MD4388C1 - Способ получения ферментного препарата с b-глюкозидазной активностью - Google Patents
Способ получения ферментного препарата с b-глюкозидазной активностью Download PDFInfo
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- MD4388C1 MD4388C1 MDA20140079A MD20140079A MD4388C1 MD 4388 C1 MD4388 C1 MD 4388C1 MD A20140079 A MDA20140079 A MD A20140079A MD 20140079 A MD20140079 A MD 20140079A MD 4388 C1 MD4388 C1 MD 4388C1
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- Moldova
- Prior art keywords
- temperature
- separated
- enzymatic preparation
- glucosidase activity
- cnmn
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- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 230000000694 effects Effects 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title claims abstract description 11
- 230000002255 enzymatic effect Effects 0.000 title abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 8
- 108010047754 beta-Glucosidase Proteins 0.000 claims abstract description 8
- 102000006995 beta-Glucosidase Human genes 0.000 claims abstract description 8
- 235000019441 ethanol Nutrition 0.000 claims abstract description 8
- 235000016068 Berberis vulgaris Nutrition 0.000 claims abstract description 7
- 241000335053 Beta vulgaris Species 0.000 claims abstract description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 7
- 239000001110 calcium chloride Substances 0.000 claims abstract description 7
- 235000011148 calcium chloride Nutrition 0.000 claims abstract description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims abstract description 7
- 235000010344 sodium nitrate Nutrition 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000002028 Biomass Substances 0.000 claims abstract description 6
- 239000007836 KH2PO4 Substances 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims abstract description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 6
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 4
- 239000000725 suspension Substances 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 102000004190 Enzymes Human genes 0.000 claims description 19
- 108090000790 Enzymes Proteins 0.000 claims description 19
- 238000009331 sowing Methods 0.000 claims description 2
- 238000010899 nucleation Methods 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 17
- 108010084185 Cellulases Proteins 0.000 description 10
- 102000005575 Cellulases Human genes 0.000 description 10
- 108010059892 Cellulase Proteins 0.000 description 8
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 6
- 238000004062 sedimentation Methods 0.000 description 6
- 239000000706 filtrate Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229910052564 epsomite Inorganic materials 0.000 description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000223259 Trichoderma Species 0.000 description 2
- -1 aryl β-D-glucosides Chemical class 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000014101 wine Nutrition 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относится к биотехнологии, а именно к способу получения ферментного препарата с β-глюкозидазной активностью.Способ, согласно изобретению, предусматривает посев суспензии штамма Aspergillus niger CNMN-FD-10 в количестве 5,0% об. на питательную среду со следующим соотношением компонентов, г/л: свекловичный жом - 25,0, пшеничные отруби - 20,0, NaNO3 - 3,0, KH2PO4 - 1,0, KCl - 0,1, CaCl2 · 2H2O - 0,1, MgSO4 · 7H2O - 0,3 и вода до 1,0 л, при pH 5,5…6,0 и глубинное культивирование при температуре 28…30°C с непрерывным перемешиванием, в течение 7 дней, затем культуральная жидкость отделяется от биомассы, подкисляется до рН 3,0, обрабатывается этиловым ректификованным спиртом охлажденным до температуры -10…-12°C, в соотношении 1:2, соответственно, после чего ферментный препарат отделяется центрифугированием.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20140079A MD4388C1 (ru) | 2014-08-01 | 2014-08-01 | Способ получения ферментного препарата с b-глюкозидазной активностью |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20140079A MD4388C1 (ru) | 2014-08-01 | 2014-08-01 | Способ получения ферментного препарата с b-глюкозидазной активностью |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD4388B1 MD4388B1 (ru) | 2015-12-31 |
| MD4388C1 true MD4388C1 (ru) | 2016-07-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20140079A MD4388C1 (ru) | 2014-08-01 | 2014-08-01 | Способ получения ферментного препарата с b-глюкозидазной активностью |
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| MD (1) | MD4388C1 (ru) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BG29659A1 (en) * | 1978-08-09 | 1981-01-15 | Ljuckanov | Method of obtaining of ensime pectinesterase substances |
| DD219504A1 (de) * | 1983-12-01 | 1985-03-06 | Adw Ddr | Gewinnung pektinolytischer enzyme unter einsatz traegerfixierter schimmelpilze |
| RO100892A2 (ro) * | 1988-06-30 | 1991-03-13 | Institutul De Cercetari Si Proiectari Pentru Valorificarea Si Industrializarea Legumelor Si Fructelor, Bucuresti; Ro | Procedeu de obtinere a unei enzime pectolitice |
| CZ159796A3 (en) * | 1996-05-31 | 1997-10-15 | Mikrobiologicky Ustav Av Cr | Process of fermentation industrial production of enzyme preparation phytase |
| CN101649310A (zh) * | 2009-09-15 | 2010-02-17 | 安徽丰原发酵技术工程研究有限公司 | 一种β-葡萄糖苷酶的制备方法 |
| MD4072B1 (ro) * | 2010-03-12 | 2010-10-29 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Tulpina de fungi Aspergillus niger - producatoare de enzime cu activitate celulozolitica si xilanazica |
| CN102154249A (zh) * | 2011-04-25 | 2011-08-17 | 中国科学院微生物研究所 | 一种生产高活力β-葡萄糖苷酶的培养方法 |
| MD4121C1 (ru) * | 2010-09-16 | 2012-02-29 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Способ получения целлюлазо-амилазного комплекса |
| MD4186C1 (ru) * | 2012-02-20 | 2013-06-30 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Штамм гриба Fusarium gibbosum - продуцент кислых и нейтральных протеаз, ксиланаз и b-глюкозидаз |
-
2014
- 2014-08-01 MD MDA20140079A patent/MD4388C1/ru not_active IP Right Cessation
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BG29659A1 (en) * | 1978-08-09 | 1981-01-15 | Ljuckanov | Method of obtaining of ensime pectinesterase substances |
| DD219504A1 (de) * | 1983-12-01 | 1985-03-06 | Adw Ddr | Gewinnung pektinolytischer enzyme unter einsatz traegerfixierter schimmelpilze |
| RO100892A2 (ro) * | 1988-06-30 | 1991-03-13 | Institutul De Cercetari Si Proiectari Pentru Valorificarea Si Industrializarea Legumelor Si Fructelor, Bucuresti; Ro | Procedeu de obtinere a unei enzime pectolitice |
| CZ159796A3 (en) * | 1996-05-31 | 1997-10-15 | Mikrobiologicky Ustav Av Cr | Process of fermentation industrial production of enzyme preparation phytase |
| CN101649310A (zh) * | 2009-09-15 | 2010-02-17 | 安徽丰原发酵技术工程研究有限公司 | 一种β-葡萄糖苷酶的制备方法 |
| MD4072B1 (ro) * | 2010-03-12 | 2010-10-29 | Institutul De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Tulpina de fungi Aspergillus niger - producatoare de enzime cu activitate celulozolitica si xilanazica |
| MD4121C1 (ru) * | 2010-09-16 | 2012-02-29 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Способ получения целлюлазо-амилазного комплекса |
| CN102154249A (zh) * | 2011-04-25 | 2011-08-17 | 中国科学院微生物研究所 | 一种生产高活力β-葡萄糖苷酶的培养方法 |
| MD4186C1 (ru) * | 2012-02-20 | 2013-06-30 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Штамм гриба Fusarium gibbosum - продуцент кислых и нейтральных протеаз, ксиланаз и b-глюкозидаз |
Non-Patent Citations (1)
| Title |
|---|
| Микробные ферменты и биотехнология. Москва, Агропромиздат, 1986, р. 154-155 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD4388B1 (ru) | 2015-12-31 |
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| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |