MX2010010309A - Ceculas hospederas y metodos para producir proteinas que contienen enlace de disulfuro. - Google Patents

Ceculas hospederas y metodos para producir proteinas que contienen enlace de disulfuro.

Info

Publication number
MX2010010309A
MX2010010309A MX2010010309A MX2010010309A MX2010010309A MX 2010010309 A MX2010010309 A MX 2010010309A MX 2010010309 A MX2010010309 A MX 2010010309A MX 2010010309 A MX2010010309 A MX 2010010309A MX 2010010309 A MX2010010309 A MX 2010010309A
Authority
MX
Mexico
Prior art keywords
methods
host cells
containing proteins
bond containing
disulfide bond
Prior art date
Application number
MX2010010309A
Other languages
English (en)
Inventor
Jean-Yves Francois Dubois
Roelof Hendrik Matthijs Kouwen
Jan Maarten Van Dijl
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2010010309A publication Critical patent/MX2010010309A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01008—Protein-disulfide reductase (1.8.1.8), i.e. thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01009—Thioredoxin-disulfide reductase (1.8.1.9), i.e. thioredoxin-reductase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La invención contempla células hospederas Bacillus genéticamente modificadas que tienen actividad de reductasa citoplásmica disminuida, tales como actividad TrxA disminuida, y un ácido nucleico que codifica una oxidasa heteróloga de estafilococos. La invención también proporciona métodos para producir enlace de di-sulfuro que contiene proteínas usando las células hospederas de la invención y métodos para mejorar el plegamiento de proteína usando la célula hospedera de la invención.
MX2010010309A 2008-03-26 2009-03-24 Ceculas hospederas y metodos para producir proteinas que contienen enlace de disulfuro. MX2010010309A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3960108P 2008-03-26 2008-03-26
US3961108P 2008-03-26 2008-03-26
PCT/IB2009/005368 WO2009118651A1 (en) 2008-03-26 2009-03-24 Host cells and methods of producing disulfide bond containing proteins

Publications (1)

Publication Number Publication Date
MX2010010309A true MX2010010309A (es) 2010-10-08

Family

ID=40828604

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010010309A MX2010010309A (es) 2008-03-26 2009-03-24 Ceculas hospederas y metodos para producir proteinas que contienen enlace de disulfuro.

Country Status (7)

Country Link
US (1) US8852886B2 (es)
EP (1) EP2271759B1 (es)
JP (1) JP5546037B2 (es)
CN (1) CN102016047B (es)
CA (1) CA2719559A1 (es)
MX (1) MX2010010309A (es)
WO (1) WO2009118651A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI20095615A0 (fi) * 2009-06-02 2009-06-02 Oulun Yliopisto Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä
CN102762723A (zh) * 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
DE102011008075A1 (de) * 2011-01-07 2012-07-12 Universität Bayreuth Verfahren zur rekombinanten Herstellung von mehrfach Disulfid-verbrückten Peptiden
DE102014208830A1 (de) * 2014-05-12 2015-11-12 Bayerische Motoren Werke Aktiengesellschaft Druckbehälter mit nassgewickeltem CFK
US20170087666A1 (en) 2015-03-20 2017-03-30 Technology Research Association For Future Additive Manufacturing Processing nozzle, processing head, machining apparatus, and control method and control program of processing nozzle
CN108690825B (zh) * 2017-04-06 2021-08-17 上海倍谙基生物科技有限公司 保护动物细胞培养中抗体二硫键完整性的无血清培养基
ES2944837T3 (es) 2018-11-08 2023-06-26 Sutro Biopharma Inc Cepas de E. coli con citoplasma oxidativo
CN110257347B (zh) * 2019-06-25 2021-06-22 成都英普博集生物科技有限公司 硫氧还蛋白突变体、其制备方法及其在重组融合蛋白生产中的应用
US12351850B2 (en) 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872563B1 (en) 1999-10-05 2005-03-29 President And Fellows Of Harvard College Compositions and methods for production of disulfide bond containing proteins in host cells
AU2003250080A1 (en) * 2002-07-18 2004-02-09 Vermicon Ag Oligonucleotides for detecting micro-organisms
WO2004056987A1 (en) 2002-12-23 2004-07-08 Rijksuniversiteit Groningen Modulation of the thioredoxin pathway

Also Published As

Publication number Publication date
CN102016047B (zh) 2014-05-28
US20110236926A1 (en) 2011-09-29
CN102016047A (zh) 2011-04-13
WO2009118651A1 (en) 2009-10-01
JP5546037B2 (ja) 2014-07-09
EP2271759A1 (en) 2011-01-12
JP2011515099A (ja) 2011-05-19
US8852886B2 (en) 2014-10-07
CA2719559A1 (en) 2009-10-01
EP2271759B1 (en) 2015-12-02

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