KR20230077228A - Composition for improving hair loss or promoting hair growth containing a enzyme hydrolysate derived from edible insects and a fermentative product derived from herbal extracts complex - Google Patents

Abstract

The present invention is an edible insect-derived ultra-high pressure and enzyme-treated product; And, it relates to a composition containing; fermented product of composite herbal extracts of kelp, black soybean, medicinal buckwheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam and turmeric. The composition has an excellent antioxidant effect, a high skin inflammation inhibitory effect, and an excellent effect of proliferating various cells related to hair, so it can be easily used as a pharmaceutical and health functional food related composition for preventing hair loss or promoting hair growth.

Description

Composition for improving hair loss or promoting hair growth containing a enzyme hydrolysate derived from edible insects and a fermentative product derived from herbal extracts complex}

The present invention is an edible insect-derived ultra-high pressure and enzyme-treated product; And, it relates to a composition for preventing hair loss or stimulating hair growth containing a fermented product of complex herbal extracts of kelp, black soybean, medicinal wheat, green tea, Rehmannia tuber, perilla leaf, lavender, yam and turmeric.

With the development of an aging society, the number of people suffering from hair loss is increasing. Previously, it was considered a concern of the age group after the age of 50, but recently, an increase in the hair loss population in the young people in their 20s and 30s has been reported. According to the Korean Society for Hair Loss Treatment, the population reaches 10 million, which means that 1 out of 5 Koreans suffer from hair loss, and the number of people who received hospital treatment due to hair loss increased to about 1.12 million from 2016 to 2020. , the number of hair loss patients increased by 10% in 5 years. Hair loss is likely to occur in most men, but according to the data from the National Health Insurance Corporation, 43% (about 100,000) of hair loss patients in 2020 were women.

In the past, it has been known that hair loss has a large genetic influence, and it has been recognized that baldness occurs from generation to generation. Recently, however, it has been revealed that hair loss progresses due to various causes such as stress, Westernized eating habits, nutritional imbalance, and social activity changes along with genetic factors, and among them, nutritional imbalance of the scalp is cited as the biggest cause.

As a treatment method for this hair loss phenomenon, various methods such as topical application, oral medication, health supplement, gene transplant surgery, hair transplant surgery, and alternative medicine have been implemented, but there is no treatment method or substance that shows satisfactory results so far. It is known.

Although hair loss is not a life-threatening disease, due to the recent well-being craze and increased interest in appearance, the market for preventing hair loss or promoting hair growth is showing rapid growth. For example, the market for prescription drugs related to hair loss prevention is reported to be 39 billion won in Korea in 2009, and the total market related to hair loss prevention in 2020 is about 4 trillion won.

Currently, as a drug that exhibits a hair growth effect, those approved by the US FDA are minoxidil (6-Amino-1,2-dihydro-1-hydroxy-2-imino-4-phenoxypyrimidine) for transdermal application and finasteride for oral administration. , Pansidil, etc. In the case of minoxidil, it was developed as a vasodilator for the treatment of high blood pressure in the early 1970s, but it is used as a hair growth promoter as hirsutism has been reported as a side effect, and it shows the effect of thickening the hair by thickening the pores and increasing the diameter of the hair. In addition, in the case of finasteride, it was developed as a treatment for prostatic hyperplasia and is currently being used as a treatment for hair loss, and it slows down the progress of hair loss and exhibits a hair growth effect. It is also a hair loss auxiliary treatment formulated with brewer's yeast and biotin as a base.

However, in the case of minoxidil, side effects such as weight gain, edema, dermatitis, and increased heart rate have been reported. In the case of finasteride, continuous use is required, and side effects such as sexual dysfunction in men and birth defects in pregnant women have been reported. , Pancidil can cause gastrointestinal discomfort such as stomach pain and vomiting, tachycardia, itching and hives, and side effects such as headache and dizziness can be accompanied, so the development of hair loss treatment without side effects is still required. am.

Accordingly, a variety of researches are being conducted to develop products containing active ingredients beneficial to scalp health by exploring natural ingredients that do not contain chemical components. A lot of research on ultra-high pressure treatment or enzymatic hydrolysis to improve the

For example, Korean Patent No. 10-1779480 describes a composition for improving hair loss and promoting hair growth containing fermented medicinal beans, and Korean Patent No. 10-1770420 contains fermented black ginseng extract as an active ingredient to prevent hair loss, prevent dandruff, and prevent scalp itchiness. Detergent composition to relieve hair loss, Korean Patent Registration No. 10-1762796 discloses a composition for preventing hair loss comprising a fermented product of a compound herbal extract. In addition, Korean Patent No. 10-1653140 discloses a composition for preventing hair loss and promoting hair growth containing Eoseongcho, and Korean Patent No. 10-1704645 discloses a mixture of black beans, brown rice and rice bran mixed with white hazelnut and Angelica quai. Fermented enzyme foods containing hair loss prevention and hair growth promoting effects are disclosed, and Korean Patent Registration No. 10-1414150 discloses anti-wrinkle cosmetic compositions containing a placenta extract hydrolyzed by an ultra-high pressure system. Studies on insect-derived hair loss prevention or hair growth promoting effects are insignificant.

Edible insects, such as twin crickets, brown mealworm larvae, and white-spotted flower larvae, are protein-rich. It has a high content, unsaturated fatty acids and essential fatty acids, so it is evaluated as suitable for future food, and the UN and the International Food and Agriculture Organization have announced that edible insects are applicable as future food resources. In Korea, 10 species of insects, including twin crickets, are currently recognized as food ingredients, and research on food products using these insects is being actively conducted.

Kelp is a large seaweed of the kelp family, and the 2- to 4-year-old thallus is a spore generation, and the stem, leaf, and root are clearly distinguished from the outside. Kelp contains fucoidan, alginic acid, a type of dietary fiber, protein and amino acids, calcium, iodine, and glutamine.

Black soybean is a soybean whose grain shell is black in color. Compared to general soybeans, it has more anti-aging ingredients and is effective in preventing adult diseases and dieting. Black beans contain 40% protein, 35% carbohydrates (25% dietary fiber, 10% oligosaccharides), 20% lipids, 5% vitamins, calcium, lecithin, and isoflavones.

The medicinal buckwheat is a dicotyledonous perennial plant of more than 300 family. The main active ingredients of medicinal wheat are essential oil and flavonoid compounds, and other alkaloids, lignans, organic acids, etc. are known to have antibacterial, anti-inflammatory, antihypersensitivity, and immune function enhancement.

Green tea contains a large amount of polyphenols that act as antioxidants, and catechins in green tea have anticancer effects, blood vessel health, stomach cancer, and lung cancer prevention effects, lower blood pressure, and reduce cholesterol in the digestive tract. It is known to inhibit absorption and inhibit the deposition of lipids in the body.

Rehmannia glutinosa is a dried or boiled root of ginseng and its related plants, which is used for medicinal purposes. It contains catalpol and mannit, promotes blood coagulation, increases contractility of heart muscle against a weak heart, and has diuretic and antipyretic effects. It suppresses experimentally induced hyperglycemia and also lowers blood sugar in normal rabbits.

Perilla leaf is the leaf and end branch of the tea stage or wrinkled leaflet of Lamiaceae. It contains about 0.5% essential oil. It is effective in antioxidant, anti-inflammatory, immunomodulatory, soothing, and antibacterial, and exhibits strong anti-inflammatory (resistance to inflammation) and immune-enhancing effects on the skin.

Lavender is a perennial plant belonging to the Lamiaceae family. It is cultivated to obtain fragrance oil from flowers and plants. It is known to be effective in antidepressants, stimulating energy, strengthening nerves, promoting recovery, sedation, and promoting cell growth.

Yam is a herbal medicine made from the root of a vine perennial plant of Maaceae. It contains mucus protein and is rich in various enzymes, especially amylase. It is said to be a sanyak and is used as a tonic, and is known to have the effect of protecting the kidneys, strengthening the five organs, and strengthening muscles and bones from the symptoms of weakening and exhausting the body.

Turmeric is an annual plant of the ginger family, and its stems and roots are used for food and medicine. Curcumin helps secretion of bile and digestion. It is also known to be effective for blood circulation and nosebleeds, and to help prevent dementia.

Accordingly, the inventors of the present invention confirmed that a composition containing an edible insect-derived ultra-high pressure and enzyme-treated product and a fermented product of a composite herbal extract has an effect of improving hair loss or promoting hair growth while conducting research on food production using edible insects and natural products. It was possible to complete the present invention.

Republic of Korea Patent Registration No. 10-1779480 (Title of invention: Composition for improving hair loss and promoting hair growth containing fermented medicinal beans, Applicant: Seoul National University Industry-University Cooperation Foundation, registration date: September 12, 2017) Republic of Korea Patent Registration No. 10-1779480 (Title of Invention: Detergent composition containing fermented black ginseng extract as an active ingredient to promote hair growth, prevent hair loss, prevent dandruff, and relieve scalp itchiness, Applicant: Ginseng Byfarm Co., Ltd., Registration date: 2017 08/16/2018) Republic of Korea Patent Registration No. 10-1779480 (Title of Invention: Composition for preventing hair loss containing fermented herbal extracts, Applicant: Korea Institute of Oriental Medicine, registration date: July 24, 2017) Republic of Korea Patent Registration No. 10-1779480 (Title of Invention: Composition for preventing hair loss and promoting hair growth containing fermented medicinal extracts, Applicant: Saerombio Co., Ltd., registration date: April 19, 2016) Republic of Korea Patent Registration No. 10-1779480 (Title of Invention: Fermented enzyme food with hair loss prevention and hair growth promoting effects, Applicant: Hi-Mo Limited Liability Company, Registration Date: February 20, 2017) Republic of Korea Patent Registration No. 10-1414150 (Title of Invention: Anti-wrinkle cosmetic composition containing placenta extract hydrolyzed by ultra-high pressure system, Applicant: Kyeonkuk University Industry-Academic Cooperation Foundation, Registration Date: June 25, 2014)

An object of the present invention is an edible insect-derived ultra-high pressure and enzyme-treated product; And, to provide a composition for preventing hair loss or stimulating hair growth containing; and a fermented product of complex herbal extracts of kelp, black soybean, medicinal wheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam and turmeric.

The present invention is an edible insect-derived ultra-high pressure and enzyme-treated product; And, it relates to a composition for preventing hair loss or stimulating hair growth containing a fermented product of complex herbal extracts of kelp, black soybean, medicinal wheat, green tea, Rehmannia tuber, perilla leaf, lavender, yam and turmeric.

The edible insect may be at least one selected from the group consisting of paired crickets, brown mealworm larvae, and white-spotted flower larvae.

The edible insect-derived ultra-high pressure and enzyme-treated material is characterized in that the edible insect, which is a raw material sample, is treated with enzyme after ultra-high pressure treatment at a pressure of 100 to 1,000 MPa and a temperature of 40 to 90 ° C. for 5 to 60 minutes. The enzyme may be an enzyme selected from protamax, Flavozyme, Alcalase, Protease, BAN (Bacterial Amylase Novo), or α-Amylase. there is.

The present invention provides a health functional food for preventing hair loss or promoting hair growth containing the composition.

The present invention also provides a pharmaceutical composition for treating hair loss containing the composition.

As another aspect, the present invention may provide a cosmetic composition for preventing hair loss or promoting hair growth containing the composition.

Hereinafter, the present invention will be described in detail.

The composition is based on 100 parts by weight of 'edible insect-derived ultra-high pressure and enzyme treatment', 30 to 300 parts by weight of 'fermented product of compound herbal extracts of kelp, black soybean, medicinal wheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam and turmeric' can be included Preferably, ultra-high pressure and enzyme-treated products derived from edible insects; And, the fermented product of the composite herbal extract; may be included in the same weight.

The edible insect-derived ultra-high pressure and enzyme-treated product is preferably,

(First step) fasting, washing, and dehydrating edible insects; (Second step) pre-treatment by heating the washed and dehydrated edible insects by irradiating mid-infrared rays; (Third step) drying the pretreated edible insects with hot air; (Fourth step) obtaining degreased powder of edible insects by pressing and milking edible insects dried with hot air; (Step 5) subjecting degreasing powder of edible insects to ultra-high pressure; (Step 6) Steaming and enzymatically processing the ultra-high pressure treated powder; and (Step 7) obtaining an enzyme-treated product by centrifuging the enzyme-treated sample and freeze-drying the supernatant.

In the method for producing the edible insect-derived ultra-high pressure and enzyme-treated product,

In the second step, mid-infrared rays are irradiated for 5 to 10 minutes, and it is preferable to maintain an internal temperature of 120 to 150 ° C through mid-infrared rays irradiation. At this time, if the mid-infrared irradiation time is less than 5 minutes, the taste and flavor of the finally prepared edible insect larvae or imago defatted powder may be bad, and if the mid-infrared irradiation time exceeds 10 minutes, benzopyrene may be generated, which is not preferable. . In this case, a mid-infrared irradiator manufactured for drying food may be used.

In the method for producing an ultra-high pressure and enzyme-treated product derived from edible insects, even when the second and third steps are reversed, benzopyrene can be detected in the finally prepared edible insect larva or adult powder.

In the third step, hot air drying can be performed at 60 to 80 ° C for 6 to 10 hours. When hot air drying is performed at less than 60 ° C or less than 6 hours, hot air drying does not work well, so moisture in the larvae can be included On the other hand, when hot air drying exceeds 80 ° C. or is performed for more than 10 hours, benzopyrene may be generated, which is not preferable.

The milking process of the fourth step may be performed at 50 to 90 ° C for 20 to 50 minutes at a pressure of 500 to 700 kgf / cm ² . At this time, if the pressure during milking is less than 500kgf/cm ² , the oil in the larva or adult powder of edible insects may not be well removed, and if the pressure exceeds 700kgf/cm ² , more pressure than necessary is applied. Not desirable.

Grinding after milking of edible insect larvae or adults can be pulverized so that the size of the powder particles is 50 to 70 mesh.

The ultra-high pressure treatment process of the fifth step may be performed at a pressure of 100 to 1,000 MPa. More specifically, it can be treated for 5 to 60 minutes at a pressure of 100 to 1,000 MPa and a temperature of 40 to 90 ° C. At this time, if the pressure is less than 100 MPa, the effect of the ultra-high pressure treatment (increase in active ingredient content, etc.) is insignificant, and if it exceeds 1,000 MPa, the pressure is too high and the work may be difficult. In addition, if the temperature is less than 40 ° C., the treatment efficiency is reduced, and if it exceeds 90 ° C., it may have a negative effect due to component reduction due to high heat transfer. In addition, if the treatment time is less than 5 minutes, the effect of the ultra-high pressure treatment is insignificant, and if it exceeds 60 minutes, the synergistic effect is not great.

The pressure steaming in the sixth step is performed by adding 200 to 400 parts by weight of purified water based on 100 parts by weight of degreased edible insect larvae or adults, followed by heating at a pressure of 1.1 to 1.5 kgf/cm ² and 110 to 150 ℃ for 0.5 to 8 hours. It is done by softening. When adding purified water, if the purified water is less than 200 parts by weight based on 100 parts by weight of larvae or adults of edible insects, the quality of the proteolytic composition may be reduced due to overactivation of the enzyme in the subsequent enzyme treatment process, and 400 parts by weight of purified water If it is exceeded, proteolysis may not be performed well due to weak enzyme activity during subsequent enzyme treatment.

In the heating condition, if the heating temperature is less than 110 ° C. during the softening process, the softening action may not occur well, and if the heating temperature exceeds 150 ° C., the properties of the protein may change and the quality of the proteolyzed composition may deteriorate. . Likewise, if the heating time is less than 0.5 hours, softening may not be performed well, and if the heating time exceeds 8 hours, the properties of the protein may change.

In the step of preparing the enzyme-treated material, after adjusting the pH of the pressure-cooked edible insects (liquid state) to 7.0, 0.5 to 5 parts by weight of enzyme based on 100 parts by weight of edible insects is added, and the enzyme at 30 to 37 ° C. for 0.5 to 48 hours. can be done in response. At this time, the activity of the enzyme can be stopped by heating after the enzyme reaction, and it is preferable to heat-treat at 90 to 110 ° C. for 5 to 20 minutes. Enzymatic treatment may be performed by only one type of enzyme or by mixing two or more types of enzymes to be treated simultaneously or sequentially.

The enzyme-treated product of the seventh step can be obtained by drying after hydrolysis and concentrating the supernatant under reduced pressure.

The fermented product of the composite herbal extract may be obtained by fermenting an extract obtained by mixing kelp, black soybean, medicinal buckwheat, green tea, Rehmannia tuber, perilla leaf, lavender, yam and turmeric with a yeast strain and a lactic acid bacteria strain. Preferably, the fermentation Complex crude drug extract, which is the raw material of water, contains 10-20% by weight of kelp, 10-20% by weight of black soybean, 10-20% by weight of medicinal buckwheat, 10-20% by weight of green tea, 5-15% by weight of Rehmannia glutinosa, and 5-15% by weight of perilla leaf. , It may be an extract containing 3 to 7% by weight of lavender, 3 to 7% by weight of yam, and 5 to 15% by weight of turmeric. The composite herbal extract can be prepared using a general extraction method, separation and purification method. The extraction method is not limited thereto, but specifically, methods such as hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction may be used.

Preparation of the fermented product of the composite herbal extract is preferably (step A) mixing each herbal medicine to obtain a composite; (Step B) extracting the complex to obtain a composite herbal medicine extract; (Step C) Fermenting the composite crude drug extract with yeast and lactic acid bacteria; and (D step) filtering and concentrating the fermentation product.

To explain in more detail, in the present invention, the composite crude drug extract used in the production of the fermented product is prepared by adding 2 to 20 times the weight of an extraction solvent (preferably, water) to the extraction raw material for 8 to 48 hours at 80 to 98 ° C. It may be an extract obtained by extraction during extraction, and more preferably, the filtrate obtained by removing the solids from the extract and filtering may be concentrated under reduced pressure at 40 to 60 ° C. to produce a concentrate of 15 to 20 Brix.

The fermented product of the composite crude drug extract may be obtained by primary fermentation by adding yeast to the composite herbal drug extract (extract concentrate) obtained by concentration under reduced pressure, and then secondary fermentation by additionally adding lactic acid bacteria.

Each treatment condition of the yeast and lactic acid bacteria is preferably 2 to 90 hours at 20 to 80 ° C. When the temperature exceeds 80 ° C., the enzyme activity in these strains is inactivated. Yeast and lactic acid bacteria may be added in an amount of 0.1 to 5 parts by weight based on 100 parts by weight of the composite crude drug extract. After the fermentation of lactic acid bacteria is completed, fermentation can be stopped by heating at 90 to 100 ° C. for 20 to 40 minutes, and the fermented product can be powdered by concentrating and drying under reduced pressure.

The yeast may be selected from at least one selected from among Saccharomyces serevisiae and Saccharomyces boulardii , preferably Saccharomyces serevisiae . It can be.

The lactic acid bacteria may be at least one selected from the group consisting of Lactobacillus plantarum , Lactobacillus thermophilus and Leuconostoc mesenteroises , preferably Lactobacillus Plantarum ( Lactobacillus plantarum ) may be selected.

In the preparation of the fermented product of the composite crude drug extract of the present invention, in step A, each raw material of kelp, black soybean, medicinal buckwheat, green tea, Rehmannia tuber, perilla leaf, lavender, yam, and turmeric is pulverized into particles having a size of 30 to 50 mesh, and then kelp 10-20% by weight, black soybean 10-20% by weight, medicinal wheat 10-20% by weight, green tea 10-20% by weight, Rehmannia Root 5-15% by weight, perilla leaf 5-15% by weight, lavender 3-7% by weight, yam It can be prepared as a composite herbal medicine by mixing 3 to 7% by weight and 5 to 15% by weight of turmeric. At this time, if each raw herbal medicine particle exceeds 50 mesh, the extraction efficiency may decrease, and if it is less than 30 mesh, there is a disadvantage in that there is difficulty in work.

In step B, 200 to 2000 parts by weight of water is added based on 100 parts by weight of the composite herbal medicine, extracted at 80 to 98 ° C for 8 to 48 hours, filtered to obtain a concentrate with 15 to 20 brix, and then fermented. .

At this time, if the purified water is less than 200 parts by weight based on 100 parts by weight of the pulverized compound herbal medicine, the extraction efficiency is reduced, and if it is more than 2000 parts by weight, there is an uneconomical disadvantage in the concentration process. In addition, when the extraction temperature is less than 80 ℃, there is a disadvantage that the extraction time is long, while if it is more than 98 ℃, the component change may occur.

In step C, the preparation of the fermented product of the compound herbal extract is based on 100 parts by weight of the compound herbal extract (concentrate) of 15 to 20 brix kelp, black soybean, medicinal wheat, green tea, Rehmannia tuber, perilla leaf, lavender, yam and turmeric Saccharomyces By adding 0.1 to 5 parts by weight of a strain of the genus Seth, primary fermentation can be performed under the conditions of pH 6-7, temperature 20-50 ° C, incubation time 2-90 hr. In addition, 0.1 to 5 parts by weight of Lactobacillus genus strains based on 100 parts by weight of composite crude drug extract (concentrate) is added to the primary fermentation product, and secondary fermentation is performed under the conditions of a temperature of 20 to 50 ° C and a culture time of 2 to 90 hr. complete Thereafter, the fermentation can be stopped by heating the fermented product in a water bath at 80 to 100 ° C for 20 to 40 minutes. After that, it is concentrated and dried at 40 to 50 ° C. to prepare a fermented product of the composite crude drug extract.

If the amount of each strain is added below the lower limit, the activity of the strain may hardly appear, and adding in excess of the upper limit is undesirable in terms of cost for using the strain, and the action of the strain becomes excessive, resulting in final decomposition. The physical properties of the product may deteriorate.

The fermented product of the composite crude drug extract can be heated to complete fermentation, filtered with paper, non-woven fabric, cloth, etc. to filter out residues, and then dried. This may be performed through a hot air drying method, a spray drying method, a freeze drying method, or a room temperature (20 to 30° C.) drying method.

In the preparation of the extract before fermentation in the present invention, the extraction may be repeated 1 to 4 times. As a solvent for preparing the extract, water, C1-C4 alcohol, or a mixture thereof may be used as a solvent for extraction. The C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol. As the extraction solvent, water is most preferably used.

As a device for extracting the extract, a conventional extraction device, an ultrasonic crusher, or a fractionator may be used. The extract thus prepared may be subjected to hot air drying, vacuum drying or freeze drying to remove the solvent. In addition, the extract can be used after being purified using column chromatography.

The present invention is an edible insect-derived ultra-high pressure and enzyme-treated product; And, fermented product of kelp, black soybean, medicinal buckwheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam and turmeric composite herbal extract; provides a composition of health functional food containing hair loss improvement or hair growth promoting effect. The health functional food includes tablets, capsules, pills, or liquid forms, and foods to which the extract of the present invention can be added include, for example, various drinks, meat, sausages, bread, candies, snacks, and noodles. , ice cream, dairy products, soups, ionic beverages, soft drinks, alcoholic beverages, chewing gum, tea, and vitamin complexes.

In addition, the present invention is edible insect-derived ultra-high pressure and enzyme treatment; And, it provides a pharmaceutical composition for treating hair loss containing; fermented product of kelp, black soybean, medicinal buckwheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam and turmeric composite herbal extract. The pharmaceutical composition may be formulated and used in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and sterile injection solutions according to conventional methods. . Carriers, excipients, and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, and cellulose. , methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations include at least one excipient, for example, starch, calcium carbonate, sucrose or lactose in the pharmaceutical composition of the present invention. , gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and calt are also used. Liquid preparations for oral use include suspensions, soy solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. can Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for suppositories, Witepsol, Macrogol, Tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, and weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, the degree of administration, and the prescriber's judgment. Dosage determination based on these factors is within the level of those skilled in the art, and generally dosages range from 0.01 mg/kg/day to approximately 2,000 mg/kg/day. A more preferred dosage is 1 mg/kg/day to 500 mg/kg/day. Administration may be administered once a day, or may be administered in several divided doses. The dosage is not intended to limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, and humans through various routes. All modes of administration are contemplated, eg oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine intrathecal or intracerebrovascular injection. Since the composition of the present invention has little toxicity and side effects, it is a drug that can be safely used even when taken for a long period of time for preventive purposes.

In the present invention, edible insect-derived ultra-high pressure and enzyme treatment; And, a fermented product of kelp, black soybean, medicinal buckwheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam, and turmeric complex herbal extract; it is possible to provide a cosmetic composition having hair loss prevention or hair growth promoting efficacy. The cosmetic composition of the present invention can be prepared in any formulation that is conventionally prepared, for example, solutions, emulsions, suspensions, pastes, creams, lotions, gels, powders, sprays, surfactant-containing cleansers, oils, and soaps. , liquid cleanser, bath salt, foundation, makeup base, essence, lotion, foam, pack, softening water, sunscreen cream, sun oil, shampoo, conditioner, hair treatment, hair gel, hair mousse, hair spray, hair lotion, hair pack , hair essence, etc., but is not limited thereto.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, or tracanth and the like may be used.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

The carrier may be a non-naturally occurring carrier.

Hair loss referred to in the present invention includes androgenetic alopecia (baldness), alopecia areata, tinea capitis due to fungal infection, telogen effluvium, hair growth, hair growth disorders, etc., and scarring alopecia includes hair loss caused by lupus. , solitary folliculitis, lichen planus pilaris, and alopecia caused by burns and trauma.

The present invention is an edible insect-derived ultra-high pressure and enzyme-treated product; And, it relates to a composition containing; fermented product of composite herbal extracts of kelp, black soybean, medicinal buckwheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam and turmeric. The composition has an excellent antioxidant effect, a high skin inflammation inhibitory effect, and an excellent effect of proliferating various cells related to hair, so it can be easily used as a pharmaceutical and health functional food related composition for preventing hair loss or promoting hair growth.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the invention to those skilled in the art, so that the disclosure herein will be thorough and complete.

<Preparation Example 1. Preparation of enzyme-treated product of brown mealworm larvae>

Preparation Example 1-1. Preparation of Protamx and BAN Enzyme Treatments of Brown Goose Larvae

Brown mealworm larvae of size 9 were purchased from specialized breeding farms, and the brown mealworm larvae were fasted for 3 days, respectively, and the excrement was completely discharged, and washed with running water three times in a raw state. Thereafter, manufacturing of the high temperature/pressure and enzyme-treated material is completed through a drying step, a degreasing step, a grinding step, a high temperature/pressure step, an enzyme hydrolysis step, and a drying step. Among them, the drying step, the degreasing step, and the grinding step were performed through the following process.

① Drying step: Using an industrial mid-infrared ray dryer (MS3-6, Lichtzen, Korea) equipped with a lamp that emits peak energy in the MIR 3㎛ band, treat at 140 ° C for 6 minutes, and then dry the moisture of brown mealworm larvae It was dried using a hot air dryer for 12 hours at a drying temperature of 70 ° C. so that the content was 5% or less.

② Degreasing step: Insects in a degreasing state were obtained by milking for 20 minutes while applying a pressure of 600 kgf/cm ² using an industrial milking machine (Myeongjin Machinery). The adhered oil (sample amount) was 2 kg, and the adhered oil (inside the milking machine) temperature was 60°C.

③ Grinding step: It was pulverized with an industrial pink crusher (fin mill, Sungchang machine) to a particle size of 50 to 70 mesh for brown mealworm larvae, and used as a raw material for enzyme treatment.

④ Ultra-high pressure treatment: Add about twice as much distilled water to 200 g of pulverized brown beggar larvae powder, seal well to prevent air from entering, and then use an ultra-high pressure processing device (055-60, Uhde, Germany) to process 50 It was treated for 5 minutes at a pressure of 500 MPa at °C.

⑤ Treatment of brown mealworm larvae Pressurized steaming: Afterwards, the brown mealworm larvae pulverized material subjected to ultra-high pressure treatment is put into a steamer, and then purified water three times the weight of the pulverized material is added to the conditions of a temperature of 121 ° C and a pressure of 1.3 kgf / cm ² pressure steamed for 3 hours.

⑥ Production of enzymatically treated brown mealworm larvae: 1 part by weight of Protamax (Subtilisin Carlsberg, Novozymees) per 100 g of ground brown mealworm larvae treated with ultra-high pressure and pressure steaming was first hydrolyzed for 5 hours at pH 7.0 and 50 ° C. After inactivation at 98°C for 30 minutes, 1 g of BAN (alpha Amylase Novo) was added to the primary hydrolyzate, followed by secondary hydrolysis at pH 7.0 and 75°C for 10 hours. After that, it was deactivated by heat treatment at 98 ° C. for 30 minutes, and then filtered and dried to obtain a powdered brown mealworm larvae hydrolyzate.

Preparation Example 1-2. Preparation of Protamx enzyme treatment of brown mealworm larvae

In Preparation Example 1, the second enzyme treatment was not performed, but the first enzyme treatment was performed for 15 hours.

