MD4121C1 - Способ получения целлюлазо-амилазного комплекса - Google Patents
Способ получения целлюлазо-амилазного комплекса Download PDFInfo
- Publication number
- MD4121C1 MD4121C1 MDA20100101A MD20100101A MD4121C1 MD 4121 C1 MD4121 C1 MD 4121C1 MD A20100101 A MDA20100101 A MD A20100101A MD 20100101 A MD20100101 A MD 20100101A MD 4121 C1 MD4121 C1 MD 4121C1
- Authority
- MD
- Moldova
- Prior art keywords
- cellulase
- cultivation
- amylase complex
- separated
- complex
- Prior art date
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- 239000004382 Amylase Substances 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229920002472 Starch Polymers 0.000 claims abstract description 7
- 235000019698 starch Nutrition 0.000 claims abstract description 7
- 239000008107 starch Substances 0.000 claims abstract description 7
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000002028 Biomass Substances 0.000 claims abstract description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 4
- 239000007836 KH2PO4 Substances 0.000 claims abstract description 4
- 239000001110 calcium chloride Substances 0.000 claims abstract description 4
- 235000011148 calcium chloride Nutrition 0.000 claims abstract description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 4
- 238000005119 centrifugation Methods 0.000 claims abstract description 4
- 235000019441 ethanol Nutrition 0.000 claims abstract description 4
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 4
- 238000004062 sedimentation Methods 0.000 claims abstract description 4
- 235000010344 sodium nitrate Nutrition 0.000 claims abstract description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 4
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 3
- 241000335053 Beta vulgaris Species 0.000 claims description 3
- 230000002538 fungal effect Effects 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 2
- 239000011707 mineral Substances 0.000 claims description 2
- 235000010755 mineral Nutrition 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 4
- 241000233866 Fungi Species 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 2
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 abstract 1
- 235000021536 Sugar beet Nutrition 0.000 abstract 1
- 229910052564 epsomite Inorganic materials 0.000 abstract 1
- 235000019621 digestibility Nutrition 0.000 description 13
- 108090000790 Enzymes Proteins 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- 239000000203 mixture Substances 0.000 description 6
- 108010065511 Amylases Proteins 0.000 description 4
- 102000013142 Amylases Human genes 0.000 description 4
- 235000019418 amylase Nutrition 0.000 description 4
- 229940025131 amylases Drugs 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 108010059892 Cellulase Proteins 0.000 description 3
- 108010084185 Cellulases Proteins 0.000 description 3
- 102000005575 Cellulases Human genes 0.000 description 3
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 241000282887 Suidae Species 0.000 description 3
- 235000019784 crude fat Nutrition 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 229920005615 natural polymer Polymers 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000004362 fungal culture Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
Изобретение относится к биотехнологии, в частности к получению целлюлазо-амилазного комплекса, который может быть использован в животноводстве в качестве средства для повышения ассимиляции пищевых рационов.Способ получения целлюлазо-амилазного комплекса включает глубинное культивирование штамма гриба Aspergillus niger CNMN-FD-10 на питательной среде, при следующем соотношении компонентов, г/л: свекловичный жом - 10,0; крахмал - 10,0; пшеничные отруби - 20,0; KH2PO4 - 1,0; CaCl2 · 2H2O - 0,1; MgSO4 · 7H2O - 0,3; NaNO3 - 2,5; вода до 1,0 л, при pH 6,0, в течение 168 часов, после культивирования культуральную жидкость отделяют от биомассы фильтрацией или центрифугированием, целлюлазо-амилазный комплекс отделяют из культурального фильтрата путем осаждения этиловым ректификованным спиртом и центрифугируют.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20100101A MD4121C1 (ru) | 2010-09-16 | 2010-09-16 | Способ получения целлюлазо-амилазного комплекса |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20100101A MD4121C1 (ru) | 2010-09-16 | 2010-09-16 | Способ получения целлюлазо-амилазного комплекса |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD4121B1 MD4121B1 (en) | 2011-07-31 |
| MD4121C1 true MD4121C1 (ru) | 2012-02-29 |
Family
ID=45815215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20100101A MD4121C1 (ru) | 2010-09-16 | 2010-09-16 | Способ получения целлюлазо-амилазного комплекса |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4121C1 (ru) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4388C1 (ru) * | 2014-08-01 | 2016-07-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Способ получения ферментного препарата с b-глюкозидазной активностью |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0057146A2 (fr) * | 1981-01-28 | 1982-08-04 | TRANSEX S.A., Société dite: | Composition destinée à augmenter la production de lait chez les animaux en lactation et son procédé de préparation |
