MD4191C1 - Способ экстракции фикоцианина из биомассы водоросли Spirulina platensis - Google Patents
Способ экстракции фикоцианина из биомассы водоросли Spirulina platensis Download PDFInfo
- Publication number
- MD4191C1 MD4191C1 MDA20120003A MD20120003A MD4191C1 MD 4191 C1 MD4191 C1 MD 4191C1 MD A20120003 A MDA20120003 A MD A20120003A MD 20120003 A MD20120003 A MD 20120003A MD 4191 C1 MD4191 C1 MD 4191C1
- Authority
- MD
- Moldova
- Prior art keywords
- phycocyanin
- biomass
- spirulina platensis
- spirulina
- extracting
- Prior art date
Links
- 108010053210 Phycocyanin Proteins 0.000 title claims abstract description 40
- 239000002028 Biomass Substances 0.000 title claims abstract description 30
- 240000002900 Arthrospira platensis Species 0.000 title claims abstract description 18
- 235000016425 Arthrospira platensis Nutrition 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000000605 extraction Methods 0.000 title description 5
- 238000005119 centrifugation Methods 0.000 claims abstract description 8
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 238000010257 thawing Methods 0.000 claims abstract description 7
- 241000195493 Cryptophyta Species 0.000 claims abstract description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 11
- 239000001110 calcium chloride Substances 0.000 claims description 11
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 11
- 235000011148 calcium chloride Nutrition 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- 238000003756 stirring Methods 0.000 abstract description 9
- 230000001476 alcoholic effect Effects 0.000 abstract description 6
- 230000003078 antioxidant effect Effects 0.000 abstract description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 8
- 229940082787 spirulina Drugs 0.000 description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 8
- 239000007788 liquid Substances 0.000 description 7
- 210000002421 cell wall Anatomy 0.000 description 5
- 229920002534 Polyethylene Glycol 1450 Polymers 0.000 description 4
- 239000002202 Polyethylene glycol Substances 0.000 description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000005191 phase separation Methods 0.000 description 4
- 229920001223 polyethylene glycol Polymers 0.000 description 4
- 229910000160 potassium phosphate Inorganic materials 0.000 description 4
- 235000011009 potassium phosphates Nutrition 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
- 239000005695 Ammonium acetate Substances 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 229940043376 ammonium acetate Drugs 0.000 description 3
- 235000019257 ammonium acetate Nutrition 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 241000620196 Arthrospira maxima Species 0.000 description 1
- 241001464430 Cyanobacterium Species 0.000 description 1
- 229930182559 Natural dye Natural products 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002225 anti-radical effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 238000010364 biochemical engineering Methods 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000005115 demineralization Methods 0.000 description 1
- 230000002328 demineralizing effect Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000000576 food coloring agent Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002443 hepatoprotective effect Effects 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000978 natural dye Substances 0.000 description 1
- 230000000324 neuroprotective effect Effects 0.000 description 1
- 108060006184 phycobiliprotein Proteins 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Изобретение относится к биотехнологии, в частности к способу экстракции фикоцианина из биомассы водоросли Spirulina platensis для использования в качестве красителя с антиоксидантными свойствами.Способ, согласно изобретению, включает: деминерализацию биомассы, суспензирование в дистиллированной воде, разрушение клеток путём 2-кратного замораживания-оттаивания, экстракцию суспензии перемешиванием с 10…20% спиртовым раствором, который содержит CaCl2 в концентрации 5…10 g/l и разделение экстракта центрифугированием.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20120003A MD4191C1 (ru) | 2011-12-14 | 2011-12-14 | Способ экстракции фикоцианина из биомассы водоросли Spirulina platensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20120003A MD4191C1 (ru) | 2011-12-14 | 2011-12-14 | Способ экстракции фикоцианина из биомассы водоросли Spirulina platensis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD4191B1 MD4191B1 (en) | 2012-12-31 |
| MD4191C1 true MD4191C1 (ru) | 2013-07-31 |
Family
ID=47469599
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20120003A MD4191C1 (ru) | 2011-12-14 | 2011-12-14 | Способ экстракции фикоцианина из биомассы водоросли Spirulina platensis |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4191C1 (ru) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4360C1 (ru) * | 2013-09-23 | 2016-02-29 | Государственный Университет Молд0 | Способ получения миксоксантофилла из биомассы Spirulina platensis |
| RU2822178C2 (ru) * | 2019-01-11 | 2024-07-02 | Ферментальж | Способ выделения фикоцианинов |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01123865A (ja) * | 1987-11-10 | 1989-05-16 | Dainippon Ink & Chem Inc | 藍藻からのフィコシアニン含有青色顔料の製造方法及びその顔料を含む化粧品 |
| JPH0746993A (ja) * | 1993-05-31 | 1995-02-21 | Pentel Kk | フィコシアニンの製造方法 |
| MD1008G2 (ru) * | 1997-04-30 | 1999-06-30 | Государственный Университет Молд0 | Способ получения фикоцианина |
| CN1291616A (zh) * | 2000-10-02 | 2001-04-18 | 广东梅县梅雁蓝藻有限公司 | 高纯度高活性藻蓝蛋白的分离纯化方法 |
| CN1295080A (zh) * | 1999-11-09 | 2001-05-16 | 郑东 | 从螺旋藻中提取藻蓝蛋白的方法 |
| CN1417227A (zh) * | 2002-11-28 | 2003-05-14 | 浙江大学 | 制备强荧光藻蓝蛋白的方法 |
| CN1563083A (zh) * | 2004-03-27 | 2005-01-12 | 中国科学院水生生物研究所 | 一种水华蓝藻制备藻蓝蛋白的方法 |
| JP2006230272A (ja) * | 2005-02-24 | 2006-09-07 | Dainippon Ink & Chem Inc | 藍藻類からのフィコシアニンの抽出方法 |
| CN1958598A (zh) * | 2005-11-01 | 2007-05-09 | 高天荣 | 从螺旋藻中提取藻蓝蛋白的技术 |
| CN101003565A (zh) * | 2006-12-27 | 2007-07-25 | 山东理工大学 | 同时制备藻蓝蛋白和别藻蓝蛋白的方法 |
| MD3781G2 (ru) * | 2008-03-18 | 2009-07-31 | Государственный Университет Молд0 | Способ получения антиоксидантного термостабильного препарата из биомассы цианобактерии Spirulina platensis |
| CN101607988A (zh) * | 2009-07-03 | 2009-12-23 | 威海格瑞安生物工程有限公司 | 从螺旋藻中提取藻蓝蛋白、叶绿素和螺旋藻多糖的工艺 |
| CN101891809A (zh) * | 2010-06-18 | 2010-11-24 | 中国科学院海洋研究所 | 一种藻蓝蛋白提取物的制备和应用 |
-
2011
- 2011-12-14 MD MDA20120003A patent/MD4191C1/ru not_active IP Right Cessation
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01123865A (ja) * | 1987-11-10 | 1989-05-16 | Dainippon Ink & Chem Inc | 藍藻からのフィコシアニン含有青色顔料の製造方法及びその顔料を含む化粧品 |
| JPH0746993A (ja) * | 1993-05-31 | 1995-02-21 | Pentel Kk | フィコシアニンの製造方法 |
| MD1008G2 (ru) * | 1997-04-30 | 1999-06-30 | Государственный Университет Молд0 | Способ получения фикоцианина |
| CN1295080A (zh) * | 1999-11-09 | 2001-05-16 | 郑东 | 从螺旋藻中提取藻蓝蛋白的方法 |
| CN1291616A (zh) * | 2000-10-02 | 2001-04-18 | 广东梅县梅雁蓝藻有限公司 | 高纯度高活性藻蓝蛋白的分离纯化方法 |
| CN1417227A (zh) * | 2002-11-28 | 2003-05-14 | 浙江大学 | 制备强荧光藻蓝蛋白的方法 |