Preparation Example 1-3. Manufacture of BAN enzyme treatment of brown mealworm larvae

In Preparation Example 2, BAN (Alpha Amylase Novo) was used instead of Protamax to obtain a BAN hydrolyzate of brown mealworm larvae.

<Comparative Preparation Example 1. Preparation of Comparative Composition of Brown Goose Larvae>

Comparative Preparation Example 1-1. Production of Protamx enzyme-treated material of brown mealworm larvae omitted from ultra-high pressure treatment

Among the steps of Preparation Example 1, brown mealworm larvae were prepared as steamed water by performing steps ①②③ and ⑤ (ultra-high pressure treatment step omitted), and then enzymatic treatment step ⑥ was performed to obtain brown mealworm larvae hydrolyzate.

Comparative Preparation Example 1-2. Manufacture of ultra-high pressure treatment of brown mealworm larvae

Among the steps of Preparation Example 1, the ultra-high pressure treatment step and the pressure steaming step of the brown mealworm larvae were performed (①②③④ and ⑤), and the enzyme treatment was omitted, but immediately after adding purified water of 5 times the weight of the brown mealworm larva, 8 After extraction for an hour, it was filtered and dried to prepare an extracted powder of brown gourd larvae.

Comparative Preparation Example 1-3. Manufacture of pressurized processing of brown mealworm larvae

Among the steps of Preparation Example 1, the brown mealworm larvae were prepared in steps ①②③ and ⑤ to extract the steamed product at 95 ° C. for 8 hours, then filter and dry to prepare an extracted powder of brown mealworm larvae (ultra-high pressure treatment step and enzymatic treatment step omitted).

<Experimental Example 1. Total polyphenol content of composition derived from brown gourd larvae>

When raw materials such as ginseng are treated with ultra-high pressure, it is known that not only the total flavonoid and polyphenol content increases, but also the antioxidant activity. Total flavonoid contents of 1-1 to Comparative Preparation Examples 1-3 were measured.

For the measurement method, 10 ml of 70% ethanol was mixed with 1 g of each sample, homogenized three times for 30 seconds using a homogenizer (IKA T25 Digital Ultra-Tumax, Staufen, Germany), and then extracted for 24 hours. The extract was centrifuged at 3000xg at 4°C for 15 minutes, and the supernatant was used as a sample. The content of total polyphenols was measured according to the Folin-Denise method (Folin and Denis, 1912), and then the content was determined by a standard curve using gallic acid (Sigma-Aldrich Co., St. Louis, MO, USA) as a standard material. was measured. The measurement results are shown in Table 1.

As a result of the analysis, in Preparation Example 1-1, in particular, the total polyphenol content was 8.62 mg/g GAE, which was increased by more than 3 mg/g GAE compared to Comparative Preparation Example 1-1, confirming that the antioxidant efficacy was enhanced in the ultra-high pressure treated water. . (*GAE : gallic acid equivalent).

sample Total polyphenol content (mg/g GAE) ^* Preparation Example 1-1 8.62±0.29 Comparative Preparation Example 1-1 5.51±0.29 Comparative Preparation Example 1-2 7.09±0.38 Comparative Preparation Example 1-3 5.49±0.29

Experimental Example 2. Amino acid content of enzymatically treated product of brown gourd larvae

Amino acid contents of Preparation Examples 1-1 to 1-3 and Comparative Preparation Examples 1-1 to 1-3 were investigated.

As for the analysis method, 20 mL of dilution buffer (pH 2.2) for amino acid analysis was added to 0.2 g of each sample powder, sonicated twice for 20 minutes, and then centrifuged. The supernatant was taken, filtered through a membrane filter (0.45 ㎛), diluted to an appropriate concentration, and used as a sample for free amino acid analysis. 0.1 g of the protein degradation sample was added with 20 mL of dilution buffer (pH 2.2) for amino acid analysis, and then extracted in the same manner as above to be used as a sample for analysis. As an amino acid analyzer, Sykam (Germany)'s S7130 amino acid reagent organizer, S5200 sample injector and S2100 solvent delivery system were used, and the column was analyzed using a cation separation column LCA K06/NA (4.6 × 250 mm). The flow rates of the mobile phase were analyzed at 0.45 mL/min and ninhydrin at 0.4 mL/min, and the analysis results were expressed as mg per 1 g of sample (mg/g).

division Preparation Example 1-1 Preparation Example 1-2 Preparation Example 1-3 comparison
Preparation Example 1-1 comparison
Preparation Example 1-2 comparison
Preparation Example 1-3 Leucine, Isoleucine and valine (mg/g) (BCCA: involved in muscle building) 9.76 8.02 8.34 7.65 5.02 0.57 Methionine, Leucine and Lysine (mg/g) (MLL: involved in collagen synthesis) 10.35 9.43 9.52 8.21 4.89 0.52 Essential free amino acids (mg/g) 20.47 18.20 18.48 12.75 7.43 0.73 Nonessential free amino acids (mg/g) 39.72 37.21 38.12 24.44 22.28 4.21 Total free amino acids (mg/g) 60.19 55.412 56.60 37.19 29.71 4.94

As a result of the analysis, as shown in Table 2, it is expected that the ultra-high pressure/enzyme treatment of brown gourd larvae of Preparation Example 1-1 is rich in amino acids related to hair loss prevention and hair growth, and thus has a positive effect on hair loss prevention and hair growth. In addition, the values of Preparation Example 1-2 and Preparation Example 1-3 are also confirmed to be good.

In addition, as a result of repeated experiments, in the conditions of Preparation Example 1-1 to Preparation Example 1-3, Leucine, Isoleucine and valine were approximately 8.0 to 11.0 mg/g, Methionine, Leucine and Lysine were 9.0 to 12.0 mg/g in the powder. , 17.0-23.0 mg/g of essential free amino acids, 35.0-42.0 mg/g of non-essential free amino acids, and 53.0-62.0 mg/g of total free amino acids were found to be included. In addition, with the powder of Preparation Example 1-1 alone, Leucine, Isoleucine, and Valine were 9.0 to 11.0 mg/g, Methionine, Leucine, and Lysine were 10.0 to 12.0 mg/g, essential free amino acids were 20.0 to 23.0 mg/g, and non-essential free amino acids were 39.0 mg/g. ~ 42.0 mg/g, total free amino acids 59.0 ~ 62.0 mg/g were found to be included.

<Preparation Example 2 and Comparative Preparation Example 2. Preparation of composite herbal extract or fermented product thereof>

Kelp, black soybean, medicinal buckwheat, green tea, Rehmannia tuber, perilla leaf, lavender, yam, and turmeric (Gyeongdong Market, Seoul, Jegi-dong) were purchased and used as extraction raw materials. Each herbal medicine was mixed as shown in the following Table 1, purified water 10 times the weight of the total weight of the extraction raw material was added, extracted at 90 ° C for 12 hours, and the obtained liquid was filtered and concentrated to 15-20 brix to obtain a composite herbal extract.

Thereafter, 2 parts by weight of Saccharomyces serevisiae was added based on 100 parts by weight of the concentrated composite crude drug extract, and primary fermentation was performed under conditions of pH 6-7, temperature 50 ° C, and incubation time of 24 hours. After heating in a 100 ℃ water bath for 10 minutes to stop the fermentation to obtain the first fermented product. Thereafter, 2 parts by weight of Lactobacillus plantarum based on 100 parts by weight of the concentrated composite crude drug extract was added to the primary fermentation product, and secondary fermentation was completed under conditions of a temperature of 37 ° C and a culture time of 24 hours. Similarly, the fermented product was heated in a 100° C. water bath for 10 minutes to stop fermentation, and concentrated under reduced pressure at 50° C. to prepare a fermented product.

