KR20200074987A - 아데노바이러스 및 이의 용도 - Google Patents

아데노바이러스 및 이의 용도 Download PDF

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KR20200074987A
KR20200074987A KR1020207014975A KR20207014975A KR20200074987A KR 20200074987 A KR20200074987 A KR 20200074987A KR 1020207014975 A KR1020207014975 A KR 1020207014975A KR 20207014975 A KR20207014975 A KR 20207014975A KR 20200074987 A KR20200074987 A KR 20200074987A
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타코 길스 유일
수미트라 로이
셀리나 칸
제롬 에이치. 에이치. 브이. 커스터스
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Abstract

본 명세서에서 아데노바이러스 핵산 서열 및 상기 핵산 서열을 포함하는 아데노바이러스 벡터가 제공된다. 제공된 아데노바이러스 벡터는 대상체에서의 보호 면역 반응을 유도하는 데 사용될 수 있다.

Description

아데노바이러스 및 이의 용도
본 발명은 생명 공학에 관한 것이며, 더 구체적으로는, 아데노바이러스 벡터, 예컨대 숙주에서 항원을 전달하고 면역 반응을 유발하기 위한 복제 결함 아데노바이러스 벡터 분야 및 용도에 관한 것이다.
재조합 아데노바이러스 벡터는 유전자 요법 적용분야 및 백신에 대해 널리 적용된다. AdV-5 벡터-기반 백신은 다양한 동물 모델에서 강하고 보호적인 면역 반응을 유발하는 것으로 나타났다(예를 들어, WO2001/02607; WO2002/22080; 문헌[Shiver et al., Nature 415:331 (2002)]; [Letvin et al., Ann. Rev. Immunol. 20:73(2002)]; [Shiver and Emini, Ann. Rev. Med. 55:355(2004)] 참조). 그러나, 재조합 AdV-5 벡터-기반 백신의 효용은 인간 집단에서 AdV-5-특이적 중화 항체(NAb)의 높은 혈청유병률에 의해 제한될 가능성이 있을 것이다. 항-AdV-5 면역의 존재는 마우스, 레서스 원숭이 및 인간의 연구에서 AdV-5-기반 백신의 면역원성을 실질적으로 억제하는 것으로 나타났다.
가장 통상적인 인간 아데노바이러스, 예를 들어, AdV-5로 이전에 감염되었거나 치료된 개체에 이미 존재하는 면역의 존재를 피하기 위한 한 가지 유망한 전략은 이러한 이전에 존재하는 면역과 접하지 않는 아데노바이러스 혈청형으로부터의 재조합 벡터의 개발을 수반한다. 한 가지 이러한 전략은 비-인간 유인원 아데노바이러스의 사용에 기반하는데, 그 이유는 이들이 전형적으로 인간을 감염시키지 않고 인간 샘플에서 낮은 혈청유병률을 나타내기 때문이다. 비인간 유인원 아데노바이러스는 이들 바이러스가 시험관내 인간 세포를 감염시킬 수 있는 것으로 나타났기 때문에 인간 용도로 적용 가능하다(WO2003/000283; WO2004/037189).
따라서, 다량으로 생산 가능하고, 숙주에서 이미 존재하는 면역과 만나지 않지만, 여전히 면역원성이고 벡터에 삽입된 이종성 핵산에 의해 암호화되는 항원에 대해 강한 면역 반응을 유도할 수 있는 대안의 아데노바이러스 벡터 분야에 대한 필요가 있다.
헥손(hexon) 폴리펩티드를 암호화하는 단리된 핵산 서열이 제공된다. 특정 실시형태에서, 헥손 폴리펩티드 또는 이의 기능성 유도체는 서열번호 1의 아미노산 서열 또는 서열번호 1의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함한다.
특정 실시형태에서, 헥손 폴리펩티드는 서열번호 1의 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함한다. 특정 실시형태에서, 헥손 폴리펩티드 또는 이의 기능성 유도체는 BLY6 헥손 폴리펩티드의 아미노산 서열(서열번호 2) 또는 서열번호 2의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다.
또한 섬유 폴리펩티드 또는 이의 기능성 유도체를 암호화하는 단리된 핵산 서열이 제공된다. 특정 실시형태에서, 섬유 폴리펩티드는 서열번호 10의 아미노산 서열을 포함하는 섬유 노브(knob) 폴리펩티드 서열; 서열번호 11의 아미노산 서열을 포함하는 섬유 샤프트(shaft) 폴리펩티드 서열; 및 서열번호 12의 아미노산 서열을 포함하는 섬유 꼬리 폴리펩티드 서열 중 적어도 하나를 포함한다. 특정 실시형태에서, 섬유 폴리펩티드 또는 이의 기능성 유도체는 BLY6 섬유 폴리펩티드의 아미노산 서열(서열번호 3) 또는 서열번호 3의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다.
본 발명의 실시형태는 또한 본 발명의 섬유 및 헥손 핵산 서열에 의해 암호화된 단리된 섬유 및 헥손 폴리펩티드를 포함한다.
추가로, 본 명세서에 개시된 헥손 폴리펩티드 중 적어도 하나를 암호화하는 헥손 핵산 서열 및 본 명세서에 개시된 섬유 폴리펩티드 중 적어도 하나를 암호화하는 섬유 핵산 서열을 포함하는, 단리된 핵산이 본 명세서에 제공된다. 특정 실시형태에서, 본 명세서에 기재된 단리된 핵산을 포함하는 벡터가 본 명세서에 제공된다. 일 실시형태에서, 벡터는 바이러스 벡터이다. 다른 실시형태에서, 벡터는 발현 벡터이다. 바람직한 일 실시형태에서, 벡터는 아데노바이러스 벡터이다. 더 바람직하게는, 벡터는 이식유전자를 추가로 포함한다.
또한 본 명세서에 기재된 벡터를 포함하는 재조합 세포가 제공된다. 이러한 세포는 재조합 단백질 생산, 재조합 단백질 발현, 또는 벡터 또는 바이러스 입자의 생산에 사용될 수 있다. 또한 벡터를 생산하는 방법이 제공된다. 상기 방법은 (a) 벡터의 생산을 위한 조건 하에 본 명세서에 개시된 재조합 세포를 성장시키는 단계; 및 (b) 재조합 세포로부터 벡터를 단리시키는 단계를 포함한다.
특정 실시형태에서, 본 명세서에 개시된 벡터를 포함하는 면역원성 조성물이 제공된다. 또한 본 명세서에 개시된 면역원성 조성물을 대상체에게 투여하는 단계를 포함하는, 면역 반응의 유도가 필요한 대상체에서 면역 반응을 유도하는 방법이 제공된다.
특정 실시형태에서, (a) 적어도 하나의 이식유전자; 및 (b) 본 발명의 실시형태에 따른 헥손 폴리펩티드를 암호화하는 핵산 서열을 포함하는, 아데노바이러스 벡터가 제공된다. 헥손 폴리펩티드는, 예를 들어, 서열번호 1의 아미노산 서열 또는 서열번호 1의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함할 수 있다. 헥손 폴리펩티드는, 예를 들어, 서열번호 1의 아미노산 서열을 갖는 아미노산 서열을 포함할 수 있다. 특정 실시형태에서, 헥손 폴리펩티드는 BLY6 헥손 폴리펩티드의 아미노산 서열(서열번호 2) 또는 서열번호 2의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다.
특정 실시형태에서, (a) 적어도 하나의 이식유전자; 및 (b) 본 발명의 실시형태에 따른 섬유 폴리펩티드를 암호화하는 핵산 서열을 포함하는 아데노바이러스 벡터가 제공된다. 특정 실시형태에서, 섬유 폴리펩티드는 서열번호 10의 아미노산 서열을 포함하는 섬유 노브 폴리펩티드 서열; 서열번호 11의 아미노산 서열을 포함하는 섬유 샤프트 폴리펩티드 서열; 및 서열번호 12의 아미노산 서열을 포함하는 섬유 꼬리 폴리펩티드 서열 중 적어도 하나를 포함할 수 있다. 특정 실시형태에서, 섬유 폴리펩티드 또는 이의 기능성 유도체는 BLY6 섬유 폴리펩티드의 아미노산 서열(서열번호 3) 또는 서열번호 3의 아미노산에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다. 본 발명의 실시형태는 또한 (a) 적어도 하나의 이식유전자; (b) 본 발명의 실시형태에 따른 헥손 폴리펩티드를 암호화하는 핵산 서열; 및 (c) 본 발명의 실시형태에 따른 섬유 폴리펩티드를 암호화하는 핵산 서열을 포함하는 아데노바이러스 벡터를 포함한다.
특정 실시형태에서, 본 명세서에 제공된 아데노바이러스 벡터는 복제-결함 아데노바이러스 벡터(replication-defective adenovirus vector: rAd)이다. 일 실시형태에서, 아데노바이러스 벡터는 E1 결실을 포함할 수 있다. 특정 실시형태에서, 본 명세서에 제공된 아데노바이러스 벡터는 E3 결실을 추가로 포함할 수 있다. 아데노바이러스 벡터는 1종 이상의 유인원 아데노바이러스(SAdV), 예컨대 침팬지 아데노바이러스(예를 들어, ChAd3); 고릴라 아데노바이러스; 또는 레서스 아데노바이러스(예를 들어, rhAd51, rhAd52 또는 rhAd53)로부터의 아데노바이러스 핵산 서열을 포함하는 유인원 아데노바이러스 벡터일 수 있다. 아데노바이러스 벡터는 1종 이상의 인간 아데노바이러스(예를 들어, hAdV-4, hAdV-5, hAdV-26, hAdV-35)로부터의 아데노바이러스 서열을 포함하는 인간 아데노바이러스 벡터일 수 있다. 바람직하게는, 아데노바이러스 벡터는 1종 이상의 인간 아데노바이러스 핵산 서열을 포함하는 키메라 아데노바이러스 벡터이다. 인간 아데노바이러스 핵산 서열은, 예를 들어, 인간 아데노바이러스-4(hAdV-4), 인간 아데노바이러스-5(hAdV-5), 인간 아데노바이러스-26(hAdV-26) 또는 인간 아데노바이러스-35(hAdV-35)일 수 있다. 아데노바이러스 벡터는, 예를 들어, 인간 아데노바이러스-5(hAdV-5) E4 orf6 및 orf 6/7을 포함할 수 있다.
특정 실시형태에서, 이식유전자는 반전 말단 반복부(inverted terminal repeat: ITR)에 인접한다. 특정 실시형태에서, 이식유전자는 E1 결실에서 또는 이에 인접하여, E3 결실에서 또는 이에 인접하여, 그리고/또는 ITR에서 또는 이에 인접하여, 예를 들어, E4 영역과 우측 ITR(RITR) 사이에 위치된다.
특정 실시형태에서, 본 명세서에 제공된 아데노바이러스 벡터는 서열번호 13 또는 서열번호 14의 핵산 서열을 포함한다.
또한 본 명세서에 기재된 아데노바이러스 벡터 및 약제학적으로 허용 가능한 담체를 포함하는 면역원성 조성물 또는 백신이 제공된다. 면역 반응의 유도가 필요한 대상체에서 면역 반응을 유도하는 방법이 추가로 제공된다. 상기 방법은 본 명세서에 개시된 백신을 대상체에게 투여하는 단계를 포함한다. 백신의 생산 방법이 추가로 제공된다. 상기 방법은 본 명세서에 개시된 아데노바이러스 벡터를 약제학적으로 허용 가능한 담체와 조합하는 단계를 포함한다.
앞서 언급한 발명의 내용과, 다음의 본 출원의 바람직한 실시형태의 상세한 설명은 첨부된 도면과 함께 읽을 때 더 잘 이해될 것이다. 그러나, 본 출원은 도면에 나타낸 정확한 실시형태로 제한되지 않음이 이해되어야 한다.
도 1은 BLY6.FLuc 및 Ad49.FLuc에 의해 유도되는 세포성 및 체액성 면역 반응을 나타낸다. 도 1A는 실험 구성을 나타낸다. 도 1B는 인터페론 감마(IFN-γ) ELISPOT 분석에 의해 결정한 바와 같은 벡터-암호화된 항원(즉, Fluc, 반딧불이 루시퍼라제)에 대해 Ad26.FLuc, BLY6.FLuc 및 Ad49.FLuc에 의해 유도된 세포성 면역 반응을 나타낸다. y-축은 106개의 비장세포당 스팟 형성 단위(Spot Forming Unit: SFU)의 수를 나타내고, 점선은 배지 자극의 95% 백분위를 나타낸다.
도 2는 BLY6.RSVF-2A-GLuc에 의해 유도되는 세포성 및 체액성 면역 반응을 나타낸다. 도 2a는 실험 구성을 나타낸다. 도 2b는 3가지 상이한 농도(108, 109 및 1010개의 vp)에서 Ad26.RSVF-2A-GLuc로 또는 BLY6.RSVF-2A-GLuc로, 또는 1010개의 vp에서 Ad26.FLuc 또는 BLY6.FLuc로 면역화 후 8주에 수행한 바이러스 중화 분석(virus neutralization assay: VNA) 결과의 그래프를 나타낸다. 도 2c는 IFN-γ ELISPOT 분석에 의해 결정한 바와 같은 면역화 후 Ad26.RSVF-2A-GLuc 및 BLY6.RSVF-2A-GLuc에 의해 유도되는 세포성 면역 반응을 나타낸다. 도 2d는 면역화 후 8주에 면역화된 마우스의 혈청에서 Ad26.RSVF-2A-GLuc 또는 BLY6.RSVF-2A-GLuc에 의해 유도되는 RSVF-특이적 IgG 결합 항체의 그래프를 나타낸다.
도 3은 아데노바이러스 벡터 Ad35, Ad26, Ad49, Ad5, Ad4 및 BLY6으로 면역화된 마우스에서 유도된 상동성 및 이종성 아데노바이러스 중화 역가를 나타낸다.
도 4는 미국(US) 및 유럽 연합(EU)에 살고 있는 18세 내지 55세의 성인으로부터의 200개의 인간 코호트 혈청 샘플에서의 Ad35, Ad26, Ad5 및 BLY6의 혈청유병률을 나타낸다. 각각의 벡터에 대해 이들 혈청에서 측정한 중화 역가를 4개의 범주로 나누고(16 미만(음성), 16 내지 300, 300 내지 1,000, 1000 내지 4000 및 4000 초과), 표시한 바와 같이 차트에 나타내었다.
도 5는 플라스미드 pBLY6.dE1.dE3(서열번호 8)의 개략도를 나타낸다.
도 6은 플라스미드 pBLY6.dE1.dE3.5IXp(서열번호 9)의 개략도를 나타낸다.
도 7은 BLY6 헥손 폴리펩티드(서열번호 2) 및 SAdV-30-1 헥손 폴리펩티드(서열번호 4) 서열의 정렬을 나타낸다.
도 8은 생성 세포주 sPER.C6에서 신규한 벡터 BLY6.Fluc의 생산성을 나타낸다.
본 개시내용은 아데노바이러스 종 E에 할당된 신규한 고릴라 아데노바이러스 단리물의 단리 및 식별뿐만 아니라 상기 고릴라 아데노바이러스의 헥손 및 섬유 폴리펩티드의 가변 영역을 암호화하는 핵산을 포함하는 백신 벡터의 작제 및 평가에 적어도 부분적으로 기반한다. 아데노바이러스 벡터는 면역반응을 유발할 수 있고, 더 나아가, 인간에서 낮은 혈청유병률을 가진다. 아데노바이러스 벡터는 백신에 대해 제형화될 수 있고, 관심 대상의 특정 항원에 대해 보호 면역을 유도하는 데 사용될 수 있다.
다양한 간행물, 논문 및 특허가 배경기술에, 그리고 명세서 전체에 걸쳐 인용되거나 기재되며; 이들 참고문헌 각각은 그 전문이 본 명세서에 참고로 포함된다. 본 명세서에 포함된 문헌, 작용, 물질, 장치, 규약 등의 논의는 본 발명의 맥락을 제공하는 목적을 위한 것이다. 이러한 논의는 개시되거나 청구되는 임의의 발명에 대해 임의의 또는 모든 이들 대상이 선행 기술의 일부를 형성한다고 용인하는 것이 아니다.
달리 정의되지 않는 한, 본 명세서에서 사용되는 모든 기술 및 과학 용어는 본 발명과 관련된 분야의 당업자가 통상적으로 이해하는 것과 동일한 의미를 갖는다. 그 외에는, 본 명세서에서 사용되는 특정 용어는 명세서에 제시되는 의미를 갖는다.
본 명세서에서 그리고 첨부된 청구범위에서 사용되는 단수 형태는 문맥상 명확히 달리 지시되지 않는 한 복수의 대상을 포함한다는 것이 주목되어야 한다.
달리 언급되지 않는 한, 본 명세서에 기재되는 농도 또는 농도 범위와 같은 임의의 수치는 모든 경우에 "약"이라는 용어로 수식되는 것으로 이해될 것이다. 따라서, 수치는 전형적으로 열거된 값의 ±10%를 포함한다. 예를 들어, 1 ㎎/㎖의 농도는 0.9 ㎎/㎖ 내지 1.1 ㎎/㎖를 포함한다. 마찬가지로, 1% 내지 10%(w/v)의 농도 범위는 0.9%(w/v) 내지 11%(w/v)를 포함한다. 본 명세서에서 사용되는 바와 같은 수치 범위의 사용은 문맥상 명백히 달리 지시되지 않는 한, 이러한 범위 내의 정수 및 소수값을 포함하여, 그 범위 내의 모든 가능한 하위범위, 모든 개개 수치를 명백히 포함한다.
달리 나타내지 않으면, 구성요소의 시리즈에 선행하는 "적어도"라는 용어는 그 시리즈의 모든 구성요소를 지칭하는 것으로 이해될 것이다. 당업자는 단지 일상적인 실험을 이용하여 본 명세서에 기재된 발명의 구체적 실시형태에 대한 많은 등가물을 인식하거나 확인할 수 있을 것이다. 이러한 등가물은 본 발명에 의해 포함되는 것으로 의도된다.
본 명세서에서 사용되는 바와 같이, 용어 "포함하다(comprises)", "포함하는(comprising)", "포함하다(includes)", "포함하는(including)", "갖다", "갖는", "함유하다" 또는 "함유하는", 또는 이의 임의의 다른 변형은 언급된 정수 또는 정수들의 그룹을 포함하지만, 임의의 다른 정수 또는 정수들의 그룹을 배제하는 것을 의미하지는 않으며, 비-배타적이거나 개방형인 것으로 의도됨이 이해될 것이다. 예를 들어, 구성요소의 목록을 포함하는 조성물, 혼합물, 공정, 방법, 물품, 또는 장치는 반드시 그 구성요소에만 한정될 필요는 없으며, 명백히 열거되지 않거나 그러한 조성물, 혼합물, 공정, 방법, 물품 또는 장치에 내재하는 것이 아닌 다른 구성요소를 포함할 수 있다. 또한, 명백히 반대로 기재되지 않는 한, "또는"은 포괄적인 또는을 지칭하며 배타적인 "또는"을 지칭하는 것이 아니다. 예를 들어, 조건 A 또는 B는 다음 중 어느 하나에 의해 충족된다: A는 참이고(또는 존재하고) B는 거짓임(또는 존재하지 않음), A는 거짓이고(또는 존재하지 않고) B는 참임(또는 존재함), 및 A 및 B 둘 다가 참임(또는 존재함).
본 명세서에서 사용되는 바와 같이, 다수의 열거된 구성요소 사이의 접속 용어 "및/또는"은 개별 및 조합 옵션 둘 모두를 포함하는 것으로 이해된다. 예를 들어, 두 구성요소가 "및/또는"에 의해 결합될 경우, 첫 번째 옵션은 제2 구성요소없이 제1 구성요소의 적용 가능성을 지칭한다. 두 번째 옵션은 제1 구성요소없이 제2 구성요소의 적용 가능성을 지칭한다. 세 번째 옵션은 제1 및 제2 구성요소 둘 다의 적용가능성을 지칭한다. 이들 옵션 중 어느 것이든 그 의미 내에 속하며 따라서 본 명세서에서 사용되는 용어 "및/또는"의 요건을 충족하는 것으로 이해된다. 옵션 중 하나 보다 많은 것의 동시 적용 가능성도 그 의미 내에 속하며 따라서 용어 "및/또는"의 요건을 충족하는 것으로 이해된다.
본 명세서에서 사용되는 바와 같이, 본 명세서 및 청구범위 전체에 걸쳐 사용되는 바와 같은 "~으로 이루어지다" 또는 변형, 예컨대 "~으로 이루어진다" 또는 "~으로 이루어진"이라는 용어는 임의의 인용된 정수 또는 정수들의 군을 포함함을 나타내며, 추가의 정수 또는 정수들의 군은 명시된 방법, 구조, 또는 조성물에 부가될 수 없음을 나타낸다.
본 명세서에서 사용되는 바와 같이, 본 명세서 및 청구범위 전체에 걸쳐 사용되는 바와 같은 "~으로 본질적으로 이루어지다" 또는 변형, 예컨대 "~으로 본질적으로 이루어진다" 또는 "~으로 본질적으로 이루어진"이라는 용어는 임의의 인용된 정수 또는 정수들의 군을 포함함을 나타내며, 명시된 방법, 구조, 또는 조성물의 기본적이거나 신규한 특성을 실질적으로 변화시키지 않는 임의의 열거된 정수 또는 정수들의 군을 선택적으로 포함함을 나타낸다. 문헌[M.P.E.P. §2111.03] 참조.
본 명세서에서 사용되는 "대상체"는 본 발명의 실시형태에 따른 방법에 의해 백신접종되는 동물, 바람직하게는 포유류, 가장 바람직하게는 인간을 의미한다. 본 명세서에서 사용되는 바와 같은 "포유류"라는 용어는 임의의 포유류를 포괄한다. 포유류의 예는 소, 말, 양, 돼지, 고양이, 개, 마우스, 래트, 토끼, 기니피그, 원숭이, 인간 등, 더 바람직하게는 인간을 포함하지만, 이들로 제한되지 않는다.
단어 "우측", "좌측", "하부" 및 "상부"는 언급되는 도면에서의 방향을 표기한다.
바람직한 발명의 구성성분의 치수 또는 특징을 언급할 때 본 명세서에서 사용되는 용어 "약", "대략적으로", "대체적으로", "실질적으로" 등의 용어는 기재된 치수/특성이 엄격한 경계 또는 파라미터가 아니며 기능적으로 동일하거나 유사한 이로부터의 사소한 변화를 배제하는 것이 아님이 또한 이해되어야 하는데, 이는 당업자에 의해 이해되는 바와 같다. 최소한, 수치 파라미터를 포함하는 그러한 언급은, 본 기술분야에서 용인되는 수학적 및 공업적 원리(예를 들어, 반올림, 측정 오차 또는 기타 계통 오차, 제조 공차 등)를 이용할 경우 최하위 유효 숫자를 변화시키지 않는 변화를 포함한다.
둘 이상의 핵산 또는 폴리펩티드 서열(예를 들어, 헥손 및 섬유 폴리펩티드 및 이들을 암호화하는 폴리뉴클레오티드)과 관련하여 "동일한" 또는 "동일성" 백분율이라는 용어는, 다음의 서열 비교 알고리즘 중 하나를 이용하여 측정하여 또는 육안검사에 의해, 최대 대응도에 대해 비교하고 정렬할 때, 둘 이상의 서열 또는 하위서열이 동일하거나 동일한 아미노산 잔기 또는 뉴클레오티드의 구체화된 백분율을 가지는 것을 지칭한다.
서열 비교를 위해, 전형적으로 하나의 서열은 시험 서열과 비교되는 기준 서열로서 작용한다. 서열 비교 알고리즘을 이용할 때, 시험 및 기준 서열은 컴퓨터에 입력되며, 필요하다면 하위 서열 좌표가 표기되고, 서열 알고리즘 프로그램 파라미터가 표기된다. 이어서, 서열 비교 알고리즘은 표기된 프로그램 파라미터에 기반하여 기준 서열에 비한 시험 서열(들)에 대한 서열 동일성 백분율을 계산한다.
비교를 위한 최적의 서열 정렬은, 예를 들어, 문헌[Smith & Waterman, Adv. Appl. Math. 2:482(1981)]의 국소 상동성 알고리즘에 의해, 문헌[Needleman & Wunsch, J. Mol. Biol. 48:443(1970)]의 상동성 정렬 알고리즘에 의해, 문헌[Pearson & Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444(1988)]의 유사성 방법의 검색에 의해, 이들 알고리즘(위스콘신 제네틱스 소프트웨어 패키지(Wisconsin Genetics Software Package)의 GAP, BESTFIT, FASTA 및 TFASTA, 위스콘신주 메디슨 사이언스 드라이브 575에 소재한 제네틱스 컴퓨터 그룹(Genetics Computer Group))의 컴퓨터 실행에 의해, 또는 육안검사에 의해(일반적으로, 문헌[Current Protocols in Molecular Biology, F.M. Ausubel et al., eds., Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (1995 Supplement) (Ausubel)] 참조) 수행될 수 있다.
서열 동일성 및 서열 유사성 백분율을 결정하는 데 적합한 알고리즘의 예는 각각 문헌[Altschul et al. (1990) J. Mol. Biol. 215: 403-410] 및 문헌[Altschul et al. (1997) Nucleic Acids Res. 25: 3389-3402]에 기재되어 있는 BLAST 및 BLAST 2.0 알고리즘이다. BLAST 분석을 수행하기 위한 소프트웨어는 미국 국가생물공학센터(National Center for Biotechnology Information)를 통해 공개적으로 입수 가능하다. 이 알고리즘은 데이터베이스 서열에서 동일한 길이의 단어로 정렬될 때 일부 양의 값 역치 스코어(T)를 매칭하거나 충족시키는 질의 서열에서 길이 W의 짧은 단어를 확인함으로써 고 스코어링 서열 쌍(high scoring sequence pairs: HSP)을 처음에 확인하는 것을 수반한다. T는 이웃 단어 스코어 역치로서 지칭된다(Altschul et al, 상기 참조). 이들 초기 이웃 단어 히트(hit)는 이들을 함유하는 더 긴 HSP를 발견하기 위한 검색을 시작하기 위한 종자로서 작용한다. 이어서, 누적 정렬 스코어가 증가될 수 있는 한, 단어 히트는 각각의 서열을 따라서 양 방향으로 연장된다.
누적 스코어는 뉴클레오티드 서열에 대해, 파라미터 M(매칭 잔기 쌍에 대한 보상 스코어; 항상 0 초과) 및 N(미스매칭 잔기에 대한 페널티 스코어; 항상 0 미만)을 이용하여 계산된다. 아미노산 서열에 대해, 스코어링 매트릭스를 사용하여 누적 스코어를 계산한다. 누적 정렬 스코어가 최대 달성 값으로부터 양 X만큼 하락하고; 하나 이상의 음성-스코어링 잔기 정렬의 누적 때문에 누적 스코어가 0 이하로 될 때 각각의 방향에서 단어 히트의 연장은 중단되거나; 서열 중 하나의 말단에 도달된다. BLAST 알고리즘 파라미터 W, T 및 X는 정렬의 민감도 및 속도를 결정한다. BLASTN 프로그램은 (뉴클레오티드 서열에 대해) 디폴트로서, 단어길이(W) 11, 및 예상치(E) 10, M=5, N=-4, 및 두 가닥의 비교를 사용한다. 아미노산 서열에 대해, BLASTP 프로그램은 디폴트로서 단어길이(W) 3, 예상치(E) 10, 및 BLOSUM62 스코어링 매트릭스를 사용한다(문헌[Henikoff & Henikoff, Proc. Natl. Acad. Sci. USA 89:10915(1989)] 참조).
서열 동일성 백분율을 계산하는 것에 추가로, BLAST 알고리즘은 또한 두 서열 사이의 유사성의 통계학적 분석을 수행한다(예를 들어, 문헌[Karlin & Altschul, Proc. Nat'l. Acad. Sci. USA 90:5873-5787 (1993)] 참조). BLAST 알고리즘에 의해 제공되는 유사성의 한 척도는 두 뉴클레오티드 또는 아미노산 서열 사이의 매치가 우연히 일어날 확률의 표시를 제공하는 가장 적은 합계 확률(P(N))이다. 예를 들어, 시험 핵산과 기준 핵산의 비교에서 가장 작은 합계 확률이 약 0.1 미만, 더 바람직하게는 약 0.01 미만, 가장 바람직하게는 약 0.001 미만이라면, 핵산은 기준 서열과 유사한 것으로 간주된다.
두 핵산 서열 또는 폴리펩티드가 실질적으로 동일하다는 추가적인 표시는 이하에 기재하는 바와 같이 제1 핵산에 의해 암호화된 폴리펩티드가 제2 핵산에 의해 암호화되는 폴리펩티드와 면역학적으로 교차 반응성이라는 것이다. 따라서, 폴리펩티드는 전형적으로 제2 폴리펩티드와 실질적으로 동일하며, 예를 들어, 두 펩티드는 보존적 치환에 의해서만 상이하다. 두 핵산 서열이 실질적으로 동일하다는 다른 표시는 이하에 기재하는 바와 같이 서로 엄격한 조건으로 두 분자가 혼성화하는 것이다.
본 명세서에서 사용되는 바와 같은 용어 "보호 면역성" 또는 "보호 면역 반응"은 백신접종된 대상체가 백신접종이 예방하는 병원체의 감염을 제어할 수 있음을 의미한다. 병원성 제제는, 예를 들어, 항원성 유전자 산물 또는 항원 단백질 또는 이들의 단편일 수 있다. 보통, "보호 면역 반응"이 생긴 대상체는 단지 경증 내지 중등증의 임상 증상이 발생되거나 전혀 증상이 발생되지 않는다. 보통, 특정 제제에 대한 "보호 면역 반응" 또는 "보호 면역성"을 갖는 대상체는 상기 제제에 의한 감염 결과로서 사망하지 않을 것이다.
"아쥬반트"라는 용어는 면역계의 자극을 야기하는 하나 이상의 물질로서 정의된다. 이와 관련하여, 아쥬반트는 본 발명의 아데노바이러스 벡터에 대한 면역 반응을 향상시키기 위해 사용된다.
본 명세서에서 사용되는 "항원성 유전자 산물 또는 이의 단편" 또는 "항원성 단백질"이라는 용어는 박테리아, 바이러스, 기생충 또는 진균 단백질 또는 이들의 단편을 포함할 수 있다. 바람직하게는, 항원성 단백질 또는 항원성 유전자 산물은 숙주에서 보호 면역 반응을 일으킬 수 있고, 예를 들어, 질환 또는 감염(예를 들어, 박테리아, 바이러스, 기생충 또는 진균 질환 또는 감염)에 대해 면역 반응을 유도하고/하거나 질환 또는 감염에 대해 대상체에서 면역을 생성하여(즉, 백신접종되어), 대상체를 질환 또는 감염으로부터 보호할 수 있다.
아데노바이러스 벡터
특정 아데노바이러스에 대한 노출은 아데노바이러스 벡터의 효능에 영향을 미칠 수 있는 특정 아데노바이러스 혈청형에 대한 면역 반응을 초래하였다. 인간 아데노바이러스에 의한 감염은 인간에서 통상적이기 때문에, 인간 집단에서 인간 아데노바이러스에 대한 중화 항체의 유병률은 높다. 개체에서 이러한 중화 항체의 존재는 인간 아데노바이러스 골격에 기반하여 유전자 전달 벡터의 효능을 감소시키는 것으로 예상될 수 있다. 효능 감소를 피하는 한 가지 방법은 중화 항체의 표적인 아데노바이러스 캡시드 단백질 상에서 에피토프를 대체하는 것이다. 캡시드 단백질 상의 표적 서열은 낮은 유병률을 갖는 다른 아데노바이러스로부터의 단백질 서열로 대체될 수 있고, 따라서 이에 대한 중화 항체는 인간 집단에서 희귀하다.
"캡시드 단백질"은 특정 아데노바이러스의 혈청형 및/또는 향성을 결정하는 데 연루된 아데노바이러스(예를 들어, BLY6, HAdV-4)의 캡시드 상의 단백질 또는 이의 기능성 단편 또는 유도체를 지칭한다. 캡시드 단백질은 전형적으로 섬유, 펜톤(penton) 및/또는 헥손 단백질을 포함한다. 특정 실시형태에서, 캡시드 단백질은 아데노바이러스의 전체 또는 전장 캡시드 단백질이다. 다른 실시형태에서, 캡시드 단백질은 아데노바이러스의 전장 캡시드 단백질의 단편 또는 유도체이다. 특정 실시형태에서, 본 발명의 아데노바이러스 벡터에 의해 암호화된 헥손, 펜톤 및 섬유는 동일 또는 상이한 아데노바이러스 배경(즉, BLY6 헥손과 BLY6 섬유, BLY6 헥손과 인간 아데노바이러스 섬유, 인간 아데노바이러스 헥손과 BLY6 섬유 등)을 가진다.
"헥손 폴리펩티드"는 아데노바이러스 헥손 외피 단백질, 이의 기능성 단편 및 유도체를 지칭한다.
"섬유 폴리펩티드"는 아데노바이러스 섬유 단백질, 이의 기능성 단편 및 유도체를 지칭한다.
아데노바이러스에 대한 중화 항체의 하나의 표적은 주요 외피 단백질인 헥손 단백질이다. 혈청형을 정하고 중화 항체에 결합하는 헥손 단백질 또는 헥손 단백질 내의 가변 서열을 본 명세서에 기재된 해당 고릴라 서열과 같이 인간 집단에서 희귀한 아데노바이러스로부터의 헥손 단백질 또는 헥손 단백질 내의 가변 서열로 대체하는 것은 인간에서 통상적으로 발견되는 항체에 의해 중화될 여지가 더 적은 아데노바이러스 벡터의 작제를 가능하게 할 수 있다.
아데노바이러스에 대한 중화 항체의 제2 표적은 섬유 단백질이다. 섬유 단백질 또는 섬유 단백질 내의 가변 서열을 본 명세서에 기재된 해당 고릴라 서열과 같이 인간 집단에서 희귀한 아데노바이러스로부터의 섬유 단백질 또는 섬유 단백질 내의 가변 서열로 대체하는 것은 또한 인간에서 통상적으로 발견되는 항체에 의해 중화될 여지가 더 적은 아데노바이러스 벡터의 작제를 가능하게 할 수 있다. 상기 기재한 섬유 대체와 헥손 대체의 조합은 인간 집단에 통상적으로 존재하는 항체에 의해 중화에 대해 추가적인 내성을 부여할 수 있다.
본 개시내용은 단리된 유인원 아데노바이러스 혈청형으로부터 유래된 헥손 폴리펩티드 및/또는 섬유 폴리펩티드를 암호화하는 단리된 핵산 서열, 및 단리된 핵산 서열 중 적어도 하나를 포함하는 아데노바이러스 벡터를 제공한다.
폴리펩티드의 "기능성 유도체"는 적합하게는 폴리펩티드의 변형된 형태를 지칭하며, 예를 들어, 폴리펩티드의 하나 이상의 아미노산이 결실되고/되거나, 삽입되고/되거나, 변형되고/되거나 치환될 수 있다. 비변형 아데노바이러스 캡시드 단백질의 유도체는, 예를 들어,
(a) 캡시드 내에 유도체 캡시드 단백질을 포함하는 아데노바이러스가 비변형 캡시드 단백질을 포함하는 아데노바이러스에 비교하여 실질적으로 동일하거나 더 낮은 혈청유병률을 보유하고; 그리고/또는
(b) 캡시드 내에 유도체 캡시드 단백질을 포함하는 아데노바이러스가 비변형 캡시드 단백질을 포함하는 아데노바이러스에 비교하여 실질적으로 동일하거나 더 높은 숙주 세포 감염성을 보유하고; 그리고/또는
(c) 캡시드 내에 유도체 캡시드 단백질을 포함하는 아데노바이러스가 비변형 캡시드 단백질을 포함하는 아데노바이러스에 비교하여 실질적으로 동일하거나 더 높은 면역원성을 보유하고; 그리고/또는
(d) 캡시드 내에 유도체 캡시드 단백질을 포함하는 아데노바이러스가 비변형 캡시드 단백질을 포함하는 아데노바이러스에 비교하여 실질적으로 동일하거나 더 높은 수준의 이식유전자 생산성을 보유한다면,
기능성으로 간주된다.
"아데노바이러스 벡터"는 아데노바이러스 게놈의 적어도 일부로부터 유래되거나 이를 함유하는 재조합 벡터를 지칭한다.
바람직한 실시형태에서, 단리된 핵산 서열은 서열번호 1의 아미노산 서열 또는 서열번호 1의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함하는 헥손 폴리펩티드 또는 이의 기능성 유도체를 암호화한다. 특정 실시형태에서, 헥손 폴리펩티드는 서열번호 1의 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함한다. 바람직한 실시형태에서, 헥손 폴리펩티드 또는 이의 기능성 유도체는 BLY6 헥손 폴리펩티드의 아미노산 서열(서열번호 2) 또는 서열번호 2의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다. 특정 실시형태에서, 헥손 폴리펩티드 또는 이의 기능성 유도체는 서열번호 2의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98% 또는 적어도 99% 동일한 아미노산 서열을 포함한다.
바람직한 실시형태에서, 단리된 핵산 서열은 섬유 폴리펩티드 또는 이의 기능성 유도체를 암호화한다. 섬유 폴리펩티드는, 예를 들어, 서열번호 10의 아미노산 서열을 포함하는 섬유 노브 폴리펩티드; 서열번호 11의 아미노산 서열을 포함하는 섬유 샤프트 폴리펩티드; 및 서열번호 12의 아미노산 서열을 포함하는 섬유 꼬리 폴리펩티드 중 적어도 하나를 포함할 수 있다. 바람직한 실시형태에서, 섬유 폴리펩티드 또는 이의 기능성 유도체는 BLY6 섬유 폴리펩티드의 아미노산 서열(서열번호 3) 또는 서열번호 3의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다.
바람직한 실시형태에서, 본 명세서에 개시된 헥손 폴리펩티드 중 적어도 하나를 암호화하는 헥손 핵산 서열, 및 본 명세서에 개시된 섬유 폴리펩티드 중 적어도 하나를 암호화하는 핵산 서열을 포함하는 단리된 핵산이 제공된다.
바람직한 실시형태에서, 본 발명의 실시형태에 따른 단리된 헥손 핵산 서열 및/또는 단리된 섬유 핵산 서열 중 적어도 하나를 포함하는 벡터, 바람직하게는 아데노바이러스 벡터가 제공된다. 아데노바이러스 벡터는, 예를 들어, 적어도 하나의 이식유전자; 및 헥손 폴리펩티드 및/또는 섬유 폴리펩티드를 암호화하는 핵산 서열을 포함할 수 있되, 헥손 폴리펩티드는 본 명세서에 개시된 헥손 폴리펩티드 초가변영역-포함 폴리펩티드를 포함하는 폴리펩티드를 포함하고, 섬유 폴리펩티드는 본 명세서에 기재된 섬유 폴리펩티드를 포함할 수 있다.
전형적으로, 본 발명의 아데노바이러스 벡터는, 예를 들어, 플라스미드, 코스미드 또는 바큘로바이러스 벡터 상에 전체 재조합 아데노바이러스 게놈을 포함한다. 본 발명의 핵산 분자는 클로닝에 의해 수득되거나 합성에 의해 생성되는 RNA 형태 또는 DNA 형태로 존재할 수 있다. DNA는 이중 가닥 또는 단일 가닥일 수 있다.
당업자는 다중 혈청형으로부터 유래된 구성요소가 단일 아데노바이러스 벡터, 예를 들어 인간 또는 유인원 아데노바이러스에서 조합될 수 있다는 것을 인식할 것이다. 따라서, 상이한 혈청형으로부터의 바람직한 특성을 조합하는 키메라 아데노바이러스 벡터가 생산될 수 있다. 따라서, 일부 실시형태에서, 본 발명의 키메라 아데노바이러스 벡터는 유인원 헥손 및/또는 섬유 폴리펩티드 서열의 이미 존재하는 면역의 부재를 존재하는 아데노바이러스 벡터, 예컨대 rAd4, rAd5, rAd26 또는 rAd35의 고수준의 항원 전달 및 제시 능력과 조합할 수 있었다.
백신으로서 사용하기 위한 아데노바이러스의 이점은 보고된 수많은 아데노바이러스 벡터의 연구, 개발, 제조 및 임상 시험에서의 다년간의 경험을 기반으로 한 조작의 용이성, 양호한 대규모 제조 가능성, 및 우수한 안전성 기록을 포함한다. 일반적으로 백신으로서 사용되는 아데노바이러스 벡터는 세포성 면역 반응을 비롯한, 이식유전자-암호화된 단백질에 대한 양호한 면역 반응을 제공한다. 본 발명에 따른 아데노바이러스 벡터는 임의의 유형의 아데노바이러스를 기반으로 할 수 있고, 특정 실시형태에서 임의의 그룹 또는 혈청형을 가질 수 있는 인간 아데노바이러스이다. 바람직한 실시형태에서, 재조합 아데노바이러스는 그룹 A, B, C, D, E, F 또는 G로부터의 인간 아데노바이러스에 기반한다. 다른 바람직한 실시형태에서, 재조합 아데노바이러스는 인간 아데노바이러스 혈청형 5, 11, 26, 34, 35, 48, 49 또는 50을 기반으로 한다. 다른 실시형태에서, 이는 임의의 혈청형을 가질 수 있는, 침팬지 또는 고릴라 아데노바이러스와 같은 유인원 아데노바이러스이다. 특정한 실시형태에서, 재조합 아데노바이러스는 침팬지 아데노바이러스 1형, 3형, 7형, 8형, 21형, 22형, 23형, 24형, 25형, 26형, 27.1형, 28.1형, 29형, 30형, 31.1형, 32형, 33형, 34형, 35.1형, 36형, 37.2형, 39형, 40.1형, 41.1형, 42.1형, 43형, 44형, 45형, 46형, 48형, 49형, 50형, 67형 또는 SA7P형을 기반으로 한다.
더 바람직한 실시형태에서, 제2 조성물의 침팬지 아데노바이러스 벡터는 ChAdV3이다. 재조합 침팬지 아데노바이러스 혈청형 3(ChAd3 또는 cAd3)은 인간 아데노바이러스 혈청형 5(Ad5)와 유사한 특성을 갖는 하위그룹 C 아데노바이러스이다. ChAd3은 C형 간염 바이러스(HCV)에 대해 후보 백신을 평가하는 인간 연구에서 안전하고 면역원성인 것으로 나타났다(Barnes E, et al. 2012 Science translational medicine 4: 115ra1). ChAd3-기반 백신은 인간 Ad5 벡터 백신과 비슷한 면역 반응을 유도할 수 있다는 것이 보고되었다. 예를 들어, 문헌[Peruzzi D, et al. 2009 Vaccine 27: 1293-300] 및 문헌[Quinn KM, et al. 2013 J Immunol 190: 2720-35]; WO 2005/071093; WO2011/0130627 등을 참조.
아데노바이러스 벡터, 이의 작제 방법 및 이의 증식 방법은 당업계에 잘 공지되어 있고, 예를 들어, 미국 특허 제5,559,099호, 제5,837,511호, 제5,846,782호, 5,851,806호, 제5,994,106호, 제5,994,128호, 제5,965,541호, 제5,981,225호, 제6,040,174호, 6,020,191호 및 제6,113,913호, 및 문헌[Thomas Shenk, "Adenoviridae and their Replication", M. S. Horwitz, "Adenoviruses", 각각 67 및 68장, Virology, B. N. Fields et al., eds., 3d ed., Raven Press, Ltd., New York (1996)], 및 본 명세서에 언급된 다른 참고문헌에 기재되어 있다. 전형적으로, 아데노바이러스 벡터의 작제는 표준 분자 생물학 기술, 예컨대 문헌[ambrook et al., Molecular Cloning, a Laboratory Manual, 2d ed., Cold Spring Harbor Press, Cold Spring Harbor, N.Y. (1989)], 문헌[Watson et al., Recombinant DNA, 2d ed., Scientific American Books (1992)], 및 문헌[Ausubel et al., Current Protocols in Molecular Biology, Wiley Interscience Publishers, NY (1995)], 및 본 명세서에 언급된 다른 참고문헌에 기재된 것의 사용을 포함한다.
특정 실시형태에서, 아데노바이러스 벡터는 E1 결실 및/또는 E3 결실을 포함한다. E1 또는 E3 결실은, 예를 들어, E1 또는 E3 유전자 산물에 기능적 결함을 제공하는, 유전자의 완전한 결실 또는 부분적 결실을 포함할 수 있다. 따라서, 특정 실시형태에서 아데노바이러스는, 예를 들어, 게놈의 E1 영역에서의 결실을 포함하기 때문에, 복제 결함성이다. 당업자에게 알려진 바와 같이, 아데노바이러스 게놈으로부터의 필수 영역의 결실의 경우, 이들 영역에 의해 암호화되는 기능은 바람직하게는 생산자 세포에 의해 도중에 제공되어야 하며, 즉, E1, E2 및/또는 E4 영역의 일부 또는 전체가 아데노바이러스로부터 결실될 때, 이들은 생산자 세포에 존재해야 하거나, 예를 들어, 이의 게놈에 통합되어야 하거나, 또는 소위 헬퍼 아데노바이러스 또는 헬퍼 플라스미드의 형태로 존재하여야 한다. 아데노바이러스는 또한 복제에 불필요한 E3 영역에서의 결실을 가질 수 있고, 따라서 이러한 결실은 보완될 필요가 없다. E1, E2, E3 및 E4 영역 중 하나 이상은 또한 다른 수단에 의해, 예컨대 관심 대상의 이식유전자(보통 프로모터에 연결됨)를 비활성화될 영역 내로 삽입함으로써 비활성화될 수 있다.
사용될 수 있는 생산자 세포(때때로 당업계 및 본 명세서에서 '패키징 세포' 또는 '상보성 세포'로도 지칭됨)는 목적하는 아데노바이러스가 증식될 수 있는 임의의 생산자 세포일 수 있다. 예를 들어, 재조합 아데노바이러스 벡터의 증식은 아데노바이러스의 결함을 보완하는 생산자 세포에서 행해진다. 이러한 생산자 세포는 바람직하게는 이들의 게놈에 적어도 아데노바이러스 E1 서열을 가지며, 이에 의해 E1 영역에서 결실을 갖는 재조합 아데노바이러스를 보완할 수 있다. E1에 의하여 불멸화된 인간 망막 세포, 예컨대, 911 또는 PER.C6 세포(미국 특허 제5,994,128호 참조), E1-형질전환된 양수세포(유럽 특허 제1230354호 참조), E1-형질전환된 A549 세포(예컨대, WO 98/39411, 미국 특허 번호 제5,891,690호 참조), GH329:HeLa(Gao 등, 2000, Hum Gene Ther 11: 213-19), 293 등과 같은 임의의 E1-상보성 생산자 세포가 이용될 수 있다. 특정 실시형태에서, 생산자 세포는, 예를 들어 HEK293 세포, 또는 PER.C6 세포, 또는 911 세포, 또는 IT293SF 세포 등이다. 생산자 세포에서의 아데노바이러스 벡터의 생산은 (Kovesdi et al., 2010, Viruses 2:1681-703)에서 검토된다.
특정 실시형태에서, 아데노바이러스 벡터는 하나 이상의 인간 아데노바이러스 핵산 서열을 포함하는 키메라 아데노바이러스 벡터이다. 인간 아데노바이러스 핵산은, 예를 들어, 인간 아데노바이러스-4(Ad-4), 인간 아데노바이러스-5(Ad-5), 인간 아데노바이러스-26(Ad-26) 또는 인간 아데노바이러스-35(Ad-35)로부터 선택될 수 있다. 특정 실시형태에서, E1-결함 아데노바이러스 벡터는 인간 Ad5의 아데노바이러스의 E4-orf6 암호화 서열을 포함한다. 이는, 예를 들면, 293 세포 또는 PER.C6 세포와 같이, Ad5의 E1 유전자를 발현시키는 잘 공지된 상보성 세포주에서 이러한 아데노바이러스의 증식을 가능하게 한다(예컨대, 본 명세서에 전문이 참고로 포함된 문헌[Fallaux et al., 1998, Hum Gene Ther 9: 1909-17, Havenga et al., 2006, J Gen Virol 87: 2135-43]; WO 03/104467 참조).
특정 실시형태에서, 아데노바이러스 벡터는 이식유전자를 포함한다. "이식유전자"는 벡터에 자연적으로 존재하지 않는 핵산인 이종성 핵산을 지칭하며, 본 발명에 따르면 이식유전자는 대상체에서 면역 반응을 유발하는 항원성 유전자 산물 또는 항원성 단백질을 암호화할 수 있다. 이식유전자는, 예를 들어, 표준 분자 생물학 기법에 의해 벡터 내로 도입될 수 있다. 이식유전자는, 예를 들어 아데노바이러스 벡터의 결실된 E1 또는 E3 영역 내로, 또는 E4 영역과 rITR 사이의 영역에 클로닝될 수 있다. 이식유전자는 일반적으로 발현 제어 서열에 작동 가능하게 연결된다. 바람직한 실시형태에서, 이식유전자는 이식유전자 삽입 부위에 삽입된다.
필요하다면, 본 발명의 실시형태에 따른 헥손 및/또는 섬유 폴리펩티드를 암호화하는 핵산 서열, 및/또는 이식유전자는 치료되는 숙주(예를 들어, 인간)에서 적절한 발현을 보장하도록 코돈-최적화될 수 있다. 코돈-최적화는 본 기술 분야에서 널리 적용되는 기술이다.
이식유전자는 아데노바이러스-유래 프로모터(예를 들어, 주요 후기 프로모터)의 제어 하에 있을 수 있거나(즉, 이에 작동 가능하게 연결될 수 있거나) 또는 이종성 프로모터의 제어 하에 있을 수 있다. 적합한 이종성 프로모터의 예는 CMV 프로모터 및 RSV 프로모터를 포함한다. 바람직하게는, 프로모터는 발현 카세트 내에서 관심 대상의 이종 유전자 상류에 위치된다.
바람직한 실시형태에서, 아데노바이러스 벡터는 서열번호 13 또는 서열번호 14의 핵산 서열을 포함한다.
면역원성 조성물
면역원성 조성물은 본 발명에서 사용하기 위한 면역학적 유효량의 정제된 또는 부분적으로 정제된 인간 또는 유인원(예를 들어, 고릴라) 아데노바이러스 벡터를 포함하는 조성물이다. 상기 조성물은 당업계에 잘 공지된 방법에 따라 백신(또한 "면역원성 조성물"로서 지칭됨)으로서 제형화될 수 있다. 이러한 조성물은 면역 반응을 향상시키기 위해 아쥬반트를 포함할 수 있다. 제형 내 각각의 성분의 최적 비는 본 개시내용을 고려하여 당업자에게 잘 공지된 기법에 의해 결정될 수 있다.
본 발명의 실시형태에 따른 면역원성 조성물은 본 개시내용을 고려하여 당업자에게 공지된 방법을 이용하여 생산될 수 있다. 액체 약제학적 조성물은 일반적으로 액체 담체, 예를 들어 물, 석유, 동물성 또는 식물성 오일, 광유 또는 합성 오일을 포함한다. 생리 식염수, 덱스트로스 또는 다른 당류 용액 또는 글리콜, 예를 들어 에틸렌글리콜, 프로필렌 글리콜 또는 폴리에틸렌 글리콜이 포함될 수 있다.
본 발명에서 유용한 면역원성 조성물은 아쥬반트를 포함할 수 있다. 본 발명에 따른 공동 투여에 적합한 아쥬반트는 QS-21, 디톡스-PC(Detox-PC), MPL-SE, MoGM-CSF, 타이터맥스-G(TiterMax-G), CRL-1005, GERBU, TER아미드, PSC97B, 아쥬머(Adjumer), PG-026, GSK-I, AS01, AS03, AS04, AS15, GcMAF, B-알레틴, MPC-026, 아쥬백스(Adjuvax), CpG ODN, 베타펙틴, 명반 및 MF59를 비롯하여, 사람에서 잠재적으로 안전하고, 잘 용인되며 유효한 것이어야 한다.
투여될 수 있는 다른 아쥬반트는 렉틴, 성장 인자, 사이토카인 및 림포카인, 예컨대 알파-인터페론, 감마 인터페론, 혈소판 유래 성장 인자(PDGF), 과립구-집락 자극 인자(gCSF), 과립구 대식세포 집락 자극 인자(gMCSF), 종양 괴사 인자(TNF), 내피 성장 인자(EGF), IL-I, IL-2, IL-4, IL-6, IL-8, IL-10 및 IL-12 또는 이들에 대해 암호화하는 핵산을 포함한다.
본 발명의 조성물은 약제학적으로 허용 가능한 부형제, 담체, 완충제, 안정제 또는 당업자에게 잘 공지된 다른 물질을 포함할 수 있다. 이러한 물질은 비독성이며, 활성 성분의 효능을 방해하여서는 안 된다. 담체 또는 기타 물질의 정확한 특성은 투여 경로, 예를 들어 근육내, 피하, 경구, 정맥내, 피부, 점막내(예를 들어, 소화관), 비강내 또는 복강내 경로에 따라 다를 수 있다.
보호 면역을 유도하기 위한 방법
본 발명의 다른 일반적 양상은 면역 반응의 유도가 필요한 대상체에서 면역 반응을 유도하는 방법에 관한 것이다. 상기 방법은, 예를 들어, 본 명세서에 기재된 아데노바이러스 벡터 및 약제학적으로 허용 가능한 담체를 포함하는 백신을 대상체에게 투여하는 단계를 포함할 수 있다. 또한 본 명세서에서 백신의 생산 방법이 제공된다. 상기 방법은 본 명세서에 기재된 아데노바이러스 벡터를 약제학적으로 허용 가능한 담체와 조합하는 단계를 포함한다.
본 명세서에 기재된 것을 포함하지만, 이들로 제한되지 않는 본 발명의 실시형태에 따른 면역원성 조성물 중 어느 것이라도 본 발명의 방법에서 백신으로서 사용될 수 있다.
벡터를 포함하는 면역원성 조성물/백신의 투여는 전형적으로 근육내 또는 피하로 이루어진다. 그러나 다른 방식의 투여, 예컨대 정맥내, 피부, 진피내 또는 비강이 마찬가지로 고려될 수 있다. 면역원성 조성물의 근육내 투여는 아데노바이러스 벡터의 현탁액을 주사하기 위해 바늘을 이용하여 달성될 수 있다. 대안으로 백신을 함유하는 동결-건조 분말 또는 조성물을 투여하기 위한 무바늘 주사 장치의 사용(예를 들어, 바이오젝터(Biojector)TM의 사용)이 있다.
정맥내, 피부 또는 피하 주사 또는 고통 부위에서의 주사를 위해, 벡터는 무발열원이며 적합한 pH, 등장성 및 안정성을 갖는 비경구로 허용 가능한 수용액의 형태일 수 있다. 당업자는, 예를 들어, 등장성 비히클, 예컨대 염화나트륨 주사, 링거 주사액, 젖산 링거 주사액을 이용하여 적합한 용액을 제조하는 것이 용이할 수 있다. 필요하다면, 보존제, 안정제, 완충제, 항산화제 및/또는 다른 첨가제가 포함될 수 있다. 서방출 제형이 또한 사용될 수 있다.
전형적으로, 투여는 감염 또는 증상의 발생 전에 관심 대상의 항원(예를 들어, 박테리아, 바이러스, 기생충 및/또는 진균 병원균)에 대해 면역 반응을 생성하기 위한 예방적 목적을 가질 것이다. 본 발명에 따라 치료되거나 예방될 수 있는 질환 및 장애는 면역 반응이 예방적 또는 치료적 역할을 할 수 있는 것을 포함한다. 다른 실시형태에서, 아데노바이러스 벡터는 노출-후 예방을 위해 투여될 수 있다.
인간 또는 유인원(예를 들어, 고릴라) 아데노바이러스 벡터를 함유하는 면역원성 조성물은 대상체에게 투여되어, 대상체에서 관심 대상의 항원에 대해 면역 반응을 일으킨다. 검출 가능한 면역 반응을 유도하는 데 충분한 조성물의 양은 "면역학적 유효 용량" 또는 "유효량"의 조성물인 것으로 정의된다. 본 발명의 면역원성 조성물은 체액성 면역 반응뿐만 아니라 세포-매개 면역 반응을 유도할 수 있다. 전형적인 실시형태에서, 면역 반응은 보호 면역 반응이다.
실제 투여되는 양, 및 투여 속도 및 시간-과정은 치료 중인 것의 특성 및 중증도에 따라 정해질 것이다. 치료의 처방, 예를 들어, 투약량 등의 결정은 일반적 실행자 및 기타 의사, 또는 수의학과 관련하여 수의사의 책임 내에 있으며, 전형적으로 치료될 장애, 개개 환자의 병태, 전달 부위, 투여 방법 및 실행자에게 공지된 기타 인자를 고려한다. 상기 언급한 기법 및 프로토콜의 예는 문헌[Remington's Pharmaceutical Sciences, 16th edition, Osol, A. ed., 1980]에서 찾을 수 있다.
아데노바이러스 벡터의 생산 및 조성물 내로 이러한 입자의 선택적 제형화 후에, 벡터는 개체, 특히 인간 또는 다른 영장류에게 투여될 수 있다. 투여는 인간 또는 다른 포유류, 예를 들어, 마우스, 래트, 햄스터, 기니피그, 토끼, 양, 염소, 돼지, 말, 소, 당나귀, 원숭이, 개 또는 고양이에 대한 투여일 수 있다. 비-인간 포유류에 대한 전달은 치료적 목적을 위해 필요한 것이 아니라, 실험 목적, 예를 들어, 아데노바이러스 벡터에 대한 면역 반응의 메커니즘 연구에서 사용하기 위한 것일 수 있다.
일 예시적 요법에서, 아데노바이러스 벡터는 약 104 내지 1012개의 바이러스 입자/㎖의 농도를 함유하는 약 100 ㎕ 내지 약 10 ㎖ 범위의 용적으로 (예를 들어, 근육내로) 투여된다. 바람직하게는, 아데노바이러스 벡터는 0.1 내지 2.0 ㎖ 범위의 용적으로 투여된다. 예를 들어, 아데노바이러스 벡터는 100 ㎕, 500 ㎕, 1 ㎖, 2㎖로 투여될 수 있다. 더 바람직하게는, 아데노바이러스 벡터는 0.5 ㎖의 용적으로 투여된다. 선택적으로, 아데노바이러스 벡터는 약 107개의 vp/㎖, 108개의 vp/㎖, 109개의 vp/㎖, 1010개의 vp/㎖, 5×1010개의 vp/㎖, 1011개의 vp/㎖ 또는 1012개의 vp/㎖의 농도로 투여될 수 있다. 전형적으로, 아데노바이러스 벡터는 1회 투여 동안 인간 대상체에게 약 109 내지 약 1012개의 바이러스 입자(vp), 더 전형적으로는 약 1010 내지 약 1012개의 vp의 양으로 투여된다.
초기 백신접종 다음에 관심 대상의 항원을 암호화하는 동일한 아데노바이러스 벡터를 포함하는 백신/조성물 또는 관심 대상의 동일한 항원을 암호화하는 상이한 아데노바이러스 벡터를 포함하는 백신/조성물로부터의 부스트(boost) 또는 킥(kick)이 이어질 수 있다.
조성물은 원한다면 활성 성분을 함유하는 하나 이상의 단위 투약량 형태를 수용할 수 있는 키트, 팩 또는 디스펜서로 제공될 수 있다. 키트는, 예를 들어, 금속 또는 플라스틱 호일, 예컨대 블리스터 팩을 포함한다. 키트, 팩 또는 디스펜서는 투여를 위한 설명서를 수반할 수 있다.
본 발명의 조성물은 치료될 병태에 따라서 단독으로 또는 다른 치료와 병용하여 동시에 또는 순차적으로 투여될 수 있다.
실시형태
본 발명은 또한 다음의 비제한적 실시형태를 제공한다.
실시형태 1은 서열번호 1의 아미노산 서열 또는 서열번호 1의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함하는 헥손 폴리펩티드 또는 이의 기능성 유도체를 암호화하는, 단리된 핵산 서열이다.
실시형태 2는 서열번호 1의 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함하는 헥손 폴리펩티드를 암호화하는, 단리된 핵산 서열이다.
실시형태 3은 실시형태 1 내지 2 중 어느 하나의 단리된 핵산 서열이되, 헥손 폴리펩티드 또는 이의 기능성 유도체는 BLY6 헥손 폴리펩티드의 아미노산 서열(서열번호 2) 또는 서열번호 2의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다.
실시형태 4는 실시형태 1 내지 3의 핵산 서열 중 어느 하나를 포함하는 단리된 핵산 서열이고, 섬유 폴리펩티드 또는 이의 기능성 유도체를 암호화하는 단리된 핵산 서열을 더 포함한다.
실시형태 5는 실시형태 4의 단리된 핵산 서열이되, 섬유 폴리펩티드는 서열번호 10의 아미노산 서열을 포함하는 섬유 노브 폴리펩티드 서열을 포함한다.
실시형태 6은 실시형태 4의 단리된 핵산 서열이되, 섬유 폴리펩티드는 서열번호 11의 아미노산 서열을 포함하는 섬유 샤프트 폴리펩티드 서열을 포함한다.
실시형태 7은 실시형태 4의 단리된 핵산 서열이되, 섬유 폴리펩티드는 서열번호 12의 아미노산 서열을 포함하는 섬유 꼬리 폴리펩티드 서열을 포함한다.
실시형태 8은 실시형태 4 내지 7 중 어느 하나의 단리된 핵산 서열이되, 섬유 폴리펩티드 또는 이의 기능성 유도체는 BLY6 섬유 폴리펩티드의 아미노산 서열(서열번호 3) 또는 서열번호 3의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함한다.
실시형태 9는 섬유 노브 폴리펩티드 서열을 포함하는 섬유 폴리펩티드를 암호화하는 단리된 핵산 서열이되, 섬유 노브 폴리펩티드 서열은 서열번호 10의 아미노산 서열을 포함한다.
실시형태 10은 섬유 샤프트 폴리펩티드 서열을 포함하는 섬유 폴리펩티드를 암호화하는 단리된 핵산 서열이되, 섬유 샤프트 폴리펩티드 서열은 서열번호 11의 아미노산 서열을 포함한다.
실시형태 11은 섬유 꼬리 폴리펩티드 서열을 포함하는 섬유 폴리펩티드를 암호화하는 단리된 핵산 서열이되, 섬유 꼬리 폴리펩티드 서열은 서열번호 12의 아미노산 서열을 포함한다.
실시형태 12는 실시형태 9 내지 11 중 어느 하나의 단리된 핵산 서열이되, 섬유 폴리펩티드는 BLY6 섬유 폴리펩티드의 아미노산 서열(서열번호 3)을 포함한다.
실시형태 13은 실시형태 1 내지 12 중 어느 하나의 핵산을 포함하는 벡터이다.
실시형태 14는 아데노바이러스 벡터이고, 이식유전자를 더 포함하는, 실시형태 13의 벡터이다.
실시형태 15은 실시형태 13 또는 14의 벡터를 포함하는 재조합 세포이다.
실시형태 16은 (a) 벡터의 생산을 위한 조건 하에 실시형태 15의 재조합 세포를 성장시키는 단계; 및 (b) 재조합 세포로부터 벡터를 단리시키는 단계를 포함하는, 벡터의 생산 방법이다.
실시형태 17은 실시형태 14의 벡터를 포함하는 면역원성 조성물이다.
실시형태 18은 대상체에게 실시형태 17의 면역원성 조성물을 투여하는 단계를 포함하는, 면역 반응의 유도가 필요한 대상체에서의 면역 반응 유도 방법이다.
실시형태 19는 (a) 적어도 하나의 이식유전자; 및 (b) 서열번호 1의 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함하는 헥손 폴리펩티드를 암호화하는 핵산 서열을 포함하는, 아데노바이러스 벡터이다.
실시형태 20은 (a) 적어도 하나의 이식유전자; 및 (b) BLY6 헥손 폴리펩티드의 아미노산 서열(서열번호 2)을 포함하는 헥손 폴리펩티드를 암호화하는 핵산 서열을 포함하는 아데노바이러스 벡터이다.
실시형태 21은 (a) 적어도 하나의 이식유전자; BLY6 헥손 폴리펩티드의 아미노산 서열(서열번호 2)을 포함하는 헥손 폴리펩티드를 암호화하는 핵산 서열; 및 (c) BLY6 섬유 폴리펩티드의 아미노산 서열(서열번호 3)을 포함하는 섬유 폴리펩티드를 암호화하는 핵산 서열을 포함하는, 아데노바이러스 벡터이다.
실시예
실시예 1: 신규한 아데노바이러스 단리물 BLY6에 기반한 E1- 및 E3-결실 벡터의 생산
신규한 고릴라 아데노바이러스 단리물인 BLY6(또한 JAd1-WT로 표기함)을 확인하고 서열분석하였다. 이 고릴라 아데노바이러스 단리물은 계통 발생학적으로 인간 아데노바이러스 종 E(HAdV-E)에 속하는 것을 발견하였다. BLY6의 전체 게놈 뉴클레오티드 서열은 서열번호 5인 것으로 결정하였다.
BLY6-기반 Ad 벡터의 생산을 위해 사용한 단일 플라스미드 시스템의 설명
pBLY6.dE1.dE3(서열번호 8; 도 5) 및 pBLY6.dE1.dE3.5IXP(서열번호 9; 도 6)는 단리물 BLY6에 기반한 전장, E1- 및 E3-결실 아데노바이러스 벡터 게놈을 운반하는 플라스미드이다. 이들 플라스미드 내에 함유된 Ad 벡터 게놈 서열은 각각 서열번호 13 및 서열번호 14로 제시된다. 각각의 이들 플라스미드 내에서, 아데노바이러스 벡터 게놈은 2개의 SwaI 제한 효소 부위에 측접된다(즉, 하나의 SwaI 부위는 벡터 게놈의 말단 중 하나에 위치된다). 이들 SwaI 부위는 적합한 E1-상보성 세포(예컨대 HEK293, 911 및 PER.C6 세포)의 형질감염에 의한 바이러스 구조(rescue) 전에 플라스미드 골격으로부터 Ad 벡터 게놈의 절단을 용이하게 하는 것을 의미한다. 이들 플라스미드에 의해 포함되는 Ad 벡터 게놈은 E1 결실 위치에서, E3 결실에서, 그리고 우측 반전 말단 반복부(RITR)에 인접하여 도입된 특정 제한 효소 부위를 추가로 운반한다. 이들 제한 효소 부위는 완전한 Ad 게놈 플라스미드와 관련하여 독특하게 되도록 선택하였다. 그들은 임의의 상기 각각의 위치 또는 이들의 임의의 조합에 삽입된 하나 이상의 이식유전자 발현 카세트를 운반하는 Ad 벡터의 표준 분자 클로닝 기법에 의해 손쉬운 작제를 가능하게 하는 "이식유전자 삽입 부위"를 나타낸다. Ad 벡터 설계 및 플라스미드 작제는 이하의 부문에서 더 상세하게 기재한다.
BLY6-기반 Ad 벡터 게놈 설계
BLY6-기반 Ad 벡터 게놈을 각각 E1 결실, E3 결실, 상이한 이식유전자 삽입 부위, 및 천연 E4 오픈 리딩 프레임(orf) 6 및 orf6/7이 인간 아데노바이러스-5(HAdV-5)의 상기 오픈 리딩 프레임으로 대체되는 것을 포함하도록 설계하였다. 각각의 아데노바이러스의 E1 영역을 결실시키고, AsiSI 제한 효소 부위 서열을 포함하는 이식유전자 삽입 부위로 대체하였다. 각각의 아데노바이러스의 E3 영역을 결실시키고, FseI 제한 효소 부위 서열을 포함하는 이식유전자 삽입 부위로 대체하였다. 각각의 아데노바이러스의 우측 반전 말단 반복부(ITR)에 인접한 PacI 제한 효소 부위 서열의 삽입에 의해 다른 이식유전자 삽입 부위를 생성하였다. E4 orf6 및 orf6/7 암호화 서열을 포함하는 BLY6 서열을 서열번호 6으로 대체하였다. 이들 대체 서열은 인간 아데노바이러스-5(HAdV-5)의 E4 orf6 및 orf6/7 암호화 서열(젠뱅크(GenBank) 서열 AC_000008의 염기쌍 32914 내지 34077)을 포함한다.
2가지 유형의 E1 영역 결실을 설계하고 나서, 작제하였다. pBLY6.dE1.dE3에 의해 포함된 BLY6-기반 Ad 벡터 게놈은 서열번호 5의 뉴클레오티드 453 내지 3016의 제거에 대응하는 E1 영역 결실을 운반한다. 대조적으로, pBLY6.dE1.dE3.5IXP에 의해 포함되는 BLY6-기반 Ad 벡터 게놈은 BLY6의 E1 암호화 서열(즉, 서열번호 5의 뉴클레오티드 453 내지 3366)을 모두 제거하는 더 큰 E1 영역-포함 서열 결실을 운반한다. 더 나아가, HAdV-5에 의한 E1B 55K와 pIX 암호화 서열 사이의 비암호화 서열 신장의 대체를 운반하도록 이 후자의 Ad 벡터 게놈을 추가적으로 설계하였다(즉, 서열번호 5의 뉴클레오티드 3367 내지 3454에 대응하는 서열은 젠뱅크 AC_000008의 뉴클레오티드 3510 내지 3608로(즉, 서열번호 7로) 대체됨).
BLY6-기반 Ad 벡터 게놈을 포함하는 단일 플라스미드의 작제
몇 단계의 유전자 합성(젠스크립트에 의해 수행됨) 및 표준 분자 클로닝 절차에 의해 pBLY6.dE1.dE3(서열번호 8)을 작제하였다. 첫째로, 목적하는 Ad 벡터 게놈(즉, 앞서 언급한 E1 결실을 보유)의 좌측 말단을 함유하는 3586 bp DNA 단편(서열번호 15)을 합성하고 나서, MfeI-NdeI 제한 단편으로서, EcoRI- 및 NdeI-분해된 pBR322(젠뱅크 수탁 번호 - J01749.1)에 결찰시켜, BLY6 중간체 플라스미드 1을 야기하였다. 둘째로, 목적하는 Ad 벡터 게놈(즉, 앞서 언급한 E3 결실, 부분적 E4 서열 대체, 및 rITR에 인접한 이식유전자 삽입 부위를 보유)의 우측 말단을 함유하는 4138 bp 단편(서열번호 16)을 합성하고 나서, BamHI-NdeI 제한 단편으로서, BamHI- 및 NdeI-분해된 BLY6 중간체 플라스미드 1에 결찰시켜, BLY6 중간체 플라스미드 2를 야기하였다. 셋째로, 중간 Ad 벡터 게놈 단편을 함유하는 4563 bp 단편(서열번호 17)을 합성하고 나서, BamHI-MfeI 제한 단편으로서 BamHI- 및 MfeI-분해된 BLY6 중간체 플라스미드 2에 결찰시켜, BLY6 중간체 플라스미드 3(서열번호 18)을 야기하였다. 넷째로, BLY6 바이러스 게놈(서열번호 5)의 18987 bp BsrGI-BsrGI 제한 단편을 BsrGI-분해된 BLY6 중간체 플라스미드 3에 결찰시켜, 최종 플라스미드 pBLY6.dE1.dE3(서열번호 8)을 야기하였다.
상기 언급한 BLY6 중간체 플라스미드 3(서열번호 18)이 목적하는 E1 결실 및 Ad5 pIX 프로모터 삽입을 함유하도록 처음 변형된 것을 제외하고 pBLY6.dE1.dE3.5IXP(서열번호 9)를 pBLY6.dE1.dE3과 동일한 방법으로 작제하였다. 이를 638 bp 단편(서열번호 19)의 합성에 의해 행하고, 후속적으로 이 단편을 AsiSI-FseI 제한 단편으로서, AsiSI- 및 AgeI-분해 BLY6 중간체 플라스미드 3에 결찰시킨다.
pBLY6.FLuc(서열번호 20) 및 pBLY6.RSVF-2A-GLuc(서열번호 21)는 E1 결실 위치에 삽입된 이식유전자 발현 카세트를 구비한 BLY6-기반 Ad 벡터 게놈을 각각 보유하는 pBLY6.dE1.dE3-유래 플라스미드이다. 이들 플라스미드 내에서 운반되는 Ad 벡터 게놈 서열을 각각 서열번호 22 및 서열번호 23에 제시한다. pBLY6.FLuc는 반딧불이 루시퍼라제(FLuc)에 대한 이식유전자 발현 카세트를 운반한다. 이 카세트는 거대세포바이러스 주요 급초기 프로모터(즉, "CMV 프로모터")에 의해 유도되고, SV40-유래 폴리아데닐화 신호를 함유한다. pBLY6.RSVF-2A-Gluc는 호흡기 세포융합 바이러스 균주 A2 융합 당단백질, 구제역 바이러스 2A 펩티드, 및 가우시아 루시퍼라제(GLuc)로 구성된 키메라 단백질인 "RSV-FA2-2A-GLuc"(RSVF-2A-GLuc)에 대한 이식유전자 발현 카세트를 운반한다. Fluc 카세트와 같이, 이 카세트는 CMV 프로모터에 의해 유도되고, SV40 폴리아데닐화 신호를 운반한다. 추가로, 이 카세트는 5' 비번역 영역 내에 인간 아포지질단백질 A1 유전자의 인트론 2를 포함하는 서열을 함유한다. Fluc 및 RSVF-2A-GLuc 발현 카세트를 몇몇 표준 유전자 합성 및 분자 클로닝 단계에 의해 각각 작제하고, 이 후에, 이들을 pBLY6.dE1.dE3의 독특한 AsiSI 제한 효소 부위에 결찰시켜, pBLY6.FLuc 및 pBLY6.RSVF-2A-Gluc을 각각 생산하였다.
BLY6-기반 아데노바이러스 벡터의 생성 및 생산
E1-상보성 PER.C6 세포 내로 대응하는 Ad 벡터 게놈 플라스미드(즉, pBLY6.Fluc 및 pBLY6.RSVF-2A-Gluc)의 형질감염에 의해 아데노바이러스 벡터 게놈 서열(서열번호 22 및 서열번호 23)을 각각 포함하는 아데노바이러스 벡터 BLY6.Fluc(또한 JAd1NVT003으로 표기함) 및 BLY6.RSVF-2A-Gluc(또한 JAd1NVT001로 표기함)를 생산하였다. 10% 소태아 혈청(FBS) 및 10 mM MgCl2로 보충한 둘베코 변형 이글 배지(DMEM)에서 접착 배양물로서 성장시킨 PER.C6 세포 내로 형질감염 전에, Ad 벡터 게놈 플라스미드를 SwaI로 분해시켜 플라스미드로부터 각각의 아데노바이러스 벡터 게놈을 방출시켰다. 리포펙타민 형질감염 시약(캘리포니아주 칼스배드에 소재한 인비트로젠(Invitrogen))을 이용하여 표준 절차에 따라 형질감염을 수행하였다. 바이러스 구조 형질감염의 채취 후에, PER.C6 세포 배양물에 대한 몇몇 연속적 감염 라운드에 의해 바이러스를 추가로 증폭시켰다. 상기 기재한 바와 같은 2-단계 염화세슘(CsCl) 밀도 구배 초원심분리 절차를 이용하여 조질의 바이러스 채취물로부터 바이러스를 정제하였다(Havenga et al., "Novel replication-incompetent adenoviral B-group vectors: high vector stability and yield in PER.C6 cells," J. Gen. Virol. 87(8):2135-43(2006)). 앞서 기재한 분광 측정법-기반 절차에 의해 바이러스 입자(VP) 역가를 측정하였다(Maizel et al., "The polypeptides of adenovirus: I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12," Virology, 36(1):115-25(1968)).
신규한 아데노바이러스 벡터에 의해 유도되는 세포성 면역 반응 및 체액성 면역 반응
실시예 2 및 3은 본 명세서에서 생산된 신규한 BLY6-기반 아데노바이러스 벡터의 면역원성을 평가하기 위해 수행한 실험을 기재한다. 이들 실험에서, 신규한 벡터는 근육내 면역화 후에 벡터-암호화된 (모델) 항원에 대해 체액성 및 세포성 면역 반응을 유도하는 이들의 능력에 대해 평가하였다. 두 상이한 항원을 이용하여 벡터를 시험하였다: 반딧불이 루시퍼라제(FLuc) 및 RSV-FA2-2A-GLuc(RSVF-2A-GLuc). RSVF-2A-GLuc은 호흡기 세포융합 바이러스 균주 A2 융합 당단백질, 구제역 바이러스 2A 펩티드 및 가우시아 루시퍼라제(GLuc)로 구성된 키메라 단백질이다. 동일한 항원-암호화 이식유전자 카세트를 운반하는 인간 아데노바이러스 26형(HAdV-26, 본 명세서에서 Ad26으로도 지칭됨) 또는 인간 아데노바이러스 49형(HAdV-49, 본 명세서에서 Ad49로도 지칭됨)에 기반한 기준 벡터와 각각의 벡터를 함께 비교하였다. 잘 공지된 면역학적 분석, 예컨대 효소-결합 면역흡착 분석(ELISPOT), 효소-결합 면역흡착 분석(ELISA)을 이용하여, RSVF-2A-GLuc 항원의 경우에, 호흡기 세포융합 바이러스 중화 분석(VNA)을 이용하여 각각의 항원에 대한 면역 반응을 측정하였다.
실시예 2: BLY6.FLuc에 의해 유도된 세포성 면역 반응
신규한 아데노바이러스 벡터 BLY6의 세포성 면역원성을 평가하기 위해, Balb/C 마우스를 Ad26.FLuc, Ad49.Fluc(양성 대조군), 반딧불이 루시퍼라제를 발현시키는 BLY6 벡터(BLY6.FLuc)로, 또는 이식유전자를 암호화시키지 않는 아데노벡터(Ad26 빈 벡터(empty))로 근육내 주사에 의해 면역화시켰다. 투여를 위해 두 벡터 용량을 시험하였다: 마우스당 109 및 1010개의 바이러스 입자(vp). 면역화 후 2주에, 마우스를 희생시키고, 비장세포를 15량체 중복 FLuc 펩티드 풀로 밤새 자극하였다(실험 구성 도 1A). IFN-γ-분비 세포의 상대적 수를 측정하는 생체외 ELISPOT 분석에 의해 세포성 면역 반응을 결정하였다(도 1B). 결과는 보다 고용량 면역화(1010)에서, BLY6에 의해 유도되는 세포성 면역 반응이 거의 Ad26.Fluc에 대해 보이는 반응만큼 높다는 것을 나타낸다. 대조적으로, 보다 저용량 면역화(109)에서, BLY6.Fluc는 Ad26.Fluc보다 더 강한 반응을 제공한다.
전반적으로, 본 발명의 FLuc-발현 재조합 BLY6 아데노바이러스 벡터에 의해 유도되는 세포성 면역 반응은 마우스에서 이 벡터의 강한 면역원성을 분명하게 나타낸다.
실시예 3: BLY6.RSVF-2A-GLuc에 의해 유도되는 세포성 및 체액성 면역 반응
벡터-암호화된(모델) 백신 항원으로서 RSV-FA2-2A-GLuc(RSVF-2A-GLuc)를 이용하여 신규한 아데노바이러스 벡터 BLY6의 면역원성을 추가로 평가하였다. Balb/C 마우스를 Ad26.RSVF-2A-GLuc(양성 대조군) 또는 BLY6.RSVF-2A-GLuc로(둘 다 마우스당 108, 109 및 1010개의 바이러스 입자에서), 또는 Ad26.FLuc 또는 BLY6.FLuc로(둘 다 마우스당 1010개의 바이러스 입자) 근육내로 면역화시켰다. 마우스를 8주 후에 희생시키고, 혈액 샘플 및 비장세포를 수집하였다(도 2a). 이하에 기재하는 바와 같이 상이한 면역 파라미터를 평가하였다.
호흡기 세포 융합 바이러스-중화-항체를 유발하는 BLY6.RSVF-2A-GLuc의 능력을 평가하기 위해 바이러스 중화 분석을 수행하였다. 도 2b는 면역화 8주 후에 수집한 혈청 샘플에 대해 측정한 호흡기 세포융합 바이러스 균주 A2(RSV A2) VNA 역가를 도시한다. 각각의 점은 1마리의 마우스를 나타내고, 막대는 그룹 평균을 나타내며, 점선은 정량 하한에 대응한다(LLOQ=6,88; 선형성 샘플의 평균 종말점 역가). 결과는 BLY6.RSVF-2A-GLuc에 의한 1010개의 vp-용량 면역화가 동일한 항원을 암호화하는 기준 Ad26 벡터에 대해 발견되는 것보다 높은 RSV A2 중화 역가를 일으킨다는 것을 나타낸다. BLY6.RSVF-2A-GLuc에 대한 역가는 면역화를 위해 사용한 가장 높은 용량, 즉, 1010개의 vp에서 주로 검출되었다. 예상한 바와 같이, 반딧불이 루시퍼라제를 암호화하는 아데노바이러스에 대해 RSV A2 특이적 반응은 검출되지 않았다.
벡터-암호화된 항원에 대한 세포성 면역의 유도를 RSV-FA2-특이적 ELISPOT 분석에 의해 평가하였다. 이를 위하여, 면역화 8주 후에, 면역화된 마우스로부터의 비장세포를 단리시키고, RSV-FA2 단백질에 걸쳐 있는 15량체 중복 펩티드로 밤새 자극하고 나서, 세포성 면역 반응을 IFN-γ-분비 세포의 상대적 수를 측정하는 생체외 ELISPOT 분석에 의해 결정하였다. 데이터는 RSVF-2A-GLuc를 암호화하는 신규한 벡터 BLY6에 의해 유발된 항원-특이적 세포성 면역 반응이 용량 의존적이고, 용량에 따라, 기준 벡터인 Ad26.RSVF-2A-GLuc에 의해 유도되는 것과 유사한 규모였다는 것을 나타낸다(도 2c). 예상한 바와 같이, 반딧불이 루시퍼라제를 암호화하는 아데노벡터로 면역화된 마우스의 비장세포로부터 측정된 RSVF-FA2-특이적 반응은 없었다.
RSVF-2A-GLuc-발현 벡터가 RSV-FA2-특이적 IgG 항체를 유발하는 능력을 ELISA에 의해 평가하였다. RSVF-2A-GLuc 이식유전자 또는 반딧불이 루시퍼라제(대조군)를 발현시키는 Ad26(양성 대조군) 및 BLY6 벡터로 면역화시킨 마우스로부터의 면역화 후 8주에 수집한 혈청을 항-RSV FA2 IgG 항체 ELISA에서 시험하였다. 구체적으로, 이 ELISA는 재조합 안정한 사전융합 RSV-FA2 단백질(pre-RSV-F)에 결합할 수 있는 IgG 항체를 검출한다. 결과는 BLY6.RSVF-2A-GLuc가 Ad26.RSVF-2A-GLuc에 의해 유도된 것보다 높은 pre-RSV-F-특이적 IgG 항체 역가를 용량 의존적으로 유발한다는 것을 나타낸다(도 2d). 예상한 바와 같이, 반딧불이 루시퍼라제만을 암호화하는 벡터로 면역화된 마우스로부터의 혈청에서 RSV-FA2-특이적 항체 역가는 검출되지 않았다.
종합하면, 데이터는 BLY6 벡터가 HAdV-26에 기반한 기준 벡터에 의해 유도되는 것과 유사한 규모로 암호화된 항원에 대해 강한 세포성 및 체액성 면역 반응을 유도하였다는 것을 나타낸다. 이들 면역 반응은 마우스에서 BLY6 벡터의 강한 면역원성을 분명하게 나타낸다.
실시예 4: 신규한 아데노바이러스 벡터와 기존의 아데노바이러스 벡터 중의 혈청학적 교차-중화의 평가
신규한 아데노바이러스 백신 벡터로서 이들의 잠재적 효용에 대해, 본 명세서에 생산된 신규한 BLY6 아데노바이러스 벡터는 백신 벡터, 예컨대 인간 아데노바이러스 혈청형 HAdV-5 및 HAdV-35에 기반한 벡터로서 현재 이미 개발 중인 기존의 아데노바이러스 벡터와 바람직하게는 혈청학적으로 별개일 것이다. 따라서, 신규한 BLY6 아데노바이러스 벡터 및 HAdV-4, HAdV-5, HAdV-26, HAdV-35 및 HAdV49에 기반한 몇몇 기존의 벡터 중에서 교차-중화 시험을 수행하였다. 이를 위하여, 이들 아데노바이러스 벡터 중 하나에 대해 생긴 마우스 항혈청을 아데노바이러스 중화 분석에서 상이한 벡터 각각에 대하여 시험하였다. 이 분석에 대해 사용한 마우스 항혈청을 마우스당 1010개의 벡터 입자로 마우스의 면역화한 지 2 또는 8주 후에 Balb/C 마우스로부터 수집하였다. 아데노바이러스 중화 분석을 앞서 기재한 바와 같이 수행하였다(Spangers et al 2003. J.Clin. Microbiol. 41:5046-5052). 간략하게, 1:16 희석으로부터 시작하여, 혈청을 2배 연속 희석시키고, 이어서, 반딧불이 루시퍼라제(FLuc)를 발현시키는 아데노바이러스 벡터와 사전 혼합하고 나서, 후속적으로 A549 세포와 밤새 (세포 당 500개 바이러스 입자의 감염 다중도로) 인큐베이션시켰다. 감염 후 24시간에 측정한 감염 세포 용해물에서의 루시퍼라제 활성 수준은 벡터 감염 효율을 나타내었다. 주어진 벡터에 대한 중화 역가를 벡터감염 효율의 90% 감소를 제공할 수 있는 가장 큰 혈청 희석으로서 정하였다. 중화 역가는 임의로 다음의 범주로 나누었다: 16 미만(중화 없음), 16 내지 200, 200 내지 2,000, 및 2,000 초과. 결과는 시험한 벡터 중에 주요 교차-중화가 없다는 것을 나타낸다(도 3). 벡터 BLY6과 Ad26 사이에 단지 일부, 약간의, 일원 교차-중화가 있었으며, BLY6 항혈청은 Ad26에 대한 중화 역가를 16.12(즉, 단지 검출 하한 16을 초과)로 나타내고, Ad26 항혈청은 BLY6에 대한 중화 역가를 나타내지 않았다. 따라서, 신규한 아데노바이러스 벡터 BLY6는 시험 패널에 포함된 인간 아데노바이러스 벡터, 즉, Ad26, Ad35, Ad49, Ad5 및 Ad4와의 교차-중화를 나타내지 않거나, 단지 매우 약간 나타내었다. 따라서, 이 벡터는, 예를 들어 이종성 프라임-부스트 백신접종 요법과 관련하여, 또는 대안적으로 또는 추가적으로, 상이한 질환 또는 항원에 대해 둘 이상의 연속적 백신접종 요법과 관련하여, 순차적 면역화에서 이들 또는 다른 별개의 아데노바이러스 벡터 중 하나 이상과 조합하여 잠재적으로 사용할 수 있었다.
실시예 5: 인간 집단에서 신규한 아데노바이러스 벡터의 혈청유병률
효능있는 백신 벡터로서 이들의 잠재력에 대해 본 명세서에 기재한 신규한 아데노바이러스 벡터가 백신 표적 집단에서 고수준의 이미 존재하는 항-벡터 체액성 면역에 의해 방해되지 않는다는 것이 중요하다. 따라서, BLY6 벡터를 미국(US) 및 유럽연합(EU)에서 18 내지 55세의 성인으로부터의 200개의 인간 코호트 혈청 샘플 내에서 이의 혈청유병률에 대해 평가하였다. 벡터를 실시예 3에서 수행하고 앞서 기재한 바와 같은 표준 아데노바이러스 중화 분석을 수행함으로써 인간 혈청 샘플에 의한 중화에 대해 시험하였다(Sprangers et al 2003. J.Clin. Microbiol. 41:5046-5052]). 간략하게, 1:16 희석으로부터 시작하여, 혈청을 2배 연속 희석시키고, 이어서, 반딧불이 루시퍼라제(FLuc)를 발현시키는 아데노바이러스 벡터와 사전 혼합하고 나서, 후속적으로 A549 세포와 밤새 인큐베이션시켰다(세포 당 500개 바이러스 입자의 감염 다중도로). 감염 후 24시간에 측정한 감염 세포 용해물에서의 루시퍼라제 활성 수준은 벡터 감염 효율을 나타내었다. 주어진 벡터에 대한 중화 역가를 벡터감염 효율의 90% 감소를 제공할 수 있는 가장 큰 혈청 희석으로서 정하였다. 중화 역가는 임의로 다음의 범주로 나누었다: 16 미만(중화 없음), 16 내지 300, 300 내지 1,000, 1000 내지 4000 및 4000 초과.
결과는 BLY6 아데노바이러스 벡터가 대조군 Ad5 벡터 및 기준 Ad26 및 Ad35 벡터보다 연구한 인간 대상체에서 상당히 더 낮은 혈청유병률을 가진다는 것을 나타낸다(도 4). 게다가, 신규한 BLY6 벡터에 대해 보이는 양성 중화 역가는 일반적으로 상당히 낮으며, 대부분 300보다 높지 않았다. 대조적으로, Ad26 및 Ad5에 대해 발견된 대부분의 양성 중화 역가는 300보다 높았다.
종합하면, 상기 데이터는 BLY6 벡터에 대해 이미 존재하는 체액성 항-벡터 면역이 평가한 백신 표적 집단에서 낮은 것으로 간주될 수 있다는 것을 나타내는데, 이는 이들 벡터가 이들 집단에서 효능있는 백신 벡터로서 잠재력을 가진다는 것을 시사한다.
실시예 6: 현탁액 PER.C6 세포에서의 아데노바이러스 벡터 생산성
임상 시험에서 그리고 이후에 사용될 아데노바이러스 벡터는 확장 가능한, 무혈청 아데노바이러스 생산 플랫폼에서 고역가에 대해 용이하게 생산 가능할 필요가 있다. 본 명세서에서 현탁액 PER.C6 세포 또는 sPER.C6로도 지칭되는 현탁액-적합화된 PER.C6®세포는 생물반응기에서 아데노바이러스 벡터의 대규모 제조를 지원하는 것으로 나타났고, 예를 들어, HAdV-26 또는 HAdV-35에 기반한 E1-결실 벡터의 대량의 고역가, 임상 등급 벡터 제조를 달성하기 때문에, 이러한 플랫폼을 나타내었다(유럽 특허 제2536829 B1호, 유럽 특허 제2350268 B1호).
초기 평가로서 본 명세서에 기재된 신규한 벡터가 sPER.C6 세포-기반 생산 공정에 적합한지의 여부에 따라, 진탕 플라스크에서 배양시킨 sPER.C6 세포 상에서 소규모 벡터 생산성 실험을 수행하였다. 실시예 1에 기재한 신규한 Ad 벡터 BLY6의 Fluc-암호화 형태를 이용하여 이들 생산성 실험을 수행하였다. 기준 대조군으로서 HAdV-26-기반 벡터 Ad26.Fluc를 취하였다. 4 mM L-글루타민(론자(Lonza))을 보충한 총 용적 10 ㎖의 퍼멕시스(PERMEXCIS)® 배지(론자로부터 입수 가능)에서 1×106개의 세포/㎖의 밀도로 진탕 플라스크에 파종한 현탁액 PER.C6 세포 배양물을 상이한 바이러스 입자(VP)-대-세포비로 상이한 벡터로 감염시키고, 이어서, 4일 동안 인큐베이션시켰다. 감염을 위해 사용한 상이한 VP-대-세포비는 70, 150 및 900이었다. 감염 세포 배양물의 샘플을 매일 취하고 나서, CMV 프로모터(시험한 모든 벡터에 존재)에 특이적인 프라이머 및 프로브를 사용하는 정량적 PCR(qPCR)-기반 프로토콜에 의해 이들 실험에서 VP 역가를 결정하였다. 이 프로토콜은 임의의 자유 벡터 DNA(즉, 바이러스 입자에 패키징되지 않은 벡터 게놈)를 제거하기 위해 qPCR 전에 시험 샘플의 DNAse 처리를 수반한다.
신규한 벡터 BLY6.Fluc에 대해 얻은 생산성 결과를 도 8에 나타낸다. BLY6.Fluc는 모든 VP-대-세포 감염비 및 시험한 채취 시점에서 기준 대조군 벡터 Ad26.Fluc보다 더 높은 VP 역가를 나타내었다. 이들 결과는 sPER.C6-기반, 무혈청 현탁액 세포 배양물 모델에 대해 신규한 BLY6 벡터의 양호한 생산성을 입증한다.
총괄적으로, 상기 제시한 바와 같은 본 발명의 신규한 재조합 BLY6-기반 아데노바이러스 벡터에 의해 유도되는 체액성 및 세포성 면역 반응의 연구는 마우스에서의 이들 벡터의 강한 면역원성을 분명하게 나타낸다. 추가로, 벡터는 특정의 존재하는 아데노바이러스 백신 벡터 후보(예를 들어, Ad26 및 Ad35)에 대해 교차 중화 항체 반응을 유도하지 않거나, 매우 제한적으로 유도하거나, 또는 그 반대의 경우를 입증하였다. 더 나아가, 신규한 벡터는 인간에서의 낮은 혈청유병률을 나타내었다. 최종적으로, 신규한 벡터는 고수율로 용이하게 생산될 수 있다. 낮은 혈청유병률, 강한 면역원성 및 생산성의 조합은 본 발명의 신규한 아데노바이러스 벡터가 다양한 병원균에 대해 신규한 백신 벡터 후보로서 유용할 수 있고, 추가적으로 유전자 요법 및/또는 진단에서 효용을 가질 수 있다는 것을 시사한다.
광범위한 본 발명의 개념으로부터 벗어나는 일 없이 상기 실시형태에 대한 변화가 이루어질 수 있다는 것이 당업자에 의해 인식될 것이다. 따라서, 본 발명을 개시된 특정 실시형태로 제한하지 않으며, 본 발명의 설명에 의해 정해진 본 발명의 정신과 범주 내에서의 변형을 아우르는 것으로 의도한다는 것이 이해된다.
SEQUENCE LISTING <110> Janssen Vaccines and Prevention B.V. <120> Adenovirus and uses thereof <130> 0297 WO 00 ORD <160> 23 <170> PatentIn version 3.5 <210> 1 <211> 300 <212> PRT <213> Artificial Sequence <220> <223> BLY6 hexon HVRs <400> 1 Lys Glu Asn Asn Gly Gln Gly Asp Ala Lys Thr His Thr Tyr Gly Val 1 5 10 15 Ala Ala Thr Gly Gly Ile Asp Ile Asp Lys Asn Gly Leu Gln Ile Gly 20 25 30 Ile Asp Glu Thr Lys Glu Asp Asn Asn Glu Ile Tyr Ala Asp Lys Thr 35 40 45 Phe Gln Pro Glu Pro Gln Ile Gly Glu Glu Asn Trp Gln Asp Ser Glu 50 55 60 Asn Tyr Tyr Gly Gly Arg Ala Leu Lys Pro Glu Thr Lys Met Lys Pro 65 70 75 80 Cys Tyr Gly Ser Phe Ala Arg Pro Thr Asn Ala Lys Gly Gly Gln Ala 85 90 95 Lys Ile Lys Pro Ala Gln Glu Gly Gln Gln Ser Ile Asp Tyr Asp Ile 100 105 110 Asp Leu Ala Phe Phe Asp Ile Pro Ser Thr Gly Gly Gly Asn Gly Thr 115 120 125 Asn Val Asn Asp Lys Pro Asp Met Val Met Tyr Thr Glu Asn Val Asn 130 135 140 Leu Glu Thr Pro Asp Thr His Leu Val Tyr Lys Pro Gly Thr Ser Asp 145 150 155 160 Asp Ser Ser Glu Ala Asn Leu Thr Gln Gln Ala Met Ala Asn Arg Pro 165 170 175 Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly Val Met Tyr Tyr Asn 180 185 190 Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln Ala Ser Gln Leu Asn 195 200 205 Ala Val Val Asp Leu Gln Asp Arg Asn Thr Glu Leu Ser Tyr Gln Leu 210 215 220 Leu Leu Asp Ser Leu Gly Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn 225 230 235 240 Gln Ala Val Asp Ser Tyr Asp Pro Asp Val Arg Ile Ile Glu Asn His 245 250 255 Gly Val Glu Asp Glu Leu Pro Asn Tyr Cys Phe Pro Leu Asp Gly Ala 260 265 270 Gly Thr Asn Ala Val Tyr Gln Gly Val Lys Thr Lys Glu Asp Asn Asn 275 280 285 Gly Glu Trp Glu Thr Asp Thr Asn Val Ala Ser Gln 290 295 300 <210> 2 <211> 940 <212> PRT <213> Artificial Sequence <220> <223> BLY6 hexon <400> 2 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Thr Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Tyr Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Ser Ser Gln Trp Gln Gln Lys Glu Asn Asn Gly Gln Gly 130 135 140 Asp Ala Lys Thr His Thr Tyr Gly Val Ala Ala Thr Gly Gly Ile Asp 145 150 155 160 Ile Asp Lys Asn Gly Leu Gln Ile Gly Ile Asp Glu Thr Lys Glu Asp 165 170 175 Asn Asn Glu Ile Tyr Ala Asp Lys Thr Phe Gln Pro Glu Pro Gln Ile 180 185 190 Gly Glu Glu Asn Trp Gln Asp Ser Glu Asn Tyr Tyr Gly Gly Arg Ala 195 200 205 Leu Lys Pro Glu Thr Lys Met Lys Pro Cys Tyr Gly Ser Phe Ala Arg 210 215 220 Pro Thr Asn Ala Lys Gly Gly Gln Ala Lys Ile Lys Pro Ala Gln Glu 225 230 235 240 Gly Gln Gln Ser Ile Asp Tyr Asp Ile Asp Leu Ala Phe Phe Asp Ile 245 250 255 Pro Ser Thr Gly Gly Gly Asn Gly Thr Asn Val Asn Asp Lys Pro Asp 260 265 270 Met Val Met Tyr Thr Glu Asn Val Asn Leu Glu Thr Pro Asp Thr His 275 280 285 Leu Val Tyr Lys Pro Gly Thr Ser Asp Asp Ser Ser Glu Ala Asn Leu 290 295 300 Thr Gln Gln Ala Met Ala Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp 305 310 315 320 Asn Phe Ile Gly Val Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val 325 330 335 Leu Ala Gly Gln Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp 340 345 350 Arg Asn Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp 355 360 365 Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp 370 375 380 Pro Asp Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro 385 390 395 400 Asn Tyr Cys Phe Pro Leu Asp Gly Ala Gly Thr Asn Ala Val Tyr Gln 405 410 415 Gly Val Lys Thr Lys Glu Asp Asn Asn Gly Glu Trp Glu Thr Asp Thr 420 425 430 Asn Val Ala Ser Gln Asn Gln Ile Cys Lys Gly Asn Ile Tyr Ala Met 435 440 445 Glu Ile Asn Leu Gln Ala Asn Leu Trp Lys Ser Phe Leu Tyr Ser Asn 450 455 460 Val Ala Leu Tyr Leu Pro Asp Ser Tyr Lys Tyr Thr Pro Ser Asn Val 465 470 475 480 Thr Leu Pro Thr Asn Thr Asn Thr Tyr Asp Tyr Met Asn Gly Arg Val 485 490 495 Val Ser Pro Ser Leu Val Asp Ala Tyr Ile Asn Ile Gly Ala Arg Trp 500 505 510 Ser Leu Asp Ala Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn 515 520 525 Ala Gly Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val 530 535 540 Pro Phe His Ile Gln Val Pro Gln Lys Phe Phe Ala Ile Lys Asn Leu 545 550 555 560 Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp 565 570 575 Val Asn Met Ile Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp 580 585 590 Gly Ala Thr Ile Gln Tyr Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe 595 600 605 Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn 610 615 620 Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met 625 630 635 640 Leu Tyr Pro Ile Pro Ala Asn Ala Thr Asn Val Pro Ile Ser Ile Pro 645 650 655 Ser Arg Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys 660 665 670 Thr Lys Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val 675 680 685 Tyr Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His 690 695 700 Thr Phe Lys Lys Val Ser Ile Met Phe Asp Ser Ser Val Ser Trp Pro 705 710 715 720 Gly Asn Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr 725 730 735 Val Asp Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp 740 745 750 Trp Phe Leu Val Gln Met Leu Ser His Tyr Asn Ile Gly Tyr Gln Gly 755 760 765 Phe Tyr Ile Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg 770 775 780 Asn Phe Gln Pro Met Ser Arg Gln Val Val Asp Gln Val Asn Tyr Lys 785 790 795 800 Asp Tyr Met Ala Val Thr Leu Ala Tyr Gln His Asn Asn Ser Gly Phe 805 810 815 Val Gly Tyr Leu Ala Pro Thr Met Arg Gln Gly Gln Pro Tyr Pro Ala 820 825 830 Asn Tyr Pro Tyr Pro Leu Ile Gly Lys Thr Ala Val Asn Ser Val Thr 835 840 845 Gln Lys Lys Phe Leu Cys Asp Arg Val Met Trp Arg Ile Pro Phe Ser 850 855 860 Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met 865 870 875 880 Leu Tyr Ala Asn Ser Ala His Ala Leu Asp Met Asn Phe Glu Val Asp 885 890 895 Pro Met Asp Glu Ser Thr Leu Leu Tyr Val Val Phe Glu Val Phe Asp 900 905 910 Val Val Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr 915 920 925 Leu Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 930 935 940 <210> 3 <211> 442 <212> PRT <213> Artificial Sequence <220> <223> BLY6 fiber <400> 3 Met Ser Thr Lys Arg Ala Arg Val Glu Asp Asp Phe Asp Pro Val Tyr 1 5 10 15 Pro Tyr Asp Ala Glu Leu Ala Pro Ser Val Pro Phe Ile Ala Pro Pro 20 25 30 Phe Val Ser Ser Asp Gly Phe Gln Glu Lys Pro Leu Gly Val Leu Ser 35 40 45 Leu Arg Leu Ala Asn Pro Val Thr Thr Lys Asn Gly Glu Leu Thr Leu 50 55 60 Lys Leu Gly Asp Gly Val Gly Ile Asp Ser Asp Gly Asn Leu Thr Ala 65 70 75 80 Gln Thr Val Thr Lys Ala Thr Ser Pro Leu Thr Val Ser Asn Asn Ala 85 90 95 Ile Ala Leu Asn Met Asp Lys Pro Phe Tyr Ser Ser Asn Gly Lys Leu 100 105 110 Ser Leu Gln Val Thr Ser Pro Leu Lys Ile Val Asp Ser Leu Asn Thr 115 120 125 Leu Ala Ile Gly Tyr Gly Gln Gly Leu Gly Leu Asn Asn Ser Ala Leu 130 135 140 Ala Val Gln Leu Ala Ser Pro Leu Thr Phe Asp Ser Asn Ser Lys Ile 145 150 155 160 Lys Ile Asn Leu Gly Ser Gly Pro Leu Lys Ile Asn Ala Asn Lys Leu 165 170 175 Ser Ile Asn Cys Leu Arg Gly Val Tyr Val Thr Thr Asp Gly Thr Ser 180 185 190 Ile Glu Thr Asn Ile Ser Trp Ala Lys Gly Met Arg Phe Glu Gly Asn 195 200 205 Ala Met Ala Val Asn Val Asp Ser Thr Lys Gly Leu Gln Phe Gly Thr 210 215 220 Thr Ser Thr Glu Ser Gly Val Thr Asn Ala Phe Pro Ile Gln Leu Lys 225 230 235 240 Ile Gly Ser Gly Leu Ser Phe Asp Ser Thr Gly Ala Leu Val Ala Trp 245 250 255 Asp Lys Asp Asn Asp Lys Leu Thr Leu Trp Thr Thr Ala Asp Pro Ser 260 265 270 Pro Asn Cys Thr Ile Tyr Thr Asp Lys Asp Ala Lys Leu Thr Leu Cys 275 280 285 Leu Thr Lys Cys Gly Ser Gln Ile Leu Gly Ser Val Ser Val Leu Ala 290 295 300 Val Lys Ala Gly Thr Leu Gln Pro Ile Ser Glu Lys Ile Gly Thr Ala 305 310 315 320 Leu Val Ser Leu Lys Phe Asn Asn Asn Gly Val Leu Leu Ser Asn Ser 325 330 335 Thr Leu Ser Asn Glu Tyr Trp Asn Tyr Arg Lys Gly Asp Val Thr Pro 340 345 350 Ala Glu Ala Tyr Thr Asn Ala Val Gly Phe Met Pro Asn Ile Lys Ala 355 360 365 Tyr Pro Lys Asn Thr Asn Ser Ala Ser Lys Ser His Ile Val Gly Gln 370 375 380 Val Tyr Leu Asn Gly Asp Glu Thr Lys Pro Met His Leu Ile Ile Thr 385 390 395 400 Phe Asn Glu Thr Ser Asp Glu Thr Cys Thr Tyr Ser Ile Thr Phe Gln 405 410 415 Trp Lys Trp Asn Ile Gly Thr Tyr Thr Ser Asp Thr Leu Ala Thr Ser 420 425 430 Ser Phe Thr Phe Ser Tyr Ile Ala Gln Glu 435 440 <210> 4 <211> 938 <212> PRT <213> Artificial Sequence <220> <223> SAdV-30-1 hexon <400> 4 Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala 1 5 10 15 Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala 20 25 30 Arg Ala Thr Asp Thr Tyr Phe Ser Leu Gly Asn Lys Phe Arg Asn Pro 35 40 45 Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu 50 55 60 Thr Leu Arg Phe Val Pro Val Asp Arg Glu Asp Asn Thr Tyr Ser Tyr 65 70 75 80 Lys Val Arg Tyr Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met 85 90 95 Ala Ser Thr Tyr Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Ser 100 105 110 Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly 115 120 125 Ala Pro Asn Ser Ser Gln Trp Glu Gln Asn Glu Asn Asn Gly Gln Gly 130 135 140 Gln Ala Lys Thr His Thr Tyr Gly Val Ala Ala Met Gly Gly Leu Asp 145 150 155 160 Ile Thr Lys Glu Gly Leu Gln Ile Gly Thr Asp Ala Ser Lys Glu Asp 165 170 175 Asp Asn Glu Ile Tyr Ala Asp Glu Thr Tyr Gln Pro Glu Pro Gln Ile 180 185 190 Gly Glu Glu Asn Trp Gln Asp Thr Glu Asn Phe Tyr Gly Gly Arg Ala 195 200 205 Leu Lys Lys Asp Thr Lys Met Lys Pro Cys Tyr Gly Ser Phe Ala Arg 210 215 220 Pro Thr Asn Val Lys Gly Gly Gln Ala Lys Val Lys Thr Glu Glu Asn 225 230 235 240 Val Gln Ser Phe Asp Ile Asp Leu Ala Phe Phe Asp Ile Pro Ser Thr 245 250 255 Gly Thr Gly Ser Asn Gly Thr Asn Val Asn Asp Lys Pro Asp Met Val 260 265 270 Met Tyr Thr Glu Asn Val Asn Leu Glu Thr Pro Asp Thr His Ile Val 275 280 285 Tyr Lys Pro Gly Thr Ser Asp Asp Ser Ser Glu Ala Asn Leu Cys Gln 290 295 300 Gln Ala Met Pro Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp Asn Phe 305 310 315 320 Ile Gly Leu Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala 325 330 335 Gly Gln Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn 340 345 350 Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr 355 360 365 Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp 370 375 380 Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn Tyr 385 390 395 400 Cys Phe Pro Leu Asp Gly Ala Gly Thr Asn Ala Val Tyr Gln Gly Val 405 410 415 Lys Glu Lys Thr Gly Asn Asn Gly Glu Trp Glu Ala Asp Thr Asn Val 420 425 430 Ala Ser Gln Asn Gln Ile Cys Lys Gly Asn Ile Tyr Ala Met Glu Ile 435 440 445 Asn Leu Gln Ala Asn Leu Trp Arg Ser Phe Leu Tyr Ser Asn Val Ala 450 455 460 Leu Tyr Leu Pro Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Ile Thr Leu 465 470 475 480 Pro Thr Asn Thr Asn Thr Tyr Asp Tyr Met Asn Gly Arg Val Val Pro 485 490 495 Pro Ser Leu Val Asp Ala Tyr Ile Asn Ile Gly Ala Arg Trp Ser Leu 500 505 510 Asp Pro Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala Gly 515 520 525 Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro Phe 530 535 540 His Ile Gln Val Pro Gln Lys Phe Phe Ala Ile Lys Ser Leu Leu Leu 545 550 555 560 Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val Asn 565 570 575 Met Ile Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp Gly Ala 580 585 590 Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe Pro Met 595 600 605 Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn Asp Thr 610 615 620 Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met Leu Tyr 625 630 635 640 Pro Ile Pro Ala Asn Ala Thr Asn Val Pro Ile Ser Ile Pro Ser Arg 645 650 655 Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys Thr Lys 660 665 670 Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val Tyr Ser 675 680 685 Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His Thr Phe 690 695 700 Lys Lys Val Ser Ile Thr Phe Asp Ser Ser Val Ser Trp Pro Gly Asn 705 710 715 720 Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp 725 730 735 Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe 740 745 750 Leu Val Gln Met Leu Ala His Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr 755 760 765 Val Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn Phe 770 775 780 Gln Pro Met Ser Arg Gln Val Val Asp Glu Val Asn Tyr Lys Asp Tyr 785 790 795 800 Gln Ala Val Thr Leu Ala Tyr Gln His Asn Asn Ser Gly Phe Val Gly 805 810 815 Tyr Leu Ala Pro Thr Met Arg Gln Gly Gln Pro Tyr Pro Ala Asn Tyr 820 825 830 Pro Tyr Pro Leu Ile Gly Lys Ser Ala Val Thr Ser Val Thr Gln Lys 835 840 845 Lys Phe Leu Cys Asp Arg Val Met Trp Arg Ile Pro Phe Ser Ser Asn 850 855 860 Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met Leu Tyr 865 870 875 880 Ala Asn Ser Ala His Ala Leu Asp Met Asn Phe Glu Val Asp Pro Met 885 890 895 Asp Glu Ser Thr Leu Leu Tyr Val Val Phe Glu Val Phe Asp Val Val 900 905 910 Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr Leu Arg 915 920 925 Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr 930 935 <210> 5 <211> 35873 <212> DNA <213> Artificial Sequence <220> <223> BLY6 genome <400> 5 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgtaactgtc agctgatccc tagtgtattt 480 aaacctgtgc agttccgtca agaggccact cttgagtgcc agcgagaaga gttttctcct 540 ccgcagcgcg agtcaaatct acacttctaa gatgagacac cgagtgattc ctgaggagaa 600 attctctatt agcgccggac tggaaattct acagatggtg gtggatattc taacagaggg 660 agaaccggag gctgctaact ttaactacga tcctccttcg ctccacgatc tgtatgattt 720 agaggtaaat gaaccagaga ctgaggcatt tgtgaatgcc gcgtttcctg attctctcct 780 gagtaaagct gaggagagtg agggttccag cgagcctgca actccaagtc ctccgagacc 840 tggaataggc gaaaaaagat tgcctggatg tcataaatcg cctgttgggg aaatggactt 900 gatgtgttat gaggacggct ttcctccgag cgatgaggag gaagatacac agcaggtgct 960 tgaagcctcc actagtcatg gaacggaggt gtttaatctc gactgcccag agctgcccgg 1020 ccatggctgt aagtcttgtg attatcatcg aaagagcact ggcttggagg gattgttatg 1080 ctcgctttgc tatatgcgcc taaacagcca ttatattttc agtaagtttt tttaattttg 1140 gtttaaaggg acagcgcagc gtgtctgatt gtattaagat ttggaaactt aatgtttgtg 1200 tatgtgtttc ttatataggc cctgtgtctg actcagatga gacccccacc ccagactcca 1260 ctacctcacc cccggaaatt cagatgcata ttccagagaa tgtgtgtaag ccaattcctt 1320 tgagacccac gcctggaaaa cgtcatgctg ttgaaagact tgaggactta ttgatgggtg 1380 gggatgaacc tttggacttg agcttgccta aacagccaag gcaataaaat gtccacacct 1440 gtgtttgctc aatgatgtca tgcaataaat tatgtcacat accaaaggtg tgttttatga 1500 ctaggggtgg ggctactggg tatataagta ggagcagact gttgtagtta gctgagagca 1560 gcgagctgtc tctatggagg tttgggctgc attggaagat ctccataaca ctagatccct 1620 gctagaaaac gcctcggacg gtgtctctag aatttggaga tactggtttg gtggcgagct 1680 agctagtgtg atctataggg ttaagcagga atataagggc gaatttgaag aacttttact 1740 ggactgtcct ggtcttttgg agtctttgaa tttaggtcat cagatccagt ttaacgagaa 1800 gattttatca gctttagatt tttcaacacc aggcagatct gcagcttctg ttgcttttat 1860 agttcacatt ttagacagat ggagtcagga aactcaccta agttgggggt acctcttgga 1920 tttcttagcg acagcgttgt ggagacaatg gaagagggtc agggcgagag taacctcaga 1980 ttattggccg gtgcagccgc tgggtgtagc acatctgctg agacacacca tggaactacc 2040 aacaattccg gaggaaccag ccctggagga gcaggaagag aacccgagag ccggcctgga 2100 ccctccaacg gaggaggagg aataagtgac ttgttccctg aactgcgacg ggtgcttact 2160 aggagtacat ctggaagggg ggataggggc gtgaagcgag aaaggaagga gtctaatcct 2220 aaagtggatc ttacagttag cttgatgaat cgcaaccgtc cagaggttat tacatggact 2280 gaggtgcaac aggaatgcag ggatgaattt tcccttttac aggagaaata ctcattagaa 2340 caggtgaaga catattggct ggagccagaa gatgattggg aggcagtgat tagaaattat 2400 gctaaggtgg ctcttagacc agataaaaag tatatagtaa ctaaaaagat ctgtgttagg 2460 aatgcttgct acattgtggg taatggggca gaggttcaga taaacaccac agatagggtg 2520 gcttttagat gttgtatgat gggcatgaga cctggggtga gtgggatgga ggctgtgaca 2580 tttatgaata taaaattcag aggagatggg tttaatggca cagtgtttat ggccaatact 2640 aaattaattc tgcatgggtg cagtatgttt gggtttaata atacttgtgt ggaagcctgg 2700 ggggaggtga gcataagggg gtgcagtatc tttggttgtt ggacgggagt ggttggtagg 2760 acaaagagca tggtatctgt gaagaaatgt atgtttgaaa ggtgcacact gggtatcctg 2820 agcgagggag aagctaggat tcgcaactgt gctttcacag atagtgggtg tggcatactt 2880 gtgaagggaa atgccagtat aaagcataat atgttctgcg gaccatccga tgatcgatca 2940 taccagatgg tgacctgtgc cagtggcaat tgcgatttgc tggctactat tcacattgtg 3000 acccatcaac gcaaaagatg gcctgtgttt gagcataatg tactgaccag gtgtaacgtt 3060 catctgggtg gtcgtagagg aatgttcatg ccataccaat gcaattttaa tcatgtgagg 3120 atcttgatgg agccgcaagc gttttccaga gtcagcttga ctggaatctt tgacatgtgt 3180 gtggaagcat ggaagatctt aagatatgat gataccaaat ccagatgccg cgcatgcgag 3240 tgcgggggca ggcatgccag gttccaacct gtatgtgtgg aggtgaccga ggagctgaga 3300 ccagatcatt tggtgctgac ctgcactggt gcggagttcg gttccagtgg tgaagaaact 3360 gattaaagtg agtagtggga tgttataaaa gtgaccataa ggtgatgtga gatggacaaa 3420 tttggtaatt tttatgtatt tttgtcttgc agccatgagt gggagcgctt cctttgaagg 3480 gggcgtcttt agcccttatc tgacggggcg tctgcctcat tgggctggag tgcgtcagaa 3540 tgtgatgggg tctacagtgg atggaagacc tgttcagcct gctaattctt ctactctgac 3600 ttatgctact atgacttcct cgcctttgga tgcagctgca gctgctgccg cttctgctgc 3660 cgccaacact gttcggggga tggccttgga gatggggtat tatggaactg tagtggccaa 3720 caccactacc ccaaataacc ccacagcctt gaatgaggac aagctgctag ttctcatgtc 3780 ccagctggag tctttgaccc aacgcctggg cgatctagct cagcaggtgt cccagctgaa 3840 ggagcagact caagctgcaa ttaccactgc gaggggaaat taaaaaaatt caaagaatca 3900 ataaataaac cgagactttg ttgattttaa agtgtgtcat tctttattta atttttcgcg 3960 cgcgatatgc cctggaccac cggtctctat cattgaggac acggtggatc ttttctagaa 4020 cccgatagag gtgggattgg atgttgaggt acatgggcat aagaccatct ttggggtgta 4080 gatagctcca ctgcagagcc tcatgctccg gggtggtgtt gtatataacc cagtcatagc 4140 atgggcgttg ggcatgatgt tgcacaatat ctttaaggag gagactaatg gccactggga 4200 gacccttggt gtaagtgttt acaaatctat taagctggga cgggtgcatc cgaggtgaga 4260 taatgtgcat tttggattgg atttttagat tggcaatgtt tccccctaga tctctcctgg 4320 gattcatgtt atgcaagacc actagaacag tgtatccggt gcacttaggg aatttgtcat 4380 gaagtttgga ggggaaagca tgaaaaaatt tagacacacc cttgtgtcct cccaagttct 4440 ccatgcactc atccataata atggcaatgg gcccatgggc ggcggcacgg gcgaacacgt 4500 tcctgggatc tgacacatca tagttgtggt cttgggtcag gtcatcataa gccattttaa 4560 taaacttggg gcggagggtg ccagattggg ggatgaatgt tccctcgggc cccggaacat 4620 agtttccttc acatatttgc atttcccagg cttttagttc agaggggggg atcatgtcca 4680 cctgtggagc gatgaagaag acggtctcgg gggcgggggt gattaagtgg gaggacagca 4740 agttcctaag cagctgtgac ttgccacacc cagtgggacc gtagatgacc cctataacag 4800 gttgcagatg gtagtttagg gaaagacagc tgccgtcctc tcgcaggagg ggggcgacct 4860 cgttcatcat ttccctcaca tgcatgtttt cccgcacaag ttccgatagg aggcgctctc 4920 cacccaggga aaggagttct tgaagagatg agaaattttt caagggtttt aagccatcag 4980 ccatgggcat tttggagagg gtttgttgca agagttcaag gcggtcccag agttcggtga 5040 tgtgttctat ggcatctcga tccagcatac ttcctcgttt ctggggttgg gacggctgcg 5100 ggagtatgga accaggcgat gggcgtccag cgctgccagt gtccggtcct tccacggtcg 5160 cagcgtccga gtcagggtcg tttccgtcac ggtgaagggg tgcgcgcctg gctgggcgct 5220 tgcgagggtg cgcttcaggc tcatcctgct cgtggagaac cgctgccgtt ctgcgccctg 5280 tgcatcggcc aggtagcaat taaccatgag ttcgtagttg agcgcctctg ccgcgtggcc 5340 tttggcgcgc agcttacctt tggaagtctt ctgacaggtg ggacagtaga gacacttgag 5400 agcatagagt tttggggcta gaaagaccga ttctggggag tatgcatcgg ccccacagga 5460 ggcgcagacg gtttcgcatt ccaccagcca tgtaagatcg ggctcgttgg ggtcaaaaac 5520 aagttttccg ccatgttttt tgatgcgttt cttacctttg ctttccatga gttcgtgccc 5580 ccgttgggtg acaaagaggc tgtccgtgtc cccgtagact gactttatgg gcctgtcctc 5640 gagcggcgtg ccgcggtcct cttcgtagag gaactcggac cactctgaga cgaaagcacg 5700 tgtccaggcc agcacaaagg aggctatatg ggaggggtag cgatcgttgt caaccaaggg 5760 gtctactttt tccaaggtgt gtaaacacat gtccccttct tccacatcca ggaaggtgat 5820 tggcttgtaa gtgtatgcca cgtgacctgg ggtcccagac gggggggtat aaaagggggc 5880 gggtctctgc tcgtcctcac tgtcttccgg atcgctgtcc aggagcgcca gctgttgagg 5940 taggtattcc ctctcgaagg cgggcataac ctccgcactc aggttgtcag tttctaggaa 6000 cgaggaggat ttgatattga cagtgcctgc cgagatgcct ttcatgagac tgtcgtccat 6060 ttggtcagaa aagacaatct ttttgttatc aagtttggtg gcgaaggatc catacagggc 6120 attggaaagc agtttggcaa tggagcgcat ggtttggttt ttttctttgt ctgcgcgctc 6180 tttggcggct atgttgagtt ggacatattc gcgggccaga catttccatt gtggaaatat 6240 ggtagttaat tcatctggga cgattctgac tttccagcct ctgttatgca gggtaatcag 6300 atccacactg gttgccactt ctcctctaag tggttcatta gtccagcata gtcgcccccc 6360 ttttcgagaa cagaaagggg gtaggggatc tagcatgagt tcgtctgggg ggtctgcatc 6420 tatggtgaaa atcccaggaa ggagatcttc gtcaaaatag ctgatggtgg cggggtcatc 6480 cagagacatt tgccattctc gagcagccag agcgcgctcg taggggttaa ggggagtccc 6540 ccatggcatg ggatgggtga gtgcagaagc atacatgcca cagatgtcat agacatagag 6600 cggctcttcc agaatcccta tgtaagtggg ataacatcgc ccccctctga tgctggctcg 6660 cacataatca tagagttcat gtgagggcgc tagaagaccc gagcccaggt tggtgcggtt 6720 gggtttttct gctctgtaga ggatctggcg aaagatggca tgggagtttg atgagatggt 6780 gggtctttgg aagatgttga aatgggcatg aggcagtccc acagagtccc ttatgaagtg 6840 agcataggag tcttgcagtt tggccaccag ctcggcggtg accagcacat ccaaagcaca 6900 gtagtcgagg gtctctttga tgatgtcata gttaggttcc cctttctttt cccacagctc 6960 gcggttgaga aggtattctt cgcgatcctt ccagtactct tcgaggggga acccgtcctt 7020 gtctgaacgg taagaaccca gcatgtaaaa ttgattgaca gctttgtagg cacaacaccc 7080 cttctccacg gggagtgagt atgcttgcgc ggctttgcgc agagaggtgt gagtaagggc 7140 gaaagtgtcc ctgaccatga ctttgaggaa ctgatgctta aagtctatgt catcgcaggc 7200 cccctgctcc cacagttgga agtccactcg ctttttgtag gcgggattgg gcaaagcgaa 7260 agtaacatcg ttgaatagga tctttccagc cctgggcatg aagttgcgag taatgcgaaa 7320 aggctgaggc acttctgccc tgttgttgat aacttgggca gccaagacga tctcgtcaaa 7380 gccgttgatg ttgtgaccca caatgtacag ttctacgaag cgtgggcgtc ccttgatgtg 7440 gggcagtttt ttaagctctt cgtaggtcaa gtcgtcaggg tcagcgattc catattgctc 7500 caaagcccag tcaggcaggt gaggattagc atgaaggaaa gaggtccaaa gatccacggc 7560 cagagctgtt tgtaagcggt ctctgtactg acggaaatgt cggcctaccg ccattttttc 7620 aggagtaaca cagtaaaagg tgcgcgggtc cttttcccag cgatcccatt gaagttgcaa 7680 ggctaggtcg tgggcgaggt tgacgagctg ttcgtccccc gaaagtttca tgaccagcat 7740 gaaagggaca agctgcttgc caaaggaccc catccaggtg taggtttcca catcgtaggt 7800 gaggaagagc ctttctgtgc gaggatgaga accgatcggg aagaactgga tttcctgcca 7860 ccagttggag gaatggctgt tgatatgatg gaagtagaac tccctacggc gcgccgagca 7920 ttcgtgcttg tgcttgtaca gacggccaca gtactcgcag cgctgcacgg gatgcacctg 7980 atgaatgagc tgtacctggc ttcctttgac aagaaatttc agtgggaagt tgaggcgtgg 8040 cgtctgcatc tcgtgttgta ttacgtcctg gctattggtc tggccatctt ctgtctcgat 8100 ggtggtcatg ctgacgagcc cgcgcgggag gcaggtccag acctccgcgc ggacgggtct 8160 gagagcgagg acgagagcgc gcaggccgga actgtccagg gtcctgagac gctgcggagt 8220 caggtcagta gggagagtac ataggtttac ttgcataagt ttttccaggg catgtgggag 8280 gtcaagatga tatttgattt ctactggcga gttggtggag acatcgatgg cttgcagggt 8340 cccgtgcccc tggggtgcta ccaccgtccc tttttttttc ttgatcgggg gcggtgttgc 8400 ttcttgcatg gtaaggtcgt cttctagaag cggcggcgag gtcgcgcgcc gggtggcagt 8460 ggcggttctg gacctggagg tagaggcggt agaggtacgt cggcgccgcg cgcgggtagg 8520 ttctggtact gcgccctgag aagacttgcg tgagcgacga cgcggcggtt gacgtcctgg 8580 atctgacgcc tctgggtgaa tgctaccgga cccgtgagct tgaacctgaa agagagttca 8640 acagaatcaa tttcggtatc gttgacggct gcctgccgca ggatttcttg tacgtcgccc 8700 gagttgtctt ggtaggcgat ctcggccatg aactgctcga tctcttcttc ttggagatct 8760 ccgcggcccg ctcgttctac ggtggcagca aggtcgttgg agatgcgccc catgagctgt 8820 gagaatgcat tcatgcccgc ctcgttccag acgcgactgt agaccacggc tccctcggga 8880 tctctggcgc gcatgaccac ttgggcgagg tttagttcca cgtgtctggt gaagaccgca 8940 tagttgcaga gacgctggaa gaggtagttg agcgtggtgg cgatgtgctc ggtgacaaag 9000 aaatacatga tccagcgacg aagcggcatc tcgctgatat cgcccagggc ttccaaccgt 9060 tccatggctt cgtaaaagtc cacggcgaag ttgaaaaact gggagttgcg agcggacacg 9120 gtcaactcct cctccagaag acggatgagc tcggcgatgg tggcgcgcac ttcgcgctca 9180 aaggctcccg ggatctcttc ctcctcttct tcttccaact cttcctccac taacatctct 9240 tctacttcct cctcaggcgg cgggggtgga ggagggggcg cgcggcgacg ccggcgacgc 9300 acgggcagac gatcgatgaa gcgttcgatc acttctccgc ggcggcgacg catggtctcg 9360 gtgacggcgc gcccgtcctc cctgggtcgc agagtgaaga cgccgccgcg cagctccctg 9420 aaatggtgac tgggagggtc cccgtttggt agggacaggg cactgatgat gcatcttatt 9480 aattgccctg tagggactcc gcgcaaggac ctgagcgtct cgagatccac gggatctgaa 9540 aatctctgaa cgaaggcttc gagccagtcg cagtcgcaag gtaggctgag cactgtttct 9600 tcggggcggg ctgctgagct agagggttgt acgatgctgc tggtgatgaa gttaaaatag 9660 gcagttctga gacggcggat ggtggcgagg agcaccaggt ctttgggtcc ggcttgctgg 9720 atgcgcaggc ggtcggccat tccccatgca ttatcttggc acctggccag atctttatag 9780 tagtcttgca tgagtcgctc cacgggcact tcttcttcgc ccgctctgcc gtgcatgcgt 9840 gtgagtccgt accctctctg tggttggacg agcgccaggt cggcaacgac cctttcggct 9900 agaatggctt gctgcacctg ggtgagggtg ttctggaaat catcaaagtc cacaaagcgg 9960 tggtaggccc ccgtgttgat ggtgtaggag cagttggaca tgaccgacca gttgactgtc 10020 tggtgtcctg gtcgtacgag ttccgtgtac ctgagccgcg agtatgcgcg ggagtcgaag 10080 atgtaatcgt tgcaggttcg caccaggtac tggtagccga tgaggaagtg aggcggcggc 10140 tggcggtaga gaggccatcg ttcggtggcg ggcgcgccgg gcgctaggtc ttctagcatg 10200 agacggtggt atccgtagac gtacctggac atccaggtaa taccggcggc ggtggtggag 10260 gcgcgcggaa actctcgcac gcggttccag atgttgcgca gcggcatgaa gtagttcatg 10320 gtgggcacgg tctggcccgt gaggcgcgcg cagtcattga tgctctagat acgggcaaaa 10380 acgaaagcgt tgagcggttc ccttccgtgg cctggaggaa cgcgaacggg ttaggtcgca 10440 gcgtaccctg gttcgagact aaagaaagcg agcaactcga accggcagag tcgcggctaa 10500 cgggtattgg caatcccgtc tcgacccaag ccagcaaatc caggatacgg atgggggccc 10560 cttttgtttt tcagggcatg agtcaccggt taaggtttac aacggctgtt tcatgccttt 10620 agaagtggct cgcgcccgta gtctggagaa tcaatcgcca gggttgcgtt gcggcgtgcc 10680 ccggttcgag cctgcagctt gagtcggccg gtgaccgcgg caaacgaggg cgtggcggcc 10740 ccgtcgtttc taagaccttg ctagccgacc tctccagttt acgggaacga gccccctttt 10800 attttttttg tttttgccag atgcatcccg tactgcggca gatgcgccca cagcccccac 10860 agcagcagca gcaggctggc ctaccttctc tacctcagcc gctacctgca actaccgcgg 10920 tggccgctgt aagcggggcc ggacagcagg cggctcctca atatgaattg gacttggaag 10980 agggcgaggg attggcaaga ttgggggcgc cctcgcccga gcgccacccg cgggtgcaga 11040 tgaaaaagga cgttcgcgaa tcttacgtgc ccaagcagaa tctgttcaga gacagaagcg 11100 gcgaggagcc cgaggagatg cgcgcgtccc gttttaacgc gggtcgcgag ctgcgacaag 11160 gactggatcg aaaacgggtg ttgagggatg atgattttga ggtggatgaa atgacaggga 11220 tcagccccgc tcgcgctcac gtggctgcag ctaatctggt gacagcttat gagcagaccg 11280 tgaaggagga aagcaacttc cagaaatcat tcaataacca cgtgcgcacc ctgatcgcac 11340 gcgaggaggt gaccctgggc ctgatgcacc tgtgggatct gctggaagcc atagtgcaga 11400 accccactag caaacccctg actgctcaac tgtttctggt ggtgcagcac agcagggata 11460 atgaggcatt cagagaggcg ctgctgaata tcactgaacc tgaggggaga tggctgctgg 11520 atctggtgaa tatcctgcag agcattgtag tgcaggaacg cagcttgcct ttgtccgaga 11580 aggtggcggc gatcaattac tctgtgctga gtctgggcaa atactatgcc aggaagatct 11640 acaaaacccc ttacgtgccc atagacaagg aagtgaaaat agatgggttt tacatgcgca 11700 tgaccctgaa agtgctaacc ctgagcgatg acttgggagt gtaccgcaac gacaggatgc 11760 accgcgcggt gagcgccagc aggaggcgcg agctgagcga caaagaatta atgcacagct 11820 tgcaacgagc cctgacggga gccgggacgg agggggagaa ctactttgac atgggtgcag 11880 acttgcattg gctgcctagt cgcagggcat tggaagcggc aggcgatggg ccctatgtag 11940 aggaagtagt agacgaggac gatgaggagg gcgagtacct ggaagactga tggcgcgacc 12000 cgtatttttg ctagatggaa caggcgccgg accctgcgat gcgggcggcg ctgcagagcc 12060 agccgtccgg cattaattcc tcggacgatt ggacccaggc catgcaacgc atcatggcgc 12120 tgacgacccg caaccccgaa gcctttagac agcagcctca ggccaaccgc ctttcggcca 12180 tcctggaggc cgtggtgccc tctcgctcca accccaccca cgagaaggtt ctggccatcg 12240 tgaatgccct ggtggagaac aaggccatcc gctccgatga agccgggctg gtatacaacg 12300 ccttgctcga gcgcgtggct cgctacaaca gcagcaatgt ccagactaac ctggacagga 12360 tggtgaccga cgtgcgcgag gccgtgtccc agcgcgaacg gttccatcgc gagtctaacc 12420 tgggttccat ggtagcgctg aacgctttcc tcagttccca gcctgccaat gtgccccggg 12480 gacaggaaga ctataccaac tttattagcg ccctgagact catggtagcc gaggttcctc 12540 agagcgaggt gtaccagtcc ggtccagact actttttcca gacaagcagg aacggtatgc 12600 agacagtgaa cttaagccag gctttcaaga acctgcaagg gctgtgggga gtccaagctc 12660 cagtgggcga cagggcgacc gtgtcgagcc tgttgactcc aaattcccgt ttgctgctgc 12720 tgctggtgtc ccccttcact gacagcggca gcataaacag aaactcctac ttgggctacc 12780 tgataaactt gtatcgcgaa gctataggtc aggcccacgt ggacgaacag acctatcagg 12840 agatcactaa tgtgagtcgc gctctgggcc aggacgaccc tggaaacctg gaagctactc 12900 taaactttct gctgaccaac cgctcgcaaa aaatccctcc tcagtataca ttaactgcgg 12960 aggaggaacg gatcttgaga tacgtgcagc agagcgtggg tctgttcctg atgcaagagg 13020 gtgcgacccc tagcgccgcg cttgatatga cagcgcgcaa catggagccc agcatgtatg 13080 ccagcaacag accattcatt aataaattga tggattactt ccatcgcgcg gccgctatga 13140 actctgatta cttcaccaat gctattctga acccccattg gctgcctccg cctggttttt 13200 atactggcga gtatgacatg cctgacccca acgatgggtt cttgtgggac gatgtggaca 13260 gcgtggcgtt ctcgcctacc gctcctcgta ctttttggaa gaaggaaggt agtgacagaa 13320 gaccctcctc cgtgctgtca ggacgtgagg gtgctgccgc ggcggtcccc gatgctgcaa 13380 gcccctttcc cagtctgcca ttttcactaa acagcgtgcg cagtagcgag ctggggagaa 13440 taacccgccc tcgcttgctg ggcgaggacg agtatttgaa tgactcccta ctgagacccg 13500 agcgggaaaa gaacttccct aataatggga ttgaaagcct ggtggataag atgagcagat 13560 ggaagaccta tgcccaggag cacagagatg agcctagaat cttgggtcct acagtaggca 13620 cccgcagacg ccagcgccat gatagacagc ggggtctggt gtgggacgat gaggattctg 13680 cagatgacag cagcgtgttg gacttgggcg ggaggggagg tgtgggcaac ccgttcgcac 13740 acttgcgtcc ccgtattgga cgcatgatgt aaaagtgaaa ataaaaaagg aactcaccaa 13800 ggccatggcg accagcgtgc gttcgttctt tctgttgttg tatctagtat gatgaggcgc 13860 accgtgctag gcggatcggt ggcgtatccg gagggtcctc ctccttcgta cgaaagcgtg 13920 atgcagcagg tggcggcggc ggcgatgcaa ccccccttgg aggctcctta cgtgcccccg 13980 cggtacctgg cacctaccga ggggagaaac agcattcgtt attcggaact cacacccttg 14040 tatgacacca cccggttgta cctggtggac aacaaatcgg cggacattgc ctcgttgaac 14100 tatcagaacg accacagcaa cttcttgaca acggtggtgc agaacaatga ctttaccccc 14160 acggaggcca gcacccagac catcaacttt gacgagcgct cccggtgggg cggtcagctg 14220 aagaccatca tgcacaccaa catgcccaac gtgaacgagt tcatgtttag caacaagttc 14280 agggctaggg tgatggtgtc cagaaccaca cctaaagagg tgacagtcac aacagactat 14340 gatggtagtc aggacatctt ggaatacgag tgggttgact ttgagttacc agaaggcaac 14400 ttctctgcca ccatgaccat agacctgatg aataatgcaa ttgttgataa ttacctaaaa 14460 gtgggtagac agaatggggt actggagagt gacataggtg ttaagtttga cactaggaac 14520 tttaggcttg gttgggaccc agtgacagag ttggtcatgc ctggggtcta caccaatgaa 14580 gctttccatc ctgacatagt cctactacct ggctgcggag tggacttcac tgagagccgc 14640 ctcagtaatc tgctaggcat tagaaagaaa cagccattcc aggaagggtt ccagatcatg 14700 tatgaggatc tggagggtgg taacatcccc gccctgcttg atgtaaatgc atatgagaag 14760 agcaaggaag ataatacaac caccacaaat gaagctgtgg ccgcggcttc atctactgaa 14820 gccaaagctg tggtagatgc ttccacttca acagaaaaca ccactgatga aaaagtcacc 14880 aggggagata catttgccac ccctgaacaa gagaaggcag ctgaggcaga gtctgatatt 14940 atgcttctgt ccaccgatga aaacgaaact aaaaaacaac tggttattcg agcggtgacc 15000 aaggatagta aggacaggag ttataatgta ttgtcagatg gaaagaacac agcttaccgt 15060 agttggtacc tggcatacaa ttatggcgac cgtgagaaag gggtgcgttc ttggacactg 15120 cttaccacct cggatgtcac ctgcggcgtg gagcaagtct attggtcgct accagatatg 15180 atgcaagatc cagtcacctt tcgctccaca cgccaagtta gcaactaccc agtggtgggc 15240 gcagagctgc tcccagtgca ttccagaagc ttctacaacg agcaagccgt ctactcgcaa 15300 cagctccgcc agtacacctc gctcacgcac gtcttcaacc gcttccccga gaatcagatc 15360 ctcgtccgcc cgcccgcgcc aaccattacc accgtcagtg aaaacgttcc tgctctcaca 15420 gatcacggga ccctgccgct gcgcagcagt atccggggag tccagcgcgt gaccgttact 15480 gacgccagac gccgcacctg tccatacgta tacaaggccc tgggcatagt cgcgccgcgc 15540 gtcctttcaa gccgcacttt ctaaaaaaat gtccattctc atctcgccca gtaataacac 15600 cggttggggc ctgcgcacac ctagcaagat gtatggaggc gctcgcagac gctccactca 15660 gcaccctgtg cgcgtgcgcg ggcatttccg cgctccctgg ggcgccctca agggacgctc 15720 tcgtactagg accaccgttg acgatgtgat cgaccaggtg gtcgccgatg cacgtaacta 15780 tacccccgca gccgcacctg catccaccgt ggatgcggtc attgacagcg tggtagccga 15840 tgcgcgcgcc tatgctcgcg ccaagagcag gaggcggcgt attgccaggc gtcaccgagc 15900 tactccagcc atgcgagctg caagagcttt attgcggaga gccagacgtg tggggcgaag 15960 agccatgcgt agagcggcca gacgcgcggc ttcaggtgcc agcgcaggca gggtccgcag 16020 gcgcgcggct acggcggcag cggcggccat cgctagcatg accaaaccac gaagaggcaa 16080 tgtgtattgg gtgcgcgacg ccgccaccgg ccagcgcgtg cccgtgcgca cacgcccccc 16140 tcgcacttag aagatactga gcagtctccg atgttgtgtc ccagcggcga gatgtccaag 16200 cgcaaattca aggaagagat gctccaggtc atcgcgcctg agatctacgg tcctgcggtg 16260 aaggatgaaa aaaagccccg caagatcaag cgggtcaaaa aggacaaaaa ggaagaagat 16320 ggtgatgatg ggctggtgga gtttgtgcgc gagtttgccc caaggaggcg cgtgcagtgg 16380 cgcgggcgca aagtgtggcc ggtgttgaga ccggggacca cagtggtctt tacgccaggc 16440 gagcgctcca gcaccgtttc caaacgctct tatgatgagg tgtacgggga cgatgatatt 16500 ctcgagcagg cggctgatcg ccttggcgag tttgcatatg gcaaacgcag ccgctcggga 16560 gccaaggaag aggcattgac catccccttg gatcatggaa atcccacccc aagcctcaaa 16620 cccgtgaccc tgcaacaagt gctgcccacg ccgccacgca agggcatcaa gcgcgagggc 16680 gaggatctgt atcccaccat gcagctgatg gtgcccaagc gccagaagct ggaagacgtg 16740 ctggagaaaa tgaaagtgga tcctgaaatc cagcctgaag tcaaagtgag gccaatcaag 16800 caggtggcgc ccggtttggg ggtacaaacc gtggatatca agatccccac cgagtccatg 16860 gaaattcaaa ccgaacccat gaagcccacc tccagcacca ttgaggtgca gacggatcct 16920 tggatgcccg cgcctgctcc tgttaccact actactcgaa gacctagaag aaagtatggt 16980 tcagccaacc tgataatgcc aaactatgct ctgcatccat caatcatacc cactcctggc 17040 taccgcggca ctcgctacta ccgcagtcac agcacccgcc gacgtaaagc acctgccacc 17100 cgccgccgtc gccgccgccg tgccactagc aaacttaccc cctcggctat ggtgcggaga 17160 gtgtaccgtg atgggcgcgc agctcctctg acactgccgc gcgcgcgcta ccatcctagc 17220 attgccattt aacaactctg cctccttgca gatatggccc tcacttgccg ccttcgtatt 17280 cctattgctg gctaccgcgg aagaaagtcg cgccgtagaa gagcagggtt gtctgggagc 17340 gggatgcgtc gccaccggcg gcggcgcgcc atcagcaaac ggttgggggg tggatttctt 17400 cccgctttga ttcccatcat cgccgcggcg atcggcgcga taccaggcat agcttccgtg 17460 gcggtgcagg cctcgcagcg ccactgacat tggaaaaaga tatcttataa ataaaaatag 17520 aatggactct gacgctcctg gtcctgtgat atgtttttgt agacgagatg gaagacatca 17580 atttttcatc cctggctccg cgacacggca cgcggccgta tatgggcacc tggagcgaca 17640 tcggcaacag ccaactgaac gggggagcct tcaattggag cagtctatgg agcgggctta 17700 aaaattttgg gtccactata aagacttatg ggaacaaagc ttggaacagc agcacagggc 17760 atgcgctgag acaaaagctt aaagatcaga atttccaaca gaaggtggtc gatggtatcg 17820 cctctggaat caatggggtg gtagatctgg ccaaccaggc cgtgcagaaa cagattaaca 17880 gtcgcctgga cccggctccc ccagctccta ttcatgagtt aatgcaagtg gaggaagagc 17940 tcccttcatt ggaaaagcgg ggcgataagc gacctcgtcc agatatggag gaaacgctgc 18000 tgaccaaggt ggatgagccg ccctcctatg aagaggctgt aaaactggga atgcccacta 18060 caaagcccat tatgcctctg gccactggag tgatgaagcc atctcagtct aaacctgcag 18120 ttgctgctac attggacttg cccgctcccg tggccacccc caaacctgtc gccgccccga 18180 agcccaccgc cgtgcaaccc gtggccgtgg ccagaccgcg tcccggtggt cggccgaatg 18240 caaactggca gagcactctg aacagcatcg tgggtttggg agtgcacagt gtgaagcgcc 18300 gtcgctgcta ttgattaaat atggagtagc gcttaacttg cttgtctgtg tgtgtatatg 18360 tcgatgccgc ccgccgtgct acagcaaaga gagaaggaga agaggcgccg ctgagttcct 18420 ttcaagatgg ccaccccatc gatgctgccc cagtgggcgt acatgcacat cgccggacag 18480 gacgcttcgg agtacctgag tccgggtctg gtgcagttcg cccgcgccac agatacctac 18540 ttcaatctgg ggaacaagtt taggaaccct accgtggctc ccacccacga tgtgaccacc 18600 gaccgtagcc agcgcctgac gctgcgcttt gtgcccgttg accgggagga caatacctac 18660 tcctacaaag tcagatacac cctggctgtg ggagacaaca gggtgttgga tatggccagc 18720 acctactttg acatcagggg cgtgttggac agaggaccta gcttcaaacc atactctggc 18780 actgcctaca actccctggc tccaaaagga gctccaaact ccagtcagtg gcaacaaaag 18840 gaaaacaatg gtcaaggtga tgcaaagact cacacctatg gtgtagctgc cactggaggt 18900 attgacattg acaaaaatgg tcttcaaatt ggaatcgatg aaactaaaga agataataac 18960 gaaatttatg cagacaaaac attccaacct gaacctcaaa ttggagaaga aaactggcaa 19020 gatagcgaaa actattatgg aggcagggct cttaaaccgg aaaccaagat gaagccttgc 19080 tatggttcct tcgctagacc aactaatgca aagggaggtc aagccaaaat taaaccagct 19140 caagagggtc aacagtctat agattatgac atagacctgg ctttctttga tattccaagc 19200 actggcggag gcaatggcac aaatgtaaat gacaagccag atatggttat gtatactgaa 19260 aatgtaaatc tggaaactcc agacactcat cttgtttaca agccaggaac ttcagatgac 19320 agttccgagg ccaatttaac tcagcaagcc atggctaaca gacccaacta tattgggttt 19380 agagataact ttattggcgt catgtactac aacagcactg gcaacatggg agtgcttgct 19440 ggtcaagcat cccagctaaa tgctgtggtg gacctgcaag acagaaacac cgagctgtct 19500 tatcagctat tacttgactc tctgggcgac agaaccaggt attttagtat gtggaatcag 19560 gcggtggaca gctatgatcc tgatgtgcgc attattgaaa accatggtgt ggaagatgaa 19620 ttgccaaact attgcttccc attggacgga gctggcacta atgctgttta ccaaggagtt 19680 aagacaaaag aggataataa tggcgaatgg gaaacagaca caaatgttgc atcgcagaat 19740 cagatatgca agggcaacat atatgctatg gagatcaacc tgcaagccaa cctgtggaaa 19800 agtttccttt actccaacgt ggctctgtac ctaccagact cctacaagta cactccatcc 19860 aacgtgacac tccctaccaa cactaacacc tatgactaca tgaatggcag ggtggtgtct 19920 ccatccctgg tggatgccta cattaacatt ggcgccaggt ggtctctgga tgccatggac 19980 aatgtcaacc ctttcaacca ccaccgcaat gccggcctgc gctaccggtc catgcttctg 20040 ggcaacggcc gatacgtgcc cttccacatc caagtgcccc agaaattctt cgctatcaag 20100 aacctgctgc ttctcccagg ctcatacacc tacgagtgga acttccgcaa ggatgtcaac 20160 atgatcctgc agagttccct tggcaatgac ctcagaaccg atggggccac catccagtac 20220 accagcatca atctctatgc caccttcttc cccatggctc acaacactgc ctccaccctg 20280 gaagccatgc tgcgcaatga caccaatgac cagtccttca atgactacct ctcagctgcc 20340 aacatgcttt accccatccc tgccaatgcc accaacgtgc ccatctccat cccatctcgt 20400 aactgggctg ccttcagggg ctggtctttc acccgcctca agaccaagga gaccccatct 20460 ctgggatcag ggttcgatcc ctacttcgtc tactcaggct ccattccata cctggatgga 20520 actttctacc ttaaccacac tttcaagaaa gtctccatca tgtttgactc ttctgtcagc 20580 tggccaggca atgacaggct gctgactccc aatgagttcg aaatcaagcg cactgttgat 20640 ggggaagggt acaatgtggc acaatgcaac atgaccaaag actggttcct ggttcagatg 20700 ctctcccact acaacattgg ctaccagggc ttctacatcc cagaaggata caaggaccgc 20760 atgtactcct tcttcagaaa cttccagccc atgagccgcc aggtggtcga tcaggtcaac 20820 tacaaagact acatggcagt cacccttgcc tatcagcaca acaactctgg ctttgtgggc 20880 tacctcgcgc ccaccatgcg acagggccaa ccctaccctg ctaactaccc atacccgctc 20940 attggcaaga ctgcagtcaa cagtgtcacc cagaaaaagt tcctctgcga cagggtcatg 21000 tggcgcatcc ccttctccag caacttcatg tccatggggg cccttaccga cctggggcaa 21060 aacatgcttt atgccaactc cgcccacgcg ctagacatga atttcgaagt agaccccatg 21120 gatgagtcca cccttctcta tgttgtcttc gaagtcttcg acgtggtcag agtgcaccag 21180 ccccaccgcg gcgtcatcga agctgtctac ctgcgcaccc ccttctcagc tggtaacgcc 21240 accacataag cgccttgctt cttgcaagtg gctgcagcag catggcctgt ggatcctcca 21300 ctggatccaa tgagcaagag ctcagggcca tcgccataga cctgggctgt ggaccctatt 21360 tcctgggaac ctttgacaag cggtttccag gcttcatggc tcctgacaag ctcgcctgtg 21420 ccattgtcaa cacggcaggg cgcgagactg gtggtgagca ctggctggct tttggatgga 21480 acccccgctc caatacctgc tatctctttg acccgtttgg gttttcagac gagcgcctca 21540 agcagatcta tcaattcgag tacgaggggc tcctgcgccg cagtgccctg gctactaagg 21600 accgatgcat cactctggaa aagtctaccc agaccgtgca gggtccgcgc tcggctgcct 21660 gcgggctctt ctgctgcatg ttcctccatg cttttgtgca ctggcccgac cgccccatgg 21720 acaacaaccc caccatgaat ttgctgacgg gggtacccaa caacatgctc caatcgcccc 21780 aagtagagcc caccctgcgc cacaaccagg aggcactcta tcgcttcctg aactcccact 21840 catcttactt tcgttctaac cgcgcgcgca ttgagaaggc cactgccttc gatcgaatga 21900 ataataacat gtaaaccaaa ttgtgtgtgg ctcaaataaa cagcacttta ttgtttacat 21960 gcactgaggc tctgggatga tcatttttta aaaatcgaag gggttctggc gggaatcagc 22020 atggccagat ggcagggaca cgttgcggaa ctggaacttg ttctgccact tgaactcggg 22080 aatcaccagc ctgggaactg gaatctctgg aaaggtatct tgccatagct ttctggtcag 22140 ttgcagagcg ccaagcaggt caggagcaga tatcttgaaa tcacagttgg ggccagaatt 22200 ctgggcgcgg gagttgcggt acactgggtt gcagcactgg aacaccataa gggcagggtg 22260 tctcacgctc gccagcacgg tctcgtcact gatgcaagac acatccaggt cttcagcatt 22320 ggccattcca aagggggtca tcttgcaggt ctgtctgccc atcacgggag cgcagccagg 22380 tttgtggttg caatcacaat gaagggggat cagcatcatc ttggcctggt cgggggtaat 22440 ccctgggtaa acagccttca tgaaggcttc atactgcttg aaagcttcct gggctttggt 22500 tccctcggtg tagaacactc cacaagactt gctggaaaac tgattagtag cgcagttggc 22560 atcattcaca cagcagcggg cgtcgttatt agccagctgg accacattcc tgccccagcg 22620 gttctgggtg atcttggctc gatctgggtt ctccttcaac gcgcgctggc cgttctcgct 22680 cgccacatcc atctcaatga catgttcctt ctggatcatg atgttgccat gcaggcatct 22740 aatcttgcct tcataatcag tgcagccatg aggccacagc gcgcacccgg tgcactccca 22800 attgttatgg gggatctggg aatggctatg aaccagccct tgcaggaatc ttcccatcat 22860 cacagccagg gtctttatgc tggtaaaggt cagcgggata ccgcggtgct cctcgttcac 22920 atactgctgg cagatgcgtc tgtagtgctc ggcctgctcg ggcatcagct tgaaagaggt 22980 tttcaactca ttatccagcc tgtatctctc catcatgatg gacattactt ccatgccctt 23040 ctcccaggca gaaacaatag ggagactcag gggattcttg acagtagaga caaccttact 23100 taaggggtca tcactgccaa tcttttcgat gcttctcttg ccatccttct cggtgatgcg 23160 caccggcggg tagctgaatc ccacagccac caactgagcc tcttcccttt cgtcttcgct 23220 gtcttgactg atgtcttgca gaggaacatg tttggttttc ctgggtttct tcttgggcgg 23280 cagctctgga ggactctggc tccgttccgg agaccccatg gatgagcgag agttgtcgct 23340 caccacttgg atctggctgc ctgtagaaga actggacccc acgcggcggt aggtgttcct 23400 cttggtaggc agaggtggag gcgacgggct ccggtccggt ctgggtggcg gatggctggc 23460 ggagcccctt ccgcgttcgg gggtgcgctc cagatggcgg tcgtctgact gacctccgcg 23520 gctggccatt gtgttctcct aggtagagaa acaagacatg gagactcagc catcgctgcc 23580 atcgccatcc accaccacaa gcaccgccga ggaggaggag tgtttaacca ccccaccatg 23640 cagccccgct accaccacca gcacccttga aagcgaggtc gacacggtcg tggaggattt 23700 acaggctatg gaagatattg aggcagctgt cgagcaagac cccggctatg tgacaccggc 23760 ggagcatgat gaggatctag cgcgctttct cgacggtgtg gagaaagcga aacaagatga 23820 ggacgaggaa gaggcagaag cacaaccatc ggtggccgac tacctcaccg gcctagggct 23880 agaagacgtg ctgcttaagc atcttgcaag gcagacagtc atagtcaaag acgccctgct 23940 agagcgctcc gaggtgccac tcagtgtgga agacctcagt cgcgcctatg agctaaacct 24000 cttctcgcct cgcaagcccc ccaagcgtca gcccaacggg acctgtgagc ccaatccgcg 24060 cctcaacttc tatccagcct tcactgtgcc cgaagtacta gctacctacc acatcttttt 24120 caagaaccaa aagatcccca tctcctgccg cgccaatcgc acccgcgcag atgccctact 24180 caacttgggg cccggcgctc gcatacctga tatcgcttcc ttggaagagg ttcctaagat 24240 ctttgagggt ctgggcaatg aggaaactcg ggcagcaaac gctctgcaaa gagaaacaga 24300 tgatggtgaa caccacagcg ctctggtgga gctccagggc gacaacgctc gtcttgcagt 24360 cctcaaacgc agcatcgagg tcacccattt cgcctacccc gcacttaatc tcccacccaa 24420 agtcatgagc tcggtcatgg acacgttgct catgaagcgc gcgagcccca tctccgagga 24480 tcagaacatg caggaccccg atgcctcaga tgaaggcaag cctgtagtca gcgacgagca 24540 actggctcgc tggctaggct ctgactcccc ccagtctttg gaggagcggc gcaagcttat 24600 gatggcagtg gtcctgatca cagcggagct ggagtgtctc cgccgcttct tcactgaccc 24660 agagaccctg cgcaagcttg aggagaacct gcattacaca ttcagtcatg ggttcgtgcg 24720 ccaggcgtgc aagatctcca acgttcaact caccaacctg gtctcctacc tgggcatctt 24780 gcatgaaaac cggctggggc agaacgtgct ccacaccacc ctgaaggggg aggcccgccg 24840 cgactatatc cgcgactgta tctacctcta cctatgctac acctggcaaa gcgggatggg 24900 tgtgtggcaa cagtgcttgg aagagcaaaa tctaaaagag ctggaaaagc tgcttcagaa 24960 atctcttaaa tctctgtgga ccgggttcga tgagcggacc accgcttcgg acatggccga 25020 tattatcttc cccgagcggc tcagacacac tctgcgcgac gggctgcctg actttgccag 25080 ccagagcatg ctacaaaact ttaggtcatt catcttggaa cgctccggga tcctgcccgc 25140 cacttgctgc gcactgccct ccgattttgt gcccatcacc taccgggagt gccccccgcc 25200 gctatggagc cactgctacc tgttccgcct ggccaactac ttggcctacc actctgatgt 25260 gatagaagat gttagtggcg aagggctcct ggagtgccac tgccgctgca acctctgcac 25320 cccccaccgc tccctcgcct gcaatcccca gctgctgagc gaaacccaga tcatcggcac 25380 cttcgagttg caaggtccca gcggcgaagg cgaggggtcc tctccggggc aaagtctgaa 25440 actgactccg gggctatgga cctccgctta ccttcgcaag ttcgccccca aagactacca 25500 cccctatgag atcaggtttt atgaagacca atcacagccc cccaaggccg aactgacggc 25560 ctgcgtcatc acccaggggg caatcttggc ccaattgcaa gccatccaaa aatcccgcca 25620 agaatttttg ctgaaaaagg gacacgggat ctatctagac ccccagaccg gtgaggagct 25680 gaatacacgc ttccctcagg atgccccgag gaggcaagag aatgaaagtt cagatgccgc 25740 ccgaggagga gctggaagac tgggacagtc aggcagagga ggaagactgg gacagccagg 25800 cagaagagga ggacagcctg gaggaggaca gtctggagga aggcgaggag cccaaggaag 25860 aggcagccgc cgccagacca tcgtcctcgg cggtggagac aagcaaggtc ccagacagca 25920 cggctaccac ctccgctcca gctcaagggg ccgctcggcg acccaacagt agatgggacg 25980 agacgggtcg cttccagaac cccaccaccg tcaagaccgg taagcaggag cggcagggat 26040 acaagtcctg gcgggggcat aaaagtgcca tcatcgcttg cttgcaggag tgtgggggca 26100 atatatcctt tgccagacgc tacctgctat tccatcacgg ggtgaatttc ccccgcaaca 26160 tcttgcatta ctaccgtcac ctccacagcc cctactacca gcagcaagag acagcagagg 26220 aaaccagcgg caactccgag agttagaaaa ccagcagcta aaaaatccac agcggcggca 26280 gcaggtgcag gcggactgag gatcaccgcg aacgagccag ctcagaccag ggagttgagg 26340 aatcggatct ttcccaccct ctatgccata ttccaacaaa gtcggggtca ggaacaagaa 26400 ctgaaagtaa aaaacagatc tcttcgctcg ctcacccgca gttgtttgta tcacaagagc 26460 gaagaccaac ttcagcgcac tctcgaggac gccgaggctc tcttcaacaa gtactgcgcg 26520 ctgactctta aagagtagac tgcgcgcgct tggcgagaaa aggcgggaat tacgtcacct 26580 cttggccaca cctgtgcttc attatgagta aagaaattcc cacgccttac atgtggagct 26640 atcagcccca gatgggattg gccgctggcg ccgcccagga ctactccacc cgcatgaatt 26700 ggctcagcgc cggtcccgcg atgatctcac gggttaatgg tgtgagagag caccgaaacc 26760 agatactcct agaacagtcc gccctcaccg ccactccccg caatcacctc aacccccgta 26820 attggcccgc cgccctggtg taccaggaaa ctcctgctcc cactacagta ctacttcctc 26880 gtgacgccca ggccgaagtt cagatgacta actcaggtgt acagctggcg ggtggtgcca 26940 ccctgtgtcg tcaccggcca agaccgggta taaagggcct ggtgatcaga ggccgaggta 27000 ttcagctcaa cgacgagtcg gtgaactctt cgcttggtct gcgaccagac ggcatcttcc 27060 aaatagctgg ttgtgggaga tcttccttca ctcctcgtca ggctgtcctg actttggaga 27120 gttcgtcctc gcagccccgc tcgggcggca tcgggactct ccagttcgtg gaggagttta 27180 ctccctcggt ctacttcaac cccttctccg gttctcctgg gctttacccg gacgagttca 27240 tcccgaacta cgacgccatc agtgaagcgg tcgacggcta cgattaatgt ctaatggtgg 27300 cgcggctgag ctagctcgac tgcgacacct agaccactgc cggcgctttc gctgctttgc 27360 tcgggatctc tgcgagttca tctacttcga gtaccctgac gaacatcctc agggacctgc 27420 ccacggagtt cggattacca ttgaaggggc tatcgattct cacctgcttc ggatcttcac 27480 cgctcggcca gtgctagttg agcgcaacca gggcgacacc accatctccc tctgctgcat 27540 ttgtgacaac cccggattgc atgaaagctt ttgttgtctt ctttgtactg agtataataa 27600 aagctgaaat tagagactac tccggactct cttgtcgtct gaacaacacc aaccagaccc 27660 ttcacttcag cgggaaccag actactcttc actgtaaggc ttataactat aagtatctta 27720 cttggatata caaaggaaca ccgtttgctg tggtaaacag gtgctccaac gacggtgttc 27780 tcctcacctt cctaggcaac ttctccaact ttaccttttc tgttcgcaga aacaagctta 27840 ccctccttca gccctacttt cctgggatct atacctgcct cagtggacct tgcaaccaca 27900 cttttcacct gattgaaaac tctaccctta ccttcccagc gccaatccct actaacagct 27960 cggagtccaa ctcttccatt accgctgata ctaacactcc taaaaccgga ggtgagctcc 28020 gcagccttcc cccggctgca gataaccctt gggtggtagc gggatttgta gcgctaggaa 28080 tagttgcggg tgggctcgcg ttcgtcctct gctacctata ccttacctgc tgctcatatt 28140 tagtagtact gtgctgttgg tttagaaaat gggggcgcta ctaatcacac ttgctttact 28200 ttcgcttttg ggtctgagct cggctaatag cgagaaacca agctgtctag aaacaaactc 28260 tccagactgt gtggttcctc atgggctctc agacccagct gatgatccat gcttaacttt 28320 tgacccagaa aaaaactgct cggtgactat gcagccctat gcttacatgt gcacatctgt 28380 tataaagtgc ggatggggct gtaaaccgat tgaaattacc cacaaaggca aaacctggaa 28440 taatagtttg tttaacacat ggcagcctgg agacgagcag tggtatactg tcactgtccg 28500 tggtcctgac ggctcagtta aagctcatta caatctaacg ttcatgtttg cagaattgtg 28560 tgatctggct atgatcatgc aaagacagta caggctgtgg ccccccacta aggaaaacat 28620 tgttgagttc tccattgctt actgcatagg cacctgcttg gtcactgcta tcatgtgcgt 28680 ctgcattcac ctgcttgtaa tcattagacc caggaataac aatgaaaagg aaaaaatgcc 28740 ataatctttc taactttgtt ttcagccatg attactttta caactattct tgctatcatt 28800 agcattgtga ctgctcaaag gcacccacac acaacactaa acatttacac tggtcaaaac 28860 tatacattgt ggctaacaag caactactca aatgttatgt ggatatatca tacaaaatca 28920 tggaccaaca gccaaatgct atgtcgcggt accacaactt catacccaga gcttaagcac 28980 acttgtactg acacaaactt aacacttatt aatgtaaatg caaccttcaa tgccgactat 29040 tatgcatata gcggcaccca ttctttccaa tttacaattc acgttcacaa cttaaccaca 29100 ccaaaaccac caacaacact tacaacaact acaaccagca caaccacagc cagaactaaa 29160 cctacaacag gcaagcagtc cactaaaaca attcatacac ttggtttctt ggaaacattg 29220 ttttttgcta gaagcagttc taacaactct ggaaatgcaa cactagctga tgaagctcaa 29280 attcctggtt ctatgattgg aattgttatg gcagtagtga tagtaatggt gattattatc 29340 attagcatga tcacctatgc ttgctgctat aggaaattcc atcctacaaa agttgatcca 29400 ttattaaatt ttgattttta atcctacaga acaatgaaaa agctgcttat aattttgctt 29460 ttaaaattct catttggagg aaagttatac acgcgcataa caacaactga aggacaaaac 29520 ttaacattgc taggtgtaaa tgctattaat aataatgttt acaaaactgt caacataaca 29580 tgggaaactg aaattaatgg aactaggcaa aatgtgtgca aactaaacat aacaaaatat 29640 acatgtaagg cttatgattt aataatagtt aatgttacca cacttgattc aaaattatac 29700 gtagggcaaa gctacactgt ttttgcaaaa ggattaacta aaggctatac tatacataac 29760 cttgcagcac accaagtgac agttattaaa gcaacaacac cccgcaccac aactgtcaca 29820 acacacaaaa gcaccgcttt gttacaatca atacacagta cgcctactat acctcaacca 29880 attgctgaag cagtgcactt taataatcat gaaaccaata tgcagactac tgaaaaaatc 29940 actagcaaaa aacataccac tattgctgtg cttatgccaa cacctgccaa aatctttttg 30000 atgccaatat cagttcaaat cgcaataatt gctgtaatag gcattgtgat tatttcttgt 30060 ctgctttatt atgttttctg ctgtcataaa cactatactg aaagaaaacc catttataga 30120 ccaatgatag gggatccaga acctcttaag gtggagggtg gtctaagaaa tcttctcttc 30180 tcttttacag tatggtgaac tgcaatcatg atccctaggc aatttttctt caccatacta 30240 atctgtgctt tgaatgtgtg tgcaactctc gctgtagtag ccaacacaaa cccagagtgt 30300 ctaggcccct tcgctaccta cctgctattt gtctttatta cctgcacctg catctgtagc 30360 atagtctgcc tgattattaa cttctttcaa ttcatagatt gggtatttgt gcgaatttgc 30420 taccttcgcc atcacccaga ataccgcaac aaagatgtcg ctgaactgct caggctgctc 30480 taaaatctgg caggctatga tactgctttt gcttcagctt ctgcttatac ttaccgcagc 30540 ttcagcagca acccctaccc ccaaacccga tgtaagcaag tgtaaattcc accagccatg 30600 gactttcctc caatgctacc atctcaaatc tgagttgccc tcttattaca ttgcgatcat 30660 tggaatcctc accatgctat catgcacatt cttctcattc atgatttacc ccacatttga 30720 ttttgggtgg aatgcaccag attcacttag gctcccacaa tacccagacc cagagccaga 30780 acacattgca ctacagaaca tgcccatacc catattagag tatgaagctg agccacaaca 30840 ccccatgctc cctgctatta gctacttcaa cctaactggt ggagatgact gaccccatgg 30900 aaaactcctc tgccaacgac ctggacatgg acggccgttc atctgagcag cgactggtcc 30960 agatgcgcat tcgccagaag caggaacgcg ccgccagaga gctcaaggat gccattgaaa 31020 ttcacctgtg caagaagggc atcttttgct tggttaagca agcaaagatt tcttatgaaa 31080 tcactgacaa cgaccaccgc ctgtattatg agctcggtcc acagcggcag aaattcacct 31140 gcatggttgg agtcaacccc atagtcatca ctcagcaggc tgcagaaatt aaagggtgca 31200 tccactgttc ctgtgattcc caagaatgcg tccacaccat agtcaagacc ctctgcggcc 31260 ttcgagatct tcttccaatg aactaacccc ttcccccaac ccaataaaac attggtttta 31320 atcataataa aaaatcactt actttaaatc tgaaacagtg tctccgtcca agttttcttg 31380 tagcaccact tcactcccct cttcccagct ctggtactgc aagccccggt gggctgcaaa 31440 ctttctccac accttaaaag ggatgtcaaa ttcctcttgt ccaacaatct tcattgtctc 31500 ttcctagatg tccacaaagc gcgcgcgggt ggaagatgac tttgaccctg tctacccata 31560 cgatgctgag ctggcaccgt ctgtaccctt catcgcccct cccttcgttt cgtcagacgg 31620 atttcaagaa aaacccctgg gagttctgtc cctaagacta gccaacccag tcactactaa 31680 aaatggggaa ctcacactta aactgggaga tggggtgggc atagactcag atggaaacct 31740 cacagcacag acagttacta aagcaacatc cccccttact gtttccaata acgcaattgc 31800 acttaacatg gacaaacctt tttacagtag caatggaaaa ctatccttac aagttacatc 31860 accattaaag atagtcgact ctttaaatac attggctatt ggctatgggc aaggcttagg 31920 actaaacaat agtgctcttg ctgtgcaatt agcatctccc cttacatttg acagcaacag 31980 caaaattaaa ataaatttgg gaagcgggcc attaaaaatt aatgcgaata aactgtcaat 32040 taactgccta aggggtgtat atgtaacaac tgacggaact tcaattgaaa caaatataag 32100 ctgggcaaaa ggaatgaggt ttgaaggtaa tgccatggct gtaaacgttg acagcaccaa 32160 aggtctacaa tttggcacta ccagcacaga atcaggagtc actaacgctt tccctatcca 32220 gttaaagatt ggatctggtc ttagttttga cagcacagga gcacttgtag cttgggataa 32280 ggataatgac aagcttacac tgtggacaac cgctgaccca tcacctaatt gtaccatata 32340 tacagacaag gatgctaaac ttacactttg tcttacaaaa tgtggcagtc aaatactagg 32400 cagtgtttca gtactggctg ttaaagctgg aaccctacag ccaatcagtg aaaaaatagg 32460 tactgctttg gtttcactaa aatttaataa caacggtgta ttgttaagca actccacatt 32520 aagtaatgaa tactggaact acaggaaggg agatgtcaca ccagccgaag cctatactaa 32580 tgctgtgggt tttatgccaa acatcaaggc atatcctaaa aacacaaact ctgcctcaaa 32640 aagccacatt gtaggacaag tgtaccttaa tggagatgaa actaaaccaa tgcatttaat 32700 cattacattt aatgaaacca gtgatgaaac atgcacatat tccataacgt tccaatggaa 32760 atggaacatt ggaacataca ccagcgacac ccttgcaaca agctccttta ccttttctta 32820 cattgcccaa gaataaaaac tgcagacaac aataaagttt aaatgtttta tttaaacagt 32880 tttacacgat tcgagtagtt attttgcctc ccccctccca tttaacagag taaactagtt 32940 tttgcccttg aacagcttta agcaatttta tcccactaga gatagataga tttttagttt 33000 ctacattcca cacagtttca aaacgagcaa atctaggatc agtaagggaa agaaattcat 33060 ctggacaatc ttttagggca gtggcttcac gatcttgctg ctggaaatgt tcatcagtag 33120 tagttgtagg aatcagagtc atctggaaga agaacggtgg gaatcataat ccgaaaatgg 33180 aattgggcga tggtgtctca ttaaaccctg gagcagtctc tgccgatgtc tctcagtgcg 33240 gctgctgctg atgggatctg gatccatagt ctctcgaagc ataatgccaa tagcctttag 33300 catcagcctt cttgtgcgcc ttgcacagca acgcatcctg atctcactca gatcccaaca 33360 gtaagtacag cataacacca caatgttatt tagcaaacca taattaaatg cactccatcc 33420 aaaactcatg gcagggataa cagagccggc atggccatca taccagattc ttacataaat 33480 cagatggcgc cccctaaaga aaacactgcc catgtacata atctctttag gcatgtgaag 33540 gtttacaacc cttcggtacc atggacagcg ctggttaatc atgcatcctt ctataatcct 33600 tctaaaccag atagccaaca cagcccctcc tgccatgcac tgaagggatc cctgattaga 33660 acagtgacaa tgaataaccc acctttccct cccatgaatt acttgggagt aaaaaatatc 33720 tattgtagca cagcacaagc agacattcat gcattttctc attactctaa actcctcagg 33780 tgttaacacc atatcccagg gtacaggaag ctcttgaatg acagtgaaca tagcagaaca 33840 aggtaaacct ctaacataac ttacactatg catagtcaag gtgttacaat ccggaaggta 33900 tggatgttcc tcagtcatag tagctctggt ctcagtctcc tcacatcgtg gtaactggac 33960 cctgtatggg tgacggcgag cggacgacgt cgatcgtccc cgcgacttcg ttgtagtgga 34020 gcgccttccg gacattctcg tactttgagt ggcaaaacct tgctctcgaa cagcacacgt 34080 ctcgtcgcct cctgtccctt ctcttcgcct tttcagtgtg atagttgtaa tacagccatt 34140 cacgaagctc agtcagaaga tcttcagcgt ctgttgtcaa aaacaatcca tccaatctga 34200 ttgctttcaa aacatcacaa acagtcgaat aagccaaacc catccaggca atgcaattat 34260 tttggttatc cacaatggga gggggcggaa gacatggaag aggcatattt aaatttttaa 34320 tccaatcgat cacgcagcac ttcaaaatga agatcgcgaa ggtgacacct ttcaccccca 34380 ctgtgttgat gaaaaataac agccaagtca aaattgatgc ggttttcaag gtgctcgact 34440 gtagcatcaa gcagagcttc cacacgcacg tccacaaata acagaatagc aaaagcggga 34500 ggaggaagta aatcctcaat catcatagta cagtccatca ccatccctaa ataattttca 34560 tccttccagc cttggactat atttttaaac tgctcttgta aatccaaacc acacatgtgg 34620 aaaagttccc aaagagctcc ctcaactacc attcttaagc acaccttcat agtgacaaaa 34680 tatcttgttc ctctgtcacc tgcagcaaat tacaaagtcc aatattagga tctatgccca 34740 gagatctaag ctcatccctc aattccaact gtaaaaaggc ttccagatct gccctaactt 34800 gttcagccag tgggctccct ggaataagcg tgggagaagc caaactgcaa aacagacgca 34860 tgccgccata attaccacca gaaaacacta cgttacagta tgcatgctga ttcattccag 34920 taatttcatc cagtgtattg gatacaaaaa aaggcaagca ctctctcact aattgtatta 34980 tggagacatt atcacacagg taacaattta aaggttgtgg aacaataatg cagtaagtaa 35040 ccacggtgcg ctccaacatg gttagtaatt tttagttctg aaaaacaaaa catacaaaaa 35100 attatatcat actcatttgg cgaactggtg gaaaaatgac cctatctagc acaaggcaag 35160 ccactggatc accaatgcgc ccctcataaa acctgtcatc atgattaaaa agcaacaccg 35220 aaagctcttc cctatgtcct gcatgaatga ttctagctga ggaatataag ccagcgcaat 35280 tagtatctgt taaagaaaaa aaacggccaa catagcctct aggaattagc acacttaatc 35340 ttaaagacat tactgccatc ccccttggat ttaaggtaaa atttacagga gcatagaaaa 35400 tatactgatt tccctcctgc acaggcagca tagcaccagg tccctctaaa aacacacaca 35460 aagcttctgc agccatagct taccgcgcaa accaggcaca gcagtgagct aaaaggacaa 35520 agctctaact cactagccaa cctggcgcac aatatatagt tagtccttac actgacgtaa 35580 ccgaccaaag tctaaaaacc ccgccaaaaa tacacacacg cccaaaaaac gccccgtgag 35640 tcaaaaaaca gtttcacttc ctcgttacac ccaaaacgtc gtcacttccg gattcccacg 35700 gttcgtcact tccggagctc cttgcaaccc cgcccaaaac gtcatcgtcc gcgtcacgcc 35760 gccccgcccc gcgaccgttg accccgggcc aatcaccgca catcccgcaa aattcaaact 35820 cgtctaattt gcatattggc acactgccca tataaggtat attattgatg atg 35873 <210> 6 <211> 1164 <212> DNA <213> Artificial Sequence <220> <223> HAdV-5 E4 orf 6/7 <400> 6 tcacagaacc ctagtattca acctgccacc tccctcccaa cacacagagt acacagtcct 60 ttctccccgg ctggccttaa aaagcatcat atcatgggta acagacatat tcttaggtgt 120 tatattccac acggtttcct gtcgagccaa acgctcatca gtgatattaa taaactcccc 180 gggcagctca cttaagttca tgtcgctgtc cagctgctga gccacaggct gctgtccaac 240 ttgcggttgc ttaacgggcg gcgaaggaga agtccacgcc tacatggggg tagagtcata 300 atcgtgcatc aggatagggc ggtggtgctg cagcagcgcg cgaataaact gctgccgccg 360 ccgctccgtc ctgcaggaat acaacatggc agtggtctcc tcagcgatga ttcgcaccgc 420 ccgcagcata aggcgccttg tcctccgggc acagcagcgc accctgatct cacttaaatc 480 agcacagtaa ctgcagcaca gcaccacaat attgttcaaa atcccacagt gcaaggcgct 540 gtatccaaag ctcatggcgg ggaccacaga acccacgtgg ccatcatacc acaagcgcag 600 gtagattaag tggcgacccc tcataaacac gctggacata aacattacct cttttggcat 660 gttgtaattc accacctccc ggtaccatat aaacctctga ttaaacatgg cgccatccac 720 caccatccta aaccagctgg ccaaaacctg cccgccggct atacactgca gggaaccggg 780 actggaacaa tgacagtgga gagcccagga ctcgtaacca tggatcatca tgctcgtcat 840 gatatcaatg ttggcacaac acaggcacac gtgcatacac ttcctcagga ttacaagctc 900 ctcccgcgtt agaaccatat cccagggaac aacccattcc tgaatcagcg taaatcccac 960 actgcaggga agacctcgca cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg 1020 cagcagcgga tgatcctcca gtatggtagc gcgggtttct gtctcaaaag gaggtagacg 1080 atccctactg tacggagtgc gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc 1140 aaatggaacg ccggacgtag tcat 1164 <210> 7 <211> 99 <212> DNA <213> Artificial Sequence <220> <223> HAdV-5 protein IX promoter <400> 7 ggtactgaaa tgtgtgggcg tggcttaagg gtgggaaaga atatataagg tgggggtctt 60 atgtagtttt gtatctgttt tgcagcagcc gccgccgcc 99 <210> 8 <211> 33040 <212> DNA <213> Artificial Sequence <220> <223> pBLY6.dE1.dE3 <400> 8 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg atggcctgtg tttgagcata 480 atgtactgac caggtgtaac gttcatctgg gtggtcgtag aggaatgttc atgccatacc 540 aatgcaattt taatcatgtg aggatcttga tggagccgca agcgttttcc agagtcagct 600 tgactggaat ctttgacatg tgtgtggaag catggaagat cttaagatat gatgatacca 660 aatccagatg ccgcgcatgc gagtgcgggg gcaggcatgc caggttccaa cctgtatgtg 720 tggaggtgac cgaggagctg agaccagatc atttggtgct gacctgcact ggtgcggagt 780 tcggttccag tggtgaagaa actgattaaa gtgagtagtg ggatgttata aaagtgacca 840 taaggtgatg tgagatggac aaatttggta atttttatgt atttttgtct tgcagccatg 900 agtgggagcg cttcctttga agggggcgtc tttagccctt atctgacggg gcgtctgcct 960 cattgggctg gagtgcgtca gaatgtgatg gggtctacag tggatggaag acctgttcag 1020 cctgctaatt cttctactct gacttatgct actatgactt cctcgccttt ggatgcagct 1080 gcagctgctg ccgcttctgc tgccgccaac actgttcggg ggatggcctt ggagatgggg 1140 tattatggaa ctgtagtggc caacaccact accccaaata accccacagc cttgaatgag 1200 gacaagctgc tagttctcat gtcccagctg gagtctttga cccaacgcct gggcgatcta 1260 gctcagcagg tgtcccagct gaaggagcag actcaagctg caattaccac tgcgagggga 1320 aattaaaaaa attcaaagaa tcaataaata aaccgagact ttgttgattt taaagtgtgt 1380 cattctttat ttaatttttc gcgcgcgata tgccctggac caccggtctc tatcattgag 1440 gacacggtgg atcttttcta gaacccgata gaggtgggat tggatgttga ggtacatggg 1500 cataagacca tctttggggt gtagatagct ccactgcaga gcctcatgct ccggggtggt 1560 gttgtatata acccagtcat agcatgggcg ttgggcatga tgttgcacaa tatctttaag 1620 gaggagacta atggccactg ggagaccctt ggtgtaagtg tttacaaatc tattaagctg 1680 ggacgggtgc atccgaggtg agataatgtg cattttggat tggattttta gattggcaat 1740 gtttccccct agatctctcc tgggattcat gttatgcaag accactagaa cagtgtatcc 1800 ggtgcactta gggaatttgt catgaagttt ggaggggaaa gcatgaaaaa atttagacac 1860 acccttgtgt cctcccaagt tctccatgca ctcatccata ataatggcaa tgggcccatg 1920 ggcggcggca cgggcgaaca cgttcctggg atctgacaca tcatagttgt ggtcttgggt 1980 caggtcatca taagccattt taataaactt ggggcggagg gtgccagatt gggggatgaa 2040 tgttccctcg ggccccggaa catagtttcc ttcacatatt tgcatttccc aggcttttag 2100 ttcagagggg gggatcatgt ccacctgtgg agcgatgaag aagacggtct cgggggcggg 2160 ggtgattaag tgggaggaca gcaagttcct aagcagctgt gacttgccac acccagtggg 2220 accgtagatg acccctataa caggttgcag atggtagttt agggaaagac agctgccgtc 2280 ctctcgcagg aggggggcga cctcgttcat catttccctc acatgcatgt tttcccgcac 2340 aagttccgat aggaggcgct ctccacccag ggaaaggagt tcttgaagag atgagaaatt 2400 tttcaagggt tttaagccat cagccatggg cattttggag agggtttgtt gcaagagttc 2460 aaggcggtcc cagagttcgg tgatgtgttc tatggcatct cgatccagca tacttcctcg 2520 tttctggggt tgggacggct gcgggagtat ggaaccaggc gatgggcgtc cagcgctgcc 2580 agtgtccggt ccttccacgg tcgcagcgtc cgagtcaggg tcgtttccgt cacggtgaag 2640 gggtgcgcgc ctggctgggc gcttgcgagg gtgcgcttca ggctcatcct gctcgtggag 2700 aaccgctgcc gttctgcgcc ctgtgcatcg gccaggtagc aattaaccat gagttcgtag 2760 ttgagcgcct ctgccgcgtg gcctttggcg cgcagcttac ctttggaagt cttctgacag 2820 gtgggacagt agagacactt gagagcatag agttttgggg ctagaaagac cgattctggg 2880 gagtatgcat cggccccaca ggaggcgcag acggtttcgc attccaccag ccatgtaaga 2940 tcgggctcgt tggggtcaaa aacaagtttt ccgccatgtt ttttgatgcg tttcttacct 3000 ttgctttcca tgagttcgtg cccccgttgg gtgacaaaga ggctgtccgt gtccccgtag 3060 actgacttta tgggcctgtc ctcgagcggc gtgccgcggt cctcttcgta gaggaactcg 3120 gaccactctg agacgaaagc acgtgtccag gccagcacaa aggaggctat atgggagggg 3180 tagcgatcgt tgtcaaccaa ggggtctact ttttccaagg tgtgtaaaca catgtcccct 3240 tcttccacat ccaggaaggt gattggcttg taagtgtatg ccacgtgacc tggggtccca 3300 gacggggggg tataaaaggg ggcgggtctc tgctcgtcct cactgtcttc cggatcgctg 3360 tccaggagcg ccagctgttg aggtaggtat tccctctcga aggcgggcat aacctccgca 3420 ctcaggttgt cagtttctag gaacgaggag gatttgatat tgacagtgcc tgccgagatg 3480 cctttcatga gactgtcgtc catttggtca gaaaagacaa tctttttgtt atcaagtttg 3540 gtggcgaagg atccatacag ggcattggaa agcagtttgg caatggagcg catggtttgg 3600 tttttttctt tgtctgcgcg ctctttggcg gctatgttga gttggacata ttcgcgggcc 3660 agacatttcc attgtggaaa tatggtagtt aattcatctg ggacgattct gactttccag 3720 cctctgttat gcagggtaat cagatccaca ctggttgcca cttctcctct aagtggttca 3780 ttagtccagc atagtcgccc cccttttcga gaacagaaag ggggtagggg atctagcatg 3840 agttcgtctg gggggtctgc atctatggtg aaaatcccag gaaggagatc ttcgtcaaaa 3900 tagctgatgg tggcggggtc atccagagac atttgccatt ctcgagcagc cagagcgcgc 3960 tcgtaggggt taaggggagt cccccatggc atgggatggg tgagtgcaga agcatacatg 4020 ccacagatgt catagacata gagcggctct tccagaatcc ctatgtaagt gggataacat 4080 cgcccccctc tgatgctggc tcgcacataa tcatagagtt catgtgaggg cgctagaaga 4140 cccgagccca ggttggtgcg gttgggtttt tctgctctgt agaggatctg gcgaaagatg 4200 gcatgggagt ttgatgagat ggtgggtctt tggaagatgt tgaaatgggc atgaggcagt 4260 cccacagagt cccttatgaa gtgagcatag gagtcttgca gtttggccac cagctcggcg 4320 gtgaccagca catccaaagc acagtagtcg agggtctctt tgatgatgtc atagttaggt 4380 tcccctttct tttcccacag ctcgcggttg agaaggtatt cttcgcgatc cttccagtac 4440 tcttcgaggg ggaacccgtc cttgtctgaa cggtaagaac ccagcatgta aaattgattg 4500 acagctttgt aggcacaaca ccccttctcc acggggagtg agtatgcttg cgcggctttg 4560 cgcagagagg tgtgagtaag ggcgaaagtg tccctgacca tgactttgag gaactgatgc 4620 ttaaagtcta tgtcatcgca ggccccctgc tcccacagtt ggaagtccac tcgctttttg 4680 taggcgggat tgggcaaagc gaaagtaaca tcgttgaata ggatctttcc agccctgggc 4740 atgaagttgc gagtaatgcg aaaaggctga ggcacttctg ccctgttgtt gataacttgg 4800 gcagccaaga cgatctcgtc aaagccgttg atgttgtgac ccacaatgta aagttctacg 4860 aagcgtgggc gtcccttgat gtggggcagt tttttaagct cttcgtaggt caagtcgtca 4920 gggtcagcga ttccatattg ctccaaagcc cagtcaggca ggtgaggatt agcatgaagg 4980 aaagaggtcc aaagatccac ggccagagct gtttgtaagc ggtctctgta ctgacggaaa 5040 tgtcggccta ccgccatttt ttcaggagta acacagtaaa aggtgcgcgg gtccttttcc 5100 cagcgatccc attgaagttg caaggctagg tcgtgggcga ggttgacgag ctgttcgtcc 5160 cccgaaagtt tcatgaccag catgaaaggg acaagctgct tgccaaagga ccccatccag 5220 gtgtaggttt ccacatcgta ggtgaggaag agcctttctg tgcgaggatg agaaccgatc 5280 gggaagaact ggatttcctg ccaccagttg gaggaatggc tgttgatatg atggaagtag 5340 aactccctac ggcgcgccga gcattcgtgc ttgtgcttgt acagacggcc acagtactcg 5400 cagcgctgca cgggatgcac ctgatgaatg agctgtacct ggcttccttt gacaagaaat 5460 ttcagtggga agttgaggcg tggcgtctgc atctcgtgtt gtattacgtc ctggctattg 5520 gtctggccat cttctgtctc gatggtggtc atgctgacga gcccgcgcgg gaggcaggtc 5580 cagacctccg cgcggacggg tctgagagcg aggacgagag cgcgcaggcc ggaactgtcc 5640 agggtcctga gacgctgcgg agtcaggtca gtagggagag tacataggtt tacttgcata 5700 agtttttcca gggcatgtgg gaggtcaaga tgatatttga tttctactgg cgagttggtg 5760 gagacatcga tggcttgcag ggtcccgtgc ccctggggtg ctaccaccgt cccttttttt 5820 ttcttgatcg ggggcggtgt tgcttcttgc atggtaaggt cgtcttctag aagcggcggc 5880 gaggtcgcgc gccgggtggc agtggcggtt ctggacctgg aggtagaggc ggtagaggta 5940 cgtcggcgcc gcgcgcgggt aggttctggt actgcgccct gagaagactt gcgtgagcga 6000 cgacgcggcg gttgacgtcc tggatctgac gcctctgggt gaatgctacc ggacccgtga 6060 gcttgaacct gaaagagagt tcaacagaat caatttcggt atcgttgacg gctgcctgcc 6120 gcaggatttc ttgtacgtcg cccgagttgt cttggtaggc gatctcggcc atgaactgct 6180 cgatctcttc ttcttggaga tctccgcggc ccgctcgttc tacggtggca gcaaggtcgt 6240 tggagatgcg ccccatgagc tgtgagaatg cattcatgcc cgcctcgttc cagacgcgac 6300 tgtagaccac ggctccctcg ggatctctgg cgcgcatgac cacttgggcg aggtttagtt 6360 ccacgtgtct ggtgaagacc gcatagttgc agagacgctg gaagaggtag ttgagcgtgg 6420 tggcgatgtg ctcggtgaca aagaaataca tgatccagcg acgaagcggc atctcgctga 6480 tatcgcccag ggcttccaac cgttccatgg cttcgtaaaa gtccacggcg aagttgaaaa 6540 actgggagtt gcgagcggac acggtcaact cctcctccag aagacggatg agctcggcga 6600 tggtggcgcg cacttcgcgc tcaaaggctc ccgggatctc ttcctcctct tcttcttcca 6660 actcttcctc cactaacatc tcttctactt cctcctcagg cggcgggggt ggaggagggg 6720 gcgcgcggcg acgccggcga cgcacgggca gacgatcgat gaagcgttcg atcacttctc 6780 cgcggcggcg acgcatggtc tcggtgacgg cgcgcccgtc ctccctgggt cgcagagtga 6840 agacgccgcc gcgcagctcc ctgaaatggt gactgggagg gtccccgttt ggtagggaca 6900 gggcactgat gatgcatctt attaattgcc ctgtagggac tccgcgcaag gacctgagcg 6960 tctcgagatc cacgggatct gaaaatctct gaacgaaggc ttcgagccag tcgcagtcgc 7020 aaggtaggct gagcactgtt tcttcggggc gggctgctga gctagagggt tgtacgatgc 7080 tgctggtgat gaagttaaaa taggcagttc tgagacggcg gatggtggcg aggagcacca 7140 ggtctttggg tccggcttgc tggatgcgca ggcggtcggc cattccccat gcattatctt 7200 ggcacctggc cagatcttta tagtagtctt gcatgagtcg ctccacgggc acttcttctt 7260 cgcccgctct gccgtgcatg cgtgtgagtc cgtaccctct ctgtggttgg acgagcgcca 7320 ggtcggcaac gaccctttcg gctagaatgg cttgctgcac ctgggtgagg gtgttctgga 7380 aatcatcaaa gtccacaaag cggtggtagg cccccgtgtt gatggtgtag gagcagttgg 7440 acatgaccga ccagttgact gtctggtgtc ctggtcgtac gagttccgtg tacctgagcc 7500 gcgagtatgc gcgggagtcg aagatgtaat cgttgcaggt tcgcaccagg tactggtagc 7560 cgatgaggaa gtgaggcggc ggctggcggt agagaggcca tcgttcggtg gcgggcgcgc 7620 cgggcgctag gtcttctagc atgagacggt ggtatccgta gacgtacctg gacatccagg 7680 taataccggc ggcggtggtg gaggcgcgcg gaaactctcg cacgcggttc cagatgttgc 7740 gcagcggcat gaagtagttc atggtgggca cggtctggcc cgtgaggcgc gcgcagtcat 7800 tgatgctcta gatacgggca aaaacgaaag cgttgagcgg ttcccttccg tggcctggag 7860 gaacgcgaac gggttaggtc gcagcgtacc ctggttcgag actaaagaaa gcgagcaact 7920 cgaaccggca gagtcgcggc taacgggtat tggcaatccc gtctcgaccc aagccagcaa 7980 atccaggata cggatggggg ccccttttgt ttttcagggc atgagtcacc ggttaaggtt 8040 tacaacggct gtttcatgcc tttagaagtg gctcgcgccc gtagtctgga gaatcaatcg 8100 ccagggttgc gttgcggcgt gccccggttc gagcctgcag cttgagtcgg ccggtgaccg 8160 cggcaaacga gggcgtggcg gccccgtcgt ttctaagacc ttgctagccg acctctccag 8220 tttacgggaa cgagccccct tttatttttt ttgtttttgc cagatgcatc ccgtactgcg 8280 gcagatgcgc ccacagcccc cacagcagca gcagcaggct ggcctacctt ctctacctca 8340 gccgctacct gcaactaccg cggtggccgc tgtaagcggg gccggacagc aggcggctcc 8400 tcaatatgaa ttggacttgg aagagggcga gggattggca agattggggg cgccctcgcc 8460 cgagcgccac ccgcgggtgc agatgaaaaa ggacgttcgc gaatcttacg tgcccaagca 8520 gaatctgttc agagacagaa gcggcgagga gcccgaggag atgcgcgcgt cccgttttaa 8580 cgcgggtcgc gagctgcgac aaggactgga tcgaaaacgg gtgttgaggg atgatgattt 8640 tgaggtggat gaaatgacag ggatcagccc cgctcgcgct cacgtggctg cagctaatct 8700 ggtgacagct tatgagcaga ccgtgaagga ggaaagcaac ttccagaaat cattcaataa 8760 ccacgtgcgc accctgatcg cacgcgagga ggtgaccctg ggcctgatgc acctgtggga 8820 tctgctggaa gccatagtgc agaaccccac tagcaaaccc ctgactgctc aactgtttct 8880 ggtggtgcag cacagcaggg ataatgaggc attcagagag gcgctgctga atatcactga 8940 acctgagggg agatggctgc tggatctggt gaatatcctg cagagcattg tagtgcagga 9000 acgcagcttg cctttgtccg agaaggtggc ggcgatcaat tactctgtgc tgagtctggg 9060 caaatactat gccaggaaga tctacaaaac cccttacgtg cccatagaca aggaagtgaa 9120 aatagatggg ttttacatgc gcatgaccct gaaagtgcta accctgagcg atgacttggg 9180 agtgtaccgc aacgacagga tgcaccgcgc ggtgagcgcc agcaggaggc gcgagctgag 9240 cgacaaagaa ttaatgcaca gcttgcaacg agccctgacg ggagccggga cggaggggga 9300 gaactacttt gacatgggtg cagacttgca ttggctgcct agtcgcaggg cattggaagc 9360 ggcaggcgat gggccctatg tagaggaagt agtagacgag gacgatgagg agggcgagta 9420 cctggaagac tgatggcgcg acccgtattt ttgctagatg gaacaggcgc cggaccctgc 9480 gatgcgggcg gcgctgcaga gccagccgtc cggcattaat tcctcggacg attggaccca 9540 ggccatgcaa cgcatcatgg cgctgacgac ccgcaacccc gaagccttta gacagcagcc 9600 tcaggccaac cgcctttcgg ccatcctgga ggccgtggtg ccctctcgct ccaaccccac 9660 ccacgagaag gttctggcca tcgtgaatgc cctggtggag aacaaggcca tccgctccga 9720 tgaagccggg ctggtataca acgccttgct cgagcgcgtg gctcgctaca acagcagcaa 9780 tgtccagact aacctggaca ggatggtgac cgacgtgcgc gaggccgtgt cccagcgcga 9840 acggttccat cgcgagtcta acctgggttc catggtagcg ctgaacgctt tcctcagttc 9900 ccagcctgcc aatgtgcccc ggggacagga agactatacc aactttatta gcgccctgag 9960 actcatggta gccgaggttc ctcagagcga ggtgtaccag tccggtccag actacttttt 10020 ccagacaagc aggaacggta tgcagacagt gaacttaagc caggctttca agaacctgca 10080 agggctgtgg ggagtccaag ctccagtggg cgacagggcg accgtgtcga gcctgttgac 10140 tccaaattcc cgtttgctgc tgctgctggt gtcccccttc actgacagcg gcagcataaa 10200 cagaaactcc tacttgggct acctgataaa cttgtatcgc gaagctatag gtcaggccca 10260 cgtggacgaa cagacctatc aggagatcac taatgtgagt cgcgctctgg gccaggacga 10320 ccctggaaac ctggaagcta ctctaaactt tctgctgacc aaccgctcgc aaaaaatccc 10380 tcctcagtat acattaactg cggaggagga acggatcttg agatacgtgc agcagagcgt 10440 gggtctgttc ctgatgcaag agggtgcgac ccctagcgcc gcgcttgata tgacagcgcg 10500 caacatggag cccagcatgt atgccagcaa cagaccattc attaataaat tgatggatta 10560 cttccatcgc gcggccgcta tgaactctga ttacttcacc aatgctattc tgaaccccca 10620 ttggctgcct ccgcctggtt tttatactgg cgagtatgac atgcctgacc ccaacgatgg 10680 gttcttgtgg gacgatgtgg acagcgtggc gttctcgcct accgctcctc gtactttttg 10740 gaagaaggaa ggtagtgaca gaagaccctc ctccgtgctg tcaggacgtg agggtgctgc 10800 cgcggcggtc cccgatgctg caagcccctt tcccagtctg ccattttcac taaacagcgt 10860 gcgcagtagc gagctgggga gaataacccg ccctcgcttg ctgggcgagg acgagtattt 10920 gaatgactcc ctactgagac ccgagcggga aaagaacttc cctaataatg ggattgaaag 10980 cctggtggat aagatgagca gatggaagac ctatgcccag gagcacagag atgagcctag 11040 aatcttgggt cctacagtag gcacccgcag acgccagcgc catgatagac agcggggtct 11100 ggtgtgggac gatgaggatt ctgcagatga cagcagcgtg ttggacttgg gcgggagggg 11160 aggtgtgggc aacccgttcg cacacttgcg tccccgtatt ggacgcatga tgtaaaagtg 11220 aaaataaaaa aggaactcac caaggccatg gcgaccagcg tgcgttcgtt ctttctgttg 11280 ttgtatctag tatgatgagg cgcaccgtgc taggcggatc ggtggcgtat ccggagggtc 11340 ctcctccttc gtacgaaagc gtgatgcagc aggtggcggc ggcggcgatg caacccccct 11400 tggaggctcc ttacgtgccc ccgcggtacc tggcacctac cgaggggaga aacagcattc 11460 gttattcgga actcacaccc ttgtatgaca ccacccggtt gtacctggtg gacaacaaat 11520 cggcggacat tgcctcgttg aactatcaga acgaccacag caacttcttg acaacggtgg 11580 tgcagaacaa tgactttacc cccacggagg ccagcaccca gaccatcaac tttgacgagc 11640 gctcccggtg gggcggtcag ctgaagacca tcatgcacac caacatgccc aacgtgaacg 11700 agttcatgtt tagcaacaag ttcagggcta gggtgatggt gtccagaacc acacctaaag 11760 aggtgacagt cacaacagac tatgatggta gtcaggacat cttggaatac gagtgggttg 11820 actttgagtt accagaaggc aacttctctg ccaccatgac catagacctg atgaataatg 11880 caattgttga taattaccta aaagtgggta gacagaatgg ggtactggag agtgacatag 11940 gtgttaagtt tgacactagg aactttaggc ttggttggga cccagtgaca gagttggtca 12000 tgcctggggt ctacaccaat gaagctttcc atcctgacat agtcctacta cctggctgcg 12060 gagtggactt cactgagagc cgcctcagta atctgctagg cattagaaag aaacagccat 12120 tccaggaagg gttccagatc atgtatgagg atctggaggg tggtaacatc cccgccctgc 12180 ttgatgtaaa tgcatatgag aagagcaagg aagataatac aaccaccaca aatgaagctg 12240 tggccgcggc ttcatctact gaagccaaag ctgtggtaga tgcttccact tcaacagaaa 12300 acaccactga tgaaaaagtc accaggggag atacatttgc cacccctgaa caagagaagg 12360 cagctgaggc agagtctgat attatgcttc tgtccaccga tgaaaacgaa actaaaaaac 12420 aactggttat tcgagcggtg accaaggata gtaaggacag gagttataat gtattgtcag 12480 atggaaagaa cacagcttac cgtagttggt acctggcata caattatggc gaccgtgaga 12540 aaggggtgcg ttcttggaca ctgcttacca cctcggatgt cacctgcggc gtggagcaag 12600 tctattggtc gctaccagat atgatgcaag atccagtcac ctttcgctcc acacgccaag 12660 ttagcaacta cccagtggtg ggcgcagagc tgctcccagt gcattccaga agcttctaca 12720 acgagcaagc cgtctactcg caacagctcc gccagtacac ctcgctcacg cacgtcttca 12780 accgcttccc cgagaatcag atcctcgtcc gcccgcccgc gccaaccatt accaccgtca 12840 gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gctgcgcagc agtatccggg 12900 gagtccagcg cgtgaccgtt actgacgcca gacgccgcac ctgtccatac gtatacaagg 12960 ccctgggcat agtcgcgccg cgcgtccttt caagccgcac tttctaaaaa aatgtccatt 13020 ctcatctcgc ccagtaataa caccggttgg ggcctgcgca cacctagcaa gatgtatgga 13080 ggcgctcgca gacgctccac tcagcaccct gtgcgcgtgc gcgggcattt ccgcgctccc 13140 tggggcgccc tcaagggacg ctctcgtact aggaccaccg ttgacgatgt gatcgaccag 13200 gtggtcgccg atgcacgtaa ctataccccc gcagccgcac ctgcatccac cgtggatgcg 13260 gtcattgaca gcgtggtagc cgatgcgcgc gcctatgctc gcgccaagag caggaggcgg 13320 cgtattgcca ggcgtcaccg agctactcca gccatgcgag ctgcaagagc tttattgcgg 13380 agagccagac gtgtggggcg aagagccatg cgtagagcgg ccagacgcgc ggcttcaggt 13440 gccagcgcag gcagggtccg caggcgcgcg gctacggcgg cagcggcggc catcgctagc 13500 atgaccaaac cacgaagagg caatgtgtat tgggtgcgcg acgccgccac cggccagcgc 13560 gtgcccgtgc gcacacgccc ccctcgcact tagaagatac tgagcagtct ccgatgttgt 13620 gtcccagcgg cgagatgtcc aagcgcaaat tcaaggaaga gatgctccag gtcatcgcgc 13680 ctgagatcta cggtcctgcg gtgaaggatg aaaaaaagcc ccgcaagatc aagcgggtca 13740 aaaaggacaa aaaggaagaa gatggtgatg atgggctggt ggagtttgtg cgcgagtttg 13800 ccccaaggag gcgcgtgcag tggcgcgggc gcaaagtgtg gccggtgttg agaccgggga 13860 ccacagtggt ctttacgcca ggcgagcgct ccagcaccgt ttccaaacgc tcttatgatg 13920 aggtgtacgg ggacgatgat attctcgagc aggcggctga tcgccttggc gagtttgcat 13980 atggcaaacg cagccgctcg ggagccaagg aagaggcatt gaccatcccc ttggatcatg 14040 gaaatcccac cccaagcctc aaacccgtga ccctgcaaca agtgctgccc acgccgccac 14100 gcaagggcat caagcgcgag ggcgaggatc tgtatcccac catgcagctg atggtgccca 14160 agcgccagaa gctggaagac gtgctggaga aaatgaaagt ggatcctgaa atccagcctg 14220 aagtcaaagt gaggccaatc aagcaggtgg cgcccggttt gggggtacaa accgtggata 14280 tcaagatccc caccgagtcc atggaaattc aaaccgaacc catgaagccc acctccagca 14340 ccattgaggt gcagacggat ccttggatgc ccgcgcctgc tcctgttacc actactactc 14400 gaagacctag aagaaagtat ggttcagcca acctgataat gccaaactat gctctgcatc 14460 catcaatcat acccactcct ggctaccgcg gcactcgcta ctaccgcagt cacagcaccc 14520 gccgacgtaa agcacctgcc acccgccgcc gtcgccgccg ccgtgccact agcaaactta 14580 ccccctcggc tatggtgcgg agagtgtacc gtgatgggcg cgcagctcct ctgacactgc 14640 cgcgcgcgcg ctaccatcct agcattgcca tttaacaact ctgcctcctt gcagatatgg 14700 ccctcacttg ccgccttcgt attcctattg ctggctaccg cggaagaaag tcgcgccgta 14760 gaagagcagg gttgtctggg agcgggatgc gtcgccaccg gcggcggcgc gccatcagca 14820 aacggttggg gggtggattt cttcccgctt tgattcccat catcgccgcg gcgatcggcg 14880 cgataccagg catagcttcc gtggcggtgc aggcctcgca gcgccactga cattggaaaa 14940 agatatctta taaataaaaa tagaatggac tctgacgctc ctggtcctgt gatatgtttt 15000 tgtagacgag atggaagaca tcaatttttc atccctggct ccgcgacacg gcacgcggcc 15060 gtatatgggc acctggagcg acatcggcaa cagccaactg aacgggggag ccttcaattg 15120 gagcagtcta tggagcgggc ttaaaaattt tgggtccact ataaagactt atgggaacaa 15180 agcttggaac agcagcacag ggcatgcgct gagacaaaag cttaaagatc agaatttcca 15240 acagaaggtg gtcgatggta tcgcctctgg aatcaatggg gtggtagatc tggccaacca 15300 ggccgtgcag aaacagatta acagtcgcct ggacccggct cccccagctc ctattcatga 15360 gttaatgcaa gtggaggaag agctcccttc attggaaaag cggggcgata agcgacctcg 15420 tccagatatg gaggaaacgc tgctgaccaa ggtggatgag ccgccctcct atgaagaggc 15480 tgtaaaactg ggaatgccca ctacaaagcc cattatgcct ctggccactg gagtgatgaa 15540 gccatctcag tctaaacctg cagttgctgc tacattggac ttgcccgctc ccgtggccac 15600 ccccaaacct gtcgccgccc cgaagcccac cgccgtgcaa cccgtggccg tggccagacc 15660 gcgtcccggt ggtcggccga atgcaaactg gcagagcact ctgaacagca tcgtgggttt 15720 gggagtgcac agtgtgaagc gccgtcgctg ctattgatta aatatggagt agcgcttaac 15780 ttgcttgtct gtgtgtgtat atgtcgatgc cgcccgccgt gctacagcaa agagagaagg 15840 agaagaggcg ccgctgagtt cctttcaaga tggccacccc atcgatgctg ccccagtggg 15900 cgtacatgca catcgccgga caggacgctt cggagtacct gagtccgggt ctggtgcagt 15960 tcgcccgcgc cacagatacc tacttcaatc tggggaacaa gtttaggaac cctaccgtgg 16020 ctcccaccca cgatgtgacc accgaccgta gccagcgcct gacgctgcgc tttgtgcccg 16080 ttgaccggga ggacaatacc tactcctaca aagtcagata caccctggct gtgggagaca 16140 acagggtgtt ggatatggcc agcacctact ttgacatcag gggcgtgttg gacagaggac 16200 ctagcttcaa accatactct ggcactgcct acaactccct ggctccaaaa ggagctccaa 16260 actccagtca gtggcaacaa aaggaaaaca atggtcaagg tgatgcaaag actcacacct 16320 atggtgtagc tgccactgga ggtattgaca ttgacaaaaa tggtcttcaa attggaatcg 16380 atgaaactaa agaagataat aacgaaattt atgcagacaa aacattccaa cctgaacctc 16440 aaattggaga agaaaactgg caagatagcg aaaactatta tggaggcagg gctcttaaac 16500 cggaaaccaa gatgaagcct tgctatggtt ccttcgctag accaactaat gcaaagggag 16560 gtcaagccaa aattaaacca gctcaagagg gtcaacagtc tatagattat gacatagacc 16620 tggctttctt tgatattcca agcactggcg gaggcaatgg cacaaatgta aatgacaagc 16680 cagatatggt tatgtatact gaaaatgtaa atctggaaac tccagacact catcttgttt 16740 acaagccagg aacttcagat gacagttccg aggccaattt aactcagcaa gccatggcta 16800 acagacccaa ctatattggg tttagagata actttattgg cgtcatgtac tacaacagca 16860 ctggcaacat gggagtgctt gctggtcaag catcccagct aaatgctgtg gtggacctgc 16920 aagacagaaa caccgagctg tcttatcagc tattacttga ctctctgggc gacagaacca 16980 ggtattttag tatgtggaat caggcggtgg acagctatga tcctgatgtg cgcattattg 17040 aaaaccatgg tgtggaagat gaattgccaa actattgctt cccattggac ggagctggca 17100 ctaatgctgt ttaccaagga gttaagacaa aagaggataa taatggcgaa tgggaaacag 17160 acacaaatgt tgcatcgcag aatcagatat gcaagggcaa catatatgct atggagatca 17220 acctgcaagc caacctgtgg aaaagtttcc tttactccaa cgtggctctg tacctaccag 17280 actcctacaa gtacactcca tccaacgtga cactccctac caacactaac acctatgact 17340 acatgaatgg cagggtggtg tctccatccc tggtggatgc ctacattaac attggcgcca 17400 ggtggtctct ggatgccatg gacaatgtca accctttcaa ccaccaccgc aatgccggcc 17460 tgcgctaccg gtccatgctt ctgggcaacg gccgatacgt gcccttccac atccaagtgc 17520 cccagaaatt cttcgctatc aagaacctgc tgcttctccc aggctcatac acctacgagt 17580 ggaacttccg caaggatgtc aacatgatcc tgcagagttc ccttggcaat gacctcagaa 17640 ccgatggggc caccatccag tacaccagca tcaatctcta tgccaccttc ttccccatgg 17700 ctcacaacac tgcctccacc ctggaagcca tgctgcgcaa tgacaccaat gaccagtcct 17760 tcaatgacta cctctcagct gccaacatgc tttaccccat ccctgccaat gccaccaacg 17820 tgcccatctc catcccatct cgtaactggg ctgccttcag gggctggtct ttcacccgcc 17880 tcaagaccaa ggagacccca tctctgggat cagggttcga tccctacttc gtctactcag 17940 gctccattcc atacctggat ggaactttct accttaacca cactttcaag aaagtctcca 18000 tcatgtttga ctcttctgtc agctggccag gcaatgacag gctgctgact cccaatgagt 18060 tcgaaatcaa gcgcactgtt gatggggaag ggtacaatgt ggcacaatgc aacatgacca 18120 aagactggtt cctggttcag atgctctccc actacaacat tggctaccag ggcttctaca 18180 tcccagaagg atacaaggac cgcatgtact ccttcttcag aaacttccag cccatgagcc 18240 gccaggtggt cgatcaggtc aactacaaag actacatggc agtcaccctt gcctatcagc 18300 acaacaactc tggctttgtg ggctacctcg cgcccaccat gcgacagggc caaccctacc 18360 ctgctaacta cccatacccg ctcattggca agactgcagt caacagtgtc acccagaaaa 18420 agttcctctg cgacagggtc atgtggcgca tccccttctc cagcaacttc atgtccatgg 18480 gggcccttac cgacctgggg caaaacatgc tttatgccaa ctccgcccac gcgctagaca 18540 tgaatttcga agtagacccc atggatgagt ccacccttct ctatgttgtc ttcgaagtct 18600 tcgacgtggt cagagtgcac cagccccacc gcggcgtcat cgaagctgtc tacctgcgca 18660 cccccttctc agctggtaac gccaccacat aagcgccttg cttcttgcaa gtggctgcag 18720 cagcatggcc tgtggatcct ccactggatc caatgagcaa gagctcaggg ccatcgccat 18780 agacctgggc tgtggaccct atttcctggg aacctttgac aagcggtttc caggcttcat 18840 ggctcctgac aagctcgcct gtgccattgt caacacggca gggcgcgaga ctggtggtga 18900 gcactggctg gcttttggat ggaacccccg ctccaatacc tgctatctct ttgacccgtt 18960 tgggttttca gacgagcgcc tcaagcagat ctatcaattc gagtacgagg ggctcctgcg 19020 ccgcagtgcc ctggctacta aggaccgatg catcactctg gaaaagtcta cccagaccgt 19080 gcagggtccg cgctcggctg cctgcgggct cttctgctgc atgttcctcc atgcttttgt 19140 gcactggccc gaccgcccca tggacaacaa ccccaccatg aatttgctga cgggggtacc 19200 caacaacatg ctccaatcgc cccaagtaga gcccaccctg cgccacaacc aggaggcact 19260 ctatcgcttc ctgaactccc actcatctta ctttcgttct aaccgcgcgc gcattgagaa 19320 ggccactgcc ttcgatcgaa tgaataataa catgtaaacc aaattgtgtg tggctcaaat 19380 aaacagcact ttattgttta catgcactga ggctctggga tgatcatttt ttaaaaatcg 19440 aaggggttct ggcgggaatc agcatggcca gatggcaggg acacgttgcg gaactggaac 19500 ttgttctgcc acttgaactc gggaatcacc agcctgggaa ctggaatctc tggaaaggta 19560 tcttgccata gctttctggt cagttgcaga gcgccaagca ggtcaggagc agatatcttg 19620 aaatcacagt tggggccaga attctgggcg cgggagttgc ggtacactgg gttgcagcac 19680 tggaacacca taagggcagg gtgtctcacg ctcgccagca cggtctcgtc actgatgcaa 19740 gacacatcca ggtcttcagc attggccatt ccaaaggggg tcatcttgca ggtctgtctg 19800 cccatcacgg gagcgcagcc aggtttgtgg ttgcaatcac aatgaagggg gatcagcatc 19860 atcttggcct ggtcgggggt aatccctggg taaacagcct tcatgaaggc ttcatactgc 19920 ttgaaagctt cctgggcttt ggttccctcg gtgtagaaca ctccacaaga cttgctggaa 19980 aactgattag tagcgcagtt ggcatcattc acacagcagc gggcgtcgtt attagccagc 20040 tggaccacat tcctgcccca gcggttctgg gtgatcttgg ctcgatctgg gttctccttc 20100 aacgcgcgct ggccgttctc gctcgccaca tccatctcaa tgacatgttc cttctggatc 20160 atgatgttgc catgcaggca tctaatcttg ccttcataat cagtgcagcc atgaggccac 20220 agcgcgcacc cggtgcactc ccaattgtta tgggggatct gggaatggct atgaaccagc 20280 ccttgcagga atcttcccat catcacagcc agggtcttta tgctggtaaa ggtcagcggg 20340 ataccgcggt gctcctcgtt cacatactgc tggcagatgc gtctgtagtg ctcggcctgc 20400 tcgggcatca gcttgaaaga ggttttcaac tcattatcca gcctgtatct ctccatcatg 20460 atggacatta cttccatgcc cttctcccag gcagaaacaa tagggagact caggggattc 20520 ttgacagtag agacaacctt acttaagggg tcatcactgc caatcttttc gatgcttctc 20580 ttgccatcct tctcggtgat gcgcaccggc gggtagctga atcccacagc caccaactga 20640 gcctcttccc tttcgtcttc gctgtcttga ctgatgtctt gcagaggaac atgtttggtt 20700 ttcctgggtt tcttcttggg cggcagctct ggaggactct ggctccgttc cggagacccc 20760 atggatgagc gagagttgtc gctcaccact tggatctggc tgcctgtaga agaactggac 20820 cccacgcggc ggtaggtgtt cctcttggta ggcagaggtg gaggcgacgg gctccggtcc 20880 ggtctgggtg gcggatggct ggcggagccc cttccgcgtt cgggggtgcg ctccagatgg 20940 cggtcgtctg actgacctcc gcggctggcc attgtgttct cctaggtaga gaaacaagac 21000 atggagactc agccatcgct gccatcgcca tccaccacca caagcaccgc cgaggaggag 21060 gagtgtttaa ccaccccacc atgcagcccc gctaccacca ccagcaccct tgaaagcgag 21120 gtcgacacgg tcgtggagga tttacaggct atggaagata ttgaggcagc tgtcgagcaa 21180 gaccccggct atgtgacacc ggcggagcat gatgaggatc tagcgcgctt tctcgacggt 21240 gtggagaaag cgaaacaaga tgaggacgag gaagaggcag aagcacaacc atcggtggcc 21300 gactacctca ccggcctagg gctagaagac gtgctgctta agcatcttgc aaggcagaca 21360 gtcatagtca aagacgccct gctagagcgc tccgaggtgc cactcagtgt ggaagacctc 21420 agtcgcgcct atgagctaaa cctcttctcg cctcgcaagc cccccaagcg tcagcccaac 21480 gggacctgtg agcccaatcc gcgcctcaac ttctatccag ccttcactgt gcccgaagta 21540 ctagctacct accacatctt tttcaagaac caaaagatcc ccatctcctg ccgcgccaat 21600 cgcacccgcg cagatgccct actcaacttg gggcccggcg ctcgcatacc tgatatcgct 21660 tccttggaag aggttcctaa gatctttgag ggtctgggca atgaggaaac tcgggcagca 21720 aacgctctgc aaagagaaac agatgatggt gaacaccaca gcgctctggt ggagctccag 21780 ggcgacaacg ctcgtcttgc agtcctcaaa cgcagcatcg aggtcaccca tttcgcctac 21840 cccgcactta atctcccacc caaagtcatg agctcggtca tggacacgtt gctcatgaag 21900 cgcgcgagcc ccatctccga ggatcagaac atgcaggacc ccgatgcctc agatgaaggc 21960 aagcctgtag tcagcgacga gcaactggct cgctggctag gctctgactc cccccagtct 22020 ttggaggagc ggcgcaagct tatgatggca gtggtcctga tcacagcgga gctggagtgt 22080 ctccgccgct tcttcactga cccagagacc ctgcgcaagc ttgaggagaa cctgcattac 22140 acattcagtc atgggttcgt gcgccaggcg tgcaagatct ccaacgttca actcaccaac 22200 ctggtctcct acctgggcat cttgcatgaa aaccggctgg ggcagaacgt gctccacacc 22260 accctgaagg gggaggcccg ccgcgactat atccgcgact gtatctacct ctacctatgc 22320 tacacctggc aaagcgggat gggtgtgtgg caacagtgct tggaagagca aaatctaaaa 22380 gagctggaaa agctgcttca gaaatctctt aaatctctgt ggaccgggtt cgatgagcgg 22440 accaccgctt cggacatggc cgatattatc ttccccgagc ggctcagaca cactctgcgc 22500 gacgggctgc ctgactttgc cagccagagc atgctacaaa actttaggtc attcatcttg 22560 gaacgctccg ggatcctgcc cgccacttgc tgcgcactgc cctccgattt tgtgcccatc 22620 acctaccggg agtgcccccc gccgctatgg agccactgct acctgttccg cctggccaac 22680 tacttggcct accactctga tgtgatagaa gatgttagtg gcgaagggct cctggagtgc 22740 cactgccgct gcaacctctg caccccccac cgctccctcg cctgcaatcc ccagctgctg 22800 agcgaaaccc agatcatcgg caccttcgag ttgcaaggtc ccagcggcga aggcgagggg 22860 tcctctccgg ggcaaagtct gaaactgact ccggggctat ggacctccgc ttaccttcgc 22920 aagttcgccc ccaaagacta ccacccctat gagatcaggt tttatgaaga ccaatcacag 22980 ccccccaagg ccgaactgac ggcctgcgtc atcacccagg gggcaatctt ggcccaattg 23040 caagccatcc aaaaatcccg ccaagaattt ttgctgaaaa agggacacgg gatctatcta 23100 gacccccaga ccggtgagga gctgaataca cgcttccctc aggatgcccc gaggaggcaa 23160 gagaatgaaa gttcagatgc cgcccgagga ggagctggaa gactgggaca gtcaggcaga 23220 ggaggaagac tgggacagcc aggcagaaga ggaggacagc ctggaggagg acagtctgga 23280 ggaaggcgag gagcccaagg aagaggcagc cgccgccaga ccatcgtcct cggcggtgga 23340 gacaagcaag gtcccagaca gcacggctac cacctccgct ccagctcaag gggccgctcg 23400 gcgacccaac agtagatggg acgagacggg tcgcttccag aaccccacca ccgtcaagac 23460 cggtaagcag gagcggcagg gatacaagtc ctggcggggg cataaaagtg ccatcatcgc 23520 ttgcttgcag gagtgtgggg gcaatatatc ctttgccaga cgctacctgc tattccatca 23580 cggggtgaat ttcccccgca acatcttgca ttactaccgt cacctccaca gcccctacta 23640 ccagcagcaa gagacagcag aggaaaccag cggcaactcc gagagttaga aaaccagcag 23700 ctaaaaaatc cacagcggcg gcagcaggtg caggcggact gaggatcacc gcgaacgagc 23760 cagctcagac cagggagttg aggaatcgga tctttcccac cctctatgcc atattccaac 23820 aaagtcgggg tcaggaacaa gaactgaaag taaaaaacag atctcttcgc tcgctcaccc 23880 gcagttgttt gtatcacaag agcgaagacc aacttcagcg cactctcgag gacgccgagg 23940 ctctcttcaa caagtactgc gcgctgactc ttaaagagta gactgcgcgc gcttggcgag 24000 aaaaggcggg aattacgtca cctcttggcc acacctgtgc ttcattatga gtaaagaaat 24060 tcccacgcct tacatgtgga gctatcagcc ccagatggga ttggccgctg gcgccgccca 24120 ggactactcc acccgcatga attggctcag cgccggtccc gcgatgatct cacgggttaa 24180 tggtgtgaga gagcaccgaa accagatact cctagaacag tccgccctca ccgccactcc 24240 ccgcaatcac ctcaaccccc gtaattggcc cgccgccctg gtgtaccagg aaactcctgc 24300 tcccactaca gtactacttc ctcgtgacgc ccaggccgaa gttcagatga ctaactcagg 24360 tgtacagctg gcgggtggtg ccaccctgtg tcgtcaccgg ccaagaccgg gtataaaggg 24420 cctggtgatc agaggccgag gtattcagct caacgacgag tcggtgaact cttcgcttgg 24480 tctgcgacca gacggcatct tccaaatagc tggttgtggg agatcttcct tcactcctcg 24540 tcaggctgtc ctgactttgg agagttcgtc ctcgcagccc cgctcgggcg gcatcgggac 24600 tctccagttc gtggaggagt ttactccctc ggtctacttc aaccccttct ccggttctcc 24660 tgggctttac ccggacgagt tcatcccgaa ctacgacgcc atcagtgaag cggtcgacgg 24720 ctacgattaa tgtctaatgg tggcgcggct gagctagctc gactgcgaca cctagaccac 24780 tgccggcgct ttcgctgctt tgctcgggat ctctgcgagt tcatctactt cgagtaccct 24840 gacgaacatc ctcagggacc tgcccacgga gttcggatta ccattgaagg ggctatcgat 24900 tctcacctgc ttcggatctt caccgctcgg ccagtgctag ttgagcgcaa ccagggcgac 24960 accaccatct ccctctgctg catttgtgac aaccccggat tgcatgaaag cttttgttgt 25020 cttctttgta ctgagtataa taaaagctga aattagagac tactccggac tctcttgtcg 25080 tctgaacaac accaaccaga cccttcactt cagcgggaac cagactactc ttcactgtaa 25140 ggcttataac tataagtatc ttacttggat atacaaagga acaccgtttg ctgtggtaaa 25200 caggtgctcc aacgacggtg ttctcctcac cttcctaggc aacttctcca actttacctt 25260 ttctgttcgc agaaacaagc ttaccctcct tcagccctac tttcctggga tctatacctg 25320 cctcagtgga ccttgcaacc acacttttca cctgattgaa aactctaccc ttaccttccc 25380 agcgccaatc cctactaaca gctcggagtc caactcttcc attaccgctg atactaacac 25440 tcctaaaacc ggaggtgagc tccgcagcct tcccccggct gcagataacc cttgggtggt 25500 agcgggattt gtagcgctag gaatagttgc gggtgggctc gcgttcgtcc tctgctacct 25560 ataccttacc tgctgctcat atttagtagt actgtgctgt tggtttagaa aatgggggcg 25620 ctactaatca cacttgcttt actttcgctt ttgggtctga gctcggctaa tagcgagaaa 25680 ccaagctgtc tagaaacaaa ctctccagac tgtgtggttc ctcatgggct ctcagaccca 25740 gctgatgatc catgcttaac ttttgaccca gaaaaaaact gctcggtgac tatgcagccc 25800 tatgcttaca tgtgcacatc tgttataaag tgcggatggg gctgtaaacc gattgaaatt 25860 acccacaaag gcaaaacctg gaataatagt ttgtttaaca catggcagcc tggagacgag 25920 cagtggtata cggccggcca ctggtggaga tgactgaccc catggaaaac tcctctgcca 25980 acgacctgga catggacggc cgttcatctg agcagcgact ggtccagatg cgcattcgcc 26040 agaagcagga acgcgccgcc agagagctca aggatgccat tgaaattcac ctgtgcaaga 26100 agggcatctt ttgcttggtt aagcaagcaa agatttctta tgaaatcact gacaacgacc 26160 accgcctgta ttatgagctc ggtccacagc ggcagaaatt cacctgcatg gttggagtca 26220 accccatagt catcactcag caggctgcag aaattaaagg gtgcatccac tgttcctgtg 26280 attcccaaga atgcgtccac accatagtca agaccctctg cggccttcga gatcttcttc 26340 caatgaacta accccttccc ccaacccaat aaaacattgg ttttaatcat aataaaaaat 26400 cacttacttt aaatctgaaa cagtgtctcc gtccaagttt tcttgtagca ccacttcact 26460 cccctcttcc cagctctggt actgcaagcc ccggtgggct gcaaactttc tccacacctt 26520 aaaagggatg tcaaattcct cttgtccaac aatcttcatt gtctcttcct agatgtccac 26580 aaagcgcgcg cgggtggaag atgactttga ccctgtctac ccatacgatg ctgagctggc 26640 accgtctgta cccttcatcg cccctccctt cgtttcgtca gacggatttc aagaaaaacc 26700 cctgggagtt ctgtccctaa gactagccaa cccagtcact actaaaaatg gggaactcac 26760 acttaaactg ggagatgggg tgggcataga ctcagatgga aacctcacag cacagacagt 26820 tactaaagca acatcccccc ttactgtttc caataacgca attgcactta acatggacaa 26880 acctttttac agtagcaatg gaaaactatc cttacaagtt acatcaccat taaagatagt 26940 cgactcttta aatacattgg ctattggcta tgggcaaggc ttaggactaa acaatagtgc 27000 tcttgctgtg caattagcat ctccccttac atttgacagc aacagcaaaa ttaaaataaa 27060 tttgggaagc gggccattaa aaattaatgc gaataaactg tcaattaact gcctaagggg 27120 tgtatatgta acaactgacg gaacttccat tgaaacaaat ataagctggg caaaaggaat 27180 gaggtttgaa ggtaatgcca tggctgtaaa cgttgacagc accaaaggtc tacaatttgg 27240 cactaccagc acagaatcag gagtcactaa cgctttccct atccagttaa agattggatc 27300 tggtcttagt tttgacagca caggagcact tgtagcttgg gataaggata atgacaagct 27360 tacactgtgg acaaccgctg acccatcacc taattgtacc atatatacag acaaggatgc 27420 taaacttaca ctttgtctta caaaatgtgg cagtcaaata ctaggcagtg tttcagtact 27480 ggctgttaaa gctggaaccc tacagccaat cagtgaaaaa ataggtactg ctttggtttc 27540 actaaaattt aataacaacg gtgtattgtt aagcaactcc acattaagta atgaatactg 27600 gaactacagg aagggagatg tcacaccagc cgaagcctat actaatgctg tgggttttat 27660 gccaaacatc aaggcatatc ctaaaaacac aaactctgcc tcaaaaagcc acattgtagg 27720 acaagtgtac cttaatggag atgaaactaa accaatgcat ttaatcatta catttaatga 27780 aaccagtgat gaaacatgca catattccat aacgttccaa tggaaatgga acattggaac 27840 atacaccagc gacacccttg caacaagctc ctttaccttt tcttacattg cccaagaata 27900 aaaactgcag acaacaataa agtttaaatg ttttatttaa acagtttcac agaaccctag 27960 tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 28020 ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 28080 tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 28140 agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 28200 cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 28260 tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 28320 aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 28380 gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 28440 agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 28500 tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 28560 gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 28620 cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 28680 agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 28740 agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 28800 cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 28860 ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 28920 ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 28980 cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 29040 gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 29100 acgtagtcat tctcgtactt tgagtggcaa aaccttgctc tcgaacagca cacgtctcgt 29160 cgcctcctgt cccttctctt cgccttttca gtgtgatagt tgtaatacag ccattcacga 29220 agctcagtca gaagatcttc agcgtctgtt gtcaaaaaca atccatccaa tctgattgct 29280 ttcaaaacat cacaaacagt cgaataagcc aaacccatcc aggcaatgca attattttgg 29340 ttatccacaa tgggaggggg cggaagacat ggaagaggca taattaattt tttaatccaa 29400 tcgatcacgc agcacttcaa aatgaagatc gcgaaggtga cacctttcac ccccactgtg 29460 ttgatgaaaa ataacagcca agtcaaaatt gatgcggttt tcaaggtgct cgactgtagc 29520 atcaagcaga gcttccacac gcacgtccac aaataacaga atagcaaaag cgggaggagg 29580 aagtaaatcc tcaatcatca tagtacagtc catcaccatc cctaaataat tttcatcctt 29640 ccagccttgg actatatttt taaactgctc ttgtaaatcc aaaccacaca tgtggaaaag 29700 ttcccaaaga gctccctcaa ctaccattct taagcacacc ttcatagtga caaaatatct 29760 tgttcctctg tcacctgcag caaattacaa agtccaatat taggatctat gcccagagat 29820 ctaagctcat ccctcaattc caactgtaaa aaggcttcca gatctgccct aacttgttca 29880 gccagtgggc tccctggaat aagcgtggga gaagccaaac tgcaaaacag acgcatgccg 29940 ccataattac caccagaaaa cactacgtta cagtatgcat gctgattcat tccagtaatt 30000 tcatccagtg tattggatac aaaaaaaggc aagcactctc tcactaattg tattatggag 30060 acattatcac acaggtaaca atttaaaggt tgtggaacaa taatgcagta agtaaccacg 30120 gtgcgctcca acatggttag taatttttag ttctgaaaaa caaaacatac aaaaaattat 30180 atcatactca tttggcgaac tggtggaaaa atgaccctat ctagcacaag gcaagccact 30240 ggatcaccaa tgcgcccctc ataaaacctg tcatcatgat taaaaagcaa caccgaaagc 30300 tcttccctat gtcctgcatg aatgattcta gctgaggaat ataagccagc gcaattagta 30360 tctgttaaag aaaaaaaacg gccaacatag cctctaggaa ttagcacact taatcttaaa 30420 gacattactg ccatccccct tggatttaag gtaaaattta caggagcata gaaaatatac 30480 tgatttccct cctgcacagg cagcatagca ccaggtccct ctaaaaacac acacaaagct 30540 tctgcagcca tagcttaccg cgcaaaccag gcacagcagt gagctaaaag gacaaagctc 30600 taactcacta gccaacctgg cgcacaatat atagttagtc cttacactga cgtaaccgac 30660 caaagtctaa aaaccccgcc aaaaatacac acacgcccaa aaaacgcccc gtgagtcaaa 30720 aaacagtttc acttcctcgt tacacccaaa acgtcgtcac ttccggattc ccacggttcg 30780 tcacttccgg agctccttgc ttaattaacc ccgcccaaaa cgtcatcgtc cgcgtcacgc 30840 cgccccgccc cgcgaccgtt gaccccgggc caatcaccgc acatcccgca aaattcaaac 30900 tcgtctaatt tgcatattgg cacactgccc atataaggta tattattgat gatgatttaa 30960 atcatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 31020 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 31080 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 31140 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 31200 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 31260 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 31320 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 31380 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 31440 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 31500 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 31560 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 31620 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 31680 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 31740 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 31800 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 31860 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 31920 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 31980 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 32040 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 32100 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 32160 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 32220 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag 32280 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 32340 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 32400 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 32460 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 32520 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 32580 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 32640 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 32700 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 32760 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 32820 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 32880 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 32940 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 33000 gtatcacgag gccctttcgt cttcaagaat tgatttaaat 33040 <210> 9 <211> 32701 <212> DNA <213> Artificial Sequence <220> <223> pBLY6.dE1.dE3.5IXP <400> 9 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg gtactgaaat gtgtgggcgt 480 ggcttaaggg tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt 540 gcagcagccg ccgccgccat gagtgggagc gcttcctttg aagggggcgt ctttagccct 600 tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat ggggtctaca 660 gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc tactatgact 720 tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa cactgttcgg 780 gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac taccccaaat 840 aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct ggagtctttg 900 acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca gactcaagct 960 gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat aaaccgagac 1020 tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat atgccctgga 1080 ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat agaggtggga 1140 ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc tccactgcag 1200 agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc gttgggcatg 1260 atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct tggtgtaagt 1320 gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt gcattttgga 1380 ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca tgttatgcaa 1440 gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt tggaggggaa 1500 agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc actcatccat 1560 aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg gatctgacac 1620 atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact tggggcggag 1680 ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc cttcacatat 1740 ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg gagcgatgaa 1800 gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc taagcagctg 1860 tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca gatggtagtt 1920 tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca tcatttccct 1980 cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca gggaaaggag 2040 ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg gcattttgga 2100 gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt ctatggcatc 2160 tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta tggaaccagg 2220 cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt ccgagtcagg 2280 gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag ggtgcgcttc 2340 aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc ggccaggtag 2400 caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc gcgcagctta 2460 cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata gagttttggg 2520 gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca gacggtttcg 2580 cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt tccgccatgt 2640 tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg ggtgacaaag 2700 aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg cgtgccgcgg 2760 tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca ggccagcaca 2820 aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac tttttccaag 2880 gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt gtaagtgtat 2940 gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct ctgctcgtcc 3000 tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta ttccctctcg 3060 aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga ggatttgata 3120 ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc agaaaagaca 3180 atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga aagcagtttg 3240 gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc ggctatgttg 3300 agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt taattcatct 3360 gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac actggttgcc 3420 acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg agaacagaaa 3480 gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt gaaaatccca 3540 ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga catttgccat 3600 tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg catgggatgg 3660 gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc ttccagaatc 3720 cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata atcatagagt 3780 tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt ttctgctctg 3840 tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct ttggaagatg 3900 ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata ggagtcttgc 3960 agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc gagggtctct 4020 ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt gagaaggtat 4080 tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga acggtaagaa 4140 cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc cacggggagt 4200 gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt gtccctgacc 4260 atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg ctcccacagt 4320 tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac atcgttgaat 4380 aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg aggcacttct 4440 gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt gatgttgtga 4500 cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag ttttttaagc 4560 tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc ccagtcaggc 4620 aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc tgtttgtaag 4680 cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt aacacagtaa 4740 aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag gtcgtgggcg 4800 aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg gacaagctgc 4860 ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa gagcctttct 4920 gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt ggaggaatgg 4980 ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg cttgtgcttg 5040 tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat gagctgtacc 5100 tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg catctcgtgt 5160 tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt catgctgacg 5220 agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc gaggacgaga 5280 gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc agtagggaga 5340 gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag atgatatttg 5400 atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg cccctggggt 5460 gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg catggtaagg 5520 tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt tctggacctg 5580 gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg tactgcgccc 5640 tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga cgcctctggg 5700 tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa tcaatttcgg 5760 tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg tcttggtagg 5820 cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg cccgctcgtt 5880 ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat gcattcatgc 5940 ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg gcgcgcatga 6000 ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg cagagacgct 6060 ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac atgatccagc 6120 gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg gcttcgtaaa 6180 agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac tcctcctcca 6240 gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct cccgggatct 6300 cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact tcctcctcag 6360 gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc agacgatcga 6420 tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg gcgcgcccgt 6480 cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg tgactgggag 6540 ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc cctgtaggga 6600 ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc tgaacgaagg 6660 cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg cgggctgctg 6720 agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt ctgagacggc 6780 ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc aggcggtcgg 6840 ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct tgcatgagtc 6900 gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt ccgtaccctc 6960 tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg gcttgctgca 7020 cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag gcccccgtgt 7080 tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt cctggtcgta 7140 cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa tcgttgcagg 7200 ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg tagagaggcc 7260 atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg tggtatccgt 7320 agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc ggaaactctc 7380 gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc acggtctggc 7440 ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa gcgttgagcg 7500 gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac cctggttcga 7560 gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta ttggcaatcc 7620 cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg tttttcaggg 7680 catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt ggctcgcgcc 7740 cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt cgagcctgca 7800 gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg tttctaagac 7860 cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt tttgtttttg 7920 ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc agcagcaggc 7980 tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg ctgtaagcgg 8040 ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg agggattggc 8100 aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa aggacgttcg 8160 cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg agcccgagga 8220 gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg atcgaaaacg 8280 ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc ccgctcgcgc 8340 tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg aggaaagcaa 8400 cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg aggtgaccct 8460 gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca ctagcaaacc 8520 cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg cattcagaga 8580 ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg tgaatatcct 8640 gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg cggcgatcaa 8700 ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa ccccttacgt 8760 gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc tgaaagtgct 8820 aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg cggtgagcgc 8880 cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac gagccctgac 8940 gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc attggctgcc 9000 tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag tagtagacga 9060 ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt tttgctagat 9120 ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt ccggcattaa 9180 ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga cccgcaaccc 9240 cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg aggccgtggt 9300 gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg ccctggtgga 9360 gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc tcgagcgcgt 9420 ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga ccgacgtgcg 9480 cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt ccatggtagc 9540 gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg aagactatac 9600 caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg aggtgtacca 9660 gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag tgaacttaag 9720 ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg gcgacagggc 9780 gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg tgtccccctt 9840 cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa acttgtatcg 9900 cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca ctaatgtgag 9960 tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact ttctgctgac 10020 caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg aacggatctt 10080 gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga cccctagcgc 10140 cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca acagaccatt 10200 cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg attacttcac 10260 caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg gcgagtatga 10320 catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg cgttctcgcc 10380 taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct cctccgtgct 10440 gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct ttcccagtct 10500 gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc gccctcgctt 10560 gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg aaaagaactt 10620 ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga cctatgccca 10680 ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca gacgccagcg 10740 ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg acagcagcgt 10800 gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc gtccccgtat 10860 tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat ggcgaccagc 10920 gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg ctaggcggat 10980 cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag caggtggcgg 11040 cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac ctggcaccta 11100 ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac accacccggt 11160 tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag aacgaccaca 11220 gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag gccagcaccc 11280 agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc atcatgcaca 11340 ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct agggtgatgg 11400 tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt agtcaggaca 11460 tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct gccaccatga 11520 ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt agacagaatg 11580 gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg cttggttggg 11640 acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc catcctgaca 11700 tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt aatctgctag 11760 gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag gatctggagg 11820 gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag gaagataata 11880 caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa gctgtggtag 11940 atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga gatacatttg 12000 ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt ctgtccaccg 12060 atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat agtaaggaca 12120 ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg tacctggcat 12180 acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc acctcggatg 12240 tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa gatccagtca 12300 cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag ctgctcccag 12360 tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc cgccagtaca 12420 cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc cgcccgcccg 12480 cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 12540 cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc agacgccgca 12600 cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt tcaagccgca 12660 ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg gggcctgcgc 12720 acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc tgtgcgcgtg 12780 cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac taggaccacc 12840 gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc cgcagccgca 12900 cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg cgcctatgct 12960 cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc agccatgcga 13020 gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat gcgtagagcg 13080 gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc ggctacggcg 13140 gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta ttgggtgcgc 13200 gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac ttagaagata 13260 ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa ttcaaggaag 13320 agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat gaaaaaaagc 13380 cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat gatgggctgg 13440 tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg cgcaaagtgt 13500 ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc tccagcaccg 13560 tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag caggcggctg 13620 atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag gaagaggcat 13680 tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg accctgcaac 13740 aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat ctgtatccca 13800 ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag aaaatgaaag 13860 tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg gcgcccggtt 13920 tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt caaaccgaac 13980 ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg cccgcgcctg 14040 ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc aacctgataa 14100 tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc ggcactcgct 14160 actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc cgtcgccgcc 14220 gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac cgtgatgggc 14280 gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc atttaacaac 14340 tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt gctggctacc 14400 gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg cgtcgccacc 14460 ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct ttgattccca 14520 tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg caggcctcgc 14580 agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga ctctgacgct 14640 cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt catccctggc 14700 tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca acagccaact 14760 gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt ttgggtccac 14820 tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc tgagacaaaa 14880 gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg gaatcaatgg 14940 ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc tggacccggc 15000 tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt cattggaaaa 15060 gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca aggtggatga 15120 gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc ccattatgcc 15180 tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg ctacattgga 15240 cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca ccgccgtgca 15300 acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact ggcagagcac 15360 tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct gctattgatt 15420 aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg ccgcccgccg 15480 tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag atggccaccc 15540 catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct tcggagtacc 15600 tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat ctggggaaca 15660 agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt agccagcgcc 15720 tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac aaagtcagat 15780 acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac tttgacatca 15840 ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc tacaactccc 15900 tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac aatggtcaag 15960 gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac attgacaaaa 16020 atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt tatgcagaca 16080 aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc gaaaactatt 16140 atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt tccttcgcta 16200 gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag ggtcaacagt 16260 ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc ggaggcaatg 16320 gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta aatctggaaa 16380 ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc gaggccaatt 16440 taactcagca agccatggct aacagaccca actatattgg gtttagagat aactttattg 16500 gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa gcatcccagc 16560 taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag ctattacttg 16620 actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg gacagctatg 16680 atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca aactattgct 16740 tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca aaagaggata 16800 ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata tgcaagggca 16860 acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc ctttactcca 16920 acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg acactcccta 16980 ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc ctggtggatg 17040 cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc aaccctttca 17100 accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac ggccgatacg 17160 tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg ctgcttctcc 17220 caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc ctgcagagtt 17280 cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc atcaatctct 17340 atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc atgctgcgca 17400 atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg ctttacccca 17460 tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg gctgccttca 17520 ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga tcagggttcg 17580 atccctactt cgtctactca ggctccattc catacctgga tggaactttc taccttaacc 17640 acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca ggcaatgaca 17700 ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa gggtacaatg 17760 tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc cactacaaca 17820 ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac tccttcttca 17880 gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa gactacatgg 17940 cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc gcgcccacca 18000 tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc aagactgcag 18060 tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc atccccttct 18120 ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg ctttatgcca 18180 actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag tccacccttc 18240 tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac cgcggcgtca 18300 tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca taagcgcctt 18360 gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat ccaatgagca 18420 agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg gaacctttga 18480 caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg tcaacacggc 18540 agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc gctccaatac 18600 ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga tctatcaatt 18660 cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat gcatcactct 18720 ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc tcttctgctg 18780 catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca accccaccat 18840 gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag agcccaccct 18900 gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt actttcgttc 18960 taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata acatgtaaac 19020 caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg aggctctggg 19080 atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc agatggcagg 19140 gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac cagcctggga 19200 actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag agcgccaagc 19260 aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc gcgggagttg 19320 cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac gctcgccagc 19380 acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat tccaaagggg 19440 gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg gttgcaatca 19500 caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg gtaaacagcc 19560 ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc ggtgtagaac 19620 actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt cacacagcag 19680 cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg ggtgatcttg 19740 gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac atccatctca 19800 atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt gccttcataa 19860 tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt atgggggatc 19920 tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc cagggtcttt 19980 atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg ctggcagatg 20040 cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa ctcattatcc 20100 agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca ggcagaaaca 20160 atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg gtcatcactg 20220 ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg cgggtagctg 20280 aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg actgatgtct 20340 tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc tggaggactc 20400 tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac ttggatctgg 20460 ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt aggcagaggt 20520 ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc ccttccgcgt 20580 tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc cattgtgttc 20640 tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc atccaccacc 20700 acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc cgctaccacc 20760 accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc tatggaagat 20820 attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca tgatgaggat 20880 ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga ggaagaggca 20940 gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga cgtgctgctt 21000 aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg ctccgaggtg 21060 ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc gcctcgcaag 21120 ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa cttctatcca 21180 gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa ccaaaagatc 21240 cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt ggggcccggc 21300 gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga gggtctgggc 21360 aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg tgaacaccac 21420 agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa acgcagcatc 21480 gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat gagctcggtc 21540 atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa catgcaggac 21600 cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc tcgctggcta 21660 ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc agtggtcctg 21720 atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac cctgcgcaag 21780 cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc gtgcaagatc 21840 tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga aaaccggctg 21900 gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta tatccgcgac 21960 tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg gcaacagtgc 22020 ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct taaatctctg 22080 tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat cttccccgag 22140 cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag catgctacaa 22200 aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg ctgcgcactg 22260 ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg gagccactgc 22320 tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga agatgttagt 22380 ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca ccgctccctc 22440 gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga gttgcaaggt 22500 cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac tccggggcta 22560 tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta tgagatcagg 22620 ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt catcacccag 22680 ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt tttgctgaaa 22740 aagggacacg ggatctatct agacccccag accggtgagg agctgaatac acgcttccct 22800 caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg aggagctgga 22860 agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag aggaggacag 22920 cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag ccgccgccag 22980 accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta ccacctccgc 23040 tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg gtcgcttcca 23100 gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt cctggcgggg 23160 gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat cctttgccag 23220 acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc attactaccg 23280 tcacctccac agcccctact accagcagca agagacagca gaggaaacca gcggcaactc 23340 cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt gcaggcggac 23400 tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg atctttccca 23460 ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa gtaaaaaaca 23520 gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac caacttcagc 23580 gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt 23640 agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc cacacctgtg 23700 cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc cccagatggg 23760 attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca gcgccggtcc 23820 cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac tcctagaaca 23880 gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc ccgccgccct 23940 ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg cccaggccga 24000 agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt gtcgtcaccg 24060 gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc tcaacgacga 24120 gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag ctggttgtgg 24180 gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt cctcgcagcc 24240 ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct cggtctactt 24300 caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga actacgacgc 24360 catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc tgagctagct 24420 cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga tctctgcgag 24480 ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg agttcggatt 24540 accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg gccagtgcta 24600 gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga caaccccgga 24660 ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg aaattagaga 24720 ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact tcagcgggaa 24780 ccagactact cttcactgta aggcttataa ctataagtat cttacttgga tatacaaagg 24840 aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca ccttcctagg 24900 caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc ttcagcccta 24960 ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc acctgattga 25020 aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt ccaactcttc 25080 cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc ttcccccggc 25140 tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg cgggtgggct 25200 cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag tactgtgctg 25260 ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct tttgggtctg 25320 agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga ctgtgtggtt 25380 cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc agaaaaaaac 25440 tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa gtgcggatgg 25500 ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag tttgtttaac 25560 acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag atgactgacc 25620 ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct gagcagcgac 25680 tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc aaggatgcca 25740 ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca aagatttctt 25800 atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag cggcagaaat 25860 tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca gaaattaaag 25920 ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc aagaccctct 25980 gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa taaaacattg 26040 gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc cgtccaagtt 26100 ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc cccggtgggc 26160 tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa caatcttcat 26220 tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg accctgtcta 26280 cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct tcgtttcgtc 26340 agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca acccagtcac 26400 tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag actcagatgg 26460 aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt ccaataacgc 26520 aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat ccttacaagt 26580 tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct atgggcaagg 26640 cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta catttgacag 26700 caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg cgaataaact 26760 gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca ttgaaacaaa 26820 tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa acgttgacag 26880 caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta acgctttccc 26940 tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac ttgtagcttg 27000 ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac ctaattgtac 27060 catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg gcagtcaaat 27120 actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa tcagtgaaaa 27180 aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt taagcaactc 27240 cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag ccgaagccta 27300 tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca caaactctgc 27360 ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta aaccaatgca 27420 tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca taacgttcca 27480 atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct cctttacctt 27540 ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat gttttattta 27600 aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac acagagtaca 27660 cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca gacatattct 27720 taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg atattaataa 27780 actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc acaggctgct 27840 gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac atgggggtag 27900 agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga ataaactgct 27960 gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca gcgatgattc 28020 gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc ctgatctcac 28080 ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc ccacagtgca 28140 aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca tcataccaca 28200 agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac attacctctt 28260 ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta aacatggcgc 28320 catccaccac catcctaaac cagctggcca aaacctgccc gccggctata cactgcaggg 28380 aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg atcatcatgc 28440 tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc ctcaggatta 28500 caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga atcagcgtaa 28560 atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc aaagtgttac 28620 attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc tcaaaaggag 28680 gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt ggtcgtagtg 28740 tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca aaaccttgct 28800 ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc agtgtgatag 28860 ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt tgtcaaaaac 28920 aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc caaacccatc 28980 caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca tggaagaggc 29040 ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat cgcgaaggtg 29100 acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat tgatgcggtt 29160 ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca caaataacag 29220 aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt ccatcaccat 29280 ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct cttgtaaatc 29340 caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc ttaagcacac 29400 cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca aagtccaata 29460 ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa aaaggcttcc 29520 agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg agaagccaaa 29580 ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt acagtatgca 29640 tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg caagcactct 29700 ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg ttgtggaaca 29760 ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta gttctgaaaa 29820 acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa aatgacccta 29880 tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct gtcatcatga 29940 ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct agctgaggaa 30000 tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata gcctctagga 30060 attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa ggtaaaattt 30120 acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc accaggtccc 30180 tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca ggcacagcag 30240 tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata tatagttagt 30300 ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca cacacgccca 30360 aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa aacgtcgtca 30420 cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac cccgcccaaa 30480 acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg ccaatcaccg 30540 cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc catataaggt 30600 atattattga tgatgattta aatcatatgc ggtgtgaaat accgcacaga tgcgtaagga 30660 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 30720 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 30780 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 30840 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 30900 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 30960 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 31020 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 31080 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 31140 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 31200 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 31260 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 31320 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 31380 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 31440 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 31500 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 31560 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 31620 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 31680 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 31740 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 31800 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 31860 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 31920 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 31980 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 32040 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 32100 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 32160 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 32220 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 32280 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 32340 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 32400 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 32460 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 32520 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 32580 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 32640 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttgatttaaa 32700 t 32701 <210> 10 <211> 179 <212> PRT <213> Artificial Sequence <220> <223> BLY6 fiber knob <400> 10 Thr Leu Trp Thr Thr Ala Asp Pro Ser Pro Asn Cys Thr Ile Tyr Thr 1 5 10 15 Asp Lys Asp Ala Lys Leu Thr Leu Cys Leu Thr Lys Cys Gly Ser Gln 20 25 30 Ile Leu Gly Ser Val Ser Val Leu Ala Val Lys Ala Gly Thr Leu Gln 35 40 45 Pro Ile Ser Glu Lys Ile Gly Thr Ala Leu Val Ser Leu Lys Phe Asn 50 55 60 Asn Asn Gly Val Leu Leu Ser Asn Ser Thr Leu Ser Asn Glu Tyr Trp 65 70 75 80 Asn Tyr Arg Lys Gly Asp Val Thr Pro Ala Glu Ala Tyr Thr Asn Ala 85 90 95 Val Gly Phe Met Pro Asn Ile Lys Ala Tyr Pro Lys Asn Thr Asn Ser 100 105 110 Ala Ser Lys Ser His Ile Val Gly Gln Val Tyr Leu Asn Gly Asp Glu 115 120 125 Thr Lys Pro Met His Leu Ile Ile Thr Phe Asn Glu Thr Ser Asp Glu 130 135 140 Thr Cys Thr Tyr Ser Ile Thr Phe Gln Trp Lys Trp Asn Ile Gly Thr 145 150 155 160 Tyr Thr Ser Asp Thr Leu Ala Thr Ser Ser Phe Thr Phe Ser Tyr Ile 165 170 175 Ala Gln Glu <210> 11 <211> 219 <212> PRT <213> Artificial Sequence <220> <223> BLY6 fiber shaft <400> 11 Gly Val Leu Ser Leu Arg Leu Ala Asn Pro Val Thr Thr Lys Asn Gly 1 5 10 15 Glu Leu Thr Leu Lys Leu Gly Asp Gly Val Gly Ile Asp Ser Asp Gly 20 25 30 Asn Leu Thr Ala Gln Thr Val Thr Lys Ala Thr Ser Pro Leu Thr Val 35 40 45 Ser Asn Asn Ala Ile Ala Leu Asn Met Asp Lys Pro Phe Tyr Ser Ser 50 55 60 Asn Gly Lys Leu Ser Leu Gln Val Thr Ser Pro Leu Lys Ile Val Asp 65 70 75 80 Ser Leu Asn Thr Leu Ala Ile Gly Tyr Gly Gln Gly Leu Gly Leu Asn 85 90 95 Asn Ser Ala Leu Ala Val Gln Leu Ala Ser Pro Leu Thr Phe Asp Ser 100 105 110 Asn Ser Lys Ile Lys Ile Asn Leu Gly Ser Gly Pro Leu Lys Ile Asn 115 120 125 Ala Asn Lys Leu Ser Ile Asn Cys Leu Arg Gly Val Tyr Val Thr Thr 130 135 140 Asp Gly Thr Ser Ile Glu Thr Asn Ile Ser Trp Ala Lys Gly Met Arg 145 150 155 160 Phe Glu Gly Asn Ala Met Ala Val Asn Val Asp Ser Thr Lys Gly Leu 165 170 175 Gln Phe Gly Thr Thr Ser Thr Glu Ser Gly Val Thr Asn Ala Phe Pro 180 185 190 Ile Gln Leu Lys Ile Gly Ser Gly Leu Ser Phe Asp Ser Thr Gly Ala 195 200 205 Leu Val Ala Trp Asp Lys Asp Asn Asp Lys Leu 210 215 <210> 12 <211> 44 <212> PRT <213> Artificial Sequence <220> <223> BLY6 fiber tail <400> 12 Met Ser Thr Lys Arg Ala Arg Val Glu Asp Asp Phe Asp Pro Val Tyr 1 5 10 15 Pro Tyr Asp Ala Glu Leu Ala Pro Ser Val Pro Phe Ile Ala Pro Pro 20 25 30 Phe Val Ser Ser Asp Gly Phe Gln Glu Lys Pro Leu 35 40 <210> 13 <211> 30954 <212> DNA <213> Artificial Sequence <220> <223> BLY6.dE1.dE3 Ad vector genome <400> 13 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg atggcctgtg tttgagcata 480 atgtactgac caggtgtaac gttcatctgg gtggtcgtag aggaatgttc atgccatacc 540 aatgcaattt taatcatgtg aggatcttga tggagccgca agcgttttcc agagtcagct 600 tgactggaat ctttgacatg tgtgtggaag catggaagat cttaagatat gatgatacca 660 aatccagatg ccgcgcatgc gagtgcgggg gcaggcatgc caggttccaa cctgtatgtg 720 tggaggtgac cgaggagctg agaccagatc atttggtgct gacctgcact ggtgcggagt 780 tcggttccag tggtgaagaa actgattaaa gtgagtagtg ggatgttata aaagtgacca 840 taaggtgatg tgagatggac aaatttggta atttttatgt atttttgtct tgcagccatg 900 agtgggagcg cttcctttga agggggcgtc tttagccctt atctgacggg gcgtctgcct 960 cattgggctg gagtgcgtca gaatgtgatg gggtctacag tggatggaag acctgttcag 1020 cctgctaatt cttctactct gacttatgct actatgactt cctcgccttt ggatgcagct 1080 gcagctgctg ccgcttctgc tgccgccaac actgttcggg ggatggcctt ggagatgggg 1140 tattatggaa ctgtagtggc caacaccact accccaaata accccacagc cttgaatgag 1200 gacaagctgc tagttctcat gtcccagctg gagtctttga cccaacgcct gggcgatcta 1260 gctcagcagg tgtcccagct gaaggagcag actcaagctg caattaccac tgcgagggga 1320 aattaaaaaa attcaaagaa tcaataaata aaccgagact ttgttgattt taaagtgtgt 1380 cattctttat ttaatttttc gcgcgcgata tgccctggac caccggtctc tatcattgag 1440 gacacggtgg atcttttcta gaacccgata gaggtgggat tggatgttga ggtacatggg 1500 cataagacca tctttggggt gtagatagct ccactgcaga gcctcatgct ccggggtggt 1560 gttgtatata acccagtcat agcatgggcg ttgggcatga tgttgcacaa tatctttaag 1620 gaggagacta atggccactg ggagaccctt ggtgtaagtg tttacaaatc tattaagctg 1680 ggacgggtgc atccgaggtg agataatgtg cattttggat tggattttta gattggcaat 1740 gtttccccct agatctctcc tgggattcat gttatgcaag accactagaa cagtgtatcc 1800 ggtgcactta gggaatttgt catgaagttt ggaggggaaa gcatgaaaaa atttagacac 1860 acccttgtgt cctcccaagt tctccatgca ctcatccata ataatggcaa tgggcccatg 1920 ggcggcggca cgggcgaaca cgttcctggg atctgacaca tcatagttgt ggtcttgggt 1980 caggtcatca taagccattt taataaactt ggggcggagg gtgccagatt gggggatgaa 2040 tgttccctcg ggccccggaa catagtttcc ttcacatatt tgcatttccc aggcttttag 2100 ttcagagggg gggatcatgt ccacctgtgg agcgatgaag aagacggtct cgggggcggg 2160 ggtgattaag tgggaggaca gcaagttcct aagcagctgt gacttgccac acccagtggg 2220 accgtagatg acccctataa caggttgcag atggtagttt agggaaagac agctgccgtc 2280 ctctcgcagg aggggggcga cctcgttcat catttccctc acatgcatgt tttcccgcac 2340 aagttccgat aggaggcgct ctccacccag ggaaaggagt tcttgaagag atgagaaatt 2400 tttcaagggt tttaagccat cagccatggg cattttggag agggtttgtt gcaagagttc 2460 aaggcggtcc cagagttcgg tgatgtgttc tatggcatct cgatccagca tacttcctcg 2520 tttctggggt tgggacggct gcgggagtat ggaaccaggc gatgggcgtc cagcgctgcc 2580 agtgtccggt ccttccacgg tcgcagcgtc cgagtcaggg tcgtttccgt cacggtgaag 2640 gggtgcgcgc ctggctgggc gcttgcgagg gtgcgcttca ggctcatcct gctcgtggag 2700 aaccgctgcc gttctgcgcc ctgtgcatcg gccaggtagc aattaaccat gagttcgtag 2760 ttgagcgcct ctgccgcgtg gcctttggcg cgcagcttac ctttggaagt cttctgacag 2820 gtgggacagt agagacactt gagagcatag agttttgggg ctagaaagac cgattctggg 2880 gagtatgcat cggccccaca ggaggcgcag acggtttcgc attccaccag ccatgtaaga 2940 tcgggctcgt tggggtcaaa aacaagtttt ccgccatgtt ttttgatgcg tttcttacct 3000 ttgctttcca tgagttcgtg cccccgttgg gtgacaaaga ggctgtccgt gtccccgtag 3060 actgacttta tgggcctgtc ctcgagcggc gtgccgcggt cctcttcgta gaggaactcg 3120 gaccactctg agacgaaagc acgtgtccag gccagcacaa aggaggctat atgggagggg 3180 tagcgatcgt tgtcaaccaa ggggtctact ttttccaagg tgtgtaaaca catgtcccct 3240 tcttccacat ccaggaaggt gattggcttg taagtgtatg ccacgtgacc tggggtccca 3300 gacggggggg tataaaaggg ggcgggtctc tgctcgtcct cactgtcttc cggatcgctg 3360 tccaggagcg ccagctgttg aggtaggtat tccctctcga aggcgggcat aacctccgca 3420 ctcaggttgt cagtttctag gaacgaggag gatttgatat tgacagtgcc tgccgagatg 3480 cctttcatga gactgtcgtc catttggtca gaaaagacaa tctttttgtt atcaagtttg 3540 gtggcgaagg atccatacag ggcattggaa agcagtttgg caatggagcg catggtttgg 3600 tttttttctt tgtctgcgcg ctctttggcg gctatgttga gttggacata ttcgcgggcc 3660 agacatttcc attgtggaaa tatggtagtt aattcatctg ggacgattct gactttccag 3720 cctctgttat gcagggtaat cagatccaca ctggttgcca cttctcctct aagtggttca 3780 ttagtccagc atagtcgccc cccttttcga gaacagaaag ggggtagggg atctagcatg 3840 agttcgtctg gggggtctgc atctatggtg aaaatcccag gaaggagatc ttcgtcaaaa 3900 tagctgatgg tggcggggtc atccagagac atttgccatt ctcgagcagc cagagcgcgc 3960 tcgtaggggt taaggggagt cccccatggc atgggatggg tgagtgcaga agcatacatg 4020 ccacagatgt catagacata gagcggctct tccagaatcc ctatgtaagt gggataacat 4080 cgcccccctc tgatgctggc tcgcacataa tcatagagtt catgtgaggg cgctagaaga 4140 cccgagccca ggttggtgcg gttgggtttt tctgctctgt agaggatctg gcgaaagatg 4200 gcatgggagt ttgatgagat ggtgggtctt tggaagatgt tgaaatgggc atgaggcagt 4260 cccacagagt cccttatgaa gtgagcatag gagtcttgca gtttggccac cagctcggcg 4320 gtgaccagca catccaaagc acagtagtcg agggtctctt tgatgatgtc atagttaggt 4380 tcccctttct tttcccacag ctcgcggttg agaaggtatt cttcgcgatc cttccagtac 4440 tcttcgaggg ggaacccgtc cttgtctgaa cggtaagaac ccagcatgta aaattgattg 4500 acagctttgt aggcacaaca ccccttctcc acggggagtg agtatgcttg cgcggctttg 4560 cgcagagagg tgtgagtaag ggcgaaagtg tccctgacca tgactttgag gaactgatgc 4620 ttaaagtcta tgtcatcgca ggccccctgc tcccacagtt ggaagtccac tcgctttttg 4680 taggcgggat tgggcaaagc gaaagtaaca tcgttgaata ggatctttcc agccctgggc 4740 atgaagttgc gagtaatgcg aaaaggctga ggcacttctg ccctgttgtt gataacttgg 4800 gcagccaaga cgatctcgtc aaagccgttg atgttgtgac ccacaatgta aagttctacg 4860 aagcgtgggc gtcccttgat gtggggcagt tttttaagct cttcgtaggt caagtcgtca 4920 gggtcagcga ttccatattg ctccaaagcc cagtcaggca ggtgaggatt agcatgaagg 4980 aaagaggtcc aaagatccac ggccagagct gtttgtaagc ggtctctgta ctgacggaaa 5040 tgtcggccta ccgccatttt ttcaggagta acacagtaaa aggtgcgcgg gtccttttcc 5100 cagcgatccc attgaagttg caaggctagg tcgtgggcga ggttgacgag ctgttcgtcc 5160 cccgaaagtt tcatgaccag catgaaaggg acaagctgct tgccaaagga ccccatccag 5220 gtgtaggttt ccacatcgta ggtgaggaag agcctttctg tgcgaggatg agaaccgatc 5280 gggaagaact ggatttcctg ccaccagttg gaggaatggc tgttgatatg atggaagtag 5340 aactccctac ggcgcgccga gcattcgtgc ttgtgcttgt acagacggcc acagtactcg 5400 cagcgctgca cgggatgcac ctgatgaatg agctgtacct ggcttccttt gacaagaaat 5460 ttcagtggga agttgaggcg tggcgtctgc atctcgtgtt gtattacgtc ctggctattg 5520 gtctggccat cttctgtctc gatggtggtc atgctgacga gcccgcgcgg gaggcaggtc 5580 cagacctccg cgcggacggg tctgagagcg aggacgagag cgcgcaggcc ggaactgtcc 5640 agggtcctga gacgctgcgg agtcaggtca gtagggagag tacataggtt tacttgcata 5700 agtttttcca gggcatgtgg gaggtcaaga tgatatttga tttctactgg cgagttggtg 5760 gagacatcga tggcttgcag ggtcccgtgc ccctggggtg ctaccaccgt cccttttttt 5820 ttcttgatcg ggggcggtgt tgcttcttgc atggtaaggt cgtcttctag aagcggcggc 5880 gaggtcgcgc gccgggtggc agtggcggtt ctggacctgg aggtagaggc ggtagaggta 5940 cgtcggcgcc gcgcgcgggt aggttctggt actgcgccct gagaagactt gcgtgagcga 6000 cgacgcggcg gttgacgtcc tggatctgac gcctctgggt gaatgctacc ggacccgtga 6060 gcttgaacct gaaagagagt tcaacagaat caatttcggt atcgttgacg gctgcctgcc 6120 gcaggatttc ttgtacgtcg cccgagttgt cttggtaggc gatctcggcc atgaactgct 6180 cgatctcttc ttcttggaga tctccgcggc ccgctcgttc tacggtggca gcaaggtcgt 6240 tggagatgcg ccccatgagc tgtgagaatg cattcatgcc cgcctcgttc cagacgcgac 6300 tgtagaccac ggctccctcg ggatctctgg cgcgcatgac cacttgggcg aggtttagtt 6360 ccacgtgtct ggtgaagacc gcatagttgc agagacgctg gaagaggtag ttgagcgtgg 6420 tggcgatgtg ctcggtgaca aagaaataca tgatccagcg acgaagcggc atctcgctga 6480 tatcgcccag ggcttccaac cgttccatgg cttcgtaaaa gtccacggcg aagttgaaaa 6540 actgggagtt gcgagcggac acggtcaact cctcctccag aagacggatg agctcggcga 6600 tggtggcgcg cacttcgcgc tcaaaggctc ccgggatctc ttcctcctct tcttcttcca 6660 actcttcctc cactaacatc tcttctactt cctcctcagg cggcgggggt ggaggagggg 6720 gcgcgcggcg acgccggcga cgcacgggca gacgatcgat gaagcgttcg atcacttctc 6780 cgcggcggcg acgcatggtc tcggtgacgg cgcgcccgtc ctccctgggt cgcagagtga 6840 agacgccgcc gcgcagctcc ctgaaatggt gactgggagg gtccccgttt ggtagggaca 6900 gggcactgat gatgcatctt attaattgcc ctgtagggac tccgcgcaag gacctgagcg 6960 tctcgagatc cacgggatct gaaaatctct gaacgaaggc ttcgagccag tcgcagtcgc 7020 aaggtaggct gagcactgtt tcttcggggc gggctgctga gctagagggt tgtacgatgc 7080 tgctggtgat gaagttaaaa taggcagttc tgagacggcg gatggtggcg aggagcacca 7140 ggtctttggg tccggcttgc tggatgcgca ggcggtcggc cattccccat gcattatctt 7200 ggcacctggc cagatcttta tagtagtctt gcatgagtcg ctccacgggc acttcttctt 7260 cgcccgctct gccgtgcatg cgtgtgagtc cgtaccctct ctgtggttgg acgagcgcca 7320 ggtcggcaac gaccctttcg gctagaatgg cttgctgcac ctgggtgagg gtgttctgga 7380 aatcatcaaa gtccacaaag cggtggtagg cccccgtgtt gatggtgtag gagcagttgg 7440 acatgaccga ccagttgact gtctggtgtc ctggtcgtac gagttccgtg tacctgagcc 7500 gcgagtatgc gcgggagtcg aagatgtaat cgttgcaggt tcgcaccagg tactggtagc 7560 cgatgaggaa gtgaggcggc ggctggcggt agagaggcca tcgttcggtg gcgggcgcgc 7620 cgggcgctag gtcttctagc atgagacggt ggtatccgta gacgtacctg gacatccagg 7680 taataccggc ggcggtggtg gaggcgcgcg gaaactctcg cacgcggttc cagatgttgc 7740 gcagcggcat gaagtagttc atggtgggca cggtctggcc cgtgaggcgc gcgcagtcat 7800 tgatgctcta gatacgggca aaaacgaaag cgttgagcgg ttcccttccg tggcctggag 7860 gaacgcgaac gggttaggtc gcagcgtacc ctggttcgag actaaagaaa gcgagcaact 7920 cgaaccggca gagtcgcggc taacgggtat tggcaatccc gtctcgaccc aagccagcaa 7980 atccaggata cggatggggg ccccttttgt ttttcagggc atgagtcacc ggttaaggtt 8040 tacaacggct gtttcatgcc tttagaagtg gctcgcgccc gtagtctgga gaatcaatcg 8100 ccagggttgc gttgcggcgt gccccggttc gagcctgcag cttgagtcgg ccggtgaccg 8160 cggcaaacga gggcgtggcg gccccgtcgt ttctaagacc ttgctagccg acctctccag 8220 tttacgggaa cgagccccct tttatttttt ttgtttttgc cagatgcatc ccgtactgcg 8280 gcagatgcgc ccacagcccc cacagcagca gcagcaggct ggcctacctt ctctacctca 8340 gccgctacct gcaactaccg cggtggccgc tgtaagcggg gccggacagc aggcggctcc 8400 tcaatatgaa ttggacttgg aagagggcga gggattggca agattggggg cgccctcgcc 8460 cgagcgccac ccgcgggtgc agatgaaaaa ggacgttcgc gaatcttacg tgcccaagca 8520 gaatctgttc agagacagaa gcggcgagga gcccgaggag atgcgcgcgt cccgttttaa 8580 cgcgggtcgc gagctgcgac aaggactgga tcgaaaacgg gtgttgaggg atgatgattt 8640 tgaggtggat gaaatgacag ggatcagccc cgctcgcgct cacgtggctg cagctaatct 8700 ggtgacagct tatgagcaga ccgtgaagga ggaaagcaac ttccagaaat cattcaataa 8760 ccacgtgcgc accctgatcg cacgcgagga ggtgaccctg ggcctgatgc acctgtggga 8820 tctgctggaa gccatagtgc agaaccccac tagcaaaccc ctgactgctc aactgtttct 8880 ggtggtgcag cacagcaggg ataatgaggc attcagagag gcgctgctga atatcactga 8940 acctgagggg agatggctgc tggatctggt gaatatcctg cagagcattg tagtgcagga 9000 acgcagcttg cctttgtccg agaaggtggc ggcgatcaat tactctgtgc tgagtctggg 9060 caaatactat gccaggaaga tctacaaaac cccttacgtg cccatagaca aggaagtgaa 9120 aatagatggg ttttacatgc gcatgaccct gaaagtgcta accctgagcg atgacttggg 9180 agtgtaccgc aacgacagga tgcaccgcgc ggtgagcgcc agcaggaggc gcgagctgag 9240 cgacaaagaa ttaatgcaca gcttgcaacg agccctgacg ggagccggga cggaggggga 9300 gaactacttt gacatgggtg cagacttgca ttggctgcct agtcgcaggg cattggaagc 9360 ggcaggcgat gggccctatg tagaggaagt agtagacgag gacgatgagg agggcgagta 9420 cctggaagac tgatggcgcg acccgtattt ttgctagatg gaacaggcgc cggaccctgc 9480 gatgcgggcg gcgctgcaga gccagccgtc cggcattaat tcctcggacg attggaccca 9540 ggccatgcaa cgcatcatgg cgctgacgac ccgcaacccc gaagccttta gacagcagcc 9600 tcaggccaac cgcctttcgg ccatcctgga ggccgtggtg ccctctcgct ccaaccccac 9660 ccacgagaag gttctggcca tcgtgaatgc cctggtggag aacaaggcca tccgctccga 9720 tgaagccggg ctggtataca acgccttgct cgagcgcgtg gctcgctaca acagcagcaa 9780 tgtccagact aacctggaca ggatggtgac cgacgtgcgc gaggccgtgt cccagcgcga 9840 acggttccat cgcgagtcta acctgggttc catggtagcg ctgaacgctt tcctcagttc 9900 ccagcctgcc aatgtgcccc ggggacagga agactatacc aactttatta gcgccctgag 9960 actcatggta gccgaggttc ctcagagcga ggtgtaccag tccggtccag actacttttt 10020 ccagacaagc aggaacggta tgcagacagt gaacttaagc caggctttca agaacctgca 10080 agggctgtgg ggagtccaag ctccagtggg cgacagggcg accgtgtcga gcctgttgac 10140 tccaaattcc cgtttgctgc tgctgctggt gtcccccttc actgacagcg gcagcataaa 10200 cagaaactcc tacttgggct acctgataaa cttgtatcgc gaagctatag gtcaggccca 10260 cgtggacgaa cagacctatc aggagatcac taatgtgagt cgcgctctgg gccaggacga 10320 ccctggaaac ctggaagcta ctctaaactt tctgctgacc aaccgctcgc aaaaaatccc 10380 tcctcagtat acattaactg cggaggagga acggatcttg agatacgtgc agcagagcgt 10440 gggtctgttc ctgatgcaag agggtgcgac ccctagcgcc gcgcttgata tgacagcgcg 10500 caacatggag cccagcatgt atgccagcaa cagaccattc attaataaat tgatggatta 10560 cttccatcgc gcggccgcta tgaactctga ttacttcacc aatgctattc tgaaccccca 10620 ttggctgcct ccgcctggtt tttatactgg cgagtatgac atgcctgacc ccaacgatgg 10680 gttcttgtgg gacgatgtgg acagcgtggc gttctcgcct accgctcctc gtactttttg 10740 gaagaaggaa ggtagtgaca gaagaccctc ctccgtgctg tcaggacgtg agggtgctgc 10800 cgcggcggtc cccgatgctg caagcccctt tcccagtctg ccattttcac taaacagcgt 10860 gcgcagtagc gagctgggga gaataacccg ccctcgcttg ctgggcgagg acgagtattt 10920 gaatgactcc ctactgagac ccgagcggga aaagaacttc cctaataatg ggattgaaag 10980 cctggtggat aagatgagca gatggaagac ctatgcccag gagcacagag atgagcctag 11040 aatcttgggt cctacagtag gcacccgcag acgccagcgc catgatagac agcggggtct 11100 ggtgtgggac gatgaggatt ctgcagatga cagcagcgtg ttggacttgg gcgggagggg 11160 aggtgtgggc aacccgttcg cacacttgcg tccccgtatt ggacgcatga tgtaaaagtg 11220 aaaataaaaa aggaactcac caaggccatg gcgaccagcg tgcgttcgtt ctttctgttg 11280 ttgtatctag tatgatgagg cgcaccgtgc taggcggatc ggtggcgtat ccggagggtc 11340 ctcctccttc gtacgaaagc gtgatgcagc aggtggcggc ggcggcgatg caacccccct 11400 tggaggctcc ttacgtgccc ccgcggtacc tggcacctac cgaggggaga aacagcattc 11460 gttattcgga actcacaccc ttgtatgaca ccacccggtt gtacctggtg gacaacaaat 11520 cggcggacat tgcctcgttg aactatcaga acgaccacag caacttcttg acaacggtgg 11580 tgcagaacaa tgactttacc cccacggagg ccagcaccca gaccatcaac tttgacgagc 11640 gctcccggtg gggcggtcag ctgaagacca tcatgcacac caacatgccc aacgtgaacg 11700 agttcatgtt tagcaacaag ttcagggcta gggtgatggt gtccagaacc acacctaaag 11760 aggtgacagt cacaacagac tatgatggta gtcaggacat cttggaatac gagtgggttg 11820 actttgagtt accagaaggc aacttctctg ccaccatgac catagacctg atgaataatg 11880 caattgttga taattaccta aaagtgggta gacagaatgg ggtactggag agtgacatag 11940 gtgttaagtt tgacactagg aactttaggc ttggttggga cccagtgaca gagttggtca 12000 tgcctggggt ctacaccaat gaagctttcc atcctgacat agtcctacta cctggctgcg 12060 gagtggactt cactgagagc cgcctcagta atctgctagg cattagaaag aaacagccat 12120 tccaggaagg gttccagatc atgtatgagg atctggaggg tggtaacatc cccgccctgc 12180 ttgatgtaaa tgcatatgag aagagcaagg aagataatac aaccaccaca aatgaagctg 12240 tggccgcggc ttcatctact gaagccaaag ctgtggtaga tgcttccact tcaacagaaa 12300 acaccactga tgaaaaagtc accaggggag atacatttgc cacccctgaa caagagaagg 12360 cagctgaggc agagtctgat attatgcttc tgtccaccga tgaaaacgaa actaaaaaac 12420 aactggttat tcgagcggtg accaaggata gtaaggacag gagttataat gtattgtcag 12480 atggaaagaa cacagcttac cgtagttggt acctggcata caattatggc gaccgtgaga 12540 aaggggtgcg ttcttggaca ctgcttacca cctcggatgt cacctgcggc gtggagcaag 12600 tctattggtc gctaccagat atgatgcaag atccagtcac ctttcgctcc acacgccaag 12660 ttagcaacta cccagtggtg ggcgcagagc tgctcccagt gcattccaga agcttctaca 12720 acgagcaagc cgtctactcg caacagctcc gccagtacac ctcgctcacg cacgtcttca 12780 accgcttccc cgagaatcag atcctcgtcc gcccgcccgc gccaaccatt accaccgtca 12840 gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gctgcgcagc agtatccggg 12900 gagtccagcg cgtgaccgtt actgacgcca gacgccgcac ctgtccatac gtatacaagg 12960 ccctgggcat agtcgcgccg cgcgtccttt caagccgcac tttctaaaaa aatgtccatt 13020 ctcatctcgc ccagtaataa caccggttgg ggcctgcgca cacctagcaa gatgtatgga 13080 ggcgctcgca gacgctccac tcagcaccct gtgcgcgtgc gcgggcattt ccgcgctccc 13140 tggggcgccc tcaagggacg ctctcgtact aggaccaccg ttgacgatgt gatcgaccag 13200 gtggtcgccg atgcacgtaa ctataccccc gcagccgcac ctgcatccac cgtggatgcg 13260 gtcattgaca gcgtggtagc cgatgcgcgc gcctatgctc gcgccaagag caggaggcgg 13320 cgtattgcca ggcgtcaccg agctactcca gccatgcgag ctgcaagagc tttattgcgg 13380 agagccagac gtgtggggcg aagagccatg cgtagagcgg ccagacgcgc ggcttcaggt 13440 gccagcgcag gcagggtccg caggcgcgcg gctacggcgg cagcggcggc catcgctagc 13500 atgaccaaac cacgaagagg caatgtgtat tgggtgcgcg acgccgccac cggccagcgc 13560 gtgcccgtgc gcacacgccc ccctcgcact tagaagatac tgagcagtct ccgatgttgt 13620 gtcccagcgg cgagatgtcc aagcgcaaat tcaaggaaga gatgctccag gtcatcgcgc 13680 ctgagatcta cggtcctgcg gtgaaggatg aaaaaaagcc ccgcaagatc aagcgggtca 13740 aaaaggacaa aaaggaagaa gatggtgatg atgggctggt ggagtttgtg cgcgagtttg 13800 ccccaaggag gcgcgtgcag tggcgcgggc gcaaagtgtg gccggtgttg agaccgggga 13860 ccacagtggt ctttacgcca ggcgagcgct ccagcaccgt ttccaaacgc tcttatgatg 13920 aggtgtacgg ggacgatgat attctcgagc aggcggctga tcgccttggc gagtttgcat 13980 atggcaaacg cagccgctcg ggagccaagg aagaggcatt gaccatcccc ttggatcatg 14040 gaaatcccac cccaagcctc aaacccgtga ccctgcaaca agtgctgccc acgccgccac 14100 gcaagggcat caagcgcgag ggcgaggatc tgtatcccac catgcagctg atggtgccca 14160 agcgccagaa gctggaagac gtgctggaga aaatgaaagt ggatcctgaa atccagcctg 14220 aagtcaaagt gaggccaatc aagcaggtgg cgcccggttt gggggtacaa accgtggata 14280 tcaagatccc caccgagtcc atggaaattc aaaccgaacc catgaagccc acctccagca 14340 ccattgaggt gcagacggat ccttggatgc ccgcgcctgc tcctgttacc actactactc 14400 gaagacctag aagaaagtat ggttcagcca acctgataat gccaaactat gctctgcatc 14460 catcaatcat acccactcct ggctaccgcg gcactcgcta ctaccgcagt cacagcaccc 14520 gccgacgtaa agcacctgcc acccgccgcc gtcgccgccg ccgtgccact agcaaactta 14580 ccccctcggc tatggtgcgg agagtgtacc gtgatgggcg cgcagctcct ctgacactgc 14640 cgcgcgcgcg ctaccatcct agcattgcca tttaacaact ctgcctcctt gcagatatgg 14700 ccctcacttg ccgccttcgt attcctattg ctggctaccg cggaagaaag tcgcgccgta 14760 gaagagcagg gttgtctggg agcgggatgc gtcgccaccg gcggcggcgc gccatcagca 14820 aacggttggg gggtggattt cttcccgctt tgattcccat catcgccgcg gcgatcggcg 14880 cgataccagg catagcttcc gtggcggtgc aggcctcgca gcgccactga cattggaaaa 14940 agatatctta taaataaaaa tagaatggac tctgacgctc ctggtcctgt gatatgtttt 15000 tgtagacgag atggaagaca tcaatttttc atccctggct ccgcgacacg gcacgcggcc 15060 gtatatgggc acctggagcg acatcggcaa cagccaactg aacgggggag ccttcaattg 15120 gagcagtcta tggagcgggc ttaaaaattt tgggtccact ataaagactt atgggaacaa 15180 agcttggaac agcagcacag ggcatgcgct gagacaaaag cttaaagatc agaatttcca 15240 acagaaggtg gtcgatggta tcgcctctgg aatcaatggg gtggtagatc tggccaacca 15300 ggccgtgcag aaacagatta acagtcgcct ggacccggct cccccagctc ctattcatga 15360 gttaatgcaa gtggaggaag agctcccttc attggaaaag cggggcgata agcgacctcg 15420 tccagatatg gaggaaacgc tgctgaccaa ggtggatgag ccgccctcct atgaagaggc 15480 tgtaaaactg ggaatgccca ctacaaagcc cattatgcct ctggccactg gagtgatgaa 15540 gccatctcag tctaaacctg cagttgctgc tacattggac ttgcccgctc ccgtggccac 15600 ccccaaacct gtcgccgccc cgaagcccac cgccgtgcaa cccgtggccg tggccagacc 15660 gcgtcccggt ggtcggccga atgcaaactg gcagagcact ctgaacagca tcgtgggttt 15720 gggagtgcac agtgtgaagc gccgtcgctg ctattgatta aatatggagt agcgcttaac 15780 ttgcttgtct gtgtgtgtat atgtcgatgc cgcccgccgt gctacagcaa agagagaagg 15840 agaagaggcg ccgctgagtt cctttcaaga tggccacccc atcgatgctg ccccagtggg 15900 cgtacatgca catcgccgga caggacgctt cggagtacct gagtccgggt ctggtgcagt 15960 tcgcccgcgc cacagatacc tacttcaatc tggggaacaa gtttaggaac cctaccgtgg 16020 ctcccaccca cgatgtgacc accgaccgta gccagcgcct gacgctgcgc tttgtgcccg 16080 ttgaccggga ggacaatacc tactcctaca aagtcagata caccctggct gtgggagaca 16140 acagggtgtt ggatatggcc agcacctact ttgacatcag gggcgtgttg gacagaggac 16200 ctagcttcaa accatactct ggcactgcct acaactccct ggctccaaaa ggagctccaa 16260 actccagtca gtggcaacaa aaggaaaaca atggtcaagg tgatgcaaag actcacacct 16320 atggtgtagc tgccactgga ggtattgaca ttgacaaaaa tggtcttcaa attggaatcg 16380 atgaaactaa agaagataat aacgaaattt atgcagacaa aacattccaa cctgaacctc 16440 aaattggaga agaaaactgg caagatagcg aaaactatta tggaggcagg gctcttaaac 16500 cggaaaccaa gatgaagcct tgctatggtt ccttcgctag accaactaat gcaaagggag 16560 gtcaagccaa aattaaacca gctcaagagg gtcaacagtc tatagattat gacatagacc 16620 tggctttctt tgatattcca agcactggcg gaggcaatgg cacaaatgta aatgacaagc 16680 cagatatggt tatgtatact gaaaatgtaa atctggaaac tccagacact catcttgttt 16740 acaagccagg aacttcagat gacagttccg aggccaattt aactcagcaa gccatggcta 16800 acagacccaa ctatattggg tttagagata actttattgg cgtcatgtac tacaacagca 16860 ctggcaacat gggagtgctt gctggtcaag catcccagct aaatgctgtg gtggacctgc 16920 aagacagaaa caccgagctg tcttatcagc tattacttga ctctctgggc gacagaacca 16980 ggtattttag tatgtggaat caggcggtgg acagctatga tcctgatgtg cgcattattg 17040 aaaaccatgg tgtggaagat gaattgccaa actattgctt cccattggac ggagctggca 17100 ctaatgctgt ttaccaagga gttaagacaa aagaggataa taatggcgaa tgggaaacag 17160 acacaaatgt tgcatcgcag aatcagatat gcaagggcaa catatatgct atggagatca 17220 acctgcaagc caacctgtgg aaaagtttcc tttactccaa cgtggctctg tacctaccag 17280 actcctacaa gtacactcca tccaacgtga cactccctac caacactaac acctatgact 17340 acatgaatgg cagggtggtg tctccatccc tggtggatgc ctacattaac attggcgcca 17400 ggtggtctct ggatgccatg gacaatgtca accctttcaa ccaccaccgc aatgccggcc 17460 tgcgctaccg gtccatgctt ctgggcaacg gccgatacgt gcccttccac atccaagtgc 17520 cccagaaatt cttcgctatc aagaacctgc tgcttctccc aggctcatac acctacgagt 17580 ggaacttccg caaggatgtc aacatgatcc tgcagagttc ccttggcaat gacctcagaa 17640 ccgatggggc caccatccag tacaccagca tcaatctcta tgccaccttc ttccccatgg 17700 ctcacaacac tgcctccacc ctggaagcca tgctgcgcaa tgacaccaat gaccagtcct 17760 tcaatgacta cctctcagct gccaacatgc tttaccccat ccctgccaat gccaccaacg 17820 tgcccatctc catcccatct cgtaactggg ctgccttcag gggctggtct ttcacccgcc 17880 tcaagaccaa ggagacccca tctctgggat cagggttcga tccctacttc gtctactcag 17940 gctccattcc atacctggat ggaactttct accttaacca cactttcaag aaagtctcca 18000 tcatgtttga ctcttctgtc agctggccag gcaatgacag gctgctgact cccaatgagt 18060 tcgaaatcaa gcgcactgtt gatggggaag ggtacaatgt ggcacaatgc aacatgacca 18120 aagactggtt cctggttcag atgctctccc actacaacat tggctaccag ggcttctaca 18180 tcccagaagg atacaaggac cgcatgtact ccttcttcag aaacttccag cccatgagcc 18240 gccaggtggt cgatcaggtc aactacaaag actacatggc agtcaccctt gcctatcagc 18300 acaacaactc tggctttgtg ggctacctcg cgcccaccat gcgacagggc caaccctacc 18360 ctgctaacta cccatacccg ctcattggca agactgcagt caacagtgtc acccagaaaa 18420 agttcctctg cgacagggtc atgtggcgca tccccttctc cagcaacttc atgtccatgg 18480 gggcccttac cgacctgggg caaaacatgc tttatgccaa ctccgcccac gcgctagaca 18540 tgaatttcga agtagacccc atggatgagt ccacccttct ctatgttgtc ttcgaagtct 18600 tcgacgtggt cagagtgcac cagccccacc gcggcgtcat cgaagctgtc tacctgcgca 18660 cccccttctc agctggtaac gccaccacat aagcgccttg cttcttgcaa gtggctgcag 18720 cagcatggcc tgtggatcct ccactggatc caatgagcaa gagctcaggg ccatcgccat 18780 agacctgggc tgtggaccct atttcctggg aacctttgac aagcggtttc caggcttcat 18840 ggctcctgac aagctcgcct gtgccattgt caacacggca gggcgcgaga ctggtggtga 18900 gcactggctg gcttttggat ggaacccccg ctccaatacc tgctatctct ttgacccgtt 18960 tgggttttca gacgagcgcc tcaagcagat ctatcaattc gagtacgagg ggctcctgcg 19020 ccgcagtgcc ctggctacta aggaccgatg catcactctg gaaaagtcta cccagaccgt 19080 gcagggtccg cgctcggctg cctgcgggct cttctgctgc atgttcctcc atgcttttgt 19140 gcactggccc gaccgcccca tggacaacaa ccccaccatg aatttgctga cgggggtacc 19200 caacaacatg ctccaatcgc cccaagtaga gcccaccctg cgccacaacc aggaggcact 19260 ctatcgcttc ctgaactccc actcatctta ctttcgttct aaccgcgcgc gcattgagaa 19320 ggccactgcc ttcgatcgaa tgaataataa catgtaaacc aaattgtgtg tggctcaaat 19380 aaacagcact ttattgttta catgcactga ggctctggga tgatcatttt ttaaaaatcg 19440 aaggggttct ggcgggaatc agcatggcca gatggcaggg acacgttgcg gaactggaac 19500 ttgttctgcc acttgaactc gggaatcacc agcctgggaa ctggaatctc tggaaaggta 19560 tcttgccata gctttctggt cagttgcaga gcgccaagca ggtcaggagc agatatcttg 19620 aaatcacagt tggggccaga attctgggcg cgggagttgc ggtacactgg gttgcagcac 19680 tggaacacca taagggcagg gtgtctcacg ctcgccagca cggtctcgtc actgatgcaa 19740 gacacatcca ggtcttcagc attggccatt ccaaaggggg tcatcttgca ggtctgtctg 19800 cccatcacgg gagcgcagcc aggtttgtgg ttgcaatcac aatgaagggg gatcagcatc 19860 atcttggcct ggtcgggggt aatccctggg taaacagcct tcatgaaggc ttcatactgc 19920 ttgaaagctt cctgggcttt ggttccctcg gtgtagaaca ctccacaaga cttgctggaa 19980 aactgattag tagcgcagtt ggcatcattc acacagcagc gggcgtcgtt attagccagc 20040 tggaccacat tcctgcccca gcggttctgg gtgatcttgg ctcgatctgg gttctccttc 20100 aacgcgcgct ggccgttctc gctcgccaca tccatctcaa tgacatgttc cttctggatc 20160 atgatgttgc catgcaggca tctaatcttg ccttcataat cagtgcagcc atgaggccac 20220 agcgcgcacc cggtgcactc ccaattgtta tgggggatct gggaatggct atgaaccagc 20280 ccttgcagga atcttcccat catcacagcc agggtcttta tgctggtaaa ggtcagcggg 20340 ataccgcggt gctcctcgtt cacatactgc tggcagatgc gtctgtagtg ctcggcctgc 20400 tcgggcatca gcttgaaaga ggttttcaac tcattatcca gcctgtatct ctccatcatg 20460 atggacatta cttccatgcc cttctcccag gcagaaacaa tagggagact caggggattc 20520 ttgacagtag agacaacctt acttaagggg tcatcactgc caatcttttc gatgcttctc 20580 ttgccatcct tctcggtgat gcgcaccggc gggtagctga atcccacagc caccaactga 20640 gcctcttccc tttcgtcttc gctgtcttga ctgatgtctt gcagaggaac atgtttggtt 20700 ttcctgggtt tcttcttggg cggcagctct ggaggactct ggctccgttc cggagacccc 20760 atggatgagc gagagttgtc gctcaccact tggatctggc tgcctgtaga agaactggac 20820 cccacgcggc ggtaggtgtt cctcttggta ggcagaggtg gaggcgacgg gctccggtcc 20880 ggtctgggtg gcggatggct ggcggagccc cttccgcgtt cgggggtgcg ctccagatgg 20940 cggtcgtctg actgacctcc gcggctggcc attgtgttct cctaggtaga gaaacaagac 21000 atggagactc agccatcgct gccatcgcca tccaccacca caagcaccgc cgaggaggag 21060 gagtgtttaa ccaccccacc atgcagcccc gctaccacca ccagcaccct tgaaagcgag 21120 gtcgacacgg tcgtggagga tttacaggct atggaagata ttgaggcagc tgtcgagcaa 21180 gaccccggct atgtgacacc ggcggagcat gatgaggatc tagcgcgctt tctcgacggt 21240 gtggagaaag cgaaacaaga tgaggacgag gaagaggcag aagcacaacc atcggtggcc 21300 gactacctca ccggcctagg gctagaagac gtgctgctta agcatcttgc aaggcagaca 21360 gtcatagtca aagacgccct gctagagcgc tccgaggtgc cactcagtgt ggaagacctc 21420 agtcgcgcct atgagctaaa cctcttctcg cctcgcaagc cccccaagcg tcagcccaac 21480 gggacctgtg agcccaatcc gcgcctcaac ttctatccag ccttcactgt gcccgaagta 21540 ctagctacct accacatctt tttcaagaac caaaagatcc ccatctcctg ccgcgccaat 21600 cgcacccgcg cagatgccct actcaacttg gggcccggcg ctcgcatacc tgatatcgct 21660 tccttggaag aggttcctaa gatctttgag ggtctgggca atgaggaaac tcgggcagca 21720 aacgctctgc aaagagaaac agatgatggt gaacaccaca gcgctctggt ggagctccag 21780 ggcgacaacg ctcgtcttgc agtcctcaaa cgcagcatcg aggtcaccca tttcgcctac 21840 cccgcactta atctcccacc caaagtcatg agctcggtca tggacacgtt gctcatgaag 21900 cgcgcgagcc ccatctccga ggatcagaac atgcaggacc ccgatgcctc agatgaaggc 21960 aagcctgtag tcagcgacga gcaactggct cgctggctag gctctgactc cccccagtct 22020 ttggaggagc ggcgcaagct tatgatggca gtggtcctga tcacagcgga gctggagtgt 22080 ctccgccgct tcttcactga cccagagacc ctgcgcaagc ttgaggagaa cctgcattac 22140 acattcagtc atgggttcgt gcgccaggcg tgcaagatct ccaacgttca actcaccaac 22200 ctggtctcct acctgggcat cttgcatgaa aaccggctgg ggcagaacgt gctccacacc 22260 accctgaagg gggaggcccg ccgcgactat atccgcgact gtatctacct ctacctatgc 22320 tacacctggc aaagcgggat gggtgtgtgg caacagtgct tggaagagca aaatctaaaa 22380 gagctggaaa agctgcttca gaaatctctt aaatctctgt ggaccgggtt cgatgagcgg 22440 accaccgctt cggacatggc cgatattatc ttccccgagc ggctcagaca cactctgcgc 22500 gacgggctgc ctgactttgc cagccagagc atgctacaaa actttaggtc attcatcttg 22560 gaacgctccg ggatcctgcc cgccacttgc tgcgcactgc cctccgattt tgtgcccatc 22620 acctaccggg agtgcccccc gccgctatgg agccactgct acctgttccg cctggccaac 22680 tacttggcct accactctga tgtgatagaa gatgttagtg gcgaagggct cctggagtgc 22740 cactgccgct gcaacctctg caccccccac cgctccctcg cctgcaatcc ccagctgctg 22800 agcgaaaccc agatcatcgg caccttcgag ttgcaaggtc ccagcggcga aggcgagggg 22860 tcctctccgg ggcaaagtct gaaactgact ccggggctat ggacctccgc ttaccttcgc 22920 aagttcgccc ccaaagacta ccacccctat gagatcaggt tttatgaaga ccaatcacag 22980 ccccccaagg ccgaactgac ggcctgcgtc atcacccagg gggcaatctt ggcccaattg 23040 caagccatcc aaaaatcccg ccaagaattt ttgctgaaaa agggacacgg gatctatcta 23100 gacccccaga ccggtgagga gctgaataca cgcttccctc aggatgcccc gaggaggcaa 23160 gagaatgaaa gttcagatgc cgcccgagga ggagctggaa gactgggaca gtcaggcaga 23220 ggaggaagac tgggacagcc aggcagaaga ggaggacagc ctggaggagg acagtctgga 23280 ggaaggcgag gagcccaagg aagaggcagc cgccgccaga ccatcgtcct cggcggtgga 23340 gacaagcaag gtcccagaca gcacggctac cacctccgct ccagctcaag gggccgctcg 23400 gcgacccaac agtagatggg acgagacggg tcgcttccag aaccccacca ccgtcaagac 23460 cggtaagcag gagcggcagg gatacaagtc ctggcggggg cataaaagtg ccatcatcgc 23520 ttgcttgcag gagtgtgggg gcaatatatc ctttgccaga cgctacctgc tattccatca 23580 cggggtgaat ttcccccgca acatcttgca ttactaccgt cacctccaca gcccctacta 23640 ccagcagcaa gagacagcag aggaaaccag cggcaactcc gagagttaga aaaccagcag 23700 ctaaaaaatc cacagcggcg gcagcaggtg caggcggact gaggatcacc gcgaacgagc 23760 cagctcagac cagggagttg aggaatcgga tctttcccac cctctatgcc atattccaac 23820 aaagtcgggg tcaggaacaa gaactgaaag taaaaaacag atctcttcgc tcgctcaccc 23880 gcagttgttt gtatcacaag agcgaagacc aacttcagcg cactctcgag gacgccgagg 23940 ctctcttcaa caagtactgc gcgctgactc ttaaagagta gactgcgcgc gcttggcgag 24000 aaaaggcggg aattacgtca cctcttggcc acacctgtgc ttcattatga gtaaagaaat 24060 tcccacgcct tacatgtgga gctatcagcc ccagatggga ttggccgctg gcgccgccca 24120 ggactactcc acccgcatga attggctcag cgccggtccc gcgatgatct cacgggttaa 24180 tggtgtgaga gagcaccgaa accagatact cctagaacag tccgccctca ccgccactcc 24240 ccgcaatcac ctcaaccccc gtaattggcc cgccgccctg gtgtaccagg aaactcctgc 24300 tcccactaca gtactacttc ctcgtgacgc ccaggccgaa gttcagatga ctaactcagg 24360 tgtacagctg gcgggtggtg ccaccctgtg tcgtcaccgg ccaagaccgg gtataaaggg 24420 cctggtgatc agaggccgag gtattcagct caacgacgag tcggtgaact cttcgcttgg 24480 tctgcgacca gacggcatct tccaaatagc tggttgtggg agatcttcct tcactcctcg 24540 tcaggctgtc ctgactttgg agagttcgtc ctcgcagccc cgctcgggcg gcatcgggac 24600 tctccagttc gtggaggagt ttactccctc ggtctacttc aaccccttct ccggttctcc 24660 tgggctttac ccggacgagt tcatcccgaa ctacgacgcc atcagtgaag cggtcgacgg 24720 ctacgattaa tgtctaatgg tggcgcggct gagctagctc gactgcgaca cctagaccac 24780 tgccggcgct ttcgctgctt tgctcgggat ctctgcgagt tcatctactt cgagtaccct 24840 gacgaacatc ctcagggacc tgcccacgga gttcggatta ccattgaagg ggctatcgat 24900 tctcacctgc ttcggatctt caccgctcgg ccagtgctag ttgagcgcaa ccagggcgac 24960 accaccatct ccctctgctg catttgtgac aaccccggat tgcatgaaag cttttgttgt 25020 cttctttgta ctgagtataa taaaagctga aattagagac tactccggac tctcttgtcg 25080 tctgaacaac accaaccaga cccttcactt cagcgggaac cagactactc ttcactgtaa 25140 ggcttataac tataagtatc ttacttggat atacaaagga acaccgtttg ctgtggtaaa 25200 caggtgctcc aacgacggtg ttctcctcac cttcctaggc aacttctcca actttacctt 25260 ttctgttcgc agaaacaagc ttaccctcct tcagccctac tttcctggga tctatacctg 25320 cctcagtgga ccttgcaacc acacttttca cctgattgaa aactctaccc ttaccttccc 25380 agcgccaatc cctactaaca gctcggagtc caactcttcc attaccgctg atactaacac 25440 tcctaaaacc ggaggtgagc tccgcagcct tcccccggct gcagataacc cttgggtggt 25500 agcgggattt gtagcgctag gaatagttgc gggtgggctc gcgttcgtcc tctgctacct 25560 ataccttacc tgctgctcat atttagtagt actgtgctgt tggtttagaa aatgggggcg 25620 ctactaatca cacttgcttt actttcgctt ttgggtctga gctcggctaa tagcgagaaa 25680 ccaagctgtc tagaaacaaa ctctccagac tgtgtggttc ctcatgggct ctcagaccca 25740 gctgatgatc catgcttaac ttttgaccca gaaaaaaact gctcggtgac tatgcagccc 25800 tatgcttaca tgtgcacatc tgttataaag tgcggatggg gctgtaaacc gattgaaatt 25860 acccacaaag gcaaaacctg gaataatagt ttgtttaaca catggcagcc tggagacgag 25920 cagtggtata cggccggcca ctggtggaga tgactgaccc catggaaaac tcctctgcca 25980 acgacctgga catggacggc cgttcatctg agcagcgact ggtccagatg cgcattcgcc 26040 agaagcagga acgcgccgcc agagagctca aggatgccat tgaaattcac ctgtgcaaga 26100 agggcatctt ttgcttggtt aagcaagcaa agatttctta tgaaatcact gacaacgacc 26160 accgcctgta ttatgagctc ggtccacagc ggcagaaatt cacctgcatg gttggagtca 26220 accccatagt catcactcag caggctgcag aaattaaagg gtgcatccac tgttcctgtg 26280 attcccaaga atgcgtccac accatagtca agaccctctg cggccttcga gatcttcttc 26340 caatgaacta accccttccc ccaacccaat aaaacattgg ttttaatcat aataaaaaat 26400 cacttacttt aaatctgaaa cagtgtctcc gtccaagttt tcttgtagca ccacttcact 26460 cccctcttcc cagctctggt actgcaagcc ccggtgggct gcaaactttc tccacacctt 26520 aaaagggatg tcaaattcct cttgtccaac aatcttcatt gtctcttcct agatgtccac 26580 aaagcgcgcg cgggtggaag atgactttga ccctgtctac ccatacgatg ctgagctggc 26640 accgtctgta cccttcatcg cccctccctt cgtttcgtca gacggatttc aagaaaaacc 26700 cctgggagtt ctgtccctaa gactagccaa cccagtcact actaaaaatg gggaactcac 26760 acttaaactg ggagatgggg tgggcataga ctcagatgga aacctcacag cacagacagt 26820 tactaaagca acatcccccc ttactgtttc caataacgca attgcactta acatggacaa 26880 acctttttac agtagcaatg gaaaactatc cttacaagtt acatcaccat taaagatagt 26940 cgactcttta aatacattgg ctattggcta tgggcaaggc ttaggactaa acaatagtgc 27000 tcttgctgtg caattagcat ctccccttac atttgacagc aacagcaaaa ttaaaataaa 27060 tttgggaagc gggccattaa aaattaatgc gaataaactg tcaattaact gcctaagggg 27120 tgtatatgta acaactgacg gaacttccat tgaaacaaat ataagctggg caaaaggaat 27180 gaggtttgaa ggtaatgcca tggctgtaaa cgttgacagc accaaaggtc tacaatttgg 27240 cactaccagc acagaatcag gagtcactaa cgctttccct atccagttaa agattggatc 27300 tggtcttagt tttgacagca caggagcact tgtagcttgg gataaggata atgacaagct 27360 tacactgtgg acaaccgctg acccatcacc taattgtacc atatatacag acaaggatgc 27420 taaacttaca ctttgtctta caaaatgtgg cagtcaaata ctaggcagtg tttcagtact 27480 ggctgttaaa gctggaaccc tacagccaat cagtgaaaaa ataggtactg ctttggtttc 27540 actaaaattt aataacaacg gtgtattgtt aagcaactcc acattaagta atgaatactg 27600 gaactacagg aagggagatg tcacaccagc cgaagcctat actaatgctg tgggttttat 27660 gccaaacatc aaggcatatc ctaaaaacac aaactctgcc tcaaaaagcc acattgtagg 27720 acaagtgtac cttaatggag atgaaactaa accaatgcat ttaatcatta catttaatga 27780 aaccagtgat gaaacatgca catattccat aacgttccaa tggaaatgga acattggaac 27840 atacaccagc gacacccttg caacaagctc ctttaccttt tcttacattg cccaagaata 27900 aaaactgcag acaacaataa agtttaaatg ttttatttaa acagtttcac agaaccctag 27960 tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 28020 ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 28080 tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 28140 agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 28200 cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 28260 tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 28320 aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 28380 gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 28440 agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 28500 tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 28560 gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 28620 cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 28680 agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 28740 agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 28800 cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 28860 ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 28920 ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 28980 cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 29040 gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 29100 acgtagtcat tctcgtactt tgagtggcaa aaccttgctc tcgaacagca cacgtctcgt 29160 cgcctcctgt cccttctctt cgccttttca gtgtgatagt tgtaatacag ccattcacga 29220 agctcagtca gaagatcttc agcgtctgtt gtcaaaaaca atccatccaa tctgattgct 29280 ttcaaaacat cacaaacagt cgaataagcc aaacccatcc aggcaatgca attattttgg 29340 ttatccacaa tgggaggggg cggaagacat ggaagaggca taattaattt tttaatccaa 29400 tcgatcacgc agcacttcaa aatgaagatc gcgaaggtga cacctttcac ccccactgtg 29460 ttgatgaaaa ataacagcca agtcaaaatt gatgcggttt tcaaggtgct cgactgtagc 29520 atcaagcaga gcttccacac gcacgtccac aaataacaga atagcaaaag cgggaggagg 29580 aagtaaatcc tcaatcatca tagtacagtc catcaccatc cctaaataat tttcatcctt 29640 ccagccttgg actatatttt taaactgctc ttgtaaatcc aaaccacaca tgtggaaaag 29700 ttcccaaaga gctccctcaa ctaccattct taagcacacc ttcatagtga caaaatatct 29760 tgttcctctg tcacctgcag caaattacaa agtccaatat taggatctat gcccagagat 29820 ctaagctcat ccctcaattc caactgtaaa aaggcttcca gatctgccct aacttgttca 29880 gccagtgggc tccctggaat aagcgtggga gaagccaaac tgcaaaacag acgcatgccg 29940 ccataattac caccagaaaa cactacgtta cagtatgcat gctgattcat tccagtaatt 30000 tcatccagtg tattggatac aaaaaaaggc aagcactctc tcactaattg tattatggag 30060 acattatcac acaggtaaca atttaaaggt tgtggaacaa taatgcagta agtaaccacg 30120 gtgcgctcca acatggttag taatttttag ttctgaaaaa caaaacatac aaaaaattat 30180 atcatactca tttggcgaac tggtggaaaa atgaccctat ctagcacaag gcaagccact 30240 ggatcaccaa tgcgcccctc ataaaacctg tcatcatgat taaaaagcaa caccgaaagc 30300 tcttccctat gtcctgcatg aatgattcta gctgaggaat ataagccagc gcaattagta 30360 tctgttaaag aaaaaaaacg gccaacatag cctctaggaa ttagcacact taatcttaaa 30420 gacattactg ccatccccct tggatttaag gtaaaattta caggagcata gaaaatatac 30480 tgatttccct cctgcacagg cagcatagca ccaggtccct ctaaaaacac acacaaagct 30540 tctgcagcca tagcttaccg cgcaaaccag gcacagcagt gagctaaaag gacaaagctc 30600 taactcacta gccaacctgg cgcacaatat atagttagtc cttacactga cgtaaccgac 30660 caaagtctaa aaaccccgcc aaaaatacac acacgcccaa aaaacgcccc gtgagtcaaa 30720 aaacagtttc acttcctcgt tacacccaaa acgtcgtcac ttccggattc ccacggttcg 30780 tcacttccgg agctccttgc ttaattaacc ccgcccaaaa cgtcatcgtc cgcgtcacgc 30840 cgccccgccc cgcgaccgtt gaccccgggc caatcaccgc acatcccgca aaattcaaac 30900 tcgtctaatt tgcatattgg cacactgccc atataaggta tattattgat gatg 30954 <210> 14 <211> 30615 <212> DNA <213> Artificial Sequence <220> <223> BLY6.dE1.dE3.5IXP Ad vector genome <400> 14 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg gtactgaaat gtgtgggcgt 480 ggcttaaggg tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt 540 gcagcagccg ccgccgccat gagtgggagc gcttcctttg aagggggcgt ctttagccct 600 tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat ggggtctaca 660 gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc tactatgact 720 tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa cactgttcgg 780 gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac taccccaaat 840 aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct ggagtctttg 900 acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca gactcaagct 960 gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat aaaccgagac 1020 tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat atgccctgga 1080 ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat agaggtggga 1140 ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc tccactgcag 1200 agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc gttgggcatg 1260 atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct tggtgtaagt 1320 gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt gcattttgga 1380 ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca tgttatgcaa 1440 gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt tggaggggaa 1500 agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc actcatccat 1560 aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg gatctgacac 1620 atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact tggggcggag 1680 ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc cttcacatat 1740 ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg gagcgatgaa 1800 gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc taagcagctg 1860 tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca gatggtagtt 1920 tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca tcatttccct 1980 cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca gggaaaggag 2040 ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg gcattttgga 2100 gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt ctatggcatc 2160 tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta tggaaccagg 2220 cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt ccgagtcagg 2280 gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag ggtgcgcttc 2340 aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc ggccaggtag 2400 caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc gcgcagctta 2460 cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata gagttttggg 2520 gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca gacggtttcg 2580 cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt tccgccatgt 2640 tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg ggtgacaaag 2700 aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg cgtgccgcgg 2760 tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca ggccagcaca 2820 aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac tttttccaag 2880 gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt gtaagtgtat 2940 gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct ctgctcgtcc 3000 tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta ttccctctcg 3060 aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga ggatttgata 3120 ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc agaaaagaca 3180 atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga aagcagtttg 3240 gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc ggctatgttg 3300 agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt taattcatct 3360 gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac actggttgcc 3420 acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg agaacagaaa 3480 gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt gaaaatccca 3540 ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga catttgccat 3600 tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg catgggatgg 3660 gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc ttccagaatc 3720 cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata atcatagagt 3780 tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt ttctgctctg 3840 tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct ttggaagatg 3900 ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata ggagtcttgc 3960 agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc gagggtctct 4020 ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt gagaaggtat 4080 tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga acggtaagaa 4140 cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc cacggggagt 4200 gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt gtccctgacc 4260 atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg ctcccacagt 4320 tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac atcgttgaat 4380 aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg aggcacttct 4440 gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt gatgttgtga 4500 cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag ttttttaagc 4560 tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc ccagtcaggc 4620 aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc tgtttgtaag 4680 cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt aacacagtaa 4740 aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag gtcgtgggcg 4800 aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg gacaagctgc 4860 ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa gagcctttct 4920 gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt ggaggaatgg 4980 ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg cttgtgcttg 5040 tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat gagctgtacc 5100 tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg catctcgtgt 5160 tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt catgctgacg 5220 agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc gaggacgaga 5280 gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc agtagggaga 5340 gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag atgatatttg 5400 atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg cccctggggt 5460 gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg catggtaagg 5520 tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt tctggacctg 5580 gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg tactgcgccc 5640 tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga cgcctctggg 5700 tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa tcaatttcgg 5760 tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg tcttggtagg 5820 cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg cccgctcgtt 5880 ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat gcattcatgc 5940 ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg gcgcgcatga 6000 ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg cagagacgct 6060 ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac atgatccagc 6120 gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg gcttcgtaaa 6180 agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac tcctcctcca 6240 gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct cccgggatct 6300 cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact tcctcctcag 6360 gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc agacgatcga 6420 tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg gcgcgcccgt 6480 cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg tgactgggag 6540 ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc cctgtaggga 6600 ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc tgaacgaagg 6660 cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg cgggctgctg 6720 agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt ctgagacggc 6780 ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc aggcggtcgg 6840 ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct tgcatgagtc 6900 gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt ccgtaccctc 6960 tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg gcttgctgca 7020 cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag gcccccgtgt 7080 tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt cctggtcgta 7140 cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa tcgttgcagg 7200 ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg tagagaggcc 7260 atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg tggtatccgt 7320 agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc ggaaactctc 7380 gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc acggtctggc 7440 ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa gcgttgagcg 7500 gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac cctggttcga 7560 gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta ttggcaatcc 7620 cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg tttttcaggg 7680 catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt ggctcgcgcc 7740 cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt cgagcctgca 7800 gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg tttctaagac 7860 cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt tttgtttttg 7920 ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc agcagcaggc 7980 tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg ctgtaagcgg 8040 ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg agggattggc 8100 aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa aggacgttcg 8160 cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg agcccgagga 8220 gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg atcgaaaacg 8280 ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc ccgctcgcgc 8340 tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg aggaaagcaa 8400 cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg aggtgaccct 8460 gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca ctagcaaacc 8520 cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg cattcagaga 8580 ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg tgaatatcct 8640 gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg cggcgatcaa 8700 ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa ccccttacgt 8760 gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc tgaaagtgct 8820 aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg cggtgagcgc 8880 cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac gagccctgac 8940 gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc attggctgcc 9000 tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag tagtagacga 9060 ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt tttgctagat 9120 ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt ccggcattaa 9180 ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga cccgcaaccc 9240 cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg aggccgtggt 9300 gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg ccctggtgga 9360 gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc tcgagcgcgt 9420 ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga ccgacgtgcg 9480 cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt ccatggtagc 9540 gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg aagactatac 9600 caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg aggtgtacca 9660 gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag tgaacttaag 9720 ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg gcgacagggc 9780 gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg tgtccccctt 9840 cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa acttgtatcg 9900 cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca ctaatgtgag 9960 tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact ttctgctgac 10020 caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg aacggatctt 10080 gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga cccctagcgc 10140 cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca acagaccatt 10200 cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg attacttcac 10260 caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg gcgagtatga 10320 catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg cgttctcgcc 10380 taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct cctccgtgct 10440 gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct ttcccagtct 10500 gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc gccctcgctt 10560 gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg aaaagaactt 10620 ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga cctatgccca 10680 ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca gacgccagcg 10740 ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg acagcagcgt 10800 gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc gtccccgtat 10860 tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat ggcgaccagc 10920 gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg ctaggcggat 10980 cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag caggtggcgg 11040 cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac ctggcaccta 11100 ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac accacccggt 11160 tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag aacgaccaca 11220 gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag gccagcaccc 11280 agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc atcatgcaca 11340 ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct agggtgatgg 11400 tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt agtcaggaca 11460 tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct gccaccatga 11520 ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt agacagaatg 11580 gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg cttggttggg 11640 acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc catcctgaca 11700 tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt aatctgctag 11760 gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag gatctggagg 11820 gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag gaagataata 11880 caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa gctgtggtag 11940 atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga gatacatttg 12000 ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt ctgtccaccg 12060 atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat agtaaggaca 12120 ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg tacctggcat 12180 acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc acctcggatg 12240 tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa gatccagtca 12300 cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag ctgctcccag 12360 tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc cgccagtaca 12420 cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc cgcccgcccg 12480 cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 12540 cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc agacgccgca 12600 cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt tcaagccgca 12660 ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg gggcctgcgc 12720 acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc tgtgcgcgtg 12780 cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac taggaccacc 12840 gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc cgcagccgca 12900 cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg cgcctatgct 12960 cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc agccatgcga 13020 gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat gcgtagagcg 13080 gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc ggctacggcg 13140 gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta ttgggtgcgc 13200 gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac ttagaagata 13260 ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa ttcaaggaag 13320 agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat gaaaaaaagc 13380 cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat gatgggctgg 13440 tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg cgcaaagtgt 13500 ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc tccagcaccg 13560 tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag caggcggctg 13620 atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag gaagaggcat 13680 tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg accctgcaac 13740 aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat ctgtatccca 13800 ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag aaaatgaaag 13860 tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg gcgcccggtt 13920 tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt caaaccgaac 13980 ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg cccgcgcctg 14040 ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc aacctgataa 14100 tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc ggcactcgct 14160 actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc cgtcgccgcc 14220 gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac cgtgatgggc 14280 gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc atttaacaac 14340 tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt gctggctacc 14400 gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg cgtcgccacc 14460 ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct ttgattccca 14520 tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg caggcctcgc 14580 agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga ctctgacgct 14640 cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt catccctggc 14700 tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca acagccaact 14760 gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt ttgggtccac 14820 tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc tgagacaaaa 14880 gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg gaatcaatgg 14940 ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc tggacccggc 15000 tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt cattggaaaa 15060 gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca aggtggatga 15120 gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc ccattatgcc 15180 tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg ctacattgga 15240 cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca ccgccgtgca 15300 acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact ggcagagcac 15360 tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct gctattgatt 15420 aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg ccgcccgccg 15480 tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag atggccaccc 15540 catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct tcggagtacc 15600 tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat ctggggaaca 15660 agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt agccagcgcc 15720 tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac aaagtcagat 15780 acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac tttgacatca 15840 ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc tacaactccc 15900 tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac aatggtcaag 15960 gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac attgacaaaa 16020 atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt tatgcagaca 16080 aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc gaaaactatt 16140 atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt tccttcgcta 16200 gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag ggtcaacagt 16260 ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc ggaggcaatg 16320 gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta aatctggaaa 16380 ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc gaggccaatt 16440 taactcagca agccatggct aacagaccca actatattgg gtttagagat aactttattg 16500 gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa gcatcccagc 16560 taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag ctattacttg 16620 actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg gacagctatg 16680 atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca aactattgct 16740 tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca aaagaggata 16800 ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata tgcaagggca 16860 acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc ctttactcca 16920 acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg acactcccta 16980 ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc ctggtggatg 17040 cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc aaccctttca 17100 accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac ggccgatacg 17160 tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg ctgcttctcc 17220 caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc ctgcagagtt 17280 cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc atcaatctct 17340 atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc atgctgcgca 17400 atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg ctttacccca 17460 tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg gctgccttca 17520 ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga tcagggttcg 17580 atccctactt cgtctactca ggctccattc catacctgga tggaactttc taccttaacc 17640 acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca ggcaatgaca 17700 ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa gggtacaatg 17760 tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc cactacaaca 17820 ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac tccttcttca 17880 gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa gactacatgg 17940 cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc gcgcccacca 18000 tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc aagactgcag 18060 tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc atccccttct 18120 ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg ctttatgcca 18180 actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag tccacccttc 18240 tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac cgcggcgtca 18300 tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca taagcgcctt 18360 gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat ccaatgagca 18420 agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg gaacctttga 18480 caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg tcaacacggc 18540 agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc gctccaatac 18600 ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga tctatcaatt 18660 cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat gcatcactct 18720 ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc tcttctgctg 18780 catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca accccaccat 18840 gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag agcccaccct 18900 gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt actttcgttc 18960 taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata acatgtaaac 19020 caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg aggctctggg 19080 atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc agatggcagg 19140 gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac cagcctggga 19200 actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag agcgccaagc 19260 aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc gcgggagttg 19320 cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac gctcgccagc 19380 acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat tccaaagggg 19440 gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg gttgcaatca 19500 caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg gtaaacagcc 19560 ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc ggtgtagaac 19620 actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt cacacagcag 19680 cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg ggtgatcttg 19740 gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac atccatctca 19800 atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt gccttcataa 19860 tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt atgggggatc 19920 tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc cagggtcttt 19980 atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg ctggcagatg 20040 cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa ctcattatcc 20100 agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca ggcagaaaca 20160 atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg gtcatcactg 20220 ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg cgggtagctg 20280 aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg actgatgtct 20340 tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc tggaggactc 20400 tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac ttggatctgg 20460 ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt aggcagaggt 20520 ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc ccttccgcgt 20580 tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc cattgtgttc 20640 tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc atccaccacc 20700 acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc cgctaccacc 20760 accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc tatggaagat 20820 attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca tgatgaggat 20880 ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga ggaagaggca 20940 gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga cgtgctgctt 21000 aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg ctccgaggtg 21060 ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc gcctcgcaag 21120 ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa cttctatcca 21180 gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa ccaaaagatc 21240 cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt ggggcccggc 21300 gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga gggtctgggc 21360 aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg tgaacaccac 21420 agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa acgcagcatc 21480 gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat gagctcggtc 21540 atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa catgcaggac 21600 cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc tcgctggcta 21660 ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc agtggtcctg 21720 atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac cctgcgcaag 21780 cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc gtgcaagatc 21840 tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga aaaccggctg 21900 gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta tatccgcgac 21960 tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg gcaacagtgc 22020 ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct taaatctctg 22080 tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat cttccccgag 22140 cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag catgctacaa 22200 aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg ctgcgcactg 22260 ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg gagccactgc 22320 tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga agatgttagt 22380 ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca ccgctccctc 22440 gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga gttgcaaggt 22500 cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac tccggggcta 22560 tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta tgagatcagg 22620 ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt catcacccag 22680 ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt tttgctgaaa 22740 aagggacacg ggatctatct agacccccag accggtgagg agctgaatac acgcttccct 22800 caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg aggagctgga 22860 agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag aggaggacag 22920 cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag ccgccgccag 22980 accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta ccacctccgc 23040 tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg gtcgcttcca 23100 gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt cctggcgggg 23160 gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat cctttgccag 23220 acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc attactaccg 23280 tcacctccac agcccctact accagcagca agagacagca gaggaaacca gcggcaactc 23340 cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt gcaggcggac 23400 tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg atctttccca 23460 ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa gtaaaaaaca 23520 gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac caacttcagc 23580 gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt 23640 agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc cacacctgtg 23700 cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc cccagatggg 23760 attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca gcgccggtcc 23820 cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac tcctagaaca 23880 gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc ccgccgccct 23940 ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg cccaggccga 24000 agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt gtcgtcaccg 24060 gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc tcaacgacga 24120 gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag ctggttgtgg 24180 gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt cctcgcagcc 24240 ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct cggtctactt 24300 caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga actacgacgc 24360 catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc tgagctagct 24420 cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga tctctgcgag 24480 ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg agttcggatt 24540 accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg gccagtgcta 24600 gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga caaccccgga 24660 ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg aaattagaga 24720 ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact tcagcgggaa 24780 ccagactact cttcactgta aggcttataa ctataagtat cttacttgga tatacaaagg 24840 aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca ccttcctagg 24900 caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc ttcagcccta 24960 ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc acctgattga 25020 aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt ccaactcttc 25080 cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc ttcccccggc 25140 tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg cgggtgggct 25200 cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag tactgtgctg 25260 ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct tttgggtctg 25320 agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga ctgtgtggtt 25380 cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc agaaaaaaac 25440 tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa gtgcggatgg 25500 ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag tttgtttaac 25560 acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag atgactgacc 25620 ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct gagcagcgac 25680 tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc aaggatgcca 25740 ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca aagatttctt 25800 atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag cggcagaaat 25860 tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca gaaattaaag 25920 ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc aagaccctct 25980 gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa taaaacattg 26040 gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc cgtccaagtt 26100 ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc cccggtgggc 26160 tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa caatcttcat 26220 tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg accctgtcta 26280 cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct tcgtttcgtc 26340 agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca acccagtcac 26400 tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag actcagatgg 26460 aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt ccaataacgc 26520 aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat ccttacaagt 26580 tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct atgggcaagg 26640 cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta catttgacag 26700 caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg cgaataaact 26760 gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca ttgaaacaaa 26820 tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa acgttgacag 26880 caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta acgctttccc 26940 tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac ttgtagcttg 27000 ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac ctaattgtac 27060 catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg gcagtcaaat 27120 actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa tcagtgaaaa 27180 aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt taagcaactc 27240 cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag ccgaagccta 27300 tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca caaactctgc 27360 ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta aaccaatgca 27420 tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca taacgttcca 27480 atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct cctttacctt 27540 ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat gttttattta 27600 aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac acagagtaca 27660 cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca gacatattct 27720 taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg atattaataa 27780 actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc acaggctgct 27840 gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac atgggggtag 27900 agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga ataaactgct 27960 gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca gcgatgattc 28020 gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc ctgatctcac 28080 ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc ccacagtgca 28140 aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca tcataccaca 28200 agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac attacctctt 28260 ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta aacatggcgc 28320 catccaccac catcctaaac cagctggcca aaacctgccc gccggctata cactgcaggg 28380 aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg atcatcatgc 28440 tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc ctcaggatta 28500 caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga atcagcgtaa 28560 atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc aaagtgttac 28620 attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc tcaaaaggag 28680 gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt ggtcgtagtg 28740 tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca aaaccttgct 28800 ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc agtgtgatag 28860 ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt tgtcaaaaac 28920 aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc caaacccatc 28980 caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca tggaagaggc 29040 ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat cgcgaaggtg 29100 acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat tgatgcggtt 29160 ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca caaataacag 29220 aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt ccatcaccat 29280 ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct cttgtaaatc 29340 caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc ttaagcacac 29400 cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca aagtccaata 29460 ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa aaaggcttcc 29520 agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg agaagccaaa 29580 ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt acagtatgca 29640 tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg caagcactct 29700 ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg ttgtggaaca 29760 ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta gttctgaaaa 29820 acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa aatgacccta 29880 tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct gtcatcatga 29940 ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct agctgaggaa 30000 tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata gcctctagga 30060 attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa ggtaaaattt 30120 acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc accaggtccc 30180 tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca ggcacagcag 30240 tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata tatagttagt 30300 ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca cacacgccca 30360 aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa aacgtcgtca 30420 cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac cccgcccaaa 30480 acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg ccaatcaccg 30540 cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc catataaggt 30600 atattattga tgatg 30615 <210> 15 <211> 3586 <212> DNA <213> Artificial Sequence <220> <223> BLY6 vector left end <400> 15 caattgattt aaatcatcat caataatata ccttatatgg gcagtgtgcc aatatgcaaa 60 ttagacgagt ttgaattttg cgggatgtgc ggtgattggc ccggggtcaa cggtcgcggg 120 gcggggcggc gtgacgcgga cgatgacgtt ttggggagga ggagctatgt tgcaagtaat 180 cgtgggaaat gcgacgtaaa acgaggtgga gtttaaacac ggaagtagac aattttcccg 240 cgctgtttga caggaaatga tgtgtttttg ggcggatgca agtgaaaatt ctccattttc 300 gcgcgaaaac taaatgagga agtgaaattc tgagtaattc tgaggttatc acagggcgga 360 gtatttaccg agggccgagt agactttgac ccattacgtg gaggtttcga ttactctatt 420 tttcacctaa atttccgcgt actgtgtcaa agtccgtgtt tttacgcgat cgcgatggcc 480 tgtgtttgag cataatgtac tgaccaggtg taacgttcat ctgggtggtc gtagaggaat 540 gttcatgcca taccaatgca attttaatca tgtgaggatc ttgatggagc cgcaagcgtt 600 ttccagagtc agcttgactg gaatctttga catgtgtgtg gaagcatgga agatcttaag 660 atatgatgat accaaatcca gatgccgcgc atgcgagtgc gggggcaggc atgccaggtt 720 ccaacctgta tgtgtggagg tgaccgagga gctgagacca gatcatttgg tgctgacctg 780 cactggtgcg gagttcggtt ccagtggtga agaaactgat taaagtgagt agtgggatgt 840 tataaaagtg accataaggt gatgtgagat ggacaaattt ggtaattttt atgtattttt 900 gtcttgcagc catgagtggg agcgcttcct ttgaaggggg cgtctttagc ccttatctga 960 cggggcgtct gcctcattgg gctggagtgc gtcagaatgt gatggggtct acagtggatg 1020 gaagacctgt tcagcctgct aattcttcta ctctgactta tgctactatg acttcctcgc 1080 ctttggatgc agctgcagct gctgccgctt ctgctgccgc caacactgtt cgggggatgg 1140 ccttggagat ggggtattat ggaactgtag tggccaacac cactacccca aataacccca 1200 cagccttgaa tgaggacaag ctgctagttc tcatgtccca gctggagtct ttgacccaac 1260 gcctgggcga tctagctcag caggtgtccc agctgaagga gcagactcaa gctgcaatta 1320 ccactgcgag gggaaattaa aaaaattcaa agaatcaata aataaaccga gactttgttg 1380 attttaaagt gtgtcattct ttatttaatt tttcgcgcgc gatatgccct ggaccaccgg 1440 tctctatcat tgaggacacg gtggatcttt tctagaaccc gatagaggtg ggattggatg 1500 ttgaggtaca tgggcataag accatctttg gggtgtagat agctccactg cagagcctca 1560 tgctccgggg tggtgttgta tataacccag tcatagcatg ggcgttgggc atgatgttgc 1620 acaatatctt taaggaggag actaatggcc actgggagac ccttggtgta agtgtttaca 1680 aatctattaa gctgggacgg gtgcatccga ggtgagataa tgtgcatttt ggattggatt 1740 tttagattgg caatgtttcc ccctagatct ctcctgggat tcatgttatg caagaccact 1800 agaacagtgt atccggtgca cttagggaat ttgtcatgaa gtttggaggg gaaagcatga 1860 aaaaatttag acacaccctt gtgtcctccc aagttctcca tgcactcatc cataataatg 1920 gcaatgggcc catgggcggc ggcacgggcg aacacgttcc tgggatctga cacatcatag 1980 ttgtggtctt gggtcaggtc atcataagcc attttaataa acttggggcg gagggtgcca 2040 gattggggga tgaatgttcc ctcgggcccc ggaacatagt ttccttcaca tatttgcatt 2100 tcccaggctt ttagttcaga gggggggatc atgtccacct gtggagcgat gaagaagacg 2160 gtctcggggg cgggggtgat taagtgggag gacagcaagt tcctaagcag ctgtgacttg 2220 ccacacccag tgggaccgta gatgacccct ataacaggtt gcagatggta gtttagggaa 2280 agacagctgc cgtcctctcg caggaggggg gcgacctcgt tcatcatttc cctcacatgc 2340 atgttttccc gcacaagttc cgataggagg cgctctccac ccagggaaag gagttcttga 2400 agagatgaga aatttttcaa gggttttaag ccatcagcca tgggcatttt ggagagggtt 2460 tgttgcaaga gttcaaggcg gtcccagagt tcggtgatgt gttctatggc atctcgatcc 2520 agcatacttc ctcgtttctg gggttgggac ggctgcggga gtatggaacc aggcgatggg 2580 cgtccagcgc tgccagtgtc cggtccttcc acggtcgcag cgtccgagtc agggtcgttt 2640 ccgtcacggt gaaggggtgc gcgcctggct gggcgcttgc gagggtgcgc ttcaggctca 2700 tcctgctcgt ggagaaccgc tgccgttctg cgccctgtgc atcggccagg tagcaattaa 2760 ccatgagttc gtagttgagc gcctctgccg cgtggccttt ggcgcgcagc ttacctttgg 2820 aagtcttctg acaggtggga cagtagagac acttgagagc atagagtttt ggggctagaa 2880 agaccgattc tggggagtat gcatcggccc cacaggaggc gcagacggtt tcgcattcca 2940 ccagccatgt aagatcgggc tcgttggggt caaaaacaag ttttccgcca tgttttttga 3000 tgcgtttctt acctttgctt tccatgagtt cgtgcccccg ttgggtgaca aagaggctgt 3060 ccgtgtcccc gtagactgac tttatgggcc tgtcctcgag cggcgtgccg cggtcctctt 3120 cgtagaggaa ctcggaccac tctgagacga aagcacgtgt ccaggccagc acaaaggagg 3180 ctatatggga ggggtagcga tcgttgtcaa ccaaggggtc tactttttcc aaggtgtgta 3240 aacacatgtc cccttcttcc acatccagga aggtgattgg cttgtaagtg tatgccacgt 3300 gacctggggt cccagacggg ggggtataaa agggggcggg tctctgctcg tcctcactgt 3360 cttccggatc gctgtccagg agcgccagct gttgaggtag gtattccctc tcgaaggcgg 3420 gcataacctc cgcactcagg ttgtcagttt ctaggaacga ggaggatttg atattgacag 3480 tgcctgccga gatgcctttc atgagactgt cgtccatttg gtcagaaaag acaatctttt 3540 tgttatcaag tttggtggcg aaggatccga tcgatcgatc catatg 3586 <210> 16 <211> 4138 <212> DNA <213> Artificial Sequence <220> <223> BLY6 vector right end <400> 16 ggatcccccc ttactgtttc caataacgca attgcactta acatggacaa acctttttac 60 agtagcaatg gaaaactatc cttacaagtt acatcaccat taaagatagt cgactcttta 120 aatacattgg ctattggcta tgggcaaggc ttaggactaa acaatagtgc tcttgctgtg 180 caattagcat ctccccttac atttgacagc aacagcaaaa ttaaaataaa tttgggaagc 240 gggccattaa aaattaatgc gaataaactg tcaattaact gcctaagggg tgtatatgta 300 acaactgacg gaacttccat tgaaacaaat ataagctggg caaaaggaat gaggtttgaa 360 ggtaatgcca tggctgtaaa cgttgacagc accaaaggtc tacaatttgg cactaccagc 420 acagaatcag gagtcactaa cgctttccct atccagttaa agattggatc tggtcttagt 480 tttgacagca caggagcact tgtagcttgg gataaggata atgacaagct tacactgtgg 540 acaaccgctg acccatcacc taattgtacc atatatacag acaaggatgc taaacttaca 600 ctttgtctta caaaatgtgg cagtcaaata ctaggcagtg tttcagtact ggctgttaaa 660 gctggaaccc tacagccaat cagtgaaaaa ataggtactg ctttggtttc actaaaattt 720 aataacaacg gtgtattgtt aagcaactcc acattaagta atgaatactg gaactacagg 780 aagggagatg tcacaccagc cgaagcctat actaatgctg tgggttttat gccaaacatc 840 aaggcatatc ctaaaaacac aaactctgcc tcaaaaagcc acattgtagg acaagtgtac 900 cttaatggag atgaaactaa accaatgcat ttaatcatta catttaatga aaccagtgat 960 gaaacatgca catattccat aacgttccaa tggaaatgga acattggaac atacaccagc 1020 gacacccttg caacaagctc ctttaccttt tcttacattg cccaagaata aaaactgcag 1080 acaacaataa agtttaaatg ttttatttaa acagtttcac agaaccctag tattcaacct 1140 gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg ccttaaaaag 1200 catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg tttcctgtcg 1260 agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta agttcatgtc 1320 gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa cgggcggcga 1380 aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga tagggcggtg 1440 gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc aggaatacaa 1500 catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc gccttgtcct 1560 ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc agcacagcac 1620 cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca tggcggggac 1680 cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc gacccctcat 1740 aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca cctcccggta 1800 ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc agctggccaa 1860 aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac agtggagagc 1920 ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg cacaacacag 1980 gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa ccatatccca 2040 gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac ctcgcacgta 2100 actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat cctccagtat 2160 ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg gagtgcgccg 2220 agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg acgtagtcat 2280 tctcgtactt tgagtggcaa aaccttgctc tcgaacagca cacgtctcgt cgcctcctgt 2340 cccttctctt cgccttttca gtgtgatagt tgtaatacag ccattcacga agctcagtca 2400 gaagatcttc agcgtctgtt gtcaaaaaca atccatccaa tctgattgct ttcaaaacat 2460 cacaaacagt cgaataagcc aaacccatcc aggcaatgca attattttgg ttatccacaa 2520 tgggaggggg cggaagacat ggaagaggca taattaattt tttaatccaa tcgatcacgc 2580 agcacttcaa aatgaagatc gcgaaggtga cacctttcac ccccactgtg ttgatgaaaa 2640 ataacagcca agtcaaaatt gatgcggttt tcaaggtgct cgactgtagc atcaagcaga 2700 gcttccacac gcacgtccac aaataacaga atagcaaaag cgggaggagg aagtaaatcc 2760 tcaatcatca tagtacagtc catcaccatc cctaaataat tttcatcctt ccagccttgg 2820 actatatttt taaactgctc ttgtaaatcc aaaccacaca tgtggaaaag ttcccaaaga 2880 gctccctcaa ctaccattct taagcacacc ttcatagtga caaaatatct tgttcctctg 2940 tcacctgcag caaattacaa agtccaatat taggatctat gcccagagat ctaagctcat 3000 ccctcaattc caactgtaaa aaggcttcca gatctgccct aacttgttca gccagtgggc 3060 tccctggaat aagcgtggga gaagccaaac tgcaaaacag acgcatgccg ccataattac 3120 caccagaaaa cactacgtta cagtatgcat gctgattcat tccagtaatt tcatccagtg 3180 tattggatac aaaaaaaggc aagcactctc tcactaattg tattatggag acattatcac 3240 acaggtaaca atttaaaggt tgtggaacaa taatgcagta agtaaccacg gtgcgctcca 3300 acatggttag taatttttag ttctgaaaaa caaaacatac aaaaaattat atcatactca 3360 tttggcgaac tggtggaaaa atgaccctat ctagcacaag gcaagccact ggatcaccaa 3420 tgcgcccctc ataaaacctg tcatcatgat taaaaagcaa caccgaaagc tcttccctat 3480 gtcctgcatg aatgattcta gctgaggaat ataagccagc gcaattagta tctgttaaag 3540 aaaaaaaacg gccaacatag cctctaggaa ttagcacact taatcttaaa gacattactg 3600 ccatccccct tggatttaag gtaaaattta caggagcata gaaaatatac tgatttccct 3660 cctgcacagg cagcatagca ccaggtccct ctaaaaacac acacaaagct tctgcagcca 3720 tagcttaccg cgcaaaccag gcacagcagt gagctaaaag gacaaagctc taactcacta 3780 gccaacctgg cgcacaatat atagttagtc cttacactga cgtaaccgac caaagtctaa 3840 aaaccccgcc aaaaatacac acacgcccaa aaaacgcccc gtgagtcaaa aaacagtttc 3900 acttcctcgt tacacccaaa acgtcgtcac ttccggattc ccacggttcg tcacttccgg 3960 agctccttgc ttaattaacc ccgcccaaaa cgtcatcgtc cgcgtcacgc cgccccgccc 4020 cgcgaccgtt gaccccgggc caatcaccgc acatcccgca aaattcaaac tcgtctaatt 4080 tgcatattgg cacactgccc atataaggta tattattgat gatgatttaa atcatatg 4138 <210> 17 <211> 4563 <212> DNA <213> Artificial Sequence <220> <223> BLY6 vector middle fragment <400> 17 ggatccatac agggcattgg aaagcagttt ggcaatggag cgcatggttt ggtttttttc 60 tttgtctgcg cgctctttgg cggctatgtt gagttggaca tattcgcggg ccagacattt 120 ccattgtgga aatatggtag ttaattcatc tgggacgatt ctgactttcc agcctctgtt 180 atgcagggta atcagatcca cactggttgc cacttctcct ctaagtggtt cattagtcca 240 gcatagtcgc cccccttttc gagaacagaa agggggtagg ggatctagca tgagttcgtc 300 tggggggtct gcatctatgg tgaaaatccc aggaaggaga tcttcgtcaa aatagctgat 360 ggtggcgggg tcatccagag acatttgcca ttctcgagca gccagagcgc gctcgtaggg 420 gttaagggga gtcccccatg gcatgggatg ggtgagtgca gaagcataca tgccacagat 480 gtcatagaca tagagcggct cttccagaat ccctatgtaa gtgggataac atcgcccccc 540 tctgatgctg gctcgcacat aatcatagag ttcatgtgag ggcgctagaa gacccgagcc 600 caggttggtg cggttgggtt tttctgctct gtagaggatc tggcgaaaga tggcatggga 660 gtttgatgag atggtgggtc tttggaagat gttgaaatgg gcatgaggca gtcccacaga 720 gtcccttatg aagtgagcat aggagtcttg cagtttggcc accagctcgg cggtgaccag 780 cacatccaaa gcacagtagt cgagggtctc tttgatgatg tcatagttag gttccccttt 840 cttttcccac agctcgcggt tgagaaggta ttcttcgcga tccttccagt actcttcgag 900 ggggaacccg tccttgtctg aacggtaaga acccagcatg taaaattgat tgacagcttt 960 gtaggcacaa caccccttct ccacggggag tgagtatgct tgcgcggctt tgcgcagaga 1020 ggtgtgagta agggcgaaag tgtccctgac catgactttg aggaactgat gcttaaagtc 1080 tatgtcatcg caggccccct gctcccacag ttggaagtcc actcgctttt tgtaggcggg 1140 attgggcaaa gcgaaagtaa catcgttgaa taggatcttt ccagccctgg gcatgaagtt 1200 gcgagtaatg cgaaaaggct gaggcacttc tgccctgttg ttgataactt gggcagccaa 1260 gacgatctcg tcaaagccgt tgatgttgtg acccacaatg taaagttcta cgaagcgtgg 1320 gcgtcccttg atgtggggca gttttttaag ctcttcgtag gtcaagtcgt cagggtcagc 1380 gattccatat tgctccaaag cccagtcagg caggtgagga ttagcatgaa ggaaagaggt 1440 ccaaagatcc acggccagag ctgtttgtaa gcggtctctg tactgacgga aatgtcggcc 1500 taccgccatt ttttcaggag taacacagta aaaggtgcgc gggtcctttt cccagcgatc 1560 ccattgaagt tgcaaggcta ggtcgtgggc gaggttgacg agctgttcgt cccccgaaag 1620 tttcatgacc agcatgaaag ggacaagctg cttgccaaag gaccccatcc aggtgtaggt 1680 ttccacatcg taggtgagga agagcctttc tgtgcgagga tgagaaccga tcgggaagaa 1740 ctggatttcc tgccaccagt tggaggaatg gctgttgata tgatggaagt agaactccct 1800 acggcgcgcc gagcattcgt gcttgtgctt gtacagacgg ccacagtact cgcagcgctg 1860 cacgggatgc acctgatgaa tgagctgtac ctggcttcct ttgacaagaa atttcagtgg 1920 gaagttgagg cgtggcgtct gcatctcgtg ttaacccccg taattggccc gccgccctgg 1980 tgtaccagga aactcctgct cccactacag tactacttcc tcgtgacgcc caggccgaag 2040 ttcagatgac taactcaggt gtacagctgg cgggtggtgc caccctgtgt cgtcaccggc 2100 caagaccggg tataaagggc ctggtgatca gaggccgagg tattcagctc aacgacgagt 2160 cggtgaactc ttcgcttggt ctgcgaccag acggcatctt ccaaatagct ggttgtggga 2220 gatcttcctt cactcctcgt caggctgtcc tgactttgga gagttcgtcc tcgcagcccc 2280 gctcgggcgg catcgggact ctccagttcg tggaggagtt tactccctcg gtctacttca 2340 accccttctc cggttctcct gggctttacc cggacgagtt catcccgaac tacgacgcca 2400 tcagtgaagc ggtcgacggc tacgattaat gtctaatggt ggcgcggctg agctagctcg 2460 actgcgacac ctagaccact gccggcgctt tcgctgcttt gctcgggatc tctgcgagtt 2520 catctacttc gagtaccctg acgaacatcc tcagggacct gcccacggag ttcggattac 2580 cattgaaggg gctatcgatt ctcacctgct tcggatcttc accgctcggc cagtgctagt 2640 tgagcgcaac cagggcgaca ccaccatctc cctctgctgc atttgtgaca accccggatt 2700 gcatgaaagc ttttgttgtc ttctttgtac tgagtataat aaaagctgaa attagagact 2760 actccggact ctcttgtcgt ctgaacaaca ccaaccagac ccttcacttc agcgggaacc 2820 agactactct tcactgtaag gcttataact ataagtatct tacttggata tacaaaggaa 2880 caccgtttgc tgtggtaaac aggtgctcca acgacggtgt tctcctcacc ttcctaggca 2940 acttctccaa ctttaccttt tctgttcgca gaaacaagct taccctcctt cagccctact 3000 ttcctgggat ctatacctgc ctcagtggac cttgcaacca cacttttcac ctgattgaaa 3060 actctaccct taccttccca gcgccaatcc ctactaacag ctcggagtcc aactcttcca 3120 ttaccgctga tactaacact cctaaaaccg gaggtgagct ccgcagcctt cccccggctg 3180 cagataaccc ttgggtggta gcgggatttg tagcgctagg aatagttgcg ggtgggctcg 3240 cgttcgtcct ctgctaccta taccttacct gctgctcata tttagtagta ctgtgctgtt 3300 ggtttagaaa atgggggcgc tactaatcac acttgcttta ctttcgcttt tgggtctgag 3360 ctcggctaat agcgagaaac caagctgtct agaaacaaac tctccagact gtgtggttcc 3420 tcatgggctc tcagacccag ctgatgatcc atgcttaact tttgacccag aaaaaaactg 3480 ctcggtgact atgcagccct atgcttacat gtgcacatct gttataaagt gcggatgggg 3540 ctgtaaaccg attgaaatta cccacaaagg caaaacctgg aataatagtt tgtttaacac 3600 atggcagcct ggagacgagc agtggtatac ggccggccac tggtggagat gactgacccc 3660 atggaaaact cctctgccaa cgacctggac atggacggcc gttcatctga gcagcgactg 3720 gtccagatgc gcattcgcca gaagcaggaa cgcgccgcca gagagctcaa ggatgccatt 3780 gaaattcacc tgtgcaagaa gggcatcttt tgcttggtta agcaagcaaa gatttcttat 3840 gaaatcactg acaacgacca ccgcctgtat tatgagctcg gtccacagcg gcagaaattc 3900 acctgcatgg ttggagtcaa ccccatagtc atcactcagc aggctgcaga aattaaaggg 3960 tgcatccact gttcctgtga ttcccaagaa tgcgtccaca ccatagtcaa gaccctctgc 4020 ggccttcgag atcttcttcc aatgaactaa ccccttcccc caacccaata aaacattggt 4080 tttaatcata ataaaaaatc acttacttta aatctgaaac agtgtctccg tccaagtttt 4140 cttgtagcac cacttcactc ccctcttccc agctctggta ctgcaagccc cggtgggctg 4200 caaactttct ccacacctta aaagggatgt caaattcctc ttgtccaaca atcttcattg 4260 tctcttccta gatgtccaca aagcgcgcgc gggtggaaga tgactttgac cctgtctacc 4320 catacgatgc tgagctggca ccgtctgtac ccttcatcgc ccctcccttc gtttcgtcag 4380 acggatttca agaaaaaccc ctgggagttc tgtccctaag actagccaac ccagtcacta 4440 ctaaaaatgg ggaactcaca cttaaactgg gagatggggt gggcatagac tcagatggaa 4500 acctcacagc acagacagtt actaaagcaa catcccccct tactgtttcc aataacgcaa 4560 ttg 4563 <210> 18 <211> 14287 <212> DNA <213> Artificial Sequence <220> <223> BLY6 intermediate plasmid 3 <400> 18 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg atggcctgtg tttgagcata 480 atgtactgac caggtgtaac gttcatctgg gtggtcgtag aggaatgttc atgccatacc 540 aatgcaattt taatcatgtg aggatcttga tggagccgca agcgttttcc agagtcagct 600 tgactggaat ctttgacatg tgtgtggaag catggaagat cttaagatat gatgatacca 660 aatccagatg ccgcgcatgc gagtgcgggg gcaggcatgc caggttccaa cctgtatgtg 720 tggaggtgac cgaggagctg agaccagatc atttggtgct gacctgcact ggtgcggagt 780 tcggttccag tggtgaagaa actgattaaa gtgagtagtg ggatgttata aaagtgacca 840 taaggtgatg tgagatggac aaatttggta atttttatgt atttttgtct tgcagccatg 900 agtgggagcg cttcctttga agggggcgtc tttagccctt atctgacggg gcgtctgcct 960 cattgggctg gagtgcgtca gaatgtgatg gggtctacag tggatggaag acctgttcag 1020 cctgctaatt cttctactct gacttatgct actatgactt cctcgccttt ggatgcagct 1080 gcagctgctg ccgcttctgc tgccgccaac actgttcggg ggatggcctt ggagatgggg 1140 tattatggaa ctgtagtggc caacaccact accccaaata accccacagc cttgaatgag 1200 gacaagctgc tagttctcat gtcccagctg gagtctttga cccaacgcct gggcgatcta 1260 gctcagcagg tgtcccagct gaaggagcag actcaagctg caattaccac tgcgagggga 1320 aattaaaaaa attcaaagaa tcaataaata aaccgagact ttgttgattt taaagtgtgt 1380 cattctttat ttaatttttc gcgcgcgata tgccctggac caccggtctc tatcattgag 1440 gacacggtgg atcttttcta gaacccgata gaggtgggat tggatgttga ggtacatggg 1500 cataagacca tctttggggt gtagatagct ccactgcaga gcctcatgct ccggggtggt 1560 gttgtatata acccagtcat agcatgggcg ttgggcatga tgttgcacaa tatctttaag 1620 gaggagacta atggccactg ggagaccctt ggtgtaagtg tttacaaatc tattaagctg 1680 ggacgggtgc atccgaggtg agataatgtg cattttggat tggattttta gattggcaat 1740 gtttccccct agatctctcc tgggattcat gttatgcaag accactagaa cagtgtatcc 1800 ggtgcactta gggaatttgt catgaagttt ggaggggaaa gcatgaaaaa atttagacac 1860 acccttgtgt cctcccaagt tctccatgca ctcatccata ataatggcaa tgggcccatg 1920 ggcggcggca cgggcgaaca cgttcctggg atctgacaca tcatagttgt ggtcttgggt 1980 caggtcatca taagccattt taataaactt ggggcggagg gtgccagatt gggggatgaa 2040 tgttccctcg ggccccggaa catagtttcc ttcacatatt tgcatttccc aggcttttag 2100 ttcagagggg gggatcatgt ccacctgtgg agcgatgaag aagacggtct cgggggcggg 2160 ggtgattaag tgggaggaca gcaagttcct aagcagctgt gacttgccac acccagtggg 2220 accgtagatg acccctataa caggttgcag atggtagttt agggaaagac agctgccgtc 2280 ctctcgcagg aggggggcga cctcgttcat catttccctc acatgcatgt tttcccgcac 2340 aagttccgat aggaggcgct ctccacccag ggaaaggagt tcttgaagag atgagaaatt 2400 tttcaagggt tttaagccat cagccatggg cattttggag agggtttgtt gcaagagttc 2460 aaggcggtcc cagagttcgg tgatgtgttc tatggcatct cgatccagca tacttcctcg 2520 tttctggggt tgggacggct gcgggagtat ggaaccaggc gatgggcgtc cagcgctgcc 2580 agtgtccggt ccttccacgg tcgcagcgtc cgagtcaggg tcgtttccgt cacggtgaag 2640 gggtgcgcgc ctggctgggc gcttgcgagg gtgcgcttca ggctcatcct gctcgtggag 2700 aaccgctgcc gttctgcgcc ctgtgcatcg gccaggtagc aattaaccat gagttcgtag 2760 ttgagcgcct ctgccgcgtg gcctttggcg cgcagcttac ctttggaagt cttctgacag 2820 gtgggacagt agagacactt gagagcatag agttttgggg ctagaaagac cgattctggg 2880 gagtatgcat cggccccaca ggaggcgcag acggtttcgc attccaccag ccatgtaaga 2940 tcgggctcgt tggggtcaaa aacaagtttt ccgccatgtt ttttgatgcg tttcttacct 3000 ttgctttcca tgagttcgtg cccccgttgg gtgacaaaga ggctgtccgt gtccccgtag 3060 actgacttta tgggcctgtc ctcgagcggc gtgccgcggt cctcttcgta gaggaactcg 3120 gaccactctg agacgaaagc acgtgtccag gccagcacaa aggaggctat atgggagggg 3180 tagcgatcgt tgtcaaccaa ggggtctact ttttccaagg tgtgtaaaca catgtcccct 3240 tcttccacat ccaggaaggt gattggcttg taagtgtatg ccacgtgacc tggggtccca 3300 gacggggggg tataaaaggg ggcgggtctc tgctcgtcct cactgtcttc cggatcgctg 3360 tccaggagcg ccagctgttg aggtaggtat tccctctcga aggcgggcat aacctccgca 3420 ctcaggttgt cagtttctag gaacgaggag gatttgatat tgacagtgcc tgccgagatg 3480 cctttcatga gactgtcgtc catttggtca gaaaagacaa tctttttgtt atcaagtttg 3540 gtggcgaagg atccatacag ggcattggaa agcagtttgg caatggagcg catggtttgg 3600 tttttttctt tgtctgcgcg ctctttggcg gctatgttga gttggacata ttcgcgggcc 3660 agacatttcc attgtggaaa tatggtagtt aattcatctg ggacgattct gactttccag 3720 cctctgttat gcagggtaat cagatccaca ctggttgcca cttctcctct aagtggttca 3780 ttagtccagc atagtcgccc cccttttcga gaacagaaag ggggtagggg atctagcatg 3840 agttcgtctg gggggtctgc atctatggtg aaaatcccag gaaggagatc ttcgtcaaaa 3900 tagctgatgg tggcggggtc atccagagac atttgccatt ctcgagcagc cagagcgcgc 3960 tcgtaggggt taaggggagt cccccatggc atgggatggg tgagtgcaga agcatacatg 4020 ccacagatgt catagacata gagcggctct tccagaatcc ctatgtaagt gggataacat 4080 cgcccccctc tgatgctggc tcgcacataa tcatagagtt catgtgaggg cgctagaaga 4140 cccgagccca ggttggtgcg gttgggtttt tctgctctgt agaggatctg gcgaaagatg 4200 gcatgggagt ttgatgagat ggtgggtctt tggaagatgt tgaaatgggc atgaggcagt 4260 cccacagagt cccttatgaa gtgagcatag gagtcttgca gtttggccac cagctcggcg 4320 gtgaccagca catccaaagc acagtagtcg agggtctctt tgatgatgtc atagttaggt 4380 tcccctttct tttcccacag ctcgcggttg agaaggtatt cttcgcgatc cttccagtac 4440 tcttcgaggg ggaacccgtc cttgtctgaa cggtaagaac ccagcatgta aaattgattg 4500 acagctttgt aggcacaaca ccccttctcc acggggagtg agtatgcttg cgcggctttg 4560 cgcagagagg tgtgagtaag ggcgaaagtg tccctgacca tgactttgag gaactgatgc 4620 ttaaagtcta tgtcatcgca ggccccctgc tcccacagtt ggaagtccac tcgctttttg 4680 taggcgggat tgggcaaagc gaaagtaaca tcgttgaata ggatctttcc agccctgggc 4740 atgaagttgc gagtaatgcg aaaaggctga ggcacttctg ccctgttgtt gataacttgg 4800 gcagccaaga cgatctcgtc aaagccgttg atgttgtgac ccacaatgta aagttctacg 4860 aagcgtgggc gtcccttgat gtggggcagt tttttaagct cttcgtaggt caagtcgtca 4920 gggtcagcga ttccatattg ctccaaagcc cagtcaggca ggtgaggatt agcatgaagg 4980 aaagaggtcc aaagatccac ggccagagct gtttgtaagc ggtctctgta ctgacggaaa 5040 tgtcggccta ccgccatttt ttcaggagta acacagtaaa aggtgcgcgg gtccttttcc 5100 cagcgatccc attgaagttg caaggctagg tcgtgggcga ggttgacgag ctgttcgtcc 5160 cccgaaagtt tcatgaccag catgaaaggg acaagctgct tgccaaagga ccccatccag 5220 gtgtaggttt ccacatcgta ggtgaggaag agcctttctg tgcgaggatg agaaccgatc 5280 gggaagaact ggatttcctg ccaccagttg gaggaatggc tgttgatatg atggaagtag 5340 aactccctac ggcgcgccga gcattcgtgc ttgtgcttgt acagacggcc acagtactcg 5400 cagcgctgca cgggatgcac ctgatgaatg agctgtacct ggcttccttt gacaagaaat 5460 ttcagtggga agttgaggcg tggcgtctgc atctcgtgtt aacccccgta attggcccgc 5520 cgccctggtg taccaggaaa ctcctgctcc cactacagta ctacttcctc gtgacgccca 5580 ggccgaagtt cagatgacta actcaggtgt acagctggcg ggtggtgcca ccctgtgtcg 5640 tcaccggcca agaccgggta taaagggcct ggtgatcaga ggccgaggta ttcagctcaa 5700 cgacgagtcg gtgaactctt cgcttggtct gcgaccagac ggcatcttcc aaatagctgg 5760 ttgtgggaga tcttccttca ctcctcgtca ggctgtcctg actttggaga gttcgtcctc 5820 gcagccccgc tcgggcggca tcgggactct ccagttcgtg gaggagttta ctccctcggt 5880 ctacttcaac cccttctccg gttctcctgg gctttacccg gacgagttca tcccgaacta 5940 cgacgccatc agtgaagcgg tcgacggcta cgattaatgt ctaatggtgg cgcggctgag 6000 ctagctcgac tgcgacacct agaccactgc cggcgctttc gctgctttgc tcgggatctc 6060 tgcgagttca tctacttcga gtaccctgac gaacatcctc agggacctgc ccacggagtt 6120 cggattacca ttgaaggggc tatcgattct cacctgcttc ggatcttcac cgctcggcca 6180 gtgctagttg agcgcaacca gggcgacacc accatctccc tctgctgcat ttgtgacaac 6240 cccggattgc atgaaagctt ttgttgtctt ctttgtactg agtataataa aagctgaaat 6300 tagagactac tccggactct cttgtcgtct gaacaacacc aaccagaccc ttcacttcag 6360 cgggaaccag actactcttc actgtaaggc ttataactat aagtatctta cttggatata 6420 caaaggaaca ccgtttgctg tggtaaacag gtgctccaac gacggtgttc tcctcacctt 6480 cctaggcaac ttctccaact ttaccttttc tgttcgcaga aacaagctta ccctccttca 6540 gccctacttt cctgggatct atacctgcct cagtggacct tgcaaccaca cttttcacct 6600 gattgaaaac tctaccctta ccttcccagc gccaatccct actaacagct cggagtccaa 6660 ctcttccatt accgctgata ctaacactcc taaaaccgga ggtgagctcc gcagccttcc 6720 cccggctgca gataaccctt gggtggtagc gggatttgta gcgctaggaa tagttgcggg 6780 tgggctcgcg ttcgtcctct gctacctata ccttacctgc tgctcatatt tagtagtact 6840 gtgctgttgg tttagaaaat gggggcgcta ctaatcacac ttgctttact ttcgcttttg 6900 ggtctgagct cggctaatag cgagaaacca agctgtctag aaacaaactc tccagactgt 6960 gtggttcctc atgggctctc agacccagct gatgatccat gcttaacttt tgacccagaa 7020 aaaaactgct cggtgactat gcagccctat gcttacatgt gcacatctgt tataaagtgc 7080 ggatggggct gtaaaccgat tgaaattacc cacaaaggca aaacctggaa taatagtttg 7140 tttaacacat ggcagcctgg agacgagcag tggtatacgg ccggccactg gtggagatga 7200 ctgaccccat ggaaaactcc tctgccaacg acctggacat ggacggccgt tcatctgagc 7260 agcgactggt ccagatgcgc attcgccaga agcaggaacg cgccgccaga gagctcaagg 7320 atgccattga aattcacctg tgcaagaagg gcatcttttg cttggttaag caagcaaaga 7380 tttcttatga aatcactgac aacgaccacc gcctgtatta tgagctcggt ccacagcggc 7440 agaaattcac ctgcatggtt ggagtcaacc ccatagtcat cactcagcag gctgcagaaa 7500 ttaaagggtg catccactgt tcctgtgatt cccaagaatg cgtccacacc atagtcaaga 7560 ccctctgcgg ccttcgagat cttcttccaa tgaactaacc ccttccccca acccaataaa 7620 acattggttt taatcataat aaaaaatcac ttactttaaa tctgaaacag tgtctccgtc 7680 caagttttct tgtagcacca cttcactccc ctcttcccag ctctggtact gcaagccccg 7740 gtgggctgca aactttctcc acaccttaaa agggatgtca aattcctctt gtccaacaat 7800 cttcattgtc tcttcctaga tgtccacaaa gcgcgcgcgg gtggaagatg actttgaccc 7860 tgtctaccca tacgatgctg agctggcacc gtctgtaccc ttcatcgccc ctcccttcgt 7920 ttcgtcagac ggatttcaag aaaaacccct gggagttctg tccctaagac tagccaaccc 7980 agtcactact aaaaatgggg aactcacact taaactggga gatggggtgg gcatagactc 8040 agatggaaac ctcacagcac agacagttac taaagcaaca tcccccctta ctgtttccaa 8100 taacgcaatt gcacttaaca tggacaaacc tttttacagt agcaatggaa aactatcctt 8160 acaagttaca tcaccattaa agatagtcga ctctttaaat acattggcta ttggctatgg 8220 gcaaggctta ggactaaaca atagtgctct tgctgtgcaa ttagcatctc cccttacatt 8280 tgacagcaac agcaaaatta aaataaattt gggaagcggg ccattaaaaa ttaatgcgaa 8340 taaactgtca attaactgcc taaggggtgt atatgtaaca actgacggaa cttccattga 8400 aacaaatata agctgggcaa aaggaatgag gtttgaaggt aatgccatgg ctgtaaacgt 8460 tgacagcacc aaaggtctac aatttggcac taccagcaca gaatcaggag tcactaacgc 8520 tttccctatc cagttaaaga ttggatctgg tcttagtttt gacagcacag gagcacttgt 8580 agcttgggat aaggataatg acaagcttac actgtggaca accgctgacc catcacctaa 8640 ttgtaccata tatacagaca aggatgctaa acttacactt tgtcttacaa aatgtggcag 8700 tcaaatacta ggcagtgttt cagtactggc tgttaaagct ggaaccctac agccaatcag 8760 tgaaaaaata ggtactgctt tggtttcact aaaatttaat aacaacggtg tattgttaag 8820 caactccaca ttaagtaatg aatactggaa ctacaggaag ggagatgtca caccagccga 8880 agcctatact aatgctgtgg gttttatgcc aaacatcaag gcatatccta aaaacacaaa 8940 ctctgcctca aaaagccaca ttgtaggaca agtgtacctt aatggagatg aaactaaacc 9000 aatgcattta atcattacat ttaatgaaac cagtgatgaa acatgcacat attccataac 9060 gttccaatgg aaatggaaca ttggaacata caccagcgac acccttgcaa caagctcctt 9120 taccttttct tacattgccc aagaataaaa actgcagaca acaataaagt ttaaatgttt 9180 tatttaaaca gtttcacaga accctagtat tcaacctgcc acctccctcc caacacacag 9240 agtacacagt cctttctccc cggctggcct taaaaagcat catatcatgg gtaacagaca 9300 tattcttagg tgttatattc cacacggttt cctgtcgagc caaacgctca tcagtgatat 9360 taataaactc cccgggcagc tcacttaagt tcatgtcgct gtccagctgc tgagccacag 9420 gctgctgtcc aacttgcggt tgcttaacgg gcggcgaagg agaagtccac gcctacatgg 9480 gggtagagtc ataatcgtgc atcaggatag ggcggtggtg ctgcagcagc gcgcgaataa 9540 actgctgccg ccgccgctcc gtcctgcagg aatacaacat ggcagtggtc tcctcagcga 9600 tgattcgcac cgcccgcagc ataaggcgcc ttgtcctccg ggcacagcag cgcaccctga 9660 tctcacttaa atcagcacag taactgcagc acagcaccac aatattgttc aaaatcccac 9720 agtgcaaggc gctgtatcca aagctcatgg cggggaccac agaacccacg tggccatcat 9780 accacaagcg caggtagatt aagtggcgac ccctcataaa cacgctggac ataaacatta 9840 cctcttttgg catgttgtaa ttcaccacct cccggtacca tataaacctc tgattaaaca 9900 tggcgccatc caccaccatc ctaaaccagc tggccaaaac ctgcccgccg gctatacact 9960 gcagggaacc gggactggaa caatgacagt ggagagccca ggactcgtaa ccatggatca 10020 tcatgctcgt catgatatca atgttggcac aacacaggca cacgtgcata cacttcctca 10080 ggattacaag ctcctcccgc gttagaacca tatcccaggg aacaacccat tcctgaatca 10140 gcgtaaatcc cacactgcag ggaagacctc gcacgtaact cacgttgtgc attgtcaaag 10200 tgttacattc gggcagcagc ggatgatcct ccagtatggt agcgcgggtt tctgtctcaa 10260 aaggaggtag acgatcccta ctgtacggag tgcgccgaga caaccgagat cgtgttggtc 10320 gtagtgtcat gccaaatgga acgccggacg tagtcattct cgtactttga gtggcaaaac 10380 cttgctctcg aacagcacac gtctcgtcgc ctcctgtccc ttctcttcgc cttttcagtg 10440 tgatagttgt aatacagcca ttcacgaagc tcagtcagaa gatcttcagc gtctgttgtc 10500 aaaaacaatc catccaatct gattgctttc aaaacatcac aaacagtcga ataagccaaa 10560 cccatccagg caatgcaatt attttggtta tccacaatgg gagggggcgg aagacatgga 10620 agaggcataa ttaatttttt aatccaatcg atcacgcagc acttcaaaat gaagatcgcg 10680 aaggtgacac ctttcacccc cactgtgttg atgaaaaata acagccaagt caaaattgat 10740 gcggttttca aggtgctcga ctgtagcatc aagcagagct tccacacgca cgtccacaaa 10800 taacagaata gcaaaagcgg gaggaggaag taaatcctca atcatcatag tacagtccat 10860 caccatccct aaataatttt catccttcca gccttggact atatttttaa actgctcttg 10920 taaatccaaa ccacacatgt ggaaaagttc ccaaagagct ccctcaacta ccattcttaa 10980 gcacaccttc atagtgacaa aatatcttgt tcctctgtca cctgcagcaa attacaaagt 11040 ccaatattag gatctatgcc cagagatcta agctcatccc tcaattccaa ctgtaaaaag 11100 gcttccagat ctgccctaac ttgttcagcc agtgggctcc ctggaataag cgtgggagaa 11160 gccaaactgc aaaacagacg catgccgcca taattaccac cagaaaacac tacgttacag 11220 tatgcatgct gattcattcc agtaatttca tccagtgtat tggatacaaa aaaaggcaag 11280 cactctctca ctaattgtat tatggagaca ttatcacaca ggtaacaatt taaaggttgt 11340 ggaacaataa tgcagtaagt aaccacggtg cgctccaaca tggttagtaa tttttagttc 11400 tgaaaaacaa aacatacaaa aaattatatc atactcattt ggcgaactgg tggaaaaatg 11460 accctatcta gcacaaggca agccactgga tcaccaatgc gcccctcata aaacctgtca 11520 tcatgattaa aaagcaacac cgaaagctct tccctatgtc ctgcatgaat gattctagct 11580 gaggaatata agccagcgca attagtatct gttaaagaaa aaaaacggcc aacatagcct 11640 ctaggaatta gcacacttaa tcttaaagac attactgcca tcccccttgg atttaaggta 11700 aaatttacag gagcatagaa aatatactga tttccctcct gcacaggcag catagcacca 11760 ggtccctcta aaaacacaca caaagcttct gcagccatag cttaccgcgc aaaccaggca 11820 cagcagtgag ctaaaaggac aaagctctaa ctcactagcc aacctggcgc acaatatata 11880 gttagtcctt acactgacgt aaccgaccaa agtctaaaaa ccccgccaaa aatacacaca 11940 cgcccaaaaa acgccccgtg agtcaaaaaa cagtttcact tcctcgttac acccaaaacg 12000 tcgtcacttc cggattccca cggttcgtca cttccggagc tccttgctta attaaccccg 12060 cccaaaacgt catcgtccgc gtcacgccgc cccgccccgc gaccgttgac cccgggccaa 12120 tcaccgcaca tcccgcaaaa ttcaaactcg tctaatttgc atattggcac actgcccata 12180 taaggtatat tattgatgat gatttaaatc atatgcggtg tgaaataccg cacagatgcg 12240 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 12300 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 12360 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 12420 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 12480 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 12540 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 12600 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 12660 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 12720 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 12780 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 12840 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 12900 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 12960 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 13020 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 13080 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 13140 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 13200 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 13260 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 13320 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 13380 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 13440 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 13500 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 13560 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 13620 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 13680 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 13740 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 13800 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 13860 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 13920 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 13980 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 14040 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 14100 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 14160 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 14220 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattga 14280 tttaaat 14287 <210> 19 <211> 638 <212> DNA <213> Artificial Sequence <220> <223> Ad5 pIX promoter insertion <400> 19 cgcgatcgcg gtactgaaat gtgtgggcgt ggcttaaggg tgggaaagaa tatataaggt 60 gggggtctta tgtagttttg tatctgtttt gcagcagccg ccgccgccat gagtgggagc 120 gcttcctttg aagggggcgt ctttagccct tatctgacgg ggcgtctgcc tcattgggct 180 ggagtgcgtc agaatgtgat ggggtctaca gtggatggaa gacctgttca gcctgctaat 240 tcttctactc tgacttatgc tactatgact tcctcgcctt tggatgcagc tgcagctgct 300 gccgcttctg ctgccgccaa cactgttcgg gggatggcct tggagatggg gtattatgga 360 actgtagtgg ccaacaccac taccccaaat aaccccacag ccttgaatga ggacaagctg 420 ctagttctca tgtcccagct ggagtctttg acccaacgcc tgggcgatct agctcagcag 480 gtgtcccagc tgaaggagca gactcaagct gcaattacca ctgcgagggg aaattaaaaa 540 aattcaaaga atcaataaat aaaccgagac tttgttgatt ttaaagtgtg tcattcttta 600 tttaattttt cgcgcgcgat atgccctgga ccaccggt 638 <210> 20 <211> 35711 <212> DNA <213> Artificial Sequence <220> <223> pBLY6.FLuc <400> 20 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cggcgatcgc tcaatattgg ccattagcca 480 tattattcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt 540 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac 600 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 660 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 720 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 780 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 840 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 900 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 960 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 1020 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 1080 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 1140 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 1200 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 1260 gcctccgcgg ccgggaacgg tgcattggaa gcttggcatt ccggtactgt tggtaaagcc 1320 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 1380 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 1440 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 1500 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 1560 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 1620 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 1680 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 1740 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 1800 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 1860 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 1920 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 1980 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 2040 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 2100 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 2160 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 2220 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 2280 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 2340 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 2400 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 2460 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 2520 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 2580 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 2640 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 2700 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 2760 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 2820 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 2880 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 2940 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agacgagatc cgaacttgtt 3000 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 3060 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 3120 ctgcgatcgc gatggcctgt gtttgagcat aatgtactga ccaggtgtaa cgttcatctg 3180 ggtggtcgta gaggaatgtt catgccatac caatgcaatt ttaatcatgt gaggatcttg 3240 atggagccgc aagcgttttc cagagtcagc ttgactggaa tctttgacat gtgtgtggaa 3300 gcatggaaga tcttaagata tgatgatacc aaatccagat gccgcgcatg cgagtgcggg 3360 ggcaggcatg ccaggttcca acctgtatgt gtggaggtga ccgaggagct gagaccagat 3420 catttggtgc tgacctgcac tggtgcggag ttcggttcca gtggtgaaga aactgattaa 3480 agtgagtagt gggatgttat aaaagtgacc ataaggtgat gtgagatgga caaatttggt 3540 aatttttatg tatttttgtc ttgcagccat gagtgggagc gcttcctttg aagggggcgt 3600 ctttagccct tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat 3660 ggggtctaca gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc 3720 tactatgact tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa 3780 cactgttcgg gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac 3840 taccccaaat aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct 3900 ggagtctttg acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca 3960 gactcaagct gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat 4020 aaaccgagac tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat 4080 atgccctgga ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat 4140 agaggtggga ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc 4200 tccactgcag agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc 4260 gttgggcatg atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct 4320 tggtgtaagt gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt 4380 gcattttgga ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca 4440 tgttatgcaa gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt 4500 tggaggggaa agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc 4560 actcatccat aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg 4620 gatctgacac atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact 4680 tggggcggag ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc 4740 cttcacatat ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg 4800 gagcgatgaa gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc 4860 taagcagctg tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca 4920 gatggtagtt tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca 4980 tcatttccct cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca 5040 gggaaaggag ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg 5100 gcattttgga gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt 5160 ctatggcatc tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta 5220 tggaaccagg cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt 5280 ccgagtcagg gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag 5340 ggtgcgcttc aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc 5400 ggccaggtag caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc 5460 gcgcagctta cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata 5520 gagttttggg gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca 5580 gacggtttcg cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt 5640 tccgccatgt tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg 5700 ggtgacaaag aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg 5760 cgtgccgcgg tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca 5820 ggccagcaca aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac 5880 tttttccaag gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt 5940 gtaagtgtat gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct 6000 ctgctcgtcc tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta 6060 ttccctctcg aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga 6120 ggatttgata ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc 6180 agaaaagaca atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga 6240 aagcagtttg gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc 6300 ggctatgttg agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt 6360 taattcatct gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac 6420 actggttgcc acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg 6480 agaacagaaa gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt 6540 gaaaatccca ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga 6600 catttgccat tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg 6660 catgggatgg gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc 6720 ttccagaatc cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata 6780 atcatagagt tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt 6840 ttctgctctg tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct 6900 ttggaagatg ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata 6960 ggagtcttgc agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc 7020 gagggtctct ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt 7080 gagaaggtat tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga 7140 acggtaagaa cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc 7200 cacggggagt gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt 7260 gtccctgacc atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg 7320 ctcccacagt tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac 7380 atcgttgaat aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg 7440 aggcacttct gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt 7500 gatgttgtga cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag 7560 ttttttaagc tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc 7620 ccagtcaggc aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc 7680 tgtttgtaag cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt 7740 aacacagtaa aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag 7800 gtcgtgggcg aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg 7860 gacaagctgc ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa 7920 gagcctttct gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt 7980 ggaggaatgg ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg 8040 cttgtgcttg tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat 8100 gagctgtacc tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg 8160 catctcgtgt tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt 8220 catgctgacg agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc 8280 gaggacgaga gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc 8340 agtagggaga gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag 8400 atgatatttg atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg 8460 cccctggggt gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg 8520 catggtaagg tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt 8580 tctggacctg gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg 8640 tactgcgccc tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga 8700 cgcctctggg tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa 8760 tcaatttcgg tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg 8820 tcttggtagg cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg 8880 cccgctcgtt ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat 8940 gcattcatgc ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg 9000 gcgcgcatga ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg 9060 cagagacgct ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac 9120 atgatccagc gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg 9180 gcttcgtaaa agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac 9240 tcctcctcca gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct 9300 cccgggatct cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact 9360 tcctcctcag gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc 9420 agacgatcga tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg 9480 gcgcgcccgt cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg 9540 tgactgggag ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc 9600 cctgtaggga ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc 9660 tgaacgaagg cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg 9720 cgggctgctg agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt 9780 ctgagacggc ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc 9840 aggcggtcgg ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct 9900 tgcatgagtc gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt 9960 ccgtaccctc tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg 10020 gcttgctgca cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag 10080 gcccccgtgt tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt 10140 cctggtcgta cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa 10200 tcgttgcagg ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg 10260 tagagaggcc atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg 10320 tggtatccgt agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc 10380 ggaaactctc gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc 10440 acggtctggc ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa 10500 gcgttgagcg gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac 10560 cctggttcga gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta 10620 ttggcaatcc cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg 10680 tttttcaggg catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt 10740 ggctcgcgcc cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt 10800 cgagcctgca gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg 10860 tttctaagac cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt 10920 tttgtttttg ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc 10980 agcagcaggc tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg 11040 ctgtaagcgg ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg 11100 agggattggc aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa 11160 aggacgttcg cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg 11220 agcccgagga gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg 11280 atcgaaaacg ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc 11340 ccgctcgcgc tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg 11400 aggaaagcaa cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg 11460 aggtgaccct gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca 11520 ctagcaaacc cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg 11580 cattcagaga ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg 11640 tgaatatcct gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg 11700 cggcgatcaa ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa 11760 ccccttacgt gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc 11820 tgaaagtgct aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg 11880 cggtgagcgc cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac 11940 gagccctgac gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc 12000 attggctgcc tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag 12060 tagtagacga ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt 12120 tttgctagat ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt 12180 ccggcattaa ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga 12240 cccgcaaccc cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg 12300 aggccgtggt gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg 12360 ccctggtgga gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc 12420 tcgagcgcgt ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga 12480 ccgacgtgcg cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt 12540 ccatggtagc gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg 12600 aagactatac caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg 12660 aggtgtacca gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag 12720 tgaacttaag ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg 12780 gcgacagggc gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg 12840 tgtccccctt cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa 12900 acttgtatcg cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca 12960 ctaatgtgag tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact 13020 ttctgctgac caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg 13080 aacggatctt gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga 13140 cccctagcgc cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca 13200 acagaccatt cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg 13260 attacttcac caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg 13320 gcgagtatga catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg 13380 cgttctcgcc taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct 13440 cctccgtgct gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct 13500 ttcccagtct gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc 13560 gccctcgctt gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg 13620 aaaagaactt ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga 13680 cctatgccca ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca 13740 gacgccagcg ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg 13800 acagcagcgt gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc 13860 gtccccgtat tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat 13920 ggcgaccagc gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg 13980 ctaggcggat cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag 14040 caggtggcgg cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac 14100 ctggcaccta ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac 14160 accacccggt tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag 14220 aacgaccaca gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag 14280 gccagcaccc agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc 14340 atcatgcaca ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct 14400 agggtgatgg tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt 14460 agtcaggaca tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct 14520 gccaccatga ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt 14580 agacagaatg gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg 14640 cttggttggg acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc 14700 catcctgaca tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt 14760 aatctgctag gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag 14820 gatctggagg gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag 14880 gaagataata caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa 14940 gctgtggtag atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga 15000 gatacatttg ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt 15060 ctgtccaccg atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat 15120 agtaaggaca ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg 15180 tacctggcat acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc 15240 acctcggatg tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa 15300 gatccagtca cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag 15360 ctgctcccag tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc 15420 cgccagtaca cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc 15480 cgcccgcccg cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac 15540 gggaccctgc cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc 15600 agacgccgca cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt 15660 tcaagccgca ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg 15720 gggcctgcgc acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc 15780 tgtgcgcgtg cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac 15840 taggaccacc gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc 15900 cgcagccgca cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg 15960 cgcctatgct cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc 16020 agccatgcga gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat 16080 gcgtagagcg gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc 16140 ggctacggcg gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta 16200 ttgggtgcgc gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac 16260 ttagaagata ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa 16320 ttcaaggaag agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat 16380 gaaaaaaagc cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat 16440 gatgggctgg tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg 16500 cgcaaagtgt ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc 16560 tccagcaccg tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag 16620 caggcggctg atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag 16680 gaagaggcat tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg 16740 accctgcaac aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat 16800 ctgtatccca ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag 16860 aaaatgaaag tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg 16920 gcgcccggtt tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt 16980 caaaccgaac ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg 17040 cccgcgcctg ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc 17100 aacctgataa tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc 17160 ggcactcgct actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc 17220 cgtcgccgcc gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac 17280 cgtgatgggc gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc 17340 atttaacaac tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt 17400 gctggctacc gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg 17460 cgtcgccacc ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct 17520 ttgattccca tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg 17580 caggcctcgc agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga 17640 ctctgacgct cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt 17700 catccctggc tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca 17760 acagccaact gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt 17820 ttgggtccac tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc 17880 tgagacaaaa gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg 17940 gaatcaatgg ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc 18000 tggacccggc tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt 18060 cattggaaaa gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca 18120 aggtggatga gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc 18180 ccattatgcc tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg 18240 ctacattgga cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca 18300 ccgccgtgca acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact 18360 ggcagagcac tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct 18420 gctattgatt aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg 18480 ccgcccgccg tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag 18540 atggccaccc catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct 18600 tcggagtacc tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat 18660 ctggggaaca agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt 18720 agccagcgcc tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac 18780 aaagtcagat acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac 18840 tttgacatca ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc 18900 tacaactccc tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac 18960 aatggtcaag gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac 19020 attgacaaaa atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt 19080 tatgcagaca aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc 19140 gaaaactatt atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt 19200 tccttcgcta gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag 19260 ggtcaacagt ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc 19320 ggaggcaatg gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta 19380 aatctggaaa ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc 19440 gaggccaatt taactcagca agccatggct aacagaccca actatattgg gtttagagat 19500 aactttattg gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa 19560 gcatcccagc taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag 19620 ctattacttg actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg 19680 gacagctatg atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca 19740 aactattgct tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca 19800 aaagaggata ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata 19860 tgcaagggca acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc 19920 ctttactcca acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg 19980 acactcccta ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc 20040 ctggtggatg cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc 20100 aaccctttca accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac 20160 ggccgatacg tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg 20220 ctgcttctcc caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc 20280 ctgcagagtt cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc 20340 atcaatctct atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc 20400 atgctgcgca atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg 20460 ctttacccca tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg 20520 gctgccttca ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga 20580 tcagggttcg atccctactt cgtctactca ggctccattc catacctgga tggaactttc 20640 taccttaacc acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca 20700 ggcaatgaca ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa 20760 gggtacaatg tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc 20820 cactacaaca ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac 20880 tccttcttca gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa 20940 gactacatgg cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc 21000 gcgcccacca tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc 21060 aagactgcag tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc 21120 atccccttct ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg 21180 ctttatgcca actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag 21240 tccacccttc tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac 21300 cgcggcgtca tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca 21360 taagcgcctt gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat 21420 ccaatgagca agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg 21480 gaacctttga caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg 21540 tcaacacggc agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc 21600 gctccaatac ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga 21660 tctatcaatt cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat 21720 gcatcactct ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc 21780 tcttctgctg catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca 21840 accccaccat gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag 21900 agcccaccct gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt 21960 actttcgttc taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata 22020 acatgtaaac caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg 22080 aggctctggg atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc 22140 agatggcagg gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac 22200 cagcctggga actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag 22260 agcgccaagc aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc 22320 gcgggagttg cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac 22380 gctcgccagc acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat 22440 tccaaagggg gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg 22500 gttgcaatca caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg 22560 gtaaacagcc ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc 22620 ggtgtagaac actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt 22680 cacacagcag cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg 22740 ggtgatcttg gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac 22800 atccatctca atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt 22860 gccttcataa tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt 22920 atgggggatc tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc 22980 cagggtcttt atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg 23040 ctggcagatg cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa 23100 ctcattatcc agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca 23160 ggcagaaaca atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg 23220 gtcatcactg ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg 23280 cgggtagctg aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg 23340 actgatgtct tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc 23400 tggaggactc tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac 23460 ttggatctgg ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt 23520 aggcagaggt ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc 23580 ccttccgcgt tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc 23640 cattgtgttc tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc 23700 atccaccacc acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc 23760 cgctaccacc accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc 23820 tatggaagat attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca 23880 tgatgaggat ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga 23940 ggaagaggca gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga 24000 cgtgctgctt aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg 24060 ctccgaggtg ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc 24120 gcctcgcaag ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa 24180 cttctatcca gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa 24240 ccaaaagatc cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt 24300 ggggcccggc gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga 24360 gggtctgggc aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg 24420 tgaacaccac agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa 24480 acgcagcatc gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat 24540 gagctcggtc atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa 24600 catgcaggac cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc 24660 tcgctggcta ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc 24720 agtggtcctg atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac 24780 cctgcgcaag cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc 24840 gtgcaagatc tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga 24900 aaaccggctg gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta 24960 tatccgcgac tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg 25020 gcaacagtgc ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct 25080 taaatctctg tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat 25140 cttccccgag cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag 25200 catgctacaa aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg 25260 ctgcgcactg ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg 25320 gagccactgc tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga 25380 agatgttagt ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca 25440 ccgctccctc gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga 25500 gttgcaaggt cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac 25560 tccggggcta tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta 25620 tgagatcagg ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt 25680 catcacccag ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt 25740 tttgctgaaa aagggacacg ggatctatct agacccccag accggtgagg agctgaatac 25800 acgcttccct caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg 25860 aggagctgga agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag 25920 aggaggacag cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag 25980 ccgccgccag accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta 26040 ccacctccgc tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg 26100 gtcgcttcca gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt 26160 cctggcgggg gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat 26220 cctttgccag acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc 26280 attactaccg tcacctccac agcccctact accagcagca agagacagca gaggaaacca 26340 gcggcaactc cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt 26400 gcaggcggac tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg 26460 atctttccca ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa 26520 gtaaaaaaca gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac 26580 caacttcagc gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact 26640 cttaaagagt agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc 26700 cacacctgtg cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc 26760 cccagatggg attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca 26820 gcgccggtcc cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac 26880 tcctagaaca gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc 26940 ccgccgccct ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg 27000 cccaggccga agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt 27060 gtcgtcaccg gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc 27120 tcaacgacga gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag 27180 ctggttgtgg gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt 27240 cctcgcagcc ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct 27300 cggtctactt caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga 27360 actacgacgc catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc 27420 tgagctagct cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga 27480 tctctgcgag ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg 27540 agttcggatt accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg 27600 gccagtgcta gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga 27660 caaccccgga ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg 27720 aaattagaga ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact 27780 tcagcgggaa ccagactact cttcactgta aggcttataa ctataagtat cttacttgga 27840 tatacaaagg aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca 27900 ccttcctagg caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc 27960 ttcagcccta ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc 28020 acctgattga aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt 28080 ccaactcttc cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc 28140 ttcccccggc tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg 28200 cgggtgggct cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag 28260 tactgtgctg ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct 28320 tttgggtctg agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga 28380 ctgtgtggtt cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc 28440 agaaaaaaac tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa 28500 gtgcggatgg ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag 28560 tttgtttaac acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag 28620 atgactgacc ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct 28680 gagcagcgac tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc 28740 aaggatgcca ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca 28800 aagatttctt atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag 28860 cggcagaaat tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca 28920 gaaattaaag ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc 28980 aagaccctct gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa 29040 taaaacattg gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc 29100 cgtccaagtt ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc 29160 cccggtgggc tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa 29220 caatcttcat tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg 29280 accctgtcta cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct 29340 tcgtttcgtc agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca 29400 acccagtcac tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag 29460 actcagatgg aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt 29520 ccaataacgc aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat 29580 ccttacaagt tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct 29640 atgggcaagg cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta 29700 catttgacag caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg 29760 cgaataaact gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca 29820 ttgaaacaaa tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa 29880 acgttgacag caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta 29940 acgctttccc tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac 30000 ttgtagcttg ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac 30060 ctaattgtac catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg 30120 gcagtcaaat actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa 30180 tcagtgaaaa aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt 30240 taagcaactc cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag 30300 ccgaagccta tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca 30360 caaactctgc ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta 30420 aaccaatgca tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca 30480 taacgttcca atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct 30540 cctttacctt ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat 30600 gttttattta aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac 30660 acagagtaca cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca 30720 gacatattct taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg 30780 atattaataa actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc 30840 acaggctgct gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac 30900 atgggggtag agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga 30960 ataaactgct gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca 31020 gcgatgattc gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc 31080 ctgatctcac ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc 31140 ccacagtgca aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca 31200 tcataccaca agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac 31260 attacctctt ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta 31320 aacatggcgc catccaccac catcctaaac cagctggcca aaacctgccc gccggctata 31380 cactgcaggg aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg 31440 atcatcatgc tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc 31500 ctcaggatta caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga 31560 atcagcgtaa atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc 31620 aaagtgttac attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc 31680 tcaaaaggag gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt 31740 ggtcgtagtg tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca 31800 aaaccttgct ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc 31860 agtgtgatag ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt 31920 tgtcaaaaac aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc 31980 caaacccatc caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca 32040 tggaagaggc ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat 32100 cgcgaaggtg acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat 32160 tgatgcggtt ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca 32220 caaataacag aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt 32280 ccatcaccat ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct 32340 cttgtaaatc caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc 32400 ttaagcacac cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca 32460 aagtccaata ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa 32520 aaaggcttcc agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg 32580 agaagccaaa ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt 32640 acagtatgca tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg 32700 caagcactct ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg 32760 ttgtggaaca ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta 32820 gttctgaaaa acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa 32880 aatgacccta tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct 32940 gtcatcatga ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct 33000 agctgaggaa tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata 33060 gcctctagga attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa 33120 ggtaaaattt acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc 33180 accaggtccc tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca 33240 ggcacagcag tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata 33300 tatagttagt ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca 33360 cacacgccca aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa 33420 aacgtcgtca cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac 33480 cccgcccaaa acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg 33540 ccaatcaccg cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc 33600 catataaggt atattattga tgatgattta aatcatatgc ggtgtgaaat accgcacaga 33660 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 33720 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 33780 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 33840 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 33900 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 33960 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 34020 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 34080 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 34140 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 34200 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 34260 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 34320 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 34380 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 34440 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 34500 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 34560 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 34620 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 34680 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 34740 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 34800 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 34860 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 34920 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 34980 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 35040 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 35100 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 35160 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 35220 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 35280 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 35340 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 35400 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 35460 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 35520 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 35580 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 35640 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa 35700 ttgatttaaa t 35711 <210> 21 <211> 36702 <212> DNA <213> Artificial Sequence <220> <223> pBLY6.RSVF-2A-GLuc <400> 21 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgct caatattggc cattagccat 480 attattcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta 540 tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca 600 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 660 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 720 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 780 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 840 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 900 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 960 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 1020 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 1080 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 1140 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat 1200 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag 1260 cctccgcggt tggccgtgct cttcctgacg ggtaggtgtc ccctaaccta gggagccaac 1320 catcgggggg ccttctccct aaatccccgt ggcccaccct cctgggcaga ggcagcaggt 1380 ttctcactgg ccccctctcc cccacctcca agcttggcct ttcggctcag atctcagccc 1440 acagctggcc tgatctgggt ctcccctccc accctcaggg agccaggctc ggcatttcgt 1500 cgacatggaa ctgctgatcc tgaaggccaa cgccatcacc accatcctga ccgccgtgac 1560 cttctgcttc gccagcggcc agaacatcac cgaggaattc taccagagca cctgtagcgc 1620 cgtgtccaag ggctacctga gcgccctgcg gaccggctgg tacaccagcg tgatcaccat 1680 cgagctgagc aacatcaaaa agaacaagtg caacggcacc gacgccaaaa tcaagctgat 1740 caagcaggaa ctggacaagt acaagaacgc cgtgaccgag ctgcagctgc tgatgcagag 1800 cacccccgcc accaacaacc gggccagacg ggagctgccc cggttcatga actacaccct 1860 gaacaacgcc aaaaagacca acgtgaccct gagcaagaag cggaagcggc ggttcctggg 1920 cttcctgctg ggcgtgggca gcgccattgc tagcggagtg gctgtgtcta aggtgctgca 1980 cctggaaggc gaagtgaaca agatcaagtc cgccctgctg agcaccaaca aggccgtggt 2040 gtccctgagc aacggcgtgt ccgtgctgac cagcaaggtg ctggatctga agaactacat 2100 cgacaagcag ctgctgccca tcgtgaacaa gcagagctgc agcatcagca acatcgagac 2160 agtgatcgag ttccagcaga agaacaaccg gctgctggaa atcacccgcg agttcagcgt 2220 gaacgccggc gtgaccaccc ccgtgtccac ctacatgctg accaacagcg agctgctgag 2280 cctgatcaac gacatgccca tcaccaacga ccagaaaaag ctgatgagca acaacgtgca 2340 gatcgtgcgg cagcagagct actccatcat gtccatcatc aaagaagagg tgctggccta 2400 cgtggtgcag ctgcccctgt acggcgtgat cgacaccccc tgctggaagc tgcacaccag 2460 ccccctgtgc accaccaaca ccaaagaggg cagcaacatc tgcctgaccc ggaccgaccg 2520 gggctggtac tgcgataatg ccggcagcgt gtcattcttt ccacaagccg agacatgcaa 2580 ggtgcagagc aaccgggtgt tctgcgacac catgaacagc ctgaccctgc ccagcgaggt 2640 gaacctgtgc aacgtggaca tcttcaaccc taagtacgac tgcaagatca tgacctccaa 2700 gaccgacgtg tccagctccg tgatcacctc cctgggcgcc atcgtgtcct gctacggcaa 2760 gaccaagtgc accgccagca acaagaaccg gggcatcatc aagaccttca gcaacggctg 2820 cgactacgtg tccaacaagg gcgtggacac cgtgtccgtg ggcaacaccc tgtactacgt 2880 gaacaaacag gaaggcaaga gcctgtacgt gaagggcgag cccatcatca acttctacga 2940 ccccctggtg ttccccagcg acgagttcga cgccagcatc agccaggtca acgagaagat 3000 caaccagagc ctggccttca tcagaaagag cgacgagctg ctgcacaatg tgaatgccgt 3060 gaagtccacc accaatatca tgatcaccac aatcatcatc gtgatcatcg tcatcctgct 3120 gtccctgatc gccgtgggcc tgctgctgta ctgcaaggcc cggtccaccc ctgtgaccct 3180 gtccaaggac cagctgagcg gcatcaacaa tatcgccttc tccaacggac gcgtgaccga 3240 gctgctttac cggatgaagc gggctgagac atattgcccg agacccctgt tggcaatcca 3300 tcctactgag gctcgccaca aacagaaaat cgtggccccc gtcaaacaga cactcaattt 3360 tgacttgttg aaacttgcag gagatgttga gtcaaacccc gggcctatgg gcgtcaaggt 3420 cctgttcgct ctgatttgta tcgctgtcgc tgaagctaag ccaaccgaga ataatgaaga 3480 ctttaatatc gtggccgtgg cttctaactt cgctaccaca gacctggatg cagacagggg 3540 aaagctgcca ggcaagaaac tgcccctgga ggtcctgaag gagatggaag caaatgcccg 3600 gaaagccggg tgcacaagag gatgcctgat ttgtctgagc cacatcaagt gcactcctaa 3660 gatgaagaag ttcatccccg gccggtgcca tacctacgag ggcgataagg aatccgccca 3720 gggaggaatc ggagaggcta tcgtggatat tcccgaaatc cctggcttca aagacctgga 3780 gcccatggaa cagtttattg cacaggtgga tctgtgcgtc gactgtacta ccggatgcct 3840 gaagggactg gcaaacgtcc agtgtagcga cctgctgaag aaatggctgc ctcagcgatg 3900 tgctacattt gccagcaaga ttcagggcca ggtggacaag attaagggag caggaggcga 3960 ctgataattc tagacgagat ccgaacttgt ttattgcagc ttataatggt tacaaataaa 4020 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4080 tgtccaaact catcaatgta tcttatcatg tctgcgatcg cgatggcctg tgtttgagca 4140 taatgtactg accaggtgta acgttcatct gggtggtcgt agaggaatgt tcatgccata 4200 ccaatgcaat tttaatcatg tgaggatctt gatggagccg caagcgtttt ccagagtcag 4260 cttgactgga atctttgaca tgtgtgtgga agcatggaag atcttaagat atgatgatac 4320 caaatccaga tgccgcgcat gcgagtgcgg gggcaggcat gccaggttcc aacctgtatg 4380 tgtggaggtg accgaggagc tgagaccaga tcatttggtg ctgacctgca ctggtgcgga 4440 gttcggttcc agtggtgaag aaactgatta aagtgagtag tgggatgtta taaaagtgac 4500 cataaggtga tgtgagatgg acaaatttgg taatttttat gtatttttgt cttgcagcca 4560 tgagtgggag cgcttccttt gaagggggcg tctttagccc ttatctgacg gggcgtctgc 4620 ctcattgggc tggagtgcgt cagaatgtga tggggtctac agtggatgga agacctgttc 4680 agcctgctaa ttcttctact ctgacttatg ctactatgac ttcctcgcct ttggatgcag 4740 ctgcagctgc tgccgcttct gctgccgcca acactgttcg ggggatggcc ttggagatgg 4800 ggtattatgg aactgtagtg gccaacacca ctaccccaaa taaccccaca gccttgaatg 4860 aggacaagct gctagttctc atgtcccagc tggagtcttt gacccaacgc ctgggcgatc 4920 tagctcagca ggtgtcccag ctgaaggagc agactcaagc tgcaattacc actgcgaggg 4980 gaaattaaaa aaattcaaag aatcaataaa taaaccgaga ctttgttgat tttaaagtgt 5040 gtcattcttt atttaatttt tcgcgcgcga tatgccctgg accaccggtc tctatcattg 5100 aggacacggt ggatcttttc tagaacccga tagaggtggg attggatgtt gaggtacatg 5160 ggcataagac catctttggg gtgtagatag ctccactgca gagcctcatg ctccggggtg 5220 gtgttgtata taacccagtc atagcatggg cgttgggcat gatgttgcac aatatcttta 5280 aggaggagac taatggccac tgggagaccc ttggtgtaag tgtttacaaa tctattaagc 5340 tgggacgggt gcatccgagg tgagataatg tgcattttgg attggatttt tagattggca 5400 atgtttcccc ctagatctct cctgggattc atgttatgca agaccactag aacagtgtat 5460 ccggtgcact tagggaattt gtcatgaagt ttggagggga aagcatgaaa aaatttagac 5520 acacccttgt gtcctcccaa gttctccatg cactcatcca taataatggc aatgggccca 5580 tgggcggcgg cacgggcgaa cacgttcctg ggatctgaca catcatagtt gtggtcttgg 5640 gtcaggtcat cataagccat tttaataaac ttggggcgga gggtgccaga ttgggggatg 5700 aatgttccct cgggccccgg aacatagttt ccttcacata tttgcatttc ccaggctttt 5760 agttcagagg gggggatcat gtccacctgt ggagcgatga agaagacggt ctcgggggcg 5820 ggggtgatta agtgggagga cagcaagttc ctaagcagct gtgacttgcc acacccagtg 5880 ggaccgtaga tgacccctat aacaggttgc agatggtagt ttagggaaag acagctgccg 5940 tcctctcgca ggaggggggc gacctcgttc atcatttccc tcacatgcat gttttcccgc 6000 acaagttccg ataggaggcg ctctccaccc agggaaagga gttcttgaag agatgagaaa 6060 tttttcaagg gttttaagcc atcagccatg ggcattttgg agagggtttg ttgcaagagt 6120 tcaaggcggt cccagagttc ggtgatgtgt tctatggcat ctcgatccag catacttcct 6180 cgtttctggg gttgggacgg ctgcgggagt atggaaccag gcgatgggcg tccagcgctg 6240 ccagtgtccg gtccttccac ggtcgcagcg tccgagtcag ggtcgtttcc gtcacggtga 6300 aggggtgcgc gcctggctgg gcgcttgcga gggtgcgctt caggctcatc ctgctcgtgg 6360 agaaccgctg ccgttctgcg ccctgtgcat cggccaggta gcaattaacc atgagttcgt 6420 agttgagcgc ctctgccgcg tggcctttgg cgcgcagctt acctttggaa gtcttctgac 6480 aggtgggaca gtagagacac ttgagagcat agagttttgg ggctagaaag accgattctg 6540 gggagtatgc atcggcccca caggaggcgc agacggtttc gcattccacc agccatgtaa 6600 gatcgggctc gttggggtca aaaacaagtt ttccgccatg ttttttgatg cgtttcttac 6660 ctttgctttc catgagttcg tgcccccgtt gggtgacaaa gaggctgtcc gtgtccccgt 6720 agactgactt tatgggcctg tcctcgagcg gcgtgccgcg gtcctcttcg tagaggaact 6780 cggaccactc tgagacgaaa gcacgtgtcc aggccagcac aaaggaggct atatgggagg 6840 ggtagcgatc gttgtcaacc aaggggtcta ctttttccaa ggtgtgtaaa cacatgtccc 6900 cttcttccac atccaggaag gtgattggct tgtaagtgta tgccacgtga cctggggtcc 6960 cagacggggg ggtataaaag ggggcgggtc tctgctcgtc ctcactgtct tccggatcgc 7020 tgtccaggag cgccagctgt tgaggtaggt attccctctc gaaggcgggc ataacctccg 7080 cactcaggtt gtcagtttct aggaacgagg aggatttgat attgacagtg cctgccgaga 7140 tgcctttcat gagactgtcg tccatttggt cagaaaagac aatctttttg ttatcaagtt 7200 tggtggcgaa ggatccatac agggcattgg aaagcagttt ggcaatggag cgcatggttt 7260 ggtttttttc tttgtctgcg cgctctttgg cggctatgtt gagttggaca tattcgcggg 7320 ccagacattt ccattgtgga aatatggtag ttaattcatc tgggacgatt ctgactttcc 7380 agcctctgtt atgcagggta atcagatcca cactggttgc cacttctcct ctaagtggtt 7440 cattagtcca gcatagtcgc cccccttttc gagaacagaa agggggtagg ggatctagca 7500 tgagttcgtc tggggggtct gcatctatgg tgaaaatccc aggaaggaga tcttcgtcaa 7560 aatagctgat ggtggcgggg tcatccagag acatttgcca ttctcgagca gccagagcgc 7620 gctcgtaggg gttaagggga gtcccccatg gcatgggatg ggtgagtgca gaagcataca 7680 tgccacagat gtcatagaca tagagcggct cttccagaat ccctatgtaa gtgggataac 7740 atcgcccccc tctgatgctg gctcgcacat aatcatagag ttcatgtgag ggcgctagaa 7800 gacccgagcc caggttggtg cggttgggtt tttctgctct gtagaggatc tggcgaaaga 7860 tggcatggga gtttgatgag atggtgggtc tttggaagat gttgaaatgg gcatgaggca 7920 gtcccacaga gtcccttatg aagtgagcat aggagtcttg cagtttggcc accagctcgg 7980 cggtgaccag cacatccaaa gcacagtagt cgagggtctc tttgatgatg tcatagttag 8040 gttccccttt cttttcccac agctcgcggt tgagaaggta ttcttcgcga tccttccagt 8100 actcttcgag ggggaacccg tccttgtctg aacggtaaga acccagcatg taaaattgat 8160 tgacagcttt gtaggcacaa caccccttct ccacggggag tgagtatgct tgcgcggctt 8220 tgcgcagaga ggtgtgagta agggcgaaag tgtccctgac catgactttg aggaactgat 8280 gcttaaagtc tatgtcatcg caggccccct gctcccacag ttggaagtcc actcgctttt 8340 tgtaggcggg attgggcaaa gcgaaagtaa catcgttgaa taggatcttt ccagccctgg 8400 gcatgaagtt gcgagtaatg cgaaaaggct gaggcacttc tgccctgttg ttgataactt 8460 gggcagccaa gacgatctcg tcaaagccgt tgatgttgtg acccacaatg taaagttcta 8520 cgaagcgtgg gcgtcccttg atgtggggca gttttttaag ctcttcgtag gtcaagtcgt 8580 cagggtcagc gattccatat tgctccaaag cccagtcagg caggtgagga ttagcatgaa 8640 ggaaagaggt ccaaagatcc acggccagag ctgtttgtaa gcggtctctg tactgacgga 8700 aatgtcggcc taccgccatt ttttcaggag taacacagta aaaggtgcgc gggtcctttt 8760 cccagcgatc ccattgaagt tgcaaggcta ggtcgtgggc gaggttgacg agctgttcgt 8820 cccccgaaag tttcatgacc agcatgaaag ggacaagctg cttgccaaag gaccccatcc 8880 aggtgtaggt ttccacatcg taggtgagga agagcctttc tgtgcgagga tgagaaccga 8940 tcgggaagaa ctggatttcc tgccaccagt tggaggaatg gctgttgata tgatggaagt 9000 agaactccct acggcgcgcc gagcattcgt gcttgtgctt gtacagacgg ccacagtact 9060 cgcagcgctg cacgggatgc acctgatgaa tgagctgtac ctggcttcct ttgacaagaa 9120 atttcagtgg gaagttgagg cgtggcgtct gcatctcgtg ttgtattacg tcctggctat 9180 tggtctggcc atcttctgtc tcgatggtgg tcatgctgac gagcccgcgc gggaggcagg 9240 tccagacctc cgcgcggacg ggtctgagag cgaggacgag agcgcgcagg ccggaactgt 9300 ccagggtcct gagacgctgc ggagtcaggt cagtagggag agtacatagg tttacttgca 9360 taagtttttc cagggcatgt gggaggtcaa gatgatattt gatttctact ggcgagttgg 9420 tggagacatc gatggcttgc agggtcccgt gcccctgggg tgctaccacc gtcccttttt 9480 ttttcttgat cgggggcggt gttgcttctt gcatggtaag gtcgtcttct agaagcggcg 9540 gcgaggtcgc gcgccgggtg gcagtggcgg ttctggacct ggaggtagag gcggtagagg 9600 tacgtcggcg ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac ttgcgtgagc 9660 gacgacgcgg cggttgacgt cctggatctg acgcctctgg gtgaatgcta ccggacccgt 9720 gagcttgaac ctgaaagaga gttcaacaga atcaatttcg gtatcgttga cggctgcctg 9780 ccgcaggatt tcttgtacgt cgcccgagtt gtcttggtag gcgatctcgg ccatgaactg 9840 ctcgatctct tcttcttgga gatctccgcg gcccgctcgt tctacggtgg cagcaaggtc 9900 gttggagatg cgccccatga gctgtgagaa tgcattcatg cccgcctcgt tccagacgcg 9960 actgtagacc acggctccct cgggatctct ggcgcgcatg accacttggg cgaggtttag 10020 ttccacgtgt ctggtgaaga ccgcatagtt gcagagacgc tggaagaggt agttgagcgt 10080 ggtggcgatg tgctcggtga caaagaaata catgatccag cgacgaagcg gcatctcgct 10140 gatatcgccc agggcttcca accgttccat ggcttcgtaa aagtccacgg cgaagttgaa 10200 aaactgggag ttgcgagcgg acacggtcaa ctcctcctcc agaagacgga tgagctcggc 10260 gatggtggcg cgcacttcgc gctcaaaggc tcccgggatc tcttcctcct cttcttcttc 10320 caactcttcc tccactaaca tctcttctac ttcctcctca ggcggcgggg gtggaggagg 10380 gggcgcgcgg cgacgccggc gacgcacggg cagacgatcg atgaagcgtt cgatcacttc 10440 tccgcggcgg cgacgcatgg tctcggtgac ggcgcgcccg tcctccctgg gtcgcagagt 10500 gaagacgccg ccgcgcagct ccctgaaatg gtgactggga gggtccccgt ttggtaggga 10560 cagggcactg atgatgcatc ttattaattg ccctgtaggg actccgcgca aggacctgag 10620 cgtctcgaga tccacgggat ctgaaaatct ctgaacgaag gcttcgagcc agtcgcagtc 10680 gcaaggtagg ctgagcactg tttcttcggg gcgggctgct gagctagagg gttgtacgat 10740 gctgctggtg atgaagttaa aataggcagt tctgagacgg cggatggtgg cgaggagcac 10800 caggtctttg ggtccggctt gctggatgcg caggcggtcg gccattcccc atgcattatc 10860 ttggcacctg gccagatctt tatagtagtc ttgcatgagt cgctccacgg gcacttcttc 10920 ttcgcccgct ctgccgtgca tgcgtgtgag tccgtaccct ctctgtggtt ggacgagcgc 10980 caggtcggca acgacccttt cggctagaat ggcttgctgc acctgggtga gggtgttctg 11040 gaaatcatca aagtccacaa agcggtggta ggcccccgtg ttgatggtgt aggagcagtt 11100 ggacatgacc gaccagttga ctgtctggtg tcctggtcgt acgagttccg tgtacctgag 11160 ccgcgagtat gcgcgggagt cgaagatgta atcgttgcag gttcgcacca ggtactggta 11220 gccgatgagg aagtgaggcg gcggctggcg gtagagaggc catcgttcgg tggcgggcgc 11280 gccgggcgct aggtcttcta gcatgagacg gtggtatccg tagacgtacc tggacatcca 11340 ggtaataccg gcggcggtgg tggaggcgcg cggaaactct cgcacgcggt tccagatgtt 11400 gcgcagcggc atgaagtagt tcatggtggg cacggtctgg cccgtgaggc gcgcgcagtc 11460 attgatgctc tagatacggg caaaaacgaa agcgttgagc ggttcccttc cgtggcctgg 11520 aggaacgcga acgggttagg tcgcagcgta ccctggttcg agactaaaga aagcgagcaa 11580 ctcgaaccgg cagagtcgcg gctaacgggt attggcaatc ccgtctcgac ccaagccagc 11640 aaatccagga tacggatggg ggcccctttt gtttttcagg gcatgagtca ccggttaagg 11700 tttacaacgg ctgtttcatg cctttagaag tggctcgcgc ccgtagtctg gagaatcaat 11760 cgccagggtt gcgttgcggc gtgccccggt tcgagcctgc agcttgagtc ggccggtgac 11820 cgcggcaaac gagggcgtgg cggccccgtc gtttctaaga ccttgctagc cgacctctcc 11880 agtttacggg aacgagcccc cttttatttt ttttgttttt gccagatgca tcccgtactg 11940 cggcagatgc gcccacagcc cccacagcag cagcagcagg ctggcctacc ttctctacct 12000 cagccgctac ctgcaactac cgcggtggcc gctgtaagcg gggccggaca gcaggcggct 12060 cctcaatatg aattggactt ggaagagggc gagggattgg caagattggg ggcgccctcg 12120 cccgagcgcc acccgcgggt gcagatgaaa aaggacgttc gcgaatctta cgtgcccaag 12180 cagaatctgt tcagagacag aagcggcgag gagcccgagg agatgcgcgc gtcccgtttt 12240 aacgcgggtc gcgagctgcg acaaggactg gatcgaaaac gggtgttgag ggatgatgat 12300 tttgaggtgg atgaaatgac agggatcagc cccgctcgcg ctcacgtggc tgcagctaat 12360 ctggtgacag cttatgagca gaccgtgaag gaggaaagca acttccagaa atcattcaat 12420 aaccacgtgc gcaccctgat cgcacgcgag gaggtgaccc tgggcctgat gcacctgtgg 12480 gatctgctgg aagccatagt gcagaacccc actagcaaac ccctgactgc tcaactgttt 12540 ctggtggtgc agcacagcag ggataatgag gcattcagag aggcgctgct gaatatcact 12600 gaacctgagg ggagatggct gctggatctg gtgaatatcc tgcagagcat tgtagtgcag 12660 gaacgcagct tgcctttgtc cgagaaggtg gcggcgatca attactctgt gctgagtctg 12720 ggcaaatact atgccaggaa gatctacaaa accccttacg tgcccataga caaggaagtg 12780 aaaatagatg ggttttacat gcgcatgacc ctgaaagtgc taaccctgag cgatgacttg 12840 ggagtgtacc gcaacgacag gatgcaccgc gcggtgagcg ccagcaggag gcgcgagctg 12900 agcgacaaag aattaatgca cagcttgcaa cgagccctga cgggagccgg gacggagggg 12960 gagaactact ttgacatggg tgcagacttg cattggctgc ctagtcgcag ggcattggaa 13020 gcggcaggcg atgggcccta tgtagaggaa gtagtagacg aggacgatga ggagggcgag 13080 tacctggaag actgatggcg cgacccgtat ttttgctaga tggaacaggc gccggaccct 13140 gcgatgcggg cggcgctgca gagccagccg tccggcatta attcctcgga cgattggacc 13200 caggccatgc aacgcatcat ggcgctgacg acccgcaacc ccgaagcctt tagacagcag 13260 cctcaggcca accgcctttc ggccatcctg gaggccgtgg tgccctctcg ctccaacccc 13320 acccacgaga aggttctggc catcgtgaat gccctggtgg agaacaaggc catccgctcc 13380 gatgaagccg ggctggtata caacgccttg ctcgagcgcg tggctcgcta caacagcagc 13440 aatgtccaga ctaacctgga caggatggtg accgacgtgc gcgaggccgt gtcccagcgc 13500 gaacggttcc atcgcgagtc taacctgggt tccatggtag cgctgaacgc tttcctcagt 13560 tcccagcctg ccaatgtgcc ccggggacag gaagactata ccaactttat tagcgccctg 13620 agactcatgg tagccgaggt tcctcagagc gaggtgtacc agtccggtcc agactacttt 13680 ttccagacaa gcaggaacgg tatgcagaca gtgaacttaa gccaggcttt caagaacctg 13740 caagggctgt ggggagtcca agctccagtg ggcgacaggg cgaccgtgtc gagcctgttg 13800 actccaaatt cccgtttgct gctgctgctg gtgtccccct tcactgacag cggcagcata 13860 aacagaaact cctacttggg ctacctgata aacttgtatc gcgaagctat aggtcaggcc 13920 cacgtggacg aacagaccta tcaggagatc actaatgtga gtcgcgctct gggccaggac 13980 gaccctggaa acctggaagc tactctaaac tttctgctga ccaaccgctc gcaaaaaatc 14040 cctcctcagt atacattaac tgcggaggag gaacggatct tgagatacgt gcagcagagc 14100 gtgggtctgt tcctgatgca agagggtgcg acccctagcg ccgcgcttga tatgacagcg 14160 cgcaacatgg agcccagcat gtatgccagc aacagaccat tcattaataa attgatggat 14220 tacttccatc gcgcggccgc tatgaactct gattacttca ccaatgctat tctgaacccc 14280 cattggctgc ctccgcctgg tttttatact ggcgagtatg acatgcctga ccccaacgat 14340 gggttcttgt gggacgatgt ggacagcgtg gcgttctcgc ctaccgctcc tcgtactttt 14400 tggaagaagg aaggtagtga cagaagaccc tcctccgtgc tgtcaggacg tgagggtgct 14460 gccgcggcgg tccccgatgc tgcaagcccc tttcccagtc tgccattttc actaaacagc 14520 gtgcgcagta gcgagctggg gagaataacc cgccctcgct tgctgggcga ggacgagtat 14580 ttgaatgact ccctactgag acccgagcgg gaaaagaact tccctaataa tgggattgaa 14640 agcctggtgg ataagatgag cagatggaag acctatgccc aggagcacag agatgagcct 14700 agaatcttgg gtcctacagt aggcacccgc agacgccagc gccatgatag acagcggggt 14760 ctggtgtggg acgatgagga ttctgcagat gacagcagcg tgttggactt gggcgggagg 14820 ggaggtgtgg gcaacccgtt cgcacacttg cgtccccgta ttggacgcat gatgtaaaag 14880 tgaaaataaa aaaggaactc accaaggcca tggcgaccag cgtgcgttcg ttctttctgt 14940 tgttgtatct agtatgatga ggcgcaccgt gctaggcgga tcggtggcgt atccggaggg 15000 tcctcctcct tcgtacgaaa gcgtgatgca gcaggtggcg gcggcggcga tgcaaccccc 15060 cttggaggct ccttacgtgc ccccgcggta cctggcacct accgagggga gaaacagcat 15120 tcgttattcg gaactcacac ccttgtatga caccacccgg ttgtacctgg tggacaacaa 15180 atcggcggac attgcctcgt tgaactatca gaacgaccac agcaacttct tgacaacggt 15240 ggtgcagaac aatgacttta cccccacgga ggccagcacc cagaccatca actttgacga 15300 gcgctcccgg tggggcggtc agctgaagac catcatgcac accaacatgc ccaacgtgaa 15360 cgagttcatg tttagcaaca agttcagggc tagggtgatg gtgtccagaa ccacacctaa 15420 agaggtgaca gtcacaacag actatgatgg tagtcaggac atcttggaat acgagtgggt 15480 tgactttgag ttaccagaag gcaacttctc tgccaccatg accatagacc tgatgaataa 15540 tgcaattgtt gataattacc taaaagtggg tagacagaat ggggtactgg agagtgacat 15600 aggtgttaag tttgacacta ggaactttag gcttggttgg gacccagtga cagagttggt 15660 catgcctggg gtctacacca atgaagcttt ccatcctgac atagtcctac tacctggctg 15720 cggagtggac ttcactgaga gccgcctcag taatctgcta ggcattagaa agaaacagcc 15780 attccaggaa gggttccaga tcatgtatga ggatctggag ggtggtaaca tccccgccct 15840 gcttgatgta aatgcatatg agaagagcaa ggaagataat acaaccacca caaatgaagc 15900 tgtggccgcg gcttcatcta ctgaagccaa agctgtggta gatgcttcca cttcaacaga 15960 aaacaccact gatgaaaaag tcaccagggg agatacattt gccacccctg aacaagagaa 16020 ggcagctgag gcagagtctg atattatgct tctgtccacc gatgaaaacg aaactaaaaa 16080 acaactggtt attcgagcgg tgaccaagga tagtaaggac aggagttata atgtattgtc 16140 agatggaaag aacacagctt accgtagttg gtacctggca tacaattatg gcgaccgtga 16200 gaaaggggtg cgttcttgga cactgcttac cacctcggat gtcacctgcg gcgtggagca 16260 agtctattgg tcgctaccag atatgatgca agatccagtc acctttcgct ccacacgcca 16320 agttagcaac tacccagtgg tgggcgcaga gctgctccca gtgcattcca gaagcttcta 16380 caacgagcaa gccgtctact cgcaacagct ccgccagtac acctcgctca cgcacgtctt 16440 caaccgcttc cccgagaatc agatcctcgt ccgcccgccc gcgccaacca ttaccaccgt 16500 cagtgaaaac gttcctgctc tcacagatca cgggaccctg ccgctgcgca gcagtatccg 16560 gggagtccag cgcgtgaccg ttactgacgc cagacgccgc acctgtccat acgtatacaa 16620 ggccctgggc atagtcgcgc cgcgcgtcct ttcaagccgc actttctaaa aaaatgtcca 16680 ttctcatctc gcccagtaat aacaccggtt ggggcctgcg cacacctagc aagatgtatg 16740 gaggcgctcg cagacgctcc actcagcacc ctgtgcgcgt gcgcgggcat ttccgcgctc 16800 cctggggcgc cctcaaggga cgctctcgta ctaggaccac cgttgacgat gtgatcgacc 16860 aggtggtcgc cgatgcacgt aactataccc ccgcagccgc acctgcatcc accgtggatg 16920 cggtcattga cagcgtggta gccgatgcgc gcgcctatgc tcgcgccaag agcaggaggc 16980 ggcgtattgc caggcgtcac cgagctactc cagccatgcg agctgcaaga gctttattgc 17040 ggagagccag acgtgtgggg cgaagagcca tgcgtagagc ggccagacgc gcggcttcag 17100 gtgccagcgc aggcagggtc cgcaggcgcg cggctacggc ggcagcggcg gccatcgcta 17160 gcatgaccaa accacgaaga ggcaatgtgt attgggtgcg cgacgccgcc accggccagc 17220 gcgtgcccgt gcgcacacgc ccccctcgca cttagaagat actgagcagt ctccgatgtt 17280 gtgtcccagc ggcgagatgt ccaagcgcaa attcaaggaa gagatgctcc aggtcatcgc 17340 gcctgagatc tacggtcctg cggtgaagga tgaaaaaaag ccccgcaaga tcaagcgggt 17400 caaaaaggac aaaaaggaag aagatggtga tgatgggctg gtggagtttg tgcgcgagtt 17460 tgccccaagg aggcgcgtgc agtggcgcgg gcgcaaagtg tggccggtgt tgagaccggg 17520 gaccacagtg gtctttacgc caggcgagcg ctccagcacc gtttccaaac gctcttatga 17580 tgaggtgtac ggggacgatg atattctcga gcaggcggct gatcgccttg gcgagtttgc 17640 atatggcaaa cgcagccgct cgggagccaa ggaagaggca ttgaccatcc ccttggatca 17700 tggaaatccc accccaagcc tcaaacccgt gaccctgcaa caagtgctgc ccacgccgcc 17760 acgcaagggc atcaagcgcg agggcgagga tctgtatccc accatgcagc tgatggtgcc 17820 caagcgccag aagctggaag acgtgctgga gaaaatgaaa gtggatcctg aaatccagcc 17880 tgaagtcaaa gtgaggccaa tcaagcaggt ggcgcccggt ttgggggtac aaaccgtgga 17940 tatcaagatc cccaccgagt ccatggaaat tcaaaccgaa cccatgaagc ccacctccag 18000 caccattgag gtgcagacgg atccttggat gcccgcgcct gctcctgtta ccactactac 18060 tcgaagacct agaagaaagt atggttcagc caacctgata atgccaaact atgctctgca 18120 tccatcaatc atacccactc ctggctaccg cggcactcgc tactaccgca gtcacagcac 18180 ccgccgacgt aaagcacctg ccacccgccg ccgtcgccgc cgccgtgcca ctagcaaact 18240 taccccctcg gctatggtgc ggagagtgta ccgtgatggg cgcgcagctc ctctgacact 18300 gccgcgcgcg cgctaccatc ctagcattgc catttaacaa ctctgcctcc ttgcagatat 18360 ggccctcact tgccgccttc gtattcctat tgctggctac cgcggaagaa agtcgcgccg 18420 tagaagagca gggttgtctg ggagcgggat gcgtcgccac cggcggcggc gcgccatcag 18480 caaacggttg gggggtggat ttcttcccgc tttgattccc atcatcgccg cggcgatcgg 18540 cgcgatacca ggcatagctt ccgtggcggt gcaggcctcg cagcgccact gacattggaa 18600 aaagatatct tataaataaa aatagaatgg actctgacgc tcctggtcct gtgatatgtt 18660 tttgtagacg agatggaaga catcaatttt tcatccctgg ctccgcgaca cggcacgcgg 18720 ccgtatatgg gcacctggag cgacatcggc aacagccaac tgaacggggg agccttcaat 18780 tggagcagtc tatggagcgg gcttaaaaat tttgggtcca ctataaagac ttatgggaac 18840 aaagcttgga acagcagcac agggcatgcg ctgagacaaa agcttaaaga tcagaatttc 18900 caacagaagg tggtcgatgg tatcgcctct ggaatcaatg gggtggtaga tctggccaac 18960 caggccgtgc agaaacagat taacagtcgc ctggacccgg ctcccccagc tcctattcat 19020 gagttaatgc aagtggagga agagctccct tcattggaaa agcggggcga taagcgacct 19080 cgtccagata tggaggaaac gctgctgacc aaggtggatg agccgccctc ctatgaagag 19140 gctgtaaaac tgggaatgcc cactacaaag cccattatgc ctctggccac tggagtgatg 19200 aagccatctc agtctaaacc tgcagttgct gctacattgg acttgcccgc tcccgtggcc 19260 acccccaaac ctgtcgccgc cccgaagccc accgccgtgc aacccgtggc cgtggccaga 19320 ccgcgtcccg gtggtcggcc gaatgcaaac tggcagagca ctctgaacag catcgtgggt 19380 ttgggagtgc acagtgtgaa gcgccgtcgc tgctattgat taaatatgga gtagcgctta 19440 acttgcttgt ctgtgtgtgt atatgtcgat gccgcccgcc gtgctacagc aaagagagaa 19500 ggagaagagg cgccgctgag ttcctttcaa gatggccacc ccatcgatgc tgccccagtg 19560 ggcgtacatg cacatcgccg gacaggacgc ttcggagtac ctgagtccgg gtctggtgca 19620 gttcgcccgc gccacagata cctacttcaa tctggggaac aagtttagga accctaccgt 19680 ggctcccacc cacgatgtga ccaccgaccg tagccagcgc ctgacgctgc gctttgtgcc 19740 cgttgaccgg gaggacaata cctactccta caaagtcaga tacaccctgg ctgtgggaga 19800 caacagggtg ttggatatgg ccagcaccta ctttgacatc aggggcgtgt tggacagagg 19860 acctagcttc aaaccatact ctggcactgc ctacaactcc ctggctccaa aaggagctcc 19920 aaactccagt cagtggcaac aaaaggaaaa caatggtcaa ggtgatgcaa agactcacac 19980 ctatggtgta gctgccactg gaggtattga cattgacaaa aatggtcttc aaattggaat 20040 cgatgaaact aaagaagata ataacgaaat ttatgcagac aaaacattcc aacctgaacc 20100 tcaaattgga gaagaaaact ggcaagatag cgaaaactat tatggaggca gggctcttaa 20160 accggaaacc aagatgaagc cttgctatgg ttccttcgct agaccaacta atgcaaaggg 20220 aggtcaagcc aaaattaaac cagctcaaga gggtcaacag tctatagatt atgacataga 20280 cctggctttc tttgatattc caagcactgg cggaggcaat ggcacaaatg taaatgacaa 20340 gccagatatg gttatgtata ctgaaaatgt aaatctggaa actccagaca ctcatcttgt 20400 ttacaagcca ggaacttcag atgacagttc cgaggccaat ttaactcagc aagccatggc 20460 taacagaccc aactatattg ggtttagaga taactttatt ggcgtcatgt actacaacag 20520 cactggcaac atgggagtgc ttgctggtca agcatcccag ctaaatgctg tggtggacct 20580 gcaagacaga aacaccgagc tgtcttatca gctattactt gactctctgg gcgacagaac 20640 caggtatttt agtatgtgga atcaggcggt ggacagctat gatcctgatg tgcgcattat 20700 tgaaaaccat ggtgtggaag atgaattgcc aaactattgc ttcccattgg acggagctgg 20760 cactaatgct gtttaccaag gagttaagac aaaagaggat aataatggcg aatgggaaac 20820 agacacaaat gttgcatcgc agaatcagat atgcaagggc aacatatatg ctatggagat 20880 caacctgcaa gccaacctgt ggaaaagttt cctttactcc aacgtggctc tgtacctacc 20940 agactcctac aagtacactc catccaacgt gacactccct accaacacta acacctatga 21000 ctacatgaat ggcagggtgg tgtctccatc cctggtggat gcctacatta acattggcgc 21060 caggtggtct ctggatgcca tggacaatgt caaccctttc aaccaccacc gcaatgccgg 21120 cctgcgctac cggtccatgc ttctgggcaa cggccgatac gtgcccttcc acatccaagt 21180 gccccagaaa ttcttcgcta tcaagaacct gctgcttctc ccaggctcat acacctacga 21240 gtggaacttc cgcaaggatg tcaacatgat cctgcagagt tcccttggca atgacctcag 21300 aaccgatggg gccaccatcc agtacaccag catcaatctc tatgccacct tcttccccat 21360 ggctcacaac actgcctcca ccctggaagc catgctgcgc aatgacacca atgaccagtc 21420 cttcaatgac tacctctcag ctgccaacat gctttacccc atccctgcca atgccaccaa 21480 cgtgcccatc tccatcccat ctcgtaactg ggctgccttc aggggctggt ctttcacccg 21540 cctcaagacc aaggagaccc catctctggg atcagggttc gatccctact tcgtctactc 21600 aggctccatt ccatacctgg atggaacttt ctaccttaac cacactttca agaaagtctc 21660 catcatgttt gactcttctg tcagctggcc aggcaatgac aggctgctga ctcccaatga 21720 gttcgaaatc aagcgcactg ttgatgggga agggtacaat gtggcacaat gcaacatgac 21780 caaagactgg ttcctggttc agatgctctc ccactacaac attggctacc agggcttcta 21840 catcccagaa ggatacaagg accgcatgta ctccttcttc agaaacttcc agcccatgag 21900 ccgccaggtg gtcgatcagg tcaactacaa agactacatg gcagtcaccc ttgcctatca 21960 gcacaacaac tctggctttg tgggctacct cgcgcccacc atgcgacagg gccaacccta 22020 ccctgctaac tacccatacc cgctcattgg caagactgca gtcaacagtg tcacccagaa 22080 aaagttcctc tgcgacaggg tcatgtggcg catccccttc tccagcaact tcatgtccat 22140 gggggccctt accgacctgg ggcaaaacat gctttatgcc aactccgccc acgcgctaga 22200 catgaatttc gaagtagacc ccatggatga gtccaccctt ctctatgttg tcttcgaagt 22260 cttcgacgtg gtcagagtgc accagcccca ccgcggcgtc atcgaagctg tctacctgcg 22320 cacccccttc tcagctggta acgccaccac ataagcgcct tgcttcttgc aagtggctgc 22380 agcagcatgg cctgtggatc ctccactgga tccaatgagc aagagctcag ggccatcgcc 22440 atagacctgg gctgtggacc ctatttcctg ggaacctttg acaagcggtt tccaggcttc 22500 atggctcctg acaagctcgc ctgtgccatt gtcaacacgg cagggcgcga gactggtggt 22560 gagcactggc tggcttttgg atggaacccc cgctccaata cctgctatct ctttgacccg 22620 tttgggtttt cagacgagcg cctcaagcag atctatcaat tcgagtacga ggggctcctg 22680 cgccgcagtg ccctggctac taaggaccga tgcatcactc tggaaaagtc tacccagacc 22740 gtgcagggtc cgcgctcggc tgcctgcggg ctcttctgct gcatgttcct ccatgctttt 22800 gtgcactggc ccgaccgccc catggacaac aaccccacca tgaatttgct gacgggggta 22860 cccaacaaca tgctccaatc gccccaagta gagcccaccc tgcgccacaa ccaggaggca 22920 ctctatcgct tcctgaactc ccactcatct tactttcgtt ctaaccgcgc gcgcattgag 22980 aaggccactg ccttcgatcg aatgaataat aacatgtaaa ccaaattgtg tgtggctcaa 23040 ataaacagca ctttattgtt tacatgcact gaggctctgg gatgatcatt ttttaaaaat 23100 cgaaggggtt ctggcgggaa tcagcatggc cagatggcag ggacacgttg cggaactgga 23160 acttgttctg ccacttgaac tcgggaatca ccagcctggg aactggaatc tctggaaagg 23220 tatcttgcca tagctttctg gtcagttgca gagcgccaag caggtcagga gcagatatct 23280 tgaaatcaca gttggggcca gaattctggg cgcgggagtt gcggtacact gggttgcagc 23340 actggaacac cataagggca gggtgtctca cgctcgccag cacggtctcg tcactgatgc 23400 aagacacatc caggtcttca gcattggcca ttccaaaggg ggtcatcttg caggtctgtc 23460 tgcccatcac gggagcgcag ccaggtttgt ggttgcaatc acaatgaagg gggatcagca 23520 tcatcttggc ctggtcgggg gtaatccctg ggtaaacagc cttcatgaag gcttcatact 23580 gcttgaaagc ttcctgggct ttggttccct cggtgtagaa cactccacaa gacttgctgg 23640 aaaactgatt agtagcgcag ttggcatcat tcacacagca gcgggcgtcg ttattagcca 23700 gctggaccac attcctgccc cagcggttct gggtgatctt ggctcgatct gggttctcct 23760 tcaacgcgcg ctggccgttc tcgctcgcca catccatctc aatgacatgt tccttctgga 23820 tcatgatgtt gccatgcagg catctaatct tgccttcata atcagtgcag ccatgaggcc 23880 acagcgcgca cccggtgcac tcccaattgt tatgggggat ctgggaatgg ctatgaacca 23940 gcccttgcag gaatcttccc atcatcacag ccagggtctt tatgctggta aaggtcagcg 24000 ggataccgcg gtgctcctcg ttcacatact gctggcagat gcgtctgtag tgctcggcct 24060 gctcgggcat cagcttgaaa gaggttttca actcattatc cagcctgtat ctctccatca 24120 tgatggacat tacttccatg cccttctccc aggcagaaac aatagggaga ctcaggggat 24180 tcttgacagt agagacaacc ttacttaagg ggtcatcact gccaatcttt tcgatgcttc 24240 tcttgccatc cttctcggtg atgcgcaccg gcgggtagct gaatcccaca gccaccaact 24300 gagcctcttc cctttcgtct tcgctgtctt gactgatgtc ttgcagagga acatgtttgg 24360 ttttcctggg tttcttcttg ggcggcagct ctggaggact ctggctccgt tccggagacc 24420 ccatggatga gcgagagttg tcgctcacca cttggatctg gctgcctgta gaagaactgg 24480 accccacgcg gcggtaggtg ttcctcttgg taggcagagg tggaggcgac gggctccggt 24540 ccggtctggg tggcggatgg ctggcggagc cccttccgcg ttcgggggtg cgctccagat 24600 ggcggtcgtc tgactgacct ccgcggctgg ccattgtgtt ctcctaggta gagaaacaag 24660 acatggagac tcagccatcg ctgccatcgc catccaccac cacaagcacc gccgaggagg 24720 aggagtgttt aaccacccca ccatgcagcc ccgctaccac caccagcacc cttgaaagcg 24780 aggtcgacac ggtcgtggag gatttacagg ctatggaaga tattgaggca gctgtcgagc 24840 aagaccccgg ctatgtgaca ccggcggagc atgatgagga tctagcgcgc tttctcgacg 24900 gtgtggagaa agcgaaacaa gatgaggacg aggaagaggc agaagcacaa ccatcggtgg 24960 ccgactacct caccggccta gggctagaag acgtgctgct taagcatctt gcaaggcaga 25020 cagtcatagt caaagacgcc ctgctagagc gctccgaggt gccactcagt gtggaagacc 25080 tcagtcgcgc ctatgagcta aacctcttct cgcctcgcaa gccccccaag cgtcagccca 25140 acgggacctg tgagcccaat ccgcgcctca acttctatcc agccttcact gtgcccgaag 25200 tactagctac ctaccacatc tttttcaaga accaaaagat ccccatctcc tgccgcgcca 25260 atcgcacccg cgcagatgcc ctactcaact tggggcccgg cgctcgcata cctgatatcg 25320 cttccttgga agaggttcct aagatctttg agggtctggg caatgaggaa actcgggcag 25380 caaacgctct gcaaagagaa acagatgatg gtgaacacca cagcgctctg gtggagctcc 25440 agggcgacaa cgctcgtctt gcagtcctca aacgcagcat cgaggtcacc catttcgcct 25500 accccgcact taatctccca cccaaagtca tgagctcggt catggacacg ttgctcatga 25560 agcgcgcgag ccccatctcc gaggatcaga acatgcagga ccccgatgcc tcagatgaag 25620 gcaagcctgt agtcagcgac gagcaactgg ctcgctggct aggctctgac tccccccagt 25680 ctttggagga gcggcgcaag cttatgatgg cagtggtcct gatcacagcg gagctggagt 25740 gtctccgccg cttcttcact gacccagaga ccctgcgcaa gcttgaggag aacctgcatt 25800 acacattcag tcatgggttc gtgcgccagg cgtgcaagat ctccaacgtt caactcacca 25860 acctggtctc ctacctgggc atcttgcatg aaaaccggct ggggcagaac gtgctccaca 25920 ccaccctgaa gggggaggcc cgccgcgact atatccgcga ctgtatctac ctctacctat 25980 gctacacctg gcaaagcggg atgggtgtgt ggcaacagtg cttggaagag caaaatctaa 26040 aagagctgga aaagctgctt cagaaatctc ttaaatctct gtggaccggg ttcgatgagc 26100 ggaccaccgc ttcggacatg gccgatatta tcttccccga gcggctcaga cacactctgc 26160 gcgacgggct gcctgacttt gccagccaga gcatgctaca aaactttagg tcattcatct 26220 tggaacgctc cgggatcctg cccgccactt gctgcgcact gccctccgat tttgtgccca 26280 tcacctaccg ggagtgcccc ccgccgctat ggagccactg ctacctgttc cgcctggcca 26340 actacttggc ctaccactct gatgtgatag aagatgttag tggcgaaggg ctcctggagt 26400 gccactgccg ctgcaacctc tgcacccccc accgctccct cgcctgcaat ccccagctgc 26460 tgagcgaaac ccagatcatc ggcaccttcg agttgcaagg tcccagcggc gaaggcgagg 26520 ggtcctctcc ggggcaaagt ctgaaactga ctccggggct atggacctcc gcttaccttc 26580 gcaagttcgc ccccaaagac taccacccct atgagatcag gttttatgaa gaccaatcac 26640 agccccccaa ggccgaactg acggcctgcg tcatcaccca gggggcaatc ttggcccaat 26700 tgcaagccat ccaaaaatcc cgccaagaat ttttgctgaa aaagggacac gggatctatc 26760 tagaccccca gaccggtgag gagctgaata cacgcttccc tcaggatgcc ccgaggaggc 26820 aagagaatga aagttcagat gccgcccgag gaggagctgg aagactggga cagtcaggca 26880 gaggaggaag actgggacag ccaggcagaa gaggaggaca gcctggagga ggacagtctg 26940 gaggaaggcg aggagcccaa ggaagaggca gccgccgcca gaccatcgtc ctcggcggtg 27000 gagacaagca aggtcccaga cagcacggct accacctccg ctccagctca aggggccgct 27060 cggcgaccca acagtagatg ggacgagacg ggtcgcttcc agaaccccac caccgtcaag 27120 accggtaagc aggagcggca gggatacaag tcctggcggg ggcataaaag tgccatcatc 27180 gcttgcttgc aggagtgtgg gggcaatata tcctttgcca gacgctacct gctattccat 27240 cacggggtga atttcccccg caacatcttg cattactacc gtcacctcca cagcccctac 27300 taccagcagc aagagacagc agaggaaacc agcggcaact ccgagagtta gaaaaccagc 27360 agctaaaaaa tccacagcgg cggcagcagg tgcaggcgga ctgaggatca ccgcgaacga 27420 gccagctcag accagggagt tgaggaatcg gatctttccc accctctatg ccatattcca 27480 acaaagtcgg ggtcaggaac aagaactgaa agtaaaaaac agatctcttc gctcgctcac 27540 ccgcagttgt ttgtatcaca agagcgaaga ccaacttcag cgcactctcg aggacgccga 27600 ggctctcttc aacaagtact gcgcgctgac tcttaaagag tagactgcgc gcgcttggcg 27660 agaaaaggcg ggaattacgt cacctcttgg ccacacctgt gcttcattat gagtaaagaa 27720 attcccacgc cttacatgtg gagctatcag ccccagatgg gattggccgc tggcgccgcc 27780 caggactact ccacccgcat gaattggctc agcgccggtc ccgcgatgat ctcacgggtt 27840 aatggtgtga gagagcaccg aaaccagata ctcctagaac agtccgccct caccgccact 27900 ccccgcaatc acctcaaccc ccgtaattgg cccgccgccc tggtgtacca ggaaactcct 27960 gctcccacta cagtactact tcctcgtgac gcccaggccg aagttcagat gactaactca 28020 ggtgtacagc tggcgggtgg tgccaccctg tgtcgtcacc ggccaagacc gggtataaag 28080 ggcctggtga tcagaggccg aggtattcag ctcaacgacg agtcggtgaa ctcttcgctt 28140 ggtctgcgac cagacggcat cttccaaata gctggttgtg ggagatcttc cttcactcct 28200 cgtcaggctg tcctgacttt ggagagttcg tcctcgcagc cccgctcggg cggcatcggg 28260 actctccagt tcgtggagga gtttactccc tcggtctact tcaacccctt ctccggttct 28320 cctgggcttt acccggacga gttcatcccg aactacgacg ccatcagtga agcggtcgac 28380 ggctacgatt aatgtctaat ggtggcgcgg ctgagctagc tcgactgcga cacctagacc 28440 actgccggcg ctttcgctgc tttgctcggg atctctgcga gttcatctac ttcgagtacc 28500 ctgacgaaca tcctcaggga cctgcccacg gagttcggat taccattgaa ggggctatcg 28560 attctcacct gcttcggatc ttcaccgctc ggccagtgct agttgagcgc aaccagggcg 28620 acaccaccat ctccctctgc tgcatttgtg acaaccccgg attgcatgaa agcttttgtt 28680 gtcttctttg tactgagtat aataaaagct gaaattagag actactccgg actctcttgt 28740 cgtctgaaca acaccaacca gacccttcac ttcagcggga accagactac tcttcactgt 28800 aaggcttata actataagta tcttacttgg atatacaaag gaacaccgtt tgctgtggta 28860 aacaggtgct ccaacgacgg tgttctcctc accttcctag gcaacttctc caactttacc 28920 ttttctgttc gcagaaacaa gcttaccctc cttcagccct actttcctgg gatctatacc 28980 tgcctcagtg gaccttgcaa ccacactttt cacctgattg aaaactctac ccttaccttc 29040 ccagcgccaa tccctactaa cagctcggag tccaactctt ccattaccgc tgatactaac 29100 actcctaaaa ccggaggtga gctccgcagc cttcccccgg ctgcagataa cccttgggtg 29160 gtagcgggat ttgtagcgct aggaatagtt gcgggtgggc tcgcgttcgt cctctgctac 29220 ctatacctta cctgctgctc atatttagta gtactgtgct gttggtttag aaaatggggg 29280 cgctactaat cacacttgct ttactttcgc ttttgggtct gagctcggct aatagcgaga 29340 aaccaagctg tctagaaaca aactctccag actgtgtggt tcctcatggg ctctcagacc 29400 cagctgatga tccatgctta acttttgacc cagaaaaaaa ctgctcggtg actatgcagc 29460 cctatgctta catgtgcaca tctgttataa agtgcggatg gggctgtaaa ccgattgaaa 29520 ttacccacaa aggcaaaacc tggaataata gtttgtttaa cacatggcag cctggagacg 29580 agcagtggta tacggccggc cactggtgga gatgactgac cccatggaaa actcctctgc 29640 caacgacctg gacatggacg gccgttcatc tgagcagcga ctggtccaga tgcgcattcg 29700 ccagaagcag gaacgcgccg ccagagagct caaggatgcc attgaaattc acctgtgcaa 29760 gaagggcatc ttttgcttgg ttaagcaagc aaagatttct tatgaaatca ctgacaacga 29820 ccaccgcctg tattatgagc tcggtccaca gcggcagaaa ttcacctgca tggttggagt 29880 caaccccata gtcatcactc agcaggctgc agaaattaaa gggtgcatcc actgttcctg 29940 tgattcccaa gaatgcgtcc acaccatagt caagaccctc tgcggccttc gagatcttct 30000 tccaatgaac taaccccttc ccccaaccca ataaaacatt ggttttaatc ataataaaaa 30060 atcacttact ttaaatctga aacagtgtct ccgtccaagt tttcttgtag caccacttca 30120 ctcccctctt cccagctctg gtactgcaag ccccggtggg ctgcaaactt tctccacacc 30180 ttaaaaggga tgtcaaattc ctcttgtcca acaatcttca ttgtctcttc ctagatgtcc 30240 acaaagcgcg cgcgggtgga agatgacttt gaccctgtct acccatacga tgctgagctg 30300 gcaccgtctg tacccttcat cgcccctccc ttcgtttcgt cagacggatt tcaagaaaaa 30360 cccctgggag ttctgtccct aagactagcc aacccagtca ctactaaaaa tggggaactc 30420 acacttaaac tgggagatgg ggtgggcata gactcagatg gaaacctcac agcacagaca 30480 gttactaaag caacatcccc ccttactgtt tccaataacg caattgcact taacatggac 30540 aaaccttttt acagtagcaa tggaaaacta tccttacaag ttacatcacc attaaagata 30600 gtcgactctt taaatacatt ggctattggc tatgggcaag gcttaggact aaacaatagt 30660 gctcttgctg tgcaattagc atctcccctt acatttgaca gcaacagcaa aattaaaata 30720 aatttgggaa gcgggccatt aaaaattaat gcgaataaac tgtcaattaa ctgcctaagg 30780 ggtgtatatg taacaactga cggaacttcc attgaaacaa atataagctg ggcaaaagga 30840 atgaggtttg aaggtaatgc catggctgta aacgttgaca gcaccaaagg tctacaattt 30900 ggcactacca gcacagaatc aggagtcact aacgctttcc ctatccagtt aaagattgga 30960 tctggtctta gttttgacag cacaggagca cttgtagctt gggataagga taatgacaag 31020 cttacactgt ggacaaccgc tgacccatca cctaattgta ccatatatac agacaaggat 31080 gctaaactta cactttgtct tacaaaatgt ggcagtcaaa tactaggcag tgtttcagta 31140 ctggctgtta aagctggaac cctacagcca atcagtgaaa aaataggtac tgctttggtt 31200 tcactaaaat ttaataacaa cggtgtattg ttaagcaact ccacattaag taatgaatac 31260 tggaactaca ggaagggaga tgtcacacca gccgaagcct atactaatgc tgtgggtttt 31320 atgccaaaca tcaaggcata tcctaaaaac acaaactctg cctcaaaaag ccacattgta 31380 ggacaagtgt accttaatgg agatgaaact aaaccaatgc atttaatcat tacatttaat 31440 gaaaccagtg atgaaacatg cacatattcc ataacgttcc aatggaaatg gaacattgga 31500 acatacacca gcgacaccct tgcaacaagc tcctttacct tttcttacat tgcccaagaa 31560 taaaaactgc agacaacaat aaagtttaaa tgttttattt aaacagtttc acagaaccct 31620 agtattcaac ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct 31680 ggccttaaaa agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac 31740 ggtttcctgt cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact 31800 taagttcatg tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt 31860 aacgggcggc gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag 31920 gatagggcgg tggtgctgca gcagcgcgcg aataaactgc tgccgccgcc gctccgtcct 31980 gcaggaatac aacatggcag tggtctcctc agcgatgatt cgcaccgccc gcagcataag 32040 gcgccttgtc ctccgggcac agcagcgcac cctgatctca cttaaatcag cacagtaact 32100 gcagcacagc accacaatat tgttcaaaat cccacagtgc aaggcgctgt atccaaagct 32160 catggcgggg accacagaac ccacgtggcc atcataccac aagcgcaggt agattaagtg 32220 gcgacccctc ataaacacgc tggacataaa cattacctct tttggcatgt tgtaattcac 32280 cacctcccgg taccatataa acctctgatt aaacatggcg ccatccacca ccatcctaaa 32340 ccagctggcc aaaacctgcc cgccggctat acactgcagg gaaccgggac tggaacaatg 32400 acagtggaga gcccaggact cgtaaccatg gatcatcatg ctcgtcatga tatcaatgtt 32460 ggcacaacac aggcacacgt gcatacactt cctcaggatt acaagctcct cccgcgttag 32520 aaccatatcc cagggaacaa cccattcctg aatcagcgta aatcccacac tgcagggaag 32580 acctcgcacg taactcacgt tgtgcattgt caaagtgtta cattcgggca gcagcggatg 32640 atcctccagt atggtagcgc gggtttctgt ctcaaaagga ggtagacgat ccctactgta 32700 cggagtgcgc cgagacaacc gagatcgtgt tggtcgtagt gtcatgccaa atggaacgcc 32760 ggacgtagtc attctcgtac tttgagtggc aaaaccttgc tctcgaacag cacacgtctc 32820 gtcgcctcct gtcccttctc ttcgcctttt cagtgtgata gttgtaatac agccattcac 32880 gaagctcagt cagaagatct tcagcgtctg ttgtcaaaaa caatccatcc aatctgattg 32940 ctttcaaaac atcacaaaca gtcgaataag ccaaacccat ccaggcaatg caattatttt 33000 ggttatccac aatgggaggg ggcggaagac atggaagagg cataattaat tttttaatcc 33060 aatcgatcac gcagcacttc aaaatgaaga tcgcgaaggt gacacctttc acccccactg 33120 tgttgatgaa aaataacagc caagtcaaaa ttgatgcggt tttcaaggtg ctcgactgta 33180 gcatcaagca gagcttccac acgcacgtcc acaaataaca gaatagcaaa agcgggagga 33240 ggaagtaaat cctcaatcat catagtacag tccatcacca tccctaaata attttcatcc 33300 ttccagcctt ggactatatt tttaaactgc tcttgtaaat ccaaaccaca catgtggaaa 33360 agttcccaaa gagctccctc aactaccatt cttaagcaca ccttcatagt gacaaaatat 33420 cttgttcctc tgtcacctgc agcaaattac aaagtccaat attaggatct atgcccagag 33480 atctaagctc atccctcaat tccaactgta aaaaggcttc cagatctgcc ctaacttgtt 33540 cagccagtgg gctccctgga ataagcgtgg gagaagccaa actgcaaaac agacgcatgc 33600 cgccataatt accaccagaa aacactacgt tacagtatgc atgctgattc attccagtaa 33660 tttcatccag tgtattggat acaaaaaaag gcaagcactc tctcactaat tgtattatgg 33720 agacattatc acacaggtaa caatttaaag gttgtggaac aataatgcag taagtaacca 33780 cggtgcgctc caacatggtt agtaattttt agttctgaaa aacaaaacat acaaaaaatt 33840 atatcatact catttggcga actggtggaa aaatgaccct atctagcaca aggcaagcca 33900 ctggatcacc aatgcgcccc tcataaaacc tgtcatcatg attaaaaagc aacaccgaaa 33960 gctcttccct atgtcctgca tgaatgattc tagctgagga atataagcca gcgcaattag 34020 tatctgttaa agaaaaaaaa cggccaacat agcctctagg aattagcaca cttaatctta 34080 aagacattac tgccatcccc cttggattta aggtaaaatt tacaggagca tagaaaatat 34140 actgatttcc ctcctgcaca ggcagcatag caccaggtcc ctctaaaaac acacacaaag 34200 cttctgcagc catagcttac cgcgcaaacc aggcacagca gtgagctaaa aggacaaagc 34260 tctaactcac tagccaacct ggcgcacaat atatagttag tccttacact gacgtaaccg 34320 accaaagtct aaaaaccccg ccaaaaatac acacacgccc aaaaaacgcc ccgtgagtca 34380 aaaaacagtt tcacttcctc gttacaccca aaacgtcgtc acttccggat tcccacggtt 34440 cgtcacttcc ggagctcctt gcttaattaa ccccgcccaa aacgtcatcg tccgcgtcac 34500 gccgccccgc cccgcgaccg ttgaccccgg gccaatcacc gcacatcccg caaaattcaa 34560 actcgtctaa tttgcatatt ggcacactgc ccatataagg tatattattg atgatgattt 34620 aaatcatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 34680 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 34740 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 34800 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 34860 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 34920 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 34980 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 35040 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 35100 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 35160 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 35220 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 35280 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 35340 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 35400 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 35460 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 35520 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 35580 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 35640 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 35700 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 35760 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 35820 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 35880 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 35940 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 36000 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 36060 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 36120 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 36180 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 36240 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 36300 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 36360 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 36420 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 36480 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 36540 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 36600 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 36660 gcgtatcacg aggccctttc gtcttcaaga attgatttaa at 36702 <210> 22 <211> 33625 <212> DNA <213> Artificial Sequence <220> <223> BLY6.FLuc Ad vector <400> 22 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cggcgatcgc tcaatattgg ccattagcca 480 tattattcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt 540 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac 600 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 660 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 720 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 780 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 840 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 900 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 960 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 1020 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 1080 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 1140 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 1200 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 1260 gcctccgcgg ccgggaacgg tgcattggaa gcttggcatt ccggtactgt tggtaaagcc 1320 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 1380 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 1440 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 1500 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 1560 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 1620 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 1680 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 1740 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 1800 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 1860 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 1920 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 1980 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 2040 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 2100 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 2160 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 2220 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 2280 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 2340 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 2400 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 2460 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 2520 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 2580 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 2640 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 2700 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 2760 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 2820 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 2880 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 2940 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agacgagatc cgaacttgtt 3000 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 3060 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 3120 ctgcgatcgc gatggcctgt gtttgagcat aatgtactga ccaggtgtaa cgttcatctg 3180 ggtggtcgta gaggaatgtt catgccatac caatgcaatt ttaatcatgt gaggatcttg 3240 atggagccgc aagcgttttc cagagtcagc ttgactggaa tctttgacat gtgtgtggaa 3300 gcatggaaga tcttaagata tgatgatacc aaatccagat gccgcgcatg cgagtgcggg 3360 ggcaggcatg ccaggttcca acctgtatgt gtggaggtga ccgaggagct gagaccagat 3420 catttggtgc tgacctgcac tggtgcggag ttcggttcca gtggtgaaga aactgattaa 3480 agtgagtagt gggatgttat aaaagtgacc ataaggtgat gtgagatgga caaatttggt 3540 aatttttatg tatttttgtc ttgcagccat gagtgggagc gcttcctttg aagggggcgt 3600 ctttagccct tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat 3660 ggggtctaca gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc 3720 tactatgact tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa 3780 cactgttcgg gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac 3840 taccccaaat aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct 3900 ggagtctttg acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca 3960 gactcaagct gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat 4020 aaaccgagac tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat 4080 atgccctgga ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat 4140 agaggtggga ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc 4200 tccactgcag agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc 4260 gttgggcatg atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct 4320 tggtgtaagt gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt 4380 gcattttgga ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca 4440 tgttatgcaa gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt 4500 tggaggggaa agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc 4560 actcatccat aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg 4620 gatctgacac atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact 4680 tggggcggag ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc 4740 cttcacatat ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg 4800 gagcgatgaa gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc 4860 taagcagctg tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca 4920 gatggtagtt tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca 4980 tcatttccct cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca 5040 gggaaaggag ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg 5100 gcattttgga gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt 5160 ctatggcatc tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta 5220 tggaaccagg cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt 5280 ccgagtcagg gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag 5340 ggtgcgcttc aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc 5400 ggccaggtag caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc 5460 gcgcagctta cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata 5520 gagttttggg gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca 5580 gacggtttcg cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt 5640 tccgccatgt tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg 5700 ggtgacaaag aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg 5760 cgtgccgcgg tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca 5820 ggccagcaca aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac 5880 tttttccaag gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt 5940 gtaagtgtat gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct 6000 ctgctcgtcc tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta 6060 ttccctctcg aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga 6120 ggatttgata ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc 6180 agaaaagaca atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga 6240 aagcagtttg gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc 6300 ggctatgttg agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt 6360 taattcatct gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac 6420 actggttgcc acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg 6480 agaacagaaa gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt 6540 gaaaatccca ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga 6600 catttgccat tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg 6660 catgggatgg gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc 6720 ttccagaatc cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata 6780 atcatagagt tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt 6840 ttctgctctg tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct 6900 ttggaagatg ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata 6960 ggagtcttgc agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc 7020 gagggtctct ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt 7080 gagaaggtat tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga 7140 acggtaagaa cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc 7200 cacggggagt gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt 7260 gtccctgacc atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg 7320 ctcccacagt tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac 7380 atcgttgaat aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg 7440 aggcacttct gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt 7500 gatgttgtga cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag 7560 ttttttaagc tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc 7620 ccagtcaggc aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc 7680 tgtttgtaag cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt 7740 aacacagtaa aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag 7800 gtcgtgggcg aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg 7860 gacaagctgc ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa 7920 gagcctttct gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt 7980 ggaggaatgg ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg 8040 cttgtgcttg tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat 8100 gagctgtacc tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg 8160 catctcgtgt tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt 8220 catgctgacg agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc 8280 gaggacgaga gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc 8340 agtagggaga gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag 8400 atgatatttg atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg 8460 cccctggggt gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg 8520 catggtaagg tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt 8580 tctggacctg gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg 8640 tactgcgccc tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga 8700 cgcctctggg tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa 8760 tcaatttcgg tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg 8820 tcttggtagg cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg 8880 cccgctcgtt ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat 8940 gcattcatgc ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg 9000 gcgcgcatga ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg 9060 cagagacgct ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac 9120 atgatccagc gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg 9180 gcttcgtaaa agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac 9240 tcctcctcca gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct 9300 cccgggatct cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact 9360 tcctcctcag gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc 9420 agacgatcga tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg 9480 gcgcgcccgt cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg 9540 tgactgggag ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc 9600 cctgtaggga ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc 9660 tgaacgaagg cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg 9720 cgggctgctg agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt 9780 ctgagacggc ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc 9840 aggcggtcgg ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct 9900 tgcatgagtc gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt 9960 ccgtaccctc tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg 10020 gcttgctgca cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag 10080 gcccccgtgt tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt 10140 cctggtcgta cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa 10200 tcgttgcagg ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg 10260 tagagaggcc atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg 10320 tggtatccgt agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc 10380 ggaaactctc gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc 10440 acggtctggc ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa 10500 gcgttgagcg gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac 10560 cctggttcga gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta 10620 ttggcaatcc cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg 10680 tttttcaggg catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt 10740 ggctcgcgcc cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt 10800 cgagcctgca gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg 10860 tttctaagac cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt 10920 tttgtttttg ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc 10980 agcagcaggc tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg 11040 ctgtaagcgg ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg 11100 agggattggc aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa 11160 aggacgttcg cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg 11220 agcccgagga gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg 11280 atcgaaaacg ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc 11340 ccgctcgcgc tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg 11400 aggaaagcaa cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg 11460 aggtgaccct gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca 11520 ctagcaaacc cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg 11580 cattcagaga ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg 11640 tgaatatcct gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg 11700 cggcgatcaa ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa 11760 ccccttacgt gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc 11820 tgaaagtgct aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg 11880 cggtgagcgc cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac 11940 gagccctgac gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc 12000 attggctgcc tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag 12060 tagtagacga ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt 12120 tttgctagat ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt 12180 ccggcattaa ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga 12240 cccgcaaccc cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg 12300 aggccgtggt gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg 12360 ccctggtgga gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc 12420 tcgagcgcgt ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga 12480 ccgacgtgcg cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt 12540 ccatggtagc gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg 12600 aagactatac caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg 12660 aggtgtacca gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag 12720 tgaacttaag ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg 12780 gcgacagggc gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg 12840 tgtccccctt cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa 12900 acttgtatcg cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca 12960 ctaatgtgag tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact 13020 ttctgctgac caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg 13080 aacggatctt gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga 13140 cccctagcgc cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca 13200 acagaccatt cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg 13260 attacttcac caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg 13320 gcgagtatga catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg 13380 cgttctcgcc taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct 13440 cctccgtgct gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct 13500 ttcccagtct gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc 13560 gccctcgctt gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg 13620 aaaagaactt ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga 13680 cctatgccca ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca 13740 gacgccagcg ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg 13800 acagcagcgt gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc 13860 gtccccgtat tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat 13920 ggcgaccagc gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg 13980 ctaggcggat cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag 14040 caggtggcgg cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac 14100 ctggcaccta ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac 14160 accacccggt tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag 14220 aacgaccaca gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag 14280 gccagcaccc agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc 14340 atcatgcaca ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct 14400 agggtgatgg tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt 14460 agtcaggaca tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct 14520 gccaccatga ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt 14580 agacagaatg gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg 14640 cttggttggg acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc 14700 catcctgaca tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt 14760 aatctgctag gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag 14820 gatctggagg gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag 14880 gaagataata caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa 14940 gctgtggtag atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga 15000 gatacatttg ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt 15060 ctgtccaccg atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat 15120 agtaaggaca ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg 15180 tacctggcat acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc 15240 acctcggatg tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa 15300 gatccagtca cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag 15360 ctgctcccag tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc 15420 cgccagtaca cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc 15480 cgcccgcccg cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac 15540 gggaccctgc cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc 15600 agacgccgca cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt 15660 tcaagccgca ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg 15720 gggcctgcgc acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc 15780 tgtgcgcgtg cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac 15840 taggaccacc gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc 15900 cgcagccgca cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg 15960 cgcctatgct cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc 16020 agccatgcga gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat 16080 gcgtagagcg gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc 16140 ggctacggcg gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta 16200 ttgggtgcgc gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac 16260 ttagaagata ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa 16320 ttcaaggaag agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat 16380 gaaaaaaagc cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat 16440 gatgggctgg tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg 16500 cgcaaagtgt ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc 16560 tccagcaccg tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag 16620 caggcggctg atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag 16680 gaagaggcat tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg 16740 accctgcaac aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat 16800 ctgtatccca ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag 16860 aaaatgaaag tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg 16920 gcgcccggtt tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt 16980 caaaccgaac ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg 17040 cccgcgcctg ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc 17100 aacctgataa tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc 17160 ggcactcgct actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc 17220 cgtcgccgcc gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac 17280 cgtgatgggc gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc 17340 atttaacaac tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt 17400 gctggctacc gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg 17460 cgtcgccacc ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct 17520 ttgattccca tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg 17580 caggcctcgc agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga 17640 ctctgacgct cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt 17700 catccctggc tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca 17760 acagccaact gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt 17820 ttgggtccac tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc 17880 tgagacaaaa gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg 17940 gaatcaatgg ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc 18000 tggacccggc tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt 18060 cattggaaaa gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca 18120 aggtggatga gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc 18180 ccattatgcc tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg 18240 ctacattgga cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca 18300 ccgccgtgca acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact 18360 ggcagagcac tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct 18420 gctattgatt aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg 18480 ccgcccgccg tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag 18540 atggccaccc catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct 18600 tcggagtacc tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat 18660 ctggggaaca agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt 18720 agccagcgcc tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac 18780 aaagtcagat acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac 18840 tttgacatca ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc 18900 tacaactccc tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac 18960 aatggtcaag gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac 19020 attgacaaaa atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt 19080 tatgcagaca aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc 19140 gaaaactatt atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt 19200 tccttcgcta gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag 19260 ggtcaacagt ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc 19320 ggaggcaatg gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta 19380 aatctggaaa ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc 19440 gaggccaatt taactcagca agccatggct aacagaccca actatattgg gtttagagat 19500 aactttattg gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa 19560 gcatcccagc taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag 19620 ctattacttg actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg 19680 gacagctatg atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca 19740 aactattgct tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca 19800 aaagaggata ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata 19860 tgcaagggca acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc 19920 ctttactcca acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg 19980 acactcccta ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc 20040 ctggtggatg cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc 20100 aaccctttca accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac 20160 ggccgatacg tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg 20220 ctgcttctcc caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc 20280 ctgcagagtt cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc 20340 atcaatctct atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc 20400 atgctgcgca atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg 20460 ctttacccca tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg 20520 gctgccttca ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga 20580 tcagggttcg atccctactt cgtctactca ggctccattc catacctgga tggaactttc 20640 taccttaacc acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca 20700 ggcaatgaca ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa 20760 gggtacaatg tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc 20820 cactacaaca ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac 20880 tccttcttca gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa 20940 gactacatgg cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc 21000 gcgcccacca tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc 21060 aagactgcag tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc 21120 atccccttct ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg 21180 ctttatgcca actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag 21240 tccacccttc tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac 21300 cgcggcgtca tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca 21360 taagcgcctt gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat 21420 ccaatgagca agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg 21480 gaacctttga caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg 21540 tcaacacggc agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc 21600 gctccaatac ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga 21660 tctatcaatt cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat 21720 gcatcactct ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc 21780 tcttctgctg catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca 21840 accccaccat gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag 21900 agcccaccct gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt 21960 actttcgttc taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata 22020 acatgtaaac caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg 22080 aggctctggg atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc 22140 agatggcagg gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac 22200 cagcctggga actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag 22260 agcgccaagc aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc 22320 gcgggagttg cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac 22380 gctcgccagc acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat 22440 tccaaagggg gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg 22500 gttgcaatca caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg 22560 gtaaacagcc ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc 22620 ggtgtagaac actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt 22680 cacacagcag cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg 22740 ggtgatcttg gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac 22800 atccatctca atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt 22860 gccttcataa tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt 22920 atgggggatc tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc 22980 cagggtcttt atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg 23040 ctggcagatg cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa 23100 ctcattatcc agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca 23160 ggcagaaaca atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg 23220 gtcatcactg ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg 23280 cgggtagctg aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg 23340 actgatgtct tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc 23400 tggaggactc tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac 23460 ttggatctgg ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt 23520 aggcagaggt ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc 23580 ccttccgcgt tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc 23640 cattgtgttc tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc 23700 atccaccacc acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc 23760 cgctaccacc accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc 23820 tatggaagat attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca 23880 tgatgaggat ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga 23940 ggaagaggca gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga 24000 cgtgctgctt aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg 24060 ctccgaggtg ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc 24120 gcctcgcaag ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa 24180 cttctatcca gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa 24240 ccaaaagatc cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt 24300 ggggcccggc gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga 24360 gggtctgggc aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg 24420 tgaacaccac agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa 24480 acgcagcatc gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat 24540 gagctcggtc atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa 24600 catgcaggac cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc 24660 tcgctggcta ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc 24720 agtggtcctg atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac 24780 cctgcgcaag cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc 24840 gtgcaagatc tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga 24900 aaaccggctg gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta 24960 tatccgcgac tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg 25020 gcaacagtgc ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct 25080 taaatctctg tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat 25140 cttccccgag cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag 25200 catgctacaa aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg 25260 ctgcgcactg ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg 25320 gagccactgc tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga 25380 agatgttagt ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca 25440 ccgctccctc gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga 25500 gttgcaaggt cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac 25560 tccggggcta tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta 25620 tgagatcagg ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt 25680 catcacccag ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt 25740 tttgctgaaa aagggacacg ggatctatct agacccccag accggtgagg agctgaatac 25800 acgcttccct caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg 25860 aggagctgga agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag 25920 aggaggacag cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag 25980 ccgccgccag accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta 26040 ccacctccgc tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg 26100 gtcgcttcca gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt 26160 cctggcgggg gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat 26220 cctttgccag acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc 26280 attactaccg tcacctccac agcccctact accagcagca agagacagca gaggaaacca 26340 gcggcaactc cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt 26400 gcaggcggac tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg 26460 atctttccca ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa 26520 gtaaaaaaca gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac 26580 caacttcagc gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact 26640 cttaaagagt agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc 26700 cacacctgtg cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc 26760 cccagatggg attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca 26820 gcgccggtcc cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac 26880 tcctagaaca gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc 26940 ccgccgccct ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg 27000 cccaggccga agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt 27060 gtcgtcaccg gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc 27120 tcaacgacga gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag 27180 ctggttgtgg gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt 27240 cctcgcagcc ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct 27300 cggtctactt caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga 27360 actacgacgc catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc 27420 tgagctagct cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga 27480 tctctgcgag ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg 27540 agttcggatt accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg 27600 gccagtgcta gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga 27660 caaccccgga ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg 27720 aaattagaga ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact 27780 tcagcgggaa ccagactact cttcactgta aggcttataa ctataagtat cttacttgga 27840 tatacaaagg aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca 27900 ccttcctagg caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc 27960 ttcagcccta ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc 28020 acctgattga aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt 28080 ccaactcttc cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc 28140 ttcccccggc tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg 28200 cgggtgggct cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag 28260 tactgtgctg ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct 28320 tttgggtctg agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga 28380 ctgtgtggtt cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc 28440 agaaaaaaac tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa 28500 gtgcggatgg ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag 28560 tttgtttaac acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag 28620 atgactgacc ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct 28680 gagcagcgac tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc 28740 aaggatgcca ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca 28800 aagatttctt atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag 28860 cggcagaaat tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca 28920 gaaattaaag ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc 28980 aagaccctct gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa 29040 taaaacattg gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc 29100 cgtccaagtt ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc 29160 cccggtgggc tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa 29220 caatcttcat tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg 29280 accctgtcta cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct 29340 tcgtttcgtc agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca 29400 acccagtcac tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag 29460 actcagatgg aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt 29520 ccaataacgc aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat 29580 ccttacaagt tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct 29640 atgggcaagg cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta 29700 catttgacag caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg 29760 cgaataaact gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca 29820 ttgaaacaaa tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa 29880 acgttgacag caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta 29940 acgctttccc tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac 30000 ttgtagcttg ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac 30060 ctaattgtac catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg 30120 gcagtcaaat actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa 30180 tcagtgaaaa aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt 30240 taagcaactc cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag 30300 ccgaagccta tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca 30360 caaactctgc ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta 30420 aaccaatgca tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca 30480 taacgttcca atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct 30540 cctttacctt ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat 30600 gttttattta aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac 30660 acagagtaca cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca 30720 gacatattct taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg 30780 atattaataa actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc 30840 acaggctgct gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac 30900 atgggggtag agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga 30960 ataaactgct gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca 31020 gcgatgattc gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc 31080 ctgatctcac ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc 31140 ccacagtgca aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca 31200 tcataccaca agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac 31260 attacctctt ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta 31320 aacatggcgc catccaccac catcctaaac cagctggcca aaacctgccc gccggctata 31380 cactgcaggg aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg 31440 atcatcatgc tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc 31500 ctcaggatta caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga 31560 atcagcgtaa atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc 31620 aaagtgttac attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc 31680 tcaaaaggag gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt 31740 ggtcgtagtg tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca 31800 aaaccttgct ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc 31860 agtgtgatag ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt 31920 tgtcaaaaac aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc 31980 caaacccatc caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca 32040 tggaagaggc ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat 32100 cgcgaaggtg acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat 32160 tgatgcggtt ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca 32220 caaataacag aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt 32280 ccatcaccat ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct 32340 cttgtaaatc caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc 32400 ttaagcacac cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca 32460 aagtccaata ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa 32520 aaaggcttcc agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg 32580 agaagccaaa ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt 32640 acagtatgca tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg 32700 caagcactct ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg 32760 ttgtggaaca ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta 32820 gttctgaaaa acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa 32880 aatgacccta tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct 32940 gtcatcatga ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct 33000 agctgaggaa tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata 33060 gcctctagga attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa 33120 ggtaaaattt acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc 33180 accaggtccc tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca 33240 ggcacagcag tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata 33300 tatagttagt ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca 33360 cacacgccca aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa 33420 aacgtcgtca cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac 33480 cccgcccaaa acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg 33540 ccaatcaccg cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc 33600 catataaggt atattattga tgatg 33625 <210> 23 <211> 34616 <212> DNA <213> Artificial Sequence <220> <223> BLY6.RSVF-2A-GLuc Ad vector <400> 23 catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60 attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120 cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180 cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240 aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300 tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360 ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420 ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgct caatattggc cattagccat 480 attattcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta 540 tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca 600 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 660 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 720 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 780 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 840 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 900 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 960 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 1020 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 1080 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 1140 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat 1200 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag 1260 cctccgcggt tggccgtgct cttcctgacg ggtaggtgtc ccctaaccta gggagccaac 1320 catcgggggg ccttctccct aaatccccgt ggcccaccct cctgggcaga ggcagcaggt 1380 ttctcactgg ccccctctcc cccacctcca agcttggcct ttcggctcag atctcagccc 1440 acagctggcc tgatctgggt ctcccctccc accctcaggg agccaggctc ggcatttcgt 1500 cgacatggaa ctgctgatcc tgaaggccaa cgccatcacc accatcctga ccgccgtgac 1560 cttctgcttc gccagcggcc agaacatcac cgaggaattc taccagagca cctgtagcgc 1620 cgtgtccaag ggctacctga gcgccctgcg gaccggctgg tacaccagcg tgatcaccat 1680 cgagctgagc aacatcaaaa agaacaagtg caacggcacc gacgccaaaa tcaagctgat 1740 caagcaggaa ctggacaagt acaagaacgc cgtgaccgag ctgcagctgc tgatgcagag 1800 cacccccgcc accaacaacc gggccagacg ggagctgccc cggttcatga actacaccct 1860 gaacaacgcc aaaaagacca acgtgaccct gagcaagaag cggaagcggc ggttcctggg 1920 cttcctgctg ggcgtgggca gcgccattgc tagcggagtg gctgtgtcta aggtgctgca 1980 cctggaaggc gaagtgaaca agatcaagtc cgccctgctg agcaccaaca aggccgtggt 2040 gtccctgagc aacggcgtgt ccgtgctgac cagcaaggtg ctggatctga agaactacat 2100 cgacaagcag ctgctgccca tcgtgaacaa gcagagctgc agcatcagca acatcgagac 2160 agtgatcgag ttccagcaga agaacaaccg gctgctggaa atcacccgcg agttcagcgt 2220 gaacgccggc gtgaccaccc ccgtgtccac ctacatgctg accaacagcg agctgctgag 2280 cctgatcaac gacatgccca tcaccaacga ccagaaaaag ctgatgagca acaacgtgca 2340 gatcgtgcgg cagcagagct actccatcat gtccatcatc aaagaagagg tgctggccta 2400 cgtggtgcag ctgcccctgt acggcgtgat cgacaccccc tgctggaagc tgcacaccag 2460 ccccctgtgc accaccaaca ccaaagaggg cagcaacatc tgcctgaccc ggaccgaccg 2520 gggctggtac tgcgataatg ccggcagcgt gtcattcttt ccacaagccg agacatgcaa 2580 ggtgcagagc aaccgggtgt tctgcgacac catgaacagc ctgaccctgc ccagcgaggt 2640 gaacctgtgc aacgtggaca tcttcaaccc taagtacgac tgcaagatca tgacctccaa 2700 gaccgacgtg tccagctccg tgatcacctc cctgggcgcc atcgtgtcct gctacggcaa 2760 gaccaagtgc accgccagca acaagaaccg gggcatcatc aagaccttca gcaacggctg 2820 cgactacgtg tccaacaagg gcgtggacac cgtgtccgtg ggcaacaccc tgtactacgt 2880 gaacaaacag gaaggcaaga gcctgtacgt gaagggcgag cccatcatca acttctacga 2940 ccccctggtg ttccccagcg acgagttcga cgccagcatc agccaggtca acgagaagat 3000 caaccagagc ctggccttca tcagaaagag cgacgagctg ctgcacaatg tgaatgccgt 3060 gaagtccacc accaatatca tgatcaccac aatcatcatc gtgatcatcg tcatcctgct 3120 gtccctgatc gccgtgggcc tgctgctgta ctgcaaggcc cggtccaccc ctgtgaccct 3180 gtccaaggac cagctgagcg gcatcaacaa tatcgccttc tccaacggac gcgtgaccga 3240 gctgctttac cggatgaagc gggctgagac atattgcccg agacccctgt tggcaatcca 3300 tcctactgag gctcgccaca aacagaaaat cgtggccccc gtcaaacaga cactcaattt 3360 tgacttgttg aaacttgcag gagatgttga gtcaaacccc gggcctatgg gcgtcaaggt 3420 cctgttcgct ctgatttgta tcgctgtcgc tgaagctaag ccaaccgaga ataatgaaga 3480 ctttaatatc gtggccgtgg cttctaactt cgctaccaca gacctggatg cagacagggg 3540 aaagctgcca ggcaagaaac tgcccctgga ggtcctgaag gagatggaag caaatgcccg 3600 gaaagccggg tgcacaagag gatgcctgat ttgtctgagc cacatcaagt gcactcctaa 3660 gatgaagaag ttcatccccg gccggtgcca tacctacgag ggcgataagg aatccgccca 3720 gggaggaatc ggagaggcta tcgtggatat tcccgaaatc cctggcttca aagacctgga 3780 gcccatggaa cagtttattg cacaggtgga tctgtgcgtc gactgtacta ccggatgcct 3840 gaagggactg gcaaacgtcc agtgtagcga cctgctgaag aaatggctgc ctcagcgatg 3900 tgctacattt gccagcaaga ttcagggcca ggtggacaag attaagggag caggaggcga 3960 ctgataattc tagacgagat ccgaacttgt ttattgcagc ttataatggt tacaaataaa 4020 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4080 tgtccaaact catcaatgta tcttatcatg tctgcgatcg cgatggcctg tgtttgagca 4140 taatgtactg accaggtgta acgttcatct gggtggtcgt agaggaatgt tcatgccata 4200 ccaatgcaat tttaatcatg tgaggatctt gatggagccg caagcgtttt ccagagtcag 4260 cttgactgga atctttgaca tgtgtgtgga agcatggaag atcttaagat atgatgatac 4320 caaatccaga tgccgcgcat gcgagtgcgg gggcaggcat gccaggttcc aacctgtatg 4380 tgtggaggtg accgaggagc tgagaccaga tcatttggtg ctgacctgca ctggtgcgga 4440 gttcggttcc agtggtgaag aaactgatta aagtgagtag tgggatgtta taaaagtgac 4500 cataaggtga tgtgagatgg acaaatttgg taatttttat gtatttttgt cttgcagcca 4560 tgagtgggag cgcttccttt gaagggggcg tctttagccc ttatctgacg gggcgtctgc 4620 ctcattgggc tggagtgcgt cagaatgtga tggggtctac agtggatgga agacctgttc 4680 agcctgctaa ttcttctact ctgacttatg ctactatgac ttcctcgcct ttggatgcag 4740 ctgcagctgc tgccgcttct gctgccgcca acactgttcg ggggatggcc ttggagatgg 4800 ggtattatgg aactgtagtg gccaacacca ctaccccaaa taaccccaca gccttgaatg 4860 aggacaagct gctagttctc atgtcccagc tggagtcttt gacccaacgc ctgggcgatc 4920 tagctcagca ggtgtcccag ctgaaggagc agactcaagc tgcaattacc actgcgaggg 4980 gaaattaaaa aaattcaaag aatcaataaa taaaccgaga ctttgttgat tttaaagtgt 5040 gtcattcttt atttaatttt tcgcgcgcga tatgccctgg accaccggtc tctatcattg 5100 aggacacggt ggatcttttc tagaacccga tagaggtggg attggatgtt gaggtacatg 5160 ggcataagac catctttggg gtgtagatag ctccactgca gagcctcatg ctccggggtg 5220 gtgttgtata taacccagtc atagcatggg cgttgggcat gatgttgcac aatatcttta 5280 aggaggagac taatggccac tgggagaccc ttggtgtaag tgtttacaaa tctattaagc 5340 tgggacgggt gcatccgagg tgagataatg tgcattttgg attggatttt tagattggca 5400 atgtttcccc ctagatctct cctgggattc atgttatgca agaccactag aacagtgtat 5460 ccggtgcact tagggaattt gtcatgaagt ttggagggga aagcatgaaa aaatttagac 5520 acacccttgt gtcctcccaa gttctccatg cactcatcca taataatggc aatgggccca 5580 tgggcggcgg cacgggcgaa cacgttcctg ggatctgaca catcatagtt gtggtcttgg 5640 gtcaggtcat cataagccat tttaataaac ttggggcgga gggtgccaga ttgggggatg 5700 aatgttccct cgggccccgg aacatagttt ccttcacata tttgcatttc ccaggctttt 5760 agttcagagg gggggatcat gtccacctgt ggagcgatga agaagacggt ctcgggggcg 5820 ggggtgatta agtgggagga cagcaagttc ctaagcagct gtgacttgcc acacccagtg 5880 ggaccgtaga tgacccctat aacaggttgc agatggtagt ttagggaaag acagctgccg 5940 tcctctcgca ggaggggggc gacctcgttc atcatttccc tcacatgcat gttttcccgc 6000 acaagttccg ataggaggcg ctctccaccc agggaaagga gttcttgaag agatgagaaa 6060 tttttcaagg gttttaagcc atcagccatg ggcattttgg agagggtttg ttgcaagagt 6120 tcaaggcggt cccagagttc ggtgatgtgt tctatggcat ctcgatccag catacttcct 6180 cgtttctggg gttgggacgg ctgcgggagt atggaaccag gcgatgggcg tccagcgctg 6240 ccagtgtccg gtccttccac ggtcgcagcg tccgagtcag ggtcgtttcc gtcacggtga 6300 aggggtgcgc gcctggctgg gcgcttgcga gggtgcgctt caggctcatc ctgctcgtgg 6360 agaaccgctg ccgttctgcg ccctgtgcat cggccaggta gcaattaacc atgagttcgt 6420 agttgagcgc ctctgccgcg tggcctttgg cgcgcagctt acctttggaa gtcttctgac 6480 aggtgggaca gtagagacac ttgagagcat agagttttgg ggctagaaag accgattctg 6540 gggagtatgc atcggcccca caggaggcgc agacggtttc gcattccacc agccatgtaa 6600 gatcgggctc gttggggtca aaaacaagtt ttccgccatg ttttttgatg cgtttcttac 6660 ctttgctttc catgagttcg tgcccccgtt gggtgacaaa gaggctgtcc gtgtccccgt 6720 agactgactt tatgggcctg tcctcgagcg gcgtgccgcg gtcctcttcg tagaggaact 6780 cggaccactc tgagacgaaa gcacgtgtcc aggccagcac aaaggaggct atatgggagg 6840 ggtagcgatc gttgtcaacc aaggggtcta ctttttccaa ggtgtgtaaa cacatgtccc 6900 cttcttccac atccaggaag gtgattggct tgtaagtgta tgccacgtga cctggggtcc 6960 cagacggggg ggtataaaag ggggcgggtc tctgctcgtc ctcactgtct tccggatcgc 7020 tgtccaggag cgccagctgt tgaggtaggt attccctctc gaaggcgggc ataacctccg 7080 cactcaggtt gtcagtttct aggaacgagg aggatttgat attgacagtg cctgccgaga 7140 tgcctttcat gagactgtcg tccatttggt cagaaaagac aatctttttg ttatcaagtt 7200 tggtggcgaa ggatccatac agggcattgg aaagcagttt ggcaatggag cgcatggttt 7260 ggtttttttc tttgtctgcg cgctctttgg cggctatgtt gagttggaca tattcgcggg 7320 ccagacattt ccattgtgga aatatggtag ttaattcatc tgggacgatt ctgactttcc 7380 agcctctgtt atgcagggta atcagatcca cactggttgc cacttctcct ctaagtggtt 7440 cattagtcca gcatagtcgc cccccttttc gagaacagaa agggggtagg ggatctagca 7500 tgagttcgtc tggggggtct gcatctatgg tgaaaatccc aggaaggaga tcttcgtcaa 7560 aatagctgat ggtggcgggg tcatccagag acatttgcca ttctcgagca gccagagcgc 7620 gctcgtaggg gttaagggga gtcccccatg gcatgggatg ggtgagtgca gaagcataca 7680 tgccacagat gtcatagaca tagagcggct cttccagaat ccctatgtaa gtgggataac 7740 atcgcccccc tctgatgctg gctcgcacat aatcatagag ttcatgtgag ggcgctagaa 7800 gacccgagcc caggttggtg cggttgggtt tttctgctct gtagaggatc tggcgaaaga 7860 tggcatggga gtttgatgag atggtgggtc tttggaagat gttgaaatgg gcatgaggca 7920 gtcccacaga gtcccttatg aagtgagcat aggagtcttg cagtttggcc accagctcgg 7980 cggtgaccag cacatccaaa gcacagtagt cgagggtctc tttgatgatg tcatagttag 8040 gttccccttt cttttcccac agctcgcggt tgagaaggta ttcttcgcga tccttccagt 8100 actcttcgag ggggaacccg tccttgtctg aacggtaaga acccagcatg taaaattgat 8160 tgacagcttt gtaggcacaa caccccttct ccacggggag tgagtatgct tgcgcggctt 8220 tgcgcagaga ggtgtgagta agggcgaaag tgtccctgac catgactttg aggaactgat 8280 gcttaaagtc tatgtcatcg caggccccct gctcccacag ttggaagtcc actcgctttt 8340 tgtaggcggg attgggcaaa gcgaaagtaa catcgttgaa taggatcttt ccagccctgg 8400 gcatgaagtt gcgagtaatg cgaaaaggct gaggcacttc tgccctgttg ttgataactt 8460 gggcagccaa gacgatctcg tcaaagccgt tgatgttgtg acccacaatg taaagttcta 8520 cgaagcgtgg gcgtcccttg atgtggggca gttttttaag ctcttcgtag gtcaagtcgt 8580 cagggtcagc gattccatat tgctccaaag cccagtcagg caggtgagga ttagcatgaa 8640 ggaaagaggt ccaaagatcc acggccagag ctgtttgtaa gcggtctctg tactgacgga 8700 aatgtcggcc taccgccatt ttttcaggag taacacagta aaaggtgcgc gggtcctttt 8760 cccagcgatc ccattgaagt tgcaaggcta ggtcgtgggc gaggttgacg agctgttcgt 8820 cccccgaaag tttcatgacc agcatgaaag ggacaagctg cttgccaaag gaccccatcc 8880 aggtgtaggt ttccacatcg taggtgagga agagcctttc tgtgcgagga tgagaaccga 8940 tcgggaagaa ctggatttcc tgccaccagt tggaggaatg gctgttgata tgatggaagt 9000 agaactccct acggcgcgcc gagcattcgt gcttgtgctt gtacagacgg ccacagtact 9060 cgcagcgctg cacgggatgc acctgatgaa tgagctgtac ctggcttcct ttgacaagaa 9120 atttcagtgg gaagttgagg cgtggcgtct gcatctcgtg ttgtattacg tcctggctat 9180 tggtctggcc atcttctgtc tcgatggtgg tcatgctgac gagcccgcgc gggaggcagg 9240 tccagacctc cgcgcggacg ggtctgagag cgaggacgag agcgcgcagg ccggaactgt 9300 ccagggtcct gagacgctgc ggagtcaggt cagtagggag agtacatagg tttacttgca 9360 taagtttttc cagggcatgt gggaggtcaa gatgatattt gatttctact ggcgagttgg 9420 tggagacatc gatggcttgc agggtcccgt gcccctgggg tgctaccacc gtcccttttt 9480 ttttcttgat cgggggcggt gttgcttctt gcatggtaag gtcgtcttct agaagcggcg 9540 gcgaggtcgc gcgccgggtg gcagtggcgg ttctggacct ggaggtagag gcggtagagg 9600 tacgtcggcg ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac ttgcgtgagc 9660 gacgacgcgg cggttgacgt cctggatctg acgcctctgg gtgaatgcta ccggacccgt 9720 gagcttgaac ctgaaagaga gttcaacaga atcaatttcg gtatcgttga cggctgcctg 9780 ccgcaggatt tcttgtacgt cgcccgagtt gtcttggtag gcgatctcgg ccatgaactg 9840 ctcgatctct tcttcttgga gatctccgcg gcccgctcgt tctacggtgg cagcaaggtc 9900 gttggagatg cgccccatga gctgtgagaa tgcattcatg cccgcctcgt tccagacgcg 9960 actgtagacc acggctccct cgggatctct ggcgcgcatg accacttggg cgaggtttag 10020 ttccacgtgt ctggtgaaga ccgcatagtt gcagagacgc tggaagaggt agttgagcgt 10080 ggtggcgatg tgctcggtga caaagaaata catgatccag cgacgaagcg gcatctcgct 10140 gatatcgccc agggcttcca accgttccat ggcttcgtaa aagtccacgg cgaagttgaa 10200 aaactgggag ttgcgagcgg acacggtcaa ctcctcctcc agaagacgga tgagctcggc 10260 gatggtggcg cgcacttcgc gctcaaaggc tcccgggatc tcttcctcct cttcttcttc 10320 caactcttcc tccactaaca tctcttctac ttcctcctca ggcggcgggg gtggaggagg 10380 gggcgcgcgg cgacgccggc gacgcacggg cagacgatcg atgaagcgtt cgatcacttc 10440 tccgcggcgg cgacgcatgg tctcggtgac ggcgcgcccg tcctccctgg gtcgcagagt 10500 gaagacgccg ccgcgcagct ccctgaaatg gtgactggga gggtccccgt ttggtaggga 10560 cagggcactg atgatgcatc ttattaattg ccctgtaggg actccgcgca aggacctgag 10620 cgtctcgaga tccacgggat ctgaaaatct ctgaacgaag gcttcgagcc agtcgcagtc 10680 gcaaggtagg ctgagcactg tttcttcggg gcgggctgct gagctagagg gttgtacgat 10740 gctgctggtg atgaagttaa aataggcagt tctgagacgg cggatggtgg cgaggagcac 10800 caggtctttg ggtccggctt gctggatgcg caggcggtcg gccattcccc atgcattatc 10860 ttggcacctg gccagatctt tatagtagtc ttgcatgagt cgctccacgg gcacttcttc 10920 ttcgcccgct ctgccgtgca tgcgtgtgag tccgtaccct ctctgtggtt ggacgagcgc 10980 caggtcggca acgacccttt cggctagaat ggcttgctgc acctgggtga gggtgttctg 11040 gaaatcatca aagtccacaa agcggtggta ggcccccgtg ttgatggtgt aggagcagtt 11100 ggacatgacc gaccagttga ctgtctggtg tcctggtcgt acgagttccg tgtacctgag 11160 ccgcgagtat gcgcgggagt cgaagatgta atcgttgcag gttcgcacca ggtactggta 11220 gccgatgagg aagtgaggcg gcggctggcg gtagagaggc catcgttcgg tggcgggcgc 11280 gccgggcgct aggtcttcta gcatgagacg gtggtatccg tagacgtacc tggacatcca 11340 ggtaataccg gcggcggtgg tggaggcgcg cggaaactct cgcacgcggt tccagatgtt 11400 gcgcagcggc atgaagtagt tcatggtggg cacggtctgg cccgtgaggc gcgcgcagtc 11460 attgatgctc tagatacggg caaaaacgaa agcgttgagc ggttcccttc cgtggcctgg 11520 aggaacgcga acgggttagg tcgcagcgta ccctggttcg agactaaaga aagcgagcaa 11580 ctcgaaccgg cagagtcgcg gctaacgggt attggcaatc ccgtctcgac ccaagccagc 11640 aaatccagga tacggatggg ggcccctttt gtttttcagg gcatgagtca ccggttaagg 11700 tttacaacgg ctgtttcatg cctttagaag tggctcgcgc ccgtagtctg gagaatcaat 11760 cgccagggtt gcgttgcggc gtgccccggt tcgagcctgc agcttgagtc ggccggtgac 11820 cgcggcaaac gagggcgtgg cggccccgtc gtttctaaga ccttgctagc cgacctctcc 11880 agtttacggg aacgagcccc cttttatttt ttttgttttt gccagatgca tcccgtactg 11940 cggcagatgc gcccacagcc cccacagcag cagcagcagg ctggcctacc ttctctacct 12000 cagccgctac ctgcaactac cgcggtggcc gctgtaagcg gggccggaca gcaggcggct 12060 cctcaatatg aattggactt ggaagagggc gagggattgg caagattggg ggcgccctcg 12120 cccgagcgcc acccgcgggt gcagatgaaa aaggacgttc gcgaatctta cgtgcccaag 12180 cagaatctgt tcagagacag aagcggcgag gagcccgagg agatgcgcgc gtcccgtttt 12240 aacgcgggtc gcgagctgcg acaaggactg gatcgaaaac gggtgttgag ggatgatgat 12300 tttgaggtgg atgaaatgac agggatcagc cccgctcgcg ctcacgtggc tgcagctaat 12360 ctggtgacag cttatgagca gaccgtgaag gaggaaagca acttccagaa atcattcaat 12420 aaccacgtgc gcaccctgat cgcacgcgag gaggtgaccc tgggcctgat gcacctgtgg 12480 gatctgctgg aagccatagt gcagaacccc actagcaaac ccctgactgc tcaactgttt 12540 ctggtggtgc agcacagcag ggataatgag gcattcagag aggcgctgct gaatatcact 12600 gaacctgagg ggagatggct gctggatctg gtgaatatcc tgcagagcat tgtagtgcag 12660 gaacgcagct tgcctttgtc cgagaaggtg gcggcgatca attactctgt gctgagtctg 12720 ggcaaatact atgccaggaa gatctacaaa accccttacg tgcccataga caaggaagtg 12780 aaaatagatg ggttttacat gcgcatgacc ctgaaagtgc taaccctgag cgatgacttg 12840 ggagtgtacc gcaacgacag gatgcaccgc gcggtgagcg ccagcaggag gcgcgagctg 12900 agcgacaaag aattaatgca cagcttgcaa cgagccctga cgggagccgg gacggagggg 12960 gagaactact ttgacatggg tgcagacttg cattggctgc ctagtcgcag ggcattggaa 13020 gcggcaggcg atgggcccta tgtagaggaa gtagtagacg aggacgatga ggagggcgag 13080 tacctggaag actgatggcg cgacccgtat ttttgctaga tggaacaggc gccggaccct 13140 gcgatgcggg cggcgctgca gagccagccg tccggcatta attcctcgga cgattggacc 13200 caggccatgc aacgcatcat ggcgctgacg acccgcaacc ccgaagcctt tagacagcag 13260 cctcaggcca accgcctttc ggccatcctg gaggccgtgg tgccctctcg ctccaacccc 13320 acccacgaga aggttctggc catcgtgaat gccctggtgg agaacaaggc catccgctcc 13380 gatgaagccg ggctggtata caacgccttg ctcgagcgcg tggctcgcta caacagcagc 13440 aatgtccaga ctaacctgga caggatggtg accgacgtgc gcgaggccgt gtcccagcgc 13500 gaacggttcc atcgcgagtc taacctgggt tccatggtag cgctgaacgc tttcctcagt 13560 tcccagcctg ccaatgtgcc ccggggacag gaagactata ccaactttat tagcgccctg 13620 agactcatgg tagccgaggt tcctcagagc gaggtgtacc agtccggtcc agactacttt 13680 ttccagacaa gcaggaacgg tatgcagaca gtgaacttaa gccaggcttt caagaacctg 13740 caagggctgt ggggagtcca agctccagtg ggcgacaggg cgaccgtgtc gagcctgttg 13800 actccaaatt cccgtttgct gctgctgctg gtgtccccct tcactgacag cggcagcata 13860 aacagaaact cctacttggg ctacctgata aacttgtatc gcgaagctat aggtcaggcc 13920 cacgtggacg aacagaccta tcaggagatc actaatgtga gtcgcgctct gggccaggac 13980 gaccctggaa acctggaagc tactctaaac tttctgctga ccaaccgctc gcaaaaaatc 14040 cctcctcagt atacattaac tgcggaggag gaacggatct tgagatacgt gcagcagagc 14100 gtgggtctgt tcctgatgca agagggtgcg acccctagcg ccgcgcttga tatgacagcg 14160 cgcaacatgg agcccagcat gtatgccagc aacagaccat tcattaataa attgatggat 14220 tacttccatc gcgcggccgc tatgaactct gattacttca ccaatgctat tctgaacccc 14280 cattggctgc ctccgcctgg tttttatact ggcgagtatg acatgcctga ccccaacgat 14340 gggttcttgt gggacgatgt ggacagcgtg gcgttctcgc ctaccgctcc tcgtactttt 14400 tggaagaagg aaggtagtga cagaagaccc tcctccgtgc tgtcaggacg tgagggtgct 14460 gccgcggcgg tccccgatgc tgcaagcccc tttcccagtc tgccattttc actaaacagc 14520 gtgcgcagta gcgagctggg gagaataacc cgccctcgct tgctgggcga ggacgagtat 14580 ttgaatgact ccctactgag acccgagcgg gaaaagaact tccctaataa tgggattgaa 14640 agcctggtgg ataagatgag cagatggaag acctatgccc aggagcacag agatgagcct 14700 agaatcttgg gtcctacagt aggcacccgc agacgccagc gccatgatag acagcggggt 14760 ctggtgtggg acgatgagga ttctgcagat gacagcagcg tgttggactt gggcgggagg 14820 ggaggtgtgg gcaacccgtt cgcacacttg cgtccccgta ttggacgcat gatgtaaaag 14880 tgaaaataaa aaaggaactc accaaggcca tggcgaccag cgtgcgttcg ttctttctgt 14940 tgttgtatct agtatgatga ggcgcaccgt gctaggcgga tcggtggcgt atccggaggg 15000 tcctcctcct tcgtacgaaa gcgtgatgca gcaggtggcg gcggcggcga tgcaaccccc 15060 cttggaggct ccttacgtgc ccccgcggta cctggcacct accgagggga gaaacagcat 15120 tcgttattcg gaactcacac ccttgtatga caccacccgg ttgtacctgg tggacaacaa 15180 atcggcggac attgcctcgt tgaactatca gaacgaccac agcaacttct tgacaacggt 15240 ggtgcagaac aatgacttta cccccacgga ggccagcacc cagaccatca actttgacga 15300 gcgctcccgg tggggcggtc agctgaagac catcatgcac accaacatgc ccaacgtgaa 15360 cgagttcatg tttagcaaca agttcagggc tagggtgatg gtgtccagaa ccacacctaa 15420 agaggtgaca gtcacaacag actatgatgg tagtcaggac atcttggaat acgagtgggt 15480 tgactttgag ttaccagaag gcaacttctc tgccaccatg accatagacc tgatgaataa 15540 tgcaattgtt gataattacc taaaagtggg tagacagaat ggggtactgg agagtgacat 15600 aggtgttaag tttgacacta ggaactttag gcttggttgg gacccagtga cagagttggt 15660 catgcctggg gtctacacca atgaagcttt ccatcctgac atagtcctac tacctggctg 15720 cggagtggac ttcactgaga gccgcctcag taatctgcta ggcattagaa agaaacagcc 15780 attccaggaa gggttccaga tcatgtatga ggatctggag ggtggtaaca tccccgccct 15840 gcttgatgta aatgcatatg agaagagcaa ggaagataat acaaccacca caaatgaagc 15900 tgtggccgcg gcttcatcta ctgaagccaa agctgtggta gatgcttcca cttcaacaga 15960 aaacaccact gatgaaaaag tcaccagggg agatacattt gccacccctg aacaagagaa 16020 ggcagctgag gcagagtctg atattatgct tctgtccacc gatgaaaacg aaactaaaaa 16080 acaactggtt attcgagcgg tgaccaagga tagtaaggac aggagttata atgtattgtc 16140 agatggaaag aacacagctt accgtagttg gtacctggca tacaattatg gcgaccgtga 16200 gaaaggggtg cgttcttgga cactgcttac cacctcggat gtcacctgcg gcgtggagca 16260 agtctattgg tcgctaccag atatgatgca agatccagtc acctttcgct ccacacgcca 16320 agttagcaac tacccagtgg tgggcgcaga gctgctccca gtgcattcca gaagcttcta 16380 caacgagcaa gccgtctact cgcaacagct ccgccagtac acctcgctca cgcacgtctt 16440 caaccgcttc cccgagaatc agatcctcgt ccgcccgccc gcgccaacca ttaccaccgt 16500 cagtgaaaac gttcctgctc tcacagatca cgggaccctg ccgctgcgca gcagtatccg 16560 gggagtccag cgcgtgaccg ttactgacgc cagacgccgc acctgtccat acgtatacaa 16620 ggccctgggc atagtcgcgc cgcgcgtcct ttcaagccgc actttctaaa aaaatgtcca 16680 ttctcatctc gcccagtaat aacaccggtt ggggcctgcg cacacctagc aagatgtatg 16740 gaggcgctcg cagacgctcc actcagcacc ctgtgcgcgt gcgcgggcat ttccgcgctc 16800 cctggggcgc cctcaaggga cgctctcgta ctaggaccac cgttgacgat gtgatcgacc 16860 aggtggtcgc cgatgcacgt aactataccc ccgcagccgc acctgcatcc accgtggatg 16920 cggtcattga cagcgtggta gccgatgcgc gcgcctatgc tcgcgccaag agcaggaggc 16980 ggcgtattgc caggcgtcac cgagctactc cagccatgcg agctgcaaga gctttattgc 17040 ggagagccag acgtgtgggg cgaagagcca tgcgtagagc ggccagacgc gcggcttcag 17100 gtgccagcgc aggcagggtc cgcaggcgcg cggctacggc ggcagcggcg gccatcgcta 17160 gcatgaccaa accacgaaga ggcaatgtgt attgggtgcg cgacgccgcc accggccagc 17220 gcgtgcccgt gcgcacacgc ccccctcgca cttagaagat actgagcagt ctccgatgtt 17280 gtgtcccagc ggcgagatgt ccaagcgcaa attcaaggaa gagatgctcc aggtcatcgc 17340 gcctgagatc tacggtcctg cggtgaagga tgaaaaaaag ccccgcaaga tcaagcgggt 17400 caaaaaggac aaaaaggaag aagatggtga tgatgggctg gtggagtttg tgcgcgagtt 17460 tgccccaagg aggcgcgtgc agtggcgcgg gcgcaaagtg tggccggtgt tgagaccggg 17520 gaccacagtg gtctttacgc caggcgagcg ctccagcacc gtttccaaac gctcttatga 17580 tgaggtgtac ggggacgatg atattctcga gcaggcggct gatcgccttg gcgagtttgc 17640 atatggcaaa cgcagccgct cgggagccaa ggaagaggca ttgaccatcc ccttggatca 17700 tggaaatccc accccaagcc tcaaacccgt gaccctgcaa caagtgctgc ccacgccgcc 17760 acgcaagggc atcaagcgcg agggcgagga tctgtatccc accatgcagc tgatggtgcc 17820 caagcgccag aagctggaag acgtgctgga gaaaatgaaa gtggatcctg aaatccagcc 17880 tgaagtcaaa gtgaggccaa tcaagcaggt ggcgcccggt ttgggggtac aaaccgtgga 17940 tatcaagatc cccaccgagt ccatggaaat tcaaaccgaa cccatgaagc ccacctccag 18000 caccattgag gtgcagacgg atccttggat gcccgcgcct gctcctgtta ccactactac 18060 tcgaagacct agaagaaagt atggttcagc caacctgata atgccaaact atgctctgca 18120 tccatcaatc atacccactc ctggctaccg cggcactcgc tactaccgca gtcacagcac 18180 ccgccgacgt aaagcacctg ccacccgccg ccgtcgccgc cgccgtgcca ctagcaaact 18240 taccccctcg gctatggtgc ggagagtgta ccgtgatggg cgcgcagctc ctctgacact 18300 gccgcgcgcg cgctaccatc ctagcattgc catttaacaa ctctgcctcc ttgcagatat 18360 ggccctcact tgccgccttc gtattcctat tgctggctac cgcggaagaa agtcgcgccg 18420 tagaagagca gggttgtctg ggagcgggat gcgtcgccac cggcggcggc gcgccatcag 18480 caaacggttg gggggtggat ttcttcccgc tttgattccc atcatcgccg cggcgatcgg 18540 cgcgatacca ggcatagctt ccgtggcggt gcaggcctcg cagcgccact gacattggaa 18600 aaagatatct tataaataaa aatagaatgg actctgacgc tcctggtcct gtgatatgtt 18660 tttgtagacg agatggaaga catcaatttt tcatccctgg ctccgcgaca cggcacgcgg 18720 ccgtatatgg gcacctggag cgacatcggc aacagccaac tgaacggggg agccttcaat 18780 tggagcagtc tatggagcgg gcttaaaaat tttgggtcca ctataaagac ttatgggaac 18840 aaagcttgga acagcagcac agggcatgcg ctgagacaaa agcttaaaga tcagaatttc 18900 caacagaagg tggtcgatgg tatcgcctct ggaatcaatg gggtggtaga tctggccaac 18960 caggccgtgc agaaacagat taacagtcgc ctggacccgg ctcccccagc tcctattcat 19020 gagttaatgc aagtggagga agagctccct tcattggaaa agcggggcga taagcgacct 19080 cgtccagata tggaggaaac gctgctgacc aaggtggatg agccgccctc ctatgaagag 19140 gctgtaaaac tgggaatgcc cactacaaag cccattatgc ctctggccac tggagtgatg 19200 aagccatctc agtctaaacc tgcagttgct gctacattgg acttgcccgc tcccgtggcc 19260 acccccaaac ctgtcgccgc cccgaagccc accgccgtgc aacccgtggc cgtggccaga 19320 ccgcgtcccg gtggtcggcc gaatgcaaac tggcagagca ctctgaacag catcgtgggt 19380 ttgggagtgc acagtgtgaa gcgccgtcgc tgctattgat taaatatgga gtagcgctta 19440 acttgcttgt ctgtgtgtgt atatgtcgat gccgcccgcc gtgctacagc aaagagagaa 19500 ggagaagagg cgccgctgag ttcctttcaa gatggccacc ccatcgatgc tgccccagtg 19560 ggcgtacatg cacatcgccg gacaggacgc ttcggagtac ctgagtccgg gtctggtgca 19620 gttcgcccgc gccacagata cctacttcaa tctggggaac aagtttagga accctaccgt 19680 ggctcccacc cacgatgtga ccaccgaccg tagccagcgc ctgacgctgc gctttgtgcc 19740 cgttgaccgg gaggacaata cctactccta caaagtcaga tacaccctgg ctgtgggaga 19800 caacagggtg ttggatatgg ccagcaccta ctttgacatc aggggcgtgt tggacagagg 19860 acctagcttc aaaccatact ctggcactgc ctacaactcc ctggctccaa aaggagctcc 19920 aaactccagt cagtggcaac aaaaggaaaa caatggtcaa ggtgatgcaa agactcacac 19980 ctatggtgta gctgccactg gaggtattga cattgacaaa aatggtcttc aaattggaat 20040 cgatgaaact aaagaagata ataacgaaat ttatgcagac aaaacattcc aacctgaacc 20100 tcaaattgga gaagaaaact ggcaagatag cgaaaactat tatggaggca gggctcttaa 20160 accggaaacc aagatgaagc cttgctatgg ttccttcgct agaccaacta atgcaaaggg 20220 aggtcaagcc aaaattaaac cagctcaaga gggtcaacag tctatagatt atgacataga 20280 cctggctttc tttgatattc caagcactgg cggaggcaat ggcacaaatg taaatgacaa 20340 gccagatatg gttatgtata ctgaaaatgt aaatctggaa actccagaca ctcatcttgt 20400 ttacaagcca ggaacttcag atgacagttc cgaggccaat ttaactcagc aagccatggc 20460 taacagaccc aactatattg ggtttagaga taactttatt ggcgtcatgt actacaacag 20520 cactggcaac atgggagtgc ttgctggtca agcatcccag ctaaatgctg tggtggacct 20580 gcaagacaga aacaccgagc tgtcttatca gctattactt gactctctgg gcgacagaac 20640 caggtatttt agtatgtgga atcaggcggt ggacagctat gatcctgatg tgcgcattat 20700 tgaaaaccat ggtgtggaag atgaattgcc aaactattgc ttcccattgg acggagctgg 20760 cactaatgct gtttaccaag gagttaagac aaaagaggat aataatggcg aatgggaaac 20820 agacacaaat gttgcatcgc agaatcagat atgcaagggc aacatatatg ctatggagat 20880 caacctgcaa gccaacctgt ggaaaagttt cctttactcc aacgtggctc tgtacctacc 20940 agactcctac aagtacactc catccaacgt gacactccct accaacacta acacctatga 21000 ctacatgaat ggcagggtgg tgtctccatc cctggtggat gcctacatta acattggcgc 21060 caggtggtct ctggatgcca tggacaatgt caaccctttc aaccaccacc gcaatgccgg 21120 cctgcgctac cggtccatgc ttctgggcaa cggccgatac gtgcccttcc acatccaagt 21180 gccccagaaa ttcttcgcta tcaagaacct gctgcttctc ccaggctcat acacctacga 21240 gtggaacttc cgcaaggatg tcaacatgat cctgcagagt tcccttggca atgacctcag 21300 aaccgatggg gccaccatcc agtacaccag catcaatctc tatgccacct tcttccccat 21360 ggctcacaac actgcctcca ccctggaagc catgctgcgc aatgacacca atgaccagtc 21420 cttcaatgac tacctctcag ctgccaacat gctttacccc atccctgcca atgccaccaa 21480 cgtgcccatc tccatcccat ctcgtaactg ggctgccttc aggggctggt ctttcacccg 21540 cctcaagacc aaggagaccc catctctggg atcagggttc gatccctact tcgtctactc 21600 aggctccatt ccatacctgg atggaacttt ctaccttaac cacactttca agaaagtctc 21660 catcatgttt gactcttctg tcagctggcc aggcaatgac aggctgctga ctcccaatga 21720 gttcgaaatc aagcgcactg ttgatgggga agggtacaat gtggcacaat gcaacatgac 21780 caaagactgg ttcctggttc agatgctctc ccactacaac attggctacc agggcttcta 21840 catcccagaa ggatacaagg accgcatgta ctccttcttc agaaacttcc agcccatgag 21900 ccgccaggtg gtcgatcagg tcaactacaa agactacatg gcagtcaccc ttgcctatca 21960 gcacaacaac tctggctttg tgggctacct cgcgcccacc atgcgacagg gccaacccta 22020 ccctgctaac tacccatacc cgctcattgg caagactgca gtcaacagtg tcacccagaa 22080 aaagttcctc tgcgacaggg tcatgtggcg catccccttc tccagcaact tcatgtccat 22140 gggggccctt accgacctgg ggcaaaacat gctttatgcc aactccgccc acgcgctaga 22200 catgaatttc gaagtagacc ccatggatga gtccaccctt ctctatgttg tcttcgaagt 22260 cttcgacgtg gtcagagtgc accagcccca ccgcggcgtc atcgaagctg tctacctgcg 22320 cacccccttc tcagctggta acgccaccac ataagcgcct tgcttcttgc aagtggctgc 22380 agcagcatgg cctgtggatc ctccactgga tccaatgagc aagagctcag ggccatcgcc 22440 atagacctgg gctgtggacc ctatttcctg ggaacctttg acaagcggtt tccaggcttc 22500 atggctcctg acaagctcgc ctgtgccatt gtcaacacgg cagggcgcga gactggtggt 22560 gagcactggc tggcttttgg atggaacccc cgctccaata cctgctatct ctttgacccg 22620 tttgggtttt cagacgagcg cctcaagcag atctatcaat tcgagtacga ggggctcctg 22680 cgccgcagtg ccctggctac taaggaccga tgcatcactc tggaaaagtc tacccagacc 22740 gtgcagggtc cgcgctcggc tgcctgcggg ctcttctgct gcatgttcct ccatgctttt 22800 gtgcactggc ccgaccgccc catggacaac aaccccacca tgaatttgct gacgggggta 22860 cccaacaaca tgctccaatc gccccaagta gagcccaccc tgcgccacaa ccaggaggca 22920 ctctatcgct tcctgaactc ccactcatct tactttcgtt ctaaccgcgc gcgcattgag 22980 aaggccactg ccttcgatcg aatgaataat aacatgtaaa ccaaattgtg tgtggctcaa 23040 ataaacagca ctttattgtt tacatgcact gaggctctgg gatgatcatt ttttaaaaat 23100 cgaaggggtt ctggcgggaa tcagcatggc cagatggcag ggacacgttg cggaactgga 23160 acttgttctg ccacttgaac tcgggaatca ccagcctggg aactggaatc tctggaaagg 23220 tatcttgcca tagctttctg gtcagttgca gagcgccaag caggtcagga gcagatatct 23280 tgaaatcaca gttggggcca gaattctggg cgcgggagtt gcggtacact gggttgcagc 23340 actggaacac cataagggca gggtgtctca cgctcgccag cacggtctcg tcactgatgc 23400 aagacacatc caggtcttca gcattggcca ttccaaaggg ggtcatcttg caggtctgtc 23460 tgcccatcac gggagcgcag ccaggtttgt ggttgcaatc acaatgaagg gggatcagca 23520 tcatcttggc ctggtcgggg gtaatccctg ggtaaacagc cttcatgaag gcttcatact 23580 gcttgaaagc ttcctgggct ttggttccct cggtgtagaa cactccacaa gacttgctgg 23640 aaaactgatt agtagcgcag ttggcatcat tcacacagca gcgggcgtcg ttattagcca 23700 gctggaccac attcctgccc cagcggttct gggtgatctt ggctcgatct gggttctcct 23760 tcaacgcgcg ctggccgttc tcgctcgcca catccatctc aatgacatgt tccttctgga 23820 tcatgatgtt gccatgcagg catctaatct tgccttcata atcagtgcag ccatgaggcc 23880 acagcgcgca cccggtgcac tcccaattgt tatgggggat ctgggaatgg ctatgaacca 23940 gcccttgcag gaatcttccc atcatcacag ccagggtctt tatgctggta aaggtcagcg 24000 ggataccgcg gtgctcctcg ttcacatact gctggcagat gcgtctgtag tgctcggcct 24060 gctcgggcat cagcttgaaa gaggttttca actcattatc cagcctgtat ctctccatca 24120 tgatggacat tacttccatg cccttctccc aggcagaaac aatagggaga ctcaggggat 24180 tcttgacagt agagacaacc ttacttaagg ggtcatcact gccaatcttt tcgatgcttc 24240 tcttgccatc cttctcggtg atgcgcaccg gcgggtagct gaatcccaca gccaccaact 24300 gagcctcttc cctttcgtct tcgctgtctt gactgatgtc ttgcagagga acatgtttgg 24360 ttttcctggg tttcttcttg ggcggcagct ctggaggact ctggctccgt tccggagacc 24420 ccatggatga gcgagagttg tcgctcacca cttggatctg gctgcctgta gaagaactgg 24480 accccacgcg gcggtaggtg ttcctcttgg taggcagagg tggaggcgac gggctccggt 24540 ccggtctggg tggcggatgg ctggcggagc cccttccgcg ttcgggggtg cgctccagat 24600 ggcggtcgtc tgactgacct ccgcggctgg ccattgtgtt ctcctaggta gagaaacaag 24660 acatggagac tcagccatcg ctgccatcgc catccaccac cacaagcacc gccgaggagg 24720 aggagtgttt aaccacccca ccatgcagcc ccgctaccac caccagcacc cttgaaagcg 24780 aggtcgacac ggtcgtggag gatttacagg ctatggaaga tattgaggca gctgtcgagc 24840 aagaccccgg ctatgtgaca ccggcggagc atgatgagga tctagcgcgc tttctcgacg 24900 gtgtggagaa agcgaaacaa gatgaggacg aggaagaggc agaagcacaa ccatcggtgg 24960 ccgactacct caccggccta gggctagaag acgtgctgct taagcatctt gcaaggcaga 25020 cagtcatagt caaagacgcc ctgctagagc gctccgaggt gccactcagt gtggaagacc 25080 tcagtcgcgc ctatgagcta aacctcttct cgcctcgcaa gccccccaag cgtcagccca 25140 acgggacctg tgagcccaat ccgcgcctca acttctatcc agccttcact gtgcccgaag 25200 tactagctac ctaccacatc tttttcaaga accaaaagat ccccatctcc tgccgcgcca 25260 atcgcacccg cgcagatgcc ctactcaact tggggcccgg cgctcgcata cctgatatcg 25320 cttccttgga agaggttcct aagatctttg agggtctggg caatgaggaa actcgggcag 25380 caaacgctct gcaaagagaa acagatgatg gtgaacacca cagcgctctg gtggagctcc 25440 agggcgacaa cgctcgtctt gcagtcctca aacgcagcat cgaggtcacc catttcgcct 25500 accccgcact taatctccca cccaaagtca tgagctcggt catggacacg ttgctcatga 25560 agcgcgcgag ccccatctcc gaggatcaga acatgcagga ccccgatgcc tcagatgaag 25620 gcaagcctgt agtcagcgac gagcaactgg ctcgctggct aggctctgac tccccccagt 25680 ctttggagga gcggcgcaag cttatgatgg cagtggtcct gatcacagcg gagctggagt 25740 gtctccgccg cttcttcact gacccagaga ccctgcgcaa gcttgaggag aacctgcatt 25800 acacattcag tcatgggttc gtgcgccagg cgtgcaagat ctccaacgtt caactcacca 25860 acctggtctc ctacctgggc atcttgcatg aaaaccggct ggggcagaac gtgctccaca 25920 ccaccctgaa gggggaggcc cgccgcgact atatccgcga ctgtatctac ctctacctat 25980 gctacacctg gcaaagcggg atgggtgtgt ggcaacagtg cttggaagag caaaatctaa 26040 aagagctgga aaagctgctt cagaaatctc ttaaatctct gtggaccggg ttcgatgagc 26100 ggaccaccgc ttcggacatg gccgatatta tcttccccga gcggctcaga cacactctgc 26160 gcgacgggct gcctgacttt gccagccaga gcatgctaca aaactttagg tcattcatct 26220 tggaacgctc cgggatcctg cccgccactt gctgcgcact gccctccgat tttgtgccca 26280 tcacctaccg ggagtgcccc ccgccgctat ggagccactg ctacctgttc cgcctggcca 26340 actacttggc ctaccactct gatgtgatag aagatgttag tggcgaaggg ctcctggagt 26400 gccactgccg ctgcaacctc tgcacccccc accgctccct cgcctgcaat ccccagctgc 26460 tgagcgaaac ccagatcatc ggcaccttcg agttgcaagg tcccagcggc gaaggcgagg 26520 ggtcctctcc ggggcaaagt ctgaaactga ctccggggct atggacctcc gcttaccttc 26580 gcaagttcgc ccccaaagac taccacccct atgagatcag gttttatgaa gaccaatcac 26640 agccccccaa ggccgaactg acggcctgcg tcatcaccca gggggcaatc ttggcccaat 26700 tgcaagccat ccaaaaatcc cgccaagaat ttttgctgaa aaagggacac gggatctatc 26760 tagaccccca gaccggtgag gagctgaata cacgcttccc tcaggatgcc ccgaggaggc 26820 aagagaatga aagttcagat gccgcccgag gaggagctgg aagactggga cagtcaggca 26880 gaggaggaag actgggacag ccaggcagaa gaggaggaca gcctggagga ggacagtctg 26940 gaggaaggcg aggagcccaa ggaagaggca gccgccgcca gaccatcgtc ctcggcggtg 27000 gagacaagca aggtcccaga cagcacggct accacctccg ctccagctca aggggccgct 27060 cggcgaccca acagtagatg ggacgagacg ggtcgcttcc agaaccccac caccgtcaag 27120 accggtaagc aggagcggca gggatacaag tcctggcggg ggcataaaag tgccatcatc 27180 gcttgcttgc aggagtgtgg gggcaatata tcctttgcca gacgctacct gctattccat 27240 cacggggtga atttcccccg caacatcttg cattactacc gtcacctcca cagcccctac 27300 taccagcagc aagagacagc agaggaaacc agcggcaact ccgagagtta gaaaaccagc 27360 agctaaaaaa tccacagcgg cggcagcagg tgcaggcgga ctgaggatca ccgcgaacga 27420 gccagctcag accagggagt tgaggaatcg gatctttccc accctctatg ccatattcca 27480 acaaagtcgg ggtcaggaac aagaactgaa agtaaaaaac agatctcttc gctcgctcac 27540 ccgcagttgt ttgtatcaca agagcgaaga ccaacttcag cgcactctcg aggacgccga 27600 ggctctcttc aacaagtact gcgcgctgac tcttaaagag tagactgcgc gcgcttggcg 27660 agaaaaggcg ggaattacgt cacctcttgg ccacacctgt gcttcattat gagtaaagaa 27720 attcccacgc cttacatgtg gagctatcag ccccagatgg gattggccgc tggcgccgcc 27780 caggactact ccacccgcat gaattggctc agcgccggtc ccgcgatgat ctcacgggtt 27840 aatggtgtga gagagcaccg aaaccagata ctcctagaac agtccgccct caccgccact 27900 ccccgcaatc acctcaaccc ccgtaattgg cccgccgccc tggtgtacca ggaaactcct 27960 gctcccacta cagtactact tcctcgtgac gcccaggccg aagttcagat gactaactca 28020 ggtgtacagc tggcgggtgg tgccaccctg tgtcgtcacc ggccaagacc gggtataaag 28080 ggcctggtga tcagaggccg aggtattcag ctcaacgacg agtcggtgaa ctcttcgctt 28140 ggtctgcgac cagacggcat cttccaaata gctggttgtg ggagatcttc cttcactcct 28200 cgtcaggctg tcctgacttt ggagagttcg tcctcgcagc cccgctcggg cggcatcggg 28260 actctccagt tcgtggagga gtttactccc tcggtctact tcaacccctt ctccggttct 28320 cctgggcttt acccggacga gttcatcccg aactacgacg ccatcagtga agcggtcgac 28380 ggctacgatt aatgtctaat ggtggcgcgg ctgagctagc tcgactgcga cacctagacc 28440 actgccggcg ctttcgctgc tttgctcggg atctctgcga gttcatctac ttcgagtacc 28500 ctgacgaaca tcctcaggga cctgcccacg gagttcggat taccattgaa ggggctatcg 28560 attctcacct gcttcggatc ttcaccgctc ggccagtgct agttgagcgc aaccagggcg 28620 acaccaccat ctccctctgc tgcatttgtg acaaccccgg attgcatgaa agcttttgtt 28680 gtcttctttg tactgagtat aataaaagct gaaattagag actactccgg actctcttgt 28740 cgtctgaaca acaccaacca gacccttcac ttcagcggga accagactac tcttcactgt 28800 aaggcttata actataagta tcttacttgg atatacaaag gaacaccgtt tgctgtggta 28860 aacaggtgct ccaacgacgg tgttctcctc accttcctag gcaacttctc caactttacc 28920 ttttctgttc gcagaaacaa gcttaccctc cttcagccct actttcctgg gatctatacc 28980 tgcctcagtg gaccttgcaa ccacactttt cacctgattg aaaactctac ccttaccttc 29040 ccagcgccaa tccctactaa cagctcggag tccaactctt ccattaccgc tgatactaac 29100 actcctaaaa ccggaggtga gctccgcagc cttcccccgg ctgcagataa cccttgggtg 29160 gtagcgggat ttgtagcgct aggaatagtt gcgggtgggc tcgcgttcgt cctctgctac 29220 ctatacctta cctgctgctc atatttagta gtactgtgct gttggtttag aaaatggggg 29280 cgctactaat cacacttgct ttactttcgc ttttgggtct gagctcggct aatagcgaga 29340 aaccaagctg tctagaaaca aactctccag actgtgtggt tcctcatggg ctctcagacc 29400 cagctgatga tccatgctta acttttgacc cagaaaaaaa ctgctcggtg actatgcagc 29460 cctatgctta catgtgcaca tctgttataa agtgcggatg gggctgtaaa ccgattgaaa 29520 ttacccacaa aggcaaaacc tggaataata gtttgtttaa cacatggcag cctggagacg 29580 agcagtggta tacggccggc cactggtgga gatgactgac cccatggaaa actcctctgc 29640 caacgacctg gacatggacg gccgttcatc tgagcagcga ctggtccaga tgcgcattcg 29700 ccagaagcag gaacgcgccg ccagagagct caaggatgcc attgaaattc acctgtgcaa 29760 gaagggcatc ttttgcttgg ttaagcaagc aaagatttct tatgaaatca ctgacaacga 29820 ccaccgcctg tattatgagc tcggtccaca gcggcagaaa ttcacctgca tggttggagt 29880 caaccccata gtcatcactc agcaggctgc agaaattaaa gggtgcatcc actgttcctg 29940 tgattcccaa gaatgcgtcc acaccatagt caagaccctc tgcggccttc gagatcttct 30000 tccaatgaac taaccccttc ccccaaccca ataaaacatt ggttttaatc ataataaaaa 30060 atcacttact ttaaatctga aacagtgtct ccgtccaagt tttcttgtag caccacttca 30120 ctcccctctt cccagctctg gtactgcaag ccccggtggg ctgcaaactt tctccacacc 30180 ttaaaaggga tgtcaaattc ctcttgtcca acaatcttca ttgtctcttc ctagatgtcc 30240 acaaagcgcg cgcgggtgga agatgacttt gaccctgtct acccatacga tgctgagctg 30300 gcaccgtctg tacccttcat cgcccctccc ttcgtttcgt cagacggatt tcaagaaaaa 30360 cccctgggag ttctgtccct aagactagcc aacccagtca ctactaaaaa tggggaactc 30420 acacttaaac tgggagatgg ggtgggcata gactcagatg gaaacctcac agcacagaca 30480 gttactaaag caacatcccc ccttactgtt tccaataacg caattgcact taacatggac 30540 aaaccttttt acagtagcaa tggaaaacta tccttacaag ttacatcacc attaaagata 30600 gtcgactctt taaatacatt ggctattggc tatgggcaag gcttaggact aaacaatagt 30660 gctcttgctg tgcaattagc atctcccctt acatttgaca gcaacagcaa aattaaaata 30720 aatttgggaa gcgggccatt aaaaattaat gcgaataaac tgtcaattaa ctgcctaagg 30780 ggtgtatatg taacaactga cggaacttcc attgaaacaa atataagctg ggcaaaagga 30840 atgaggtttg aaggtaatgc catggctgta aacgttgaca gcaccaaagg tctacaattt 30900 ggcactacca gcacagaatc aggagtcact aacgctttcc ctatccagtt aaagattgga 30960 tctggtctta gttttgacag cacaggagca cttgtagctt gggataagga taatgacaag 31020 cttacactgt ggacaaccgc tgacccatca cctaattgta ccatatatac agacaaggat 31080 gctaaactta cactttgtct tacaaaatgt ggcagtcaaa tactaggcag tgtttcagta 31140 ctggctgtta aagctggaac cctacagcca atcagtgaaa aaataggtac tgctttggtt 31200 tcactaaaat ttaataacaa cggtgtattg ttaagcaact ccacattaag taatgaatac 31260 tggaactaca ggaagggaga tgtcacacca gccgaagcct atactaatgc tgtgggtttt 31320 atgccaaaca tcaaggcata tcctaaaaac acaaactctg cctcaaaaag ccacattgta 31380 ggacaagtgt accttaatgg agatgaaact aaaccaatgc atttaatcat tacatttaat 31440 gaaaccagtg atgaaacatg cacatattcc ataacgttcc aatggaaatg gaacattgga 31500 acatacacca gcgacaccct tgcaacaagc tcctttacct tttcttacat tgcccaagaa 31560 taaaaactgc agacaacaat aaagtttaaa tgttttattt aaacagtttc acagaaccct 31620 agtattcaac ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct 31680 ggccttaaaa agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac 31740 ggtttcctgt cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact 31800 taagttcatg tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt 31860 aacgggcggc gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag 31920 gatagggcgg tggtgctgca gcagcgcgcg aataaactgc tgccgccgcc gctccgtcct 31980 gcaggaatac aacatggcag tggtctcctc agcgatgatt cgcaccgccc gcagcataag 32040 gcgccttgtc ctccgggcac agcagcgcac cctgatctca cttaaatcag cacagtaact 32100 gcagcacagc accacaatat tgttcaaaat cccacagtgc aaggcgctgt atccaaagct 32160 catggcgggg accacagaac ccacgtggcc atcataccac aagcgcaggt agattaagtg 32220 gcgacccctc ataaacacgc tggacataaa cattacctct tttggcatgt tgtaattcac 32280 cacctcccgg taccatataa acctctgatt aaacatggcg ccatccacca ccatcctaaa 32340 ccagctggcc aaaacctgcc cgccggctat acactgcagg gaaccgggac tggaacaatg 32400 acagtggaga gcccaggact cgtaaccatg gatcatcatg ctcgtcatga tatcaatgtt 32460 ggcacaacac aggcacacgt gcatacactt cctcaggatt acaagctcct cccgcgttag 32520 aaccatatcc cagggaacaa cccattcctg aatcagcgta aatcccacac tgcagggaag 32580 acctcgcacg taactcacgt tgtgcattgt caaagtgtta cattcgggca gcagcggatg 32640 atcctccagt atggtagcgc gggtttctgt ctcaaaagga ggtagacgat ccctactgta 32700 cggagtgcgc cgagacaacc gagatcgtgt tggtcgtagt gtcatgccaa atggaacgcc 32760 ggacgtagtc attctcgtac tttgagtggc aaaaccttgc tctcgaacag cacacgtctc 32820 gtcgcctcct gtcccttctc ttcgcctttt cagtgtgata gttgtaatac agccattcac 32880 gaagctcagt cagaagatct tcagcgtctg ttgtcaaaaa caatccatcc aatctgattg 32940 ctttcaaaac atcacaaaca gtcgaataag ccaaacccat ccaggcaatg caattatttt 33000 ggttatccac aatgggaggg ggcggaagac atggaagagg cataattaat tttttaatcc 33060 aatcgatcac gcagcacttc aaaatgaaga tcgcgaaggt gacacctttc acccccactg 33120 tgttgatgaa aaataacagc caagtcaaaa ttgatgcggt tttcaaggtg ctcgactgta 33180 gcatcaagca gagcttccac acgcacgtcc acaaataaca gaatagcaaa agcgggagga 33240 ggaagtaaat cctcaatcat catagtacag tccatcacca tccctaaata attttcatcc 33300 ttccagcctt ggactatatt tttaaactgc tcttgtaaat ccaaaccaca catgtggaaa 33360 agttcccaaa gagctccctc aactaccatt cttaagcaca ccttcatagt gacaaaatat 33420 cttgttcctc tgtcacctgc agcaaattac aaagtccaat attaggatct atgcccagag 33480 atctaagctc atccctcaat tccaactgta aaaaggcttc cagatctgcc ctaacttgtt 33540 cagccagtgg gctccctgga ataagcgtgg gagaagccaa actgcaaaac agacgcatgc 33600 cgccataatt accaccagaa aacactacgt tacagtatgc atgctgattc attccagtaa 33660 tttcatccag tgtattggat acaaaaaaag gcaagcactc tctcactaat tgtattatgg 33720 agacattatc acacaggtaa caatttaaag gttgtggaac aataatgcag taagtaacca 33780 cggtgcgctc caacatggtt agtaattttt agttctgaaa aacaaaacat acaaaaaatt 33840 atatcatact catttggcga actggtggaa aaatgaccct atctagcaca aggcaagcca 33900 ctggatcacc aatgcgcccc tcataaaacc tgtcatcatg attaaaaagc aacaccgaaa 33960 gctcttccct atgtcctgca tgaatgattc tagctgagga atataagcca gcgcaattag 34020 tatctgttaa agaaaaaaaa cggccaacat agcctctagg aattagcaca cttaatctta 34080 aagacattac tgccatcccc cttggattta aggtaaaatt tacaggagca tagaaaatat 34140 actgatttcc ctcctgcaca ggcagcatag caccaggtcc ctctaaaaac acacacaaag 34200 cttctgcagc catagcttac cgcgcaaacc aggcacagca gtgagctaaa aggacaaagc 34260 tctaactcac tagccaacct ggcgcacaat atatagttag tccttacact gacgtaaccg 34320 accaaagtct aaaaaccccg ccaaaaatac acacacgccc aaaaaacgcc ccgtgagtca 34380 aaaaacagtt tcacttcctc gttacaccca aaacgtcgtc acttccggat tcccacggtt 34440 cgtcacttcc ggagctcctt gcttaattaa ccccgcccaa aacgtcatcg tccgcgtcac 34500 gccgccccgc cccgcgaccg ttgaccccgg gccaatcacc gcacatcccg caaaattcaa 34560 actcgtctaa tttgcatatt ggcacactgc ccatataagg tatattattg atgatg 34616

Claims (17)

  1. 서열번호 1의 아미노산 서열 또는 상기 서열번호 1의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 갖는 헥손(hexon) 초가변 영역-포함 폴리펩티드를 포함하는 헥손 폴리펩티드 또는 이의 기능성 유도체를 암호화하는, 단리된 핵산 서열.
  2. 제1항에 있어서, 상기 헥손 폴리펩티드는 상기 서열번호 1의 아미노산 서열을 갖는 헥손 초가변 영역-포함 폴리펩티드를 포함하는, 단리된 핵산 서열.
  3. 제2항에 있어서, 상기 헥손 폴리펩티드 또는 이의 기능성 유도체는 서열번호 2의 아미노산 서열 또는 상기 서열번호 2의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함하는, 단리된 핵산 서열.
  4. 제1항 내지 제3항 중 어느 한 항에 있어서, 섬유 폴리펩티드 또는 이의 기능성 유도체를 암호화하는 핵산 서열을 더 포함하는, 단리된 핵산 서열.
  5. 제4항에 있어서, 상기 섬유 폴리펩티드는: (1) 서열번호 10의 아미노산 서열을 포함하는 섬유 노브(knob) 폴리펩티드 서열, (2) 서열번호 11의 아미노산 서열을 포함하는 섬유 샤프트(shaft) 폴리펩티드 서열, 및 (3) 서열번호 12의 아미노산 서열을 포함하는 섬유 꼬리 폴리펩티드 서열로 이루어진 군으로부터 선택된 적어도 하나를 포함하며, 바람직하게는 상기 섬유 폴리펩티드 또는 이의 기능성 유도체는 서열번호 3의 아미노산 서열 또는 상기 서열번호 3의 아미노산 서열에 대해 전체 길이에 걸쳐 적어도 95% 동일한 아미노산 서열을 포함하는, 단리된 핵산 서열.
  6. (1) 서열번호 10의 아미노산 서열을 포함하는 섬유 노브 폴리펩티드 서열; (2) 서열번호 11의 아미노산 서열을 포함하는 섬유 샤프트 폴리펩티드 서열, 및 (3) 서열번호 12의 아미노산 서열을 포함하는 섬유 꼬리 폴리펩티드 서열로 이루어진 군으로부터 선택된 적어도 하나를 포함하는 섬유 폴리펩티드를 암호화하는, 단리된 핵산 서열.
  7. 제6항에 있어서, 상기 섬유 폴리펩티드는 서열번호 3의 아미노산 서열을 포함하는, 단리된 핵산 서열.
  8. 제1항 내지 제7항 중 어느 한 항의 핵산을 포함하는 벡터.
  9. 제8항에 있어서, 아데노바이러스 벡터이며, 이식유전자를 더 포함하는, 벡터.
  10. 제9항에 있어서, E1 결실 및 E3 결실 중 적어도 하나를 더 포함하는, 아데노바이러스 벡터.
  11. 제9항 또는 제10항에 있어서, 상기 아데노바이러스 벡터는 하나 이상의 인간 아데노바이러스 핵산 서열을 포함하는 키메라 아데노바이러스 벡터이고, 바람직하게는 상기 인간 아데노바이러스 핵산 서열은 인간 아데노바이러스-4, 인간 아데노바이러스-5, 인간 아데노바이러스-26 또는 인간 아데노바이러스-35 중 적어도 하나로부터 유래된, 아데노바이러스 벡터.
  12. 제9항 내지 제11항 중 어느 한 항에 있어서, 서열번호 13 및 서열번호 14로 이루어진 군으로부터 선택된 핵산 서열을 포함하는, 아데노바이러스 벡터.
  13. 제8항 내지 제12항 중 어느 한 항의 벡터를 포함하는 재조합 세포.
  14. 벡터의 생산 방법으로서,
    (a) 상기 벡터의 생산을 위한 조건 하에 제13항의 재조합 세포를 성장시키는 단계; 및
    (b) 상기 재조합 세포로부터 상기 벡터를 단리시키는 단계
    를 포함하는, 벡터의 생산 방법.
  15. 제9항 내지 제12항 중 어느 한 항의 아데노바이러스 벡터를 포함하는, 면역원성 조성물.
  16. 면역 반응의 유도가 필요한 대상체에서의 면역 반응 유도 방법으로서, 제15항의 면역원성 조성물을 상기 대상체에게 투여하는 단계를 포함하는, 면역 반응 유도 방법.
  17. 제9항 내지 제12항 중 어느 한 항에 따른 아데노바이러스 벡터를 약제학적으로 허용 가능한 담체와 조합하는 단계를 포함하는, 백신의 생산 방법.
KR1020207014975A 2017-10-31 2018-10-30 아데노바이러스 및 이의 용도 Ceased KR20200074987A (ko)

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