MD659G2 - Spectrophotometric method for selenium determination - Google Patents
Spectrophotometric method for selenium determinationInfo
- Publication number
- MD659G2 MD659G2 MD95-0368A MD950368A MD659G2 MD 659 G2 MD659 G2 MD 659G2 MD 950368 A MD950368 A MD 950368A MD 659 G2 MD659 G2 MD 659G2
- Authority
- MD
- Moldova
- Prior art keywords
- selenium
- solution
- determination
- ccl4
- stabilized
- Prior art date
Links
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title abstract 5
- 229910052711 selenium Inorganic materials 0.000 title abstract 5
- 239000011669 selenium Substances 0.000 title abstract 5
- 238000002798 spectrophotometry method Methods 0.000 title 1
- 239000000243 solution Substances 0.000 abstract 4
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 abstract 4
- UOFGSWVZMUXXIY-UHFFFAOYSA-N 1,5-Diphenyl-3-thiocarbazone Chemical compound C=1C=CC=CC=1N=NC(=S)NNC1=CC=CC=C1 UOFGSWVZMUXXIY-UHFFFAOYSA-N 0.000 abstract 2
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract 2
- 239000011707 mineral Substances 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- FZIPCQLKPTZZIM-UHFFFAOYSA-N 2-oxidanylpropane-1,2,3-tricarboxylic acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O FZIPCQLKPTZZIM-UHFFFAOYSA-N 0.000 abstract 1
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 abstract 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000013543 active substance Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 150000001768 cations Chemical class 0.000 abstract 1
- 238000000354 decomposition reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- SPVXKVOXSXTJOY-UHFFFAOYSA-N selane Chemical compound [SeH2] SPVXKVOXSXTJOY-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to biotechnology and may be applied for quantitative determination of the selenium within the organic substrata when receiving some of the albumens and other biological active substances, comprising selenium.The summary of the invention consists in the fact, that the proposed method for the selenium determination (IV) comprising acidic decomposition of the organic substratum, the leading of the pH mineral solution to 2,5-3,0, the separation of the selenium (IV) and its converting into the coloured complex combination with the application of the p-pa dithizone into CCl4 with the subsequent spectrophotometication of the solution, wherein pH mineral solution is stabilized by means of 0,1 M buffer solution citric acid - citric acid natrium, but the dithizone solution into CCl4 is stabilized by adding 0,2% ionol.The technical result of the invention consists in increasing the selenium separation degree from the present in the medium cations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD95-0368A MD659G2 (en) | 1995-10-16 | 1995-10-16 | Spectrophotometric method for selenium determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD95-0368A MD659G2 (en) | 1995-10-16 | 1995-10-16 | Spectrophotometric method for selenium determination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD659F1 MD659F1 (en) | 1997-01-31 |
| MD659G2 true MD659G2 (en) | 1997-07-31 |
Family
ID=19738738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MD95-0368A MD659G2 (en) | 1995-10-16 | 1995-10-16 | Spectrophotometric method for selenium determination |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD659G2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD4586C1 (en) * | 2016-07-21 | 2019-02-28 | Государственный Университет Молд0 | Spectrophotometric method for determining total selenium in the algal biomass |
-
1995
- 1995-10-16 MD MD95-0368A patent/MD659G2/en active IP Right Grant
Non-Patent Citations (3)
| Title |
|---|
| Analyst, v. 87, n.1036, 1962, Cambrige, England, C.A. Parcea, L.C. Harvey "A new fluorimetric reagent for selenium", p. 558-563. * |
| Аналитическая химия, том 21, № 4, 1966 г., "Наука", М., В.В. Ковальский, В.В. Ермаков " К определению селена в биологических объектах", с. 447-453. * |
| Микробиологическая промышленность, № 6(90) 1972 г., ОНТИТЭИмикробиопром, М., A.Г. Матросов, И.А. Решетникова, З.К. Ерошин, А.Н. Зякун "Количественное определение селена в микроорганизмах", с. 19-22. * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD659F1 (en) | 1997-01-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Granted patent for invention | ||
| IF99 | Valid patent on 19990615 |
Free format text: EXPIRES: 20151016 |