc agcaccacca gtgccagctg taccaccaga 2100 atccaataat atcaggcaaa gatcatcagg ggttgggatt gatattaatg caactgctgc 2160 tgctgcttat gaacaagcac ccattaatca ccaccgcaag tattccaaca tttctgctcc 2220 accagccggg ttttcttctg catcttcttc ttcagttggt gtgaatgttt ctgttggggc 2280 acagatggga aactatgggt atggatccat tgctatggag aagagcttta gggagtgctc 2340 aatatcatct ggaggaagta ctagcactgg tcatgtgggt ggatctaatt ataatacttt 2400 taatcacaac tatggatcat gggttggtgt tgatccatat tcctcgccct acactatctt 2460 tgacaagaaa tcctcatcaa aaccagtgtt tggtgaccag gaaaatatga cggaagaaga 2520 atactacaat ctgcaagctt cccaagagat tgagactctc ccactcttcc ccatgcacgg 2580 tgaagacatt catggctttg gcaacatcaa gtcatcctct atggatggct actactccgg 2640 ctggtacagg tccgatggcg gcaacgatgg tggctctcgc acttcccttg agcttagcct 2700 caattcctac ggtcacatga cccatgatta cttcagatca tattgaggta cctgacatct 2760 tatagtctgc aacctctcgt gtctgaattc ctatctttat caagtgttat tgcttccacg 2820 actataggac agctttcgtc gaaaggtttt gctcatgtga tctcgaagga ttcatctagt 2880 ctgatttttc gtgacttgta tcggttttat tggattcatc caacatatat caataaaaaa 2940 tgagttgtgt ttcctttctt cctagttcag ttaaaattat ttccctcctg cgcttgtgct 3000 gtaattgtct gtgtacctgt tgtttgtgac tgtgttagtt cccttggata tgatttcgta 3060 tttgatatgt acatggagat agcttagctt cattattgga gtatgaagtt agtatgacat 3120 agtcactctc ctggaaaatt gacactgcaa accatatttt tattctgaac cacaaatcct 3180 agtcagtccg ctggcatatg ccgtccgttt gctgaatcca gaacgtgggt ttggagatgt 3240 acggctgaga tgcctctatg cgaaggggat ttcgtggtga aacgagatgg gagtagagca 3300 acgcccgtgg aagatgcttc aaacttccac acttttgagc aacgatcggc agtagtaagg 3360 tagacgattt caagatcaaa gcatatgaag ataaacaaca tcaacaacaa aatttgttgg 3420 ggttctatag agagaaacag agctacatac atacactgtt ttgtatctac catctgagat 3480 gatgaaaaga tgaaaaacta aagaatgccc cggcgccaac gccaggacac gccgcgcgcg 3540 cgtcacccga gccatctctt gacccagccg gcgctgtata tttacacacg ttgcagcatc 3600 gatcaccacc tgttcgatcg cgtcgccgtc accaagcttg gcgcgccggt cacccggtcc 3660 gggcctagaa ggccacccgg ggctcggcgt taggctttta gtccgctagg tggctagacc 3720 gaggttagga cgctagttca ctaggggcct agtggtgttt taaggatggg caagtttgta 3780 caaaaaagca ggctggcgcc ggaaccaatt cagtcgagtg cggccgccag tgtgatggat 3840 atctcgaccc gggtgattgc ggttacatca tgtacggaaa aataattcta atccttgatt 3900 taaatttgaa cttgactatt tatttattct ttatttcatt ttgtaaatca ttttatgtat 3960 ctcctggcaa gcaattttat ccaccttgca ccaacacctt cgggttccat aatcaaacca 4020 ccttaacttc acaccatgct gtaactcaca ccgcccagca tctccaatgt gaaagaagct 4080 aaaatttaat aaacaatcat acgaagcagt gacaaaatac cagatggtat taatgcttcg 4140 ataaaattaa ttggaaagta taaaatggta gaaaataata aattataatt aatttaagta 4200 agataaaaaa taattaaaaa ctaaaatgtt aaaattttaa aaaaattatt ttaaataata 4260 tttaaaaaca ttaaaaatca ttttaaaaaa tttatttata gaacaattaa ataaatattt 4320 cagctaataa aaaacaaaag cttacctagc cttagaagac aacttgtcca acaattagat 4380 gatacccatt gcccttacgt tttctttaac atcaattatt gtttttgtca acaagctatc 4440 ttttagtttt attttattgg taaaaaatat gtcgccttca agttgcatca tttaacacat 4500 ctcgtcatta gaaaaataaa actcttccct aaacgattag tagaaaaaat cattcgataa 4560 taaataagaa agaaaaatta gaaaaaaata acttcatttt aaaaaaatca ttaaggctat 4620 attttttaaa tgactaattt tatatagact gtaactaaaa gtatacaatt tattatgcta 4680 tgtatcttaa agaattactt ataaaaatct acggaagaat atcttacaaa gtgaaaaaca 4740 aatgagaaag aatttagtgg gatgattatg attttatttg aaaattgaaa aaataattat 4800 taaagacttt agtggagtaa gaaagctttc ctattagtct tttcttatcc ataaaaaaaa 4860 aaaaaaaaat ctagcgtgac agcttttcca tagattttaa taatgtaaaa tactggtagc 4920 agccgaccgt tcaggtaatg gacactgtgg tcctaacttg caacgggtgc gggcccaatt 4980 taataacgcc gtggtaacgg ataaagccaa gcgtgaagcg gtgaaggtac atctctgact 5040 ccgtcaagat tacgaaaccg tcaactacga aggactcccc gaaatatcat ctgtgtcata 5100 aacaccaagt cacaccatac atgggcacgc gtcacaatat gattggagaa cggttccacc 5160 gcatatgcta taaaatgccc ccacacccct cgaccctaat cgcacttcaa ttgcaatcaa 5220 attagttcat tctctttgcg cagttcccta cctctccttt caaggttcgt agatttcttc 5280 cgtttttttt tcttcttctt tattgtttgt tctacatcag catgatgttg atttgattgt 5340 gttttctatc gtttcatcga ttataaattt tcataatcag aagattcagc ttttattaat 5400 gcaagaacgt ccttaattga tgattttata accgtaaatt aggtctaatt agagtttttt 5460 tcataaagat tttcagatcc gtttacaaca agccttaatt gttgattctg tagtcgtaga 5520 ttaaggtttt tttcatgaac tacttcagat ccgttaaaca acagccttat ttgttgatac 5580 ttcagtcgtt tttcaagaaa ttgttcagat ccgttgataa aagccttatt cgttgattct 5640 gtatggtatt tcaagagata ttgctcaggt cctttagcaa ctaccttatt tgttgattct 5700 gtggccatag attaggattt tttttcacga aattgcttct tgaaattacg tgatggattt 5760 tgattctgat ttatcttgtg attgttgact ctacagccat ggcccacagc aagcacggcc 5820 tgaaggagga gatgaccatg aagtaccaca tggagggctg cgtgaacggc cacaagttcg 5880 tgatcaccgg cgagggcatc ggctacccct tcaagggcaa gcagaccatc aacctgtgcg 5940 tgatcgaggg cggccccctg cccttcagcg aggacatcct gagcgccggc ttcaagtacg 6000 gcgaccggat cttcaccgag tacccccagg acatcgtgga ctacttcaag aacagctgcc 6060 ccgccggcta cacctggggc cggagcttcc tgttcgagga cggcgccgtg tgcatctgta 6120 acgtggacat caccgtgagc gtgaaggaga actgcatcta ccacaagagc atcttcaacg 6180 gcgtgaactt ccccgccgac ggccccgtga tgaagaagat gaccaccaac tgggaggcca 6240 gctgcgagaa gatcatgccc gtgcctaagc agggcatcct gaagggcgac gtgagcatgt 6300 acctgctgct gaaggacggc ggccggtacc ggtgccagtt cgacaccgtg tacaaggcca 6360 agagcgtgcc cagcaagatg cccgagtggc acttcatcca gcacaagctg ctgcgggagg 6420 accggagcga cgccaagaac cagaagtggc agctgaccga gcacgccatc gccttcccca 6480 gcgccctggc ctgagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc 6540 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 6600 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 6660 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 6720 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ctagtggccg 6780 gcccagctga tatctagacc cagctttctt gtacaaagtg gccgttaacg gatcggccag 6840 aatggcccgg accgggttac cgaattcgag ctcggtaccc tgggatccga tatcgatggg 6900 ccctggccga agcttggtca cccggtccgg gcctagaagg ccagcttcgg ccgccccggg 6960 caactttatt atacaaagtt gatggtacgg atccgtcgac ggcgcgccgc ggacctgaat 7020 tccagcacac tggcggccgt tagtctaaaa acggccccta actaacgctt agttgcccgc 7080 tagattagtc ccccgttagt gcctagttag acacacgacg aactaggggc tactagtaac 7140 ggccgccagt gtgctcggta cggccagaat ggccatctgg attcagcagg gggtgattgc 7200 ggttacatca tgtacggaaa aataattcta atccttgatt taaatttgaa cttgactatt 7260 tatttattct ttatttcatt ttgtaaatca ttttatgtat ctcctggcaa gcaattttat 7320 ccaccttgca ccaacacctt cgggttccat aatcaaacca ccttaacttc acaccatgct 7380 gtaactcaca ccgcccagca tctccaatgt gaaagaagct aaaatttaat aaacaatcat 7440 acgaagcagt gacaaaatac cagatggtat taatgcttcg ataaaattaa ttggaaagta 7500 taaaatggta gaaaataata aattataatt aatttaagta agataaaaaa taattaaaaa 7560 ctaaaatgtt aaaattttaa aaaaattatt ttaaataata tttaaaaaca ttaaaaatca 7620 ttttaaaaaa tttatttata gaacaattaa ataaatattt cagctaataa aaaacaaaag 7680 cttacctagc cttagaagac aacttgtcca acaattagat gatacccatt gcccttacgt 7740 tttctttaac atcaattatt gtttttgtca acaagctatc ttttagtttt attttattgg 7800 taaaaaatat gtcgccttca agttgcatca tttaacacat ctcgtcatta gaaaaataaa 7860 actcttccct aaacgattag tagaaaaaat cattcgataa taaataagaa agaaaaatta 7920 gaaaaaaata acttcatttt aaaaaaatca ttaaggctat attttttaaa tgactaattt 7980 tatatagact gtaactaaaa gtatacaatt tattatgcta tgtatcttaa agaattactt 8040 ataaaaatct acggaagaat atcttacaaa gtgaaaaaca aatgagaaag aatttagtgg 8100 gatgattatg attttatttg aaaattgaaa aaataattat taaagacttt agtggagtaa 8160 gaaagctttc ctattagtct tttcttatcc ataaaaaaaa aaaaaaaaat ctagcgtgac 8220 agcttttcca tagattttaa taatgtaaaa tactggtagc agccgaccgt tcaggtaatg 8280 gacactgtgg tcctaacttg caacgggtgc gggcccaatt taataacgcc gtggtaacgg 8340 ataaagccaa gcgtgaagcg gtgaaggtac atctctgact ccgtcaagat tacgaaaccg 8400 tcaactacga aggactcccc gaaatatcat ctgtgtcata aacaccaagt cacaccatac 8460 atgggcacgc gtcacaatat gattggagaa cggttccacc gcatatgcta taaaatgccc 8520 ccacacccct cgaccctaat cgcacttcaa ttgcaatcaa attagttcat tctctttgcg 8580 cagttcccta cctctccttt caaggttcgt agatttcttc cgtttttttt tcttcttctt 8640 tattgtttgt tctacatcag catgatgttg atttgattgt gttttctatc gtttcatcga 8700 ttataaattt tcataatcag aagattcagc ttttattaat gcaagaacgt ccttaattga 8760 tgattttata accgtaaatt aggtctaatt agagtttttt tcataaagat tttcagatcc 8820 gtttacaaca agccttaatt gttgattctg tagtcgtaga ttaaggtttt tttcatgaac 8880 tacttcagat ccgttaaaca acagccttat ttgttgatac ttcagtcgtt tttcaagaaa 8940 ttgttcagat ccgttgataa aagccttatt cgttgattct gtatggtatt tcaagagata 9000 ttgctcaggt cctttagcaa ctaccttatt tgttgattct gtggccatag attaggattt 9060 tttttcacga aattgcttct tgaaattacg tgatggattt tgattctgat ttatcttgtg 9120 attgttgact ctacagccat ggctgcaact actcttacat ctgctttacc tggtgctttc 9180 agttcatccc agagaccaag tgctcctttc aacttacaaa gatcacctag agtgcttaga 9240 aggttcaaca gaaagacagg acgtcaacct cgtggacttg ttcgtgctgc aaaggctcaa 9300 gcggcgcaaa acctgccaga gaaattcgat gaaacaagac tctgggaggc cctcagggga 9360 tttggggtta gtccaagtag ggttacttac gctcccgtcg gattcggaga ttaccactgg 9420 accgtcacag atgaagacgg gagaccttgg ttcgcaactg tcagtgacct cgaacacaaa 9480 gagcattgcg ggcagggagc acaagcagcc cttaaaggac tcagacaagc tatggacact 9540 gccctcgcac ttagagatag ggacggactc agattcgtgg ttgctccagt ggcagcaaca 9600 gatgggggtg gccctgtcct tccccttgac gctaggtatg cactcacagt cttccctcac 9660 gtcccaggaa gaacaggaga gttcggacaa agactcacag aggcagagag ggacagactg 9720 cttgctcttc tggcagaact ccacggaaga acacctcctg aaacaacccc accagccgat 9780 atggaaccac caggacttcc tggtgttaga gcagcactcg ccgaaagtga aggaccttgg 9840 agtggaggac catttgcaga acccgctaga cttcttctca gagaacacga ggccacactg 9900 cacgcaagac tcgctgaatt cgaagccctc gtcgcaagag ttaagggtcg tggagcacct 9960 cttgtcgtta cccacggaga accacaccca ggcaacttga tcctcggaga ggaaggctat 10020 ctcctcgtgg actgggatac agttggactg gctccagctg aaagggattt gagtctcatc 10080 tcagacgacc cagctgacct cgcaagatac gcagagctta caggaagaac tccagaccca 10140 gacgcactcg cactctatag actgcgttgg agtctccttg atgtggctga gttcgtcgag 10200 tggttcaggg ctgaacacca aagaacccaa gacaccgaga gtgcatggaa ggggttcact 10260 gacacacttg ggcaattggc tgctggtgga tcagctgcat agcaaactta tctctgttat 10320 gaatcagaag aagttcatgt ctcgtttcat ttaaaacttt ggtggtttgt gttttggggc 10380 cttgtaaagc ccctgatgaa taattgttca actatgtttc cgttcctgtg ttataccttt 10440 ctttctaatg agtaatgaca tcaaacttct tctgtattga aattatgtcc ttgtgagtct 10500 ctttatcatc gtttcgtctt tacattatat gtgctacttt tgtctaatga gcctgaaaag 10560 tggctccaat ggtacgcact ggaagatttg ttggcttctg gtagatatag cgacagtgtt 10620 gagcttgtaa tatcatgtct cttattgcta aattagttcc tttcttaaca gaaaccttca 10680 aagtttttgt ttttgttttc atttacctaa tgtacacata cgctggccat gactaacaac 10740 atgtccaggc ttagagcata tttttttcta gcttaaattg ttaacttgtc attcagtaaa 10800 atccgagaat tgtgaagctc taattgaagc taattcgttt tataaagtca gttaaaaagt 10860 atactaaatt atccaacttt tcttcaaaat ctcaaaattc tatgacaaaa cgatagtctt 10920 tgtttatgtc agtaccacaa agaggtggaa aaaaacacca aaaaaacaat aagcaaacta 10980 tacactgaga agaaaaataa aagagagctc aatagatgtt ttatactaac ggtagattag 11040 atcaaagatc caagctttac tctacataga gcagaaccca gaatcccttc atatctcttt 11100 tattctagca ccgataatct actgaaaaga agacacttag agctctgtct ctttgtcaaa 11160 gaagtcccag ccgtcatcca gaagctcctt acgttcatta acagaggccg caattggatc 11220 gggtttactt attttgtggg tatctatact tttattagat ttttaatcag gctcctgatt 11280 tctttttatt tcgattgaat tcctgaactt gtattattca gtagatcgaa taaattataa 11340 aaagataaaa tcataaaata atattttatc ctatcaatca tattaaagca atgaatatgt 11400 aaaattaatc ttatctttat tttaaaaaat catataggtt tagtattttt ttaaaaataa 11460 agataggatt agttttacta ttcactgctt attactttta aaaaaatcat aaaggtttag 11520 tattttttta aaataaatat aggaatagtt ttactattca ctgctttaat agaaaaatag 11580 tttaaaattt aagatagttt taatcccagc atttgccacg tttgaacgtg agccgaaacg 11640 atgtcgttac attatcttaa cctagctgaa acgatgtcgt cataatatcg ccaaatgcca 11700 actggactac gtcgaaccca caaatcccac aaagcgcgtg aaatcaaatc gctcaaacca 11760 caaaaaagaa caacgcgttt gttacacgct caatcccacg cgagtagagc acagtaacct 11820 tcaaataagc gaatggggca taatcagaaa tccgaaataa acctaggggc attatcggaa 11880 atgaaaagta gctcactcaa tataaaaatc taggaaccct agttttcgtt atcactctgt 11940 gctccctcgc tctatttctc agtctctgtg tttgcggctg aggattccga acgagtgacc 12000 ttcttcgttt ctcgcaaagg taacagcctc tgctcttgtc tcttcgattc gatctatgcc 12060 tgtctcttat ttacgatgat gtttcttcgg ttatgttttt ttatttatgc tttatgctgt 12120 tgatgttcgg ttgtttgttt cgctttgttt ttgtggttca gttttttagg attcttttgg 12180 tttttgaatc gattaatcgg aagagatttt cgagttattt ggtgtgttgg aggtgaatct 12240 tttttttgag gtcatagatc tgttgtattt gtgttataaa catgcgactt tgtatgattt 12300 tttacgaggt tatgatgttc tggttgtttt attatgaatc tgttgagaca gaaccatgat 12360 ttttgttgat gttcgtttac actattaaag gtttgtttta acaggattaa aagtttttta 12420 agcatgttga aggagtcttg tagatatgta accgtcgata gtttttttgt gggtttgttc 12480 acatgttatc aagcttaatc ttttactatg tatgcgacca tatctggatc cagcaaaggc 12540 gattttttaa ttccttgtga aacttttgta atatgaagtt gaaattttgt tattggtaaa 12600 ctataaatgt gtgaagttgg agtatacctt taccttctta tttggctttg tgatagttta 12660 atttatatgt attttgagtt ctgacttgta tttctttgaa ttgattctag tttaagtaat 12720 catggcctcc tccgagaacg tcatcaccga gttcatgcgc ttcaaggtgc gcatggaggg 12780 caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 12840 ccacaacacc gtgaagctga aggtgacgaa gggcggcccc ctgcccttcg cctgggacat 12900 cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc 12960 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 13020 ggacggcggc gtggcgaccg tgacccagga ctcctccctg caggacggct gcttcatcta 13080 caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtgatgc agaagaagac 13140 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga 13200 gacccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat 13260 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggacg ccaagctgga 13320 catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgca ccgagggccg 13380 ccaccacctg ttcctgtagt tttgtgatct gatgataagt ggttggttcg tgtctcatgc 13440 acttgggagg tgatctattt cacctggtgt agtttgtgtt tccgtcagtt ggaaaaactt 13500 atccctatcg atttcgtttt cattttctgc ttttctttta tgtaccttcg tttgggcttg 13560 taacgggcct ttgtatttca actctcaata ataatccaag tgcatgttaa acaatttgtc 13620 atctgtttcg gctttgatat actactggtg aagatgggcc gtactactgc atcacaacga 13680 aaaataataa taagatgaaa aacttgaagt ggaaaaaaaa aaaaacttga atgttcacta 13740 ctactcattg accataatgt ttaacataca tagctcaata gtatttttgt gaatatggca 13800 acacaaacag tccaaaacaa ttgtctctta ctataccaaa ccaagggcgc cgcttgtttg 13860 ccactctttg tgtgcaatag tgtgattacc acatctccac attcaatata ttccctgaat 13920 tatctgacga ttttgatggc tcactgtttt cccaagtctt gaattgtctt ctgtgcgcca 13980 gtcaaatgca tatgtgttga gtttatcttt taaatatcaa gcttttgttt ttaacttttg 14040 tttgtaacca aaaactcaca gtaggagttt gatcacataa ttttatgttt gcctttgcaa 14100 tttctagtga gtctttgatt aaaagcttga aaagaaaatg cagccaagct taccaagtaa 14160 gttatgtgta ttaaccagag gaagagagaa tcttgcaaaa tttcaacaaa cacaaaaaga 14220 agtattacta cgattggtgg agaaagaaaa cgattccaaa tcttgaactg ttgttgtaaa 14280 agcatagcag aaagtgggag acaaccgaaa tagaaatgac tataacttaa tttaatgtta 14340 tcattataat ttcttctagc aaatatttag aaagtaaata tcacatcaac ctttaatgta 14400 attaagcttt ctctttttga ttcatgtgag atgaaaagaa aaaaaagaag agaaaagtgt 14460 agaaaacaca tcatttctaa gctgaagctg aggtacaaat taattaataa gtgactaggg 14520 tcacgtgacc ctagtcactt atgattccga tgacttcgta ggttcctagc tcaagccgct 14580 cgtgtccaag cgtcacttac gattagctaa tgattacggc atctaggacc gactagttct 14640 tatgtgcttc tagtctccaa atgtggttga tagttatttt gctctaagat caacagtaat 14700 gaagtataaa tcatcgttgt ggtgtgctac tcggttaatt gagcattaac acacacaaac 14760 atgacgagga tggtataatc tccaaaaatg tgtactttgt taggtgggac cctatagcct 14820 tgattaatgt gctatgttag gcatgcctgg aaacgtgtga cgcatatgtt ttgtgaacct 14880 gttgatatta tatgtgcttt tatattacca tattttatta aaatactaat atttattact 14940 agtaagatat aacattctat ctagcttaaa aactaaccat aaatattcca taataactag 15000 atttaccaaa ctaatatact aaatatacat aataaataca aaattaacaa gacaataatc 15060 aatatttatg agcttaatat atttagacat tatggttggt cgacgataat catgctaact 15120 tttcgtaatt gcttgattga aatatgctta gaataatgcc tctttgttct acatggcaaa 15180 tagggaccat tatggtgtaa caccctggga accacaaaca ccccgaaatg ctactaaact 15240 acacaactaa ccttcatata taaaatttcg acagcatctc ctttgaaaat ttgcatagac 15300 gtggaagcaa cagagtataa acagatctag ctagctaagt ttaaacggcc ggccaaacgt 15360 gcaagcgcta ctagacaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc 15420 taagcgtcaa tttatttaca attgaatata tcctgcca 15458 <210> 158 <211> 15376 <212> DNA <213> Artificial Sequence <220> <223> RV026524 T-DNA <400> 158 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaacctcaac aacaacagca gcagcagcaa aaccaacaac aaccaaacga tgacaatagc 1860 agcggtgcca aaggaaactt tctttgcagg caaagcagta ctaggtggac tcccacaact 1920 gaccaaataa gaatattgaa ggacctttac tacaacagtg gagttaggtc cccaagtgca 1980 gagcagattc agaggatatc tgctaggctt agacagtacg gtaagattga aggcaagaat 2040 gtcttttatt ggtttcagaa ccataaagct cgtgagaggc agaagaaaag gttcaccact 2100 gatgtcccca tgcaacaaag aactgtttcg aatgcttcta gttggaagcc tgaagactat 2160 tccttccaca acaaataccc caacattgct cctgggtttt cttctgcatc tccatcctca 2220 gctgctgggc ttcctgttgg acagatggga aactatgggt atggatctgt aactatggag 2280 aagagtttta gggactgctc aatatcagct actgccaacg gtggtgttgg tggatccatg 2340 agccccaact gtgggtgggt tggcattgat ccctactgtt catcctactc tctctttgac 2400 aagcaaaaat caactaatga aaccctagaa gatgaagaac aagatcaaga tcaagaagag 2460 gaagcaactc cagggattga aaccctccct ctcttcccta cccagagaga agacatcaat 2520 ggcttctgca acatgaagca ccaccccatc tgctactccg aaaactactg ctatggctct 2580 gacgatggaa acaacgcttc gcgtacttcc ctcgaactta gcctcaactc ttacagcaat 2640 gggcaggcac gggattccat ctaaggtacc tgacatctta tagtctgcaa cctctcgtgt 2700 ctgaattcct atctttatca agtgttattg cttccacgac tataggacag ctttcgtcga 2760 aaggttttgc tcatgtgatc tcgaaggatt catctagtct gatttttcgt gacttgtatc 2820 ggttttattg gattcatcca acatatatca ataaaaaatg agttgtgttt cctttcttcc 2880 tagttcagtt aaaattattt ccctcctgcg cttgtgctgt aattgtctgt gtacctgttg 2940 tttgtgactg tgttagttcc cttggatatg atttcgtatt tgatatgtac atggagatag 3000 cttagcttca ttattggagt atgaagttag tatgacatag tcactctcct ggaaaattga 3060 cactgcaaac catattttta ttctgaacca caaatcctag tcagtccgct ggcatatgcc 3120 gtccgtttgc tgaatccaga acgtgggttt ggagatgtac ggctgagatg cctctatgcg 3180 aaggggattt cgtggtgaaa cgagatggga gtagagcaac gcccgtggaa gatgcttcaa 3240 acttccacac ttttgagcaa cgatcggcag tagtaaggta gacgatttca agatcaaagc 3300 atatgaagat aaacaacatc aacaacaaaa tttgttgggg ttctatagag agaaacagag 3360 ctacatacat acactgtttt gtatctacca tctgagatga tgaaaagatg aaaaactaaa 3420 gaatgccccg gcgccaacgc caggacacgc cgcgcgcgcg tcacccgagc catctcttga 3480 cccagccggc gctgtatatt tacacacgtt gcagcatcga tcaccacctg ttcgatcgcg 3540 tcgccgtcac caagcttggc gcgccggtca cccggtccgg gcctagaagg ccacccgggg 3600 ctcggcgtta ggcttttagt ccgctaggtg gctagaccga ggttaggacg ctagttcact 3660 aggggcctag tggtgtttta aggatgggca agtttgtaca aaaaagcagg ctggcgccgg 3720 aaccaattca gtcgagtgcg gccgccagtg tgatggatat ctcgacccgg gtgattgcgg 3780 ttacatcatg tacggaaaaa taattctaat ccttgattta aatttgaact tgactattta 3840 tttattcttt atttcatttt gtaaatcatt ttatgtatct cctggcaagc aattttatcc 3900 accttgcacc aacaccttcg ggttccataa tcaaaccacc ttaacttcac accatgctgt 3960 aactcacacc gcccagcatc tccaatgtga aagaagctaa aatttaataa acaatcatac 4020 gaagcagtga caaaatacca gatggtatta atgcttcgat aaaattaatt ggaaagtata 4080 aaatggtaga aaataataaa ttataattaa tttaagtaag ataaaaaata attaaaaact 4140 aaaatgttaa aattttaaaa aaattatttt aaataatatt taaaaacatt aaaaatcatt 4200 ttaaaaaatt tatttataga acaattaaat aaatatttca gctaataaaa aacaaaagct 4260 tacctagcct tagaagacaa cttgtccaac aattagatga tacccattgc ccttacgttt 4320 tctttaacat caattattgt ttttgtcaac aagctatctt ttagttttat tttattggta 4380 aaaaatatgt cgccttcaag ttgcatcatt taacacatct cgtcattaga aaaataaaac 4440 tcttccctaa acgattagta gaaaaaatca ttcgataata aataagaaag aaaaattaga 4500 aaaaaataac ttcattttaa aaaaatcatt aaggctatat tttttaaatg actaatttta 4560 tatagactgt aactaaaagt atacaattta ttatgctatg tatcttaaag aattacttat 4620 aaaaatctac ggaagaatat cttacaaagt gaaaaacaaa tgagaaagaa tttagtggga 4680 tgattatgat tttatttgaa aattgaaaaa ataattatta aagactttag tggagtaaga 4740 aagctttcct attagtcttt tcttatccat aaaaaaaaaa aaaaaaatct agcgtgacag 4800 cttttccata gattttaata atgtaaaata ctggtagcag ccgaccgttc aggtaatgga 4860 cactgtggtc ctaacttgca acgggtgcgg gcccaattta ataacgccgt ggtaacggat 4920 aaagccaagc gtgaagcggt gaaggtacat ctctgactcc gtcaagatta cgaaaccgtc 4980 aactacgaag gactccccga aatatcatct gtgtcataaa caccaagtca caccatacat 5040 gggcacgcgt cacaatatga ttggagaacg gttccaccgc atatgctata aaatgccccc 5100 acacccctcg accctaatcg cacttcaatt gcaatcaaat tagttcattc tctttgcgca 5160 gttccctacc tctcctttca aggttcgtag atttcttccg tttttttttc ttcttcttta 5220 ttgtttgttc tacatcagca tgatgttgat ttgattgtgt tttctatcgt ttcatcgatt 5280 ataaattttc ataatcagaa gattcagctt ttattaatgc aagaacgtcc ttaattgatg 5340 attttataac cgtaaattag gtctaattag agtttttttc ataaagattt tcagatccgt 5400 ttacaacaag ccttaattgt tgattctgta gtcgtagatt aaggtttttt tcatgaacta 5460 cttcagatcc gttaaacaac agccttattt gttgatactt cagtcgtttt tcaagaaatt 5520 gttcagatcc gttgataaaa gccttattcg ttgattctgt atggtatttc aagagatatt 5580 gctcaggtcc tttagcaact accttatttg ttgattctgt ggccatagat taggattttt 5640 tttcacgaaa ttgcttcttg aaattacgtg atggattttg attctgattt atcttgtgat 5700 tgttgactct acagccatgg cccacagcaa gcacggcctg aaggaggaga tgaccatgaa 5760 gtaccacatg gagggctgcg tgaacggcca caagttcgtg atcaccggcg agggcatcgg 5820 ctaccccttc aagggcaagc agaccatcaa cctgtgcgtg atcgagggcg gccccctgcc 5880 cttcagcgag gacatcctga gcgccggctt caagtacggc gaccggatct tcaccgagta 5940 cccccaggac atcgtggact acttcaagaa cagctgcccc gccggctaca cctggggccg 6000 gagcttcctg ttcgaggacg gcgccgtgtg catctgtaac gtggacatca ccgtgagcgt 6060 gaaggagaac tgcatctacc acaagagcat cttcaacggc gtgaacttcc ccgccgacgg 6120 ccccgtgatg aagaagatga ccaccaactg ggaggccagc tgcgagaaga tcatgcccgt 6180 gcctaagcag ggcatcctga agggcgacgt gagcatgtac ctgctgctga aggacggcgg 6240 ccggtaccgg tgccagttcg acaccgtgta caaggccaag agcgtgccca gcaagatgcc 6300 cgagtggcac ttcatccagc acaagctgct gcgggaggac cggagcgacg ccaagaacca 6360 gaagtggcag ctgaccgagc acgccatcgc cttccccagc gccctggcct gagagctcga 6420 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg 6480 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca 6540 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca 6600 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg 6660 tgtcatctat gttactagat cgggaattct agtggccggc ccagctgata tctagaccca 6720 gctttcttgt acaaagtggc cgttaacgga tcggccagaa tggcccggac cgggttaccg 6780 aattcgagct cggtaccctg ggatccgata tcgatgggcc ctggccgaag cttggtcacc 6840 cggtccgggc ctagaaggcc agcttcggcc gccccgggca actttattat acaaagttga 6900 tggtacggat ccgtcgacgg cgcgccgcgg acctgaattc cagcacactg gcggccgtta 6960 gtctaaaaac ggcccctaac taacgcttag ttgcccgcta gattagtccc ccgttagtgc 7020 ctagttagac acacgacgaa ctaggggcta ctagtaacgg ccgccagtgt gctcggtacg 7080 gccagaatgg ccatctggat tcagcagggg gtgattgcgg ttacatcatg tacggaaaaa 7140 taattctaat ccttgattta aatttgaact tgactattta tttattcttt atttcatttt 7200 gtaaatcatt ttatgtatct cctggcaagc aattttatcc accttgcacc aacaccttcg 7260 ggttccataa tcaaaccacc ttaacttcac accatgctgt aactcacacc gcccagcatc 7320 tccaatgtga aagaagctaa aatttaataa acaatcatac gaagcagtga caaaatacca 7380 gatggtatta atgcttcgat aaaattaatt ggaaagtata aaatggtaga aaataataaa 7440 ttataattaa tttaagtaag ataaaaaata attaaaaact aaaatgttaa aattttaaaa 7500 aaattatttt aaataatatt taaaaacatt aaaaatcatt ttaaaaaatt tatttataga 7560 acaattaaat aaatatttca gctaataaaa aacaaaagct tacctagcct tagaagacaa 7620 cttgtccaac aattagatga tacccattgc ccttacgttt tctttaacat caattattgt 7680 ttttgtcaac aagctatctt ttagttttat tttattggta aaaaatatgt cgccttcaag 7740 ttgcatcatt taacacatct cgtcattaga aaaataaaac tcttccctaa acgattagta 7800 gaaaaaatca ttcgataata aataagaaag aaaaattaga aaaaaataac ttcattttaa 7860 aaaaatcatt aaggctatat tttttaaatg actaatttta tatagactgt aactaaaagt 7920 atacaattta ttatgctatg tatcttaaag aattacttat aaaaatctac ggaagaatat 7980 cttacaaagt gaaaaacaaa tgagaaagaa tttagtggga tgattatgat tttatttgaa 8040 aattgaaaaa ataattatta aagactttag tggagtaaga aagctttcct attagtcttt 8100 tcttatccat aaaaaaaaaa aaaaaaatct agcgtgacag cttttccata gattttaata 8160 atgtaaaata ctggtagcag ccgaccgttc aggtaatgga cactgtggtc ctaacttgca 8220 acgggtgcgg gcccaattta ataacgccgt ggtaacggat aaagccaagc gtgaagcggt 8280 gaaggtacat ctctgactcc gtcaagatta cgaaaccgtc aactacgaag gactccccga 8340 aatatcatct gtgtcataaa caccaagtca caccatacat gggcacgcgt cacaatatga 8400 ttggagaacg gttccaccgc atatgctata aaatgccccc acacccctcg accctaatcg 8460 cacttcaatt gcaatcaaat tagttcattc tctttgcgca gttccctacc tctcctttca 8520 aggttcgtag atttcttccg tttttttttc ttcttcttta ttgtttgttc tacatcagca 8580 tgatgttgat ttgattgtgt tttctatcgt ttcatcgatt ataaattttc ataatcagaa 8640 gattcagctt ttattaatgc aagaacgtcc ttaattgatg attttataac cgtaaattag 8700 gtctaattag agtttttttc ataaagattt tcagatccgt ttacaacaag ccttaattgt 8760 tgattctgta gtcgtagatt aaggtttttt tcatgaacta cttcagatcc gttaaacaac 8820 agccttattt gttgatactt cagtcgtttt tcaagaaatt gttcagatcc gttgataaaa 8880 gccttattcg ttgattctgt atggtatttc aagagatatt gctcaggtcc tttagcaact 8940 accttatttg ttgattctgt ggccatagat taggattttt tttcacgaaa ttgcttcttg 9000 aaattacgtg atggattttg attctgattt atcttgtgat tgttgactct acagccatgg 9060 ctgcaactac tcttacatct gctttacctg gtgctttcag ttcatcccag agaccaagtg 9120 ctcctttcaa cttacaaaga tcacctagag tgcttagaag gttcaacaga aagacaggac 9180 gtcaacctcg tggacttgtt cgtgctgcaa aggctcaagc ggcgcaaaac ctgccagaga 9240 aattcgatga aacaagactc tgggaggccc tcaggggatt tggggttagt ccaagtaggg 9300 ttacttacgc tcccgtcgga ttcggagatt accactggac cgtcacagat gaagacggga 9360 gaccttggtt cgcaactgtc agtgacctcg aacacaaaga gcattgcggg cagggagcac 9420 aagcagccct taaaggactc agacaagcta tggacactgc cctcgcactt agagataggg 9480 acggactcag attcgtggtt gctccagtgg cagcaacaga tgggggtggc cctgtccttc 9540 cccttgacgc taggtatgca ctcacagtct tccctcacgt cccaggaaga acaggagagt 9600 tcggacaaag actcacagag gcagagaggg acagactgct tgctcttctg gcagaactcc 9660 acggaagaac acctcctgaa acaaccccac cagccgatat ggaaccacca ggacttcctg 9720 gtgttagagc agcactcgcc gaaagtgaag gaccttggag tggaggacca tttgcagaac 9780 ccgctagact tcttctcaga gaacacgagg ccacactgca cgcaagactc gctgaattcg 9840 aagccctcgt cgcaagagtt aagggtcgtg gagcacctct tgtcgttacc cacggagaac 9900 cacacccagg caacttgatc ctcggagagg aaggctatct cctcgtggac tgggatacag 9960 ttggactggc tccagctgaa agggatttga gtctcatctc agacgaccca gctgacctcg 10020 caagatacgc agagcttaca ggaagaactc cagacccaga cgcactcgca ctctatagac 10080 tgcgttggag tctccttgat gtggctgagt tcgtcgagtg gttcagggct gaacaccaaa 10140 gaacccaaga caccgagagt gcatggaagg ggttcactga cacacttggg caattggctg 10200 ctggtggatc agctgcatag caaacttatc tctgttatga atcagaagaa gttcatgtct 10260 cgtttcattt aaaactttgg tggtttgtgt tttggggcct tgtaaagccc ctgatgaata 10320 attgttcaac tatgtttccg ttcctgtgtt atacctttct ttctaatgag taatgacatc 10380 aaacttcttc tgtattgaaa ttatgtcctt gtgagtctct ttatcatcgt ttcgtcttta 10440 cattatatgt gctacttttg tctaatgagc ctgaaaagtg gctccaatgg tacgcactgg 10500 aagatttgtt ggcttctggt agatatagcg acagtgttga gcttgtaata tcatgtctct 10560 tattgctaaa ttagttcctt tcttaacaga aaccttcaaa gtttttgttt ttgttttcat 10620 ttacctaatg tacacatacg ctggccatga ctaacaacat gtccaggctt agagcatatt 10680 tttttctagc ttaaattgtt aacttgtcat tcagtaaaat ccgagaattg tgaagctcta 10740 attgaagcta attcgtttta taaagtcagt taaaaagtat actaaattat ccaacttttc 10800 ttcaaaatct caaaattcta tgacaaaacg atagtctttg tttatgtcag taccacaaag 10860 aggtggaaaa aaacaccaaa aaaacaataa gcaaactata cactgagaag aaaaataaaa 10920 gagagctcaa tagatgtttt atactaacgg tagattagat caaagatcca agctttactc 10980 tacatagagc agaacccaga atcccttcat atctctttta ttctagcacc gataatctac 11040 tgaaaagaag acacttagag ctctgtctct ttgtcaaaga agtcccagcc gtcatccaga 11100 agctccttac gttcattaac agaggccgca attggatcgg gtttacttat tttgtgggta 11160 tctatacttt tattagattt ttaatcaggc tcctgatttc tttttatttc gattgaattc 11220 ctgaacttgt attattcagt agatcgaata aattataaaa agataaaatc ataaaataat 11280 attttatcct atcaatcata ttaaagcaat gaatatgtaa aattaatctt atctttattt 11340 taaaaaatca tataggttta gtattttttt aaaaataaag ataggattag ttttactatt 11400 cactgcttat tacttttaaa aaaatcataa aggtttagta tttttttaaa ataaatatag 11460 gaatagtttt actattcact gctttaatag aaaaatagtt taaaatttaa gatagtttta 11520 atcccagcat ttgccacgtt tgaacgtgag ccgaaacgat gtcgttacat tatcttaacc 11580 tagctgaaac gatgtcgtca taatatcgcc aaatgccaac tggactacgt cgaacccaca 11640 aatcccacaa agcgcgtgaa atcaaatcgc tcaaaccaca aaaaagaaca acgcgtttgt 11700 tacacgctca atcccacgcg agtagagcac agtaaccttc aaataagcga atggggcata 11760 atcagaaatc cgaaataaac ctaggggcat tatcggaaat gaaaagtagc tcactcaata 11820 taaaaatcta ggaaccctag ttttcgttat cactctgtgc tccctcgctc tatttctcag 11880 tctctgtgtt tgcggctgag gattccgaac gagtgacctt cttcgtttct cgcaaaggta 11940 acagcctctg ctcttgtctc ttcgattcga tctatgcctg tctcttattt acgatgatgt 12000 ttcttcggtt atgttttttt atttatgctt tatgctgttg atgttcggtt gtttgtttcg 12060 ctttgttttt gtggttcagt tttttaggat tcttttggtt tttgaatcga ttaatcggaa 12120 gagattttcg agttatttgg tgtgttggag gtgaatcttt tttttgaggt catagatctg 12180 ttgtatttgt gttataaaca tgcgactttg tatgattttt tacgaggtta tgatgttctg 12240 gttgttttat tatgaatctg ttgagacaga accatgattt ttgttgatgt tcgtttacac 12300 tattaaaggt ttgttttaac aggattaaaa gttttttaag catgttgaag gagtcttgta 12360 gatatgtaac cgtcgatagt ttttttgtgg gtttgttcac atgttatcaa gcttaatctt 12420 ttactatgta tgcgaccata tctggatcca gcaaaggcga ttttttaatt ccttgtgaaa 12480 cttttgtaat atgaagttga aattttgtta ttggtaaact ataaatgtgt gaagttggag 12540 tataccttta ccttcttatt tggctttgtg atagtttaat ttatatgtat tttgagttct 12600 gacttgtatt tctttgaatt gattctagtt taagtaatca tggcctcctc cgagaacgtc 12660 atcaccgagt tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg ccacgagttc 12720 gagatcgagg gcgagggcga gggccgcccc tacgagggcc acaacaccgt gaagctgaag 12780 gtgacgaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac 12840 ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc 12900 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg 12960 acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt catcggcgtg 13020 aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctccacc 13080 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga cccacaaggc cctgaagctg 13140 aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg 13200 cagctgcccg gctactacta cgtggacgcc aagctggaca tcacctccca caacgaggac 13260 tacaccatcg tggagcagta cgagcgcacc gagggccgcc accacctgtt cctgtagttt 13320 tgtgatctga tgataagtgg ttggttcgtg tctcatgcac ttgggaggtg atctatttca 13380 cctggtgtag tttgtgtttc cgtcagttgg aaaaacttat ccctatcgat ttcgttttca 13440 ttttctgctt ttcttttatg taccttcgtt tgggcttgta acgggccttt gtatttcaac 13500 tctcaataat aatccaagtg catgttaaac aatttgtcat ctgtttcggc tttgatatac 13560 tactggtgaa gatgggccgt actactgcat cacaacgaaa aataataata agatgaaaaa 13620 cttgaagtgg aaaaaaaaaa aaacttgaat gttcactact actcattgac cataatgttt 13680 aacatacata gctcaatagt atttttgtga atatggcaac acaaacagtc caaaacaatt 13740 gtctcttact ataccaaacc aagggcgccg cttgtttgcc actctttgtg tgcaatagtg 13800 tgattaccac atctccacat tcaatatatt ccctgaatta tctgacgatt ttgatggctc 13860 actgttttcc caagtcttga attgtcttct gtgcgccagt caaatgcata tgtgttgagt 13920 ttatctttta aatatcaagc ttttgttttt aacttttgtt tgtaaccaaa aactcacagt 13980 aggagtttga tcacataatt ttatgtttgc ctttgcaatt tctagtgagt ctttgattaa 14040 aagcttgaaa agaaaatgca gccaagctta ccaagtaagt tatgtgtatt aaccagagga 14100 agagagaatc ttgcaaaatt tcaacaaaca caaaaagaag tattactacg attggtggag 14160 aaagaaaacg attccaaatc ttgaactgtt gttgtaaaag catagcagaa agtgggagac 14220 aaccgaaata gaaatgacta taacttaatt taatgttatc attataattt cttctagcaa 14280 atatttagaa agtaaatatc acatcaacct ttaatgtaat taagctttct ctttttgatt 14340 catgtgagat gaaaagaaaa aaaagaagag aaaagtgtag aaaacacatc atttctaagc 14400 tgaagctgag gtacaaatta attaataagt gactagggtc acgtgaccct agtcacttat 14460 gattccgatg acttcgtagg ttcctagctc aagccgctcg tgtccaagcg tcacttacga 14520 ttagctaatg attacggcat ctaggaccga ctagttctta tgtgcttcta gtctccaaat 14580 gtggttgata gttattttgc tctaagatca acagtaatga agtataaatc atcgttgtgg 14640 tgtgctactc ggttaattga gcattaacac acacaaacat gacgaggatg gtataatctc 14700 caaaaatgtg tactttgtta ggtgggaccc tatagccttg attaatgtgc tatgttaggc 14760 atgcctggaa acgtgtgacg catatgtttt gtgaacctgt tgatattata tgtgctttta 14820 tattaccata ttttattaaa atactaatat ttattactag taagatataa cattctatct 14880 agcttaaaaa ctaaccataa atattccata ataactagat ttaccaaact aatatactaa 14940 atatacataa taaatacaaa attaacaaga caataatcaa tatttatgag cttaatatat 15000 ttagacatta tggttggtcg acgataatca tgctaacttt tcgtaattgc ttgattgaaa 15060 tatgcttaga ataatgcctc tttgttctac atggcaaata gggaccatta tggtgtaaca 15120 ccctgggaac cacaaacacc ccgaaatgct actaaactac acaactaacc ttcatatata 15180 aaatttcgac agcatctcct ttgaaaattt gcatagacgt ggaagcaaca gagtataaac 15240 agatctagct agctaagttt aaacggccgg ccaaacgtgc aagcgctact agacaattca 15300 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tatttacaat 15360 tgaatatatc ctgcca 15376 <210> 159 <211> 15431 <212> DNA <213> Artificial Sequence <220> <223> RV026523 T-DNA <400> 159 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccaagc ttggatctcc tgcaggatct ggccggccgg atgggcggat 1320 ctcgtacctc aactttgtat agaaaagttg ggccagaatg gcccggaccg ggttaccaag 1380 cggccgcgaa ttccagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcg 1440 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa 1500 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat 1560 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa 1620 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta 1680 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca 1740 tttcatttgg agaggaccca tggaagctca acagcaacag cataatacaa tgatgggtga 1800 tgaccttggc ggtggcaagt tggcagccac cagcttcaca agagcgcaaa ccaacagcag 1860 caacaaccac agcctagtta ataacaacgg cggcagaaga agcagcacga ggtggacacc 1920 aacaacagac cagataagaa tactcaaaga tctttactac aacaatggag tgaggtctcc 1980 cacggtggag cagattcaga ggatctctgc taggttgaga cagtacggta agatcgaggg 2040 caaaaatgtc ttctactggt ttcagaacca aaaggctcgt gaaagacaga agaggaggtt 2100 caacacaacc agcaccacca acaacaacac tgctcccgct cctctccctc catcttctac 2160 tgtttgtaaa ccttcttcgc tagactcatc cactccacac attcacactg cagcagcagc 2220 tgctgctgct gcctccaaat actcagacat caacaacaac caaaacctta tctacccagc 2280 tgggcacaat cagatgtcag gtttcggata tgctgcctcc gtaacaatgg agaagagctt 2340 cagagattgt tctatatcga cgggcgggtc atctttcggt tgggctaaag gaggaatgac 2400 agatcaccat ctgtctcgtc atccctaccc gcacacttca ccctatgcaa tctacaacca 2460 gcggaaatca ataaacagtg atgagtatca cgagctactg gcatcatcaa cttcggatca 2520 tcatgaacat ggggataatc ggggtccgga gattgaaacc ctgccactat ttcccatgca 2580 cggcgaggac atcaactacg gcttctgcca ccttaattca gccgagactg atggctggtt 2640 caatcgcgat gtgggtagtg gctctcgtgc gtccctcgag ctcagtctca actcttacgc 2700 aggaatggac cacacctagg gtacctgaca tcttatagtc tgcaacctct cgtgtctgaa 2760 ttcctatctt tatcaagtgt tattgcttcc acgactatag gacagctttc gtcgaaaggt 2820 tttgctcatg tgatctcgaa ggattcatct agtctgattt ttcgtgactt gtatcggttt 2880 tattggattc atccaacata tatcaataaa aaatgagttg tgtttccttt cttcctagtt 2940 cagttaaaat tatttccctc ctgcgcttgt gctgtaattg tctgtgtacc tgttgtttgt 3000 gactgtgtta gttcccttgg atatgatttc gtatttgata tgtacatgga gatagcttag 3060 cttcattatt ggagtatgaa gttagtatga catagtcact ctcctggaaa attgacactg 3120 caaaccatat ttttattctg aaccacaaat cctagtcagt ccgctggcat atgccgtccg 3180 tttgctgaat ccagaacgtg ggtttggaga tgtacggctg agatgcctct atgcgaaggg 3240 gatttcgtgg tgaaacgaga tgggagtaga gcaacgcccg tggaagatgc ttcaaacttc 3300 cacacttttg agcaacgatc ggcagtagta aggtagacga tttcaagatc aaagcatatg 3360 aagataaaca acatcaacaa caaaatttgt tggggttcta tagagagaaa cagagctaca 3420 tacatacact gttttgtatc taccatctga gatgatgaaa agatgaaaaa ctaaagaatg 3480 ccccggcgcc aacgccagga cacgccgcgc gcgcgtcacc cgagccatct cttgacccag 3540 ccggcgctgt atatttacac acgttgcagc atcgatcacc acctgttcga tcgcgtcgcc 3600 gtcaccaagc ttggcgcgcc ggtcacccgg tccgggccta gaaggccacc cggggctcgg 3660 cgttaggctt ttagtccgct aggtggctag accgaggtta ggacgctagt tcactagggg 3720 cctagtggtg ttttaaggat gggcaagttt gtacaaaaaa gcaggctggc gccggaacca 3780 attcagtcga gtgcggccgc cagtgtgatg gatatctcga cccgggtgat tgcggttaca 3840 tcatgtacgg aaaaataatt ctaatccttg atttaaattt gaacttgact atttatttat 3900 tctttatttc attttgtaaa tcattttatg tatctcctgg caagcaattt tatccacctt 3960 gcaccaacac cttcgggttc cataatcaaa ccaccttaac ttcacaccat gctgtaactc 4020 acaccgccca gcatctccaa tgtgaaagaa gctaaaattt aataaacaat catacgaagc 4080 agtgacaaaa taccagatgg tattaatgct tcgataaaat taattggaaa gtataaaatg 4140 gtagaaaata ataaattata attaatttaa gtaagataaa aaataattaa aaactaaaat 4200 gttaaaattt taaaaaaatt attttaaata atatttaaaa acattaaaaa tcattttaaa 4260 aaatttattt atagaacaat taaataaata tttcagctaa taaaaaacaa aagcttacct 4320 agccttagaa gacaacttgt ccaacaatta gatgataccc attgccctta cgttttcttt 4380 aacatcaatt attgtttttg tcaacaagct atcttttagt tttattttat tggtaaaaaa 4440 tatgtcgcct tcaagttgca tcatttaaca catctcgtca ttagaaaaat aaaactcttc 4500 cctaaacgat tagtagaaaa aatcattcga taataaataa gaaagaaaaa ttagaaaaaa 4560 ataacttcat tttaaaaaaa tcattaaggc tatatttttt aaatgactaa ttttatatag 4620 actgtaacta aaagtataca atttattatg ctatgtatct taaagaatta cttataaaaa 4680 tctacggaag aatatcttac aaagtgaaaa acaaatgaga aagaatttag tgggatgatt 4740 atgattttat ttgaaaattg aaaaaataat tattaaagac tttagtggag taagaaagct 4800 ttcctattag tcttttctta tccataaaaa aaaaaaaaaa aatctagcgt gacagctttt 4860 ccatagattt taataatgta aaatactggt agcagccgac cgttcaggta atggacactg 4920 tggtcctaac ttgcaacggg tgcgggccca atttaataac gccgtggtaa cggataaagc 4980 caagcgtgaa gcggtgaagg tacatctctg actccgtcaa gattacgaaa ccgtcaacta 5040 cgaaggactc cccgaaatat catctgtgtc ataaacacca agtcacacca tacatgggca 5100 cgcgtcacaa tatgattgga gaacggttcc accgcatatg ctataaaatg cccccacacc 5160 cctcgaccct aatcgcactt caattgcaat caaattagtt cattctcttt gcgcagttcc 5220 ctacctctcc tttcaaggtt cgtagatttc ttccgttttt ttttcttctt ctttattgtt 5280 tgttctacat cagcatgatg ttgatttgat tgtgttttct atcgtttcat cgattataaa 5340 ttttcataat cagaagattc agcttttatt aatgcaagaa cgtccttaat tgatgatttt 5400 ataaccgtaa attaggtcta attagagttt ttttcataaa gattttcaga tccgtttaca 5460 acaagcctta attgttgatt ctgtagtcgt agattaaggt ttttttcatg aactacttca 5520 gatccgttaa acaacagcct tatttgttga tacttcagtc gtttttcaag aaattgttca 5580 gatccgttga taaaagcctt attcgttgat tctgtatggt atttcaagag atattgctca 5640 ggtcctttag caactacctt atttgttgat tctgtggcca tagattagga ttttttttca 5700 cgaaattgct tcttgaaatt acgtgatgga ttttgattct gatttatctt gtgattgttg 5760 actctacagc catggcccac agcaagcacg gcctgaagga ggagatgacc atgaagtacc 5820 acatggaggg ctgcgtgaac ggccacaagt tcgtgatcac cggcgagggc atcggctacc 5880 ccttcaaggg caagcagacc atcaacctgt gcgtgatcga gggcggcccc ctgcccttca 5940 gcgaggacat cctgagcgcc ggcttcaagt acggcgaccg gatcttcacc gagtaccccc 6000 aggacatcgt ggactacttc aagaacagct gccccgccgg ctacacctgg ggccggagct 6060 tcctgttcga ggacggcgcc gtgtgcatct gtaacgtgga catcaccgtg agcgtgaagg 6120 agaactgcat ctaccacaag agcatcttca acggcgtgaa cttccccgcc gacggccccg 6180 tgatgaagaa gatgaccacc aactgggagg ccagctgcga gaagatcatg cccgtgccta 6240 agcagggcat cctgaagggc gacgtgagca tgtacctgct gctgaaggac ggcggccggt 6300 accggtgcca gttcgacacc gtgtacaagg ccaagagcgt gcccagcaag atgcccgagt 6360 ggcacttcat ccagcacaag ctgctgcggg aggaccggag cgacgccaag aaccagaagt 6420 ggcagctgac cgagcacgcc atcgccttcc ccagcgccct ggcctgagag ctcgaatttc 6480 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt 6540 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa 6600 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa 6660 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca 6720 tctatgttac tagatcggga attctagtgg ccggcccagc tgatatctag acccagcttt 6780 cttgtacaaa gtggccgtta acggatcggc cagaatggcc cggaccgggt taccgaattc 6840 gagctcggta ccctgggatc cgatatcgat gggccctggc cgaagcttgg tcacccggtc 6900 cgggcctaga aggccagctt cggccgcccc gggcaacttt attatacaaa gttgatggta 6960 cggatccgtc gacggcgcgc cgcggacctg aattccagca cactggcggc cgttagtcta 7020 aaaacggccc ctaactaacg cttagttgcc cgctagatta gtcccccgtt agtgcctagt 7080 tagacacacg acgaactagg ggctactagt aacggccgcc agtgtgctcg gtacggccag 7140 aatggccatc tggattcagc agggggtgat tgcggttaca tcatgtacgg aaaaataatt 7200 ctaatccttg atttaaattt gaacttgact atttatttat tctttatttc attttgtaaa 7260 tcattttatg tatctcctgg caagcaattt tatccacctt gcaccaacac cttcgggttc 7320 cataatcaaa ccaccttaac ttcacaccat gctgtaactc acaccgccca gcatctccaa 7380 tgtgaaagaa gctaaaattt aataaacaat catacgaagc agtgacaaaa taccagatgg 7440 tattaatgct tcgataaaat taattggaaa gtataaaatg gtagaaaata ataaattata 7500 attaatttaa gtaagataaa aaataattaa aaactaaaat gttaaaattt taaaaaaatt 7560 attttaaata atatttaaaa acattaaaaa tcattttaaa aaatttattt atagaacaat 7620 taaataaata tttcagctaa taaaaaacaa aagcttacct agccttagaa gacaacttgt 7680 ccaacaatta gatgataccc attgccctta cgttttcttt aacatcaatt attgtttttg 7740 tcaacaagct atcttttagt tttattttat tggtaaaaaa tatgtcgcct tcaagttgca 7800 tcatttaaca catctcgtca ttagaaaaat aaaactcttc cctaaacgat tagtagaaaa 7860 aatcattcga taataaataa gaaagaaaaa ttagaaaaaa ataacttcat tttaaaaaaa 7920 tcattaaggc tatatttttt aaatgactaa ttttatatag actgtaacta aaagtataca 7980 atttattatg ctatgtatct taaagaatta cttataaaaa tctacggaag aatatcttac 8040 aaagtgaaaa acaaatgaga aagaatttag tgggatgatt atgattttat ttgaaaattg 8100 aaaaaataat tattaaagac tttagtggag taagaaagct ttcctattag tcttttctta 8160 tccataaaaa aaaaaaaaaa aatctagcgt gacagctttt ccatagattt taataatgta 8220 aaatactggt agcagccgac cgttcaggta atggacactg tggtcctaac ttgcaacggg 8280 tgcgggccca atttaataac gccgtggtaa cggataaagc caagcgtgaa gcggtgaagg 8340 tacatctctg actccgtcaa gattacgaaa ccgtcaacta cgaaggactc cccgaaatat 8400 catctgtgtc ataaacacca agtcacacca tacatgggca cgcgtcacaa tatgattgga 8460 gaacggttcc accgcatatg ctataaaatg cccccacacc cctcgaccct aatcgcactt 8520 caattgcaat caaattagtt cattctcttt gcgcagttcc ctacctctcc tttcaaggtt 8580 cgtagatttc ttccgttttt ttttcttctt ctttattgtt tgttctacat cagcatgatg 8640 ttgatttgat tgtgttttct atcgtttcat cgattataaa ttttcataat cagaagattc 8700 agcttttatt aatgcaagaa cgtccttaat tgatgatttt ataaccgtaa attaggtcta 8760 attagagttt ttttcataaa gattttcaga tccgtttaca acaagcctta attgttgatt 8820 ctgtagtcgt agattaaggt ttttttcatg aactacttca gatccgttaa acaacagcct 8880 tatttgttga tacttcagtc gtttttcaag aaattgttca gatccgttga taaaagcctt 8940 attcgttgat tctgtatggt atttcaagag atattgctca ggtcctttag caactacctt 9000 atttgttgat tctgtggcca tagattagga ttttttttca cgaaattgct tcttgaaatt 9060 acgtgatgga ttttgattct gatttatctt gtgattgttg actctacagc catggctgca 9120 actactctta catctgcttt acctggtgct ttcagttcat cccagagacc aagtgctcct 9180 ttcaacttac aaagatcacc tagagtgctt agaaggttca acagaaagac aggacgtcaa 9240 cctcgtggac ttgttcgtgc tgcaaaggct caagcggcgc aaaacctgcc agagaaattc 9300 gatgaaacaa gactctggga ggccctcagg ggatttgggg ttagtccaag tagggttact 9360 tacgctcccg tcggattcgg agattaccac tggaccgtca cagatgaaga cgggagacct 9420 tggttcgcaa ctgtcagtga cctcgaacac aaagagcatt gcgggcaggg agcacaagca 9480 gcccttaaag gactcagaca agctatggac actgccctcg cacttagaga tagggacgga 9540 ctcagattcg tggttgctcc agtggcagca acagatgggg gtggccctgt ccttcccctt 9600 gacgctaggt atgcactcac agtcttccct cacgtcccag gaagaacagg agagttcgga 9660 caaagactca cagaggcaga gagggacaga ctgcttgctc ttctggcaga actccacgga 9720 agaacacctc ctgaaacaac cccaccagcc gatatggaac caccaggact tcctggtgtt 9780 agagcagcac tcgccgaaag tgaaggacct tggagtggag gaccatttgc agaacccgct 9840 agacttcttc tcagagaaca cgaggccaca ctgcacgcaa gactcgctga attcgaagcc 9900 ctcgtcgcaa gagttaaggg tcgtggagca cctcttgtcg ttacccacgg agaaccacac 9960 ccaggcaact tgatcctcgg agaggaaggc tatctcctcg tggactggga tacagttgga 10020 ctggctccag ctgaaaggga tttgagtctc atctcagacg acccagctga cctcgcaaga 10080 tacgcagagc ttacaggaag aactccagac ccagacgcac tcgcactcta tagactgcgt 10140 tggagtctcc ttgatgtggc tgagttcgtc gagtggttca gggctgaaca ccaaagaacc 10200 caagacaccg agagtgcatg gaaggggttc actgacacac ttgggcaatt ggctgctggt 10260 ggatcagctg catagcaaac ttatctctgt tatgaatcag aagaagttca tgtctcgttt 10320 catttaaaac tttggtggtt tgtgttttgg ggccttgtaa agcccctgat gaataattgt 10380 tcaactatgt ttccgttcct gtgttatacc tttctttcta atgagtaatg acatcaaact 10440 tcttctgtat tgaaattatg tccttgtgag tctctttatc atcgtttcgt ctttacatta 10500 tatgtgctac ttttgtctaa tgagcctgaa aagtggctcc aatggtacgc actggaagat 10560 ttgttggctt ctggtagata tagcgacagt gttgagcttg taatatcatg tctcttattg 10620 ctaaattagt tcctttctta acagaaacct tcaaagtttt tgtttttgtt ttcatttacc 10680 taatgtacac atacgctggc catgactaac aacatgtcca ggcttagagc atattttttt 10740 ctagcttaaa ttgttaactt gtcattcagt aaaatccgag aattgtgaag ctctaattga 10800 agctaattcg ttttataaag tcagttaaaa agtatactaa attatccaac ttttcttcaa 10860 aatctcaaaa ttctatgaca aaacgatagt ctttgtttat gtcagtacca caaagaggtg 10920 gaaaaaaaca ccaaaaaaac aataagcaaa ctatacactg agaagaaaaa taaaagagag 10980 ctcaatagat gttttatact aacggtagat tagatcaaag atccaagctt tactctacat 11040 agagcagaac ccagaatccc ttcatatctc ttttattcta gcaccgataa tctactgaaa 11100 agaagacact tagagctctg tctctttgtc aaagaagtcc cagccgtcat ccagaagctc 11160 cttacgttca ttaacagagg ccgcaattgg atcgggttta cttattttgt gggtatctat 11220 acttttatta gatttttaat caggctcctg atttcttttt atttcgattg aattcctgaa 11280 cttgtattat tcagtagatc gaataaatta taaaaagata aaatcataaa ataatatttt 11340 atcctatcaa tcatattaaa gcaatgaata tgtaaaatta atcttatctt tattttaaaa 11400 aatcatatag gtttagtatt tttttaaaaa taaagatagg attagtttta ctattcactg 11460 cttattactt ttaaaaaaat cataaaggtt tagtattttt ttaaaataaa tataggaata 11520 gttttactat tcactgcttt aatagaaaaa tagtttaaaa tttaagatag ttttaatccc 11580 agcatttgcc acgtttgaac gtgagccgaa acgatgtcgt tacattatct taacctagct 11640 gaaacgatgt cgtcataata tcgccaaatg ccaactggac tacgtcgaac ccacaaatcc 11700 cacaaagcgc gtgaaatcaa atcgctcaaa ccacaaaaaa gaacaacgcg tttgttacac 11760 gctcaatccc acgcgagtag agcacagtaa ccttcaaata agcgaatggg gcataatcag 11820 aaatccgaaa taaacctagg ggcattatcg gaaatgaaaa gtagctcact caatataaaa 11880 atctaggaac cctagttttc gttatcactc tgtgctccct cgctctattt ctcagtctct 11940 gtgtttgcgg ctgaggattc cgaacgagtg accttcttcg tttctcgcaa aggtaacagc 12000 ctctgctctt gtctcttcga ttcgatctat gcctgtctct tatttacgat gatgtttctt 12060 cggttatgtt tttttattta tgctttatgc tgttgatgtt cggttgtttg tttcgctttg 12120 tttttgtggt tcagtttttt aggattcttt tggtttttga atcgattaat cggaagagat 12180 tttcgagtta tttggtgtgt tggaggtgaa tctttttttt gaggtcatag atctgttgta 12240 tttgtgttat aaacatgcga ctttgtatga ttttttacga ggttatgatg ttctggttgt 12300 tttattatga atctgttgag acagaaccat gatttttgtt gatgttcgtt tacactatta 12360 aaggtttgtt ttaacaggat taaaagtttt ttaagcatgt tgaaggagtc ttgtagatat 12420 gtaaccgtcg atagtttttt tgtgggtttg ttcacatgtt atcaagctta atcttttact 12480 atgtatgcga ccatatctgg atccagcaaa ggcgattttt taattccttg tgaaactttt 12540 gtaatatgaa gttgaaattt tgttattggt aaactataaa tgtgtgaagt tggagtatac 12600 ctttaccttc ttatttggct ttgtgatagt ttaatttata tgtattttga gttctgactt 12660 gtatttcttt gaattgattc tagtttaagt aatcatggcc tcctccgaga acgtcatcac 12720 cgagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat 12780 cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac 12840 gaagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc 12900 caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga 12960 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca 13020 ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt 13080 cccctccgac ggccccgtga tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg 13140 cctgtacccc cgcgacggcg tgctgaaggg cgagacccac aaggccctga agctgaagga 13200 cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct 13260 gcccggctac tactacgtgg acgccaagct ggacatcacc tcccacaacg aggactacac 13320 catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt agttttgtga 13380 tctgatgata agtggttggt tcgtgtctca tgcacttggg aggtgatcta tttcacctgg 13440 tgtagtttgt gtttccgtca gttggaaaaa cttatcccta tcgatttcgt tttcattttc 13500 tgcttttctt ttatgtacct tcgtttgggc ttgtaacggg cctttgtatt tcaactctca 13560 ataataatcc aagtgcatgt taaacaattt gtcatctgtt tcggctttga tatactactg 13620 gtgaagatgg gccgtactac tgcatcacaa cgaaaaataa taataagatg aaaaacttga 13680 agtggaaaaa aaaaaaaact tgaatgttca ctactactca ttgaccataa tgtttaacat 13740 acatagctca atagtatttt tgtgaatatg gcaacacaaa cagtccaaaa caattgtctc 13800 ttactatacc aaaccaaggg cgccgcttgt ttgccactct ttgtgtgcaa tagtgtgatt 13860 accacatctc cacattcaat atattccctg aattatctga cgattttgat ggctcactgt 13920 tttcccaagt cttgaattgt cttctgtgcg ccagtcaaat gcatatgtgt tgagtttatc 13980 ttttaaatat caagcttttg tttttaactt ttgtttgtaa ccaaaaactc acagtaggag 14040 tttgatcaca taattttatg tttgcctttg caatttctag tgagtctttg attaaaagct 14100 tgaaaagaaa atgcagccaa gcttaccaag taagttatgt gtattaacca gaggaagaga 14160 gaatcttgca aaatttcaac aaacacaaaa agaagtatta ctacgattgg tggagaaaga 14220 aaacgattcc aaatcttgaa ctgttgttgt aaaagcatag cagaaagtgg gagacaaccg 14280 aaatagaaat gactataact taatttaatg ttatcattat aatttcttct agcaaatatt 14340 tagaaagtaa atatcacatc aacctttaat gtaattaagc tttctctttt tgattcatgt 14400 gagatgaaaa gaaaaaaaag aagagaaaag tgtagaaaac acatcatttc taagctgaag 14460 ctgaggtaca aattaattaa taagtgacta gggtcacgtg accctagtca cttatgattc 14520 cgatgacttc gtaggttcct agctcaagcc gctcgtgtcc aagcgtcact tacgattagc 14580 taatgattac ggcatctagg accgactagt tcttatgtgc ttctagtctc caaatgtggt 14640 tgatagttat tttgctctaa gatcaacagt aatgaagtat aaatcatcgt tgtggtgtgc 14700 tactcggtta attgagcatt aacacacaca aacatgacga ggatggtata atctccaaaa 14760 atgtgtactt tgttaggtgg gaccctatag ccttgattaa tgtgctatgt taggcatgcc 14820 tggaaacgtg tgacgcatat gttttgtgaa cctgttgata ttatatgtgc ttttatatta 14880 ccatatttta ttaaaatact aatatttatt actagtaaga tataacattc tatctagctt 14940 aaaaactaac cataaatatt ccataataac tagatttacc aaactaatat actaaatata 15000 cataataaat acaaaattaa caagacaata atcaatattt atgagcttaa tatatttaga 15060 cattatggtt ggtcgacgat aatcatgcta acttttcgta attgcttgat tgaaatatgc 15120 ttagaataat gcctctttgt tctacatggc aaatagggac cattatggtg taacaccctg 15180 ggaaccacaa acaccccgaa atgctactaa actacacaac taaccttcat atataaaatt 15240 tcgacagcat ctcctttgaa aatttgcata gacgtggaag caacagagta taaacagatc 15300 tagctagcta agtttaaacg gccggccaaa cgtgcaagcg ctactagaca attcagtaca 15360 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttattt acaattgaat 15420 atatcctgcc a 15431 <210> 160 <211> 15349 <212> DNA <213> Artificial Sequence <220> <223> RV026530 T-DNA <400> 160 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaaccccagc aacagcaaca gcaaaaccaa caagctccaa atgaggattg tggtggtggc 1860 agtggcaaag gaagctttct ttgcaggcaa agtagtacac ggtggactcc taccactgac 1920 cagattagaa tattgaagga cctttactac aacaatggag ttaggtcccc gagtgctgat 1980 cagattcaaa ggatctctgc tagacttaga cagtacggaa aaatcgaagg caagaatgtc 2040 ttttattggt ttcagaacca caaggctcgt gagaggcaga agaaaaggtt caccaccacg 2100 actaatcatc atgtccccat gcaaagccct accactgctg caaataacaa gtatcccaac 2160 ataactcctg gttattctcc tacatctctt tcttcaactg gtgctcttac tgttgggcag 2220 ataggaaact atggctatgg gtctataact atggaaaaga gttttaggga ttgctcaata 2280 tcagcatgtg gtagtagtac aagtgtaggc gaatccctga gtcacaactt tggatgggtt 2340 gggttcgatc ctacttattc ttcgtcctac acattctttg agcacaaaaa attcatggaa 2400 gagagacaaa aagatgaaga tgatgcagaa gaagaagctg ctgctccaca aattgagacc 2460 ctccctctct ttcccatgca cagtgaagac gctaatggtt tttgcagtaa tattaggccc 2520 agactagaca gctgctactc tgggtggtac aggtctgaag atggctacac tggttctcgt 2580 gcctcacttg agctcagcct caacccatat gcaggcagac cgcaagattc tatctgaggt 2640 acctgacatc ttatagtctg caacctctcg tgtctgaatt cctatcttta tcaagtgtta 2700 ttgcttccac gactatagga cagctttcgt cgaaaggttt tgctcatgtg atctcgaagg 2760 attcatctag tctgattttt cgtgacttgt atcggtttta ttggattcat ccaacatata 2820 tcaataaaaa atgagttgtg tttcctttct tcctagttca gttaaaatta tttccctcct 2880 gcgcttgtgc tgtaattgtc tgtgtacctg ttgtttgtga ctgtgttagt tcccttggat 2940 atgatttcgt atttgatatg tacatggaga tagcttagct tcattattgg agtatgaagt 3000 tagtatgaca tagtcactct cctggaaaat tgacactgca aaccatattt ttattctgaa 3060 ccacaaatcc tagtcagtcc gctggcatat gccgtccgtt tgctgaatcc agaacgtggg 3120 tttggagatg tacggctgag atgcctctat gcgaagggga tttcgtggtg aaacgagatg 3180 ggagtagagc aacgcccgtg gaagatgctt caaacttcca cacttttgag caacgatcgg 3240 cagtagtaag gtagacgatt tcaagatcaa agcatatgaa gataaacaac atcaacaaca 3300 aaatttgttg gggttctata gagagaaaca gagctacata catacactgt tttgtatcta 3360 ccatctgaga tgatgaaaag atgaaaaact aaagaatgcc ccggcgccaa cgccaggaca 3420 cgccgcgcgc gcgtcacccg agccatctct tgacccagcc ggcgctgtat atttacacac 3480 gttgcagcat cgatcaccac ctgttcgatc gcgtcgccgt caccaagctt ggcgcgccgg 3540 tcacccggtc cgggcctaga aggccacccg gggctcggcg ttaggctttt agtccgctag 3600 gtggctagac cgaggttagg acgctagttc actaggggcc tagtggtgtt ttaaggatgg 3660 gcaagtttgt acaaaaaagc aggctggcgc cggaaccaat tcagtcgagt gcggccgcca 3720 gtgtgatgga tatctcgacc cgggtgattg cggttacatc atgtacggaa aaataattct 3780 aatccttgat ttaaatttga acttgactat ttatttattc tttatttcat tttgtaaatc 3840 attttatgta tctcctggca agcaatttta tccaccttgc accaacacct tcgggttcca 3900 taatcaaacc accttaactt cacaccatgc tgtaactcac accgcccagc atctccaatg 3960 tgaaagaagc taaaatttaa taaacaatca tacgaagcag tgacaaaata ccagatggta 4020 ttaatgcttc gataaaatta attggaaagt ataaaatggt agaaaataat aaattataat 4080 taatttaagt aagataaaaa ataattaaaa actaaaatgt taaaatttta aaaaaattat 4140 tttaaataat atttaaaaac attaaaaatc attttaaaaa atttatttat agaacaatta 4200 aataaatatt tcagctaata aaaaacaaaa gcttacctag ccttagaaga caacttgtcc 4260 aacaattaga tgatacccat tgcccttacg ttttctttaa catcaattat tgtttttgtc 4320 aacaagctat cttttagttt tattttattg gtaaaaaata tgtcgccttc aagttgcatc 4380 atttaacaca tctcgtcatt agaaaaataa aactcttccc taaacgatta gtagaaaaaa 4440 tcattcgata ataaataaga aagaaaaatt agaaaaaaat aacttcattt taaaaaaatc 4500 attaaggcta tattttttaa atgactaatt ttatatagac tgtaactaaa agtatacaat 4560 ttattatgct atgtatctta aagaattact tataaaaatc tacggaagaa tatcttacaa 4620 agtgaaaaac aaatgagaaa gaatttagtg ggatgattat gattttattt gaaaattgaa 4680 aaaataatta ttaaagactt tagtggagta agaaagcttt cctattagtc ttttcttatc 4740 cataaaaaaa aaaaaaaaaa tctagcgtga cagcttttcc atagatttta ataatgtaaa 4800 atactggtag cagccgaccg ttcaggtaat ggacactgtg gtcctaactt gcaacgggtg 4860 cgggcccaat ttaataacgc cgtggtaacg gataaagcca agcgtgaagc ggtgaaggta 4920 catctctgac tccgtcaaga ttacgaaacc gtcaactacg aaggactccc cgaaatatca 4980 tctgtgtcat aaacaccaag tcacaccata catgggcacg cgtcacaata tgattggaga 5040 acggttccac cgcatatgct ataaaatgcc cccacacccc tcgaccctaa tcgcacttca 5100 attgcaatca aattagttca ttctctttgc gcagttccct acctctcctt tcaaggttcg 5160 tagatttctt ccgttttttt ttcttcttct ttattgtttg ttctacatca gcatgatgtt 5220 gatttgattg tgttttctat cgtttcatcg attataaatt ttcataatca gaagattcag 5280 cttttattaa tgcaagaacg tccttaattg atgattttat aaccgtaaat taggtctaat 5340 tagagttttt ttcataaaga ttttcagatc cgtttacaac aagccttaat tgttgattct 5400 gtagtcgtag attaaggttt ttttcatgaa ctacttcaga tccgttaaac aacagcctta 5460 tttgttgata cttcagtcgt ttttcaagaa attgttcaga tccgttgata aaagccttat 5520 tcgttgattc tgtatggtat ttcaagagat attgctcagg tcctttagca actaccttat 5580 ttgttgattc tgtggccata gattaggatt ttttttcacg aaattgcttc ttgaaattac 5640 gtgatggatt ttgattctga tttatcttgt gattgttgac tctacagcca tggcccacag 5700 caagcacggc ctgaaggagg agatgaccat gaagtaccac atggagggct gcgtgaacgg 5760 ccacaagttc gtgatcaccg gcgagggcat cggctacccc ttcaagggca agcagaccat 5820 caacctgtgc gtgatcgagg gcggccccct gcccttcagc gaggacatcc tgagcgccgg 5880 cttcaagtac ggcgaccgga tcttcaccga gtacccccag gacatcgtgg actacttcaa 5940 gaacagctgc cccgccggct acacctgggg ccggagcttc ctgttcgagg acggcgccgt 6000 gtgcatctgt aacgtggaca tcaccgtgag cgtgaaggag aactgcatct accacaagag 6060 catcttcaac ggcgtgaact tccccgccga cggccccgtg atgaagaaga tgaccaccaa 6120 ctgggaggcc agctgcgaga agatcatgcc cgtgcctaag cagggcatcc tgaagggcga 6180 cgtgagcatg tacctgctgc tgaaggacgg cggccggtac cggtgccagt tcgacaccgt 6240 gtacaaggcc aagagcgtgc ccagcaagat gcccgagtgg cacttcatcc agcacaagct 6300 gctgcgggag gaccggagcg acgccaagaa ccagaagtgg cagctgaccg agcacgccat 6360 cgccttcccc agcgccctgg cctgagagct cgaatttccc cgatcgttca aacatttggc 6420 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc 6480 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat 6540 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat 6600 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat 6660 tctagtggcc ggcccagctg atatctagac ccagctttct tgtacaaagt ggccgttaac 6720 ggatcggcca gaatggcccg gaccgggtta ccgaattcga gctcggtacc ctgggatccg 6780 atatcgatgg gccctggccg aagcttggtc acccggtccg ggcctagaag gccagcttcg 6840 gccgccccgg gcaactttat tatacaaagt tgatggtacg gatccgtcga cggcgcgccg 6900 cggacctgaa ttccagcaca ctggcggccg ttagtctaaa aacggcccct aactaacgct 6960 tagttgcccg ctagattagt cccccgttag tgcctagtta gacacacgac gaactagggg 7020 ctactagtaa cggccgccag tgtgctcggt acggccagaa tggccatctg gattcagcag 7080 ggggtgattg cggttacatc atgtacggaa aaataattct aatccttgat ttaaatttga 7140 acttgactat ttatttattc tttatttcat tttgtaaatc attttatgta tctcctggca 7200 agcaatttta tccaccttgc accaacacct tcgggttcca taatcaaacc accttaactt 7260 cacaccatgc tgtaactcac accgcccagc atctccaatg tgaaagaagc taaaatttaa 7320 taaacaatca tacgaagcag tgacaaaata ccagatggta ttaatgcttc gataaaatta 7380 attggaaagt ataaaatggt agaaaataat aaattataat taatttaagt aagataaaaa 7440 ataattaaaa actaaaatgt taaaatttta aaaaaattat tttaaataat atttaaaaac 7500 attaaaaatc attttaaaaa atttatttat agaacaatta aataaatatt tcagctaata 7560 aaaaacaaaa gcttacctag ccttagaaga caacttgtcc aacaattaga tgatacccat 7620 tgcccttacg ttttctttaa catcaattat tgtttttgtc aacaagctat cttttagttt 7680 tattttattg gtaaaaaata tgtcgccttc aagttgcatc atttaacaca tctcgtcatt 7740 agaaaaataa aactcttccc taaacgatta gtagaaaaaa tcattcgata ataaataaga 7800 aagaaaaatt agaaaaaaat aacttcattt taaaaaaatc attaaggcta tattttttaa 7860 atgactaatt ttatatagac tgtaactaaa agtatacaat ttattatgct atgtatctta 7920 aagaattact tataaaaatc tacggaagaa tatcttacaa agtgaaaaac aaatgagaaa 7980 gaatttagtg ggatgattat gattttattt gaaaattgaa aaaataatta ttaaagactt 8040 tagtggagta agaaagcttt cctattagtc ttttcttatc cataaaaaaa aaaaaaaaaa 8100 tctagcgtga cagcttttcc atagatttta ataatgtaaa atactggtag cagccgaccg 8160 ttcaggtaat ggacactgtg gtcctaactt gcaacgggtg cgggcccaat ttaataacgc 8220 cgtggtaacg gataaagcca agcgtgaagc ggtgaaggta catctctgac tccgtcaaga 8280 ttacgaaacc gtcaactacg aaggactccc cgaaatatca tctgtgtcat aaacaccaag 8340 tcacaccata catgggcacg cgtcacaata tgattggaga acggttccac cgcatatgct 8400 ataaaatgcc cccacacccc tcgaccctaa tcgcacttca attgcaatca aattagttca 8460 ttctctttgc gcagttccct acctctcctt tcaaggttcg tagatttctt ccgttttttt 8520 ttcttcttct ttattgtttg ttctacatca gcatgatgtt gatttgattg tgttttctat 8580 cgtttcatcg attataaatt ttcataatca gaagattcag cttttattaa tgcaagaacg 8640 tccttaattg atgattttat aaccgtaaat taggtctaat tagagttttt ttcataaaga 8700 ttttcagatc cgtttacaac aagccttaat tgttgattct gtagtcgtag attaaggttt 8760 ttttcatgaa ctacttcaga tccgttaaac aacagcctta tttgttgata cttcagtcgt 8820 ttttcaagaa attgttcaga tccgttgata aaagccttat tcgttgattc tgtatggtat 8880 ttcaagagat attgctcagg tcctttagca actaccttat ttgttgattc tgtggccata 8940 gattaggatt ttttttcacg aaattgcttc ttgaaattac gtgatggatt ttgattctga 9000 tttatcttgt gattgttgac tctacagcca tggctgcaac tactcttaca tctgctttac 9060 ctggtgcttt cagttcatcc cagagaccaa gtgctccttt caacttacaa agatcaccta 9120 gagtgcttag aaggttcaac agaaagacag gacgtcaacc tcgtggactt gttcgtgctg 9180 caaaggctca agcggcgcaa aacctgccag agaaattcga tgaaacaaga ctctgggagg 9240 ccctcagggg atttggggtt agtccaagta gggttactta cgctcccgtc ggattcggag 9300 attaccactg gaccgtcaca gatgaagacg ggagaccttg gttcgcaact gtcagtgacc 9360 tcgaacacaa agagcattgc gggcagggag cacaagcagc ccttaaagga ctcagacaag 9420 ctatggacac tgccctcgca cttagagata gggacggact cagattcgtg gttgctccag 9480 tggcagcaac agatgggggt ggccctgtcc ttccccttga cgctaggtat gcactcacag 9540 tcttccctca cgtcccagga agaacaggag agttcggaca aagactcaca gaggcagaga 9600 gggacagact gcttgctctt ctggcagaac tccacggaag aacacctcct gaaacaaccc 9660 caccagccga tatggaacca ccaggacttc ctggtgttag agcagcactc gccgaaagtg 9720 aaggaccttg gagtggagga ccatttgcag aacccgctag acttcttctc agagaacacg 9780 aggccacact gcacgcaaga ctcgctgaat tcgaagccct cgtcgcaaga gttaagggtc 9840 gtggagcacc tcttgtcgtt acccacggag aaccacaccc aggcaacttg atcctcggag 9900 aggaaggcta tctcctcgtg gactgggata cagttggact ggctccagct gaaagggatt 9960 tgagtctcat ctcagacgac ccagctgacc tcgcaagata cgcagagctt acaggaagaa 10020 ctccagaccc agacgcactc gcactctata gactgcgttg gagtctcctt gatgtggctg 10080 agttcgtcga gtggttcagg gctgaacacc aaagaaccca agacaccgag agtgcatgga 10140 aggggttcac tgacacactt gggcaattgg ctgctggtgg atcagctgca tagcaaactt 10200 atctctgtta tgaatcagaa gaagttcatg tctcgtttca tttaaaactt tggtggtttg 10260 tgttttgggg ccttgtaaag cccctgatga ataattgttc aactatgttt ccgttcctgt 10320 gttatacctt tctttctaat gagtaatgac atcaaacttc ttctgtattg aaattatgtc 10380 cttgtgagtc tctttatcat cgtttcgtct ttacattata tgtgctactt ttgtctaatg 10440 agcctgaaaa gtggctccaa tggtacgcac tggaagattt gttggcttct ggtagatata 10500 gcgacagtgt tgagcttgta atatcatgtc tcttattgct aaattagttc ctttcttaac 10560 agaaaccttc aaagtttttg tttttgtttt catttaccta atgtacacat acgctggcca 10620 tgactaacaa catgtccagg cttagagcat atttttttct agcttaaatt gttaacttgt 10680 cattcagtaa aatccgagaa ttgtgaagct ctaattgaag ctaattcgtt ttataaagtc 10740 agttaaaaag tatactaaat tatccaactt ttcttcaaaa tctcaaaatt ctatgacaaa 10800 acgatagtct ttgtttatgt cagtaccaca aagaggtgga aaaaaacacc aaaaaaacaa 10860 taagcaaact atacactgag aagaaaaata aaagagagct caatagatgt tttatactaa 10920 cggtagatta gatcaaagat ccaagcttta ctctacatag agcagaaccc agaatccctt 10980 catatctctt ttattctagc accgataatc tactgaaaag aagacactta gagctctgtc 11040 tctttgtcaa agaagtccca gccgtcatcc agaagctcct tacgttcatt aacagaggcc 11100 gcaattggat cgggtttact tattttgtgg gtatctatac ttttattaga tttttaatca 11160 ggctcctgat ttctttttat ttcgattgaa ttcctgaact tgtattattc agtagatcga 11220 ataaattata aaaagataaa atcataaaat aatattttat cctatcaatc atattaaagc 11280 aatgaatatg taaaattaat cttatcttta ttttaaaaaa tcatataggt ttagtatttt 11340 tttaaaaata aagataggat tagttttact attcactgct tattactttt aaaaaaatca 11400 taaaggttta gtattttttt aaaataaata taggaatagt tttactattc actgctttaa 11460 tagaaaaata gtttaaaatt taagatagtt ttaatcccag catttgccac gtttgaacgt 11520 gagccgaaac gatgtcgtta cattatctta acctagctga aacgatgtcg tcataatatc 11580 gccaaatgcc aactggacta cgtcgaaccc acaaatccca caaagcgcgt gaaatcaaat 11640 cgctcaaacc acaaaaaaga acaacgcgtt tgttacacgc tcaatcccac gcgagtagag 11700 cacagtaacc ttcaaataag cgaatggggc ataatcagaa atccgaaata aacctagggg 11760 cattatcgga aatgaaaagt agctcactca atataaaaat ctaggaaccc tagttttcgt 11820 tatcactctg tgctccctcg ctctatttct cagtctctgt gtttgcggct gaggattccg 11880 aacgagtgac cttcttcgtt tctcgcaaag gtaacagcct ctgctcttgt ctcttcgatt 11940 cgatctatgc ctgtctctta tttacgatga tgtttcttcg gttatgtttt tttatttatg 12000 ctttatgctg ttgatgttcg gttgtttgtt tcgctttgtt tttgtggttc agttttttag 12060 gattcttttg gtttttgaat cgattaatcg gaagagattt tcgagttatt tggtgtgttg 12120 gaggtgaatc ttttttttga ggtcatagat ctgttgtatt tgtgttataa acatgcgact 12180 ttgtatgatt ttttacgagg ttatgatgtt ctggttgttt tattatgaat ctgttgagac 12240 agaaccatga tttttgttga tgttcgttta cactattaaa ggtttgtttt aacaggatta 12300 aaagtttttt aagcatgttg aaggagtctt gtagatatgt aaccgtcgat agtttttttg 12360 tgggtttgtt cacatgttat caagcttaat cttttactat gtatgcgacc atatctggat 12420 ccagcaaagg cgatttttta attccttgtg aaacttttgt aatatgaagt tgaaattttg 12480 ttattggtaa actataaatg tgtgaagttg gagtatacct ttaccttctt atttggcttt 12540 gtgatagttt aatttatatg tattttgagt tctgacttgt atttctttga attgattcta 12600 gtttaagtaa tcatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg 12660 cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 12720 ccctacgagg gccacaacac cgtgaagctg aaggtgacga agggcggccc cctgcccttc 12780 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc 12840 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 12900 atgaacttcg aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc 12960 tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg 13020 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg 13080 ctgaagggcg agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag 13140 ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac 13200 gccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc 13260 accgagggcc gccaccacct gttcctgtag ttttgtgatc tgatgataag tggttggttc 13320 gtgtctcatg cacttgggag gtgatctatt tcacctggtg tagtttgtgt ttccgtcagt 13380 tggaaaaact tatccctatc gatttcgttt tcattttctg cttttctttt atgtaccttc 13440 gtttgggctt gtaacgggcc tttgtatttc aactctcaat aataatccaa gtgcatgtta 13500 aacaatttgt catctgtttc ggctttgata tactactggt gaagatgggc cgtactactg 13560 catcacaacg aaaaataata ataagatgaa aaacttgaag tggaaaaaaa aaaaaacttg 13620 aatgttcact actactcatt gaccataatg tttaacatac atagctcaat agtatttttg 13680 tgaatatggc aacacaaaca gtccaaaaca attgtctctt actataccaa accaagggcg 13740 ccgcttgttt gccactcttt gtgtgcaata gtgtgattac cacatctcca cattcaatat 13800 attccctgaa ttatctgacg attttgatgg ctcactgttt tcccaagtct tgaattgtct 13860 tctgtgcgcc agtcaaatgc atatgtgttg agtttatctt ttaaatatca agcttttgtt 13920 tttaactttt gtttgtaacc aaaaactcac agtaggagtt tgatcacata attttatgtt 13980 tgcctttgca atttctagtg agtctttgat taaaagcttg aaaagaaaat gcagccaagc 14040 ttaccaagta agttatgtgt attaaccaga ggaagagaga atcttgcaaa atttcaacaa 14100 acacaaaaag aagtattact acgattggtg gagaaagaaa acgattccaa atcttgaact 14160 gttgttgtaa aagcatagca gaaagtggga gacaaccgaa atagaaatga ctataactta 14220 atttaatgtt atcattataa tttcttctag caaatattta gaaagtaaat atcacatcaa 14280 cctttaatgt aattaagctt tctctttttg attcatgtga gatgaaaaga aaaaaaagaa 14340 gagaaaagtg tagaaaacac atcatttcta agctgaagct gaggtacaaa ttaattaata 14400 agtgactagg gtcacgtgac cctagtcact tatgattccg atgacttcgt aggttcctag 14460 ctcaagccgc tcgtgtccaa gcgtcactta cgattagcta atgattacgg catctaggac 14520 cgactagttc ttatgtgctt ctagtctcca aatgtggttg atagttattt tgctctaaga 14580 tcaacagtaa tgaagtataa atcatcgttg tggtgtgcta ctcggttaat tgagcattaa 14640 cacacacaaa catgacgagg atggtataat ctccaaaaat gtgtactttg ttaggtggga 14700 ccctatagcc ttgattaatg tgctatgtta ggcatgcctg gaaacgtgtg acgcatatgt 14760 tttgtgaacc tgttgatatt atatgtgctt ttatattacc atattttatt aaaatactaa 14820 tatttattac tagtaagata taacattcta tctagcttaa aaactaacca taaatattcc 14880 ataataacta gatttaccaa actaatatac taaatataca taataaatac aaaattaaca 14940 agacaataat caatatttat gagcttaata tatttagaca ttatggttgg tcgacgataa 15000 tcatgctaac ttttcgtaat tgcttgattg aaatatgctt agaataatgc ctctttgttc 15060 tacatggcaa atagggacca ttatggtgta acaccctggg aaccacaaac accccgaaat 15120 gctactaaac tacacaacta accttcatat ataaaatttc gacagcatct cctttgaaaa 15180 tttgcataga cgtggaagca acagagtata aacagatcta gctagctaag tttaaacggc 15240 cggccaaacg tgcaagcgct actagacaat tcagtacatt aaaaacgtcc gcaatgtgtt 15300 attaagttgt ctaagcgtca atttatttac aattgaatat atcctgcca 15349 <210> 161 <211> 15440 <212> DNA <213> Artificial Sequence <220> <223> RV026527 T-DNA <400> 161 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccaagc ttggatctcc tgcaggatct ggccggccgg atgggcggat 1320 ctcgtacctc aactttgtat agaaaagttg ggccagaatg gcccggaccg ggttaccaag 1380 cggccgcgaa ttccagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcg 1440 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa 1500 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat 1560 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa 1620 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta 1680 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca 1740 tttcatttgg agaggaccca tggaacatca tcatcagcag cagttccacg gtggccagcc 1800 gcagctgatg agtgaaaatg aagagatgaa caacaatggg agcgggggtg ggagtagtaa 1860 gagcagtttt ctttgcagac agtcgagcac aaggtggata cccactactg atcagataag 1920 gatactgaag gatctatact acaacagtgg actgagatcc ccaaacgccg atcagatcca 1980 aagaatatcc gccagactga ggcagtacgg aaagatcgag ggtaagaatg ttttctactg 2040 gtttcaaaat cacaaagccc gagagaggca gaagaagaga ctcaccgtcg atattgctgc 2100 agcggcaact accaacaaat tctccaacaa cactaatatg tcttcttctt cttctccctc 2160 agggatttct cctgcttctc atgcagtgta tgcaggatta gggcagatgg ggagcaatgg 2220 cggtggatca gtcgtgatgg agagaacttc ttacatggag tgttccttgc caggtgggat 2280 gccgatgccc gtacctgaac ccaatgtggg agtcggagtg ggatgggtga gtgttgaaac 2340 aggagctact actatgcaac aattctacta ccatcaacag agaccaacca caccctttcc 2400 tgctcctcat catcaccacc attactacga cgacggttac tattccaata ataacaaact 2460 tcaagcccat cacctacata tacgcgagga aaatcaacat ccagaagatg acgacgaaga 2520 agaagaagaa gaagaagaag atgaagagat agaaacactt ccattgttcc caatccactc 2580 acaggagaca cagtcttact tcctggacca acaacatgca atccacaatc aagaaagcaa 2640 taacatggaa acctctttgg agctcaccct aaactcctac tatgctgctt ccagtagcag 2700 tactccacat ggatcagagc atgattaagg tacctgacat cttatagtct gcaacctctc 2760 gtgtctgaat tcctatcttt atcaagtgtt attgcttcca cgactatagg acagctttcg 2820 tcgaaaggtt ttgctcatgt gatctcgaag gattcatcta gtctgatttt tcgtgacttg 2880 tatcggtttt attggattca tccaacatat atcaataaaa aatgagttgt gtttcctttc 2940 ttcctagttc agttaaaatt atttccctcc tgcgcttgtg ctgtaattgt ctgtgtacct 3000 gttgtttgtg actgtgttag ttcccttgga tatgatttcg tatttgatat gtacatggag 3060 atagcttagc ttcattattg gagtatgaag ttagtatgac atagtcactc tcctggaaaa 3120 ttgacactgc aaaccatatt tttattctga accacaaatc ctagtcagtc cgctggcata 3180 tgccgtccgt ttgctgaatc cagaacgtgg gtttggagat gtacggctga gatgcctcta 3240 tgcgaagggg atttcgtggt gaaacgagat gggagtagag caacgcccgt ggaagatgct 3300 tcaaacttcc acacttttga gcaacgatcg gcagtagtaa ggtagacgat ttcaagatca 3360 aagcatatga agataaacaa catcaacaac aaaatttgtt ggggttctat agagagaaac 3420 agagctacat acatacactg ttttgtatct accatctgag atgatgaaaa gatgaaaaac 3480 taaagaatgc cccggcgcca acgccaggac acgccgcgcg cgcgtcaccc gagccatctc 3540 ttgacccagc cggcgctgta tatttacaca cgttgcagca tcgatcacca cctgttcgat 3600 cgcgtcgccg tcaccaagct tggcgcgccg gtcacccggt ccgggcctag aaggccaccc 3660 ggggctcggc gttaggcttt tagtccgcta ggtggctaga ccgaggttag gacgctagtt 3720 cactaggggc ctagtggtgt tttaaggatg ggcaagtttg tacaaaaaag caggctggcg 3780 ccggaaccaa ttcagtcgag tgcggccgcc agtgtgatgg atatctcgac ccgggtgatt 3840 gcggttacat catgtacgga aaaataattc taatccttga tttaaatttg aacttgacta 3900 tttatttatt ctttatttca ttttgtaaat cattttatgt atctcctggc aagcaatttt 3960 atccaccttg caccaacacc ttcgggttcc ataatcaaac caccttaact tcacaccatg 4020 ctgtaactca caccgcccag catctccaat gtgaaagaag ctaaaattta ataaacaatc 4080 atacgaagca gtgacaaaat accagatggt attaatgctt cgataaaatt aattggaaag 4140 tataaaatgg tagaaaataa taaattataa ttaatttaag taagataaaa aataattaaa 4200 aactaaaatg ttaaaatttt aaaaaaatta ttttaaataa tatttaaaaa cattaaaaat 4260 cattttaaaa aatttattta tagaacaatt aaataaatat ttcagctaat aaaaaacaaa 4320 agcttaccta gccttagaag acaacttgtc caacaattag atgataccca ttgcccttac 4380 gttttcttta acatcaatta ttgtttttgt caacaagcta tcttttagtt ttattttatt 4440 ggtaaaaaat atgtcgcctt caagttgcat catttaacac atctcgtcat tagaaaaata 4500 aaactcttcc ctaaacgatt agtagaaaaa atcattcgat aataaataag aaagaaaaat 4560 tagaaaaaaa taacttcatt ttaaaaaaat cattaaggct atatttttta aatgactaat 4620 tttatataga ctgtaactaa aagtatacaa tttattatgc tatgtatctt aaagaattac 4680 ttataaaaat ctacggaaga atatcttaca aagtgaaaaa caaatgagaa agaatttagt 4740 gggatgatta tgattttatt tgaaaattga aaaaataatt attaaagact ttagtggagt 4800 aagaaagctt tcctattagt cttttcttat ccataaaaaa aaaaaaaaaa atctagcgtg 4860 acagcttttc catagatttt aataatgtaa aatactggta gcagccgacc gttcaggtaa 4920 tggacactgt ggtcctaact tgcaacgggt gcgggcccaa tttaataacg ccgtggtaac 4980 ggataaagcc aagcgtgaag cggtgaaggt acatctctga ctccgtcaag attacgaaac 5040 cgtcaactac gaaggactcc ccgaaatatc atctgtgtca taaacaccaa gtcacaccat 5100 acatgggcac gcgtcacaat atgattggag aacggttcca ccgcatatgc tataaaatgc 5160 ccccacaccc ctcgacccta atcgcacttc aattgcaatc aaattagttc attctctttg 5220 cgcagttccc tacctctcct ttcaaggttc gtagatttct tccgtttttt tttcttcttc 5280 tttattgttt gttctacatc agcatgatgt tgatttgatt gtgttttcta tcgtttcatc 5340 gattataaat tttcataatc agaagattca gcttttatta atgcaagaac gtccttaatt 5400 gatgatttta taaccgtaaa ttaggtctaa ttagagtttt tttcataaag attttcagat 5460 ccgtttacaa caagccttaa ttgttgattc tgtagtcgta gattaaggtt tttttcatga 5520 actacttcag atccgttaaa caacagcctt atttgttgat acttcagtcg tttttcaaga 5580 aattgttcag atccgttgat aaaagcctta ttcgttgatt ctgtatggta tttcaagaga 5640 tattgctcag gtcctttagc aactacctta tttgttgatt ctgtggccat agattaggat 5700 tttttttcac gaaattgctt cttgaaatta cgtgatggat tttgattctg atttatcttg 5760 tgattgttga ctctacagcc atggcccaca gcaagcacgg cctgaaggag gagatgacca 5820 tgaagtacca catggagggc tgcgtgaacg gccacaagtt cgtgatcacc ggcgagggca 5880 tcggctaccc cttcaagggc aagcagacca tcaacctgtg cgtgatcgag ggcggccccc 5940 tgcccttcag cgaggacatc ctgagcgccg gcttcaagta cggcgaccgg atcttcaccg 6000 agtaccccca ggacatcgtg gactacttca agaacagctg ccccgccggc tacacctggg 6060 gccggagctt cctgttcgag gacggcgccg tgtgcatctg taacgtggac atcaccgtga 6120 gcgtgaagga gaactgcatc taccacaaga gcatcttcaa cggcgtgaac ttccccgccg 6180 acggccccgt gatgaagaag atgaccacca actgggaggc cagctgcgag aagatcatgc 6240 ccgtgcctaa gcagggcatc ctgaagggcg acgtgagcat gtacctgctg ctgaaggacg 6300 gcggccggta ccggtgccag ttcgacaccg tgtacaaggc caagagcgtg cccagcaaga 6360 tgcccgagtg gcacttcatc cagcacaagc tgctgcggga ggaccggagc gacgccaaga 6420 accagaagtg gcagctgacc gagcacgcca tcgccttccc cagcgccctg gcctgagagc 6480 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt 6540 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt 6600 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta 6660 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc 6720 gcggtgtcat ctatgttact agatcgggaa ttctagtggc cggcccagct gatatctaga 6780 cccagctttc ttgtacaaag tggccgttaa cggatcggcc agaatggccc ggaccgggtt 6840 accgaattcg agctcggtac cctgggatcc gatatcgatg ggccctggcc gaagcttggt 6900 cacccggtcc gggcctagaa ggccagcttc ggccgccccg ggcaacttta ttatacaaag 6960 ttgatggtac ggatccgtcg acggcgcgcc gcggacctga attccagcac actggcggcc 7020 gttagtctaa aaacggcccc taactaacgc ttagttgccc gctagattag tcccccgtta 7080 gtgcctagtt agacacacga cgaactaggg gctactagta acggccgcca gtgtgctcgg 7140 tacggccaga atggccatct ggattcagca gggggtgatt gcggttacat catgtacgga 7200 aaaataattc taatccttga tttaaatttg aacttgacta tttatttatt ctttatttca 7260 ttttgtaaat cattttatgt atctcctggc aagcaatttt atccaccttg caccaacacc 7320 ttcgggttcc ataatcaaac caccttaact tcacaccatg ctgtaactca caccgcccag 7380 catctccaat gtgaaagaag ctaaaattta ataaacaatc atacgaagca gtgacaaaat 7440 accagatggt attaatgctt cgataaaatt aattggaaag tataaaatgg tagaaaataa 7500 taaattataa ttaatttaag taagataaaa aataattaaa aactaaaatg ttaaaatttt 7560 aaaaaaatta ttttaaataa tatttaaaaa cattaaaaat cattttaaaa aatttattta 7620 tagaacaatt aaataaatat ttcagctaat aaaaaacaaa agcttaccta gccttagaag 7680 acaacttgtc caacaattag atgataccca ttgcccttac gttttcttta acatcaatta 7740 ttgtttttgt caacaagcta tcttttagtt ttattttatt ggtaaaaaat atgtcgcctt 7800 caagttgcat catttaacac atctcgtcat tagaaaaata aaactcttcc ctaaacgatt 7860 agtagaaaaa atcattcgat aataaataag aaagaaaaat tagaaaaaaa taacttcatt 7920 ttaaaaaaat cattaaggct atatttttta aatgactaat tttatataga ctgtaactaa 7980 aagtatacaa tttattatgc tatgtatctt aaagaattac ttataaaaat ctacggaaga 8040 atatcttaca aagtgaaaaa caaatgagaa agaatttagt gggatgatta tgattttatt 8100 tgaaaattga aaaaataatt attaaagact ttagtggagt aagaaagctt tcctattagt 8160 cttttcttat ccataaaaaa aaaaaaaaaa atctagcgtg acagcttttc catagatttt 8220 aataatgtaa aatactggta gcagccgacc gttcaggtaa tggacactgt ggtcctaact 8280 tgcaacgggt gcgggcccaa tttaataacg ccgtggtaac ggataaagcc aagcgtgaag 8340 cggtgaaggt acatctctga ctccgtcaag attacgaaac cgtcaactac gaaggactcc 8400 ccgaaatatc atctgtgtca taaacaccaa gtcacaccat acatgggcac gcgtcacaat 8460 atgattggag aacggttcca ccgcatatgc tataaaatgc ccccacaccc ctcgacccta 8520 atcgcacttc aattgcaatc aaattagttc attctctttg cgcagttccc tacctctcct 8580 ttcaaggttc gtagatttct tccgtttttt tttcttcttc tttattgttt gttctacatc 8640 agcatgatgt tgatttgatt gtgttttcta tcgtttcatc gattataaat tttcataatc 8700 agaagattca gcttttatta atgcaagaac gtccttaatt gatgatttta taaccgtaaa 8760 ttaggtctaa ttagagtttt tttcataaag attttcagat ccgtttacaa caagccttaa 8820 ttgttgattc tgtagtcgta gattaaggtt tttttcatga actacttcag atccgttaaa 8880 caacagcctt atttgttgat acttcagtcg tttttcaaga aattgttcag atccgttgat 8940 aaaagcctta ttcgttgatt ctgtatggta tttcaagaga tattgctcag gtcctttagc 9000 aactacctta tttgttgatt ctgtggccat agattaggat tttttttcac gaaattgctt 9060 cttgaaatta cgtgatggat tttgattctg atttatcttg tgattgttga ctctacagcc 9120 atggctgcaa ctactcttac atctgcttta cctggtgctt tcagttcatc ccagagacca 9180 agtgctcctt tcaacttaca aagatcacct agagtgctta gaaggttcaa cagaaagaca 9240 ggacgtcaac ctcgtggact tgttcgtgct gcaaaggctc aagcggcgca aaacctgcca 9300 gagaaattcg atgaaacaag actctgggag gccctcaggg gatttggggt tagtccaagt 9360 agggttactt acgctcccgt cggattcgga gattaccact ggaccgtcac agatgaagac 9420 gggagacctt ggttcgcaac tgtcagtgac ctcgaacaca aagagcattg cgggcaggga 9480 gcacaagcag cccttaaagg actcagacaa gctatggaca ctgccctcgc acttagagat 9540 agggacggac tcagattcgt ggttgctcca gtggcagcaa cagatggggg tggccctgtc 9600 cttccccttg acgctaggta tgcactcaca gtcttccctc acgtcccagg aagaacagga 9660 gagttcggac aaagactcac agaggcagag agggacagac tgcttgctct tctggcagaa 9720 ctccacggaa gaacacctcc tgaaacaacc ccaccagccg atatggaacc accaggactt 9780 cctggtgtta gagcagcact cgccgaaagt gaaggacctt ggagtggagg accatttgca 9840 gaacccgcta gacttcttct cagagaacac gaggccacac tgcacgcaag actcgctgaa 9900 ttcgaagccc tcgtcgcaag agttaagggt cgtggagcac ctcttgtcgt tacccacgga 9960 gaaccacacc caggcaactt gatcctcgga gaggaaggct atctcctcgt ggactgggat 10020 acagttggac tggctccagc tgaaagggat ttgagtctca tctcagacga cccagctgac 10080 ctcgcaagat acgcagagct tacaggaaga actccagacc cagacgcact cgcactctat 10140 agactgcgtt ggagtctcct tgatgtggct gagttcgtcg agtggttcag ggctgaacac 10200 caaagaaccc aagacaccga gagtgcatgg aaggggttca ctgacacact tgggcaattg 10260 gctgctggtg gatcagctgc atagcaaact tatctctgtt atgaatcaga agaagttcat 10320 gtctcgtttc atttaaaact ttggtggttt gtgttttggg gccttgtaaa gcccctgatg 10380 aataattgtt caactatgtt tccgttcctg tgttatacct ttctttctaa tgagtaatga 10440 catcaaactt cttctgtatt gaaattatgt ccttgtgagt ctctttatca tcgtttcgtc 10500 tttacattat atgtgctact tttgtctaat gagcctgaaa agtggctcca atggtacgca 10560 ctggaagatt tgttggcttc tggtagatat agcgacagtg ttgagcttgt aatatcatgt 10620 ctcttattgc taaattagtt cctttcttaa cagaaacctt caaagttttt gtttttgttt 10680 tcatttacct aatgtacaca tacgctggcc atgactaaca acatgtccag gcttagagca 10740 tatttttttc tagcttaaat tgttaacttg tcattcagta aaatccgaga attgtgaagc 10800 tctaattgaa gctaattcgt tttataaagt cagttaaaaa gtatactaaa ttatccaact 10860 tttcttcaaa atctcaaaat tctatgacaa aacgatagtc tttgtttatg tcagtaccac 10920 aaagaggtgg aaaaaaacac caaaaaaaca ataagcaaac tatacactga gaagaaaaat 10980 aaaagagagc tcaatagatg ttttatacta acggtagatt agatcaaaga tccaagcttt 11040 actctacata gagcagaacc cagaatccct tcatatctct tttattctag caccgataat 11100 ctactgaaaa gaagacactt agagctctgt ctctttgtca aagaagtccc agccgtcatc 11160 cagaagctcc ttacgttcat taacagaggc cgcaattgga tcgggtttac ttattttgtg 11220 ggtatctata cttttattag atttttaatc aggctcctga tttcttttta tttcgattga 11280 attcctgaac ttgtattatt cagtagatcg aataaattat aaaaagataa aatcataaaa 11340 taatatttta tcctatcaat catattaaag caatgaatat gtaaaattaa tcttatcttt 11400 attttaaaaa atcatatagg tttagtattt ttttaaaaat aaagatagga ttagttttac 11460 tattcactgc ttattacttt taaaaaaatc ataaaggttt agtatttttt taaaataaat 11520 ataggaatag ttttactatt cactgcttta atagaaaaat agtttaaaat ttaagatagt 11580 tttaatccca gcatttgcca cgtttgaacg tgagccgaaa cgatgtcgtt acattatctt 11640 aacctagctg aaacgatgtc gtcataatat cgccaaatgc caactggact acgtcgaacc 11700 cacaaatccc acaaagcgcg tgaaatcaaa tcgctcaaac cacaaaaaag aacaacgcgt 11760 ttgttacacg ctcaatccca cgcgagtaga gcacagtaac cttcaaataa gcgaatgggg 11820 cataatcaga aatccgaaat aaacctaggg gcattatcgg aaatgaaaag tagctcactc 11880 aatataaaaa tctaggaacc ctagttttcg ttatcactct gtgctccctc gctctatttc 11940 tcagtctctg tgtttgcggc tgaggattcc gaacgagtga ccttcttcgt ttctcgcaaa 12000 ggtaacagcc tctgctcttg tctcttcgat tcgatctatg cctgtctctt atttacgatg 12060 atgtttcttc ggttatgttt ttttatttat gctttatgct gttgatgttc ggttgtttgt 12120 ttcgctttgt ttttgtggtt cagtttttta ggattctttt ggtttttgaa tcgattaatc 12180 ggaagagatt ttcgagttat ttggtgtgtt ggaggtgaat cttttttttg aggtcataga 12240 tctgttgtat ttgtgttata aacatgcgac tttgtatgat tttttacgag gttatgatgt 12300 tctggttgtt ttattatgaa tctgttgaga cagaaccatg atttttgttg atgttcgttt 12360 acactattaa aggtttgttt taacaggatt aaaagttttt taagcatgtt gaaggagtct 12420 tgtagatatg taaccgtcga tagttttttt gtgggtttgt tcacatgtta tcaagcttaa 12480 tcttttacta tgtatgcgac catatctgga tccagcaaag gcgatttttt aattccttgt 12540 gaaacttttg taatatgaag ttgaaatttt gttattggta aactataaat gtgtgaagtt 12600 ggagtatacc tttaccttct tatttggctt tgtgatagtt taatttatat gtattttgag 12660 ttctgacttg tatttctttg aattgattct agtttaagta atcatggcct cctccgagaa 12720 cgtcatcacc gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga 12780 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct 12840 gaaggtgacg aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca 12900 gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc 12960 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac 13020 cgtgacccag gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg 13080 cgtgaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc 13140 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa 13200 gctgaaggac ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc 13260 cgtgcagctg cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga 13320 ggactacacc atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta 13380 gttttgtgat ctgatgataa gtggttggtt cgtgtctcat gcacttggga ggtgatctat 13440 ttcacctggt gtagtttgtg tttccgtcag ttggaaaaac ttatccctat cgatttcgtt 13500 ttcattttct gcttttcttt tatgtacctt cgtttgggct tgtaacgggc ctttgtattt 13560 caactctcaa taataatcca agtgcatgtt aaacaatttg tcatctgttt cggctttgat 13620 atactactgg tgaagatggg ccgtactact gcatcacaac gaaaaataat aataagatga 13680 aaaacttgaa gtggaaaaaa aaaaaaactt gaatgttcac tactactcat tgaccataat 13740 gtttaacata catagctcaa tagtattttt gtgaatatgg caacacaaac agtccaaaac 13800 aattgtctct tactatacca aaccaagggc gccgcttgtt tgccactctt tgtgtgcaat 13860 agtgtgatta ccacatctcc acattcaata tattccctga attatctgac gattttgatg 13920 gctcactgtt ttcccaagtc ttgaattgtc ttctgtgcgc cagtcaaatg catatgtgtt 13980 gagtttatct tttaaatatc aagcttttgt ttttaacttt tgtttgtaac caaaaactca 14040 cagtaggagt ttgatcacat aattttatgt ttgcctttgc aatttctagt gagtctttga 14100 ttaaaagctt gaaaagaaaa tgcagccaag cttaccaagt aagttatgtg tattaaccag 14160 aggaagagag aatcttgcaa aatttcaaca aacacaaaaa gaagtattac tacgattggt 14220 ggagaaagaa aacgattcca aatcttgaac tgttgttgta aaagcatagc agaaagtggg 14280 agacaaccga aatagaaatg actataactt aatttaatgt tatcattata atttcttcta 14340 gcaaatattt agaaagtaaa tatcacatca acctttaatg taattaagct ttctcttttt 14400 gattcatgtg agatgaaaag aaaaaaaaga agagaaaagt gtagaaaaca catcatttct 14460 aagctgaagc tgaggtacaa attaattaat aagtgactag ggtcacgtga ccctagtcac 14520 ttatgattcc gatgacttcg taggttccta gctcaagccg ctcgtgtcca agcgtcactt 14580 acgattagct aatgattacg gcatctagga ccgactagtt cttatgtgct tctagtctcc 14640 aaatgtggtt gatagttatt ttgctctaag atcaacagta atgaagtata aatcatcgtt 14700 gtggtgtgct actcggttaa ttgagcatta acacacacaa acatgacgag gatggtataa 14760 tctccaaaaa tgtgtacttt gttaggtggg accctatagc cttgattaat gtgctatgtt 14820 aggcatgcct ggaaacgtgt gacgcatatg ttttgtgaac ctgttgatat tatatgtgct 14880 tttatattac catattttat taaaatacta atatttatta ctagtaagat ataacattct 14940 atctagctta aaaactaacc ataaatattc cataataact agatttacca aactaatata 15000 ctaaatatac ataataaata caaaattaac aagacaataa tcaatattta tgagcttaat 15060 atatttagac attatggttg gtcgacgata atcatgctaa cttttcgtaa ttgcttgatt 15120 gaaatatgct tagaataatg cctctttgtt ctacatggca aatagggacc attatggtgt 15180 aacaccctgg gaaccacaaa caccccgaaa tgctactaaa ctacacaact aaccttcata 15240 tataaaattt cgacagcatc tcctttgaaa atttgcatag acgtggaagc aacagagtat 15300 aaacagatct agctagctaa gtttaaacgg ccggccaaac gtgcaagcgc tactagacaa 15360 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttattta 15420 caattgaata tatcctgcca 15440 <210> 162 <211> 15202 <212> DNA <213> Artificial Sequence <220> <223> RV026529 T-DNA <400> 162 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gatcattctg ataactgccc aggtaacaac aacaactctg gttctggttc tggttctgga 1860 accaagtctg gttacatgtg cagacaatct aactctagat ggatcccaac cgctgaacaa 1920 atcagaatcc ttagagaact ttactactct aacggtgtta gatctccaac cgctgaacaa 1980 atccaaagaa tctctgctag acttagacaa tacggtaaga tcgaaggtaa gaacgttttt 2040 tactggtttc aaaaccataa ggctagagaa agacaaaaga agagacttac cgttgatctt 2100 gctgttcaaa gagctgctgc tacctctggt ggtcttccac caggtgttct taacgtttct 2160 catatcgctg gttctacctg cggttctgtt cttatggaat cttctcttat ggattgctgc 2220 tctacctctg ctcttgaaaa ccataacggt agaaaggaaa gagctaacgg tgatatgatc 2280 gttgaaagag aaagatctgc tccaggtaag gaaacccttc aactttttcc aatgcatcat 2340 tctccacaaa acgatgatac catcgctgat gattactttt ttaacgatga agaagaaaag 2400 ccagtttgca tggcttctct tgaacttacc cttaactctt ttgatagagt ttaccatcca 2460 gattctgatt ttagatctca ttcttcttaa ggtacctgac atcttatagt ctgcaacctc 2520 tcgtgtctga attcctatct ttatcaagtg ttattgcttc cacgactata ggacagcttt 2580 cgtcgaaagg ttttgctcat gtgatctcga aggattcatc tagtctgatt tttcgtgact 2640 tgtatcggtt ttattggatt catccaacat atatcaataa aaaatgagtt gtgtttcctt 2700 tcttcctagt tcagttaaaa ttatttccct cctgcgcttg tgctgtaatt gtctgtgtac 2760 ctgttgtttg tgactgtgtt agttcccttg gatatgattt cgtatttgat atgtacatgg 2820 agatagctta gcttcattat tggagtatga agttagtatg acatagtcac tctcctggaa 2880 aattgacact gcaaaccata tttttattct gaaccacaaa tcctagtcag tccgctggca 2940 tatgccgtcc gtttgctgaa tccagaacgt gggtttggag atgtacggct gagatgcctc 3000 tatgcgaagg ggatttcgtg gtgaaacgag atgggagtag agcaacgccc gtggaagatg 3060 cttcaaactt ccacactttt gagcaacgat cggcagtagt aaggtagacg atttcaagat 3120 caaagcatat gaagataaac aacatcaaca acaaaatttg ttggggttct atagagagaa 3180 acagagctac atacatacac tgttttgtat ctaccatctg agatgatgaa aagatgaaaa 3240 actaaagaat gccccggcgc caacgccagg acacgccgcg cgcgcgtcac ccgagccatc 3300 tcttgaccca gccggcgctg tatatttaca cacgttgcag catcgatcac cacctgttcg 3360 atcgcgtcgc cgtcaccaag cttggcgcgc cggtcacccg gtccgggcct agaaggccac 3420 ccggggctcg gcgttaggct tttagtccgc taggtggcta gaccgaggtt aggacgctag 3480 ttcactaggg gcctagtggt gttttaagga tgggcaagtt tgtacaaaaa agcaggctgg 3540 cgccggaacc aattcagtcg agtgcggccg ccagtgtgat ggatatctcg