| CN1563083A (zh) * | 2004-03-27 | 2005-01-12 | 中国科学院水生生物研究所 | 一种水华蓝藻制备藻蓝蛋白的方法 |
| JP2006230272A (ja) * | 2005-02-24 | 2006-09-07 | Dainippon Ink & Chem Inc | 藍藻類からのフィコシアニンの抽出方法 |
| CN1958598A (zh) * | 2005-11-01 | 2007-05-09 | 高天荣 | 从螺旋藻中提取藻蓝蛋白的技术 |
| CN101003565A (zh) * | 2006-12-27 | 2007-07-25 | 山东理工大学 | 同时制备藻蓝蛋白和别藻蓝蛋白的方法 |
| MD3781G2 (ru) * | 2008-03-18 | 2009-07-31 | Государственный Университет Молд0 | Способ получения антиоксидантного термостабильного препарата из биомассы цианобактерии Spirulina platensis |
| CN101607988A (zh) * | 2009-07-03 | 2009-12-23 | 威海格瑞安生物工程有限公司 | 从螺旋藻中提取藻蓝蛋白、叶绿素和螺旋藻多糖的工艺 |
| CN101891809A (zh) * | 2010-06-18 | 2010-11-24 | 中国科学院海洋研究所 | 一种藻蓝蛋白提取物的制备和应用 |
Non-Patent Citations (4)
| Title |
|---|
| Cisneros M., Rito-Palomares M. A simplified strategy for the release and primary recovery of C-phycocyanin produced by Spirulina maxima. Chemical and Biochemical Engineering Quarterly, 2004, vol. 18, nr. 4, p. 385-390 * |
| Prabuthas P., Majumdar S., Srivastav P., Mishra H. Standardization of rapid and economical method for neutraceuticals extraction from algae. Journal of Stored Products and Postharvest Research, 2011, vol. 2(5), p. 93-96 * |
| Pusri J. Extraction of Phycocyanin from Spirulina for food grade. Thesys-Master of Science in Biotechnology, 1995 * |
| Valderama J., Perrut M., Majewski W. Extraction of Astaxantine and Phycocyanine from Microalge with Superficial Carbon Dioxide. J. Chem. Eng., 2003, 48(4), p. 827-830 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4360C1 (ru) * | 2013-09-23 | 2016-02-29 | Государственный Университет Молд0 | Способ получения миксоксантофилла из биомассы Spirulina platensis |
| RU2822178C2 (ru) * | 2019-01-11 | 2024-07-02 | Ферментальж | Способ выделения фикоцианинов |
Also Published As
| Publication number | Publication date |
|---|---|
| MD4191B1 (en) | 2012-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101352249B (zh) | 一种全面利用含油微藻的生产方法 | |
| US20110138682A1 (en) | Algal culture production, harvesting , and processing | |
| CN102295983A (zh) | 一种综合利用含油微藻的生产方法 | |
| CN103820270B (zh) | 一种具有生物学功效的复方树莓酒及其生产方法 | |
| KR20160019424A (ko) | 조류 세포 배양물 및 바이오매스, 지질 화합물 및 조성물, 그리고 관련 산물을 생산하는 방법 | |
| US20170305966A1 (en) | Method for producing a stable precipitate enriched in phycobiliproteins. | |
| ES2774463T3 (es) | Método para obtener una biomasa de una microalga de la especie Tetraselmis chuii enriquecida en superóxido dismutasa (SOD) | |
| KR101403511B1 (ko) | 친환경 무알콜, 수용성 프로폴리스의 제조방법 | |
| CN104046567A (zh) | 养殖微藻的方法和生产油脂的方法 | |
| CN102294022A (zh) | Sod制剂及其制取方法 | |
| US10745351B2 (en) | Method of producing phycocyanin powder | |
| KR101657943B1 (ko) | 고순도 피코시아닌 생산을 위한 2 단계 배양법 개발 | |
| MD4191C1 (ru) | Способ экстракции фикоцианина из биомассы водоросли Spirulina platensis | |
| CN105191993A (zh) | 一种植物源抑藻剂及其制备方法 | |
| CN102499415B (zh) | 绿藻精营养液及其制备方法 | |
| CN113480638A (zh) | 一种藻蓝蛋白快速提取方法 | |
| WO2012065545A1 (zh) | 一种快速积累油脂和叶黄素的微藻培养方法 | |
| CN100434528C (zh) | 一种提取藻蓝蛋白的方法 | |
| CN107058431B (zh) | 一种红景天液体深层发酵生产红景天苷的方法 | |
| CN102711515B (zh) | 含有荷叶提取物的用于水产饲料的小球藻悬浮液 | |
| Qayyum et al. | Development of cost-effective fertilizer-based media for the microalgae cultivation aimed at effective biomass production | |
| CN106755221A (zh) | 一种米卡芬净母核fr179642的制备方法 | |
| Raj et al. | Marine microalgae in food and health applications | |
| Gaikwad et al. | On occurrence of the genus Porphyridium Nageli: New to India | |
| JP5804319B2 (ja) | シフォナキサンチンおよび／またはシフォネインの製造方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |