MX2017001931A - Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina. - Google Patents

Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina.

Info

Publication number
MX2017001931A
MX2017001931A MX2017001931A MX2017001931A MX2017001931A MX 2017001931 A MX2017001931 A MX 2017001931A MX 2017001931 A MX2017001931 A MX 2017001931A MX 2017001931 A MX2017001931 A MX 2017001931A MX 2017001931 A MX2017001931 A MX 2017001931A
Authority
MX
Mexico
Prior art keywords
furin
production
expression system
fully processed
mammalian expression
Prior art date
Application number
MX2017001931A
Other languages
English (en)
Other versions
MX377236B (es
Inventor
Dockal Michael
Horling Franziska
Böhm Ernst
Koehn Jadranka
Original Assignee
Baxalta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxalta Inc filed Critical Baxalta Inc
Publication of MX2017001931A publication Critical patent/MX2017001931A/es
Publication of MX377236B publication Critical patent/MX377236B/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/4846—Factor VII (3.4.21.21); Factor IX (3.4.21.22); Factor Xa (3.4.21.6); Factor XI (3.4.21.27); Factor XII (3.4.21.38)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21075—Furin (3.4.21.75)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Se describen en la presente métodos para la producción de un Factor X maduro totalmente procesado en un sistema de expresión que produce una cantidad controlada de furina entre 50 U/mL y 300 U/mL de un sobrenadante de cultivo. También se describen células transformadas, sistemas de expresión, y vectores de expresión para la expresión de furina y el Factor X.
MX2017001931A 2014-08-12 2015-08-12 Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina. MX377236B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462036438P 2014-08-12 2014-08-12
PCT/US2015/044883 WO2016025615A1 (en) 2014-08-12 2015-08-12 Production of fully processed and functional factor x in a furin-secreting mammalian expression system

Publications (2)

Publication Number Publication Date
MX2017001931A true MX2017001931A (es) 2017-06-30
MX377236B MX377236B (es) 2025-03-07

Family

ID=54035298

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017001931A MX377236B (es) 2014-08-12 2015-08-12 Produccion de un factor x totalmente procesado y funcional en un sistema de expresion en mamifero para secrecion de furina.

Country Status (14)

Country Link
US (1) US9873892B2 (es)
EP (1) EP3180427B1 (es)
JP (1) JP6793114B2 (es)
CN (1) CN107002041B (es)
AR (1) AR102048A1 (es)
AU (1) AU2015301726B2 (es)
CA (1) CA2958056C (es)
EA (1) EA035444B1 (es)
ES (1) ES2742724T3 (es)
IL (1) IL250530B (es)
MX (1) MX377236B (es)
NZ (1) NZ729729A (es)
TW (1) TWI666319B (es)
WO (1) WO2016025615A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
JP7253505B2 (ja) 2017-06-23 2023-04-06 武田薬品工業株式会社 第ｖｉｉｉ因子亜種の精製
WO2020257942A1 (en) * 2019-06-28 2020-12-30 Peel Sean A F A method for increasing yields and the specific activity of certain recombinant proteins in mammalian cells by co-expressing full-length furin
GB202018320D0 (en) * 2020-11-20 2021-01-06 Univ Newcastle Methods of producing recombinant complement proteins
CN115298204B (zh) * 2022-01-25 2023-04-25 青岛万明赛伯药业有限公司 proNGF突变体及其用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0785273A1 (en) * 1990-11-26 1997-07-23 Genetics Institute, Inc. Paired basic amino acid converting enzyme and DNA sequence encoding it
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
JPH09295945A (ja) * 1996-04-30 1997-11-18 Hoechst Yakuhin Kogyo Kk 成熟型骨誘導因子の製造方法
US5876923A (en) 1996-07-26 1999-03-02 Arch Development Corporation Herpes simplex virus ICP4 as an inhibitor of apoptosis
JP5099951B2 (ja) 1999-11-12 2012-12-19 バクスター・インターナショナル・インコーポレイテッド 副作用が減少されたヘモグロビン組成物
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics
WO2004101598A2 (en) * 2003-05-09 2004-11-25 Research Development Foundation Insertion of furin protease cleavage sites in membrane proteins and uses thereof
PL1969127T5 (pl) * 2005-12-21 2018-02-28 Cnj Holdings, Inc Metoda wytwarzania biologicznie czynnych białek zależnych od witaminy K metodami rekombinacji
DK2152735T3 (en) 2007-05-18 2015-04-20 Baxter Int METHOD FOR PRODUCING mature VWF FROM VWF PRO-PEPTIDE
MX2010007344A (es) * 2007-12-31 2010-11-09 Baxter Int Furina recombinante substancialmente libre de proteina animal y metodos para la produccion de la misma.
CN108530543B (zh) 2009-02-03 2023-06-23 阿穆尼克斯制药公司 延伸重组多肽和包含该延伸重组多肽的组合物
AU2012261813B2 (en) 2011-06-02 2017-04-20 Takeda Pharmaceutical Company Limited Formulations of recombinant furin