acccgggtga 3600 ttgcggttac atcatgtacg gaaaaataat tctaatcctt gatttaaatt tgaacttgac 3660 tatttattta ttctttattt cattttgtaa atcattttat gtatctcctg gcaagcaatt 3720 ttatccacct tgcaccaaca ccttcgggtt ccataatcaa accaccttaa cttcacacca 3780 tgctgtaact cacaccgccc agcatctcca atgtgaaaga agctaaaatt taataaacaa 3840 tcatacgaag cagtgacaaa ataccagatg gtattaatgc ttcgataaaa ttaattggaa 3900 agtataaaat ggtagaaaat aataaattat aattaattta agtaagataa aaaataatta 3960 aaaactaaaa tgttaaaatt ttaaaaaaat tattttaaat aatatttaaa aacattaaaa 4020 atcattttaa aaaatttatt tatagaacaa ttaaataaat atttcagcta ataaaaaaca 4080 aaagcttacc tagccttaga agacaacttg tccaacaatt agatgatacc cattgccctt 4140 acgttttctt taacatcaat tattgttttt gtcaacaagc tatcttttag ttttatttta 4200 ttggtaaaaa atatgtcgcc ttcaagttgc atcatttaac acatctcgtc attagaaaaa 4260 taaaactctt ccctaaacga ttagtagaaa aaatcattcg ataataaata agaaagaaaa 4320 attagaaaaa aataacttca ttttaaaaaa atcattaagg ctatattttt taaatgacta 4380 attttatata gactgtaact aaaagtatac aatttattat gctatgtatc ttaaagaatt 4440 acttataaaa atctacggaa gaatatctta caaagtgaaa aacaaatgag aaagaattta 4500 gtgggatgat tatgatttta tttgaaaatt gaaaaaataa ttattaaaga ctttagtgga 4560 gtaagaaagc tttcctatta gtcttttctt atccataaaa aaaaaaaaaa aaatctagcg 4620 tgacagcttt tccatagatt ttaataatgt aaaatactgg tagcagccga ccgttcaggt 4680 aatggacact gtggtcctaa cttgcaacgg gtgcgggccc aatttaataa cgccgtggta 4740 acggataaag ccaagcgtga agcggtgaag gtacatctct gactccgtca agattacgaa 4800 accgtcaact acgaaggact ccccgaaata tcatctgtgt cataaacacc aagtcacacc 4860 atacatgggc acgcgtcaca atatgattgg agaacggttc caccgcatat gctataaaat 4920 gcccccacac ccctcgaccc taatcgcact tcaattgcaa tcaaattagt tcattctctt 4980 tgcgcagttc cctacctctc ctttcaaggt tcgtagattt cttccgtttt tttttcttct 5040 tctttattgt ttgttctaca tcagcatgat gttgatttga ttgtgttttc tatcgtttca 5100 tcgattataa attttcataa tcagaagatt cagcttttat taatgcaaga acgtccttaa 5160 ttgatgattt tataaccgta aattaggtct aattagagtt tttttcataa agattttcag 5220 atccgtttac aacaagcctt aattgttgat tctgtagtcg tagattaagg tttttttcat 5280 gaactacttc agatccgtta aacaacagcc ttatttgttg atacttcagt cgtttttcaa 5340 gaaattgttc agatccgttg ataaaagcct tattcgttga ttctgtatgg tatttcaaga 5400 gatattgctc aggtccttta gcaactacct tatttgttga ttctgtggcc atagattagg 5460 attttttttc acgaaattgc ttcttgaaat tacgtgatgg attttgattc tgatttatct 5520 tgtgattgtt gactctacag ccatggccca cagcaagcac ggcctgaagg aggagatgac 5580 catgaagtac cacatggagg gctgcgtgaa cggccacaag ttcgtgatca ccggcgaggg 5640 catcggctac cccttcaagg gcaagcagac catcaacctg tgcgtgatcg agggcggccc 5700 cctgcccttc agcgaggaca tcctgagcgc cggcttcaag tacggcgacc ggatcttcac 5760 cgagtacccc caggacatcg tggactactt caagaacagc tgccccgccg gctacacctg 5820 gggccggagc ttcctgttcg aggacggcgc cgtgtgcatc tgtaacgtgg acatcaccgt 5880 gagcgtgaag gagaactgca tctaccacaa gagcatcttc aacggcgtga acttccccgc 5940 cgacggcccc gtgatgaaga agatgaccac caactgggag gccagctgcg agaagatcat 6000 gcccgtgcct aagcagggca tcctgaaggg cgacgtgagc atgtacctgc tgctgaagga 6060 cggcggccgg taccggtgcc agttcgacac cgtgtacaag gccaagagcg tgcccagcaa 6120 gatgcccgag tggcacttca tccagcacaa gctgctgcgg gaggaccgga gcgacgccaa 6180 gaaccagaag tggcagctga ccgagcacgc catcgccttc cccagcgccc tggcctgaga 6240 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg 6300 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa 6360 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat 6420 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc 6480 gcgcggtgtc atctatgtta ctagatcggg aattctagtg gccggcccag ctgatatcta 6540 gacccagctt tcttgtacaa agtggccgtt aacggatcgg ccagaatggc ccggaccggg 6600 ttaccgaatt cgagctcggt accctgggat ccgatatcga tgggccctgg ccgaagcttg 6660 gtcacccggt ccgggcctag aaggccagct tcggccgccc cgggcaactt tattatacaa 6720 agttgatggt acggatccgt cgacggcgcg ccgcggacct gaattccagc acactggcgg 6780 ccgttagtct aaaaacggcc cctaactaac gcttagttgc ccgctagatt agtcccccgt 6840 tagtgcctag ttagacacac gacgaactag gggctactag taacggccgc cagtgtgctc 6900 ggtacggcca gaatggccat ctggattcag cagggggtga ttgcggttac atcatgtacg 6960 gaaaaataat tctaatcctt gatttaaatt tgaacttgac tatttattta ttctttattt 7020 cattttgtaa atcattttat gtatctcctg gcaagcaatt ttatccacct tgcaccaaca 7080 ccttcgggtt ccataatcaa accaccttaa cttcacacca tgctgtaact cacaccgccc 7140 agcatctcca atgtgaaaga agctaaaatt taataaacaa tcatacgaag cagtgacaaa 7200 ataccagatg gtattaatgc ttcgataaaa ttaattggaa agtataaaat ggtagaaaat 7260 aataaattat aattaattta agtaagataa aaaataatta aaaactaaaa tgttaaaatt 7320 ttaaaaaaat tattttaaat aatatttaaa aacattaaaa atcattttaa aaaatttatt 7380 tatagaacaa ttaaataaat atttcagcta ataaaaaaca aaagcttacc tagccttaga 7440 agacaacttg tccaacaatt agatgatacc cattgccctt acgttttctt taacatcaat 7500 tattgttttt gtcaacaagc tatcttttag ttttatttta ttggtaaaaa atatgtcgcc 7560 ttcaagttgc atcatttaac acatctcgtc attagaaaaa taaaactctt ccctaaacga 7620 ttagtagaaa aaatcattcg ataataaata agaaagaaaa attagaaaaa aataacttca 7680 ttttaaaaaa atcattaagg ctatattttt taaatgacta attttatata gactgtaact 7740 aaaagtatac aatttattat gctatgtatc ttaaagaatt acttataaaa atctacggaa 7800 gaatatctta caaagtgaaa aacaaatgag aaagaattta gtgggatgat tatgatttta 7860 tttgaaaatt gaaaaaataa ttattaaaga ctttagtgga gtaagaaagc tttcctatta 7920 gtcttttctt atccataaaa aaaaaaaaaa aaatctagcg tgacagcttt tccatagatt 7980 ttaataatgt aaaatactgg tagcagccga ccgttcaggt aatggacact gtggtcctaa 8040 cttgcaacgg gtgcgggccc aatttaataa cgccgtggta acggataaag ccaagcgtga 8100 agcggtgaag gtacatctct gactccgtca agattacgaa accgtcaact acgaaggact 8160 ccccgaaata tcatctgtgt cataaacacc aagtcacacc atacatgggc acgcgtcaca 8220 atatgattgg agaacggttc caccgcatat gctataaaat gcccccacac ccctcgaccc 8280 taatcgcact tcaattgcaa tcaaattagt tcattctctt tgcgcagttc cctacctctc 8340 ctttcaaggt tcgtagattt cttccgtttt tttttcttct tctttattgt ttgttctaca 8400 tcagcatgat gttgatttga ttgtgttttc tatcgtttca tcgattataa attttcataa 8460 tcagaagatt cagcttttat taatgcaaga acgtccttaa ttgatgattt tataaccgta 8520 aattaggtct aattagagtt tttttcataa agattttcag atccgtttac aacaagcctt 8580 aattgttgat tctgtagtcg tagattaagg tttttttcat gaactacttc agatccgtta 8640 aacaacagcc ttatttgttg atacttcagt cgtttttcaa gaaattgttc agatccgttg 8700 ataaaagcct tattcgttga ttctgtatgg tatttcaaga gatattgctc aggtccttta 8760 gcaactacct tatttgttga ttctgtggcc atagattagg attttttttc acgaaattgc 8820 ttcttgaaat tacgtgatgg attttgattc tgatttatct tgtgattgtt gactctacag 8880 ccatggctgc aactactctt acatctgctt tacctggtgc tttcagttca tcccagagac 8940 caagtgctcc tttcaactta caaagatcac ctagagtgct tagaaggttc aacagaaaga 9000 caggacgtca acctcgtgga cttgttcgtg ctgcaaaggc tcaagcggcg caaaacctgc 9060 cagagaaatt cgatgaaaca agactctggg aggccctcag gggatttggg gttagtccaa 9120 gtagggttac ttacgctccc gtcggattcg gagattacca ctggaccgtc acagatgaag 9180 acgggagacc ttggttcgca actgtcagtg acctcgaaca caaagagcat tgcgggcagg 9240 gagcacaagc agcccttaaa ggactcagac aagctatgga cactgccctc gcacttagag 9300 atagggacgg actcagattc gtggttgctc cagtggcagc aacagatggg ggtggccctg 9360 tccttcccct tgacgctagg tatgcactca cagtcttccc tcacgtccca ggaagaacag 9420 gagagttcgg acaaagactc acagaggcag agagggacag actgcttgct cttctggcag 9480 aactccacgg aagaacacct cctgaaacaa ccccaccagc cgatatggaa ccaccaggac 9540 ttcctggtgt tagagcagca ctcgccgaaa gtgaaggacc ttggagtgga ggaccatttg 9600 cagaacccgc tagacttctt ctcagagaac acgaggccac actgcacgca agactcgctg 9660 aattcgaagc cctcgtcgca agagttaagg gtcgtggagc acctcttgtc gttacccacg 9720 gagaaccaca cccaggcaac ttgatcctcg gagaggaagg ctatctcctc gtggactggg 9780 atacagttgg actggctcca gctgaaaggg atttgagtct catctcagac gacccagctg 9840 acctcgcaag atacgcagag cttacaggaa gaactccaga cccagacgca ctcgcactct 9900 atagactgcg ttggagtctc cttgatgtgg ctgagttcgt cgagtggttc agggctgaac 9960 accaaagaac ccaagacacc gagagtgcat ggaaggggtt cactgacaca cttgggcaat 10020 tggctgctgg tggatcagct gcatagcaaa cttatctctg ttatgaatca gaagaagttc 10080 atgtctcgtt tcatttaaaa ctttggtggt ttgtgttttg gggccttgta aagcccctga 10140 tgaataattg ttcaactatg tttccgttcc tgtgttatac ctttctttct aatgagtaat 10200 gacatcaaac ttcttctgta ttgaaattat gtccttgtga gtctctttat catcgtttcg 10260 tctttacatt atatgtgcta cttttgtcta atgagcctga aaagtggctc caatggtacg 10320 cactggaaga tttgttggct tctggtagat atagcgacag tgttgagctt gtaatatcat 10380 gtctcttatt gctaaattag ttcctttctt aacagaaacc ttcaaagttt ttgtttttgt 10440 tttcatttac ctaatgtaca catacgctgg ccatgactaa caacatgtcc aggcttagag 10500 catatttttt tctagcttaa attgttaact tgtcattcag taaaatccga gaattgtgaa 10560 gctctaattg aagctaattc gttttataaa gtcagttaaa aagtatacta aattatccaa 10620 cttttcttca aaatctcaaa attctatgac aaaacgatag tctttgttta tgtcagtacc 10680 acaaagaggt ggaaaaaaac accaaaaaaa caataagcaa actatacact gagaagaaaa 10740 ataaaagaga gctcaataga tgttttatac taacggtaga ttagatcaaa gatccaagct 10800 ttactctaca tagagcagaa cccagaatcc cttcatatct cttttattct agcaccgata 10860 atctactgaa aagaagacac ttagagctct gtctctttgt caaagaagtc ccagccgtca 10920 tccagaagct ccttacgttc attaacagag gccgcaattg gatcgggttt acttattttg 10980 tgggtatcta tacttttatt agatttttaa tcaggctcct gatttctttt tatttcgatt 11040 gaattcctga acttgtatta ttcagtagat cgaataaatt ataaaaagat aaaatcataa 11100 aataatattt tatcctatca atcatattaa agcaatgaat atgtaaaatt aatcttatct 11160 ttattttaaa aaatcatata ggtttagtat ttttttaaaa ataaagatag gattagtttt 11220 actattcact gcttattact tttaaaaaaa tcataaaggt ttagtatttt tttaaaataa 11280 atataggaat agttttacta ttcactgctt taatagaaaa atagtttaaa atttaagata 11340 gttttaatcc cagcatttgc cacgtttgaa cgtgagccga aacgatgtcg ttacattatc 11400 ttaacctagc tgaaacgatg tcgtcataat atcgccaaat gccaactgga ctacgtcgaa 11460 cccacaaatc ccacaaagcg cgtgaaatca aatcgctcaa accacaaaaa agaacaacgc 11520 gtttgttaca cgctcaatcc cacgcgagta gagcacagta accttcaaat aagcgaatgg 11580 ggcataatca gaaatccgaa ataaacctag gggcattatc ggaaatgaaa agtagctcac 11640 tcaatataaa aatctaggaa ccctagtttt cgttatcact ctgtgctccc tcgctctatt 11700 tctcagtctc tgtgtttgcg gctgaggatt ccgaacgagt gaccttcttc gtttctcgca 11760 aaggtaacag cctctgctct tgtctcttcg attcgatcta tgcctgtctc ttatttacga 11820 tgatgtttct tcggttatgt ttttttattt atgctttatg ctgttgatgt tcggttgttt 11880 gtttcgcttt gtttttgtgg ttcagttttt taggattctt ttggtttttg aatcgattaa 11940 tcggaagaga ttttcgagtt atttggtgtg ttggaggtga atcttttttt tgaggtcata 12000 gatctgttgt atttgtgtta taaacatgcg actttgtatg attttttacg aggttatgat 12060 gttctggttg ttttattatg aatctgttga gacagaacca tgatttttgt tgatgttcgt 12120 ttacactatt aaaggtttgt tttaacagga ttaaaagttt tttaagcatg ttgaaggagt 12180 cttgtagata tgtaaccgtc gatagttttt ttgtgggttt gttcacatgt tatcaagctt 12240 aatcttttac tatgtatgcg accatatctg gatccagcaa aggcgatttt ttaattcctt 12300 gtgaaacttt tgtaatatga agttgaaatt ttgttattgg taaactataa atgtgtgaag 12360 ttggagtata cctttacctt cttatttggc tttgtgatag tttaatttat atgtattttg 12420 agttctgact tgtatttctt tgaattgatt ctagtttaag taatcatggc ctcctccgag 12480 aacgtcatca ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac 12540 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag 12600 ctgaaggtga cgaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc 12660 cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg 12720 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg 12780 accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc 12840 ggcgtgaact tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc 12900 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca caaggccctg 12960 aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag 13020 cccgtgcagc tgcccggcta ctactacgtg gacgccaagc tggacatcac ctcccacaac 13080 gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg 13140 tagttttgtg atctgatgat aagtggttgg ttcgtgtctc atgcacttgg gaggtgatct 13200 atttcacctg gtgtagtttg tgtttccgtc agttggaaaa acttatccct atcgatttcg 13260 ttttcatttt ctgcttttct tttatgtacc ttcgtttggg cttgtaacgg gcctttgtat 13320 ttcaactctc aataataatc caagtgcatg ttaaacaatt tgtcatctgt ttcggctttg 13380 atatactact ggtgaagatg ggccgtacta ctgcatcaca acgaaaaata ataataagat 13440 gaaaaacttg aagtggaaaa aaaaaaaaac ttgaatgttc actactactc attgaccata 13500 atgtttaaca tacatagctc aatagtattt ttgtgaatat ggcaacacaa acagtccaaa 13560 acaattgtct cttactatac caaaccaagg gcgccgcttg tttgccactc tttgtgtgca 13620 atagtgtgat taccacatct ccacattcaa tatattccct gaattatctg acgattttga 13680 tggctcactg ttttcccaag tcttgaattg tcttctgtgc gccagtcaaa tgcatatgtg 13740 ttgagtttat cttttaaata tcaagctttt gtttttaact tttgtttgta accaaaaact 13800 cacagtagga gtttgatcac ataattttat gtttgccttt gcaatttcta gtgagtcttt 13860 gattaaaagc ttgaaaagaa aatgcagcca agcttaccaa gtaagttatg tgtattaacc 13920 agaggaagag agaatcttgc aaaatttcaa caaacacaaa aagaagtatt actacgattg 13980 gtggagaaag aaaacgattc caaatcttga actgttgttg taaaagcata gcagaaagtg 14040 ggagacaacc gaaatagaaa tgactataac ttaatttaat gttatcatta taatttcttc 14100 tagcaaatat ttagaaagta aatatcacat caacctttaa tgtaattaag ctttctcttt 14160 ttgattcatg tgagatgaaa agaaaaaaaa gaagagaaaa gtgtagaaaa cacatcattt 14220 ctaagctgaa gctgaggtac aaattaatta ataagtgact agggtcacgt gaccctagtc 14280 acttatgatt ccgatgactt cgtaggttcc tagctcaagc cgctcgtgtc caagcgtcac 14340 ttacgattag ctaatgatta cggcatctag gaccgactag ttcttatgtg cttctagtct 14400 ccaaatgtgg ttgatagtta ttttgctcta agatcaacag taatgaagta taaatcatcg 14460 ttgtggtgtg ctactcggtt aattgagcat taacacacac aaacatgacg aggatggtat 14520 aatctccaaa aatgtgtact ttgttaggtg ggaccctata gccttgatta atgtgctatg 14580 ttaggcatgc ctggaaacgt gtgacgcata tgttttgtga acctgttgat attatatgtg 14640 cttttatatt accatatttt attaaaatac taatatttat tactagtaag atataacatt 14700 ctatctagct taaaaactaa ccataaatat tccataataa ctagatttac caaactaata 14760 tactaaatat acataataaa tacaaaatta acaagacaat aatcaatatt tatgagctta 14820 atatatttag acattatggt tggtcgacga taatcatgct aacttttcgt aattgcttga 14880 ttgaaatatg cttagaataa tgcctctttg ttctacatgg caaataggga ccattatggt 14940 gtaacaccct gggaaccaca aacaccccga aatgctacta aactacacaa ctaaccttca 15000 tatataaaat ttcgacagca tctcctttga aaatttgcat agacgtggaa gcaacagagt 15060 ataaacagat ctagctagct aagtttaaac ggccggccaa acgtgcaagc gctactagac 15120 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttatt 15180 tacaattgaa tatatcctgc ca 15202 <210> 163 <211> 15343 <212> DNA <213> Artificial Sequence <220> <223> RV026528 T-DNA <400> 163 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaacctcaac aagatcccaa taaccaacaa caacaacttc aacaacaaca acatgaagaa 1860 aacagcagtg gaagtagtag caaaagcagt tttctttgca ggcaaactag tacaaggtgg 1920 acacccacaa gtgatcagat aagaatcttg aaagaccttt actacaacaa tggagttagg 1980 tctccaagtg cagagcaaat acagagaatc tcagcaaggt taaggcagta cggtaagatt 2040 gaaggcaaga atgttttcta ttggtttcag aaccacaaag ctcgtgagag acaaaagaag 2100 agattcacaa atgatacaac aaccatgtcc atgcaaagag ctactactgc aactggtcat 2160 gccaaataca ttactacttc tcctgggatt tcatctccat cttctgctgg tgtacttggt 2220 gttggacaga tgggaagcaa catgggatat ggatctgttg ttatggagaa gagttttaga 2280 gactgttcaa tatctcctgc tgctggtggt ggtggtggtg gtggcagtaa tatgggtgga 2340 tatggatcca tgaacaacaa ttttggttgg attggtgcgg gtgtcgagcc atatccattt 2400 ttcaacaaga caaagcatat tcaaaaatat gaagaagaag atttctccca aactgagatt 2460 gaaacactac cactatttcc catgcatggt gaagacagta caaggtttgg ttcatctggc 2520 aacccaaacc cgaattggaa ctggtttcga acaggagatg aaatgattgg atctagaaca 2580 tcattggagc ttagcttgaa ttcttactct ggcgggtcaa ttaataactg aggtacctga 2640 catcttatag tctgcaacct ctcgtgtctg aattcctatc tttatcaagt gttattgctt 2700 ccacgactat aggacagctt tcgtcgaaag gttttgctca tgtgatctcg aaggattcat 2760 ctagtctgat ttttcgtgac ttgtatcggt tttattggat tcatccaaca tatatcaata 2820 aaaaatgagt tgtgtttcct ttcttcctag ttcagttaaa attatttccc tcctgcgctt 2880 gtgctgtaat tgtctgtgta cctgttgttt gtgactgtgt tagttccctt ggatatgatt 2940 tcgtatttga tatgtacatg gagatagctt agcttcatta ttggagtatg aagttagtat 3000 gacatagtca ctctcctgga aaattgacac tgcaaaccat atttttattc tgaaccacaa 3060 atcctagtca gtccgctggc atatgccgtc cgtttgctga atccagaacg tgggtttgga 3120 gatgtacggc tgagatgcct ctatgcgaag gggatttcgt ggtgaaacga gatgggagta 3180 gagcaacgcc cgtggaagat gcttcaaact tccacacttt tgagcaacga tcggcagtag 3240 taaggtagac gatttcaaga tcaaagcata tgaagataaa caacatcaac aacaaaattt 3300 gttggggttc tatagagaga aacagagcta catacataca ctgttttgta tctaccatct 3360 gagatgatga aaagatgaaa aactaaagaa tgccccggcg ccaacgccag gacacgccgc 3420 gcgcgcgtca cccgagccat ctcttgaccc agccggcgct gtatatttac acacgttgca 3480 gcatcgatca ccacctgttc gatcgcgtcg ccgtcaccaa gcttggcgcg ccggtcaccc 3540 ggtccgggcc tagaaggcca cccggggctc ggcgttaggc ttttagtccg ctaggtggct 3600 agaccgaggt taggacgcta gttcactagg ggcctagtgg tgttttaagg atgggcaagt 3660 ttgtacaaaa aagcaggctg gcgccggaac caattcagtc gagtgcggcc gccagtgtga 3720 tggatatctc gacccgggtg attgcggtta catcatgtac ggaaaaataa ttctaatcct 3780 tgatttaaat ttgaacttga ctatttattt attctttatt tcattttgta aatcatttta 3840 tgtatctcct ggcaagcaat tttatccacc ttgcaccaac accttcgggt tccataatca 3900 aaccacctta acttcacacc atgctgtaac tcacaccgcc cagcatctcc aatgtgaaag 3960 aagctaaaat ttaataaaca atcatacgaa gcagtgacaa aataccagat ggtattaatg 4020 cttcgataaa attaattgga aagtataaaa tggtagaaaa taataaatta taattaattt 4080 aagtaagata aaaaataatt aaaaactaaa atgttaaaat tttaaaaaaa ttattttaaa 4140 taatatttaa aaacattaaa aatcatttta aaaaatttat ttatagaaca attaaataaa 4200 tatttcagct aataaaaaac aaaagcttac ctagccttag aagacaactt gtccaacaat 4260 tagatgatac ccattgccct tacgttttct ttaacatcaa ttattgtttt tgtcaacaag 4320 ctatctttta gttttatttt attggtaaaa aatatgtcgc cttcaagttg catcatttaa 4380 cacatctcgt cattagaaaa ataaaactct tccctaaacg attagtagaa aaaatcattc 4440 gataataaat aagaaagaaa aattagaaaa aaataacttc attttaaaaa aatcattaag 4500 gctatatttt ttaaatgact aattttatat agactgtaac taaaagtata caatttatta 4560 tgctatgtat cttaaagaat tacttataaa aatctacgga agaatatctt acaaagtgaa 4620 aaacaaatga gaaagaattt agtgggatga ttatgatttt atttgaaaat tgaaaaaata 4680 attattaaag actttagtgg agtaagaaag ctttcctatt agtcttttct tatccataaa 4740 aaaaaaaaaa aaaatctagc gtgacagctt ttccatagat tttaataatg taaaatactg 4800 gtagcagccg accgttcagg taatggacac tgtggtccta acttgcaacg ggtgcgggcc 4860 caatttaata acgccgtggt aacggataaa gccaagcgtg aagcggtgaa ggtacatctc 4920 tgactccgtc aagattacga aaccgtcaac tacgaaggac tccccgaaat atcatctgtg 4980 tcataaacac caagtcacac catacatggg cacgcgtcac aatatgattg gagaacggtt 5040 ccaccgcata tgctataaaa tgcccccaca cccctcgacc ctaatcgcac ttcaattgca 5100 atcaaattag ttcattctct ttgcgcagtt ccctacctct cctttcaagg ttcgtagatt 5160 tcttccgttt ttttttcttc ttctttattg tttgttctac atcagcatga tgttgatttg 5220 attgtgtttt ctatcgtttc atcgattata aattttcata atcagaagat tcagctttta 5280 ttaatgcaag aacgtcctta attgatgatt ttataaccgt aaattaggtc taattagagt 5340 ttttttcata aagattttca gatccgttta caacaagcct taattgttga ttctgtagtc 5400 gtagattaag gtttttttca tgaactactt cagatccgtt aaacaacagc cttatttgtt 5460 gatacttcag tcgtttttca agaaattgtt cagatccgtt gataaaagcc ttattcgttg 5520 attctgtatg gtatttcaag agatattgct caggtccttt agcaactacc ttatttgttg 5580 attctgtggc catagattag gatttttttt cacgaaattg cttcttgaaa ttacgtgatg 5640 gattttgatt ctgatttatc ttgtgattgt tgactctaca gccatggccc acagcaagca 5700 cggcctgaag gaggagatga ccatgaagta ccacatggag ggctgcgtga acggccacaa 5760 gttcgtgatc accggcgagg gcatcggcta ccccttcaag ggcaagcaga ccatcaacct 5820 gtgcgtgatc gagggcggcc ccctgccctt cagcgaggac atcctgagcg ccggcttcaa 5880 gtacggcgac cggatcttca ccgagtaccc ccaggacatc gtggactact tcaagaacag 5940 ctgccccgcc ggctacacct ggggccggag cttcctgttc gaggacggcg ccgtgtgcat 6000 ctgtaacgtg gacatcaccg tgagcgtgaa ggagaactgc atctaccaca agagcatctt 6060 caacggcgtg aacttccccg ccgacggccc cgtgatgaag aagatgacca ccaactggga 6120 ggccagctgc gagaagatca tgcccgtgcc taagcagggc atcctgaagg gcgacgtgag 6180 catgtacctg ctgctgaagg acggcggccg gtaccggtgc cagttcgaca ccgtgtacaa 6240 ggccaagagc gtgcccagca agatgcccga gtggcacttc atccagcaca agctgctgcg 6300 ggaggaccgg agcgacgcca agaaccagaa gtggcagctg accgagcacg ccatcgcctt 6360 ccccagcgcc ctggcctgag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa 6420 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga 6480 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt 6540 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg 6600 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattctagt 6660 ggccggccca gctgatatct agacccagct ttcttgtaca aagtggccgt taacggatcg 6720 gccagaatgg cccggaccgg gttaccgaat tcgagctcgg taccctggga tccgatatcg 6780 atgggccctg gccgaagctt ggtcacccgg tccgggccta gaaggccagc ttcggccgcc 6840 ccgggcaact ttattataca aagttgatgg tacggatccg tcgacggcgc gccgcggacc 6900 tgaattccag cacactggcg gccgttagtc taaaaacggc ccctaactaa cgcttagttg 6960 cccgctagat tagtcccccg ttagtgccta gttagacaca cgacgaacta ggggctacta 7020 gtaacggccg ccagtgtgct cggtacggcc agaatggcca tctggattca gcagggggtg 7080 attgcggtta catcatgtac ggaaaaataa ttctaatcct tgatttaaat ttgaacttga 7140 ctatttattt attctttatt tcattttgta aatcatttta tgtatctcct ggcaagcaat 7200 tttatccacc ttgcaccaac accttcgggt tccataatca aaccacctta acttcacacc 7260 atgctgtaac tcacaccgcc cagcatctcc aatgtgaaag aagctaaaat ttaataaaca 7320 atcatacgaa gcagtgacaa aataccagat ggtattaatg cttcgataaa attaattgga 7380 aagtataaaa tggtagaaaa taataaatta taattaattt aagtaagata aaaaataatt 7440 aaaaactaaa atgttaaaat tttaaaaaaa ttattttaaa taatatttaa aaacattaaa 7500 aatcatttta aaaaatttat ttatagaaca attaaataaa tatttcagct aataaaaaac 7560 aaaagcttac ctagccttag aagacaactt gtccaacaat tagatgatac ccattgccct 7620 tacgttttct ttaacatcaa ttattgtttt tgtcaacaag ctatctttta gttttatttt 7680 attggtaaaa aatatgtcgc cttcaagttg catcatttaa cacatctcgt cattagaaaa 7740 ataaaactct tccctaaacg attagtagaa aaaatcattc gataataaat aagaaagaaa 7800 aattagaaaa aaataacttc attttaaaaa aatcattaag gctatatttt ttaaatgact 7860 aattttatat agactgtaac taaaagtata caatttatta tgctatgtat cttaaagaat 7920 tacttataaa aatctacgga agaatatctt acaaagtgaa aaacaaatga gaaagaattt 7980 agtgggatga ttatgatttt atttgaaaat tgaaaaaata attattaaag actttagtgg 8040 agtaagaaag ctttcctatt agtcttttct tatccataaa aaaaaaaaaa aaaatctagc 8100 gtgacagctt ttccatagat tttaataatg taaaatactg gtagcagccg accgttcagg 8160 taatggacac tgtggtccta acttgcaacg ggtgcgggcc caatttaata acgccgtggt 8220 aacggataaa gccaagcgtg aagcggtgaa ggtacatctc tgactccgtc aagattacga 8280 aaccgtcaac tacgaaggac tccccgaaat atcatctgtg tcataaacac caagtcacac 8340 catacatggg cacgcgtcac aatatgattg gagaacggtt ccaccgcata tgctataaaa 8400 tgcccccaca cccctcgacc ctaatcgcac ttcaattgca atcaaattag ttcattctct 8460 ttgcgcagtt ccctacctct cctttcaagg ttcgtagatt tcttccgttt ttttttcttc 8520 ttctttattg tttgttctac atcagcatga tgttgatttg attgtgtttt ctatcgtttc 8580 atcgattata aattttcata atcagaagat tcagctttta ttaatgcaag aacgtcctta 8640 attgatgatt ttataaccgt aaattaggtc taattagagt ttttttcata aagattttca 8700 gatccgttta caacaagcct taattgttga ttctgtagtc gtagattaag gtttttttca 8760 tgaactactt cagatccgtt aaacaacagc cttatttgtt gatacttcag tcgtttttca 8820 agaaattgtt cagatccgtt gataaaagcc ttattcgttg attctgtatg gtatttcaag 8880 agatattgct caggtccttt agcaactacc ttatttgttg attctgtggc catagattag 8940 gatttttttt cacgaaattg cttcttgaaa ttacgtgatg gattttgatt ctgatttatc 9000 ttgtgattgt tgactctaca gccatggctg caactactct tacatctgct ttacctggtg 9060 ctttcagttc atcccagaga ccaagtgctc ctttcaactt acaaagatca cctagagtgc 9120 ttagaaggtt caacagaaag acaggacgtc aacctcgtgg acttgttcgt gctgcaaagg 9180 ctcaagcggc gcaaaacctg ccagagaaat tcgatgaaac aagactctgg gaggccctca 9240 ggggatttgg ggttagtcca agtagggtta cttacgctcc cgtcggattc ggagattacc 9300 actggaccgt cacagatgaa gacgggagac cttggttcgc aactgtcagt gacctcgaac 9360 acaaagagca ttgcgggcag ggagcacaag cagcccttaa aggactcaga caagctatgg 9420 acactgccct cgcacttaga gatagggacg gactcagatt cgtggttgct ccagtggcag 9480 caacagatgg gggtggccct gtccttcccc ttgacgctag gtatgcactc acagtcttcc 9540 ctcacgtccc aggaagaaca ggagagttcg gacaaagact cacagaggca gagagggaca 9600 gactgcttgc tcttctggca gaactccacg gaagaacacc tcctgaaaca accccaccag 9660 ccgatatgga accaccagga cttcctggtg ttagagcagc actcgccgaa agtgaaggac 9720 cttggagtgg aggaccattt gcagaacccg ctagacttct tctcagagaa cacgaggcca 9780 cactgcacgc aagactcgct gaattcgaag ccctcgtcgc aagagttaag ggtcgtggag 9840 cacctcttgt cgttacccac ggagaaccac acccaggcaa cttgatcctc ggagaggaag 9900 gctatctcct cgtggactgg gatacagttg gactggctcc agctgaaagg gatttgagtc 9960 tcatctcaga cgacccagct gacctcgcaa gatacgcaga gcttacagga agaactccag 10020 acccagacgc actcgcactc tatagactgc gttggagtct ccttgatgtg gctgagttcg 10080 tcgagtggtt cagggctgaa caccaaagaa cccaagacac cgagagtgca tggaaggggt 10140 tcactgacac acttgggcaa ttggctgctg gtggatcagc tgcatagcaa acttatctct 10200 gttatgaatc agaagaagtt catgtctcgt ttcatttaaa actttggtgg tttgtgtttt 10260 ggggccttgt aaagcccctg atgaataatt gttcaactat gtttccgttc ctgtgttata 10320 cctttctttc taatgagtaa tgacatcaaa cttcttctgt attgaaatta tgtccttgtg 10380 agtctcttta tcatcgtttc gtctttacat tatatgtgct acttttgtct aatgagcctg 10440 aaaagtggct ccaatggtac gcactggaag atttgttggc ttctggtaga tatagcgaca 10500 gtgttgagct tgtaatatca tgtctcttat tgctaaatta gttcctttct taacagaaac 10560 cttcaaagtt tttgtttttg ttttcattta cctaatgtac acatacgctg gccatgacta 10620 acaacatgtc caggcttaga gcatattttt ttctagctta aattgttaac ttgtcattca 10680 gtaaaatccg agaattgtga agctctaatt gaagctaatt cgttttataa agtcagttaa 10740 aaagtatact aaattatcca acttttcttc aaaatctcaa aattctatga caaaacgata 10800 gtctttgttt atgtcagtac cacaaagagg tggaaaaaaa caccaaaaaa acaataagca 10860 aactatacac tgagaagaaa aataaaagag agctcaatag atgttttata ctaacggtag 10920 attagatcaa agatccaagc tttactctac atagagcaga acccagaatc ccttcatatc 10980 tcttttattc tagcaccgat aatctactga aaagaagaca cttagagctc tgtctctttg 11040 tcaaagaagt cccagccgtc atccagaagc tccttacgtt cattaacaga ggccgcaatt 11100 ggatcgggtt tacttatttt gtgggtatct atacttttat tagattttta atcaggctcc 11160 tgatttcttt ttatttcgat tgaattcctg aacttgtatt attcagtaga tcgaataaat 11220 tataaaaaga taaaatcata aaataatatt ttatcctatc aatcatatta aagcaatgaa 11280 tatgtaaaat taatcttatc tttattttaa aaaatcatat aggtttagta tttttttaaa 11340 aataaagata ggattagttt tactattcac tgcttattac ttttaaaaaa atcataaagg 11400 tttagtattt ttttaaaata aatataggaa tagttttact attcactgct ttaatagaaa 11460 aatagtttaa aatttaagat agttttaatc ccagcatttg ccacgtttga acgtgagccg 11520 aaacgatgtc gttacattat cttaacctag ctgaaacgat gtcgtcataa tatcgccaaa 11580 tgccaactgg actacgtcga acccacaaat cccacaaagc gcgtgaaatc aaatcgctca 11640 aaccacaaaa aagaacaacg cgtttgttac acgctcaatc ccacgcgagt agagcacagt 11700 aaccttcaaa taagcgaatg gggcataatc agaaatccga aataaaccta ggggcattat 11760 cggaaatgaa aagtagctca ctcaatataa aaatctagga accctagttt tcgttatcac 11820 tctgtgctcc ctcgctctat ttctcagtct ctgtgtttgc ggctgaggat tccgaacgag 11880 tgaccttctt cgtttctcgc aaaggtaaca gcctctgctc ttgtctcttc gattcgatct 11940 atgcctgtct cttatttacg atgatgtttc ttcggttatg tttttttatt tatgctttat 12000 gctgttgatg ttcggttgtt tgtttcgctt tgtttttgtg gttcagtttt ttaggattct 12060 tttggttttt gaatcgatta atcggaagag attttcgagt tatttggtgt gttggaggtg 12120 aatctttttt ttgaggtcat agatctgttg tatttgtgtt ataaacatgc gactttgtat 12180 gattttttac gaggttatga tgttctggtt gttttattat gaatctgttg agacagaacc 12240 atgatttttg ttgatgttcg tttacactat taaaggtttg ttttaacagg attaaaagtt 12300 ttttaagcat gttgaaggag tcttgtagat atgtaaccgt cgatagtttt tttgtgggtt 12360 tgttcacatg ttatcaagct taatctttta ctatgtatgc gaccatatct ggatccagca 12420 aaggcgattt tttaattcct tgtgaaactt ttgtaatatg aagttgaaat tttgttattg 12480 gtaaactata aatgtgtgaa gttggagtat acctttacct tcttatttgg ctttgtgata 12540 gtttaattta tatgtatttt gagttctgac ttgtatttct ttgaattgat tctagtttaa 12600 gtaatcatgg cctcctccga gaacgtcatc accgagttca tgcgcttcaa ggtgcgcatg 12660 gagggcaccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 12720 gagggccaca acaccgtgaa gctgaaggtg acgaagggcg gccccctgcc cttcgcctgg 12780 gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac 12840 atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 12900 ttcgaggacg gcggcgtggc gaccgtgacc caggactcct ccctgcagga cggctgcttc 12960 atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt gatgcagaag 13020 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag 13080 ggcgagaccc acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag 13140 tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacgccaag 13200 ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag 13260 ggccgccacc acctgttcct gtagttttgt gatctgatga taagtggttg gttcgtgtct 13320 catgcacttg ggaggtgatc tatttcacct ggtgtagttt gtgtttccgt cagttggaaa 13380 aacttatccc tatcgatttc gttttcattt tctgcttttc ttttatgtac cttcgtttgg 13440 gcttgtaacg ggcctttgta tttcaactct caataataat ccaagtgcat gttaaacaat 13500 ttgtcatctg tttcggcttt gatatactac tggtgaagat gggccgtact actgcatcac 13560 aacgaaaaat aataataaga tgaaaaactt gaagtggaaa aaaaaaaaaa cttgaatgtt 13620 cactactact cattgaccat aatgtttaac atacatagct caatagtatt tttgtgaata 13680 tggcaacaca aacagtccaa aacaattgtc tcttactata ccaaaccaag ggcgccgctt 13740 gtttgccact ctttgtgtgc aatagtgtga ttaccacatc tccacattca atatattccc 13800 tgaattatct gacgattttg atggctcact gttttcccaa gtcttgaatt gtcttctgtg 13860 cgccagtcaa atgcatatgt gttgagttta tcttttaaat atcaagcttt tgtttttaac 13920 ttttgtttgt aaccaaaaac tcacagtagg agtttgatca cataatttta tgtttgcctt 13980 tgcaatttct agtgagtctt tgattaaaag cttgaaaaga aaatgcagcc aagcttacca 14040 agtaagttat gtgtattaac cagaggaaga gagaatcttg caaaatttca acaaacacaa 14100 aaagaagtat tactacgatt ggtggagaaa gaaaacgatt ccaaatcttg aactgttgtt 14160 gtaaaagcat agcagaaagt gggagacaac cgaaatagaa atgactataa cttaatttaa 14220 tgttatcatt ataatttctt ctagcaaata tttagaaagt aaatatcaca tcaaccttta 14280 atgtaattaa gctttctctt tttgattcat gtgagatgaa aagaaaaaaa agaagagaaa 14340 agtgtagaaa acacatcatt tctaagctga agctgaggta caaattaatt aataagtgac 14400 tagggtcacg tgaccctagt cacttatgat tccgatgact tcgtaggttc ctagctcaag 14460 ccgctcgtgt ccaagcgtca cttacgatta gctaatgatt acggcatcta ggaccgacta 14520 gttcttatgt gcttctagtc tccaaatgtg gttgatagtt attttgctct aagatcaaca 14580 gtaatgaagt ataaatcatc gttgtggtgt gctactcggt taattgagca ttaacacaca 14640 caaacatgac gaggatggta taatctccaa aaatgtgtac tttgttaggt gggaccctat 14700 agccttgatt aatgtgctat gttaggcatg cctggaaacg tgtgacgcat atgttttgtg 14760 aacctgttga tattatatgt gcttttatat taccatattt tattaaaata ctaatattta 14820 ttactagtaa gatataacat tctatctagc ttaaaaacta accataaata ttccataata 14880 actagattta ccaaactaat atactaaata tacataataa atacaaaatt aacaagacaa 14940 taatcaatat ttatgagctt aatatattta gacattatgg ttggtcgacg ataatcatgc 15000 taacttttcg taattgcttg attgaaatat gcttagaata atgcctcttt gttctacatg 15060 gcaaataggg accattatgg tgtaacaccc tgggaaccac aaacaccccg aaatgctact 15120 aaactacaca actaaccttc atatataaaa tttcgacagc atctcctttg aaaatttgca 15180 tagacgtgga agcaacagag tataaacaga tctagctagc taagtttaaa cggccggcca 15240 aacgtgcaag cgctactaga caattcagta cattaaaaac gtccgcaatg tgttattaag 15300 ttgtctaagc gtcaatttat ttacaattga atatatcctg cca 15343 <210> 164 <211> 15303 <212> DNA <213> Artificial Sequence <220> <223> RV026520 T-DNA <400> 164 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaacctcacc aacaacaacc aaatgaggat aataacggtg gcgccaaagg gaactttctt 1860 tgcaggcaaa ccagtacaag gtggaatccc actactgacc agataagaat attgaaggaa 1920 ctgtactaca tcaaaggtgt taggtcccca aatggagctg agattcagca gatctctgct 1980 aggcttagaa agtacggcaa gattgaaggc aagaatgtat tttattggtt tcagaaccat 2040 aaagctcgtg agaggcaaaa gaaaagactc accaatgaag tccccatgca acaaagaact 2100 gcctggaaac ctgaagatta ttactcttac aagtactcca acagtaataa taatcctggg 2160 ttttcttcgg cttcttcatc tgcaaatacc ggtgtggtta ctgttgggca gacagatagt 2220 catggatatg gatcggtaac catgcaggag aagaattctt gggactgttc agcaccagct 2280 ggtggtagca atggtgctgg tagtggatcc atgagcaata ttaactatgg atcaggggtt 2340 gacattaact ctcacagttc atcctatgct gtatttggtc aagaacaaga agcagctgca 2400 aagattgaga ctcttcctct atttccaatg cttggtgagg acatcagtag ctctttcaac 2460 atcaacaata tcaacccaga cttttattac agtagcggct gtggctatgg tgattatggc 2520 aatgacactt cttcccgtac ttccctggac cttagtctct actcctacaa tggccaacca 2580 caagattatt aggtacctga catcttatag tctgcaacct ctcgtgtctg aattcctatc 2640 tttatcaagt gttattgctt ccacgactat aggacagctt tcgtcgaaag gttttgctca 2700 tgtgatctcg aaggattcat ctagtctgat ttttcgtgac ttgtatcggt tttattggat 2760 tcatccaaca tatatcaata aaaaatgagt tgtgtttcct ttcttcctag ttcagttaaa 2820 attatttccc tcctgcgctt gtgctgtaat tgtctgtgta cctgttgttt gtgactgtgt 2880 tagttccctt ggatatgatt tcgtatttga tatgtacatg gagatagctt agcttcatta 2940 ttggagtatg aagttagtat gacatagtca ctctcctgga aaattgacac tgcaaaccat 3000 atttttattc tgaaccacaa atcctagtca gtccgctggc atatgccgtc cgtttgctga 3060 atccagaacg tgggtttgga gatgtacggc tgagatgcct ctatgcgaag gggatttcgt 3120 ggtgaaacga gatgggagta gagcaacgcc cgtggaagat gcttcaaact tccacacttt 3180 tgagcaacga tcggcagtag taaggtagac gatttcaaga tcaaagcata tgaagataaa 3240 caacatcaac aacaaaattt gttggggttc tatagagaga aacagagcta catacataca 3300 ctgttttgta tctaccatct gagatgatga aaagatgaaa aactaaagaa tgccccggcg 3360 ccaacgccag gacacgccgc gcgcgcgtca cccgagccat ctcttgaccc agccggcgct 3420 gtatatttac acacgttgca gcatcgatca ccacctgttc gatcgcgtcg ccgtcaccaa 3480 gcttggcgcg ccggtcaccc ggtccgggcc tagaaggcca cccggggctc ggcgttaggc 3540 ttttagtccg ctaggtggct agaccgaggt taggacgcta gttcactagg ggcctagtgg 3600 tgttttaagg atgggcaagt ttgtacaaaa aagcaggctg gcgccggaac caattcagtc 3660 gagtgcggcc gccagtgtga tggatatctc gacccgggtg attgcggtta catcatgtac 3720 ggaaaaataa ttctaatcct tgatttaaat ttgaacttga ctatttattt attctttatt 3780 tcattttgta aatcatttta tgtatctcct ggcaagcaat tttatccacc ttgcaccaac 3840 accttcgggt tccataatca aaccacctta acttcacacc atgctgtaac tcacaccgcc 3900 cagcatctcc aatgtgaaag aagctaaaat ttaataaaca atcatacgaa gcagtgacaa 3960 aataccagat ggtattaatg cttcgataaa attaattgga aagtataaaa tggtagaaaa 4020 taataaatta taattaattt aagtaagata aaaaataatt aaaaactaaa atgttaaaat 4080 tttaaaaaaa ttattttaaa taatatttaa aaacattaaa aatcatttta aaaaatttat 4140 ttatagaaca attaaataaa tatttcagct aataaaaaac aaaagcttac ctagccttag 4200 aagacaactt gtccaacaat tagatgatac ccattgccct tacgttttct ttaacatcaa 4260 ttattgtttt tgtcaacaag ctatctttta gttttatttt attggtaaaa aatatgtcgc 4320 cttcaagttg catcatttaa cacatctcgt cattagaaaa ataaaactct tccctaaacg 4380 attagtagaa aaaatcattc gataataaat aagaaagaaa aattagaaaa aaataacttc 4440 attttaaaaa aatcattaag gctatatttt ttaaatgact aattttatat agactgtaac 4500 taaaagtata caatttatta tgctatgtat cttaaagaat tacttataaa aatctacgga 4560 agaatatctt acaaagtgaa aaacaaatga gaaagaattt agtgggatga ttatgatttt 4620 atttgaaaat tgaaaaaata attattaaag actttagtgg agtaagaaag ctttcctatt 4680 agtcttttct tatccataaa aaaaaaaaaa aaaatctagc gtgacagctt ttccatagat 4740 tttaataatg taaaatactg gtagcagccg accgttcagg taatggacac tgtggtccta 4800 acttgcaacg ggtgcgggcc caatttaata acgccgtggt aacggataaa gccaagcgtg 4860 aagcggtgaa ggtacatctc tgactccgtc aagattacga aaccgtcaac tacgaaggac 4920 tccccgaaat atcatctgtg tcataaacac caagtcacac catacatggg cacgcgtcac 4980 aatatgattg gagaacggtt ccaccgcata tgctataaaa tgcccccaca cccctcgacc 5040 ctaatcgcac ttcaattgca atcaaattag ttcattctct ttgcgcagtt ccctacctct 5100 cctttcaagg ttcgtagatt tcttccgttt ttttttcttc ttctttattg tttgttctac 5160 atcagcatga tgttgatttg attgtgtttt ctatcgtttc atcgattata aattttcata 5220 atcagaagat tcagctttta ttaatgcaag aacgtcctta attgatgatt ttataaccgt 5280 aaattaggtc taattagagt ttttttcata aagattttca gatccgttta caacaagcct 5340 taattgttga ttctgtagtc gtagattaag gtttttttca tgaactactt cagatccgtt 5400 aaacaacagc cttatttgtt gatacttcag tcgtttttca agaaattgtt cagatccgtt 5460 gataaaagcc ttattcgttg attctgtatg gtatttcaag agatattgct caggtccttt 5520 agcaactacc ttatttgttg attctgtggc catagattag gatttttttt cacgaaattg 5580 cttcttgaaa ttacgtgatg gattttgatt ctgatttatc ttgtgattgt tgactctaca 5640 gccatggccc acagcaagca cggcctgaag gaggagatga ccatgaagta ccacatggag 5700 ggctgcgtga acggccacaa gttcgtgatc accggcgagg gcatcggcta ccccttcaag 5760 ggcaagcaga ccatcaacct gtgcgtgatc gagggcggcc ccctgccctt cagcgaggac 5820 atcctgagcg ccggcttcaa gtacggcgac cggatcttca ccgagtaccc ccaggacatc 5880 gtggactact tcaagaacag ctgccccgcc ggctacacct ggggccggag cttcctgttc 5940 gaggacggcg ccgtgtgcat ctgtaacgtg gacatcaccg tgagcgtgaa ggagaactgc 6000 atctaccaca agagcatctt caacggcgtg aacttccccg ccgacggccc cgtgatgaag 6060 aagatgacca ccaactggga ggccagctgc gagaagatca tgcccgtgcc taagcagggc 6120 atcctgaagg gcgacgtgag catgtacctg ctgctgaagg acggcggccg gtaccggtgc 6180 cagttcgaca ccgtgtacaa ggccaagagc gtgcccagca agatgcccga gtggcacttc 6240 atccagcaca agctgctgcg ggaggaccgg agcgacgcca agaaccagaa gtggcagctg 6300 accgagcacg ccatcgcctt ccccagcgcc ctggcctgag agctcgaatt tccccgatcg 6360 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat 6420 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac 6480 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat 6540 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt 6600 actagatcgg gaattctagt ggccggccca gctgatatct agacccagct ttcttgtaca 6660 aagtggccgt taacggatcg gccagaatgg cccggaccgg gttaccgaat tcgagctcgg 6720 taccctggga tccgatatcg atgggccctg gccgaagctt ggtcacccgg tccgggccta 6780 gaaggccagc ttcggccgcc ccgggcaact ttattataca aagttgatgg tacggatccg 6840 tcgacggcgc gccgcggacc tgaattccag cacactggcg gccgttagtc taaaaacggc 6900 ccctaactaa cgcttagttg cccgctagat tagtcccccg ttagtgccta gttagacaca 6960 cgacgaacta ggggctacta gtaacggccg ccagtgtgct cggtacggcc agaatggcca 7020 tctggattca gcagggggtg attgcggtta catcatgtac ggaaaaataa ttctaatcct 7080 tgatttaaat ttgaacttga ctatttattt attctttatt tcattttgta aatcatttta 7140 tgtatctcct ggcaagcaat tttatccacc ttgcaccaac accttcgggt tccataatca 7200 aaccacctta acttcacacc atgctgtaac tcacaccgcc cagcatctcc aatgtgaaag 7260 aagctaaaat ttaataaaca atcatacgaa gcagtgacaa aataccagat ggtattaatg 7320 cttcgataaa attaattgga aagtataaaa tggtagaaaa taataaatta taattaattt 7380 aagtaagata aaaaataatt aaaaactaaa atgttaaaat tttaaaaaaa ttattttaaa 7440 taatatttaa aaacattaaa aatcatttta aaaaatttat ttatagaaca attaaataaa 7500 tatttcagct aataaaaaac aaaagcttac ctagccttag aagacaactt gtccaacaat 7560 tagatgatac ccattgccct tacgttttct ttaacatcaa ttattgtttt tgtcaacaag 7620 ctatctttta gttttatttt attggtaaaa aatatgtcgc cttcaagttg catcatttaa 7680 cacatctcgt cattagaaaa ataaaactct tccctaaacg attagtagaa aaaatcattc 7740 gataataaat aagaaagaaa aattagaaaa aaataacttc attttaaaaa aatcattaag 7800 gctatatttt ttaaatgact aattttatat agactgtaac taaaagtata caatttatta 7860 tgctatgtat cttaaagaat tacttataaa aatctacgga agaatatctt acaaagtgaa 7920 aaacaaatga gaaagaattt agtgggatga ttatgatttt atttgaaaat tgaaaaaata 7980 attattaaag actttagtgg agtaagaaag ctttcctatt agtcttttct tatccataaa 8040 aaaaaaaaaa aaaatctagc gtgacagctt ttccatagat tttaataatg taaaatactg 8100 gtagcagccg accgttcagg taatggacac tgtggtccta acttgcaacg ggtgcgggcc 8160 caatttaata acgccgtggt aacggataaa gccaagcgtg aagcggtgaa ggtacatctc 8220 tgactccgtc aagattacga aaccgtcaac tacgaaggac tccccgaaat atcatctgtg 8280 tcataaacac caagtcacac catacatggg cacgcgtcac aatatgattg gagaacggtt 8340 ccaccgcata tgctataaaa tgcccccaca cccctcgacc ctaatcgcac ttcaattgca 8400 atcaaattag ttcattctct ttgcgcagtt ccctacctct cctttcaagg ttcgtagatt 8460 tcttccgttt ttttttcttc ttctttattg tttgttctac atcagcatga tgttgatttg 8520 attgtgtttt ctatcgtttc atcgattata aattttcata atcagaagat tcagctttta 8580 ttaatgcaag aacgtcctta attgatgatt ttataaccgt aaattaggtc taattagagt 8640 ttttttcata aagattttca gatccgttta caacaagcct taattgttga ttctgtagtc 8700 gtagattaag gtttttttca tgaactactt cagatccgtt aaacaacagc cttatttgtt 8760 gatacttcag tcgtttttca agaaattgtt cagatccgtt gataaaagcc ttattcgttg 8820 attctgtatg gtatttcaag agatattgct caggtccttt agcaactacc ttatttgttg 8880 attctgtggc catagattag gatttttttt cacgaaattg cttcttgaaa ttacgtgatg 8940 gattttgatt ctgatttatc ttgtgattgt tgactctaca gccatggctg caactactct 9000 tacatctgct ttacctggtg ctttcagttc atcccagaga ccaagtgctc ctttcaactt 9060 acaaagatca cctagagtgc ttagaaggtt caacagaaag acaggacgtc aacctcgtgg 9120 acttgttcgt gctgcaaagg ctcaagcggc gcaaaacctg ccagagaaat tcgatgaaac 9180 aagactctgg gaggccctca ggggatttgg ggttagtcca agtagggtta cttacgctcc 9240 cgtcggattc ggagattacc actggaccgt cacagatgaa gacgggagac cttggttcgc 9300 aactgtcagt gacctcgaac acaaagagca ttgcgggcag ggagcacaag cagcccttaa 9360 aggactcaga caagctatgg acactgccct cgcacttaga gatagggacg gactcagatt 9420 cgtggttgct ccagtggcag caacagatgg gggtggccct gtccttcccc ttgacgctag 9480 gtatgcactc acagtcttcc ctcacgtccc aggaagaaca ggagagttcg gacaaagact 9540 cacagaggca gagagggaca gactgcttgc tcttctggca gaactccacg gaagaacacc 9600 tcctgaaaca accccaccag ccgatatgga accaccagga cttcctggtg ttagagcagc 9660 actcgccgaa agtgaaggac cttggagtgg aggaccattt gcagaacccg ctagacttct 9720 tctcagagaa cacgaggcca cactgcacgc aagactcgct gaattcgaag ccctcgtcgc 9780 aagagttaag ggtcgtggag cacctcttgt cgttacccac ggagaaccac acccaggcaa 9840 cttgatcctc ggagaggaag gctatctcct cgtggactgg gatacagttg gactggctcc 9900 agctgaaagg gatttgagtc tcatctcaga cgacccagct gacctcgcaa gatacgcaga 9960 gcttacagga agaactccag acccagacgc actcgcactc tatagactgc gttggagtct 10020 ccttgatgtg gctgagttcg tcgagtggtt cagggctgaa caccaaagaa cccaagacac 10080 cgagagtgca tggaaggggt tcactgacac acttgggcaa ttggctgctg gtggatcagc 10140 tgcatagcaa acttatctct gttatgaatc agaagaagtt catgtctcgt ttcatttaaa 10200 actttggtgg tttgtgtttt ggggccttgt aaagcccctg atgaataatt gttcaactat 10260 gtttccgttc ctgtgttata cctttctttc taatgagtaa tgacatcaaa cttcttctgt 10320 attgaaatta tgtccttgtg agtctcttta tcatcgtttc gtctttacat tatatgtgct 10380 acttttgtct aatgagcctg aaaagtggct ccaatggtac gcactggaag atttgttggc 10440 ttctggtaga tatagcgaca gtgttgagct tgtaatatca tgtctcttat tgctaaatta 10500 gttcctttct taacagaaac cttcaaagtt tttgtttttg ttttcattta cctaatgtac 10560 acatacgctg gccatgacta acaacatgtc caggcttaga gcatattttt ttctagctta 10620 aattgttaac ttgtcattca gtaaaatccg agaattgtga agctctaatt gaagctaatt 10680 cgttttataa agtcagttaa aaagtatact aaattatcca acttttcttc aaaatctcaa 10740 aattctatga caaaacgata gtctttgttt atgtcagtac cacaaagagg tggaaaaaaa 10800 caccaaaaaa acaataagca aactatacac tgagaagaaa aataaaagag agctcaatag 10860 atgttttata ctaacggtag attagatcaa agatccaagc tttactctac atagagcaga 10920 acccagaatc ccttcatatc tcttttattc tagcaccgat aatctactga aaagaagaca 10980 cttagagctc tgtctctttg tcaaagaagt cccagccgtc atccagaagc tccttacgtt 11040 cattaacaga ggccgcaatt ggatcgggtt tacttatttt gtgggtatct atacttttat 11100 tagattttta atcaggctcc tgatttcttt ttatttcgat tgaattcctg aacttgtatt 11160 attcagtaga tcgaataaat tataaaaaga taaaatcata aaataatatt ttatcctatc 11220 aatcatatta aagcaatgaa tatgtaaaat taatcttatc tttattttaa aaaatcatat 11280 aggtttagta tttttttaaa aataaagata ggattagttt tactattcac tgcttattac 11340 ttttaaaaaa atcataaagg tttagtattt ttttaaaata aatataggaa tagttttact 11400 attcactgct ttaatagaaa aatagtttaa aatttaagat agttttaatc ccagcatttg 11460 ccacgtttga acgtgagccg aaacgatgtc gttacattat cttaacctag ctgaaacgat 11520 gtcgtcataa tatcgccaaa tgccaactgg actacgtcga acccacaaat cccacaaagc 11580 gcgtgaaatc aaatcgctca aaccacaaaa aagaacaacg cgtttgttac acgctcaatc 11640 ccacgcgagt agagcacagt aaccttcaaa taagcgaatg gggcataatc agaaatccga 11700 aataaaccta ggggcattat cggaaatgaa aagtagctca ctcaatataa aaatctagga 11760 accctagttt tcgttatcac tctgtgctcc ctcgctctat ttctcagtct ctgtgtttgc 11820 ggctgaggat tccgaacgag tgaccttctt cgtttctcgc aaaggtaaca gcctctgctc 11880 ttgtctcttc gattcgatct atgcctgtct cttatttacg atgatgtttc ttcggttatg 11940 tttttttatt tatgctttat gctgttgatg ttcggttgtt tgtttcgctt tgtttttgtg 12000 gttcagtttt ttaggattct tttggttttt gaatcgatta atcggaagag attttcgagt 12060 tatttggtgt gttggaggtg aatctttttt ttgaggtcat agatctgttg tatttgtgtt 12120 ataaacatgc gactttgtat gattttttac gaggttatga tgttctggtt gttttattat 12180 gaatctgttg agacagaacc atgatttttg ttgatgttcg tttacactat taaaggtttg 12240 ttttaacagg attaaaagtt ttttaagcat gttgaaggag tcttgtagat atgtaaccgt 12300 cgatagtttt tttgtgggtt tgttcacatg ttatcaagct taatctttta ctatgtatgc 12360 gaccatatct ggatccagca aaggcgattt tttaattcct tgtgaaactt ttgtaatatg 12420 aagttgaaat tttgttattg gtaaactata aatgtgtgaa gttggagtat acctttacct 12480 tcttatttgg ctttgtgata gtttaattta tatgtatttt gagttctgac ttgtatttct 12540 ttgaattgat tctagtttaa gtaatcatgg cctcctccga gaacgtcatc accgagttca 12600 tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg 12660 agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg acgaagggcg 12720 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt 12780 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca 12840 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggc gaccgtgacc caggactcct 12900 ccctgcagga cggctgcttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg 12960 acggccccgt gatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc 13020 cccgcgacgg cgtgctgaag ggcgagaccc acaaggccct gaagctgaag gacggcggcc 13080 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct 13140 actactacgt ggacgccaag ctggacatca cctcccacaa cgaggactac accatcgtgg 13200 agcagtacga gcgcaccgag ggccgccacc acctgttcct gtagttttgt gatctgatga 13260 taagtggttg gttcgtgtct catgcacttg ggaggtgatc tatttcacct ggtgtagttt 13320 gtgtttccgt cagttggaaa aacttatccc tatcgatttc gttttcattt tctgcttttc 13380 ttttatgtac cttcgtttgg gcttgtaacg ggcctttgta tttcaactct caataataat 13440 ccaagtgcat gttaaacaat ttgtcatctg tttcggcttt gatatactac tggtgaagat 13500 gggccgtact actgcatcac aacgaaaaat aataataaga tgaaaaactt gaagtggaaa 13560 aaaaaaaaaa cttgaatgtt cactactact cattgaccat aatgtttaac atacatagct 13620 caatagtatt tttgtgaata tggcaacaca aacagtccaa aacaattgtc tcttactata 13680 ccaaaccaag ggcgccgctt gtttgccact ctttgtgtgc aatagtgtga ttaccacatc 13740 tccacattca atatattccc tgaattatct gacgattttg atggctcact gttttcccaa 13800 gtcttgaatt gtcttctgtg cgccagtcaa atgcatatgt gttgagttta tcttttaaat 13860 atcaagcttt tgtttttaac ttttgtttgt aaccaaaaac tcacagtagg agtttgatca 13920 cataatttta tgtttgcctt tgcaatttct agtgagtctt tgattaaaag cttgaaaaga 13980 aaatgcagcc aagcttacca agtaagttat gtgtattaac cagaggaaga gagaatcttg 14040 caaaatttca acaaacacaa aaagaagtat tactacgatt ggtggagaaa gaaaacgatt 14100 ccaaatcttg aactgttgtt gtaaaagcat agcagaaagt gggagacaac cgaaatagaa 14160 atgactataa cttaatttaa tgttatcatt ataatttctt ctagcaaata tttagaaagt 14220 aaatatcaca tcaaccttta atgtaattaa gctttctctt tttgattcat gtgagatgaa 14280 aagaaaaaaa agaagagaaa agtgtagaaa acacatcatt tctaagctga agctgaggta 14340 caaattaatt aataagtgac tagggtcacg tgaccctagt cacttatgat tccgatgact 14400 tcgtaggttc ctagctcaag ccgctcgtgt ccaagcgtca cttacgatta gctaatgatt 14460 acggcatcta ggaccgacta gttcttatgt gcttctagtc tccaaatgtg gttgatagtt 14520 attttgctct aagatcaaca gtaatgaagt ataaatcatc gttgtggtgt gctactcggt 14580 taattgagca ttaacacaca caaacatgac gaggatggta taatctccaa aaatgtgtac 14640 tttgttaggt gggaccctat agccttgatt aatgtgctat gttaggcatg cctggaaacg 14700 tgtgacgcat atgttttgtg aacctgttga tattatatgt gcttttatat taccatattt 14760 tattaaaata ctaatattta ttactagtaa gatataacat tctatctagc ttaaaaacta 14820 accataaata ttccataata actagattta ccaaactaat atactaaata tacataataa 14880 atacaaaatt aacaagacaa taatcaatat ttatgagctt aatatattta gacattatgg 14940 ttggtcgacg ataatcatgc taacttttcg taattgcttg attgaaatat gcttagaata 15000 atgcctcttt gttctacatg gcaaataggg accattatgg tgtaacaccc tgggaaccac 15060 aaacaccccg aaatgctact aaactacaca actaaccttc atatataaaa tttcgacagc 15120 atctcctttg aaaatttgca tagacgtgga agcaacagag tataaacaga tctagctagc 15180 taagtttaaa cggccggcca aacgtgcaag cgctactaga caattcagta cattaaaaac 15240 gtccgcaatg tgttattaag ttgtctaagc gtcaatttat ttacaattga atatatcctg 15300 cca 15303 <210> 165 <211> 15478 <212> DNA <213> Artificial Sequence <220> <223> RV026533 T-DNA <400> 165 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gagtttcaac aacaacaaga tgaagagatg ataaaccatg ttgggaaaat gagcaacaac 1860 acaagtaaca ataataataa taataataac tatttatgta gacaaagtag cacaagatgg 1920 actccaacaa cagaacaaat aaagctttta aaagatcttt attatttaca aggaattaga 1980 tctcctactg ctgatcaaat tcaaagcatt tgtgctaggt tacgtcgtta tggaaaaatt 2040 gaaggcaaaa atgtttttta ttggtttcaa aatcataagg ctagggaaag acaaaaaaag 2100 aagcttactc ctacttctcc taataataat aataataata ataataataa tttcataaga 2160 aataataata ataataataa taatggaaat attaacactt ttcatgctaa atatccttgt 2220 tccttcaata ataatcccgg ggtttcttgt tcaagttcac cagcaggtgt gatttctgga 2280 ggtgggcaat tgatggggag ttttgggtat ggagctgtga ctatggagac taaatttagg 2340 gtaagaggag gtattcctat tccaaatgga gcaaatcaat ggagtaacta tttcaaccca 2400 aacaatccaa catcttactc aaatttcttt attgacaagg tatcatcaaa tgttgatcat 2460 cattatccat acattaacca tcatgatcat gatcatgatc atgatcatca tgatcatgat 2520 cattatggcc cacaatcacc tcatgagatt cagactcttc ctctctttcc catgcacaat 2580 gatcatcatg atctcgatct cgatctcgat ctcgatcacg atcaagggtt ctacacctat 2640 aagcctcaat cggacggctg taattatacc ggaggatatc gatggtcatc taatgttaat 2700 ggtggacata cttctcttga gcttagtctt aactcttatg gatttggatg ctattcacct 2760 aattaaggta cctgacatct tatagtctgc aacctctcgt gtctgaattc ctatctttat 2820 caagtgttat tgcttccacg actataggac agctttcgtc gaaaggtttt gctcatgtga 2880 tctcgaagga ttcatctagt ctgatttttc gtgacttgta tcggttttat tggattcatc 2940 caacatatat caataaaaaa tgagttgtgt ttcctttctt cctagttcag ttaaaattat 3000 ttccctcctg cgcttgtgct gtaattgtct gtgtacctgt tgtttgtgac tgtgttagtt 3060 cccttggata tgatttcgta tttgatatgt acatggagat agcttagctt cattattgga 3120 gtatgaagtt agtatgacat agtcactctc ctggaaaatt gacactgcaa accatatttt 3180 tattctgaac cacaaatcct agtcagtccg ctggcatatg ccgtccgttt gctgaatcca 3240 gaacgtgggt ttggagatgt acggctgaga tgcctctatg cgaaggggat ttcgtggtga 3300 aacgagatgg gagtagagca acgcccgtgg aagatgcttc aaacttccac acttttgagc 3360 aacgatcggc agtagtaagg tagacgattt caagatcaaa gcatatgaag ataaacaaca 3420 tcaacaacaa aatttgttgg ggttctatag agagaaacag agctacatac atacactgtt 3480 ttgtatctac catctgagat gatgaaaaga tgaaaaacta aagaatgccc cggcgccaac 3540 gccaggacac gccgcgcgcg cgtcacccga gccatctctt gacccagccg gcgctgtata 3600 tttacacacg ttgcagcatc gatcaccacc tgttcgatcg cgtcgccgtc accaagcttg 3660 gcgcgccggt cacccggtcc gggcctagaa ggccacccgg ggctcggcgt taggctttta 3720 gtccgctagg tggctagacc gaggttagga cgctagttca ctaggggcct agtggtgttt 3780 taaggatggg caagtttgta caaaaaagca ggctggcgcc ggaaccaatt cagtcgagtg 3840 cggccgccag tgtgatggat atctcgaccc gggtgattgc ggttacatca tgtacggaaa 3900 aataattcta atccttgatt taaatttgaa cttgactatt tatttattct ttatttcatt 3960 ttgtaaatca ttttatgtat ctcctggcaa gcaattttat ccaccttgca ccaacacctt 4020 cgggttccat aatcaaacca ccttaacttc acaccatgct gtaactcaca ccgcccagca 4080 tctccaatgt gaaagaagct aaaatttaat aaacaatcat acgaagcagt gacaaaatac 4140 cagatggtat taatgcttcg ataaaattaa ttggaaagta taaaatggta gaaaataata 4200 aattataatt aatttaagta agataaaaaa taattaaaaa ctaaaatgtt aaaattttaa 4260 aaaaattatt ttaaataata tttaaaaaca ttaaaaatca ttttaaaaaa tttatttata 4320 gaacaattaa ataaatattt cagctaataa aaaacaaaag cttacctagc cttagaagac 4380 aacttgtcca acaattagat gatacccatt gcccttacgt tttctttaac atcaattatt 4440 gtttttgtca acaagctatc ttttagtttt attttattgg taaaaaatat gtcgccttca 4500 agttgcatca tttaacacat ctcgtcatta gaaaaataaa actcttccct aaacgattag 4560 tagaaaaaat cattcgataa taaataagaa agaaaaatta gaaaaaaata acttcatttt 4620 aaaaaaatca ttaaggctat attttttaaa tgactaattt tatatagact gtaactaaaa 4680 gtatacaatt tattatgcta tgtatcttaa agaattactt ataaaaatct acggaagaat 4740 atcttacaaa gtgaaaaaca aatgagaaag aatttagtgg gatgattatg attttatttg 4800 aaaattgaaa aaataattat taaagacttt agtggagtaa gaaagctttc ctattagtct 4860 tttcttatcc ataaaaaaaa aaaaaaaaat ctagcgtgac agcttttcca tagattttaa 4920 taatgtaaaa tactggtagc agccgaccgt tcaggtaatg gacactgtgg tcctaacttg 4980 caacgggtgc gggcccaatt taataacgcc gtggtaacgg ataaagccaa gcgtgaagcg 5040 gtgaaggtac atctctgact ccgtcaagat tacgaaaccg tcaactacga aggactcccc 5100 gaaatatcat ctgtgtcata aacaccaagt cacaccatac atgggcacgc gtcacaatat 5160 gattggagaa cggttccacc gcatatgcta taaaatgccc ccacacccct cgaccctaat 5220 cgcacttcaa ttgcaatcaa attagttcat tctctttgcg cagttcccta cctctccttt 5280 caaggttcgt agatttcttc cgtttttttt tcttcttctt tattgtttgt tctacatcag 5340 catgatgttg atttgattgt gttttctatc gtttcatcga ttataaattt tcataatcag 5400 aagattcagc ttttattaat gcaagaacgt ccttaattga tgattttata accgtaaatt 5460 aggtctaatt agagtttttt tcataaagat tttcagatcc gtttacaaca agccttaatt 5520 gttgattctg tagtcgtaga ttaaggtttt tttcatgaac tacttcagat ccgttaaaca 5580 acagccttat ttgttgatac ttcagtcgtt tttcaagaaa ttgttcagat ccgttgataa 5640 aagccttatt cgttgattct gtatggtatt tcaagagata ttgctcaggt cctttagcaa 5700 ctaccttatt tgttgattct gtggccatag attaggattt tttttcacga aattgcttct 5760 tgaaattacg tgatggattt tgattctgat ttatcttgtg attgttgact ctacagccat 5820 ggcccacagc aagcacggcc tgaaggagga gatgaccatg aagtaccaca tggagggctg 5880 cgtgaacggc cacaagttcg tgatcaccgg cgagggcatc ggctacccct tcaagggcaa 5940 gcagaccatc aacctgtgcg tgatcgaggg cggccccctg cccttcagcg aggacatcct 6000 gagcgccggc ttcaagtacg gcgaccggat cttcaccgag tacccccagg acatcgtgga 6060 ctacttcaag aacagctgcc ccgccggcta cacctggggc cggagcttcc tgttcgagga 6120 cggcgccgtg tgcatctgta acgtggacat caccgtgagc gtgaaggaga actgcatcta 6180 ccacaagagc atcttcaacg gcgtgaactt ccccgccgac ggccccgtga tgaagaagat 6240 gaccaccaac tgggaggcca gctgcgagaa gatcatgccc gtgcctaagc agggcatcct 6300 gaagggcgac gtgagcatgt acctgctgct gaaggacggc ggccggtacc ggtgccagtt 6360 cgacaccgtg tacaaggcca agagcgtgcc cagcaagatg cccgagtggc acttcatcca 6420 gcacaagctg ctgcgggagg accggagcga cgccaagaac cagaagtggc agctgaccga 6480 gcacgccatc gccttcccca gcgccctggc ctgagagctc gaatttcccc gatcgttcaa 6540 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca 6600 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat 6660 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa 6720 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag 6780 atcgggaatt ctagtggccg gcccagctga tatctagacc cagctttctt gtacaaagtg 6840 gccgttaacg gatcggccag aatggcccgg accgggttac cgaattcgag ctcggtaccc 6900 tgggatccga tatcgatggg ccctggccga agcttggtca cccggtccgg gcctagaagg 6960 ccagcttcgg ccgccccggg caactttatt atacaaagtt gatggtacgg atccgtcgac 7020 ggcgcgccgc ggacctgaat tccagcacac tggcggccgt tagtctaaaa acggccccta 7080 actaacgctt agttgcccgc tagattagtc ccccgttagt gcctagttag acacacgacg 7140 aactaggggc tactagtaac ggccgccagt gtgctcggta cggccagaat ggccatctgg 7200 attcagcagg gggtgattgc ggttacatca tgtacggaaa aataattcta atccttgatt 7260 taaatttgaa cttgactatt tatttattct ttatttcatt ttgtaaatca ttttatgtat 7320 ctcctggcaa gcaattttat ccaccttgca ccaacacctt cgggttccat aatcaaacca 7380 ccttaacttc acaccatgct gtaactcaca ccgcccagca tctccaatgt gaaagaagct 7440 aaaatttaat aaacaatcat acgaagcagt gacaaaatac cagatggtat taatgcttcg 7500 ataaaattaa ttggaaagta taaaatggta gaaaataata aattataatt aatttaagta 7560 agataaaaaa taattaaaaa ctaaaatgtt aaaattttaa aaaaattatt ttaaataata 7620 tttaaaaaca ttaaaaatca ttttaaaaaa tttatttata gaacaattaa ataaatattt 7680 cagctaataa aaaacaaaag cttacctagc cttagaagac aacttgtcca acaattagat 7740 gatacccatt gcccttacgt tttctttaac atcaattatt gtttttgtca acaagctatc 7800 ttttagtttt attttattgg taaaaaatat gtcgccttca agttgcatca tttaacacat 7860 ctcgtcatta gaaaaataaa actcttccct aaacgattag tagaaaaaat cattcgataa 7920 taaataagaa agaaaaatta gaaaaaaata acttcatttt aaaaaaatca ttaaggctat 7980 attttttaaa tgactaattt tatatagact gtaactaaaa gtatacaatt tattatgcta 8040 tgtatcttaa agaattactt ataaaaatct acggaagaat atcttacaaa gtgaaaaaca 8100 aatgagaaag aatttagtgg gatgattatg attttatttg aaaattgaaa aaataattat 8160 taaagacttt agtggagtaa gaaagctttc ctattagtct tttcttatcc ataaaaaaaa 8220 aaaaaaaaat ctagcgtgac agcttttcca tagattttaa taatgtaaaa tactggtagc 8280 agccgaccgt tcaggtaatg gacactgtgg tcctaacttg caacgggtgc gggcccaatt 8340 taataacgcc gtggtaacgg ataaagccaa gcgtgaagcg gtgaaggtac atctctgact 8400 ccgtcaagat tacgaaaccg tcaactacga aggactcccc gaaatatcat ctgtgtcata 8460 aacaccaagt cacaccatac atgggcacgc gtcacaatat gattggagaa cggttccacc 8520 gcatatgcta taaaatgccc ccacacccct cgaccctaat cgcacttcaa ttgcaatcaa 8580 attagttcat tctctttgcg cagttcccta cctctccttt caaggttcgt agatttcttc 8640 cgtttttttt tcttcttctt tattgtttgt tctacatcag catgatgttg atttgattgt 8700 gttttctatc gtttcatcga ttataaattt tcataatcag aagattcagc ttttattaat 8760 gcaagaacgt ccttaattga tgattttata accgtaaatt aggtctaatt agagtttttt 8820 tcataaagat tttcagatcc gtttacaaca agccttaatt gttgattctg tagtcgtaga 8880 ttaaggtttt tttcatgaac tacttcagat ccgttaaaca acagccttat ttgttgatac 8940 ttcagtcgtt tttcaagaaa ttgttcagat ccgttgataa aagccttatt cgttgattct 9000 gtatggtatt tcaagagata ttgctcaggt cctttagcaa ctaccttatt tgttgattct 9060 gtggccatag attaggattt tttttcacga aattgcttct tgaaattacg tgatggattt 9120 tgattctgat ttatcttgtg attgttgact ctacagccat ggctgcaact actcttacat 9180 ctgctttacc tggtgctttc agttcatccc agagaccaag tgctcctttc aacttacaaa 9240 gatcacctag agtgcttaga aggttcaaca gaaagacagg acgtcaacct cgtggacttg 9300 ttcgtgctgc aaaggctcaa gcggcgcaaa acctgccaga gaaattcgat gaaacaagac 9360 tctgggaggc cctcagggga tttggggtta gtccaagtag ggttacttac gctcccgtcg 9420 gattcggaga ttaccactgg accgtcacag atgaagacgg gagaccttgg ttcgcaactg 9480 tcagtgacct cgaacacaaa gagcattgcg ggcagggagc acaagcagcc cttaaaggac 9540 tcagacaagc tatggacact gccctcgcac ttagagatag ggacggactc agattcgtgg 9600 ttgctccagt ggcagcaaca gatgggggtg gccctgtcct tccccttgac gctaggtatg 9660 cactcacagt cttccctcac gtcccaggaa gaacaggaga gttcggacaa agactcacag 9720 aggcagagag ggacagactg cttgctcttc tggcagaact ccacggaaga acacctcctg 9780 aaacaacccc accagccgat atggaaccac caggacttcc tggtgttaga gcagcactcg 9840 ccgaaagtga aggaccttgg agtggaggac catttgcaga acccgctaga cttcttctca 9900 gagaacacga ggccacactg cacgcaagac tcgctgaatt cgaagccctc gtcgcaagag 9960 ttaagggtcg tggagcacct cttgtcgtta cccacggaga accacaccca ggcaacttga 10020 tcctcggaga ggaaggctat ctcctcgtgg actgggatac agttggactg gctccagctg 10080 aaagggattt gagtctcatc tcagacgacc cagctgacct cgcaagatac gcagagctta 10140 caggaagaac tccagaccca gacgcactcg cactctatag actgcgttgg agtctccttg 10200 atgtggctga gttcgtcgag tggttcaggg ctgaacacca aagaacccaa gacaccgaga 10260 gtgcatggaa ggggttcact gacacacttg ggcaattggc tgctggtgga tcagctgcat 10320 agcaaactta tctctgttat gaatcagaag aagttcatgt ctcgtttcat ttaaaacttt 10380 ggtggtttgt gttttggggc cttgtaaagc ccctgatgaa taattgttca actatgtttc 10440 cgttcctgtg ttataccttt ctttctaatg agtaatgaca tcaaacttct tctgtattga 10500 aattatgtcc ttgtgagtct ctttatcatc gtttcgtctt tacattatat gtgctacttt 10560 tgtctaatga gcctgaaaag tggctccaat ggtacgcact ggaagatttg ttggcttctg 10620 gtagatatag cgacagtgtt gagcttgtaa tatcatgtct cttattgcta aattagttcc 10680 tttcttaaca gaaaccttca aagtttttgt ttttgttttc atttacctaa tgtacacata 10740 cgctggccat gactaacaac atgtccaggc ttagagcata tttttttcta gcttaaattg 10800 ttaacttgtc attcagtaaa atccgagaat tgtgaagctc taattgaagc taattcgttt 10860 tataaagtca gttaaaaagt atactaaatt atccaacttt tcttcaaaat ctcaaaattc 10920 tatgacaaaa cgatagtctt tgtttatgtc agtaccacaa agaggtggaa aaaaacacca 10980 aaaaaacaat aagcaaacta tacactgaga agaaaaataa aagagagctc aatagatgtt 11040 ttatactaac ggtagattag atcaaagatc caagctttac tctacataga gcagaaccca 11100 gaatcccttc atatctcttt tattctagca ccgataatct actgaaaaga agacacttag 11160 agctctgtct ctttgtcaaa gaagtcccag ccgtcatcca gaagctcctt acgttcatta 11220 acagaggccg caattggatc gggtttactt attttgtggg tatctatact tttattagat 11280 ttttaatcag gctcctgatt tctttttatt tcgattgaat tcctgaactt gtattattca 11340 gtagatcgaa taaattataa aaagataaaa tcataaaata atattttatc ctatcaatca 11400 tattaaagca atgaatatgt aaaattaatc ttatctttat tttaaaaaat catataggtt 11460 tagtattttt ttaaaaataa agataggatt agttttacta ttcactgctt attactttta 11520 aaaaaatcat aaaggtttag tattttttta aaataaatat aggaatagtt ttactattca 11580 ctgctttaat agaaaaatag tttaaaattt aagatagttt taatcccagc atttgccacg 11640 tttgaacgtg agccgaaacg atgtcgttac attatcttaa cctagctgaa acgatgtcgt 11700 cataatatcg ccaaatgcca actggactac gtcgaaccca caaatcccac aaagcgcgtg 11760 aaatcaaatc gctcaaacca caaaaaagaa caacgcgttt gttacacgct caatcccacg 11820 cgagtagagc acagtaacct tcaaataagc gaatggggca taatcagaaa tccgaaataa 11880 acctaggggc attatcggaa atgaaaagta gctcactcaa tataaaaatc taggaaccct 11940 agttttcgtt atcactctgt gctccctcgc tctatttctc agtctctgtg tttgcggctg 12000 aggattccga acgagtgacc ttcttcgttt ctcgcaaagg taacagcctc tgctcttgtc 12060 tcttcgattc gatctatgcc tgtctcttat ttacgatgat gtttcttcgg ttatgttttt 12120 ttatttatgc tttatgctgt tgatgttcgg ttgtttgttt cgctttgttt ttgtggttca 12180 gttttttagg attcttttgg tttttgaatc gattaatcgg aagagatttt cgagttattt 12240 ggtgtgttgg aggtgaatct tttttttgag gtcatagatc tgttgtattt gtgttataaa 12300 catgcgactt tgtatgattt tttacgaggt tatgatgttc tggttgtttt attatgaatc 12360 tgttgagaca gaaccatgat ttttgttgat gttcgtttac actattaaag gtttgtttta 12420 acaggattaa aagtttttta agcatgttga aggagtcttg tagatatgta accgtcgata 12480 gtttttttgt gggtttgttc acatgttatc aagcttaatc ttttactatg tatgcgacca 12540 tatctggatc cagcaaaggc gattttttaa ttccttgtga aacttttgta atatgaagtt 12600 gaaattttgt tattggtaaa ctataaatgt gtgaagttgg agtatacctt taccttctta 12660 tttggctttg tgatagttta atttatatgt attttgagtt ctgacttgta tttctttgaa 12720 ttgattctag tttaagtaat catggcctcc tccgagaacg tcatcaccga gttcatgcgc 12780 ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt tcgagatcga gggcgagggc 12840 gagggccgcc cctacgaggg ccacaacacc gtgaagctga aggtgacgaa gggcggcccc 12900 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg 12960 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg 13020 gagcgcgtga tgaacttcga ggacggcggc gtggcgaccg tgacccagga ctcctccctg 13080 caggacggct gcttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc 13140 cccgtgatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc 13200 gacggcgtgc tgaagggcga gacccacaag gccctgaagc tgaaggacgg cggccactac 13260 ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac 13320 tacgtggacg ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag 13380 tacgagcgca ccgagggccg ccaccacctg ttcctgtagt tttgtgatct gatgataagt 13440 ggttggttcg tgtctcatgc acttgggagg tgatctattt cacctggtgt agtttgtgtt 13500 tccgtcagtt ggaaaaactt atccctatcg atttcgtttt cattttctgc ttttctttta 13560 tgtaccttcg tttgggcttg taacgggcct ttgtatttca actctcaata ataatccaag 13620 tgcatgttaa acaatttgtc atctgtttcg gctttgatat actactggtg aagatgggcc 13680 gtactactgc atcacaacga aaaataataa taagatgaaa aacttgaagt ggaaaaaaaa 13740 aaaaacttga atgttcacta ctactcattg accataatgt ttaacataca tagctcaata 13800 gtatttttgt gaatatggca acacaaacag tccaaaacaa ttgtctctta ctataccaaa 13860 ccaagggcgc cgcttgtttg ccactctttg tgtgcaatag tgtgattacc acatctccac 13920 attcaatata ttccctgaat tatctgacga ttttgatggc tcactgtttt cccaagtctt 13980 gaattgtctt ctgtgcgcca gtcaaatgca tatgtgttga gtttatcttt taaatatcaa 14040 gcttttgttt ttaacttttg tttgtaacca aaaactcaca gtaggagttt gatcacataa 14100 ttttatgttt gcctttgcaa tttctagtga gtctttgatt aaaagcttga aaagaaaatg 14160 cagccaagct taccaagtaa gttatgtgta ttaaccagag gaagagagaa tcttgcaaaa 14220 tttcaacaaa cacaaaaaga agtattacta cgattggtgg agaaagaaaa cgattccaaa 14280 tcttgaactg ttgttgtaaa agcatagcag aaagtgggag acaaccgaaa tagaaatgac 14340 tataacttaa tttaatgtta tcattataat ttcttctagc aaatatttag aaagtaaata 14400 tcacatcaac ctttaatgta attaagcttt ctctttttga ttcatgtgag atgaaaagaa 14460 aaaaaagaag agaaaagtgt agaaaacaca tcatttctaa gctgaagctg aggtacaaat 14520 taattaataa gtgactaggg tcacgtgacc ctagtcactt atgattccga tgacttcgta 14580 ggttcctagc tcaagccgct cgtgtccaag cgtcacttac gattagctaa tgattacggc 14640 atctaggacc gactagttct tatgtgcttc tagtctccaa atgtggttga tagttatttt 14700 gctctaagat caacagtaat gaagtataaa tcatcgttgt ggtgtgctac tcggttaatt 14760 gagcattaac acacacaaac atgacgagga tggtataatc tccaaaaatg tgtactttgt 14820 taggtgggac cctatagcct tgattaatgt gctatgttag gcatgcctgg aaacgtgtga 14880 cgcatatgtt ttgtgaacct gttgatatta tatgtgcttt tatattacca tattttatta 14940 aaatactaat atttattact agtaagatat aacattctat ctagcttaaa aactaaccat 15000 aaatattcca taataactag atttaccaaa ctaatatact aaatatacat aataaataca 15060 aaattaacaa gacaataatc aatatttatg agcttaatat atttagacat tatggttggt 15120 cgacgataat catgctaact tttcgtaatt gcttgattga aatatgctta gaataatgcc 15180 tctttgttct acatggcaaa tagggaccat tatggtgtaa caccctggga accacaaaca 15240 ccccgaaatg ctactaaact acacaactaa ccttcatata taaaatttcg acagcatctc 15300 ctttgaaaat ttgcatagac gtggaagcaa cagagtataa acagatctag ctagctaagt 15360 ttaaacggcc ggccaaacgt gcaagcgcta ctagacaatt cagtacatta aaaacgtccg 15420 caatgtgtta ttaagttgtc taagcgtcaa tttatttaca attgaatata tcctgcca 15478 <210> 166 <211> 15395 <212> DNA <213> Artificial Sequence <220> <223> RV026521 T-DNA <400> 166 aattacaacg gtatatatcc tgtcagccaa caccaaaaca tcaaaaaaat cgggatcagg 60 atcaggatct gcggccgcat cctgcaggtt taaacggccg gccttcacta gctagctgct 120 aagctcaaac gagcaggaag caacgagagg gtggcgcgcg accgacgtgc gtacgtagca 180 tgagcctgag tggagacgtt ggacgtgtat gtatatacct ctctgcgtgt taactatgta 240 cgtaagcggc aggcagtgca ataagtgtgg ctctgtagta tgtacgtgcg ggtacgatgc 300 tgtaagctac tgaggcaagt ccataaataa ataatgacac gtgcgtgttc tataatctct 360 tcgcttcttc atttgtcccc ttgcggagtt tggcatccat tgatgccgtt acgctgagaa 420 cagacacagc agacgaacca aaagtgagtt cttgtatgaa actatgaccc ttcatcgcta 480 ggctcaaaca gcaccccgta cgaacacagc aaattagtca tctaactatt agcccctaca 540 tgtttcagac gatacataaa tatagcccat ccttagcaat tagctattgg ccctgcccat 600 cccaagcaat gatctcgaag tatttttaat atatagtatt tttaatatgt agcttttaaa 660 attagaagat aattttgaga caaaaatctc caagtatttt tttgggtatt ttttactgcc 720 tccgtttttc tttatttctc gtcacctagt ttaattttgt gctaatcggc tataaacgaa 780 acagagagaa aagttactct aaaagcaact ccaacagatt agatataaat cttatatcct 840 gcctagagct gttaaaaaga tagacaactt tagtggatta gtgtatgcaa caaactctcc 900 aaatttaagt atcccaacta cccaacgcat atcgttccct tttcattggc gcacgaactt 960 tcacctgcta tagccgacgt acatgttcgt tttttttggg cggcgcttac tttcttcccc 1020 gttcgttctc agcatcgcaa ctcaatttgt tatggcggag aagcccttgt atcccaggta 1080 gtaatgcaca gatatgcatt attattattc ataaaatcga gctagttacc ctatgaggtg 1140 acatgaagcg ctcacggtta ctatgacggt tagcttcacg actgttggtg gcagtagcgt 1200 acgacttagc tatagttccg gacttaccta actgactaga gtcacactta gctgacccta 1260 gtcacttagg cgcgccaagc ttggatctcc tgcaggatct ggccggccgg atgggcggat 1320 ctcgtacctc aactttgtat agaaaagttg ggccagaatg gcccggaccg ggttaccaag 1380 cggccgcgaa ttccagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcg 1440 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa 1500 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat 1560 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa 1620 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta 1680 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca 1740 tttcatttgg agaggaccca tggaacatcc tgccaaacaa caacaacaag aacaaggaat 1800 taatgatcaa ggtggtgaag gtggtggtgg aagcaatgga aaaggtgggt ttttgtgtag 1860 gcaaagtagc agtcgatgga ctccaacaac tgaccaaata cgaatattga aagagcttta 1920 ttataacaat ggtgttcgct ccccaagcgc tgatcaaatt cagaggatct ctgctaggct 1980 tagacagtac ggtaaaatcg aaggcaaaaa cgttttttat tggtttcaaa accataaggc 2040 tcgtgaaaga cagaagaaaa gattcacttc aaattctact tctcctatta ctacctccaa 2100 tactactaca actactactg attttaaaat ttcttcaact aataataata ttaactggaa 2160 gtctgaagat cattcctcct cttctcataa caaatttcct ccaggaataa caattgcttt 2220 aattgattct tgtttttgca gctccttctt cttcggttat ggtcgccgtt ggccatatgg 2280 gaaattacgg atatgggagt gttcaatatc gtcgggagga aatagcagtg tcgtcggtta 2340 ccggagccac aacatgggat catggatcgg tatcgatccg tattcttctc cggcagctgc 2400 cgcggcgggg ggatcagcca atgtgtttga aaaaacaaaa tatgttgaag aaagcatgga 2460 agatcacgag gagcaagaga tcgaaactct cccccttttc cccattcatg gtgaccggaa 2520 taatctcggt ggtttctgca gcatgaagcc ggaatattca gagagctact acaccaccac 2580 ttggtacggg cggtctgacg ataatggagc cgccggttct cgggcttcgc tggaactcag 2640 cctcaactct tacgccacca tctcacctga tgatggaatg tgaggtacct gacatcttat 2700 agtctgcaac ctctcgtgtc tgaattccta tctttatcaa gtgttattgc ttccacgact 2760 ataggacagc tttcgtcgaa aggttttgct catgtgatct cgaaggattc atctagtctg 2820 atttttcgtg acttgtatcg gttttattgg attcatccaa catatatcaa taaaaaatga 2880 gttgtgtttc ctttcttcct agttcagtta aaattatttc cctcctgcgc ttgtgctgta 2940 attgtctgtg tacctgttgt ttgtgactgt gttagttccc ttggatatga tttcgtattt 3000 gatatgtaca tggagatagc ttagcttcat tattggagta tgaagttagt atgacatagt 3060 cactctcctg gaaaattgac actgcaaacc atatttttat tctgaaccac aaatcctagt 3120 cagtccgctg gcatatgccg tccgtttgct gaatccagaa cgtgggtttg gagatgtacg 3180 gctgagatgc ctctatgcga aggggatttc gtggtgaaac gagatgggag tagagcaacg 3240 cccgtggaag atgcttcaaa cttccacact tttgagcaac gatcggcagt agtaaggtag 3300 acgatttcaa gatcaaagca tatgaagata aacaacatca acaacaaaat ttgttggggt 3360 tctatagaga gaaacagagc tacatacata cactgttttg tatctaccat ctgagatgat 3420 gaaaagatga aaaactaaag aatgccccgg cgccaacgcc aggacacgcc gcgcgcgcgt 3480 cacccgagcc atctcttgac ccagccggcg ctgtatattt acacacgttg cagcatcgat 3540 caccacctgt tcgatcgcgt cgccgtcacc aagcttggcg cgccggtcac ccggtccggg 3600 cctagaaggc cacccggggc tcggcgttag gcttttagtc cgctaggtgg ctagaccgag 3660 gttaggacgc tagttcacta ggggcctagt ggtgttttaa ggatgggcaa gtttgtacaa 3720 aaaagcaggc tggcgccgga accaattcag tcgagtgcgg ccgccagtgt gatggatatc 3780 tcgacccggg tgattgcggt tacatcatgt acggaaaaat aattctaatc cttgatttaa 3840 atttgaactt gactatttat ttattcttta tttcattttg taaatcattt tatgtatctc 3900 ctggcaagca attttatcca ccttgcacca acaccttcgg gttccataat caaaccacct 3960 taacttcaca ccatgctgta actcacaccg cccagcatct ccaatgtgaa agaagctaaa 4020 atttaataaa caatcatacg aagcagtgac aaaataccag atggtattaa tgcttcgata 4080 aaattaattg gaaagtataa aatggtagaa aataataaat tataattaat ttaagtaaga 4140 taaaaaataa ttaaaaacta aaatgttaaa attttaaaaa aattatttta aataatattt 4200 aaaaacatta aaaatcattt taaaaaattt atttatagaa caattaaata aatatttcag 4260 ctaataaaaa acaaaagctt acctagcctt agaagacaac ttgtccaaca attagatgat 4320 acccattgcc cttacgtttt ctttaacatc aattattgtt tttgtcaaca agctatcttt 4380 tagttttatt ttattggtaa aaaatatgtc gccttcaagt tgcatcattt aacacatctc 4440 gtcattagaa aaataaaact cttccctaaa cgattagtag aaaaaatcat tcgataataa 4500 ataagaaaga aaaattagaa aaaaataact tcattttaaa aaaatcatta aggctatatt 4560 ttttaaatga ctaattttat atagactgta actaaaagta tacaatttat tatgctatgt 4620 atcttaaaga attacttata aaaatctacg gaagaatatc ttacaaagtg aaaaacaaat 4680 gagaaagaat ttagtgggat gattatgatt ttatttgaaa attgaaaaaa taattattaa 4740 agactttagt ggagtaagaa agctttccta ttagtctttt cttatccata aaaaaaaaaa 4800 aaaaaatcta gcgtgacagc ttttccatag attttaataa tgtaaaatac tggtagcagc 4860 cgaccgttca ggtaatggac actgtggtcc taacttgcaa cgggtgcggg cccaatttaa 4920 taacgccgtg gtaacggata aagccaagcg tgaagcggtg aaggtacatc tctgactccg 4980 tcaagattac gaaaccgtca actacgaagg actccccgaa atatcatctg tgtcataaac 5040 accaagtcac accatacatg ggcacgcgtc acaatatgat tggagaacgg ttccaccgca 5100 tatgctataa aatgccccca cacccctcga ccctaatcgc acttcaattg caatcaaatt 5160 agttcattct ctttgcgcag ttccctacct ctcctttcaa ggttcgtaga tttcttccgt 5220 ttttttttct tcttctttat tgtttgttct acatcagcat gatgttgatt tgattgtgtt 5280 ttctatcgtt tcatcgatta taaattttca taatcagaag attcagcttt tattaatgca 5340 agaacgtcct taattgatga ttttataacc gtaaattagg tctaattaga gtttttttca 5400 taaagatttt cagatccgtt tacaacaagc cttaattgtt gattctgtag tcgtagatta 5460 aggttttttt catgaactac ttcagatccg ttaaacaaca gccttatttg ttgatacttc 5520 agtcgttttt caagaaattg ttcagatccg ttgataaaag ccttattcgt tgattctgta 5580 tggtatttca agagatattg ctcaggtcct ttagcaacta ccttatttgt tgattctgtg 5640 gccatagatt aggatttttt ttcacgaaat tgcttcttga aattacgtga tggattttga 5700 ttctgattta tcttgtgatt gttgactcta cagccatggc ccacagcaag cacggcctga 5760 aggaggagat gaccatgaag taccacatgg agggctgcgt gaacggccac aagttcgtga 5820 tcaccggcga gggcatcggc taccccttca agggcaagca gaccatcaac ctgtgcgtga 5880 tcgagggcgg ccccctgccc ttcagcgagg acatcctgag cgccggcttc aagtacggcg 5940 accggatctt caccgagtac ccccaggaca tcgtggacta cttcaagaac agctgccccg 6000 ccggctacac ctggggccgg agcttcctgt tcgaggacgg cgccgtgtgc atctgtaacg 6060 tggacatcac cgtgagcgtg aaggagaact gcatctacca caagagcatc ttcaacggcg 6120 tgaacttccc cgccgacggc cccgtgatga agaagatgac caccaactgg gaggccagct 6180 gcgagaagat catgcccgtg cctaagcagg gcatcctgaa gggcgacgtg agcatgtacc 6240 tgctgctgaa ggacggcggc cggtaccggt gccagttcga caccgtgtac aaggccaaga 6300 gcgtgcccag caagatgccc gagtggcact tcatccagca caagctgctg cgggaggacc 6360 ggagcgacgc caagaaccag aagtggcagc tgaccgagca cgccatcgcc ttccccagcg 6420 ccctggcctg agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta 6480 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt 6540 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt 6600 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag 6660 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattcta gtggccggcc 6720 cagctgatat ctagacccag ctttcttgta caaagtggcc gttaacggat cggccagaat 6780 ggcccggacc gggttaccga attcgagctc ggtaccctgg gatccgatat cgatgggccc 6840 tggccgaagc ttggtcaccc ggtccgggcc tagaaggcca gcttcggccg ccccgggcaa 6900 ctttattata caaagttgat ggtacggatc cgtcgacggc gcgccgcgga cctgaattcc 6960 agcacactgg cggccgttag tctaaaaacg gcccctaact aacgcttagt tgcccgctag 7020 attagtcccc cgttagtgcc tagttagaca cacgacgaac taggggctac tagtaacggc 7080 cgccagtgtg ctcggtacgg ccagaatggc catctggatt cagcaggggg tgattgcggt 7140 tacatcatgt acggaaaaat aattctaatc cttgatttaa atttgaactt gactatttat 7200 ttattcttta tttcattttg taaatcattt tatgtatctc ctggcaagca attttatcca 7260 ccttgcacca acaccttcgg gttccataat caaaccacct taacttcaca ccatgctgta 7320 actcacaccg cccagcatct ccaatgtgaa agaagctaaa atttaataaa caatcatacg 7380 aagcagtgac aaaataccag atggtattaa tgcttcgata aaattaattg gaaagtataa 7440 aatggtagaa aataataaat tataattaat ttaagtaaga taaaaaataa ttaaaaacta 7500 aaatgttaaa attttaaaaa aattatttta aataatattt aaaaacatta aaaatcattt 7560 taaaaaattt atttatagaa caattaaata aatatttcag ctaataaaaa acaaaagctt 7620 acctagcctt agaagacaac ttgtccaaca attagatgat acccattgcc cttacgtttt 7680 ctttaacatc aattattgtt tttgtcaaca agctatcttt tagttttatt ttattggtaa 7740 aaaatatgtc gccttcaagt tgcatcattt aacacatctc gtcattagaa aaataaaact 7800 cttccctaaa cgattagtag aaaaaatcat tcgataataa ataagaaaga aaaattagaa 7860 aaaaataact tcattttaaa aaaatcatta aggctatatt ttttaaatga ctaattttat 7920 atagactgta actaaaagta tacaatttat tatgctatgt atcttaaaga attacttata 7980 aaaatctacg gaagaatatc ttacaaagtg aaaaacaaat gagaaagaat ttagtgggat 8040 gattatgatt ttatttgaaa attgaaaaaa taattattaa agactttagt ggagtaagaa 8100 agctttccta ttagtctttt cttatccata aaaaaaaaaa aaaaaatcta gcgtgacagc 8160 ttttccatag attttaataa tgtaaaatac tggtagcagc cgaccgttca ggtaatggac 8220 actgtggtcc taacttgcaa cgggtgcggg cccaatttaa taacgccgtg gtaacggata 8280 aagccaagcg tgaagcggtg aaggtacatc tctgactccg tcaagattac gaaaccgtca 8340 actacgaagg actccccgaa atatcatctg tgtcataaac accaagtcac accatacatg 8400 ggcacgcgtc acaatatgat tggagaacgg ttccaccgca tatgctataa aatgccccca 8460 cacccctcga ccctaatcgc acttcaattg caatcaaatt agttcattct ctttgcgcag 8520 ttccctacct ctcctttcaa ggttcgtaga tttcttccgt ttttttttct tcttctttat 8580 tgtttgttct acatcagcat gatgttgatt tgattgtgtt ttctatcgtt tcatcgatta 8640 taaattttca taatcagaag attcagcttt tattaatgca agaacgtcct taattgatga 8700 ttttataacc gtaaattagg tctaattaga gtttttttca taaagatttt cagatccgtt 8760 tacaacaagc cttaattgtt gattctgtag tcgtagatta aggttttttt catgaactac 8820 ttcagatccg ttaaacaaca gccttatttg ttgatacttc agtcgttttt caagaaattg 8880 ttcagatccg ttgataaaag ccttattcgt tgattctgta tggtatttca agagatattg 8940 ctcaggtcct ttagcaacta ccttatttgt tgattctgtg gccatagatt aggatttttt 9000 ttcacgaaat tgcttcttga aattacgtga tggattttga ttctgattta tcttgtgatt 9060 gttgactcta cagccatggc tgcaactact cttacatctg ctttacctgg tgctttcagt 9120 tcatcccaga gaccaagtgc tcctttcaac ttacaaagat cacctagagt gcttagaagg 9180 ttcaacagaa agacaggacg tcaacctcgt ggacttgttc gtgctgcaaa ggctcaagcg 9240 gcgcaaaacc tgccagagaa attcgatgaa acaagactct gggaggccct caggggattt 9300 ggggttagtc caagtagggt tacttacgct cccgtcggat tcggagatta ccactggacc 9360 gtcacagatg aagacgggag accttggttc gcaactgtca gtgacctcga acacaaagag 9420 cattgcgggc agggagcaca agcagccctt aaaggactca gacaagctat ggacactgcc 9480 ctcgcactta gagataggga cggactcaga ttcgtggttg ctccagtggc agcaacagat 9540 gggggtggcc ctgtccttcc ccttgacgct aggtatgcac tcacagtctt ccctcacgtc 9600 ccaggaagaa caggagagtt cggacaaaga ctcacagagg cagagaggga cagactgctt 9660 gctcttctgg cagaactcca cggaagaaca cctcctgaaa caaccccacc agccgatatg 9720 gaaccaccag gacttcctgg tgttagagca gcactcgccg aaagtgaagg accttggagt 9780 ggaggaccat ttgcagaacc cgctagactt cttctcagag aacacgaggc cacactgcac 9840 gcaagactcg ctgaattcga agccctcgtc gcaagagtta agggtcgtgg agcacctctt 9900 gtcgttaccc acggagaacc acacccaggc aacttgatcc tcggagagga aggctatctc 9960 ctcgtggact gggatacagt tggactggct ccagctgaaa gggatttgag tctcatctca 10020 gacgacccag ctgacctcgc aagatacgca gagcttacag gaagaactcc agacccagac 10080 gcactcgcac tctatagact gcgttggagt ctccttgatg tggctgagtt cgtcgagtgg 10140 ttcagggctg aacaccaaag aacccaagac accgagagtg catggaaggg gttcactgac 10200 acacttgggc aattggctgc tggtggatca gctgcatagc aaacttatct ctgttatgaa 10260 tcagaagaag ttcatgtctc gtttcattta aaactttggt ggtttgtgtt ttggggcctt 10320 gtaaagcccc tgatgaataa ttgttcaact atgtttccgt tcctgtgtta tacctttctt 10380 tctaatgagt aatgacatca aacttcttct gtattgaaat tatgtccttg tgagtctctt 10440 tatcatcgtt tcgtctttac attatatgtg ctacttttgt ctaatgagcc tgaaaagtgg 10500 ctccaatggt acgcactgga agatttgttg gcttctggta gatatagcga cagtgttgag 10560 cttgtaatat catgtctctt attgctaaat tagttccttt cttaacagaa accttcaaag 10620 tttttgtttt tgttttcatt tacctaatgt acacatacgc tggccatgac taacaacatg 10680 tccaggctta gagcatattt ttttctagct taaattgtta acttgtcatt cagtaaaatc 10740 cgagaattgt gaagctctaa ttgaagctaa ttcgttttat aaagtcagtt aaaaagtata 10800 ctaaattatc caacttttct tcaaaatctc aaaattctat gacaaaacga tagtctttgt 10860 ttatgtcagt accacaaaga ggtggaaaaa aacaccaaaa aaacaataag caaactatac 10920 actgagaaga aaaataaaag agagctcaat agatgtttta tactaacggt agattagatc 10980 aaagatccaa gctttactct acatagagca gaacccagaa tcccttcata tctcttttat 11040 tctagcaccg ataatctact gaaaagaaga cacttagagc tctgtctctt tgtcaaagaa 11100 gtcccagccg tcatccagaa gctccttacg ttcattaaca gaggccgcaa ttggatcggg 11160 tttacttatt ttgtgggtat ctatactttt attagatttt taatcaggct cctgatttct 11220 ttttatttcg attgaattcc tgaacttgta ttattcagta gatcgaataa attataaaaa 11280 gataaaatca taaaataata ttttatccta tcaatcatat taaagcaatg aatatgtaaa 11340 attaatctta tctttatttt aaaaaatcat ataggtttag tattttttta aaaataaaga 11400 taggattagt tttactattc actgcttatt acttttaaaa aaatcataaa ggtttagtat 11460 ttttttaaaa taaatatagg aatagtttta ctattcactg ctttaataga aaaatagttt 11520 aaaatttaag atagttttaa tcccagcatt tgccacgttt gaacgtgagc cgaaacgatg 11580 tcgttacatt atcttaacct agctgaaacg atgtcgtcat aatatcgcca aatgccaact 11640 ggactacgtc gaacccacaa atcccacaaa gcgcgtgaaa tcaaatcgct caaaccacaa 11700 aaaagaacaa cgcgtttgtt acacgctcaa tcccacgcga gtagagcaca gtaaccttca 11760 aataagcgaa tggggcataa tcagaaatcc gaaataaacc taggggcatt atcggaaatg 11820 aaaagtagct cactcaatat aaaaatctag gaaccctagt tttcgttatc actctgtgct 11880 ccctcgctct atttctcagt ctctgtgttt gcggctgagg attccgaacg agtgaccttc 11940 ttcgtttctc gcaaaggtaa cagcctctgc tcttgtctct tcgattcgat ctatgcctgt 12000 ctcttattta cgatgatgtt tcttcggtta tgttttttta tttatgcttt atgctgttga 12060 tgttcggttg tttgtttcgc tttgtttttg tggttcagtt ttttaggatt cttttggttt 12120 ttgaatcgat taatcggaag agattttcga gttatttggt gtgttggagg tgaatctttt 12180 ttttgaggtc atagatctgt tgtatttgtg ttataaacat gcgactttgt atgatttttt 12240 acgaggttat gatgttctgg ttgttttatt atgaatctgt tgagacagaa ccatgatttt 12300 tgttgatgtt cgtttacact attaaaggtt tgttttaaca ggattaaaag ttttttaagc 12360 atgttgaagg agtcttgtag atatgtaacc gtcgatagtt tttttgtggg tttgttcaca 12420 tgttatcaag cttaatcttt tactatgtat gcgaccatat ctggatccag caaaggcgat 12480 tttttaattc cttgtgaaac ttttgtaata tgaagttgaa attttgttat tggtaaacta 12540 taaatgtgtg aagttggagt atacctttac cttcttattt ggctttgtga tagtttaatt 12600 tatatgtatt ttgagttctg acttgtattt ctttgaattg attctagttt aagtaatcat 12660 ggcctcctcc gagaacgtca tcaccgagtt catgcgcttc aaggtgcgca tggagggcac 12720 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcca 12780 caacaccgtg aagctgaagg tgacgaaggg cggccccctg cccttcgcct gggacatcct 12840 gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga 12900 ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 12960 cggcggcgtg gcgaccgtga cccaggactc ctccctgcag gacggctgct tcatctacaa 13020 ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtgatgcaga agaagaccat 13080 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagac 13140 ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta 13200 catggccaag aagcccgtgc agctgcccgg ctactactac gtggacgcca agctggacat 13260 cacctcccac aacgaggact acaccatcgt ggagcagtac gagcgcaccg agggccgcca 13320 ccacctgttc ctgtagtttt gtgatctgat gataagtggt tggttcgtgt ctcatgcact 13380 tgggaggtga tctatttcac ctggtgtagt ttgtgtttcc gtcagttgga aaaacttatc 13440 cctatcgatt tcgttttcat tttctgcttt tcttttatgt accttcgttt gggcttgtaa 13500 cgggcctttg tatttcaact ctcaataata atccaagtgc atgttaaaca atttgtcatc 13560 tgtttcggct ttgatatact actggtgaag atgggccgta ctactgcatc acaacgaaaa 13620 ataataataa gatgaaaaac ttgaagtgga aaaaaaaaaa aacttgaatg ttcactacta 13680 ctcattgacc ataatgttta acatacatag ctcaatagta tttttgtgaa tatggcaaca 13740 caaacagtcc aaaacaattg tctcttacta taccaaacca agggcgccgc ttgtttgcca 13800 ctctttgtgt gcaatagtgt gattaccaca tctccacatt caatatattc cctgaattat 13860 ctgacgattt tgatggctca ctgttttccc aagtcttgaa ttgtcttctg tgcgccagtc 13920 aaatgcatat gtgttgagtt tatcttttaa atatcaagct tttgttttta acttttgttt 13980 gtaaccaaaa actcacagta ggagtttgat cacataattt tatgtttgcc tttgcaattt 14040 ctagtgagtc tttgattaaa agcttgaaaa gaaaatgcag ccaagcttac caagtaagtt 14100 atgtgtatta accagaggaa gagagaatct tgcaaaattt caacaaacac aaaaagaagt 14160 attactacga ttggtggaga aagaaaacga ttccaaatct tgaactgttg ttgtaaaagc 14220 atagcagaaa gtgggagaca accgaaatag aaatgactat aacttaattt aatgttatca 14280 ttataatttc ttctagcaaa tatttagaaa gtaaatatca catcaacctt taatgtaatt 14340 aagctttctc tttttgattc atgtgagatg aaaagaaaaa aaagaagaga aaagtgtaga 14400 aaacacatca tttctaagct gaagctgagg tacaaattaa ttaataagtg actagggtca 14460 cgtgacccta gtcacttatg attccgatga cttcgtaggt tcctagctca agccgctcgt 14520 gtccaagcgt cacttacgat tagctaatga ttacggcatc taggaccgac tagttcttat 14580 gtgcttctag tctccaaatg tggttgatag ttattttgct ctaagatcaa cagtaatgaa 14640 gtataaatca tcgttgtggt gtgctactcg gttaattgag cattaacaca cacaaacatg 14700 acgaggatgg tataatctcc aaaaatgtgt actttgttag gtgggaccct atagccttga 14760 ttaatgtgct atgttaggca tgcctggaaa cgtgtgacgc atatgttttg tgaacctgtt 14820 gatattatat gtgcttttat attaccatat tttattaaaa tactaatatt tattactagt 14880 aagatataac attctatcta gcttaaaaac taaccataaa tattccataa taactagatt 14940 taccaaacta atatactaaa tatacataat aaatacaaaa ttaacaagac aataatcaat 15000 atttatgagc ttaatatatt tagacattat ggttggtcga cgataatcat gctaactttt 15060 cgtaattgct tgattgaaat atgcttagaa taatgcctct ttgttctaca tggcaaatag 15120 ggaccattat ggtgtaacac cctgggaacc acaaacaccc cgaaatgcta ctaaactaca 15180 caactaacct tcatatataa aatttcgaca gcatctcctt tgaaaatttg catagacgtg 15240 gaagcaacag agtataaaca gatctagcta gctaagttta aacggccggc caaacgtgca 15300 agcgctacta gacaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa 15360 gcgtcaattt atttacaatt gaatatatcc tgcca 15395 <210> 167 <211> 15349 <212> DNA <213> Artificial Sequence <220> <223> RV026525 T-DNA <400> 167 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcgaatt ccagaagacc 1440 aaagggcaat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt 1500 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat 1560 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca 1620 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 1680 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact 1740 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacccatg 1800 gaatctgctg aaagaatcgg ttcttacgat gtttacagac aaccatcttc taccagatgg 1860 aacccaacct ctgaacaact ttctatcctt agagaacttt actacaccaa cggtatcaga 1920 tctccaaccg ttgatgaaat ccatagaatc tctatgaagc tttctagata cggtaagatc 1980 gaaggtaaga acgtttttta ctggtttcaa aaccataagg ctagacatag acaaaagacc 2040 agactttctg ctatgaacgt tggtaagcca gctgcttctc cagctctttc tcaaaccacc 2100 agaaagcata agcaatgcta catcaccacc aacatcgttc ttacccttaa ccaacattgc 2160 tacaccaaga ccaacatggt tcttcaaatc atctggtctc ttggtcttgg taacaagtct 2220 caaaccacca agatggtttg ggaaatcaag atcaccgctt ttccaaccat ccataccgaa 2280 tctttttacg aaaacagaat ggctggtgaa aactcttctt gcaaggaagc tgcttgctgc 2340 tctcttgaac aaatgtacgc tgttaagcat catgatgtta tgaaggctat ggaagaaaac 2400 caacttcaag aaggtaagat caacagacat gttgaaaccc ttgaactttt tccaacccat 2460 tgcgaagata gatctccaga accatctgaa aagaagtctt gctgctgggg ttcttgcgaa 2520 accaaggaaa accataacaa caacccacca ggtggttaca agggtaagca aagagattct 2580 gttcttgatc tttgcctttc tcttggtaac aagtcttgcg gtcttcatga taactaaggt 2640 acctgacatc ttatagtctg caacctctcg tgtctgaatt cctatcttta tcaagtgtta 2700 ttgcttccac gactatagga cagctttcgt cgaaaggttt tgctcatgtg atctcgaagg 2760 attcatctag tctgattttt cgtgacttgt atcggtttta ttggattcat ccaacatata 2820 tcaataaaaa atgagttgtg tttcctttct tcctagttca gttaaaatta tttccctcct 2880 gcgcttgtgc tgtaattgtc tgtgtacctg ttgtttgtga ctgtgttagt tcccttggat 2940 atgatttcgt atttgatatg tacatggaga tagcttagct tcattattgg agtatgaagt 3000 tagtatgaca tagtcactct cctggaaaat tgacactgca aaccatattt ttattctgaa 3060 ccacaaatcc tagtcagtcc gctggcatat gccgtccgtt tgctgaatcc agaacgtggg 3120 tttggagatg tacggctgag atgcctctat gcgaagggga tttcgtggtg aaacgagatg 3180 ggagtagagc aacgcccgtg gaagatgctt caaacttcca cacttttgag caacgatcgg 3240 cagtagtaag gtagacgatt tcaagatcaa agcatatgaa gataaacaac atcaacaaca 3300 aaatttgttg gggttctata gagagaaaca gagctacata catacactgt tttgtatcta 3360 ccatctgaga tgatgaaaag atgaaaaact aaagaatgcc ccggcgccaa cgccaggaca 3420 cgccgcgcgc gcgtcacccg agccatctct tgacccagcc ggcgctgtat atttacacac 3480 gttgcagcat cgatcaccac ctgttcgatc gcgtcgccgt caccaagctt ggcgcgccgg 3540 tcacccggtc cgggcctaga aggccacccg gggctcggcg ttaggctttt agtccgctag 3600 gtggctagac cgaggttagg acgctagttc actaggggcc tagtggtgtt ttaaggatgg 3660 gcaagtttgt acaaaaaagc aggctggcgc cggaaccaat tcagtcgagt gcggccgcca 3720 gtgtgatgga tatctcgacc cgggtgattg cggttacatc atgtacggaa aaataattct 3780 aatccttgat ttaaatttga acttgactat ttatttattc tttatttcat tttgtaaatc 3840 attttatgta tctcctggca agcaatttta tccaccttgc accaacacct tcgggttcca 3900 taatcaaacc accttaactt cacaccatgc tgtaactcac accgcccagc atctccaatg 3960 tgaaagaagc taaaatttaa taaacaatca tacgaagcag tgacaaaata ccagatggta 4020 ttaatgcttc gataaaatta attggaaagt ataaaatggt agaaaataat aaattataat 4080 taatttaagt aagataaaaa ataattaaaa actaaaatgt taaaatttta aaaaaattat 4140 tttaaataat atttaaaaac attaaaaatc attttaaaaa atttatttat agaacaatta 4200 aataaatatt tcagctaata aaaaacaaaa gcttacctag ccttagaaga caacttgtcc 4260 aacaattaga tgatacccat tgcccttacg ttttctttaa catcaattat tgtttttgtc 4320 aacaagctat cttttagttt tattttattg