MX2020007929A - Gen percursor del codón optimizado y gen de péptido señal de analógo de la insulina humana. - Google Patents
Gen percursor del codón optimizado y gen de péptido señal de analógo de la insulina humana.Info
- Publication number
- MX2020007929A MX2020007929A MX2020007929A MX2020007929A MX2020007929A MX 2020007929 A MX2020007929 A MX 2020007929A MX 2020007929 A MX2020007929 A MX 2020007929A MX 2020007929 A MX2020007929 A MX 2020007929A MX 2020007929 A MX2020007929 A MX 2020007929A
- Authority
- MX
- Mexico
- Prior art keywords
- gene
- insulin analogue
- signal peptide
- human insulin
- nucleic acid
- Prior art date
Links
- 239000002243 precursor Substances 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 108010076504 Protein Sorting Signals Proteins 0.000 title abstract 3
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical class C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 title abstract 3
- 108020004705 Codon Proteins 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical class N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 239000004026 insulin derivative Substances 0.000 abstract 2
- 241000235058 Komagataella pastoris Species 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 230000003248 secreting effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/84—Pichia
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Se proporciona una molécula de ácido nucleico de un gen precursor de codón optimizado y un gen de péptido señal de un análogo de insulina humana. La molécula de ácido nucleico comprende una molécula de ácido nucleico que codifica el precursor del análogo de insulina de fusión y una molécula de ácido nucleico que codifica el factor a del péptido señal de secreción de levadura. La molécula de ácido nucleico mejora la expresión del precursor del análogo de insulina en Pichia Pastoris y reduce el costo de producción del análogo de insulina humana.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810131794 | 2018-02-09 | ||
| PCT/CN2019/074384 WO2019154311A1 (zh) | 2018-02-09 | 2019-02-01 | 一种密码子优化的人胰岛素类似物前体基因和信号肽基因 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020007929A true MX2020007929A (es) | 2020-10-01 |
Family
ID=67548783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020007929A MX2020007929A (es) | 2018-02-09 | 2019-02-01 | Gen percursor del codón optimizado y gen de péptido señal de analógo de la insulina humana. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20210032307A1 (es) |
| EP (1) | EP3750998A4 (es) |
| JP (1) | JP2021513330A (es) |
| KR (1) | KR20200119237A (es) |
| CN (1) | CN111094572B (es) |
| AU (1) | AU2019218315A1 (es) |
| BR (1) | BR112020015238A2 (es) |
| CA (1) | CA3086618A1 (es) |
| MX (1) | MX2020007929A (es) |
| RU (1) | RU2020128190A (es) |
| TW (1) | TW201934752A (es) |
| WO (1) | WO2019154311A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115927360B (zh) * | 2020-05-26 | 2023-07-14 | 北京惠之衡生物科技有限公司 | 密码子优化的门冬胰岛素前体基因、重组载体、基因工程菌及其应用 |
| CN116751817B (zh) * | 2023-05-29 | 2024-07-09 | 广东东阳光药业股份有限公司 | 检测胰岛素或其类似物的生物学活性的方法及试剂盒 |
| CN117384275B (zh) * | 2023-12-13 | 2024-03-15 | 北京科为博生物科技有限公司 | 胰岛素样生长因子突变体igf1m及其应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340772C (en) * | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences |
| SI0792290T1 (en) | 1993-09-17 | 2001-12-31 | Novo Nordisk As | Acylated insulin |
| US5639642A (en) * | 1994-06-16 | 1997-06-17 | Novo Nordisk A/S | Synthetic leader peptide sequences |
| ES2258797T3 (es) | 1996-12-20 | 2006-09-01 | Novo Nordisk A/S | Proteinas con extension n-terminal expresadas en la levadura. |
| IL136770A0 (en) * | 1998-01-23 | 2001-06-14 | Novo Nordisk As | Process for making desired polypeptides in yeast |
| AR025646A1 (es) * | 2000-09-13 | 2002-12-04 | Beta Lab Sa | Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa. |
| ATE405660T1 (de) * | 2003-06-17 | 2008-09-15 | Sembiosys Genetics Inc | Verfahren zur insulinproduktion in pflanzen |
| EP2107069B1 (en) | 2003-08-05 | 2013-01-16 | Novo Nordisk A/S | Novel insulin derivatives |
| CN1873006A (zh) * | 2005-05-30 | 2006-12-06 | 上海新生源医药研究有限公司 | 一种重组人胰岛素原的生产方法 |
| KR101702342B1 (ko) * | 2009-03-12 | 2017-02-03 | 빅텍 프라이빗 리미티드 | 폴리뉴클레오타이드 및 폴리펩타이드 서열 및 이의 방법 |
| AR087433A1 (es) * | 2011-08-08 | 2014-03-26 | Merck Sharp & Dohme | Analogos de insulina n-glicosilados |
| RU2014127270A (ru) * | 2011-12-15 | 2016-02-10 | Шанхай Хэнжуй Фармасьютикал Ко., Лтд. | Аналог человеческого инсулина и его ацилированное производное |
| WO2018024186A1 (zh) | 2016-08-02 | 2018-02-08 | 江苏恒瑞医药股份有限公司 | 一种人胰岛素或其类似物的酰化衍生物 |
-
2019
- 2019-02-01 US US16/967,117 patent/US20210032307A1/en not_active Abandoned
- 2019-02-01 JP JP2020541883A patent/JP2021513330A/ja not_active Withdrawn
- 2019-02-01 EP EP19751736.0A patent/EP3750998A4/en not_active Withdrawn
- 2019-02-01 MX MX2020007929A patent/MX2020007929A/es unknown
- 2019-02-01 KR KR1020207020915A patent/KR20200119237A/ko not_active Withdrawn
- 2019-02-01 TW TW108104187A patent/TW201934752A/zh unknown
- 2019-02-01 AU AU2019218315A patent/AU2019218315A1/en not_active Abandoned
- 2019-02-01 CN CN201980004477.8A patent/CN111094572B/zh active Active
- 2019-02-01 CA CA3086618A patent/CA3086618A1/en active Pending
- 2019-02-01 BR BR112020015238-2A patent/BR112020015238A2/pt not_active IP Right Cessation
- 2019-02-01 RU RU2020128190A patent/RU2020128190A/ru unknown
- 2019-02-01 WO PCT/CN2019/074384 patent/WO2019154311A1/zh not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CA3086618A1 (en) | 2019-08-15 |
| EP3750998A4 (en) | 2022-02-23 |
| CN111094572A (zh) | 2020-05-01 |
| RU2020128190A (ru) | 2022-03-09 |
| KR20200119237A (ko) | 2020-10-19 |
| TW201934752A (zh) | 2019-09-01 |
| US20210032307A1 (en) | 2021-02-04 |
| WO2019154311A1 (zh) | 2019-08-15 |
| AU2019218315A1 (en) | 2020-07-09 |
| CN111094572B (zh) | 2023-04-04 |
| BR112020015238A2 (pt) | 2020-12-29 |
| RU2020128190A3 (es) | 2022-03-09 |
| EP3750998A1 (en) | 2020-12-16 |
| JP2021513330A (ja) | 2021-05-27 |
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