Also Published As

Publication number Publication date
CA2958056C (en) 2023-07-04
US9873892B2 (en) 2018-01-23
AU2015301726A1 (en) 2017-03-16
TWI666319B (zh) 2019-07-21
CN107002041B (zh) 2021-06-29
US20160046957A1 (en) 2016-02-18
CN107002041A (zh) 2017-08-01
WO2016025615A1 (en) 2016-02-18
TW201625791A (zh) 2016-07-16
IL250530B (en) 2020-03-31
ES2742724T3 (es) 2020-02-17
CA2958056A1 (en) 2016-02-18
AR102048A1 (es) 2017-02-01
IL250530A0 (en) 2017-03-30
EA201790360A1 (ru) 2017-06-30
JP2017524366A (ja) 2017-08-31
EP3180427A1 (en) 2017-06-21
NZ729729A (en) 2023-07-28
AU2015301726B2 (en) 2021-10-21
EA035444B1 (ru) 2020-06-17
EP3180427B1 (en) 2019-05-22
MX377236B (es) 2025-03-07
JP6793114B2 (ja) 2020-12-09

Similar Documents

Publication Publication Date Title
IL249828A0 (en) Limbal stem cells of cultured mammals, methods of their creation and uses thereof
MX2020000369A (es) Metodos biologicos para preparar terpenos.
MX2021004479A (es) Agentes biologicos y su uso en plantas.
UY34227A (es) Acido nucleico recombinante que codifica el gen de la toxina axmi270, vectores, células, plantas y sus métodos de empleo
MA39819A (fr) Méthodes et compositions d'immunomodulation
MX2021008142A (es) Metodos para cosechar cultivos de celulas de mamifero.
MX2016015367A (es) Métodos y sistema de separación.
MX383750B (es) Método para el cultivo de akkermansia
NZ733055A (en) Anti-c10orf54 antibodies and uses thereof
SG10201807208RA (en) Viral resistant cells and uses thereof
ES2721309T3 (es) Composición para preparar tagatosa y método para preparar tagatosa a partir de fructosa
TR201904270T4 (tr) Tanımlı koşullar altında insan pluripotent kök hücrelerinin hematoendotelyal farklılaşması için yöntemler ve materyaller.
CL2017001227A1 (es) Ganado lechero híbrido y sistemas para maximizar la ventaja híbrida.
MX2015012168A (es) Expansión de celulas madre adultas in vitro.
EP4520300A3 (en) Heart valve anchoring device
MX2016004802A (es) Anticuerpos anti-r-espondina (anti-rspo) y metodos de uso.
MX2017006269A (es) Sistemas y metodos de control adecuados para el uso con sistemas y metodos de produccion de energia.
MX2015014424A (es) Construcciones de proteinas mitocondriales y sus usos.
CL2019000069A1 (es) Sistema de sujetador y costillas estabilizadoras.
SG11201806642QA (en) Heat storage particles, method for producing same, and heat storage material
MX2023014907A (es) Forma solida de (-)-ambrox formada por una biconversion de homofarnesol en presencia de un biocatalizador.
MX360288B (es) Sistema y procedimiento de rodamientos electricamente conductores.
MY167715A (en) Power supply system control device and power supply system control method
MX2019005262A (es) Sistemas y metodos para un mantenimiento mejorado de una configuracion de campo invertido (frc) de alto rendimiento con bombeo de vacio de tipo captura de escala multiple.
AR102048A1 (es) Producción del factor x completamente procesado y funcional en un sistema de expresión de mamífero de secreción de furina

Legal Events

Date Code Title Description
FG Grant or registration
PD Change of proprietorship

Owner name: TAKEDA PHARMACEUTICAL COMPANY LIMITED