gtaaaaaata tgtcgccttc aagttgcatc 4380 atttaacaca tctcgtcatt agaaaaataa aactcttccc taaacgatta gtagaaaaaa 4440 tcattcgata ataaataaga aagaaaaatt agaaaaaaat aacttcattt taaaaaaatc 4500 attaaggcta tattttttaa atgactaatt ttatatagac tgtaactaaa agtatacaat 4560 ttattatgct atgtatctta aagaattact tataaaaatc tacggaagaa tatcttacaa 4620 agtgaaaaac aaatgagaaa gaatttagtg ggatgattat gattttattt gaaaattgaa 4680 aaaataatta ttaaagactt tagtggagta agaaagcttt cctattagtc ttttcttatc 4740 cataaaaaaa aaaaaaaaaa tctagcgtga cagcttttcc atagatttta ataatgtaaa 4800 atactggtag cagccgaccg ttcaggtaat ggacactgtg gtcctaactt gcaacgggtg 4860 cgggcccaat ttaataacgc cgtggtaacg gataaagcca agcgtgaagc ggtgaaggta 4920 catctctgac tccgtcaaga ttacgaaacc gtcaactacg aaggactccc cgaaatatca 4980 tctgtgtcat aaacaccaag tcacaccata catgggcacg cgtcacaata tgattggaga 5040 acggttccac cgcatatgct ataaaatgcc cccacacccc tcgaccctaa tcgcacttca 5100 attgcaatca aattagttca ttctctttgc gcagttccct acctctcctt tcaaggttcg 5160 tagatttctt ccgttttttt ttcttcttct ttattgtttg ttctacatca gcatgatgtt 5220 gatttgattg tgttttctat cgtttcatcg attataaatt ttcataatca gaagattcag 5280 cttttattaa tgcaagaacg tccttaattg atgattttat aaccgtaaat taggtctaat 5340 tagagttttt ttcataaaga ttttcagatc cgtttacaac aagccttaat tgttgattct 5400 gtagtcgtag attaaggttt ttttcatgaa ctacttcaga tccgttaaac aacagcctta 5460 tttgttgata cttcagtcgt ttttcaagaa attgttcaga tccgttgata aaagccttat 5520 tcgttgattc tgtatggtat ttcaagagat attgctcagg tcctttagca actaccttat 5580 ttgttgattc tgtggccata gattaggatt ttttttcacg aaattgcttc ttgaaattac 5640 gtgatggatt ttgattctga tttatcttgt gattgttgac tctacagcca tggcccacag 5700 caagcacggc ctgaaggagg agatgaccat gaagtaccac atggagggct gcgtgaacgg 5760 ccacaagttc gtgatcaccg gcgagggcat cggctacccc ttcaagggca agcagaccat 5820 caacctgtgc gtgatcgagg gcggccccct gcccttcagc gaggacatcc tgagcgccgg 5880 cttcaagtac ggcgaccgga tcttcaccga gtacccccag gacatcgtgg actacttcaa 5940 gaacagctgc cccgccggct acacctgggg ccggagcttc ctgttcgagg acggcgccgt 6000 gtgcatctgt aacgtggaca tcaccgtgag cgtgaaggag aactgcatct accacaagag 6060 catcttcaac ggcgtgaact tccccgccga cggccccgtg atgaagaaga tgaccaccaa 6120 ctgggaggcc agctgcgaga agatcatgcc cgtgcctaag cagggcatcc tgaagggcga 6180 cgtgagcatg tacctgctgc tgaaggacgg cggccggtac cggtgccagt tcgacaccgt 6240 gtacaaggcc aagagcgtgc ccagcaagat gcccgagtgg cacttcatcc agcacaagct 6300 gctgcgggag gaccggagcg acgccaagaa ccagaagtgg cagctgaccg agcacgccat 6360 cgccttcccc agcgccctgg cctgagagct cgaatttccc cgatcgttca aacatttggc 6420 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc 6480 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat 6540 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat 6600 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat 6660 tctagtggcc ggcccagctg atatctagac ccagctttct tgtacaaagt ggccgttaac 6720 ggatcggcca gaatggcccg gaccgggtta ccgaattcga gctcggtacc ctgggatccg 6780 atatcgatgg gccctggccg aagcttggtc acccggtccg ggcctagaag gccagcttcg 6840 gccgccccgg gcaactttat tatacaaagt tgatggtacg gatccgtcga cggcgcgccg 6900 cggacctgaa ttccagcaca ctggcggccg ttagtctaaa aacggcccct aactaacgct 6960 tagttgcccg ctagattagt cccccgttag tgcctagtta gacacacgac gaactagggg 7020 ctactagtaa cggccgccag tgtgctcggt acggccagaa tggccatctg gattcagcag 7080 ggggtgattg cggttacatc atgtacggaa aaataattct aatccttgat ttaaatttga 7140 acttgactat ttatttattc tttatttcat tttgtaaatc attttatgta tctcctggca 7200 agcaatttta tccaccttgc accaacacct tcgggttcca taatcaaacc accttaactt 7260 cacaccatgc tgtaactcac accgcccagc atctccaatg tgaaagaagc taaaatttaa 7320 taaacaatca tacgaagcag tgacaaaata ccagatggta ttaatgcttc gataaaatta 7380 attggaaagt ataaaatggt agaaaataat aaattataat taatttaagt aagataaaaa 7440 ataattaaaa actaaaatgt taaaatttta aaaaaattat tttaaataat atttaaaaac 7500 attaaaaatc attttaaaaa atttatttat agaacaatta aataaatatt tcagctaata 7560 aaaaacaaaa gcttacctag ccttagaaga caacttgtcc aacaattaga tgatacccat 7620 tgcccttacg ttttctttaa catcaattat tgtttttgtc aacaagctat cttttagttt 7680 tattttattg gtaaaaaata tgtcgccttc aagttgcatc atttaacaca tctcgtcatt 7740 agaaaaataa aactcttccc taaacgatta gtagaaaaaa tcattcgata ataaataaga 7800 aagaaaaatt agaaaaaaat aacttcattt taaaaaaatc attaaggcta tattttttaa 7860 atgactaatt ttatatagac tgtaactaaa agtatacaat ttattatgct atgtatctta 7920 aagaattact tataaaaatc tacggaagaa tatcttacaa agtgaaaaac aaatgagaaa 7980 gaatttagtg ggatgattat gattttattt gaaaattgaa aaaataatta ttaaagactt 8040 tagtggagta agaaagcttt cctattagtc ttttcttatc cataaaaaaa aaaaaaaaaa 8100 tctagcgtga cagcttttcc atagatttta ataatgtaaa atactggtag cagccgaccg 8160 ttcaggtaat ggacactgtg gtcctaactt gcaacgggtg cgggcccaat ttaataacgc 8220 cgtggtaacg gataaagcca agcgtgaagc ggtgaaggta catctctgac tccgtcaaga 8280 ttacgaaacc gtcaactacg aaggactccc cgaaatatca tctgtgtcat aaacaccaag 8340 tcacaccata catgggcacg cgtcacaata tgattggaga acggttccac cgcatatgct 8400 ataaaatgcc cccacacccc tcgaccctaa tcgcacttca attgcaatca aattagttca 8460 ttctctttgc gcagttccct acctctcctt tcaaggttcg tagatttctt ccgttttttt 8520 ttcttcttct ttattgtttg ttctacatca gcatgatgtt gatttgattg tgttttctat 8580 cgtttcatcg attataaatt ttcataatca gaagattcag cttttattaa tgcaagaacg 8640 tccttaattg atgattttat aaccgtaaat taggtctaat tagagttttt ttcataaaga 8700 ttttcagatc cgtttacaac aagccttaat tgttgattct gtagtcgtag attaaggttt 8760 ttttcatgaa ctacttcaga tccgttaaac aacagcctta tttgttgata cttcagtcgt 8820 ttttcaagaa attgttcaga tccgttgata aaagccttat tcgttgattc tgtatggtat 8880 ttcaagagat attgctcagg tcctttagca actaccttat ttgttgattc tgtggccata 8940 gattaggatt ttttttcacg aaattgcttc ttgaaattac gtgatggatt ttgattctga 9000 tttatcttgt gattgttgac tctacagcca tggctgcaac tactcttaca tctgctttac 9060 ctggtgcttt cagttcatcc cagagaccaa gtgctccttt caacttacaa agatcaccta 9120 gagtgcttag aaggttcaac agaaagacag gacgtcaacc tcgtggactt gttcgtgctg 9180 caaaggctca agcggcgcaa aacctgccag agaaattcga tgaaacaaga ctctgggagg 9240 ccctcagggg atttggggtt agtccaagta gggttactta cgctcccgtc ggattcggag 9300 attaccactg gaccgtcaca gatgaagacg ggagaccttg gttcgcaact gtcagtgacc 9360 tcgaacacaa agagcattgc gggcagggag cacaagcagc ccttaaagga ctcagacaag 9420 ctatggacac tgccctcgca cttagagata gggacggact cagattcgtg gttgctccag 9480 tggcagcaac agatgggggt ggccctgtcc ttccccttga cgctaggtat gcactcacag 9540 tcttccctca cgtcccagga agaacaggag agttcggaca aagactcaca gaggcagaga 9600 gggacagact gcttgctctt ctggcagaac tccacggaag aacacctcct gaaacaaccc 9660 caccagccga tatggaacca ccaggacttc ctggtgttag agcagcactc gccgaaagtg 9720 aaggaccttg gagtggagga ccatttgcag aacccgctag acttcttctc agagaacacg 9780 aggccacact gcacgcaaga ctcgctgaat tcgaagccct cgtcgcaaga gttaagggtc 9840 gtggagcacc tcttgtcgtt acccacggag aaccacaccc aggcaacttg atcctcggag 9900 aggaaggcta tctcctcgtg gactgggata cagttggact ggctccagct gaaagggatt 9960 tgagtctcat ctcagacgac ccagctgacc tcgcaagata cgcagagctt acaggaagaa 10020 ctccagaccc agacgcactc gcactctata gactgcgttg gagtctcctt gatgtggctg 10080 agttcgtcga gtggttcagg gctgaacacc aaagaaccca agacaccgag agtgcatgga 10140 aggggttcac tgacacactt gggcaattgg ctgctggtgg atcagctgca tagcaaactt 10200 atctctgtta tgaatcagaa gaagttcatg tctcgtttca tttaaaactt tggtggtttg 10260 tgttttgggg ccttgtaaag cccctgatga ataattgttc aactatgttt ccgttcctgt 10320 gttatacctt tctttctaat gagtaatgac atcaaacttc ttctgtattg aaattatgtc 10380 cttgtgagtc tctttatcat cgtttcgtct ttacattata tgtgctactt ttgtctaatg 10440 agcctgaaaa gtggctccaa tggtacgcac tggaagattt gttggcttct ggtagatata 10500 gcgacagtgt tgagcttgta atatcatgtc tcttattgct aaattagttc ctttcttaac 10560 agaaaccttc aaagtttttg tttttgtttt catttaccta atgtacacat acgctggcca 10620 tgactaacaa catgtccagg cttagagcat atttttttct agcttaaatt gttaacttgt 10680 cattcagtaa aatccgagaa ttgtgaagct ctaattgaag ctaattcgtt ttataaagtc 10740 agttaaaaag tatactaaat tatccaactt ttcttcaaaa tctcaaaatt ctatgacaaa 10800 acgatagtct ttgtttatgt cagtaccaca aagaggtgga aaaaaacacc aaaaaaacaa 10860 taagcaaact atacactgag aagaaaaata aaagagagct caatagatgt tttatactaa 10920 cggtagatta gatcaaagat ccaagcttta ctctacatag agcagaaccc agaatccctt 10980 catatctctt ttattctagc accgataatc tactgaaaag aagacactta gagctctgtc 11040 tctttgtcaa agaagtccca gccgtcatcc agaagctcct tacgttcatt aacagaggcc 11100 gcaattggat cgggtttact tattttgtgg gtatctatac ttttattaga tttttaatca 11160 ggctcctgat ttctttttat ttcgattgaa ttcctgaact tgtattattc agtagatcga 11220 ataaattata aaaagataaa atcataaaat aatattttat cctatcaatc atattaaagc 11280 aatgaatatg taaaattaat cttatcttta ttttaaaaaa tcatataggt ttagtatttt 11340 tttaaaaata aagataggat tagttttact attcactgct tattactttt aaaaaaatca 11400 taaaggttta gtattttttt aaaataaata taggaatagt tttactattc actgctttaa 11460 tagaaaaata gtttaaaatt taagatagtt ttaatcccag catttgccac gtttgaacgt 11520 gagccgaaac gatgtcgtta cattatctta acctagctga aacgatgtcg tcataatatc 11580 gccaaatgcc aactggacta cgtcgaaccc acaaatccca caaagcgcgt gaaatcaaat 11640 cgctcaaacc acaaaaaaga acaacgcgtt tgttacacgc tcaatcccac gcgagtagag 11700 cacagtaacc ttcaaataag cgaatggggc ataatcagaa atccgaaata aacctagggg 11760 cattatcgga aatgaaaagt agctcactca atataaaaat ctaggaaccc tagttttcgt 11820 tatcactctg tgctccctcg ctctatttct cagtctctgt gtttgcggct gaggattccg 11880 aacgagtgac cttcttcgtt tctcgcaaag gtaacagcct ctgctcttgt ctcttcgatt 11940 cgatctatgc ctgtctctta tttacgatga tgtttcttcg gttatgtttt tttatttatg 12000 ctttatgctg ttgatgttcg gttgtttgtt tcgctttgtt tttgtggttc agttttttag 12060 gattcttttg gtttttgaat cgattaatcg gaagagattt tcgagttatt tggtgtgttg 12120 gaggtgaatc ttttttttga ggtcatagat ctgttgtatt tgtgttataa acatgcgact 12180 ttgtatgatt ttttacgagg ttatgatgtt ctggttgttt tattatgaat ctgttgagac 12240 agaaccatga tttttgttga tgttcgttta cactattaaa ggtttgtttt aacaggatta 12300 aaagtttttt aagcatgttg aaggagtctt gtagatatgt aaccgtcgat agtttttttg 12360 tgggtttgtt cacatgttat caagcttaat cttttactat gtatgcgacc atatctggat 12420 ccagcaaagg cgatttttta attccttgtg aaacttttgt aatatgaagt tgaaattttg 12480 ttattggtaa actataaatg tgtgaagttg gagtatacct ttaccttctt atttggcttt 12540 gtgatagttt aatttatatg tattttgagt tctgacttgt atttctttga attgattcta 12600 gtttaagtaa tcatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg 12660 cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 12720 ccctacgagg gccacaacac cgtgaagctg aaggtgacga agggcggccc cctgcccttc 12780 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc 12840 gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 12900 atgaacttcg aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc 12960 tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg 13020 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg 13080 ctgaagggcg agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag 13140 ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac 13200 gccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc 13260 accgagggcc gccaccacct gttcctgtag ttttgtgatc tgatgataag tggttggttc 13320 gtgtctcatg cacttgggag gtgatctatt tcacctggtg tagtttgtgt ttccgtcagt 13380 tggaaaaact tatccctatc gatttcgttt tcattttctg cttttctttt atgtaccttc 13440 gtttgggctt gtaacgggcc tttgtatttc aactctcaat aataatccaa gtgcatgtta 13500 aacaatttgt catctgtttc ggctttgata tactactggt gaagatgggc cgtactactg 13560 catcacaacg aaaaataata ataagatgaa aaacttgaag tggaaaaaaa aaaaaacttg 13620 aatgttcact actactcatt gaccataatg tttaacatac atagctcaat agtatttttg 13680 tgaatatggc aacacaaaca gtccaaaaca attgtctctt actataccaa accaagggcg 13740 ccgcttgttt gccactcttt gtgtgcaata gtgtgattac cacatctcca cattcaatat 13800 attccctgaa ttatctgacg attttgatgg ctcactgttt tcccaagtct tgaattgtct 13860 tctgtgcgcc agtcaaatgc atatgtgttg agtttatctt ttaaatatca agcttttgtt 13920 tttaactttt gtttgtaacc aaaaactcac agtaggagtt tgatcacata attttatgtt 13980 tgcctttgca atttctagtg agtctttgat taaaagcttg aaaagaaaat gcagccaagc 14040 ttaccaagta agttatgtgt attaaccaga ggaagagaga atcttgcaaa atttcaacaa 14100 acacaaaaag aagtattact acgattggtg gagaaagaaa acgattccaa atcttgaact 14160 gttgttgtaa aagcatagca gaaagtggga gacaaccgaa atagaaatga ctataactta 14220 atttaatgtt atcattataa tttcttctag caaatattta gaaagtaaat atcacatcaa 14280 cctttaatgt aattaagctt tctctttttg attcatgtga gatgaaaaga aaaaaaagaa 14340 gagaaaagtg tagaaaacac atcatttcta agctgaagct gaggtacaaa ttaattaata 14400 agtgactagg gtcacgtgac cctagtcact tatgattccg atgacttcgt aggttcctag 14460 ctcaagccgc tcgtgtccaa gcgtcactta cgattagcta atgattacgg catctaggac 14520 cgactagttc ttatgtgctt ctagtctcca aatgtggttg atagttattt tgctctaaga 14580 tcaacagtaa tgaagtataa atcatcgttg tggtgtgcta ctcggttaat tgagcattaa 14640 cacacacaaa catgacgagg atggtataat ctccaaaaat gtgtactttg ttaggtggga 14700 ccctatagcc ttgattaatg tgctatgtta ggcatgcctg gaaacgtgtg acgcatatgt 14760 tttgtgaacc tgttgatatt atatgtgctt ttatattacc atattttatt aaaatactaa 14820 tatttattac tagtaagata taacattcta tctagcttaa aaactaacca taaatattcc 14880 ataataacta gatttaccaa actaatatac taaatataca taataaatac aaaattaaca 14940 agacaataat caatatttat gagcttaata tatttagaca ttatggttgg tcgacgataa 15000 tcatgctaac ttttcgtaat tgcttgattg aaatatgctt agaataatgc ctctttgttc 15060 tacatggcaa atagggacca ttatggtgta acaccctggg aaccacaaac accccgaaat 15120 gctactaaac tacacaacta accttcatat ataaaatttc gacagcatct cctttgaaaa 15180 tttgcataga cgtggaagca acagagtata aacagatcta gctagctaag tttaaacggc 15240 cggccaaacg tgcaagcgct actagacaat tcagtacatt aaaaacgtcc gcaatgtgtt 15300 attaagttgt ctaagcgtca atttatttac aattgaatat atcctgcca 15349 <210> 168 <211> 11996 <212> DNA <213> Artificial Sequence <220> <223> RV022814 T-DNA <400> 168 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 1440 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggtcgaccga 1500 cagccttcca aatgttcttc tcaaacggaa tcgtcgtatc cagcctactc gctattgtcc 1560 tcaatgccgt attaaatcat aaaaagaaat aagaaaaaga ggtgcgagcc tcttttttgt 1620 gtgacaaaat aaaaacatct acctattcat atacgctagt gtcatagtcc tgaaaatcat 1680 ctgcatcaag aacaatttca caactcttat acttttctct tacaagtcgt tcggcttcat 1740 ctggattttc agcctctata cttactaaac gtgataaagt ttctgtaatt tctactgtat 1800 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg 1860 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata 1920 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata 1980 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg 2040 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga 2100 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt 2160 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg 2220 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct 2280 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca 2340 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca 2400 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg 2460 cttttagtaa gccggatcca cgcggcgttt acgccccccc tgccactcat cgcagtactg 2520 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg 2580 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac 2640 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca 2700 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt 2760 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg 2820 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg 2880 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg 2940 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt 3000 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg 3060 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt 3120 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa 3180 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac 3240 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg 3300 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca 3360 aagtgcgtcg ggtgatgctg ccaacttacg gccgccatag tgactggata tgttgtgttt 3420 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat 3480 cattttacgt ttctcgttca actttattat acaaagttga tggtacggat ccgtcgacgg 3540 cgcgccgcgg acctgaattc cagcacactg gcggccgtta gtctaaaaac ggcccctaac 3600 taacgcttag ttgcccgcta gattagtccc ccgttagtgc ctagttagac acacgacgaa 3660 ctaggggcta ctagtaacgg ccgccagtgt gctcggtacg gccagaatgg ccatctggat 3720 tcagcagggg gtgattgcgg ttacatcatg tacggaaaaa taattctaat ccttgattta 3780 aatttgaact tgactattta tttattcttt atttcatttt gtaaatcatt ttatgtatct 3840 cctggcaagc aattttatcc accttgcacc aacaccttcg ggttccataa tcaaaccacc 3900 ttaacttcac accatgctgt aactcacacc gcccagcatc tccaatgtga aagaagctaa 3960 aatttaataa acaatcatac gaagcagtga caaaatacca gatggtatta atgcttcgat 4020 aaaattaatt ggaaagtata aaatggtaga aaataataaa ttataattaa tttaagtaag 4080 ataaaaaata attaaaaact aaaatgttaa aattttaaaa aaattatttt aaataatatt 4140 taaaaacatt aaaaatcatt ttaaaaaatt tatttataga acaattaaat aaatatttca 4200 gctaataaaa aacaaaagct tacctagcct tagaagacaa cttgtccaac aattagatga 4260 tacccattgc ccttacgttt tctttaacat caattattgt ttttgtcaac aagctatctt 4320 ttagttttat tttattggta aaaaatatgt cgccttcaag ttgcatcatt taacacatct 4380 cgtcattaga aaaataaaac tcttccctaa acgattagta gaaaaaatca ttcgataata 4440 aataagaaag aaaaattaga aaaaaataac ttcattttaa aaaaatcatt aaggctatat 4500 tttttaaatg actaatttta tatagactgt aactaaaagt atacaattta ttatgctatg 4560 tatcttaaag aattacttat aaaaatctac ggaagaatat cttacaaagt gaaaaacaaa 4620 tgagaaagaa tttagtggga tgattatgat tttatttgaa aattgaaaaa ataattatta 4680 aagactttag tggagtaaga aagctttcct attagtcttt tcttatccat aaaaaaaaaa 4740 aaaaaaatct agcgtgacag cttttccata gattttaata atgtaaaata ctggtagcag 4800 ccgaccgttc aggtaatgga cactgtggtc ctaacttgca acgggtgcgg gcccaattta 4860 ataacgccgt ggtaacggat aaagccaagc gtgaagcggt gaaggtacat ctctgactcc 4920 gtcaagatta cgaaaccgtc aactacgaag gactccccga aatatcatct gtgtcataaa 4980 caccaagtca caccatacat gggcacgcgt cacaatatga ttggagaacg gttccaccgc 5040 atatgctata aaatgccccc acacccctcg accctaatcg cacttcaatt gcaatcaaat 5100 tagttcattc tctttgcgca gttccctacc tctcctttca aggttcgtag atttcttccg 5160 tttttttttc ttcttcttta ttgtttgttc tacatcagca tgatgttgat ttgattgtgt 5220 tttctatcgt ttcatcgatt ataaattttc ataatcagaa gattcagctt ttattaatgc 5280 aagaacgtcc ttaattgatg attttataac cgtaaattag gtctaattag agtttttttc 5340 ataaagattt tcagatccgt ttacaacaag ccttaattgt tgattctgta gtcgtagatt 5400 aaggtttttt tcatgaacta cttcagatcc gttaaacaac agccttattt gttgatactt 5460 cagtcgtttt tcaagaaatt gttcagatcc gttgataaaa gccttattcg ttgattctgt 5520 atggtatttc aagagatatt gctcaggtcc tttagcaact accttatttg ttgattctgt 5580 ggccatagat taggattttt tttcacgaaa ttgcttcttg aaattacgtg atggattttg 5640 attctgattt atcttgtgat tgttgactct acagccatgg ctgcaactac tcttacatct 5700 gctttacctg gtgctttcag ttcatcccag agaccaagtg ctcctttcaa cttacaaaga 5760 tcacctagag tgcttagaag gttcaacaga aagacaggac gtcaacctcg tggacttgtt 5820 cgtgctgcaa aggctcaagc ggcgcaaaac ctgccagaga aattcgatga aacaagactc 5880 tgggaggccc tcaggggatt tggggttagt ccaagtaggg ttacttacgc tcccgtcgga 5940 ttcggagatt accactggac cgtcacagat gaagacggga gaccttggtt cgcaactgtc 6000 agtgacctcg aacacaaaga gcattgcggg cagggagcac aagcagccct taaaggactc 6060 agacaagcta tggacactgc cctcgcactt agagataggg acggactcag attcgtggtt 6120 gctccagtgg cagcaacaga tgggggtggc cctgtccttc cccttgacgc taggtatgca 6180 ctcacagtct tccctcacgt cccaggaaga acaggagagt tcggacaaag actcacagag 6240 gcagagaggg acagactgct tgctcttctg gcagaactcc acggaagaac acctcctgaa 6300 acaaccccac cagccgatat ggaaccacca ggacttcctg gtgttagagc agcactcgcc 6360 gaaagtgaag gaccttggag tggaggacca tttgcagaac ccgctagact tcttctcaga 6420 gaacacgagg ccacactgca cgcaagactc gctgaattcg aagccctcgt cgcaagagtt 6480 aagggtcgtg gagcacctct tgtcgttacc cacggagaac cacacccagg caacttgatc 6540 ctcggagagg aaggctatct cctcgtggac tgggatacag ttggactggc tccagctgaa 6600 agggatttga gtctcatctc agacgaccca gctgacctcg caagatacgc agagcttaca 6660 ggaagaactc cagacccaga cgcactcgca ctctatagac tgcgttggag tctccttgat 6720 gtggctgagt tcgtcgagtg gttcagggct gaacaccaaa gaacccaaga caccgagagt 6780 gcatggaagg ggttcactga cacacttggg caattggctg ctggtggatc agctgcatag 6840 caaacttatc tctgttatga atcagaagaa gttcatgtct cgtttcattt aaaactttgg 6900 tggtttgtgt tttggggcct tgtaaagccc ctgatgaata attgttcaac tatgtttccg 6960 ttcctgtgtt atacctttct ttctaatgag taatgacatc aaacttcttc tgtattgaaa 7020 ttatgtcctt gtgagtctct ttatcatcgt ttcgtcttta cattatatgt gctacttttg 7080 tctaatgagc ctgaaaagtg gctccaatgg tacgcactgg aagatttgtt ggcttctggt 7140 agatatagcg acagtgttga gcttgtaata tcatgtctct tattgctaaa ttagttcctt 7200 tcttaacaga aaccttcaaa gtttttgttt ttgttttcat ttacctaatg tacacatacg 7260 ctggccatga ctaacaacat gtccaggctt agagcatatt tttttctagc ttaaattgtt 7320 aacttgtcat tcagtaaaat ccgagaattg tgaagctcta attgaagcta attcgtttta 7380 taaagtcagt taaaaagtat actaaattat ccaacttttc ttcaaaatct caaaattcta 7440 tgacaaaacg atagtctttg tttatgtcag taccacaaag aggtggaaaa aaacaccaaa 7500 aaaacaataa gcaaactata cactgagaag aaaaataaaa gagagctcaa tagatgtttt 7560 atactaacgg tagattagat caaagatcca agctttactc tacatagagc agaacccaga 7620 atcccttcat atctctttta ttctagcacc gataatctac tgaaaagaag acacttagag 7680 ctctgtctct ttgtcaaaga agtcccagcc gtcatccaga agctccttac gttcattaac 7740 agaggccgca attggatcgg gtttacttat tttgtgggta tctatacttt tattagattt 7800 ttaatcaggc tcctgatttc tttttatttc gattgaattc ctgaacttgt attattcagt 7860 agatcgaata aattataaaa agataaaatc ataaaataat attttatcct atcaatcata 7920 ttaaagcaat gaatatgtaa aattaatctt atctttattt taaaaaatca tataggttta 7980 gtattttttt aaaaataaag ataggattag ttttactatt cactgcttat tacttttaaa 8040 aaaatcataa aggtttagta tttttttaaa ataaatatag gaatagtttt actattcact 8100 gctttaatag aaaaatagtt taaaatttaa gatagtttta atcccagcat ttgccacgtt 8160 tgaacgtgag ccgaaacgat gtcgttacat tatcttaacc tagctgaaac gatgtcgtca 8220 taatatcgcc aaatgccaac tggactacgt cgaacccaca aatcccacaa agcgcgtgaa 8280 atcaaatcgc tcaaaccaca aaaaagaaca acgcgtttgt tacacgctca atcccacgcg 8340 agtagagcac agtaaccttc aaataagcga atggggcata atcagaaatc cgaaataaac 8400 ctaggggcat tatcggaaat gaaaagtagc tcactcaata taaaaatcta ggaaccctag 8460 ttttcgttat cactctgtgc tccctcgctc tatttctcag tctctgtgtt tgcggctgag 8520 gattccgaac gagtgacctt cttcgtttct cgcaaaggta acagcctctg ctcttgtctc 8580 ttcgattcga tctatgcctg tctcttattt acgatgatgt ttcttcggtt atgttttttt 8640 atttatgctt tatgctgttg atgttcggtt gtttgtttcg ctttgttttt gtggttcagt 8700 tttttaggat tcttttggtt tttgaatcga ttaatcggaa gagattttcg agttatttgg 8760 tgtgttggag gtgaatcttt tttttgaggt catagatctg ttgtatttgt gttataaaca 8820 tgcgactttg tatgattttt tacgaggtta tgatgttctg gttgttttat tatgaatctg 8880 ttgagacaga accatgattt ttgttgatgt tcgtttacac tattaaaggt ttgttttaac 8940 aggattaaaa gttttttaag catgttgaag gagtcttgta gatatgtaac cgtcgatagt 9000 ttttttgtgg gtttgttcac atgttatcaa gcttaatctt ttactatgta tgcgaccata 9060 tctggatcca gcaaaggcga ttttttaatt ccttgtgaaa cttttgtaat atgaagttga 9120 aattttgtta ttggtaaact ataaatgtgt gaagttggag tataccttta ccttcttatt 9180 tggctttgtg atagtttaat ttatatgtat tttgagttct gacttgtatt tctttgaatt 9240 gattctagtt taagtaatca tggcctcctc cgagaacgtc atcaccgagt tcatgcgctt 9300 caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga 9360 gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgacgaagg gcggccccct 9420 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa 9480 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga 9540 gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact cctccctgca 9600 ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc 9660 cgtgatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga 9720 cggcgtgctg aagggcgaga cccacaaggc cctgaagctg aaggacggcg gccactacct 9780 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta 9840 cgtggacgcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta 9900 cgagcgcacc gagggccgcc accacctgtt cctgtagttt tgtgatctga tgataagtgg 9960 ttggttcgtg tctcatgcac ttgggaggtg atctatttca cctggtgtag tttgtgtttc 10020 cgtcagttgg aaaaacttat ccctatcgat ttcgttttca ttttctgctt ttcttttatg 10080 taccttcgtt tgggcttgta acgggccttt gtatttcaac tctcaataat aatccaagtg 10140 catgttaaac aatttgtcat ctgtttcggc tttgatatac tactggtgaa gatgggccgt 10200 actactgcat cacaacgaaa aataataata agatgaaaaa cttgaagtgg aaaaaaaaaa 10260 aaacttgaat gttcactact actcattgac cataatgttt aacatacata gctcaatagt 10320 atttttgtga atatggcaac acaaacagtc caaaacaatt gtctcttact ataccaaacc 10380 aagggcgccg cttgtttgcc actctttgtg tgcaatagtg tgattaccac atctccacat 10440 tcaatatatt ccctgaatta tctgacgatt ttgatggctc actgttttcc caagtcttga 10500 attgtcttct gtgcgccagt caaatgcata tgtgttgagt ttatctttta aatatcaagc 10560 ttttgttttt aacttttgtt tgtaaccaaa aactcacagt aggagtttga tcacataatt 10620 ttatgtttgc ctttgcaatt tctagtgagt ctttgattaa aagcttgaaa agaaaatgca 10680 gccaagctta ccaagtaagt tatgtgtatt aaccagagga agagagaatc ttgcaaaatt 10740 tcaacaaaca caaaaagaag tattactacg attggtggag aaagaaaacg attccaaatc 10800 ttgaactgtt gttgtaaaag catagcagaa agtgggagac aaccgaaata gaaatgacta 10860 taacttaatt taatgttatc attataattt cttctagcaa atatttagaa agtaaatatc 10920 acatcaacct ttaatgtaat taagctttct ctttttgatt catgtgagat gaaaagaaaa 10980 aaaagaagag aaaagtgtag aaaacacatc atttctaagc tgaagctgag gtacaaatta 11040 attaataagt gactagggtc acgtgaccct agtcacttat gattccgatg acttcgtagg 11100 ttcctagctc aagccgctcg tgtccaagcg tcacttacga ttagctaatg attacggcat 11160 ctaggaccga ctagttctta tgtgcttcta gtctccaaat gtggttgata gttattttgc 11220 tctaagatca acagtaatga agtataaatc atcgttgtgg tgtgctactc ggttaattga 11280 gcattaacac acacaaacat gacgaggatg gtataatctc caaaaatgtg tactttgtta 11340 ggtgggaccc tatagccttg attaatgtgc tatgttaggc atgcctggaa acgtgtgacg 11400 catatgtttt gtgaacctgt tgatattata tgtgctttta tattaccata ttttattaaa 11460 atactaatat ttattactag taagatataa cattctatct agcttaaaaa ctaaccataa 11520 atattccata ataactagat ttaccaaact aatatactaa atatacataa taaatacaaa 11580 attaacaaga caataatcaa tatttatgag cttaatatat ttagacatta tggttggtcg 11640 acgataatca tgctaacttt tcgtaattgc ttgattgaaa tatgcttaga ataatgcctc 11700 tttgttctac atggcaaata gggaccatta tggtgtaaca ccctgggaac cacaaacacc 11760 ccgaaatgct actaaactac acaactaacc ttcatatata aaatttcgac agcatctcct 11820 ttgaaaattt gcatagacgt ggaagcaaca gagtataaac agatctagct agctaagttt 11880 aaacggccgg ccaaacgtgc aagcgctact agacaattca gtacattaaa aacgtccgca 11940 atgtgttatt aagttgtcta agcgtcaatt tatttacaat tgaatatatc ctgcca 11996 <210> 169 <211> 16534 <212> DNA <213> Artificial Sequence <220> <223> RV027344 T-DNA <400> 169 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgctatta aactaattaa 1440 acttagcctt tttcattagt tttgttactg gaaacggttc gtctttagtg cacaaagacc 1500 aaggtcaatt taattcttta ataaattcaa ttgagagatc agaagcttgt gaatctggtt 1560 ctctctctct cttctcttac tagcttttga gtcgacaaaa gagagagaaa agatcattaa 1620 actcgaatcc aaaacttcaa ccaacaaaag ggaaaaaaag aaaaaataac tacaaagaga 1680 tctatcttta gggtttcaaa actttcttga gtttcagatc gaattgagta agtatttata 1740 ttatttaagt gttcttctta ctgtatcatt tctgaccata caccaaacta gggtttctta 1800 aagatcataa ctctctctct ctcgcttctt cctacactag atctgtccga tagatctgat 1860 gctttgagaa gtcttgtgac tttcaagatt gatttttctt tttgtttgtt tgtttatatg 1920 tacacatact tggagataga tatactctca tatatgttac tgtgcatatg gatggatcca 1980 tcttaagtgt gaataaaaac tacccaaatc tcatttttgt ttgtttctta tctcatttgt 2040 tgtttttgta aggccgtcat tataaatcta aaacaattta tccgttttcg taaagaaaat 2100 cgctttccaa aaaaaaaaac tttgaagtaa aaatatcatt ttgggtctat attcaaatct 2160 ctcctcatat ttccatctca ttatttgtaa aattaatata ttaggtcatt atcatattga 2220 ttatagagtt tatatgttat gttataaagt aaaagttata tatgtaaata ttacgtttgt 2280 aatatatata tttatacata tgtacgtata cataatttgt atgtatatgt gcctcatttt 2340 taacaaatat tatcgttaat ctttagtttc attataattt tttagggatg tatacattat 2400 ataaatattt tatgttattt atatctgaaa tttctatact ataacatctt agatagtttt 2460 catacgtaat taagagtttt aattaatagt tacataatta tgattagatg aatttatcta 2520 taaattgtat gcaaaaatat atacatatcc aaccgaaaaa atggagagta aacaaaaacc 2580 taacaattaa tggtttagta cttgtgaaaa tgataaaatt attaatttaa tatatagttt 2640 cataacagct agaaataact ttcccttaat ataattattt tcttccattt ccataatgat 2700 tttcttcttc ttcattctgt atacatatat gagcgtttca tatataatct tttactaatt 2760 cgattgtgat tttgattttg ggcagtggaa gatatacttg gagaagaaaa tcaaatcacg 2820 aaaatatatt gatcagtgcc ttgaaggaaa ccaaagttga caactccaaa aatccatttc 2880 gttattaata tctaactgag tgatcatagt caatcatcca taacaccatg gaaactcaac 2940 aacaacaagc agatattcaa gattttggga acaaaaacag taacacctat gcatgcaggc 3000 aaagcagtac taggtggaca cccacaagtg atcagatcag aatactgaaa gagctctact 3060 acaacaatgg aatcagatca ccaactgctg atcagattca gagaatcgct gctcaactca 3120 gacagtacgg caagattgaa ggcaagaacg tgttttattg gtttcagaac cataaagctc 3180 gtgaacgcca gaagaaacgg ttcactccta ctgctcctcc accaccgtct cacttctccg 3240 atcatattaa ccaccatctt cccaacgctg cccccatgca gattccgtct caccaccacc 3300 actaccacca tcaagaacca ccacatgtct atgctcaccc tcacaaactc tacaccactc 3360 atcacattgg ggttggttct tcttcacaag gagtgatggg tgtagggtgt ggctatggat 3420 ctgttgctat ggagaagagt ttcaggaagt gttcaatatc accaccggga gagagtaagg 3480 ccacaggagg aattggccgg aatatcggtt caagatcacg gataagtgtc gattcgtgtt 3540 ccttcttcga cacaattaag ccaaagacat atgagatgtt tgagaatcat gatcaagatg 3600 aagaacaagg agagccttca actgagatcg aaacacttcc attattcccg attcacgacg 3660 gcaaccatca tgatttcttc ggcatgagga cggcaaatct gcaattggag caaggcactg 3720 gaggttacta cactggagga agctggtatc ggtctgatga gagggcgtct cttgagctca 3780 gtctcaactc atatggttat tataactaat tcgaactggc caggtacctg acatcttata 3840 gtctgcaacc tctcgtgtct gaattcctat ctttatcaag tgttattgct tccacgacta 3900 taggacagct ttcgtcgaaa ggttttgctc atgtgatctc gaaggattca tctagtctga 3960 tttttcgtga cttgtatcgg ttttattgga ttcatccaac atatatcaat aaaaaatgag 4020 ttgtgtttcc tttcttccta gttcagttaa aattatttcc ctcctgcgct tgtgctgtaa 4080 ttgtctgtgt acctgttgtt tgtgactgtg ttagttccct tggatatgat ttcgtatttg 4140 atatgtacat ggagatagct tagcttcatt attggagtat gaagttagta tgacatagtc 4200 actctcctgg aaaattgaca ctgcaaacca tatttttatt ctgaaccaca aatcctagtc 4260 agtccgctgg catatgccgt ccgtttgctg aatccagaac gtgggtttgg agatgtacgg 4320 ctgagatgcc tctatgcgaa ggggatttcg tggtgaaacg agatgggagt agagcaacgc 4380 ccgtggaaga tgcttcaaac ttccacactt ttgagcaacg atcggcagta gtaaggtaga 4440 cgatttcaag atcaaagcat atgaagataa acaacatcaa caacaaaatt tgttggggtt 4500 ctatagagag aaacagagct acatacatac actgttttgt atctaccatc tgagatgatg 4560 aaaagatgaa aaactaaaga atgccccggc gccaacgcca ggacacgccg cgcgcgcgtc 4620 acccgagcca tctcttgacc cagccggcgc tgtatattta cacacgttgc agcatcgatc 4680 accacctgtt cgatcgcgtc gccgtcacca agcttggcgc gccggtcacc cggtccgggc 4740 ctagaaggcc acccggggct cggcgttagg cttttagtcc gctaggtggc tagaccgagg 4800 ttaggacgct agttcactag gggcctagtg gtgttttaag gatgggcaag tttgtacaaa 4860 aaagcaggct ggcgccggaa ccaattcagt cgagtgcggc cgccagtgtg atggatatct 4920 cgacccgggt gattgcggtt acatcatgta cggaaaaata attctaatcc ttgatttaaa 4980 tttgaacttg actatttatt tattctttat ttcattttgt aaatcatttt atgtatctcc 5040 tggcaagcaa ttttatccac cttgcaccaa caccttcggg ttccataatc aaaccacctt 5100 aacttcacac catgctgtaa ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa 5160 tttaataaac aatcatacga agcagtgaca aaataccaga tggtattaat gcttcgataa 5220 aattaattgg aaagtataaa atggtagaaa ataataaatt ataattaatt taagtaagat 5280 aaaaaataat taaaaactaa aatgttaaaa ttttaaaaaa attattttaa ataatattta 5340 aaaacattaa aaatcatttt aaaaaattta tttatagaac aattaaataa atatttcagc 5400 taataaaaaa caaaagctta cctagcctta gaagacaact tgtccaacaa ttagatgata 5460 cccattgccc ttacgttttc tttaacatca attattgttt ttgtcaacaa gctatctttt 5520 agttttattt tattggtaaa aaatatgtcg ccttcaagtt gcatcattta acacatctcg 5580 tcattagaaa aataaaactc ttccctaaac gattagtaga aaaaatcatt cgataataaa 5640 taagaaagaa aaattagaaa aaaataactt cattttaaaa aaatcattaa ggctatattt 5700 tttaaatgac taattttata tagactgtaa ctaaaagtat acaatttatt atgctatgta 5760 tcttaaagaa ttacttataa aaatctacgg aagaatatct tacaaagtga aaaacaaatg 5820 agaaagaatt tagtgggatg attatgattt tatttgaaaa ttgaaaaaat aattattaaa 5880 gactttagtg gagtaagaaa gctttcctat tagtcttttc ttatccataa aaaaaaaaaa 5940 aaaaatctag cgtgacagct tttccataga ttttaataat gtaaaatact ggtagcagcc 6000 gaccgttcag gtaatggaca ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat 6060 aacgccgtgg taacggataa agccaagcgt gaagcggtga aggtacatct ctgactccgt 6120 caagattacg aaaccgtcaa ctacgaagga ctccccgaaa tatcatctgt gtcataaaca 6180 ccaagtcaca ccatacatgg gcacgcgtca caatatgatt ggagaacggt tccaccgcat 6240 atgctataaa atgcccccac acccctcgac cctaatcgca cttcaattgc aatcaaatta 6300 gttcattctc tttgcgcagt tccctacctc tcctttcaag gttcgtagat ttcttccgtt 6360 tttttttctt cttctttatt gtttgttcta catcagcatg atgttgattt gattgtgttt 6420 tctatcgttt catcgattat aaattttcat aatcagaaga ttcagctttt attaatgcaa 6480 gaacgtcctt aattgatgat tttataaccg taaattaggt ctaattagag tttttttcat 6540 aaagattttc agatccgttt acaacaagcc ttaattgttg attctgtagt cgtagattaa 6600 ggtttttttc atgaactact tcagatccgt taaacaacag ccttatttgt tgatacttca 6660 gtcgtttttc aagaaattgt tcagatccgt tgataaaagc cttattcgtt gattctgtat 6720 ggtatttcaa gagatattgc tcaggtcctt tagcaactac cttatttgtt gattctgtgg 6780 ccatagatta ggattttttt tcacgaaatt gcttcttgaa attacgtgat ggattttgat 6840 tctgatttat cttgtgattg ttgactctac agccatggcc cacagcaagc acggcctgaa 6900 ggaggagatg accatgaagt accacatgga gggctgcgtg aacggccaca agttcgtgat 6960 caccggcgag ggcatcggct accccttcaa gggcaagcag accatcaacc tgtgcgtgat 7020 cgagggcggc cccctgccct tcagcgagga catcctgagc gccggcttca agtacggcga 7080 ccggatcttc accgagtacc cccaggacat cgtggactac ttcaagaaca gctgccccgc 7140 cggctacacc tggggccgga gcttcctgtt cgaggacggc gccgtgtgca tctgtaacgt 7200 ggacatcacc gtgagcgtga aggagaactg catctaccac aagagcatct tcaacggcgt 7260 gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc accaactggg aggccagctg 7320 cgagaagatc atgcccgtgc ctaagcaggg catcctgaag ggcgacgtga gcatgtacct 7380 gctgctgaag gacggcggcc ggtaccggtg ccagttcgac accgtgtaca aggccaagag 7440 cgtgcccagc aagatgcccg agtggcactt catccagcac aagctgctgc gggaggaccg 7500 gagcgacgcc aagaaccaga agtggcagct gaccgagcac gccatcgcct tccccagcgc 7560 cctggcctga gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa 7620 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta 7680 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta 7740 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg 7800 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattctag tggccggccc 7860 agctgatatc tagacccagc tttcttgtac aaagtggccg ttaacggatc ggccagaatg 7920 gcccggaccg ggttaccgaa ttcgagctcg gtaccctggg atccgatatc gatgggccct 7980 ggccgaagct tggtcacccg gtccgggcct agaaggccag cttcggccgc cccgggcaac 8040 tttattatac aaagttgatg gtacggatcc gtcgacggcg cgccgcggac ctgaattcca 8100 gcacactggc ggccgttagt ctaaaaacgg cccctaacta acgcttagtt gcccgctaga 8160 ttagtccccc gttagtgcct agttagacac acgacgaact aggggctact agtaacggcc 8220 gccagtgtgc tcggtacggc cagaatggcc atctggattc agcagggggt gattgcggtt 8280 acatcatgta cggaaaaata attctaatcc ttgatttaaa tttgaacttg actatttatt 8340 tattctttat ttcattttgt aaatcatttt atgtatctcc tggcaagcaa ttttatccac 8400 cttgcaccaa caccttcggg ttccataatc aaaccacctt aacttcacac catgctgtaa 8460 ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa tttaataaac aatcatacga 8520 agcagtgaca aaataccaga tggtattaat gcttcgataa aattaattgg aaagtataaa 8580 atggtagaaa ataataaatt ataattaatt taagtaagat aaaaaataat taaaaactaa 8640 aatgttaaaa ttttaaaaaa attattttaa ataatattta aaaacattaa aaatcatttt 8700 aaaaaattta tttatagaac aattaaataa atatttcagc taataaaaaa caaaagctta 8760 cctagcctta gaagacaact tgtccaacaa ttagatgata cccattgccc ttacgttttc 8820 tttaacatca attattgttt ttgtcaacaa gctatctttt agttttattt tattggtaaa 8880 aaatatgtcg ccttcaagtt gcatcattta acacatctcg tcattagaaa aataaaactc 8940 ttccctaaac gattagtaga aaaaatcatt cgataataaa taagaaagaa aaattagaaa 9000 aaaataactt cattttaaaa aaatcattaa ggctatattt tttaaatgac taattttata 9060 tagactgtaa ctaaaagtat acaatttatt atgctatgta tcttaaagaa ttacttataa 9120 aaatctacgg aagaatatct tacaaagtga aaaacaaatg agaaagaatt tagtgggatg 9180 attatgattt tatttgaaaa ttgaaaaaat aattattaaa gactttagtg gagtaagaaa 9240 gctttcctat tagtcttttc ttatccataa aaaaaaaaaa aaaaatctag cgtgacagct 9300 tttccataga ttttaataat gtaaaatact ggtagcagcc gaccgttcag gtaatggaca 9360 ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat aacgccgtgg taacggataa 9420 agccaagcgt gaagcggtga aggtacatct ctgactccgt caagattacg aaaccgtcaa 9480 ctacgaagga ctccccgaaa tatcatctgt gtcataaaca ccaagtcaca ccatacatgg 9540 gcacgcgtca caatatgatt ggagaacggt tccaccgcat atgctataaa atgcccccac 9600 acccctcgac cctaatcgca cttcaattgc aatcaaatta gttcattctc tttgcgcagt 9660 tccctacctc tcctttcaag gttcgtagat ttcttccgtt tttttttctt cttctttatt 9720 gtttgttcta catcagcatg atgttgattt gattgtgttt tctatcgttt catcgattat 9780 aaattttcat aatcagaaga ttcagctttt attaatgcaa gaacgtcctt aattgatgat 9840 tttataaccg taaattaggt ctaattagag tttttttcat aaagattttc agatccgttt 9900 acaacaagcc ttaattgttg attctgtagt cgtagattaa ggtttttttc atgaactact 9960 tcagatccgt taaacaacag ccttatttgt tgatacttca gtcgtttttc aagaaattgt 10020 tcagatccgt tgataaaagc cttattcgtt gattctgtat ggtatttcaa gagatattgc 10080 tcaggtcctt tagcaactac cttatttgtt gattctgtgg ccatagatta ggattttttt 10140 tcacgaaatt gcttcttgaa attacgtgat ggattttgat tctgatttat cttgtgattg 10200 ttgactctac agccatggct gcaactactc ttacatctgc tttacctggt gctttcagtt 10260 catcccagag accaagtgct cctttcaact tacaaagatc acctagagtg cttagaaggt 10320 tcaacagaaa gacaggacgt caacctcgtg gacttgttcg tgctgcaaag gctcaagcgg 10380 cgcaaaacct gccagagaaa ttcgatgaaa caagactctg ggaggccctc aggggatttg 10440 gggttagtcc aagtagggtt acttacgctc ccgtcggatt cggagattac cactggaccg 10500 tcacagatga agacgggaga ccttggttcg caactgtcag tgacctcgaa cacaaagagc 10560 attgcgggca gggagcacaa gcagccctta aaggactcag acaagctatg gacactgccc 10620 tcgcacttag agatagggac ggactcagat tcgtggttgc tccagtggca gcaacagatg 10680 ggggtggccc tgtccttccc cttgacgcta ggtatgcact cacagtcttc cctcacgtcc 10740 caggaagaac aggagagttc ggacaaagac tcacagaggc agagagggac agactgcttg 10800 ctcttctggc agaactccac ggaagaacac ctcctgaaac aaccccacca gccgatatgg 10860 aaccaccagg acttcctggt gttagagcag cactcgccga aagtgaagga ccttggagtg 10920 gaggaccatt tgcagaaccc gctagacttc ttctcagaga acacgaggcc acactgcacg 10980 caagactcgc tgaattcgaa gccctcgtcg caagagttaa gggtcgtgga gcacctcttg 11040 tcgttaccca cggagaacca cacccaggca acttgatcct cggagaggaa ggctatctcc 11100 tcgtggactg ggatacagtt ggactggctc cagctgaaag ggatttgagt ctcatctcag 11160 acgacccagc tgacctcgca agatacgcag agcttacagg aagaactcca gacccagacg 11220 cactcgcact ctatagactg cgttggagtc tccttgatgt ggctgagttc gtcgagtggt 11280 tcagggctga acaccaaaga acccaagaca ccgagagtgc atggaagggg ttcactgaca 11340 cacttgggca attggctgct ggtggatcag ctgcatagca aacttatctc tgttatgaat 11400 cagaagaagt tcatgtctcg tttcatttaa aactttggtg gtttgtgttt tggggccttg 11460 taaagcccct gatgaataat tgttcaacta tgtttccgtt cctgtgttat acctttcttt 11520 ctaatgagta atgacatcaa acttcttctg tattgaaatt atgtccttgt gagtctcttt 11580 atcatcgttt cgtctttaca ttatatgtgc tacttttgtc taatgagcct gaaaagtggc 11640 tccaatggta cgcactggaa gatttgttgg cttctggtag atatagcgac agtgttgagc 11700 ttgtaatatc atgtctctta ttgctaaatt agttcctttc ttaacagaaa ccttcaaagt 11760 ttttgttttt gttttcattt acctaatgta cacatacgct ggccatgact aacaacatgt 11820 ccaggcttag agcatatttt tttctagctt aaattgttaa cttgtcattc agtaaaatcc 11880 gagaattgtg aagctctaat tgaagctaat tcgttttata aagtcagtta aaaagtatac 11940 taaattatcc aacttttctt caaaatctca aaattctatg acaaaacgat agtctttgtt 12000 tatgtcagta ccacaaagag gtggaaaaaa acaccaaaaa aacaataagc aaactataca 12060 ctgagaagaa aaataaaaga gagctcaata gatgttttat actaacggta gattagatca 12120 aagatccaag ctttactcta catagagcag aacccagaat cccttcatat ctcttttatt 12180 ctagcaccga taatctactg aaaagaagac acttagagct ctgtctcttt gtcaaagaag 12240 tcccagccgt catccagaag ctccttacgt tcattaacag aggccgcaat tggatcgggt 12300 ttacttattt tgtgggtatc tatactttta ttagattttt aatcaggctc ctgatttctt 12360 tttatttcga ttgaattcct gaacttgtat tattcagtag atcgaataaa ttataaaaag 12420 ataaaatcat aaaataatat tttatcctat caatcatatt aaagcaatga atatgtaaaa 12480 ttaatcttat ctttatttta aaaaatcata taggtttagt atttttttaa aaataaagat 12540 aggattagtt ttactattca ctgcttatta cttttaaaaa aatcataaag gtttagtatt 12600 tttttaaaat aaatatagga atagttttac tattcactgc tttaatagaa aaatagttta 12660 aaatttaaga tagttttaat cccagcattt gccacgtttg aacgtgagcc gaaacgatgt 12720 cgttacatta tcttaaccta gctgaaacga tgtcgtcata atatcgccaa atgccaactg 12780 gactacgtcg aacccacaaa tcccacaaag cgcgtgaaat caaatcgctc aaaccacaaa 12840 aaagaacaac gcgtttgtta cacgctcaat cccacgcgag tagagcacag taaccttcaa 12900 ataagcgaat ggggcataat cagaaatccg aaataaacct aggggcatta tcggaaatga 12960 aaagtagctc actcaatata aaaatctagg aaccctagtt ttcgttatca ctctgtgctc 13020 cctcgctcta tttctcagtc tctgtgtttg cggctgagga ttccgaacga gtgaccttct 13080 tcgtttctcg caaaggtaac agcctctgct cttgtctctt cgattcgatc tatgcctgtc 13140 tcttatttac gatgatgttt cttcggttat gtttttttat ttatgcttta tgctgttgat 13200 gttcggttgt ttgtttcgct ttgtttttgt ggttcagttt tttaggattc ttttggtttt 13260 tgaatcgatt aatcggaaga gattttcgag ttatttggtg tgttggaggt gaatcttttt 13320 tttgaggtca tagatctgtt gtatttgtgt tataaacatg cgactttgta tgatttttta 13380 cgaggttatg atgttctggt tgttttatta tgaatctgtt gagacagaac catgattttt 13440 gttgatgttc gtttacacta ttaaaggttt gttttaacag gattaaaagt tttttaagca 13500 tgttgaagga gtcttgtaga tatgtaaccg tcgatagttt ttttgtgggt ttgttcacat 13560 gttatcaagc ttaatctttt actatgtatg cgaccatatc tggatccagc aaaggcgatt 13620 ttttaattcc ttgtgaaact tttgtaatat gaagttgaaa ttttgttatt ggtaaactat 13680 aaatgtgtga agttggagta tacctttacc ttcttatttg gctttgtgat agtttaattt 13740 atatgtattt tgagttctga cttgtatttc tttgaattga ttctagttta agtaatcatg 13800 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc 13860 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac 13920 aacaccgtga agctgaaggt gacgaagggc ggccccctgc ccttcgcctg ggacatcctg 13980 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac 14040 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 14100 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag 14160 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg 14220 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc 14280 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac 14340 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc 14400 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac 14460 cacctgttcc tgtagttttg tgatctgatg ataagtggtt ggttcgtgtc tcatgcactt 14520 gggaggtgat ctatttcacc tggtgtagtt tgtgtttccg tcagttggaa aaacttatcc 14580 ctatcgattt cgttttcatt ttctgctttt cttttatgta ccttcgtttg ggcttgtaac 14640 gggcctttgt atttcaactc tcaataataa tccaagtgca tgttaaacaa tttgtcatct 14700 gtttcggctt tgatatacta ctggtgaaga tgggccgtac tactgcatca caacgaaaaa 14760 taataataag atgaaaaact tgaagtggaa aaaaaaaaaa acttgaatgt tcactactac 14820 tcattgacca taatgtttaa catacatagc tcaatagtat ttttgtgaat atggcaacac 14880 aaacagtcca aaacaattgt ctcttactat accaaaccaa gggcgccgct tgtttgccac 14940 tctttgtgtg caatagtgtg attaccacat ctccacattc aatatattcc ctgaattatc 15000 tgacgatttt gatggctcac tgttttccca agtcttgaat tgtcttctgt gcgccagtca 15060 aatgcatatg tgttgagttt atcttttaaa tatcaagctt ttgtttttaa cttttgtttg 15120 taaccaaaaa ctcacagtag gagtttgatc acataatttt atgtttgcct ttgcaatttc 15180 tagtgagtct ttgattaaaa gcttgaaaag aaaatgcagc caagcttacc aagtaagtta 15240 tgtgtattaa ccagaggaag agagaatctt gcaaaatttc aacaaacaca aaaagaagta 15300 ttactacgat tggtggagaa agaaaacgat tccaaatctt gaactgttgt tgtaaaagca 15360 tagcagaaag tgggagacaa ccgaaataga aatgactata acttaattta atgttatcat 15420 tataatttct tctagcaaat atttagaaag taaatatcac atcaaccttt