MD4843C1 - Способ глубинного культивирования штамма Lentinus edodes (Berk.) Sing. CNMN-FB-01 - Google Patents
Способ глубинного культивирования штамма Lentinus edodes (Berk.) Sing. CNMN-FB-01Info
- Publication number
- MD4843C1 MD4843C1 MDA20210060A MD20210060A MD4843C1 MD 4843 C1 MD4843 C1 MD 4843C1 MD A20210060 A MDA20210060 A MD A20210060A MD 20210060 A MD20210060 A MD 20210060A MD 4843 C1 MD4843 C1 MD 4843C1
- Authority
- MD
- Moldova
- Prior art keywords
- cultivation
- berk
- sing
- cnmn
- lentinus edodes
- Prior art date
Links
- 240000000599 Lentinula edodes Species 0.000 title claims abstract description 19
- 235000001715 Lentinula edodes Nutrition 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 14
- 241000233866 Fungi Species 0.000 title abstract 3
- XDFCIPNJCBUZJN-UHFFFAOYSA-N barium(2+) Chemical compound [Ba+2] XDFCIPNJCBUZJN-UHFFFAOYSA-N 0.000 claims abstract description 8
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 claims abstract description 8
- 235000015097 nutrients Nutrition 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000007983 Tris buffer Substances 0.000 claims abstract description 6
- 239000007836 KH2PO4 Substances 0.000 claims abstract description 4
- 229910052564 epsomite Inorganic materials 0.000 claims abstract description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 4
- 238000011081 inoculation Methods 0.000 claims abstract description 3
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 3
- 238000013019 agitation Methods 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 235000019341 magnesium sulphate Nutrition 0.000 claims 1
- 239000002028 Biomass Substances 0.000 abstract description 21
- 235000013405 beer Nutrition 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000002417 nutraceutical Substances 0.000 abstract description 2
- 235000021436 nutraceutical agent Nutrition 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 13
- 230000015572 biosynthetic process Effects 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 235000013379 molasses Nutrition 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000221198 Basidiomycota Species 0.000 description 2
- 235000019486 Sunflower oil Nutrition 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- GWHOGODUVLQCEB-UHFFFAOYSA-N pyridine-2,6-dicarbonyl chloride Chemical compound ClC(=O)C1=CC=CC(C(Cl)=O)=N1 GWHOGODUVLQCEB-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002600 sunflower oil Substances 0.000 description 2
- ULEKGOXADQVOIF-UHFFFAOYSA-N 1,4-Benzodioxin-2(3H)-one Chemical compound C1=CC=C2OC(=O)COC2=C1 ULEKGOXADQVOIF-UHFFFAOYSA-N 0.000 description 1
- CABMTIJINOIHOD-UHFFFAOYSA-N 2-[4-methyl-5-oxo-4-(propan-2-yl)-4,5-dihydro-1H-imidazol-2-yl]quinoline-3-carboxylic acid Chemical compound N1C(=O)C(C(C)C)(C)N=C1C1=NC2=CC=CC=C2C=C1C(O)=O CABMTIJINOIHOD-UHFFFAOYSA-N 0.000 description 1
- WJJMNDUMQPNECX-UHFFFAOYSA-N Dipicolinic acid Natural products OC(=O)C1=CC=CC(C(O)=O)=N1 WJJMNDUMQPNECX-UHFFFAOYSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- FDBAIUSFRPWFTD-UHFFFAOYSA-L cobalt(2+) dithiocyanate trihydrate Chemical compound O.O.O.[Co++].[S-]C#N.[S-]C#N FDBAIUSFRPWFTD-UHFFFAOYSA-L 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- OJURWUUOVGOHJZ-UHFFFAOYSA-N methyl 2-[(2-acetyloxyphenyl)methyl-[2-[(2-acetyloxyphenyl)methyl-(2-methoxy-2-oxoethyl)amino]ethyl]amino]acetate Chemical compound C=1C=CC=C(OC(C)=O)C=1CN(CC(=O)OC)CCN(CC(=O)OC)CC1=CC=CC=C1OC(C)=O OJURWUUOVGOHJZ-UHFFFAOYSA-N 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000008659 phytopathology Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000013630 prepared media Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Изобретение относится к биотехнологии, а именно к глубинному культивированию штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01, производителя биомассы, которая может быть использована для получения лекарственных препаратов с лечебными и нутрацевтическими сойствами.Способ глубинного культивирования штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01 предусматривает инокуляцию семенного материала в количестве 10% об./об. в питательную среду, которая содержит, г/л: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, трис(2,6-диметил пиридиндикарбоксилат-1kONO)-ди-µ-(изотиоцианато-1,2kN)-(диизоцианато-2kN)барий(II)кобальт(II) 0,005-0,015, пивное сусло 5°Баллинга остальное, и культивирование при непрерывном перемешивании при температуре 28-30°Cв течение 144 часов.Технический результат изобретения состоит в сокращении продолжительности культивирования на 48 часов и повышении производства биомассы.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20210060A MD4843C8 (ru) | 2021-09-09 | 2021-09-09 | Способ глубинного культивирования штамма Lentinus edodes (Berk.) Sing. CNMN-FB-01 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20210060A MD4843C8 (ru) | 2021-09-09 | 2021-09-09 | Способ глубинного культивирования штамма Lentinus edodes (Berk.) Sing. CNMN-FB-01 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD4843B1 MD4843B1 (ru) | 2023-01-31 |
| MD4843C1 true MD4843C1 (ru) | 2023-08-31 |
| MD4843C8 MD4843C8 (ru) | 2024-02-29 |
Family
ID=85035523
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20210060A MD4843C8 (ru) | 2021-09-09 | 2021-09-09 | Способ глубинного культивирования штамма Lentinus edodes (Berk.) Sing. CNMN-FB-01 |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4843C8 (ru) |
-
2021
- 2021-09-09 MD MDA20210060A patent/MD4843C8/ru active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| MD4843C8 (ru) | 2024-02-29 |
| MD4843B1 (ru) | 2023-01-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| TC4A | Change of name of proprietor (patent for invention) |
Owner name: INSTITUTIA PUBLICA UNIVERSITATEA TEHNICA A MOLDOVEI, MD BD. STEFAN CEL MARE SI SFANT NR. 168, MD-2004, CHISINAU, REPUBLICA MOLDOVAINSTITUTIA PUBLICA UNIVERSITATEA DE STAT DIN MOLDOVA, MD STR. A. MATEEVICI NR. 60, MD-2009, REPUBLICA MOLDOVA Free format text: PREVIOUS NAME OF PROPRIETOR: INSTITUTUL DE MICROBIOLOGIE SI BIOTEHNOLOGIE, MD STR. ACADEMIEI NR. 1, MD-2028, CHISINAU, REPUBLICA MOLDOVAINSTITUTUL DE CHIMIE, MD STR. ACADEMIEI NR. 3, MD-2028, REPUBLICA MOLDOVA |