| JPS6075238A (ja) * | 1983-09-29 | 1985-04-27 | Toshio Tsubaki | 家畜飼料 |
| RO100891A2 (ro) * | 1988-05-06 | 1991-08-02 | Universitatea, Galati; Ro | Procedeu de obtinere a enzimelor celulozolitice |
| RO114150B1 (ro) * | 1995-12-27 | 1999-01-29 | Valentina Dan | Procedeu de obţinere a unui complex proteazo-amilazic cu culturi selecţionate de bacillus sp |
| RU2156581C2 (ru) * | 1998-11-17 | 2000-09-27 | Государственное научное учреждение - Всероссийский научно-исследовательский и технологический институт птицеводства | Способ кормления кур |
| RU2170253C1 (ru) * | 2000-06-22 | 2001-07-10 | Некоммерческое партнерство "Институт биотехнологии" | Мультиэнзимная композиция для животноводства |
| KR20030000028A (ko) * | 2002-12-02 | 2003-01-03 | 서희동 | 사료첨가제 제조 및 이용방법 |
| KR20030003470A (ko) * | 2001-07-02 | 2003-01-10 | 주식회사 진바이오텍 | 된장에서 분리한 신규한 바실러스 렌티모부스 ｇｂ-102 및이를 루핀종실배지에서 발효시킨 사료첨가물 |
| RU2236459C2 (ru) * | 2001-12-27 | 2004-09-20 | Государственное учреждение Татарский научно-исследовательский институт сельского хозяйства | Мультиэнзимная добавка для ферментативной обработки зерновых кормов |
| RU2275804C2 (ru) * | 2004-08-09 | 2006-05-10 | Государственное образовательное учреждение высшего профессионального образования "Оренбургский государственный университет" | Способ повышения продуктивности птицы |
| RU2006105357A (ru) * | 2006-02-20 | 2007-09-27 | ГНУ Всероссийский научно-исследовательский и технологический институт птицеводства (RU) | Корм для цыплят-бройлеров |
| UA29130U (ru) * | 2007-06-11 | 2008-01-10 | Btu Ct Privately Owned Entpr | Мультиэнзимная композиция мэк-бту для сельскохозяйственных животных и птицы |
| EA200801356A1 (ru) * | 2007-06-18 | 2008-12-30 | Ет Енд Дс Компани Лтд. | Пищевая добавка |
| CN101690545A (zh) * | 2009-09-07 | 2010-04-07 | 肇东市日成酶制剂有限公司 | 一种菌剂与酶复合微生态制剂的生产方法 |
| MD4072B1 (ro) * | 2010-03-12 | 2010-10-29 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Tulpina de fungi Aspergillus niger - producatoare de enzime cu activitate celulozolitica si xilanazica |
-
2010
- 2010-09-16 MD MDA20100101A patent/MD4121C1/ru not_active IP Right Cessation
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0057146A2 (fr) * | 1981-01-28 | 1982-08-04 | TRANSEX S.A., Société dite: | Composition destinée à augmenter la production de lait chez les animaux en lactation et son procédé de préparation |
| JPS6075238A (ja) * | 1983-09-29 | 1985-04-27 | Toshio Tsubaki | 家畜飼料 |
| RO100891A2 (ro) * | 1988-05-06 | 1991-08-02 | Universitatea, Galati; Ro | Procedeu de obtinere a enzimelor celulozolitice |
| RO114150B1 (ro) * | 1995-12-27 | 1999-01-29 | Valentina Dan | Procedeu de obţinere a unui complex proteazo-amilazic cu culturi selecţionate de bacillus sp |
| RU2156581C2 (ru) * | 1998-11-17 | 2000-09-27 | Государственное научное учреждение - Всероссийский научно-исследовательский и технологический институт птицеводства | Способ кормления кур |
| RU2170253C1 (ru) * | 2000-06-22 | 2001-07-10 | Некоммерческое партнерство "Институт биотехнологии" | Мультиэнзимная композиция для животноводства |
| KR20030003470A (ko) * | 2001-07-02 | 2003-01-10 | 주식회사 진바이오텍 | 된장에서 분리한 신규한 바실러스 렌티모부스 ｇｂ-102 및이를 루핀종실배지에서 발효시킨 사료첨가물 |
| RU2236459C2 (ru) * | 2001-12-27 | 2004-09-20 | Государственное учреждение Татарский научно-исследовательский институт сельского хозяйства | Мультиэнзимная добавка для ферментативной обработки зерновых кормов |
| KR20030000028A (ko) * | 2002-12-02 | 2003-01-03 | 서희동 | 사료첨가제 제조 및 이용방법 |
| RU2275804C2 (ru) * | 2004-08-09 | 2006-05-10 | Государственное образовательное учреждение высшего профессионального образования "Оренбургский государственный университет" | Способ повышения продуктивности птицы |
| RU2006105357A (ru) * | 2006-02-20 | 2007-09-27 | ГНУ Всероссийский научно-исследовательский и технологический институт птицеводства (RU) | Корм для цыплят-бройлеров |
| RU2321268C2 (ru) * | 2006-02-20 | 2008-04-10 | ГНУ Всероссийский научно-исследовательский и технологический институт птицеводства | Корм для цыплят-бройлеров |
| UA29130U (ru) * | 2007-06-11 | 2008-01-10 | Btu Ct Privately Owned Entpr | Мультиэнзимная композиция мэк-бту для сельскохозяйственных животных и птицы |
| EA200801356A1 (ru) * | 2007-06-18 | 2008-12-30 | Ет Енд Дс Компани Лтд. | Пищевая добавка |
| CN101690545A (zh) * | 2009-09-07 | 2010-04-07 | 肇东市日成酶制剂有限公司 | 一种菌剂与酶复合微生态制剂的生产方法 |
| MD4072B1 (ro) * | 2010-03-12 | 2010-10-29 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Tulpina de fungi Aspergillus niger - producatoare de enzime cu activitate celulozolitica si xilanazica |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4388C1 (ru) * | 2014-08-01 | 2016-07-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Способ получения ферментного препарата с b-глюкозидазной активностью |
Also Published As
| Publication number | Publication date |
|---|---|
| MD4121B1 (en) | 2011-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |