MX2020002826A - Detección de la amplificación de la recombinasa polimerasa usando la sonda de hapteno doble. - Google Patents

Detección de la amplificación de la recombinasa polimerasa usando la sonda de hapteno doble.

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Publication number
MX2020002826A
MX2020002826A MX2020002826A MX2020002826A MX2020002826A MX 2020002826 A MX2020002826 A MX 2020002826A MX 2020002826 A MX2020002826 A MX 2020002826A MX 2020002826 A MX2020002826 A MX 2020002826A MX 2020002826 A MX2020002826 A MX 2020002826A
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Mexico
Prior art keywords
dual
detection
polymerase amplification
recombinase polymerase
hapten probe
Prior art date
Application number
MX2020002826A
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English (en)
Inventor
Michael L Powell
Frank Ray Bowler
Catherine Jean Greenwood
Olaf Piepenburg
Niall A Armes
Original Assignee
Abbott Diagnostics Scarborough Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Diagnostics Scarborough Inc filed Critical Abbott Diagnostics Scarborough Inc
Publication of MX2020002826A publication Critical patent/MX2020002826A/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
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    • A61K31/00—Medicinal preparations containing organic active ingredients
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    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
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    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/11—Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11002—Exodeoxyribonuclease III (3.1.11.2)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02023—DNA-formamidopyrimidine glycosylase (3.2.2.23)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

Esta descripción se refiere a métodos y composiciones para detectar una secuencia de ácido nucleico que usa una sonda de hapteno doble. Más específicamente, la presente descripción se refiere a usar la amplificación de la recombinasa polimerasa (RPA) y una sonda de hapteno doble para detectar una secuencia de ácido nucleico objetivo. En algunos casos, la detección se hace en una banda de flujo lateral.
MX2020002826A 2017-09-14 2018-09-14 Detección de la amplificación de la recombinasa polimerasa usando la sonda de hapteno doble. MX2020002826A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762558705P 2017-09-14 2017-09-14
PCT/US2018/051078 WO2019055780A1 (en) 2017-09-14 2018-09-14 POLYMERASE RECOMBINASE AMPLIFICATION DETECTION USING DOUBLE HAPTEN PROBE

Publications (1)

Publication Number Publication Date
MX2020002826A true MX2020002826A (es) 2020-07-22

Family

ID=65630678

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MX2020002826A MX2020002826A (es) 2017-09-14 2018-09-14 Detección de la amplificación de la recombinasa polimerasa usando la sonda de hapteno doble.

Country Status (12)

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US (1) US20190078147A1 (es)
EP (2) EP4464412A3 (es)
JP (2) JP7412332B2 (es)
KR (2) KR102800957B1 (es)
CN (1) CN111201328B (es)
AU (1) AU2018334226B2 (es)
BR (1) BR112020005121A2 (es)
CA (1) CA3075629A1 (es)
ES (1) ES2991912T3 (es)
MX (1) MX2020002826A (es)
RU (1) RU2020110056A (es)
WO (1) WO2019055780A1 (es)

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DE102020103958B4 (de) * 2020-02-14 2021-08-26 Testo bioAnalytics GmbH Oligonukleotidsonde zum spezifischen Nachweis von Mikroorganismen, korrespondierendes Verfahren und Verwendung
EP3950955A1 (en) * 2020-08-06 2022-02-09 Albert-Ludwigs-Universität Freiburg Multianalyte assay for the simultaneous detection of nucleic acid and analytes
CN113552106B (zh) * 2021-07-23 2022-12-30 济南大学 一种检测ATP、谷胱甘肽、Fpg糖基化酶的通用荧光传感器
GR20220100698A (el) * 2022-08-18 2024-03-12 Πανεπιστημιο Πατρων, Μεθοδος βελτιωσης της ανιχνευσιμοτητας των δοκιμων που βασιζονται σε ταινιες ξηρων αντιδραστηριων
GB202500508D0 (en) 2025-01-15 2025-02-26 Biocrucible Ltd Molecular diagnostic device

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Publication number Publication date
RU2020110056A (ru) 2021-10-14
JP2020537499A (ja) 2020-12-24
JP2024045115A (ja) 2024-04-02
KR20250058779A (ko) 2025-04-30
JP7884496B2 (ja) 2026-07-03
AU2018334226B2 (en) 2024-07-11
EP3682029A4 (en) 2021-06-02
JP7412332B2 (ja) 2024-01-12
EP3682029A1 (en) 2020-07-22
CN111201328B (zh) 2024-07-02
KR102800957B1 (ko) 2025-04-30
WO2019055780A1 (en) 2019-03-21
BR112020005121A2 (pt) 2020-09-24
CN111201328A (zh) 2020-05-26
CA3075629A1 (en) 2019-03-21
US20190078147A1 (en) 2019-03-14
ES2991912T3 (es) 2024-12-05
EP3682029B1 (en) 2024-08-14
KR20200054268A (ko) 2020-05-19
EP4464412A2 (en) 2024-11-20
EP4464412A3 (en) 2025-02-26
AU2018334226A1 (en) 2020-04-02

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