In addition, as shown in Table 3 below, each step is performed in the same way, but in the comparative preparation example, each composition is prepared by presenting a different mixing ratio of crude drugs, or fermentation conditions are only yeast fermentation or lactic acid bacteria fermentation It was compared with a method in which fermentation was not performed at all, or fermentation was performed by changing the fermentation step.

Kelp
(g) black bean
(g) buckwheat
(g) green tea
(g) Rehmannia
(g) perilla leaf
(g) lavender
(g) yam
(g) curcuma
(g) total
(g) fermentation order No. 1
fermentation 2nd
fermentation Preparation Example 2-1 15 15 15 15 10 10 5 5 10 100 leaven Lactobacillus Preparation Example 2-2 20 10 10 20 5 15 7 7 6 100 leaven Lactobacillus Preparation Example 2-3 10 20 20 11 15 5 7 7 5 100 leaven Lactobacillus Preparation Example 2-4 12 14 12 14 12 15 7 3 11 100 leaven Lactobacillus Preparation Example 2-5 13 17 18 10 7 10 3 7 15 100 leaven Lactobacillus comparison
Preparation Example 2-1 11 12 11 11 11 11 11 11 11 100 Lactobacillus fermentation only comparison
Preparation Example 2-2 11 12 11 11 11 11 11 11 11 100 leaven
fermentation only comparison
Preparation Example 2-3 15 15 15 15 10 10 5 5 10 100 Lactobacillus leaven comparison
Preparation Example 2-4 15 15 15 15 10 10 5 5 10 100 no fermentation comparison
Preparation Example 2-5 0 20 0 20 0 30 0 30 0 100 leaven Lactobacillus comparison
Production Example 2-6 30 0 30 0 20 0 20 0 0 100 leaven Lactobacillus comparison
Production Example 2-7 50 20 5 5 5 5 5 5 0 100 leaven Lactobacillus comparison
Production Example 2-8 5 40 25 5 5 5 5 5 5 100 leaven Lactobacillus

< Example 1 to 7; comparative example 1 to 17. brown mealworm larval Enzyme treatment and herbal medicine fermented Preparation of Mixed Composition>

A mixed formulation was prepared by mixing the brown mealworm larvae-derived composition and the herbal medicine-derived composition in the same weight as shown in Table 4 below, and a single composition or mixed formulation was prepared by varying the mixing ratio as shown in Table 5.

[Processed product of brown mealworm larvae + crude drug composition] Equal weight mixed formulation Example 1 'Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-1' fermented herbal medicine Example 2 'Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-2' fermented herbal medicine Example 3 'Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-3' fermented herbal medicine Example 4 'Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-4' fermented herbal medicine Example 5 'Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-5' fermented herbal medicine Example 6 'Preparation Example 1-2' enzyme-treated product + 'Preparation Example 2-1' fermented herbal medicine Example 7 'Preparation Example 1-3' enzyme-treated product + 'Preparation Example 2-1' fermented herbal medicine Comparative Example 1 'Comparative Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-1' fermented herbal medicine Comparative Example 2 'Comparative Preparation Example 1-2' ultra-high pressure treated product + 'Preparation Example 2-1' fermented herbal medicine Comparative Example 3 'Comparative Preparation Example 1-3' pressurized product + 'Preparation Example 2-1' fermented herbal medicine Comparative Example 4 'Preparation Example 1-1' Larva Enzyme Treatment + 'Comparative Preparation Example 2-1' Fermented Herbal Medicine Comparative Example 5 'Preparation Example 1-1' larval enzyme treatment + 'Comparative Preparation Example 2-2' fermented herbal medicine Comparative Example 6 'Preparation Example 1-1' larval enzyme treatment + 'Comparative Preparation Example 2-3' fermented herbal medicine Comparative Example 7 'Preparation Example 1-1' larvae enzyme-treated product + 'Comparative Preparation Example 2-4' crude drug extract Comparative Example 8 'Preparation Example 1-1' larval enzyme treatment + 'Comparative Preparation Example 2-5' fermented herbal medicine Comparative Example 9 'Preparation Example 1-1' larval enzyme treatment + 'Comparative Preparation Example 2-6' fermented herbal medicine Comparative Example 10 'Preparation Example 1-1' Larva Enzyme Treatment + 'Comparative Preparation Example 2-7' Fermented Herbal Medicine Comparative Example 11 'Preparation Example 1-1' larval enzyme treatment + 'Comparative Preparation Example 2-8' fermented herbal medicine Comparative Example 12 'Comparative Preparation Example 1-1' enzyme-treated product + 'Comparative Preparation Example 2-1' fermented herbal medicine Comparative Example 13 'Comparative Preparation Example 1-1' Larva Enzyme Treatment + 'Comparative Preparation Example 2-4' Herbal Medicine Extract

[Processed product of brown mealworm larvae], [herbal medicine composition] or a mixture thereof Comparative Example 14 'Preparation Example 1-1' enzyme-treated product Comparative Example 15 'Preparation Example 2-1' Fermented herbal medicine Comparative Example 16 1:10 mixture of 'Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-1' fermented herbal medicine Comparative Example 17 'Preparation Example 1-1' enzyme-treated product + 'Preparation Example 2-1' fermented herbal medicine 10: 1 mixture

< Experimental example 3. Evaluation of Antioxidant Activity of Mixed Compositions>

Experimental Example 3-1. ABTS radical scavenging ability

Since active oxygen is known as a substance that destroys hair roots and inhibits hair growth, the ABTS radical scavenging activity of the compositions of Examples 1 to 7 and Comparative Examples 1 to 17 was compared and investigated. 7.4mM ABTS [2,2'-azino-bis(3-ethybenzothiazoline-6-sulfonic acid) diammonium salt] and 2.6 The radical was formed by combining mM potassium persulfate and leaving it at room temperature and in the dark for 24 hours. Then, immediately before the experiment, the ABTS solution was diluted with PBS buffer (pH 7.4) so that the absorbance at 732 nm was 0.700±0.030. 50 μl of the sample was added to 950 μl of the diluted solution, reacted for 10 minutes in the dark, and then absorbance was measured at 732 nm. The activity was calculated by the formula ABTS radical scavenging ability (%) = 100-[(sample O.D. / control O.D.) x 100] and the results are shown in Table 6.

sample ABTS (%) Example 1 95.2±4.0 Example 2 80.9±5.3 Example 3 82.1±4.2 Example 4 85.4±3.9 Example 5 84.4±2.5 Example 6 90.1±1.4 Example 7 82.2±2.4 Comparative Example 1 68.2±3.2 Comparative Example 2 49.3±0.2 Comparative Example 3 42.5±4.7 Comparative Example 4 38.2±2.4 Comparative Example 5 30.4±5.0 Comparative Example 6 22.5±4.7 Comparative Example 7 21.2±1.5 Comparative Example 8 24.0±2.2 Comparative Example 9 41.1±0.3 Comparative Example 10 33.0±2.4 Comparative Example 11 42.1±1.5 Comparative Example 12 24.1±2.2 Comparative Example 13 41.2±0.4 Comparative Example 14 22.5±2.5 Comparative Example 15 24.2±2.2 Comparative Example 16 21.1±3.3 Comparative Example 17 22.0±4.8 α-tocopherol 98.5±7.7

As a result of the evaluation, as shown in Table 6, it was found that 95.2% of Example 1 had ABTS scavenging activity equivalent to 98.5% of α-tocopherol, which had a strong antioxidant activity, and the compositions of Examples 2 to 7 also had adequate antioxidant activity. confirmed

Experimental Example 2-2. electron donating ability

The electron donating activity of the compositions of Examples 1 to 7 and Comparative Examples 1 to 17 was comparatively investigated. According to the method of Biois (Biois MS. 1958 Nature 26. 1199-1200), 0.8 ml of 0.4 mM DPPH (1,1-diphenyl-2-picryl-hydrazyl) solution was added to 0.2 ml of the sample, followed by blanching for 10 minutes. left unattended After that, the absorbance was measured at 517 nm, and the activity was calculated according to the calculation formula 'electron donating activity (%) = 100 - [(sample O.D. / control O.D.) x 100]' and shown in Table 7.

sample EDA (%) Example 1 53.8±2.5 Example 2 48.2±3.4 Example 3 46.2±1.2 Example 4 43.5±1.2 Example 5 42.2±1.4 Example 6 45.5±2.6 Example 7 42.0±1.3 Comparative Example 1 35.4±1.8 Comparative Example 2 32.4±1.8 Comparative Example 3 33.5±4.7 Comparative Example 4 29.8±1.7 Comparative Example 5 27.4±1.8 Comparative Example 6 20.4±1.3 Comparative Example 7 22.3±1.2 Comparative Example 8 24.4±0.4 Comparative Example 9 33.0±1.1 Comparative Example 10 32.2±2.0 Comparative Example 11 20.3±0.1 Comparative Example 12 32.4±0.2 Comparative Example 13 30.1±1.4 Comparative Example 14 21.0±2.1 Comparative Example 15 25.1±0.8 Comparative Example 16 22.2±0.7 Comparative Example 17 25.2±1.1 α-tocopherol 55.0±1.8

In Table 7, the electron donating ability for each treatment was 53.8% in Example 1, which was equivalent to 55.0% of α-tocopherol with strong antioxidant activity. It was found that the compositions of other examples also had high electron donating activity.

Experimental Example 2-3. reducing power

Substances exhibiting reducing power act as electron donors and are known to act as secondary antioxidants by reducing intermediate products in the lipid peroxidation process. Accordingly, the reducing power of the compositions of Examples 1 to 7 and Comparative Examples 1 to 17 was comparatively investigated. In the irradiation method, 2.5 ml of 0.2M phosphate buffer (pH 6.6) and 2.5 ml of 1% potassium ferricyanide solution were added to 1 ml of the sample to measure the reducing power, followed by reaction at 50° C. for 30 minutes. Next, 2.5 ml of 10% TCA (trichloroacetica) solution was added, followed by centrifugation at 1,650 xg for 10 minutes, and 2.5 ml of distilled water and 0.5 ml of 0.1% FeCl ₂ solution were added to 2.5 ml of the supernatant, and the absorbance was measured at 700 nm. and the results are shown in Table 8.

sample O.D. (700nm) Example 1 0.87±0.07 Example 2 0.70±0.07 Example 3 0.74±0.04 Example 4 0.70±0.03 Example 5 0.69±0.03 Example 6 0.71±0.02 Example 7 0.72±0.05 Comparative Example 1 0.58±0.04 Comparative Example 2 0.54±0.02 Comparative Example 3 0.52±0.57 Comparative Example 4 0.44±0.57 Comparative Example 5 0.40±0.48 Comparative Example 6 0.32±0.53 Comparative Example 7 0.32±0.05 Comparative Example 8 0.31±0.22 Comparative Example 9 0.43±0.01 Comparative Example 10 0.55±0.34 Comparative Example 11 0.50±0.02 Comparative Example 12 0.41±0.13 Comparative Example 13 0.42±0.01 Comparative Example 14 0.33±0.22 Comparative Example 15 0.32±0.03 Comparative Example 16 0.34±0.32 Comparative Example 17 0.31±0.03

Experimental results, as shown in Table 8, as a result of comparative evaluation of the reducing power for Examples 1 to 7 and Comparative Examples 1 to 17, the reducing power in Example 1 was significantly higher, and Examples 2 to 7 also had antioxidant activity. you can see the good

< Experimental example 4 Evaluation of anti-inflammatory activity of mixed composition>

When the scalp is inflamed, the hair roots become weak, the hair becomes thin, and it is easy to lead to hair loss.

Accordingly, the anti-inflammatory activity of the compositions of Examples 1 to 7 and Comparative Examples 1 to 17 was evaluated by analyzing the ability of EAME to inhibit NO production in the mouse macrophage cell line RAW 264.7 cells stimulated with Lipopolysaccharide (LPS).

For the experimental method, Raw 264.7 cells, a murine macrophage cell line, were distributed from the Korean Cell Line Bank for the experiment, and DMEM medium containing 100 units/mL penicillin-streptomycin and 10% fetal bovine serum (FBS) was used in a 37°C 5% CO2 incubator. It was cultured in, and subculture was performed once every 2 to 3 days. After culturing Raw 264.7 cells (3 X 10 ⁵ cells/mL) for 18 hours, the extract sample (concentration: 10%) and LPS (1 μg/mL) were simultaneously treated and cultured for 24 hours, and NO generated was Griess. It was measured in the form of NO2- present in the cell culture medium using a reagent. The negative control was not treated with LPS, and the positive control was treated with only LPS, and the amount of NO produced was measured.

100 μL of cell culture supernatant and Griess reagent (1% (w/v) sulfanilamide, 0.1% (w/v) naphtylehylenediamine in 2.5% (v/v) phosphoric acid) were mixed in equal amounts and reacted in the dark at room temperature for 10 minutes. Absorbance (540 nm) was measured using an ELISA reader. The standard concentration curve was obtained by serial dilution of sodium nitrite (NaNO2) (10-100 μM).

sample NO production amount (μM) untreated group 7.08 LPS 38.74 LPS + Example 1 11.42 LPS + Example 2 12.75 LPS + Example 3 14.35 LPS + Example 4 14.34 LPS + Example 5 12.24 LPS + Example 6 13.14 LPS + Example 7 14.50 LPS + Comparative Example 1 17.27 LPS + Comparative Example 2 18.97 LPS + Comparative Example 3 21.24 LPS + Comparative Example 4 24.78 LPS + Comparative Example 5 26.61 LPS + Comparative Example 6 28.34 LPS + Comparative Example 7 29.03 LPS + Comparative Example 8 18.05 LPS + Comparative Example 9 21.12 LPS + Comparative Example 10 23.26 LPS + Comparative Example 11 22.34 LPS + Comparative Example 12 21.23 LPS + Comparative Example 13 22.40 LPS + Comparative Example 14 29.32 LPS + Comparative Example 15 31.95 LPS + Comparative Example 16 28.38 LPS + Comparative Example 17 27.31

As a result of the measurement, as shown in Table 9, the compositions of Example 1 and other Examples showed NO (Nitric oxide) production inhibitory activity, and through this, it can be seen that the antioxidant and anti-inflammatory activity are remarkably excellent.

<Experimental Example 5. Hair growth efficacy of mixed composition- in vitro experiment>

Experimental Example 5-1. Mother cell proliferation effect

Examples 1 to 7 and Comparative Examples 1 to 17 for the composition of the mother cell proliferation effect was compared and evaluated. For evaluation, hair cells (human keratatnocyte, HACAT) were cultured using MSC medium (Mesenchymal stem cell medium-Innoprot) containing 10% FBS, 1% antibiotics, and 1% growth supplement. 200 μl of the cultured parental cells 4×10 ⁴ cells/ml was dispensed into a 24 well plate and cultured for 24 hours at 37° C. under 5% CO ₂ conditions for stabilization. After 24 hours, the sample was treated with 5mg/ml, and then incubated for 72 hours at 37°C, 5% CO ₂ , then MTS sample (CellTiter 96 ^® AQueous Non-Radioactive Cell Proliferation Assay kit Cat. no. G5421, manufactured by Promega) 20 After adding μl and reacting for 1 hour in a 37°C, 5% CO ₂ Incubator, the OD value was measured at 492 nm. The OD value was converted into the actual number of cells by substituting it into the standard curve again, and then expressed as a percentage based on the control group to show the effect of promoting hair cell proliferation compared to the control group. As a control material, the same amount of PBS as compared to the sample was treated. The measurement results are shown in Table 10.

sample percentage(%) control 100.0±3.8 Example 1 139.3±3.6 Example 2 126.7±5.2 Example 3 123.6±3.3 Example 4 124.7±5.8 Example 5 124.1±2.3 Example 6 123.2±1.5 Example 7 124.0±3.6 Comparative Example 1 118.0±0.3 Comparative Example 2 115.3±2.9 Comparative Example 3 111.1±5.3 Comparative Example 4 111.4±5.7 Comparative Example 5 109.3±2.9 Comparative Example 6 104.3±2.3 Comparative Example 7 105.4±2.0 Comparative Example 8 108.2±0.3 Comparative Example 9 112.3±1.5 Comparative Example 10 108.0±2.3 Comparative Example 11 109.3±1.6 Comparative Example 12 106.3±2.4 Comparative Example 13 112.3±3.2 Comparative Example 14 103.0±4.2 Comparative Example 15 105.3±4.0 Comparative Example 16 102.6±0.9 Comparative Example 17 107.2±0.2

As shown in Table 10, the proliferation amount of hair cells was confirmed to proliferate hair cells compared to the control group in Examples. Among them, in Example 1, there was an effect of 39% or more hair cell proliferation compared to the control group.

Experimental Example 5-2. Dermal papilla cell proliferation effect

Examples 1 to 7 and Comparative Examples 1 to 17 for the composition of the mother cell proliferation effect was compared and evaluated. For evaluation, human dermal papilla cells were cultured using MSC medium (Mesenchymal stem cell medium-Innoprot) containing 10% FBS, 1% antibiotics, and 1% growth supplement. 200 μl of the cultured parental cells 4×10 ⁴ cells/ml was dispensed into a 24 well plate and cultured for 24 hours at 37° C. under 5% CO ₂ conditions for stabilization. After 24 hours, the sample was treated with 5mg/ml, and then incubated for 72 hours at 37°C, 5% CO ₂ , then MTS sample (CellTiter 96 ^® AQueous Non-Radioactive Cell Proliferation Assay kit Cat. no. G5421, manufactured by Promega) 20 After adding μl and reacting for 1 hour in a 37°C, 5% CO ₂ Incubator, the OD value was measured at 492 nm. The OD value was converted into the actual number of cells by substituting it into the standard curve again, and then expressed as a percentage based on the control group. As a control material, the same amount of PBS as compared to the sample was treated. The investigation results are shown in Table 11.

sample percentage(%) control 100.0±3.8 Example 1 139.8±5.8 Example 2 127.6±5.5 Example 3 125.6±3.7 Example 4 129.9±4.8 Example 5 125.2±2.4 Example 6 125.9±3.2 Example 7 128.3±1.6 Comparative Example 1 115.0±0.2 Comparative Example 2 119.3±2.6 Comparative Example 3 118.1±5.4 Comparative Example 4 110.4±4.7 Comparative Example 5 109.3±2.9 Comparative Example 6 104.3±2.4 Comparative Example 7 105.4±0.6 Comparative Example 8 106.3±1.2 Comparative Example 9 112.2±2.3 Comparative Example 10 112.4±0.5 Comparative Example 11 109.2±0.2 Comparative Example 12 108.5±1.3 Comparative Example 13 110.3±4.6 Comparative Example 14 105.2±2.2 Comparative Example 15 104.6±3.3 Comparative Example 16 107.0±4.4 Comparative Example 17 104.5±1.2

As a result of the investigation, as shown in Table 11, it was confirmed that the dermal papilla cells proliferated in each composition of the examples compared to the control group. Among them, Example 1 had a 39.8% flow cell proliferation effect compared to the control group.

<Experimental Example 6. Evaluation of the hair growth promoting effect of the mixed composition ( in-vivo experiment)>

Next, the hair growth promoting effect was investigated through animal experiments. Experimental animals used in this experiment were 6-week-old mouse C57BL/6 males received from Orient, and had an adaptation period of 1 week under breeding conditions at 25 ° C. and 50% humidity, and experiments were conducted at 7 weeks of age. After putting the mouse in a desiccator containing ether and anesthetizing it for 1 minute, hair was removed by hair removal on the back of the mouse to 2 cm X 4 cm using an electric clipper for animals. After 1 day, only mice whose hair cycles were in the telogen phase were re-selected for mice without wounds on their backs.

In this experiment, mice with similar characteristics such as weight and size were divided into 5 groups of 10 mice each, and then raised individually to measure the degree of hair growth.

Examples 1 to 4 and Comparative Example 7 and the positive control group (minoxidil solution) were fed cereal feed containing 5.0% by weight of the extract, and then reared for 30 days, and the hair grown at the hair removal site was separated and the weight was measured. Mice fed cereal feed not containing the extract were set as a negative control group to more clearly evaluate the hair growth promoting effect of the extract. The measurement results are shown in Table 12.

sample Hair weight (g/rat) negative control 0.0151±0.0021 positive control 0.1285±0.0044 Example 1 0.1273±0.0014 Example 2 0.0993±0.0012 Example 3 0.0893±0.0011 Example 4 0.0913±0.0012 Comparative Example 7 0.0502±0.0025

As a result of the evaluation, in particular, in Example 1, the hair weight of 0.1273 g was equivalent to that of the positive control group, and the hair growth effect was evaluated to be excellent, and it can be seen that Examples 2 to 4 also have good hair growth function.

Claims (5)

edible insect-derived ultra-high pressure and enzyme-treated products; And, kelp, black soybean, medicinal buckwheat, green tea, Rehmannia glutinosa, perilla leaf, lavender, yam, and fermented product of herbal composite extracts of turmeric; A composition for preventing hair loss or stimulating hair growth, characterized in that it contains. According to claim 1,
The edible insect is a composition for preventing hair loss or stimulating hair growth, characterized in that one or more species are selected from the group consisting of twin crickets, brown mealworm larvae and white-spotted flower radish larvae. A health functional food for preventing hair loss or promoting hair growth, characterized in that it contains the composition of claim 1. A pharmaceutical composition for preventing or treating hair loss, characterized in that it contains the composition of claim 1. A cosmetic composition for preventing hair loss or promoting hair growth, comprising the composition of claim 1.