aatgtaatta 15480 agctttctct ttttgattca tgtgagatga aaagaaaaaa aagaagagaa aagtgtagaa 15540 aacacatcat ttctaagctg aagctgaggt acaaattaat taataagtga ctagggtcac 15600 gtgaccctag tcacttatga ttccgatgac ttcgtaggtt cctagctcaa gccgctcgtg 15660 tccaagcgtc acttacgatt agctaatgat tacggcatct aggaccgact agttcttatg 15720 tgcttctagt ctccaaatgt ggttgatagt tattttgctc taagatcaac agtaatgaag 15780 tataaatcat cgttgtggtg tgctactcgg ttaattgagc attaacacac acaaacatga 15840 cgaggatggt ataatctcca aaaatgtgta ctttgttagg tgggacccta tagccttgat 15900 taatgtgcta tgttaggcat gcctggaaac gtgtgacgca tatgttttgt gaacctgttg 15960 atattatatg tgcttttata ttaccatatt ttattaaaat actaatattt attactagta 16020 agatataaca ttctatctag cttaaaaact aaccataaat attccataat aactagattt 16080 accaaactaa tatactaaat atacataata aatacaaaat taacaagaca ataatcaata 16140 tttatgagct taatatattt agacattatg gttggtcgac gataatcatg ctaacttttc 16200 gtaattgctt gattgaaata tgcttagaat aatgcctctt tgttctacat ggcaaatagg 16260 gaccattatg gtgtaacacc ctgggaacca caaacacccc gaaatgctac taaactacac 16320 aactaacctt catatataaa atttcgacag catctccttt gaaaatttgc atagacgtgg 16380 aagcaacaga gtataaacag atctagctag ctaagtttaa acggccggcc aaacgtgcaa 16440 gcgctactag acaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag 16500 cgtcaattta tttacaattg aatatatcct gcca 16534 <210> 170 <211> 13210 <212> DNA <213> Artificial Sequence <220> <223> RV026532 - T-DNA <400> 170 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccgaattcga gctcggtacg gccagaatgg cccggaccgg gttaccgaat 1440 tcgagctcgg taccctggga tccgatatcg atgggccctg gccgaagctt ggtcacccgg 1500 tccgggccta gaaggccagc ttcaagtttg tacaaaaaag caggctggcg ccggaaccaa 1560 ttcagtcgag tgcggccgcc agtgtgatgg atatctcgac ccgggtgatt gcggttacat 1620 catgtacgga aaaataattc taatccttga tttaaatttg aacttgacta tttatttatt 1680 ctttatttca ttttgtaaat cattttatgt atctcctggc aagcaatttt atccaccttg 1740 caccaacacc ttcgggttcc ataatcaaac caccttaact tcacaccatg ctgtaactca 1800 caccgcccag catctccaat gtgaaagaag ctaaaattta ataaacaatc atacgaagca 1860 gtgacaaaat accagatggt attaatgctt cgataaaatt aattggaaag tataaaatgg 1920 tagaaaataa taaattataa ttaatttaag taagataaaa aataattaaa aactaaaatg 1980 ttaaaatttt aaaaaaatta ttttaaataa tatttaaaaa cattaaaaat cattttaaaa 2040 aatttattta tagaacaatt aaataaatat ttcagctaat aaaaaacaaa agcttaccta 2100 gccttagaag acaacttgtc caacaattag atgataccca ttgcccttac gttttcttta 2160 acatcaatta ttgtttttgt caacaagcta tcttttagtt ttattttatt ggtaaaaaat 2220 atgtcgcctt caagttgcat catttaacac atctcgtcat tagaaaaata aaactcttcc 2280 ctaaacgatt agtagaaaaa atcattcgat aataaataag aaagaaaaat tagaaaaaaa 2340 taacttcatt ttaaaaaaat cattaaggct atatttttta aatgactaat tttatataga 2400 ctgtaactaa aagtatacaa tttattatgc tatgtatctt aaagaattac ttataaaaat 2460 ctacggaaga atatcttaca aagtgaaaaa caaatgagaa agaatttagt gggatgatta 2520 tgattttatt tgaaaattga aaaaataatt attaaagact ttagtggagt aagaaagctt 2580 tcctattagt cttttcttat ccataaaaaa aaaaaaaaaa atctagcgtg acagcttttc 2640 catagatttt aataatgtaa aatactggta gcagccgacc gttcaggtaa tggacactgt 2700 ggtcctaact tgcaacgggt gcgggcccaa tttaataacg ccgtggtaac ggataaagcc 2760 aagcgtgaag cggtgaaggt acatctctga ctccgtcaag attacgaaac cgtcaactac 2820 gaaggactcc ccgaaatatc atctgtgtca taaacaccaa gtcacaccat acatgggcac 2880 gcgtcacaat atgattggag aacggttcca ccgcatatgc tataaaatgc ccccacaccc 2940 ctcgacccta atcgcacttc aattgcaatc aaattagttc attctctttg cgcagttccc 3000 tacctctcct ttcaaggttc gtagatttct tccgtttttt tttcttcttc tttattgttt 3060 gttctacatc agcatgatgt tgatttgatt gtgttttcta tcgtttcatc gattataaat 3120 tttcataatc agaagattca gcttttatta atgcaagaac gtccttaatt gatgatttta 3180 taaccgtaaa ttaggtctaa ttagagtttt tttcataaag attttcagat ccgtttacaa 3240 caagccttaa ttgttgattc tgtagtcgta gattaaggtt tttttcatga actacttcag 3300 atccgttaaa caacagcctt atttgttgat acttcagtcg tttttcaaga aattgttcag 3360 atccgttgat aaaagcctta ttcgttgatt ctgtatggta tttcaagaga tattgctcag 3420 gtcctttagc aactacctta tttgttgatt ctgtggccat agattaggat tttttttcac 3480 gaaattgctt cttgaaatta cgtgatggat tttgattctg atttatcttg tgattgttga 3540 ctctacagcc atggcccaca gcaagcacgg cctgaaggag gagatgacca tgaagtacca 3600 catggagggc tgcgtgaacg gccacaagtt cgtgatcacc ggcgagggca tcggctaccc 3660 cttcaagggc aagcagacca tcaacctgtg cgtgatcgag ggcggccccc tgcccttcag 3720 cgaggacatc ctgagcgccg gcttcaagta cggcgaccgg atcttcaccg agtaccccca 3780 ggacatcgtg gactacttca agaacagctg ccccgccggc tacacctggg gccggagctt 3840 cctgttcgag gacggcgccg tgtgcatctg taacgtggac atcaccgtga gcgtgaagga 3900 gaactgcatc taccacaaga gcatcttcaa cggcgtgaac ttccccgccg acggccccgt 3960 gatgaagaag atgaccacca actgggaggc cagctgcgag aagatcatgc ccgtgcctaa 4020 gcagggcatc ctgaagggcg acgtgagcat gtacctgctg ctgaaggacg gcggccggta 4080 ccggtgccag ttcgacaccg tgtacaaggc caagagcgtg cccagcaaga tgcccgagtg 4140 gcacttcatc cagcacaagc tgctgcggga ggaccggagc gacgccaaga accagaagtg 4200 gcagctgacc gagcacgcca tcgccttccc cagcgccctg gcctgagagc tcgaatttcc 4260 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg 4320 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat 4380 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat 4440 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat 4500 ctatgttact agatcgggaa ttctagtggc cggcccagct gatatctaga cccagctttc 4560 ttgtacaaag tggccgttaa cggatcggcc agaatggccc ggaccgggtt accgaattcg 4620 agctcggtac cctgggatcc gatatcgatg ggccctggcc gaagcttggt cacccggtcc 4680 gggcctagaa ggccagcttc ggccgccccg ggcaacttta ttatacaaag ttgatggtac 4740 ggatccgtcg acggcgcgcc gcggacctga attccagcac actggcggcc gttagtctaa 4800 aaacggcccc taactaacgc ttagttgccc gctagattag tcccccgtta gtgcctagtt 4860 agacacacga cgaactaggg gctactagta acggccgcca gtgtgctcgg tacggccaga 4920 atggccatct ggattcagca gggggtgatt gcggttacat catgtacgga aaaataattc 4980 taatccttga tttaaatttg aacttgacta tttatttatt ctttatttca ttttgtaaat 5040 cattttatgt atctcctggc aagcaatttt atccaccttg caccaacacc ttcgggttcc 5100 ataatcaaac caccttaact tcacaccatg ctgtaactca caccgcccag catctccaat 5160 gtgaaagaag ctaaaattta ataaacaatc atacgaagca gtgacaaaat accagatggt 5220 attaatgctt cgataaaatt aattggaaag tataaaatgg tagaaaataa taaattataa 5280 ttaatttaag taagataaaa aataattaaa aactaaaatg ttaaaatttt aaaaaaatta 5340 ttttaaataa tatttaaaaa cattaaaaat cattttaaaa aatttattta tagaacaatt 5400 aaataaatat ttcagctaat aaaaaacaaa agcttaccta gccttagaag acaacttgtc 5460 caacaattag atgataccca ttgcccttac gttttcttta acatcaatta ttgtttttgt 5520 caacaagcta tcttttagtt ttattttatt ggtaaaaaat atgtcgcctt caagttgcat 5580 catttaacac atctcgtcat tagaaaaata aaactcttcc ctaaacgatt agtagaaaaa 5640 atcattcgat aataaataag aaagaaaaat tagaaaaaaa taacttcatt ttaaaaaaat 5700 cattaaggct atatttttta aatgactaat tttatataga ctgtaactaa aagtatacaa 5760 tttattatgc tatgtatctt aaagaattac ttataaaaat ctacggaaga atatcttaca 5820 aagtgaaaaa caaatgagaa agaatttagt gggatgatta tgattttatt tgaaaattga 5880 aaaaataatt attaaagact ttagtggagt aagaaagctt tcctattagt cttttcttat 5940 ccataaaaaa aaaaaaaaaa atctagcgtg acagcttttc catagatttt aataatgtaa 6000 aatactggta gcagccgacc gttcaggtaa tggacactgt ggtcctaact tgcaacgggt 6060 gcgggcccaa tttaataacg ccgtggtaac ggataaagcc aagcgtgaag cggtgaaggt 6120 acatctctga ctccgtcaag attacgaaac cgtcaactac gaaggactcc ccgaaatatc 6180 atctgtgtca taaacaccaa gtcacaccat acatgggcac gcgtcacaat atgattggag 6240 aacggttcca ccgcatatgc tataaaatgc ccccacaccc ctcgacccta atcgcacttc 6300 aattgcaatc aaattagttc attctctttg cgcagttccc tacctctcct ttcaaggttc 6360 gtagatttct tccgtttttt tttcttcttc tttattgttt gttctacatc agcatgatgt 6420 tgatttgatt gtgttttcta tcgtttcatc gattataaat tttcataatc agaagattca 6480 gcttttatta atgcaagaac gtccttaatt gatgatttta taaccgtaaa ttaggtctaa 6540 ttagagtttt tttcataaag attttcagat ccgtttacaa caagccttaa ttgttgattc 6600 tgtagtcgta gattaaggtt tttttcatga actacttcag atccgttaaa caacagcctt 6660 atttgttgat acttcagtcg tttttcaaga aattgttcag atccgttgat aaaagcctta 6720 ttcgttgatt ctgtatggta tttcaagaga tattgctcag gtcctttagc aactacctta 6780 tttgttgatt ctgtggccat agattaggat tttttttcac gaaattgctt cttgaaatta 6840 cgtgatggat tttgattctg atttatcttg tgattgttga ctctacagcc atggctgcaa 6900 ctactcttac atctgcttta cctggtgctt tcagttcatc ccagagacca agtgctcctt 6960 tcaacttaca aagatcacct agagtgctta gaaggttcaa cagaaagaca ggacgtcaac 7020 ctcgtggact tgttcgtgct gcaaaggctc aagcggcgca aaacctgcca gagaaattcg 7080 atgaaacaag actctgggag gccctcaggg gatttggggt tagtccaagt agggttactt 7140 acgctcccgt cggattcgga gattaccact ggaccgtcac agatgaagac gggagacctt 7200 ggttcgcaac tgtcagtgac ctcgaacaca aagagcattg cgggcaggga gcacaagcag 7260 cccttaaagg actcagacaa gctatggaca ctgccctcgc acttagagat agggacggac 7320 tcagattcgt ggttgctcca gtggcagcaa cagatggggg tggccctgtc cttccccttg 7380 acgctaggta tgcactcaca gtcttccctc acgtcccagg aagaacagga gagttcggac 7440 aaagactcac agaggcagag agggacagac tgcttgctct tctggcagaa ctccacggaa 7500 gaacacctcc tgaaacaacc ccaccagccg atatggaacc accaggactt cctggtgtta 7560 gagcagcact cgccgaaagt gaaggacctt ggagtggagg accatttgca gaacccgcta 7620 gacttcttct cagagaacac gaggccacac tgcacgcaag actcgctgaa ttcgaagccc 7680 tcgtcgcaag agttaagggt cgtggagcac ctcttgtcgt tacccacgga gaaccacacc 7740 caggcaactt gatcctcgga gaggaaggct atctcctcgt ggactgggat acagttggac 7800 tggctccagc tgaaagggat ttgagtctca tctcagacga cccagctgac ctcgcaagat 7860 acgcagagct tacaggaaga actccagacc cagacgcact cgcactctat agactgcgtt 7920 ggagtctcct tgatgtggct gagttcgtcg agtggttcag ggctgaacac caaagaaccc 7980 aagacaccga gagtgcatgg aaggggttca ctgacacact tgggcaattg gctgctggtg 8040 gatcagctgc atagcaaact tatctctgtt atgaatcaga agaagttcat gtctcgtttc 8100 atttaaaact ttggtggttt gtgttttggg gccttgtaaa gcccctgatg aataattgtt 8160 caactatgtt tccgttcctg tgttatacct ttctttctaa tgagtaatga catcaaactt 8220 cttctgtatt gaaattatgt ccttgtgagt ctctttatca tcgtttcgtc tttacattat 8280 atgtgctact tttgtctaat gagcctgaaa agtggctcca atggtacgca ctggaagatt 8340 tgttggcttc tggtagatat agcgacagtg ttgagcttgt aatatcatgt ctcttattgc 8400 taaattagtt cctttcttaa cagaaacctt caaagttttt gtttttgttt tcatttacct 8460 aatgtacaca tacgctggcc atgactaaca acatgtccag gcttagagca tatttttttc 8520 tagcttaaat tgttaacttg tcattcagta aaatccgaga attgtgaagc tctaattgaa 8580 gctaattcgt tttataaagt cagttaaaaa gtatactaaa ttatccaact tttcttcaaa 8640 atctcaaaat tctatgacaa aacgatagtc tttgtttatg tcagtaccac aaagaggtgg 8700 aaaaaaacac caaaaaaaca ataagcaaac tatacactga gaagaaaaat aaaagagagc 8760 tcaatagatg ttttatacta acggtagatt agatcaaaga tccaagcttt actctacata 8820 gagcagaacc cagaatccct tcatatctct tttattctag caccgataat ctactgaaaa 8880 gaagacactt agagctctgt ctctttgtca aagaagtccc agccgtcatc cagaagctcc 8940 ttacgttcat taacagaggc cgcaattgga tcgggtttac ttattttgtg ggtatctata 9000 cttttattag atttttaatc aggctcctga tttcttttta tttcgattga attcctgaac 9060 ttgtattatt cagtagatcg aataaattat aaaaagataa aatcataaaa taatatttta 9120 tcctatcaat catattaaag caatgaatat gtaaaattaa tcttatcttt attttaaaaa 9180 atcatatagg tttagtattt ttttaaaaat aaagatagga ttagttttac tattcactgc 9240 ttattacttt taaaaaaatc ataaaggttt agtatttttt taaaataaat ataggaatag 9300 ttttactatt cactgcttta atagaaaaat agtttaaaat ttaagatagt tttaatccca 9360 gcatttgcca cgtttgaacg tgagccgaaa cgatgtcgtt acattatctt aacctagctg 9420 aaacgatgtc gtcataatat cgccaaatgc caactggact acgtcgaacc cacaaatccc 9480 acaaagcgcg tgaaatcaaa tcgctcaaac cacaaaaaag aacaacgcgt ttgttacacg 9540 ctcaatccca cgcgagtaga gcacagtaac cttcaaataa gcgaatgggg cataatcaga 9600 aatccgaaat aaacctaggg gcattatcgg aaatgaaaag tagctcactc aatataaaaa 9660 tctaggaacc ctagttttcg ttatcactct gtgctccctc gctctatttc tcagtctctg 9720 tgtttgcggc tgaggattcc gaacgagtga ccttcttcgt ttctcgcaaa ggtaacagcc 9780 tctgctcttg tctcttcgat tcgatctatg cctgtctctt atttacgatg atgtttcttc 9840 ggttatgttt ttttatttat gctttatgct gttgatgttc ggttgtttgt ttcgctttgt 9900 ttttgtggtt cagtttttta ggattctttt ggtttttgaa tcgattaatc ggaagagatt 9960 ttcgagttat ttggtgtgtt ggaggtgaat cttttttttg aggtcataga tctgttgtat 10020 ttgtgttata aacatgcgac tttgtatgat tttttacgag gttatgatgt tctggttgtt 10080 ttattatgaa tctgttgaga cagaaccatg atttttgttg atgttcgttt acactattaa 10140 aggtttgttt taacaggatt aaaagttttt taagcatgtt gaaggagtct tgtagatatg 10200 taaccgtcga tagttttttt gtgggtttgt tcacatgtta tcaagcttaa tcttttacta 10260 tgtatgcgac catatctgga tccagcaaag gcgatttttt aattccttgt gaaacttttg 10320 taatatgaag ttgaaatttt gttattggta aactataaat gtgtgaagtt ggagtatacc 10380 tttaccttct tatttggctt tgtgatagtt taatttatat gtattttgag ttctgacttg 10440 tatttctttg aattgattct agtttaagta atcatggcct cctccgagaa cgtcatcacc 10500 gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc 10560 gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacg 10620 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc 10680 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag 10740 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag 10800 gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc 10860 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc 10920 ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac 10980 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg 11040 cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc 11100 atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta gttttgtgat 11160 ctgatgataa gtggttggtt cgtgtctcat gcacttggga ggtgatctat ttcacctggt 11220 gtagtttgtg tttccgtcag ttggaaaaac ttatccctat cgatttcgtt ttcattttct 11280 gcttttcttt tatgtacctt cgtttgggct tgtaacgggc ctttgtattt caactctcaa 11340 taataatcca agtgcatgtt aaacaatttg tcatctgttt cggctttgat atactactgg 11400 tgaagatggg ccgtactact gcatcacaac gaaaaataat aataagatga aaaacttgaa 11460 gtggaaaaaa aaaaaaactt gaatgttcac tactactcat tgaccataat gtttaacata 11520 catagctcaa tagtattttt gtgaatatgg caacacaaac agtccaaaac aattgtctct 11580 tactatacca aaccaagggc gccgcttgtt tgccactctt tgtgtgcaat agtgtgatta 11640 ccacatctcc acattcaata tattccctga attatctgac gattttgatg gctcactgtt 11700 ttcccaagtc ttgaattgtc ttctgtgcgc cagtcaaatg catatgtgtt gagtttatct 11760 tttaaatatc aagcttttgt ttttaacttt tgtttgtaac caaaaactca cagtaggagt 11820 ttgatcacat aattttatgt ttgcctttgc aatttctagt gagtctttga ttaaaagctt 11880 gaaaagaaaa tgcagccaag cttaccaagt aagttatgtg tattaaccag aggaagagag 11940 aatcttgcaa aatttcaaca aacacaaaaa gaagtattac tacgattggt ggagaaagaa 12000 aacgattcca aatcttgaac tgttgttgta aaagcatagc agaaagtggg agacaaccga 12060 aatagaaatg actataactt aatttaatgt tatcattata atttcttcta gcaaatattt 12120 agaaagtaaa tatcacatca acctttaatg taattaagct ttctcttttt gattcatgtg 12180 agatgaaaag aaaaaaaaga agagaaaagt gtagaaaaca catcatttct aagctgaagc 12240 tgaggtacaa attaattaat aagtgactag ggtcacgtga ccctagtcac ttatgattcc 12300 gatgacttcg taggttccta gctcaagccg ctcgtgtcca agcgtcactt acgattagct 12360 aatgattacg gcatctagga ccgactagtt cttatgtgct tctagtctcc aaatgtggtt 12420 gatagttatt ttgctctaag atcaacagta atgaagtata aatcatcgtt gtggtgtgct 12480 actcggttaa ttgagcatta acacacacaa acatgacgag gatggtataa tctccaaaaa 12540 tgtgtacttt gttaggtggg accctatagc cttgattaat gtgctatgtt aggcatgcct 12600 ggaaacgtgt gacgcatatg ttttgtgaac ctgttgatat tatatgtgct tttatattac 12660 catattttat taaaatacta atatttatta ctagtaagat ataacattct atctagctta 12720 aaaactaacc ataaatattc cataataact agatttacca aactaatata ctaaatatac 12780 ataataaata caaaattaac aagacaataa tcaatattta tgagcttaat atatttagac 12840 attatggttg gtcgacgata atcatgctaa cttttcgtaa ttgcttgatt gaaatatgct 12900 tagaataatg cctctttgtt ctacatggca aatagggacc attatggtgt aacaccctgg 12960 gaaccacaaa caccccgaaa tgctactaaa ctacacaact aaccttcata tataaaattt 13020 cgacagcatc tcctttgaaa atttgcatag acgtggaagc aacagagtat aaacagatct 13080 agctagctaa gtttaaacgg ccggccaaac gtgcaagcgc tactagacaa ttcagtacat 13140 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttattta caattgaata 13200 tatcctgcca 13210 <210> 171 <211> 16939 <212> DNA <213> Artificial Sequence <220> <223> RV027337 T-DNA <400> 171 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgctttga gccaacagtc 1440 ggaaaacaac catcacttag cattttttta tttttaatac tactattttt ttttttgtaa 1500 ttagtcgagt gtaccaatcc caaatttaat gggtatcttt cataaccaaa aagaaaaaag 1560 cacaaaaaca aaatactact actatactat aatggtgaaa cacacattac attagcatta 1620 accgctattc cttaaaagat atatgagcag actacaagga agtacattta aatcagtgtg 1680 ctaagttttc tcaattatca atctaataat tgtacttaca taaatttacc aaatcatctc 1740 accatttagg gcatacaatt tttgctcaat aatggagctt gaaaagacta attatagaat 1800 gtctttttgg ttgacacaag ctcgcttctt catgtcgttt actttaaaaa ttgtagattc 1860 tttcacaact tataagattc tatagcctat ttgattatcc tttaggatga tgagtataat 1920 atctcaaact ataaaataaa aatgttcaca tcaataattg aaaaaaaatg catgtatgaa 1980 ttcacgaaaa tatggttacg gttgtgtgtt gctgctagac aaaagtcttt catatattca 2040 gttatatttc ttaaaactaa tcaaaattgt tttatgcaat tttggcgagt agagagaaaa 2100 agcgcgtggg ataaataaag aaaggcgtta taaaaaaaga gaaaaagtca accgagtagt 2160 tctggtaaga gagtctcgtg cattcgtaag gtttccaaga gtccacatat ctatttcatc 2220 atcctcttaa cgtttttctc acttactttt ttccccactc ttaattgatt ccgttgataa 2280 aatttgttcg ttatatagaa ataatcagaa tataattagg gttaattttg gagtaaagac 2340 cattcaatca atggtttggc ttgattcttg tttgaaattc tatgtttaaa aagggtaata 2400 cttacaaact tgactttatt agtattttaa aaaatgacaa aacttaaaat ggatttgaaa 2460 tctagcatat ttaactttaa tacagttttg gtttaagttt cactctcttt tctatcaaaa 2520 atataatttt aaaatttgaa agatgttaaa atagacaaat gtcattagtt tgttcagatt 2580 tagttatgtt tttaatatac agacaattat attaaaataa taataaaaaa gtcagatcag 2640 ttataaaaga aagtcagttc agttttaaga cttaagtttg agtcttttgg atttgaattt 2700 aatagattca cttttattaa aaattgttga aacacttttt aaaaactaaa acatttgtca 2760 aaaaatgcat ttaaccaaaa aaatgaaaat gaaaggtagt aagtaataaa agaagaaaac 2820 aaaatctaaa aaagtgtctt taaaatctca aaattaaaat atacaaaaaa gtacaagaaa 2880 ataaaagaaa agggggaaaa agagagtcaa tatcaacatg caagtgtgaa tattagactc 2940 ttcttctctc atcagttacc ccaaaactag ggtttaccca ttttcatcat cttcttcttc 3000 cccacatcac tctctgaaag acaataagag agagagacac acacaccaaa aaaaaaaccc 3060 cattgatttt taagagagag agagagactg agaacgaaga gcaaatgaga gagaagttga 3120 atcaaatttc cgctttcgtt ctctttctct ctcctccatt tcagtaataa acactccttc 3180 ctctttaatt tttctgggtt tcttcttctt ccctcttttg ttgtttctgt gtgattcagt 3240 tctcgatcca tcttttcgtg aaaagtatca atcttttaat tctctagttg tttgtcaaat 3300 ctggttagtt tcttctcttg tatttatttg ccatggaaac tcaacaacaa caagcagata 3360 ttcaagattt tgggaacaaa aacagtaaca cctatgcatg caggcaaagc agtactaggt 3420 ggacacccac aagtgatcag atcagaatac tgaaagagct ctactacaac aatggaatca 3480 gatcaccaac tgctgatcag attcagagaa tcgctgctca actcagacag tacggcaaga 3540 ttgaaggcaa gaacgtgttt tattggtttc agaaccataa agctcgtgaa cgccagaaga 3600 aacggttcac tcctactgct cctccaccac cgtctcactt ctccgatcat attaaccacc 3660 atcttcccaa cgctgccccc atgcagattc cgtctcacca ccaccactac caccatcaag 3720 aaccaccaca tgtctatgct caccctcaca aactctacac cactcatcac attggggttg 3780 gttcttcttc acaaggagtg atgggtgtag ggtgtggcta tggatctgtt gctatggaga 3840 agagtttcag gaagtgttca atatcaccac cgggagagag taaggccaca ggaggaattg 3900 gccggaatat cggttcaaga tcacggataa gtgtcgattc gtgttccttc ttcgacacaa 3960 ttaagccaaa gacatatgag atgtttgaga atcatgatca agatgaagaa caaggagagc 4020 cttcaactga gatcgaaaca cttccattat tcccgattca cgacggcaac catcatgatt 4080 tcttcggcat gaggacggca aatctgcaat tggagcaagg cactggaggt tactacactg 4140 gaggaagctg gtatcggtct gatgagaggg cgtctcttga gctcagtctc aactcatatg 4200 gttattataa ctaattcgaa ctggccaggt acctgacatc ttatagtctg caacctctcg 4260 tgtctgaatt cctatcttta tcaagtgtta ttgcttccac gactatagga cagctttcgt 4320 cgaaaggttt tgctcatgtg atctcgaagg attcatctag tctgattttt cgtgacttgt 4380 atcggtttta ttggattcat ccaacatata tcaataaaaa atgagttgtg tttcctttct 4440 tcctagttca gttaaaatta tttccctcct gcgcttgtgc tgtaattgtc tgtgtacctg 4500 ttgtttgtga ctgtgttagt tcccttggat atgatttcgt atttgatatg tacatggaga 4560 tagcttagct tcattattgg agtatgaagt tagtatgaca tagtcactct cctggaaaat 4620 tgacactgca aaccatattt ttattctgaa ccacaaatcc tagtcagtcc gctggcatat 4680 gccgtccgtt tgctgaatcc agaacgtggg tttggagatg tacggctgag atgcctctat 4740 gcgaagggga tttcgtggtg aaacgagatg ggagtagagc aacgcccgtg gaagatgctt 4800 caaacttcca cacttttgag caacgatcgg cagtagtaag gtagacgatt tcaagatcaa 4860 agcatatgaa gataaacaac atcaacaaca aaatttgttg gggttctata gagagaaaca 4920 gagctacata catacactgt tttgtatcta ccatctgaga tgatgaaaag atgaaaaact 4980 aaagaatgcc ccggcgccaa cgccaggaca cgccgcgcgc gcgtcacccg agccatctct 5040 tgacccagcc ggcgctgtat atttacacac gttgcagcat cgatcaccac ctgttcgatc 5100 gcgtcgccgt caccaagctt ggcgcgccgg tcacccggtc cgggcctaga aggccacccg 5160 gggctcggcg ttaggctttt agtccgctag gtggctagac cgaggttagg acgctagttc 5220 actaggggcc tagtggtgtt ttaaggatgg gcaagtttgt acaaaaaagc aggctggcgc 5280 cggaaccaat tcagtcgagt gcggccgcca gtgtgatgga tatctcgacc cgggtgattg 5340 cggttacatc atgtacggaa aaataattct aatccttgat ttaaatttga acttgactat 5400 ttatttattc tttatttcat tttgtaaatc attttatgta tctcctggca agcaatttta 5460 tccaccttgc accaacacct tcgggttcca taatcaaacc accttaactt cacaccatgc 5520 tgtaactcac accgcccagc atctccaatg tgaaagaagc taaaatttaa taaacaatca 5580 tacgaagcag tgacaaaata ccagatggta ttaatgcttc gataaaatta attggaaagt 5640 ataaaatggt agaaaataat aaattataat taatttaagt aagataaaaa ataattaaaa 5700 actaaaatgt taaaatttta aaaaaattat tttaaataat atttaaaaac attaaaaatc 5760 attttaaaaa atttatttat agaacaatta aataaatatt tcagctaata aaaaacaaaa 5820 gcttacctag ccttagaaga caacttgtcc aacaattaga tgatacccat tgcccttacg 5880 ttttctttaa catcaattat tgtttttgtc aacaagctat cttttagttt tattttattg 5940 gtaaaaaata tgtcgccttc aagttgcatc atttaacaca tctcgtcatt agaaaaataa 6000 aactcttccc taaacgatta gtagaaaaaa tcattcgata ataaataaga aagaaaaatt 6060 agaaaaaaat aacttcattt taaaaaaatc attaaggcta tattttttaa atgactaatt 6120 ttatatagac tgtaactaaa agtatacaat ttattatgct atgtatctta aagaattact 6180 tataaaaatc tacggaagaa tatcttacaa agtgaaaaac aaatgagaaa gaatttagtg 6240 ggatgattat gattttattt gaaaattgaa aaaataatta ttaaagactt tagtggagta 6300 agaaagcttt cctattagtc ttttcttatc cataaaaaaa aaaaaaaaaa tctagcgtga 6360 cagcttttcc atagatttta ataatgtaaa atactggtag cagccgaccg ttcaggtaat 6420 ggacactgtg gtcctaactt gcaacgggtg cgggcccaat ttaataacgc cgtggtaacg 6480 gataaagcca agcgtgaagc ggtgaaggta catctctgac tccgtcaaga ttacgaaacc 6540 gtcaactacg aaggactccc cgaaatatca tctgtgtcat aaacaccaag tcacaccata 6600 catgggcacg cgtcacaata tgattggaga acggttccac cgcatatgct ataaaatgcc 6660 cccacacccc tcgaccctaa tcgcacttca attgcaatca aattagttca ttctctttgc 6720 gcagttccct acctctcctt tcaaggttcg tagatttctt ccgttttttt ttcttcttct 6780 ttattgtttg ttctacatca gcatgatgtt gatttgattg tgttttctat cgtttcatcg 6840 attataaatt ttcataatca gaagattcag cttttattaa tgcaagaacg tccttaattg 6900 atgattttat aaccgtaaat taggtctaat tagagttttt ttcataaaga ttttcagatc 6960 cgtttacaac aagccttaat tgttgattct gtagtcgtag attaaggttt ttttcatgaa 7020 ctacttcaga tccgttaaac aacagcctta tttgttgata cttcagtcgt ttttcaagaa 7080 attgttcaga tccgttgata aaagccttat tcgttgattc tgtatggtat ttcaagagat 7140 attgctcagg tcctttagca actaccttat ttgttgattc tgtggccata gattaggatt 7200 ttttttcacg aaattgcttc ttgaaattac gtgatggatt ttgattctga tttatcttgt 7260 gattgttgac tctacagcca tggcccacag caagcacggc ctgaaggagg agatgaccat 7320 gaagtaccac atggagggct gcgtgaacgg ccacaagttc gtgatcaccg gcgagggcat 7380 cggctacccc ttcaagggca agcagaccat caacctgtgc gtgatcgagg gcggccccct 7440 gcccttcagc gaggacatcc tgagcgccgg cttcaagtac ggcgaccgga tcttcaccga 7500 gtacccccag gacatcgtgg actacttcaa gaacagctgc cccgccggct acacctgggg 7560 ccggagcttc ctgttcgagg acggcgccgt gtgcatctgt aacgtggaca tcaccgtgag 7620 cgtgaaggag aactgcatct accacaagag catcttcaac ggcgtgaact tccccgccga 7680 cggccccgtg atgaagaaga tgaccaccaa ctgggaggcc agctgcgaga agatcatgcc 7740 cgtgcctaag cagggcatcc tgaagggcga cgtgagcatg tacctgctgc tgaaggacgg 7800 cggccggtac cggtgccagt tcgacaccgt gtacaaggcc aagagcgtgc ccagcaagat 7860 gcccgagtgg cacttcatcc agcacaagct gctgcgggag gaccggagcg acgccaagaa 7920 ccagaagtgg cagctgaccg agcacgccat cgccttcccc agcgccctgg cctgagagct 7980 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg 8040 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta 8100 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat 8160 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg 8220 cggtgtcatc tatgttacta gatcgggaat tctagtggcc ggcccagctg atatctagac 8280 ccagctttct tgtacaaagt ggccgttaac ggatcggcca gaatggcccg gaccgggtta 8340 ccgaattcga gctcggtacc ctgggatccg atatcgatgg gccctggccg aagcttggtc 8400 acccggtccg ggcctagaag gccagcttcg gccgccccgg gcaactttat tatacaaagt 8460 tgatggtacg gatccgtcga cggcgcgccg cggacctgaa ttccagcaca ctggcggccg 8520 ttagtctaaa aacggcccct aactaacgct tagttgcccg ctagattagt cccccgttag 8580 tgcctagtta gacacacgac gaactagggg ctactagtaa cggccgccag tgtgctcggt 8640 acggccagaa tggccatctg gattcagcag ggggtgattg cggttacatc atgtacggaa 8700 aaataattct aatccttgat ttaaatttga acttgactat ttatttattc tttatttcat 8760 tttgtaaatc attttatgta tctcctggca agcaatttta tccaccttgc accaacacct 8820 tcgggttcca taatcaaacc accttaactt cacaccatgc tgtaactcac accgcccagc 8880 atctccaatg tgaaagaagc taaaatttaa taaacaatca tacgaagcag tgacaaaata 8940 ccagatggta ttaatgcttc gataaaatta attggaaagt ataaaatggt agaaaataat 9000 aaattataat taatttaagt aagataaaaa ataattaaaa actaaaatgt taaaatttta 9060 aaaaaattat tttaaataat atttaaaaac attaaaaatc attttaaaaa atttatttat 9120 agaacaatta aataaatatt tcagctaata aaaaacaaaa gcttacctag ccttagaaga 9180 caacttgtcc aacaattaga tgatacccat tgcccttacg ttttctttaa catcaattat 9240 tgtttttgtc aacaagctat cttttagttt tattttattg gtaaaaaata tgtcgccttc 9300 aagttgcatc atttaacaca tctcgtcatt agaaaaataa aactcttccc taaacgatta 9360 gtagaaaaaa tcattcgata ataaataaga aagaaaaatt agaaaaaaat aacttcattt 9420 taaaaaaatc attaaggcta tattttttaa atgactaatt ttatatagac tgtaactaaa 9480 agtatacaat ttattatgct atgtatctta aagaattact tataaaaatc tacggaagaa 9540 tatcttacaa agtgaaaaac aaatgagaaa gaatttagtg ggatgattat gattttattt 9600 gaaaattgaa aaaataatta ttaaagactt tagtggagta agaaagcttt cctattagtc 9660 ttttcttatc cataaaaaaa aaaaaaaaaa tctagcgtga cagcttttcc atagatttta 9720 ataatgtaaa atactggtag cagccgaccg ttcaggtaat ggacactgtg gtcctaactt 9780 gcaacgggtg cgggcccaat ttaataacgc cgtggtaacg gataaagcca agcgtgaagc 9840 ggtgaaggta catctctgac tccgtcaaga ttacgaaacc gtcaactacg aaggactccc 9900 cgaaatatca tctgtgtcat aaacaccaag tcacaccata catgggcacg cgtcacaata 9960 tgattggaga acggttccac cgcatatgct ataaaatgcc cccacacccc tcgaccctaa 10020 tcgcacttca attgcaatca aattagttca ttctctttgc gcagttccct acctctcctt 10080 tcaaggttcg tagatttctt ccgttttttt ttcttcttct ttattgtttg ttctacatca 10140 gcatgatgtt gatttgattg tgttttctat cgtttcatcg attataaatt ttcataatca 10200 gaagattcag cttttattaa tgcaagaacg tccttaattg atgattttat aaccgtaaat 10260 taggtctaat tagagttttt ttcataaaga ttttcagatc cgtttacaac aagccttaat 10320 tgttgattct gtagtcgtag attaaggttt ttttcatgaa ctacttcaga tccgttaaac 10380 aacagcctta tttgttgata cttcagtcgt ttttcaagaa attgttcaga tccgttgata 10440 aaagccttat tcgttgattc tgtatggtat ttcaagagat attgctcagg tcctttagca 10500 actaccttat ttgttgattc tgtggccata gattaggatt ttttttcacg aaattgcttc 10560 ttgaaattac gtgatggatt ttgattctga tttatcttgt gattgttgac tctacagcca 10620 tggctgcaac tactcttaca tctgctttac ctggtgcttt cagttcatcc cagagaccaa 10680 gtgctccttt caacttacaa agatcaccta gagtgcttag aaggttcaac agaaagacag 10740 gacgtcaacc tcgtggactt gttcgtgctg caaaggctca agcggcgcaa aacctgccag 10800 agaaattcga tgaaacaaga ctctgggagg ccctcagggg atttggggtt agtccaagta 10860 gggttactta cgctcccgtc ggattcggag attaccactg gaccgtcaca gatgaagacg 10920 ggagaccttg gttcgcaact gtcagtgacc tcgaacacaa agagcattgc gggcagggag 10980 cacaagcagc ccttaaagga ctcagacaag ctatggacac tgccctcgca cttagagata 11040 gggacggact cagattcgtg gttgctccag tggcagcaac agatgggggt ggccctgtcc 11100 ttccccttga cgctaggtat gcactcacag tcttccctca cgtcccagga agaacaggag 11160 agttcggaca aagactcaca gaggcagaga gggacagact gcttgctctt ctggcagaac 11220 tccacggaag aacacctcct gaaacaaccc caccagccga tatggaacca ccaggacttc 11280 ctggtgttag agcagcactc gccgaaagtg aaggaccttg gagtggagga ccatttgcag 11340 aacccgctag acttcttctc agagaacacg aggccacact gcacgcaaga ctcgctgaat 11400 tcgaagccct cgtcgcaaga gttaagggtc gtggagcacc tcttgtcgtt acccacggag 11460 aaccacaccc aggcaacttg atcctcggag aggaaggcta tctcctcgtg gactgggata 11520 cagttggact ggctccagct gaaagggatt tgagtctcat ctcagacgac ccagctgacc 11580 tcgcaagata cgcagagctt acaggaagaa ctccagaccc agacgcactc gcactctata 11640 gactgcgttg gagtctcctt gatgtggctg agttcgtcga gtggttcagg gctgaacacc 11700 aaagaaccca agacaccgag agtgcatgga aggggttcac tgacacactt gggcaattgg 11760 ctgctggtgg atcagctgca tagcaaactt atctctgtta tgaatcagaa gaagttcatg 11820 tctcgtttca tttaaaactt tggtggtttg tgttttgggg ccttgtaaag cccctgatga 11880 ataattgttc aactatgttt ccgttcctgt gttatacctt tctttctaat gagtaatgac 11940 atcaaacttc ttctgtattg aaattatgtc cttgtgagtc tctttatcat cgtttcgtct 12000 ttacattata tgtgctactt ttgtctaatg agcctgaaaa gtggctccaa tggtacgcac 12060 tggaagattt gttggcttct ggtagatata gcgacagtgt tgagcttgta atatcatgtc 12120 tcttattgct aaattagttc ctttcttaac agaaaccttc aaagtttttg tttttgtttt 12180 catttaccta atgtacacat acgctggcca tgactaacaa catgtccagg cttagagcat 12240 atttttttct agcttaaatt gttaacttgt cattcagtaa aatccgagaa ttgtgaagct 12300 ctaattgaag ctaattcgtt ttataaagtc agttaaaaag tatactaaat tatccaactt 12360 ttcttcaaaa tctcaaaatt ctatgacaaa acgatagtct ttgtttatgt cagtaccaca 12420 aagaggtgga aaaaaacacc aaaaaaacaa taagcaaact atacactgag aagaaaaata 12480 aaagagagct caatagatgt tttatactaa cggtagatta gatcaaagat ccaagcttta 12540 ctctacatag agcagaaccc agaatccctt catatctctt ttattctagc accgataatc 12600 tactgaaaag aagacactta gagctctgtc tctttgtcaa agaagtccca gccgtcatcc 12660 agaagctcct tacgttcatt aacagaggcc gcaattggat cgggtttact tattttgtgg 12720 gtatctatac ttttattaga tttttaatca ggctcctgat ttctttttat ttcgattgaa 12780 ttcctgaact tgtattattc agtagatcga ataaattata aaaagataaa atcataaaat 12840 aatattttat cctatcaatc atattaaagc aatgaatatg taaaattaat cttatcttta 12900 ttttaaaaaa tcatataggt ttagtatttt tttaaaaata aagataggat tagttttact 12960 attcactgct tattactttt aaaaaaatca taaaggttta gtattttttt aaaataaata 13020 taggaatagt tttactattc actgctttaa tagaaaaata gtttaaaatt taagatagtt 13080 ttaatcccag catttgccac gtttgaacgt gagccgaaac gatgtcgtta cattatctta 13140 acctagctga aacgatgtcg tcataatatc gccaaatgcc aactggacta cgtcgaaccc 13200 acaaatccca caaagcgcgt gaaatcaaat cgctcaaacc acaaaaaaga acaacgcgtt 13260 tgttacacgc tcaatcccac gcgagtagag cacagtaacc ttcaaataag cgaatggggc 13320 ataatcagaa atccgaaata aacctagggg cattatcgga aatgaaaagt agctcactca 13380 atataaaaat ctaggaaccc tagttttcgt tatcactctg tgctccctcg ctctatttct 13440 cagtctctgt gtttgcggct gaggattccg aacgagtgac cttcttcgtt tctcgcaaag 13500 gtaacagcct ctgctcttgt ctcttcgatt cgatctatgc ctgtctctta tttacgatga 13560 tgtttcttcg gttatgtttt tttatttatg ctttatgctg ttgatgttcg gttgtttgtt 13620 tcgctttgtt tttgtggttc agttttttag gattcttttg gtttttgaat cgattaatcg 13680 gaagagattt tcgagttatt tggtgtgttg gaggtgaatc ttttttttga ggtcatagat 13740 ctgttgtatt tgtgttataa acatgcgact ttgtatgatt ttttacgagg ttatgatgtt 13800 ctggttgttt tattatgaat ctgttgagac agaaccatga tttttgttga tgttcgttta 13860 cactattaaa ggtttgtttt aacaggatta aaagtttttt aagcatgttg aaggagtctt 13920 gtagatatgt aaccgtcgat agtttttttg tgggtttgtt cacatgttat caagcttaat 13980 cttttactat gtatgcgacc atatctggat ccagcaaagg cgatttttta attccttgtg 14040 aaacttttgt aatatgaagt tgaaattttg ttattggtaa actataaatg tgtgaagttg 14100 gagtatacct ttaccttctt atttggcttt gtgatagttt aatttatatg tattttgagt 14160 tctgacttgt atttctttga attgattcta gtttaagtaa tcatggcctc ctccgagaac 14220 gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag 14280 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg 14340 aaggtgacga agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag 14400 tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc 14460 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggcgacc 14520 gtgacccagg actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc 14580 gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc 14640 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agacccacaa ggccctgaag 14700 ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc 14760 gtgcagctgc ccggctacta ctacgtggac gccaagctgg acatcacctc ccacaacgag 14820 gactacacca tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag 14880 ttttgtgatc tgatgataag tggttggttc gtgtctcatg cacttgggag gtgatctatt 14940 tcacctggtg tagtttgtgt ttccgtcagt tggaaaaact tatccctatc gatttcgttt 15000 tcattttctg cttttctttt atgtaccttc gtttgggctt gtaacgggcc tttgtatttc 15060 aactctcaat aataatccaa gtgcatgtta aacaatttgt catctgtttc ggctttgata 15120 tactactggt gaagatgggc cgtactactg catcacaacg aaaaataata ataagatgaa 15180 aaacttgaag tggaaaaaaa aaaaaacttg aatgttcact actactcatt gaccataatg 15240 tttaacatac atagctcaat agtatttttg tgaatatggc aacacaaaca gtccaaaaca 15300 attgtctctt actataccaa accaagggcg ccgcttgttt gccactcttt gtgtgcaata 15360 gtgtgattac cacatctcca cattcaatat attccctgaa ttatctgacg attttgatgg 15420 ctcactgttt tcccaagtct tgaattgtct tctgtgcgcc agtcaaatgc atatgtgttg 15480 agtttatctt ttaaatatca agcttttgtt tttaactttt gtttgtaacc aaaaactcac 15540 agtaggagtt tgatcacata attttatgtt tgcctttgca atttctagtg agtctttgat 15600 taaaagcttg aaaagaaaat gcagccaagc ttaccaagta agttatgtgt attaaccaga 15660 ggaagagaga atcttgcaaa atttcaacaa acacaaaaag aagtattact acgattggtg 15720 gagaaagaaa acgattccaa atcttgaact gttgttgtaa aagcatagca gaaagtggga 15780 gacaaccgaa atagaaatga ctataactta atttaatgtt atcattataa tttcttctag 15840 caaatattta gaaagtaaat atcacatcaa cctttaatgt aattaagctt tctctttttg 15900 attcatgtga gatgaaaaga aaaaaaagaa gagaaaagtg tagaaaacac atcatttcta 15960 agctgaagct gaggtacaaa ttaattaata agtgactagg gtcacgtgac cctagtcact 16020 tatgattccg atgacttcgt aggttcctag ctcaagccgc tcgtgtccaa gcgtcactta 16080 cgattagcta atgattacgg catctaggac cgactagttc ttatgtgctt ctagtctcca 16140 aatgtggttg atagttattt tgctctaaga tcaacagtaa tgaagtataa atcatcgttg 16200 tggtgtgcta ctcggttaat tgagcattaa cacacacaaa catgacgagg atggtataat 16260 ctccaaaaat gtgtactttg ttaggtggga ccctatagcc ttgattaatg tgctatgtta 16320 ggcatgcctg gaaacgtgtg acgcatatgt tttgtgaacc tgttgatatt atatgtgctt 16380 ttatattacc atattttatt aaaatactaa tatttattac tagtaagata taacattcta 16440 tctagcttaa aaactaacca taaatattcc ataataacta gatttaccaa actaatatac 16500 taaatataca taataaatac aaaattaaca agacaataat caatatttat gagcttaata 16560 tatttagaca ttatggttgg tcgacgataa tcatgctaac ttttcgtaat tgcttgattg 16620 aaatatgctt agaataatgc ctctttgttc tacatggcaa atagggacca ttatggtgta 16680 acaccctggg aaccacaaac accccgaaat gctactaaac tacacaacta accttcatat 16740 ataaaatttc gacagcatct cctttgaaaa tttgcataga cgtggaagca acagagtata 16800 aacagatcta gctagctaag tttaaacggc cggccaaacg tgcaagcgct actagacaat 16860 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttatttac 16920 aattgaatat atcctgcca 16939 <210> 172 <211> 16238 <212> DNA <213> Artificial Sequence <220> <223> RV027338 T-DNA <400> 172 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgcatatc ggttgttgac 1440 gattaaccat gcaaaaaaga aacattaatt gcgaatgtaa ataacaaaac atgtaactct 1500 tgtagatata catgtatcga catttaaacc cgaatatata tgtataccta taatttctct 1560 gattttcacg ctacctgcca cgtacatggg tgataggtcc aaactcacaa gtaaaagttt 1620 acgtacagtg aattcgtctt tttgggtata aacgtacatt taatttacac gtaagaaagg 1680 attaccaatt ctttcattta tggtaccaga cagagttaag gcaaacaaga gaaacatata 1740 gagttttgat atgttttctt ggataaatat taaattgatg caatatttag ggatggacac 1800 aaggtaatat atgcctttta aggtatatgt gctatatgaa tcgtttcgca tgggtactaa 1860 aattatttgt ccttacttta tataaacaaa ttccaacaaa atcaagtttt tgctaaaact 1920 agtttatttg cgggttattt aattacctat catattactt gtaatatcat tcgtatgtta 1980 acgggtaaac caaaccaaac cggatattga actattaaaa atcttgtaaa tttgacacaa 2040 actaatgaat atctaaatta tgttactgct atgataacga ccatttttgt ttttgagaac 2100 cataatataa attacaggta cgtgacaagt actaagtatt tatatccacc tttagtcaca 2160 gtaccaatat tgcgcctacc gggcaacgtg aacgtgatca tcaaatcaaa gtagttacca 2220 aacgctttga tctcgataaa actaaaagct gacacgtctt gctgtttctt aatttatttc 2280 tcttacaacg acaattttga gaaatatgaa atttttatat cgaaagggaa cagtccttat 2340 catttgctcc catcacttgc ttttgtctag ttacaactgg aaatcgaaga gaagtattac 2400 aaaaacattt ttctcgtcat ttataaaaaa atgacaaaaa attaaataga gagcaaagca 2460 agagcgttgg gtgacgttgg tctcttcatt aactcctctc atctacccct tcctctgttc 2520 gcctttatat ccttcacctt ccctctctca tcttcattaa ctcatcttca aaaataccct 2580 aatcacattt tgtaacaata atacaattat acattaaaac tctccgacgc catggaaact 2640 caacaacaac aagcagatat tcaagatttt gggaacaaaa acagtaacac ctatgcatgc 2700 aggcaaagca gtactaggtg gacacccaca agtgatcaga tcagaatact gaaagagctc 2760 tactacaaca atggaatcag atcaccaact gctgatcaga ttcagagaat cgctgctcaa 2820 ctcagacagt acggcaagat tgaaggcaag aacgtgtttt attggtttca gaaccataaa 2880 gctcgtgaac gccagaagaa acggttcact cctactgctc ctccaccacc gtctcacttc 2940 tccgatcata ttaaccacca tcttcccaac gctgccccca tgcagattcc gtctcaccac 3000 caccactacc accatcaaga accaccacat gtctatgctc accctcacaa actctacacc 3060 actcatcaca ttggggttgg ttcttcttca caaggagtga tgggtgtagg gtgtggctat 3120 ggatctgttg ctatggagaa gagtttcagg aagtgttcaa tatcaccacc gggagagagt 3180 aaggccacag gaggaattgg ccggaatatc ggttcaagat cacggataag tgtcgattcg 3240 tgttccttct tcgacacaat taagccaaag acatatgaga tgtttgagaa tcatgatcaa 3300 gatgaagaac aaggagagcc ttcaactgag atcgaaacac ttccattatt cccgattcac 3360 gacggcaacc atcatgattt cttcggcatg aggacggcaa atctgcaatt ggagcaaggc 3420 actggaggtt actacactgg aggaagctgg tatcggtctg atgagagggc gtctcttgag 3480 ctcagtctca actcatatgg ttattataac taattcgaac tggccaggta cctgacatct 3540 tatagtctgc aacctctcgt gtctgaattc ctatctttat caagtgttat tgcttccacg 3600 actataggac agctttcgtc gaaaggtttt gctcatgtga tctcgaagga ttcatctagt 3660 ctgatttttc gtgacttgta tcggttttat tggattcatc caacatatat caataaaaaa 3720 tgagttgtgt ttcctttctt cctagttcag ttaaaattat ttccctcctg cgcttgtgct 3780 gtaattgtct gtgtacctgt tgtttgtgac tgtgttagtt cccttggata tgatttcgta 3840 tttgatatgt acatggagat agcttagctt cattattgga gtatgaagtt agtatgacat 3900 agtcactctc ctggaaaatt gacactgcaa accatatttt tattctgaac cacaaatcct 3960 agtcagtccg ctggcatatg ccgtccgttt gctgaatcca gaacgtgggt ttggagatgt 4020 acggctgaga tgcctctatg cgaaggggat ttcgtggtga aacgagatgg gagtagagca 4080 acgcccgtgg aagatgcttc aaacttccac acttttgagc aacgatcggc agtagtaagg 4140 tagacgattt caagatcaaa gcatatgaag ataaacaaca tcaacaacaa aatttgttgg 4200 ggttctatag agagaaacag agctacatac atacactgtt ttgtatctac catctgagat 4260 gatgaaaaga tgaaaaacta aagaatgccc cggcgccaac gccaggacac gccgcgcgcg 4320 cgtcacccga gccatctctt gacccagccg gcgctgtata tttacacacg ttgcagcatc 4380 gatcaccacc tgttcgatcg cgtcgccgtc accaagcttg gcgcgccggt cacccggtcc 4440 gggcctagaa ggccacccgg ggctcggcgt taggctttta gtccgctagg tggctagacc 4500 gaggttagga cgctagttca ctaggggcct agtggtgttt taaggatggg caagtttgta 4560 caaaaaagca ggctggcgcc ggaaccaatt cagtcgagtg cggccgccag tgtgatggat 4620 atctcgaccc gggtgattgc ggttacatca tgtacggaaa aataattcta atccttgatt 4680 taaatttgaa cttgactatt tatttattct ttatttcatt ttgtaaatca ttttatgtat 4740 ctcctggcaa gcaattttat ccaccttgca ccaacacctt cgggttccat aatcaaacca 4800 ccttaacttc acaccatgct gtaactcaca ccgcccagca tctccaatgt gaaagaagct 4860 aaaatttaat aaacaatcat acgaagcagt gacaaaatac cagatggtat taatgcttcg 4920 ataaaattaa ttggaaagta taaaatggta gaaaataata aattataatt aatttaagta 4980 agataaaaaa taattaaaaa ctaaaatgtt aaaattttaa aaaaattatt ttaaataata 5040 tttaaaaaca ttaaaaatca ttttaaaaaa tttatttata gaacaattaa ataaatattt 5100 cagctaataa aaaacaaaag cttacctagc cttagaagac aacttgtcca acaattagat 5160 gatacccatt gcccttacgt tttctttaac atcaattatt gtttttgtca acaagctatc 5220 ttttagtttt attttattgg taaaaaatat gtcgccttca agttgcatca tttaacacat 5280 ctcgtcatta gaaaaataaa actcttccct aaacgattag tagaaaaaat cattcgataa 5340 taaataagaa agaaaaatta gaaaaaaata acttcatttt aaaaaaatca ttaaggctat 5400 attttttaaa tgactaattt tatatagact gtaactaaaa gtatacaatt tattatgcta 5460 tgtatcttaa agaattactt ataaaaatct acggaagaat atcttacaaa gtgaaaaaca 5520 aatgagaaag aatttagtgg gatgattatg attttatttg aaaattgaaa aaataattat 5580 taaagacttt agtggagtaa gaaagctttc ctattagtct tttcttatcc ataaaaaaaa 5640 aaaaaaaaat ctagcgtgac agcttttcca tagattttaa taatgtaaaa tactggtagc 5700 agccgaccgt tcaggtaatg gacactgtgg tcctaacttg caacgggtgc gggcccaatt 5760 taataacgcc gtggtaacgg ataaagccaa gcgtgaagcg gtgaaggtac atctctgact 5820 ccgtcaagat tacgaaaccg tcaactacga aggactcccc gaaatatcat ctgtgtcata 5880 aacaccaagt cacaccatac atgggcacgc gtcacaatat gattggagaa cggttccacc 5940 gcatatgcta taaaatgccc ccacacccct cgaccctaat cgcacttcaa ttgcaatcaa 6000 attagttcat tctctttgcg cagttcccta cctctccttt caaggttcgt agatttcttc 6060 cgtttttttt tcttcttctt tattgtttgt tctacatcag catgatgttg atttgattgt 6120 gttttctatc gtttcatcga ttataaattt tcataatcag aagattcagc ttttattaat 6180 gcaagaacgt ccttaattga tgattttata accgtaaatt aggtctaatt agagtttttt 6240 tcataaagat tttcagatcc gtttacaaca agccttaatt gttgattctg tagtcgtaga 6300 ttaaggtttt tttcatgaac tacttcagat ccgttaaaca acagccttat ttgttgatac 6360 ttcagtcgtt tttcaagaaa ttgttcagat ccgttgataa aagccttatt cgttgattct 6420 gtatggtatt tcaagagata ttgctcaggt cctttagcaa ctaccttatt tgttgattct 6480 gtggccatag attaggattt tttttcacga aattgcttct tgaaattacg tgatggattt 6540 tgattctgat ttatcttgtg attgttgact ctacagccat ggcccacagc aagcacggcc 6600 tgaaggagga gatgaccatg aagtaccaca tggagggctg cgtgaacggc cacaagttcg 6660 tgatcaccgg cgagggcatc ggctacccct tcaagggcaa gcagaccatc aacctgtgcg 6720 tgatcgaggg cggccccctg cccttcagcg aggacatcct gagcgccggc ttcaagtacg 6780 gcgaccggat cttcaccgag tacccccagg acatcgtgga ctacttcaag aacagctgcc 6840 ccgccggcta cacctggggc cggagcttcc tgttcgagga cggcgccgtg tgcatctgta 6900 acgtggacat caccgtgagc gtgaaggaga actgcatcta ccacaagagc atcttcaacg 6960 gcgtgaactt ccccgccgac ggccccgtga tgaagaagat gaccaccaac tgggaggcca 7020 gctgcgagaa gatcatgccc gtgcctaagc agggcatcct gaagggcgac gtgagcatgt 7080 acctgctgct gaaggacggc ggccggtacc ggtgccagtt cgacaccgtg tacaaggcca 7140 agagcgtgcc cagcaagatg cccgagtggc acttcatcca gcacaagctg ctgcgggagg 7200 accggagcga cgccaagaac cagaagtggc agctgaccga gcacgccatc gccttcccca 7260 gcgccctggc ctgagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc 7320 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 7380 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 7440 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 7500 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ctagtggccg 7560 gcccagctga tatctagacc cagctttctt gtacaaagtg gccgttaacg gatcggccag 7620 aatggcccgg accgggttac cgaattcgag ctcggtaccc tgggatccga tatcgatggg 7680 ccctggccga agcttggtca cccggtccgg gcctagaagg ccagcttcgg ccgccccggg 7740 caactttatt atacaaagtt gatggtacgg atccgtcgac ggcgcgccgc ggacctgaat 7800 tccagcacac tggcggccgt tagtctaaaa acggccccta actaacgctt agttgcccgc 7860 tagattagtc ccccgttagt gcctagttag acacacgacg aactaggggc tactagtaac 7920 ggccgccagt gtgctcggta cggccagaat ggccatctgg attcagcagg gggtgattgc 7980 ggttacatca tgtacggaaa aataattcta atccttgatt taaatttgaa cttgactatt 8040 tatttattct ttatttcatt ttgtaaatca ttttatgtat ctcctggcaa gcaattttat 8100 ccaccttgca ccaacacctt cgggttccat aatcaaacca ccttaacttc acaccatgct 8160 gtaactcaca ccgcccagca tctccaatgt gaaagaagct aaaatttaat aaacaatcat 8220 acgaagcagt gacaaaatac cagatggtat taatgcttcg ataaaattaa ttggaaagta 8280 taaaatggta gaaaataata aattataatt aatttaagta agataaaaaa taattaaaaa 8340 ctaaaatgtt aaaattttaa aaaaattatt ttaaataata tttaaaaaca ttaaaaatca 8400 ttttaaaaaa tttatttata gaacaattaa ataaatattt cagctaataa aaaacaaaag 8460 cttacctagc cttagaagac aacttgtcca acaattagat gatacccatt gcccttacgt 8520 tttctttaac atcaattatt gtttttgtca acaagctatc ttttagtttt attttattgg 8580 taaaaaatat gtcgccttca agttgcatca tttaacacat ctcgtcatta gaaaaataaa 8640 actcttccct aaacgattag tagaaaaaat cattcgataa taaataagaa agaaaaatta 8700 gaaaaaaata acttcatttt aaaaaaatca ttaaggctat attttttaaa tgactaattt 8760 tatatagact gtaactaaaa gtatacaatt tattatgcta tgtatcttaa agaattactt 8820 ataaaaatct acggaagaat atcttacaaa gtgaaaaaca aatgagaaag aatttagtgg 8880 gatgattatg attttatttg aaaattgaaa aaataattat taaagacttt agtggagtaa 8940 gaaagctttc ctattagtct tttcttatcc ataaaaaaaa aaaaaaaaat ctagcgtgac 9000 agcttttcca tagattttaa taatgtaaaa tactggtagc agccgaccgt tcaggtaatg 9060 gacactgtgg tcctaacttg caacgggtgc gggcccaatt taataacgcc gtggtaacgg 9120 ataaagccaa gcgtgaagcg gtgaaggtac atctctgact ccgtcaagat tacgaaaccg 9180 tcaactacga aggactcccc gaaatatcat ctgtgtcata aacaccaagt cacaccatac 9240 atgggcacgc gtcacaatat gattggagaa cggttccacc gcatatgcta taaaatgccc 9300 ccacacccct cgaccctaat cgcacttcaa ttgcaatcaa attagttcat tctctttgcg 9360 cagttcccta cctctccttt caaggttcgt agatttcttc cgtttttttt tcttcttctt 9420 tattgtttgt tctacatcag catgatgttg atttgattgt gttttctatc gtttcatcga 9480 ttataaattt tcataatcag aagattcagc ttttattaat gcaagaacgt ccttaattga 9540 tgattttata accgtaaatt aggtctaatt agagtttttt tcataaagat tttcagatcc 9600 gtttacaaca agccttaatt gttgattctg tagtcgtaga ttaaggtttt tttcatgaac 9660 tacttcagat ccgttaaaca acagccttat ttgttgatac ttcagtcgtt tttcaagaaa 9720 ttgttcagat ccgttgataa aagccttatt cgttgattct gtatggtatt tcaagagata 9780 ttgctcaggt cctttagcaa ctaccttatt tgttgattct gtggccatag attaggattt 9840 tttttcacga aattgcttct tgaaattacg tgatggattt tgattctgat ttatcttgtg 9900 attgttgact ctacagccat ggctgcaact actcttacat ctgctttacc tggtgctttc 9960 agttcatccc agagaccaag tgctcctttc aacttacaaa gatcacctag agtgcttaga 10020 aggttcaaca gaaagacagg acgtcaacct cgtggacttg ttcgtgctgc aaaggctcaa 10080 gcggcgcaaa acctgccaga gaaattcgat gaaacaagac tctgggaggc cctcagggga 10140 tttggggtta gtccaagtag ggttacttac gctcccgtcg gattcggaga ttaccactgg 10200 accgtcacag atgaagacgg gagaccttgg ttcgcaactg tcagtgacct cgaacacaaa 10260 gagcattgcg ggcagggagc acaagcagcc cttaaaggac tcagacaagc tatggacact 10320 gccctcgcac ttagagatag ggacggactc agattcgtgg ttgctccagt ggcagcaaca 10380 gatgggggtg gccctgtcct tccccttgac gctaggtatg cactcacagt cttccctcac 10440 gtcccaggaa gaacaggaga gttcggacaa agactcacag aggcagagag ggacagactg 10500 cttgctcttc tggcagaact ccacggaaga acacctcctg aaacaacccc accagccgat 10560 atggaaccac caggacttcc tggtgttaga gcagcactcg ccgaaagtga aggaccttgg 10620 agtggaggac catttgcaga acccgctaga cttcttctca gagaacacga ggccacactg 10680 cacgcaagac tcgctgaatt cgaagccctc gtcgcaagag ttaagggtcg tggagcacct 10740 cttgtcgtta cccacggaga accacaccca ggcaacttga tcctcggaga ggaaggctat 10800 ctcctcgtgg actgggatac agttggactg gctccagctg aaagggattt gagtctcatc 10860 tcagacgacc cagctgacct cgcaagatac gcagagctta caggaagaac tccagaccca 10920 gacgcactcg cactctatag actgcgttgg agtctccttg atgtggctga gttcgtcgag 10980 tggttcaggg ctgaacacca aagaacccaa gacaccgaga gtgcatggaa ggggttcact 11040 gacacacttg ggcaattggc tgctggtgga tcagctgcat agcaaactta tctctgttat 11100 gaatcagaag aagttcatgt ctcgtttcat ttaaaacttt ggtggtttgt gttttggggc 11160 cttgtaaagc ccctgatgaa taattgttca actatgtttc cgttcctgtg ttataccttt 11220 ctttctaatg agtaatgaca tcaaacttct tctgtattga aattatgtcc ttgtgagtct 11280 ctttatcatc gtttcgtctt tacattatat gtgctacttt tgtctaatga gcctgaaaag 11340 tggctccaat ggtacgcact ggaagatttg ttggcttctg gtagatatag cgacagtgtt 11400 gagcttgtaa tatcatgtct cttattgcta aattagttcc tttcttaaca gaaaccttca 11460 aagtttttgt ttttgttttc atttacctaa tgtacacata cgctggccat gactaacaac 11520 atgtccaggc ttagagcata tttttttcta gcttaaattg ttaacttgtc attcagtaaa 11580 atccgagaat tgtgaagctc taattgaagc taattcgttt tataaagtca gttaaaaagt 11640 atactaaatt atccaacttt tcttcaaaat ctcaaaattc tatgacaaaa cgatagtctt 11700 tgtttatgtc agtaccacaa agaggtggaa aaaaacacca aaaaaacaat aagcaaacta 11760 tacactgaga agaaaaataa aagagagctc aatagatgtt ttatactaac ggtagattag 11820 atcaaagatc caagctttac tctacataga gcagaaccca gaatcccttc atatctcttt 11880 tattctagca ccgataatct actgaaaaga agacacttag agctctgtct ctttgtcaaa 11940 gaagtcccag ccgtcatcca gaagctcctt acgttcatta acagaggccg caattggatc 12000 gggtttactt attttgtggg tatctatact tttattagat ttttaatcag gctcctgatt 12060 tctttttatt tcgattgaat tcctgaactt gtattattca gtagatcgaa taaattataa 12120 aaagataaaa tcataaaata atattttatc ctatcaatca tattaaagca atgaatatgt 12180 aaaattaatc ttatctttat tttaaaaaat catataggtt tagtattttt ttaaaaataa 12240 agataggatt agttttacta ttcactgctt attactttta aaaaaatcat aaaggtttag 12300 tattttttta aaataaatat aggaatagtt ttactattca ctgctttaat agaaaaatag 12360 tttaaaattt aagatagttt taatcccagc atttgccacg tttgaacgtg agccgaaacg 12420 atgtcgttac attatcttaa cctagctgaa acgatgtcgt cataatatcg ccaaatgcca 12480 actggactac gtcgaaccca caaatcccac aaagcgcgtg aaatcaaatc gctcaaacca 12540 caaaaaagaa caacgcgttt gttacacgct caatcccacg cgagtagagc acagtaacct 12600 tcaaataagc gaatggggca taatcagaaa tccgaaataa acctaggggc attatcggaa 12660 atgaaaagta gctcactcaa tataaaaatc taggaaccct agttttcgtt atcactctgt 12720 gctccctcgc tctatttctc agtctctgtg tttgcggctg aggattccga acgagtgacc 12780 ttcttcgttt ctcgcaaagg taacagcctc tgctcttgtc tcttcgattc gatctatgcc 12840 tgtctcttat ttacgatgat gtttcttcgg ttatgttttt ttatttatgc tttatgctgt 12900 tgatgttcgg ttgtttgttt cgctttgttt ttgtggttca gttttttagg attcttttgg 12960 tttttgaatc gattaatcgg aagagatttt cgagttattt ggtgtgttgg aggtgaatct 13020 tttttttgag gtcatagatc tgttgtattt gtgttataaa catgcgactt tgtatgattt 13080 tttacgaggt tatgatgttc tggttgtttt attatgaatc tgttgagaca gaaccatgat 13140 ttttgttgat gttcgtttac actattaaag gtttgtttta acaggattaa aagtttttta 13200 agcatgttga aggagtcttg tagatatgta accgtcgata gtttttttgt gggtttgttc 13260 acatgttatc aagcttaatc ttttactatg tatgcgacca tatctggatc cagcaaaggc 13320 gattttttaa ttccttgtga aacttttgta atatgaagtt gaaattttgt tattggtaaa 13380 ctataaatgt gtgaagttgg agtatacctt taccttctta tttggctttg tgatagttta 13440 atttatatgt attttgagtt ctgacttgta tttctttgaa ttgattctag tttaagtaat 13500 catggcctcc tccgagaacg tcatcaccga gttcatgcgc ttcaaggtgc gcatggaggg 13560 caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 13620 ccacaacacc gtgaagctga aggtgacgaa gggcggcccc ctgcccttcg cctgggacat 13680 cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc 13740 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 13800 ggacggcggc gtggcgaccg tgacccagga ctcctccctg caggacggct gcttcatcta 13860 caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtgatgc agaagaagac 13920 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga 13980 gacccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat 14040 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggacg ccaagctgga 14100 catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgca ccgagggccg 14160 ccaccacctg ttcctgtagt tttgtgatct gatgataagt ggttggttcg tgtctcatgc 14220 acttgggagg tgatctattt cacctggtgt agtttgtgtt tccgtcagtt ggaaaaactt 14280 atccctatcg atttcgtttt cattttctgc ttttctttta tgtaccttcg tttgggcttg 14340 taacgggcct ttgtatttca actctcaata ataatccaag tgcatgttaa acaatttgtc 14400 atctgtttcg gctttgatat actactggtg aagatgggcc gtactactgc atcacaacga 14460 aaaataataa taagatgaaa aacttgaagt ggaaaaaaaa aaaaacttga atgttcacta 14520 ctactcattg accataatgt ttaacataca tagctcaata gtatttttgt gaatatggca 14580 acacaaacag tccaaaacaa ttgtctctta ctataccaaa ccaagggcgc cgcttgtttg 14640 ccactctttg tgtgcaatag tgtgattacc acatctccac attcaatata ttccctgaat 14700 tatctgacga ttttgatggc tcactgtttt cccaagtctt gaattgtctt ctgtgcgcca 14760 gtcaaatgca tatgtgttga gtttatcttt taaatatcaa gcttttgttt ttaacttttg 14820 tttgtaacca aaaactcaca gtaggagttt gatcacataa ttttatgttt gcctttgcaa 14880 tttctagtga gtctttgatt aaaagcttga aaagaaaatg cagccaagct taccaagtaa 14940 gttatgtgta ttaaccagag gaagagagaa tcttgcaaaa tttcaacaaa cacaaaaaga 15000 agtattacta cgattggtgg agaaagaaaa cgattccaaa tcttgaactg ttgttgtaaa 15060 agcatagcag aaagtgggag acaaccgaaa tagaaatgac tataacttaa tttaatgtta 15120 tcattataat ttcttctagc aaatatttag aaagtaaata tcacatcaac ctttaatgta 15180 attaagcttt ctctttttga ttcatgtgag atgaaaagaa aaaaaagaag agaaaagtgt 15240 agaaaacaca tcatttctaa gctgaagctg aggtacaaat taattaataa gtgactaggg 15300 tcacgtgacc ctagtcactt atgattccga tgacttcgta ggttcctagc tcaagccgct 15360 cgtgtccaag cgtcacttac gattagctaa tgattacggc atctaggacc gactagttct 15420 tatgtgcttc tagtctccaa atgtggttga tagttatttt gctctaagat caacagtaat 15480 gaagtataaa tcatcgttgt ggtgtgctac tcggttaatt gagcattaac acacacaaac 15540 atgacgagga tggtataatc tccaaaaatg tgtactttgt taggtgggac cctatagcct 15600 tgattaatgt gctatgttag gcatgcctgg aaacgtgtga cgcatatgtt ttgtgaacct 15660 gttgatatta tatgtgcttt tatattacca tattttatta aaatactaat atttattact 15720 agtaagatat aacattctat ctagcttaaa aactaaccat aaatattcca taataactag 15780 atttaccaaa ctaatatact aaatatacat aataaataca aaattaacaa gacaataatc 15840 aatatttatg agcttaatat atttagacat tatggttggt cgacgataat catgctaact 15900 tttcgtaatt gcttgattga aatatgctta gaataatgcc tctttgttct acatggcaaa 15960 tagggaccat tatggtgtaa caccctggga accacaaaca ccccgaaatg ctactaaact 16020 acacaactaa ccttcatata taaaatttcg acagcatctc ctttgaaaat ttgcatagac 16080 gtggaagcaa cagagtataa acagatctag ctagctaagt ttaaacggcc ggccaaacgt 16140 gcaagcgcta ctagacaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc 16200 taagcgtcaa tttatttaca attgaatata tcctgcca 16238 <210> 173 <211> 16534 <212> DNA <213> Artificial Sequence <220> <223> RV027340 T-DNA <400> 173 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgctccat taattcatgt 1440 acacaaattt atttgtttaa agtatcattg gatccattac acttcagttt tcttagcatc 1500 tttataaaac tctcactttc atgatgtttt ttttttataa tttaatatat cctgtatgtt 1560 gaattttcag ttagtcatct atattttgtc ttaaattttg ccttctttag cattaattaa 1620 ttgatttctt gcctctaata tctttcattc ctctcctaat ttgctttctg actttctgat 1680 ttattgtatt cttctttaaa taatcttatc aatttctgca tagtcagaga tgaaaccttc 1740 aagaaaacaa taaagaggaa agattaaagg ttagtattcc cttgcctacc aaaaaaaaca 1800 gaaaggtttg tattctcttc gtatatcctt attttcatta tacgtttggt tttatatata 1860 aacacgtgta tatactttat agtagtagta tacatttaca tattaatgtg tgtaataaaa 1920 acgtttaaat acgatgatga tgataagaaa tagctggaaa aaattaatac atcacccagg 1980 ccttatgtca cgttggttgt aagttgtata ataactatat gttggtccgt cgcacaggtg 2040 cacagatata atactatttt tttcatttca attttttttt ctttcttaat cgccaaatag 2100 tagtttacac tttttcctga agagttttta ttttttgaaa atctactaat gattatattt 2160 aattaaattt tgttttgttc tcacgaaaat attgtttttt taataaacag gatagagttg 2220 ggaatttatt tgtacctact ttttcccatt ttatttgctt tgaacaaatg agtccaacat 2280 ttctcttttc gtcttctctt tcctatccca tctaaatcaa aactgatcaa aatacctcga 2340 aattttaaat taaatcacca tccatcaaag ctattatagt gtagaaattt taaagttttt 2400 tctttggaca acaattgatt tccatctaca ctacatatga tatgactctt gaatcaatca 2460 tagtttttat ttcagtctgc tgattagttt ttttcttttc ttttaaaagg gtaaaaaatc 2520 ggtagtttcg aataattttg agtatgtgaa aattcattat ttttgggttg gttattcaat 2580 aacaaattaa tttgtgattg tgatgtcttt tttatgcaag gctctttttt cttgctttca 2640 attagtgggt cctttgtttc ctacaatcat ctccaattct tctgtttgct tatatcattt 2700 tgtatgatct tcaagaagaa agataaacga ctccgaagaa agatttaagt tattgttgtt 2760 catacaaaaa gaaggttgta aagttgttga cgtggacaaa agtattgcca tatttttcca 2820 gcctcgaaga aatttccctt cttcttcaaa ccctaaattt tgacaaaact ctctactttt 2880 ttttgcttct agggtttgct ttcagtttcc ggagtataag aaaagccatg gaaactcaac 2940 aacaacaagc agatattcaa gattttggga acaaaaacag taacacctat gcatgcaggc 3000 aaagcagtac taggtggaca cccacaagtg atcagatcag aatactgaaa gagctctact 3060 acaacaatgg aatcagatca ccaactgctg atcagattca gagaatcgct gctcaactca 3120 gacagtacgg caagattgaa ggcaagaacg tgttttattg gtttcagaac cataaagctc 3180 gtgaacgcca gaagaaacgg ttcactccta ctgctcctcc accaccgtct cacttctccg 3240 atcatattaa ccaccatctt cccaacgctg cccccatgca gattccgtct caccaccacc 3300 actaccacca tcaagaacca ccacatgtct atgctcaccc tcacaaactc tacaccactc 3360 atcacattgg ggttggttct tcttcacaag gagtgatggg tgtagggtgt ggctatggat 3420 ctgttgctat ggagaagagt ttcaggaagt gttcaatatc accaccggga gagagtaagg 3480 ccacaggagg aattggccgg aatatcggtt caagatcacg gataagtgtc gattcgtgtt 3540 ccttcttcga cacaattaag ccaaagacat atgagatgtt tgagaatcat gatcaagatg 3600 aagaacaagg agagccttca actgagatcg aaacacttcc attattcccg attcacgacg 3660 gcaaccatca tgatttcttc ggcatgagga cggcaaatct gcaattggag caaggcactg 3720 gaggttacta cactggagga agctggtatc ggtctgatga gagggcgtct cttgagctca 3780 gtctcaactc atatggttat tataactaat tcgaactggc caggtacctg acatcttata 3840 gtctgcaacc tctcgtgtct gaattcctat ctttatcaag tgttattgct tccacgacta 3900 taggacagct ttcgtcgaaa ggttttgctc atgtgatctc gaaggattca tctagtctga 3960 tttttcgtga cttgtatcgg ttttattgga ttcatccaac atatatcaat aaaaaatgag 4020 ttgtgtttcc tttcttccta gttcagttaa aattatttcc ctcctgcgct tgtgctgtaa 4080 ttgtctgtgt acctgttgtt tgtgactgtg ttagttccct tggatatgat ttcgtatttg 4140 atatgtacat ggagatagct tagcttcatt attggagtat gaagttagta tgacatagtc 4200 actctcctgg aaaattgaca ctgcaaacca tatttttatt ctgaaccaca aatcctagtc 4260 agtccgctgg catatgccgt ccgtttgctg aatccagaac gtgggtttgg agatgtacgg 4320 ctgagatgcc tctatgcgaa ggggatttcg tggtgaaacg agatgggagt agagcaacgc 4380 ccgtggaaga tgcttcaaac ttccacactt ttgagcaacg atcggcagta gtaaggtaga 4440 cgatttcaag atcaaagcat atgaagataa acaacatcaa caacaaaatt tgttggggtt 4500 ctatagagag aaacagagct acatacatac actgttttgt atctaccatc tgagatgatg 4560 aaaagatgaa aaactaaaga atgccccggc gccaacgcca ggacacgccg cgcgcgcgtc 4620 acccgagcca tctcttgacc cagccggcgc tgtatattta cacacgttgc agcatcgatc 4680 accacctgtt cgatcgcgtc gccgtcacca agcttggcgc gccggtcacc cggtccgggc 4740 ctagaaggcc acccggggct cggcgttagg cttttagtcc gctaggtggc tagaccgagg 4800 ttaggacgct agttcactag gggcctagtg gtgttttaag gatgggcaag tttgtacaaa 4860 aaagcaggct ggcgccggaa ccaattcagt cgagtgcggc cgccagtgtg atggatatct 4920 cgacccgggt gattgcggtt acatcatgta cggaaaaata attctaatcc ttgatttaaa 4980 tttgaacttg actatttatt tattctttat ttcattttgt aaatcatttt atgtatctcc 5040 tggcaagcaa ttttatccac cttgcaccaa caccttcggg ttccataatc aaaccacctt 5100 aacttcacac catgctgtaa ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa 5160 tttaataaac aatcatacga agcagtgaca aaataccaga tggtattaat gcttcgataa 5220 aattaattgg aaagtataaa atggtagaaa ataataaatt ataattaatt taagtaagat 5280 aaaaaataat taaaaactaa aatgttaaaa ttttaaaaaa attattttaa ataatattta 5340 aaaacattaa aaatcatttt aaaaaattta tttatagaac aattaaataa atatttcagc 5400 taataaaaaa caaaagctta cctagcctta gaagacaact tgtccaacaa ttagatgata 5460 cccattgccc ttacgttttc tttaacatca attattgttt ttgtcaacaa gctatctttt 5520 agttttattt tattggtaaa aaatatgtcg ccttcaagtt gcatcattta acacatctcg 5580 tcattagaaa aataaaactc ttccctaaac gattagtaga aaaaatcatt cgataataaa 5640 taagaaagaa aaattagaaa aaaataactt cattttaaaa aaatcattaa ggctatattt 5700 tttaaatgac taattttata tagactgtaa ctaaaagtat acaatttatt atgctatgta 5760 tcttaaagaa ttacttataa aaatctacgg aagaatatct tacaaagtga aaaacaaatg 5820 agaaagaatt tagtgggatg attatgattt tatttgaaaa ttgaaaaaat aattattaaa 5880 gactttagtg gagtaagaaa gctttcctat tagtcttttc ttatccataa aaaaaaaaaa 5940 aaaaatctag cgtgacagct tttccataga ttttaataat gtaaaatact ggtagcagcc 6000 gaccgttcag gtaatggaca ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat 6060 aacgccgtgg taacggataa agccaagcgt gaagcggtga aggtacatct ctgactccgt 6120 caagattacg aaaccgtcaa ctacgaagga ctccccgaaa tatcatctgt gtcataaaca 6180 ccaagtcaca ccatacatgg gcacgcgtca caatatgatt ggagaacggt tccaccgcat 6240 atgctataaa atgcccccac acccctcgac cctaatcgca cttcaattgc aatcaaatta 6300 gttcattctc tttgcgcagt tccctacctc tcctttcaag gttcgtagat ttcttccgtt 6360 tttttttctt cttctttatt gtttgttcta catcagcatg atgttgattt gattgtgttt 6420 tctatcgttt catcgattat aaattttcat aatcagaaga ttcagctttt attaatgcaa 6480 gaacgtcctt aattgatgat tttataaccg taaattaggt ctaattagag tttttttcat 6540 aaagattttc agatccgttt acaacaagcc ttaattgttg attctgtagt cgtagattaa 6600 ggtttttttc atgaactact tcagatccgt taaacaacag ccttatttgt tgatacttca 6660 gtcgtttttc aagaaattgt tcagatccgt tgataaaagc cttattcgtt gattctgtat 6720 ggtatttcaa gagatattgc tcaggtcctt tagcaactac cttatttgtt gattctgtgg 6780 ccatagatta ggattttttt tcacgaaatt gcttcttgaa attacgtgat ggattttgat 6840 tctgatttat cttgtgattg ttgactctac agccatggcc cacagcaagc acggcctgaa 6900 ggaggagatg accatgaagt accacatgga gggctgcgtg aacggccaca agttcgtgat 6960 caccggcgag ggcatcggct accccttcaa gggcaagcag accatcaacc tgtgcgtgat 7020 cgagggcggc cccctgccct tcagcgagga catcctgagc gccggcttca agtacggcga 7080 ccggatcttc accgagtacc cccaggacat cgtggactac ttcaagaaca gctgccccgc 7140 cggctacacc tggggccgga gcttcctgtt cgaggacggc gccgtgtgca tctgtaacgt 7200 ggacatcacc gtgagcgtga aggagaactg catctaccac aagagcatct tcaacggcgt 7260 gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc accaactggg aggccagctg 7320 cgagaagatc atgcccgtgc ctaagcaggg catcctgaag ggcgacgtga gcatgtacct 7380 gctgctgaag gacggcggcc ggtaccggtg ccagttcgac accgtgtaca aggccaagag 7440 cgtgcccagc aagatgcccg agtggcactt catccagcac aagctgctgc gggaggaccg 7500 gagcgacgcc aagaaccaga agtggcagct gaccgagcac gccatcgcct tccccagcgc 7560 cctggcctga gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa 7620 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta 7680 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta 7740 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg 7800 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattctag tggccggccc 7860 agctgatatc tagacccagc tttcttgtac aaagtggccg ttaacggatc ggccagaatg 7920 gcccggaccg ggttaccgaa ttcgagctcg gtaccctggg atccgatatc gatgggccct 7980 ggccgaagct tggtcacccg gtccgggcct agaaggccag cttcggccgc cccgggcaac 8040 tttattatac aaagttgatg gtacggatcc gtcgacggcg cgccgcggac ctgaattcca 8100 gcacactggc ggccgttagt ctaaaaacgg cccctaacta acgcttagtt gcccgctaga 8160 ttagtccccc gttagtgcct agttagacac acgacgaact aggggctact agtaacggcc 8220 gccagtgtgc tcggtacggc cagaatggcc atctggattc agcagggggt gattgcggtt 8280 acatcatgta cggaaaaata attctaatcc ttgatttaaa tttgaacttg actatttatt 8340 tattctttat ttcattttgt aaatcatttt atgtatctcc tggcaagcaa ttttatccac 8400 cttgcaccaa caccttcggg ttccataatc aaaccacctt aacttcacac catgctgtaa 8460 ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa tttaataaac aatcatacga 8520 agcagtgaca aaataccaga tggtattaat gcttcgataa aattaattgg aaagtataaa 8580 atggtagaaa ataataaatt ataattaatt taagtaagat aaaaaataat taaaaactaa 8640 aatgttaaaa ttttaaaaaa attattttaa ataatattta aaaacattaa aaatcatttt 8700 aaaaaattta tttatagaac aattaaataa atatttcagc taataaaaaa caaaagctta 8760 cctagcctta gaagacaact tgtccaacaa ttagatgata cccattgccc ttacgttttc 8820 tttaacatca attattgttt ttgtcaacaa gctatctttt agttttattt tattggtaaa 8880 aaatatgtcg ccttcaagtt gcatcattta acacatctcg tcattagaaa aataaaactc 8940 ttccctaaac gattagtaga aaaaatcatt cgataataaa taagaaagaa aaattagaaa 9000 aaaataactt cattttaaaa aaatcattaa ggctatattt tttaaatgac taattttata 9060 tagactgtaa ctaaaagtat acaatttatt atgctatgta tcttaaagaa ttacttataa 9120 aaatctacgg aagaatatct tacaaagtga aaaacaaatg agaaagaatt tagtgggatg 9180 attatgattt tatttgaaaa ttgaaaaaat aattattaaa gactttagtg gagtaagaaa 9240 gctttcctat tagtcttttc ttatccataa aaaaaaaaaa aaaaatctag cgtgacagct 9300 tttccataga ttttaataat gtaaaatact ggtagcagcc gaccgttcag gtaatggaca 9360 ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat aacgccgtgg taacggataa 9420 agccaagcgt gaagcggtga aggtacatct ctgactccgt caagattacg aaaccgtcaa 9480 ctacgaagga ctccccgaaa tatcatctgt gtcataaaca ccaagtcaca ccatacatgg 9540 gcacgcgtca caatatgatt ggagaacggt tccaccgcat atgctataaa atgcccccac 9600 acccctcgac cctaatcgca cttcaattgc aatcaaatta gttcattctc tttgcgcagt 9660 tccctacctc tcctttcaag gttcgtagat ttcttccgtt tttttttctt cttctttatt 9720 gtttgttcta catcagcatg atgttgattt gattgtgttt tctatcgttt catcgattat 9780 aaattttcat aatcagaaga ttcagctttt attaatgcaa gaacgtcctt aattgatgat 9840 tttataaccg taaattaggt ctaattagag tttttttcat aaagattttc agatccgttt 9900 acaacaagcc ttaattgttg attctgtagt cgtagattaa ggtttttttc atgaactact 9960 tcagatccgt taaacaacag ccttatttgt tgatacttca gtcgtttttc aagaaattgt 10020 tcagatccgt tgataaaagc cttattcgtt gattctgtat ggtatttcaa gagatattgc 10080 tcaggtcctt tagcaactac cttatttgtt gattctgtgg ccatagatta ggattttttt 10140 tcacgaaatt gcttcttgaa attacgtgat ggattttgat tctgatttat cttgtgattg 10200 ttgactctac agccatggct gcaactactc ttacatctgc tttacctggt gctttcagtt 10260 catcccagag accaagtgct cctttcaact tacaaagatc acctagagtg cttagaaggt 10320 tcaacagaaa gacaggacgt caacctcgtg gacttgttcg tgctgcaaag gctcaagcgg 10380 cgcaaaacct gccagagaaa ttcgatgaaa caagactctg ggaggccctc aggggatttg 10440 gggttagtcc aagtagggtt acttacgctc ccgtcggatt cggagattac cactggaccg 10500 tcacagatga agacgggaga ccttggttcg caactgtcag tgacctcgaa cacaaagagc 10560 attgcgggca gggagcacaa gcagccctta aaggactcag acaagctatg gacactgccc 10620 tcgcacttag agatagggac ggactcagat tcgtggttgc tccagtggca gcaacagatg 10680 ggggtggccc tgtccttccc cttgacgcta ggtatgcact cacagtcttc cctcacgtcc 10740 caggaagaac aggagagttc ggacaaagac tcacagaggc agagagggac agactgcttg 10800 ctcttctggc agaactccac ggaagaacac ctcctgaaac aaccccacca gccgatatgg 10860 aaccaccagg acttcctggt gttagagcag cactcgccga aagtgaagga ccttggagtg 10920 gaggaccatt tgcagaaccc gctagacttc ttctcagaga acacgaggcc acactgcacg 10980 caagactcgc tgaattcgaa gccctcgtcg caagagttaa gggtcgtgga gcacctcttg 11040 tcgttaccca cggagaacca cacccaggca acttgatcct cggagaggaa ggctatctcc 11100 tcgtggactg ggatacagtt ggactggctc cagctgaaag ggatttgagt ctcatctcag 11160 acgacccagc tgacctcgca agatacgcag agcttacagg aagaactcca gacccagacg 11220 cactcgcact ctatagactg cgttggagtc tccttgatgt ggctgagttc gtcgagtggt 11280 tcagggctga acaccaaaga acccaagaca ccgagagtgc atggaagggg ttcactgaca 11340 cacttgggca attggctgct ggtggatcag ctgcatagca aacttatctc tgttatgaat 11400 cagaagaagt tcatgtctcg tttcatttaa aactttggtg gtttgtgttt tggggccttg 11460 taaagcccct gatgaataat tgttcaacta tgtttccgtt cctgtgttat acctttcttt 11520 ctaatgagta atgacatcaa acttcttctg tattgaaatt atgtccttgt gagtctcttt 11580 atcatcgttt cgtctttaca ttatatgtgc tacttttgtc taatgagcct gaaaagtggc 11640 tccaatggta cgcactggaa gatttgttgg cttctggtag atatagcgac agtgttgagc 11700 ttgtaatatc atgtctctta ttgctaaatt agttcctttc ttaacagaaa ccttcaaagt 11760 ttttgttttt gttttcattt acctaatgta cacatacgct ggccatgact aacaacatgt 11820 ccaggcttag agcatatttt tttctagctt aaattgttaa cttgtcattc agtaaaatcc 11880 gagaattgtg aagctctaat tgaagctaat tcgttttata aagtcagtta aaaagtatac 11940 taaattatcc aacttttctt caaaatctca aaattctatg acaaaacgat agtctttgtt 12000 tatgtcagta ccacaaagag gtggaaaaaa acaccaaaaa aacaataagc aaactataca 12060 ctgagaagaa aaataaaaga gagctcaata gatgttttat actaacggta gattagatca 12120 aagatccaag ctttactcta catagagcag aacccagaat cccttcatat ctcttttatt 12180 ctagcaccga taatctactg aaaagaagac acttagagct ctgtctcttt gtcaaagaag 12240 tcccagccgt catccagaag ctccttacgt tcattaacag aggccgcaat tggatcgggt 12300 ttacttattt tgtgggtatc tatactttta ttagattttt aatcaggctc ctgatttctt 12360 tttatttcga ttgaattcct gaacttgtat tattcagtag atcgaataaa ttataaaaag 12420 ataaaatcat aaaataatat tttatcctat caatcatatt aaagcaatga atatgtaaaa 12480 ttaatcttat ctttatttta aaaaatcata taggtttagt atttttttaa aaataaagat 12540 aggattagtt ttactattca ctgcttatta cttttaaaaa aatcataaag gtttagtatt 12600 tttttaaaat aaatatagga atagttttac tattcactgc tttaatagaa aaatagttta 12660 aaatttaaga tagttttaat cccagcattt gccacgtttg aacgtgagcc gaaacgatgt 12720 cgttacatta tcttaaccta gctgaaacga tgtcgtcata atatcgccaa atgccaactg 12780 gactacgtcg aacccacaaa tcccacaaag cgcgtgaaat caaatcgctc aaaccacaaa 12840 aaagaacaac gcgtttgtta cacgctcaat cccacgcgag tagagcacag taaccttcaa 12900 ataagcgaat ggggcataat cagaaatccg aaataaacct aggggcatta tcggaaatga 12960 aaagtagctc actcaatata aaaatctagg aaccctagtt ttcgttatca ctctgtgctc 13020 cctcgctcta tttctcagtc tctgtgtttg cggctgagga ttccgaacga gtgaccttct 13080 tcgtttctcg caaaggtaac agcctctgct cttgtctctt cgattcgatc tatgcctgtc 13140 tcttatttac gatgatgttt cttcggttat gtttttttat ttatgcttta tgctgttgat 13200 gttcggttgt ttgtttcgct ttgtttttgt ggttcagttt tttaggattc ttttggtttt 13260 tgaatcgatt aatcggaaga gattttcgag ttatttggtg tgttggaggt gaatcttttt 13320 tttgaggtca tagatctgtt gtatttgtgt tataaacatg cgactttgta tgatttttta 13380 cgaggttatg atgttctggt tgttttatta tgaatctgtt gagacagaac catgattttt 13440 gttgatgttc gtttacacta ttaaaggttt gttttaacag gattaaaagt tttttaagca 13500 tgttgaagga gtcttgtaga tatgtaaccg tcgatagttt ttttgtgggt ttgttcacat 13560 gttatcaagc ttaatctttt actatgtatg cgaccatatc tggatccagc aaaggcgatt 13620 ttttaattcc ttgtgaaact tttgtaatat gaagttgaaa ttttgttatt ggtaaactat 13680 aaatgtgtga agttggagta tacctttacc ttcttatttg gctttgtgat agtttaattt 13740 atatgtattt tgagttctga cttgtatttc tttgaattga ttctagttta agtaatcatg 13800 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc 13860 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac 13920 aacaccgtga agctgaaggt gacgaagggc ggccccctgc ccttcgcctg ggacatcctg 13980 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac 14040 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 14100 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag 14160 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg 14220 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc 14280 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac 14340 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc 14400 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac 14460 cacctgttcc tgtagttttg tgatctgatg ataagtggtt ggttcgtgtc tcatgcactt 14520 gggaggtgat ctatttcacc tggtgtagtt tgtgtttccg tcagttggaa aaacttatcc 14580 ctatcgattt cgttttcatt ttctgctttt cttttatgta ccttcgtttg ggcttgtaac 14640 gggcctttgt atttcaactc tcaataataa tccaagtgca tgttaaacaa tttgtcatct 14700 gtttcggctt tgatatacta ctggtgaaga tgggccgtac tactgcatca caacgaaaaa 14760 taataataag atgaaaaact tgaagtggaa aaaaaaaaaa acttgaatgt tcactactac 14820 tcattgacca taatgtttaa catacatagc tcaatagtat ttttgtgaat atggcaacac 14880 aaacagtcca aaacaattgt ctcttactat accaaaccaa gggcgccgct tgtttgccac 14940 tctttgtgtg caatagtgtg attaccacat ctccacattc aatatattcc ctgaattatc 15000 tgacgatttt gatggctcac tgttttccca agtcttgaat tgtcttctgt gcgccagtca 15060 aatgcatatg tgttgagttt atcttttaaa tatcaagctt ttgtttttaa cttttgtttg 15120 taaccaaaaa ctcacagtag gagtttgatc acataatttt atgtttgcct ttgcaatttc 15180 tagtgagtct ttgattaaaa gcttgaaaag aaaatgcagc caagcttacc aagtaagtta 15240 tgtgtattaa ccagaggaag agagaatctt gcaaaatttc aacaaacaca aaaagaagta 15300 ttactacgat tggtggagaa agaaaacgat tccaaatctt gaactgttgt tgtaaaagca 15360 tagcagaaag tgggagacaa ccgaaataga aatgactata acttaattta atgttatcat 15420 tataatttct tctagcaaat atttagaaag taaatatcac atcaaccttt aatgtaatta 15480 agctttctct ttttgattca tgtgagatga aaagaaaaaa aagaagagaa aagtgtagaa 15540 aacacatcat ttctaagctg aagctgaggt acaaattaat taataagtga ctagggtcac 15600 gtgaccctag tcacttatga ttccgatgac ttcgtaggtt cctagctcaa gccgctcgtg 15660 tccaagcgtc acttacgatt agctaatgat tacggcatct aggaccgact agttcttatg 15720 tgcttctagt ctccaaatgt ggttgatagt tattttgctc taagatcaac agtaatgaag 15780 tataaatcat cgttgtggtg tgctactcgg ttaattgagc attaacacac acaaacatga 15840 cgaggatggt ataatctcca aaaatgtgta ctttgttagg tgggacccta tagccttgat 15900 taatgtgcta tgttaggcat gcctggaaac gtgtgacgca tatgttttgt gaacctgttg 15960 atattatatg tgcttttata ttaccatatt ttattaaaat actaatattt attactagta 16020 agatataaca ttctatctag cttaaaaact aaccataaat attccataat aactagattt 16080 accaaactaa tatactaaat atacataata aatacaaaat taacaagaca ataatcaata 16140 tttatgagct taatatattt agacattatg gttggtcgac gataatcatg ctaacttttc 16200 gtaattgctt gattgaaata tgcttagaat aatgcctctt tgttctacat ggcaaatagg 16260 gaccattatg gtgtaacacc ctgggaacca caaacacccc gaaatgctac taaactacac 16320 aactaacctt catatataaa atttcgacag catctccttt gaaaatttgc atagacgtgg 16380 aagcaacaga gtataaacag atctagctag ctaagtttaa acggccggcc aaacgtgcaa 16440 gcgctactag acaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag 16500 cgtcaattta tttacaattg aatatatcct gcca 16534 <210> 174 <211> 16534 <212> DNA <213> Artificial Sequence <220> <223> RV027342 T-DNA <400> 174 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgctttat tttatatgtt 1440 taattttata gaatctaact agcttatttt gagattaaat tgtttagtta cttttataac 1500 agtataaatg tataatgagg acctaagaat gtagtcctgt aatgttcttg ctattctact 1560 taatctcatc accaatcaac catcaaaaga agctagtact aataaaacct gcaggtattc 1620 gaataataat taagctcaaa cactatacta atttatggag gattatatat tcaatgaatt 1680 aggaacctca tgatggacat tattgactga tataatgtgt atactaattg tgagtattta 1740 aaaaccatac aaagcattta tatgtccaca tatattggac acacatgcaa tcaatgttca 1800 atatgctcca cacacagaaa taaaaatact ctttctgatc atatgataca tcatacatat 1860 actaaaaaaa tctaaaatga actataacca caagcatata taataacaat gaaatggtaa 1920 tgtttcttca tttttatttg ttcaaattct tattcggttg ttttttctta ccctacgaga 1980 atccgtgagg tcaaagggaa acagtgattt tttttttgta ttttgttttt taaattgatg 2040 aactgtaaaa ctctctctct agaaaaatat ataagtagta gtatgaattt tctctcacta 2100 aaagcattaa tggacctttc gataatcata aatgcaatgc accctctcta tgcatttcgc 2160 aataactcct tttccttctg ccacatcctc ttcctcacct ctttctcttc ttccctttct 2220 cctaagttcc tcctccacca aattctccat ttatttcgtt aactatcctc catttgtttt 2280 cttctgaaga gtgatatatt ctacctttct ctggttaaag aaactccctg aatccaccgg 2340 ttatgtcttg accggctata agcctataaa ctgatgccct aagacacctt tttaggtttc 2400 tcaataattc tccgcatcta tcttttcttc tccacaagta agagaaccag aaaaccagag 2460 aagaagccga gctagctagg gtttcattgt gtgcacaaaa gtaagatctc tctctctaac 2520 caatacttgt gtaatttgtc tttgtttctt tgagcaaata ttgcatgttt gttcatatta 2580 gccggatccg ttttatattt tttcatgatc tacattttat ctttattttg tttgtaaatt 2640 aatgagtttt tttttttttt ttctgttttt gtcacgatct aaaaaacaag cgttacaaag 2700 aagaagaaaa acctttttgg agttagaagt gtaaaagggg tttcagtttg acgaattttc 2760 cttagtagtt gtgtaaaaaa aggccattga cttaatgtca actctatata tctacacatt 2820 tttttattaa ttagtttttg tttttttccc acttcattta cctttagtca atgaattttt 2880 actgaaaacg ttttttcaag gtcaatttca ctgagttaaa aaaaaccatg gaaactcaac 2940 aacaacaagc agatattcaa gattttggga acaaaaacag taacacctat gcatgcaggc 3000 aaagcagtac taggtggaca cccacaagtg atcagatcag aatactgaaa gagctctact 3060 acaacaatgg aatcagatca ccaactgctg atcagattca gagaatcgct gctcaactca 3120 gacagtacgg caagattgaa ggcaagaacg tgttttattg gtttcagaac cataaagctc 3180 gtgaacgcca gaagaaacgg ttcactccta ctgctcctcc accaccgtct cacttctccg 3240 atcatattaa ccaccatctt cccaacgctg cccccatgca gattccgtct caccaccacc 3300 actaccacca tcaagaacca ccacatgtct atgctcaccc tcacaaactc tacaccactc 3360 atcacattgg ggttggttct tcttcacaag gagtgatggg tgtagggtgt ggctatggat 3420 ctgttgctat ggagaagagt ttcaggaagt gttcaatatc accaccggga gagagtaagg 3480 ccacaggagg aattggccgg aatatcggtt caagatcacg gataagtgtc gattcgtgtt 3540 ccttcttcga cacaattaag ccaaagacat atgagatgtt tgagaatcat gatcaagatg 3600 aagaacaagg agagccttca actgagatcg aaacacttcc attattcccg attcacgacg 3660 gcaaccatca tgatttcttc ggcatgagga cggcaaatct gcaattggag caaggcactg 3720 gaggttacta cactggagga agctggtatc ggtctgatga gagggcgtct cttgagctca 3780 gtctcaactc atatggttat tataactaat tcgaactggc caggtacctg acatcttata 3840 gtctgcaacc tctcgtgtct gaattcctat ctttatcaag tgttattgct tccacgacta 3900 taggacagct ttcgtcgaaa ggttttgctc atgtgatctc gaaggattca tctagtctga 3960 tttttcgtga cttgtatcgg ttttattgga ttcatccaac atatatcaat aaaaaatgag 4020 ttgtgtttcc tttcttccta gttcagttaa aattatttcc ctcctgcgct tgtgctgtaa 4080 ttgtctgtgt acctgttgtt tgtgactgtg ttagttccct tggatatgat ttcgtatttg 4140 atatgtacat ggagatagct tagcttcatt attggagtat gaagttagta tgacatagtc 4200 actctcctgg aaaattgaca ctgcaaacca tatttttatt ctgaaccaca aatcctagtc 4260 agtccgctgg catatgccgt ccgtttgctg aatccagaac gtgggtttgg agatgtacgg 4320 ctgagatgcc tctatgcgaa ggggatttcg tggtgaaacg agatgggagt agagcaacgc 4380 ccgtggaaga tgcttcaaac ttccacactt ttgagcaacg atcggcagta gtaaggtaga 4440 cgatttcaag atcaaagcat atgaagataa acaacatcaa caacaaaatt tgttggggtt 4500 ctatagagag aaacagagct acatacatac actgttttgt atctaccatc tgagatgatg 4560 aaaagatgaa aaactaaaga atgccccggc gccaacgcca ggacacgccg cgcgcgcgtc 4620 acccgagcca tctcttgacc cagccggcgc tgtatattta cacacgttgc agcatcgatc 4680 accacctgtt cgatcgcgtc gccgtcacca agcttggcgc gccggtcacc cggtccgggc 4740 ctagaaggcc acccggggct cggcgttagg cttttagtcc gctaggtggc tagaccgagg 4800 ttaggacgct agttcactag gggcctagtg gtgttttaag gatgggcaag tttgtacaaa 4860 aaagcaggct ggcgccggaa ccaattcagt cgagtgcggc cgccagtgtg atggatatct 4920 cgacccgggt gattgcggtt acatcatgta cggaaaaata attctaatcc ttgatttaaa 4980 tttgaacttg actatttatt tattctttat ttcattttgt aaatcatttt atgtatctcc 5040 tggcaagcaa ttttatccac cttgcaccaa caccttcggg ttccataatc aaaccacctt 5100 aacttcacac catgctgtaa ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa 5160 tttaataaac aatcatacga agcagtgaca aaataccaga tggtattaat gcttcgataa 5220 aattaattgg aaagtataaa atggtagaaa ataataaatt ataattaatt taagtaagat 5280 aaaaaataat taaaaactaa aatgttaaaa ttttaaaaaa attattttaa ataatattta 5340 aaaacattaa aaatcatttt aaaaaattta tttatagaac aattaaataa atatttcagc 5400 taataaaaaa caaaagctta cctagcctta gaagacaact tgtccaacaa ttagatgata 5460 cccattgccc ttacgttttc tttaacatca attattgttt ttgtcaacaa gctatctttt 5520 agttttattt tattggtaaa aaatatgtcg ccttcaagtt gcatcattta acacatctcg 5580 tcattagaaa aataaaactc ttccctaaac gattagtaga aaaaatcatt cgataataaa 5640 taagaaagaa aaattagaaa aaaataactt cattttaaaa aaatcattaa ggctatattt 5700 tttaaatgac taattttata tagactgtaa ctaaaagtat acaatttatt atgctatgta 5760 tcttaaagaa ttacttataa aaatctacgg aagaatatct tacaaagtga aaaacaaatg 5820 agaaagaatt tagtgggatg attatgattt tatttgaaaa ttgaaaaaat aattattaaa 5880 gactttagtg gagtaagaaa gctttcctat tagtcttttc ttatccataa aaaaaaaaaa 5940 aaaaatctag cgtgacagct tttccataga ttttaataat gtaaaatact ggtagcagcc 6000 gaccgttcag gtaatggaca ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat 6060 aacgccgtgg taacggataa agccaagcgt gaagcggtga aggtacatct ctgactccgt 6120 caagattacg aaaccgtcaa ctacgaagga ctccccgaaa tatcatctgt gtcataaaca 6180 ccaagtcaca ccatacatgg gcacgcgtca caatatgatt ggagaacggt tccaccgcat 6240 atgctataaa atgcccccac acccctcgac cctaatcgca cttcaattgc aatcaaatta 6300 gttcattctc tttgcgcagt tccctacctc tcctttcaag gttcgtagat ttcttccgtt 6360 tttttttctt cttctttatt gtttgttcta catcagcatg atgttgattt gattgtgttt 6420 tctatcgttt catcgattat aaattttcat aatcagaaga ttcagctttt attaatgcaa 6480 gaacgtcctt aattgatgat tttataaccg taaattaggt ctaattagag tttttttcat 6540 aaagattttc agatccgttt acaacaagcc ttaattgttg attctgtagt cgtagattaa 6600 ggtttttttc atgaactact tcagatccgt taaacaacag ccttatttgt tgatacttca 6660 gtcgtttttc aagaaattgt tcagatccgt tgataaaagc cttattcgtt gattctgtat 6720 ggtatttcaa gagatattgc tcaggtcctt tagcaactac cttatttgtt gattctgtgg 6780 ccatagatta ggattttttt tcacgaaatt gcttcttgaa attacgtgat ggattttgat 6840 tctgatttat cttgtgattg ttgactctac agccatggcc cacagcaagc acggcctgaa 6900 ggaggagatg accatgaagt accacatgga gggctgcgtg aacggccaca agttcgtgat 6960 caccggcgag ggcatcggct accccttcaa gggcaagcag accatcaacc tgtgcgtgat 7020 cgagggcggc cccctgccct tcagcgagga catcctgagc gccggcttca agtacggcga 7080 ccggatcttc accgagtacc cccaggacat cgtggactac ttcaagaaca gctgccccgc 7140 cggctacacc tggggccgga gcttcctgtt cgaggacggc gccgtgtgca tctgtaacgt 7200 ggacatcacc gtgagcgtga aggagaactg catctaccac aagagcatct tcaacggcgt 7260 gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc accaactggg aggccagctg 7320 cgagaagatc atgcccgtgc ctaagcaggg catcctgaag ggcgacgtga gcatgtacct 7380 gctgctgaag gacggcggcc ggtaccggtg ccagttcgac accgtgtaca aggccaagag 7440 cgtgcccagc aagatgcccg agtggcactt catccagcac aagctgctgc gggaggaccg 7500 gagcgacgcc aagaaccaga agtggcagct gaccgagcac gccatcgcct tccccagcgc 7560 cctggcctga gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa 7620 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta 7680 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta 7740 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg 7800 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattctag tggccggccc 7860 agctgatatc tagacccagc tttcttgtac aaagtggccg ttaacggatc ggccagaatg 7920 gcccggaccg ggttaccgaa ttcgagctcg gtaccctggg atccgatatc gatgggccct 7980 ggccgaagct tggtcacccg gtccgggcct agaaggccag cttcggccgc cccgggcaac 8040 tttattatac aaagttgatg gtacggatcc gtcgacggcg cgccgcggac ctgaattcca 8100 gcacactggc ggccgttagt ctaaaaacgg cccctaacta acgcttagtt gcccgctaga 8160 ttagtccccc gttagtgcct agttagacac acgacgaact aggggctact agtaacggcc 8220 gccagtgtgc tcggtacggc cagaatggcc atctggattc agcagggggt gattgcggtt 8280 acatcatgta cggaaaaata attctaatcc ttgatttaaa tttgaacttg actatttatt 8340 tattctttat ttcattttgt aaatcatttt atgtatctcc tggcaagcaa ttttatccac 8400 cttgcaccaa caccttcggg ttccataatc aaaccacctt aacttcacac catgctgtaa 8460 ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa tttaataaac aatcatacga 8520 agcagtgaca aaataccaga tggtattaat gcttcgataa aattaattgg aaagtataaa 8580 atggtagaaa ataataaatt ataattaatt taagtaagat aaaaaataat taaaaactaa 8640 aatgttaaaa ttttaaaaaa attattttaa ataatattta aaaacattaa aaatcatttt 8700 aaaaaattta tttatagaac aattaaataa atatttcagc taataaaaaa caaaagctta 8760 cctagcctta gaagacaact tgtccaacaa ttagatgata cccattgccc ttacgttttc 8820 tttaacatca attattgttt ttgtcaacaa gctatctttt agttttattt tattggtaaa 8880 aaatatgtcg ccttcaagtt gcatcattta acacatctcg tcattagaaa aataaaactc 8940 ttccctaaac gattagtaga aaaaatcatt cgataataaa taagaaagaa aaattagaaa 9000 aaaataactt cattttaaaa aaatcattaa ggctatattt tttaaatgac taattttata 9060 tagactgtaa ctaaaagtat acaatttatt atgctatgta tcttaaagaa ttacttataa 9120 aaatctacgg aagaatatct tacaaagtga aaaacaaatg agaaagaatt tagtgggatg 9180 attatgattt tatttgaaaa ttgaaaaaat aattattaaa gactttagtg gagtaagaaa 9240 gctttcctat tagtcttttc ttatccataa aaaaaaaaaa aaaaatctag cgtgacagct 9300 tttccataga ttttaataat gtaaaatact ggtagcagcc gaccgttcag gtaatggaca 9360 ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat aacgccgtgg taacggataa 9420 agccaagcgt gaagcggtga aggtacatct ctgactccgt caagattacg aaaccgtcaa 9480 ctacgaagga ctccccgaaa tatcatctgt gtcataaaca ccaagtcaca ccatacatgg 9540 gcacgcgtca caatatgatt ggagaacggt tccaccgcat atgctataaa atgcccccac 9600 acccctcgac cctaatcgca cttcaattgc aatcaaatta gttcattctc tttgcgcagt 9660 tccctacctc tcctttcaag gttcgtagat ttcttccgtt tttttttctt cttctttatt 9720 gtttgttcta catcagcatg atgttgattt gattgtgttt tctatcgttt catcgattat 9780 aaattttcat aatcagaaga ttcagctttt attaatgcaa gaacgtcctt aattgatgat 9840 tttataaccg taaattaggt ctaattagag tttttttcat aaagattttc agatccgttt 9900 acaacaagcc ttaattgttg attctgtagt cgtagattaa ggtttttttc atgaactact 9960 tcagatccgt taaacaacag ccttatttgt tgatacttca gtcgtttttc aagaaattgt 10020 tcagatccgt tgataaaagc cttattcgtt gattctgtat ggtatttcaa gagatattgc 10080 tcaggtcctt tagcaactac cttatttgtt gattctgtgg ccatagatta ggattttttt 10140 tcacgaaatt gcttcttgaa attacgtgat ggattttgat tctgatttat cttgtgattg 10200 ttgactctac agccatggct gcaactactc ttacatctgc tttacctggt gctttcagtt 10260 catcccagag accaagtgct cctttcaact tacaaagatc acctagagtg cttagaaggt 10320 tcaacagaaa gacaggacgt caacctcgtg gacttgttcg tgctgcaaag gctcaagcgg 10380 cgcaaaacct gccagagaaa ttcgatgaaa caagactctg ggaggccctc aggggatttg 10440 gggttagtcc aagtagggtt acttacgctc ccgtcggatt cggagattac cactggaccg 10500 tcacagatga agacgggaga ccttggttcg caactgtcag tgacctcgaa cacaaagagc 10560 attgcgggca gggagcacaa gcagccctta aaggactcag acaagctatg gacactgccc 10620 tcgcacttag agatagggac ggactcagat tcgtggttgc tccagtggca gcaacagatg 10680 ggggtggccc tgtccttccc cttgacgcta ggtatgcact cacagtcttc cctcacgtcc 10740 caggaagaac aggagagttc ggacaaagac tcacagaggc agagagggac agactgcttg 10800 ctcttctggc agaactccac ggaagaacac ctcctgaaac aaccccacca gccgatatgg 10860 aaccaccagg acttcctggt gttagagcag cactcgccga aagtgaagga ccttggagtg 10920 gaggaccatt tgcagaaccc gctagacttc ttctcagaga acacgaggcc acactgcacg 10980 caagactcgc tgaattcgaa gccctcgtcg caagagttaa gggtcgtgga gcacctcttg 11040 tcgttaccca cggagaacca cacccaggca acttgatcct cggagaggaa ggctatctcc 11100 tcgtggactg ggatacagtt ggactggctc cagctgaaag ggatttgagt ctcatctcag 11160 acgacccagc tgacctcgca agatacgcag agcttacagg aagaactcca gacccagacg 11220 cactcgcact ctatagactg cgttggagtc tccttgatgt ggctgagttc gtcgagtggt 11280 tcagggctga acaccaaaga acccaagaca ccgagagtgc atggaagggg ttcactgaca 11340 cacttgggca attggctgct ggtggatcag ctgcatagca aacttatctc tgttatgaat 11400 cagaagaagt tcatgtctcg tttcatttaa aactttggtg gtttgtgttt tggggccttg 11460 taaagcccct gatgaataat tgttcaacta tgtttccgtt cctgtgttat acctttcttt 11520 ctaatgagta atgacatcaa acttcttctg tattgaaatt atgtccttgt gagtctcttt 11580 atcatcgttt cgtctttaca ttatatgtgc tacttttgtc taatgagcct gaaaagtggc 11640 tccaatggta cgcactggaa gatttgttgg cttctggtag atatagcgac agtgttgagc 11700 ttgtaatatc atgtctctta ttgctaaatt agttcctttc ttaacagaaa ccttcaaagt 11760 ttttgttttt gttttcattt acctaatgta cacatacgct ggccatgact aacaacatgt 11820 ccaggcttag agcatatttt tttctagctt aaattgttaa cttgtcattc agtaaaatcc 11880 gagaattgtg aagctctaat tgaagctaat tcgttttata aagtcagtta aaaagtatac 11940 taaattatcc aacttttctt caaaatctca aaattctatg acaaaacgat agtctttgtt 12000 tatgtcagta ccacaaagag gtggaaaaaa acaccaaaaa aacaataagc aaactataca 12060 ctgagaagaa aaataaaaga gagctcaata gatgttttat actaacggta gattagatca 12120 aagatccaag ctttactcta catagagcag aacccagaat cccttcatat ctcttttatt 12180 ctagcaccga taatctactg aaaagaagac acttagagct ctgtctcttt gtcaaagaag 12240 tcccagccgt catccagaag ctccttacgt tcattaacag aggccgcaat tggatcgggt 12300 ttacttattt tgtgggtatc tatactttta ttagattttt aatcaggctc ctgatttctt 12360 tttatttcga ttgaattcct gaacttgtat tattcagtag atcgaataaa ttataaaaag 12420 ataaaatcat aaaataatat tttatcctat caatcatatt aaagcaatga atatgtaaaa 12480 ttaatcttat ctttatttta aaaaatcata taggtttagt atttttttaa aaataaagat 12540 aggattagtt ttactattca ctgcttatta cttttaaaaa aatcataaag gtttagtatt 12600 tttttaaaat aaatatagga atagttttac tattcactgc tttaatagaa aaatagttta 12660 aaatttaaga tagttttaat cccagcattt gccacgtttg aacgtgagcc gaaacgatgt 12720 cgttacatta tcttaaccta gctgaaacga tgtcgtcata atatcgccaa atgccaactg 12780 gactacgtcg aacccacaaa tcccacaaag cgcgtgaaat caaatcgctc aaaccacaaa 12840 aaagaacaac gcgtttgtta cacgctcaat cccacgcgag tagagcacag taaccttcaa 12900 ataagcgaat ggggcataat cagaaatccg aaataaacct aggggcatta tcggaaatga 12960 aaagtagctc actcaatata aaaatctagg aaccctagtt ttcgttatca ctctgtgctc 13020 cctcgctcta tttctcagtc tctgtgtttg cggctgagga ttccgaacga gtgaccttct 13080 tcgtttctcg caaaggtaac agcctctgct cttgtctctt cgattcgatc tatgcctgtc 13140 tcttatttac gatgatgttt cttcggttat gtttttttat ttatgcttta tgctgttgat 13200 gttcggttgt ttgtttcgct ttgtttttgt ggttcagttt tttaggattc ttttggtttt 13260 tgaatcgatt aatcggaaga gattttcgag ttatttggtg tgttggaggt gaatcttttt 13320 tttgaggtca tagatctgtt gtatttgtgt tataaacatg cgactttgta tgatttttta 13380 cgaggttatg atgttctggt tgttttatta tgaatctgtt gagacagaac catgattttt 13440 gttgatgttc gtttacacta ttaaaggttt gttttaacag gattaaaagt tttttaagca 13500 tgttgaagga gtcttgtaga tatgtaaccg tcgatagttt ttttgtgggt ttgttcacat 13560 gttatcaagc ttaatctttt actatgtatg cgaccatatc tggatccagc aaaggcgatt 13620 ttttaattcc ttgtgaaact tttgtaatat gaagttgaaa ttttgttatt ggtaaactat 13680 aaatgtgtga agttggagta tacctttacc ttcttatttg gctttgtgat agtttaattt 13740 atatgtattt tgagttctga cttgtatttc tttgaattga ttctagttta agtaatcatg 13800 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc 13860 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac 13920 aacaccgtga agctgaaggt gacgaagggc ggccccctgc ccttcgcctg ggacatcctg 13980 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac 14040 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 14100 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag 14160 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg 14220 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc 14280 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac 14340 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc 14400 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac 14460 cacctgttcc tgtagttttg tgatctgatg ataagtggtt ggttcgtgtc tcatgcactt 14520 gggaggtgat ctatttcacc tggtgtagtt tgtgtttccg tcagttggaa aaacttatcc 14580 ctatcgattt cgttttcatt ttctgctttt cttttatgta ccttcgtttg ggcttgtaac 14640 gggcctttgt atttcaactc tcaataataa tccaagtgca tgttaaacaa tttgtcatct 14700 gtttcggctt tgatatacta ctggtgaaga tgggccgtac tactgcatca caacgaaaaa 14760 taataataag atgaaaaact tgaagtggaa aaaaaaaaaa acttgaatgt tcactactac 14820 tcattgacca taatgtttaa catacatagc tcaatagtat ttttgtgaat atggcaacac 14880 aaacagtcca aaacaattgt ctcttactat accaaaccaa gggcgccgct tgtttgccac 14940 tctttgtgtg caatagtgtg attaccacat ctccacattc aatatattcc ctgaattatc 15000 tgacgatttt gatggctcac tgttttccca agtcttgaat tgtcttctgt gcgccagtca 15060 aatgcatatg tgttgagttt atcttttaaa tatcaagctt ttgtttttaa cttttgtttg 15120 taaccaaaaa ctcacagtag gagtttgatc acataatttt atgtttgcct ttgcaatttc 15180 tagtgagtct ttgattaaaa gcttgaaaag aaaatgcagc caagcttacc aagtaagtta 15240 tgtgtattaa ccagaggaag agagaatctt gcaaaatttc aacaaacaca aaaagaagta 15300 ttactacgat tggtggagaa agaaaacgat tccaaatctt gaactgttgt tgtaaaagca 15360 tagcagaaag tgggagacaa ccgaaataga aatgactata acttaattta atgttatcat 15420 tataatttct tctagcaaat atttagaaag taaatatcac atcaaccttt aatgtaatta 15480 agctttctct ttttgattca tgtgagatga aaagaaaaaa aagaagagaa aagtgtagaa 15540 aacacatcat ttctaagctg aagctgaggt acaaattaat taataagtga ctagggtcac 15600 gtgaccctag tcacttatga ttccgatgac ttcgtaggtt cctagctcaa gccgctcgtg 15660 tccaagcgtc acttacgatt agctaatgat tacggcatct aggaccgact agttcttatg 15720 tgcttctagt ctccaaatgt ggttgatagt tattttgctc taagatcaac agtaatgaag 15780 tataaatcat cgttgtggtg tgctactcgg ttaattgagc attaacacac acaaacatga 15840 cgaggatggt ataatctcca aaaatgtgta ctttgttagg tgggacccta tagccttgat 15900 taatgtgcta tgttaggcat gcctggaaac gtgtgacgca tatgttttgt gaacctgttg 15960 atattatatg tgcttttata ttaccatatt ttattaaaat actaatattt attactagta 16020 agatataaca ttctatctag cttaaaaact aaccataaat attccataat aactagattt 16080 accaaactaa tatactaaat atacataata aatacaaaat taacaagaca ataatcaata 16140 tttatgagct taatatattt agacattatg gttggtcgac gataatcatg ctaacttttc 16200 gtaattgctt gattgaaata tgcttagaat aatgcctctt tgttctacat ggcaaatagg 16260 gaccattatg gtgtaacacc ctgggaacca caaacacccc gaaatgctac taaactacac 16320 aactaacctt catatataaa atttcgacag catctccttt gaaaatttgc atagacgtgg 16380 aagcaacaga gtataaacag atctagctag ctaagtttaa acggccggcc aaacgtgcaa 16440 gcgctactag acaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag 16500 cgtcaattta tttacaattg aatatatcct gcca 16534 <210> 175 <211> 16534 <212> DNA <213> Artificial Sequence <220> <223> RV027343 T-DNA <400> 175 caaacaaaca tacacagcga tttattcaca cgaacccgaa ttacaacggt atatatcctg 60 tcagccaaca ccaaaacatc aaaaaaatcg ggatcaggat caggatctgc ggccgcatcc 120 tgcaggttta aacggccggc cttcactagc tagctgctaa gctcaaacga gcaggaagca 180 acgagagggt ggcgcgcgac cgacgtgcgt acgtagcatg agcctgagtg gagacgttgg 240 acgtgtatgt atatacctct ctgcgtgtta actatgtacg taagcggcag gcagtgcaat 300 aagtgtggct ctgtagtatg tacgtgcggg tacgatgctg taagctactg aggcaagtcc 360 ataaataaat aatgacacgt gcgtgttcta taatctcttc gcttcttcat ttgtcccctt 420 gcggagtttg gcatccattg atgccgttac gctgagaaca gacacagcag acgaaccaaa 480 agtgagttct tgtatgaaac tatgaccctt catcgctagg ctcaaacagc accccgtacg 540 aacacagcaa attagtcatc taactattag cccctacatg tttcagacga tacataaata 600 tagcccatcc ttagcaatta gctattggcc ctgcccatcc caagcaatga tctcgaagta 660 tttttaatat atagtatttt taatatgtag cttttaaaat tagaagataa ttttgagaca 720 aaaatctcca agtatttttt tgggtatttt ttactgcctc cgtttttctt tatttctcgt 780 cacctagttt aattttgtgc taatcggcta taaacgaaac agagagaaaa gttactctaa 840 aagcaactcc aacagattag atataaatct tatatcctgc ctagagctgt taaaaagata 900 gacaacttta gtggattagt gtatgcaaca aactctccaa atttaagtat cccaactacc 960 caacgcatat cgttcccttt tcattggcgc acgaactttc acctgctata gccgacgtac 1020 atgttcgttt tttttgggcg gcgcttactt tcttccccgt tcgttctcag catcgcaact 1080 caatttgtta tggcggagaa gcccttgtat cccaggtagt aatgcacaga tatgcattat 1140 tattattcat aaaatcgagc tagttaccct atgaggtgac atgaagcgct cacggttact 1200 atgacggtta gcttcacgac tgttggtggc agtagcgtac gacttagcta tagttccgga 1260 cttacctaac tgactagagt cacacttagc tgaccctagt cacttaggcg cgccaagctt 1320 ggatctcctg caggatctgg ccggccggat gggcggatct cgtacctcaa ctttgtatag 1380 aaaagttggg ccagaatggc ccggaccggg ttaccaagcg gccgctggca acttcaatga 1440 attctgaatt taaaacattt ttttttatat atatagatga tttggcgatt ttcgaactgg 1500 aatggtgaaa aagtggttga gtacactatt agtttattta tttaaaaaat agagggaaat 1560 tcaaatgaaa aaaaaagttt catctacaga aaaaaaaaca cgtcaaaatt ccaaaaaact 1620 tgaacgtctt gaacaacggt ataaccgtat aagaaaggga acttaaaact taaacgtcta 1680 catgattgaa agggtaaaaa tattcatatt acaatggtat aagaaaatgg agaagccaga 1740 ggagcaactc caaagaagaa gatgcagcaa gagagtgtac attattataa ttataagttg 1800 gtatatatgt agttgctaaa aaaaaaaaag agtgtacaat attttctgaa gaagcatcga 1860 gctggttaat atttaagacc caccaaaacc taccaacaat gttattctca tctctaagca 1920 aaagtttgat ctcatctttt tttttaattt tttttttgat gggtcctctt ctacctctgc 1980 atctccacgt gctatacaag tggcctcgct gtatgtatta tttaccactc tcttattgtt 2040 actaaaaact tgaatgtcac ttgaatgtca cttgaatgca aactatatat ttaggtgaat 2100 accagatcaa atagcaataa ataaaccaac aatttatgct aattaaaaac ttgaaaaaac 2160 actttccatt acctatgatt ttttaatcca atcttggggt ttgatgccca taaaacgaag 2220 tcgaaaagag aaaagcgacc ttaattgtaa taaatgcagc tattgacttc tctatcaact 2280 tcaagtcttc aagtaactac aaatttaata atctccttag cttatcttag agaagcacaa 2340 aacaaaaggg tctacgctta gtcttcctag attcttgatt tgagtcttga tggccacttg 2400 ctttttatgt ctcaatccta agagttgata cttacttagt attaatcagg tttcacatta 2460 tctcagacct agcttgcatt tttgatacaa acatgtcatg caatacacat aagcaacacg 2520 taccgagtca ttgattgaaa aacatgacct aaatagtaat acggactgag agagagtgat 2580 gaatcatttc atatgggaga cagctttctc atacgcgtcc atgcctttgc atgaaagatt 2640 aaataattca actctttcac ttatcttatt ttaatttact ttgcagaatt ccaagtttta 2700 ttgcaatcac caggggtatt aaatgggata gtgtaaatgc acctgcaaca catcaactaa 2760 ccacacactc ctccacctgt ttgtatttac ttcaaaaact taattcaaaa atccctttgc 2820 ttctttcctt ccctctattt ataaacccta gtatccggtt tttattctac cacacaatac 2880 catttgcaac aaacttctct ctgaatctca gtgaattctc ataaaccatg gaaactcaac 2940 aacaacaagc agatattcaa gattttggga acaaaaacag taacacctat gcatgcaggc 3000 aaagcagtac taggtggaca cccacaagtg atcagatcag aatactgaaa gagctctact 3060 acaacaatgg aatcagatca ccaactgctg atcagattca gagaatcgct gctcaactca 3120 gacagtacgg caagattgaa ggcaagaacg tgttttattg gtttcagaac cataaagctc 3180 gtgaacgcca gaagaaacgg ttcactccta ctgctcctcc accaccgtct cacttctccg 3240 atcatattaa ccaccatctt cccaacgctg cccccatgca gattccgtct caccaccacc 3300 actaccacca tcaagaacca ccacatgtct atgctcaccc tcacaaactc tacaccactc 3360 atcacattgg ggttggttct tcttcacaag gagtgatggg tgtagggtgt ggctatggat 3420 ctgttgctat ggagaagagt ttcaggaagt gttcaatatc accaccggga gagagtaagg 3480 ccacaggagg aattggccgg aatatcggtt caagatcacg gataagtgtc gattcgtgtt 3540 ccttcttcga cacaattaag ccaaagacat atgagatgtt tgagaatcat gatcaagatg 3600 aagaacaagg agagccttca actgagatcg aaacacttcc attattcccg attcacgacg 3660 gcaaccatca tgatttcttc ggcatgagga cggcaaatct gcaattggag caaggcactg 3720 gaggttacta cactggagga agctggtatc ggtctgatga gagggcgtct cttgagctca 3780 gtctcaactc atatggttat tataactaat tcgaactggc caggtacctg acatcttata 3840 gtctgcaacc tctcgtgtct gaattcctat ctttatcaag tgttattgct tccacgacta 3900 taggacagct ttcgtcgaaa ggttttgctc atgtgatctc gaaggattca tctagtctga 3960 tttttcgtga cttgtatcgg ttttattgga ttcatccaac atatatcaat aaaaaatgag 4020 ttgtgtttcc tttcttccta gttcagttaa aattatttcc ctcctgcgct tgtgctgtaa 4080 ttgtctgtgt acctgttgtt tgtgactgtg ttagttccct tggatatgat ttcgtatttg 4140 atatgtacat ggagatagct tagcttcatt attggagtat gaagttagta tgacatagtc 4200 actctcctgg aaaattgaca ctgcaaacca tatttttatt ctgaaccaca aatcctagtc 4260 agtccgctgg catatgccgt ccgtttgctg aatccagaac gtgggtttgg agatgtacgg 4320 ctgagatgcc tctatgcgaa ggggatttcg tggtgaaacg agatgggagt agagcaacgc 4380 ccgtggaaga tgcttcaaac ttccacactt ttgagcaacg atcggcagta gtaaggtaga 4440 cgatttcaag atcaaagcat atgaagataa acaacatcaa caacaaaatt tgttggggtt 4500 ctatagagag aaacagagct acatacatac actgttttgt atctaccatc tgagatgatg 4560 aaaagatgaa aaactaaaga atgccccggc gccaacgcca ggacacgccg cgcgcgcgtc 4620 acccgagcca tctcttgacc cagccggcgc tgtatattta cacacgttgc agcatcgatc 4680 accacctgtt cgatcgcgtc gccgtcacca agcttggcgc gccggtcacc cggtccgggc 4740 ctagaaggcc acccggggct cggcgttagg cttttagtcc gctaggtggc tagaccgagg 4800 ttaggacgct agttcactag gggcctagtg gtgttttaag gatgggcaag tttgtacaaa 4860 aaagcaggct ggcgccggaa ccaattcagt cgagtgcggc cgccagtgtg atggatatct 4920 cgacccgggt gattgcggtt acatcatgta cggaaaaata attctaatcc ttgatttaaa 4980 tttgaacttg actatttatt tattctttat ttcattttgt aaatcatttt atgtatctcc 5040 tggcaagcaa ttttatccac cttgcaccaa caccttcggg ttccataatc aaaccacctt 5100 aacttcacac catgctgtaa ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa 5160 tttaataaac aatcatacga agcagtgaca aaataccaga tggtattaat gcttcgataa 5220 aattaattgg aaagtataaa atggtagaaa ataataaatt ataattaatt taagtaagat 5280 aaaaaataat taaaaactaa aatgttaaaa ttttaaaaaa attattttaa ataatattta 5340 aaaacattaa aaatcatttt aaaaaattta tttatagaac aattaaataa atatttcagc 5400 taataaaaaa caaaagctta cctagcctta gaagacaact tgtccaacaa ttagatgata 5460 cccattgccc ttacgttttc tttaacatca attattgttt ttgtcaacaa gctatctttt 5520 agttttattt tattggtaaa aaatatgtcg ccttcaagtt gcatcattta acacatctcg 5580 tcattagaaa aataaaactc ttccctaaac gattagtaga aaaaatcatt cgataataaa 5640 taagaaagaa aaattagaaa aaaataactt cattttaaaa aaatcattaa ggctatattt 5700 tttaaatgac taattttata tagactgtaa ctaaaagtat acaatttatt atgctatgta 5760 tcttaaagaa ttacttataa aaatctacgg aagaatatct tacaaagtga aaaacaaatg 5820 agaaagaatt tagtgggatg attatgattt tatttgaaaa ttgaaaaaat aattattaaa 5880 gactttagtg gagtaagaaa gctttcctat tagtcttttc ttatccataa aaaaaaaaaa 5940 aaaaatctag cgtgacagct tttccataga ttttaataat gtaaaatact ggtagcagcc 6000 gaccgttcag gtaatggaca ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat 6060 aacgccgtgg taacggataa agccaagcgt gaagcggtga aggtacatct ctgactccgt 6120 caagattacg aaaccgtcaa ctacgaagga ctccccgaaa tatcatctgt gtcataaaca 6180 ccaagtcaca ccatacatgg gcacgcgtca caatatgatt ggagaacggt tccaccgcat 6240 atgctataaa atgcccccac acccctcgac cctaatcgca cttcaattgc aatcaaatta 6300 gttcattctc tttgcgcagt tccctacctc tcctttcaag gttcgtagat ttcttccgtt 6360 tttttttctt cttctttatt gtttgttcta catcagcatg atgttgattt gattgtgttt 6420 tctatcgttt catcgattat aaattttcat aatcagaaga ttcagctttt attaatgcaa 6480 gaacgtcctt aattgatgat tttataaccg taaattaggt ctaattagag tttttttcat 6540 aaagattttc agatccgttt acaacaagcc ttaattgttg attctgtagt cgtagattaa 6600 ggtttttttc atgaactact tcagatccgt taaacaacag ccttatttgt tgatacttca 6660 gtcgtttttc aagaaattgt tcagatccgt tgataaaagc cttattcgtt gattctgtat 6720 ggtatttcaa gagatattgc tcaggtcctt tagcaactac cttatttgtt gattctgtgg 6780 ccatagatta ggattttttt tcacgaaatt gcttcttgaa attacgtgat ggattttgat 6840 tctgatttat cttgtgattg ttgactctac agccatggcc cacagcaagc acggcctgaa 6900 ggaggagatg accatgaagt accacatgga gggctgcgtg aacggccaca agttcgtgat 6960 caccggcgag ggcatcggct accccttcaa gggcaagcag accatcaacc tgtgcgtgat 7020 cgagggcggc cccctgccct tcagcgagga catcctgagc gccggcttca agtacggcga 7080 ccggatcttc accgagtacc cccaggacat cgtggactac ttcaagaaca gctgccccgc 7140 cggctacacc tggggccgga gcttcctgtt cgaggacggc gccgtgtgca tctgtaacgt 7200 ggacatcacc gtgagcgtga aggagaactg catctaccac aagagcatct tcaacggcgt 7260 gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc accaactggg aggccagctg 7320 cgagaagatc atgcccgtgc ctaagcaggg catcctgaag ggcgacgtga gcatgtacct 7380 gctgctgaag gacggcggcc ggtaccggtg ccagttcgac accgtgtaca aggccaagag 7440 cgtgcccagc aagatgcccg agtggcactt catccagcac aagctgctgc gggaggaccg 7500 gagcgacgcc aagaaccaga agtggcagct gaccgagcac gccatcgcct tccccagcgc 7560 cctggcctga gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa 7620 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta 7680 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta 7740 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg 7800 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattctag tggccggccc 7860 agctgatatc tagacccagc tttcttgtac aaagtggccg ttaacggatc ggccagaatg 7920 gcccggaccg ggttaccgaa ttcgagctcg gtaccctggg atccgatatc gatgggccct 7980 ggccgaagct tggtcacccg gtccgggcct agaaggccag cttcggccgc cccgggcaac 8040 tttattatac aaagttgatg gtacggatcc gtcgacggcg cgccgcggac ctgaattcca 8100 gcacactggc ggccgttagt ctaaaaacgg cccctaacta acgcttagtt gcccgctaga 8160 ttagtccccc gttagtgcct agttagacac acgacgaact aggggctact agtaacggcc 8220 gccagtgtgc tcggtacggc cagaatggcc atctggattc agcagggggt gattgcggtt 8280 acatcatgta cggaaaaata attctaatcc ttgatttaaa tttgaacttg actatttatt 8340 tattctttat ttcattttgt aaatcatttt atgtatctcc tggcaagcaa ttttatccac 8400 cttgcaccaa caccttcggg ttccataatc aaaccacctt aacttcacac catgctgtaa 8460 ctcacaccgc ccagcatctc caatgtgaaa gaagctaaaa tttaataaac aatcatacga 8520 agcagtgaca aaataccaga tggtattaat gcttcgataa aattaattgg aaagtataaa 8580 atggtagaaa ataataaatt ataattaatt taagtaagat aaaaaataat taaaaactaa 8640 aatgttaaaa ttttaaaaaa attattttaa ataatattta aaaacattaa aaatcatttt 8700 aaaaaattta tttatagaac aattaaataa atatttcagc taataaaaaa caaaagctta 8760 cctagcctta gaagacaact tgtccaacaa ttagatgata cccattgccc ttacgttttc 8820 tttaacatca attattgttt ttgtcaacaa gctatctttt agttttattt tattggtaaa 8880 aaatatgtcg ccttcaagtt gcatcattta acacatctcg tcattagaaa aataaaactc 8940 ttccctaaac gattagtaga aaaaatcatt cgataataaa taagaaagaa aaattagaaa 9000 aaaataactt cattttaaaa aaatcattaa ggctatattt tttaaatgac taattttata 9060 tagactgtaa ctaaaagtat acaatttatt atgctatgta tcttaaagaa ttacttataa 9120 aaatctacgg aagaatatct tacaaagtga aaaacaaatg agaaagaatt tagtgggatg 9180 attatgattt tatttgaaaa ttgaaaaaat aattattaaa gactttagtg gagtaagaaa 9240 gctttcctat tagtcttttc ttatccataa aaaaaaaaaa aaaaatctag cgtgacagct 9300 tttccataga ttttaataat gtaaaatact ggtagcagcc gaccgttcag gtaatggaca 9360 ctgtggtcct aacttgcaac gggtgcgggc ccaatttaat aacgccgtgg taacggataa 9420 agccaagcgt gaagcggtga aggtacatct ctgactccgt caagattacg aaaccgtcaa 9480 ctacgaagga ctccccgaaa tatcatctgt gtcataaaca ccaagtcaca ccatacatgg 9540 gcacgcgtca caatatgatt ggagaacggt tccaccgcat atgctataaa atgcccccac 9600 acccctcgac cctaatcgca cttcaattgc aatcaaatta gttcattctc tttgcgcagt 9660 tccctacctc tcctttcaag gttcgtagat ttcttccgtt tttttttctt cttctttatt 9720 gtttgttcta catcagcatg atgttgattt gattgtgttt tctatcgttt catcgattat 9780 aaattttcat aatcagaaga ttcagctttt attaatgcaa gaacgtcctt aattgatgat 9840 tttataaccg taaattaggt ctaattagag tttttttcat aaagattttc agatccgttt 9900 acaacaagcc ttaattgttg attctgtagt cgtagattaa ggtttttttc atgaactact 9960 tcagatccgt taaacaacag ccttatttgt tgatacttca gtcgtttttc aagaaattgt 10020 tcagatccgt tgataaaagc cttattcgtt gattctgtat ggtatttcaa gagatattgc 10080 tcaggtcctt tagcaactac cttatttgtt gattctgtgg ccatagatta ggattttttt 10140 tcacgaaatt gcttcttgaa attacgtgat ggattttgat tctgatttat cttgtgattg 10200 ttgactctac agccatggct gcaactactc ttacatctgc tttacctggt gctttcagtt 10260 catcccagag accaagtgct cctttcaact tacaaagatc acctagagtg cttagaaggt 10320 tcaacagaaa gacaggacgt caacctcgtg gacttgttcg tgctgcaaag gctcaagcgg 10380 cgcaaaacct gccagagaaa ttcgatgaaa caagactctg ggaggccctc aggggatttg 10440 gggttagtcc aagtagggtt acttacgctc ccgtcggatt cggagattac cactggaccg 10500 tcacagatga agacgggaga ccttggttcg caactgtcag tgacctcgaa cacaaagagc 10560 attgcgggca gggagcacaa gcagccctta aaggactcag acaagctatg gacactgccc 10620 tcgcacttag agatagggac ggactcagat tcgtggttgc tccagtggca gcaacagatg 10680 ggggtggccc tgtccttccc cttgacgcta ggtatgcact cacagtcttc cctcacgtcc 10740 caggaagaac aggagagttc ggacaaagac tcacagaggc agagagggac agactgcttg 10800 ctcttctggc agaactccac ggaagaacac ctcctgaaac aaccccacca gccgatatgg 10860 aaccaccagg acttcctggt gttagagcag cactcgccga aagtgaagga ccttggagtg 10920 gaggaccatt tgcagaaccc gctagacttc ttctcagaga acacgaggcc acactgcacg 10980 caagactcgc tgaattcgaa gccctcgtcg caagagttaa gggtcgtgga gcacctcttg 11040 tcgttaccca cggagaacca cacccaggca acttgatcct cggagaggaa ggctatctcc 11100 tcgtggactg ggatacagtt ggactggctc cagctgaaag ggatttgagt ctcatctcag 11160 acgacccagc tgacctcgca agatacgcag agcttacagg aagaactcca gacccagacg 11220 cactcgcact ctatagactg cgttggagtc tccttgatgt ggctgagttc gtcgagtggt 11280 tcagggctga acaccaaaga acccaagaca ccgagagtgc atggaagggg ttcactgaca 11340 cacttgggca attggctgct ggtggatcag ctgcatagca aacttatctc tgttatgaat 11400 cagaagaagt tcatgtctcg tttcatttaa aactttggtg gtttgtgttt tggggccttg 11460 taaagcccct gatgaataat tgttcaacta tgtttccgtt cctgtgttat acctttcttt 11520 ctaatgagta atgacatcaa acttcttctg tattgaaatt atgtccttgt gagtctcttt 11580 atcatcgttt cgtctttaca ttatatgtgc tacttttgtc taatgagcct gaaaagtggc 11640 tccaatggta cgcactggaa gatttgttgg cttctggtag atatagcgac agtgttgagc 11700 ttgtaatatc atgtctctta ttgctaaatt agttcctttc ttaacagaaa ccttcaaagt 11760 ttttgttttt gttttcattt acctaatgta cacatacgct ggccatgact aacaacatgt 11820 ccaggcttag agcatatttt tttctagctt aaattgttaa cttgtcattc agtaaaatcc 11880 gagaattgtg aagctctaat tgaagctaat tcgttttata aagtcagtta aaaagtatac 11940 taaattatcc aacttttctt caaaatctca aaattctatg acaaaacgat agtctttgtt 12000 tatgtcagta ccacaaagag gtggaaaaaa acaccaaaaa aacaataagc aaactataca 12060 ctgagaagaa aaataaaaga gagctcaata gatgttttat actaacggta gattagatca 12120 aagatccaag ctttactcta catagagcag aacccagaat cccttcatat ctcttttatt 12180 ctagcaccga taatctactg aaaagaagac acttagagct ctgtctcttt gtcaaagaag 12240 tcccagccgt catccagaag ctccttacgt tcattaacag aggccgcaat tggatcgggt 12300 ttacttattt tgtgggtatc tatactttta ttagattttt aatcaggctc ctgatttctt 12360 tttatttcga ttgaattcct gaacttgtat tattcagtag atcgaataaa ttataaaaag 12420 ataaaatcat aaaataatat tttatcctat caatcatatt aaagcaatga atatgtaaaa 12480 ttaatcttat ctttatttta aaaaatcata taggtttagt atttttttaa aaataaagat 12540 aggattagtt ttactattca ctgcttatta cttttaaaaa aatcataaag gtttagtatt 12600 tttttaaaat aaatatagga atagttttac tattcactgc tttaatagaa aaatagttta 12660 aaatttaaga tagttttaat cccagcattt gccacgtttg aacgtgagcc gaaacgatgt 12720 cgttacatta tcttaaccta gctgaaacga tgtcgtcata atatcgccaa atgccaactg 12780 gactacgtcg aacccacaaa tcccacaaag cgcgtgaaat caaatcgctc aaaccacaaa 12840 aaagaacaac gcgtttgtta cacgctcaat cccacgcgag tagagcacag taaccttcaa 12900 ataagcgaat ggggcataat cagaaatccg aaataaacct aggggcatta tcggaaatga 12960 aaagtagctc actcaatata aaaatctagg aaccctagtt ttcgttatca ctctgtgctc 13020 cctcgctcta tttctcagtc tctgtgtttg cggctgagga ttccgaacga gtgaccttct 13080 tcgtttctcg caaaggtaac agcctctgct cttgtctctt cgattcgatc tatgcctgtc 13140 tcttatttac gatgatgttt cttcggttat gtttttttat ttatgcttta tgctgttgat 13200 gttcggttgt ttgtttcgct ttgtttttgt ggttcagttt tttaggattc ttttggtttt 13260 tgaatcgatt aatcggaaga gattttcgag ttatttggtg tgttggaggt gaatcttttt 13320 tttgaggtca tagatctgtt gtatttgtgt tataaacatg cgactttgta tgatttttta 13380 cgaggttatg atgttctggt tgttttatta tgaatctgtt gagacagaac catgattttt 13440 gttgatgttc gtttacacta ttaaaggttt gttttaacag gattaaaagt tttttaagca 13500 tgttgaagga gtcttgtaga tatgtaaccg tcgatagttt ttttgtgggt ttgttcacat 13560 gttatcaagc ttaatctttt actatgtatg cgaccatatc tggatccagc aaaggcgatt 13620 ttttaattcc ttgtgaaact tttgtaatat gaagttgaaa ttttgttatt ggtaaactat 13680 aaatgtgtga agttggagta tacctttacc ttcttatttg gctttgtgat agtttaattt 13740 atatgtattt tgagttctga cttgtatttc tttgaattga ttctagttta agtaatcatg 13800 gcctcctccg agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc 13860 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac 13920 aacaccgtga agctgaaggt gacgaagggc ggccccctgc ccttcgcctg ggacatcctg 13980 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac 14040 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 14100 ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag 14160 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg 14220 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc 14280 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac 14340 atggccaaga agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc 14400 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac 14460 cacctgttcc tgtagttttg tgatctgatg ataagtggtt ggttcgtgtc tcatgcactt 14520 gggaggtgat ctatttcacc tggtgtagtt tgtgtttccg tcagttggaa aaacttatcc 14580 ctatcgattt cgttttcatt ttctgctttt cttttatgta ccttcgtttg ggcttgtaac 14640 gggcctttgt atttcaactc tcaataataa tccaagtgca tgttaaacaa tttgtcatct 14700 gtttcggctt tgatatacta ctggtgaaga tgggccgtac tactgcatca caacgaaaaa 14760 taataataag atgaaaaact tgaagtggaa aaaaaaaaaa acttgaatgt tcactactac 14820 tcattgacca taatgtttaa catacatagc tcaatagtat ttttgtgaat atggcaacac 14880 aaacagtcca aaacaattgt ctcttactat accaaaccaa gggcgccgct tgtttgccac 14940 tctttgtgtg caatagtgtg attaccacat ctccacattc aatatattcc ctgaattatc 15000 tgacgatttt gatggctcac tgttttccca agtcttgaat tgtcttctgt gcgccagtca 15060 aatgcatatg tgttgagttt atcttttaaa tatcaagctt ttgtttttaa cttttgtttg 15120 taaccaaaaa ctcacagtag gagtttgatc acataatttt atgtttgcct ttgcaatttc 15180 tagtgagtct ttgattaaaa gcttgaaaag aaaatgcagc caagcttacc aagtaagtta 15240 tgtgtattaa ccagaggaag agagaatctt gcaaaatttc aacaaacaca aaaagaagta 15300 ttactacgat tggtggagaa agaaaacgat tccaaatctt gaactgttgt tgtaaaagca 15360 tagcagaaag tgggagacaa ccgaaataga aatgactata acttaattta atgttatcat 15420 tataatttct tctagcaaat atttagaaag taaatatcac atcaaccttt aatgtaatta 15480 agctttctct ttttgattca tgtgagatga aaagaaaaaa aagaagagaa aagtgtagaa 15540 aacacatcat ttctaagctg aagctgaggt acaaattaat taataagtga ctagggtcac 15600 gtgaccctag tcacttatga ttccgatgac ttcgtaggtt cctagctcaa gccgctcgtg 15660 tccaagcgtc acttacgatt agctaatgat tacggcatct aggaccgact agttcttatg 15720 tgcttctagt ctccaaatgt ggttgatagt tattttgctc taagatcaac agtaatgaag 15780 tataaatcat cgttgtggtg tgctactcgg ttaattgagc attaacacac acaaacatga 15840 cgaggatggt ataatctcca aaaatgtgta ctttgttagg tgggacccta tagccttgat 15900 taatgtgcta tgttaggcat gcctggaaac gtgtgacgca tatgttttgt gaacctgttg 15960 atattatatg tgcttttata ttaccatatt ttattaaaat actaatattt attactagta 16020 agatataaca ttctatctag cttaaaaact aaccataaat attccataat aactagattt 16080 accaaactaa tatactaaat atacataata aatacaaaat taacaagaca ataatcaata 16140 tttatgagct taatatattt agacattatg gttggtcgac gataatcatg ctaacttttc 16200 gtaattgctt gattgaaata tgcttagaat aatgcctctt tgttctacat ggcaaatagg 16260 gaccattatg gtgtaacacc ctgggaacca caaacacccc gaaatgctac taaactacac 16320 aactaacctt catatataaa atttcgacag catctccttt gaaaatttgc atagacgtgg 16380 aagcaacaga gtataaacag atctagctag ctaagtttaa acggccggcc aaacgtgcaa 16440 gcgctactag acaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag 16500 cgtcaattta tttacaattg aatatatcct gcca 16534 <210> 176 <211> 286 <212> DNA <213> Artificial Sequence <220> <223> PCR Target1 <400> 176 ccggaatcgg acctcgggac gtcgccaaag ttagctaggt gtccttagcg tttcttaggt 60 tctaggcgtt aggggtcaat aggttagcct agaagtcccg cactaacagc ctagcttaac 120 agttccaagc taagccacta gactagccct atccgactaa cctagtctaa gccgaacggg 180 gatctaatct aagctaggca cccattagac tagttcacta gcgcgaccgc actaaactag 240 gctaaaggtt aatagcaact agttagctag gggacgttag ctgccc 286 <210> 177 <211> 286 <212> DNA <213> Artificial Sequence <220> <223> PCR Target2 <400> 177 gacgtcccga ggtccgattc cgggctaatt aacacctagc tcgccactcg actaattagg 60 gactaagcct agcgcttagc cgttaaaacc tagcacaccc taagcaccct tagttaggtt 120 cccctcttaa ttaagcccta gtgagcccct aagttaaggg gacgctaaga gccccctaac 180 ctagtattcg gctagaggcg aactaggcta aacacctaag cgcacctctt taagctagat 240 cgctaggggg ctaggctaga gcttcgctag attagtctaa gggcag 286 <210> 178 <211> 100 <212> DNA <213> Artificial Sequence <220> <223> PCR Target3 <400> 178 gcgactaatt aactagctgt cccacggcct aactagcact taatccccta gcctaaccta 60 agagcgctaa tctaggctag tggtcactta gggctttaag 100 <210> 179 <211> 64 <212> DNA <213> Artificial Sequence <220> <223> PCR Target4 <400> 179 tcgggacgtc gctagtcact gattagagac ggtctgcact aactaacggc tagtctcctc 60 cgac 64 <210> 180 <211> 59 <212> DNA <213> Artificial Sequence <220> <223> PCR Target5 <400> 180 attccggcct agtgcttagc taacgggaaa cccgcttaac gattagttag tggacgggg 59 <210> 181 <211> 75 <212> DNA <213> Artificial Sequence <220> <223> PCR Target6 <400> 181 ccagtttagt taggccgact tattgaacta gtctagggcg ttaggaactc tctaaggggt 60 tagcgtgtta gaggc 75 <210> 182 <211> 73 <212> DNA <213> Artificial Sequence <220> <223> PCR Target7 <400> 182 gctagtagac gctgctagtg actaaggttt aggtatcagc cctagctaac cgttagtctt 60 cgctcctaag tgc 73 <210> 183 <211> 84 <212> DNA <213> Artificial Sequence <220> <223> PCR Target8 <400> 183 ttaacggcaa acgctaatta gtcactaggc ccaggaaaca ctaactaagc cctagcttat 60 cctagcgcaa cctagttagg tctt 84 <210> 184 <211> 90 <212> DNA <213> Artificial Sequence <220> <223> PCR Target9 <400> 184 gtctaaaaac ggcccctaac taacgcttag ttgcccgcta gattagtccc ccgttagtgc 60 ctagttagac acacgacgaa ctaggggcta 90 <210> 185 <211> 81 <212> DNA <213> Artificial Sequence <220> <223> PCR Target10 <400> 185 gcgacttatt gaactagtct agggcgcggc taacaaccta gcttagattg ccttgtctaa 60 acccttaggc taacgcgcca c 81 <210> 186 <211> 90 <212> DNA <213> Artificial Sequence <220> <223> PCR Target11 <400> 186 agattagtgg gccaagcgtt taatcagggt taagagtggc tactaacggc taggtcgcta 60 gctatttcct cgtgagacct tcttgtaact 90 <210> 187 <211> 83 <212> DNA <213> Artificial Sequence <220> <223> PCR Target12 <400> 187 gctcggcgtt aggcttttag tccgctaggt ggctagaccg aggttaggac gctagttcac 60 taggggccta gtggtgtttt aag 83 <210> 188 <211> 75 <212> DNA <213> Artificial Sequence <220> <223> PCR Target13 <400> 188 attaagaccc gtcgtcaagt aggaattcca gaaatcaacg cacggtaact aaatcctcgc 60 gctaagctgg gtcag 75 <210> 189 <211> 1204 <212> DNA <213> Arabidopsis thaliana <400> 189 atatcggttg ttgacgatta accatgcaaa aaagaaacat taattgcgaa tgtaaataac 60 aaaacatgta actcttgtag atatacatgt atcgacattt aaacccgaat atatatgtat 120 acctataatt tctctgattt tcacgctacc tgccacgtac atgggtgata ggtccaaact 180 cacaagtaaa agtttacgta cagtgaattc gtctttttgg gtataaacgt acatttaatt 240 tacacgtaag aaaggattac caattctttc atttatggta ccagacagag ttaaggcaaa 300 caagagaaac atatagagtt ttgatatgtt ttcttggata aatattaaat tgatgcaata 360 tttagggatg gacacaaggt aatatatgcc ttttaaggta tatgtgctat atgaatcgtt 420 tcgcatgggt actaaaatta tttgtcctta ctttatataa acaaattcca acaaaatcaa 480 gtttttgcta aaactagttt atttgcgggt tatttaatta cctatcatat tacttgtaat 540 atcattcgta tgttaacggg taaaccaaac caaaccggat attgaactat taaaaatctt 600 gtaaatttga cacaaactaa tgaatatcta aattatgtta ctgctatgat aacgaccatt 660 tttgtttttg agaaccataa tataaattac aggtacgtga caagtactaa gtatttatat 720 ccacctttag tcacagtacc aatattgcgc ctaccgggca acgtgaacgt gatcatcaaa 780 tcaaagtagt taccaaacgc tttgatctcg ataaaactaa aagctgacac gtcttgctgt 840 ttcttaattt atttctctta caacgacaat tttgagaaat atgaaatttt tatatcgaaa 900 gggaacagtc cttatcattt gctcccatca cttgcttttg tctagttaca actggaaatc 960 gaagagaagt attacaaaaa catttttctc gtcatttata aaaaaatgac aaaaaattaa 1020 atagagagca aagcaagagc gttgggtgac gttggtctct tcattaactc ctctcatcta 1080 ccccttcctc tgttcgcctt tatatccttc accttccctc tctcatcttc attaactcat 1140 cttcaaaaat accctaatca cattttgtaa caataataca attatacatt aaaactctcc 1200 gacg 1204

Claims (111)

  1. (a) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (b) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (c) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (d) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 단편 또는 변이체; 또는
    (e) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드
    로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 갖는 조직-선호적 프로모터를 포함하는 형태형성 유전자 카세트를 포함하는 핵산 분자로서, (a) 내지 (e)의 조직-선호적 프로모터는 형태형성 유전자에 작동 가능하게 연결되는, 핵산 분자.
  2. 제1항의 형태형성 유전자 카세트를 포함하는 발현 카세트.
  3. 제2항의 발현 카세트를 포함하는 벡터.
  4. 제2항의 발현 카세트를 포함하는 식물 세포.
  5. 제4항에 있어서, 식물 세포는 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물에서 유래된, 식물 세포.
  6. 제5항에 있어서, 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물은 옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 또는 배추속으로 이루어진 군으로부터 선택되는, 식물 세포.
  7. 제4항에 있어서, 형태형성 유전자는 WUS/WOX 호메오박스 폴리펩티드를 암호화하고,
    WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 식물 세포.
  8. 제4항에 있어서, 형태형성 유전자는 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생 개시, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 식물 세포.
  9. 제4항에 있어서, 상기 발현 카세트는 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종 폴리뉴클레오티드를 포함하는 형질 유전자 카세트를 추가로 포함하는, 식물 세포.
  10. 제9항에 있어서, 상기 발현 카세트는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 식물 세포.
  11. 제10항에 있어서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 식물 세포.
  12. 제11항에 있어서, 상기 발현 카세트의 형태형성 유전자 카세트 및 부위-특이적 재조합효소 카세트는 식물 세포에서 일시적으로 발현되고, 상기 발현 카세트의 형질 유전자 카세트는 식물 세포의 게놈에 안정적으로 통합되는, 식물 세포.
  13. 제11항에 있어서, 상기 발현 카세트의 형태형성 유전자 카세트 및 부위-특이적 재조합효소 카세트는 식물 세포로부터 절제되고, 상기 발현 카세트의 형질 유전자 카세트는 식물 세포의 게놈에 안정적으로 통합되는, 식물 세포.
  14. 제2항의 발현 카세트를 포함하는 식물.
  15. 제14항에 있어서, 식물은 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물인, 식물.
  16. 제15항에 있어서, 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물은 옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 또는 배추속으로 이루어진 군으로부터 선택되는, 식물.
  17. 제14항에 있어서, 형태형성 유전자는 WUS/WOX 호메오박스 폴리펩티드를 암호화하고,
    WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 식물.
  18. 제14항에 있어서, 형태형성 유전자는 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 식물.
  19. 제14항에 있어서, 상기 발현 카세트는 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종 폴리뉴클레오티드를 포함하는 형질 유전자 카세트를 추가로 포함하는, 식물.
  20. 제19항에 있어서, 상기 발현 카세트는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 식물.
  21. 제20항에 있어서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 식물.
  22. 제21항에 있어서, 상기 발현 카세트의 형태형성 유전자 카세트 및 부위-특이적 재조합효소 카세트는 식물에서 일시적으로 발현되고, 상기 발현 카세트의 형질 유전자 카세트는 식물의 게놈에 안정적으로 통합되는, 식물.
  23. 제22항의 식물의 종자로서, 상기 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  24. 제21항에 있어서, 상기 발현 카세트의 형태형성 유전자 카세트 및 부위-특이적 재조합효소 카세트는 식물로부터 절제되고, 상기 발현 카세트의 형질 유전자 카세트는 식물의 게놈에 안정적으로 통합되는, 식물.
  25. 제24항의 식물의 종자로서, 상기 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  26. 식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열은 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열의 100개 이상의 연속 뉴클레오티드를 가지며, 전사를 개시할 수 있는 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트.
  27. 식물 또는 식물 세포에서 전사를 개시할 수 있는 기능적 단편 또는 변이체를 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 기능적 단편 또는 변이체는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열로부터 유도되고, 전사를 개시할 수 있는 기능적 단편 또는 변이체는 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트.
  28. 식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열은 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열과 적어도 70%의 동일성을 가지며, 전사를 개시할 수 있는 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트.
  29. 식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열은 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되는 뉴클레오티드 서열과 적어도 95%의 동일성을 가지며, 전사를 개시할 수 있는 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트.
  30. 식물 또는 식물 세포에서 전사를 개시할 수 있는 뉴클레오티드 서열을 포함하는 재조합 폴리뉴클레오티드를 포함하는 발현 카세트로서, 뉴클레오티드 서열은 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나로 이루어진 군으로부터 선택되고, 전사를 개시할 수 있는 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결되는, 발현 카세트.
  31. 유전자이식 식물의 제조 방법으로서,
    재조합 발현 카세트로 식물 세포를 형질전환시키는 단계이되,
    (a) 조직-선호적 프로모터 카세트(상기 조직-선호적 프로모터 카세트는
    (i) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (ii) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (iii) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (iv) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열; 또는
    (v) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열
    로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 포함하고, (i) 내지 (v)의 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결됨) 및
    (b) 형질 유전자 카세트(상기 형질 유전자 카세트는 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종의 관심 폴리뉴클레오티드를 포함함)를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계;
    상기 재조합 발현 카세트를 갖는 유전자이식 식물 세포를 선별하는 단계; 및
    유전자이식 식물 세포로부터 유전자이식 식물을 재생시키는 단계를 포함하는, 방법.
  32. 제31항에 있어서, 식물 세포는 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물인, 방법.
  33. 제32항에 있어서, 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물은 옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 또는 배추속으로 이루어진 군으로부터 선택되는, 방법.
  34. 제31항에 있어서, 형태형성 유전자는 WUS/WOX 호메오박스 폴리펩티드를 암호화하고,
    WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 방법.
  35. 제31항에 있어서, 형태형성 유전자는 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 방법.
  36. 제31항에 있어서, 상기 발현 카세트는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법.
  37. 제36항에 있어서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법.
  38. 제37항에 있어서, 상기 재조합 발현 카세트로부터 상기 조직-선호적 프로모터 카세트 및 상기 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는, 방법.
  39. 제38항의 방법에 의해 제조된 유전자이식 식물.
  40. 제39항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  41. 제31항에 있어서, 상기 조직-선호적 프로모터 카세트는 제1 T-DNA를 포함하고 상기 형질 유전자 카세트는 제2 T-DNA를 포함하는, 방법.
  42. 제41항에 있어서, 제1 T-DNA 및 제2 T-DNA는 식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법.
  43. 제42항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  44. 제43항의 방법에 의해 제조된 유전자이식 식물.
  45. 제44항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  46. 제41항에 있어서, 제1 T-DNA는 제1 박테리아 균주에 존재하고 제2 T-DNA는 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주는 식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법.
  47. 제46항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  48. 제47항의 방법에 의해 제조된 유전자이식 식물.
  49. 제48항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  50. 체세포배 성숙 효율을 향상시키는 방법으로서,
    재조합 발현 카세트로 식물 세포를 형질전환시키는 단계이되,
    (a) 조직-선호적 프로모터 카세트(상기 조직-선호적 프로모터 카세트는
    (i) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (ii) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (iii) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (iv) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (v) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열
    로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 포함하고, (a) 내지 (e)의 뉴클레오티드 서열은 형태형성 유전자에 작동 가능하게 연결됨) 및
    (b) 형질 유전자 카세트(상기 형질 유전자 카세트는 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종의 관심 폴리뉴클레오티드를 포함함)를 포함하는 재조합 발현 카세트로 식물 세포를 형질전환시키는 단계;
    상기 재조합 발현 카세트를 갖는 유전자이식 식물 세포를 선별하는 단계; 및
    유전자이식 식물 세포로부터 유전자이식 식물을 재생시키는 단계를 포함하고, 상기 재조합 발현 카세트로 인해 상기 재조합 발현 카세트를 포함하지 않는 유전자이식 식물 세포에 비해 체세포배 성숙 효율이 향상되는, 방법.
  51. 제50항에 있어서, 식물 세포는 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물인, 방법.
  52. 제51항에 있어서, 외떡잎식물, 쌍떡잎식물, 또는 겉씨식물은 옥수수, 알팔파, 수수, 벼, 밀렛, 대두, 밀, 목화, 해바라기, 보리, 귀리, 호밀, 아마, 사탕수수, 바나나, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 대나무, 라이밀, 멜론, 또는 배추속으로 이루어진 군으로부터 선택되는, 방법.
  53. 제50항에 있어서, 형태형성 유전자는 WUS/WOX 호메오박스 폴리펩티드를 암호화하고,
    WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 방법.
  54. 제50항에 있어서, 형태형성 유전자는 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 방법.
  55. 제50항에 있어서, 상기 발현 카세트는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법.
  56. 제55항에 있어서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법.
  57. 제56항에 있어서, 상기 재조합 발현 카세트로부터 상기 조직-선호적 프로모터 카세트 및 상기 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는, 방법.
  58. 제57항의 방법에 의해 제조된 유전자이식 식물.
  59. 제58항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  60. 제50항에 있어서, 상기 조직-선호적 프로모터 카세트는 제1 T-DNA를 포함하고 상기 형질 유전자 카세트는 제2 T-DNA를 포함하는, 방법.
  61. 제60항에 있어서, 제1 T-DNA 및 제2 T-DNA는 식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법.
  62. 제61항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  63. 제62항의 방법에 의해 제조된 유전자이식 식물.
  64. 제63항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  65. 제60항에 있어서, 제1 T-DNA는 제1 박테리아 균주에 존재하고 제2 T-DNA는 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주는 식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법.
  66. 제65항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  67. 제66항의 방법에 의해 제조된 유전자이식 식물.
  68. 제67항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  69. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서,
    재조합 발현 카세트로 쌍떡잎식물 세포 또는 겉씨식물 세포를 형질전환시키는 단계이되,
    (a) 조직-선호적 프로모터 카세트(상기 조직-선호적 프로모터 카세트는
    (i) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (ii) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (iii) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일하고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열;
    (iv) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나이고 식물 세포에서 전사를 개시하는 단편 또는 변이체;
    (v) 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편이고 식물 세포에서 전사를 개시하는 뉴클레오티드 서열의 100 bp 이상의 단편
    으로 이루어진 군으로부터 선택되는 뉴클레오티드 서열을 포함하고, 식물 세포에서 전사를 개시하는 (i) 내지 (v)의 뉴클레오티드 서열은 WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열에 작동 가능하게 연결됨) 및
    (b) 형질 유전자 카세트(상기 형질 유전자 카세트는 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는 이종의 관심 폴리뉴클레오티드를 포함함)를 포함하는 재조합 발현 카세트로 쌍떡잎식물 세포 또는 겉씨식물 세포를 형질전환시키는 단계;
    각각의 형질전환된 식물 세포에서 상기 재조합 발현 카세트를 발현시켜 체세포배 또는 싹을 형성하는 단계; 및
    체세포배를 발아시키거나 싹을 배양하여, 이종의 관심 폴리뉴클레오티드를 포함하는 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물을 형성하는 단계를 포함하는, 방법.
  70. 제69항에 있어서, 체세포배 또는 싹은 쌍떡잎식물 세포 또는 겉씨식물 세포의 형질전환 개시 후 약 21일 내지 약 28일 내에 형성되는, 방법.
  71. 제69항에 있어서, 겉씨식물 세포는 소나무 및 미송으로 이루어진 군으로부터 선택되는, 방법.
  72. 제69항에 있어서, 쌍떡잎식물 세포는 알팔파, 대두, 목화, 해바라기, 아마, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 멜론, 또는 배추속으로 이루어진 군으로부터 선택되는, 방법.
  73. 제69항에 있어서, WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 방법.
  74. 제69항에 있어서, WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열은 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는 유전자 산물을 암호화하는, 방법.
  75. 제69항에 있어서, 상기 발현 카세트는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법.
  76. 제75항에 있어서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법.
  77. 제76항에 있어서, 상기 재조합 발현 카세트로부터 상기 조직-선호적 프로모터 카세트 및 상기 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는, 방법.
  78. 제77항의 방법에 의해 제조된 유전자이식 식물.
  79. 제78항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  80. 제69항에 있어서, 상기 조직-선호적 프로모터 카세트는 제1 T-DNA를 포함하고 상기 형질 유전자 카세트는 제2 T-DNA를 포함하는, 방법.
  81. 제80항에 있어서, 제1 T-DNA 및 제2 T-DNA는 식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법.
  82. 제81항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  83. 제82항의 방법에 의해 제조된 유전자이식 식물.
  84. 제83항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  85. 제80항에 있어서, 제1 T-DNA는 제1 박테리아 균주에 존재하고 제2 T-DNA는 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주는 식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법.
  86. 제85항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  87. 제86항의 방법에 의해 제조된 유전자이식 식물.
  88. 제86항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  89. 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물의 제조 방법으로서,
    (a) 이종의 관심 유전자를 포함하는 형질 유전자 카세트 및 WUS/WOX 호메오박스 폴리펩티드를 암호화하는 뉴클레오티드 서열을 포함하는 형태형성 유전자 카세트를 포함하는 재조합 발현 카세트로 쌍떡잎식물 외식편 또는 겉씨식물 외식편의 세포를 형질전환시키는 단계;
    (b) 각각의 형질전환된 세포에서 (a)의 재조합 발현 카세트를 발현시켜 체세포배 또는 싹을 형성하는 단계; 및
    (c) 체세포배를 발아시키거나 싹을 배양하여 유전자이식 쌍떡잎식물 또는 유전자이식 겉씨식물을 형성하는 단계를 포함하는 방법.
  90. 제89항에 있어서, 체세포배 또는 싹은 세포의 형질전환 개시 후 약 21일 내지 약 28일 내에 형성되는, 방법.
  91. 제89항에 있어서, 쌍떡잎식물 또는 겉씨식물은 알팔파, 대두, 목화, 해바라기, 아마, 카사바, 강낭콩, 동부, 토마토, 감자, 비트, 포도, 유칼립투스, 포플러, 소나무, 미송, 감귤류, 파파야, 카카오, 오이, 사과, 고추류, 멜론, 또는 배추속으로 이루어진 군으로부터 선택되는, 방법.
  92. 제89항에 있어서, WUS/WOX 호메오박스 폴리펩티드는 서열 번호 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 또는 148 중 어느 하나의 아미노산 서열을 포함하거나, 서열 번호 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 또는 147 중 어느 하나의 뉴클레오티드 서열에 의해 암호화되는, 방법.
  93. 제89항에 있어서, WUS/WOX 호메오박스 폴리펩티드는 식물 대사, 기관 발육, 줄기 세포 발육, 세포 성장 자극, 기관형성, 재생, 체세포 배발생, 체세포배 성숙 촉진, 정단 분열조직의 발생 및/또는 발육, 싹 분열조직의 발생 및/또는 발육, 싹의 발생 및/또는 발육, 또는 이들의 조합에 관여하는, 방법.
  94. 제89항에 있어서, WUS/WOX 호메오박스 폴리펩티드는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터로 이루어진 군으로부터 선택되는 프로모터에 작동 가능하게 연결되는, 방법.
  95. 제94항에 있어서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, LEA-D34, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 95% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나와 적어도 70% 동일한 뉴클레오티드 서열, 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 단편 또는 변이체, 또는 서열 번호 1~59, 108~110, 124~126, 149~152, 및 189 중 적어도 하나의 100 bp 이상의 단편으로 이루어진 군으로부터 선택되는, 방법.
  96. 제89항에 있어서, 이종의 관심 유전자는 영양분 강화, 수확량 증가, 무생물 스트레스 내성, 가뭄 내성, 내한성, 제초제 내성, 해충 저항성, 병원체 저항성, 곤충 저항성, 질소 이용 효율(NUE), 병해 저항성, 또는 대사 경로 변경 능력을 부여하는 유전자 산물을 암호화하는, 방법.
  97. 제89항에 있어서, 상기 재조합 발현 카세트는 FLP, FLPe, KD, Cre, SSV1, 람다 Int, 파이 C31 Int, HK022, R, B2, B3, Gin, Tn1721, CinH, ParA, Tn5053, Bxb1, TP907-1, 또는 U153으로 이루어진 군으로부터 선택되는 부위-특이적 재조합효소를 암호화하는 뉴클레오티드 서열을 포함하는 부위-특이적 재조합효소 카세트를 추가로 포함하고, 부위-특이적 재조합효소는 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터에 작동 가능하게 연결되는, 방법.
  98. 제97항에 있어서, 항시성 프로모터, 유도성 프로모터, 조직-특이적 프로모터, 또는 발육 조절 프로모터는 UBI, LLDAV, EVCV, DMMV, BSV(AY) PRO, CYMV PRO FL, UBIZM PRO, SI-UB3 PRO, SB-UBI PRO(ALT1), USB1ZM PRO, ZM-GOS2 PRO, ZM-H1B PRO(1.2 KB), IN2-2, NOS, 35S의 135 버전, ZM-ADF PRO(ALT2), AXIG1, DR5, XVE, GLB1, OLE, LTP2, HSP17.7, HSP26, HSP18A, AT-HSP811, AT-HSP811L, GM-HSP173B, 테트라사이클린, 에타메트설푸론, 또는 클로르설푸론에 의해 활성화된 프로모터, PLTP, PLTP1, PLTP2, PLTP3, SDR, LGL, LEA-14A, 또는 LEA-D34로 이루어진 군으로부터 선택되는, 방법.
  99. 제98항에 있어서, 상기 재조합 발현 카세트로부터 상기 형태형성 유전자 카세트 및 상기 부위-특이적 재조합효소 카세트를 절제하는 단계를 추가로 포함하는, 방법.
  100. 제99항의 방법에 의해 제조된 유전자이식 식물.
  101. 제100항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  102. 제89항에 있어서, 상기 형태형성 유전자 카세트는 제1 T-DNA를 포함하고 상기 형질 유전자 카세트는 제2 T-DNA를 포함하는, 방법.
  103. 제102항에 있어서, 제1 T-DNA 및 제2 T-DNA는 쌍떡잎식물 세포를 형질전환시키기 위한 동일한 박테리아 균주에 존재하는, 방법.
  104. 제103항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  105. 제104항의 방법에 의해 제조된 유전자이식 식물.
  106. 제105항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  107. 제102항에 있어서, 제1 T-DNA는 제1 박테리아 균주에 존재하고 제2 T-DNA는 제2 박테리아 균주에 존재하며, 제1 박테리아 균주와 제2 박테리아 균주는 식물 세포를 형질전환시키기 위한 비율로 혼합되는, 방법.
  108. 제107항에 있어서, 제2 T-DNA로부터 제1 T-DNA를 분리시키는 단계를 추가로 포함하는 방법.
  109. 제108항의 방법에 의해 제조된 유전자이식 식물.
  110. 제109항의 유전자이식 식물의 종자로서, 상기 재조합 발현 카세트의 형질 유전자 카세트를 포함하는 종자.
  111. 제89항에 있어서, 발아시키는 단계는 체세포배 또는 싹을 성숙 배지 또는 발아 배지로 옮겨 유전자이식 식물을 형성하는 단계를 포함하는, 방법.
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